Published May 17, 2025 | Version v2
Journal article Open

Allosteric regulation of the Golgi-localized PPM1H phosphatase by Rab GTPases modulates LRRK2 substrate dephosphorylation in Parkinson's disease

Creators

  • 1. ROR icon Stanford Medicine

Description

    1. Figure 2.zip
      Contains all raw data and analysis related to Microscale Thermophoresis (MST) binding experiments between wild-type (WT) PPM1H or L66R PPM1H and Rab8A, Rab10, and Rab12. This includes:
      1. CSV files of raw MST measurements
      2. GraphPad Prism files used to generate binding curves
    2. Figure 3.zip
      Contains MST data for WT, L66R and R338A PPM1H binding to phosphorylated Rab8A (pRab8A), unphosphorylated Rab8A and GTP/GDP bound wild type Rab8A
      1. Corresponding Prism files for curve fitting and analysis
      2.  CSV files of raw MST experiments
    3. Figure 4.zip
      Contains data for co-flotation assays of WT and L66R PPM1H with Rab8A (Q67L), including:
      1. Primary immunoblot images (TIFF format)
      2. Annotated blot images (PDF)
      3. Gel quantification data (CSV)
      4. GraphPad Prism analysis files
    4. Figure 5.zip
      Co-flotation data for WT and L66R PPM1H with Rab10 (Q68L):
      1. Primary and annotated blot images
      2. Quantification (CSV)
      3. Prism analysis files
    5. Figure 6.zip
      Contains data for       D37 PPM1H experiments:
      1. Co-flotation with Rab8A (TIFF and annotated immunoblots, CSV quantitation, Prism analysis)
      2. MST binding experiments of D37 PPM1H with Rab8A and Rab10 (CSV files, Prism files)
    6. Figure 7.zip
      Data for the inhibition of PPM1H activity by unphosphorylated Rab8A and the comparison of phosphatase activity of WT, L66R and       D37 PPM1H:
      1. Immunoblot data (TIFF and annotated PDF)
      2. Gel quantification (CSV)
      3. Prism files analyzing the inhibition curve
    7. Figure S2. zip       containing the raw data for the Phos-tag gel along with the corresponding quantification in CSV format (analyzed in Prism). It also includes the CSV, Prism project file, and the original TIFF western blot images for the control flotation experiment with Rab8A alone, both with and without liposome.
    8. Figure S3. zip
      Co-flotation assay data for WT and L66R PPM1H with Rab12:
      1. Raw immunoblots (TIFF), annotated PDFs
      2. Quantification and Prism analysis files

     

     

     

Files

Fig 3.zip

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