TABLE 2. PCR Thermal cycling conditions for three genes: COI, 18S and 28S used to amplify Scolelepis specimens from South Africa, adapted from Surugiu et al. (2022).

		No. of cycles
Gene	Initial denaturation	Denaturation	Annealing	Extension	Final Extension
COI			38
	94°C—3 mins	94°C—30s	46°C—45s	72°C—1min	72°C—5min
18S			40
	94°C—3 mins	94°C—30s	51°C—45s	72°C—1.5min	72°C—5min
28S			40
	94°C—5 mins	94°C—20s	52°C—45s	72°C—1.5min	72°C—5min