19/22 °C55% RHScanningwater deficitcontrolClimate chamber with plant screening set-upabCCD0D1D2D3D4D5D6D7D8D9D10D11D12D13D14D152VNIR imaging:Fm’ 300Fm’ 700Fm’ 1100Fm’ 1500c8 h darkDaily protocols1PAM F imaging:RdarkR300R700R1100R1500ΔAΔAΔAΔACumulativeFm, Fm’Fo, Fo’Measurement orderStepwisePAR100PAR300 PAR500 PAR700PAR900PAR1100PAR1300 PAR1500ΔAQYdarkQY300QY700QY1100QY1500StressRecoveryLeaf spectroscopy + pigments12Treatments and samplingAdarkA300A700A1100A1500121 PAM fluorescence unit2 VNIR hyperspectral cameraCWatering (c: control)C19/22 °C55% RHScanningwater deficitcontrolClimate chamber with plant screening set-upabCCD0D1D2D3D4D5D6D7D8D9D10D11D12D13D14D152VNIR imaging:Fm’ 300Fm’ 700Fm’ 1100Fm’ 1500c8 h darkDaily protocols1PAM F imaging:RdarkR300R700R1100R1500ΔAΔAΔAΔACumulativeFm, Fm’Fo, Fo’Measurement orderStepwisePAR100PAR300 PAR500 PAR700PAR900PAR1100PAR1300 PAR1500ΔAQYdarkQY300QY700QY1100QY1500StressRecoveryLeaf spectroscopy + pigments12Treatments and samplingAdarkA300A700A1100A1500121 PAM fluorescence unit2 VNIR hyperspectral cameraCWatering (c: control)C19/22 °C55% RHScanningwater deficitcontrolClimate chamber with plant screening set-upabCCD0D1D2D3D4D5D6D7D8D9D10D11D12D13D14D152VNIR imaging:Fm’ 300Fm’ 700Fm’ 1100Fm’ 1500c8 h darkDaily protocols1PAM F imaging:RdarkR300R700R1100R1500ΔAΔAΔAΔACumulativeFm, Fm’Fo, Fo’Measurement orderStepwisePAR100PAR300 PAR500 PAR700PAR900PAR1100PAR1300 PAR1500ΔAQYdarkQY300QY700QY1100QY1500StressRecoveryLeaf spectroscopy + pigments12Treatments and samplingAdarkA300A700A1100A1500121 PAM fluorescence unit2 VNIR hyperspectral cameraCWatering (c: control)CSchematic representation of the experimental design and measurement workflow (as published in Figure 1, García-Martínez et al., 2025).