TABLE 3. Selected characters and measurements of Trichodrilus stygodytes sp. nov., T. tenuis Hrabě and T. moravicus Hrabě, comparing original descriptions with measurements made by authors on the NMP type material and on populations inhabiting groundwaters of Spain. Abbreviations: L, length; W, width; Ø, diameter; ind, individual; ant, anterior; post, posterior; sg, segment. Roman numbers indicate the order of the segment. Note (1): measured by the authors on mated individuals or/and unmated with sperm in sperm funnels.
Characters T. stygodytes n.sp. (Spanish type collection) T. stygodytes n.sp. (Býčí Skála) (1) T. moravicus (type collection— Říčka River) (1) T. tenuis (original description) T. tenuis (4 individuals, type collection) (1) T. tenuis (Spanish collection)
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Body Ø (mm) (s. X) 0.17–0.30 0.30–0.40 (0.56 in Hrabe 1938) 0.21–0.31 (0.26–0.33 in Hrabe 1938) 0.19 0.17–0.26 (wholemounts) 0.20–0.35 (whole-mounts and dissected)
Dorsal anterior chaetae (L, µm) c.50–84 dorsals=0.80– 1.0 ventrals 77–112 dorsals = 0.80–0.96 ventrals 60–96 dorsals = 0.8–1 ventrals 64–77 40–68 41–103 (dorsals= 0.7– 0.9 ventrals)
Ventral anterior chaetae (L, µm) (55) 62–100 90–130 68–106 64–77 53–71 61–107 (grow from II–VIII)
Clitellum X–XII (anterior XIII) X–anterior XIII X–anterior XIII Indistinct X–XII (XIII)
Pharyngeal glands V–VIII IV or V–VIII V–VIII IV–VI IV–VI IV (V)–VII or VIII
Double annulation From III or IV From III From III From III From III From III or IV (V)
Atrium, total L x ampulla Ø (µm) 80–121 x 50–80 (petiolate, ampulla spherical-oval) 79–127 x 43– 80) (petiolate, ampulla oval) 110–164 x 53–91 (petiolate, ampulla spherical-oval) 73 x 58 89–98 x 36–56 (not petiolate, ampulla oval) 63–112 x 32–75 (not petiolate, ampulla oval)
Atrial duct L x Ø (µm) Short, cylindrical 30–40 x 17–30 Short, cylindrical 32–54 x 16–27 (10–17 at pore) Short, cylindrical, 31–51 x 20–27 (to 12–17 at pore) c. 30–40 x 24 c. 23–42 x 16–26
Penis Short, salient or indistinct Short, salient or indistinct Short, conical (salient in 2 individuals) No penial chamber Short, extruded through the pore in one specimen. Short, may be extruded.
Male pore on a porophore (L, µm) Yes, roundconical (15–20 high) Yes Yes, roundconical (32 µ high) Yes, minute, conical Yes, conical c. 25 µm high Yes, c. 25 µm high
Atrial musculature (µm) 2–8, to 10 at the vasa junction 2–5, to 7 at the vasa junction c. 2–3 c. 2 2–3 c.2–3
Total atrium length to body Ø ratio 0.3–0.5 0.3–0.4 c. 0.5 0.3–0.5 0.2–0.4
Prostatic cells (height, µm) To 47–80, in clusters To 43–77, in usually welldefined clusters To 80–94 Long, apical To 47–61 To (34) 54–104 diffuse or in few large clusters
Vasa deferentia junction At the base of the ampulla At the base of the ampulla Subapical or median Subapical Median to subapical
Vasa def. Ø (µm) 8–13 Penetrate in XI 10–24 Penetrate in XI 14–24 (to 8, close to atrium). Penetrate in XI Penetrate in XI 9–16 Penetrate in XI 6–14 Penetrate in XI
Spermathecal ampullae (L x W, µm) c.50–120 x 50–70 In same sg. as the pore or passing into next sg. 115–144 x 70–108 In same sg. as the pore or passing into next sg. 100–247 x 60–130 In same sg. as the pore or passing into next sg Ampullae extend into the next sg 175–232 x 58– 74 In the same sg, or passing into next sg. 88–255 x 30–120 In the same sg. or passing into next sg.
Spermathecal ducts (L x W, µm) 40–70 x 10–15 (21–30 Ø at ectal vestibule) 70–74 x 10–20 (20–33 Ø at ectal vestibule) 105–160 x 15–30 (25–35 Ø at ectal vestibule) Short, distinctly separated duct 34–48 x 9–11 (21 Ø ectally) 28–72 x 9–12 (21–27 Ø ectally)
Sites Caves and resurgence, Sta. Eufemia-Ereñozar karst (Bizkaia, Spain) Cave Býčí Skála specimens, Moravian Karst (Czech Republic, Hr 1026, 1028). All measurements in Říčka River, Moravian Karst (Czech Republic) Moravian Karst and Bohemia in Czech Republic Type collection, National Museum of Prague (Hr 1517-9 #4. Hr 1517-7 #1) Cave rivers, resurgences and wells (Spain)