BRYOMOLECULES - D2.1 Report on phytochemical profile of 50 moss accessions

In this deliverable, we present the metabolomic profiles of 57 moss accessions derived from
axenic cultures and provided within the framework of the BRYOMOLECULES project. The table
includes the moss species, relevant additional information, sample codes, and the identified
compounds. The taxonomic identification of some of the species is still under verification and
may be subjected to revision.
Moss samples were extracted using diethyl ether followed by methanol/chloroform (2:1, v/v). In
the first step, the material was homogenized in a mortar with diethyl ether. The extracts were
transferred to vials, evaporated to dryness, and stored at −20°C until further analysis. After
evaporation of diethyl ether at room temperature in the mortar, the remaining material was re-
extracted with methanol/chloroform (2:1, v/v). The extracts were transferred to 20 mL glass vials
and subjected to ultrasound treatment for 20 minutes. Subsequently, the samples were filtered
through a 17 mm, 0.22 μm PTFE hydrophobic syringe filter, transferred to vials, and evaporated
to dryness. The dried extracts were stored at −20°C until further analysis.
Samples were analyzed using advanced mass spectrometry–based techniques. Ether extracts
were examined by gas chromatography coupled with electron impact ionization and a single
quadrupole mass analyzer (GC–MS–EI–Q), as well as by high-performance liquid
chromatography combined with high-resolution accurate mass spectrometry and electrospray
ionization using a quadrupole time-of-flight analyzer (HPLC–HRAM–MS–ESI–QToF), operated in
both negative and positive ionization modes. Methanol–chloroform extracts were analyzed
exclusively by HPLC–HRAM–MS–ESI–QToF in both negative and positive ionization modes.
Compound identification was carried out based on available spectral libraries, reference
standards, and characteristic fragmentation patterns.
The attached table lists all 57 mosses accessions. The first column provides the serial number
(No., 1–57). The second column indicates the species name, while the third column contains
relevant additional information. The fourth column presents the sample codes used throughout
all analyses. The identified compounds are reported in the fifth column.

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