Key Resource Table,,,,,
,,,,,
Resource Type ,Resource Name,Source ,Identifiers,New/ Reuse,Additional information
Genetic reagent (Mus musculus),C57BL/6N-Gba1tm1.1Mjff/J,The Jackson Laboratory,RRID:IMSR_JAX:019 106,Reuse,GBA1 D409V HOM
Antibody,anti-Arl13B (mouse monoclonal),Neuromab,N295B/66 RRID:AB_2877361,Reuse,"IHC-(1:500),
IF-(1:1000)"
Antibody,anti-Choline Acetyltransferase (goat polyclonal),Millipore,AB144P-1ML RRID:AB_2079751,Reuse,(1:200)
Antibody,anti-ALDH1L1 (rabbit polyclonal),Proteintech,17390-1-AP RRID:AB_2878401,Reuse,(1:200)
Antibody,anti-Adenylate cyclase III (rabbit polyclonal),EnCOR,RPCA-ACIII RRID:AB_2572219,Reuse,(1:10000)
Antibody,"anti-Glucocerebrosidase (C-terminal, rabbit polyclonal)",Sigma-Aldrich,"G4171
RRID:AB_1078958",New,(1:1000)
Antibody,anti-alpha tubulin (mouse monoclonal),Santa Cruz Biotechnology,sc-32293 RRID:AB_628412,Reuse,(1:4000)
Antibody,H+L Donkey anti-Goat Alexa 488,Life Technologies,A11055 RRID:AB_2534102,Reuse,(1:2000)
Antibody,H+L Donkey anti-Mouse Alexa 568,Life Technologies,"A10037
RRID:AB_11180865",Reuse,(1:2000)
Antibody,H+L Donkey anti-Mouse Alexa 488,Life Technologies,"A32766
RRID: AB_2762823",Reuse,(1:2000)
Antibody,IRDye 800CW Donkey anti rabbit IgG,LI-COR,"926-32213
RRID:AB_621848",Reuse,"(1:10,000)"
Antibody,IRDye 680RD Donkey anti mouse IgG,LI-COR,"926-68072
RRID:AB_10953628",Reuse,"(1:10,000)"
Antibody,H+L Donkey anti-Rabbit Alexa 568,Life Technologies,A10042 RRID:AB_2534017,Reuse,(1:2000)
Commercial assay or kit,"RNAscopeMultiplex Fluorescent Reagent Kit
v2",Advanced Cell Diagnostics,#323100,Reuse,
Commercial assay or kit,RNAscope Probe-Mm- Gdnf,Advanced Cell Diagnostics,#421551,Reuse,(1:10)
Commercial assay or kit,RNAscope Probe- Mm- Ptch1-C2,Advanced Cell Diagnostics,#402811-C2,Reuse,(1:5)
Commercial assay or kit,RNAscope Probe- Mm- Bdnf,Advanced Cell Diagnostics,#424821,New,
Commercial assay or kit,OPAL 570 REAGENT PACK,Akoya Biosciences,FP1488001KT,Reuse,
Commercial assay or kit,OPAL 690 REAGENT PACK,Akoya Biosciences,FP1497001KT,Reuse,
Commercial assay or kit,High-Capacity cDNA Reverse Transcription Kit,Applied Biosystems,4368814,Reuse,
"Software, Algorithm",FIJI,PMID:29187165,RRID:SCR_002285,Reuse,
"Software, Algorithm",Prism,"Prism 10 version
10.2.3",RRID:SCR_002798,Reuse,
"Software, Algorithm",ZEN,Zeiss ZEN Microscopy Software,"RRID:SCR_013672
www.zeiss.com/micros copy/en/products/softw are/zeiss-zen.html",Reuse,
"Software, Algorithm",R,http://www.r-project.org/,RRID:SCR_001905,Reuse,
"Software, Algorithm",Hisat2 v2.0.5,,RRID:SCR_015530,Reuse,
"Software, Algorithm",featureCounts,v1.5.0-p3 PMID: 24227677,RRID:SCR_012919,Reuse,
"Software, Algorithm",DESeq2,"Rpackage (1.20.0)
doi.org/10.1186/s 13059-014-0550-
8",RRID:SCR_015687,Reuse,
"Software, Algorithm",Gene Ontology,http://www.geneontology.org/, RRID:SCR_002811,Reuse,
"Software, Algorithm",clusterProfiler,R package doi:10.1016/j.xin n.2021.100141.,RRID:SCR_016884,Reuse,
"Software, Algorithm",CellProfiler version 4.2.6,PMID:29969450,RRID:SCR_007358,Reuse,
"Software, Algorithm",Adobe Illustrator,Version 27.5,RRID:SCR_010279,Reuse,
"Chemical compound, drug",Conduritol B epoxide (CBE),Cayman Chemicals,15216,Reuse,"100µM, 7 d"
"Chemical compound, drug",SAG,Cayman Chemicals,11914,Reuse,"25nM, 24 h"
"Chemical compound, drug",U-18666A,Cayman Chemicals,10009085,Reuse,"1µM, 24 h"
"Chemical compound, drug",Myriocin,Cayman Chemicals,63150,Reuse,"80 µM, 48 h"
"Chemical compound, drug",Cholesterol,Sigma-Aldrich,C8667,Reuse,
"Chemical compound, drug",Methyl-β-cyclodextrin,Sigma-Aldrich,C4555,Reuse,
"Chemical compound, drug","SHH mouse Sonic hedgehog (residues 40–
194, GenBank NP_033196)","Gift from Dr. Rajat Rohatgi, Stanford","DOI
https://doi.org/10.1038/ nsmb.1607",Reuse,"10nM, 24h"
"Chemical compound, drug",PowerUp SYBR Green Master Mix,Applied Biosystems by Thermo Fisher Scientific,A25742,Reuse,
"Chemical compound, drug",Trizol reagent,Invitrogen,15596026,Reuse,
"Chemical compound, drug",RNase OUT recombinant,Invitrogen,100000840,Reuse,
"Chemical compound, drug","Paraformaldehyde Solution, EM grade",Electron Microscopy Sciences,15710,Reuse,
"Chemical compound, drug",DMEM high glucose,Cytiva,SH30243.02,Reuse,
"Chemical compound, drug",Penicillin/Streptomycin,Cytiva,SV30010,Reuse,
"Chemical compound, drug",Fetal calf serum,Sigma,F0926,Reuse,
"Chemical compound, drug",Polybrene,Sigma,TR-1003-G,Reuse,
"Chemical compound, drug",Polyethylenimine (PEI),Polysciences,23966 (1),Reuse,
Cell line (mouse),NIH-3T3-flpin,Thermo Fisher Scientific,R76107 (RRID:CVCL_U422),Reuse,
Cell line (mouse),MEF WT,MRC-PPU,from RRID: MMRRC_049312- UCD,Reuse,
Cell line (mouse),MEF GBA1 D409V Hom,MRC-PPU,from RRID: MMRRC_049312- UCD,Reuse,
Cell line (human),HEK293T,ATCC,RRID:CVCL_0063,Reuse,
Cell line (human),Fibroblast_healthy individual,Coriell Institute for Medical Research #GM08399,RRID:CVCL_7482,Reuse,
Cell line (human),Fibroblast_healthy individual,Coriell Institute for Medical Research #GM04501,RRID:CVCL_7409,Reuse,
Cell line (human),"Fibroblast_Gaucher disease, type I",Coriell Institute for Medical Research #GM10915,RRID:CVCL_0R39,Reuse,GBA1 L444P
Cell line (human),"Fibroblast_Gaucher disease, type I",Coriell Institute for Medical Research #GM01607,RRID:CVCL_7326,Reuse,"GBA1 N370S
and V394L"
Cell line (human),"Fibroblast_Gaucher disease, type II",Coriell Institute for Medical Research #GM00878,RRID:CVCL_0R28,Reuse,"GBA1 L444P
and A456P"
"Recombinant DNA
reagent",PsPAX2,Addgene,RRID:Addgene_12260,Reuse,
"Recombinant DNA
reagent",VSV-G,Addgene,RRID:Addgene_12259,Reuse,
"Recombinant DNA
reagent",mApple-SSTR3-N-17,Addgene,RRID:Addgene_54949,Reuse,
Raw data for figures_this paper,,Zenodo,"https://doi.org/10.5281/zenodo.12753029, https://doi.org/10.5281/zenodo.15801264",New,
Protocol,Small scale Lentivirus Production and Infection,Protocols.io Aligning Science Across Parkinson’s,https://dx.doi.org/10.1 7504/protocols.io.bp2l 61z2zvqe/v1,Reuse,
Protocol,Detection of accessible cholesterol in primary cilia using purified His- mNeon-ALOD4 inNIH- 3T3 Fibroblasts,Protocols.io Aligning Science Across Parkinson’s,https://dx.doi.org/10.17 504/protocols.io.rm7vz x2qxgx1/v1,Reuse,
Protocol,An ImageJ macro for the quantification of ciliary accessible cholesterol V.2,"Protocols.io Aligning Science Across
Parkinson’s",https://dx.doi.org/10.17 504/protocols.io.q26g7 1d88gwz/v2,Reuse,
Protocol,"Immunostaining and quantification of intracellular accessible cholesterol using His- mNeon-ALOD4 in
Human Fibroblasts",Protocols.io Aligning Science Across Parkinson’s,https://dx.doi.org/10.17 504/protocols.io.rm7vzj 5prlx1/v1,Reuse,
Protocol,Immunofluorescent Staining of phosphoRab10 in cultured cells,Protocols.io Aligning Science Across Parkinson’s,https://dx.doi.org/10.17 504/protocols.io.ewov1 nmzkgr2/v1,Reuse,
Protocol,"A method for RNA extraction, cDNA synthesis and and quantitative PCR from NIH-3T3 fibroblasts",Protocols.io Aligning Science Across Parkinson’s,https://dx.doi.org/10.17 504/protocols.io.rm7vzj o84lx1/v1,Reuse,
Protocol,PCR and analysis,Protocols.io Aligning Science Across Parkinson’s,https://dx.doi.org/10.17 504/protocols.io.kxygx ypwdl8j/v1,Reuse,
Protocol,Creation of pooled CRISPR KO cell lines using Synthego sgRNA,Protocols.io Aligning Science Across Parkinson’s,https://dx.doi.org/10.17 504/protocols.io.bp2l6d qodvqe/v1,Reuse,
Protocol,Immunohistochemistry in Rodent Brain,Protocols.io Aligning Science Across Parkinson’s,dx.doi.org/10.17504/pr otocols.io.bnwimfce,Reuse,
Protocol,Fluorescence in situ hybridization (FISH),Bioprotocol,https://bio-protocol.org/exchange/protocoldetail?id=1423&type=3,Reuse,