### README - Data and code from: Using programmable infusion pump in avian species - a validation methodology in geese

##### Data source

Data from: Using programmable infusion pump in avian species - a validation methodology in geese

Authors: Myriam Trottier-Paquet, Maude Gauthier-Bouchard, Akiko Kato, Charline Parenteau, Frédéric Angelier \& Pierre Legagneux

Article DOI: 10.1002/wlb3.01527



#### Overview

We developed a method to externally attach commercial programmable infusion pumps (iPrecio SMP-200) to birds to deliver substances subcutaneously at pre-programmed rates. We tested this method on five semi-captive Canada geese (Branta canadensis) and five greater snow geese (Anser caerulescens atlanticus) at the Zoo Sauvage de St-Félicien in April 2022. We monitored changes in body mass, wound healing, and the operational state of the pumps. We equipped the geese with Axy 5S accelerometers (TechnoSmArt, Italy) mounted collars to recorded tri-axial acceleration between -8 and +8 G at a frequency of 25Hz for 11 days. In snow geese, we conducted a hormonal experiment by delivering adrenocorticotropic hormone (ACTH) via the pumps to stimulate glucocorticoid production. We then measured body mass at three time points, recorded activity using dynamic body acceleration, and collected fecal samples daily to assess corticosterone levels (the main GC in birds).



##### Files description

1. ###### raw\_accelerometry\_data.zip

Contains raw accelerometer files (69EE89EE.txt to 80EE87EE.txt)



###### Variables:

TagID: Unique identification of the individual and the recording session of the Axy 5S accelerometer (TechnoSmArt, Italy, Frequency: 25Hz, G full scale: 8, Resolution: 8bits)

Timestamp: UTC datetime

accX: Raw acceleration along the x axis (g) along the neck

accY: Raw acceleration along the y axis (g) around the neck (interchangeable with z-axis due to the free rotation of the collar)

accZ: Raw acceleration along the z axis (g) around the neck (interchangeable with y-axis due to the free rotation of the collar)

accX\_B: Static acceleration along the x axis estimated using a smoothing function over 1 second in IGOR Pro (v 9.0.5.1 Wavemetrics, USA)

accY\_B: Static acceleration along the y axis estimated using a smoothing function over 1 second in IGOR Pro (v 9.0.5.1 Wavemetrics, USA)

accZ\_B: Static acceleration along the z axis estimated using a smoothing function over 1 second in IGOR Pro (v 9.0.5.1 Wavemetrics, USA)

pitch: Pitch angle calculated using the static acceleration

roll: Rolling angle calculated using the static acceleration

VDBA0: Vectorial dynamic body acceleration (VDBA)



###### 2\. accelero\_cago\_hour.Rds, accelero\_sngo\_hour.Rds, accelero\_cago\_hour.csv \& accelero\_sngo\_hour.csv

Derived datasets (see code\_to\_format\_raw\_accelerometry.R). Hourly summaries of acceleration data for Canada geese (cago) \& greater snow geese (sngo).



###### Variables:

TagID: Unique identification of the individual and the recording session of the Axy 5S accelerometer

Day: Local day in April 2022

Hour: Local hour in April 2022

mean\_VDBA0: Hourly mean of the vectorial dynamic body acceleration (VDBA)

sd\_VDBA0: Hourly standard variation of the VDBA

mean\_pitch: Hourly mean of the pitch angle

sd\_pitch: Hourly standard variation of the pitch angle

species: Species code with "CAGO" for Canada goose or "SNGO" for greater snow goose.

period: Experimental period : "rest" before the start of the programmed ACTH delivery, "test" during the 5 days of the programmed infusion period  \& "control" during the 5 days with pump without ACTH delivery.

sunrise: Local date time of the sunrise at St-Félicien, Qc in April 2022

sunset: Local date time of the sunset at St-Félicien, Qc in April 2022

localDateTime: Local date time (timezone Canada/Eastern)

light: "daylight" if there is light (from 30 min before sunrise to 30min after sunset) for the majority of the hour \& "night" if there is no light for the majority of the hour.

exp\_hour: Experimental hour with 0 being the start of the programmed ACTH delivery.

exp\_day: Experimental day with 0 being the rest period, 1 to 5 being the test period and 6 to 10 being the control period.



###### 3\. data\_mass.Rds \& data\_mass.csv

Body mass and morphometrics.



###### Variables:

date: Local date of the mass measurement

id: Unique identification of the individual

Sp: Species code with "CAGO" for Canada goose or "SNGO" for greater snow goose

sexe: Sex of the individual

age: Age of the individual

date\_chir: Date of the surgery

tete: Head lenght (mm)

tarse: Tarsus lenght (mm)

masse: Body mass (g)

jour\_trt: Number of days since the beginning of the experiment with 0=day of the surgery

Outcome: Outcome of the experiment with "success" for a pump that delivered the ACTH until the removal of the pump and "failure" for a pump that did not deliver the ACTH or that is not functional by the end.



###### 4\. data\_cort.Rds \& data\_cort.csv

Corticosterone levels from fecal samples.



###### Variables:

sample\_id: individual number of the 100 mg dried and homogenized dropping sample

cort: corticosterone concentration (pg/mg). Intra essay precision = 10,3%, inter-assay precision = 10,62%, LDL = 0,23 pg/mg

date: collection date (local)

species: species in the enclosure where feces samples were collected

individual\_id: individual number of the dropping sample used for the cort assay

mass: Mass (g) of dried and homogenized fecal sample (Sartorius SECURA-224-1S balance)

process\_date: Date at which dried samples were homogenized, measured and packed for corticosterone assay (timezone Canada/Eastern)

date\_chir: Date of the surgery (timezone Canada/Eastern)

date\_inf: Date and time of the start of the infusion (timezone Canada/Eastern)

time\_date: Date and time of the dropping sample collection (timezone Canada/Eastern)

jour: Number of days since the beginning of the experiment with 0=day of the surgery

phase: Experimental period with rest=pre-infusion, test=infusion and control=post-infusion

dose: Daily dose (mg/kg/day)

tps: Experimental day within period



##### Code

###### 5\. code\_pump\_wildlife\_biology.R

R script to reproduce the main analyses and figures from the article.



###### 6\. code\_to\_format\_raw\_accelero.R

R script to format the raw accelerometry data into hourly summaries of the acceleration.



##### Notes

Time convention: Raw accelerometer data in UTC; all derived datasets and other files in local time (Canada/Eastern)

Formats: .Rds and .csv contain the same data (R binary vs plain text)



##### Software

The analyses were conducted using R statistical software (v4.2.2; R Core Team, 2022)