-------------------- GENERAL INFORMATION -------------------- This readme file was generated on 2025-05-21 by Eddy Albarran Title of Dataset: Source Data for Albarran et al. Description of Dataset: This dataset includes the source data for outputs from electrophysiology recordings that were used in the summary statistics and comparisons in the figures of manuscript. In addition, it includes a table of the genetic sequence information used in the generation and characterization of the knock-in lines. Principal Investigator: Rui M. Costa (https://orcid.org/0000-0003-0495-8374) Date of Data Collection: June 2021 to May 2025 Software Dependencies: Microsoft Excel ------------- FILE OVERVIEW ------------- Directory of Files: 'SOURCE_DATA_FIG_4.xlsx', 'SOURCE_DATA_FIG_5.xlsx', 'SOURCE_DATA_FIG_6.xlsx', 'SEQUENCES.xlsx', Relationship Between Files: No relationship. File Formats: .xlsx File Naming Convention: If the file has FIG_X in the name, it contains the source data corresponding to Figure X in the manuscript. The 'SEQUENCES' file is a standalone file. ---------------------------------------- DATA SPECIFIC INFORMATION FOR 'SOURCE_DATA_FIG_4.xlsx' ---------------------------------------- Date of Creation: 2025-05-20 Description of Data: Electrophysiological parameters from whole-cell recordings + current injections from striatal D1-Cre and A2a-Cre neurons. Number of Variables: 4 Variable List: A. Number of action potentials in response to current injected (f-I) Description: Each recorded neuron's number of action potentials evoked by a set amount of current injected through the electrode. # action potentials (integer values) B. Voltage-current relationship (I-V) Description: The membrane voltage in response to injected current values. mV C. Resting-membrane potential (RMP) Description: The membrane potential without any current injected mV D. Rheobase current (I_rheobase) Description: The amount of current needed to evoke a single action potential pA Number of Rows: 78 Column Names: A. MOUSE_ID: the identifier of the mouse recorded integer B. GENOTYPE: the genotype of the mouse recorded txt C. tdTom+/-: whether the cell recorded was positive or negative for tdTomato expression + (positive), or - (negative) D. I_inj:X: the response to X pA of current injected varies by sheet (i.e., for sheet 'f-I' it corresponds to #action_potentials) E. RMP: resting membrane potential for this cell mV F. I_rheobase: the rheobase recorded for this cell pA Missing data codes: A. NaN: could not extract a value from the recorded trace (i.e., the current injection was so large that the voltage response trace was too large in amplitude to isolate action potentials) ---------------------------------------- DATA SPECIFIC INFORMATION FOR 'SOURCE_DATA_FIG_5.xlsx' ---------------------------------------- Date of Creation: 2025-05-20 Description of Data: Electrophysiological parameters from whole-cell recordings + current injections from striatal D1-FlpO and A2a-FlpO neurons. Number of Variables: 4 Variable List: A. Number of action potentials in response to current injected (f-I) Description: Each recorded neuron's number of action potentials evoked by a set amount of current injected through the electrode. # action potentials (integer values) B. Voltage-current relationship (I-V) Description: The membrane voltage in response to injected current values. mV C. Resting-membrane potential (RMP) Description: The membrane potential without any current injected mV D. Rheobase current (I_rheobase) Description: The amount of current needed to evoke a single action potential pA Number of Rows: 78 Column Names: A. MOUSE_ID: the identifier of the mouse recorded integer B. GENOTYPE: the genotype of the mouse recorded txt C. tdTom+/-: whether the cell recorded was positive or negative for tdTomato expression + (positive), or - (negative) D. I_inj:X: the response to X pA of current injected varies by sheet (i.e., for sheet 'f-I' it corresponds to #action_potentials) E. RMP: resting membrane potential for this cell mV F. I_rheobase: the rheobase recorded for this cell pA Missing data codes: A. NaN: could not extract a value from the recorded trace (i.e., the current injection was so large that the voltage response trace was too large in amplitude to isolate action potentials) ---------------------------------------- DATA SPECIFIC INFORMATION FOR 'SOURCE_DATA_FIG_6.xlsx' ---------------------------------------- Date of Creation: 2025-05-20 Description of Data: Electrophysiological parameters from whole-cell recordings + current injections from midbrain dopamine neurons (DAT-FlpO) Number of Variables: 4 Variable List: A. Number of action potentials in response to current injected (f-I) Description: Each recorded neuron's number of action potentials evoked by a set amount of current injected through the electrode. # action potentials (integer values) B. Voltage-current relationship (I-V) Description: The membrane voltage in response to injected current values. mV C. Input resistance from I-V fit (Ri_fit) Description: The neuron membrane resistance, determined by linear fit of the sub threshold I-V response curve. mega-Ohms D. Input resistance measured (Ri_meas) Description: The neuron membrane resistance, measured/calculated from the test-pulse. mega-Ohms E. Resting-membrane potential (RMP) Description: The membrane potential without any current injected mV F. Rheobase current (I_rheobase) Description: The amount of current needed to evoke a single action potential pA Number of Rows: 15 Column Names: A. MOUSE_ID: the identifier of the mouse recorded integer B. GENOTYPE: the genotype of the mouse recorded txt C. SNc/VTA: whether the cell recorded was from the SNc or VTA SNc or VTA D. I_inj:X: the response to X pA of current injected varies by sheet (i.e., for sheet 'f-I' it corresponds to #action_potentials) E. Ri_fit: input resistance (from fit) mega-Ohm F. Ri_meas: input resistance (from test_pulse) mega-Ohm G. RMP: resting membrane potential for this cell mV H. I_rheobase: the rheobase recorded for this cell pA Missing data codes: A. NaN: could not extract a value from the recorded trace (i.e., the current injection was so large that the voltage response trace was too large in amplitude to isolate action potentials) ----------- METHODOLOGY ----------- Description of methods used for data collection: All recordings were made using a Multiclamp 700B amplifier, the output of which was digitized at 10 kHz (Digidata 1440A). Series resistance was always <35 MΩ and was compensated up to 90%. Neurons were targeted with DIC microscopy and epifluorescence when appropriate. Description of methods used for data processing: All data was processed using custom MATLAB code (see Key Resources Table), with values directly taken from MATLAB output into these Source Data files. ----------------------- DATA ACCESS AND SHARING ----------------------- Publications based on this dataset: Albarran et al. (in preparation) Recommended citation for this dataset: TBD License information: Creative Commons Attribution 4.0 International