Dataset used for the publication entitled "The response of mucosal colonic microbiota to probiotic and dietary intervention in vitro"

The Mucosal Simulator of Human Gastrointestinal Microbial Ecosystem (M-SHIME) has been increasingly applied across the literature to study the responses of human mucosal microbiota to various substances. This in vitro system enables multiple sampling of mucosal microbiota over a short timeframes (several days), which is not feasible in human studies. Consequently, M-SHIME provides a valuable platform for monitoring changes in mucosal microbiota over time.  

This dataset originates from a 28-day experiment in which a simulation of balanced, dietary intervention and a parallel 14-day administration of Lacticaseibacillus rhamnosus GG was applied in M-SHIME, as well as in luminal SHIME (L-SHIME). The collected data demonstrated that the microbiota in M-SHIME responded to probiotic intervention in a manner similar to that observed in the L-SHIME system, however it reacted in a distinct way to fluctuations in the dietary nutrients. Notably, the microbiota in M-SHIME responded in a way consistent with observations reported in human studies. These findings confirm the utility of the M-SHIME platform for studying the responses of human mucosal microbiota to dietary interventions.

Detailed data description was contained in the Readme File.txt. 

This research was funded by the National Science Centre, Poland 2021/05/X/NZ4/01613.

This dataset was used for the needs of a publication entitled "The response of mucosal colonic microbiota to probiotic and dietary intervention in vitro"