Published November 11, 2025 | Version v1
Dataset Open

Immunofluorescence Images of iPSC-Derived Human Intestinal Organoids (HIOs) Infected with Ebola and Marburg Viruses (Figure 6 Dataset)

  • 1. EDMO icon Boston University

Contributors

  • 1. EDMO icon Boston University

Description

Immunofluorescence images of iPSC-derived human intestinal organoids (HIOs), either mock-infected or infected with Ebola virus (EBOV) or Marburg virus (MARV), collected at Day 1 and Day 3 post-infection. These images correspond to Figure 6 in the associated manuscript.

Fluorescent markers include GFP-CDX2 (green) for gut epithelial identity, EBOV NP or MARV NP (red) for viral nucleoprotein, and DAPI (blue) for DNA. Images were captured at 20x magnification for overall visualization and 63x magnification for detailed imaging of viral inclusion bodies.

The dataset includes .tif files (some merged and some individual fluorescence channels used in the published figure panels), .czi files (multi-channel and z-stack raw microscopy data), and .gif files representing animated z-stack projections.

Files

Figure6_HIO_EBOV_Infected_Day3_Rep1.tif

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Additional details

Dates

Collected
2025-11-11
Figure 6 – Human Intestinal Organoids Infected with Filoviruses