Concerns about current food systems have prompted increased exploration of sustainable alternative protein sources, such as microalgae. This study investigated honey Chlorella vulgaris, a chlorophyll-deficient mutant, distinguished by its consumer-friendly honey colour, milder flavour and improved texture. To facilitate the nutritional transition towards this source, a standardised in vitro semi-dynamic INFOGEST digestion model was employed to analyse the digestive behaviour of C. vulgaris, focusing on the biochemical and structural changes during in vitro digestion. Gastric digestion was conducted over 67.5 min with dynamic fluid addition and gastric emptying. Results indicated slow gastric digestion of C. vulgaris due to the initially low pepsin activity and low protein solubility. Significant protein breakdown commenced when the pH dropped to 3.5. By the end of the gastric phase, 11.8 % of the protein and 3.0 % of free amine groups were released, generating new peptides of 0.3–1 kDa. Followed by 2 h static intestinal digestion, some cell structures remained intact, indicating a barrier to nutrient release. Pancreatic enzymes caused substantial protein hydrolysis, generating a higher fraction of 0.1–0.3 kDa peptides, with a notable release of essential amino acids as well as phenolic compounds. This study highlighted that protein insolubility and the cell wall structure of C. vulgaris may impede enzyme effectiveness, leading to a reduced protein breakdown. Furthermore, introduction of processing steps may enhance bioaccessibility in microalgae-derived foods, thereby contributing to the development of nutritional and sustainable food productions.
", "funding": [ { "award": { "acronym": "GIANT LEAPS", "id": "00k4n6c32::101059632", "identifiers": [ { "identifier": "https://cordis.europa.eu/projects/101059632", "scheme": "url" } ], "number": "101059632", "program": "HORIZON.2.6", "title": { "en": "Gap resolutIon in sAfety, NuTritional, alLergenicity and Environmental assessments to promote Alternative Protein utilization and the dietary Shift" } }, "funder": { "id": "00k4n6c32", "name": "European Commission" } } ], "identifiers": [ { "identifier": "https://t-stor.teagasc.ie/entities/publication/b038927b-37e6-4b8c-9e8c-8263c7aadfa6", "scheme": "url" } ], "languages": [ { "id": "eng", "title": { "en": "English" } } ], "publication_date": "2025-03", "publisher": "Zenodo", "resource_type": { "id": "publication-article", "title": { "de": "Zeitschriftenartikel", "en": "Journal article" } }, "rights": [ { "description": { "en": "The Creative Commons Attribution license allows re-distribution and re-use of a licensed work on the condition that the creator is appropriately credited." }, "icon": "cc-by-icon", "id": "cc-by-4.0", "props": { "scheme": "spdx", "url": "https://creativecommons.org/licenses/by/4.0/legalcode" }, "title": { "en": "Creative Commons Attribution 4.0 International" } } ], "title": "Semi-dynamic in vitro digestion of honey chlorella vulgaris reveals biochemical and structural insights during gastro-intestinal transit" }, "parent": { "access": { "owned_by": { "user": "1150775" }, "settings": { "accept_conditions_text": null, "allow_guest_requests": false, "allow_user_requests": false, "secret_link_expiration": 0 } }, "communities": { "default": "0b42d7e0-029a-4755-9a45-c87e950c355b", "entries": [ { "access": { "member_policy": "open", "members_visibility": "public", "record_submission_policy": "open", "review_policy": "closed", "visibility": "public" }, "children": { "allow": false }, "created": "2025-04-15T14:44:28.473812+00:00", "custom_fields": { "subjects": [ { "id": "euroscivoc:117" }, { "id": "gemet:concept/15163" }, { "id": "mesh:D006262" }, { "id": "mesh:D004044" } ] }, "deletion_status": { "is_deleted": false, "status": "P" }, "id": "0b42d7e0-029a-4755-9a45-c87e950c355b", "links": {}, "metadata": { "curation_policy": "Only materials related to the GIANT LEAPS project.
", "description": "Repository for the GIANT LEAPS project. GIANT LEAPS is accelerating the transition from animal-based to alternative dietary proteins to work towards a safe, health-promoting and sustainable food system.", "funding": [ { "award": { "id": "00k4n6c32::101059632" }, "funder": { "id": "00k4n6c32" } } ], "organizations": [ { "id": "04qw24q55" } ], "page": "This project is funded by European Union’s Horizon Europe Research and Innovation programme under Grant Agreement no. 101059632
\nIn line with the EU Green Deal objectives and the Farm to Fork Strategy, GIANT LEAPS aims to transform the European food system to make it more sustainable and healthier.
\nFood systems are responsible for a third of total GHG emissions. The current global production and consumption of animal protein account for a significant part of those emissions, causing long-term negative impacts on our health and the environment.
\nOur ambition is to substitute the consumption of traditional animal proteins in the European diets so that 50% of total protein dietary intake is derived from alternative protein sources - such as plants, microalgae, insects, and single-cell proteins - by 2030.
\nTo do so, we engage with policymakers, the production sectors and European citizens to generate key innovations, methods, datasets and information that empower all players in the food system to make the necessary decisions, investments and choices to enable a large-scale dietary shift towards alternative protein-containing foods with optimal nutritional and environmental impact.
\nThis zenodo community is used as a repository for all GIANT LEAPS output.
\nThe EU Open Research Repository serves as a repository for research outputs (data, software, posters, presentations, publications, etc) which have been funded under an EU research funding programme such as Horizon Europe, Euratom or earlier Framework Programmes.
\nThe community is managed by CERN on behalf of the European Commission.
\nZenodo’s general policies and Terms of Use apply to all content.
\nThe EU Open Research Repository accepts all digital research objects which is a research output stemming from one of EU’s research and innovation funding programmes. The funding programmes currently include:
\nHorizon Europe (including ERC, MSCA), earlier Framework Programmes (eg Horizon 2020) as well as Euratom.
\nIn line with the principle as open as possible, as closed as necessary both public and restricted content is accepted. See note on how Zenodo handles restricted content.
\nEU programme beneficiaries are eligible to submit content to the community. The community supports three types of content submissions:
\nSubmission via an EU Project Community (through user interface or programmatic APIs).
\nSubmission directly to the EU Open Research Repository.
\nAutomated harvesting from existing Zenodo content.
\nA representative of an EU project may request an EU Project Community and invite other project participants as members of the community. The project community is linked to one or more European Commission grants. All records in the project community are automatically integrated into the EU Open Research Repository immediately upon acceptance into the project community.
\nAny user may submit a record directly to the EU Open Research Repository. The submission will be moderated by Zenodo staff for compliance with the minimal required metadata requirements and its correctness.
\nRecords found among Zenodo’s existing content will on a regular basis automatically be integrated if they are found to comply with the requirements. The submissions through this method are integrated into the EU Open Research Repository with delay in a fully automated way.
\nRecords in the EU Open Research Repository are required to comply with the following minimal metadata requirements:
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\nResource types: All resource types.
\nLicenses: Public and embargoed records MUST specify a license. The chosen license SHOULD be compliant with the Horizon Europe open science requirements (see Open Science in Horizon Europe)
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\nCreators: Creators SHOULD be identified with a persistent identifier (e.g. ORCID, GND, …), and affiliations SHOULD be identified with a persistent identifier (e.g. ROR, ISNI, …)
\nSubjects: Records SHOULD specify one or more fields of science from the European Science Vocabulary.
\nThese metadata requirements comes from the related open science requirements in Horizon Europe which are detailed in each project's grant agreement.
\nAll submissions undergo automated curation checks for compliance with the policy. Submissions through project communities are in addition reviewed by the project community. Submission directly to the EU Open Research Repository is in addiotn reviewed by Zenodo staff.
\nCommunity curators may at any point edit metadata of the records in the community without notice through human or automated processing. The curators may at their sole discretion remove records from the community that are deemed not to comply with the content and curation policy or which are deemed of insufficient quality.
\nThe content and curation policy is subject to change by the community owner at any time and without notice, other than through updating this page.
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\nThe mission of the repository is to support the implementation of the EU's open science policy, providing a trusted and comprehensive space for researchers to share their research outputs such as data, software, reports, presentations, posters and more. The EU Open Research Repository simplifies the process of complying with open science requirements, ensuring that research outputs from Horizon Europe, Euratom, and earlier Framework Programmes are freely accessible, thereby accelerating scientific discovery and innovation.
\nThe EU Open Research Repository serves as a complementary platform to the Open Research Europe (ORE) publishing platform. Open Research Europe focuses on providing a publishing venue for peer-reviewed articles, ensuring that research meets rigorous academic standards. The EU Open Research Repository provides a space for all the other research outputs including data sets, software, posters, and presentations that are out of scope for ORE. This holistic approach enables researchers to not only publish their findings but also share the underlying data and materials that support their work, fostering transparency and reproducibility in the scientific process.
\nThe EU Open Research Repository is funded by the European Union under grant agreement no. 101256740 (HORIZON-ZEN Plus). For more information about the project see https://about.zenodo.org/projects/horizon-zen/.
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\nThe community is managed by CERN on behalf of the European Commission.
\nZenodo’s general policies and Terms of Use apply to all content.
\nThe EU Open Research Repository accepts all digital research objects which is a research output stemming from one of EU’s research and innovation funding programmes. The funding programmes currently include:
\nHorizon Europe (including ERC, MSCA), earlier Framework Programmes (eg Horizon 2020) as well as Euratom.
\nIn line with the principle as open as possible, as closed as necessary both public and restricted content is accepted. See note on how Zenodo handles restricted content.
\nEU programme beneficiaries are eligible to submit content to the community. The community supports three types of content submissions:
\nSubmission via an EU Project Community (through user interface or programmatic APIs).
\nSubmission directly to the EU Open Research Repository.
\nAutomated harvesting from existing Zenodo content.
\nA representative of an EU project may request an EU Project Community and invite other project participants as members of the community. The project community is linked to one or more European Commission grants. All records in the project community are automatically integrated into the EU Open Research Repository immediately upon acceptance into the project community.
\nAny user may submit a record directly to the EU Open Research Repository. The submission will be moderated by Zenodo staff for compliance with the minimal required metadata requirements and its correctness.
\nRecords found among Zenodo’s existing content will on a regular basis automatically be integrated if they are found to comply with the requirements. The submissions through this method are integrated into the EU Open Research Repository with delay in a fully automated way.
\nRecords in the EU Open Research Repository are required to comply with the following minimal metadata requirements:
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\nResource types: All resource types.
\nLicenses: Public and embargoed records MUST specify a license. The chosen license SHOULD be compliant with the Horizon Europe open science requirements (see Open Science in Horizon Europe)
\nFunding information: Records MUST specify at least one grant from the European Commission.
\nCreators: Creators SHOULD be identified with a persistent identifier (e.g. ORCID, GND, …), and affiliations SHOULD be identified with a persistent identifier (e.g. ROR, ISNI, …)
\nSubjects: Records SHOULD specify one or more fields of science from the European Science Vocabulary.
\nThese metadata requirements comes from the related open science requirements in Horizon Europe which are detailed in each project's grant agreement.
\nAll submissions undergo automated curation checks for compliance with the policy. Submissions through project communities are in addition reviewed by the project community. Submission directly to the EU Open Research Repository is in addiotn reviewed by Zenodo staff.
\nCommunity curators may at any point edit metadata of the records in the community without notice through human or automated processing. The curators may at their sole discretion remove records from the community that are deemed not to comply with the content and curation policy or which are deemed of insufficient quality.
\nThe content and curation policy is subject to change by the community owner at any time and without notice, other than through updating this page.
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\nThe mission of the repository is to support the implementation of the EU's open science policy, providing a trusted and comprehensive space for researchers to share their research outputs such as data, software, reports, presentations, posters and more. The EU Open Research Repository simplifies the process of complying with open science requirements, ensuring that research outputs from Horizon Europe, Euratom, and earlier Framework Programmes are freely accessible, thereby accelerating scientific discovery and innovation.
\nThe EU Open Research Repository serves as a complementary platform to the Open Research Europe (ORE) publishing platform. Open Research Europe focuses on providing a publishing venue for peer-reviewed articles, ensuring that research meets rigorous academic standards. The EU Open Research Repository provides a space for all the other research outputs including data sets, software, posters, and presentations that are out of scope for ORE. This holistic approach enables researchers to not only publish their findings but also share the underlying data and materials that support their work, fostering transparency and reproducibility in the scientific process.
\nThe EU Open Research Repository is funded by the European Union under grant agreement no. 101256740 (HORIZON-ZEN Plus). For more information about the project see https://about.zenodo.org/projects/horizon-zen/.
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