Published June 9, 2025
| Version v2
Journal article
Open
A guide to selecting high-performing antibodies for CD44 (UniProt ID: P16070) for use in western blot, immunoprecipitation, immunofluorescence, and flow cytometry
Creators
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1.
Montreal Neurological Institute and Hospital
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2.
University of Leicester
Description
This report provides a guide to selecting high-quality commercial antibodies against CD44 for use in western blot, immunoprecipitation, immunofluorescence, and flow cytometry, utilizing a standardized experimental protocol that compares readouts in knockout cell lines and isogenic parental controls. This work was supported by a grant from the Government of Canada through Genome Canada, Genome Quebec, and Ontario Genomics.
Files
CD44_YCharOS_Zenodo 2025 - v5.pdf
Files
(6.2 MB)
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Additional details
Funding
- National Institutes of Health
- Infrastructure to improve reliability of AMP-AD target hypotheses 3RF1AG057443-01S2