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GENERAL INFORMATION 
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This readme file was generated on 2025-07-10 by Mengjun Sheng

Title of Dataset: Two photon raw images and calcium trace and behavior
Description of Dataset: This dataset contains raw two photon images, and calcium trace and behavior data
Principal Investigator: Jun Ding
Stanford University School of Medicine: Stanford, CA, US
0000-0003-0690-1312

Date of Data Collection: listed in the file name
Software Dependencies: Photoshop, ImageJ, Matlab

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FILE OVERVIEW 
uploaded in four Zenodo links:
1.https://zenodo.org/records/15837908
2.https://zenodo.org/records/15844354
3.https://zenodo.org/records/15839368
4.https://zenodo.org/records/15844411
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Directory of Files: 
2P_raw and calcium trace and behavior_Part1
2P_raw and calcium trace and behavior_Part2
2P_raw and calcium trace and behavior_Part3
2P_raw and calcium trace and behavior_Part4
2P_raw and calcium trace and behavior_Part5
2P_raw and calcium trace and behavior_Part6
2P_raw and calcium trace and behavior_Part7
2P_raw and calcium trace and behavior_Part8
2P_raw and calcium trace and behavior_Part9
2P_raw and calcium trace and behavior_Part10
2P_raw and calcium trace and behavior_Part11
2P_raw and calcium trace and behavior_Part12
2P_raw and calcium trace and behavior_Part13
2P_raw and calcium trace and behavior_Part14
2P_raw and calcium trace and behavior_Part15
2P_raw and calcium trace and behavior_Part16
2P_raw and calcium trace and behavior_Part17
2P_raw and calcium trace and behavior_Part18
2P_raw and calcium trace and behavior_Part19
2P_raw and calcium trace and behavior_Part20
2P_raw and calcium trace and behavior_Part21
2P_raw and calcium trace and behavior_Part22
2P_raw and calcium trace and behavior_Part23
2P_raw and calcium trace and behavior_Part24
2P_raw and calcium trace and behavior_Part25
2P_raw and calcium trace and behavior_Part26
2P_raw and calcium trace and behavior_Part27
2P_raw and calcium trace and behavior_Part28
2P_raw and calcium trace and behavior_Part29
2P_raw and calcium trace and behavior_Part30
2P_raw and calcium trace and behavior_Part31
2P_raw and calcium trace and behavior_Part32
2P_raw and calcium trace and behavior_Part33
2P_raw and calcium trace and behavior_Part34
2P_raw and calcium trace and behavior_Part35
2P_raw and calcium trace and behavior_Part36
2P_raw and calcium trace and behavior_Part37
2P_raw and calcium trace and behavior_Part38
2P_raw and calcium trace and behavior_Part39
2P_raw and calcium trace and behavior_Part40
2P_raw and calcium trace and behavior_Part41
2P_raw and calcium trace and behavior_Part42
2P_raw and calcium trace and behavior_Part43
2P_raw and calcium trace and behavior_Part44
2P_raw and calcium trace and behavior_Part45
2P_raw and calcium trace and behavior_Part46
2P_raw and calcium trace and behavior_Part47
2P_raw and calcium trace and behavior_Part48
2P_raw and calcium trace and behavior_Part49
2P_raw and calcium trace and behavior_Part50
2P_raw and calcium trace and behavior_Part51
2P_raw and calcium trace and behavior_Part52
2P_raw and calcium trace and behavior_Part53
2P_raw and calcium trace and behavior_Part54

File Formats: .tiff and .raw images  and .txt and .mat
File Naming Convention: Day+mice
                                              Day+mice+ROI


DATA SPECIFIC INFORMATION FOR Ca traces
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Date of Creation: 20250710
Description of Data: Calcium transients of axonal boutons
Number of Variables: n boutons and n time
Variable List:
	A. Time: frame
	B. Calcium fluorescence changes

DATA SPECIFIC INFORMATION FOR behavior
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Date of Creation: 20250710
Description of Data: behavior data (lever pushing movement)
Number of Variables: 5
Variable List:  Cue, reward, punishment, lever pushing movement, Time

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DATA SPECIFIC INFORMATION FOR raw images
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Date of Creation:  20250710
Description of Data: Two photon raw images of corticostriatal axons and boutons
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METHODOLOGY
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Description of methods used for data collection: Thorlabs B-scope, ThorImage

Description of methods used for data processing: Manully idebtify axon and bouton using photoshop or imagej

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DATA ACCESS AND SHARING
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Publications based on this dataset: Mengjun Sheng et al. Nature 2025
Recommended citation for this dataset: Mengjun Sheng et al. Nature 2025

License information: CC-BY 4.0