A0A2R9YJI3,PROTEIN NAMES: G-protein coupled receptor 22 PROTEIN FAMILY: G-protein coupled receptor 1 family ORGANISM: Danio rerio (Zebrafish) (Brachydanio rerio) SIMILARITY: Belongs to the G-protein coupled receptor 1 family. FUNCTION: Orphan G-protein coupled receptor that regulates cilia length and structure in the Kupffer's vesicle leading to the left-right asymmetry development by establishing a directional fluid flow. KEYWORDS: Cell membrane;Cilium biogenesis/degradation;G-protein coupled receptor;Membrane;Receptor;Reference proteome;Transducer;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein A0A2T5Y4G4,"PROTEIN NAMES: CD-NTase-associated protein 12 (Cap12) (NAD(+) hydrolase) (TIR-STING) (SfSTING) PROTEIN FAMILY: Bacterial STING family ORGANISM: Sphingobacterium faecium (strain DSM 11690 / JCM 21820 / NBRC 15299 / NCIMB 13408 / KS 0470) SIMILARITY: In the C-terminal section; belongs to the bacterial STING family. FUNCTION: Effector protein of a CBASS antiviral system with NAD(+) hydrolase activity. CBASS (cyclic oligonucleotide-based antiphage signaling system) provides immunity against bacteriophage. The CD-NTase protein synthesizes cyclic nucleotides in response to infection; these serve as specific second messenger signals. The signals activate a diverse range of effectors, leading to bacterial cell death and thus abortive phage infection. A type I-D(GG) CBASS system.; FUNCTION: Upon activation by 3'3'-c-di-GMP forms filaments which hydrolyze NAD(+); filament formation is required for enzyme activation. Induction in an E.coli strain that synthesizes c-di-GMP leads to significant growth inhibition. Binds c-di-GMP and 3'3'-cGAMP (3'3'-cyclic GMP-AMP), but not c-di-AMP, 2'3'-cGAMP or cUMP-AMP. KEYWORDS: 3D-structure;Antiviral defense;Hydrolase;Nucleotide-binding " A0A2Y9GHM3,"PROTEIN NAMES: Apolipoprotein E (Apo-E) PROTEIN FAMILY: Apolipoprotein A1/A4/E family ORGANISM: Neomonachus schauinslandi (Hawaiian monk seal) (Monachus schauinslandi) SIMILARITY: Belongs to the apolipoprotein A1/A4/E family. FUNCTION: APOE is an apolipoprotein, a protein associating with lipid particles, that mainly functions in lipoprotein-mediated lipid transport between organs via the plasma and interstitial fluids. APOE is a core component of plasma lipoproteins and is involved in their production, conversion and clearance. Apolipoproteins are amphipathic molecules that interact both with lipids of the lipoprotein particle core and the aqueous environment of the plasma. As such, APOE associates with chylomicrons, chylomicron remnants, very low density lipoproteins (VLDL) and intermediate density lipoproteins (IDL) but shows a preferential binding to high-density lipoproteins (HDL). It also binds a wide range of cellular receptors including the LDL receptor/LDLR, the LDL receptor-related proteins LRP1, LRP2 and LRP8 and the very low-density lipoprotein receptor/VLDLR that mediate the cellular uptake of the APOE-containing lipoprotein particles. Finally, APOE has also a heparin-binding activity and binds heparan-sulfate proteoglycans on the surface of cells, a property that supports the capture and the receptor-mediated uptake of APOE-containing lipoproteins by cells. A main function of APOE is to mediate lipoprotein clearance through the uptake of chylomicrons, VLDLs, and HDLs by hepatocytes. APOE is also involved in the biosynthesis by the liver of VLDLs as well as their uptake by peripheral tissues ensuring the delivery of triglycerides and energy storage in muscle, heart and adipose tissues. By participating in the lipoprotein-mediated distribution of lipids among tissues, APOE plays a critical role in plasma and tissues lipid homeostasis. APOE is also involved in two steps of reverse cholesterol transport, the HDLs-mediated transport of cholesterol from peripheral tissues to the liver, and thereby plays an important role in cholesterol homeostasis. First, it is functionally associated with ABCA1 in the biogenesis of HDLs in tissues. Second, it is enriched in circulating HDLs and mediates their uptake by hepatocytes. APOE also plays an important role in lipid transport in the central nervous system, regulating neuron survival and sprouting. KEYWORDS: Chylomicron;Endosome;Extracellular matrix;Glycoprotein;HDL;Heparin-binding;Lipid-binding;Oxidation;Phosphoprotein;Reference proteome;Repeat;Secreted;Signal;VLDL SUBCELLULAR LOCATION: Secreted Secreted, extracellular space Secreted, extracellular space, extracellular matrix Extracellular vesicle Endosome, multivesicular body Note=In the plasma, APOE is associated with chylomicrons, chylomicrons remnants, VLDL, LDL and HDL lipoproteins. Lipid poor oligomeric APOE is associated with the extracellular matrix in a calcium- and heparan-sulfate proteoglycans-dependent manner. Lipidation induces the release from the extracellular matrix. Colocalizes with CD63 and PMEL at exosomes and in intraluminal vesicles within multivesicular endosomes. " A0A336U966,"PROTEIN NAMES: Tyrosinase P PROTEIN FAMILY: Tyrosinase family ORGANISM: Aspergillus terreus SIMILARITY: Belongs to the tyrosinase family. FUNCTION: Tyrosinase; part of the gene cluster that mediates the biosynthesis of Asp-melanin, a pigment that confers resistance against UV light and hampers phagocytosis by soil amoeba. The nonribosomal peptide synthase melA converts 4-hydroxyphenylpyruvate (4-HPPA) to aspulvinone E. The tyrosinase tyrP then performs hydroxylations of both aromatic moieties of aspulvinone E. The product of tyrP is highly unstable, and, due to the high reactivity of methides and ortho-diquinones, the polymeric Asp-melanin forms spontaneously. KEYWORDS: Copper;Endoplasmic reticulum;Glycoprotein;Golgi apparatus;Metal-binding;Oxidoreductase;Signal SUBCELLULAR LOCATION: Endoplasmic reticulum lumen Golgi apparatus lumen Note=The oxidizing environment of Golgi or endoplasmic reticulum (ER) is required for tyrP to be active. " A0A396JG59,"PROTEIN NAMES: Exocyst complex component EXO70I (MtExo70I) (Exocyst subunit Exo70 family protein I) PROTEIN FAMILY: EXO70 family ORGANISM: Medicago truncatula (Barrel medic) (Medicago tribuloides) SIMILARITY: Belongs to the EXO70 family. FUNCTION: Component of an exocyst subcomplex specifically required for periarbuscular membrane (PAM) biogenesis during arbuscular mycorrhizal (AM) symbiosis with AM fungi (e.g. Glomus versiforme), especially critical during the early branching phase of arbuscule development; probably involved in STR and STR2 delivery into the PAM. KEYWORDS: Cell membrane;Coiled coil;Exocytosis;Glycoprotein;Membrane;Protein transport;Reference proteome;Signal;Transport SUBCELLULAR LOCATION: Cell membrane ; Peripheral membrane protein ; Cytoplasmic side Note=During arbuscule branching, restricted to zones adjacent to the periarbuscular membrane (PAM) around the arbuscule hyphal tips. " A0A3Q2TTB3,"PROTEIN NAMES: DNA-directed primase/polymerase protein PROTEIN FAMILY: Eukaryotic-type primase small subunit family ORGANISM: Gallus gallus (Chicken) SIMILARITY: Belongs to the eukaryotic-type primase small subunit family. FUNCTION: DNA primase and DNA polymerase required to tolerate replication-stalling lesions by bypassing them. Required to facilitate mitochondrial and nuclear replication fork progression by initiating de novo DNA synthesis using dNTPs and acting as an error-prone DNA polymerase able to bypass certain DNA lesions. Shows a high capacity to tolerate DNA damage lesions such as 8oxoG and abasic sites in DNA (By similarity). Provides different translesion synthesis alternatives when DNA replication is stalled: able to synthesize DNA primers downstream of lesions, such as UV lesions, R-loops and G-quadruplexes, to allow DNA replication to continue. Can also realign primers ahead of 'unreadable lesions' such as abasic sites and 6-4 photoproduct (6-4 pyrimidine-pyrimidinone), thereby skipping the lesion. Repriming avoids fork degradation while leading to accumulation of internal ssDNA gaps behind the forks (By similarity). Also able to incorporate nucleotides opposite DNA lesions such as 8oxoG, like a regular translesion synthesis DNA polymerase (By similarity). Also required for reinitiating stalled forks after ultraviolet (UV) damage during nuclear DNA replication (By similarity). Required for mitochondrial DNA (mtDNA) synthesis and replication, by reinitiating synthesis after UV damage or in the presence of chain-terminating nucleotides (By similarity). In addition to its role in DNA damage response, also required to maintain efficient nuclear and mitochondrial DNA replication in unperturbed cells (By similarity). KEYWORDS: Alternative splicing;Chromosome;Coiled coil;DNA damage;DNA repair;DNA-directed DNA polymerase;DNA-directed RNA polymerase;Manganese;Metal-binding;Mitochondrion;Nucleotidyltransferase;Nucleus;Reference proteome;Transcription;Transferase;Zinc SUBCELLULAR LOCATION: Nucleus Mitochondrion matrix Chromosome " A0A3S5YBC7,"PROTEIN NAMES: Endoglycoceramidase I (EGCase I) PROTEIN FAMILY: Glycosyl hydrolase 5 (cellulase A) family ORGANISM: Rhodococcus hoagii (strain 103S) (Rhodococcus equi) SIMILARITY: Belongs to the glycosyl hydrolase 5 (cellulase A) family. FUNCTION: Hydrolyzes glycosphingolipids; exhibits broad substrate specificity including monosialodihexosylganglioside (GM3), monosialotetrahexosylganglioside (GM1), fucosyl-GM1, lactosylceramide, globotriosylceramide, globotetraosylceramide, ganglioside GD1a, and ganglioside GD1b. No activity towards glucosylceramide and galactosylceramide. KEYWORDS: 3D-structure;Disulfide bond;Glycosidase;Hydrolase;Lipid metabolism;Lipoprotein;Membrane;Palmitate;Secreted;Signal;Sphingolipid metabolism SUBCELLULAR LOCATION: Secreted Membrane ; Lipid-anchor " A0A3S7WQS5,PROTEIN NAMES: O-phosphoseryl-tRNA(Sec) selenium transferase (Selenocysteine synthase) (Selenocysteinyl-tRNA(Sec) synthase) (Sep-tRNA:Sec-tRNA synthase) PROTEIN FAMILY: SepSecS family ORGANISM: Leishmania donovani SIMILARITY: Belongs to the SepSecS family. FUNCTION: Converts O-phosphoseryl-tRNA(Sec) to selenocysteinyl-tRNA(Sec) required for selenoprotein biosynthesis. PATHWAY: Aminoacyl-tRNA biosynthesis; selenocysteinyl-tRNA(Sec) biosynthesis; selenocysteinyl-tRNA(Sec) from L-seryl-tRNA(Sec) (archaeal/eukaryal route): step 2/2. KEYWORDS: Cytoplasm;Protein biosynthesis;Pyridoxal phosphate;RNA-binding;Selenium;Transferase;tRNA-binding SUBCELLULAR LOCATION: Cytoplasm A0A5K1K8H0,"PROTEIN NAMES: Calcium-dependent protein kinase 5 (PfCDPK5) PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family, CDPK subfamily ORGANISM: Plasmodium falciparum (isolate 3D7) SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily. FUNCTION: Calcium-dependent protein kinase which acts as a sensor and effector of intracellular Ca(2+) levels probably in part downstream of cGMP-activated PKG kinase. Plays a central role in host erythrocytes and hepatocytes infection cycles. During the liver stage, involved in sporozoite motility and thus in sporozoite invasion of host hepatocytes, probably together with CDPK1 and CDPK4 (By similarity). Involved in merosome egress from host hepatocytes, probably together with CDPK4 (By similarity). Required for the release of hepatic merozoites from merosomes in the host blood stream (By similarity). During the asexual blood stage, required for merozoite egress from host erythrocytes by triggering microneme secretion. Phosphorylates transporter NPT1 at late schizont stage. KEYWORDS: ATP-binding;Calcium;Cell membrane;Cytoplasm;Cytoplasmic vesicle;Kinase;Lipoprotein;Membrane;Metal-binding;Nucleotide-binding;Palmitate;Phosphoprotein;Reference proteome;Repeat;Serine/threonine-protein kinase;Transferase SUBCELLULAR LOCATION: Cytoplasm Cytoplasmic vesicle, secretory vesicle, microneme membrane ; Peripheral membrane protein ; Cytoplasmic side Cell membrane ; Peripheral membrane protein ; Cytoplasmic side Note=During the late stages of schizogony, localizes to the cytoplasm in immature daughter merozoites, co-localizes with AMA1 to a subset of micronemes and to the apical region in maturing daughter merozoites, and near the plasma membrane in mature daughter and free merozoites. " A0FKE6,"PROTEIN NAMES: Threonine dehydratase 1 biosynthetic, chloroplastic (SlTD1) (Threonine deaminase 1) PROTEIN FAMILY: Serine/threonine dehydratase family ORGANISM: Solanum lycopersicum (Tomato) (Lycopersicon esculentum) SIMILARITY: Belongs to the serine/threonine dehydratase family. FUNCTION: Has a housekeeping role in isoleucine biosynthesis (Probable). PATHWAY: Amino-acid biosynthesis; L-isoleucine biosynthesis; 2-oxobutanoate from L-threonine: step 1/1. KEYWORDS: Amino-acid biosynthesis;Branched-chain amino acid biosynthesis;Chloroplast;Isoleucine biosynthesis;Lyase;Plastid;Pyridoxal phosphate;Reference proteome;Repeat;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast " A0JPQ4,"PROTEIN NAMES: Tripartite motif-containing protein 72 (Mitsugumin-53) (Mg53) PROTEIN FAMILY: TRIM/RBCC family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the TRIM/RBCC family. FUNCTION: Muscle-specific protein that plays a central role in cell membrane repair by nucleating the assembly of the repair machinery at injury sites. Specifically binds phosphatidylserine. Acts as a sensor of oxidation: upon membrane damage, entry of extracellular oxidative environment results in disulfide bond formation and homooligomerization at the injury site. This oligomerization acts as a nucleation site for recruitment of TRIM72-containing vesicles to the injury site, leading to membrane patch formation. Probably acts upstream of the Ca(2+)-dependent membrane resealing process. Required for transport of DYSF to sites of cell injury during repair patch formation. Regulates membrane budding and exocytosis. May be involved in the regulation of the mobility of KCNB1-containing endocytic vesicles (By similarity). KEYWORDS: Cell membrane;Coiled coil;Cytoplasmic vesicle;Disulfide bond;Exocytosis;Membrane;Metal-binding;Phosphoprotein;Reference proteome;S-nitrosylation;Transport;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cell membrane, sarcolemma Cytoplasmic vesicle membrane Note=Tethered to plasma membrane and cytoplasmic vesicles via its interaction with phosphatidylserine. " A0LNN5,"PROTEIN NAMES: L-lactate transporter (SfMCT) PROTEIN FAMILY: Major facilitator superfamily, Monocarboxylate porter family ORGANISM: Syntrophobacter fumaroxidans (strain DSM 10017 / MPOB) SIMILARITY: Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family. FUNCTION: Proton-coupled L-lactate specific transporter. KEYWORDS: 3D-structure;Cell inner membrane;Cell membrane;Membrane;Reference proteome;Symport;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell inner membrane ; Multi-pass membrane protein " A0MTQ2,"PROTEIN NAMES: Beta-peptidyl aminopeptidase BapA [Cleaved into: Beta-peptidyl aminopeptidase BapA alpha subunit; Beta-peptidyl aminopeptidase BapA beta subunit] PROTEIN FAMILY: Peptidase S58 family ORGANISM: Sphingosinicella microcystinivorans SIMILARITY: Belongs to the peptidase S58 family. FUNCTION: Beta-aminopeptidase that can cleave synthetic beta-peptides which consist of backbone-elongated beta-amino acid residues that are not processed by common proteolytic enzymes. Can cleave the beta-peptides beta-homoVal-beta-homoAla-beta-homoLeu and beta-homoAla-beta-homoLeu. Requires a beta-amino acid at the N-terminus of peptide substrates and cleaves the peptide bond between the N-terminal beta-amino acid and the amino acid at the second position of tripeptidic substrates of the general structure H-betahXaa-Ile-betahTyr-OH according to the following preferences with regard to the side chain of the N-terminal beta-amino acid: aliphatic and aromatic > OH-containing > hydrogen, basic and polar. beta-homoVal-beta-homoAla-beta-homoLeu and beta-homoAla-beta-homoLeu. KEYWORDS: Aminopeptidase;Hydrolase;Periplasm;Protease;Signal SUBCELLULAR LOCATION: Periplasm MISCELLANEOUS: S.microcystinivorans can degrade microcystin, a cyclic, toxic heptapeptide that contains beta-peptidic substructures." A0PJY2,PROTEIN NAMES: Fez family zinc finger protein 1 (Zinc finger protein 312B) PROTEIN FAMILY: Krueppel C2H2-type zinc-finger protein family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein family. FUNCTION: Transcription repressor. Involved in the axonal projection and proper termination of olfactory sensory neurons (OSN). Plays a role in rostro-caudal patterning of the diencephalon and in prethalamic formation. Expression is required in OSN to cell-autonomously regulate OSN axon projections. Regulates non-cell-autonomously the layer formation of the olfactory bulb development and the interneurons. May be required for correct rostral migration of the interneuron progenitors (By similarity). KEYWORDS: Alternative splicing;Developmental protein;Differentiation;Disease variant;DNA-binding;Hypogonadotropic hypogonadism;Kallmann syndrome;Metal-binding;Neurogenesis;Nucleus;Reference proteome;Repeat;Repressor;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: Triggers oncogenic activity specifically in gastric tumors through activation of KRAS in the ERK signaling pathway. A0QVH8,"PROTEIN NAMES: Zinc metalloprotease Rip1 (Regulator of sigma KLM proteases) (S2P endopeptidase) (Site-2-type intramembrane protease) (site-2 protease Rip1) (S2P protease Rip1) PROTEIN FAMILY: Peptidase M50B family ORGANISM: Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) SIMILARITY: Belongs to the peptidase M50B family. FUNCTION: A probable intramembrane site-2 protease (S2P) that cleaves type-2 transmembrane proteins within their membrane-spanning domains. Degrades PbpB (PBP3, FtsI) under conditions of oxidatives stress; degradation is inhibited by Wag31-PbpB interaction. Also cleaves anti-sigma factors RskA, RslA and RslM. Site-1 proteases have not yet been identified in this organism.; FUNCTION: Regulated intramembrane proteolysis (RIP) occurs when an extracytoplasmic signal (possibly oxidative stress) triggers a concerted proteolytic cascade to transmit information and elicit cellular responses. The membrane-spanning regulatory substrate protein (includes anti-sigma factors RskA, RslA, RsmA, and PbpB) is first cut extracytoplasmically (site-1 protease, S1P), then within the membrane itself (site-2 protease, S2P, this entry), while cytoplasmic proteases finish degrading the regulatory protein, liberating the effector protein (ECF sigma factors SigK, SigL and SigM). KEYWORDS: Cell membrane;Hydrolase;Membrane;Metal-binding;Metalloprotease;Protease;Reference proteome;Transmembrane;Transmembrane helix;Zinc SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein " A0QXD8,"PROTEIN NAMES: Erythritol/L-threitol dehydrogenase PROTEIN FAMILY: Zinc-containing alcohol dehydrogenase family ORGANISM: Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) SIMILARITY: Belongs to the zinc-containing alcohol dehydrogenase family. FUNCTION: Catalyzes the NAD-dependent reversible oxidation of erythritol and L-threitol. Involved in the degradation pathways of erythritol and L-threitol, that allow M.smegmatis to grow on these compounds as the sole carbon source. PATHWAY: Carbohydrate metabolism; erythritol degradation.; PATHWAY: Carbohydrate metabolism; L-threitol degradation. KEYWORDS: Carbohydrate metabolism;Metal-binding;NAD;Oxidoreductase;Reference proteome;Zinc " A0R3F9,PROTEIN NAMES: Acetyltransferase Pat (GCN5-related N-acetyltransferase) (GNAT) (Protein acetyltransferase) (Pat) ORGANISM: Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) FUNCTION: Catalyzes specifically the acetylation of the epsilon-amino group of a highly conserved lysine residue in acetyl-CoA synthetase (ACS) and of the universal stress protein (USP) MSMEG_4207. Acetylation results in the inactivation of ACS activity and could be important for mycobacteria to adjust to environmental changes. KEYWORDS: 3D-structure;Acyltransferase;Magnesium;Metal-binding;Reference proteome;Transferase A0R4M9,"PROTEIN NAMES: Trehalose-6-phosphate synthase (TPS) (Alpha,alpha-trehalose-phosphate synthase [UDP-forming]) (Osmoregulatory trehalose synthesis protein A) (OtsA) PROTEIN FAMILY: Glycosyltransferase 20 family ORGANISM: Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) SIMILARITY: Belongs to the glycosyltransferase 20 family. FUNCTION: Involved in the production of glycogen and alpha-glucan via the TreS-Pep2 branch involved in the biosynthesis of maltose-1-phosphate (M1P), and probably in the osmoprotection via the biosynthesis of trehalose. Catalyzes the transfer of glucose from UDP-glucose (UDP-Glc) to glucose-6-phosphate (Glc-6-P) to form trehalose-6-phosphate (Ref.4). ADP-Glc, CDP-Glc, GDP-Glc and TDP-Glc are also glucosyl donors, however, when the pyrimidine sugar nucleotides (CDP-Glc, TDP-Glc and UDP-Glc) are used as substrates, there is an absolute requirement for a high molecular weight polyanion for activity (Ref.4). PATHWAY: Glycan biosynthesis; trehalose biosynthesis. KEYWORDS: Glycosyltransferase;Reference proteome;Transferase " A0R4Q6,"PROTEIN NAMES: Steroid C26-monooxygenase (Cholest-4-en-3-one C26-monooxygenase) (Cholest-4-en-3-one C26-monooxygenase [(25R)-3-oxocholest-4-en-26-oate forming]) (Cholesterol C26-monooxygenase) (Cholesterol C26-monooxygenase [(25R)-3beta-hydroxycholest-5-en-26-oate forming]) (Cytochrome P450 142) (Steroid C27-monooxygenase) PROTEIN FAMILY: Cytochrome P450 family ORGANISM: Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) SIMILARITY: Belongs to the cytochrome P450 family. FUNCTION: Involved in the utilization of cholesterol as the sole carbon and energy source by degrading the side chain. Primarily catalyzes the sequential oxidation of the terminal methyl of cholest-4-en-3-one into (25R)-26-hydroxycholest-4-en-3-one (alcohol), (25R)-26-oxocholest-4-en-3-one (aldehyde), to finally yield the carboxylic acid (25R)-3-oxocholest-4-en-26-oate. Also able to sequentially oxidize cholesterol itself, not only cholest-4-en-3-one. PATHWAY: Steroid metabolism; cholesterol degradation. KEYWORDS: 3D-structure;Cholesterol metabolism;Heme;Iron;Lipid degradation;Lipid metabolism;Metal-binding;Monooxygenase;NADP;Oxidoreductase;Reference proteome;Steroid metabolism;Sterol metabolism " A0R6D0,"PROTEIN NAMES: Nitroreductase NfnB (NR NfnB) (FMN-dependent NAD(P)H nitroreductase) PROTEIN FAMILY: Nitroreductase family ORGANISM: Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) SIMILARITY: Belongs to the nitroreductase family. FUNCTION: Confers resistance to antitubercular drugs benzothiazinone (BTZ) and dinitrobenzamide (DNB). Inactivates BTZ and DNB by reducing an essential nitro group of these compounds to amino group or to hydroxyl amine, respectively, using NADH or NADPH as source of reducing equivalents; two electrons are transferred. Able to reduce the nitro group of bicyclic nitroimidazole PA-824, but not of quinone menadione, nitrofurazone, methyl-4-nitrobenzoate, 4-nitrobenzene methyl sulfonate or 4-nitroacetophenone. KEYWORDS: 3D-structure;Flavoprotein;FMN;NAD;NADP;Nucleotide-binding;Oxidoreductase;Reference proteome " A0RV30,"PROTEIN NAMES: Fructose-1,6-bisphosphate aldolase/phosphatase (FBP A/P) (FBP aldolase/phosphatase) PROTEIN FAMILY: FBP aldolase/phosphatase family ORGANISM: Cenarchaeum symbiosum (strain A) SIMILARITY: Belongs to the FBP aldolase/phosphatase family. FUNCTION: Catalyzes two subsequent steps in gluconeogenesis: the aldol condensation of dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (GA3P) to fructose-1,6-bisphosphate (FBP), and the dephosphorylation of FBP to fructose-6-phosphate (F6P). PATHWAY: Carbohydrate biosynthesis; gluconeogenesis. KEYWORDS: Carbohydrate metabolism;Gluconeogenesis;Hydrolase;Lyase;Magnesium;Metal-binding;Oxidoreductase;Reference proteome;Schiff base " A1A6H3,"PROTEIN NAMES: Ribokinase (AtRBSK) (RK) PROTEIN FAMILY: Carbohydrate kinase PfkB family, Ribokinase subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the carbohydrate kinase PfkB family. Ribokinase subfamily. FUNCTION: Catalyzes the phosphorylation of ribose at O-5 in a reaction requiring ATP and magnesium. The resulting D-ribose-5-phosphate can then be used either for sythesis of nucleotides, histidine, and tryptophan, or as a component of the pentose phosphate pathway (By similarity). Can also use xylose and fructose as carbohydrate substrates with a low efficiency. Can use GTP, and, to a lower extent, CTP and UTP as alternative phosphoryl donors. PATHWAY: Carbohydrate metabolism; D-ribose degradation; D-ribose 5-phosphate from beta-D-ribopyranose: step 2/2. KEYWORDS: 3D-structure;ATP-binding;Carbohydrate metabolism;Chloroplast;Kinase;Magnesium;Metal-binding;Nucleotide-binding;Plastid;Potassium;Reference proteome;Transferase;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast stroma, chloroplast nucleoid " A1A6M1,"PROTEIN NAMES: Protein disulfide isomerase pTAC5, chloroplastic (Protein PLASTID TRANSCRIPTIONALLY ACTIVE 5) (pTAC5) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Exhibits zinc-dependent disulfide isomerase activity. Required for seedling and chloroplast development under heat stress, probably by maintaining plastid-encoded RNA polymerase (PEP)-dependent transcription. KEYWORDS: Alternative splicing;Chloroplast;Coiled coil;Isomerase;Metal-binding;Plastid;Reference proteome;Stress response;Transcription;Transcription regulation;Transit peptide;Zinc;Zinc-finger SUBCELLULAR LOCATION: Plastid, chloroplast stroma, chloroplast nucleoid " A1TFU9,PROTEIN NAMES: FAD-dependent urate hydroxylase (Flavoprotein urate hydroxylase) PROTEIN FAMILY: FAD-dependent urate hydroxylase family ORGANISM: Mycolicibacterium vanbaalenii (strain DSM 7251 / JCM 13017 / BCRC 16820 / KCTC 9966 / NRRL B-24157 / PYR-1) (Mycobacterium vanbaalenii) SIMILARITY: Belongs to the FAD-dependent urate hydroxylase family. FUNCTION: Catalyzes the hydroxylation of urate to 5-hydroxyisourate (HIU). Is likely to be involved in the urate degradation pathway to allantoin. Prefers NADH over NADPH as the electron donor. PATHWAY: Purine metabolism; urate degradation. KEYWORDS: FAD;Flavoprotein;Monooxygenase;NAD;Oxidoreductase;Purine metabolism;Reference proteome A1XWY7,"PROTEIN NAMES: Coniferyl alcohol acyltransferase (PhCFAT) ((E)-cinnamyl alcohol acyltransferase) ((E)-sinapoyl alcohol acyltransferase) (Geraniol acyltransferase) (Octan-1-ol acyltransferase) PROTEIN FAMILY: Plant acyltransferase family ORGANISM: Petunia hybrida (Petunia) SIMILARITY: Belongs to the plant acyltransferase family. FUNCTION: Acyltransferase involved in the biosynthesis of the floral volatile isoeugenol, and which promotes the formation of phenylacetaldehyde, phenylethyl alcohol, phenyl-ethyl acetate, phenylethyl benzoate and benzyl acetate. Catalyzes the acetylation of coniferyl alcohol to produce coniferyl acetate. Also active toward 1-octanol, cinnamyl alcohol, geraniol and sinapyl alcohol. PATHWAY: Aromatic compound metabolism; phenylpropanoid biosynthesis. KEYWORDS: Acyltransferase;Phenylpropanoid metabolism;Transferase " A1Z6J5,PROTEIN NAMES: Tubulin-specific chaperone E (Tubulin-folding cofactor E) PROTEIN FAMILY: TBCE family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the TBCE family. FUNCTION: Tubulin-folding protein which is required for the development of the neuronal microtubule network. Essential for the development and function of neuromuscular synapses. Likely to promote microtubule formation by acting in the negative regulation of the microtubule-severing protein spas. KEYWORDS: Chaperone;Cytoplasm;Leucine-rich repeat;Neurogenesis;Reference proteome;Repeat SUBCELLULAR LOCATION: Cytoplasm A1Z9G2,"PROTEIN NAMES: Pre-mRNA-splicing factor syf1 homolog (Pre-mRNA-splicing factor fandango) (Protein faint sausage) PROTEIN FAMILY: Crooked-neck family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the crooked-neck family. FUNCTION: Subunit of the NTC(Nineteen)/Prp19 complex, which is part of the spliceosome. The complex participates in spliceosome assembly, its remodeling and is required for efficient spliceosome activation. Essential for efficient pre-mRNA splicing. In embryos, efficient pre-mRNA splicing of zygotic transcripts is essential during dynamic cellular processes that require rapid division and/or dramatic changes in gene expression such as blastoderm cellularization, tracheal branching morphogenesis, Malpighian morphogenesis and epidermal development. Part of its role in promoting embryo tracheal development is also due to specifically splicing bnl transcripts which results in the activation of the BNL-FGF pathway. KEYWORDS: mRNA processing;mRNA splicing;Nucleus;Reference proteome;Repeat;Spliceosome SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: Named 'fandango' after the Iberian folk dance to highlight the similarity of the blastoderm cellularization phenotype to the charleston/kuk phenotype. The phenotype 'faint sausage (fas)', consisting of a poorly differentiated cuticle and head, was previously associated with the immunoglobulin domain protein CG17716 but is now known to be caused by mutations in the protein 'fandango'." A2A6Q5,"PROTEIN NAMES: Cell division cycle protein 27 homolog PROTEIN FAMILY: APC3/CDC27 family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the APC3/CDC27 family. FUNCTION: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (By similarity). PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Alternative splicing;Cytoplasm;Cytoskeleton;Nucleus;Phosphoprotein;Reference proteome;Repeat;TPR repeat;Ubl conjugation pathway SUBCELLULAR LOCATION: Nucleus Cytoplasm, cytoskeleton, spindle " A2A825,"PROTEIN NAMES: Ciliogenesis and planar polarity effector 2 (REM2- and Rab-like small GTPase 1) PROTEIN FAMILY: Small GTPase superfamily, Rab family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the small GTPase superfamily. Rab family. FUNCTION: Potential effector of the planar cell polarity signaling pathway. Plays a role in targeted membrane trafficking most probably at the level of vesicle fusion with membranes. Involved in cilium biogenesis by regulating the transport of cargo proteins to the basal body and to the apical tips of cilia. More generally involved in exocytosis in secretory cells (By similarity). KEYWORDS: 3D-structure;Cell projection;Cilium;Cilium biogenesis/degradation;Cytoplasm;Cytoskeleton;Exocytosis;GTP-binding;Nucleotide-binding;Protein transport;Reference proteome;Transport SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, cilium basal body " A2AC93,"PROTEIN NAMES: Dynein axonemal intermediate chain 2 (Axonemal dynein intermediate chain 2) PROTEIN FAMILY: Dynein intermediate chain family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the dynein intermediate chain family. FUNCTION: Part of the dynein complex of respiratory cilia. KEYWORDS: Alternative splicing;Cell projection;Cilium;Cilium biogenesis/degradation;Cytoplasm;Cytoskeleton;Dynein;Microtubule;Motor protein;Reference proteome;Repeat;WD repeat SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, cilium axoneme Dynein axonemal particle Note=Located in the proximal region of respiratory cilia. " A2AG06,PROTEIN NAMES: Meiosis-specific coiled-coil domain-containing protein MEIOC (Meiosis-specific with coiled-coil domain protein) ORGANISM: Mus musculus (Mouse) FUNCTION: Is required for meiosis completion in both male and female germ cells. Confers stability to numerous meiotic mRNAs in gonads allowing proper initiation and progression into meiosis prophase I. The function may involve YTHDC2 and is independent of induction by retinoic acid (RA). Maintains an extended meiotic prophase I by properly promoting the transition from a mitotic to a meiotic cell cycle program by binding transcripts through its interaction with YTHDC2 that regulate the mitotic cell cycle. KEYWORDS: Alternative splicing;Cytoplasm;Meiosis;Nucleus;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Nucleus Note=at late pachytene a fraction is nuclear. A2AJB7,"PROTEIN NAMES: Epididymal-specific lipocalin-5 (Epididymal retinoic acid-binding protein) (E-RABP) (mE-RABP) (Epididymal secretory protein 10) (MEP 10) [Cleaved into: Epididymal-specific lipocalin-5, major form; Epididymal-specific lipocalin-5, minor form] PROTEIN FAMILY: Calycin superfamily, Lipocalin family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the calycin superfamily. Lipocalin family. FUNCTION: Associates with spermatozoa in the epididymal fluid but does not bind tightly to them. Binds both all-trans and 13-cis retinoic acid. May act as a retinoid carrier protein which is required for epididymal function and/or sperm maturation. KEYWORDS: Alternative splicing;Direct protein sequencing;Disulfide bond;Reference proteome;Secreted;Signal;Transport SUBCELLULAR LOCATION: Secreted Note=Synthesized by the mid and distal caput of the epididymis and secreted into the epididymal lumen. " A2ANU3,"PROTEIN NAMES: Synapse differentiation-inducing gene protein 1 (SynDIG1) (Dispanin subfamily C member 2) (DSPC2) (Transmembrane protein 90B) PROTEIN FAMILY: CD225/Dispanin family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the CD225/Dispanin family. FUNCTION: May regulate AMPA receptor content at nascent synapses, and have a role in postsynaptic development and maturation. KEYWORDS: Cell membrane;Cell projection;Endosome;Membrane;Phosphoprotein;Postsynaptic cell membrane;Reference proteome;Signal-anchor;Synapse;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type II membrane protein Early endosome membrane ; Single-pass type II membrane protein Postsynaptic density membrane Synapse Cell projection, dendrite Cell projection, dendritic spine Note=Shuttles between the cell surface and early endosome membrane. " A2APC3,"PROTEIN NAMES: Probable tubulin polyglutamylase TTLL9 (Tubulin--tyrosine ligase-like protein 9) PROTEIN FAMILY: Tubulin--tyrosine ligase family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the tubulin--tyrosine ligase family. FUNCTION: Probable tubulin polyglutamylase that generates side chains of glutamate on the gamma-carboxyl group of specific glutamate residues within the C-terminal tail of target proteins. Similar to TTLL1, may acquire enzymatic activity only in complex with other proteins as it is most likely lacking domains important for autonomous activity (Probable). Mediates tubulin polyglutamylation which induces establishment of microtubule heterogeneity in sperm flagella, thereby playing a role in normal motile flagella axoneme structure and sperm flagella beating pattern. KEYWORDS: Alternative splicing;ATP-binding;Cell projection;Cilium;Cytoplasm;Cytoskeleton;Flagellum;Ligase;Magnesium;Metal-binding;Microtubule;Nucleotide-binding;Reference proteome SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, cilium basal body Cytoplasm, cytoskeleton Cytoplasm, cytoskeleton, flagellum axoneme " A2AQ19,"PROTEIN NAMES: RNA polymerase-associated protein RTF1 homolog ORGANISM: Mus musculus (Mouse) FUNCTION: Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non-phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both independently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. Binds single-stranded DNA (By similarity). Required for maximal induction of heat-shock genes. Required for the trimethylation of histone H3 'Lys-4' (H3K4me3) on genes involved in stem cell pluripotency; this function is synergistic with CXXC1 indicative for an involvement of a SET1 complex. KEYWORDS: Activator;Coiled coil;DNA-binding;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation;Wnt signaling pathway SUBCELLULAR LOCATION: Nucleus, nucleoplasm " A2ARP9,"PROTEIN NAMES: Cation channel sperm-associated protein 2 (CatSper2) PROTEIN FAMILY: Cation channel sperm-associated family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the cation channel sperm-associated (TC 1.A.1.19) family. FUNCTION: Pore-forming subunit of the CatSper complex, a sperm-specific voltage-gated calcium channel that plays a central role in sperm cell hyperactivation. Controls calcium entry to mediate the hyperactivated motility, a step needed for sperm motility which is essential late in the preparation of sperm for fertilization. KEYWORDS: 3D-structure;Calcium;Calcium channel;Calcium transport;Cell membrane;Cell projection;Cilium;Developmental protein;Differentiation;Flagellum;Ion channel;Ion transport;Membrane;Reference proteome;Spermatogenesis;Transmembrane;Transmembrane helix;Transport;Voltage-gated channel SUBCELLULAR LOCATION: Cell projection, cilium, flagellum membrane ; Multi-pass membrane protein " A2AS89,"PROTEIN NAMES: Guanidino acid hydrolase, mitochondrial (Arginase, mitochondrial) (Guanidinobutyrase, mitochondrial) (Guanidinopropionase, mitochondrial) PROTEIN FAMILY: Arginase family, Agmatinase subfamily ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the arginase family. Agmatinase subfamily. FUNCTION: Hydrolyzes linear guanidino acids to form urea and the corresponding amines. Displays specificity for substrates having a negatively charged head group and short chains including taurocyamine, guanidino propanoic and butanoic acids. May protect cells by detoxifying potentially harmful amounts of guanidino acids. Metabolizes L-arginine with low efficiency. PATHWAY: Nitrogen metabolism; urea cycle; L-ornithine and urea from L-arginine: step 1/1. KEYWORDS: Acetylation;Arginine metabolism;Hydrolase;Manganese;Metal-binding;Mitochondrion;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Mitochondrion " A2AU72,"PROTEIN NAMES: Armadillo repeat-containing protein 3 ORGANISM: Mus musculus (Mouse) FUNCTION: Essential for male fertility and sperm motility. During spermatogenesis, promotes the autophagic degradation of excessive ribosomes, providing energy resources for mitochondria and thus ensuring sperm flagellar motility. KEYWORDS: Alternative splicing;Autophagy;Differentiation;Lipoprotein;Palmitate;Reference proteome;Repeat;Spermatogenesis " A2AVZ9,"PROTEIN NAMES: Equilibrative nucleobase transporter 1 (Embryonic epithelia gene 1 protein) (Solute carrier family 43 member 3) PROTEIN FAMILY: SLC43A transporter family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the SLC43A transporter (TC 2.A.1.44) family. FUNCTION: Sodium-independent purine-selective nucleobase transporter which mediates the equilibrative transport of extracellular purine nucleobases such as adenine, guanine and hypoxanthine (By similarity). May regulate fatty acid (FA) transport in adipocytes, acting as a positive regulator of FA efflux and as a negative regulator of FA uptake. KEYWORDS: Alternative splicing;Cell membrane;Glycoprotein;Lipid transport;Membrane;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Basolateral cell membrane ; Multi-pass membrane protein " A2CI35,"PROTEIN NAMES: Dual serine/threonine and tyrosine protein kinase (Dusty protein kinase) (Dusty PK) (Receptor-interacting serine/threonine-protein kinase 5) PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family ORGANISM: Strongylocentrotus purpuratus (Purple sea urchin) SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. FUNCTION: May act as a positive regulator of ERK phosphorylation downstream of fibroblast growth factor-receptor activation. May induce both caspase-dependent apoptosis and caspase-independent cell death. May play a role in the embryonic development. KEYWORDS: ATP-binding;Cell junction;Cell membrane;Cytoplasm;Developmental protein;Kinase;Membrane;Nucleotide-binding;Reference proteome;Serine/threonine-protein kinase;Transferase;Tyrosine-protein kinase SUBCELLULAR LOCATION: Cytoplasm Cell membrane Apical cell membrane Basolateral cell membrane Cell junction " A2QHE5,"PROTEIN NAMES: Ferulic acid decarboxylase 1 (Phenacrylate decarboxylase) PROTEIN FAMILY: UbiD family, UbiD-like/FDC subfamily ORGANISM: Aspergillus niger (strain ATCC MYA-4892 / CBS 513.88 / FGSC A1513) SIMILARITY: Belongs to the UbiD family. UbiD-like/FDC subfamily. FUNCTION: Catalyzes the reversible decarboxylation of aromatic carboxylic acids like ferulic acid, p-coumaric acid or cinnamic acid, producing the corresponding vinyl derivatives 4-vinylphenol, 4-vinylguaiacol, and styrene, respectively, which play the role of aroma metabolites. KEYWORDS: 3D-structure;Cytoplasm;Decarboxylase;Flavoprotein;FMN;Lyase;Manganese;Metal-binding;Reference proteome SUBCELLULAR LOCATION: Cytoplasm " A2QQ28,PROTEIN NAMES: Probable E3 ubiquitin-protein ligase hulA (HECT ubiquitin ligase A) (HECT-type E3 ubiquitin transferase hulA) PROTEIN FAMILY: RSP5/NEDD4 family ORGANISM: Aspergillus niger (strain ATCC MYA-4892 / CBS 513.88 / FGSC A1513) SIMILARITY: Belongs to the RSP5/NEDD4 family. FUNCTION: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Probably involved in the regulatory network controlling carbon source utilization. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Cytoplasm;Reference proteome;Repeat;Transferase;Ubl conjugation pathway SUBCELLULAR LOCATION: Cytoplasm A2RST1,"PROTEIN NAMES: Microsomal glutathione S-transferase 2 (Microsomal GST-2) (Glutathione peroxidase MGST2) (Leukotriene C4 synthase MGST2) (Microsomal glutathione S-transferase II) (Microsomal GST-II) ORGANISM: Mus musculus (Mouse) FUNCTION: Catalyzes several different glutathione-dependent reactions. Catalyzes the glutathione-dependent reduction of lipid hydroperoxides, such as 5-HPETE. Has glutathione transferase activity, toward xenobiotic electrophiles, such as 1-chloro-2, 4-dinitrobenzene (CDNB). Catalyzes also the conjugation of leukotriene A4 with reduced glutathione to form leukotriene C4 (LTC4) (By similarity). Involved in oxidative DNA damage induced by ER stress and anticancer agents by activating LTC4 biosynthetic machinery in nonimmune cells. KEYWORDS: Endoplasmic reticulum;Leukotriene biosynthesis;Lipid metabolism;Lyase;Membrane;Microsome;Oxidoreductase;Reference proteome;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein Microsome membrane ; Multi-pass membrane protein " A2RVM0,"PROTEIN NAMES: Short-chain dehydrogenase TIC 32, chloroplastic (Translocon at the inner envelope membrane of chloroplasts 32) (AtTIC32) PROTEIN FAMILY: Short-chain dehydrogenases/reductases (SDR) family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FUNCTION: Involved in protein precursor import into chloroplasts. Part of the redox regulon consisting of TIC32, TIC 55 and TIC62. KEYWORDS: Alternative splicing;Chloroplast;Membrane;Oxidoreductase;Plastid;Plastid inner membrane;Protein transport;Reference proteome;Transport SUBCELLULAR LOCATION: Plastid, chloroplast inner membrane " A2RVU1,"PROTEIN NAMES: Protein MODIFYING WALL LIGNIN-1 (MWL-1) PROTEIN FAMILY: DESIGUAL family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the DESIGUAL family. FUNCTION: Together with MWL2, contributes to secondary cell wall biology, specifically lignin biosynthesis. KEYWORDS: Alternative splicing;Cell membrane;Lignin biosynthesis;Membrane;Reference proteome;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein " A2T2X4,"PROTEIN NAMES: Intraflagellar transport protein 46 (Flagellar-associated protein 32) (IFT complex B protein) PROTEIN FAMILY: IFT46 family ORGANISM: Chlamydomonas reinhardtii (Chlamydomonas smithii) SIMILARITY: Belongs to the IFT46 family. FUNCTION: Forms part of a complex involved in intraflagellar transport (IFT), the bi-directional movement of particles required for the assembly, maintenance and functioning of primary cilia. Plays a role in maintaining IFT complex B stability. KEYWORDS: 3D-structure;Cell projection;Cilium;Cytoplasm;Cytoskeleton SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, cilium basal body Cell projection, cilium Note=Expression is concentrated at the cilium basal body but is also detected along the length of the cilium. " A2TJ54,"PROTEIN NAMES: Bis(monoacylglycero)phosphate synthase CLN5 (BMP synthase CLN5) (Ceroid-lipofuscinosis neuronal protein 5) (Protein CLN5) (Palmitoyl protein thioesterase CLN5) (S-depalmitoylase CLN5) [Cleaved into: Bis(monoacylglycero)phosphate synthase CLN5, secreted form] PROTEIN FAMILY: CLN5 family ORGANISM: Ovis aries (Sheep) SIMILARITY: Belongs to the CLN5 family. FUNCTION: [Bis(monoacylglycero)phosphate synthase CLN5, secreted form]: Catalyzes the synthesis of bis(monoacylglycero)phosphate (BMP) via transacylation of 2 molecules of lysophosphatidylglycerol (LPG). BMP also known as lysobisphosphatidic acid plays a key role in the formation of intraluminal vesicles and in maintaining intracellular cholesterol homeostasis. Can use only LPG as the exclusive lysophospholipid acyl donor for base exchange and displays BMP synthase activity towards various LPGs (LPG 14:0, LPG 16:0, LPG 18:0, LPG 18:1) with a higher preference for longer chain lengths. Plays a role in influencing the retrograde trafficking of lysosomal sorting receptors SORT1 and IGF2R from the endosomes to the trans-Golgi network by controlling the recruitment of retromer complex to the endosomal membrane. Regulates the localization and activation of RAB7A which is required to recruit the retromer complex to the endosomal membrane.; FUNCTION: Exhibits palmitoyl protein thioesterase (S-depalmitoylation) activity in vitro and most likely plays a role in protein S-depalmitoylation. KEYWORDS: Disulfide bond;Glycoprotein;Hydrolase;Lysosome;Membrane;Neurodegeneration;Neuronal ceroid lipofuscinosis;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: [Bis(monoacylglycero)phosphate synthase CLN5, secreted form]: Lysosome.; SUBCELLULAR LOCATION: [Bis(monoacylglycero)phosphate synthase CLN5]: Membrane ; Single-pass type II membrane protein Note=An amphipathic anchor region facilitates its association with the membrane. " A2VEY9,"PROTEIN NAMES: Palmitoyltransferase app (Protein approximated) PROTEIN FAMILY: DHHC palmitoyltransferase family, ERF2/ZDHHC9 subfamily ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the DHHC palmitoyltransferase family. ERF2/ZDHHC9 subfamily. FUNCTION: Palmitoylates Dlish which is required for the apical cell cortex localization, total cellular level and full activity of dachs. KEYWORDS: Acyltransferase;Alternative splicing;Cell membrane;Endoplasmic reticulum;Lipoprotein;Membrane;Palmitate;Reference proteome;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein Apical cell membrane ; Multi-pass membrane protein " A2XW02,"PROTEIN NAMES: Salt tolerance receptor-like cytoplasmic kinase 1 (Receptor-like cytoplasmic kinase 154) (OsRLCK154) PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family ORGANISM: Oryza sativa subsp. indica (Rice) SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. FUNCTION: Acts probably as a dual specificity protein kinase (Probable). Regulates hydrogen peroxide (H(2)O(2)) homeostasis and improves salt tolerance by phosphorylating tyrosine residues of CATC thus activating its catalase activity. Promotes growth at the seedling stage and prevents grain yield loss under salt stress conditions (By similarity). KEYWORDS: ATP-binding;Cell membrane;Kinase;Lipoprotein;Membrane;Nucleotide-binding;Palmitate;Phosphoprotein;Reference proteome;Serine/threonine-protein kinase;Stress response;Transferase;Tyrosine-protein kinase SUBCELLULAR LOCATION: Cell membrane ; Lipid-anchor " A3MUY9,"PROTEIN NAMES: NAD(+)-dependent glutamate dehydrogenase (NAD-GDH) (NAD-specific glutamate dehydrogenase) PROTEIN FAMILY: Glu/Leu/Phe/Val dehydrogenases family ORGANISM: Pyrobaculum calidifontis (strain DSM 21063 / JCM 11548 / VA1) SIMILARITY: Belongs to the Glu/Leu/Phe/Val dehydrogenases family. FUNCTION: Catalyzes the reversible oxidative deamination of L-glutamate to 2-oxoglutarate and ammonia, thereby playing a key role at the intersection of the carbon and nitrogen metabolic pathways. Is strictly specific for NAD(+)/NADH as the acceptor/donor, since it cannot use NADP(+)/NADPH. May function in vivo in the catabolic direction. Also catalyzes at low rates the oxidative deamination of L-norvaline, L-2-aminobutyrate, L-valine and L-isoleucine, and the reductive amination of 2-oxovalerate and 2-oxobutyrate. KEYWORDS: NAD;Oxidoreductase " A3R064,"PROTEIN NAMES: Docking protein 3 (Downstream of tyrosine kinase 3) PROTEIN FAMILY: DOK family, Type A subfamily ORGANISM: Gallus gallus (Chicken) SIMILARITY: Belongs to the DOK family. Type A subfamily. FUNCTION: DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. Plays a role as negative regulator of the mobilization of calcium ions and of calcium signaling. KEYWORDS: Cell membrane;Cytoplasm;Membrane;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Cell membrane ; Peripheral membrane protein ; Cytoplasmic side " A4D2B8,PROTEIN NAMES: Putative postmeiotic segregation increased 2-like protein 1 (PMS2-related protein 2) (Postmeiotic segregation increased 2-like protein 13) (Postmeiotic segregation increased 2-like protein 6) (Postmeiotic segregation increased 2-like protein 8) (Postmeiotic segregation increased protein 3) (hPMS3) (Postmeiotic segregation increased protein 8) (Putative postmeiotic segregation increased 2 pseudogene 1) PROTEIN FAMILY: DNA mismatch repair MutL/HexB family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the DNA mismatch repair MutL/HexB family. KEYWORDS: Alternative splicing;Kinase;Reference proteome;Transferase MISCELLANEOUS: Encoded by one of the numerous copies of postmeiotic segregation increased 2-like genes scattered in the q11-q22 region of the chromosome 7. A4GXA9,PROTEIN NAMES: Probable crossover junction endonuclease EME2 PROTEIN FAMILY: EME1/MMS4 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the EME1/MMS4 family. FUNCTION: Interacts with MUS81 to form a DNA structure-specific endonuclease which cleaves substrates such as 3'-flap structures. KEYWORDS: 3D-structure;Alternative splicing;DNA damage;DNA recombination;DNA repair;Endonuclease;Hydrolase;Nuclease;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus A4Q9F0,"PROTEIN NAMES: Tubulin polyglutamylase TTLL7 (Tubulin--tyrosine ligase-like protein 7) (mTTLL7) PROTEIN FAMILY: Tubulin--tyrosine ligase family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the tubulin--tyrosine ligase family. FUNCTION: Polyglutamylase which modifies tubulin, generating polyglutamate side chains of variable lengths on the gamma-carboxyl group of specific glutamate residues within the C-terminal tail of tubulin. Mediates both ATP-dependent initiation and elongation steps of the polyglutamylation reaction. Preferentially modifies the beta-tubulin tail over an alpha-tail. Competes with monoglycylase TTLL3 for modification site on beta-tubulin substrate, thereby creating an anticorrelation between glycylation and glutamylation reactions (By similarity). Required for neurite growth; responsible for the strong increase in tubulin polyglutamylation during postnatal neuronal maturation. KEYWORDS: Alternative splicing;ATP-binding;Cell projection;Cilium;Cytoplasm;Cytoskeleton;Developmental protein;Differentiation;Ligase;Magnesium;Metal-binding;Microtubule;Neurogenesis;Nucleotide-binding;Reference proteome SUBCELLULAR LOCATION: Cell projection, cilium Cytoplasm, cytoskeleton, cilium basal body Cell projection, dendrite Perikaryon Note=In cells with primary cilia, found in both cilia and basal bodies. In neuronal cells, found in dendrites and perikaryon. MISCELLANEOUS: [Isoform 2]: May be due to an intron retention." A4Q9F3,"PROTEIN NAMES: Protein polyglycylase TTLL10 (Tubulin--tyrosine ligase-like protein 10) ORGANISM: Mus musculus (Mouse) FUNCTION: Polyglycylase which modifies both tubulin and non-tubulin proteins, generating polyglycine side chains of variable lengths on the gamma-carboxyl groups of specific glutamate residues of target proteins. Involved in the elongation step rather than the initiation step of the polyglycylation reaction. Polyglycylates alpha-tubulin and beta-tubulin. Polyglycylates non-tubulin proteins such as nucleosome assembly protein NAP1. KEYWORDS: Alternative splicing;ATP-binding;Cell projection;Cilium;Cytoplasm;Cytoskeleton;Ligase;Magnesium;Metal-binding;Microtubule;Nucleotide-binding;Reference proteome SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton Cell projection, cilium Cytoplasm, cytoskeleton, cilium axoneme " A4VCL2,PROTEIN NAMES: Extracellular serine/threonine protein CG31145 (Golgi casein kinase) (Golgi-enriched fraction casein kinase) (GEF-CK) PROTEIN FAMILY: FAM20 family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the FAM20 family. FUNCTION: Golgi serine/threonine protein kinase that phosphorylates secretory pathway proteins within Ser-x-Glu/pSer motifs. KEYWORDS: Alternative splicing;ATP-binding;Disulfide bond;Glycoprotein;Golgi apparatus;Kinase;Manganese;Membrane;Metal-binding;Nucleotide-binding;Reference proteome;Secreted;Serine/threonine-protein kinase;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus membrane ; Single-pass type II membrane protein Secreted Note=Resides in the Golgi apparatus membrane and is secreted following propeptide cleavage. A4VFY3,"PROTEIN NAMES: Aspartate kinase Ask_Ect (Aspartokinase) PROTEIN FAMILY: Aspartokinase family ORGANISM: Stutzerimonas stutzeri (strain A1501) (Pseudomonas stutzeri) SIMILARITY: Belongs to the aspartokinase family. FUNCTION: Involved in the biosynthesis of L-aspartate-beta-semialdehyde, which is an intermediate in the biosynthesis of ectoine, a highly soluble organic osmolyte, called compatible solute. Ectoine is used to avoid excessive water efflux, plasmolysis, molecular crowding of the cytoplasm, and cessation of growth in high salinity environments. Catalyzes the phosphorylation of the beta-carboxyl group of L-aspartate to yield 4-phospho-L-aspartate. PATHWAY: Amine and polyamine biosynthesis; ectoine biosynthesis. KEYWORDS: Allosteric enzyme;ATP-binding;Cytoplasm;Kinase;Nucleotide-binding;Reference proteome;Transferase SUBCELLULAR LOCATION: Cytoplasm " A4YDT1,"PROTEIN NAMES: 4-hydroxybutyrate--CoA ligase 1 (Acetate--CoA ligase) (Butyrate--CoA ligase) (Propionate--CoA ligase) PROTEIN FAMILY: ATP-dependent AMP-binding enzyme family ORGANISM: Metallosphaera sedula (strain ATCC 51363 / DSM 5348 / JCM 9185 / NBRC 15509 / TH2) SIMILARITY: Belongs to the ATP-dependent AMP-binding enzyme family. FUNCTION: Involved in the 3-hydroxypropionate/4-hydroxybutyrate cycle which incorporates carbon dioxide into cellular carbon. Catalyzes the ligation of coenzyme A (CoA) to 4-hydroxybutyrate (4HB). It can also use butyrate, valerate, propionate, acetate and 3-hydroxybutyrate (3HB) as substrates. KEYWORDS: 3D-structure;ATP-binding;Fatty acid metabolism;Ligase;Lipid metabolism;Magnesium;Membrane;Nucleotide-binding;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Single-pass membrane protein " A5A6M2,"PROTEIN NAMES: Annexin A1 (Annexin-1) [Cleaved into: Annexin Ac2-26] PROTEIN FAMILY: Annexin family ORGANISM: Pan troglodytes (Chimpanzee) SIMILARITY: Belongs to the annexin family. FUNCTION: Plays important roles in the innate immune response as effector of glucocorticoid-mediated responses and regulator of the inflammatory process. Has anti-inflammatory activity. Plays a role in glucocorticoid-mediated down-regulation of the early phase of the inflammatory response. Contributes to the adaptive immune response by enhancing signaling cascades that are triggered by T-cell activation, regulates differentiation and proliferation of activated T-cells. Promotes the differentiation of T-cells into Th1 cells and negatively regulates differentiation into Th2 cells (By similarity). Has no effect on unstimulated T-cells. Negatively regulates hormone exocytosis via activation of the formyl peptide receptors and reorganization of the actin cytoskeleton (By similarity). Has high affinity for Ca(2+) and can bind up to eight Ca(2+) ions (By similarity). Displays Ca(2+)-dependent binding to phospholipid membranes (By similarity). Plays a role in the formation of phagocytic cups and phagosomes. Plays a role in phagocytosis by mediating the Ca(2+)-dependent interaction between phagosomes and the actin cytoskeleton (By similarity).; FUNCTION: [Annexin Ac2-26]: Functions at least in part by activating the formyl peptide receptors and downstream signaling cascades. Promotes chemotaxis of granulocytes and monocytes via activation of the formyl peptide receptors. Promotes rearrangement of the actin cytoskeleton, cell polarization and cell migration. Promotes resolution of inflammation and wound healing. Acts via neutrophil N-formyl peptide receptors to enhance the release of CXCL2. KEYWORDS: Acetylation;Adaptive immunity;Annexin;Calcium;Calcium/phospholipid-binding;Cell membrane;Cell projection;Cilium;Cytoplasm;Cytoplasmic vesicle;Disulfide bond;Endosome;Immunity;Inflammatory response;Innate immunity;Isopeptide bond;Membrane;Metal-binding;Nucleus;Phospholipase A2 inhibitor;Phosphoprotein;Reference proteome;Repeat;Secreted;Ubl conjugation SUBCELLULAR LOCATION: Nucleus Cytoplasm Cell projection, cilium Basolateral cell membrane Lateral cell membrane Cell membrane ; Peripheral membrane protein Apical cell membrane Membrane ; Peripheral membrane protein Early endosome Cytoplasmic vesicle membrane ; Peripheral membrane protein Endosome membrane ; Peripheral membrane protein Secreted Secreted, extracellular space Cell membrane ; Peripheral membrane protein ; Extracellular side Secreted, extracellular exosome Cytoplasmic vesicle, secretory vesicle lumen Cell projection, phagocytic cup Note=Colocalizes with actin fibers at phagocytic cups. Secreted, at least in part via exosomes and other secretory vesicles. Detected in exosomes and other extracellular vesicles. Secretion is increased in response to wounding and inflammation (By similarity). Alternatively, the secretion is dependent on protein unfolding and facilitated by the cargo receptor TMED10; it results in the protein translocation from the cytoplasm into ERGIC (endoplasmic reticulum-Golgi intermediate compartment) followed by vesicle entry and secretion (By similarity). Detected in gelatinase granules in resting neutrophils. Neutrophil adhesion to endothelial cells stimulates secretion via gelatinase granules, but foreign particle phagocytosis has no effect. Displays calcium-dependent binding to phospholipid membranes (By similarity). MISCELLANEOUS: Was originally identified as calcium and phospholipid binding protein that displays Ca(2+)-dependent binding to phospholipid membranes and can promote membrane aggregation in vitro. Was initially identified as inhibitor of phospholipase A2 activity (in vitro). Inhibition of phospholipase activity is mediated via its phospholipid binding activity that limits the access of phospholipase to its substrates." A5IQA5,"PROTEIN NAMES: Protein/nucleic acid deglycase HchA (Maillard deglycase) PROTEIN FAMILY: Peptidase C56 family, HchA subfamily ORGANISM: Staphylococcus aureus (strain JH9) SIMILARITY: Belongs to the peptidase C56 family. HchA subfamily. FUNCTION: Protein and nucleotide deglycase that catalyzes the deglycation of the Maillard adducts formed between amino groups of proteins or nucleotides and reactive carbonyl groups of glyoxals. Thus, functions as a protein deglycase that repairs methylglyoxal- and glyoxal-glycated proteins, and releases repaired proteins and lactate or glycolate, respectively. Deglycates cysteine, arginine and lysine residues in proteins, and thus reactivates these proteins by reversing glycation by glyoxals. Acts on early glycation intermediates (hemithioacetals and aminocarbinols), preventing the formation of Schiff bases and advanced glycation endproducts (AGE). Also functions as a nucleotide deglycase able to repair glycated guanine in the free nucleotide pool (GTP, GDP, GMP, dGTP) and in DNA and RNA. Is thus involved in a major nucleotide repair system named guanine glycation repair (GG repair), dedicated to reversing methylglyoxal and glyoxal damage via nucleotide sanitization and direct nucleic acid repair. Plays an important role in protecting cells from carbonyl stress. KEYWORDS: Cytoplasm;DNA damage;DNA repair;Hydrolase;Stress response SUBCELLULAR LOCATION: Cytoplasm " A5JYS0,"PROTEIN NAMES: Glutathione gamma-glutamylcysteinyltransferase (Phytochelatin synthase) (PC synthase) PROTEIN FAMILY: Phytochelatin synthase family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the phytochelatin synthase family. FUNCTION: Involved in the synthesis of phytochelatins, which are heavy metal binding proteins required for the detoxification of heavy metals such as cadmium, arsenic and copper. KEYWORDS: Acyltransferase;Alternative splicing;Cadmium;Metal-binding;Reference proteome;Transferase " A5K9M4,"PROTEIN NAMES: Purine nucleoside phosphorylase (PvPNP) PROTEIN FAMILY: PNP/MTAP phosphorylase family ORGANISM: Plasmodium vivax (strain Salvador I) SIMILARITY: Belongs to the PNP/MTAP phosphorylase family. FUNCTION: As part of the purine salvage pathway, catalyzes the phosphorolytic breakdown of the N-glycosidic bond in the beta-(deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate. Preferentially acts on inosine and guanosine, and to a lesser extent on 2'-deoxyinosine and 2'-deoxyguanosine. PATHWAY: Purine metabolism; purine nucleoside salvage. KEYWORDS: 3D-structure;Glycosyltransferase;Purine salvage;Reference proteome;Transferase " A5PF10,"PROTEIN NAMES: Sialidase-1 (Acetylneuraminyl hydrolase) (Lysosomal sialidase) (N-acetyl-alpha-neuraminidase 1) PROTEIN FAMILY: Glycosyl hydrolase 33 family ORGANISM: Sus scrofa (Pig) SIMILARITY: Belongs to the glycosyl hydrolase 33 family. FUNCTION: Catalyzes the removal of sialic acid (N-acetylneuraminic acid) moieties from glycoproteins and glycolipids. To be active, it is strictly dependent on its presence in the multienzyme complex. Appears to have a preference for alpha 2-3 and alpha 2-6 sialyl linkage (By similarity). KEYWORDS: Carbohydrate metabolism;Cell membrane;Cytoplasmic vesicle;Glycoprotein;Glycosidase;Hydrolase;Lipid degradation;Lipid metabolism;Lysosome;Membrane;Phosphoprotein;Reference proteome;Repeat;Signal SUBCELLULAR LOCATION: Lysosome membrane ; Peripheral membrane protein ; Lumenal side Lysosome lumen Cell membrane Cytoplasmic vesicle Note=Localized not only on the inner side of the lysosomal membrane and in the lysosomal lumen, but also on the plasma membrane and in intracellular vesicles. " A5PJP6,"PROTEIN NAMES: Lys-63-specific deubiquitinase BRCC36 (BRCA1-A complex subunit BRCC36) (BRCA1/BRCA2-containing complex subunit 3) (BRCA1/BRCA2-containing complex subunit 36) (BRISC complex subunit BRCC36) PROTEIN FAMILY: Peptidase M67A family, BRCC36 subfamily ORGANISM: Bos taurus (Bovine) SIMILARITY: Belongs to the peptidase M67A family. BRCC36 subfamily. FUNCTION: Metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. Does not have activity toward 'Lys-48'-linked polyubiquitin chains. Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). In the BRCA1-A complex, it specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX, antagonizing the RNF8-dependent ubiquitination at double-strand breaks (DSBs). Catalytic subunit of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin in various substrates. Mediates the specific 'Lys-63'-specific deubiquitination associated with the COP9 signalosome complex (CSN), via the interaction of the BRISC complex with the CSN complex. The BRISC complex is required for normal mitotic spindle assembly and microtubule attachment to kinetochores via its role in deubiquitinating NUMA1. Plays a role in interferon signaling via its role in the deubiquitination of the interferon receptor IFNAR1; deubiquitination increases IFNAR1 activity by enhancing its stability and cell surface expression (By similarity). Acts as a regulator of the NLRP3 inflammasome by mediating deubiquitination of NLRP3, leading to NLRP3 inflammasome assembly (By similarity). Down-regulates the response to bacterial lipopolysaccharide (LPS) via its role in IFNAR1 deubiquitination (By similarity). Deubiquitinates HDAC1 and PWWP2B leading to their stabilization (By similarity). KEYWORDS: Acetylation;Alternative splicing;Cell cycle;Cell division;Chromatin regulator;Cytoplasm;Cytoskeleton;DNA damage;DNA repair;Hydrolase;Metal-binding;Metalloprotease;Mitosis;Nucleus;Phosphoprotein;Protease;Reference proteome;Ubl conjugation pathway;Zinc SUBCELLULAR LOCATION: Nucleus Cytoplasm Cytoplasm, cytoskeleton, spindle pole Note=Localizes at sites of DNA damage at double-strand breaks (DSBs). Interaction with ABRAXAS2 retains BRCC3 in the cytoplasm. " A5U493,"PROTEIN NAMES: Beta-lactamase (Ambler class A beta-lactamase) PROTEIN FAMILY: Class-A beta-lactamase family ORGANISM: Mycobacterium tuberculosis (strain ATCC 25177 / H37Ra) SIMILARITY: Belongs to the class-A beta-lactamase family. FUNCTION: Extended spectrum beta-lactamase (ESBL) that inactivates beta-lactam antibiotics by hydrolyzing the amide group of the beta-lactam ring. Exhibits predominant penicillinase activity. Also displays high levels of cephalosporinase activity as well as measurable activity with carbapenems, including imipenem and meropenem. Plays a primary role in the intrinsic resistance of M.tuberculosis to beta-lactam antibiotics. KEYWORDS: Antibiotic resistance;Hydrolase;Periplasm;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Periplasm Secreted MISCELLANEOUS: The class A beta-lactamase family has a specific amino-acid numbering system, sometimes called Ambler or ABL numbering and often misspelt as Amber. A multiple sequence alignment was used to derive a consensus sequence and then the consensus was numbered taking into account insertions and deletions. This allows use of identical numbers, e.g. for active site residues, despite differences in protein length. UniProt always uses natural numbering of residues, hence there appear to be differences in numbering between this entry and some papers." A5U4N0,"PROTEIN NAMES: Adenosine kinase (ADK) (AK) PROTEIN FAMILY: Carbohydrate kinase PfkB family ORGANISM: Mycobacterium tuberculosis (strain ATCC 25177 / H37Ra) SIMILARITY: Belongs to the carbohydrate kinase PfkB family. FUNCTION: Catalyzes the phosphorylation of adenosine to adenosine monophosphate (AMP). Can also catalyze the phosphorylation of the adenosine analog 2-methyladenosine (methyl-Ado) to methyl-AMP, the first step in the metabolism of this compound to an active form that displays antitubercular activity. Is not active on guanosine, inosine, deoxyadenosine, cytidine, uridine, or thymidine. Prefers dGTP and GTP to ATP as phosphate donors in vitro. PATHWAY: Purine metabolism; AMP biosynthesis via salvage pathway; AMP from adenosine: step 1/1. KEYWORDS: ATP-binding;Direct protein sequencing;Kinase;Magnesium;Nucleotide-binding;Purine salvage;Reference proteome;Transferase " A5X5Y0,"PROTEIN NAMES: 5-hydroxytryptamine receptor 3E (5-HT3-E) (5-HT3E) (Serotonin receptor 3E) PROTEIN FAMILY: Ligand-gated ion channel family, 5-hydroxytryptamine receptor subfamily, HTR3E sub-subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the ligand-gated ion channel (TC 1.A.9) family. 5-hydroxytryptamine receptor (TC 1.A.9.2) subfamily. HTR3E sub-subfamily. FUNCTION: Forms serotonin (5-hydroxytryptamine/5-HT3)-activated cation-selective channel complexes, which when activated cause fast, depolarizing responses in neurons. KEYWORDS: Alternative splicing;Cell membrane;Disulfide bond;Glycoprotein;Ion channel;Ion transport;Ligand-gated ion channel;Membrane;Postsynaptic cell membrane;Receptor;Reference proteome;Signal;Synapse;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Postsynaptic cell membrane ; Multi-pass membrane protein Cell membrane ; Multi-pass membrane protein Note=Presumably retained within the endoplasmic reticulum unless complexed with HTR3A. " A6H687,"PROTEIN NAMES: SAC3 domain-containing protein 1 (SAC3 homology domain-containing protein 1) PROTEIN FAMILY: SAC3 family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the SAC3 family. FUNCTION: Involved in centrosome duplication and mitotic progression. KEYWORDS: Cell cycle;Cell division;Cytoplasm;Cytoskeleton;Mitosis;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Cytoplasm, cytoskeleton, spindle Note=Localizes on centrosomes in interphase cells and at spindles in mitosis. " A6NGQ2,"PROTEIN NAMES: Oocyte-expressed protein homolog (KH homology domain-containing protein 2) (Oocyte- and embryo-specific protein 19) (hOEP19) PROTEIN FAMILY: KHDC1 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the KHDC1 family. FUNCTION: As part of the OOEP-KHDC3L scaffold, recruits BLM and TRIM25 to DNA replication forks, thereby promoting the ubiquitination of BLM by TRIM25, enhancing BLM retainment at replication forks and therefore promoting stalled replication fork restart (By similarity). Positively regulates the homologous recombination-mediated DNA double-strand break (DSB) repair pathway by regulating ATM activation and RAD51 recruitment to DSBs in oocytes (By similarity). Thereby contributes to oocyte survival and the resumption and completion of meiosis (By similarity). As a member of the subcortical maternal complex (SCMC), plays an essential role for zygotes to progress beyond the first embryonic cell divisions via regulation of actin dynamics (By similarity). Required for the formation of F-actin cytoplasmic lattices in oocytes which in turn are responsible for symmetric division of zygotes via the regulation of mitotic spindle formation and positioning (By similarity). KEYWORDS: Cytoplasm;Nucleus;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Nucleus " A6NJ46,"PROTEIN NAMES: Homeobox protein Nkx-6.3 ORGANISM: Homo sapiens (Human) FUNCTION: Putative transcription factor, which may be involved in patterning of central nervous system and pancreas. KEYWORDS: Alternative splicing;DNA-binding;Homeobox;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " A6NNM8,"PROTEIN NAMES: Tubulin polyglutamylase TTLL13 (Tubulin tyrosine ligase like 13) (Tubulin tyrosine ligase-like family member 13 pseudogene) (Tubulin--tyrosine ligase-like protein 13) PROTEIN FAMILY: Tubulin--tyrosine ligase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the tubulin--tyrosine ligase family. FUNCTION: Polyglutamylase which modifies tubulin, generating polyglutamate side chains of variable lengths on the gamma-carboxyl group of specific glutamate residues within the C-terminal tail of tubulin. Mediates ATP-dependent polyglutamate side-chain elongation of the polyglutamylation reaction but not the initiation step. Preferentially modifies the alpha-tubulin tail over a beta-tail. KEYWORDS: Alternative splicing;ATP-binding;Coiled coil;Ligase;Magnesium;Metal-binding;Microtubule;Nucleotide-binding;Reference proteome MISCELLANEOUS: [Isoform 2]: May be due to an intron retention." O69199,"PROTEIN NAMES: Adenylate cyclase CyaB (ATP pyrophosphate-lyase) (Adenylyl cyclase) PROTEIN FAMILY: Adenylyl cyclase CyaB family ORGANISM: Aeromonas hydrophila SIMILARITY: Belongs to the adenylyl cyclase CyaB family. FUNCTION: In vitro, CyaB catalyzes the biosynthesis of cyclic AMP (cAMP) from ATP. It seems that under the physiological conditions CyaB has no function in cAMP processes. In vitro, it is also able to hydrolyze substrates such as thiamine triphosphate (ThTP) and inorganic triphosphate (PPPi) at a low rate. It has a slight preference for ThTP over ATP and PPPi in the presence of manganese ions. This PPPase activity is probably not of physiological importance. KEYWORDS: ATP-binding;cAMP biosynthesis;Cytoplasm;Direct protein sequencing;Lyase;Nucleotide-binding SUBCELLULAR LOCATION: Cytoplasm " O69729,PROTEIN NAMES: Probable sensor histidine kinase TcrY ORGANISM: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) FUNCTION: Member of the two-component regulatory system TcrY/TcrX. Activates TcrX by phosphorylation. KEYWORDS: ATP-binding;Cell membrane;Kinase;Membrane;Nucleotide-binding;Phosphoprotein;Reference proteome;Transferase;Transmembrane;Transmembrane helix;Two-component regulatory system SUBCELLULAR LOCATION: Cell membrane ; Single-pass membrane protein O70131,"PROTEIN NAMES: Ninjurin-1 (Nerve injury-induced protein 1) [Cleaved into: Secreted ninjurin-1 (Soluble ninjurin-1) (sNinJ1)] PROTEIN FAMILY: Ninjurin family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the ninjurin family. FUNCTION: [Ninjurin-1]: Effector of necroptotic and pyroptotic programmed cell death that mediates plasma membrane rupture (cytolysis). Acts downstream of Gasdermin (GSDMA, GSDMB, GSDMC, GSDMD, or GSDME) or MLKL during pyroptosis or necroptosis, respectively: oligomerizes in response to death stimuli and promotes plasma membrane rupture by introducing hydrophilic faces of 2 alpha helices into the hydrophobic membrane, leading to release intracellular molecules named damage-associated molecular patterns (DAMPs) that propagate the inflammatory response. Acts as a regulator of Toll-like receptor 4 (TLR4) signaling triggered by lipopolysaccharide (LPS) during systemic inflammation; directly binds LPS. Involved in leukocyte migration during inflammation by promoting transendothelial migration of macrophages via homotypic binding. Promotes the migration of monocytes across the brain endothelium to central nervous system inflammatory lesions (By similarity). Also acts as a homophilic transmembrane adhesion molecule involved in various processes such as axonal growth, cell chemotaxis and angiogenesis. Promotes cell adhesion by mediating homophilic interactions via its extracellular N-terminal adhesion motif (N-NAM). Involved in the progression of the inflammatory stress by promoting cell-to-cell interactions between immune cells and endothelial cells. Plays a role in nerve regeneration by promoting maturation of Schwann cells. Acts as a regulator of angiogenesis. Promotes the formation of new vessels by mediating the interaction between capillary pericyte cells and endothelial cells. Also mediates vascular functions in penile tissue as well as vascular formation. Promotes osteoclasts development by enhancing the survival of prefusion osteoclasts. Also involved in striated muscle growth and differentiation. Also involved in cell senescence in a p53/TP53 manner, possibly by acting as an indirect regulator of p53/TP53 mRNA translation.; FUNCTION: [Secreted ninjurin-1]: Secreted form generated by cleavage, which has chemotactic activity. Acts as an anti-inflammatory mediator by promoting monocyte recruitment, thereby ameliorating atherosclerosis. KEYWORDS: Acetylation;Angiogenesis;Behavior;Cell adhesion;Cell membrane;Cytolysis;Glycoprotein;Inflammatory response;Membrane;Phosphoprotein;Reference proteome;Secreted;Synapse;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: [Ninjurin-1]: Cell membrane ; Multi-pass membrane protein Synaptic cell membrane ; Multi-pass membrane protein.; SUBCELLULAR LOCATION: [Secreted ninjurin-1]: Secreted " O70143,PROTEIN NAMES: SHC-transforming protein 3 (Neuronal Shc) (N-Shc) (SHC-transforming protein C) (Src homology 2 domain-containing-transforming protein C3) (SH2 domain protein C3) ORGANISM: Rattus norvegicus (Rat) FUNCTION: Signaling adapter that couples activated growth factor receptors to signaling pathway in neurons. Involved in the signal transduction pathways of neurotrophin-activated Trk receptors in cortical neurons (By similarity). KEYWORDS: Alternative initiation;Phosphoprotein;Reference proteome;SH2 domain O70212,"PROTEIN NAMES: 5-hydroxytryptamine receptor 3A (5-HT3-A) (5-HT3A) (5-hydroxytryptamine receptor 3) (5-HT-3) (5-HT3R) (Serotonin receptor 3A) (Serotonin-gated ion channel receptor) PROTEIN FAMILY: Ligand-gated ion channel family, 5-hydroxytryptamine receptor subfamily, HTR3A sub-subfamily ORGANISM: Cavia porcellus (Guinea pig) SIMILARITY: Belongs to the ligand-gated ion channel (TC 1.A.9) family. 5-hydroxytryptamine receptor (TC 1.A.9.2) subfamily. HTR3A sub-subfamily. FUNCTION: Forms serotonin (5-hydroxytryptamine/5-HT3)-activated cation-selective channel complexes, which when activated cause fast, depolarizing responses in neurons. KEYWORDS: Alternative splicing;Cell membrane;Disulfide bond;Glycoprotein;Ion channel;Ion transport;Ligand-gated ion channel;Membrane;Postsynaptic cell membrane;Receptor;Reference proteome;Signal;Synapse;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Postsynaptic cell membrane ; Multi-pass membrane protein Cell membrane ; Multi-pass membrane protein " O70230,PROTEIN NAMES: Zinc finger protein 143 (Zfp-143) (Selenocysteine tRNA gene transcription-activating factor) (mStaf) PROTEIN FAMILY: GLI C2H2-type zinc-finger protein family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the GLI C2H2-type zinc-finger protein family. FUNCTION: Transcriptional activator. Activates the gene for selenocysteine tRNA (tRNAsec). Binds to the SPH motif of small nuclear RNA (snRNA) gene promoters. Participates in efficient U6 RNA polymerase III transcription via its interaction with CHD8 (By similarity). KEYWORDS: Acetylation;Activator;Alternative splicing;DNA-binding;Isopeptide bond;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Repeat;Transcription;Transcription regulation;Ubl conjugation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus O70263,"PROTEIN NAMES: E3 ubiquitin-protein ligase LNX (Ligand of Numb protein X 1) (Ligand of Numb-binding protein 1) (Numb-binding protein 1) (RING-type E3 ubiquitin transferase LNX) ORGANISM: Mus musculus (Mouse) FUNCTION: E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of NUMB. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates ubiquitination of isoform p66 and isoform p72 of NUMB, but not that of isoform p71 or isoform p65.; FUNCTION: Isoform 2 provides an endocytic scaffold for IGSF5/JAM4. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: 3D-structure;Alternative splicing;Cytoplasm;Metal-binding;Phosphoprotein;Reference proteome;Repeat;Transferase;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cytoplasm. " O70423,PROTEIN NAMES: Amine oxidase [copper-containing] 3 (Amine oxidase copper-containing 3) (Copper amine oxidase) (Semicarbazide-sensitive amine oxidase) (SSAO) (Vascular adhesion protein 1) (VAP-1) PROTEIN FAMILY: Copper/topaquinone oxidase family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the copper/topaquinone oxidase family. FUNCTION: Catalyzes the oxidative deamination of primary amines to the corresponding aldehydes with the concomitant production of hydrogen peroxide and ammonia. Has a preference for the primary monoamines methylamine and benzylamine. Could also act on 2-phenylethylamine but much less efficiently. At endothelial cells surface can also function as a cell adhesion protein that participates in lymphocyte extravasation and recirculation by mediating the binding of lymphocytes to peripheral lymph node vascular endothelial cells in an L-selectin-independent fashion. KEYWORDS: Alternative splicing;Calcium;Cell adhesion;Cell membrane;Copper;Disulfide bond;Glycoprotein;Membrane;Metal-binding;Oxidoreductase;Reference proteome;Signal-anchor;TPQ;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type II membrane protein O70491,"PROTEIN NAMES: Receptor for retinol uptake STRA6 (Retinoic acid-responsive protein) (Retinol-binding protein receptor STRA6) (Stimulated by retinoic acid gene 6 protein) ORGANISM: Mus musculus (Mouse) FUNCTION: Functions as a retinol transporter. Accepts all-trans retinol from the extracellular retinol-binding protein RBP4, facilitates retinol transport across the cell membrane, and then transfers retinol to the cytoplasmic retinol-binding protein RBP1. Retinol uptake is enhanced by LRAT, an enzyme that converts retinol to all-trans retinyl esters, the storage forms of vitamin A (By similarity). Contributes to the activation of a signaling cascade that depends on retinol transport and LRAT-dependent generation of retinol metabolites that then trigger activation of JAK2 and its target STAT5, and ultimately increase the expression of SOCS3 and inhibit cellular responses to insulin. Important for the homeostasis of vitamin A and its derivatives, such as retinoic acid and 11-cis-retinal. STRA6-mediated transport is particularly important in the eye, and under conditions of dietary vitamin A deficiency. Does not transport retinoic acid (By similarity). KEYWORDS: Cell membrane;Glycoprotein;Membrane;Phosphoprotein;Receptor;Reference proteome;Retinol-binding;Transmembrane;Transmembrane helix;Transport;Vitamin A SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Note=In the retinal pigment epithelium localizes to the basolateral membrane. Plasma membrane of the basal pole of Sertoli cells, absent in plasma membrane of neighboring spermatogonia. MISCELLANEOUS: The retinoic acid-induced activation is impaired in retinoic acid receptor gamma-null F9 cells." O70514,"PROTEIN NAMES: Fibroblast growth factor-binding protein 1 (FGF-BP) (FGF-BP1) (FGF-binding protein 1) (FGFBP-1) PROTEIN FAMILY: Fibroblast growth factor-binding protein family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the fibroblast growth factor-binding protein family. FUNCTION: Acts as a carrier protein that releases fibroblast-binding factors (FGFs) from the extracellular matrix (EM) storage and thus enhances the mitogenic activity of FGFs. Enhances FGF2 signaling during tissue repair, angiogenesis and in tumor growth (By similarity). KEYWORDS: Cell membrane;Disulfide bond;Glycoprotein;Growth factor binding;Membrane;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted, extracellular space Cell membrane ; Peripheral membrane protein Note=Extracellular and plasma membrane-associated. MISCELLANEOUS: Expression is significantly up-regulated in skin papillomas and carcinomas." O70531,"PROTEIN NAMES: Sulfate transporter (Diastrophic dysplasia protein homolog) (Solute carrier family 26 member 2) PROTEIN FAMILY: SLC26A/SulP transporter family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the SLC26A/SulP transporter (TC 2.A.53) family. FUNCTION: Sulfate transporter which mediates sulfate uptake into chondrocytes in order to maintain adequate sulfation of proteoglycans which is needed for cartilage development. Mediates electroneutral anion exchange of sulfate ions for oxalate ions, sulfate and oxalate ions for chloride and/or hydroxyl ions and chloride ions for bromide, iodide and nitrate ions (By similarity). The coupling of sulfate transport to both hydroxyl and chloride ions likely serves to ensure transport at both acidic pH when most sulfate uptake is mediated by sulfate-hydroxide exchange and alkaline pH when most sulfate uptake is mediated by sulfate-chloride exchange (By similarity). Essential for chondrocyte proliferation, differentiation and cell size expansion (By similarity). KEYWORDS: Cell membrane;Glycoprotein;Membrane;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Apical cell membrane ; Multi-pass membrane protein " O73673,"PROTEIN NAMES: Glucocorticoid receptor (GR) (Nuclear receptor subfamily 3 group C member 1) PROTEIN FAMILY: Nuclear hormone receptor family, NR3 subfamily ORGANISM: Paralichthys olivaceus (Bastard halibut) (Hippoglossus olivaceus) SIMILARITY: Belongs to the nuclear hormone receptor family. NR3 subfamily. FUNCTION: Receptor for glucocorticoids (GC). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE), both for nuclear and mitochondrial DNA, and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Involved in chromatin remodeling. Plays a role in rapid mRNA degradation by binding to the 5' UTR of target mRNAs and interacting with PNRC2 in a ligand-dependent manner which recruits the RNA helicase UPF1 and the mRNA-decapping enzyme DCP1A, leading to RNA decay. Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth. Mediates glucocorticoid-induced apoptosis. Promotes accurate chromosome segregation during mitosis. May act as a tumor suppressor. May play a negative role in adipogenesis through the regulation of lipolytic and antilipogenic gene expression. KEYWORDS: Chromatin regulator;Cytoplasm;Cytoskeleton;DNA-binding;Lipid-binding;Metal-binding;Mitochondrion;Nucleus;Receptor;Steroid-binding;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cytoplasm Nucleus Mitochondrion Cytoplasm, cytoskeleton, spindle Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Note=After ligand activation, translocates from the cytoplasm to the nucleus. " O73864,"PROTEIN NAMES: Protein Wnt-11 PROTEIN FAMILY: Wnt family ORGANISM: Danio rerio (Zebrafish) (Brachydanio rerio) SIMILARITY: Belongs to the Wnt family. FUNCTION: Ligand for members of the frizzled family of seven transmembrane receptors. May play a role in the formation of dermal structure in limb buds. Is likely to signal over only few cell diameters (By similarity). KEYWORDS: Developmental protein;Disulfide bond;Extracellular matrix;Glycoprotein;Lipoprotein;Reference proteome;Secreted;Signal;Wnt signaling pathway SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix. " O74113,PROTEIN NAMES: Cell division control protein 45 homolog (Suppressor of nda4 protein) PROTEIN FAMILY: CDC45 family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the CDC45 family. FUNCTION: Required for initiation of chromosomal DNA replication. May have a role in regulating the MCM proteins nda1 and nda4. KEYWORDS: Cell cycle;DNA replication;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus O74189,"PROTEIN NAMES: Dolichyl-phosphate-mannose--protein mannosyltransferase 1 (Protein mannosyltransferase 1) PROTEIN FAMILY: Glycosyltransferase 39 family ORGANISM: Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) SIMILARITY: Belongs to the glycosyltransferase 39 family. FUNCTION: Protein mannosyltransferase (PMT) involved in hyphal growth and drug sensitivity. Transfers mannose from Dol-P-mannose to Ser or Thr residues on proteins. PMT1, PMT2 and PMT4 account for most of the protein-O-glycosylation activity, while PMT5 and PMT6 may specifically modulate a much narrower spectrum of target proteins. Accounts for the O-glycosylation of the cell wall proteins KRE9, PIR2, RHD3, and ALS1, as well as the SEC20 t-SNARE component. O-glycosylation of SEC20 is essential for its stability. Required for filamentation and early phases of biofilm formation. PATHWAY: Protein modification; protein glycosylation. KEYWORDS: Endoplasmic reticulum;Glycoprotein;Glycosyltransferase;Membrane;Reference proteome;Repeat;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass membrane protein " O74288,"PROTEIN NAMES: Alpha-L-arabinofuranosidase B (ABF B) (Arabinosidase B) PROTEIN FAMILY: Glycosyl hydrolase 54 family ORGANISM: Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) SIMILARITY: Belongs to the glycosyl hydrolase 54 family. FUNCTION: Alpha-L-arabinofuranosidase involved in the degradation of arabinoxylan, a major component of plant hemicellulose. Able to hydrolyze 1,5-, 1,3- and 1,2-alpha-linkages not only in L-arabinofuranosyl oligosaccharides, but also in polysaccharides containing terminal non-reducing L-arabinofuranoses in side chains, like L-arabinan, arabinogalactan and arabinoxylan. PATHWAY: Glycan metabolism; L-arabinan degradation. KEYWORDS: Carbohydrate metabolism;Disulfide bond;Glycoprotein;Glycosidase;Hydrolase;Polysaccharide degradation;Reference proteome;Secreted;Signal;Xylan degradation SUBCELLULAR LOCATION: Secreted " O74309,PROTEIN NAMES: Histone transcription regulator slm9 PROTEIN FAMILY: WD repeat HIR1 family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the WD repeat HIR1 family. FUNCTION: Probably required for replication-independent chromatin assembly (By similarity). Required for transcriptional silencing in the outer repeat (otr) centromeric repeats and the Tf2 long terminal repeat retrotransposons. May play an indirect role in the regulation of cdc2 and/or wee1 at the G2/M stage of mitosis. KEYWORDS: Chromatin regulator;Cytoplasm;Nucleus;Phosphoprotein;Reference proteome;Repeat;Repressor;Transcription;Transcription regulation;WD repeat SUBCELLULAR LOCATION: Cytoplasm. Nucleus. O74327,"PROTEIN NAMES: Vacuolar amino acid transporter 5 PROTEIN FAMILY: Amino acid/polyamine transporter 2 family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the amino acid/polyamine transporter 2 family. FUNCTION: Vacuolar amino acid transporter involved in the vacuolar uptake of histidine, glutamate, tyrosine, arginine, lysine, and serine. Required for sporulation. KEYWORDS: Amino-acid transport;Membrane;Reference proteome;Sporulation;Transmembrane;Transmembrane helix;Transport;Vacuole SUBCELLULAR LOCATION: Vacuole membrane ; Multi-pass membrane protein " O74352,PROTEIN NAMES: Protein hob1 (Homolog of Bin1) ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) FUNCTION: Has a role in DNA damage signaling as a part of stress response processes. KEYWORDS: Coiled coil;Phosphoprotein;Reference proteome;SH3 domain O74465,PROTEIN NAMES: Helicase required for RNAi-mediated heterochromatin assembly 1 ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) FUNCTION: Has a role in the RNA interference (RNAi) pathway which is important for heterochromatin formation and accurate chromosome segregation. A member of the RNA-directed RNA polymerase complex (RDRC) which is involved in the generation of small interfering RNAs (siRNAs) and mediate their association with the RNA-induced transcriptional silencing (RITS) complex. RITS acts as a priming complex for dsRNA synthesis at the site of non-coding centromeric RNA. KEYWORDS: ATP-binding;Cell cycle;Chromosome partition;Cytoplasm;Helicase;Hydrolase;Nucleotide-binding;Nucleus;Phosphoprotein;Reference proteome;RNA-binding;RNA-mediated gene silencing SUBCELLULAR LOCATION: Cytoplasm. Nucleus. O74518,PROTEIN NAMES: Poly(A) RNA polymerase cid12 (PAP) (Caffeine-induced death protein 12) (Polynucleotide adenylyltransferase cid12) PROTEIN FAMILY: DNA polymerase type-B-like family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the DNA polymerase type-B-like family. FUNCTION: Has a role in the RNA interference (RNAi) pathway which is important for heterochromatin formation and accurate chromosome segregation. A member of the RNA-directed RNA polymerase complex (RDRC) which is involved in the generation of small interfering RNAs (siRNAs) and mediate their association with the RNA-induced transcriptional silencing (RITS) complex. RITS acts as a priming complex for dsRNA synthesis at the site of non-coding centromeric RNA. KEYWORDS: ATP-binding;Cell cycle;Chromosome partition;Cytoplasm;Magnesium;Manganese;Metal-binding;Nucleotide-binding;Nucleus;Phosphoprotein;Reference proteome;RNA-binding;RNA-mediated gene silencing;Transferase SUBCELLULAR LOCATION: Cytoplasm Nucleus O74536,"PROTEIN NAMES: SNF1-like protein kinase ssp2 PROTEIN FAMILY: Protein kinase superfamily, CAMK Ser/Thr protein kinase family, SNF1 subfamily ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily. FUNCTION: Serine/threonine protein kinase essential for release from glucose repression via the phosphorylation of scr1 upon glucose deprivation. Catalytic subunit of the AMP-activated protein kinase complex also known as the SNF1 kinase complex (Snf1c), a central regulator of cellular energy homeostasis, which, in response to a fall in intracellular ATP levels, activates energy-producing pathways and inhibits energy-consuming processes. The complex phosphorylates histone H3 to form H3S10ph, which promotes H3K14ac formation, leading to transcriptional activation through TBP recruitment to the promoters (By similarity). Regulates proper cell cycle exit and sexual differentiation. Regulates also ste11 levels under nitrogen deprivation. KEYWORDS: 3D-structure;ATP-binding;Carbohydrate metabolism;Cytoplasm;Kinase;Nucleotide-binding;Nucleus;Phosphoprotein;Reference proteome;Serine/threonine-protein kinase;Transferase SUBCELLULAR LOCATION: Cytoplasm Nucleus Note=Nuclear translocation occurs under nitrogen and glucose starvation conditions and depends on Thr-189 phosphorylation by ssp1. " O74563,PROTEIN NAMES: E3 ubiquitin-protein ligase brl2 (BRE1-like protein 2) (RING finger protein 1) (RING-type E3 ubiquitin transferase brl2) PROTEIN FAMILY: BRE1 family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the BRE1 family. FUNCTION: E3 ubiquitin-protein ligase which belongs to the histone H2B ubiquitin ligase complex (HULC) which mediates monoubiquitination of histone H2B to form H2BK123ub1. H2BK123ub1 gives a specific tag for epigenetic transcriptional activation and is also a prerequisite for H3K4me and H3K79me formation. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Chromatin regulator;Coiled coil;Metal-binding;Nucleus;Reference proteome;Transferase;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus O74627,"PROTEIN NAMES: Cyclin pch1 (Pombe cyclin C homolog 1) PROTEIN FAMILY: Cyclin family, Cyclin C subfamily ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the cyclin family. Cyclin C subfamily. FUNCTION: Essential for progression through the whole cell cycle. KEYWORDS: Cell cycle;Cell division;Cyclin;Nucleus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Nucleus " O74653,PROTEIN NAMES: Protein pob1 (BOI protein homolog) ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) FUNCTION: Has a role in cell elongation and separation. KEYWORDS: Cytoplasm;Membrane;Phosphoprotein;Reference proteome;SH3 domain SUBCELLULAR LOCATION: Cytoplasm Membrane ; Peripheral membrane protein Note=Membrane-associated at the cell tips during interphase. O74739,"PROTEIN NAMES: Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase (PNGase) (Peptide:N-glycanase 1) PROTEIN FAMILY: Transglutaminase-like superfamily, PNGase family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the transglutaminase-like superfamily. PNGase family. FUNCTION: Specifically deglycosylates the denatured form of N-linked glycoproteins in the cytoplasm and assists their proteasome-mediated degradation. Cleaves the beta-aspartyl-glucosamine (GlcNAc) of the glycan and the amide side chain of Asn, converting Asn to Asp. Prefers proteins containing high-mannose over those bearing complex type oligosaccharides. Can recognize misfolded proteins in the endoplasmic reticulum that are exported to the cytosol to be destroyed and deglycosylate them, while it has no activity toward native proteins. Deglycosylation is a prerequisite for subsequent proteasome-mediated degradation of some, but not all, misfolded glycoproteins (By similarity). KEYWORDS: Cytoplasm;Hydrolase;Metal-binding;Nucleus;Reference proteome;Zinc SUBCELLULAR LOCATION: Cytoplasm Nucleus " O74757,"PROTEIN NAMES: ERAD-associated E3 ubiquitin-protein ligase hrd1 (RING-type E3 ubiquitin transferase hrd1) PROTEIN FAMILY: HRD1 family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the HRD1 family. FUNCTION: E3 ubiquitin-protein ligase which accepts ubiquitin specifically from endoplasmic reticulum-associated E2 ligases, and transfers it to substrates promoting their degradation. Mediates the degradation of endoplasmic reticulum proteins (ERQC), also called ER-associated degradation (ERAD). Component of the hrd1 ubiquitin ligase complex, which is part of the ERAD-L and ERAD-M pathways responsible for the rapid degradation of soluble lumenal and membrane proteins with misfolded lumenal domains (ERAD-L), or ER-membrane proteins with misfolded transmembrane domains (ERAD-M) (By similarity). Together with ubc7, required for the degradation of the transcription factor sre1 precursor in the absence of its binding partner scp1. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Endoplasmic reticulum;Membrane;Metal-binding;Phosphoprotein;Reference proteome;Transferase;Transmembrane;Transmembrane helix;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein " O74804,"PROTEIN NAMES: Telomere elongation protein est1 (Ever shorter telomeres protein 1) PROTEIN FAMILY: EST1 family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the EST1 family. FUNCTION: Directly involved in telomere replication. Associates with telomerase and during its interaction with trt1, telomerase activity is promoted. KEYWORDS: Chromosome;DNA-binding;Nucleus;Reference proteome;Telomere SUBCELLULAR LOCATION: Nucleus Chromosome, telomere " O74850,"PROTEIN NAMES: Diacylglycerol O-acyltransferase 1 (Diglyceride acyltransferase) (Triacylglycerol synthase) (TAG synthase) PROTEIN FAMILY: Diacylglycerol acyltransferase family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the diacylglycerol acyltransferase family. FUNCTION: Catalyzes the terminal and only committed step in triacylglycerol (TAG) synthesis by using diacylglycerol (DAG) and fatty acyl-CoA as substrates. Required for storage lipid synthesis. Major DAG esterifying enzyme in stationary phase when TAG production is particularly active. Involved in lipid particle synthesis from the endoplasmic reticulum, promoting localized TAG production at discrete ER subdomains. PATHWAY: Glycerolipid metabolism; triacylglycerol biosynthesis. KEYWORDS: Acyltransferase;Endoplasmic reticulum;Glycerol metabolism;Lipid biosynthesis;Lipid droplet;Lipid metabolism;Membrane;Reference proteome;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Lipid droplet Endoplasmic reticulum membrane ; Multi-pass membrane protein Note=Localizes to sites of lipid droplet biogenesis in the endoplasmic reticulum. " O74874,"PROTEIN NAMES: CCR4-Not complex 3'-5'-exoribonuclease subunit Ccr4 (Carbon catabolite repressor protein 4) (Cytoplasmic deadenylase) (Glucose-repressible alcohol dehydrogenase transcriptional effector) PROTEIN FAMILY: CCR4/nocturin family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the CCR4/nocturin family. FUNCTION: Acts as a catalytic component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover (By similarity). Ccr4 has 3'-5' RNase activity with a strong preference for polyadenylated substrates and also low exonuclease activity towards single-stranded DNA (By similarity). Participates in the shortening of poly(A)-tails. Erh1-mmi1 complex-mediated recruitment of CCR4-NOT to target RNAs promotes heterochromatin formation at RNAi-dependent heterochromatin domains (HOODs), including a subset of meiotic genes, lncRNAs and retrotransposons. Recruitment of the CCR4-NOT complex to rDNA promotes rDNA heterochromatin assembly. KEYWORDS: Activator;Cytoplasm;Exonuclease;Hydrolase;Leucine-rich repeat;Magnesium;Metal-binding;Nuclease;Nucleus;Reference proteome;Repeat;Repressor;RNA-binding;Transcription;Transcription regulation SUBCELLULAR LOCATION: Cytoplasm Nucleus " O74958,"PROTEIN NAMES: RNA binding exosome specificity factor Mmi1 (Meiotic mRNA interception protein 1) (YTH domain-containing protein mmi1) ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) FUNCTION: RNA-binding protein that recognizes and binds N6-methyladenosine (m6A)-containing RNAs, a modification present at internal sites of mRNAs and some non-coding RNAs (By similarity). Functions alone and as part of the erh1-mmi1 complex, to recruit the CCR4-NOT complex and the NURS complex to target RNAs. Suppresses the meiotic program during vegetative growth and promotes the meiotic program during mating. Binds to DSR (determinant of selective removal) regions in meiotic mRNA, and recruits the NURS complex to targets. Recruitment of NURS complex to target mRNAs promotes mRNA decay by engagement of the nuclear exosome, and formation of heterochromatin islands at meiotic genes silenced by the exosome. Recruitment of the CCR4-NOT complex to target RNAs promotes heterochromatin formation at RNAi-dependent heterochromatin domains (HOODs), including a subset of meiotic genes, lncRNAs and retrotransposons. Recruitment of the CCR4-NOT complex to rDNA promotes rDNA heterochromatin assembly. Promotes non-canonical transcription termination at meiotic genes and prevents lncRNA transcription from invading and repressing adjacent genes. KEYWORDS: 3D-structure;Nucleus;Phosphoprotein;Reference proteome;RNA-binding SUBCELLULAR LOCATION: Nucleus " O74971,"PROTEIN NAMES: Autophagy protein 5 (Meiotically up-regulated gene 77 protein) PROTEIN FAMILY: ATG5 family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the ATG5 family. FUNCTION: Involved in cytoplasm to vacuole transport (Cvt) and autophagic vesicle formation. Autophagy is essential for maintenance of amino acid levels and protein synthesis under nitrogen starvation. Required for selective autophagic degradation of the nucleus (nucleophagy). Also required for mitophagy, which eliminates defective or superfluous mitochondria in order to fulfill cellular energy requirements and prevent excess ROS production. Conjugation with atg12, through a ubiquitin-like conjugating system involving atg7 as an E1-like activating enzyme and atg10 as an E2-like conjugating enzyme, is essential for its function. The atg12-atg5 conjugate acts as an E3-like enzyme which is required for lipidation of atg8 and atg8 association to the vesicle membranes (By similarity). Has a role in meiosis and sporulation. KEYWORDS: Autophagy;Cytoplasm;Isopeptide bond;Meiosis;Membrane;Nucleus;Protein transport;Reference proteome;Transport;Ubl conjugation SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Preautophagosomal structure membrane; Peripheral membrane protein. " O75011,"PROTEIN NAMES: Serine/threonine-protein kinase nak1 (N-rich kinase 1) PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. FUNCTION: Has a role in the regulation of cell polarity, growth and division. KEYWORDS: ATP-binding;Cytoplasm;Kinase;Nucleotide-binding;Phosphoprotein;Reference proteome;Serine/threonine-protein kinase;Transferase SUBCELLULAR LOCATION: Cytoplasm " O75072,"PROTEIN NAMES: Ribitol-5-phosphate transferase FKTN (Fukutin) (Fukuyama-type congenital muscular dystrophy protein) (Ribitol-5-phosphate transferase) PROTEIN FAMILY: LicD transferase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the LicD transferase family. FUNCTION: Catalyzes the transfer of a ribitol-phosphate from CDP-ribitol to the distal N-acetylgalactosamine of the phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-3-N-acetylglucosamine-beta-4-(phosphate-6-)mannose), a carbohydrate structure present in alpha-dystroglycan (DAG1). This constitutes the first step in the formation of the ribitol 5-phosphate tandem repeat which links the phosphorylated O-mannosyl trisaccharide to the ligand binding moiety composed of repeats of 3-xylosyl-alpha-1,3-glucuronic acid-beta-1. Required for normal location of POMGNT1 in Golgi membranes, and for normal POMGNT1 activity. May interact with and reinforce a large complex encompassing the outside and inside of muscle membranes. Could be involved in brain development (Probable). PATHWAY: Protein modification; protein glycosylation. KEYWORDS: Alternative splicing;Cardiomyopathy;Congenital muscular dystrophy;Cytoplasm;Disease variant;Dystroglycanopathy;Glycoprotein;Golgi apparatus;Limb-girdle muscular dystrophy;Lissencephaly;Membrane;Nucleus;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus membrane ; Single-pass type II membrane protein Cytoplasm Nucleus Note=In retinal tissue, does not localize with the Golgi apparatus. " O75127,"PROTEIN NAMES: Pentatricopeptide repeat-containing protein 1, mitochondrial PROTEIN FAMILY: PTCD1 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the PTCD1 family. FUNCTION: Mitochondrial protein implicated in negative regulation of leucine tRNA levels, as well as negative regulation of mitochondria-encoded proteins and COX activity. Affects also the 3'-processing of mitochondrial tRNAs. KEYWORDS: Mitochondrion;Reference proteome;Repeat;tRNA processing SUBCELLULAR LOCATION: Mitochondrion Mitochondrion matrix " O75143,"PROTEIN NAMES: Autophagy-related protein 13 PROTEIN FAMILY: ATG13 family, Metazoan subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the ATG13 family. Metazoan subfamily. FUNCTION: Autophagy factor required for autophagosome formation and mitophagy. Target of the TOR kinase signaling pathway that regulates autophagy through the control of the phosphorylation status of ATG13 and ULK1, and the regulation of the ATG13-ULK1-RB1CC1 complex. Through its regulation of ULK1 activity, plays a role in the regulation of the kinase activity of mTORC1 and cell proliferation. KEYWORDS: 3D-structure;Acetylation;Alternative splicing;Autophagy;Cytoplasm;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cytoplasm, cytosol Preautophagosomal structure Note=Under starvation conditions, is localized to puncate structures primarily representing the isolation membrane; the isolation membrane sequesters a portion of the cytoplasm resulting in autophagosome formation. " O75175,"PROTEIN NAMES: CCR4-NOT transcription complex subunit 3 (CCR4-associated factor 3) (Leukocyte receptor cluster member 2) PROTEIN FAMILY: CNOT2/3/5 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the CNOT2/3/5 family. FUNCTION: Component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. May be involved in metabolic regulation; may be involved in recruitment of the CCR4-NOT complex to deadenylation target mRNAs involved in energy metabolism. Involved in mitotic progression and regulation of the spindle assembly checkpoint by regulating the stability of MAD1L1 mRNA. Can repress transcription and may link the CCR4-NOT complex to transcriptional regulation; the repressive function may involve histone deacetylases. Involved in the maintenance of embryonic stem (ES) cell identity. KEYWORDS: 3D-structure;Cytoplasm;Developmental protein;Disease variant;Intellectual disability;Nucleus;Phosphoprotein;Reference proteome;Repressor;RNA-mediated gene silencing;Transcription;Transcription regulation;Translation regulation SUBCELLULAR LOCATION: Cytoplasm Nucleus Cytoplasm, P-body Note=NANOS2 promotes its localization to P-body. " O75204,"PROTEIN NAMES: Transmembrane protein 127 PROTEIN FAMILY: TMEM127 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the TMEM127 family. FUNCTION: Controls cell proliferation acting as a negative regulator of TOR signaling pathway mediated by mTORC1. May act as a tumor suppressor. KEYWORDS: Acetylation;Cell membrane;Cytoplasm;Membrane;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix;Tumor suppressor SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Cytoplasm Note=Association of TMEM127 with the cell membrane is enhanced by inhibition of endocytosis. In the cytoplasm, it colocalizes with markers of early endosomal structures, Golgi apparatus and lysosomes. MISCELLANEOUS: Consistent with the observation that mTORC1 signaling regulates cell growth and size in many species, TMEM127 knockdown cells are larger and proliferate at higher rates compared to control cell lines. In contrast, cell proliferation is reduced in cells overexpressing TMEM127." O75311,"PROTEIN NAMES: Glycine receptor subunit alpha-3 PROTEIN FAMILY: Ligand-gated ion channel family, Glycine receptor subfamily, GLRA3 sub-subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the ligand-gated ion channel (TC 1.A.9) family. Glycine receptor (TC 1.A.9.3) subfamily. GLRA3 sub-subfamily. FUNCTION: Glycine receptors are ligand-gated chloride channels. Channel opening is triggered by extracellular glycine. Channel characteristics depend on the subunit composition; heteropentameric channels display faster channel closure (By similarity). Plays an important role in the down-regulation of neuronal excitability (By similarity). Contributes to the generation of inhibitory postsynaptic currents (By similarity). Contributes to increased pain perception in response to increased prostaglandin E2 levels (By similarity). Plays a role in cellular responses to ethanol (By similarity). KEYWORDS: 3D-structure;Alternative splicing;Cell membrane;Cell projection;Chloride;Chloride channel;Disulfide bond;Glycoprotein;Ion channel;Ion transport;Ligand-gated ion channel;Membrane;Metal-binding;Phosphoprotein;Postsynaptic cell membrane;Receptor;Reference proteome;Signal;Synapse;Transmembrane;Transmembrane helix;Transport;Zinc SUBCELLULAR LOCATION: Postsynaptic cell membrane ; Multi-pass membrane protein Perikaryon Cell projection, dendrite Synapse Cell membrane ; Multi-pass membrane protein Note=Partially colocalizes with GPHN that is known to mediate receptor clustering at postsynaptic membranes. MISCELLANEOUS: The alpha subunit binds strychnine." O75319,"PROTEIN NAMES: RNA/RNP complex-1-interacting phosphatase (Dual specificity protein phosphatase 11) (Phosphatase that interacts with RNA/RNP complex 1) PROTEIN FAMILY: Protein-tyrosine phosphatase family, Non-receptor class dual specificity subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily. FUNCTION: Possesses RNA 5'-triphosphatase and diphosphatase activities, but displays a poor protein-tyrosine phosphatase activity. In addition, has phosphatase activity with ATP, ADP and O-methylfluorescein phosphate (in vitro). Binds to RNA. May participate in nuclear mRNA metabolism. KEYWORDS: 3D-structure;Alternative initiation;Alternative splicing;Hydrolase;Nucleus;Protein phosphatase;Reference proteome;RNA-binding SUBCELLULAR LOCATION: Nucleus Nucleus speckle MISCELLANEOUS: [Isoform 1]: Produced by alternative initiation (Probable). Based on proteomic data (Probable).; MISCELLANEOUS: [Isoform 3]: Major isoform." O75323,PROTEIN NAMES: Protein NipSnap homolog 2 (NipSnap2) (Glioblastoma-amplified sequence) PROTEIN FAMILY: NipSnap family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the NipSnap family. FUNCTION: May act as a positive regulator of L-type calcium channels. KEYWORDS: Alternative splicing;Cytoplasm;Membrane;Mitochondrion;Mitochondrion outer membrane;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Mitochondrion outer membrane O75355,PROTEIN NAMES: Ectonucleoside triphosphate diphosphohydrolase 3 (NTPDase 3) (CD39 antigen-like 3) (Ecto-ATP diphosphohydrolase 3) (Ecto-ATPDase 3) (Ecto-ATPase 3) (Ecto-apyrase 3) (HB6) PROTEIN FAMILY: GDA1/CD39 NTPase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the GDA1/CD39 NTPase family. FUNCTION: Has a threefold preference for the hydrolysis of ATP over ADP. KEYWORDS: Alternative splicing;ATP-binding;Calcium;Cell membrane;Disulfide bond;Glycoprotein;Hydrolase;Magnesium;Membrane;Nucleotide-binding;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein O75360,"PROTEIN NAMES: Homeobox protein prophet of Pit-1 (PROP-1) (Pituitary-specific homeodomain factor) PROTEIN FAMILY: Paired homeobox family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the paired homeobox family. FUNCTION: Possibly involved in the ontogenesis of pituitary gonadotropes, as well as somatotropes, lactotropes and caudomedial thyrotropes. KEYWORDS: Disease variant;DNA-binding;Dwarfism;Homeobox;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus " O75494,"PROTEIN NAMES: Serine/arginine-rich splicing factor 10 (40 kDa SR-repressor protein) (SRrp40) (FUS-interacting serine-arginine-rich protein 1) (Splicing factor SRp38) (Splicing factor, arginine/serine-rich 13A) (TLS-associated protein with Ser-Arg repeats) (TASR) (TLS-associated protein with SR repeats) (TLS-associated serine-arginine protein) (TLS-associated SR protein) PROTEIN FAMILY: Splicing factor SR family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the splicing factor SR family. FUNCTION: Splicing factor that in its dephosphorylated form acts as a general repressor of pre-mRNA splicing. Seems to interfere with the U1 snRNP 5'-splice recognition of SNRNP70. Required for splicing repression in M-phase cells and after heat shock. Also acts as a splicing factor that specifically promotes exon skipping during alternative splicing. Interaction with YTHDC1, a RNA-binding protein that recognizes and binds N6-methyladenosine (m6A)-containing RNAs, prevents SRSF10 from binding to its mRNA-binding sites close to m6A-containing regions, leading to inhibit exon skipping during alternative splicing. May be involved in regulation of alternative splicing in neurons, with isoform 1 acting as a positive and isoform 3 as a negative regulator. KEYWORDS: Alternative splicing;Cytoplasm;mRNA processing;mRNA splicing;Nucleus;Phosphoprotein;Reference proteome;RNA-binding SUBCELLULAR LOCATION: Nucleus speckle Cytoplasm " O75529,"PROTEIN NAMES: TAF5-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 5L (TAF5L) (PCAF-associated factor 65 beta) (PAF65-beta) PROTEIN FAMILY: WD repeat TAF5 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the WD repeat TAF5 family. FUNCTION: Functions as a component of the PCAF complex. The PCAF complex is capable of efficiently acetylating histones in a nucleosomal context. The PCAF complex could be considered as the human version of the yeast SAGA complex (Probable). With TAF6L, acts as an epigenetic regulator essential for somatic reprogramming. Regulates target genes through H3K9ac deposition and MYC recruitment which trigger MYC regulatory network to orchestrate gene expression programs to control embryonic stem cell state (By similarity). KEYWORDS: 3D-structure;Alternative splicing;Direct protein sequencing;Nucleus;Reference proteome;Repeat;Transcription;Transcription regulation;WD repeat SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: [Isoform 2]: May be due to an intron retention." O75534,"PROTEIN NAMES: Cold shock domain-containing protein E1 (N-ras upstream gene protein) (Protein UNR) PROTEIN FAMILY: UNR family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the UNR family. FUNCTION: RNA-binding protein involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. Required for efficient formation of stress granules.; FUNCTION: (Microbial infection) Required for internal initiation of translation of human rhinovirus RNA. KEYWORDS: 3D-structure;Acetylation;Alternative splicing;Cytoplasm;Isopeptide bond;Phosphoprotein;Reference proteome;Repeat;RNA-binding;Ubl conjugation SUBCELLULAR LOCATION: Cytoplasm. Cytoplasm, Stress granule Cytoplasm, P-body " O75553,PROTEIN NAMES: Disabled homolog 1 ORGANISM: Homo sapiens (Human) FUNCTION: Adapter molecule functioning in neural development. May regulate SIAH1 activity. KEYWORDS: Alternative splicing;Developmental protein;Differentiation;Neurodegeneration;Neurogenesis;Phosphoprotein;Reference proteome;Spinocerebellar ataxia O75556,"PROTEIN NAMES: Mammaglobin-B (Lacryglobin) (Lipophilin-C) (Mammaglobin-2) (Secretoglobin family 2A member 1) PROTEIN FAMILY: Secretoglobin family, Lipophilin subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the secretoglobin family. Lipophilin subfamily. FUNCTION: May bind androgens and other steroids, may also bind estramustine, a chemotherapeutic agent used for prostate cancer. May be under transcriptional regulation of steroid hormones. KEYWORDS: Direct protein sequencing;Glycoprotein;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted " O75558,"PROTEIN NAMES: Syntaxin-11 PROTEIN FAMILY: Syntaxin family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the syntaxin family. FUNCTION: SNARE that acts to regulate protein transport between late endosomes and the trans-Golgi network. KEYWORDS: Coiled coil;Familial hemophagocytic lymphohistiocytosis;Golgi apparatus;Membrane;Protein transport;Reference proteome;Transport SUBCELLULAR LOCATION: Membrane ; Peripheral membrane protein Golgi apparatus, trans-Golgi network membrane ; Peripheral membrane protein " O75594,"PROTEIN NAMES: Peptidoglycan recognition protein 1 (Peptidoglycan recognition protein short) (PGRP-S) PROTEIN FAMILY: N-acetylmuramoyl-L-alanine amidase 2 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family. FUNCTION: Innate immunity protein that plays several important functions in antimicrobial and antitumor defense systems. Acts as a pattern receptor that binds to murein peptidoglycans (PGN) of Gram-positive bacteria and thus provides bactericidal activity. Forms an equimolar complex with heat shock protein HSPA1A and induces programmed cell death through apoptosis and necroptosis in tumor cell lines by activating the TNFR1 receptor on the target cell membrane. In addition, acts in complex with the Ca(2+)-binding protein S100A4 as a chemoattractant able to induce lymphocyte movement. Mechanistically, this complex acts as a ligand of the chemotactic receptors CCR5 and CXCR3 which are present on the cells of the immune system. Promotes also the activation of lymphocytes that become able to kill virus-infected cells as well as tumor cells by modulating the spectrum of their target-cell specificity. Induction of cytotoxicity on monocyte surface requires interaction with TREM1 receptor. KEYWORDS: 3D-structure;Antibiotic;Antimicrobial;Disulfide bond;Glycoprotein;Immunity;Innate immunity;Pyrrolidone carboxylic acid;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted Cytoplasmic granule " O75602,"PROTEIN NAMES: Sperm-associated antigen 6 (Protein PF16 homolog) (Repro-SA-1) (Sperm flagellar protein) ORGANISM: Homo sapiens (Human) FUNCTION: Important for structural integrity of the central apparatus in the sperm tail and for flagellar motility. KEYWORDS: Alternative splicing;Cell projection;Cilium;Cilium biogenesis/degradation;Cytoplasm;Cytoskeleton;Flagellum;Microtubule;Reference proteome;Repeat SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton Cell projection, cilium, flagellum Cytoplasm, cytoskeleton, cilium axoneme Note=Associated with microtubules. Detected on the sperm flagellum. Localizes in the cilium axoneme in a SPEF1-dependent manner (By similarity). " O75631,PROTEIN NAMES: Uroplakin-3a (UP3a) (Uroplakin III) (UPIII) PROTEIN FAMILY: Uroplakin-3 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the uroplakin-3 family. FUNCTION: Component of the asymmetric unit membrane (AUM); a highly specialized biomembrane elaborated by terminally differentiated urothelial cells. May play an important role in AUM-cytoskeleton interaction in terminally differentiated urothelial cells. It also contributes to the formation of urothelial glycocalyx which may play an important role in preventing bacterial adherence (By similarity). KEYWORDS: Alternative splicing;Disease variant;Endoplasmic reticulum;Glycoprotein;Membrane;Reference proteome;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Single-pass type I membrane protein Note=Heterodimer formation with UPK1B is a prerequisite to exit out of the endoplasmic reticulum (ER). O75674,"PROTEIN NAMES: TOM1-like protein 1 (Src-activating and signaling molecule protein) (Target of Myb-like protein 1) PROTEIN FAMILY: TOM1 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the TOM1 family. FUNCTION: Probable adapter protein involved in signaling pathways. Interacts with the SH2 and SH3 domains of various signaling proteins when it is phosphorylated. May promote FYN activation, possibly by disrupting intramolecular SH3-dependent interactions (By similarity). KEYWORDS: 3D-structure;Alternative splicing;Cytoplasm;Endosome;Golgi apparatus;Membrane;Phosphoprotein;Protein transport;Reference proteome;SH3-binding;Transport SUBCELLULAR LOCATION: Golgi apparatus, Golgi stack. Endosome membrane Cytoplasm Membrane ; Peripheral membrane protein ; Cytoplasmic side Note=A small proportion is membrane-associated. " O75678,"PROTEIN NAMES: Ret finger protein-like 2 (RING finger protein 79) ORGANISM: Homo sapiens (Human) KEYWORDS: Alternative splicing;Metal-binding;Reference proteome;Zinc;Zinc-finger MISCELLANEOUS: [Isoform 2]: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.; MISCELLANEOUS: [Isoform 3]: May be due to intron retention." O75751,"PROTEIN NAMES: Solute carrier family 22 member 3 (Extraneuronal monoamine transporter) (EMT) (Organic cation transporter 3) (OCT3) PROTEIN FAMILY: Major facilitator superfamily, Organic cation transporter family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the major facilitator (TC 2.A.1) superfamily. Organic cation transporter (TC 2.A.1.19) family. FUNCTION: Electrogenic voltage-dependent transporter that mediates the transport of a variety of organic cations such as endogenous bioactive amines, cationic drugs and xenobiotics. Cation cellular uptake or release is driven by the electrochemical potential, i.e. membrane potential and concentration gradient. Functions as a Na(+)- and Cl(-)-independent, bidirectional uniporter. Implicated in monoamine neurotransmitters uptake such as dopamine, adrenaline/epinephrine, noradrenaline/norepinephrine, histamine, serotonin and tyramine, thereby supporting a role in homeostatic regulation of aminergic neurotransmission in the brain. Transports dopaminergic neuromodulators cyclo(his-pro) and salsolinol with low efficiency. May be involved in the uptake and disposition of cationic compounds by renal clearance from the blood flow. May contribute to regulate the transport of cationic compounds in testis across the blood-testis-barrier (Probable). Mediates the transport of polyamine spermidine and putrescine (By similarity). Mediates the bidirectional transport of polyamine agmatine. Also transports guanidine. May also mediate intracellular transport of organic cations, thereby playing a role in amine metabolism and intracellular signaling (By similarity). KEYWORDS: 3D-structure;Cell membrane;Glycoprotein;Ion transport;Membrane;Mitochondrion;Nucleus;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Apical cell membrane ; Multi-pass membrane protein Basolateral cell membrane ; Multi-pass membrane protein Mitochondrion membrane Endomembrane system Nucleus membrane Nucleus outer membrane Note=Localized to the apical/brush border membrane of enterocytes. Localized to the luminal/apical membrane of ciliated epithelial cells in bronchi. Localized to the basolateral membrane of intermediate cells in bronchi. Localized to the entire plasma membrane of basal cells in bronchi. MISCELLANEOUS: Mediates the uptake of clinically used drugs including neurotoxin 1-methyl-4-phenylpyridinium (MPP(+)) and platinum-based drug oxaliplatin. Plays a role in the anticancer activity of oxaliplatin and may contribute to antitumor specificity." O75752,"PROTEIN NAMES: UDP-GalNAc:beta-1,3-N-acetylgalactosaminyltransferase 1 (Beta-1,3-GalNAc-T1) (Beta-1,3-galactosyltransferase 3) (Beta-1,3-GalTase 3) (Beta3Gal-T3) (Beta3GalT3) (b3Gal-T3) (Beta-3-Gx-T3) (Galactosylgalactosylglucosylceramide beta-D-acetyl-galactosaminyltransferase) (Globoside synthase) (UDP-N-acetylgalactosamine:globotriaosylceramide beta-1,3-N-acetylgalactosaminyltransferase) PROTEIN FAMILY: Glycosyltransferase 31 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the glycosyltransferase 31 family. FUNCTION: Transfers N-acetylgalactosamine onto globotriaosylceramide. Plays a critical role in preimplantation stage embryonic development (By similarity). PATHWAY: Protein modification; protein glycosylation. KEYWORDS: Blood group antigen;Glycoprotein;Glycosyltransferase;Golgi apparatus;Lipid metabolism;Magnesium;Membrane;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus membrane; Single-pass type II membrane protein. " O75791,PROTEIN NAMES: GRB2-related adapter protein 2 (Adapter protein GRID) (GRB-2-like protein) (GRB2L) (GRBLG) (GRBX) (Grf40 adapter protein) (Grf-40) (Growth factor receptor-binding protein) (Hematopoietic cell-associated adapter protein GrpL) (P38) (Protein GADS) (SH3-SH2-SH3 adapter Mona) PROTEIN FAMILY: GRB2/sem-5/DRK family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the GRB2/sem-5/DRK family. FUNCTION: Interacts with SLP-76 to regulate NF-AT activation. Binds to tyrosine-phosphorylated shc. KEYWORDS: 3D-structure;Acetylation;Alternative splicing;Cytoplasm;Endosome;Nucleus;Phosphoprotein;Reference proteome;Repeat;SH2 domain;SH3 domain SUBCELLULAR LOCATION: Nucleus Cytoplasm Endosome O75817,"PROTEIN NAMES: Ribonuclease P protein subunit p20 (RNaseP protein p20) (Ribonucleases P/MRP protein subunit POP7 homolog) (hPOP7) PROTEIN FAMILY: Histone-like Alba family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the histone-like Alba family. FUNCTION: Component of ribonuclease P, a ribonucleoprotein complex that generates mature tRNA molecules by cleaving their 5'-ends. Also a component of the MRP ribonuclease complex, which cleaves pre-rRNA sequences. KEYWORDS: 3D-structure;Cytoplasm;Direct protein sequencing;Nucleus;Reference proteome;RNA-binding;rRNA processing;tRNA processing SUBCELLULAR LOCATION: Nucleus, nucleolus Cytoplasm Cytoplasmic granule Note=Under stress conditions colocalizes with SMN1 in punctuated cytoplasmic granules. " O75821,"PROTEIN NAMES: Eukaryotic translation initiation factor 3 subunit G (eIF3g) (Eukaryotic translation initiation factor 3 RNA-binding subunit) (eIF-3 RNA-binding subunit) (Eukaryotic translation initiation factor 3 subunit 4) (eIF-3-delta) (eIF3 p42) (eIF3 p44) PROTEIN FAMILY: EIF-3 subunit G family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the eIF-3 subunit G family. FUNCTION: RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression. This subunit can bind 18S rRNA.; FUNCTION: (Microbial infection) In case of FCV infection, plays a role in the ribosomal termination-reinitiation event leading to the translation of VP2. KEYWORDS: 3D-structure;Cytoplasm;Direct protein sequencing;Initiation factor;Nucleus;Phosphoprotein;Protein biosynthesis;Reference proteome;RNA-binding SUBCELLULAR LOCATION: Cytoplasm Nucleus Cytoplasm, perinuclear region Note=Colocalizes with AIFM1 in the nucleus and perinuclear region. " O75840,"PROTEIN NAMES: Krueppel-like factor 7 (Ubiquitous krueppel-like factor) PROTEIN FAMILY: Krueppel C2H2-type zinc-finger protein family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein family. FUNCTION: Transcriptional factor. Plays a critical role in neuronal morphogenesis and survival of sensory neurons (By similarity). Represses the corneal epithelium differentiation. Acts also as a metabolic regulator, by modulating insulin sensitivity in pancreatic beta cells and skeletal muscle cells. Inhibits transcriptional inducers of adipogenesis and has a repressive role in the expression of several adipokines, including leptin. KEYWORDS: Activator;Alternative splicing;Disease variant;DNA-binding;Metal-binding;Nucleus;Reference proteome;Repeat;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus " O75865,"PROTEIN NAMES: Trafficking protein particle complex subunit 6A (TRAPP complex subunit 6A) PROTEIN FAMILY: TRAPP small subunits family, BET3 subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the TRAPP small subunits family. BET3 subfamily. FUNCTION: May play a role in vesicular transport during the biogenesis of melanosomes. KEYWORDS: 3D-structure;Alternative splicing;Endoplasmic reticulum;ER-Golgi transport;Golgi apparatus;Phosphoprotein;Reference proteome;Transport SUBCELLULAR LOCATION: Golgi apparatus, cis-Golgi network Endoplasmic reticulum " O75879,"PROTEIN NAMES: Glutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrial (Glu-AdT subunit B) (Cytochrome c oxidase assembly factor PET112 homolog) PROTEIN FAMILY: GatB/GatE family, GatB subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the GatB/GatE family. GatB subfamily. FUNCTION: Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln). KEYWORDS: ATP-binding;Ligase;Mitochondrion;Nucleotide-binding;Primary mitochondrial disease;Protein biosynthesis;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Mitochondrion " O75915,"PROTEIN NAMES: PRA1 family protein 3 (ADP-ribosylation factor-like protein 6-interacting protein 5) (ARL-6-interacting protein 5) (Aip-5) (Cytoskeleton-related vitamin A-responsive protein) (Dermal papilla-derived protein 11) (GTRAP3-18) (Glutamate transporter EAAC1-interacting protein) (JM5) (Prenylated Rab acceptor protein 2) (Protein JWa) (Putative MAPK-activating protein PM27) PROTEIN FAMILY: PRA1 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the PRA1 family. FUNCTION: Regulates intracellular concentrations of taurine and glutamate. Negatively modulates SLC1A1/EAAC1 glutamate transport activity by decreasing its affinity for glutamate in a PKC activity-dependent manner. Plays a role in the retention of SLC1A1/EAAC1 in the endoplasmic reticulum. KEYWORDS: Acetylation;Cell membrane;Cytoplasm;Cytoskeleton;Direct protein sequencing;Endoplasmic reticulum;Membrane;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein Cell membrane ; Multi-pass membrane protein Cytoplasm Cytoplasm, cytoskeleton Note=Also exists as a soluble form in the cytoplasm. Associated with microtubules. " O75920,"PROTEIN NAMES: Small EDRK-rich factor 1 (Protein 4F5) (h4F5) (SMA modifier 1) PROTEIN FAMILY: SERF family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the SERF family. FUNCTION: Positive regulator of amyloid protein aggregation and proteotoxicity. Induces conformational changes in amyloid proteins, such as APP, HTT, and SNCA, driving them into compact formations preceding the formation of aggregates. KEYWORDS: Alternative splicing;Cytoplasm;Nucleus;Reference proteome SUBCELLULAR LOCATION: Cytoplasm, cytosol Nucleus " O75925,"PROTEIN NAMES: E3 SUMO-protein ligase PIAS1 (DEAD/H box-binding protein 1) (E3 SUMO-protein transferase PIAS1) (Gu-binding protein) (GBP) (Protein inhibitor of activated STAT protein 1) (RNA helicase II-binding protein) PROTEIN FAMILY: PIAS family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the PIAS family. FUNCTION: Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Catalyzes sumoylation of various proteins, such as CEBPB, MRE11, MTA1, PTK2 and PML. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. In vitro, binds A/T-rich DNA. The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context. Mediates sumoylation of MRE11, stabilizing MRE11 on chromatin during end resection. Sumoylates PML (at 'Lys-65' and 'Lys-160') and PML-RAR and promotes their ubiquitin-mediated degradation (By similarity). PIAS1-mediated sumoylation of PML promotes its interaction with CSNK2A1/CK2 which in turn promotes PML phosphorylation and degradation (By similarity). Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation. Plays a dynamic role in adipogenesis by promoting the SUMOylation and degradation of CEBPB (By similarity). Mediates the nuclear mobility and localization of MSX1 to the nuclear periphery, whereby MSX1 is brought into the proximity of target myoblast differentiation factor genes (By similarity). Also required for the binding of MSX1 to the core enhancer region in target gene promoter regions, independent of its sumoylation activity (By similarity). Capable of binding to the core enhancer region TAAT box in the MYOD1 gene promoter (By similarity).; FUNCTION: (Microbial infection) Restricts Epstein-Barr virus (EBV) lytic replication by acting as an inhibitor for transcription factors involved in lytic gene expression. The virus can use apoptotic caspases to antagonize PIAS1-mediated restriction and express its lytic genes. PATHWAY: Protein modification; protein sumoylation. KEYWORDS: 3D-structure;Acetylation;Alternative splicing;Cytoplasm;Cytoskeleton;DNA-binding;Host-virus interaction;Isopeptide bond;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Repeat;Transcription;Transcription regulation;Transferase;Ubl conjugation;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Nucleus speckle Nucleus, PML body Cytoplasm, cytoskeleton Note=Interaction with CSRP2 may induce a partial redistribution along the cytoskeleton. Interaction with MSX1 is required for localization to the nuclear periphery (By similarity). " O75937,PROTEIN NAMES: DnaJ homolog subfamily C member 8 (Splicing protein spf31) ORGANISM: Homo sapiens (Human) FUNCTION: Suppresses polyglutamine (polyQ) aggregation of ATXN3 in neuronal cells. KEYWORDS: 3D-structure;Acetylation;Chaperone;Nucleus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Nucleus O75940,"PROTEIN NAMES: Survival of motor neuron-related-splicing factor 30 (30 kDa splicing factor SMNrp) (SMN-related protein) (Survival motor neuron domain-containing protein 1) PROTEIN FAMILY: SMN family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the SMN family. FUNCTION: Involved in spliceosome assembly. KEYWORDS: 3D-structure;Acetylation;Apoptosis;Direct protein sequencing;mRNA processing;mRNA splicing;Nucleus;Phosphoprotein;Reference proteome;Spliceosome SUBCELLULAR LOCATION: Nucleus speckle Nucleus, Cajal body Note=Detected in nuclear speckles containing snRNP and in Cajal (coiled) bodies. " O76021,"PROTEIN NAMES: Ribosomal L1 domain-containing protein 1 (CATX-11) (Cellular senescence-inhibited gene protein) (Protein PBK1) PROTEIN FAMILY: Universal ribosomal protein uL1 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the universal ribosomal protein uL1 family. Highly divergent. FUNCTION: Regulates cellular senescence through inhibition of PTEN translation. Acts as a pro-apoptotic regulator in response to DNA damage. KEYWORDS: 3D-structure;Acetylation;Alternative splicing;Coiled coil;Direct protein sequencing;Isopeptide bond;Nucleus;Phosphoprotein;Reference proteome;Ubl conjugation SUBCELLULAR LOCATION: Nucleus, nucleolus Note=Colocalizes with ING1 in the nucleolus after UV stress. " O76027,PROTEIN NAMES: Annexin A9 (Annexin XXXI) (Annexin-31) (Annexin-9) (Pemphaxin) PROTEIN FAMILY: Annexin family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the annexin family. FUNCTION: Low affinity receptor for acetylcholine known to be targeted by disease-causing pemphigus vulgaris antibodies in keratinocytes. KEYWORDS: Annexin;Reference proteome;Repeat O76050,"PROTEIN NAMES: E3 ubiquitin-protein ligase NEURL1 (Neuralized-like protein 1A) (h-neu) (h-neuralized 1) (RING finger protein 67) (RING-type E3 ubiquitin transferase NEURL1) ORGANISM: Homo sapiens (Human) FUNCTION: Plays a role in hippocampal-dependent synaptic plasticity, learning and memory. Involved in the formation of spines and functional synaptic contacts by modulating the translational activity of the cytoplasmic polyadenylation element-binding protein CPEB3. Promotes ubiquitination of CPEB3, and hence induces CPEB3-dependent mRNA translation activation of glutamate receptor GRIA1 and GRIA2. Can function as an E3 ubiquitin-protein ligase to activate monoubiquitination of JAG1 (in vitro), thereby regulating the Notch pathway. Acts as a tumor suppressor; inhibits malignant cell transformation of medulloblastoma (MB) cells by inhibiting the Notch signaling pathway. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Alternative splicing;Cell membrane;Cell projection;Cytoplasm;Lipoprotein;Membrane;Metal-binding;Myristate;Notch signaling pathway;Reference proteome;Repeat;Synapse;Transferase;Translation regulation;Tumor suppressor;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cytoplasm, perinuclear region Cell membrane ; Peripheral membrane protein Perikaryon Cell projection, dendrite Postsynaptic density Note=Localized in the cell bodies of the pyramidal neurons and distributed along their apical dendrites. Colocalized with PSD95 in postsynaptic sites. Colocalized with CPEB3 at apical dendrites of CA1 neurons (By similarity). Colocalized with JAG1 at the cell surface. " O76074,"PROTEIN NAMES: cGMP-specific 3',5'-cyclic phosphodiesterase (cGMP-binding cGMP-specific phosphodiesterase) (CGB-PDE) PROTEIN FAMILY: Cyclic nucleotide phosphodiesterase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the cyclic nucleotide phosphodiesterase family. FUNCTION: Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This phosphodiesterase catalyzes the specific hydrolysis of cGMP to 5'-GMP. Specifically regulates nitric-oxide-generated cGMP. PATHWAY: Purine metabolism; 3',5'-cyclic GMP degradation; GMP from 3',5'-cyclic GMP: step 1/1. KEYWORDS: 3D-structure;Allosteric enzyme;Alternative splicing;cGMP;cGMP-binding;Hydrolase;Metal-binding;Nucleotide-binding;Phosphoprotein;Reference proteome;Repeat;Zinc " O76337,PROTEIN NAMES: Cell death protein 6 (Candidate adapter protein ced-6) PROTEIN FAMILY: Ced-6 family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the ced-6 family. FUNCTION: May function as an adapter protein in a pathway that mediates recognition and phagocytosis of apoptotic cells during normal development. Promotes engulfment of cells at both early and late stages of apoptosis. Required for actin reorganization around apoptotic cells. Plays a role in protecting dopaminergic neurons from oxidative stress-induced degeneration. Mediates recruitment of E3 ubiquitin-protein ligase trim-21 to the apoptotic cell surface which promotes ubiquitination and degradation of ced-1. KEYWORDS: Apoptosis;Cytoplasm;Developmental protein;Phagocytosis;Reference proteome SUBCELLULAR LOCATION: Cytoplasm O76360,"PROTEIN NAMES: cGMP-dependent protein kinase egl-4 (Egg-laying defective protein 4) PROTEIN FAMILY: Protein kinase superfamily, AGC Ser/Thr protein kinase family, cGMP subfamily ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cGMP subfamily. FUNCTION: Promotes chemoreceptor gene expression in response to increased cGMP levels by antagonizing the gene repression functions of the class II HDAC hda-4 and the mef-2 transcription factor. Regulates gene expression via recruitment of a histone deacetylase complex containing hda-2, saeg-1 and saeg-2. Represses body size and lifespan through the dbl-1 and insulin pathways, respectively. May also signal through daf-3 and/or daf-5. Role in egg-laying, dauer formation and motility. Regulates behavioral responses to various chemosensory stimuli in sensory neurons. Required for the initiation of long term adaptation to prolonged odor exposure which results in a decrease in odor seeking behavior. May regulate this process by phosphorylating tax-2, a subunit of cyclic nucleotide-gated channel tax-2/tax-4. In ASH sensory neurons, negatively regulates avoidance behavior to some bitter tastants, such as quinine, probably by phosphorylating rgs-2 and rgs-3 which are 2 regulator of G-protein signaling proteins. In AWB sensory neurons, involved in avoidance behavior to some repellent odors. In ASE left (ASEL) sensory neuron, involved in the sensing of environmental alkalinity downstream of receptor-type guanylate cyclase gcy-14. In sensory neurons, involved in the signaling pathway downstream of insulin, TGF-beta and receptor-type guanylate cyclase responsible for inducing quiescence after food intake. Might play a role in aversive olfactory learning in AWC neurons when an odor is associated with food deprivation, depending on the ins-1/age-1 signal from the AIA to the AWC neurons. Probably by regulating neuronal transmission downstream of lin-3 and receptor lin-23 and phospholipase plc-3 in ALA neurons, involved in the decrease in locomotion during the quiescent state that precedes each larval molt. KEYWORDS: Alternative splicing;ATP-binding;cGMP;cGMP-binding;Chemotaxis;Coiled coil;Cytoplasm;Developmental protein;Kinase;Magnesium;Metal-binding;Nucleotide-binding;Nucleus;Reference proteome;Repeat;Serine/threonine-protein kinase;Transferase SUBCELLULAR LOCATION: Cytoplasm Nucleus Note=In resting AWC sensory neurons, localizes in cytoplasm. Prolonged exposure to attractive odorants sensed by AWC neurons results in nuclear translocation. Nuclear translocation is required for the adaptation to prolonged odor exposure and is controlled by G(o)-alpha subunit protein goa-1. Localization is regulated by cGMP levels: high cGMP levels result in cytoplasmic localization whereas low cGMP levels result in nuclear localization. Nuclear localization in AWC neurons is dependent on age-1. In addition, an intact sensory cilia structure is required for cytoplasmic localization in resting AWC neurons. In resting AWB sensory neurons, constitutive nuclear localization is dependent on goa-1. In resting ASH sensory neurons, localizes in both cytoplasm and nucleus. Cytoplasmic localization is important for negative regulation of quinine sensitivity in ASH neurons. " O76689,"PROTEIN NAMES: Sodium-dependent acetylcholine transporter (Sodium:neurotransmitter symporter family protein 6) PROTEIN FAMILY: Sodium:neurotransmitter symporter (SNF) family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. FUNCTION: Mediates sodium-dependent uptake of acetylcholine at neuromuscular junctions during periods of increased synaptic activity, may also prevent spillover to adjacent synaptic sites. Not involved in the uptake of other neurotransmitters (GABA, glycine, proline and glutamate) and there was also no inhibition of uptake by adding an excess of other candidate substrates (GABA, glycine, taurine, creatine, proline, alanine, carnitine, glutamate and betaine). Required for muscle integrity; altered transport of acetylcholine due to loss of dystrophin-glycoprotein complex (DGC) function results in muscle degeneration. KEYWORDS: Cell membrane;Glycoprotein;Membrane;Neurotransmitter transport;Postsynaptic cell membrane;Reference proteome;Symport;Synapse;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Postsynaptic cell membrane ; Multi-pass membrane protein Note=The DGC is not necessary to establish snf-6 at the postsynaptic membrane, but it is required for maintenance or stabilization. " O76745,"PROTEIN NAMES: Nitrophorin Cim l NP (NP) (Salivary nitrophorin) (cNP) (allergen Cim l NP) ORGANISM: Cimex lectularius (Bed bug) (Acanthia lectularia) FUNCTION: Heme-based protein that delivers nitric oxide gas (NO) to the victim while feeding, resulting in vasodilation (Probable). In place of heme, the heme-binding cysteine can also reversibly bind NO when it is present in high concentrations. KEYWORDS: 3D-structure;Allergen;Direct protein sequencing;Heme;Iron;Metal-binding;Reference proteome;Secreted;Signal;Transport;Vasoactive;Vasodilator SUBCELLULAR LOCATION: Secreted MISCELLANEOUS: Has some sequence similarity to inositol phosphatases, but no inositol trisphosphate phosphatase activity is detected." O76924,"PROTEIN NAMES: Potential E3 ubiquitin-protein ligase ariadne-2 (Protein ariadne-2) (RING-type E3 ubiquitin transferase ariadne-2) PROTEIN FAMILY: RBR family, Ariadne subfamily ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the RBR family. Ariadne subfamily. FUNCTION: Might act as an E3 ubiquitin-protein ligase, or as part of E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes and then transfers it to substrates. KEYWORDS: Coiled coil;Metal-binding;Nucleus;Reference proteome;Repeat;Transferase;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: Overexpression does not rescue clustering of muscle nuceli in ari-1 mutants." O77237,PROTEIN NAMES: Protein pellino PROTEIN FAMILY: Pellino family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the pellino family. FUNCTION: Scaffold protein involved in the Toll signaling pathway via its interaction with pelle/pll kinase. KEYWORDS: Phosphoprotein;Reference proteome O77459,PROTEIN NAMES: Transcription factor Ken (Protein Ken and Barbie) ORGANISM: Drosophila melanogaster (Fruit fly) FUNCTION: Transcription factor required for terminalia development. Negative regulator of the JAK/STAT pathway: represses JAK/STAT-dependent expression of ventral veins lacking (vvl) in the posterior spiracles. KEYWORDS: Developmental protein;DNA-binding;Metal-binding;Nucleus;Reference proteome;Repeat;Repressor;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus O77726,"PROTEIN NAMES: Zona pellucida sperm-binding protein 2 (Zona pellucida glycoprotein 2) (Zp-2) (Zona pellucida protein A) [Cleaved into: Processed zona pellucida sperm-binding protein 2] PROTEIN FAMILY: ZP domain family, ZPA subfamily ORGANISM: Macaca radiata (Bonnet macaque) SIMILARITY: Belongs to the ZP domain family. ZPA subfamily. FUNCTION: Component of the zona pellucida, an extracellular matrix surrounding oocytes which mediates sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy. The zona pellucida is composed of 3 to 4 glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP2 may act as a secondary sperm receptor. KEYWORDS: Cell membrane;Cleavage on pair of basic residues;Disulfide bond;Extracellular matrix;Fertilization;Glycoprotein;Membrane;Receptor;Secreted;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: [Processed zona pellucida sperm-binding protein 2]: Zona pellucida.; SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein " O77760,"PROTEIN NAMES: Sterol O-acyltransferase 1 (Acyl-coenzyme A:cholesterol acyltransferase 1) (ACAT-1) (Cholesterol acyltransferase 1) PROTEIN FAMILY: Membrane-bound acyltransferase family, Sterol o-acyltransferase subfamily ORGANISM: Chlorocebus aethiops (Green monkey) (Cercopithecus aethiops) SIMILARITY: Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily. FUNCTION: Catalyzes the formation of fatty acid-cholesterol esters, which are less soluble in membranes than cholesterol. Plays a role in lipoprotein assembly and dietary cholesterol absorption. Utilizes oleoyl-CoA ((9Z)-octadecenoyl-CoA) preferentially as susbstrate: shows a higher activity towards an acyl-CoA substrate with a double bond at the delta-9 position (9Z) than towards saturated acyl-CoA or an unsaturated acyl-CoA with a double bond at the delta-7 (7Z) or delta-11 (11Z) positions. KEYWORDS: Acetylation;Acyltransferase;Cholesterol metabolism;Disulfide bond;Endoplasmic reticulum;Lipid metabolism;Membrane;Phosphoprotein;Steroid metabolism;Sterol metabolism;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein " O80366,"PROTEIN NAMES: Two-component response regulator ARR9 (Response reactor 4) PROTEIN FAMILY: ARR family, Type-A subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the ARR family. Type-A subfamily. FUNCTION: Functions as a response regulator involved in His-to-Asp phosphorelay signal transduction system. Phosphorylation of the Asp residue in the receiver domain activates the ability of the protein to promote the transcription of target genes. Type-A response regulators seem to act as negative regulators of the cytokinin signaling. KEYWORDS: Cytokinin signaling pathway;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation;Two-component regulatory system SUBCELLULAR LOCATION: Nucleus " O80458,"PROTEIN NAMES: Dehydrodolichyl diphosphate synthase 1 (Dedol-PP synthase 1) (Ditrans,polycis-polyprenyl diphosphate synthase ((2E,6E)-farnesyl diphosphate specific) 1) PROTEIN FAMILY: UPP synthase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the UPP synthase family. FUNCTION: Catalyzes cis-prenyl chain elongation to produce the polyprenyl backbone of dolichol, a glycosyl carrier-lipid required for the biosynthesis of several classes of glycoprotein. PATHWAY: Protein modification; protein glycosylation. KEYWORDS: Endoplasmic reticulum;Membrane;Reference proteome;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Single-pass membrane protein " O80528,"PROTEIN NAMES: Serine/threonine-protein kinase haspin homolog (AtHaspin) PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family, Haspin subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Haspin subfamily. FUNCTION: Threonine-protein kinase that phosphorylates histone H3 in vitro at 'Thr-3' (H3T3ph) and 'Thr-11' (H3T11ph), but not at 'Ser-10' (H3S10ph) or 'Ser-28' (H3S28ph). Plays a role in mitotic cell division during plant growth. Threonine-protein kinase that phosphorylates histone H3 in vitro at 'Thr-3' (H3T3ph), but not at 'Thr-11' (H3T11ph), 'Ser-10' (H3S10ph) or 'Ser-28' (H3S28ph). Involved in histone H3 phosphorylation in mitotic cells. Contributes to organ and plant development, as well as embryonic patterning. KEYWORDS: ATP-binding;Cell cycle;Chromosome;Cytoplasm;Cytoskeleton;Kinase;Nucleotide-binding;Nucleus;Reference proteome;Serine/threonine-protein kinase;Transferase SUBCELLULAR LOCATION: Cytoplasm Cytoplasm, perinuclear region Nucleus Chromosome Cytoplasm, cytoskeleton, phragmoplast Note=During interphase, localized in the cytoplasm and at the nuclear periphery. During prometaphase and metaphase, localized on chromosomes, and around the cell plate during cytokinesis. MISCELLANEOUS: Over-expression of an inactive kinase mutant decreases the size of the root meristem and delays root growth." O80548,"PROTEIN NAMES: MA3 DOMAIN-CONTAINING TRANSLATION REGULATORY FACTOR 2 (MA3 domain-containing protein 8) PROTEIN FAMILY: PDCD4 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the PDCD4 family. FUNCTION: Involved in target of rapamycin (TOR)-regulated translation control, especially under energy-deficient conditions. KEYWORDS: Cytoplasm;Nucleus;Reference proteome;Repeat;Translation regulation SUBCELLULAR LOCATION: Nucleus Cytoplasm, cytosol " O80612,PROTEIN NAMES: Probable apyrase 6 (AtAPY6) (ATP-diphosphatase) (ATP-diphosphohydrolase) (Adenosine diphosphatase) (ADPase) (NTPDase) (Nucleoside triphosphate diphosphohydrolase 6) PROTEIN FAMILY: GDA1/CD39 NTPase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the GDA1/CD39 NTPase family. FUNCTION: Catalyzes the hydrolysis of phosphoanhydride bonds of nucleoside tri- and di-phosphates (By similarity). Involved in the regulation of pollen and anther development. KEYWORDS: ATP-binding;Calcium;Cytoplasmic vesicle;Glycoprotein;Hydrolase;Membrane;Nucleotide-binding;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cytoplasmic vesicle membrane ; Multi-pass membrane protein O80642,PROTEIN NAMES: Geranylgeranyl transferase type-1 subunit beta (Geranylgeranyl transferase type I subunit beta) (AtGGT-IB) (GGTase-I-beta) PROTEIN FAMILY: Protein prenyltransferase subunit beta family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the protein prenyltransferase subunit beta family. FUNCTION: Catalyzes the transfer of a geranyl-geranyl moiety from geranyl-geranyl pyrophosphate to a cysteine at the fourth position from the C-terminus of proteins having the C-terminal sequence Cys-aliphatic-aliphatic-X (CaaX). Seems to exclusively prenylate CaaX substrates with leucine in the terminal position. The beta subunit is responsible for peptide-binding. May negatively regulate abscisic acid (ABA) signaling in guard cells and auxin-induced lateral root initiation.; FUNCTION: Negatively regulates ABA signaling in guard cells. in negative regulation of auxin-induced lateral root initiation. KEYWORDS: Acetylation;Magnesium;Metal-binding;Prenyltransferase;Reference proteome;Repeat;Transferase;Zinc O80809,"PROTEIN NAMES: Receptor-like protein CLAVATA2 (Receptor-like protein 10) (AtRLP10) PROTEIN FAMILY: RLP family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the RLP family. FUNCTION: Involved in the perception of CLV3 and CLV3-like (CLE) peptides, that act as extracellular signals regulating meristems maintenance. Required for the sensing of the root CLE peptides (e.g. CLE8, CLE9/CLE10, CLE11, CLE13, CLE14, CLE16, CLE17, CLE18, CLE20, CLE21, CLE25, CLE26, CLE40, CLE41/CLE44 and CLE45), which involves also CRN and leads to root growth regulation, mostly in the phloem and protophloem. Involved in controlling the stem cell population size in shoot and root apical meristems, and during organ development. Promotes the formation of CLV1 multimers. In complex with CRN, perceives secreted CLV3-like effector proteins from plant-parasitic cyst nematodes as ligand mimics of the plant CLE signaling pathway. This recognition is required for proper feeding structure (syncytium) development and ultimately successful nematode infection. CLE14 perception by CLV2/CRN complex triggers root meristem differentiation. KEYWORDS: Cell membrane;Developmental protein;Disulfide bond;Endoplasmic reticulum;Glycoprotein;Leucine-rich repeat;Membrane;Receptor;Reference proteome;Repeat;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein Endoplasmic reticulum membrane ; Single-pass type I membrane protein Note=Requires CRN for export from the endoplasmic reticulum and localization to the plasma membrane. " O80871,PROTEIN NAMES: Probable protein phosphatase 2C 25 (AtPP2C25) (Protein phosphatase AP2C1) PROTEIN FAMILY: PP2C family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the PP2C family. FUNCTION: Protein phosphatase that negatively regulates defense respones. Inactivates MPK4 and MPK6 MAP kinases involved in stress and defense signaling. KEYWORDS: Cytoplasm;Hydrolase;Magnesium;Manganese;Metal-binding;Nucleus;Protein phosphatase;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Nucleus O80888,"PROTEIN NAMES: Mitogen-activated protein kinase kinase kinase 17 PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. FUNCTION: Component of the abscisic acid (ABA) signaling pathway that may act as ABA signal transducer in the context of abiotic stresses. Triggers MPK7 activation in a MKK3-dependent manner. Mediates the ABA-dependent activation of the MKK3-MPK7 module. KEYWORDS: Abscisic acid signaling pathway;ATP-binding;Kinase;Nucleotide-binding;Nucleus;Phosphoprotein;Reference proteome;Serine/threonine-protein kinase;Transferase SUBCELLULAR LOCATION: Nucleus " O81833,"PROTEIN NAMES: G-type lectin S-receptor-like serine/threonine-protein kinase SD1-1 (S-domain-1 (SD1) receptor kinase 1) (SD1-1) PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. KEYWORDS: ATP-binding;Cell membrane;Disulfide bond;EGF-like domain;Glycoprotein;Kinase;Lectin;Membrane;Nucleotide-binding;Phosphoprotein;Receptor;Reference proteome;Serine/threonine-protein kinase;Signal;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein " O81893,"PROTEIN NAMES: Inositol-tetrakisphosphate 1-kinase 2 (Inositol 1,3,4-trisphosphate 5/6-kinase 2) (AtItpk-2) (Inositol-triphosphate 5/6-kinase 2) (Ins(1,3,4)P(3) 5/6-kinase 2) PROTEIN FAMILY: ITPK1 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the ITPK1 family. FUNCTION: Kinase that can phosphorylate various inositol polyphosphate such as Ins(3,4,5,6)P4 or Ins(1,3,4)P3. Phosphorylates Ins(3,4,5,6)P4 to form InsP5. This reaction is thought to have regulatory importance, since Ins(3,4,5,6)P4 is an inhibitor of plasma membrane Ca(2+)-activated Cl(-) channels, while Ins(1,3,4,5,6)P5 is not (By similarity). Also phosphorylates Ins(1,3,4)P3 or a racemic mixture of Ins(1,4,6)P3 and Ins(3,4,6)P3 to form InsP4. Ins(1,3,4,6)P4 is an essential molecule in the hexakisphosphate (InsP6) pathway (By similarity). Plays a role in seed coat development and lipid polyester barrier formation. KEYWORDS: Alternative splicing;ATP-binding;Kinase;Magnesium;Metal-binding;Nucleotide-binding;Reference proteome;Transferase " O82132,"PROTEIN NAMES: Dehydration-responsive element-binding protein 2A (Protein DREB2A) PROTEIN FAMILY: AP2/ERF transcription factor family, ERF subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the AP2/ERF transcription factor family. ERF subfamily. FUNCTION: Transcriptional activator that binds specifically to the DNA sequence 5'-[AG]CCGAC-3'. Binding to the C-repeat/DRE element mediates high salinity- and dehydration-inducible transcription. Promotes the expression of heat stress-inducible genes by contributing to the formation of a heat stress-specific transcriptional complex with NF-Y subunits (e.g. DPB3-1, NF-YA2 and NF-YB3) at the promoter of target genes, thus promoting heat tolerance. KEYWORDS: 3D-structure;Activator;Alternative splicing;DNA-binding;Nucleus;Reference proteome;Stress response;Transcription;Transcription regulation;Ubl conjugation SUBCELLULAR LOCATION: Nucleus " O82162,"PROTEIN NAMES: Eyes absent homolog (AtEYA) PROTEIN FAMILY: HAD-like hydrolase superfamily, EYA family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the HAD-like hydrolase superfamily. EYA family. FUNCTION: Possesses phosphatase activity toward para-nitrophenyl phosphate (pNPP) in vitro. Possesses phosphatase activity toward several phosphotyrosine-containing peptides in vitro, with low peptide substrate specificity. KEYWORDS: Hydrolase;Magnesium;Metal-binding;Protein phosphatase;Reference proteome " O82244,"PROTEIN NAMES: Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase (Alpha-1,3-mannosyltransferase ALG3) (Asparagine-linked glycosylation protein 3) (AtALG3) (Not56-like protein) PROTEIN FAMILY: Glycosyltransferase 58 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the glycosyltransferase 58 family. FUNCTION: Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. In the lumen of the endoplasmic reticulum, adds the first dolichyl beta-D-mannosyl phosphate derived mannose in an alpha-1,3 linkage to Man(5)GlcNAc(2)-PP-dolichol to produce Man(6)GlcNAc(2)-PP-dolichol. Man(6)GlcNAc(2)-PP-dolichol is a substrate for ALG9, the following enzyme in the biosynthetic pathway. PATHWAY: Protein modification; protein glycosylation. KEYWORDS: Alternative splicing;Endoplasmic reticulum;Glycoprotein;Glycosyltransferase;Membrane;Reference proteome;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein MISCELLANEOUS: In the absence of ALG3 activity, the N-glycans transferred to proteins are aberrant, indicating that the oligosaccharyltransferase (OST) complex is substrate-tolerant." O82261,"PROTEIN NAMES: Protease Do-like 2, chloroplastic PROTEIN FAMILY: Peptidase S1C family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the peptidase S1C family. FUNCTION: Serine protease that performs the primary cleavage of the photodamaged D1 protein in plant photosystem II. KEYWORDS: 3D-structure;Alternative splicing;Chloroplast;Hydrolase;Membrane;Plastid;Protease;Reference proteome;Serine protease;Thylakoid;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane ; Peripheral membrane protein ; Stromal side " O82266,"PROTEIN NAMES: Protein SLOW WALKER 1 (Protein EMBRYO SAC DEVELOPMENT ARREST 13) (Protein EMBRYO SAC DEVELOPMENT ARREST 19) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Essential protein required for nuclear division and organization during embryo sac development in female gametophyte, probably by promoting rRNA biogenesis essential for the progression of the mitotic division cycles during gametogenesis. Involved in nucleolar processing of pre-18S ribosomal RNA. KEYWORDS: Cell cycle;Cell division;Developmental protein;Nucleus;Reference proteome;Repeat;rRNA processing;WD repeat SUBCELLULAR LOCATION: Nucleus, nucleolus Note=Localized in the nucleolus in interphase cells. " O82302,PROTEIN NAMES: Protein phosphatase 2C 29 (AtPP2C29) (Protein POLTERGEIST-LIKE 1) (Protein phosphatase 2C PLL1) (PP2C PLL1) PROTEIN FAMILY: PP2C family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the PP2C family. FUNCTION: Involved in the regulation of pedicel length and of CLAVATA pathways controlling stem cell identity at shoot and flower meristems. KEYWORDS: Developmental protein;Hydrolase;Magnesium;Manganese;Metal-binding;Nucleus;Phosphoprotein;Protein phosphatase;Reference proteome SUBCELLULAR LOCATION: Nucleus O82381,"PROTEIN NAMES: Flavonol 3-O-glucosyltransferase UGT71C1 (Flavonol 7-O-beta-glucosyltransferase UGT71C1) (UDP-glycosyltransferase 71C1) PROTEIN FAMILY: UDP-glycosyltransferase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the UDP-glycosyltransferase family. FUNCTION: Possesses quercetin 7-O-glucosyltransferase and 3'-O-glucosyltransferase activities in vitro. Also active in vitro on benzoates and benzoate derivatives. Glucosylates other secondary metabolites in vitro like trans-resveratrol, curcumin, vanillin and etoposide. KEYWORDS: Glycosyltransferase;Reference proteome;Transferase " O82872,"PROTEIN NAMES: D-threonine aldolase PROTEIN FAMILY: DSD1 family ORGANISM: Arthrobacter sp SIMILARITY: Belongs to the DSD1 family. FUNCTION: Catalyzes the reversible cleavage of D-threonine or D-allothreonine into glycine and acetaldehyde. Can also cleave D-beta-phenylserine, D-beta-hydroxy-alpha-aminovaleric acid, D-beta-3,4-dihydroxyphenylserine and D-beta-3,4-methylenedioxyphenylserine into glycine and the corresponding aldehyde compounds. Inactive towards D-serine, beta-hydroxyaspartate and O-phospho-DL-threonine. KEYWORDS: Direct protein sequencing;Lyase;Pyridoxal phosphate " O84947,"PROTEIN NAMES: SPI-2 type 3 secretion system translocon protein SctE (SPI-2 T3SS translocon protein SctE) (Secreted effector protein SseC) (Secretion system effector C) PROTEIN FAMILY: SctE/SipB/YopB family ORGANISM: Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) SIMILARITY: Belongs to the SctE/SipB/YopB family. FUNCTION: Component of the type III secretion system 2 (SPI-2 T3SS), also called injectisome, which is used to inject bacterial effector proteins into eukaryotic host cells (Probable). SseC/SctE2 and SseD/SctB2 are inserted into the host membrane where they form a pore and allow the translocation of effector proteins into the cytosol of target cells (Probable).; FUNCTION: Required for the translocation of SPI-2 effector proteins. Required for systemic Salmonella infection of the mouse. Essential for SpvB-induced actin depolymerization in the host cell cytoplasm. KEYWORDS: Coiled coil;Host membrane;Membrane;Reference proteome;Secreted;Transmembrane;Transmembrane helix;Virulence SUBCELLULAR LOCATION: Secreted Cell surface Host membrane ; Multi-pass membrane protein Note=Secreted via the type III secretion system 2 (SPI-2 T3SS). After secretion, localizes mainly on the surface of the bacterial cell. " O85673,"PROTEIN NAMES: Anthranilate 1,2-dioxygenase large subunit PROTEIN FAMILY: Bacterial ring-hydroxylating dioxygenase alpha subunit family ORGANISM: Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1) SIMILARITY: Belongs to the bacterial ring-hydroxylating dioxygenase alpha subunit family. FUNCTION: Component of anthranilate dioxygenase multicomponent enzyme system which catalyzes the incorporation of both atoms of molecular oxygen into anthranilate to form catechol. PATHWAY: Aromatic compound metabolism; anthranilate degradation via hydroxylation; catechol from anthranilate: step 1/1. KEYWORDS: 2Fe-2S;Aromatic hydrocarbons catabolism;Dioxygenase;Iron;Iron-sulfur;Metal-binding;NAD;Oxidoreductase;Reference proteome " O88319,"PROTEIN NAMES: Neurotensin receptor type 1 (NT-R-1) (NTR1) PROTEIN FAMILY: G-protein coupled receptor 1 family, Neurotensin receptor subfamily, NTSR1 sub-subfamily ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the G-protein coupled receptor 1 family. Neurotensin receptor subfamily. NTSR1 sub-subfamily. FUNCTION: G-protein coupled receptor for the tridecapeptide neurotensin (NTS). Signaling is effected via G proteins that activate a phosphatidylinositol-calcium second messenger system. Signaling leads to the activation of downstream MAP kinases and protects cells against apoptosis. KEYWORDS: Cell membrane;Disulfide bond;G-protein coupled receptor;Glycoprotein;Lipoprotein;Membrane;Palmitate;Receptor;Reference proteome;Transducer;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Membrane raft Note=Palmitoylation is required for localization at CAV1-enriched membrane rafts. " O88384,PROTEIN NAMES: Vesicle transport through interaction with t-SNAREs homolog 1B (Vesicle transport v-SNARE protein Vti1-like 1) (Vti1-rp1) PROTEIN FAMILY: VTI1 family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the VTI1 family. FUNCTION: V-SNARE that mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane. These interactions are proposed to mediate aspects of the specificity of vesicle trafficking and to promote fusion of the lipid bilayers. KEYWORDS: 3D-structure;Acetylation;Coiled coil;Endosome;Lysosome;Membrane;Methylation;Phosphoprotein;Protein transport;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Early endosome membrane ; Single-pass type IV membrane protein Late endosome membrane ; Single-pass type IV membrane protein Lysosome membrane Cytoplasmic granule Recycling endosome membrane ; Single-pass type IV membrane protein O88397,"PROTEIN NAMES: Solute carrier organic anion transporter family member 1A5 (Organic anion-transporting polypeptide 3) (OATP-3) (Sodium-independent organic anion transporter 3) (Solute carrier family 21 member 7) PROTEIN FAMILY: Organo anion transporter family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the organo anion transporter (TC 2.A.60) family. FUNCTION: Na(+)-independent transporter that mediates the cellular uptake of a broad range of organic anions such as the endogenous bile salts cholate and deoxycholate, either in their unconjugated or conjugated forms (taurocholate and glycocholate), estrone 3-sulfate and prostaglandin E2, at the plasma membrane. Responsible for intestinal absorption of bile acids. Capable of thyroid hormone transport (both T3 or 3,3',5'-triiodo-L-thyronine, and T4 or L-tyroxine). Plays roles in blood-brain and -cerebrospinal fluid barrier transport of organic anions and signal mediators, and in hormone uptake by neural cells (By similarity). May also play a role in the reuptake of neuropeptides such as substance P/TAC1 and vasoactive intestinal peptide/VIP released from retinal neurons (By similarity). Shows a pH-sensitive substrate specificity which may be ascribed to the protonation state of the binding site and leads to a stimulation of substrate transport in an acidic microenvironment. Hydrogencarbonate/HCO3(-) acts as the probable counteranion that exchanges for organic anions. May contribute to regulate the transport of organic compounds in testis across the blood-testis-barrier (By similarity). KEYWORDS: Cell membrane;Disulfide bond;Glycoprotein;Ion transport;Lipid transport;Membrane;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Basal cell membrane ; Multi-pass membrane protein " O88454,"PROTEIN NAMES: Potassium channel subfamily K member 4 (TWIK-related arachidonic acid-stimulated potassium channel protein) (TRAAK) PROTEIN FAMILY: Two pore domain potassium channel family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the two pore domain potassium channel (TC 1.A.1.8) family. FUNCTION: Voltage-insensitive potassium channel. Channel opening is triggered by mechanical forces that deform the membrane. Channel opening is triggered by raising the intracellular pH to basic levels (By similarity). The channel is inactive at 24 degrees Celsius (in vitro); raising the temperature to 37 degrees Celsius increases the frequency of channel opening, with a further increase in channel activity when the temperature is raised to 42 degrees Celsius (By similarity). Plays a role in the sensory perception of pain caused by pressure. Plays a role in the perception of pain caused by heat. KEYWORDS: 3D-structure;Alternative splicing;Cell membrane;Disulfide bond;Glycoprotein;Ion channel;Ion transport;Membrane;Potassium;Potassium channel;Potassium transport;Reference proteome;Transmembrane;Transmembrane helix;Transport;Voltage-gated channel SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein " O88483,"PROTEIN NAMES: [Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 1, mitochondrial (PDP 1) (Protein phosphatase 2C) (Pyruvate dehydrogenase phosphatase catalytic subunit 1) (PDPC 1) PROTEIN FAMILY: PP2C family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the PP2C family. FUNCTION: Mitochondrial enzyme that catalyzes the dephosphorylation and concomitant reactivation of the alpha subunit of the E1 component of the pyruvate dehydrogenase complex (PDC), thereby stimulating the conversion of pyruvate into acetyl-CoA. KEYWORDS: 3D-structure;Acetylation;Calcium;Hydrolase;Magnesium;Metal-binding;Mitochondrion;Protein phosphatase;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Mitochondrion " O88507,"PROTEIN NAMES: Ciliary neurotrophic factor receptor subunit alpha (CNTF receptor subunit alpha) (CNTFR-alpha) PROTEIN FAMILY: Type I cytokine receptor family, Type 3 subfamily ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the type I cytokine receptor family. Type 3 subfamily. FUNCTION: Binds to CNTF. The alpha subunit provides the receptor specificity (By similarity). KEYWORDS: Cell membrane;Disulfide bond;Glycoprotein;GPI-anchor;Immunoglobulin domain;Lipoprotein;Membrane;Receptor;Reference proteome;Repeat;Signal SUBCELLULAR LOCATION: Cell membrane ; Lipid-anchor, GPI-anchor " O88575,"PROTEIN NAMES: Sodium- and chloride-dependent transporter XTRP3B (IMINO-K) (Solute carrier family 6 member 20B) PROTEIN FAMILY: Sodium:neurotransmitter symporter (SNF) family, SLC6A20 subfamily ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A20 subfamily. FUNCTION: Does not show transporter activity with a range of tested amino acids including proline, glutamine, glutamic acid, leucine, alanine, histidine, glycine and arginine. KEYWORDS: Cell membrane;Glycoprotein;Membrane;Reference proteome;Symport;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Apical cell membrane ; Multi-pass membrane protein Note=Located in the apical brush border membrane of kidney proximal tubule cells. " O88618,"PROTEIN NAMES: Formimidoyltransferase-cyclodeaminase (58 kDa microtubule-binding protein) (Formiminotransferase-cyclodeaminase) (FTCD) [Includes: Glutamate formimidoyltransferase (Glutamate formiminotransferase) (Glutamate formyltransferase); Formimidoyltetrahydrofolate cyclodeaminase (Formiminotetrahydrofolate cyclodeaminase)] PROTEIN FAMILY: Cyclodeaminase/cyclohydrolase family; Formiminotransferase family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: In the C-terminal section; belongs to the cyclodeaminase/cyclohydrolase family.; SIMILARITY: In the N-terminal section; belongs to the formiminotransferase family. FUNCTION: Folate-dependent enzyme, that displays both transferase and deaminase activity. Serves to channel one-carbon units from formiminoglutamate to the folate pool.; FUNCTION: Binds and promotes bundling of vimentin filaments originating from the Golgi. PATHWAY: Amino-acid degradation; L-histidine degradation into L-glutamate; L-glutamate from N-formimidoyl-L-glutamate (transferase route): step 1/1. KEYWORDS: 3D-structure;Cytoplasm;Cytoskeleton;Direct protein sequencing;Folate-binding;Golgi apparatus;Histidine metabolism;Lyase;Multifunctional enzyme;Phosphoprotein;Reference proteome;Transferase SUBCELLULAR LOCATION: Cytoplasm, cytosol Golgi apparatus Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole Note=More abundantly located around the mother centriole. " O88621,PROTEIN NAMES: Forkhead box protein H1 (Forkhead activin signal transducer 1) (Fast-1) (Forkhead activin signal transducer 2) (Fast-2) ORGANISM: Mus musculus (Mouse) FUNCTION: Transcriptional activator. Recognizes and binds to the DNA sequence 5'-TGT[GT][GT]ATT-3'. Required for induction of the goosecoid (GSC) promoter by TGF-beta or activin signaling. Forms a transcriptionally active complex containing FOXH1/SMAD2/SMAD4 on a site on the GSC promoter called TARE (TGF-beta/activin response element). KEYWORDS: Activator;Alternative splicing;DNA-binding;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus. MISCELLANEOUS: [Isoform Short]: Fails to bind DNA and cannot confer activin response element responsiveness. O88627,PROTEIN NAMES: Sodium/nucleoside cotransporter 2 (Concentrative nucleoside transporter 2) (CNT 2) (Na(+)/nucleoside cotransporter 2) (Sodium-coupled nucleoside transporter 2) (Sodium/purine nucleoside cotransporter) (SPNT) (Solute carrier family 28 member 2) PROTEIN FAMILY: Concentrative nucleoside transporter (CNT) family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the concentrative nucleoside transporter (CNT) (TC 2.A.41) family. FUNCTION: Sodium-dependent and purine-selective transporter. Exhibits the transport characteristics of the nucleoside transport system cif or N1 subtype (N1/cif) (selective for purine nucleosides and uridine). Plays a critical role in specific uptake and salvage of purine nucleosides in kidney and other tissues. May contribute to regulate the transport of organic compounds in testes across the blood-testis-barrier (By similarity). KEYWORDS: Cell membrane;Membrane;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Membrane ; Multi-pass membrane protein Apicolateral cell membrane ; Multi-pass membrane protein O88634,PROTEIN NAMES: Proteinase-activated receptor 4 (PAR-4) (Coagulation factor II receptor-like 3) (Thrombin receptor-like 3) PROTEIN FAMILY: G-protein coupled receptor 1 family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the G-protein coupled receptor 1 family. FUNCTION: Receptor for activated thrombin or trypsin coupled to G proteins that stimulate phosphoinositide hydrolysis. May play a role in platelets activation. KEYWORDS: 3D-structure;Blood coagulation;Cell membrane;Disulfide bond;G-protein coupled receptor;Glycoprotein;Hemostasis;Membrane;Receptor;Reference proteome;Signal;Transducer;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. O88667,"PROTEIN NAMES: GTP-binding protein RAD PROTEIN FAMILY: Small GTPase superfamily, RGK family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the small GTPase superfamily. RGK family. FUNCTION: May regulate basal voltage-dependent L-type Ca(2+) currents and be required for beta-adrenergic augmentation of Ca(2+) influx in cardiomyocytes, thereby regulating increases in heart rate and contractile force. May play an important role in cardiac antiarrhythmia via the strong suppression of voltage-dependent L-type Ca(2+) currents. Regulates voltage-gated L-type calcium channel subunit alpha-1C trafficking to the cell membrane. Inhibits cardiac hypertrophy through the calmodulin-dependent kinase II (CaMKII) pathway. Inhibits phosphorylation and activation of CAMK2D (By similarity). KEYWORDS: Calmodulin-binding;Cell membrane;GTP-binding;Membrane;Methylation;Nucleotide-binding;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cell membrane " O88693,"PROTEIN NAMES: Ceramide glucosyltransferase (GLCT-1) (Glucosylceramide synthase) (GCS) (Glycosylceramide synthase) (UDP-glucose ceramide glucosyltransferase) (UDP-glucose:N-acylsphingosine D-glucosyltransferase) PROTEIN FAMILY: Glycosyltransferase 2 family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the glycosyltransferase 2 family. FUNCTION: Participates in the initial step of the glucosylceramide-based glycosphingolipid/GSL synthetic pathway at the cytosolic surface of the Golgi. Catalyzes the transfer of glucose from UDP-glucose to ceramide to produce glucosylceramide/GlcCer (such as beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine). Glucosylceramide is the core component of glycosphingolipids/GSLs, amphipathic molecules consisting of a ceramide lipid moiety embedded in the outer leaflet of the membrane, linked to one of hundreds of different externally oriented oligosaccharide structures. Glycosphingolipids are essential components of membrane microdomains that mediate membrane trafficking and signal transduction. They are implicated in many fundamental cellular processes, including growth, differentiation, migration, morphogenesis, cell-to-cell and cell-to-matrix interactions. They are required for instance in the proper development and functioning of the nervous system. As an example of their role in signal transduction, they regulate the leptin receptor/LEPR in the leptin-mediated signaling pathway. They also play an important role in the establishment of the skin barrier regulating keratinocyte differentiation and the proper assembly of the cornified envelope. The biosynthesis of GSLs is also required for the proper intestinal endocytic uptake of nutritional lipids. Catalyzes the synthesis of xylosylceramide/XylCer (such as beta-D-xylosyl-(1<->1')-N-acylsphing-4-enine) using UDP-Xyl as xylose donor. PATHWAY: Lipid metabolism; sphingolipid metabolism. KEYWORDS: Acetylation;Glycosyltransferase;Golgi apparatus;Lipid biosynthesis;Lipid metabolism;Membrane;Reference proteome;Sphingolipid metabolism;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus membrane ; Multi-pass membrane protein " O88721,"PROTEIN NAMES: Vasopressin V2 receptor (V2R) (AVPR V2) (Antidiuretic hormone receptor) (Renal-type arginine vasopressin receptor) PROTEIN FAMILY: G-protein coupled receptor 1 family, Vasopressin/oxytocin receptor subfamily ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the G-protein coupled receptor 1 family. Vasopressin/oxytocin receptor subfamily. FUNCTION: Receptor for arginine vasopressin. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Involved in renal water reabsorption (By similarity). KEYWORDS: Cell membrane;G-protein coupled receptor;Glycoprotein;Lipoprotein;Membrane;Palmitate;Receptor;Reference proteome;Transducer;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein " O88819,"PROTEIN NAMES: 4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase 9 (Fucosyltransferase 9) (Fucosyltransferase IX) (Fuc-TIX) (FucT-IX) (Galactoside 3-L-fucosyltransferase) PROTEIN FAMILY: Glycosyltransferase 10 family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the glycosyltransferase 10 family. FUNCTION: Catalyzes alpha(1->3) linkage of fucosyl moiety transferred from GDP-beta-L-fucose to N-acetyl glucosamine (GlcNAc) within type 2 lactosamine (LacNAc, beta-D-Gal-(1->4)-beta-D-GlcNAc-) glycan attached to glycolipids and N- or O-linked glycoproteins. Fucosylates distal type 2 LacNAc and its fucosylated (H-type 2 LacNAc) and sialylated (sialyl-type 2 LacNAc) derivatives to form Lewis x (Lex) (CD15) and Lewis y (Ley) antigenic epitopes involved in cell adhesion and differentiation (By similarity). Generates Lex epitopes in the brain, presumably playing a role in the maintenance of neuronal stemness and neurite outgrowth in progenitor neural cells (By similarity). Fucosylates the internal type 2 LacNAc unit of the polylactosamine chain to form VIM-2 antigen that serves as recognition epitope for SELE (By similarity). Can also modify milk oligosaccharides in particular type 2 tetrasaccharide LNnT (By similarity). PATHWAY: Protein modification; protein glycosylation.; PATHWAY: Glycolipid biosynthesis. KEYWORDS: Disulfide bond;Glycoprotein;Glycosyltransferase;Golgi apparatus;Lipid metabolism;Membrane;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus, trans-Golgi network membrane ; Single-pass type II membrane protein Golgi apparatus membrane " O88944,"PROTEIN NAMES: Potassium voltage-gated channel subfamily KQT member 3 (KQT-like 3) (Potassium channel subunit alpha KvLQT3) (Voltage-gated potassium channel subunit Kv7.3) PROTEIN FAMILY: Potassium channel family, KQT subfamily, Kv7.3/KCNQ3 sub-subfamily ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the potassium channel family. KQT (TC 1.A.1.15) subfamily. Kv7.3/KCNQ3 sub-subfamily. FUNCTION: Associates with KCNQ2 or KCNQ5 to form a potassium channel with essentially identical properties to the channel underlying the native M-current, a slowly activating and deactivating potassium conductance which plays a critical role in determining the subthreshold electrical excitability of neurons as well as the responsiveness to synaptic inputs. Therefore, it is important in the regulation of neuronal excitability. KCNQ2-KCNQ3 channel is selectively permeable to other cations besides potassium, in decreasing order of affinity K(+) > Rb(+) > Cs(+) > Na(+). Associates with Na(+)-coupled myo-inositol symporter SLC5A3 forming a coregulatory complex that alters ion selectivity, increasing Na(+) and Cs(+) permeation relative to K(+) permeation (By similarity). KEYWORDS: Alternative splicing;Cell membrane;Ion channel;Ion transport;Membrane;Phosphoprotein;Potassium;Potassium channel;Potassium transport;Reference proteome;Transmembrane;Transmembrane helix;Transport;Ubl conjugation;Voltage-gated channel SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein " O88978,"PROTEIN NAMES: Dynein axonemal assembly factor 11 (DNAAF11) (Leucine-rich repeat-containing protein 6) (Leucine-rich testis-specific protein) (Protein tilB homolog) (Testis-specific leucine-rich repeat protein) PROTEIN FAMILY: TilB family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the tilB family. FUNCTION: Involved in dynein arm assembly, is important for expression and transporting outer dynein arm (ODA) proteins from the cytoplasm to the cilia. Acts as a crucial component in the formation and motility of spermatozoal flagella (By similarity). KEYWORDS: Cell projection;Cilium;Coiled coil;Cytoplasm;Flagellum;Leucine-rich repeat;Reference proteome;Repeat SUBCELLULAR LOCATION: Cytoplasm Cell projection, cilium Dynein axonemal particle Cell projection, cilium, flagellum " O89017,"PROTEIN NAMES: Legumain (Protease, cysteine 1) PROTEIN FAMILY: Peptidase C13 family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the peptidase C13 family. FUNCTION: Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. Involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system (By similarity). Also involved in MHC class I antigen presentation in cross-presenting dendritic cells by mediating cleavage and maturation of Perforin-2 (MPEG1), thereby promoting antigen translocation in the cytosol. Required for normal lysosomal protein degradation in renal proximal tubules. Required for normal degradation of internalized EGFR. Plays a role in the regulation of cell proliferation via its role in EGFR degradation. KEYWORDS: 3D-structure;Disulfide bond;Glycoprotein;Hydrolase;Lysosome;Protease;Reference proteome;Signal;Thiol protease;Zymogen SUBCELLULAR LOCATION: Lysosome " O89039,"PROTEIN NAMES: Atypical chemokine receptor 3 (C-X-C chemokine receptor type 7) (CXC-R7) (CXCR-7) (Chemokine orphan receptor 1) (G-protein coupled receptor RDC1 homolog) (RDC-1) PROTEIN FAMILY: G-protein coupled receptor 1 family, Atypical chemokine receptor subfamily ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the G-protein coupled receptor 1 family. Atypical chemokine receptor subfamily. FUNCTION: Atypical chemokine receptor that controls chemokine levels and localization via high-affinity chemokine binding that is uncoupled from classic ligand-driven signal transduction cascades, resulting instead in chemokine sequestration, degradation, or transcytosis. Also known as interceptor (internalizing receptor) or chemokine-scavenging receptor or chemokine decoy receptor. Acts as a receptor for chemokines CXCL11 and CXCL12/SDF1. Chemokine binding does not activate G-protein-mediated signal transduction but instead induces beta-arrestin recruitment, leading to ligand internalization and activation of MAPK signaling pathway. Required for regulation of CXCR4 protein levels in migrating interneurons, thereby adapting their chemokine responsiveness. In glioma cells, transduces signals via MEK/ERK pathway, mediating resistance to apoptosis. Promotes cell growth and survival. Not involved in cell migration, adhesion or proliferation of normal hematopoietic progenitors but activated by CXCL11 in malignant hemapoietic cells, leading to phosphorylation of ERK1/2 (MAPK3/MAPK1) and enhanced cell adhesion and migration. Plays a regulatory role in CXCR4-mediated activation of cell surface integrins by CXCL12. Required for heart valve development.; FUNCTION: Atypical chemokine receptor that controls chemokine levels and localization via high-affinity chemokine binding that is uncoupled from classic ligand-driven signal transduction cascades, resulting instead in chemokine sequestration, degradation, or transcytosis. Also known as interceptor (internalizing receptor) or chemokine-scavenging receptor or chemokine decoy receptor. Acts as a receptor for chemokines CXCL11 and CXCL12/SDF1 (By similarity). Chemokine binding does not activate G-protein-mediated signal transduction but instead induces beta-arrestin recruitment, leading to ligand internalization and activation of MAPK signaling pathway. Required for regulation of CXCR4 protein levels in migrating interneurons, thereby adapting their chemokine responsiveness. In glioma cells, transduces signals via MEK/ERK pathway, mediating resistance to apoptosis. Promotes cell growth and survival. Not involved in cell migration, adhesion or proliferation of normal hematopoietic progenitors but activated by CXCL11 in malignant hemapoietic cells, leading to phosphorylation of ERK1/2 (MAPK3/MAPK1) and enhanced cell adhesion and migration. Plays a regulatory role in CXCR4-mediated activation of cell surface integrins by CXCL12. Required for heart valve development. Regulates axon guidance in the oculomotor system through the regulation of CXCL12 levels (By similarity). KEYWORDS: Cell adhesion;Cell membrane;Developmental protein;Disulfide bond;Endosome;G-protein coupled receptor;Glycoprotein;Membrane;Phosphoprotein;Receptor;Reference proteome;Transducer;Transmembrane;Transmembrane helix;Ubl conjugation SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Early endosome Recycling endosome Note=Predominantly localizes to endocytic vesicles, and upon stimulation by the ligand is internalized via clathrin-coated pits in a beta-arrestin -dependent manner. Once internalized, the ligand dissociates from the receptor, and is targeted to degradation while the receptor is recycled back to the cell membrane (By similarity). " O93257,"PROTEIN NAMES: X-ray repair cross-complementing protein 5 (5'-deoxyribose-5-phosphate lyase Ku70) (5'-dRP/AP lyase Ku70) (ATP-dependent DNA helicase 2 subunit 1) (ATP-dependent DNA helicase II 70 kDa subunit) (DNA repair protein XRCC6) (Ku autoantigen protein p70 homolog) (Ku70) PROTEIN FAMILY: Ku70 family ORGANISM: Gallus gallus (Chicken) SIMILARITY: Belongs to the ku70 family. FUNCTION: Single-stranded DNA-dependent ATP-dependent helicase that plays a key role in DNA non-homologous end joining (NHEJ) by recruiting DNA-PK to DNA. Required for double-strand break repair and V(D)J recombination. Also has a role in chromosome translocation. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. During NHEJ, the XRCC5-XRRC6 dimer performs the recognition step: it recognizes and binds to the broken ends of the DNA and protects them from further resection. Binding to DNA may be mediated by XRCC6. The XRCC5-XRRC6 dimer acts as a regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5-XRRC6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. 5'-dRP lyase activity allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5-XRRC6 dimer together with APEX1 acts as a negative regulator of transcription (By similarity). KEYWORDS: Activator;ATP-binding;Chromosome;DNA damage;DNA recombination;DNA repair;DNA-binding;Helicase;Hydrolase;Lyase;Multifunctional enzyme;Nucleotide-binding;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus Chromosome " O93852,"PROTEIN NAMES: D-arabinono-1,4-lactone oxidase (ALO) (FAD-linked oxidoreductase ALO1) (L-galactono-gamma-lactone oxidase) PROTEIN FAMILY: Oxygen-dependent FAD-linked oxidoreductase family ORGANISM: Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) SIMILARITY: Belongs to the oxygen-dependent FAD-linked oxidoreductase family. FUNCTION: D-arabinono-1,4-lactone oxidase that catalyzes the final step of biosynthesis of D-erythroascorbic acid, an important antioxidant and one of the virulence factors enhancing the pathogenicity. Is also able to oxidize L-galactono-1,4-lactone, L-xylono-1,4-lactone and L-gulono-1,4-lactone. PATHWAY: Cofactor biosynthesis; D-erythroascorbate biosynthesis; dehydro-D-arabinono-1,4-lactone from D-arabinose: step 2/2. KEYWORDS: FAD;Flavoprotein;Oxidoreductase;Reference proteome;Virulence " O93875,"PROTEIN NAMES: Delta(7)-sterol 5(6)-desaturase (C-5 sterol desaturase) (Ergosterol Delta(5,6) desaturase) (Ergosterol biosynthesis protein 3) (Sterol-C5-desaturase) PROTEIN FAMILY: Sterol desaturase family ORGANISM: Candida albicans (Yeast) SIMILARITY: Belongs to the sterol desaturase family. FUNCTION: C-5 sterol desaturase; part of the third module of ergosterol biosynthesis pathway that includes the late steps of the pathwa. ERG3 catalyzes the introduction of a C-5 double bond in the B ring to produce 5-dehydroepisterol. The third module or late pathway involves the ergosterol synthesis itself through consecutive reactions that mainly occur in the endoplasmic reticulum (ER) membrane. Firstly, the squalene synthase ERG9 catalyzes the condensation of 2 farnesyl pyrophosphate moieties to form squalene, which is the precursor of all steroids. Squalene synthase is crucial for balancing the incorporation of farnesyl diphosphate (FPP) into sterol and nonsterol isoprene synthesis. Secondly, the squalene epoxidase ERG1 catalyzes the stereospecific oxidation of squalene to (S)-2,3-epoxysqualene, which is considered to be a rate-limiting enzyme in steroid biosynthesis. Then, the lanosterol synthase ERG7 catalyzes the cyclization of (S)-2,3 oxidosqualene to lanosterol, a reaction that forms the sterol core. In the next steps, lanosterol is transformed to zymosterol through a complex process involving various demethylation, reduction and desaturation reactions. The lanosterol 14-alpha-demethylase ERG11 (also known as CYP51) catalyzes C14-demethylation of lanosterol to produce 4,4'-dimethyl cholesta-8,14,24-triene-3-beta-ol, which is critical for ergosterol biosynthesis. The C-14 reductase ERG24 reduces the C14=C15 double bond of 4,4-dimethyl-cholesta-8,14,24-trienol to produce 4,4-dimethyl-cholesta-8,24-dienol. 4,4-dimethyl-cholesta-8,24-dienol is substrate of the C-4 demethylation complex ERG25-ERG26-ERG27 in which ERG25 catalyzes the three-step monooxygenation required for the demethylation of 4,4-dimethyl and 4alpha-methylsterols, ERG26 catalyzes the oxidative decarboxylation that results in a reduction of the 3-beta-hydroxy group at the C-3 carbon to an oxo group, and ERG27 is responsible for the reduction of the keto group on the C-3. ERG28 has a role as a scaffold to help anchor ERG25, ERG26 and ERG27 to the endoplasmic reticulum and ERG29 regulates the activity of the iron-containing C4-methylsterol oxidase ERG25. Then, the sterol 24-C-methyltransferase ERG6 catalyzes the methyl transfer from S-adenosyl-methionine to the C-24 of zymosterol to form fecosterol. The C-8 sterol isomerase ERG2 catalyzes the reaction which results in unsaturation at C-7 in the B ring of sterols and thus converts fecosterol to episterol. The sterol-C5-desaturase ERG3 then catalyzes the introduction of a C-5 double bond in the B ring to produce 5-dehydroepisterol. The C-22 sterol desaturase ERG5 further converts 5-dehydroepisterol into ergosta-5,7,22,24(28)-tetraen-3beta-ol by forming the C-22(23) double bond in the sterol side chain. Finally, ergosta-5,7,22,24(28)-tetraen-3beta-ol is substrate of the C-24(28) sterol reductase ERG4 to produce ergosterol (Probable). PATHWAY: Steroid metabolism; ergosterol biosynthesis; ergosterol from zymosterol: step 3/5. KEYWORDS: Endoplasmic reticulum;Iron;Lipid biosynthesis;Lipid metabolism;Membrane;Oxidoreductase;Steroid biosynthesis;Steroid metabolism;Sterol biosynthesis;Sterol metabolism;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein MISCELLANEOUS: Defects in C-5 sterol desaturation results in antibiotic and azole resistance of Candida albicans during infection, particularly in AIDS patients." O94235,"PROTEIN NAMES: Serine/threonine-protein kinase mph1 PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. FUNCTION: Involved in the regulation of the onset of mitosis. Involved in a pathway that coordinates cell proliferation and differentiation. KEYWORDS: ATP-binding;Cell cycle;Cell division;Kinase;Mitosis;Nucleotide-binding;Reference proteome;Serine/threonine-protein kinase;Transferase " O94364,PROTEIN NAMES: Wings apart-like protein homolog 1 PROTEIN FAMILY: WAPL family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the WAPL family. FUNCTION: Regulator of sister chromatid cohesion in mitosis which negatively regulates cohesin association with chromatin. KEYWORDS: Cell cycle;Cell division;Chromosome;Mitosis;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus. Chromosome. O94396,PROTEIN NAMES: tRNA pseudouridine synthase 1 (tRNA pseudouridylate synthase 1) (tRNA-uridine isomerase 1) PROTEIN FAMILY: TRNA pseudouridine synthase TruA family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the tRNA pseudouridine synthase TruA family. FUNCTION: Formation of pseudouridine at positions 27 and 28 in the anticodon stem and loop of transfer RNAs; at positions 34 and 36 of intron-containing precursor tRNA(Ile) and at position 35 in the intron-containing tRNA(Tyr). Catalyzes pseudouridylation at position 44 in U2 snRNA (By similarity). Also catalyzes pseudouridylation of mRNAs (By similarity). KEYWORDS: Isomerase;Metal-binding;mRNA processing;Nucleus;Phosphoprotein;Reference proteome;tRNA processing;Zinc SUBCELLULAR LOCATION: Nucleus O94466,PROTEIN NAMES: Probable Rho-GTPase-activating protein 7 ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) KEYWORDS: Coiled coil;GTPase activation;Phosphoprotein;Reference proteome O94487,"PROTEIN NAMES: Serine/threonine-protein kinase ppk35 (Meiotically up-regulated gene 27 protein) PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. FUNCTION: Has a role in meiosis. KEYWORDS: ATP-binding;Cytoplasm;Kinase;Meiosis;Nucleotide-binding;Nucleus;Phosphoprotein;Reference proteome;Serine/threonine-protein kinase;Transferase SUBCELLULAR LOCATION: Cytoplasm Nucleus, nucleolus " O94505,"PROTEIN NAMES: 3'(2'),5'-bisphosphate nucleotidase (3'(2'),5-bisphosphonucleoside 3'(2')-phosphohydrolase) (DPNPase) (Halotolerance protein tol1) (Inositol-polyphosphate 1-phosphatase) (Target of lithium protein 1) PROTEIN FAMILY: Inositol monophosphatase superfamily ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the inositol monophosphatase superfamily. FUNCTION: Phosphatase that converts adenosine 3'-phosphate 5'-phosphosulfate (PAPS) to adenosine 5'-phosphosulfate (APS) and 3'(2')-phosphoadenosine 5'-phosphate (PAP) to AMP. May regulate the flux of sulfur in the sulfur-activation pathway by converting PAPS to APS. Is also able to hydrolyze inositol 1,4-bisphosphate (Ins(1,4)P2) and inositol 1,3,4-trisphosphate (Ins(1,3,4)P3), but is not active on inositol 1,4,5-trisphosphate, inositol 1-phosphate, fructose 1,6-bisphosphate, AMP and ATP.; FUNCTION: Confers resistance to lithium. KEYWORDS: Hydrolase;Magnesium;Metal-binding;Nucleotide-binding;Reference proteome " O94529,"PROTEIN NAMES: FACT complex subunit pob3 (Facilitates chromatin transcription complex subunit pob3) PROTEIN FAMILY: SSRP1 family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the SSRP1 family. FUNCTION: Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II (By similarity). KEYWORDS: Chromosome;DNA damage;DNA repair;DNA replication;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus Chromosome Note=Colocalizes with RNA polymerase II on chromatin. Recruited to actively transcribed loci. MISCELLANEOUS: In contrast to the orthologous protein in animals and plants, this protein does not contain a HMG box DNA-binding domain. This function may instead be provided by the HMG box of the associated nhp6 protein in the FACT complex of fungi." O94614,PROTEIN NAMES: Chitin synthase regulatory factor 4 (Chs four homolog 3) ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) FUNCTION: Involved in septum formation. Required for the proper localization of chs2 at the septum. KEYWORDS: Cell cycle;Cell division;Cytoplasm;Phosphoprotein;Reference proteome;Repeat;Septation SUBCELLULAR LOCATION: Cytoplasm Note=Localizes to the cell tips and septum during vegetative growth. O94640,"PROTEIN NAMES: NAD-dependent histone deacetylase sir2 (Regulatory protein sir2) (Silent information regulator 2) PROTEIN FAMILY: Sirtuin family, Class I subfamily ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the sirtuin family. Class I subfamily. FUNCTION: Involved in silencing within the mating-type region, at the telomeres, and according to previous research also within centromeric DNA regions. Required for the localization of swi6 to the telomeres, silent mating type region, and according to previous research to the centromeric DNA regions. According to previous research not required for the localization of swi6 to centromeric foci. Deacetylates histone H3 on 'Lys-9' and 'Lys-16' of histone H4. This has a direct role in heterochromatin assembly. KEYWORDS: Centromere;Chromatin regulator;Chromosome;DNA damage;DNA repair;Metal-binding;NAD;Nucleus;Phosphoprotein;Reference proteome;Repressor;Telomere;Transcription;Transcription regulation;Transferase;Zinc SUBCELLULAR LOCATION: Nucleus Chromosome, centromere Chromosome, telomere Note=Nuclear throughout the cell cycle. Binds to centromeres, telomeric sites and sites between the silent mating-type loci. " O94652,"PROTEIN NAMES: mRNA export factor gle1 (Nuclear pore protein gle1) (Nucleoporin gle1) (RNA export factor gle1) PROTEIN FAMILY: GLE1 family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the GLE1 family. FUNCTION: Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. It is specifically involved in a terminal step of poly(A)+ mRNA transport through the NPC (By similarity). KEYWORDS: Coiled coil;Cytoplasm;Membrane;mRNA transport;Nuclear pore complex;Nucleus;Protein transport;Reference proteome;Translocation;Transport SUBCELLULAR LOCATION: Cytoplasm Nucleus, nuclear pore complex Nucleus membrane ; Peripheral membrane protein ; Cytoplasmic side Nucleus membrane ; Peripheral membrane protein ; Nucleoplasmic side " O94680,"PROTEIN NAMES: Phospholipid:diacylglycerol acyltransferase (PDAT) (Pombe LRO1 homolog 1) (Triacylglycerol synthase) (TAG synthase) PROTEIN FAMILY: AB hydrolase superfamily, Lipase family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the AB hydrolase superfamily. Lipase family. FUNCTION: Catalyzes triacylglycerol (TAG) formation by an acyl-CoA independent pathway. The enzyme specifically transfers acyl groups from the sn-2 position of a phospholipid to diacylglycerol (DAG), thus forming an sn-1-lysophospholipid. Plays a major role in triacylglycerol formation at log phase. Involved in lipid particle synthesis from the endoplasmic reticulum, promoting localized TAG production at discrete ER subdomains. PATHWAY: Glycerolipid metabolism; triacylglycerol biosynthesis. KEYWORDS: Acyltransferase;Endoplasmic reticulum;Membrane;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Single-pass type II membrane protein " O94720,PROTEIN NAMES: Transcription factor fil1 (GATA zinc finger domain-containing protein C1393.08) (GCN four induction-like protein) (General control transcription factor fil1) ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) FUNCTION: Activates genes required for amino acid biosynthesis and acts as a master transcriptional regulator during amino acid starvation. Binds variations of the DNA sequence 5'-GAT[AC]GC-3'. KEYWORDS: Activator;Cytoplasm;DNA-binding;Metal-binding;Nucleus;Reference proteome;Repeat;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Cytoplasm MISCELLANEOUS: Functional homolog of the S.cerevisiae GCN4 and mammalian ATF4 bZIP transcription factors. O94740,"PROTEIN NAMES: Hsp90 co-chaperone Cdc37 (Cell division control protein 37) (Hsp90 chaperone protein kinase-targeting subunit) PROTEIN FAMILY: CDC37 family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the CDC37 family. FUNCTION: Co-chaperone that binds to numerous kinases and promotes their interaction with the Hsp90 complex, resulting in stabilization and promotion of their activity. KEYWORDS: Cell cycle;Cell division;Chaperone;Cytoplasm;Nucleus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=When in the nucleus associated with chromatin. " O94818,"PROTEIN NAMES: Nucleolar protein 4 (Nucleolar-localized protein) ORGANISM: Homo sapiens (Human) KEYWORDS: Alternative splicing;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus, nucleolus " O94888,"PROTEIN NAMES: UBX domain-containing protein 7 ORGANISM: Homo sapiens (Human) FUNCTION: Ubiquitin-binding adapter that links a subset of NEDD8-associated cullin ring ligases (CRLs) to the segregase VCP/p97, to regulate turnover of their ubiquitination substrates. KEYWORDS: 3D-structure;Acetylation;Isopeptide bond;Nucleus;Phosphoprotein;Reference proteome;Ubl conjugation SUBCELLULAR LOCATION: Nucleus " O94927,"PROTEIN NAMES: HAUS augmin-like complex subunit 5 PROTEIN FAMILY: HAUS5 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the HAUS5 family. FUNCTION: Contributes to mitotic spindle assembly, maintenance of centrosome integrity and completion of cytokinesis as part of the HAUS augmin-like complex. KEYWORDS: 3D-structure;Acetylation;Alternative splicing;Cell cycle;Cell division;Coiled coil;Cytoplasm;Cytoskeleton;Microtubule;Mitosis;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Cytoplasm, cytoskeleton, spindle Note=Localizes to interphase centrosomes and to mitotic spindle microtubules. " O94955,"PROTEIN NAMES: Rho-related BTB domain-containing protein 3 ORGANISM: Homo sapiens (Human) FUNCTION: Rab9-regulated ATPase required for endosome to Golgi transport. Involved in transport vesicle docking at the Golgi complex, possibly by participating in release M6PRBP1/TIP47 from vesicles to permit their efficient docking and fusion at the Golgi. Specifically binds Rab9, but not other Rab proteins. Has low intrinsic ATPase activity due to autoinhibition, which is relieved by Rab9. KEYWORDS: ATP-binding;Golgi apparatus;Hydrolase;Nucleotide-binding;Reference proteome;Repeat;Transport SUBCELLULAR LOCATION: Golgi apparatus " O94956,"PROTEIN NAMES: Solute carrier organic anion transporter family member 2B1 (Organic anion transporter B) (OATP-B) (Organic anion transporter polypeptide-related protein 2) (OATP-RP2) (OATPRP2) (Organic anion transporting polypeptide 2B1) (OATP2B1) (Solute carrier family 21 member 9) PROTEIN FAMILY: Organo anion transporter family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the organo anion transporter (TC 2.A.60) family. FUNCTION: Mediates the Na(+)-independent transport of steroid sulfate conjugates and other specific organic anions. Responsible for the transport of estrone 3-sulfate (E1S) through the basal membrane of syncytiotrophoblast, highlighting a potential role in the placental absorption of fetal-derived sulfated steroids including the steroid hormone precursor dehydroepiandrosterone sulfate (DHEA-S). Also facilitates the uptake of sulfated steroids at the basal/sinusoidal membrane of hepatocytes, therefore accounting for the major part of organic anions clearance of liver. Mediates the intestinal uptake of sulfated steroids. Mediates the uptake of the neurosteroids DHEA-S and pregnenolone sulfate (PregS) into the endothelial cells of the blood-brain barrier as the first step to enter the brain. Also plays a role in the reuptake of neuropeptides such as substance P/TAC1 and vasoactive intestinal peptide/VIP released from retinal neurons. May act as a heme transporter that promotes cellular iron availability via heme oxygenase/HMOX2 and independently of TFRC. Also transports heme by-product coproporphyrin III (CPIII), and may be involved in their hepatic disposition. Mediates the uptake of other substrates such as prostaglandins D2 (PGD2), E1 (PGE1) and E2 (PGE2), taurocholate, L-thyroxine, leukotriene C4 and thromboxane B2. May contribute to regulate the transport of organic compounds in testis across the blood-testis-barrier (Probable). Shows a pH-sensitive substrate specificity which may be ascribed to the protonation state of the binding site and leads to a stimulation of substrate transport in an acidic microenvironment. The exact transport mechanism has not been yet deciphered but most likely involves an anion exchange, coupling the cellular uptake of organic substrate with the efflux of an anionic compound. Hydrogencarbonate/HCO3(-) acts as a probable counteranion that exchanges for organic anions. Cytoplasmic glutamate may also act as counteranion in the placenta. An inwardly directed proton gradient has also been proposed as the driving force of E1S uptake with a (H(+):E1S) stoichiometry of (1:1).; FUNCTION: [Isoform 3]: Has estrone 3-sulfate (E1S) transport activity comparable with the full-length isoform 1. KEYWORDS: Alternative promoter usage;Alternative splicing;Cell membrane;Disulfide bond;Glycoprotein;Ion transport;Membrane;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Basal cell membrane ; Multi-pass membrane protein Basolateral cell membrane ; Multi-pass membrane protein Apical cell membrane ; Multi-pass membrane protein Note=Expressed at the basal membrane of hepatocytes, syncytiotrophoblast and Sertoli cells. Localized to the basolateral membrane of enterocytes. Also found at the apical membrane of enterocytes. MISCELLANEOUS: Most likely contributes to the oral absorption and the disposition of a wide range of drugs in the intestine and the liver." O94985,"PROTEIN NAMES: Calsyntenin-1 (Alcadein-alpha) (Alc-alpha) (Alzheimer-related cadherin-like protein) (Non-classical cadherin XB31alpha) [Cleaved into: Soluble Alc-alpha (SAlc-alpha); CTF1-alpha (C-terminal fragment 1-alpha)] PROTEIN FAMILY: Calsyntenin family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the calsyntenin family. FUNCTION: Postsynaptic adhesion molecule that binds to presynaptic neurexins to mediate both excitatory and inhibitory synapse formation (By similarity). Promotes synapse development by acting as a cell adhesion molecule at the postsynaptic membrane, which associates with neurexin-alpha at the presynaptic membrane (By similarity). Also functions as a cargo in axonal anterograde transport by acting as a molecular adapter that promotes KLC1 association with vesicles. Complex formation with APBA2 and APP, stabilizes APP metabolism and enhances APBA2-mediated suppression of beta-APP40 secretion, due to the retardation of intracellular APP maturation.; FUNCTION: [Soluble Alc-alpha]: As intracellular fragment AlcICD, suppresses APBB1-dependent transactivation stimulated by APP C-terminal intracellular fragment (AICD), most probably by competing with AICD for APBB1-binding.; FUNCTION: [CTF1-alpha]: In complex with APBA2 and C99, a C-terminal APP fragment, abolishes C99 interaction with PSEN1 and thus APP C99 cleavage by gamma-secretase, most probably through stabilization of the direct interaction between APBA2 and APP. KEYWORDS: Alternative splicing;Calcium;Cell adhesion;Cell membrane;Cell projection;Direct protein sequencing;Endoplasmic reticulum;Glycoprotein;Golgi apparatus;Membrane;Nucleus;Postsynaptic cell membrane;Reference proteome;Repeat;Signal;Synapse;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Postsynaptic cell membrane ; Single-pass type I membrane protein Endoplasmic reticulum membrane ; Single-pass type I membrane protein Golgi apparatus membrane ; Single-pass type I membrane protein Cell projection, neuron projection Note=Localized in the postsynaptic membrane of both excitatory and inhibitory synapses.; SUBCELLULAR LOCATION: [Soluble Alc-alpha]: Nucleus Note=The AlcICD fragment is translocated to the nucleus upon interaction with APBB1. " O95073,"PROTEIN NAMES: Fibrinogen silencer-binding protein ORGANISM: Homo sapiens (Human) FUNCTION: Transcriptional repressor that down-regulates the expression of the fibrinogen gamma chain. Represses transcription of GSK3B gene promoter via its interaction with APBA1. KEYWORDS: Alternative splicing;Isopeptide bond;Nucleus;Reference proteome;Repressor;Transcription;Transcription regulation;Ubl conjugation SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: Intragenic, in the second intron of RAB54B gene." O95096,"PROTEIN NAMES: Homeobox protein Nkx-2.2 (Homeobox protein NK-2 homolog B) PROTEIN FAMILY: NK-2 homeobox family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the NK-2 homeobox family. FUNCTION: Transcriptional activator involved in the development of insulin-producting beta cells in the endocrine pancreas (By similarity). May also be involved in specifying diencephalic neuromeric boundaries, and in controlling the expression of genes that play a role in axonal guidance. Binds to elements within the NEUROD1 promoter (By similarity). KEYWORDS: Activator;Developmental protein;DNA-binding;Homeobox;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " O95125,"PROTEIN NAMES: Zinc finger protein 202 (Zinc finger protein with KRAB and SCAN domains 10) ORGANISM: Homo sapiens (Human) FUNCTION: Transcriptional repressor that binds to elements found predominantly in genes that participate in lipid metabolism. Among its targets are structural components of lipoprotein particles (apolipoproteins AIV, CIII, and E), enzymes involved in lipid processing (lipoprotein lipase, lecithin cholesteryl ester transferase), transporters involved in lipid homeostasis (ABCA1, ABCG1), and several genes involved in processes related to energy metabolism and vascular disease. KEYWORDS: Alternative splicing;DNA-binding;Isopeptide bond;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Repeat;Repressor;Transcription;Transcription regulation;Ubl conjugation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus. " O95171,PROTEIN NAMES: Sciellin ORGANISM: Homo sapiens (Human) FUNCTION: May function in the assembly or regulation of proteins in the cornified envelope. The LIM domain may be involved in homotypic or heterotypic associations and may function to localize sciellin to the cornified envelope. KEYWORDS: Acetylation;Alternative splicing;Cytoplasm;Direct protein sequencing;LIM domain;Membrane;Metal-binding;Phosphoprotein;Reference proteome;Repeat;Zinc SUBCELLULAR LOCATION: Cytoplasm. Membrane. Note=May become cross-linked to membrane proteins by transglutaminase. O95201,"PROTEIN NAMES: Transcriptional repressor RHIT (Repressor of heat-inducible transcription) (RhitH) (Zinc finger protein 205) (Zinc finger protein 210) PROTEIN FAMILY: Krueppel C2H2-type zinc-finger protein family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein family. FUNCTION: Transcriptional repressor involved in regulating MPV17L expression. By regulating MPV17L expression, contributes to the regulation of genes involved in H(2)O(2) metabolism and the mitochondrial apoptotic cascade. KEYWORDS: DNA-binding;Isopeptide bond;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Repeat;Transcription;Transcription regulation;Ubl conjugation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus " O95210,"PROTEIN NAMES: Starch-binding domain-containing protein 1 (Genethonin-1) (Glycophagy cargo receptor STBD1) ORGANISM: Homo sapiens (Human) FUNCTION: Acts as a cargo receptor for glycogen. Delivers its cargo to an autophagic pathway called glycophagy, resulting in the transport of glycogen to lysosomes. KEYWORDS: Autophagy;Carbohydrate metabolism;Cell membrane;Endoplasmic reticulum;Glycogen metabolism;Membrane;Phosphoprotein;Reference proteome;Signal-anchor;Transmembrane;Transmembrane helix;Ubl conjugation SUBCELLULAR LOCATION: Preautophagosomal structure membrane ; Single-pass type III membrane protein Endoplasmic reticulum membrane ; Single-pass type III membrane protein Cell membrane, sarcolemma, T-tubule Note=Also detected near the junctional sarcoplasmic reticulum. Concentrates at perinuclear structures. " O95236,PROTEIN NAMES: Apolipoprotein L3 (Apolipoprotein L-III) (ApoL-III) (TNF-inducible protein CG12-1) (CG12_1) PROTEIN FAMILY: Apolipoprotein L family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the apolipoprotein L family. FUNCTION: May affect the movement of lipids in the cytoplasm or allow the binding of lipids to organelles. KEYWORDS: Alternative splicing;Cytoplasm;Lipid transport;Reference proteome;Transport SUBCELLULAR LOCATION: Cytoplasm O95238,PROTEIN NAMES: SAM pointed domain-containing Ets transcription factor (Prostate epithelium-specific Ets transcription factor) (Prostate-specific Ets) (Prostate-derived Ets factor) PROTEIN FAMILY: ETS family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the ETS family. FUNCTION: May function as an androgen-independent transactivator of the prostate-specific antigen (PSA) promoter. Binds to 5'-GGAT-3' DNA sequences. May play a role in the regulation of the prostate gland and/or prostate cancer development. Acts as a transcriptional activator for SERPINB5 promoter. KEYWORDS: 3D-structure;Activator;Alternative splicing;DNA-binding;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus O95243,PROTEIN NAMES: Methyl-CpG-binding domain protein 4 (Methyl-CpG-binding endonuclease 1) (Methyl-CpG-binding protein MBD4) (Mismatch-specific DNA N-glycosylase) ORGANISM: Homo sapiens (Human) FUNCTION: Mismatch-specific DNA N-glycosylase involved in DNA repair. Has thymine glycosylase activity and is specific for G:T mismatches within methylated and unmethylated CpG sites. Can also remove uracil or 5-fluorouracil in G:U mismatches. Has no lyase activity. Was first identified as methyl-CpG-binding protein. KEYWORDS: 3D-structure;Alternative splicing;Disease variant;DNA damage;DNA repair;DNA-binding;Hydrolase;Nucleus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Nucleus. MISCELLANEOUS: [Isoform 4]: Possesses uracil DNA glycosylase but not thymine DNA glycosylase activity. O95263,"PROTEIN NAMES: High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B (HsPDE8B) (Cell proliferation-inducing gene 22 protein) PROTEIN FAMILY: Cyclic nucleotide phosphodiesterase family, PDE8 subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the cyclic nucleotide phosphodiesterase family. PDE8 subfamily. FUNCTION: Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in specific signaling in the thyroid gland. PATHWAY: Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1. KEYWORDS: Alternative splicing;cAMP;Cushing syndrome;Disease variant;Hydrolase;Metal-binding;Phosphoprotein;Reference proteome MISCELLANEOUS: [Isoform 1]: Major isoform." O95264,"PROTEIN NAMES: 5-hydroxytryptamine receptor 3B (5-HT3-B) (5-HT3B) (Serotonin receptor 3B) PROTEIN FAMILY: Ligand-gated ion channel family, 5-hydroxytryptamine receptor subfamily, HTR3B sub-subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the ligand-gated ion channel (TC 1.A.9) family. 5-hydroxytryptamine receptor (TC 1.A.9.2) subfamily. HTR3B sub-subfamily. FUNCTION: Forms serotonin (5-hydroxytryptamine/5-HT3)-activated cation-selective channel complexes, which when activated cause fast, depolarizing responses in neurons. KEYWORDS: Alternative splicing;Cell membrane;Disulfide bond;Glycoprotein;Ion channel;Ion transport;Ligand-gated ion channel;Membrane;Postsynaptic cell membrane;Receptor;Reference proteome;Signal;Synapse;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Postsynaptic cell membrane ; Multi-pass membrane protein Cell membrane ; Multi-pass membrane protein Note=Presumably retained within the endoplasmic reticulum unless complexed with HTR3A. " O95279,"PROTEIN NAMES: Potassium channel subfamily K member 5 (Acid-sensitive potassium channel protein TASK-2) (TWIK-related acid-sensitive K(+) channel 2) PROTEIN FAMILY: Two pore domain potassium channel family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the two pore domain potassium channel (TC 1.A.1.8) family. FUNCTION: pH-dependent, outwardly rectifying potassium channel. Outward rectification is lost at high external K(+) concentrations. KEYWORDS: Disulfide bond;Glycoprotein;Ion channel;Ion transport;Membrane;Phosphoprotein;Potassium;Potassium channel;Potassium transport;Reference proteome;Transmembrane;Transmembrane helix;Transport;Voltage-gated channel SUBCELLULAR LOCATION: Membrane ; Multi-pass membrane protein MISCELLANEOUS: Inhibited by quinine, quinidine and external acidification." O95361,"PROTEIN NAMES: Tripartite motif-containing protein 16 (E3 ubiquitin-protein ligase TRIM16) (Estrogen-responsive B box protein) PROTEIN FAMILY: TRIM/RBCC family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the TRIM/RBCC family. FUNCTION: E3 ubiquitin ligase that plays an essential role in the organization of autophagic response and ubiquitination upon lysosomal and phagosomal damages. Plays a role in the stress-induced biogenesis and degradation of protein aggresomes by regulating the p62-KEAP1-NRF2 signaling and particularly by modulating the ubiquitination levels and thus stability of NRF2. Acts as a scaffold protein and facilitates autophagic degradation of protein aggregates by interacting with p62/SQSTM, ATG16L1 and LC3B/MAP1LC3B. In turn, protects the cell against oxidative stress-induced cell death as a consequence of endomembrane damage. KEYWORDS: 3D-structure;Alternative splicing;Coiled coil;Cytoplasm;Metal-binding;Phosphoprotein;Reference proteome;Repeat;Transferase;Ubl conjugation;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cytoplasm " O95365,"PROTEIN NAMES: Zinc finger and BTB domain-containing protein 7A (Factor binding IST protein 1) (FBI-1) (Factor that binds to inducer of short transcripts protein 1) (HIV-1 1st-binding protein 1) (Leukemia/lymphoma-related factor) (POZ and Krueppel erythroid myeloid ontogenic factor) (POK erythroid myeloid ontogenic factor) (Pokemon) (Pokemon 1) (TTF-I-interacting peptide 21) (TIP21) (Zinc finger protein 857A) ORGANISM: Homo sapiens (Human) FUNCTION: Transcription factor that represses the transcription of a wide range of genes involved in cell proliferation and differentiation. Directly and specifically binds to the consensus sequence 5'-[GA][CA]GACCCCCCCCC-3' and represses transcription both by regulating the organization of chromatin and through the direct recruitment of transcription factors to gene regulatory regions. Negatively regulates SMAD4 transcriptional activity in the TGF-beta signaling pathway through these two mechanisms. That is, recruits the chromatin regulator HDAC1 to the SMAD4-DNA complex and in parallel prevents the recruitment of the transcriptional activators CREBBP and EP300. Collaborates with transcription factors like RELA to modify the accessibility of gene transcription regulatory regions to secondary transcription factors (By similarity). Also directly interacts with transcription factors like SP1 to prevent their binding to DNA. Functions as an androgen receptor/AR transcriptional corepressor by recruiting NCOR1 and NCOR2 to the androgen response elements/ARE on target genes. Thereby, negatively regulates androgen receptor signaling and androgen-induced cell proliferation. Involved in the switch between fetal and adult globin expression during erythroid cells maturation. Through its interaction with the NuRD complex regulates chromatin at the fetal globin genes to repress their transcription. Specifically represses the transcription of the tumor suppressor ARF isoform from the CDKN2A gene (By similarity). Efficiently abrogates E2F1-dependent CDKN2A transactivation (By similarity). Regulates chondrogenesis through the transcriptional repression of specific genes via a mechanism that also requires histone deacetylation (By similarity). Regulates cell proliferation through the transcriptional regulation of genes involved in glycolysis. Involved in adipogenesis through the regulation of genes involved in adipocyte differentiation. Plays a key role in the differentiation of lymphoid progenitors into B and T lineages (By similarity). Promotes differentiation towards the B lineage by inhibiting the T-cell instructive Notch signaling pathway through the specific transcriptional repression of Notch downstream target genes (By similarity). Also regulates osteoclast differentiation (By similarity). May also play a role, independently of its transcriptional activity, in double-strand break repair via classical non-homologous end joining/cNHEJ (By similarity). Recruited to double-strand break sites on damage DNA, interacts with the DNA-dependent protein kinase complex and directly regulates its stability and activity in DNA repair (By similarity). May also modulate the splicing activity of KHDRBS1 toward BCL2L1 in a mechanism which is histone deacetylase-dependent and thereby negatively regulates the pro-apoptotic effect of KHDRBS1. KEYWORDS: 3D-structure;Developmental protein;Differentiation;Disease variant;DNA-binding;Intellectual disability;Isopeptide bond;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Repeat;Repressor;Transcription;Transcription regulation;Ubl conjugation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Note=Recruited to double-strand break sites of damaged DNA. " O95390,"PROTEIN NAMES: Growth/differentiation factor 11 (GDF-11) (Bone morphogenetic protein 11) (BMP-11) PROTEIN FAMILY: TGF-beta family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the TGF-beta family. FUNCTION: Secreted signal that acts globally to regulate anterior/posterior axial patterning during development. May play critical roles in patterning both mesodermal and neural tissues (By similarity). It is required for proper vertebral patterning and orofacial development. Signals through activin receptors type-2, ACVR2A and ACVR2B, and activin receptors type-1, ACVR1B, ACVR1C and TGFBR1 leading to the phosphorylation of SMAD2 and SMAD3. KEYWORDS: 3D-structure;Cleavage on pair of basic residues;Cytokine;Disease variant;Disulfide bond;Glycoprotein;Growth factor;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted " O95402,"PROTEIN NAMES: Mediator of RNA polymerase II transcription subunit 26 (Activator-recruited cofactor 70 kDa component) (ARC70) (Cofactor required for Sp1 transcriptional activation subunit 7) (CRSP complex subunit 7) (Mediator complex subunit 26) (Transcriptional coactivator CRSP70) PROTEIN FAMILY: Mediator complex subunit 26 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the Mediator complex subunit 26 family. FUNCTION: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors. KEYWORDS: 3D-structure;Activator;Alternative splicing;Direct protein sequencing;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " O95409,PROTEIN NAMES: Zinc finger protein ZIC 2 (Zinc finger protein of the cerebellum 2) PROTEIN FAMILY: GLI C2H2-type zinc-finger protein family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the GLI C2H2-type zinc-finger protein family. FUNCTION: Acts as a transcriptional activator or repressor. Plays important roles in the early stage of organogenesis of the CNS. Activates the transcription of the serotonin transporter SERT in uncrossed ipsilateral retinal ganglion cells (iRGCs) to refine eye-specific projections in primary visual targets. Its transcriptional activity is repressed by MDFIC. Involved in the formation of the ipsilateral retinal projection at the optic chiasm midline. Drives the expression of EPHB1 on ipsilaterally projecting growth cones. Binds to the minimal GLI-consensus sequence 5'-TGGGTGGTC-3'. Associates to the basal SERT promoter region from ventrotemporal retinal segments of retinal embryos. KEYWORDS: Activator;Cytoplasm;Developmental protein;Differentiation;Disease variant;DNA-binding;Holoprosencephaly;Isopeptide bond;Metal-binding;Neurogenesis;Nucleus;Phosphoprotein;Reference proteome;Repeat;Repressor;Transcription;Transcription regulation;Ubl conjugation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus. Cytoplasm Note=Localizes in the cytoplasm in presence of MDFIC overexpression. Both phosphorylated and unphosphorylated forms are localized in the nucleus (By similarity). O95415,"PROTEIN NAMES: Membrane protein BRI3 (Brain protein I3) (pRGR2) PROTEIN FAMILY: BRI3 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the BRI3 family. FUNCTION: Participates in tumor necrosis factor-alpha (TNF)-induced cell death. May be a target of Wnt/beta-catenin signaling in the liver. KEYWORDS: Alternative splicing;Cytoplasm;Lysosome;Membrane;Nucleus;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Lysosome membrane ; Multi-pass membrane protein.; SUBCELLULAR LOCATION: [Isoform 1]: Cytoplasm, perinuclear region Note=Co-localizes with MGAT1 and IFITM3 at the perinuclear region.; SUBCELLULAR LOCATION: [Isoform 2]: Cytoplasm Nucleus Note=Diffuse localization in the cytoplasm and nucleus. " O95500,"PROTEIN NAMES: Claudin-14 PROTEIN FAMILY: Claudin family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the claudin family. FUNCTION: Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity. KEYWORDS: Cell junction;Cell membrane;Deafness;Disease variant;Membrane;Non-syndromic deafness;Reference proteome;Tight junction;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell junction, tight junction. Cell membrane; Multi-pass membrane protein. " O95619,"PROTEIN NAMES: YEATS domain-containing protein 4 (Glioma-amplified sequence 41) (Gas41) (NuMA-binding protein 1) (NuBI-1) (NuBI1) ORGANISM: Homo sapiens (Human) FUNCTION: Chromatin reader component of the NuA4 histone acetyltransferase (HAT) complex, a complex involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. Specifically recognizes and binds acylated histone H3, with a preference for histone H3 diacetylated at 'Lys-18' and 'Lys-27' (H3K18ac and H3K27ac) or histone H3 diacetylated at 'Lys-14' and 'Lys-27' (H3K14ac and H3K27ac). Also able to recognize and bind crotonylated histone H3. May also recognize and bind histone H3 succinylated at 'Lys-122' (H3K122succ); additional evidences are however required to confirm this result in vivo. Plays a key role in histone variant H2AZ1/H2A.Z deposition into specific chromatin regions: recognizes and binds H3K14ac and H3K27ac on the promoters of actively transcribed genes and recruits NuA4-related complex to deposit H2AZ1/H2A.Z. H2AZ1/H2A.Z deposition is required for maintenance of embryonic stem cell (By similarity). KEYWORDS: 3D-structure;Chromatin regulator;Coiled coil;Direct protein sequencing;Growth regulation;Isopeptide bond;Nucleus;Reference proteome;Transcription;Transcription regulation;Ubl conjugation SUBCELLULAR LOCATION: Nucleus " O95677,"PROTEIN NAMES: Eyes absent homolog 4 PROTEIN FAMILY: HAD-like hydrolase superfamily, EYA family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the HAD-like hydrolase superfamily. EYA family. FUNCTION: Tyrosine phosphatase that specifically dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph). 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Promotes efficient DNA repair by dephosphorylating H2AX, promoting the recruitment of DNA repair complexes containing MDC1. Its function as histone phosphatase probably explains its role in transcription regulation during organogenesis. May be involved in development of the eye (By similarity). KEYWORDS: Acetylation;Activator;Alternative splicing;Cardiomyopathy;Chromatin regulator;Cytoplasm;Deafness;Developmental protein;Disease variant;DNA damage;DNA repair;Hydrolase;Isopeptide bond;Magnesium;Metal-binding;Non-syndromic deafness;Nucleus;Phosphoprotein;Protein phosphatase;Reference proteome;Transcription;Transcription regulation;Ubl conjugation SUBCELLULAR LOCATION: Cytoplasm Nucleus MISCELLANEOUS: [Isoform 3]: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay." O95760,"PROTEIN NAMES: Interleukin-33 (IL-33) (Interleukin-1 family member 11) (IL-1F11) (Nuclear factor from high endothelial venules) (NF-HEV) [Cleaved into: Interleukin-33 (95-270); Interleukin-33 (99-270); Interleukin-33 (109-270)] PROTEIN FAMILY: IL-1 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the IL-1 family. Highly divergent. FUNCTION: Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells. Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as an enhancer of polarization of alternatively activated macrophages. Acts as a chemoattractant for Th2 cells, and may function as an 'alarmin', that amplifies immune responses during tissue injury. Induces rapid UCP2-dependent mitochondrial rewiring that attenuates the generation of reactive oxygen species and preserves the integrity of Krebs cycle required for persistent production of itaconate and subsequent GATA3-dependent differentiation of inflammation-resolving alternatively activated macrophages (By similarity).; FUNCTION: In quiescent endothelia the uncleaved form is constitutively and abundantly expressed, and acts as a chromatin-associated nuclear factor with transcriptional repressor properties, it may sequester nuclear NF-kappaB/RELA, lowering expression of its targets. This form is rapidely lost upon angiogenic or pro-inflammatory activation. KEYWORDS: 3D-structure;Alternative splicing;Chromosome;Cytokine;Cytoplasm;Cytoplasmic vesicle;Direct protein sequencing;Nucleus;Reference proteome;Secreted;Transcription SUBCELLULAR LOCATION: Nucleus Chromosome Cytoplasm Cytoplasmic vesicle, secretory vesicle Secreted Note=Secreted and released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore following cleavage by CELA1. Associates with heterochromatin and mitotic chromosomes. The secretion is dependent on protein unfolding and facilitated by the cargo receptor TMED10; it results in protein translocation from the cytoplasm into the ERGIC (endoplasmic reticulum-Golgi intermediate compartment) followed by vesicle entry and secretion. MISCELLANEOUS: [Isoform 3]: Constitutively active." O95859,"PROTEIN NAMES: Tetraspanin-12 (Tspan-12) (Tetraspan NET-2) (Transmembrane 4 superfamily member 12) PROTEIN FAMILY: Tetraspanin (TM4SF) family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the tetraspanin (TM4SF) family. FUNCTION: Regulator of cell surface receptor signal transduction. Plays a central role in retinal vascularization by regulating norrin (NDP) signal transduction. Acts in concert with norrin (NDP) to promote FZD4 multimerization and subsequent activation of FZD4, leading to promote accumulation of beta-catenin (CTNNB1) and stimulate LEF/TCF-mediated transcriptional programs. Suprisingly, it only activates the norrin (NDP)-dependent activation of FZD4, while it does not activate the Wnt-dependent activation of FZD4, suggesting the existence of a Wnt-independent signaling that also promote accumulation the beta-catenin (CTNNB1) (By similarity). Acts as a regulator of membrane proteinases such as ADAM10 and MMP14/MT1-MMP. Activates ADAM10-dependent cleavage activity of amyloid precursor protein (APP). Activates MMP14/MT1-MMP-dependent cleavage activity. KEYWORDS: Alternative splicing;Angiogenesis;Cell membrane;Disease variant;Lipoprotein;Membrane;Palmitate;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein " O95900,"PROTEIN NAMES: Pseudouridylate synthase TRUB2, mitochondrial (TruB pseudouridine synthase homolog 2) (tRNA pseudouridine 55 synthase TRUB2) (Psi55 synthase TRUB2) PROTEIN FAMILY: Pseudouridine synthase TruB family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the pseudouridine synthase TruB family. FUNCTION: Minor enzyme contributing to the isomerization of uridine to pseudouridine (pseudouridylation) of specific mitochondrial mRNAs (mt-mRNAs) such as COXI and COXIII mt-mRNAs. As a component of a functional protein-RNA module, consisting of RCC1L, NGRN, RPUSD3, RPUSD4, TRUB2, FASTKD2 and 16S mitochondrial ribosomal RNA (16S mt-rRNA), controls 16S mt-rRNA abundance and is required for intra-mitochondrial translation. Also catalyzes pseudouridylation of some tRNAs, including synthesis of pseudouridine(55) from uracil-55, in the psi GC loop of a subset of tRNAs. KEYWORDS: Alternative splicing;Isomerase;Mitochondrion;mRNA processing;Reference proteome;Transit peptide;tRNA processing SUBCELLULAR LOCATION: Mitochondrion matrix Note=Localizes to mitochondrial RNA granules, platforms for post-transcriptional RNA modification and ribosome assembly. " O95965,PROTEIN NAMES: Integrin beta-like protein 1 (Osteoblast-specific cysteine-rich protein) (Ten integrin EGF-like repeat domain-containing protein) ORGANISM: Homo sapiens (Human) KEYWORDS: Alternative splicing;EGF-like domain;Glycoprotein;Reference proteome;Repeat;Secreted;Signal SUBCELLULAR LOCATION: Secreted O96006,"PROTEIN NAMES: E3 SUMO-protein ligase ZBED1 (DNA replication-related element-binding factor) (Putative Ac-like transposable element) (Zinc finger BED domain-containing protein 1) (dREF homolog) ORGANISM: Homo sapiens (Human) FUNCTION: Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase which sumoylates CHD3/Mi2-alpha, causing its release from DNA. This results in suppression of CHD3/Mi2-alpha transcription repression, increased recruitment of RNA polymerase II to gene promoters and positive regulation of transcription including H1-5 and ribosomal proteins such as: RPS6, RPL10A, and RPL12. The resulting increased transcriptional activity drives cell proliferation. Binds to 5'-TGTCG[CT]GA[CT]A-3' consensus sequences in gene promoters of ribosomal proteins.; FUNCTION: (Microbial infection) Binds to human adenovirus gene promoters and contributes to transcriptional repression and virus growth inhibition during early stages of infection. PATHWAY: Protein modification; protein sumoylation. KEYWORDS: 3D-structure;DNA-binding;Host-virus interaction;Metal-binding;Nucleus;Reference proteome;Transcription;Transcription regulation;Transferase;Ubl conjugation;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus, PML body Nucleus Note=In granular structures.; SUBCELLULAR LOCATION: Nucleus, PML body. Note=(Microbial infection) Upon interaction with human adenovirus early E1A protein, the protein is redistributed to the peripheral areas of PML bodies. MISCELLANEOUS: The gene coding for this protein is located in the pseudoautosomal region 1 (PAR1) of X and Y chromosomes." O96009,PROTEIN NAMES: Napsin-A (Aspartyl protease 4) (ASP4) (Asp 4) (Napsin-1) (TA01/TA02) PROTEIN FAMILY: Peptidase A1 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the peptidase A1 family. FUNCTION: May be involved in processing of pneumocyte surfactant precursors. KEYWORDS: Aspartyl protease;Direct protein sequencing;Disulfide bond;Glycoprotein;Hydrolase;Protease;Reference proteome;Secreted;Signal;Zymogen SUBCELLULAR LOCATION: Secreted O96011,PROTEIN NAMES: Peroxisomal membrane protein 11B (Peroxin-11B) (Peroxisomal biogenesis factor 11B) (Protein PEX11 homolog beta) (PEX11-beta) PROTEIN FAMILY: Peroxin-11 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the peroxin-11 family. FUNCTION: Involved in peroxisomal proliferation. May regulate peroxisome division by recruiting the dynamin-related GTPase DNM1L to the peroxisomal membrane. Promotes membrane protrusion and elongation on the peroxisomal surface. KEYWORDS: Acetylation;Alternative splicing;Membrane;Peroxisome;Peroxisome biogenesis;Peroxisome biogenesis disorder;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Peroxisome membrane ; Single-pass membrane protein O96019,"PROTEIN NAMES: Actin-like protein 6A (53 kDa BRG1-associated factor A) (Actin-related protein Baf53a) (ArpNbeta) (BRG1-associated factor 53A) (BAF53A) (INO80 complex subunit K) PROTEIN FAMILY: Actin family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the actin family. FUNCTION: Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Required for maximal ATPase activity of SMARCA4/BRG1/BAF190A and for association of the SMARCA4/BRG1/BAF190A containing remodeling complex BAF with chromatin/nuclear matrix. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and is required for the proliferation of neural progenitors. During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity). Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Putative core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair. KEYWORDS: 3D-structure;Acetylation;Activator;Alternative splicing;Chromatin regulator;Direct protein sequencing;Disease variant;DNA damage;DNA recombination;DNA repair;Growth regulation;Intellectual disability;Isopeptide bond;Neurogenesis;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation;Ubl conjugation SUBCELLULAR LOCATION: Nucleus " O96623,"PROTEIN NAMES: Actin-related protein 2/3 complex subunit 2 (Arp2/3 complex 34 kDa subunit) (p34-ARC) PROTEIN FAMILY: ARPC2 family ORGANISM: Dictyostelium discoideum (Social amoeba) SIMILARITY: Belongs to the ARPC2 family. FUNCTION: Functions as a component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the pointed end of the daughter actin filament. The Arp2/3 complex is involved in organizing the actin system in cell motility and chemotaxis, in phagocytosis and macropinocytosis, at late steps of endosome processing, and in mitosis. In concert with a group of other proteins, the Arp2/3 complex plays a general role in the rapid activation and adaptation of the actin system to its multiple functions. KEYWORDS: Actin-binding;Cell projection;Cytoplasm;Cytoskeleton;Reference proteome SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton. Cell projection. Cytoplasm, cytosol. Cytoplasm, cell cortex. Cell projection, pseudopodium. " O97366,"PROTEIN NAMES: Phenoloxidase-activating factor 1 (Prophenoloxidase-activating factor I) (Serine protease PPAF-1) [Cleaved into: Phenoloxidase-activating factor 1 light chain; Phenoloxidase-activating factor 1 heavy chain] PROTEIN FAMILY: Peptidase S1 family, CLIP subfamily ORGANISM: Holotrichia diomphalia (Korean black chafer) SIMILARITY: Belongs to the peptidase S1 family. CLIP subfamily. FUNCTION: Serine endopeptidase which, by cleaving prophenoloxidase PPO1 and PPO2, is required for the activation of the prophenoloxidase cascade probably following the recognition of pathogen-derived products. KEYWORDS: 3D-structure;Calcium;Direct protein sequencing;Disulfide bond;Glycoprotein;Hydrolase;Immunity;Innate immunity;Metal-binding;Protease;Secreted;Serine protease;Signal;Zymogen SUBCELLULAR LOCATION: Secreted Note=Secreted in the hemolymph. " O97554,"PROTEIN NAMES: Prostaglandin G/H synthase 1 (Cyclooxygenase-1) (COX-1) (Prostaglandin H2 synthase 1) (PGH synthase 1) (PGHS-1) (PHS 1) (Prostaglandin-endoperoxide synthase 1) PROTEIN FAMILY: Prostaglandin G/H synthase family ORGANISM: Oryctolagus cuniculus (Rabbit) SIMILARITY: Belongs to the prostaglandin G/H synthase family. FUNCTION: Dual cyclooxygenase and peroxidase that plays an important role in the biosynthesis pathway of prostanoids, a class of C20 oxylipins mainly derived from arachidonate ((5Z,8Z,11Z,14Z)-eicosatetraenoate, AA, C20:4(n-6)), with a particular role in the inflammatory response. The cyclooxygenase activity oxygenates AA to the hydroperoxy endoperoxide prostaglandin G2 (PGG2), and the peroxidase activity reduces PGG2 to the hydroxy endoperoxide prostaglandin H2 (PGH2), the precursor of all 2-series prostaglandins and thromboxanes. This complex transformation is initiated by abstraction of hydrogen at carbon 13 (with S-stereochemistry), followed by insertion of molecular O2 to form the endoperoxide bridge between carbon 9 and 11 that defines prostaglandins. The insertion of a second molecule of O2 (bis-oxygenase activity) yields a hydroperoxy group in PGG2 that is then reduced to PGH2 by two electrons. Involved in the constitutive production of prostanoids in particular in the stomach and platelets. In gastric epithelial cells, it is a key step in the generation of prostaglandins, such as prostaglandin E2 (PGE2), which plays an important role in cytoprotection. In platelets, it is involved in the generation of thromboxane A2 (TXA2), which promotes platelet activation and aggregation, vasoconstriction and proliferation of vascular smooth muscle cells. Can also use linoleate (LA, (9Z,12Z)-octadecadienoate, C18:2(n-6)) as substrate and produce hydroxyoctadecadienoates (HODEs) in a regio- and stereospecific manner, being (9R)-HODE ((9R)-hydroxy-(10E,12Z)-octadecadienoate) and (13S)-HODE ((13S)-hydroxy-(9Z,11E)-octadecadienoate) its major products. PATHWAY: Lipid metabolism; prostaglandin biosynthesis. KEYWORDS: Dioxygenase;Disulfide bond;EGF-like domain;Endoplasmic reticulum;Fatty acid biosynthesis;Fatty acid metabolism;Glycoprotein;Heme;Iron;Lipid biosynthesis;Lipid metabolism;Membrane;Metal-binding;Microsome;Oxidoreductase;Peroxidase;Prostaglandin biosynthesis;Prostaglandin metabolism;Reference proteome;Signal SUBCELLULAR LOCATION: Microsome membrane ; Peripheral membrane protein Endoplasmic reticulum membrane ; Peripheral membrane protein MISCELLANEOUS: The conversion of arachidonate to prostaglandin H2 is a 2 step reaction: a cyclooxygenase (COX) reaction which converts arachidonate to prostaglandin G2 (PGG2) and a peroxidase reaction in which PGG2 is reduced to prostaglandin H2 (PGH2). The cyclooxygenase reaction occurs in a hydrophobic channel in the core of the enzyme. The peroxidase reaction occurs at a heme-containing active site located near the protein surface. The nonsteroidal anti-inflammatory drugs (NSAIDs) binding site corresponds to the cyclooxygenase active site.; MISCELLANEOUS: Conversion of arachidonate to prostaglandin H2 is mediated by 2 different isozymes: the constitutive PTGS1 and the inducible PTGS2. PTGS1 is expressed constitutively and generally produces prostanoids acutely in response to hormonal stimuli to fine-tune physiological processes requiring instantaneous, continuous regulation (e.g. hemostasis). PTGS2 is inducible and typically produces prostanoids that mediate responses to physiological stresses such as infection and inflammation.; MISCELLANEOUS: PTGS1 and PTGS2 are the targets of nonsteroidal anti-inflammatory drugs (NSAIDs) including aspirin and ibuprofen. Aspirin is able to produce an irreversible inactivation of the enzyme through a serine acetylation. Inhibition of the PGHSs with NSAIDs acutely reduces inflammation, pain, and fever, and long-term use of these drugs reduces fatal thrombotic events, as well as the development of colon cancer and Alzheimer's disease. PTGS2 is the principal isozyme responsible for production of inflammatory prostaglandins. New generation PTGSs inhibitors strive to be selective for PTGS2, to avoid side effects such as gastrointestinal complications and ulceration." O97666,"PROTEIN NAMES: Apelin receptor (Angiotensin receptor-like 1) (G-protein coupled receptor APJ) PROTEIN FAMILY: G-protein coupled receptor 1 family ORGANISM: Macaca mulatta (Rhesus macaque) SIMILARITY: Belongs to the G-protein coupled receptor 1 family. FUNCTION: Receptor for apelin receptor early endogenous ligand (APELA) and apelin (APLN) hormones coupled to G proteins that inhibit adenylate cyclase activity. Plays a key role in early development such as gastrulation, blood vessels formation and heart morphogenesis by acting as a receptor for APELA hormone. May promote angioblast migration toward the embryonic midline, i.e. the position of the future vessel formation, during vasculogenesis. Promotes sinus venosus (SV)-derived endothelial cells migration into the developing heart to promote coronary blood vessel development. Also plays a role in various processes in adults such as regulation of blood vessel formation, blood pressure, heart contractility and heart failure.; FUNCTION: (Microbial infection) Alternative coreceptor with CD4 for HIV-1 infection; may be involved in the development of AIDS dementia. KEYWORDS: Cell membrane;Developmental protein;G-protein coupled receptor;Gastrulation;Glycoprotein;Membrane;Receptor;Reference proteome;Transducer;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Note=After exposure to apelin (APLN) or apelin receptor early endogenous ligand (APELA), internalized from the cell surface into an endosomal recycling compartment, from where it is recycled to the cell membrane. " O97799,"PROTEIN NAMES: Mast/stem cell growth factor receptor Kit (SCFR) (Proto-oncogene c-Kit) (Tyrosine-protein kinase Kit) (CD antigen CD117) PROTEIN FAMILY: Protein kinase superfamily, Tyr protein kinase family, CSF-1/PDGF receptor subfamily ORGANISM: Canis lupus familiaris (Dog) (Canis familiaris) SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily. FUNCTION: Tyrosine-protein kinase that acts as a cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1 (By similarity). KEYWORDS: Alternative splicing;ATP-binding;Cell membrane;Disulfide bond;Glycoprotein;Immunoglobulin domain;Kinase;Magnesium;Membrane;Metal-binding;Nucleotide-binding;Phosphoprotein;Proto-oncogene;Receptor;Reference proteome;Repeat;Signal;Transferase;Transmembrane;Transmembrane helix;Tyrosine-protein kinase;Ubl conjugation SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. MISCELLANEOUS: Numerous proteins are phosphorylated in response to KIT signaling, but it is not evident to determine which are directly phosphorylated by KIT under in vivo conditions." O97859,"PROTEIN NAMES: Sialidase-3 (Ganglioside sialidase) (Membrane sialidase) (N-acetyl-alpha-neuraminidase 3) PROTEIN FAMILY: Glycosyl hydrolase 33 family ORGANISM: Bos taurus (Bovine) SIMILARITY: Belongs to the glycosyl hydrolase 33 family. FUNCTION: Exo-alpha-sialidase that catalyzes the hydrolytic cleavage of the terminal sialic acid (N-acetylneuraminic acid, Neu5Ac) of a glycan moiety in the catabolism of glycolipids, glycoproteins and oligosacharides. Displays high catalytic efficiency for gangliosides including alpha-(2->3)-sialylated GD1a and GM3 and alpha-(2->8)-sialylated GD3. Plays a role in the regulation of transmembrane signaling through the modulation of ganglioside content of the lipid bilayer and by direct interaction with signaling receptors, such as EGFR. Desialylates EGFR and activates downstream signaling in proliferating cells. Contributes to clathrin-mediated endocytosis by regulating sorting of endocytosed receptors to early and recycling endosomes (By similarity). KEYWORDS: Carbohydrate metabolism;Cell membrane;Direct protein sequencing;Endosome;Glycosidase;Hydrolase;Lipid degradation;Lipid metabolism;Lipoprotein;Lysosome;Membrane;Phosphoprotein;Reference proteome;Repeat SUBCELLULAR LOCATION: Cell membrane ; Peripheral membrane protein Membrane, caveola Early endosome membrane ; Peripheral membrane protein Recycling endosome membrane ; Peripheral membrane protein Lysosome membrane ; Peripheral membrane protein Note=Associates with the external leaflet of the plasma membrane (By similarity). S-acylated NEU3 likely spans the lipid bilayer with a portion of C-terminus exposed to cytosol and the catalytic region facing the extracellular space (By similarity). " O97921,"PROTEIN NAMES: Prostaglandin-H2 D-isomerase (Glutathione-independent PGD synthase) (Lipocalin-type prostaglandin-D synthase) (Prostaglandin-D2 synthase) (PGD2 synthase) (PGDS) (PGDS2) PROTEIN FAMILY: Calycin superfamily, Lipocalin family ORGANISM: Equus caballus (Horse) SIMILARITY: Belongs to the calycin superfamily. Lipocalin family. FUNCTION: Catalyzes the conversion of PGH2 to PGD2, a prostaglandin involved in smooth muscle contraction/relaxation and a potent inhibitor of platelet aggregation. Involved in a variety of CNS functions, such as sedation, NREM sleep and PGE2-induced allodynia, and may have an anti-apoptotic role in oligodendrocytes. Binds small non-substrate lipophilic molecules, including biliverdin, bilirubin, retinal, retinoic acid and thyroid hormone, and may act as a scavenger for harmful hydrophobic molecules and as a secretory retinoid and thyroid hormone transporter. Possibly involved in development and maintenance of the blood-brain, blood-retina, blood-aqueous humor and blood-testis barrier. It is likely to play important roles in both maturation and maintenance of the central nervous system and male reproductive system (By similarity). Involved in PLA2G3-dependent maturation of mast cells. PLA2G3 is secreted by immature mast cells and acts on nearby fibroblasts upstream to PTDGS to synthesize PGD2, which in turn promotes mast cell maturation and degranulation via PTGDR (By similarity). KEYWORDS: Cytoplasm;Direct protein sequencing;Disulfide bond;Endoplasmic reticulum;Fatty acid biosynthesis;Fatty acid metabolism;Glycoprotein;Golgi apparatus;Isomerase;Lipid biosynthesis;Lipid metabolism;Mast cell degranulation;Membrane;Nucleus;Prostaglandin biosynthesis;Prostaglandin metabolism;Pyrrolidone carboxylic acid;Reference proteome;Secreted;Signal;Transport SUBCELLULAR LOCATION: Rough endoplasmic reticulum Nucleus membrane Golgi apparatus Cytoplasm, perinuclear region Secreted Note=Detected on rough endoplasmic reticulum of arachnoid and menigioma cells. Localized to the nuclear envelope, Golgi apparatus, secretory vesicles and spherical cytoplasmic structures in arachnoid trabecular cells, and to circular cytoplasmic structures in meningeal macrophages and perivascular microglial cells. In oligodendrocytes, localized to the rough endoplasmic reticulum and nuclear envelope. In retinal pigment epithelial cells, localized to distinct cytoplasmic domains including the perinuclear region. Also secreted. " P00125,"PROTEIN NAMES: Cytochrome c1, heme protein, mitochondrial (Complex III subunit 4) (Complex III subunit IV) (Cytochrome b-c1 complex subunit 4) (Ubiquinol-cytochrome-c reductase complex cytochrome c1 subunit) (Cytochrome c-1) PROTEIN FAMILY: Cytochrome c family ORGANISM: Bos taurus (Bovine) SIMILARITY: Belongs to the cytochrome c family. FUNCTION: Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. The cytochrome b-c1 complex catalyzes electron transfer from ubiquinol to cytochrome c, linking this redox reaction to translocation of protons across the mitochondrial inner membrane, with protons being carried across the membrane as hydrogens on the quinol. In the process called Q cycle, 2 protons are consumed from the matrix, 4 protons are released into the intermembrane space and 2 electrons are passed to cytochrome c. Cytochrome c1 is a catalytic core subunit containing a c-type heme. It transfers electrons from the [2Fe-2S] iron-sulfur cluster of the Rieske protein to cytochrome c. KEYWORDS: 3D-structure;Direct protein sequencing;Electron transport;Heme;Iron;Membrane;Metal-binding;Mitochondrion;Mitochondrion inner membrane;Reference proteome;Respiratory chain;Transit peptide;Translocase;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Mitochondrion inner membrane ; Single-pass membrane protein " P00175,PROTEIN NAMES: L-lactate dehydrogenase (cytochrome) (Cytochrome b2) (Flavocytochrome b2) (FCB2) (L-lactate ferricytochrome c oxidoreductase) (L-LCR) PROTEIN FAMILY: Cytochrome b5 family; FMN-dependent alpha-hydroxy acid dehydrogenase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: In the N-terminal section; belongs to the cytochrome b5 family.; SIMILARITY: In the C-terminal section; belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family. FUNCTION: Catalyzes the oxidation of (S)-lactate (L-lactate) to pyruvate with subsequent transfer of electrons to cytochrome c. Is involved in the utilization of (S)-lactate as a sole source of carbon for growth. Can also use ferricyanide as an electron acceptor in vitro. KEYWORDS: 3D-structure;Direct protein sequencing;Electron transport;Flavoprotein;FMN;Heme;Iron;Metal-binding;Mitochondrion;Oxidoreductase;Reference proteome;Respiratory chain;Transit peptide;Transport SUBCELLULAR LOCATION: Mitochondrion intermembrane space MISCELLANEOUS: Present with 4590 molecules/cell in log phase SD medium. P00363,"PROTEIN NAMES: Fumarate reductase flavoprotein subunit (Quinol-fumarate reductase flavoprotein subunit) (QFR flavoprotein subunit) PROTEIN FAMILY: FAD-dependent oxidoreductase 2 family, FRD/SDH subfamily ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily. FUNCTION: Two distinct, membrane-bound, FAD-containing enzymes are responsible for the catalysis of fumarate and succinate interconversion; fumarate reductase is used during anaerobic growth, and succinate dehydrogenase is used during aerobic growth. The QFR enzyme complex binds 2 quinones in or near the membrane; 1 near the [3Fe-4S] cluster (QP is proximal to the [3Fe-4S] cluster, on the cytoplasmic side of the membrane) while QD (the distal cluster) is on the other side of the membrane. It is not clear if both of the quinol-binding sites are functionally relevant. KEYWORDS: 3D-structure;Cell inner membrane;Cell membrane;Direct protein sequencing;Electron transport;FAD;Flavoprotein;Membrane;Nucleotide-binding;Oxidoreductase;Reference proteome;Transport SUBCELLULAR LOCATION: Cell inner membrane ; Peripheral membrane protein ; Cytoplasmic side " P00586,"PROTEIN NAMES: Thiosulfate sulfurtransferase (Rhodanese) ORGANISM: Bos taurus (Bovine) FUNCTION: Together with MRPL18, acts as a mitochondrial import factor for the cytosolic 5S rRNA. Only the nascent unfolded cytoplasmic form is able to bind to the 5S rRNA (By similarity). Formation of iron-sulfur complexes and cyanide detoxification. Binds molecular oxygen and sulfur. KEYWORDS: 3D-structure;Acetylation;Direct protein sequencing;Glycoprotein;Mitochondrion;Phosphoprotein;Reference proteome;Repeat;RNA-binding;Transferase SUBCELLULAR LOCATION: Mitochondrion matrix. " P00644,PROTEIN NAMES: Thermonuclease (TNase) (Micrococcal nuclease) (Staphylococcal nuclease) [Cleaved into: Nuclease B; Nuclease A] PROTEIN FAMILY: Thermonuclease family ORGANISM: Staphylococcus aureus SIMILARITY: Belongs to the thermonuclease family. FUNCTION: Enzyme that catalyzes the hydrolysis of both DNA and RNA at the 5' position of the phosphodiester bond. KEYWORDS: 3D-structure;Calcium;Direct protein sequencing;Endonuclease;Hydrolase;Membrane;Metal-binding;Nuclease;Secreted;Signal;Zymogen SUBCELLULAR LOCATION: [Nuclease A]: Secreted.; SUBCELLULAR LOCATION: [Nuclease B]: Membrane. P00724,PROTEIN NAMES: Invertase 2 (Beta-fructofuranosidase 2) (Saccharase) PROTEIN FAMILY: Glycosyl hydrolase 32 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the glycosyl hydrolase 32 family. KEYWORDS: 3D-structure;Alternative initiation;Cytoplasm;Direct protein sequencing;Glycoprotein;Glycosidase;Hydrolase;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: [Isoform Intracellular]: Cytoplasm.; SUBCELLULAR LOCATION: [Isoform Secreted]: Secreted. MISCELLANEOUS: Present with 1780 molecules/cell in log phase SD medium.; MISCELLANEOUS: [Isoform Intracellular]: Produced by alternative initiation at Met-21 of isoform Secreted. P00738,"PROTEIN NAMES: Haptoglobin (Zonulin) [Cleaved into: Haptoglobin alpha chain; Haptoglobin beta chain] PROTEIN FAMILY: Peptidase S1 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the peptidase S1 family. FUNCTION: As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an antioxidant, has antibacterial activity, and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidly cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway.; FUNCTION: The uncleaved form of allele alpha-2 (2-2), known as zonulin, plays a role in intestinal permeability, allowing intercellular tight junction disassembly, and controlling the equilibrium between tolerance and immunity to non-self antigens. KEYWORDS: 3D-structure;Acute phase;Alternative splicing;Antibiotic;Antimicrobial;Antioxidant;Direct protein sequencing;Disease variant;Disulfide bond;Glycoprotein;Hemoglobin-binding;Immunity;Reference proteome;Repeat;Secreted;Serine protease homolog;Signal;Sushi SUBCELLULAR LOCATION: Secreted. " P00743,"PROTEIN NAMES: Coagulation factor X (Stuart factor) [Cleaved into: Factor X light chain; Factor X heavy chain; Activated factor Xa heavy chain] PROTEIN FAMILY: Peptidase S1 family ORGANISM: Bos taurus (Bovine) SIMILARITY: Belongs to the peptidase S1 family. FUNCTION: Factor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting. KEYWORDS: 3D-structure;Blood coagulation;Calcium;Cleavage on pair of basic residues;Direct protein sequencing;Disulfide bond;EGF-like domain;Gamma-carboxyglutamic acid;Glycoprotein;Hemostasis;Hydrolase;Hydroxylation;Protease;Reference proteome;Repeat;Secreted;Serine protease;Signal;Sulfation;Zymogen SUBCELLULAR LOCATION: Secreted. MISCELLANEOUS: Calcium also binds, with stronger affinity to another site, beyond the GLA domain." P00803,PROTEIN NAMES: Signal peptidase I (SPase I) (Leader peptidase I) PROTEIN FAMILY: Peptidase S26 family ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the peptidase S26 family. KEYWORDS: 3D-structure;Cell inner membrane;Cell membrane;Disulfide bond;Hydrolase;Membrane;Protease;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell inner membrane ; Multi-pass membrane protein P00904,"PROTEIN NAMES: Bifunctional protein TrpGD [Includes: Anthranilate synthase component 2 (AS) (ASII) (Anthranilate synthase, glutamine amidotransferase component); Anthranilate phosphoribosyltransferase ] PROTEIN FAMILY: Anthranilate phosphoribosyltransferase family ORGANISM: Escherichia coli (strain K12) SIMILARITY: In the C-terminal section; belongs to the anthranilate phosphoribosyltransferase family. FUNCTION: Part of a heterotetrameric complex that catalyzes the two-step biosynthesis of anthranilate, an intermediate in the biosynthesis of L-tryptophan. In the first step, the glutamine-binding beta subunit (TrpG) of anthranilate synthase (AS) provides the glutamine amidotransferase activity which generates ammonia as a substrate that, along with chorismate, is used in the second step, catalyzed by the large alpha subunit of AS (TrpE) to produce anthranilate. In the absence of TrpG, TrpE can synthesize anthranilate directly from chorismate and high concentrations of ammonia. In addition to synthesizing anthranilate, it also catalyzes the second step of the pathway, the transfer of the phosphoribosyl group of 5-phosphorylribose-1-pyrophosphate (PRPP) to anthranilate. PATHWAY: Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 1/5.; PATHWAY: Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 2/5. KEYWORDS: Allosteric enzyme;Amino-acid biosynthesis;Aromatic amino acid biosynthesis;Direct protein sequencing;Glutamine amidotransferase;Glycosyltransferase;Lyase;Multifunctional enzyme;Reference proteome;Transferase;Tryptophan biosynthesis " P00978,"PROTEIN NAMES: Protein AMBP [Cleaved into: Alpha-1-microglobulin ; Inter-alpha-trypsin inhibitor light chain (ITI-LC) (BI-14) (Bikunin) (Cumulus extracellular matrix-stabilizing factor) (ESF) (HI-30); Trypstatin] PROTEIN FAMILY: Calycin superfamily, Lipocalin family ORGANISM: Bos taurus (Bovine) SIMILARITY: In the N-terminal section; belongs to the calycin superfamily. Lipocalin family. FUNCTION: [Alpha-1-microglobulin]: Antioxidant and tissue repair protein with reductase, heme-binding and radical-scavenging activities. Removes and protects against harmful oxidants and repairs macromolecules in intravascular and extravascular spaces and in intracellular compartments. Intravascularly, plays a regulatory role in red cell homeostasis by preventing heme- and reactive oxygen species-induced cell damage. Binds and degrades free heme to protect fetal and adult red blood cells from hemolysis. Reduces extracellular methemoglobin, a Fe3+ (ferric) form of hemoglobin that cannot bind oxygen, back to the Fe2+ (ferrous) form deoxyhemoglobin, which has oxygen-carrying potential. Upon acute inflammation, inhibits oxidation of low-density lipoprotein particles by MPO and limits vascular damage. Extravascularly, protects from oxidation products formed on extracellular matrix structures and cell membranes. Catalyzes the reduction of carbonyl groups on oxidized collagen fibers and preserves cellular and extracellular matrix ultrastructures. Importantly, counteracts the oxidative damage at blood-placenta interface, preventing leakage of free fetal hemoglobin into the maternal circulation. Intracellularly, has a role in maintaining mitochondrial redox homeostasis. Bound to complex I of the respiratory chain of mitochondria, may scavenge free radicals and preserve mitochondrial ATP synthesis. Protects renal tubule epithelial cells from heme-induced oxidative damage to mitochondria. Reduces cytochrome c from Fe3+ (ferric) to the Fe2+ (ferrous) state through formation of superoxide anion radicals in the presence of ascorbate or NADH/NADPH electron donor cofactors, ascorbate being the preferred cofactor (By similarity). Has a chaperone role in facilitating the correct folding of bikunin in the endoplasmic reticulum compartment (By similarity).; FUNCTION: [Inter-alpha-trypsin inhibitor light chain]: Kunitz-type serine protease inhibitor and structural component of extracellular matrix with a role in extracellular space remodeling and cell adhesion. Among others, has antiprotease activity toward kallikrein, a protease involved in airway inflammation; inhibits GZMK/granzyme, a granule-stored serine protease involved in NK and T cell cytotoxic responses; and inhibits PLG/plasmin, a protease required for activation of matrix metalloproteinases. As part of I-alpha-I complex, provides for the heavy chains to be transferred from I-alpha-I complex to hyaluronan in the presence of TNFAIP6, in a dynamic process that releases free bikunin and remodels extracellular matrix proteoglycan structures. Free bikunin, but not its heavy chain-bound form, acts as a potent protease inhibitor in airway secretions (By similarity). Part of hyaluronan-rich extracellular matrix that surrounds oocyte during cumulus oophorus expansion, an indispensable process for proper ovulation (By similarity). Also inhibits calcium oxalate crystallization (By similarity).; FUNCTION: [Trypstatin]: Kunitz-type serine protease inhibitor. Has high catalytic efficiency for F10/blood coagulation factor Xa and may act as an anticoagulant by inhibiting prothrombin activation. Inhibits trypsin and mast cell CMA1/chymase and tryptase proteases. KEYWORDS: Cell membrane;Chromophore;Cleavage on pair of basic residues;Cytoplasm;Direct protein sequencing;Disulfide bond;Endoplasmic reticulum;Extracellular matrix;Glycoprotein;Membrane;Mitochondrion;Mitochondrion inner membrane;Nucleus;Oxidoreductase;Protease inhibitor;Proteoglycan;Reference proteome;Repeat;Secreted;Serine protease inhibitor;Signal SUBCELLULAR LOCATION: [Alpha-1-microglobulin]: Secreted Endoplasmic reticulum Cytoplasm, cytosol Cell membrane ; Peripheral membrane protein Nucleus membrane ; Peripheral membrane protein Mitochondrion inner membrane ; Peripheral membrane protein Secreted, extracellular space, extracellular matrix Note=The cellular uptake occurs via a non-endocytotic pathway and allows for localization to various membrane structures. A specific binding to plasma membrane suggests the presence of a cell receptor, yet to be identified. Directly binds collagen fibers type I.; SUBCELLULAR LOCATION: [Inter-alpha-trypsin inhibitor light chain]: Secreted " P01186,"PROTEIN NAMES: Vasopressin-neurophysin 2-copeptin (AVP-NPII) [Cleaved into: Arg-vasopressin (Arginine-vasopressin); Neurophysin 2 (Neurophysin-I); Copeptin] PROTEIN FAMILY: Vasopressin/oxytocin family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the vasopressin/oxytocin family. FUNCTION: Neurophysin 2 specifically binds vasopressin.; FUNCTION: Vasopressin has a direct antidiuretic action on the kidney, it also causes vasoconstriction of the peripheral vessels. Acts by binding to vasopressin receptors (V1bR/AVPR1B, V1aR/AVPR1A, and V2R/AVPR2) (By similarity). KEYWORDS: Amidation;Cleavage on pair of basic residues;Direct protein sequencing;Disulfide bond;Glycoprotein;Hormone;Reference proteome;Secreted;Signal;Vasoactive;Vasoconstrictor SUBCELLULAR LOCATION: Secreted. " P01591,"PROTEIN NAMES: Immunoglobulin J chain (Joining chain of multimeric IgA and IgM) ORGANISM: Homo sapiens (Human) FUNCTION: Serves to link two monomer units of either IgM or IgA. In the case of IgM, the J chain-joined dimer is a nucleating unit for the IgM pentamer, and in the case of IgA it induces dimers and/or larger polymers. It also helps to bind these immunoglobulins to secretory component. KEYWORDS: 3D-structure;Direct protein sequencing;Disulfide bond;Glycoprotein;Pyrrolidone carboxylic acid;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted " P01731,"PROTEIN NAMES: T-cell surface glycoprotein CD8 alpha chain (T-cell surface glycoprotein Lyt-2) (CD antigen CD8a) ORGANISM: Mus musculus (Mouse) FUNCTION: Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs). This mechanism enables CTLs to recognize and eliminate infected cells and tumor cells. In NK-cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells. KEYWORDS: 3D-structure;Adaptive immunity;Alternative splicing;Cell membrane;Disulfide bond;Glycoprotein;Immunity;Immunoglobulin domain;Lipoprotein;Membrane;Palmitate;Reference proteome;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein Note=Cd8a localizes to lipid rafts only when associated with its partner Cd8b. " P02141,PROTEIN NAMES: Hemoglobin subunit beta-4 (Beta-4-globin) (Hemoglobin beta-4 chain) (Hemoglobin beta-IV chain) PROTEIN FAMILY: Globin family ORGANISM: Oncorhynchus mykiss (Rainbow trout) (Salmo gairdneri) SIMILARITY: Belongs to the globin family. FUNCTION: Involved in oxygen transport from gills to the various peripheral tissues. KEYWORDS: 3D-structure;Direct protein sequencing;Heme;Iron;Metal-binding;Oxygen transport;Transport P02679,"PROTEIN NAMES: Fibrinogen gamma chain ORGANISM: Homo sapiens (Human) FUNCTION: Together with fibrinogen alpha (FGA) and fibrinogen beta (FGB), polymerizes to form an insoluble fibrin matrix. Has a major function in hemostasis as one of the primary components of blood clots. In addition, functions during the early stages of wound repair to stabilize the lesion and guide cell migration during re-epithelialization. Was originally thought to be essential for platelet aggregation, based on in vitro studies using anticoagulated blood. However, subsequent studies have shown that it is not absolutely required for thrombus formation in vivo. Enhances expression of SELP in activated platelets via an ITGB3-dependent pathway. Maternal fibrinogen is essential for successful pregnancy. Fibrin deposition is also associated with infection, where it protects against IFNG-mediated hemorrhage. May also facilitate the antibacterial immune response via both innate and T-cell mediated pathways. KEYWORDS: 3D-structure;Alternative splicing;Blood coagulation;Calcium;Coiled coil;Direct protein sequencing;Disease variant;Disulfide bond;Glycoprotein;Hemostasis;Isopeptide bond;Metal-binding;Phosphoprotein;Reference proteome;Secreted;Signal;Sulfation SUBCELLULAR LOCATION: Secreted MISCELLANEOUS: The gamma-chain carries the main binding site for the platelet receptor.; MISCELLANEOUS: [Isoform Gamma-B]: Present in about 10% of the fibrinogen molecules in plasma but absent from those in the platelets." P02748,"PROTEIN NAMES: Complement component C9 [Cleaved into: Complement component C9a; Complement component C9b] PROTEIN FAMILY: Complement C6/C7/C8/C9 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the complement C6/C7/C8/C9 family. FUNCTION: Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C9 is the pore-forming subunit of the MAC. KEYWORDS: 3D-structure;Age-related macular degeneration;Complement alternate pathway;Complement pathway;Cytolysis;Direct protein sequencing;Disease variant;Disulfide bond;EGF-like domain;Glycoprotein;Immunity;Innate immunity;Membrane;Membrane attack complex;Phosphoprotein;Reference proteome;Secreted;Signal;Target cell membrane;Target membrane;Transmembrane;Transmembrane beta strand SUBCELLULAR LOCATION: Secreted Target cell membrane ; Multi-pass membrane protein Note=Secreted as soluble monomer. Oligomerizes at target membranes, forming a pre-pore. A conformation change then leads to the formation of a 100 Angstrom diameter pore. " P02749,"PROTEIN NAMES: Beta-2-glycoprotein 1 (APC inhibitor) (Activated protein C-binding protein) (Anticardiolipin cofactor) (Apolipoprotein H) (Apo-H) (Beta-2-glycoprotein I) (B2GPI) (Beta(2)GPI) ORGANISM: Homo sapiens (Human) FUNCTION: Binds to various kinds of negatively charged substances such as heparin, phospholipids, and dextran sulfate. May prevent activation of the intrinsic blood coagulation cascade by binding to phospholipids on the surface of damaged cells. KEYWORDS: 3D-structure;Direct protein sequencing;Disulfide bond;Glycoprotein;Heparin-binding;Reference proteome;Repeat;Secreted;Signal;Sushi SUBCELLULAR LOCATION: Secreted. " P02765,"PROTEIN NAMES: Alpha-2-HS-glycoprotein (Alpha-2-Z-globulin) (Ba-alpha-2-glycoprotein) (Fetuin-A) [Cleaved into: Alpha-2-HS-glycoprotein chain A; Alpha-2-HS-glycoprotein chain B] PROTEIN FAMILY: Fetuin family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the fetuin family. FUNCTION: Promotes endocytosis, possesses opsonic properties and influences the mineral phase of bone. Shows affinity for calcium and barium ions. KEYWORDS: Direct protein sequencing;Disease variant;Disulfide bond;Glycoprotein;Hypotrichosis;Intellectual disability;Mineral balance;Phosphoprotein;Reference proteome;Repeat;Secreted;Signal SUBCELLULAR LOCATION: Secreted. " P02773,"PROTEIN NAMES: Alpha-fetoprotein (Alpha-1-fetoprotein) (Alpha-fetoglobulin) PROTEIN FAMILY: ALB/AFP/VDB family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the ALB/AFP/VDB family. FUNCTION: Binds estrogens, fatty acids and metals. KEYWORDS: Alternative splicing;Copper;Cytoplasm;Disulfide bond;Glycoprotein;Metal-binding;Phosphoprotein;Reference proteome;Repeat;Secreted;Signal;Sulfation SUBCELLULAR LOCATION: [Isoform 1]: Secreted.; SUBCELLULAR LOCATION: [Isoform 2]: Cytoplasm. " P02844,"PROTEIN NAMES: Vitellogenin-2 (Vitellogenin II) (Yolk protein 2) PROTEIN FAMILY: AB hydrolase superfamily, Lipase family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the AB hydrolase superfamily. Lipase family. FUNCTION: Vitellogenin is the major yolk protein of eggs where it is used as a food source during embryogenesis. Vitellogenins and their receptor yl/yolkless are required for maintenance of microtubule plus-end orientation towards the posterior pole of oocytes. Involved in polarized localization of germ plasm components, such as osk mRNA and vas protein, to the oocyte posterior cortex. Receptor-mediated endocytosis by yl/yolkless is crucial for actin reorganization, mediated by osk isoform A/Long, required to anchor germ plasm components to the oocyte cortex. KEYWORDS: Direct protein sequencing;Phosphoprotein;Reference proteome;Secreted;Signal;Sulfation SUBCELLULAR LOCATION: Secreted. " P02879,"PROTEIN NAMES: Ricin [Cleaved into: Ricin A chain (rRNA N-glycosidase); Ricin B chain] PROTEIN FAMILY: Ribosome-inactivating protein family, Type 2 RIP subfamily ORGANISM: Ricinus communis (Castor bean) SIMILARITY: In the N-terminal section; belongs to the ribosome-inactivating protein family. Type 2 RIP subfamily. FUNCTION: Ricin is highly toxic to animal cells, and to a lesser extent to plant cells.; FUNCTION: [Ricin A chain]: Acts as a glycosidase that removes a specific adenine residue from an exposed loop of the 28S rRNA (A4324 in mammals), leading to rRNA breakage. As this loop is involved in elongation factor binding, modified ribosomes are catalytically inactive and unable to support protein synthesis. Can inactivate a few thousand ribosomes per minute, faster than the cell can make new ones. Therefore a single molecule can kill an animal cell.; FUNCTION: [Ricin B chain]: Binds to beta-D-galactopyranoside moieties on cell surface glycoproteins and glycolipids and facilitates the entry into the cell of the A chain. Also responsible for cell agglutination (Lectin activity). KEYWORDS: 3D-structure;Direct protein sequencing;Disulfide bond;Glycoprotein;Hydrolase;Lectin;Nucleotide-binding;Plant defense;Protein synthesis inhibitor;Repeat;Signal;Toxin " P02930,"PROTEIN NAMES: Outer membrane protein TolC (Multidrug efflux pump subunit TolC) (Outer membrane factor TolC) PROTEIN FAMILY: Outer membrane factor (OMF) family ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family. FUNCTION: Outer membrane channel, which is required for the function of several efflux systems such as AcrAB-TolC, AcrEF-TolC, EmrAB-TolC and MacAB-TolC. These systems are involved in export of antibiotics and other toxic compounds from the cell. TolC is also involved in import of colicin E1 into the cells. KEYWORDS: 3D-structure;Antibiotic resistance;Cell outer membrane;Direct protein sequencing;Membrane;Reference proteome;Repeat;Signal;Transmembrane;Transmembrane beta strand;Transport SUBCELLULAR LOCATION: Cell outer membrane ; Multi-pass membrane protein " P02976,"PROTEIN NAMES: Immunoglobulin G-binding protein A (IgG-binding protein A) (Staphylococcal protein A) (SpA) PROTEIN FAMILY: Immunoglobulin-binding protein SpA family ORGANISM: Staphylococcus aureus (strain NCTC 8325 / PS 47) SIMILARITY: Belongs to the immunoglobulin-binding protein SpA family. FUNCTION: Plays a role in the inhibition of the host innate and adaptive immune responses. Possesses five immunoglobulin-binding domains that capture both the fragment crystallizable region (Fc region) and the Fab region (part of Ig that identifies antigen) of immunoglobulins. In turn, Staphylococcus aureus is protected from phagocytic killing via inhibition of Ig Fc region. In addition, the host elicited B-cell response is prevented due to a decrease of antibody-secreting cell proliferation that enter the bone marrow, thereby decreasing long-term antibody production. Inhibits osteogenesis by preventing osteoblast proliferation and expression of alkaline phosphatase, type I collagen, osteopontin and osteocalcin. Acts directly as a pro-inflammatory factor in the lung through its ability to bind and activate tumor necrosis factor alpha receptor 1/TNFRSF1A (By similarity). KEYWORDS: 3D-structure;Cell wall;Direct protein sequencing;IgG-binding protein;Peptidoglycan-anchor;Reference proteome;Repeat;Secreted;Signal;Virulence SUBCELLULAR LOCATION: Secreted, cell wall ; Peptidoglycan-anchor ; Extracellular side Secreted Note=Cell wall anchoring is conferred by the LPXTG motif and following sequences. Anchored by sortase A. SpA from strains A676 and V-I is secreted whereas SpA from Cowan 1 and 8325-4 is mostly attached to the cell wall (Probable). Newly synthesized protein is deposited at 2-4 foci/cell and eventually is distributed in a ring around the cell. " P03023,"PROTEIN NAMES: Lactose operon repressor ORGANISM: Escherichia coli (strain K12) FUNCTION: Repressor of the lactose operon. Binds allolactose as an inducer. KEYWORDS: 3D-structure;Direct protein sequencing;DNA-binding;Reference proteome;Repressor;Transcription;Transcription regulation MISCELLANEOUS: Removing residues 1-59 results in loss of DNA-binding activity but retains tetrameric structure and inducer-binding activity. Deleting residues 340-360 results in loss of tetramer formation, but retains dimer formation, inducer-binding activity, and DNA-binding activity (if residues 1-59 are present)." P03870,"PROTEIN NAMES: Site-specific recombinase Flp (FLP) (Protein Able) PROTEIN FAMILY: 'phage' integrase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the 'phage' integrase family. FUNCTION: Part of the plasmid amplification system, which corrects any decrease in copy number caused by a rare missegregation event. Catalyzes the recombination between the large inverted repetitions of the 2-micron plasmid during plasmid replication. This recombination event changes the direction of one of the two replication forks in the bidirectionally replicating molecule, effectively resulting in multiple rounds of replication from a single initiation event. Binds specifically to the FLP recognition target (FRT) site where it induces DNA to bend. Three types of bend exist. Type I is approximately 60 degrees and results from 1 FLP molecule binding to 1 symmetry element. Type II is >144 degrees and results from FLP molecules binding to symmetry elements a and b. Type III is approximately 65 degrees and results from FLP molecules binding to symmetry elements b and c. KEYWORDS: 3D-structure;Direct protein sequencing;DNA integration;DNA recombination;DNA-binding;Plasmid;Reference proteome MISCELLANEOUS: The plasmid 2-micron circle is a extrachromosomal element that resides in the nucleus and propagates itself stably in host cell populations. It provides no obvious advantage to the host but imposes no significant disadvantage either at its steady-state copy number of 40-60 molecules/cell." P03873,"PROTEIN NAMES: Cytochrome b mRNA maturase bI2 PROTEIN FAMILY: Cytochrome b family; LAGLIDADG endonuclease family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: In the N-terminal section; belongs to the cytochrome b family.; SIMILARITY: In the C-terminal section; belongs to the LAGLIDADG endonuclease family. FUNCTION: This protein is responsible for splicing and maturation of cytochrome b mRNA. Specifically, it may be responsible for the splicing specificity of the second intron. KEYWORDS: Membrane;Mitochondrion;Mitochondrion inner membrane;mRNA processing;mRNA splicing;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Mitochondrion inner membrane ; Multi-pass membrane protein MISCELLANEOUS: Encoded from partially processed COB mRNA that terminates with the in-frame coding sequence of the second intron." P03877,"PROTEIN NAMES: Intron-encoded DNA endonuclease aI3 (DNA endonuclease I-SceIII) [Cleaved into: Truncated non-functional cytochrome oxidase 1; DNA endonuclease aI3 (Intron-encoded endonuclease I-SceIII)] PROTEIN FAMILY: LAGLIDADG endonuclease family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: In the C-terminal section; belongs to the LAGLIDADG endonuclease family. FUNCTION: Mitochondrial DNA endonuclease involved in intron homing. It introduces a specific double-strand break in the DNA of the COX1 gene and thus mediates the insertion of an intron, containing its own coding sequence (group I intron), into an intronless gene. Recognizes with high specificity and cleaves the sequence 5'-GGTTTTGGTAACTATTTATTAC-3'. KEYWORDS: Endonuclease;Hydrolase;Intron homing;Membrane;Mitochondrion;mRNA processing;mRNA splicing;Nuclease;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Mitochondrion. Membrane ; Multi-pass membrane protein MISCELLANEOUS: Strain Capensis / YB4237 has two stop codons in position 276 and 407, which disrupt the gene coding for this protein. Consequently, the corresponding intron containing its coding sequence is not mobile." P03954,"PROTEIN NAMES: Pepsin A-1 (Pepsin III-3) PROTEIN FAMILY: Peptidase A1 family ORGANISM: Macaca fuscata fuscata (Japanese macaque) SIMILARITY: Belongs to the peptidase A1 family. FUNCTION: Shows particularly broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent. KEYWORDS: Aspartyl protease;Digestion;Direct protein sequencing;Disulfide bond;Hydrolase;Phosphoprotein;Protease;Secreted;Signal;Zymogen SUBCELLULAR LOCATION: Secreted. MISCELLANEOUS: The expression of pepsinogen genes is regulated by hormones and related substances." P04004,"PROTEIN NAMES: Vitronectin (VN) (S-protein) (Serum-spreading factor) (V75) [Cleaved into: Vitronectin V65 subunit; Vitronectin V10 subunit; Somatomedin-B] ORGANISM: Homo sapiens (Human) FUNCTION: Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.; FUNCTION: Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity. KEYWORDS: 3D-structure;Cell adhesion;Direct protein sequencing;Disulfide bond;Glycoprotein;Heparin-binding;Phosphoprotein;Reference proteome;Repeat;Secreted;Signal;Sulfation SUBCELLULAR LOCATION: Secreted, extracellular space.; SUBCELLULAR LOCATION: Parasitophorous vacuole Note=(Microbial infection) In P.falciparum-infected red blood cells, VTN internalization is detected at the early trophozoite stage. Colocalizes with SERA5 at the schizont stage and with SERA5 P47 at the merozoite surface. " P04070,"PROTEIN NAMES: Vitamin K-dependent protein C (Anticoagulant protein C) (Autoprothrombin IIA) (Blood coagulation factor XIV) [Cleaved into: Vitamin K-dependent protein C light chain; Vitamin K-dependent protein C heavy chain; Activation peptide] PROTEIN FAMILY: Peptidase S1 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the peptidase S1 family. FUNCTION: Protein C is a vitamin K-dependent serine protease that regulates blood coagulation by inactivating factors Va and VIIIa in the presence of calcium ions and phospholipids. Exerts a protective effect on the endothelial cell barrier function. KEYWORDS: 3D-structure;Alternative splicing;Blood coagulation;Calcium;Cleavage on pair of basic residues;Direct protein sequencing;Disease variant;Disulfide bond;EGF-like domain;Endoplasmic reticulum;Gamma-carboxyglutamic acid;Glycoprotein;Golgi apparatus;Hemostasis;Hydrolase;Hydroxylation;Phosphoprotein;Protease;Reference proteome;Repeat;Secreted;Serine protease;Signal;Thrombophilia;Zymogen SUBCELLULAR LOCATION: Secreted Golgi apparatus Endoplasmic reticulum MISCELLANEOUS: Calcium also binds, with stronger affinity to another site, beyond the GLA domain. This GLA-independent binding site is necessary for the recognition of the thrombin-thrombomodulin complex." P04180,"PROTEIN NAMES: Phosphatidylcholine-sterol acyltransferase (1-alkyl-2-acetylglycerophosphocholine esterase) (Lecithin-cholesterol acyltransferase) (Phospholipid-cholesterol acyltransferase) (Platelet-activating factor acetylhydrolase) (PAF acetylhydrolase) PROTEIN FAMILY: AB hydrolase superfamily, Lipase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the AB hydrolase superfamily. Lipase family. FUNCTION: Central enzyme in the extracellular metabolism of plasma lipoproteins. Synthesized mainly in the liver and secreted into plasma where it converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lysophosphatidylcholines on the surface of high and low density lipoproteins (HDLs and LDLs). The cholesterol ester is then transported back to the liver. Has a preference for plasma 16:0-18:2 or 18:O-18:2 phosphatidylcholines. Also produced in the brain by primary astrocytes, and esterifies free cholesterol on nascent APOE-containing lipoproteins secreted from glia and influences cerebral spinal fluid (CSF) APOE- and APOA1 levels. Together with APOE and the cholesterol transporter ABCA1, plays a key role in the maturation of glial-derived, nascent lipoproteins. Required for remodeling high-density lipoprotein particles into their spherical forms. Catalyzes the hydrolysis of 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine (platelet-activating factor or PAF) to 1-O-alkyl-sn-glycero-3-phosphocholine (lyso-PAF). Also catalyzes the transfer of the acetate group from PAF to 1-hexadecanoyl-sn-glycero-3-phosphocholine forming lyso-PAF. Catalyzes the esterification of (24S)-hydroxycholesterol (24(S)OH-C), also known as cerebrosterol to produce 24(S)OH-C monoesters. KEYWORDS: 3D-structure;Acyltransferase;Cholesterol metabolism;Corneal dystrophy;Direct protein sequencing;Disease variant;Disulfide bond;Glycoprotein;Hydrolase;Lipid metabolism;Reference proteome;Secreted;Signal;Steroid metabolism;Sterol metabolism;Transferase SUBCELLULAR LOCATION: Secreted Note=Secreted into blood plasma. Produced in astrocytes and secreted into cerebral spinal fluid (CSF). MISCELLANEOUS: Levels of LCAT activity correlates inversely with leptin levels as well as with obesity for a wide range of BMI values." P04190,"PROTEIN NAMES: Metallo-beta-lactamase type 2 (B2 metallo-beta-lactamase) (Beta-lactamase II) (Cephalosporinase) (Metallo-beta-lactamase type II) (Metallothioprotein beta-lactamase II) (Penicillinase) (Zinc-requiring beta-lactamase II) PROTEIN FAMILY: Metallo-beta-lactamase superfamily, Class-B beta-lactamase family ORGANISM: Bacillus cereus SIMILARITY: Belongs to the metallo-beta-lactamase superfamily. Class-B beta-lactamase family. FUNCTION: Confers resistance to the different beta-lactams antibiotics (penicillin, cephalosporin and carbapenem) via the hydrolysis of the beta-lactam ring. Active on cephalosporin and penicillin. KEYWORDS: 3D-structure;Antibiotic resistance;Direct protein sequencing;Hydrolase;Metal-binding;Periplasm;Signal;Zinc SUBCELLULAR LOCATION: Periplasm " P04201,"PROTEIN NAMES: Proto-oncogene Mas PROTEIN FAMILY: G-protein coupled receptor 1 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the G-protein coupled receptor 1 family. FUNCTION: Receptor for angiotensin 1-7 (By similarity). Acts specifically as a functional antagonist of AGTR1 (angiotensin-2 type 1 receptor), although it up-regulates AGTR1 receptor levels. Positive regulation of AGTR1 levels occurs through activation of the G-proteins GNA11 and GNAQ, and stimulation of the protein kinase C signaling cascade. The antagonist effect on AGTR1 function is probably due to AGTR1 being physically altered by MAS1. KEYWORDS: Cell membrane;G-protein coupled receptor;Glycoprotein;Membrane;Proto-oncogene;Receptor;Reference proteome;Transducer;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein " P04233,"PROTEIN NAMES: HLA class II histocompatibility antigen gamma chain (HLA-DR antigens-associated invariant chain) (Ia antigen-associated invariant chain) (Ii) (CD antigen CD74) [Cleaved into: Class-II-associated invariant chain peptide (CLIP)] ORGANISM: Homo sapiens (Human) FUNCTION: Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF.; FUNCTION: [Class-II-associated invariant chain peptide]: Binds to the peptide-binding site of MHC class II alpha/beta heterodimers forming an alpha-beta-CLIP complex, thereby preventing the loading of antigenic peptides to the MHC class II complex until its release by HLA-DM in the endosome.; FUNCTION: [Isoform p41]: Stabilizes the conformation of mature CTSL by binding to its active site and serving as a chaperone to help maintain a pool of mature enzyme in endocytic compartments and extracellular space of antigen-presenting cells (APCs). Has antiviral activity by stymieing the endosomal entry of Ebola virus and coronaviruses, including SARS-CoV-2. Disrupts cathepsin-mediated Ebola virus glycoprotein processing, which prevents viral fusion and entry. This antiviral activity is specific to p41 isoform. KEYWORDS: 3D-structure;Adaptive immunity;Alternative initiation;Alternative splicing;Cell membrane;Chaperone;Direct protein sequencing;Disulfide bond;Endoplasmic reticulum;Endosome;Glycoprotein;Golgi apparatus;Immunity;Lysosome;Membrane;Phosphoprotein;Proteoglycan;Reference proteome;Secreted;Signal-anchor;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type II membrane protein Endoplasmic reticulum membrane. Golgi apparatus, trans-Golgi network. Endosome. Lysosome. Secreted Note=Transits through a number of intracellular compartments in the endocytic pathway. It can either undergo proteolysis or reach the cell membrane.; SUBCELLULAR LOCATION: [Isoform p41]: Late endosome Lysosome " P04276,"PROTEIN NAMES: Vitamin D-binding protein (DBP) (VDB) (Gc-globulin) (Group-specific component) PROTEIN FAMILY: ALB/AFP/VDB family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the ALB/AFP/VDB family. FUNCTION: Involved in vitamin D transport and storage, scavenging of extracellular G-actin, enhancement of the chemotactic activity of C5 alpha for neutrophils in inflammation and macrophage activation. KEYWORDS: Actin-binding;Direct protein sequencing;Disulfide bond;Glycoprotein;Phosphoprotein;Reference proteome;Repeat;Secreted;Signal;Transport;Vitamin D SUBCELLULAR LOCATION: Secreted " P04386,"PROTEIN NAMES: Regulatory protein GAL4 ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: This protein is a positive regulator for the gene expression of the galactose-induced genes such as GAL1, GAL2, GAL7, GAL10, and MEL1 which code for the enzymes used to convert galactose to glucose. It recognizes a 17 base pair sequence in (5'-CGGRNNRCYNYNCNCCG-3') the upstream activating sequence (UAS-G) of these genes. KEYWORDS: 3D-structure;Activator;Carbohydrate metabolism;DNA-binding;Galactose metabolism;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation;Zinc SUBCELLULAR LOCATION: Nucleus. MISCELLANEOUS: Present with 166 molecules/cell in log phase SD medium." P04635,"PROTEIN NAMES: Lipase (Phospholipase A1) (Triacylglycerol lipase) [Cleaved into: Lipase 86 kDa form; Lipase 46 kDa form] PROTEIN FAMILY: AB hydrolase superfamily, Lipase family ORGANISM: Staphylococcus hyicus SIMILARITY: Belongs to the AB hydrolase superfamily. Lipase family. FUNCTION: Has a broad substrate specificity hydrolyzing a variety of triglycerides and phosphatidylcholines. KEYWORDS: 3D-structure;Calcium;Direct protein sequencing;Hydrolase;Lipid degradation;Lipid metabolism;Metal-binding;Secreted;Signal SUBCELLULAR LOCATION: Secreted " P04694,"PROTEIN NAMES: Tyrosine aminotransferase (TAT) (L-tyrosine:2-oxoglutarate aminotransferase) PROTEIN FAMILY: Class-I pyridoxal-phosphate-dependent aminotransferase family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. FUNCTION: Transaminase involved in tyrosine breakdown. Converts tyrosine to p-hydroxyphenylpyruvate. Can catalyze the reverse reaction, using glutamic acid, with 2-oxoglutarate as cosubstrate (in vitro). Has much lower affinity and transaminase activity towards phenylalanine. PATHWAY: Amino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 2/6. KEYWORDS: Acetylation;Aminotransferase;Direct protein sequencing;Phenylalanine catabolism;Phosphoprotein;Pyridoxal phosphate;Reference proteome;Transferase;Tyrosine catabolism " P04758,"PROTEIN NAMES: Acetylcholine receptor subunit beta PROTEIN FAMILY: Ligand-gated ion channel family, Acetylcholine receptor subfamily, Beta-1/CHRNB1 sub-subfamily ORGANISM: Bos taurus (Bovine) SIMILARITY: Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Beta-1/CHRNB1 sub-subfamily. FUNCTION: After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. KEYWORDS: Cell membrane;Disulfide bond;Glycoprotein;Ion channel;Ion transport;Ligand-gated ion channel;Membrane;Phosphoprotein;Postsynaptic cell membrane;Receptor;Reference proteome;Signal;Synapse;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. " P04769,PROTEIN NAMES: Prolactin-7D1 (Proliferin-related protein) (PRP) PROTEIN FAMILY: Somatotropin/prolactin family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the somatotropin/prolactin family. KEYWORDS: Disulfide bond;Hormone;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted. P04924,"PROTEIN NAMES: Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor, membrane form (N-terminal fragment) (NTF); Intracellular domain 1 (ICD1); Intracellular domain 2 (ICD2); C-domain 1; C-domain 2; Tumor necrosis factor, soluble form] PROTEIN FAMILY: Tumor necrosis factor family ORGANISM: Oryctolagus cuniculus (Rabbit) SIMILARITY: Belongs to the tumor necrosis factor family. FUNCTION: Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation (By similarity). Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance (By similarity). Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6 (By similarity). Promotes osteoclastogenesis and therefore mediates bone resorption (By similarity).; FUNCTION: The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells. KEYWORDS: Cell membrane;Cytokine;Disulfide bond;Lipoprotein;Membrane;Myristate;Phosphoprotein;Reference proteome;Secreted;Signal-anchor;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type II membrane protein.; SUBCELLULAR LOCATION: [Tumor necrosis factor, membrane form]: Membrane ; Single-pass type II membrane protein.; SUBCELLULAR LOCATION: [Tumor necrosis factor, soluble form]: Secreted.; SUBCELLULAR LOCATION: [C-domain 1]: Secreted.; SUBCELLULAR LOCATION: [C-domain 2]: Secreted " P04963,"PROTEIN NAMES: Chloroperoxidase (Chloride peroxidase) (CPO) PROTEIN FAMILY: Chloroperoxidase family ORGANISM: Leptoxyphium fumago (Caldariomyces fumago) SIMILARITY: Belongs to the chloroperoxidase family. FUNCTION: Catalyzes peroxidative halogenations involved in the biosynthesis of clardariomycin (2,2-dichloro-1,3-cyclo-pentenedione). The enzyme also has potent catalase activity and in the absence of halide ion, acts as a peroxidase similar to plant peroxidases. KEYWORDS: 3D-structure;Chloride;Cleavage on pair of basic residues;Direct protein sequencing;Disulfide bond;Glycoprotein;Heme;Iron;Manganese;Metal-binding;Oxidoreductase;Peroxidase;Pyrrolidone carboxylic acid;Signal " P04977,"PROTEIN NAMES: Pertussis toxin subunit 1 (PTX S1) (Islet-activating protein S1) (IAP S1) (NAD-dependent ADP-ribosyltransferase) PROTEIN FAMILY: Bacterial exotoxin subunit A family ORGANISM: Bordetella pertussis (strain Tohama I / ATCC BAA-589 / NCTC 13251) SIMILARITY: Belongs to the bacterial exotoxin subunit A family. FUNCTION: S1 is an NAD-dependent ADP-ribosyltransferase, which plays a crucial role in the pathogenesis of B.pertussis causing disruption of normal host cellular regulation. It catalyzes the ADP-ribosylation of a cysteine in the alpha subunit of host heterotrimeric G proteins. In the absence of G proteins it also catalyzes the cleavage of NAD(+) into ADP-ribose and nicotinamide. It irreversibly uncouples the G-alpha GTP-binding proteins from their membrane receptors. KEYWORDS: 3D-structure;Direct protein sequencing;Disulfide bond;Glycosyltransferase;NAD;Nucleotidyltransferase;Pharmaceutical;Reference proteome;Secreted;Signal;Toxin;Transferase;Virulence;Whooping cough SUBCELLULAR LOCATION: Secreted Note=The individual chains are secreted by a sec-dependent mechanism into the periplasm. Then, S1 associates with the outer membrane before it joins with the B subunit to form the secretion-competent holotoxin. The type IV secretion system ptl mediates secretion of assembled toxin through the outer membrane. MISCELLANEOUS: Thiol:disulfide oxidoreductases DsbA and DsbB are required for periplasmic toxin assembly, whereas DbsC is important for extracellular toxin secretion.; MISCELLANEOUS: Experiments with temporal expression of PTX indicate that holotoxin secretion is at a rate of 3 molecules/min/cell. Also, more of toxin chains S1, S2 and S3 are produced than secreted; one half of each chain is incorporated into holotoxin." P04995,PROTEIN NAMES: Exodeoxyribonuclease I (ExoI) (Exonuclease I) (DNA deoxyribophosphodiesterase) (dRPase) ORGANISM: Escherichia coli (strain K12) FUNCTION: Degrades single-stranded DNA (ssDNA) in a highly processive manner. Also functions as a DNA deoxyribophosphodiesterase that releases deoxyribose-phosphate moieties following the cleavage of DNA at an apurinic/apyrimidinic (AP) site by either an AP endonuclease or AP lyase. KEYWORDS: 3D-structure;Direct protein sequencing;DNA damage;DNA repair;DNA-binding;Exonuclease;Hydrolase;Magnesium;Metal-binding;Nuclease;Reference proteome;Zinc P05060,"PROTEIN NAMES: Secretogranin-1 (Chromogranin-B) (CgB) (Secretogranin I) (SgI) [Cleaved into: PE-11; GAWK peptide; CCB peptide] PROTEIN FAMILY: Chromogranin/secretogranin protein family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the chromogranin/secretogranin protein family. FUNCTION: Secretogranin-1 is a neuroendocrine secretory granule protein, which may be the precursor for other biologically active peptides. KEYWORDS: Cleavage on pair of basic residues;Direct protein sequencing;Disulfide bond;Glycoprotein;Phosphoprotein;Proteoglycan;Reference proteome;Secreted;Signal;Sulfation SUBCELLULAR LOCATION: Secreted Note=Neuroendocrine and endocrine secretory granules. " P05066,"PROTEIN NAMES: Deoxyribodipyrimidine photo-lyase, mitochondrial (DNA photolyase) (Photoreactivating enzyme) PROTEIN FAMILY: DNA photolyase class-1 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the DNA photolyase class-1 family. FUNCTION: Involved in repair of UV radiation-induced DNA damage. Catalyzes the light-dependent monomerization (300-600 nm) of cyclobutyl pyrimidine dimers (in cis-syn configuration), which are formed between adjacent bases on the same DNA strand upon exposure to ultraviolet radiation. KEYWORDS: Chromophore;DNA damage;DNA repair;DNA-binding;FAD;Flavoprotein;Lyase;Mitochondrion;Nucleotide-binding;Nucleus;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Nucleus. Mitochondrion. MISCELLANEOUS: There are only 150-300 molecules of photolyase per yeast cell.; MISCELLANEOUS: Present with 688 molecules/cell in log phase SD medium." P05121,"PROTEIN NAMES: Plasminogen activator inhibitor 1 (PAI) (PAI-1) (Endothelial plasminogen activator inhibitor) (Serpin E1) PROTEIN FAMILY: Serpin family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the serpin family. FUNCTION: Serine protease inhibitor. Inhibits TMPRSS7. Is a primary inhibitor of tissue-type plasminogen activator (PLAT) and urokinase-type plasminogen activator (PLAU). As PLAT inhibitor, it is required for fibrinolysis down-regulation and is responsible for the controlled degradation of blood clots. As PLAU inhibitor, it is involved in the regulation of cell adhesion and spreading. Acts as a regulator of cell migration, independently of its role as protease inhibitor. It is required for stimulation of keratinocyte migration during cutaneous injury repair. It is involved in cellular and replicative senescence. Plays a role in alveolar type 2 cells senescence in the lung (By similarity). Is involved in the regulation of cementogenic differentiation of periodontal ligament stem cells, and regulates odontoblast differentiation and dentin formation during odontogenesis. KEYWORDS: 3D-structure;Alternative splicing;Direct protein sequencing;Glycoprotein;Protease inhibitor;Reference proteome;Secreted;Serine protease inhibitor;Signal SUBCELLULAR LOCATION: Secreted " P05154,"PROTEIN NAMES: Plasma serine protease inhibitor (Acrosomal serine protease inhibitor) (Plasminogen activator inhibitor 3) (PAI-3) (PAI3) (Protein C inhibitor) (PCI) (Serpin A5) PROTEIN FAMILY: Serpin family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the serpin family. FUNCTION: Heparin-dependent serine protease inhibitor acting in body fluids and secretions. Inactivates serine proteases by binding irreversibly to their serine activation site. Involved in the regulation of intravascular and extravascular proteolytic activities. Plays hemostatic roles in the blood plasma. Acts as a procoagulant and pro-inflammatory factor by inhibiting the anticoagulant activated protein C factor as well as the generation of activated protein C factor by the thrombin/thrombomodulin complex. Acts as an anticoagulant factor by inhibiting blood coagulation factors like prothrombin, factor XI, factor Xa, plasma kallikrein and fibrinolytic enzymes such as tissue- and urinary-type plasminogen activators. In seminal plasma, inactivates several serine proteases implicated in the reproductive system. Inhibits the serpin acrosin; indirectly protects component of the male genital tract from being degraded by excessive released acrosin. Inhibits tissue- and urinary-type plasminogen activator, prostate-specific antigen and kallikrein activities; has a control on the sperm motility and fertilization. Inhibits the activated protein C-catalyzed degradation of SEMG1 and SEMG2; regulates the degradation of semenogelin during the process of transfer of spermatozoa from the male reproductive tract into the female tract. In urine, inhibits urinary-type plasminogen activator and kallikrein activities. Inactivates membrane-anchored serine proteases activities such as MPRSS7 and TMPRSS11E. Inhibits urinary-type plasminogen activator-dependent tumor cell invasion and metastasis. May also play a non-inhibitory role in seminal plasma and urine as a hydrophobic hormone carrier by its binding to retinoic acid. KEYWORDS: 3D-structure;Direct protein sequencing;Fertilization;Glycoprotein;Heparin-binding;Lipid transport;Protease inhibitor;Reference proteome;Secreted;Serine protease inhibitor;Signal;Transport SUBCELLULAR LOCATION: Secreted, extracellular space Note=Localized on the plasma membrane overlying the acrosomal head of spermatozoa of ependymal spermatozoa and ejaculated sperm. Localized at the equatorial segment of acrosome-reacted spermatozoa. Localized in alpha granules in resting platelets and on the external plasma membrane and within the surface-connected cannalicular system in activated platelets. " P05221,"PROTEIN NAMES: Nucleoplasmin PROTEIN FAMILY: Nucleoplasmin family ORGANISM: Xenopus laevis (African clawed frog) SIMILARITY: Belongs to the nucleoplasmin family. FUNCTION: Acts as a chaperone for histones, such as histone H2A-H2B, and thus regulates the assembly of nucleosome cores. Involved in chromatin remodeling, especially during fertilization and early embryonic development (By similarity). May be involved in sperm chromatin decondensation during fertilization. KEYWORDS: 3D-structure;Acetylation;Chaperone;Chromatin regulator;Developmental protein;Fertilization;Methylation;Nucleus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Nucleus. " P05231,"PROTEIN NAMES: Interleukin-6 (IL-6) (B-cell stimulatory factor 2) (BSF-2) (CTL differentiation factor) (CDF) (Hybridoma growth factor) (Interferon beta-2) (IFN-beta-2) PROTEIN FAMILY: IL-6 superfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the IL-6 superfamily. FUNCTION: Cytokine with a wide variety of biological functions in immunity, tissue regeneration, and metabolism. Binds to IL6R, then the complex associates to the signaling subunit IL6ST/gp130 to trigger the intracellular IL6-signaling pathway (Probable). The interaction with the membrane-bound IL6R and IL6ST stimulates 'classic signaling', whereas the binding of IL6 and soluble IL6R to IL6ST stimulates 'trans-signaling'. Alternatively, 'cluster signaling' occurs when membrane-bound IL6:IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells (Probable).; FUNCTION: IL6 is a potent inducer of the acute phase response. Rapid production of IL6 contributes to host defense during infection and tissue injury, but excessive IL6 synthesis is involved in disease pathology. In the innate immune response, is synthesized by myeloid cells, such as macrophages and dendritic cells, upon recognition of pathogens through toll-like receptors (TLRs) at the site of infection or tissue injury (Probable). In the adaptive immune response, is required for the differentiation of B cells into immunoglobulin-secreting cells. Plays a major role in the differentiation of CD4(+) T cell subsets. Essential factor for the development of T follicular helper (Tfh) cells that are required for the induction of germinal-center formation. Required to drive naive CD4(+) T cells to the Th17 lineage. Also required for proliferation of myeloma cells and the survival of plasmablast cells (By similarity).; FUNCTION: Acts as an essential factor in bone homeostasis and on vessels directly or indirectly by induction of VEGF, resulting in increased angiogenesis activity and vascular permeability. Induces, through 'trans-signaling' and synergistically with IL1B and TNF, the production of VEGF. Involved in metabolic controls, is discharged into the bloodstream after muscle contraction increasing lipolysis and improving insulin resistance. 'Trans-signaling' in central nervous system also regulates energy and glucose homeostasis (By similarity). Mediates, through GLP-1, crosstalk between insulin-sensitive tissues, intestinal L cells and pancreatic islets to adapt to changes in insulin demand (By similarity). Also acts as a myokine (Probable). Plays a protective role during liver injury, being required for maintenance of tissue regeneration (By similarity). Also has a pivotal role in iron metabolism by regulating HAMP/hepcidin expression upon inflammation or bacterial infection. Through activation of IL6ST-YAP-NOTCH pathway, induces inflammation-induced epithelial regeneration (By similarity). KEYWORDS: 3D-structure;Acute phase;Cytokine;Direct protein sequencing;Disulfide bond;Glycoprotein;Growth factor;Phosphoprotein;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted " P05458,"PROTEIN NAMES: Protease 3 (Pitrilysin) (Protease III) (Protease pi) PROTEIN FAMILY: Peptidase M16 family ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the peptidase M16 family. FUNCTION: Endopeptidase that degrades small peptides of less than 7 kDa, such as glucagon and insulin. KEYWORDS: 3D-structure;Hydrolase;Magnesium;Metal-binding;Metalloprotease;Periplasm;Protease;Reference proteome;Signal;Zinc SUBCELLULAR LOCATION: Periplasm. " P05546,"PROTEIN NAMES: Heparin cofactor 2 (Heparin cofactor II) (HC-II) (Protease inhibitor leuserpin-2) (HLS2) (Serpin D1) PROTEIN FAMILY: Serpin family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the serpin family. FUNCTION: Thrombin inhibitor activated by the glycosaminoglycans, heparin or dermatan sulfate. In the presence of the latter, HC-II becomes the predominant thrombin inhibitor in place of antithrombin III (AT-III). Also inhibits chymotrypsin, but in a glycosaminoglycan-independent manner.; FUNCTION: Peptides at the N-terminal of HC-II have chemotactic activity for both monocytes and neutrophils. KEYWORDS: 3D-structure;Blood coagulation;Chemotaxis;Direct protein sequencing;Disease variant;Glycoprotein;Hemostasis;Heparin-binding;Phosphoprotein;Protease inhibitor;Reference proteome;Repeat;Serine protease inhibitor;Signal;Sulfation;Thrombophilia " P05630,"PROTEIN NAMES: ATP synthase subunit delta, mitochondrial (ATP synthase F1 subunit delta) (F-ATPase delta subunit) PROTEIN FAMILY: ATPase epsilon chain family ORGANISM: Bos taurus (Bovine) SIMILARITY: Belongs to the ATPase epsilon chain family. FUNCTION: Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP turnover in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(1) domain and of the central stalk which is part of the complex rotary element. Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. KEYWORDS: 3D-structure;Acetylation;ATP synthesis;CF(1);Direct protein sequencing;Hydrogen ion transport;Ion transport;Membrane;Mitochondrion;Mitochondrion inner membrane;Reference proteome;Transit peptide;Transport SUBCELLULAR LOCATION: Mitochondrion. Mitochondrion inner membrane. " P05655,"PROTEIN NAMES: Levansucrase (Beta-D-fructofuranosyl transferase) (Fructosyltransferase) (FTF) (Sucrose 6-fructosyl transferase) PROTEIN FAMILY: Glycosyl hydrolase 68 family ORGANISM: Bacillus subtilis (strain 168) SIMILARITY: Belongs to the glycosyl hydrolase 68 family. FUNCTION: Catalyzes the synthesis of levan, a fructose polymer, by transferring the fructosyl moiety from sucrose to a growing acceptor molecule. Also displays sucrose hydrolase activity. At low sucrose concentrations, functions as an hydrolase with water as acceptor, whereas at higher substrate concentrations it adds fructosyl units to a growing levan chain. KEYWORDS: 3D-structure;Calcium;Carbohydrate metabolism;Glycosyltransferase;Metal-binding;Reference proteome;Secreted;Signal;Transferase SUBCELLULAR LOCATION: Secreted " P05656,"PROTEIN NAMES: Levanase (Beta-D-fructofuranosidase) (Exo-beta-D-fructosidase) (Exo-levanase) PROTEIN FAMILY: Glycosyl hydrolase 32 family ORGANISM: Bacillus subtilis (strain 168) SIMILARITY: Belongs to the glycosyl hydrolase 32 family. FUNCTION: Exo-fructosidase that can hydrolyze both levan and inulin, leading to the production of free fructose. Is also able to hydrolyze sucrose and to a small extent raffinose, but not melezitose, stachylose, cellobiose, maltose, and lactose. KEYWORDS: 3D-structure;Carbohydrate metabolism;Glycosidase;Hydrolase;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted MISCELLANEOUS: Levanase cannot be detected in the wild-type B.subtilis but is mostly secreted into the culture medium by SacL mutants, especially at the end of the exponential growth phase." P05806,PROTEIN NAMES: Bacillolysin (Neutral protease) PROTEIN FAMILY: Peptidase M4 family ORGANISM: Bacillus cereus SIMILARITY: Belongs to the peptidase M4 family. FUNCTION: Extracellular zinc metalloprotease. KEYWORDS: 3D-structure;Calcium;Direct protein sequencing;Hydrolase;Metal-binding;Metalloprotease;Protease;Secreted;Signal;Zinc SUBCELLULAR LOCATION: Secreted. P05817,PROTEIN NAMES: Prestalk D11 protein (Adhesion modulation protein A) ORGANISM: Dictyostelium discoideum (Social amoeba) KEYWORDS: Developmental protein;Reference proteome;Repeat;Signal P06101,"PROTEIN NAMES: Hsp90 co-chaperone Cdc37 (Cell division control protein 37) (Hsp90 chaperone protein kinase-targeting subunit) PROTEIN FAMILY: CDC37 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the CDC37 family. FUNCTION: Co-chaperone that binds to numerous kinases and promotes their interaction with the Hsp90 complex, resulting in stabilization and promotion of their activity. Involved in both the HOG and the PKC MAP kinase signaling cascade necessary for adaptation to stress conditions due to high osmolarity or cell wall perturbation. KEYWORDS: Cell cycle;Cell division;Chaperone;Cytoplasm;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cytoplasm MISCELLANEOUS: Present with 10200 molecules/cell in log phase SD medium." P06127,PROTEIN NAMES: T-cell surface glycoprotein CD5 (Lymphocyte antigen T1/Leu-1) (CD antigen CD5) ORGANISM: Homo sapiens (Human) FUNCTION: May act as a receptor in regulating T-cell proliferation. KEYWORDS: 3D-structure;Cell membrane;Disulfide bond;Glycoprotein;Membrane;Phosphoprotein;Reference proteome;Repeat;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. P06181,"PROTEIN NAMES: Ligninase H8 (Diarylpropane peroxidase) (Lignin peroxidase) PROTEIN FAMILY: Peroxidase family, Ligninase subfamily ORGANISM: Phanerodontia chrysosporium (White-rot fungus) (Sporotrichum pruinosum) SIMILARITY: Belongs to the peroxidase family. Ligninase subfamily. FUNCTION: Depolymerization of lignin. Catalyzes the C(alpha)-C(beta) cleavage of the propyl side chains of lignin. PATHWAY: Secondary metabolite metabolism; lignin degradation. KEYWORDS: 3D-structure;Calcium;Cleavage on pair of basic residues;Direct protein sequencing;Disulfide bond;Glycoprotein;Heme;Hydrogen peroxide;Iron;Lignin degradation;Metal-binding;Oxidoreductase;Peroxidase;Signal;Zymogen " P06202,"PROTEIN NAMES: Periplasmic oligopeptide-binding protein OppA PROTEIN FAMILY: Bacterial solute-binding protein 5 family ORGANISM: Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) SIMILARITY: Belongs to the bacterial solute-binding protein 5 family. FUNCTION: Part of the ABC transporter complex OppABCDF involved in the uptake of oligopeptides, including the cell wall murein tripeptide L-alanyl-gamma-D-glutamyl-meso-diaminopimelate. Plays an important nutritional role and is involved in the recycling of cell wall peptides. Binds peptides containing from two to five amino acid residues regardless of their sequence. Also binds cell wall peptides, such as L-alanyl-gamma-D-glutamyl-meso-diaminopimelate. KEYWORDS: 3D-structure;Direct protein sequencing;Disulfide bond;Peptide transport;Periplasm;Protein transport;Reference proteome;Signal;Transport SUBCELLULAR LOCATION: Periplasm MISCELLANEOUS: Isothermal titration calorimetric measurements of the binding of lysine-containing peptides of different lengths to OppA show that the dipeptide Lys-Lys is bound with 60-fold lower affinity than related tri- and tetrapeptides (Lys-Lys-Lys and Lys-Lys-Lys-Ala, respectively)." P06400,"PROTEIN NAMES: Retinoblastoma-associated protein (p105-Rb) (p110-RB1) (pRb) (Rb) (pp110) PROTEIN FAMILY: Retinoblastoma protein (RB) family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the retinoblastoma protein (RB) family. FUNCTION: Tumor suppressor that is a key regulator of the G1/S transition of the cell cycle. The hypophosphorylated form binds transcription regulators of the E2F family, preventing transcription of E2F-responsive genes. Both physically blocks E2Fs transactivating domain and recruits chromatin-modifying enzymes that actively repress transcription. Cyclin and CDK-dependent phosphorylation of RB1 induces its dissociation from E2Fs, thereby activating transcription of E2F responsive genes and triggering entry into S phase. RB1 also promotes the G0-G1 transition upon phosphorylation and activation by CDK3/cyclin-C. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity).; FUNCTION: (Microbial infection) In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity. KEYWORDS: 3D-structure;Acetylation;Cell cycle;Chromatin regulator;Direct protein sequencing;Disease variant;DNA-binding;Host-virus interaction;Methylation;Nucleus;Phosphoprotein;Reference proteome;Repressor;Transcription;Transcription regulation;Tumor suppressor SUBCELLULAR LOCATION: Nucleus Note=During keratinocyte differentiation, acetylation by KAT2B/PCAF is required for nuclear localization. " P06606,"PROTEIN NAMES: Tubulin alpha-4 chain PROTEIN FAMILY: Tubulin family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the tubulin family. FUNCTION: Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin. KEYWORDS: Cytoplasm;Cytoskeleton;GTP-binding;Hydrolase;Magnesium;Metal-binding;Microtubule;Nucleotide-binding;Reference proteome SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton. " P06652,PROTEIN NAMES: M-phase inducer phosphatase (Mitosis initiation protein) (P80) PROTEIN FAMILY: MPI phosphatase family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the MPI phosphatase family. FUNCTION: Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic and meiotic progression. Directly dephosphorylates cdc2 and stimulates its kinase activity (By similarity). Required for the G2/M transition of the cell cycle. Required for induction of meiosis II. KEYWORDS: Cell cycle;Cell division;Cytoplasm;Hydrolase;Mitosis;Nucleus;Phosphoprotein;Protein phosphatase;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Nucleus Note=Accumulates in the nucleus in cycling cells; nuclear localization is the highest in G2. P06838,"PROTEIN NAMES: DNA repair protein RAD10 PROTEIN FAMILY: ERCC1/RAD10/SWI10 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the ERCC1/RAD10/SWI10 family. FUNCTION: Involved in nucleotide excision repair of DNA damaged with UV light, bulky adducts, or cross-linking agents. Along with RAD1 forms an endonuclease that specifically degrades single-stranded DNA. KEYWORDS: DNA damage;DNA repair;DNA-binding;Endonuclease;Hydrolase;Nuclease;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus. " P07102,"PROTEIN NAMES: Phytase AppA (6-phytase) (Histidine acid phosphatase phytase) (HAP phytase) (Myo-inositol hexakisphosphate phosphohydrolase) (Phosphoanhydride phosphatase) (pH 2.5 acid phosphatase) (Acid phosphatase) PROTEIN FAMILY: Histidine acid phosphatase family ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the histidine acid phosphatase family. FUNCTION: Catalyzes the hydrolysis of phytate (or myo-inositol hexakisphosphate, an indigestible organic form of phosphorus that is found in many plant tissues) to myo-inositol and inorganic phosphate. Dephosphorylates phytate in a stereospecific way by sequential removal of phosphate groups to produce myo-inositol 2-monophosphate. Also shows phosphoanhydride phosphatase activity and hydrolyzes the distal phosphoryl residues of GTP, the 5'-beta-phosphoryl residue of the regulatory nucleotide ppGpp and tripolyphosphates. Does not split most phosphomonoesters with the exception of the synthetic substrate p-nitrophenyl phosphate (pNPP), 2,3-bisphosphoglycerate and fructose 1,6-bisphosphate. KEYWORDS: 3D-structure;Direct protein sequencing;Disulfide bond;Hydrolase;Periplasm;Reference proteome;Signal SUBCELLULAR LOCATION: Periplasm " P07103,"PROTEIN NAMES: Endoglucanase Z (Cellulase Z) (Endo-1,4-beta-glucanase Z) (EGZ) PROTEIN FAMILY: Glycosyl hydrolase 5 (cellulase A) family ORGANISM: Dickeya dadantii (strain 3937) (Erwinia chrysanthemi (strain 3937)) SIMILARITY: Belongs to the glycosyl hydrolase 5 (cellulase A) family. FUNCTION: Represents 97% of the global cellulase activity. KEYWORDS: 3D-structure;Carbohydrate metabolism;Cellulose degradation;Disulfide bond;Glycosidase;Hydrolase;Polysaccharide degradation;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted. " P07112,"PROTEIN NAMES: Mobilization protein A (DNA strand transferase) [Includes: DNA relaxase (DNA nickase); DNA primase ] PROTEIN FAMILY: MobA/MobL family ORGANISM: Escherichia coli SIMILARITY: Belongs to the MobA/MobL family. FUNCTION: Part of the relaxosome complex that is responsible for plasmid transfer during conjugation. Locally unwinds DNA and catalyzes the cleavage of one of the DNA strands at oriT. The cleaved strand is then transferred through the dedicated type IV secretion apparatus. MobA remains covalently linked at the 5' end of the strand, and once in the recipient cell, it probably catalyzes the rejoining of the two ends of the strand, re-forming the circular plasmid DNA. The primase activity of MobA is essential for the synthesis of primers that will initiate the DNA replication events necessary to form the double-stranded plasmid in the recipient cell. KEYWORDS: 3D-structure;Calcium;Coiled coil;Conjugation;Cytoplasm;Direct protein sequencing;DNA-binding;DNA-directed RNA polymerase;Isomerase;Magnesium;Manganese;Metal-binding;Mobility protein;Multifunctional enzyme;Plasmid;Topoisomerase;Transcription;Transferase SUBCELLULAR LOCATION: Cytoplasm " P07173,PROTEIN NAMES: Photosynthetic reaction center cytochrome c subunit (Cytochrome c558/c559) ORGANISM: Blastochloris viridis (Rhodopseudomonas viridis) FUNCTION: The reaction center of purple bacteria contains a tightly bound cytochrome molecule which re-reduces the photo oxidized primary electron donor. KEYWORDS: 3D-structure;Direct protein sequencing;Electron transport;Heme;Iron;Lipoprotein;Membrane;Metal-binding;Photosynthesis;Reaction center;Reference proteome;Signal;Transport SUBCELLULAR LOCATION: Cellular chromatophore membrane ; Lipid-anchor P07247,PROTEIN NAMES: Protein krueppel PROTEIN FAMILY: Krueppel C2H2-type zinc-finger protein family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein family. FUNCTION: Krueppel is a gap class segmentation protein. It is involved in the segmentation of the embryo and in the differentiation of the Malpighian tubules. KEYWORDS: Developmental protein;DNA-binding;Gap protein;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Repeat;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Note=Chromatin associated. P07250,"PROTEIN NAMES: Inositol polyphosphate multikinase (IPMK) (Arginine metabolism regulation protein III) (GLE1 synthetic lethal protein 3) (Inositol polyphosphate kinase 2) PROTEIN FAMILY: Inositol phosphokinase (IPK) family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the inositol phosphokinase (IPK) family. FUNCTION: Inositol phosphate kinase with both monophosphoinositol and diphosphoinositol polyphosphate synthase activities. Able to phosphorylate inositol 1,4,5-trisphosphate (Ins(1,4,5)P3) on both the carbon-3 and carbon-6 positions to synthesize inositol 1,3,4,5-tetrakisphosphate (Ins(1,3,4,5)P4) and inositol 1,4,5,6-tetrakisphosphate (Ins(1,4,5,6)P4), and then to subsequently phosphorylate and convert either isomer of InsP4 to inositol 1,3,4,5,6-pentakisphosphate (Ins(1,3,4,5,6)P5). Its predominant in vivo catalytic function is to convert Ins(1,4,5)P3 to Ins(1,4,5,6)P4 to Ins(1,3,4,5,6)P5 via 6- and 3-kinase activities. It can also use Ins(1,3,4,5,6)P5 as a substrate and act as a diphosphoinositol polyphosphate synthase to generate two different isomers of PP-InsP4. Has also a role in transcription regulation. Forms a complex with ARG80, ARG81 and MCM1 (ArgR-MCM1), which coordinates the expression of arginine anabolic and catabolic genes in response to arginine. Recruits ARG80 and MCM21 to stabilize them. Neither the kinase activity nor inositol phosphates are required for the formation of ArgR-MCM1 transcriptional complexes on DNA promoter elements and the control of arginine metabolism. In contrast, only the catalytic activity is required for PHO gene repression by phosphate and for NCR gene activation in response to nitrogen availability, indicating a role for inositol pyrophosphates in these controls. Inositol polyphosphates may be involved in the regulation of chromatin remodeling of transcription. Regulates nuclear mRNA export via inositol phosphate metabolism. Also has lipid kinase activity, transforming the lipid inositol phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) into phosphatidylinositol 3,4,5-trisphosphate (PI(3,4,5)P3) in the nucleus. Its kinase activity is necessary for the propagation of most [PSI+] prion variants. KEYWORDS: 3D-structure;Acetylation;Arginine metabolism;ATP-binding;Calcium;Kinase;Metal-binding;Nucleotide-binding;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation;Transferase SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: The expression of this protein is not effected by the presence of arginine.; MISCELLANEOUS: Present with 2720 molecules/cell in log phase SD medium." P07256,"PROTEIN NAMES: Cytochrome b-c1 complex subunit 1, mitochondrial (Complex III subunit 1) (Core protein I) (Ubiquinol-cytochrome c oxidoreductase core protein 1) (Ubiquinol-cytochrome c reductase 44 kDa protein) PROTEIN FAMILY: Peptidase M16 family, UQCRC1/QCR1 subfamily ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the peptidase M16 family. UQCRC1/QCR1 subfamily. FUNCTION: Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. The cytochrome b-c1 complex catalyzes electron transfer from ubiquinol to cytochrome c, linking this redox reaction to translocation of protons across the mitochondrial inner membrane, with protons being carried across the membrane as hydrogens on the quinol. In the process called Q cycle, 2 protons are consumed from the matrix, 4 protons are released into the intermembrane space and 2 electrons are passed to cytochrome c. KEYWORDS: 3D-structure;Electron transport;Membrane;Mitochondrion;Mitochondrion inner membrane;Reference proteome;Respiratory chain;Transit peptide;Transport SUBCELLULAR LOCATION: Mitochondrion inner membrane ; Peripheral membrane protein ; Matrix side MISCELLANEOUS: Present with 19300 molecules/cell in log phase SD medium." P07257,"PROTEIN NAMES: Cytochrome b-c1 complex subunit 2, mitochondrial (Complex III subunit 2) (Core protein II) (Ubiquinol-cytochrome c oxidoreductase core protein 2) PROTEIN FAMILY: Peptidase M16 family, UQCRC2/QCR2 subfamily ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the peptidase M16 family. UQCRC2/QCR2 subfamily. FUNCTION: Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. The cytochrome b-c1 complex catalyzes electron transfer from ubiquinol to cytochrome c, linking this redox reaction to translocation of protons across the mitochondrial inner membrane, with protons being carried across the membrane as hydrogens on the quinol. In the process called Q cycle, 2 protons are consumed from the matrix, 4 protons are released into the intermembrane space and 2 electrons are passed to cytochrome c. KEYWORDS: 3D-structure;Direct protein sequencing;Electron transport;Membrane;Mitochondrion;Mitochondrion inner membrane;Phosphoprotein;Reference proteome;Respiratory chain;Transit peptide;Transport SUBCELLULAR LOCATION: Mitochondrion inner membrane ; Peripheral membrane protein ; Matrix side MISCELLANEOUS: Present with 35700 molecules/cell in log phase SD medium." P07260,PROTEIN NAMES: Eukaryotic translation initiation factor 4E (eIF-4E) (eIF4E) (eIF-4F 25 kDa subunit) (mRNA cap-binding protein) PROTEIN FAMILY: Eukaryotic initiation factor 4E family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the eukaryotic initiation factor 4E family. FUNCTION: Recognizes and binds the 7-methylguanosine (m7G)-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. KEYWORDS: 3D-structure;Cytoplasm;Initiation factor;Isopeptide bond;Nucleus;Phosphoprotein;Protein biosynthesis;Reference proteome;RNA-binding;Translation regulation;Ubl conjugation SUBCELLULAR LOCATION: Cytoplasm Nucleus MISCELLANEOUS: Present with 14200 molecules/cell in log phase SD medium. P07269,"PROTEIN NAMES: Regulatory protein PHO2 (General regulatory factor 10) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Regulator in phosphate metabolism and acts as a derepressor of another central regulator PHO5. Binds to the upstream activator sequence (UAS) of PHO5. It also binds to the TRP4, HIS4, and CYC1 promoters. KEYWORDS: DNA-binding;Homeobox;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus. MISCELLANEOUS: Present with 6420 molecules/cell in log phase SD medium." P07273,"PROTEIN NAMES: Transcription elongation factor S-II (DNA strand transfer protein alpha) (STP-alpha) (DNA strand transferase 1) (Pyrimidine pathway regulatory protein 2) PROTEIN FAMILY: TFS-II family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the TFS-II family. FUNCTION: Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.; FUNCTION: Can promote the transfer of one strand of a double-stranded DNA molecule to a homologous single strand and thus may be involved in recombination. KEYWORDS: 3D-structure;Direct protein sequencing;DNA-binding;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus. MISCELLANEOUS: S-II binds to RNA-polymerase II in the absence of transcription.; MISCELLANEOUS: Present with 6260 molecules/cell in log phase SD medium." P07277,"PROTEIN NAMES: Mevalonate kinase (MK) (MvK) (Ergosterol biosynthesis protein 12) (Regulation of autonomous replication protein 1) PROTEIN FAMILY: GHMP kinase family, Mevalonate kinase subfamily ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the GHMP kinase family. Mevalonate kinase subfamily. FUNCTION: Mevalonate kinase; part of the second module of ergosterol biosynthesis pathway that includes the middle steps of the pathway. ERG12 converts mevalonate into 5-phosphomevalonate. The second module is carried out in the vacuole and involves the formation of farnesyl diphosphate, which is also an important intermediate in the biosynthesis of ubiquinone, dolichol, heme and prenylated proteins. Activity by the mevalonate kinase ERG12 first converts mevalonate into 5-phosphomevalonate. 5-phosphomevalonate is then further converted to 5-diphosphomevalonate by the phosphomevalonate kinase ERG8. The diphosphomevalonate decarboxylase MVD1/ERG19 then produces isopentenyl diphosphate. The isopentenyl-diphosphate delta-isomerase IDI1 then catalyzes the 1,3-allylic rearrangement of the homoallylic substrate isopentenyl (IPP) to its highly electrophilic allylic isomer, dimethylallyl diphosphate (DMAPP). Finally the farnesyl diphosphate synthase ERG20 catalyzes the sequential condensation of isopentenyl pyrophosphate with dimethylallyl pyrophosphate, and then with the resultant geranylpyrophosphate to the ultimate product farnesyl pyrophosphate. PATHWAY: Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via mevalonate pathway; isopentenyl diphosphate from (R)-mevalonate: step 1/3. KEYWORDS: ATP-binding;Cytoplasm;Kinase;Lipid biosynthesis;Lipid metabolism;Magnesium;Metal-binding;Nucleotide-binding;Reference proteome;Steroid biosynthesis;Steroid metabolism;Sterol biosynthesis;Sterol metabolism;Transferase SUBCELLULAR LOCATION: Cytoplasm, cytosol MISCELLANEOUS: Present with 3300 molecules/cell in log phase SD medium." P07284,"PROTEIN NAMES: Serine--tRNA ligase, cytoplasmic (Seryl-tRNA synthetase) (SerRS) (Seryl-tRNA(Ser) synthetase) PROTEIN FAMILY: Class-II aminoacyl-tRNA synthetase family, Type-1 seryl-tRNA synthetase subfamily ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily. FUNCTION: Catalyzes the attachment of serine to tRNA(Ser) in a two-step reaction: serine is first activated by ATP to form Ser-AMP and then transferred to the acceptor end of tRNA(Ser). KEYWORDS: Aminoacyl-tRNA synthetase;ATP-binding;Cytoplasm;Direct protein sequencing;Ligase;Nucleotide-binding;Protein biosynthesis;Reference proteome SUBCELLULAR LOCATION: Cytoplasm, cytosol " P07334,"PROTEIN NAMES: Mitosis inducer protein kinase cdr1 (Protein kinase nim1) PROTEIN FAMILY: Protein kinase superfamily, CAMK Ser/Thr protein kinase family, NIM1 subfamily ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. NIM1 subfamily. FUNCTION: This protein, a dose-dependent mitotic inducer, appears to function as a negative regulator of mitosis inhibitor wee1 by phosphorylating and inactivating it. KEYWORDS: ATP-binding;Cell cycle;Cell division;Kinase;Mitosis;Nucleotide-binding;Phosphoprotein;Reference proteome;Serine/threonine-protein kinase;Transferase " P07464,"PROTEIN NAMES: Galactoside O-acetyltransferase (GAT) (Acetyl-CoA:galactoside 6-O-acetyltransferase) (Thiogalactoside acetyltransferase) (Thiogalactoside transacetylase) PROTEIN FAMILY: Transferase hexapeptide repeat family ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the transferase hexapeptide repeat family. FUNCTION: Catalyzes the CoA-dependent transfer of an acetyl group to the 6-O-methyl position of a range of galactosides, glucosides, and lactosides. May assist cellular detoxification by acetylating non-metabolizable pyranosides, thereby preventing their reentry into the cell (Probable). KEYWORDS: 3D-structure;Acyltransferase;Cytoplasm;Direct protein sequencing;Lactose biosynthesis;Reference proteome;Repeat;Transferase SUBCELLULAR LOCATION: Cytoplasm " P07548,"PROTEIN NAMES: Homeotic protein deformed PROTEIN FAMILY: Antp homeobox family, Deformed subfamily ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the Antp homeobox family. Deformed subfamily. FUNCTION: Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Homeotic protein controlling Drosophila head development. Transcriptional activator of the apoptotic activator protein rpr in cells at the maxillary/mandibular boundary. KEYWORDS: Developmental protein;DNA-binding;Homeobox;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus. " P07584,PROTEIN NAMES: Astacin (Crayfish small molecule proteinase) ORGANISM: Astacus astacus (Noble crayfish) (Astacus fluviatilis) FUNCTION: Metalloprotease. This protease prefers to cleave in front of small aliphatic residues (P1'). The presence of Lys or Arg in the P1 and P2 position yields high-turnover substrates. In the P3 position the enzyme prefers Pro > Val > Leu > Ala > Gly. KEYWORDS: 3D-structure;Direct protein sequencing;Disulfide bond;Hydrolase;Metal-binding;Metalloprotease;Protease;Signal;Zinc;Zymogen P07591,"PROTEIN NAMES: Thioredoxin M-type, chloroplastic (Trx-M) [Cleaved into: Thioredoxin M-type Mc; Thioredoxin M-type Md] PROTEIN FAMILY: Thioredoxin family, Plant M-type subfamily ORGANISM: Spinacia oleracea (Spinach) SIMILARITY: Belongs to the thioredoxin family. Plant M-type subfamily. FUNCTION: Participates in various redox reactions through the reversible oxidation of the active center dithiol to a disulfide. The M form is known to activate NADP-malate dehydrogenase. KEYWORDS: 3D-structure;Chloroplast;Direct protein sequencing;Disulfide bond;Electron transport;Plastid;Redox-active center;Reference proteome;Transit peptide;Transport SUBCELLULAR LOCATION: Plastid, chloroplast. " P07668,PROTEIN NAMES: Choline O-acetyltransferase (CHOACTase) (Choline acetylase) [Cleaved into: Choline O-acetyltransferase 67 kDa chain; Choline O-acetyltransferase 54 kDa chain; Choline O-acetyltransferase 13 kDa chain] PROTEIN FAMILY: Carnitine/choline acetyltransferase family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the carnitine/choline acetyltransferase family. FUNCTION: Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. KEYWORDS: Acyltransferase;Alternative splicing;Direct protein sequencing;Neurotransmitter biosynthesis;Reference proteome;Transferase P07686,"PROTEIN NAMES: Beta-hexosaminidase subunit beta (Beta-N-acetylhexosaminidase subunit beta) (Hexosaminidase subunit B) (Cervical cancer proto-oncogene 7 protein) (HCC-7) (N-acetyl-beta-glucosaminidase subunit beta) [Cleaved into: Beta-hexosaminidase subunit beta chain B; Beta-hexosaminidase subunit beta chain A] PROTEIN FAMILY: Glycosyl hydrolase 20 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the glycosyl hydrolase 20 family. FUNCTION: Hydrolyzes the non-reducing end N-acetyl-D-hexosamine and/or sulfated N-acetyl-D-hexosamine of glycoconjugates, such as the oligosaccharide moieties from proteins and neutral glycolipids, or from certain mucopolysaccharides. The isozyme B does not hydrolyze each of these substrates, however hydrolyzes efficiently neutral oligosaccharide. Only the isozyme A is responsible for the degradation of GM2 gangliosides in the presence of GM2A. During fertilization is responsible, at least in part, for the zona block to polyspermy. Present in the cortical granules of non-activated oocytes, is exocytosed during the cortical reaction in response to oocyte activation and inactivates the sperm galactosyltransferase-binding site, accounting for the block in sperm binding to the zona pellucida (By similarity). KEYWORDS: 3D-structure;Cytoplasmic vesicle;Direct protein sequencing;Disease variant;Disulfide bond;Gangliosidosis;Glycoprotein;Glycosidase;Hydrolase;Lipid metabolism;Lysosome;Neurodegeneration;Reference proteome;Signal;Zymogen SUBCELLULAR LOCATION: Lysosome Cytoplasmic vesicle, secretory vesicle, Cortical granule " P07834,"PROTEIN NAMES: Cell division control protein 4 (E3 ubiquitin ligase complex SCF subunit CDC4) (F-box protein CDC4) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes and binds to phosphorylated target proteins. Directs ubiquitination of the phosphorylated CDK inhibitor SIC1. Involved in the degradation of CDC6 together with CDC34/UBC3 and CDC53, and in restricting the degradation of FAR1 to the nucleus. Is essential for initiation of DNA replication and separation of the spindle pole bodies to form the poles of the mitotic spindle. It also plays a role in bud development, fusion of zygotic nuclei after conjugation and various aspects of sporulation. Required for HTA1-HTB1 locus transcription activation. Required for G1/S and G2/M transition. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: 3D-structure;Cell cycle;Cell division;Mitosis;Nucleus;Phosphoprotein;Reference proteome;Repeat;Sporulation;Ubl conjugation pathway;WD repeat SUBCELLULAR LOCATION: Nucleus " P07902,PROTEIN NAMES: Galactose-1-phosphate uridylyltransferase (Gal-1-P uridylyltransferase) (UDP-glucose--hexose-1-phosphate uridylyltransferase) PROTEIN FAMILY: Galactose-1-phosphate uridylyltransferase type 1 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the galactose-1-phosphate uridylyltransferase type 1 family. FUNCTION: Plays an important role in galactose metabolism. PATHWAY: Carbohydrate metabolism; galactose metabolism. KEYWORDS: 3D-structure;Alternative splicing;Carbohydrate metabolism;Cataract;Disease variant;Galactose metabolism;Metal-binding;Nucleotidyltransferase;Reference proteome;Transferase;Zinc P07986,"PROTEIN NAMES: Exoglucanase/xylanase [Includes: Exoglucanase (1,4-beta-cellobiohydrolase) (Beta-1,4-glycanase CEX) (Exocellobiohydrolase); Endo-1,4-beta-xylanase B (Xylanase B) ] PROTEIN FAMILY: Glycosyl hydrolase 10 (cellulase F) family ORGANISM: Cellulomonas fimi SIMILARITY: Belongs to the glycosyl hydrolase 10 (cellulase F) family. FUNCTION: Hydrolyzes both cellulose and xylan. Has also weak endoglucanase activity.; FUNCTION: The biological conversion of cellulose to glucose generally requires three types of hydrolytic enzymes: (1) Endoglucanases which cut internal beta-1,4-glucosidic bonds; (2) Exocellobiohydrolases that cut the disaccharide cellobiose from the non-reducing end of the cellulose polymer chain; (3) Beta-1,4-glucosidases which hydrolyze the cellobiose and other short cello-oligosaccharides to glucose. KEYWORDS: 3D-structure;Carbohydrate metabolism;Cellulose degradation;Disulfide bond;Glycosidase;Hydrolase;Multifunctional enzyme;Polysaccharide degradation;Repeat;Signal;Xylan degradation MISCELLANEOUS: The linker region (also termed 'hinge') may be a potential site for proteolysis." P07988,"PROTEIN NAMES: Pulmonary surfactant-associated protein B (SP-B) (18 kDa pulmonary-surfactant protein) (6 kDa protein) (Pulmonary surfactant-associated proteolipid SPL(Phe)) ORGANISM: Homo sapiens (Human) FUNCTION: Pulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter. KEYWORDS: 3D-structure;Direct protein sequencing;Disease variant;Disulfide bond;Gaseous exchange;Glycoprotein;Reference proteome;Repeat;Secreted;Signal;Surface film SUBCELLULAR LOCATION: Secreted, extracellular space, surface film. MISCELLANEOUS: Pulmonary surfactant consists of 90% lipid and 10% protein. There are 4 surfactant-associated proteins: 2 collagenous, carbohydrate-binding glycoproteins (SP-A and SP-D) and 2 small hydrophobic proteins (SP-B and SP-C)." P08138,"PROTEIN NAMES: Tumor necrosis factor receptor superfamily member 16 (Gp80-LNGFR) (Low affinity neurotrophin receptor p75NTR) (Low-affinity nerve growth factor receptor) (NGF receptor) (Low-affinity nerve growth factor receptor p75NGFR) (Low-affinity nerve growth factor receptor p75NGR) (p75 ICD) (CD antigen CD271) ORGANISM: Homo sapiens (Human) FUNCTION: Low affinity receptor which can bind to NGF, BDNF, NTF3, and NTF4. Forms a heterodimeric receptor with SORCS2 that binds the precursor forms of NGF, BDNF and NTF3 with high affinity, and has much lower affinity for mature NGF and BDNF. Plays an important role in differentiation and survival of specific neuronal populations during development (By similarity). Can mediate cell survival as well as cell death of neural cells. Plays a role in the inactivation of RHOA. Plays a role in the regulation of the translocation of GLUT4 to the cell surface in adipocytes and skeletal muscle cells in response to insulin, probably by regulating RAB31 activity, and thereby contributes to the regulation of insulin-dependent glucose uptake (By similarity). Necessary for the circadian oscillation of the clock genes BMAL1, PER1, PER2 and NR1D1 in the suprachiasmatic nucleus (SCmgetaN) of the brain and in liver and of the genes involved in glucose and lipid metabolism in the liver. KEYWORDS: 3D-structure;Alternative splicing;Apoptosis;Biological rhythms;Cell membrane;Cell projection;Developmental protein;Differentiation;Disulfide bond;Glycoprotein;Membrane;Neurogenesis;Phosphoprotein;Receptor;Reference proteome;Repeat;Signal;Synapse;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein Perikaryon Cell projection, growth cone Cell projection, dendritic spine " P08153,"PROTEIN NAMES: Transcriptional factor SWI5 ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Determines the mother-cell-specific transcription of the HO endonuclease gene that is responsible for the initiation of mating-type switching in yeast. Recognizes a specific sequence in the promoter of the HO gene. Activates EGT2 transcription in a concentration-dependent manner. Synthesized during G2 and early mitosis. KEYWORDS: 3D-structure;Activator;Cytoplasm;DNA-binding;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Repeat;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Cytoplasm Note=Nuclear in G1, cytoplasmic in S, G2 and M cell cycle phases. MISCELLANEOUS: Present with 688 molecules/cell in log phase SD medium." P08159,"PROTEIN NAMES: (R)-6-hydroxynicotine oxidase (6-hydroxy-D-nicotine oxidase) (6-HDNO) (D-6-hydroxynicotine oxidase) (DHNO) PROTEIN FAMILY: Oxygen-dependent FAD-linked oxidoreductase family ORGANISM: Paenarthrobacter nicotinovorans (Arthrobacter nicotinovorans) SIMILARITY: Belongs to the oxygen-dependent FAD-linked oxidoreductase family. FUNCTION: Involved in the degradation of D-nicotine. Catalyzes the oxidation of (R)-6-hydroxynicotine (6-hydroxy-D-nicotine) to 6-hydroxypseudooxynicotine. Oxidation of the pyrrolidine ring of (R)-6-hydroxynicotine leads to the formation of the optically inactive 6-hydroxy-N-methylmyosmine, which hydrolyzes spontaneously to 6-hydroxypseudooxynicotine. Acts with absolute stereospecificity on the D-form of 6-hydroxynicotine. Shows lower activity with (R)-6-hydroxynornicotine, and weak activity with (R)-4-(1-methylpyrrolidine-2-yl)phenol, (R)-6-chloronicotine and (R)-nicotine. PATHWAY: Alkaloid degradation; nicotine degradation; 6-hydroxypseudooxynicotine from nicotine (R-isomer route): step 2/2. KEYWORDS: 3D-structure;Alkaloid metabolism;Cytoplasm;FAD;Flavoprotein;Nucleotide-binding;Oxidoreductase;Plasmid SUBCELLULAR LOCATION: Cytoplasm " P08246,"PROTEIN NAMES: Neutrophil elastase (Bone marrow serine protease) (Elastase-2) (Human leukocyte elastase) (HLE) (Medullasin) (PMN elastase) PROTEIN FAMILY: Peptidase S1 family, Elastase subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the peptidase S1 family. Elastase subfamily. FUNCTION: Serine protease that modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis. Promotes cleavage of GSDMB, thereby inhibiting pyroptosis. Capable of killing E.coli but not S.aureus in vitro; digests outer membrane protein A (ompA) in E.coli and K.pneumoniae. KEYWORDS: 3D-structure;Cytoplasmic vesicle;Direct protein sequencing;Disease variant;Disulfide bond;Glycoprotein;Hydrolase;Protease;Reference proteome;Serine protease;Signal;Zymogen SUBCELLULAR LOCATION: Cytoplasmic vesicle, phagosome Note=Localized in phagolysosomes following ingestion of E.coli by neutrophils. " P08337,"PROTEIN NAMES: 8-oxo-dGTP diphosphatase (8-oxo-dGTPase) (7,8-dihydro-8-oxoguanine-triphosphatase) (Mutator protein MutT) (dGTP pyrophosphohydrolase) PROTEIN FAMILY: Nudix hydrolase family ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the Nudix hydrolase family. FUNCTION: Specifically hydrolyzes both 8-oxo-deoxyguanosine triphosphate (8-oxo-dGTP) and 8-oxo-guanosine triphosphate (8-oxo-GTP) to the related monophosphates, thereby cleaning up the nucleotide pools and preventing misincorporation of 8-oxoGua into DNA and RNA. It prevents replicational errors by removing an oxidatively damaged form of guanine (8-oxo-dGTP) from DNA and the nucleotide pool. 8-oxo-dGTP can be inserted opposite dA and dC residues of template DNA with almost equal efficiency thus leading to A.T to G.C transversions. MutT may also ensure transcriptional fidelity, removing 8-oxo-GTP from the ribonucleotide triphosphate pool. However, due to the lower efficiency of RNA polymerase 8-oxo-GTP incorporation, MutT is probably not a major contributor to transcriptional fidelity. It also hydrolyzes 8-oxo-dGDP and 8-oxo-GDP to their monophosphate form. In vitro, can also use dGTP, dGDP and other various nucleoside di- and triphosphates, with much lower efficiency. Works cooperatively with MutM and MutY to prevent accumulation in the DNA of oxidized guanine residues. KEYWORDS: 3D-structure;Direct protein sequencing;DNA damage;DNA repair;DNA replication;Hydrolase;Magnesium;Metal-binding;Mutator protein;Reference proteome " P08397,"PROTEIN NAMES: Porphobilinogen deaminase (PBG-D) (Hydroxymethylbilane synthase) (HMBS) (Pre-uroporphyrinogen synthase) PROTEIN FAMILY: HMBS family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the HMBS family. FUNCTION: As part of the heme biosynthetic pathway, catalyzes the sequential polymerization of four molecules of porphobilinogen to form hydroxymethylbilane, also known as preuroporphyrinogen. Catalysis begins with the assembly of the dipyrromethane cofactor by the apoenzyme from two molecules of porphobilinogen or from preuroporphyrinogen. The covalently linked cofactor acts as a primer, around which the tetrapyrrole product is assembled. In the last step of catalysis, the product, preuroporphyrinogen, is released, leaving the cofactor bound to the holodeaminase intact. PATHWAY: Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 2/4. KEYWORDS: 3D-structure;Acetylation;Alternative splicing;Cytoplasm;Direct protein sequencing;Disease variant;Heme biosynthesis;Phosphoprotein;Porphyrin biosynthesis;Reference proteome;Transferase SUBCELLULAR LOCATION: Cytoplasm " P08425,"PROTEIN NAMES: Phenylalanine--tRNA ligase, mitochondrial (Phenylalanyl-tRNA synthetase) (PheRS) PROTEIN FAMILY: Class-II aminoacyl-tRNA synthetase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family. FUNCTION: Is responsible for the charging of tRNA(Phe) with phenylalanine in mitochondrial translation. KEYWORDS: Aminoacyl-tRNA synthetase;ATP-binding;Ligase;Mitochondrion;Nucleotide-binding;Protein biosynthesis;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Mitochondrion matrix MISCELLANEOUS: Present with 1800 molecules/cell in log phase SD medium." P08458,"PROTEIN NAMES: Sporulation-specific protein 1 PROTEIN FAMILY: Protein kinase superfamily, STE Ser/Thr protein kinase family, STE20 subfamily ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. FUNCTION: Serine/threonine protein kinase required for spore wall development. KEYWORDS: ATP-binding;Cytoplasm;Kinase;Meiosis;Nucleotide-binding;Nucleus;Reference proteome;Serine/threonine-protein kinase;Sporulation;Transferase SUBCELLULAR LOCATION: Nucleus Cytoplasm " P08466,PROTEIN NAMES: Mitochondrial nuclease PROTEIN FAMILY: DNA/RNA non-specific endonuclease family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the DNA/RNA non-specific endonuclease family. FUNCTION: This enzyme has both RNase and DNase activity. KEYWORDS: Endonuclease;Hydrolase;Magnesium;Manganese;Membrane;Metal-binding;Mitochondrion;Mitochondrion inner membrane;Nuclease;Reference proteome SUBCELLULAR LOCATION: Mitochondrion inner membrane. MISCELLANEOUS: Present with 3870 molecules/cell in log phase SD medium.; MISCELLANEOUS: The active site contains 1 hydrated divalent metal cation that has only 1 direct interaction with the protein; all other interactions are via water molecules. P08524,"PROTEIN NAMES: Farnesyl pyrophosphate synthase (FPP synthase) (FPS) ((2E,6E)-farnesyl diphosphate synthase) (Dimethylallyltranstransferase) (Farnesyl diphosphate synthase) (Geranyltranstransferase) PROTEIN FAMILY: FPP/GGPP synthase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the FPP/GGPP synthase family. FUNCTION: Farnesyl pyrophosphate synthase; part of the second module of ergosterol biosynthesis pathway that includes the middle steps of the pathway. ERG20 catalyzes the sequential condensation of isopentenyl pyrophosphate with dimethylallyl pyrophosphate, and then with the resultant geranylpyrophosphate to the ultimate product farnesyl pyrophosphate. The second module is carried out in the vacuole and involves the formation of farnesyl diphosphate, which is also an important intermediate in the biosynthesis of ubiquinone, dolichol, heme and prenylated proteins. Activity by the mevalonate kinase ERG12 first converts mevalonate into 5-phosphomevalonate. 5-phosphomevalonate is then further converted to 5-diphosphomevalonate by the phosphomevalonate kinase ERG8. The diphosphomevalonate decarboxylase MVD1/ERG19 then produces isopentenyl diphosphate. The isopentenyl-diphosphate delta-isomerase IDI1 then catalyzes the 1,3-allylic rearrangement of the homoallylic substrate isopentenyl (IPP) to its highly electrophilic allylic isomer, dimethylallyl diphosphate (DMAPP). Finally the farnesyl diphosphate synthase ERG20 catalyzes the sequential condensation of isopentenyl pyrophosphate with dimethylallyl pyrophosphate, and then with the resultant geranylpyrophosphate to the ultimate product farnesyl pyrophosphate. PATHWAY: Isoprenoid biosynthesis; farnesyl diphosphate biosynthesis; farnesyl diphosphate from geranyl diphosphate and isopentenyl diphosphate: step 1/1.; PATHWAY: Isoprenoid biosynthesis; geranyl diphosphate biosynthesis; geranyl diphosphate from dimethylallyl diphosphate and isopentenyl diphosphate: step 1/1. KEYWORDS: Isoprene biosynthesis;Lipid biosynthesis;Lipid metabolism;Magnesium;Metal-binding;Reference proteome;Transferase " P08539,"PROTEIN NAMES: Guanine nucleotide-binding protein alpha-1 subunit (GP1-alpha) PROTEIN FAMILY: G-alpha family, G(q) subfamily ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the G-alpha family. G(q) subfamily. FUNCTION: Alpha subunit of the heterotrimeric guanine nucleotide-binding protein (G protein) that mediates mating pheromone signal transduction. Binding of alpha-factor or a-factor to its cognate transmembrane receptor STE2 and STE3, respectively, allows the receptor to serve as a guanine nucleotide exchange factor (GEF) on GPA1. The exchange of GDP for GTP on the G protein alpha subunit alters its interaction with the G protein beta subunit STE4, leading to dissociation of the G protein beta-gamma dimer STE4-STE18. The dissociated subunits activate downstream effectors to activate the mating response pathway and induce changes necessary to produce mating-competent cells. STE4-STE18 activate the downstream pheromone signaling MAP kinase cascade leading to expression of mating-specific genes, inducing cell cycle arrest in G1, promoting polarized cell growth to form mating projections (shmoos), and establishing the changes in plasma membrane, cell wall and nuclear envelope to permit cell-cell fusion (plasmogamy) and fusion of the two haploid nuclei (karyogamy). GPA1 transmits a signal that requires direct binding to the effector enzyme PI3K located at the endosome, promoting increased PI3 production. The intrinsic GTPase activity of GPA1 determines the duration of signaling, and is dramatically accelerated by the RGS protein SST2. In unstimulated cells, GDP-bound GPA1 sequesters the G protein beta-gamma subunit STE4-STE18, preventing it from activating the downstream effectors. Also down-regulates the signal by inhibiting the pheromone-induced accumulation of FUS3 in the nucleus. KEYWORDS: 3D-structure;Cell membrane;Endosome;GTP-binding;Isopeptide bond;Lipoprotein;Magnesium;Membrane;Metal-binding;Myristate;Nucleotide-binding;Palmitate;Pheromone response;Reference proteome;Transducer;Ubl conjugation SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor; Cytoplasmic side. Endosome membrane; Lipid-anchor; Cytoplasmic side. Note=Localizes predominantly to the plasma membrane in its inactive, GDP-bound form, and is directed to endosomes once in its active, GTP-bound form. Concentrates at the tip of the mating projections. MISCELLANEOUS: Present with 9920 molecules/cell in log phase SD medium." P08571,"PROTEIN NAMES: Monocyte differentiation antigen CD14 (Myeloid cell-specific leucine-rich glycoprotein) (CD antigen CD14) [Cleaved into: Monocyte differentiation antigen CD14, urinary form; Monocyte differentiation antigen CD14, membrane-bound form] ORGANISM: Homo sapiens (Human) FUNCTION: Coreceptor for bacterial lipopolysaccharide. In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway. Binds electronegative LDL (LDL(-)) and mediates the cytokine release induced by LDL(-). KEYWORDS: 3D-structure;Cell membrane;Direct protein sequencing;Disulfide bond;Glycoprotein;Golgi apparatus;GPI-anchor;Immunity;Inflammatory response;Innate immunity;Leucine-rich repeat;Lipoprotein;Membrane;Reference proteome;Repeat;Secreted;Signal SUBCELLULAR LOCATION: Cell membrane ; Lipid-anchor, GPI-anchor Secreted Membrane raft Golgi apparatus Note=Secreted forms may arise by cleavage of the GPI anchor. " P08587,"PROTEIN NAMES: Protein-glutamine gamma-glutamyltransferase 2 (Guinea pig liver transglutaminase) (Isopeptidase TGM2) (Protein-glutamine deamidase TGM2) (Protein-glutamine dopaminyltransferase TGM2) (Protein-glutamine histaminyltransferase TGM2) (Protein-glutamine noradrenalinyltransferase TGM2) (Protein-glutamine serotonyltransferase TGM2) (Tissue transglutaminase) (tTG) (tTgase) (Transglutaminase-2) (TGase-2) PROTEIN FAMILY: Transglutaminase superfamily, Transglutaminase family ORGANISM: Cavia cutleri (Guinea pig) SIMILARITY: Belongs to the transglutaminase superfamily. Transglutaminase family. FUNCTION: Calcium-dependent acyltransferase that catalyzes the formation of covalent bonds between peptide-bound glutamine and various primary amines, such as gamma-amino group of peptide-bound lysine, or mono- and polyamines, thereby producing cross-linked or aminated proteins, respectively. Involved in many biological processes, such as bone development, angiogenesis, wound healing, cellular differentiation, chromatin modification and apoptosis (By similarity). Acts as a protein-glutamine gamma-glutamyltransferase by mediating the cross-linking of proteins, such as ACO2, HSPB6, FN1, HMGB1, RAP1GDS1, SLC25A4/ANT1, SPP1 and WDR54. Under physiological conditions, the protein cross-linking activity is inhibited by GTP; inhibition is relieved by Ca(2+) in response to various stresses (By similarity). When secreted, catalyzes cross-linking of proteins of the extracellular matrix, such as FN1 and SPP1 resulting in the formation of scaffolds (By similarity). Plays a key role during apoptosis, both by (1) promoting the cross-linking of cytoskeletal proteins resulting in condensation of the cytoplasm, and by (2) mediating cross-linking proteins of the extracellular matrix, resulting in the irreversible formation of scaffolds that stabilize the integrity of the dying cells before their clearance by phagocytosis, thereby preventing the leakage of harmful intracellular components (By similarity). In addition to protein cross-linking, can use different monoamine substrates to catalyze a vast array of protein post-translational modifications: mediates aminylation of serotonin, dopamine, noradrenaline or histamine into glutamine residues of target proteins to generate protein serotonylation, dopaminylation, noradrenalinylation or histaminylation, respectively. Mediates protein serotonylation of small GTPases during activation and aggregation of platelets, leading to constitutive activation of these GTPases. Plays a key role in chromatin organization by mediating serotonylation and dopaminylation of histone H3 (By similarity). Catalyzes serotonylation of 'Gln-5' of histone H3 (H3Q5ser) during serotonergic neuron differentiation, thereby facilitating transcription (By similarity). Acts as a mediator of neurotransmission-independent role of nuclear dopamine in ventral tegmental area (VTA) neurons: catalyzes dopaminylation of 'Gln-5' of histone H3 (H3Q5dop), thereby regulating relapse-related transcriptional plasticity in the reward system (By similarity). Regulates vein remodeling by mediating serotonylation and subsequent inactivation of ATP2A2/SERCA2 (By similarity). Also acts as a protein deamidase by mediating the side chain deamidation of specific glutamine residues of proteins to glutamate. Catalyzes specific deamidation of protein gliadin, a component of wheat gluten in the diet (Ref.6). May also act as an isopeptidase cleaving the previously formed cross-links (By similarity). Also able to participate in signaling pathways independently of its acyltransferase activity: acts as a signal transducer in alpha-1 adrenergic receptor-mediated stimulation of phospholipase C-delta (PLCD) activity and is required for coupling alpha-1 adrenergic agonists to the stimulation of phosphoinositide lipid metabolism (By similarity). KEYWORDS: Acetylation;Acyltransferase;Calcium;Cell membrane;Chromosome;Cytoplasm;Direct protein sequencing;Disulfide bond;Extracellular matrix;GTP-binding;Hydrolase;Isopeptide bond;Membrane;Metal-binding;Mitochondrion;Nucleotide-binding;Nucleus;Protease;S-nitrosylation;Secreted;Transferase SUBCELLULAR LOCATION: Cytoplasm, cytosol Nucleus Chromosome Secreted, extracellular space, extracellular matrix Cell membrane Mitochondrion Note=Mainly localizes to the cytosol. Present at much lower level in the nucleus and chromatin. Also secreted via a non-classical secretion pathway to the extracellular matrix. " P08630,"PROTEIN NAMES: Tyrosine-protein kinase Btk (Dsrc28C) (Dsrc29a) PROTEIN FAMILY: Protein kinase superfamily, Tyr protein kinase family, TEC subfamily ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily. FUNCTION: Required for proper ring canal development. Also required for the development of male genitalia and for adult survival. KEYWORDS: Alternative splicing;ATP-binding;Kinase;Metal-binding;Nucleotide-binding;Phosphoprotein;Proto-oncogene;Reference proteome;SH2 domain;SH3 domain;Transferase;Tyrosine-protein kinase;Zinc;Zinc-finger " P08638,PROTEIN NAMES: Regulatory protein LEU3 ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Factor for control of RNA levels of a group of leucine-specific genes. KEYWORDS: 3D-structure;Activator;Amino-acid biosynthesis;Branched-chain amino acid biosynthesis;DNA-binding;Leucine biosynthesis;Metal-binding;Nucleus;Reference proteome;Transcription;Transcription regulation;Zinc SUBCELLULAR LOCATION: Nucleus. MISCELLANEOUS: Present with 125 molecules/cell in log phase SD medium. P08709,"PROTEIN NAMES: Coagulation factor VII (Proconvertin) (Serum prothrombin conversion accelerator) (SPCA) (Eptacog alfa) [Cleaved into: Factor VII light chain; Factor VII heavy chain] PROTEIN FAMILY: Peptidase S1 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the peptidase S1 family. FUNCTION: Initiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. Factor VII is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, factor VIIa then converts factor X to factor Xa by limited proteolysis. Factor VIIa will also convert factor IX to factor IXa in the presence of tissue factor and calcium. KEYWORDS: 3D-structure;Alternative splicing;Blood coagulation;Calcium;Cleavage on pair of basic residues;Direct protein sequencing;Disease variant;Disulfide bond;EGF-like domain;Gamma-carboxyglutamic acid;Glycoprotein;Hemostasis;Hydrolase;Hydroxylation;Pharmaceutical;Protease;Reference proteome;Repeat;Secreted;Serine protease;Signal;Zymogen SUBCELLULAR LOCATION: Secreted. " P08897,"PROTEIN NAMES: Collagenase (Hypodermin C) (HC) PROTEIN FAMILY: Peptidase S1 family ORGANISM: Hypoderma lineatum (Early cattle grub) (Common cattle grub) SIMILARITY: Belongs to the peptidase S1 family. FUNCTION: This enzyme is a serine protease capable of degrading the native triple helix of collagen. Also cleaves the B chain of insulin at the 15-Leu-|-Try-16 and 22-Arg-|-Gly-23 bonds. Hydrolyzes casein, but not Px-Pro-Leu-Gly-Pro-DArg, BzArgNHPh, AcTyrNHPh, 2-naphthyl phosphate, 2-naphthyl butyrate, 2-naphthyl caprylate, 2-naphthyl myristate, L-leucine 2-2-naphthylamide, L-valine 2-naphthylamide, L-cysteine 2-naphthylamide or L-glutarylphenylalanine 2-naphthylamide. KEYWORDS: 3D-structure;Collagen degradation;Direct protein sequencing;Disulfide bond;Hydrolase;Protease;Secreted;Serine protease;Signal;Zymogen SUBCELLULAR LOCATION: Secreted. " P08970,"PROTEIN NAMES: Protein suppressor of hairy wing ORGANISM: Drosophila melanogaster (Fruit fly) FUNCTION: Component of the gypsy chromatin insulator complex which is required for the function of the gypsy chromatin insulator and other endogenous chromatin insulators. Chromatin insulators are regulatory elements which establish independent domains of transcriptional activity within eukaryotic genomes. Insulators have two defining properties; they can block the communication between an enhancer and a promoter when placed between them and can also buffer transgenes from position effect variegation (PEV). Insulators are proposed to structure the chromatin fiber into independent domains of differing transcriptional potential by promoting the formation of distinct chromatin loops. This chromatin looping may involve the formation of insulator bodies, where homotypic interactions between individual subunits of the insulator complex could promote the clustering of widely spaced insulators at the nuclear periphery. Within the gypsy insulator complex, this protein binds specifically to a region of the gypsy element located 3' of the 5' long terminal repeat (LTR), and may also mediate interaction with other endogenous insulators at sites distinct from those recognized by Cp190. KEYWORDS: Chromatin regulator;Chromosome;DNA-binding;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Repeat;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus. Chromosome. Note=Colocalizes with other elements of the gypsy chromatin insulator complex at multiple sites on polytene chromosomes and at nuclear insulator bodies. " P09030,"PROTEIN NAMES: Exodeoxyribonuclease III (EXO III) (Exonuclease III) (AP endonuclease VI) PROTEIN FAMILY: DNA repair enzymes AP/ExoA family ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the DNA repair enzymes AP/ExoA family. FUNCTION: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase and ribonuclease H activities. KEYWORDS: 3D-structure;DNA damage;DNA repair;Exonuclease;Hydrolase;Magnesium;Metal-binding;Nuclease;Reference proteome " P09077,"PROTEIN NAMES: Homeotic protein Sex combs reduced PROTEIN FAMILY: Antp homeobox family, Deformed subfamily ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the Antp homeobox family. Deformed subfamily. FUNCTION: Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Controls the segmental transformation of the first to the second thoracic segment (prothorax to mesothorax) and of the labial palps into maxillary palps. In embryo, required for fusion of labial lobes and development of the T1 denticle belt. In adult, expression in the head is necessary for proper development of the labium. In the first thoracic segment of the adult, required for proper development of the sex comb and to suppress improper prothoracic wing development. KEYWORDS: 3D-structure;Developmental protein;DNA-binding;Homeobox;Nucleus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Nucleus. " P09081,"PROTEIN NAMES: Homeotic protein bicoid (PRD-4) PROTEIN FAMILY: Paired homeobox family, Bicoid subfamily ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the paired homeobox family. Bicoid subfamily. FUNCTION: Segment polarity transcription factor that provides positional cues for the development of head and thoracic segments. Forms a protein concentration gradient that patterns the anterior-posterior axis during embryogenesis and promotes the expression of anterior gap genes, such as hunchback (hb), ocelliless (oc), and buttonhead (btd). Binds to regulatory DNA sequences containing a 5'-TAATCC-3' sequence motif. Also binds RNA. Interacts with Bin1 to repress transcription of bicoid target genes in the anterior tip of the embryo; a process known as retraction. KEYWORDS: 3D-structure;Alternative splicing;Developmental protein;DNA-binding;Homeobox;Nucleus;Reference proteome;RNA-binding;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus. " P09083,PROTEIN NAMES: Protein gooseberry-neuro (BSH4) (Protein gooseberry proximal) PROTEIN FAMILY: Paired homeobox family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the paired homeobox family. FUNCTION: Expressed in a segmentally repeating pattern to define the polarity of embryonic segments. KEYWORDS: Developmental protein;DNA-binding;Homeobox;Nucleus;Paired box;Phosphoprotein;Reference proteome;Segmentation polarity protein;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus. P09085,"PROTEIN NAMES: Homeotic protein caudal PROTEIN FAMILY: Caudal homeobox family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the Caudal homeobox family. FUNCTION: Caudal (cad) is one of a number of transcription factors controlling segmentation of the embryo. Further transcriptional regulation via a 5' flanking region containing DNA replication-related elements (DRE) and by dref also regulated by trh and tgo via the CNS midline element. Alongside Bicoid (bcd), caudal forms concentration gradients down the anterior-posterior (A-P) axis providing positional information and subsequent induction of the gap genes. Plays a role in gastrulation/germ band extension, hindgut morphogenesis, positive regulation of cell proliferation, genital disk development and pattern formation. Acts as a key regulator of the Hox gene network and activates transcription via the downstream core promoter element (DPE) relative to the TATA box. Plays a role in the establishment of the hindgut and in the invagination of the hindgut primordium during gastrulation. These effects on the gut are achieved by acting combinatorially at the posterior of the embryo to activate transcription of different targets including fog, fkh and wg. Caudal is involved in regulation of proliferation through transactivation of the E2F gene. Postembryonically its function is mostly restricted to the intestine where it regulates antimicrobial peptide (AMP) levels preserving the normal gut flora. KEYWORDS: Alternative splicing;Developmental protein;DNA-binding;Gastrulation;Homeobox;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " P09169,"PROTEIN NAMES: Protease 7 (Omptin) (Outer membrane protein 3B) (Protease A) (Protease VII) PROTEIN FAMILY: Peptidase A26 family ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the peptidase A26 family. FUNCTION: Protease that can cleave T7 RNA polymerase, ferric enterobactin receptor protein (FEP), antimicrobial peptide protamine and other proteins. This protease has a specificity for paired basic residues. KEYWORDS: 3D-structure;Aspartyl protease;Cell outer membrane;Direct protein sequencing;Hydrolase;Membrane;Protease;Reference proteome;Signal;Transmembrane;Transmembrane beta strand SUBCELLULAR LOCATION: Cell outer membrane; Multi-pass membrane protein. MISCELLANEOUS: Was originally classified as a serine protease, but it seems that OmpT could have a novel catalytic mechanism involving an Asp/His dyad and a pair of Asp.; MISCELLANEOUS: Encoded by the cryptic lambdoid prophage DLP12." P09230,PROTEIN NAMES: Alkaline extracellular protease (AEP) PROTEIN FAMILY: Peptidase S8 family ORGANISM: Yarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica) SIMILARITY: Belongs to the peptidase S8 family. FUNCTION: Major secreted protein that belongs to the subtilisin family serine proteases. KEYWORDS: Cleavage on pair of basic residues;Direct protein sequencing;Glycoprotein;Hydrolase;Protease;Reference proteome;Secreted;Serine protease;Signal;Zymogen SUBCELLULAR LOCATION: Secreted Note=Proper secretion requires TSR1. MISCELLANEOUS: The pro-region inhibits protease activity and plays an additional essential role in the proper folding of the protein into a conformation compatible with secretion.; MISCELLANEOUS: Its complex processing and high level of secretion make XPR2 the perfect model to study the secretion pathway. P09232,"PROTEIN NAMES: Cerevisin (Proteinase YSCB) (Vacuolar protease B) (PrB) PROTEIN FAMILY: Peptidase S8 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the peptidase S8 family. FUNCTION: Vacuolar proteinase B involved in protein degradation in the vacuole. Among other substrates, acts on carboxypeptidase Y (cpY/PRC1) to activate it by processing its Pro-peptide. Required for meiosis and spore formation, and for optimal survival in stationary phase. KEYWORDS: Amyloid;Direct protein sequencing;Disulfide bond;Glycoprotein;Hydrolase;Prion;Protease;Reference proteome;Serine protease;Signal;Vacuole;Zymogen SUBCELLULAR LOCATION: Vacuole. MISCELLANEOUS: [beta] is the prion form of PrB. In contrast to other prions, [beta] is not the result of a conformational change of the cellular PrB, but distinguishes itself by autoactivation in trans. Usually, PrB is already involved in its own maturatiuon, but PrA plays a critical role. PrpA mutants lack PrB. However, in growth conditions that favor PRB1 expression, PrB activity persists in PrA mutants due to autocleavage of PrB in trans. This condition is stably transmitted to daughter cells in mitosis. [beta] can be cured by growing in PRB1-repressing conditions. Once a cell has lost PrB activity, it remains stably inactive. Thus, there are 2 alternative states, that are chromosomally identical, but phenotypically distinct. Since PrA is able to activate PrB, normal cells always carry the [beta] prion. Its absence and transmission are only observable in the absence of PrA.; MISCELLANEOUS: Present with 1600 molecules/cell in log phase SD medium." P09348,"PROTEIN NAMES: Motility protein A (Chemotaxis protein MotA) PROTEIN FAMILY: MotA family ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the MotA family. FUNCTION: MotA and MotB comprise the stator element of the flagellar motor complex. Required for rotation of the flagellar motor. Probable transmembrane proton channel. Overexpression of MotA, with or without MotB, restores motility in a pdeH disruption, (a c-di-GMP phosphodiesterase) suggesting there is an interaction (direct or indirect) between the c-di-GMP-binding flagellar brake protein YcgR and the flagellar stator. KEYWORDS: Cell inner membrane;Cell membrane;Chemotaxis;Flagellar rotation;Hydrogen ion transport;Ion transport;Membrane;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein. " P09440,"PROTEIN NAMES: C-1-tetrahydrofolate synthase, mitochondrial (C1-THF synthase) [Includes: Methylenetetrahydrofolate dehydrogenase ; Methenyltetrahydrofolate cyclohydrolase ; Formyltetrahydrofolate synthetase ] PROTEIN FAMILY: Tetrahydrofolate dehydrogenase/cyclohydrolase family; Formate--tetrahydrofolate ligase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: In the N-terminal section; belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.; SIMILARITY: In the C-terminal section; belongs to the formate--tetrahydrofolate ligase family. FUNCTION: Mitochondrial isozyme of C-1-tetrahydrofolate synthase. The trifunctional enzyme catalyzes the interconversion of the one-carbon derivatives of tetrahydrofolate (THF) between different oxidation states by the enzymatic activities 10-formyltetrahydrofolate synthetase, 5,lO-methenyltetrahydrofolate cyclohydrolase, and 5,lO-methylenetetrahydrofolate dehydrogenase. PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion. KEYWORDS: ATP-binding;Direct protein sequencing;Hydrolase;Ligase;Mitochondrion;Multifunctional enzyme;NADP;Nucleotide-binding;One-carbon metabolism;Oxidoreductase;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Mitochondrion MISCELLANEOUS: Present with 11400 molecules/cell in log phase SD medium." P09483,"PROTEIN NAMES: Neuronal acetylcholine receptor subunit alpha-4 PROTEIN FAMILY: Ligand-gated ion channel family, Acetylcholine receptor subfamily, Alpha-4/CHRNA4 sub-subfamily ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-4/CHRNA4 sub-subfamily. FUNCTION: After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane permeable to sodium ions. KEYWORDS: Alternative splicing;Cell membrane;Direct protein sequencing;Disulfide bond;Glycoprotein;Ion channel;Ion transport;Ligand-gated ion channel;Lipoprotein;Membrane;Palmitate;Phosphoprotein;Postsynaptic cell membrane;Receptor;Reference proteome;Signal;Synapse;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Postsynaptic cell membrane ; Multi-pass membrane protein Cell membrane ; Multi-pass membrane protein Cell membrane ; Lipid-anchor MISCELLANEOUS: The nAChR composed of alpha-4 and beta-2 subunits does not bind the conotoxin BuIA." P09543,"PROTEIN NAMES: 2',3'-cyclic-nucleotide 3'-phosphodiesterase (CNP) (CNPase) PROTEIN FAMILY: 2H phosphoesterase superfamily, CNPase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the 2H phosphoesterase superfamily. CNPase family. FUNCTION: Catalyzes the formation of 2'-nucleotide products from 2',3'-cyclic substrates (By similarity). May participate in RNA metabolism in the myelinating cell, CNP is the third most abundant protein in central nervous system myelin (By similarity). KEYWORDS: 3D-structure;Alternative splicing;Direct protein sequencing;Hydrolase;Leukodystrophy;Lipoprotein;Membrane;Methylation;Neurodegeneration;Phosphoprotein;Prenylation;Reference proteome;RNA-binding SUBCELLULAR LOCATION: Membrane ; Lipid-anchor Melanosome Note=Firmly bound to membrane structures of brain white matter. " P09615,"PROTEIN NAMES: Protein wingless (Protein Wnt-1) (Protein int-1) (dInt-1) (dWnt-1) PROTEIN FAMILY: Wnt family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the Wnt family. FUNCTION: Binds as a ligand to a family of frizzled seven-transmembrane receptors and acts through a cascade of genes on the nucleus. Segment polarity protein. May be a growth factor. Acts on neighboring cells to regulate at least one gene, the homeobox segmentation gene engrailed. Wg signal represses arm phosphorylation. Wg signaling operates by inactivating the sgg repression of engrailed autoactivation. Wg and Wnt2 have a role in the developing trachea and together are responsible for all dorsal trunk formation. Wg also acts in the developing epidermis. Acts as a morphogen, and diffuses long distances despite its lipidation. Lipophorin is required for diffusion, probably by acting as vehicle for its movement, explaining how it can spread over long distances despite its lipidation. In non-neuronal cells, wls directs wg secretion via clathrin-mediated endocytosis and the retromer complex (a conserved protein complex consisting of Vps26 and Vps35) to sustain a wls traffic loop encompassing the Golgi, the cell surface, an endocytic compartment and a retrograde route leading back to the Golgi. In neuronal cells (the larval motorneuron NMJ), wg signal moves across the synapse through the release of wls-containing exosome-like vesicles. KEYWORDS: Alternative splicing;Developmental protein;Disulfide bond;Extracellular matrix;Glycoprotein;Lipoprotein;Membrane;Morphogen;Reference proteome;Secreted;Segmentation polarity protein;Signal;Synapse;Wnt signaling pathway SUBCELLULAR LOCATION: Secreted Synapse Membrane; Lipid-anchor. Secreted, extracellular space, extracellular matrix Note=Palmitoleoylation converts wg into a membrane-anchored protein that is partitioned into specialized lipid raft microdomains before secretion. In the wing imaginal disK epithelium, the protein is produced in the apical region of the cell and undergoes transcytosis to the basolateral surface to be released in the extracellular space. Possibly associated with the extracellular matrix. " P09758,PROTEIN NAMES: Tumor-associated calcium signal transducer 2 (Cell surface glycoprotein Trop-2) (Membrane component chromosome 1 surface marker 1) (Pancreatic carcinoma marker protein GA733-1) PROTEIN FAMILY: EPCAM family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the EPCAM family. FUNCTION: May function as a growth factor receptor. KEYWORDS: 3D-structure;Amyloid;Amyloidosis;Corneal dystrophy;Disulfide bond;Glycoprotein;Membrane;Reference proteome;Sensory transduction;Signal;Transmembrane;Transmembrane helix;Tumor antigen;Vision SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. P09785,"PROTEIN NAMES: Anthranilate synthase component 1, pyocyanine specific (AS) (ASI) (Anthranilate synthase alpha subunit, pyocyanine specific) PROTEIN FAMILY: Anthranilate synthase component I family ORGANISM: Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) SIMILARITY: Belongs to the anthranilate synthase component I family. FUNCTION: Part of a heterotetrameric complex that catalyzes the two-step biosynthesis of anthranilate, a precursor for Pseudomonas quinolone signal (2-heptyl-3-hydroxy-4-quinolone; PQS) production which is required to induce the genes for the biosynthesis of the virulence factor pyocyanine (PCN), a characteristic blue-green phenazine pigment produced by P.aeruginosa. In the first step, the glutamine-binding beta subunit (PhnB) of anthranilate synthase (AS) provides the glutamine amidotransferase activity which generates ammonia as a substrate that, along with chorismate, is used in the second step, catalyzed by the large alpha subunit of AS (PhnA) to produce anthranilate (By similarity). PATHWAY: Secondary metabolite biosynthesis; pyocyanine biosynthesis. KEYWORDS: Lyase;Magnesium;Metal-binding;Reference proteome;Virulence MISCELLANEOUS: PhnAB is not feedback inhibited by tryptophan.; MISCELLANEOUS: The sequence shown is that of strain PAO." P09959,PROTEIN NAMES: Regulatory protein SWI6 (Cell-cycle box factor subunit SWI6) (MBF subunit P90) (Trans-acting activator of HO endonuclease gene) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Part of a complex involved in cell-cycle-dependent transcription. SWI4 and SWI6 are required for formation of the cell-cycle box factor-DNA complex. The repeated element in the upstream region of HO (5'-CACGAAAA-3') is called the cell cycle box (CCB). KEYWORDS: 3D-structure;Activator;ANK repeat;Cytoplasm;DNA-binding;Nucleus;Phosphoprotein;Reference proteome;Repeat;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus. Cytoplasm. MISCELLANEOUS: Present with 3340 molecules/cell in log phase SD medium. P0A2M9,"PROTEIN NAMES: MAPK phosphothreonine lyase (27.5 kDa virulence protein) (Secreted effector protein SpvC) PROTEIN FAMILY: Phosphothreonine lyase family ORGANISM: Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) SIMILARITY: Belongs to the phosphothreonine lyase family. FUNCTION: Secreted effector that irreversibly inactivates host MAP kinases by catalyzing the dephosphorylation of the phosphothreonine residue in the pT-X-pY motif in MAPK2/ERK2, MAPK3/ERK1, and p38, via a beta-elimination reaction leading to a dehydrobutyrine residue. Is also able to remove the phosphate group from phospho-JNK in vitro, but JNK may not be a substrate in vivo. Could help suppress localized pro-inflammatory responses at infection foci in the spleen and liver, and thereby facilitate bacterial growth. KEYWORDS: 3D-structure;Direct protein sequencing;Lyase;Plasmid;Reference proteome;Secreted;Virulence SUBCELLULAR LOCATION: Secreted Note=Can be secreted in vitro by either the SPI-1 or SPI-2 type III secretion systems (T3SS). Translocation of the protein into the cytosol of infected macrophages by intracellular bacteria is dependent on the SPI-2 T3SS. Translocated SpvC proteins appear to be distributed evenly in the cytoplasm of infected cells, and neither colocalize with the SCV membrane nor accumulate in the nucleus of infected cells. " P0A991,"PROTEIN NAMES: Fructose-bisphosphate aldolase class 1 (Fructose-bisphosphate aldolase class I) (FBP aldolase) PROTEIN FAMILY: DeoC/FbaB aldolase family, FbaB subfamily ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the DeoC/FbaB aldolase family. FbaB subfamily. FUNCTION: Catalyzes the reversible aldol condensation/cleavage reaction between glyceraldehyde 3-phosphate and dihydroxyacetone phosphate (DHAP) to yield fructose-bisphosphate (FBP). KEYWORDS: Acetylation;Cytoplasm;Direct protein sequencing;Glycolysis;Lyase;Reference proteome;Schiff base SUBCELLULAR LOCATION: Cytoplasm " P0A9Q1,"PROTEIN NAMES: Aerobic respiration control protein ArcA (Dye resistance protein) ORGANISM: Escherichia coli (strain K12) FUNCTION: Member of the two-component regulatory system ArcB/ArcA. Represses a wide variety of aerobic enzymes under anaerobic conditions. Controls the resistance of E.coli to dyes; required for expression of the alkaline phosphatase and sex factor F genes; it may also be involved in the osmoregulation of envelope proteins. When activated by ArcB, it negatively regulates the expression of genes of aerobic function. Activates the transcription of the plfB operon by binding to its promoter. KEYWORDS: 3D-structure;Activator;Cytoplasm;Direct protein sequencing;DNA-binding;Phosphoprotein;Reference proteome;Repressor;Transcription;Transcription regulation;Two-component regulatory system SUBCELLULAR LOCATION: Cytoplasm " P0A9T0,"PROTEIN NAMES: D-3-phosphoglycerate dehydrogenase (PGDH) (2-oxoglutarate reductase) PROTEIN FAMILY: D-isomer specific 2-hydroxyacid dehydrogenase family ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. FUNCTION: Catalyzes the reversible oxidation of 3-phospho-D-glycerate to 3-phosphonooxypyruvate, the first step of the phosphorylated L-serine biosynthesis pathway. Also catalyzes the reversible oxidation of 2-hydroxyglutarate to 2-oxoglutarate. PATHWAY: Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 1/3. KEYWORDS: 3D-structure;Amino-acid biosynthesis;Direct protein sequencing;NAD;Oxidoreductase;Reference proteome;Serine biosynthesis " P0A9V8,"PROTEIN NAMES: 3-sulfolactaldehyde reductase (SLA reductase) (4-hydroxybutyrate dehydrogenase) (Gamma-hydroxybutyrate dehydrogenase) (GHBDH) (Succinic semialdehyde reductase) (SSA reductase) PROTEIN FAMILY: HIBADH-related family, 3-sulfolactaldehyde reductase subfamily ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the HIBADH-related family. 3-sulfolactaldehyde reductase subfamily. FUNCTION: Reduces 3-sulfolactaldehyde (SLA) to 2,3-dihydroxypropane 1-sulfonate (DHPS). Metabolite profiling studies showed that the enzyme also catalyzes in vitro the NADH-dependent reduction of succinic semialdehyde (SSA) to 4-hydroxybutyrate (GHB), and that it could be involved in the metabolism of SSA, and other potentially toxic intermediates that may accumulate under stress conditions. However, the enzyme exhibits a 42,000-fold greater catalytic efficiency for the reduction of SLA over SSA (Ref.6). Shows no detectable activity on the analogous glycolytic intermediate glyceraldehyde-3-phosphate (Ref.6). KEYWORDS: 3D-structure;NAD;Oxidoreductase;Reference proteome " P0AA76,"PROTEIN NAMES: D-galactonate transporter (D-galactonate/H(+) symporter) PROTEIN FAMILY: Major facilitator superfamily, Phthalate permease family ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the major facilitator superfamily. Phthalate permease family. FUNCTION: Involved in D-galactonate metabolism. Catalyzes the proton-dependent uptake of galactonate into the cell. KEYWORDS: 3D-structure;Cell inner membrane;Cell membrane;Membrane;Reference proteome;Symport;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell inner membrane ; Multi-pass membrane protein MISCELLANEOUS: The exact stoichiometry of cotransport is not determined and could be greater than 1." P0AA84,"PROTEIN NAMES: Cardiolipin synthase B (CL synthase) PROTEIN FAMILY: Phospholipase D family, Cardiolipin synthase subfamily, ClsB sub-subfamily ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the phospholipase D family. Cardiolipin synthase subfamily. ClsB sub-subfamily. FUNCTION: Catalyzes the phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol. Can also catalyze phosphatidyl group transfer to water to form phosphatidate. KEYWORDS: Cell membrane;Lipid biosynthesis;Lipid metabolism;Membrane;Phospholipid biosynthesis;Phospholipid metabolism;Reference proteome;Repeat;Transferase SUBCELLULAR LOCATION: Cell membrane ; Peripheral membrane protein MISCELLANEOUS: All three cardiolipin synthases (ClsA, ClsB and ClsC) contribute to CL synthesis in stationary phase. Only ClsA contributes to synthesis during logarithmic growth phase." P0AA93,"PROTEIN NAMES: Sensor histidine kinase YpdA ORGANISM: Escherichia coli (strain K12) FUNCTION: Member of the two-component regulatory system YpdA/YpdB, which is part of a nutrient-sensing regulatory network composed of YpdA/YpdB, the high-affinity pyruvate signaling system BtsS/BtsR and their respective target proteins, YhjX and BtsT. YpdA activates YpdB by phosphorylation in response to high concentrations of extracellular pyruvate. Activation of the YpdA/YpdB signaling cascade also promotes BtsS/BtsR-mediated btsT expression. KEYWORDS: ATP-binding;Cell inner membrane;Cell membrane;Kinase;Membrane;Nucleotide-binding;Phosphoprotein;Reference proteome;Transferase;Transmembrane;Transmembrane helix;Two-component regulatory system SUBCELLULAR LOCATION: Cell inner membrane ; Multi-pass membrane protein " P0AAE8,"PROTEIN NAMES: Cadaverine/lysine antiporter PROTEIN FAMILY: Amino acid-polyamine-organocation (APC) superfamily, Basic amino acid/polyamine antiporter (APA) family ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the amino acid-polyamine-organocation (APC) superfamily. Basic amino acid/polyamine antiporter (APA) (TC 2.A.3.2) family. FUNCTION: Under acidic conditions, in the presence of lysine, functions as a cadaverine:lysine antiporter that facilitates the excretion of cadaverine and the uptake of lysine. At neutral pH, also catalyzes the uptake of cadaverine via a proton symport mechanism, however the physiological relevance of this uptake activity is probably negligible because the expression of cadB is low at neutral pH. Cadaverine uptake activity is low at acidic pH. KEYWORDS: Amino-acid transport;Antiport;Cell inner membrane;Cell membrane;Membrane;Reference proteome;Symport;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell inner membrane ; Multi-pass membrane protein " P0AAM1,PROTEIN NAMES: Probable Ni/Fe-hydrogenase 1 B-type cytochrome subunit PROTEIN FAMILY: HupC/HyaC/HydC family ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the HupC/HyaC/HydC family. FUNCTION: Probable b-type cytochrome. KEYWORDS: 3D-structure;Cell inner membrane;Cell membrane;Electron transport;Heme;Iron;Membrane;Metal-binding;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein. P0ABP3,"PROTEIN NAMES: Anaerobic C4-dicarboxylate transporter DcuC PROTEIN FAMILY: DcuC/DcuD transporter family ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the DcuC/DcuD transporter (TC 2.A.61) family. FUNCTION: Responsible for the transport of C4-dicarboxylates during anaerobic growth. Catalyzes the uptake of fumarate coupled to the export of succinate. Can also catalyze the uptake of fumarate and the efflux of succinate, without exchange. Shows low rates of transport, which are sufficient for succinate export during fermentation but not for fumarate-succinate exchange in fumarate respiration, indicating that it may function in vivo as the succinate efflux carrier for glucose fermentation, even if it is also able to operate as a fumarate-succinate antiporter. KEYWORDS: Antiport;Cell inner membrane;Cell membrane;Membrane;Reference proteome;Symport;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell inner membrane ; Multi-pass membrane protein " P0AC00,PROTEIN NAMES: Fructoselysine 6-phosphate deglycase ORGANISM: Escherichia coli (strain K12) FUNCTION: Catalyzes the reversible conversion of fructoselysine 6-phosphate to glucose 6-phosphate and lysine. Functions in a fructoselysine degradation pathway that allows E.coli to grow on fructoselysine or psicoselysine. PATHWAY: Carbohydrate metabolism; fructoselysine degradation; D-glucose 6-phosphate and lysine from fructoselysine: step 2/2. KEYWORDS: Hydrolase;Reference proteome;Repeat P0AC30,"PROTEIN NAMES: Cell division protein FtsX PROTEIN FAMILY: ABC-4 integral membrane protein family, FtsX subfamily ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the ABC-4 integral membrane protein family. FtsX subfamily. FUNCTION: Part of the ABC transporter FtsEX involved in cellular division. Important for assembly or stability of the septal ring. Encoded in an operon consisting of genes ftsY, ftsE and ftsX. KEYWORDS: 3D-structure;Cell cycle;Cell division;Cell inner membrane;Cell membrane;Membrane;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell inner membrane ; Multi-pass membrane protein Note=Localizes to the septal ring at the later stages of cell growth and remains there until division is complete. This localization is dependent on localization of FtsZ, FtsA and ZipA, but not on the downstream division proteins FtsK, FtsQ or FtsI. MISCELLANEOUS: Overexpression leads to strong inhibition of growth, cell filamentation and eventually cell death." P0ACB4,"PROTEIN NAMES: Protoporphyrinogen IX dehydrogenase [quinone] (Protoporphyrinogen IX dehydrogenase [menaquinone]) (Protoporphyrinogen IX dehydrogenase [ubiquinone]) (Protoporphyrinogen oxidase) (PPO) PROTEIN FAMILY: HemG family ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the HemG family. FUNCTION: Catalyzes the 6-electron oxidation of protoporphyrinogen IX to form protoporphyrin IX; under anaerobic conditions uses menaquinone as an electron acceptor, under aerobic condition uses ubiquinone as an electron acceptor.; FUNCTION: Anaerobically in vitro transfers electrons to fumarate reductase and nitrate reductase; transfer to nitrate reductase couples this reaction to electron transfer across the cell inner membrane and thus ATP synthesis. Neither mesoporphyrinogen nor coproporphyrinogen are substrates. Under aerobic conditions in vitro forms protoporphyrin IX using ubiquinone as an electron acceptor, is able to transfer electrons to cytochrome bd oxidase and cytochrome bo oxidase; transfer to these oxidases couples this reaction to electron transfer across the cell inner membrane and thus ATP synthesis. In cell free extracts deletion of both cytochrome oxidases prevents formation of protoporphyrin IX. PATHWAY: Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; protoporphyrin-IX from protoporphyrinogen-IX: step 1/1. KEYWORDS: Cell inner membrane;Cell membrane;Direct protein sequencing;Flavoprotein;FMN;Membrane;Nucleotide-binding;Oxidoreductase;Porphyrin biosynthesis;Reference proteome SUBCELLULAR LOCATION: Cell inner membrane ; Peripheral membrane protein " P0ADA1,"PROTEIN NAMES: Thioesterase 1/protease 1/lysophospholipase L1 (TAP) (Acyl-CoA thioesterase 1) (TESA) (Acyl-CoA thioesterase I) (Arylesterase) (Lysophospholipase L1) (Oleoyl-[acyl-carrier-protein] hydrolase) (Phospholipid degradation C) (Pldc) (Protease 1) (Protease I) (Thioesterase I/protease I) (TEP-I) PROTEIN FAMILY: 'GDSL' lipolytic enzyme family ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the 'GDSL' lipolytic enzyme family. FUNCTION: TesA is a multifunctional esterase that can act as a thioesterase, lysophospholipase and protease. TesA functions as a thioesterase specific for fatty acyl thioesters of greater than ten carbons, with highest activity on palmitoyl-CoA, cis-vaccenoyl-CoA and palmitoleoyl-CoA. TesA also possesses an arylesterase activity towards short acyl-chain aromatic esters such as alpha-naphthyl acetate, alpha-naphthyl butyrate, benzyl acetate and phenyl acetate. Also able to hydrolyze short acyl-chain triacylglycerols such as triacetin and tributyrin, and p-nitrophenyl esters such as p-nitrophenyl hexanoate and p-nitrophenyl butyrate. The protease activity is mainly active on small peptides. TesA is also able to hydrolyze p-nitrophenyl esters of N-substituted amino acids such as N-benzyloxycarbonyl-L-Phe-p-nitrophenyl ester (Z-L-Phe-ONp) and N-benzyloxycarbonyl-L-Tyr-p-nitrophenyl ester (Z-L-Tyr-ONp), however it is unable to hydrolyze N-acetyl-L-Phe ethyl ester and its Tyr analog. TesA also hydrolyzes N-benzyloxycarbonyl-L-Phe beta-nitrophenyl ester (Cbz-Phe-ONap) and N-acetyl-DL-Phe-2-naphthyl ester (chymotrypsin-like specificity). Shows a slow proteolytic activity against denatured casein. The lysophospholipase activity of TesA is able to hydrolyze 1-palmitoyl-sn-glycero-3-phosphocholine, 1-acyl-sn-glycero-3-phosphoglycerol, 1- and 2-acyl-sn-glycero-3-phosphoethanolamine. KEYWORDS: 3D-structure;Direct protein sequencing;Hydrolase;Lipid metabolism;Periplasm;Protease;Reference proteome;Signal SUBCELLULAR LOCATION: Periplasm " P0AE12,"PROTEIN NAMES: AMP nucleosidase PROTEIN FAMILY: AMP nucleosidase family ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the AMP nucleosidase family. FUNCTION: Catalyzes the hydrolysis of the N-glycosidic bond of AMP to form adenine and ribose 5-phosphate. Involved in regulation of AMP concentrations. KEYWORDS: 3D-structure;Allosteric enzyme;Direct protein sequencing;Hydrolase;Reference proteome MISCELLANEOUS: AMP nucleosidase binds AMP at the catalytic site, Mg-ATP at an allosteric regulatory site, and inorganic phosphate also at a regulatory site." P0AEC8,"PROTEIN NAMES: Sensor histidine kinase DcuS (Fumarate sensor) ORGANISM: Escherichia coli (strain K12) FUNCTION: Member of the two-component regulatory system DcuR/DcuS. Involved in the C4-dicarboxylate-stimulated regulation of the genes encoding the anaerobic fumarate respiratory system (frdABCD; nuoAN; dcuB; sdhCDAB; etc.). Weakly regulates the aerobic C4-dicarboxylate transporter dctA. Activates DcuR by phosphorylation. KEYWORDS: 3D-structure;ATP-binding;Cell inner membrane;Cell membrane;Kinase;Membrane;Nucleotide-binding;Phosphoprotein;Reference proteome;Transferase;Transmembrane;Transmembrane helix;Two-component regulatory system SUBCELLULAR LOCATION: Cell inner membrane ; Multi-pass membrane protein MISCELLANEOUS: The region encompassing approximately residues 42 to 181 has been shown to be periplasmic, however exactly which residues are periplasmic is not clear." P0AEJ2,"PROTEIN NAMES: Isochorismate synthase EntC (Isochorismate mutase) PROTEIN FAMILY: Isochorismate synthase family ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the isochorismate synthase family. FUNCTION: Involved in the biosynthesis of the siderophore enterobactin (macrocyclic trimeric lactone of N-(2,3-dihydroxybenzoyl)-serine). Catalyzes the reversible conversion of chorismate to isochorismate. PATHWAY: Siderophore biosynthesis; enterobactin biosynthesis. KEYWORDS: 3D-structure;Direct protein sequencing;Enterobactin biosynthesis;Isomerase;Magnesium;Metal-binding;Reference proteome " P0AEM0,"PROTEIN NAMES: FKBP-type 16 kDa peptidyl-prolyl cis-trans isomerase (PPIase) (Rotamase) PROTEIN FAMILY: FKBP-type PPIase family ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the FKBP-type PPIase family. FUNCTION: PPIases accelerate the folding of proteins (Probable). Substrate specificity investigated with 'Suc-Ala-Xaa-Pro-Phe-4-nitroanilide' where Xaa is the amino acid tested, was found to be Phe > Leu >> Ile > Lys = Ala > Trp > His >> Gln. KEYWORDS: 3D-structure;Isomerase;Reference proteome;Rotamase " P0AEM9,"PROTEIN NAMES: L-cystine-binding protein TcyJ (CBP) (Protein FliY) (Sulfate starvation-induced protein 7) (SSI7) PROTEIN FAMILY: Bacterial solute-binding protein 3 family ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the bacterial solute-binding protein 3 family. FUNCTION: Part of the ABC transporter complex TcyJLN involved in L-cystine import. This high affinity cystine transporter is involved in resistance to oxidative stress by forming a L-cysteine/L-cystine shuttle system with the EamA transporter, which exports L-cysteine as reducing equivalents to the periplasm to prevent the cells from oxidative stress. Exported L-cysteine can reduce the periplasmic hydrogen peroxide to water, and then generated L-cystine is imported back into the cytoplasm via the TcyJLN complex. Functions at low cystine concentrations. The system can also transport L-cysteine, diaminopimelic acid (DAP), djenkolate, lanthionine, D-cystine, homocystine, and it mediates accumulation of the toxic compounds L-selenaproline (SCA) and L-selenocystine (SeCys). Binds cystine and DAP. KEYWORDS: Amino-acid transport;Direct protein sequencing;Periplasm;Reference proteome;Signal;Transport SUBCELLULAR LOCATION: Periplasm " P0AEP9,"PROTEIN NAMES: Glycolate oxidase subunit GlcD (Glycolate dehydrogenase subunit GlcD) PROTEIN FAMILY: FAD-binding oxidoreductase/transferase type 4 family ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the FAD-binding oxidoreductase/transferase type 4 family. FUNCTION: Component of a complex that catalyzes the oxidation of glycolate to glyoxylate. Is required for E.coli to grow on glycolate as a sole source of carbon. Is also able to oxidize D-lactate ((R)-lactate) with a similar rate. Does not link directly to O(2), and 2,6-dichloroindophenol (DCIP) and phenazine methosulfate (PMS) can act as artificial electron acceptors in vitro, but the physiological molecule that functions as a primary electron acceptor during glycolate oxidation is unknown. KEYWORDS: Cell inner membrane;Cell membrane;FAD;Flavoprotein;Membrane;Oxidoreductase;Reference proteome SUBCELLULAR LOCATION: Cell inner membrane Note=Glycolate oxidoreductase activity was shown to be firmly associated with the cytoplasmic membranes. " P0AEV1,"PROTEIN NAMES: Regulator of RpoS PROTEIN FAMILY: RssB family ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the RssB family. FUNCTION: Regulates the turnover of the sigma S factor (RpoS) by promoting its proteolysis in exponentially growing cells. Acts by binding and delivering RpoS to the ClpXP protease. RssB is not co-degraded with RpoS, but is released from the complex and can initiate a new cycle of RpoS recognition and degradation. In stationary phase, could also act as an anti-sigma factor and reduce the ability of RpoS to activate gene expression. Is also involved in the regulation of the mRNA polyadenylation pathway during stationary phase, probably by maintaining the association of PcnB with the degradosome. KEYWORDS: 3D-structure;Phosphoprotein;Reference proteome;Stress response " P0AEX9,PROTEIN NAMES: Maltose/maltodextrin-binding periplasmic protein (MMBP) (Maltodextrin-binding protein) (Maltose-binding protein) (MBP) PROTEIN FAMILY: Bacterial solute-binding protein 1 family ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the bacterial solute-binding protein 1 family. FUNCTION: Part of the ABC transporter complex MalEFGK involved in maltose/maltodextrin import. Binds maltose and higher maltodextrins such as maltotriose. KEYWORDS: 3D-structure;Direct protein sequencing;Periplasm;Reference proteome;Signal;Sugar transport;Transport SUBCELLULAR LOCATION: Periplasm P0AF24,"PROTEIN NAMES: Ribonucleotide monophosphatase NagD PROTEIN FAMILY: HAD-like hydrolase superfamily, NagD family ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the HAD-like hydrolase superfamily. NagD family. FUNCTION: Catalyzes the dephosphorylation of an unusually broad range of substrate including deoxyribo- and ribonucleoside tri-, di-, and monophosphates, as well as polyphosphate and glucose-1-P (Glu1P). KEYWORDS: 3D-structure;Carbohydrate metabolism;Hydrolase;Magnesium;Metal-binding;Reference proteome " P0AFC0,PROTEIN NAMES: Dihydroneopterin triphosphate diphosphatase (Dihydroneopterin triphosphate pyrophosphatase) (dATP pyrophosphohydrolase) PROTEIN FAMILY: Nudix hydrolase family ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the Nudix hydrolase family. FUNCTION: Catalyzes the hydrolysis of dihydroneopterin triphosphate to dihydroneopterin monophosphate and pyrophosphate. Required for efficient folate biosynthesis. Can also hydrolyze nucleoside triphosphates with a preference for dATP. KEYWORDS: 3D-structure;Folate biosynthesis;Hydrolase;Magnesium;Metal-binding;Reference proteome P0AFF4,"PROTEIN NAMES: Nucleoside permease NupG (Nucleoside-transport system protein NupG) PROTEIN FAMILY: Major facilitator superfamily, Nucleoside:H(+) symporter (NHS) family ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the major facilitator superfamily. Nucleoside:H(+) symporter (NHS) (TC 2.A.1.10) family. FUNCTION: Broad-specificity transporter of purine and pyrimidine nucleosides. Can transport adenosine, uridine, thymidine, cytidine, deoxycytidine, guanosine and inosine. Can also transport xanthosine, but with a very low affinity. Transport is driven by a proton motive force. KEYWORDS: 3D-structure;Cell inner membrane;Cell membrane;Direct protein sequencing;Membrane;Reference proteome;Symport;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell inner membrane ; Multi-pass membrane protein MISCELLANEOUS: Binding of nucleosides to NupG requires the presence of hydroxyl groups at each of the C-3' and C-5' positions of ribose." P0AFJ7,"PROTEIN NAMES: Low-affinity inorganic phosphate transporter PitA (Metal phosphate:H(+) symporter PitA) PROTEIN FAMILY: Inorganic phosphate transporter (PiT) family, Pit subfamily ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily. FUNCTION: Low-affinity inorganic phosphate transporter. Mediates proton-driven uptake of soluble neutral metal phosphate (MeHP04) complexes. It can use Mg(2+), Ca(2+), Co(2+) and Mn(2+). Activity impacts bacterial growth in low Mg(2+) conditions. Is also involved in Zn(2+) uptake, probably via formation of a ZnHPO4 complex. Can also transport arsenate. Involved in the uptake of tellurite. KEYWORDS: Cell inner membrane;Cell membrane;Ion transport;Magnesium;Membrane;Phosphate transport;Reference proteome;Symport;Transmembrane;Transmembrane helix;Transport;Zinc;Zinc transport SUBCELLULAR LOCATION: Cell inner membrane ; Multi-pass membrane protein MISCELLANEOUS: The Pit system in E.coli K12 consists of two transporters, PitA and PitB, which can transport phosphate independently of each other. Both proteins may have contributed to the kinetic values and substrate specificities determined in earlier studies." P0AFV4,PROTEIN NAMES: Murein DD-endopeptidase MepS/Murein LD-carboxypeptidase (Lipoprotein Spr) (Murein hydrolase MepS) PROTEIN FAMILY: Peptidase C40 family ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the peptidase C40 family. FUNCTION: A murein DD-endopeptidase with specificity for D-Ala-meso-diaminopimelic acid (mDAP) cross-links. Its role is probably to cleave D-Ala-mDAP cross-links to allow insertion of new glycans and thus cell wall expansion. Functionally redundant with MepM and MepH. Also has weak LD-carboxypeptidase activity on L-mDAP-D-Ala peptide bonds. Partially suppresses a prc disruption mutant. PATHWAY: Cell wall biogenesis; cell wall polysaccharide biosynthesis. KEYWORDS: 3D-structure;Cell outer membrane;Cell wall biogenesis/degradation;Hydrolase;Lipoprotein;Membrane;Palmitate;Protease;Reference proteome;Signal;Thiol protease SUBCELLULAR LOCATION: Cell outer membrane ; Lipid-anchor P0AG93,"PROTEIN NAMES: Protein translocase subunit SecF (Sec translocon accessory complex subunit SecF) PROTEIN FAMILY: SecD/SecF family, SecF subfamily ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the SecD/SecF family. SecF subfamily. FUNCTION: Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. SecDF uses the proton motive force (PMF) to complete protein translocation after the ATP-dependent function of SecA. The large periplasmic domain is thought to have a base and head domain joined by a hinge; movement of the hinge may be coupled to both proton transport and protein export, with the head domain capturing substrate, and a conformational change preventing backward movement and driving forward movement. Expression of V.alginolyticus SecD and SecF in E.coli confers Na(+)-dependent protein export, strongly suggesting SecDF functions via cation-coupled protein translocation. KEYWORDS: 3D-structure;Cell inner membrane;Cell membrane;Membrane;Protein transport;Reference proteome;Translocation;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein. " P0AGC0,"PROTEIN NAMES: Hexose-6-phosphate:phosphate antiporter PROTEIN FAMILY: Major facilitator superfamily, Organophosphate:Pi antiporter (OPA) family ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the major facilitator superfamily. Organophosphate:Pi antiporter (OPA) (TC 2.A.1.4) family. FUNCTION: Mediates the exchange of external hexose 6-phosphate and internal inorganic phosphate. Can transport glucose-6-phosphate, fructose-6-phosphate and mannose-6-phosphate. Also catalyzes the neutral exchange of internal and external phosphate. KEYWORDS: Cell inner membrane;Cell membrane;Membrane;Phosphate transport;Reference proteome;Sugar transport;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell inner membrane ; Multi-pass membrane protein " P0AGE6,"PROTEIN NAMES: Quinone reductase (Chromate reductase) (CHRR) (NAD(P)H dehydrogenase (quinone)) PROTEIN FAMILY: SsuE family ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the SsuE family. FUNCTION: Catalyzes the reduction of quinones. Acts by simultaneous two-electron transfer, avoiding formation of highly reactive semiquinone intermediates and producing quinols that promote tolerance of H(2)O(2). Quinone reduction is probably the primary biological role of ChrR (By similarity). Can also reduce toxic chromate to insoluble and less toxic Cr(3+). Catalyzes the transfer of three electrons to Cr(6+) producing Cr(3+) and one electron to molecular oxygen without producing the toxic Cr(5+) species and only producing a minimal amount of reactive oxygen species (ROS). Chromate reduction protects the cell against chromate toxicity, but is likely a secondary activity. Can also reduce potassium ferricyanide, 2,6-dichloroindophenol, V(5+), Mo(6+), methylene blue, cytochrome c and U(6+). During chromate reduction, is able to use both NAD or NADP equally well. KEYWORDS: 3D-structure;Flavoprotein;FMN;NAD;NADP;Oxidoreductase;Reference proteome " P0AGF2,PROTEIN NAMES: Sulfur acceptor protein CsdE PROTEIN FAMILY: SufE family ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the SufE family. FUNCTION: Stimulates the cysteine desulfurase activity of CsdA. Contains a cysteine residue (Cys-61) that acts to accept sulfur liberated via the desulfurase activity of CsdA. May be able to transfer sulfur to TcdA/CsdL. Seems to support the function of TcdA in the generation of cyclic threonylcarbamoyladenosine at position 37 (ct(6)A37) in tRNAs that read codons beginning with adenine. Does not appear to participate in Fe/S biogenesis. KEYWORDS: 3D-structure;Reference proteome P0AGG2,"PROTEIN NAMES: Acyl-CoA thioesterase 2 (Thioesterase II) (TEII) PROTEIN FAMILY: C/M/P thioester hydrolase family ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the C/M/P thioester hydrolase family. FUNCTION: Thioesterase that has relatively broad substrate specificity, hydrolyzing primarily medium- and long-chain acyl-CoA substrates to free fatty acids and CoA. Functions in the thioesterase-dependent pathway of beta-oxidation of oleate and conjugated linoleate ((9Z,11E)-octadecadienoate or CLA), which provides all energy and carbon precursors required for the growth of E.coli. Thus, supports growth on oleate or conjugated linoleate as the sole source of carbon by hydrolyzing 3,5-tetradecadienoyl-CoA, the terminal metabolite of oleate beta-oxidation via the alternative thioesterase-dependent pathway, and 3,5-dodecadienoyl-CoA, the end product of CLA beta-oxidation, respectively. Seems to be involved in 3-hydroxyalkanoate production in E.coli. KEYWORDS: 3D-structure;Direct protein sequencing;Hydrolase;Lipid metabolism;Reference proteome " P0AGM7,PROTEIN NAMES: Uracil permease (Uracil transporter) (Uracil/H(+) symporter UraA) PROTEIN FAMILY: Nucleobase:cation symporter-2 (NCS2) family ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) family. FUNCTION: Transport of uracil in the cell. KEYWORDS: 3D-structure;Cell inner membrane;Cell membrane;Membrane;Reference proteome;Symport;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell inner membrane ; Multi-pass membrane protein P0C024,"PROTEIN NAMES: Peroxisomal coenzyme A diphosphatase NUDT7 (Nucleoside diphosphate-linked moiety X motif 7) (Nudix motif 7) PROTEIN FAMILY: Nudix hydrolase family, PCD1 subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the Nudix hydrolase family. PCD1 subfamily. FUNCTION: Fatty acyl-coenzyme A (CoA) diphosphatase that hydrolyzes fatty acyl-CoA to yield acyl-4'-phosphopantetheine and adenosine 3',5'-bisphosphate (By similarity). Cleaves CoA, CoA esters and oxidized CoA with similar efficiencies (By similarity). Preferentially hydrolyzes medium-chain acyl-CoAs and bile acid-CoAs (By similarity). Has no activity toward NDP-sugars, CDP-alcohols, (deoxy)nucleoside 5'-triphosphates, nucleoside 5'-di or monophosphates, diadenosine polyphosphates, NAD, NADH, NADP, NADPH or thymidine-5'-monophospho-p-nitrophenyl ester (By similarity). May be required to eliminate oxidized CoA from peroxisomes, or regulate CoA and acyl-CoA levels in this organelle in response to metabolic demand (By similarity). Does not play a role in U8 snoRNA decapping activity (By similarity). Binds U8 snoRNA (By similarity). Exhibits decapping activity towards dpCoA-capped RNAs in vitro (By similarity). KEYWORDS: 3D-structure;Alternative splicing;Hydrolase;Magnesium;Manganese;Metal-binding;Peroxisome;Reference proteome;RNA-binding SUBCELLULAR LOCATION: Peroxisome " P0C0T0,"PROTEIN NAMES: Lipopolysaccharide-induced tumor necrosis factor-alpha factor homolog (LPS-induced TNF-alpha factor homolog) (Estrogen-enhanced transcript protein 1) (Eet-1) PROTEIN FAMILY: CDIP1/LITAF family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the CDIP1/LITAF family. FUNCTION: Plays a role in endosomal protein trafficking and in targeting proteins for lysosomal degradation. Plays a role in targeting endocytosed EGFR and ERGG3 for lysosomal degradation, and thereby helps down-regulate downstream signaling cascades. Helps recruit the ESCRT complex components TSG101, HGS and STAM to cytoplasmic membranes. Probably plays a role in regulating protein degradation via its interaction with NEDD4. May also contribute to the regulation of gene expression in the nucleus. Binds DNA (in vitro) and may play a synergistic role with STAT6 in the nucleus in regulating the expression of various cytokines. May regulate the expression of numerous cytokines, such as TNF, CCL2, CCL5, CXCL1, IL1A and IL10. KEYWORDS: Cell membrane;Cytoplasm;DNA-binding;Endosome;Golgi apparatus;Lysosome;Membrane;Metal-binding;Nucleus;Reference proteome;Transcription;Transcription regulation;Zinc SUBCELLULAR LOCATION: Cytoplasm Nucleus Lysosome membrane ; Peripheral membrane protein ; Cytoplasmic side Early endosome membrane Late endosome membrane Endosome membrane ; Peripheral membrane protein ; Cytoplasmic side Cell membrane ; Peripheral membrane protein ; Cytoplasmic side Golgi apparatus membrane Note=Associated with membranes of lysosomes, early and late endosomes. Can translocate from the cytoplasm into the nucleus (By similarity). Detected at Schmidt-Lanterman incisures and in nodal regions of myelinating Schwann cells (By similarity). " P0C0T2,"PROTEIN NAMES: Ankyrin repeat and SAM domain-containing protein 6 (Polycystic kidney disease protein 1) (SamCystin) (Sterile alpha motif domain-containing protein 6) (SAM domain-containing protein 6) ORGANISM: Rattus norvegicus (Rat) FUNCTION: Required for renal function. KEYWORDS: ANK repeat;Cell projection;Cilium;Cytoplasm;Disease variant;Hydroxylation;Phosphoprotein;Reference proteome;Repeat SUBCELLULAR LOCATION: Cell projection, cilium Cytoplasm Note=Localizes to the proximal region of the primary cilium in the presence of INVS. " P0C2H4,PROTEIN NAMES: Dermatan-sulfate epimerase (DS epimerase) (Chondroitin-glucuronate 5-epimerase) PROTEIN FAMILY: Dermatan-sulfate isomerase family ORGANISM: Bos taurus (Bovine) SIMILARITY: Belongs to the dermatan-sulfate isomerase family. FUNCTION: Converts D-glucuronic acid to L-iduronic acid (IdoUA) residues. Plays an important role in the biosynthesis of the glycosaminoglycan/mucopolysaccharide dermatan sulfate. PATHWAY: Glycan metabolism; chondroitin sulfate biosynthesis.; PATHWAY: Glycan metabolism; heparan sulfate biosynthesis. KEYWORDS: Cytoplasmic vesicle;Endoplasmic reticulum;Glycoprotein;Golgi apparatus;Isomerase;Manganese;Membrane;Metal-binding;Microsome;Reference proteome;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein Golgi apparatus membrane ; Multi-pass membrane protein Cytoplasmic vesicle membrane ; Multi-pass membrane protein Microsome membrane ; Multi-pass membrane protein P0C2N6,"PROTEIN NAMES: Histone-lysine N-methyltransferase KMT5C (Lysine-specific methyltransferase 5C) (Suppressor of variegation 4-20 homolog 2) (Su(var)4-20 homolog 2) (Suv4-20h2) ([histone H4]-N-methyl-L-lysine20 N-methyltransferase KMT5B) ([histone H4]-lysine20 N-methyltransferase KMT5B) PROTEIN FAMILY: Class V-like SAM-binding methyltransferase superfamily, Histone-lysine methyltransferase family, Suvar4-20 subfamily ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. Suvar4-20 subfamily. FUNCTION: Histone methyltransferase that specifically methylates monomethylated 'Lys-20' (H4K20me1) and dimethylated 'Lys-20' (H4K20me2) of histone H4 to produce respectively dimethylated 'Lys-20' (H4K20me2) and trimethylated 'Lys-20' (H4K20me3) and thus regulates transcription and maintenance of genome integrity. In vitro also methylates unmodified 'Lys-20' (H4K20me0) of histone H4 and nucleosomes (By similarity). H4 'Lys-20' trimethylation represents a specific tag for epigenetic transcriptional repression. Mainly functions in pericentric heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin in these regions. KMT5C is targeted to histone H3 via its interaction with RB1 family proteins (RB1, RBL1 and RBL2) (By similarity). Facilitates TP53BP1 foci formation upon DNA damage and proficient non-homologous end-joining (NHEJ)-directed DNA repair by catalyzing the di- and trimethylation of 'Lys-20' of histone H4 (By similarity). May play a role in class switch reconbination by catalyzing the di- and trimethylation of 'Lys-20' of histone H4 (By similarity). KEYWORDS: Chromatin regulator;Chromosome;Metal-binding;Methyltransferase;Nucleus;Reference proteome;Repressor;S-adenosyl-L-methionine;Transcription;Transcription regulation;Transferase;Zinc SUBCELLULAR LOCATION: Nucleus. Chromosome. Note=Associated with pericentric heterochromatin. CBX1 and CBX5 are required for the localization to pericentric heterochromatin. " P0C5H5,"PROTEIN NAMES: Gibberellin 20 oxidase 2 (GA 20-oxidase 2) (Gibberellin C-20 oxidase 2) (Os20ox2) (Protein semidwarf-1) PROTEIN FAMILY: Iron/ascorbate-dependent oxidoreductase family, GA20OX subfamily ORGANISM: Oryza sativa subsp. indica (Rice) SIMILARITY: Belongs to the iron/ascorbate-dependent oxidoreductase family. GA20OX subfamily. FUNCTION: Key oxidase enzyme in the biosynthesis of gibberellin that catalyzes the conversion of GA53 to GA20 via a three-step oxidation at C-20 of the GA skeleton. KEYWORDS: Iron;Metal-binding;Oxidoreductase MISCELLANEOUS: A second gene, 20ox1, is preferentially expressed in the reproductive organs and enables the flowers in sd1 plants to develop and be fertilized normally." P0C606,"PROTEIN NAMES: SAGA-associated factor 29 (rSGF29) (Coiled-coil domain-containing protein 101) (SAGA complex-associated factor 29) PROTEIN FAMILY: SGF29 family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the SGF29 family. FUNCTION: Chromatin reader component of some histone acetyltransferase (HAT) SAGA-type complexes like the TFTC-HAT, ATAC or STAGA complexes (By similarity). SGF29 specifically recognizes and binds methylated 'Lys-4' of histone H3 (H3K4me), with a preference for trimethylated form (H3K4me3) (By similarity). In the SAGA-type complexes, SGF29 is required to recruit complexes to H3K4me (By similarity). Involved in the response to endoplasmic reticulum (ER) stress by recruiting the SAGA complex to H3K4me, thereby promoting histone H3 acetylation and cell survival (By similarity). Also binds non-histone proteins that are methylated on Lys residues: specifically recognizes and binds CGAS monomethylated on 'Lys-491' (By similarity). May be involved in MYC-mediated oncogenic transformation. KEYWORDS: Acetylation;Chromatin regulator;Coiled coil;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " P0C7R1,"PROTEIN NAMES: Pentatricopeptide repeat-containing protein DWY1, chloroplastic (DYW-domain protein 1) PROTEIN FAMILY: PPR family, PCMP-H subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the PPR family. PCMP-H subfamily. FUNCTION: Plays a major role in single RNA editing events in chloroplasts. Acts as a site-recognition transacting factor involved in the edition of the site 1 of ndhD (ndhD-1 site corresponding to cytidine-2), which is a plastid-encoded subunit of the NADH-plastoquinone oxidoreductase. The interaction with CRR4 is required for its function in editing the ndhD-1 site. KEYWORDS: 3D-structure;Chloroplast;Metal-binding;mRNA processing;Plastid;Reference proteome;RNA editing;Transit peptide;Zinc SUBCELLULAR LOCATION: Plastid, chloroplast MISCELLANEOUS: Unlike other RNA editing factors, DYW1 does not contain identifiable PPR repeats but does contain E(+) and DYW motifs. Therefore its association with CCR4, which lacks E(+) and DYW motifs, but does contain PPR repeats, is required for its function in RNA editing." P0C8G6,"PROTEIN NAMES: Perivitellin-2 67 kDa subunit (PcPV2 67 kDa subunit) (PcPV2-67) (PV2 MACPF subunit) ORGANISM: Pomacea canaliculata (Golden apple snail) FUNCTION: The egg defensive protein perivitellin-2 is a pore-forming two-subunit glycoprotein that affects both the nervous and digestive systems of mammals. In addition, it is a source of both structural and energetic molecules during embryonic development (Probable). The tachylectin subunit (31 kDa) binds target membranes while the MACPF subunit (67 kDa) disrupts lipid bilayers forming large pores altering the plasma membrance conductance. Both in vivo and in vitro, the protein shows wide pH range stability and is resistant to enzymatic proteolysis from gastrointestinal environments. It specifically binds mature enterocytes but does not cause cell disruption on caco-2 (human colorectal adenocarcinoma cells) or rat intestinal cells. After oral administration to mice, it binds enterocytes and induces large dose-dependent morphological changes on their small intestine mucosa, reducing the absorptive surface (By similarity). Additionally, it is detected in the Peyer's patches where it activates lymphoid follicles and triggers apoptosis (By similarity). The toxin can also traverse the intestinal barrier and induce oral adaptive immunity with evidence of circulating antibody response. The toxin also shows hemagglutination properties thanks to the tachylectin subunit, but does not show hemolytic activity. In addition to enterotoxin activity, the toxin also acts as a neurotoxin, since an intraperitoneal injection induces paralysis of the mice rear limbs, followed by death. KEYWORDS: Direct protein sequencing;Disulfide bond;Enterotoxin;Glycoprotein;Hemagglutinin;Lipoprotein;Membrane;Neurotoxin;Secreted;Signal;Storage protein;Target cell membrane;Target membrane;Toxin SUBCELLULAR LOCATION: Secreted Target cell membrane " P0CB42,"PROTEIN NAMES: Nucleic acid dioxygenase ALKBH1 (Alkylated DNA repair protein alkB homolog 1) (Alpha-ketoglutarate-dependent dioxygenase ABH1) (DNA 6mA demethylase) (DNA N6-methyl adenine demethylase ALKBH1) (DNA lyase ABH1) (DNA oxidative demethylase ALKBH1) (mRNA N(3)-methylcytidine demethylase) (tRNA N1-methyl adenine demethylase) ORGANISM: Mus musculus (Mouse) FUNCTION: Dioxygenase that acts as on nucleic acids, such as DNA and tRNA. Requires molecular oxygen, alpha-ketoglutarate and iron. A number of activities have been described for this dioxygenase, but recent results suggest that it mainly acts as on tRNAs and mediates their demethylation or oxidation depending on the context and subcellular compartment (By similarity). Mainly acts as a tRNA demethylase by removing N(1)-methyladenine from various tRNAs, with a preference for N(1)-methyladenine at position 58 (m1A58) present on a stem loop structure of tRNAs. Acts as a regulator of translation initiation and elongation in response to glucose deprivation: regulates both translation initiation, by mediating demethylation of tRNA(Met), and translation elongation, N(1)-methyladenine-containing tRNAs being preferentially recruited to polysomes to promote translation elongation (By similarity). In mitochondrion, specifically interacts with mt-tRNA(Met) and mediates oxidation of mt-tRNA(Met) methylated at cytosine(34) to form 5-formylcytosine (f(5)c) at this position (By similarity). mt-tRNA(Met) containing the f(5)c modification at the wobble position enables recognition of the AUA codon in addition to the AUG codon, expanding codon recognition in mitochondrial translation (By similarity). Specifically demethylates DNA methylated on the 6th position of adenine (N(6)-methyladenosine) DNA. N(6)-methyladenosine (m6A) DNA is present at some L1 elements in embryonic stem cells and probably promotes their silencing. Demethylates mRNAs containing N(3)-methylcytidine modification (By similarity). Also able to repair alkylated single-stranded DNA by oxidative demethylation, but with low activity (By similarity). Also has DNA lyase activity and introduces double-stranded breaks at abasic sites: cleaves both single-stranded DNA and double-stranded DNA at abasic sites, with the greatest activity towards double-stranded DNA with two abasic sites (By similarity). DNA lyase activity does not require alpha-ketboglutarate and iron and leads to the formation of an irreversible covalent protein-DNA adduct with the 5' DNA product (By similarity). DNA lyase activity is not required during base excision repair and class switch recombination of the immunoglobulin heavy chain during B lymphocyte activation. May play a role in placental trophoblast lineage differentiation. KEYWORDS: 3D-structure;Dioxygenase;DNA damage;DNA repair;Iron;Lyase;Metal-binding;Multifunctional enzyme;Nucleus;Oxidoreductase;Reference proteome;RNA repair;Translation regulation SUBCELLULAR LOCATION: Nucleus Note=Mainly localizes in euchromatin, largely excluded from heterochromatin and nucleoli. " P0CP70,"PROTEIN NAMES: Serine/threonine-protein kinase ATG1 (Autophagy-related protein 1) PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family, APG1/unc-51/ULK1 subfamily ORGANISM: Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) (Filobasidiella neoformans) SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily. FUNCTION: Serine/threonine protein kinase involved in the cytoplasm to vacuole transport (Cvt) and found to be essential in autophagy, where it is required for the formation of autophagosomes. Involved in the clearance of protein aggregates which cannot be efficiently cleared by the proteasome. Required for selective autophagic degradation of the nucleus (nucleophagy) as well as for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Also involved in endoplasmic reticulum-specific autophagic process, in selective removal of ER-associated degradation (ERAD) substrates. Plays a key role in ATG9 and ATG23 cycling through the pre-autophagosomal structure and is necessary to promote ATG18 binding to ATG9 through phosphorylation of ATG9. Catalyzes phosphorylation of ATG4, decreasing the interaction between ATG4 and ATG8 and impairing deconjugation of PE-conjugated forms of ATG8. KEYWORDS: ATP-binding;Autophagy;Cytoplasm;Kinase;Membrane;Nucleotide-binding;Protein transport;Reference proteome;Serine/threonine-protein kinase;Transferase;Transport SUBCELLULAR LOCATION: Cytoplasm Preautophagosomal structure membrane ; Peripheral membrane protein " P0CS76,"PROTEIN NAMES: Serine/threonine-protein kinase SSN3 (Cyclin-dependent kinase 8) PROTEIN FAMILY: Protein kinase superfamily, CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily ORGANISM: Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) (Filobasidiella neoformans) SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. FUNCTION: Component of the SRB8-11 complex. The SRB8-11 complex is a regulatory module of the Mediator complex which is itself involved in regulation of basal and activated RNA polymerase II-dependent transcription. The SRB8-11 complex may be involved in the transcriptional repression of a subset of genes regulated by Mediator. It may inhibit the association of the Mediator complex with RNA polymerase II to form the holoenzyme complex. The SRB8-11 complex phosphorylates the C-terminal domain (CTD) of the largest subunit of RNA polymerase II (By similarity). KEYWORDS: Activator;ATP-binding;Kinase;Magnesium;Metal-binding;Nucleotide-binding;Nucleus;Reference proteome;Repressor;Serine/threonine-protein kinase;Transcription;Transcription regulation;Transferase SUBCELLULAR LOCATION: Nucleus " P0CY06,"PROTEIN NAMES: Mating-type protein ALPHA1 (MATalpha1 protein) (Alpha-1 activator) PROTEIN FAMILY: MATALPHA1 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the MATALPHA1 family. FUNCTION: Mating type proteins are sequence specific DNA-binding proteins that act as master switches in yeast differentiation by controlling gene expression in a cell type-specific fashion. Transcriptional coactivator that, in alpha-cells, binds cooperatively with MCM1 and STE12 to a DNA sequence termed the QP' element, to activate the transcription of alpha-specific genes. KEYWORDS: Activator;DNA-binding;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: There are three genetic loci for mating type genes in S.cerevisiae. MAT is the expression locus that determines the mating type of the cell, whereas HML (containing HMLALPHA1 and HMLALPHA2) and HMR (containing HMRA1 and HMRA2) represent silenced repositories of mating type information. The mating type is determined by the MAT locus, which contains either a copy of HML or of HMR. Diploid cells are usually heterozygous for the MAT locus." P0CY08,"PROTEIN NAMES: Mating-type protein ALPHA2 (MATalpha2 protein) (Alpha-2 repressor) PROTEIN FAMILY: TALE/M-ATYP homeobox family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the TALE/M-ATYP homeobox family. FUNCTION: Mating type proteins are sequence specific DNA-binding proteins that act as master switches in yeast differentiation by controlling gene expression in a cell type-specific fashion. Transcriptional corepressor that binds cooperatively with MCM1 to a 31-basepair DNA sequence termed the a-specific gene (asg) operator, to repress the transcription of a-cell-specific genes. Additionally, in a/alpha diploid cells, binds cooperatively with the A1 protein to a 21-basepair DNA sequence termed the haploid-specific gene (hsg) operator, to repress transcription of haploid-specific genes and of MATALPHA1. KEYWORDS: 3D-structure;Acetylation;Direct protein sequencing;DNA-binding;Homeobox;Nucleus;Reference proteome;Repressor;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus. MISCELLANEOUS: There are three genetic loci for mating type genes in S.cerevisiae. MAT is the expression locus that determines the mating type of the cell, whereas HML (containing HMLALPHA1 and HMLALPHA2) and HMR (containing HMRA1 and HMRA2) represent silenced repositories of mating type information. The mating type is determined by the MAT locus, which contains either a copy of HML or of HMR. Diploid cells are usually heterozygous for the MAT locus." P0CY34,"PROTEIN NAMES: Transcriptional repressor TUP1 ORGANISM: Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) FUNCTION: Represses transcription by RNA polymerase II. Represses genes responsible for initiating filamentous growth such as HWP1, RBT1, RBT2, RBT4, RBT5, RBT7 and WAP1; and this repression is lifted under inducing environmental conditions. Represses also genes which participate in pathogenesis. Crucial component of the response to farnesol. Plays an important role in the regulation of white-opaque switching. KEYWORDS: Reference proteome;Repeat;Repressor;Transcription;Transcription regulation;Virulence;WD repeat " P0DJE9,"PROTEIN NAMES: Thrombin-like enzyme TLBm (SVTLE TLBm) (Fibrinogen-clotting enzyme) (Snake venom serine protease) (SVSP) PROTEIN FAMILY: Peptidase S1 family, Snake venom subfamily ORGANISM: Bothrops marajoensis (Marajo lancehead) SIMILARITY: Belongs to the peptidase S1 family. Snake venom subfamily. FUNCTION: Thrombin-like enzyme that induces the formation of fibrin clot. Cleaves the Aalpha-chain of fibrinogen (FGA) with higher activity than the Bbeta-chain (FGB). Induces platelet aggregation in both platelet-rich plasma and in washed platelet preparations. This aggregation is strongly inhibited by preincubation of the enzyme with PMSF. KEYWORDS: Blood coagulation cascade activating toxin;Direct protein sequencing;Disulfide bond;Hemostasis impairing toxin;Hydrolase;Platelet aggregation activating toxin;Protease;Secreted;Serine protease;Toxin SUBCELLULAR LOCATION: Secreted. MISCELLANEOUS: Negative results: has no activity on the gamma-chain of fibrinogen (FGG). In vivo, does not induce a significant edema activity in mice." P0DKI7,"PROTEIN NAMES: Bifunctional protein STORR ((S)- to (R)-reticuline) [Includes: Cytochrome P450 82Y2 (1,2-dehydroreticuline synthase) (CYP82Y2); Oxydoreductase (1,2-dehydroreticulinium reductase (NADPH))] PROTEIN FAMILY: Cytochrome P450 family; Aldo/keto reductase family ORGANISM: Papaver somniferum (Opium poppy) SIMILARITY: In the N-terminal section; belongs to the cytochrome P450 family.; SIMILARITY: In the C-terminal section; belongs to the aldo/keto reductase family. FUNCTION: Bifunctional protein involved in the biosynthesis of morphinan-type benzylisoquinoline alkaloids. Required for the isomerization of (S)- to (R)-reticuline. The cytochrome P450 module is responsible for the conversion of (S)-reticuline to 1,2-dehydroreticuline while the oxidoreductase module converts 1,2-dehydroreticuline to (R)-reticuline. PATHWAY: Alkaloid biosynthesis; morphine biosynthesis. KEYWORDS: Alkaloid metabolism;Heme;Iron;Membrane;Metal-binding;Multifunctional enzyme;NADP;Oxidoreductase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Single-pass membrane protein " P0DMC7,"PROTEIN NAMES: Transcriptional regulatory protein RcsB (Capsular synthesis regulator component B) PROTEIN FAMILY: RcsB family ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the RcsB family. FUNCTION: Component of the Rcs signaling system, which controls transcription of numerous genes. RcsB is the response regulator that binds to regulatory DNA regions. Can function both in an RcsA-dependent or RcsA-independent manner. The system regulates expression of numerous genes, including genes involved in colanic acid capsule synthesis, biofilm formation, cell division and outer membrane proteins synthesis. Also involved, with GadE, in control of glutamate-dependent acid resistance, and, with BglJ, in derepression of the cryptic bgl operon. The RcsB-BglJ activity is probably independent of RcsB phosphorylation. KEYWORDS: 3D-structure;Activator;Capsule biogenesis/degradation;DNA-binding;Phosphoprotein;Reference proteome;Transcription;Transcription regulation;Two-component regulatory system MISCELLANEOUS: There is a close linkage between the Rcs and PhoQ/P signaling systems, and both signaling systems respond to certain external divalent cations (zinc and magnesium)." P0DPA8,"PROTEIN NAMES: 4-hydroxytryptamine kinase (Psilocybin biosynthesis kinase) PROTEIN FAMILY: Methylthioribose kinase family ORGANISM: Psilocybe cubensis (Psychedelic mushroom) (Stropharia cubensis) SIMILARITY: Belongs to the methylthioribose kinase family. FUNCTION: 4-hydroxytryptamine kinase; part of the gene cluster that mediates the biosynthesis of psilocybin, a psychotropic tryptamine-derived natural product. The first step in the pathway is the decarboxylation of L-tryptophan to tryptamine by the decarboxylase psiD. 4-hydroxy-L-tryptophan is accepted as substrate by psiD as well. The cytochrome P450 monooxygenase psiH then converts tryptamine to 4-hydroxytryptamine. The kinase psiK catalyzes the 4-O-phosphorylation step by converting 4-hydroxytryptamine into norbaeocystin. The methyltransferase psiM then catalyzes iterative methyl transfer to the amino group of norbaeocystin to yield psilocybin via a monomethylated intermediate, baeocystin. 4-hydroxy-6-methyl-l-tryptophancan also be converted the decarboxylase PsiD, kinase PsiK, and methyltransferase PsiM into respectively 6-methyl-norbaeocystin, 6-methylbaeocystin, and 6-methylpsilocybin. PsiK kinase can also turn psilocin into psilocybin. This activity may represent a protective mechanism to rephosphorylate the unstable psilocin to the stable psilocybin in case of intracellular ester cleavage. Moreover, psiK is able to O-phosphorylate the quaternary amine 4-hydroxy-N,N,N-trimethyltryptamine (4-OH-TMT) to yield aeruginascin, another bioactive compound found in Psilocybe species. PATHWAY: Secondary metabolite biosynthesis. KEYWORDS: ATP-binding;Kinase;Magnesium;Nucleotide-binding;Transferase " P0DPR2,"PROTEIN NAMES: E3 ubiquitin-protein ligase RNF43 (RING finger protein 43) (RING-type E3 ubiquitin transferase RNF43) PROTEIN FAMILY: ZNRF3 family ORGANISM: Xenopus tropicalis (Western clawed frog) (Silurana tropicalis) SIMILARITY: Belongs to the ZNRF3 family. FUNCTION: E3 ubiquitin-protein ligase that acts as a negative regulator of the Wnt signaling pathway by mediating the ubiquitination, endocytosis and subsequent degradation of Wnt receptor complex components Frizzled. Acts on both canonical and non-canonical Wnt signaling pathway (By similarity). Along with RSPO2 and ZNRF3, constitutes a master switch that governs limb specification. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Cell membrane;Developmental protein;Disulfide bond;Endoplasmic reticulum;Glycoprotein;Membrane;Metal-binding;Nucleus;Reference proteome;Signal;Transferase;Transmembrane;Transmembrane helix;Ubl conjugation pathway;Wnt signaling pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein Endoplasmic reticulum membrane ; Single-pass type I membrane protein Nucleus envelope " P0DSP3,"PROTEIN NAMES: Cyclic dipyrimidine nucleotide synthase CdnE (LpCdnE) (Cyclic CMP-UMP synthase) (c-di-UMP synthase) (cGAS/DncV-like nucleotidyltransferase) (CD-NTase057) (Lp-CdnE02) PROTEIN FAMILY: CD-NTase family, E02 subfamily ORGANISM: Legionella pneumophila SIMILARITY: Belongs to the CD-NTase family. E02 subfamily. FUNCTION: Cyclic nucleotide synthase (second messenger synthase) of a CBASS antivirus system. CBASS (cyclic oligonucleotide-based antiphage signaling system) provides immunity against bacteriophage. The CD-NTase protein synthesizes cyclic nucleotides in response to infection; these serve as specific second messenger signals. The signals activate a diverse range of effectors, leading to bacterial cell death and thus abortive phage infection (Probable). A type I-B(UU) CBASS system.; FUNCTION: Cyclic dinucleotide synthase that catalyzes the synthesis of 3',3'-cyclic UMP-UMP (c-di-UMP) as the major product, and of 3',3'-cyclic CMP-UMP as a minor product, which are second messengers for cell signal transduction. KEYWORDS: 3D-structure;Antiviral defense;Magnesium;Metal-binding;Nucleotide metabolism;Nucleotide-binding;Nucleotidyltransferase;Transferase " P0DSP4,"PROTEIN NAMES: Cyclic AMP-AMP-GMP synthase (cGAS/DncV-like nucleotidyltransferase) (CD-NTase038) (EcCdnD) PROTEIN FAMILY: CD-NTase family, D02 subfamily ORGANISM: Enterobacter hormaechei subsp. hoffmannii (strain UCI 50) SIMILARITY: Belongs to the CD-NTase family. D02 subfamily. FUNCTION: Cyclic nucleotide synthase (second messenger synthase) of a CBASS antivirus system. CBASS (cyclic oligonucleotide-based antiphage signaling system) provides immunity against bacteriophages. The CD-NTase protein (CdnD, this protein) synthesizes cyclic nucleotides in response to infection; these serve as specific second messenger signals. The signals activate a diverse range of effectors, leading to bacterial cell death and thus abortive phage infection. A type II-C(AAG) CBASS system.; FUNCTION: Cyclic trinucleotide synthase that catalyzes the synthesis of 3',3',3'-cyclic AMP-AMP-GMP (cAAG) as the major product, a second messenger for cell signal transduction. Uses ATP as the first donor nucleotide, followed by GTP.; FUNCTION: Protects E.coli against phage T2 infection. When the cdnD-cap2-cap3-cap4 operon is introduced in E.coli there is a more than 10(3) decrease in the efficiency of T2 plaque formation. The operon does not protect against phage T5 and only about 10-fold against T7. Expression of cdnD-cap4 alone protects E.coli against phage T2 infection. KEYWORDS: 3D-structure;Antiviral defense;ATP-binding;GTP-binding;Isopeptide bond;Magnesium;Metal-binding;Nucleotide metabolism;Nucleotide-binding;Nucleotidyltransferase;Transferase " P0DTE5,"PROTEIN NAMES: UDP-glucuronosyltransferase 2A2 (UDPGT 2A2) PROTEIN FAMILY: UDP-glycosyltransferase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the UDP-glycosyltransferase family. FUNCTION: UDP-glucuronosyltransferase (UGT) that catalyzes phase II biotransformation reactions in which lipophilic substrates are conjugated with glucuronic acid to increase the metabolite's water solubility, thereby facilitating excretion into either the urine or bile. Essential for the elimination and detoxification of drugs, xenobiotics and endogenous compounds. Catalyzes the glucuronidation of endogenous estrogen hormone estradiol. Contributes to bile acid (BA) detoxification by catalyzing the glucuronidation of BA substrates, which are natural detergents for dietary lipids absorption. Shows a potential role in detoxification of toxic waste compounds in the amniotic fluid before birth, and air-born chemical after birth. KEYWORDS: Alternative splicing;Endoplasmic reticulum;Glycoprotein;Glycosyltransferase;Lipid metabolism;Membrane;Reference proteome;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein MISCELLANEOUS: UGT2A2 isoform is part of the UGT2A complex locus which displays alternative use of promoters and exons. The locus is defined by 2 alternative promoters resulting in 2 functionally active polypeptides UGT2A1 and UGT2A2. Alternative splicing of exons results in additional isoforms for each protein class." P0DTQ0,"PROTEIN NAMES: 5-deoxy-D-ribulose 1-phosphate aldolase (5-deoxyribose disposal aldolase) PROTEIN FAMILY: Aldolase class II family ORGANISM: Bacillus thuringiensis serovar kurstaki (strain ATCC 35866 / NRRL B-4488 / HD73) SIMILARITY: Belongs to the aldolase class II family. FUNCTION: Catalyzes the cleavage of 5-deoxy-D-ribulose 1-phosphate to yield dihydroxyacetone phosphate (DHAP) and acetaldehyde, as part of a 5-deoxyribose salvage pathway that recycles this toxic radical SAM enzyme by-product to mainstream metabolites. Is also able to catalyze the reverse reaction, using several aldehydes as substrate, with acetaldehyde being the preferred substrate. PATHWAY: Carbohydrate degradation. KEYWORDS: 3D-structure;Lyase;Manganese;Metal-binding " P0DUJ8,"PROTEIN NAMES: Protein-arginine N-acetylglucosaminyltransferase SseK2 (Arginine GlcNAcyltransferase SseK2) (Salmonella secreted effector K2) PROTEIN FAMILY: Glycosyltransferase NleB family ORGANISM: Salmonella typhimurium (strain SL1344) SIMILARITY: Belongs to the glycosyltransferase NleB family. FUNCTION: Protein-arginine N-acetylglucosaminyltransferase effector that catalyzes the transfer of a single N-acetylglucosamine (GlcNAc) to a conserved arginine residue in the death domain of host proteins such as FADD: arginine GlcNAcylation prevents homotypic/heterotypic death domain interactions. Also acts on host proteins without a death domain: catalyzes arginine GlcNAcylation of host small Rab1 GTPase, thereby preventing GTPase activity and leading to impaired host vesicular protein transport. In contrast to Ssek1, not able to disrupt TNF signaling in infected cells (By similarity). KEYWORDS: 3D-structure;Glycosyltransferase;Host Golgi apparatus;Manganese;Metal-binding;Secreted;Toxin;Transferase;Virulence SUBCELLULAR LOCATION: Secreted Host Golgi apparatus Note=Secreted via the type III secretion system (T3SS). " P10105,"PROTEIN NAMES: Homeotic protein labial (F24) (F90-2) PROTEIN FAMILY: Antp homeobox family, Labial subfamily ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the Antp homeobox family. Labial subfamily. FUNCTION: Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Required for proper head development. KEYWORDS: Alternative splicing;Developmental protein;DNA-binding;Homeobox;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus. " P10152,"PROTEIN NAMES: Angiogenin-1 PROTEIN FAMILY: Pancreatic ribonuclease family ORGANISM: Bos taurus (Bovine) SIMILARITY: Belongs to the pancreatic ribonuclease family. FUNCTION: Binds to actin on the surface of endothelial cells; once bound, angiogenin is endocytosed and translocated to the nucleus. Stimulates ribosomal RNA synthesis including that containing the initiation site sequences of 45S rRNA. Cleaves tRNA within anticodon loops to produce tRNA-derived stress-induced fragments (tiRNAs) which inhibit protein synthesis and triggers the assembly of stress granules (SGs) (By similarity). Angiogenin induces vascularization of normal and malignant tissues. Angiogenic activity is regulated by interaction with RNH1 in vivo. Has very low ribonuclease activity. KEYWORDS: 3D-structure;Angiogenesis;Cytoplasmic vesicle;Developmental protein;Differentiation;Direct protein sequencing;Disulfide bond;DNA-binding;Endonuclease;Hydrolase;Nuclease;Nucleus;Protein synthesis inhibitor;Reference proteome;Secreted;Signal;Stress response SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle lumen Secreted Nucleus, nucleolus Note=Rapidly endocytosed by target cells and translocated to the nucleus where it accumulates in the nucleolus and binds to DNA (By similarity). " P10153,PROTEIN NAMES: Non-secretory ribonuclease (Eosinophil-derived neurotoxin) (RNase UpI-2) (Ribonuclease 2) (RNase 2) (Ribonuclease US) PROTEIN FAMILY: Pancreatic ribonuclease family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the pancreatic ribonuclease family. FUNCTION: This is a non-secretory ribonuclease. It is a pyrimidine specific nuclease with a slight preference for U. Cytotoxin and helminthotoxin. Selectively chemotactic for dendritic cells. Possesses a wide variety of biological activities. KEYWORDS: 3D-structure;Chemotaxis;Direct protein sequencing;Disulfide bond;Endonuclease;Glycoprotein;Hydrolase;Lyase;Lysosome;Nitration;Nuclease;Reference proteome;Signal SUBCELLULAR LOCATION: Lysosome Cytoplasmic granule. Note=Matrix of eosinophil's large specific granule. P10184,"PROTEIN NAMES: Ovoinhibitor (OI) (OvoI) (Serine protease inhibitor Kazal-type 5) (allergen Gal d OIH) ORGANISM: Gallus gallus (Chicken) FUNCTION: Serine protease inhibitor involved in antimicrobial egg defense preventing contamination of table eggs (non-fertilized eggs) and protecting the chick embryo (fertilized eggs) (Probable). Inhibits trypsin, chymotrypsin, elastase, subtilisin and a proteinase of fungus Aspergillus oryzae. Inhibits calcium-activated potassium channels KCNMA1 (bovine) and slo (Drosophila). Has antibacterial activity against B.thuringiensis LMSA 3.06.004, but not against S.aureus CIP 103 811, P.aeruginosa PAO1, B.cereus ATCC6464 or B.subtilis ATCC 6633. KEYWORDS: Allergen;Alternative splicing;Antimicrobial;Direct protein sequencing;Disulfide bond;Glycoprotein;Protease inhibitor;Reference proteome;Repeat;Secreted;Serine protease inhibitor SUBCELLULAR LOCATION: Secreted MISCELLANEOUS: Prevents symptomatic gastoroenteritis in vivo in a mouse model of rotavirus infection. Significantly inhibits intestinal replication of EDIM strain of murine rotavirus in infant mice up to 4 days after intragastrical administration of the virus." P10275,"PROTEIN NAMES: Androgen receptor (Dihydrotestosterone receptor) (Nuclear receptor subfamily 3 group C member 4) PROTEIN FAMILY: Nuclear hormone receptor family, NR3 subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the nuclear hormone receptor family. NR3 subfamily. FUNCTION: Steroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Transcription factor activity is modulated by bound coactivator and corepressor proteins like ZBTB7A that recruits NCOR1 and NCOR2 to the androgen response elements/ARE on target genes, negatively regulating androgen receptor signaling and androgen-induced cell proliferation. Transcription activation is also down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3 and ZIPK/DAPK3.; FUNCTION: [Isoform 3]: Lacks the C-terminal ligand-binding domain and may therefore constitutively activate the transcription of a specific set of genes independently of steroid hormones.; FUNCTION: [Isoform 4]: Lacks the C-terminal ligand-binding domain and may therefore constitutively activate the transcription of a specific set of genes independently of steroid hormones. KEYWORDS: 3D-structure;Activator;Alternative splicing;Cytoplasm;Disease variant;DNA-binding;Isopeptide bond;Lipid-binding;Lipoprotein;Metal-binding;Neurodegeneration;Nucleus;Palmitate;Phosphoprotein;Pseudohermaphroditism;Receptor;Reference proteome;Steroid-binding;Transcription;Transcription regulation;Triplet repeat expansion;Ubl conjugation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Cytoplasm Note=Detected at the promoter of target genes. Predominantly cytoplasmic in unligated form but translocates to the nucleus upon ligand-binding. Can also translocate to the nucleus in unligated form in the presence of RACK1. MISCELLANEOUS: In the absence of ligand, steroid hormone receptors are thought to be weakly associated with nuclear components; hormone binding greatly increases receptor affinity. The hormone-receptor complex appears to recognize discrete DNA sequences upstream of transcriptional start sites.; MISCELLANEOUS: Transcriptional activity is enhanced by binding to RANBP9.; MISCELLANEOUS: The level of tyrosine phosphorylation may serve as a diagnostic tool to predict patient outcome in response to hormone-ablation therapy. Inhibition of tyrosine phosphorylation may be an effective intervention target for hormone-refractory prostate cancer.; MISCELLANEOUS: [Isoform 3]: Minor isoform up-regulated in prostate cancer cells.; MISCELLANEOUS: [Isoform 4]: Minor isoform identified in prostate cancer cells." P10297,"PROTEIN NAMES: Antiviral protein I (PAP-C) (PAP-I) (PAP-R) (Ribosome-inactivating protein) (rRNA N-glycosidase) PROTEIN FAMILY: Ribosome-inactivating protein family, Type 1 RIP subfamily ORGANISM: Phytolacca americana (American pokeweed) (Phytolacca decandra) SIMILARITY: Belongs to the ribosome-inactivating protein family. Type 1 RIP subfamily. FUNCTION: Possesses antiviral potency. Inhibits viral infection of plants (tobacco mosaic virus). Inhibits protein synthesis. Releases both adenine and guanine from Escherichia coli rRNA in vitro. Activity on guanine is 20 times slower than that on adenine. KEYWORDS: 3D-structure;Antiviral defense;Direct protein sequencing;Disulfide bond;Hydrolase;Plant defense;Protein synthesis inhibitor;Signal;Toxin MISCELLANEOUS: Depurinates genomic RNA of immunodeficiency virus type-I (HIV-I) and bacteriophage (MS 2) RNA. Inhibits the replication of HIV-1 in human peripheral blood mononuclear cells with IC(50) value of 14 nM." P10300,"PROTEIN NAMES: T-cell surface glycoprotein CD8 beta chain (Lymphocyte antigen 3) (T-cell membrane glycoprotein Ly-3) (T-cell surface glycoprotein Lyt-3) (CD antigen CD8b) ORGANISM: Mus musculus (Mouse) FUNCTION: Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. A palmitoylation site in the cytoplasmic tail of CD8B chain contributes to partitioning of CD8 into the plasma membrane lipid rafts where signaling proteins are enriched. Once LCK recruited, it initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs). Additionally, plays a critical role in thymic selection of CD8+ T-cells. KEYWORDS: 3D-structure;Adaptive immunity;Disulfide bond;Glycoprotein;Immunity;Immunoglobulin domain;Membrane;Reference proteome;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Single-pass type I membrane protein. Note=Requires the partner CD8A for efficient cell surface expression. The heterodimer CD8A/CD8B localizes to lipid rafts due to CD8B cytoplasmic tail palmitoylation. " P10383,PROTEIN NAMES: Protein ovarian tumor locus ORGANISM: Drosophila melanogaster (Fruit fly) FUNCTION: Essential for female fertility; germ cell division and differentiation. KEYWORDS: Alternative splicing;Cytoplasm;Reference proteome SUBCELLULAR LOCATION: Cytoplasm P10415,"PROTEIN NAMES: Apoptosis regulator Bcl-2 PROTEIN FAMILY: Bcl-2 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the Bcl-2 family. FUNCTION: Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). Also acts as an inhibitor of autophagy: interacts with BECN1 and AMBRA1 during non-starvation conditions and inhibits their autophagy function. May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release. KEYWORDS: 3D-structure;Alternative splicing;Apoptosis;Autophagy;Chromosomal rearrangement;Cytoplasm;Disease variant;Endoplasmic reticulum;Membrane;Mitochondrion;Mitochondrion outer membrane;Nucleus;Phosphoprotein;Proto-oncogene;Reference proteome;Transmembrane;Transmembrane helix;Ubl conjugation SUBCELLULAR LOCATION: Mitochondrion outer membrane ; Single-pass membrane protein Nucleus membrane ; Single-pass membrane protein Endoplasmic reticulum membrane ; Single-pass membrane protein Cytoplasm " P10505,"PROTEIN NAMES: Anaphase-promoting complex subunit 3 (20S cyclosome/APC complex protein apc3) (Nuclear alteration protein 2) (Nuclear scaffold-like protein p76) PROTEIN FAMILY: APC3/CDC27 family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the APC3/CDC27 family. FUNCTION: Component of the anaphase-promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin-protein ligase complex that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C is thought to confer substrate specificity and, in the presence of ubiquitin-conjugating E2 enzymes, it catalyzes the formation of protein-ubiquitin conjugates that are subsequently degraded by the 26S proteasome. Interacts with spindle apparatus, chromosomes, or nuclear envelope, and interconnect nuclear and cytoskeletal functions in mitosis, so the elongation of the spindle in anaphase is blocked. KEYWORDS: Cell cycle;Cell division;Mitosis;Nucleus;Reference proteome;Repeat;TPR repeat;Ubl conjugation pathway SUBCELLULAR LOCATION: Nucleus. " P10547,PROTEIN NAMES: Lysostaphin (Glycyl-glycine endopeptidase) PROTEIN FAMILY: Peptidase M23B family ORGANISM: Staphylococcus simulans SIMILARITY: Belongs to the peptidase M23B family. FUNCTION: Lyses staphylococcal cells by hydrolyzing the polyglycine interpeptide bridges of the peptidoglycan. KEYWORDS: 3D-structure;Cell wall biogenesis/degradation;Hydrolase;Metal-binding;Metalloprotease;Protease;Repeat;Secreted;Signal;Zinc;Zymogen SUBCELLULAR LOCATION: Secreted. P10619,"PROTEIN NAMES: Lysosomal protective protein (Carboxypeptidase C) (Carboxypeptidase L) (Cathepsin A) (Protective protein cathepsin A) (PPCA) (Protective protein for beta-galactosidase) [Cleaved into: Lysosomal protective protein 32 kDa chain; Lysosomal protective protein 20 kDa chain] PROTEIN FAMILY: Peptidase S10 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the peptidase S10 family. FUNCTION: Protective protein appears to be essential for both the activity of beta-galactosidase and neuraminidase, it associates with these enzymes and exerts a protective function necessary for their stability and activity. This protein is also a carboxypeptidase and can deamidate tachykinins. KEYWORDS: 3D-structure;Alternative splicing;Carboxypeptidase;Direct protein sequencing;Disease variant;Disulfide bond;Glycoprotein;Hydrolase;Lysosome;Protease;Reference proteome;Signal;Zymogen SUBCELLULAR LOCATION: Lysosome. " P10674,"PROTEIN NAMES: Fasciclin-1 (Fasciclin I) (FAS I) (FCN) ORGANISM: Drosophila melanogaster (Fruit fly) FUNCTION: Neural cell adhesion molecule. KEYWORDS: 3D-structure;Alternative splicing;Cell adhesion;Cell membrane;Glycoprotein;GPI-anchor;Lipoprotein;Membrane;Reference proteome;Repeat;Signal SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor, GPI-anchor. " P10815,"PROTEIN NAMES: G2/mitotic-specific cyclin cdc13 PROTEIN FAMILY: Cyclin family, Cyclin AB subfamily ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the cyclin family. Cyclin AB subfamily. FUNCTION: Essential for the control of the cell cycle at the G2/M (mitosis) transition. Interacts with the cdc2 protein kinase to form MPF. G2/M cyclins accumulate steadily during G2 and are abruptly destroyed at mitosis. Involved in the reorganization of the cytoskeleton on transition from G2 to mitosis. Association with rum1 promotes its proteolysis during G1. Also essential for initiation of meiosis II. KEYWORDS: Cell cycle;Cell division;Cyclin;Cytoplasm;Cytoskeleton;Mitosis;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body " P11049,"PROTEIN NAMES: Leukocyte antigen CD37 (Tetraspanin-26) (Tspan-26) (CD antigen CD37) PROTEIN FAMILY: Tetraspanin (TM4SF) family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the tetraspanin (TM4SF) family. FUNCTION: Structural component of specialized membrane microdomains known as tetraspanin-enriched microdomains (TERMs), which act as platforms for receptor clustering and signaling. Participates thereby in diverse biological functions such as cell signal transduction, adhesion, migration and protein trafficking. Upon ligand binding, two signaling pathways are activated, one acting through phosphorylation by LYN leading to cell death or a survival pathway with activation of GSK3B. Plays an essential role essential for clustering of integrin ITGA4/ITGB1 and promotes its mobility in the plasma membrane of B-cells. In turn, participates in ITGA4/ITGB1 integrin-mediated antiapoptotic signaling through AKT (By similarity). Plays also a role in the migration of dendritic cells and neutrophils to draining lymph nodes, as well as in their integrin-mediated adhesion (By similarity). Negatively regulates IL-6 responses through direct interaction with SOCS3 thereby preventing constitutive IL-6 signaling. Alternatively, inhibition of IL-6 signaling can also occur via interaction and stabilization of DECTIN1/CLEC7A at the cell membrane to inhibit its ability to promote the production of IL-6. KEYWORDS: Alternative splicing;Cell membrane;Glycoprotein;Membrane;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein. " P11161,"PROTEIN NAMES: E3 SUMO-protein ligase EGR2 (AT591) (E3 SUMO-protein transferase ERG2) (Early growth response protein 2) (EGR-2) (Zinc finger protein Krox-20) PROTEIN FAMILY: EGR C2H2-type zinc-finger protein family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the EGR C2H2-type zinc-finger protein family. FUNCTION: Sequence-specific DNA-binding transcription factor. Plays a role in hindbrain segmentation by regulating the expression of a subset of homeobox containing genes and in Schwann cell myelination by regulating the expression of genes involved in the formation and maintenance of myelin (By similarity). Binds to two EGR2-consensus sites EGR2A (5'-CTGTAGGAG-3') and EGR2B (5'-ATGTAGGTG-3') in the HOXB3 enhancer and promotes HOXB3 transcriptional activation (By similarity). Binds to specific DNA sites located in the promoter region of HOXA4, HOXB2 and ERBB2 (By similarity). Regulates hindbrain segmentation by controlling the expression of Hox genes, such as HOXA4, HOXB3 and HOXB2, and thereby specifying odd and even rhombomeres (By similarity). Promotes the expression of HOXB3 in the rhombomere r5 in the hindbrain (By similarity). Regulates myelination in the peripheral nervous system after birth, possibly by regulating the expression of myelin proteins, such as MPZ, and by promoting the differentiation of Schwann cells (By similarity). Involved in the development of the jaw openener musculature, probably by playing a role in its innervation through trigeminal motor neurons (By similarity). May play a role in adipogenesis, possibly by regulating the expression of CEBPB (By similarity).; FUNCTION: E3 SUMO-protein ligase helping SUMO1 conjugation to its coregulators NAB1 and NAB2, whose sumoylation down-regulates EGR2 transcriptional activity. PATHWAY: Protein modification; protein sumoylation. KEYWORDS: Acetylation;Activator;Alternative splicing;Charcot-Marie-Tooth disease;Dejerine-Sottas syndrome;Disease variant;DNA-binding;Metal-binding;Neurodegeneration;Neuropathy;Nucleus;Reference proteome;Repeat;Transcription;Transcription regulation;Transferase;Ubl conjugation;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus " P11181,"PROTEIN NAMES: Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial (Branched-chain alpha-keto acid dehydrogenase complex component E2) (BCKAD-E2) (BCKADE2) (Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex) (Dihydrolipoamide branched chain transacylase) (Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase) PROTEIN FAMILY: 2-oxoacid dehydrogenase family ORGANISM: Bos taurus (Bovine) SIMILARITY: Belongs to the 2-oxoacid dehydrogenase family. FUNCTION: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3). Within this complex, the catalytic function of this enzyme is to accept, and to transfer to coenzyme A, acyl groups that are generated by the branched-chain alpha-keto acid decarboxylase component. KEYWORDS: 3D-structure;Acetylation;Acyltransferase;Direct protein sequencing;Lipoyl;Mitochondrion;Phosphoprotein;Reference proteome;Transferase;Transit peptide SUBCELLULAR LOCATION: Mitochondrion matrix " P11218,PROTEIN NAMES: Lectin/endochitinase 1 (Agglutinin) (UDA) (chia5.1.1) [Cleaved into: Lectin 1] ORGANISM: Urtica dioica (Great nettle) (Stinging nettle) FUNCTION: Functions both as a chitinase and as a N-acetyl-D-glucosamine binding lectin. Inhibits the growth of several phytopathogenic chitin-containing fungi. Possesses also insecticidal activity and superantigenic properties. KEYWORDS: 3D-structure;Antimicrobial;Carbohydrate metabolism;Chitin degradation;Chitin-binding;Direct protein sequencing;Disulfide bond;Fungicide;Glycoprotein;Glycosidase;Hydrolase;Lectin;Metal-binding;Plant defense;Polysaccharide degradation;Pyrrolidone carboxylic acid;Repeat;Signal;Zinc P11245,"PROTEIN NAMES: Arylamine N-acetyltransferase 2 (Arylamide acetylase 2) (N-acetyltransferase type 2) (NAT-2) (N-hydroxyarylamine O-acetyltransferase) (Polymorphic arylamine N-acetyltransferase) (PNAT) PROTEIN FAMILY: Arylamine N-acetyltransferase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the arylamine N-acetyltransferase family. FUNCTION: Catalyzes the N- or O-acetylation of various arylamine and heterocyclic amine substrates. Participates in the detoxification of a plethora of hydrazine and arylamine drugs, and is able to bioactivate several known carcinogens. KEYWORDS: 3D-structure;Acyltransferase;Cytoplasm;Direct protein sequencing;Reference proteome;Transferase SUBCELLULAR LOCATION: Cytoplasm. " P11279,"PROTEIN NAMES: Lysosome-associated membrane glycoprotein 1 (LAMP-1) (Lysosome-associated membrane protein 1) (CD107 antigen-like family member A) (CD antigen CD107a) PROTEIN FAMILY: LAMP family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the LAMP family. FUNCTION: Lysosomal membrane glycoprotein which plays an important role in lysosome biogenesis, lysosomal pH regulation, autophagy and cholesterol homeostasis. Acts as an important regulator of lysosomal lumen pH regulation by acting as a direct inhibitor of the proton channel TMEM175, facilitating lysosomal acidification for optimal hydrolase activity. Also plays an important role in NK-cells cytotoxicity. Mechanistically, participates in cytotoxic granule movement to the cell surface and perforin trafficking to the lytic granule. In addition, protects NK-cells from degranulation-associated damage induced by their own cytotoxic granule content. Presents carbohydrate ligands to selectins.; FUNCTION: (Microbial infection) Acts as a receptor for Lassa virus glycoprotein. Promotes also fusion of the virus with host membrane in less acidic endosomes.; FUNCTION: (Microbial infection) Supports the FURIN-mediated cleavage of mumps virus fusion protein F by interacting with both FURIN and the unprocessed form but not the processed form of the viral protein F. KEYWORDS: 3D-structure;Alternative splicing;Cell membrane;Direct protein sequencing;Disulfide bond;Endosome;Glycoprotein;Host cell receptor for virus entry;Host-virus interaction;Lysosome;Membrane;Receptor;Reference proteome;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Lysosome membrane ; Single-pass type I membrane protein Endosome membrane ; Single-pass type I membrane protein Late endosome membrane ; Single-pass type I membrane protein Cell membrane ; Single-pass type I membrane protein Cytolytic granule membrane ; Single-pass type I membrane protein Note=This protein shuttles between lysosomes, endosomes, and the plasma membrane (By similarity). Colocalizes with OSBPL1A at the late endosome. " P11411,PROTEIN NAMES: Glucose-6-phosphate 1-dehydrogenase (G6PD) (Glucose-6-phosphate dehydrogenase (NAD(P)(+))) PROTEIN FAMILY: Glucose-6-phosphate dehydrogenase family ORGANISM: Leuconostoc mesenteroides SIMILARITY: Belongs to the glucose-6-phosphate dehydrogenase family. FUNCTION: Catalyzes the oxidation of glucose 6-phosphate to 6-phosphogluconolactone. Can utilize either NADP(+) or NAD(+). PATHWAY: Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3. KEYWORDS: 3D-structure;Carbohydrate metabolism;Direct protein sequencing;Glucose metabolism;NAD;NADP;Oxidoreductase P11461,PROTEIN NAMES: Ferric-anguibactin receptor FatA (OM2) (Outer-membrane receptor FatA) PROTEIN FAMILY: TonB-dependent receptor family ORGANISM: Vibrio anguillarum (strain ATCC 68554 / 775) (Listonella anguillarum) SIMILARITY: Belongs to the TonB-dependent receptor family. FUNCTION: Involved in the uptake of iron in complex with the siderophore anguibactin. Binds and transports ferric-anguibactin from the cell surface to the periplasm. KEYWORDS: Cell outer membrane;Ion transport;Iron;Iron transport;Membrane;Plasmid;Receptor;Signal;TonB box;Transmembrane;Transmembrane beta strand;Transport SUBCELLULAR LOCATION: Cell outer membrane ; Multi-pass membrane protein P11491,"PROTEIN NAMES: Repressible alkaline phosphatase (Fructose-2,6-bisphosphate 6-phosphatase) (Membrane-bound repressible alkaline phosphatase) [Cleaved into: Soluble alkaline phosphatase (Farnesyl diphosphatase)] PROTEIN FAMILY: Alkaline phosphatase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the alkaline phosphatase family. FUNCTION: Phosphatase with broad substrate specificity. A truncated (soluble) version of the protein is responsible for the production of (E,E)-farnesol from (E,E)-farnesyl diphosphate. Acts as a fructose-2,6-bisphosphate 6-phosphatase. KEYWORDS: Cytoplasm;Direct protein sequencing;Glycoprotein;Hydrolase;Magnesium;Membrane;Metal-binding;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix;Vacuole;Zinc SUBCELLULAR LOCATION: [Repressible alkaline phosphatase]: Vacuole membrane; Single-pass membrane protein. Note=The full-length version is found in lysosome-like vacuoles.; SUBCELLULAR LOCATION: [Soluble alkaline phosphatase]: Cytoplasm. Note=The truncated version of the protein is soluble. MISCELLANEOUS: Present with 3060 molecules/cell in log phase SD medium." P11536,PROTEIN NAMES: Ecdysone-induced protein 74EF isoform B (ETS-related protein E74B) PROTEIN FAMILY: ETS family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the ETS family. KEYWORDS: Alternative splicing;Developmental protein;DNA-binding;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus. P11544,"PROTEIN NAMES: Phenylalanine/tyrosine ammonia-lyase (Bifunctional phenylalanine ammonia-lyase) (Bifunctional PAL) PROTEIN FAMILY: PAL/histidase family ORGANISM: Rhodotorula toruloides (Yeast) (Rhodosporidium toruloides) SIMILARITY: Belongs to the PAL/histidase family. FUNCTION: Catalyzes the non-oxidative deamination of L-phenylalanine and L-tyrosine to form trans-cinnamic acid and p-coumaric acid respectively with similar efficiencies. Facilitates the commitment step in phenylpropanoid pathways that produce secondary metabolites such as lignins, coumarins and flavonoids. PATHWAY: Phenylpropanoid metabolism; trans-cinnamate biosynthesis; trans-cinnamate from L-phenylalanine: step 1/1. KEYWORDS: 3D-structure;Cytoplasm;Lyase;Phenylalanine catabolism;Phenylpropanoid metabolism SUBCELLULAR LOCATION: Cytoplasm " P11557,PROTEIN NAMES: Cell division protein DamX PROTEIN FAMILY: DamX family ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the DamX family. FUNCTION: Non-essential cell division protein. KEYWORDS: 3D-structure;Cell cycle;Cell division;Cell inner membrane;Cell membrane;Coiled coil;Membrane;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell inner membrane ; Single-pass membrane protein Note=Localizes at the septal ring. Recruitment to the septal ring requires FtsZ. MISCELLANEOUS: Overexpression induces cell filamentation. P11584,"PROTEIN NAMES: Integrin beta-PS (Position-specific antigen beta subunit) (Protein myospheroid) (Protein olfactory C) PROTEIN FAMILY: Integrin beta chain family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the integrin beta chain family. FUNCTION: Integrin alpha-PS1/beta-PS is a receptor for laminin. Integrin alpha-PS2/beta-PS is a receptor for Tig, wb and Ten-m. Contributes to endodermal integrity and adhesion between the midgut epithelium and the surrounding visceral muscle. Essential for migration of the primordial midgut cells and for maintaining, but not establishing, cell polarity in the midgut epithelium. The two beta subunits mediate midgut migration by distinct mechanisms: beta-PS requires rhea/talin and Itgbn does not. Required for rhea/talin correct cellular localization in the midgut. Required for many embryonic (dorsal closure and somatic muscle attachments) and postembryonic developmental processes (attachment between cell layers of imaginal disks, organization of ommatidial arrays and flight muscle development). Involved in the function and/or development of the olfactory system. In the testes, essential for shv-dependent maintenance of somatic hub cells and their localization to the apical tip. Plays a role in timely border cell migration during oogenesis. KEYWORDS: Behavior;Cell adhesion;Cell membrane;Developmental protein;Disulfide bond;Flight;Glycoprotein;Integrin;Membrane;Olfaction;Phosphoprotein;Receptor;Reference proteome;Repeat;Sensory transduction;Signal;Transmembrane;Transmembrane helix;Vision SUBCELLULAR LOCATION: Apical cell membrane ; Single-pass type I membrane protein Lateral cell membrane ; Single-pass type I membrane protein Basal cell membrane ; Single-pass type I membrane protein Note=In ovary, localizes to the apical, lateral and basal membranes of follicle cells through oogenesis stage 10A. Apical membrane expression peaks at oogenesis stages 9 and 10A in columnar follicle cells overlying the oocyte but decreases in the most posterior follicle cells. Thereafter, it is down-regulated. Localization to lateral and basal membranes persists during dorsal appendage morphogenesis. MISCELLANEOUS: The absence of the beta-PS subunit results in detachment and rounding up of the muscles, thus the gene encoding beta-PS is called myospheroid." P11598,"PROTEIN NAMES: Protein disulfide-isomerase A3 (58 kDa glucose-regulated protein) (58 kDa microsomal protein) (p58) (Disulfide isomerase ER-60) (Endoplasmic reticulum resident protein 57) (ER protein 57) (ERp57) (Endoplasmic reticulum resident protein 60) (ER protein 60) (ERp60) (HIP-70) (Q-2) PROTEIN FAMILY: Protein disulfide isomerase family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the protein disulfide isomerase family. FUNCTION: Protein disulfide isomerase that catalyzes the formation, isomerization, and reduction or oxidation of disulfide bonds in client proteins and functions as a protein folding chaperone. Core component of the major histocompatibility complex class I (MHC I) peptide loading complex where it functions as an essential folding chaperone for TAPBP. Through TAPBP, assists the dynamic assembly of the MHC I complex with high affinity antigens in the endoplasmic reticulum. Therefore, plays a crucial role in the presentation of antigens to cytotoxic T cells in adaptive immunity. KEYWORDS: Acetylation;Adaptive immunity;Direct protein sequencing;Disulfide bond;Endoplasmic reticulum;Immunity;Isomerase;Methylation;Phosphoprotein;Redox-active center;Reference proteome;Repeat;Signal SUBCELLULAR LOCATION: Endoplasmic reticulum Endoplasmic reticulum lumen Melanosome " P11710,PROTEIN NAMES: Nuclear fusion protein FUS1 ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Required for cell fusion. Negatively regulates Sho1p signaling to ensure efficient cell fusion.; FUNCTION: Interacts with SHO1. KEYWORDS: Glycoprotein;Membrane;Phosphoprotein;Reference proteome;SH3 domain;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Single-pass membrane protein P11745,"PROTEIN NAMES: Ran GTPase-activating protein 1 (Protein involved in RNA production/processing) PROTEIN FAMILY: RNA1 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the RNA1 family. FUNCTION: GTPase activator for the nuclear Ras-related regulatory protein GSP1 (Ran), converting it to the putatively inactive GDP-bound state. KEYWORDS: Cytoplasm;GTPase activation;Leucine-rich repeat;Phosphoprotein;Reference proteome;Repeat SUBCELLULAR LOCATION: Cytoplasm. MISCELLANEOUS: Present with 52200 molecules/cell in log phase SD medium." P11746,"PROTEIN NAMES: Pheromone receptor transcription factor (GRM/PRTF protein) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Transcription factor required for the efficient replication of minichromosomes and the transcriptional regulation of early cell cycle genes. Activates transcription of ECB-dependent genes during the G1/M phase. Genes that contain a ECB (early cell box) element in their transcription regulatory region are transcribed only during G1/M phases. Interacts with the alpha-2 repressor or with the alpha-1 activator thereby regulating the expression of mating-type-specific genes. With ARG80, ARG81 and ARG82, coordinates the expression of arginine anabolic and catabolic genes in response to arginine. KEYWORDS: 3D-structure;Acetylation;Activator;Arginine metabolism;Cell cycle;DNA-binding;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus. MISCELLANEOUS: Present with 8970 molecules/cell in log phase SD medium." P11914,"PROTEIN NAMES: Mitochondrial-processing peptidase subunit alpha (Alpha-MPP) (Inactive zinc metalloprotease alpha) (Matrix processing peptidase) (MPP) (Mitochondrial assembly protein 2) (Mitochondrial import function protein 2) PROTEIN FAMILY: Peptidase M16 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the peptidase M16 family. FUNCTION: Substrate recognition and binding subunit of the essential mitochondrial processing protease (MPP), which cleaves the mitochondrial sequence off newly imported precursors proteins. KEYWORDS: 3D-structure;Direct protein sequencing;Mitochondrion;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Mitochondrion matrix MISCELLANEOUS: Present with 31400 molecules/cell in log phase SD medium." P11972,PROTEIN NAMES: Protein SST2 ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Desensitization to alpha-factor pheromone. Is involved in regulating the signaling pathway for responding to mating pheromone. KEYWORDS: Pheromone response;Phosphoprotein;Reference proteome;Signal transduction inhibitor MISCELLANEOUS: Present with 5980 molecules/cell in log phase SD medium. P12063,"PROTEIN NAMES: Tyrosine--tRNA ligase, mitochondrial (Tyrosyl-tRNA synthetase) (TyrRS) PROTEIN FAMILY: Class-I aminoacyl-tRNA synthetase family ORGANISM: Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family. FUNCTION: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr). Has both an aminoacyl-tRNA synthetase activity and is involved in the splicing of group I introns. It acts in intron splicing by stabilizing the catalytically active structure of the intron. KEYWORDS: 3D-structure;Aminoacyl-tRNA synthetase;ATP-binding;Ligase;Mitochondrion;mRNA processing;Nucleotide-binding;Protein biosynthesis;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Mitochondrion matrix. " P12294,"PROTEIN NAMES: Endonuclease SceI small subunit (Endo.SceI 50 kDa subunit) (Maturase-like RF3 protein) PROTEIN FAMILY: LAGLIDADG endonuclease family ORGANISM: Saccharomyces cerevisiae (Baker's yeast) SIMILARITY: Belongs to the LAGLIDADG endonuclease family. FUNCTION: Catalytic component of endonuclease SceI (Endo.SceI), which cleaves specifically at multiple sites on mitochondrial DNA and produces double-stranded breaks. KEYWORDS: Direct protein sequencing;Endonuclease;Hydrolase;Mitochondrion;Nuclease SUBCELLULAR LOCATION: Mitochondrion. " P12428,"PROTEIN NAMES: Protein brown (ATP-binding cassette transporter sub-family G member brown) (Broad substrate specificity ATP-binding cassette transporter brown) PROTEIN FAMILY: ABC transporter superfamily, ABCG family, Eye pigment precursor importer subfamily ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily. FUNCTION: ATP-dependent transporter of the ATP-binding cassette (ABC) family which transports various molecules including bioamines, neurotransmitters and metabolic intermediates. In the eye and probably in association with w/white, required for the transport of the eye red pigment precursor, guanine, into pigment cell granules. In Malpighian tubules, involved in guanine uptake. Probably in association with w/white, involved in aging-induced intestinal stem cell proliferation in the midgut by regulating tetrahydrofolate transport. KEYWORDS: ATP-binding;Membrane;Nucleotide-binding;Pigment;Reference proteome;Translocase;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Membrane ; Multi-pass membrane protein " P12611,"PROTEIN NAMES: Growth regulation protein PROTEIN FAMILY: WHI2 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the WHI2 family. FUNCTION: Plays a role in the coordination of growth and proliferation. Required for entry into G0 phase under conditions of carbon limitation. Involved in the general stress response; acts together with PSR1 to activate stress response element (STRE)-mediated gene expression, possibly through dephosphorylation of MSN2. KEYWORDS: Cell cycle;Reference proteome MISCELLANEOUS: Present with 1800 molecules/cell in log phase SD medium." P12689,PROTEIN NAMES: DNA repair protein REV1 (Reversionless protein 1) PROTEIN FAMILY: DNA polymerase type-Y family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the DNA polymerase type-Y family. FUNCTION: Deoxycytidyl transferase involved in DNA repair. Transfers a dCMP residue from dCTP to the 3'-end of a DNA primer in a template-dependent reaction. May assist in the first step in the bypass of abasic lesions by the insertion of a nucleotide opposite the lesion. Required for normal induction of mutations by physical and chemical agents. Involved in mitochondrial DNA mutagenesis. KEYWORDS: 3D-structure;DNA damage;DNA repair;DNA synthesis;DNA-binding;Magnesium;Metal-binding;Mitochondrion;Nucleotidyltransferase;Nucleus;Reference proteome;Transferase SUBCELLULAR LOCATION: Nucleus. Mitochondrion. MISCELLANEOUS: Present with 521 molecules/cell in log phase SD medium. P12695,"PROTEIN NAMES: Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial (Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex) (Pyruvate dehydrogenase complex component E2) (PDC-E2) (PDCE2) PROTEIN FAMILY: 2-oxoacid dehydrogenase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the 2-oxoacid dehydrogenase family. FUNCTION: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). KEYWORDS: Acyltransferase;Direct protein sequencing;Lipoyl;Mitochondrion;Reference proteome;Transferase;Transit peptide SUBCELLULAR LOCATION: Mitochondrion matrix MISCELLANEOUS: The E2 component contains covalently-bound lipoyl cofactors and it participates in the generation of acetyl groups from hydroxyethyl-thiamine pyrophosphate-E1 and their transfer to coenzyme A.; MISCELLANEOUS: Present with 5440 molecules/cell in log phase SD medium." P12754,"PROTEIN NAMES: Translation initiation factor eIF2B subunit delta (GCD complex subunit GCD2) (Guanine nucleotide exchange factor subunit GCD2) (eIF2B GDP-GTP exchange factor subunit delta) PROTEIN FAMILY: EIF-2B alpha/beta/delta subunits family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the eIF-2B alpha/beta/delta subunits family. FUNCTION: Acts as a component of the translation initiation factor 2B (eIF2B) complex, which catalyzes the exchange of GDP for GTP on the eukaryotic initiation factor 2 (eIF2) complex gamma subunit. Its guanine nucleotide exchange factor activity is repressed when bound to eIF2 complex phosphorylated on the alpha subunit, thereby limiting the amount of methionyl-initiator methionine tRNA available to the ribosome and consequently global translation is repressed (By similarity). It activates the synthesis of GCN4 in yeast under amino acid starvation conditions by suppressing the inhibitory effects of multiple AUG codons present in the leader of GCN4 mRNA. It may promote either repression or activation of GCN4 expression depending on amino acid availability. GCD2 is also required for cell viability. Its function can partially be replaced by GCN3 under normal growth conditions in GCD2-defective mutants, under AA starvation conditions GCN3 is an antagonist (GCN4 translational activator). KEYWORDS: 3D-structure;Acetylation;Cytoplasm;Initiation factor;Phosphoprotein;Protein biosynthesis;Reference proteome;Repressor;Translation regulation SUBCELLULAR LOCATION: Cytoplasm, cytosol MISCELLANEOUS: Present with 10300 molecules/cell in log phase SD medium." P12757,PROTEIN NAMES: Ski-like protein (Ski-related oncogene) (Ski-related protein) PROTEIN FAMILY: SKI family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the SKI family. FUNCTION: May have regulatory role in cell division or differentiation in response to extracellular signals. KEYWORDS: 3D-structure;Alternative splicing;Coiled coil;Isopeptide bond;Phosphoprotein;Reference proteome;Ubl conjugation P12928,"PROTEIN NAMES: Pyruvate kinase PKLR (L-PK) (Pyruvate kinase isozymes L/R) PROTEIN FAMILY: Pyruvate kinase family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the pyruvate kinase family. FUNCTION: Pyruvate kinase that catalyzes the conversion of phosphoenolpyruvate to pyruvate with the synthesis of ATP, and which plays a key role in glycolysis. PATHWAY: Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5. KEYWORDS: 3D-structure;Allosteric enzyme;Alternative splicing;ATP-binding;Glycolysis;Kinase;Magnesium;Manganese;Metal-binding;Nucleotide-binding;Phosphoprotein;Potassium;Pyruvate;Reference proteome;Transferase MISCELLANEOUS: There are 4 isozymes of pyruvate kinase in mammals: L, R, M1 and M2. L type is major isozyme in the liver, R is found in red cells, M1 is the main form in muscle, heart and brain, and M2 is found in early fetal tissues." P13051,"PROTEIN NAMES: Uracil-DNA glycosylase (UDG) PROTEIN FAMILY: Uracil-DNA glycosylase (UDG) superfamily, UNG family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the uracil-DNA glycosylase (UDG) superfamily. UNG family. FUNCTION: Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine. KEYWORDS: 3D-structure;Acetylation;Alternative splicing;Direct protein sequencing;Disease variant;DNA damage;DNA repair;Host-virus interaction;Hydrolase;Mitochondrion;Nucleus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: [Isoform 1]: Mitochondrion.; SUBCELLULAR LOCATION: [Isoform 2]: Nucleus. " P13203,"PROTEIN NAMES: Glucose 1-dehydrogenase (GDH) (GlcDH) (Galactose 1-dehydrogenase) PROTEIN FAMILY: Zinc-containing alcohol dehydrogenase family, Glucose 1-dehydrogenase subfamily ORGANISM: Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165) SIMILARITY: Belongs to the zinc-containing alcohol dehydrogenase family. Glucose 1-dehydrogenase subfamily. FUNCTION: Catalyzes the NAD(P)(+)-dependent oxidation of D-glucose to D-gluconate via gluconolactone. Is also significantly active with galactose as substrate, but not with mannose or glucose 6-phosphate. Can utilize both NAD(+) and NADP(+) as electron acceptor, with a marked preference for NADP(+). Physiologically, may be involved in the degradation of both glucose and galactose through a non-phosphorylative variant of the Entner-Doudoroff pathway. KEYWORDS: Carbohydrate metabolism;Direct protein sequencing;Metal-binding;NAD;NADP;Nucleotide-binding;Oxidoreductase;Reference proteome;Zinc " P13208,"PROTEIN NAMES: Sarafotoxin [Cleaved into: Sarafotoxin-A, Ser-isoform (SRTX-A) (Sarafotoxin-A) (S6A); Sarafotoxin-C (SRTX-C) (S6C); Sarafotoxin-B (SRTX-B) (S6B); Sarafotoxin-E (SRTX-E) (S6E); Sarafotoxin-A, Thr-isoform] PROTEIN FAMILY: Endothelin/sarafotoxin family ORGANISM: Atractaspis engaddensis (Israeli burrowing asp) (Israeli mole viper) SIMILARITY: Belongs to the endothelin/sarafotoxin family. FUNCTION: Vasoconstrictor activity. These toxins cause cardiac arrest probably as a result of coronary vasospasm.; FUNCTION: [Sarafotoxin-B]: Vasoconstrictor activity. Causes cardiac arrest probably as a result of coronary vasospasm (By similarity). Displays high agonistic activities towards endothelin-2 receptor (EDNRB) (displays affinity in the picomolar range) and endothelin-1 receptor (EDNRA) (lower affinities). KEYWORDS: 3D-structure;Cardiotoxin;Direct protein sequencing;Disulfide bond;G-protein coupled receptor impairing toxin;Repeat;Secreted;Signal;Toxin;Vasoactive;Vasoconstrictor SUBCELLULAR LOCATION: Secreted " P13284,PROTEIN NAMES: Gamma-interferon-inducible lysosomal thiol reductase (Gamma-interferon-inducible protein IP-30) (Legumaturain) PROTEIN FAMILY: GILT family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the GILT family. FUNCTION: Lysosomal thiol reductase that can reduce protein disulfide bonds. May facilitate the complete unfolding of proteins destined for lysosomal degradation. Plays an important role in antigen processing. Facilitates the generation of MHC class II-restricted epitodes from disulfide bond-containing antigen by the endocytic reduction of disulfide bonds (By similarity). Facilitates also MHC class I-restricted recognition of exogenous antigens containing disulfide bonds by CD8+ T-cells or crosspresentation (By similarity). KEYWORDS: Direct protein sequencing;Disulfide bond;Glycoprotein;Immunity;Lysosome;Oxidoreductase;Redox-active center;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted Lysosome MISCELLANEOUS: Both precursor form and mature form have thiol reductase activity. P13369,"PROTEIN NAMES: Macrophage colony-stimulating factor 1 receptor (CSF-1 receptor) (CSF-1-R) (CSF-1R) (M-CSF-R) (Proto-oncogene c-Fms) (CD antigen CD115) PROTEIN FAMILY: Protein kinase superfamily, Tyr protein kinase family, CSF-1/PDGF receptor subfamily ORGANISM: Felis catus (Cat) (Felis silvestris catus) SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily. FUNCTION: Tyrosine-protein kinase that acts as a cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of pro-inflammatory chemokines in response to IL34 and CSF1, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone and tooth development. Required for normal male and female fertility, and for normal development of milk ducts and acinar structures in the mammary gland during pregnancy. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration, and promotes cancer cell invasion. Activates several signaling pathways in response to ligand binding, including the ERK1/2 and the JNK pathway (By similarity). Phosphorylates PIK3R1, PLCG2, GRB2, SLA2 and CBL. Activation of PLCG2 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, that then lead to the activation of protein kinase C family members, especially PRKCD. Phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leads to activation of the AKT1 signaling pathway. Activated CSF1R also mediates activation of the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1, and of the SRC family kinases SRC, FYN and YES1. Activated CSF1R transmits signals both via proteins that directly interact with phosphorylated tyrosine residues in its intracellular domain, or via adapter proteins, such as GRB2. Promotes activation of STAT family members STAT3, STAT5A and/or STAT5B. Promotes tyrosine phosphorylation of SHC1 and INPP5D/SHIP-1. Receptor signaling is down-regulated by protein phosphatases, such as INPP5D/SHIP-1, that dephosphorylate the receptor and its downstream effectors, and by rapid internalization of the activated receptor (By similarity). In the central nervous system, may play a role in the development of microglia macrophages (By similarity). KEYWORDS: ATP-binding;Cell membrane;Disulfide bond;Glycoprotein;Immunity;Immunoglobulin domain;Inflammatory response;Innate immunity;Kinase;Membrane;Nucleotide-binding;Phosphoprotein;Proto-oncogene;Receptor;Reference proteome;Repeat;Signal;Transferase;Transmembrane;Transmembrane helix;Tyrosine-protein kinase;Ubl conjugation SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. Note=The autophosphorylated receptor is ubiquitinated and internalized, leading to its degradation. " P13512,"PROTEIN NAMES: Metal cation efflux system protein CzcD (Cobalt-zinc-cadmium resistance protein CzcD) PROTEIN FAMILY: Cation diffusion facilitator (CDF) transporter family, SLC30A subfamily ORGANISM: Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34) (Ralstonia metallidurans) SIMILARITY: Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. SLC30A subfamily. FUNCTION: Mediates a low-level metal ion resistance, probably by efflux of cations from the cytoplasm into the periplasm. Also mediates resistance to cobalt, cadmium and zinc via regulation of the Czc system. May repress expression of the Czc system by an export of the inducing cations. Binds and transports zinc. Can also bind cobalt, copper and nickel. KEYWORDS: 3D-structure;Cadmium;Cadmium resistance;Cell inner membrane;Cell membrane;Cobalt;Ion transport;Membrane;Plasmid;Reference proteome;Transmembrane;Transmembrane helix;Transport;Zinc;Zinc transport SUBCELLULAR LOCATION: Cell inner membrane ; Multi-pass membrane protein " P13518,"PROTEIN NAMES: RNase E specificity factor CsrD (Regulator of CsrB and CsrC decay CsrD) ORGANISM: Escherichia coli (strain K12) FUNCTION: Serves as a specificity factor required for RNase E-mediated decay of the small global regulatory RNAs CsrB and CsrC, it is probably not a nuclease. Nor does its activity involve c-di-GMP, despite its domain composition. Positively modulates motility gene expression, is also required for curli expression. KEYWORDS: Cell membrane;Coiled coil;Membrane;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein " P13574,"PROTEIN NAMES: Protein STE12 PROTEIN FAMILY: STE12 transcription factor family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the STE12 transcription factor family. FUNCTION: Binds to the DNA sequence mediating pheromone induction (called the pheromone response element = PRE) which is found in the upstream control region of several a-, alpha- and haploid-specific genes. Involved in mating of haploids and in pseudohyphae formation in diploids. KEYWORDS: 3D-structure;Activator;DNA-binding;Nucleus;Pheromone response;Phosphoprotein;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus. MISCELLANEOUS: Present with 1920 molecules/cell in log phase SD medium." P13658,"PROTEIN NAMES: DNA relaxase MbeA (DNA nickase) (Mobilization protein MbeA) ORGANISM: Escherichia coli SIMILARITY: To E.coli MbaA and MbkA. FUNCTION: Relaxase involved in plasmid ColE1 conjugative mobilization and is thus essential to promote the specific transfer of the plasmid during conjugation. First catalyzes the specific cleavage of one of the DNA strands at oriT, forming a covalent 5'-phosphotyrosine intermediate. The nic site corresponds to 5'-(1469)CTGG/CTTA(1462)-3' in the cleaved strand. The cleaved strand is then transferred through the dedicated type IV secretion apparatus. MbeA remains covalently linked at the 5' end of the strand, and once in the recipient cell, it probably catalyzes the rejoining of the two ends of the strand, re-forming the circular plasmid DNA. Is functional in vitro without a requirement for the conjugative accessory proteins. KEYWORDS: Cobalt;Conjugation;DNA-binding;Isomerase;Magnesium;Metal-binding;Mobility protein;Nickel;Plasmid;Topoisomerase MISCELLANEOUS: The 100 C-terminal amino acids are dispensable for activity." P13667,PROTEIN NAMES: Protein disulfide-isomerase A4 (Endoplasmic reticulum resident protein 70) (ER protein 70) (ERp70) (Endoplasmic reticulum resident protein 72) (ER protein 72) (ERp-72) (ERp72) PROTEIN FAMILY: Protein disulfide isomerase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the protein disulfide isomerase family. KEYWORDS: 3D-structure;Acetylation;Direct protein sequencing;Disulfide bond;Endoplasmic reticulum;Isomerase;Redox-active center;Reference proteome;Repeat;Signal SUBCELLULAR LOCATION: Endoplasmic reticulum lumen Melanosome Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. P13682,PROTEIN NAMES: Zinc finger protein 35 (Zinc finger protein HF.10) PROTEIN FAMILY: Krueppel C2H2-type zinc-finger protein family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein family. FUNCTION: May be involved in transcriptional regulation. Involved in cell differentiation and/or proliferation. KEYWORDS: DNA-binding;Isopeptide bond;Metal-binding;Nucleus;Reference proteome;Repeat;Transcription;Transcription regulation;Ubl conjugation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus P13717,"PROTEIN NAMES: Nuclease (Endonuclease) [Cleaved into: Nuclease isoform Sm2; Nuclease isoform Sm3; Nuclease isoform Sm1] PROTEIN FAMILY: DNA/RNA non-specific endonuclease family ORGANISM: Serratia marcescens SIMILARITY: Belongs to the DNA/RNA non-specific endonuclease family. FUNCTION: Catalyzes the hydrolysis of both DNA and RNA, double- or single-stranded, at the 3'position of the phosphodiester bond to produce 5'-phosphorylated mono-, di-, tri- and tetranucleotides. DNA is a slightly better substrate than RNA. KEYWORDS: 3D-structure;Direct protein sequencing;Disulfide bond;Endonuclease;Hydrolase;Magnesium;Metal-binding;Nuclease;Secreted;Signal SUBCELLULAR LOCATION: Secreted. MISCELLANEOUS: The active site contains 1 hydrated magnesium ion that has only 1 direct interaction with the protein; all other interactions are via water molecules." P13726,PROTEIN NAMES: Tissue factor (TF) (Coagulation factor III) (Thromboplastin) (CD antigen CD142) PROTEIN FAMILY: Tissue factor family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the tissue factor family. FUNCTION: Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited proteolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade. KEYWORDS: 3D-structure;Alternative splicing;Blood coagulation;Disulfide bond;Glycoprotein;Hemostasis;Lipoprotein;Membrane;Palmitate;Reference proteome;Secreted;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: [Isoform 1]: Membrane ; Single-pass type I membrane protein.; SUBCELLULAR LOCATION: [Isoform 2]: Secreted P13801,PROTEIN NAMES: Sporulation sigma-E factor-processing peptidase (Membrane-associated aspartic protease) (Stage II sporulation protein GA) PROTEIN FAMILY: Peptidase U4 family ORGANISM: Bacillus subtilis (strain 168) SIMILARITY: Belongs to the peptidase U4 family. FUNCTION: Probable aspartic protease that is responsible for the proteolytic cleavage of the RNA polymerase sigma E factor (SigE/spoIIGB) to yield the active peptide in the mother cell during sporulation. Responds to a signal from the forespore that is triggered by the extracellular signal protein SpoIIR. KEYWORDS: Aspartyl protease;Cell membrane;Hydrolase;Membrane;Protease;Reference proteome;Sporulation;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Note=Localized to the sporulation septum. P13995,"PROTEIN NAMES: Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial [Includes: NAD-dependent methylenetetrahydrofolate dehydrogenase ; Methenyltetrahydrofolate cyclohydrolase ] PROTEIN FAMILY: Tetrahydrofolate dehydrogenase/cyclohydrolase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family. FUNCTION: Although its dehydrogenase activity is NAD-specific, it can also utilize NADP at a reduced efficiency. KEYWORDS: 3D-structure;Acetylation;Alternative splicing;Hydrolase;Isopeptide bond;Magnesium;Mitochondrion;Multifunctional enzyme;NAD;NADP;One-carbon metabolism;Oxidoreductase;Reference proteome;Transit peptide;Ubl conjugation SUBCELLULAR LOCATION: Mitochondrion. MISCELLANEOUS: This NAD-dependent bifunctional enzyme has very different kinetic properties than the larger NADP-dependent trifunctional enzyme and is unique in that it requires formation of an enzyme-magnesium complex to allow binding of NAD." P14081,"PROTEIN NAMES: Selenocysteine-specific elongation factor (SelB translation factor) PROTEIN FAMILY: TRAFAC class translation factor GTPase superfamily, Classic translation factor GTPase family, SelB subfamily ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. SelB subfamily. FUNCTION: Translation factor necessary for the incorporation of selenocysteine into proteins. It probably replaces EF-Tu for the insertion of selenocysteine directed by the UGA codon. SelB binds GTP and GDP. KEYWORDS: 3D-structure;Cytoplasm;GTP-binding;Nucleotide-binding;Protein biosynthesis;Reference proteome SUBCELLULAR LOCATION: Cytoplasm. MISCELLANEOUS: There are about 1100 copies of SelB per E.coli cell." P14138,PROTEIN NAMES: Endothelin-3 (ET-3) (Preproendothelin-3) (PPET3) PROTEIN FAMILY: Endothelin/sarafotoxin family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the endothelin/sarafotoxin family. FUNCTION: Endothelins are endothelium-derived vasoconstrictor peptides. KEYWORDS: 3D-structure;Alternative splicing;Cleavage on pair of basic residues;Deafness;Direct protein sequencing;Disease variant;Disulfide bond;Hirschsprung disease;Reference proteome;Secreted;Signal;Vasoactive;Vasoconstrictor;Waardenburg syndrome SUBCELLULAR LOCATION: Secreted. P14180,"PROTEIN NAMES: Chitin synthase 2 (Chitin-UDP acetyl-glucosaminyl transferase 2) PROTEIN FAMILY: Chitin synthase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the chitin synthase family. FUNCTION: Polymerizes chitin, a structural polymer of the cell wall and septum, by transferring the sugar moiety of UDP-GlcNAc to the non-reducing end of the growing chitin polymer (Probable). Required for septum formation (Probable). KEYWORDS: Cell wall biogenesis/degradation;Glycoprotein;Glycosyltransferase;Membrane;Phosphoprotein;Reference proteome;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Multi-pass membrane protein " P14222,"PROTEIN NAMES: Perforin-1 (P1) (Cytolysin) (Lymphocyte pore-forming protein) (PFP) PROTEIN FAMILY: Complement C6/C7/C8/C9 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the complement C6/C7/C8/C9 family. FUNCTION: Pore-forming protein that plays a key role in granzyme-mediated programmed cell death, and in defense against virus-infected or neoplastic cells. Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by mediating the passage and uptake of cytotoxic granzymes. Facilitates the delivery of cationic cargo protein, while anionic or neural proteins are not delivered efficiently. Perforin pores allow the release of mature caspase-7 (CASP7) into the extracellular milieu (By similarity). KEYWORDS: Calcium;Cell membrane;Cytolysis;Disease variant;Disulfide bond;EGF-like domain;Endosome;Familial hemophagocytic lymphohistiocytosis;Glycoprotein;Lysosome;Membrane;Metal-binding;Reference proteome;Secreted;Signal;Transmembrane;Transmembrane beta strand SUBCELLULAR LOCATION: Cytolytic granule Secreted. Cell membrane ; Multi-pass membrane protein Endosome lumen Note=Stored in cytolytic granules of cytolytic T-lymphocytes and secreted into the cleft between T-lymphocyte and target cell. Inserts into the cell membrane of target cells and forms pores. Membrane insertion and pore formation requires a major conformation change. May be taken up via endocytosis involving clathrin-coated vesicles and accumulate in a first time in large early endosomes. " P14324,"PROTEIN NAMES: Farnesyl pyrophosphate synthase (FPP synthase) (FPS) ((2E,6E)-farnesyl diphosphate synthase) (Dimethylallyltranstransferase) (Farnesyl diphosphate synthase) (Geranyltranstransferase) PROTEIN FAMILY: FPP/GGPP synthase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the FPP/GGPP synthase family. FUNCTION: Key enzyme in isoprenoid biosynthesis which catalyzes the formation of farnesyl diphosphate (FPP), a precursor for several classes of essential metabolites including sterols, dolichols, carotenoids, and ubiquinones. FPP also serves as substrate for protein farnesylation and geranylgeranylation. Catalyzes the sequential condensation of isopentenyl pyrophosphate with the allylic pyrophosphates, dimethylallyl pyrophosphate, and then with the resultant geranylpyrophosphate to the ultimate product farnesyl pyrophosphate. PATHWAY: Isoprenoid biosynthesis; farnesyl diphosphate biosynthesis; farnesyl diphosphate from geranyl diphosphate and isopentenyl diphosphate: step 1/1.; PATHWAY: Isoprenoid biosynthesis; geranyl diphosphate biosynthesis; geranyl diphosphate from dimethylallyl diphosphate and isopentenyl diphosphate: step 1/1. KEYWORDS: 3D-structure;Acetylation;Alternative splicing;Cholesterol biosynthesis;Cholesterol metabolism;Cytoplasm;Disease variant;Host-virus interaction;Hydroxylation;Isoprene biosynthesis;Lipid biosynthesis;Lipid metabolism;Magnesium;Metal-binding;Reference proteome;Steroid biosynthesis;Steroid metabolism;Sterol biosynthesis;Sterol metabolism;Transferase SUBCELLULAR LOCATION: Cytoplasm. " P14377,PROTEIN NAMES: Sensor protein ZraS ORGANISM: Escherichia coli (strain K12) FUNCTION: Member of the two-component regulatory system ZraS/ZraR. May function as a membrane-associated protein kinase that phosphorylates ZraR in response to high concentrations of zinc or lead in the medium. KEYWORDS: ATP-binding;Cell inner membrane;Cell membrane;Kinase;Membrane;Nucleotide-binding;Phosphoprotein;Reference proteome;Transferase;Transmembrane;Transmembrane helix;Two-component regulatory system;Zinc SUBCELLULAR LOCATION: Cell inner membrane ; Multi-pass membrane protein P14448,"PROTEIN NAMES: Fibrinogen alpha chain [Cleaved into: Fibrinopeptide A; Fibrinogen alpha chain] ORGANISM: Gallus gallus (Chicken) FUNCTION: Cleaved by the protease thrombin to yield monomers which, together with fibrinogen beta (FGB) and fibrinogen gamma (FGG), polymerize to form an insoluble fibrin matrix. Fibrin has a major function in hemostasis as one of the primary components of blood clots. KEYWORDS: 3D-structure;Alternative splicing;Blood coagulation;Calcium;Coiled coil;Direct protein sequencing;Disulfide bond;Hemostasis;Metal-binding;Pyrrolidone carboxylic acid;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted " P14532,PROTEIN NAMES: Cytochrome c551 peroxidase (CCP) (Cytochrome c peroxidase) ORGANISM: Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) FUNCTION: Catalyzes the peroxidative oxidation of azurin and cytochrome c551. Likely to provide protection against toxic peroxides. KEYWORDS: 3D-structure;Direct protein sequencing;Electron transport;Heme;Iron;Metal-binding;Oxidoreductase;Periplasm;Peroxidase;Reference proteome;Signal;Transport SUBCELLULAR LOCATION: Periplasm P14693,"PROTEIN NAMES: Sorting assembly machinery 35 kDa subunit (Mitochondrial 38 kDa outer membrane protein) (TOB complex 38 kDa subunit) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Essential component of the mitochondrial outer membrane sorting assembly machinery (SAM or TOB) complex, which is required for the sorting of proteins with complicated topology, such as beta-barrel proteins, to the mitochondrial outer membrane after import by the TOM complex. KEYWORDS: 3D-structure;Membrane;Mitochondrion;Mitochondrion outer membrane;Protein transport;Reference proteome;Transport SUBCELLULAR LOCATION: Mitochondrion outer membrane MISCELLANEOUS: Present with 1470 molecules/cell in log phase SD medium." P14743,"PROTEIN NAMES: Glycylpeptide N-tetradecanoyltransferase (Cell division control protein 72) (Myristoyl-CoA:protein N-myristoyltransferase) (NMT) (Peptide N-myristoyltransferase) PROTEIN FAMILY: NMT family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the NMT family. FUNCTION: Adds a myristoyl group to the N-terminal glycine residue of certain cellular proteins. Substrate specificity requires an N-terminal glycine in the nascent polypeptide substrates. Uncharged amino acids are preferred at position 2 while neutral residues are favored at positions 3 and 4. Ser is present at position 5 in almost all known N-myristoyl proteins and Lys is commonly encountered at postion 6. KEYWORDS: 3D-structure;Acyltransferase;Cytoplasm;Direct protein sequencing;Reference proteome;Transferase SUBCELLULAR LOCATION: Cytoplasm MISCELLANEOUS: Has an ordered Bi-Bi kinetic mechanism, with myristoyl-CoA binding taking place prior to peptide binding and CoA release occurring before acylated peptide release. Cooperative interactions between the acyl-CoA and peptide binding sites of NMT contribute to its extraordinary chain-length specificity." P14756,"PROTEIN NAMES: Elastase (Neutral metalloproteinase) (PAE) (Pseudolysin) [Cleaved into: Pro-elastase] PROTEIN FAMILY: Peptidase M4 family ORGANISM: Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) SIMILARITY: Belongs to the peptidase M4 family. FUNCTION: Cleaves host elastin, collagen, IgG, and several complement components as well as endogenous pro-aminopeptidase. Autocatalyses processing of its pro-peptide. Processes the pro-peptide of pro-chitin-binding protein (cbpD). Involved in the pathogenesis of P.aeruginosa infections. KEYWORDS: 3D-structure;Autocatalytic cleavage;Calcium;Direct protein sequencing;Disulfide bond;Hydrolase;Metal-binding;Metalloprotease;Protease;Reference proteome;Secreted;Signal;Virulence;Zinc;Zymogen SUBCELLULAR LOCATION: Secreted Note=Secreted in an Xcp-dependent fashion (a type II secretion pathway). " P14784,"PROTEIN NAMES: Interleukin-2 receptor subunit beta (IL-2 receptor subunit beta) (IL-2R subunit beta) (IL-2RB) (High affinity IL-2 receptor subunit beta) (Interleukin-15 receptor subunit beta) (p70-75) (p75) (CD antigen CD122) PROTEIN FAMILY: Type I cytokine receptor family, Type 4 subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the type I cytokine receptor family. Type 4 subfamily. FUNCTION: Receptor for interleukin-2. This beta subunit is involved in receptor mediated endocytosis and transduces the mitogenic signals of IL2. Probably in association with IL15RA, involved in the stimulation of neutrophil phagocytosis by IL15. KEYWORDS: 3D-structure;Cell membrane;Disease variant;Disulfide bond;Glycoprotein;Host-virus interaction;Membrane;Receptor;Reference proteome;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein " P14789,"PROTEIN NAMES: Protease LasA (Staphylolytic protease) PROTEIN FAMILY: Peptidase M23A family ORGANISM: Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) SIMILARITY: Belongs to the peptidase M23A family. FUNCTION: Involved in proteolysis and elastolysis (degradation of the host protein elastin). Has staphylolytic activity (degrades pentaglycine cross-links in cell wall peptidoglycan), preferring Gly-Gly-|-X substrates where X is Ala or Gly. Enhances the elastolytic but not proteolytic activity of elastase (lasB) and elastolytic activity of other proteases. Degradation of host elastin is likely to contribute to the pathogenicity of P.aeruginosa. While either His-317 or His-356 can abstract a proton in the hydrolysis reaction, the same residue performs both functions in a given catalytic cycle, with the other stabilizing the catalytic intermediate. KEYWORDS: 3D-structure;Direct protein sequencing;Disulfide bond;Hydrolase;Metal-binding;Metalloprotease;Protease;Reference proteome;Secreted;Signal;Virulence;Zinc;Zymogen SUBCELLULAR LOCATION: Secreted Note=Secreted in an Xcp-dependent fashion (a type II secretion pathway). " P14904,"PROTEIN NAMES: Vacuolar aminopeptidase 1 (Aminopeptidase yscI) (Leucine aminopeptidase IV) (LAPIV) (Lysosomal aminopeptidase III) (Polypeptidase) (Vacuolar aminopeptidase I) PROTEIN FAMILY: Peptidase M18 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the peptidase M18 family. FUNCTION: Resident vacuolar enzyme that catalyzes the removal of amino acids from the N-terminus of peptides and proteins. Also acts as the major cargo protein of the cytoplasm-to-vacuole targeting (Cvt) pathway. The precursor form of aminopeptidase 1 (prApe1) assembles into dodecamers and the propeptide mediates the aggregation of dodecamers into higher multimers. The multimers are then recognized via the propeptide by their receptor ATG19, and ATG19 further interacts with ATG11, which tethers the APE1-ATG19 complex to the pre-autophagosomal structure (PAS). The cargo-receptor complex (also Cvt complex) is selectively enwrapped by a double-membrane structure termed the Cvt vesicle under vegetative growth conditions and by a similar but larger double-membrane structure termed the autophagosome under nitrogen starvation conditions. The Cvt vesicle or the autophagosome fuses with the vacuolar membrane and release its content in the vacuolar lumen. In the vacuole, prApe1 is processed into mature aminopeptidase 1 (mApe1). KEYWORDS: 3D-structure;Aminopeptidase;Direct protein sequencing;Glycoprotein;Hydrolase;Metal-binding;Metalloprotease;Phosphoprotein;Protease;Protein transport;Reference proteome;Transport;Vacuole;Zinc;Zymogen SUBCELLULAR LOCATION: Vacuole Note=Transported to the vacuole by the cytosol-to-vacuole targeting (Cvt) pathway. MISCELLANEOUS: Present with 5730 molecules/cell in log phase SD medium." P14906,PROTEIN NAMES: Protein translocation protein SEC63 (Protein NPL1) (Sec62/63 complex 73 kDa subunit) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Acts as a component of the Sec62/63 complex which is involved in SRP-independent post-translational translocation across the endoplasmic reticulum (ER) and functions together with the Sec61 complex and KAR2 in a channel-forming translocon complex. A cycle of assembly and disassembly of Sec62/63 complex from SEC61 may govern the activity of the translocon. SEC63 may affect SEC1-polypeptide interactions by increasing the affinity of targeting pathways for SEC61 and/or by modifying SEC61 to allow more efficient polypeptide interaction. May also be involved in SRP-dependent cotranslational translocation. Is essential for cell growth and for germination. KEYWORDS: 3D-structure;Chaperone;Endoplasmic reticulum;Membrane;Nucleus;Phosphoprotein;Protein transport;Reference proteome;Repeat;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass membrane protein. Nucleus membrane; Multi-pass membrane protein. Nucleus inner membrane; Multi-pass membrane protein. MISCELLANEOUS: Present with 17700 molecules/cell in log phase SD medium. P14908,"PROTEIN NAMES: Mitochondrial transcription factor 1 (Mitochondrial transcription factor mtTFB) (Mitochondrial-specificity factor) (RF1023) PROTEIN FAMILY: Class I-like SAM-binding methyltransferase superfamily, rRNA adenine N(6)-methyltransferase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. FUNCTION: Mitochondrial transcription factor that confers selective promoter recognition on the core subunit of the yeast mitochondrial RNA polymerase. Interacts with DNA in a non-specific manner. KEYWORDS: 3D-structure;Direct protein sequencing;DNA-binding;Methyltransferase;Mitochondrion;Reference proteome;RNA-binding;S-adenosyl-L-methionine;Transcription;Transcription regulation;Transferase SUBCELLULAR LOCATION: Mitochondrion intermembrane space MISCELLANEOUS: Present with 9380 molecules/cell in log phase SD medium.; MISCELLANEOUS: Although strongly related to dimethyladenosine transferase proteins, it lacks the methyltransferase activity. Dimethyladenosine transferase methylates the 2 adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 12S mitochondrial rRNA in most species. This explains why 12S rRNA is not methylated in S.cerevisiae." P14930,PROTEIN NAMES: Peptide methionine sulfoxide reductase MsrA/MsrB [Includes: Thioredoxin; Peptide methionine sulfoxide reductase MsrA (Protein-methionine-S-oxide reductase) (Peptide-methionine (S)-S-oxide reductase) (Peptide Met(O) reductase); Peptide methionine sulfoxide reductase MsrB (Peptide-methionine (R)-S-oxide reductase)] PROTEIN FAMILY: Thioredoxin family; MsrA Met sulfoxide reductase family; MsrB Met sulfoxide reductase family ORGANISM: Neisseria gonorrhoeae SIMILARITY: In the N-terminal section; belongs to the thioredoxin family.; SIMILARITY: In the central section; belongs to the MsrA Met sulfoxide reductase family.; SIMILARITY: In the C-terminal section; belongs to the MsrB Met sulfoxide reductase family. FUNCTION: Has an important function as a repair enzyme for proteins that have been inactivated by oxidation (By similarity). Catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine. KEYWORDS: 3D-structure;Disulfide bond;Electron transport;Multifunctional enzyme;Oxidoreductase;Redox-active center;Transport P15047,"PROTEIN NAMES: 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase (DiDHB-DH) (Trans-2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase) PROTEIN FAMILY: Short-chain dehydrogenases/reductases (SDR) family ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FUNCTION: Involved in the biosynthesis of the siderophore enterobactin (enterochelin), which is a macrocyclic trimeric lactone of N-(2,3-dihydroxybenzoyl)-serine. Catalyzes the reversible NAD-dependent oxidation of the C3-hydroxyl group of 2,3-dihydro-2,3-dihydroxybenzoate (2,3-diDHB), producing the transient intermediate 2-hydroxy-3-oxo-4,6-cyclohexadiene-1-carboxylate, which undergoes rapid aromatization to the final product, 2,3-dihydroxybenzoate (2,3-DHB). Only the compounds with a C3-hydroxyl group such as methyl 2,3-dihydro-2,3-dihydroxybenzoate, methyl-3-hydroxy-1,4-cyclohexadiene-1-carboxylate, trans-3-hydroxy-2-cyclohexene-1-carboxylate, cis-3-hydroxy-4-cyclohexene-1-carboxylate, cis-3-hydroxycyclohexane-1-carboxylic acid are oxidized to the corresponding ketone products. The stereospecificity of the C3 allylic alcohol group oxidation is 3R in a 1R,3R dihydro substrate. It can also increase the DHB-AMP ligase activity of EntE by interaction EntE. PATHWAY: Siderophore biosynthesis; enterobactin biosynthesis. KEYWORDS: 3D-structure;Direct protein sequencing;Enterobactin biosynthesis;NAD;Oxidoreductase;Reference proteome " P15247,"PROTEIN NAMES: Interleukin-9 (IL-9) (Cytokine P40) (T-cell growth factor P40) PROTEIN FAMILY: IL-7/IL-9 family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the IL-7/IL-9 family. FUNCTION: Multifunctional cytokine secreted mainly by T-helper 2 lymphocytes and also mast cells or NKT cells that plays important roles in the immune response against parasites. Affects intestinal epithelial permeability and adaptive immunity. In addition, induces the differentiation of specific T-cell subsets such as IL-17 producing helper T-cells (TH17) and also proliferation and differentiation of mast cells. Mechanistically, exerts its biological effects through a receptor composed of IL9R subunit and a signal transducing subunit IL2RG. Receptor stimulation results in the rapid activation of JAK1 and JAK3 kinase activities leading to STAT1, STAT3 and STAT5-mediated transcriptional programs. Induction of differentiation genes seems to be mediated by STAT1 alone, while protection of cells from apoptosis depends on STAT3 and STAT5. KEYWORDS: 3D-structure;Cytokine;Direct protein sequencing;Glycoprotein;Growth factor;Pyrrolidone carboxylic acid;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted. " P15289,"PROTEIN NAMES: Arylsulfatase A (ASA) (Cerebroside-sulfatase) [Cleaved into: Arylsulfatase A component B; Arylsulfatase A component C] PROTEIN FAMILY: Sulfatase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the sulfatase family. FUNCTION: Hydrolyzes cerebroside sulfate. KEYWORDS: 3D-structure;Alternative splicing;Calcium;Direct protein sequencing;Disease variant;Disulfide bond;Endoplasmic reticulum;Glycoprotein;Hydrolase;Ichthyosis;Leukodystrophy;Lipid metabolism;Lysosome;Metachromatic leukodystrophy;Metal-binding;Reference proteome;Signal SUBCELLULAR LOCATION: Endoplasmic reticulum Lysosome MISCELLANEOUS: The metal cofactor was first identified as magnesium ion, based on the structure of the recombinant protein, but when purified from human placenta, the protein contains 1 calcium ion per subunit." P15314,"PROTEIN NAMES: Interferon regulatory factor 1 (IRF-1) PROTEIN FAMILY: IRF family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the IRF family. FUNCTION: Transcriptional regulator which displays a remarkable functional diversity in the regulation of cellular responses. Regulates transcription of IFN and IFN-inducible genes, host response to viral and bacterial infections, regulation of many genes expressed during hematopoiesis, inflammation, immune responses and cell proliferation and differentiation, regulation of the cell cycle and induction of growth arrest and programmed cell death following DNA damage. Stimulates both innate and acquired immune responses through the activation of specific target genes and can act as a transcriptional activator and repressor regulating target genes by binding to an interferon-stimulated response element (ISRE) in their promoters. Binds to a consensus sequence in gene promoters (By similarity). Its target genes for transcriptional activation activity are: genes involved in anti-viral response, such as IFN-alpha/beta, RIGI, TNFSF10/TRAIL, ZBP1, OAS1/2, PIAS1/GBP, EIF2AK2/PKR and RSAD2/viperin; antibacterial response, such as GBP2, GBP5, IRGB10 and NOS2/INOS; anti-proliferative response, such as p53/TP53, LOX and CDKN1A; apoptosis, such as BBC3/PUMA, CASP1, CASP7 and CASP8; immune response, such as IL7, IL12A/B and IL15, PTGS2/COX2 and CYBB; DNA damage responses and DNA repair, such as POLQ/POLH; MHC class I expression, such as TAP1, PSMB9/LMP2, PSME1/PA28A, PSME2/PA28B and B2M and MHC class II expression, such as CIITA; metabolic enzymes, such as ACOD1/IRG1. Represses genes involved in anti-proliferative response, such as BIRC5/survivin, CCNB1, CCNE1, CDK1, CDK2 and CDK4 and in immune response, such as FOXP3, IL4, ANXA2 and TLR4. Stimulates p53/TP53-dependent transcription through enhanced recruitment of EP300 leading to increased acetylation of p53/TP53 (By similarity). Plays an important role in immune response directly affecting NK maturation and activity, macrophage production of IL12, Th1 development and maturation of CD8+ T-cells. Also implicated in the differentiation and maturation of dendritic cells and in the suppression of regulatory T (Treg) cells development. Acts as a tumor suppressor and plays a role not only in antagonism of tumor cell growth but also in stimulating an immune response against tumor cells. KEYWORDS: 3D-structure;Acetylation;Activator;Antiviral defense;Cytoplasm;DNA-binding;Immunity;Innate immunity;Isopeptide bond;Nucleus;Phosphoprotein;Reference proteome;Repressor;Transcription;Transcription regulation;Ubl conjugation SUBCELLULAR LOCATION: Nucleus Cytoplasm Note=MYD88-associated IRF1 migrates into the nucleus more efficiently than non-MYD88-associated IRF1. " P15330,"PROTEIN NAMES: Embryonic polarity protein dorsal ORGANISM: Drosophila melanogaster (Fruit fly) FUNCTION: Embryonic developmental transcription factor. The lateral or ventral identity of a cell depends upon the concentration of this protein in its nucleus during the blastoderm stage. Acts as a morphogenetic transcription factor that specifically binds to the kappa-B-related consensus sequence 5'-GRGAAAANCC-3', located in the enhancer region of zygotic genes such as Zen, Twist, Snail and Decapentaplegic, promoting their expression. Part of a signaling pathway involving NF-kappa-B and Toll-related receptors, that functions in the apoptosis of unfit cells during cell competition. Mediates an immune response in larvae. May be part of a NF-kappa-B and Tollo signaling cascade that regulates development of the peripheral nervous system. KEYWORDS: Activator;Alternative splicing;Cytoplasm;Developmental protein;DNA-binding;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Cytoplasm Nucleus Note=In ventral regions it is first cytoplasmic, then the protein is relocalized in the nucleus. Its nuclear localization is essential to its function as a morphogen. In dorsal regions it remains cytoplasmic. Tamo negatively regulates nuclear import of dl. Emb is responsible for export of dl from the nucleus. Nuclear localization is enhanced upon microbial infection.; SUBCELLULAR LOCATION: [Isoform A]: Nucleus MISCELLANEOUS: [Isoform A]: Nuclear localization signal at positions 335-340." P15493,"PROTEIN NAMES: Triacylglycerol lipase (Extracellular lipase) (Triacylglycerol ester hydrolase) PROTEIN FAMILY: AB hydrolase superfamily, Pseudomonas lipase family ORGANISM: Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) SIMILARITY: Belongs to the AB hydrolase superfamily. Pseudomonas lipase family. FUNCTION: Catalyzes the hydrolysis of triacylglycerol. KEYWORDS: Calcium;Disulfide bond;Hydrolase;Lipid degradation;Lipid metabolism;Metal-binding;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted MISCELLANEOUS: The lipase chaperone LifO is required for the folding of this protein during its passage through the periplasm." P15509,"PROTEIN NAMES: Granulocyte-macrophage colony-stimulating factor receptor subunit alpha (GM-CSF-R-alpha) (GMCSFR-alpha) (GMR-alpha) (CDw116) (CD antigen CD116) PROTEIN FAMILY: Type I cytokine receptor family, Type 5 subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the type I cytokine receptor family. Type 5 subfamily. FUNCTION: Low affinity receptor for granulocyte-macrophage colony-stimulating factor. Transduces a signal that results in the proliferation, differentiation, and functional activation of hematopoietic cells. KEYWORDS: 3D-structure;Alternative splicing;Cell membrane;Disease variant;Disulfide bond;Glycoprotein;Membrane;Receptor;Reference proteome;Secreted;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein.; SUBCELLULAR LOCATION: [Isoform 3]: Secreted.; SUBCELLULAR LOCATION: [Isoform 4]: Secreted.; SUBCELLULAR LOCATION: [Isoform 6]: Secreted MISCELLANEOUS: The gene coding for this protein is located in the pseudoautosomal region 1 (PAR1) of X and Y chromosomes." P15625,"PROTEIN NAMES: Phenylalanine--tRNA ligase alpha subunit (Phenylalanyl-tRNA synthetase alpha subunit) (PheRS) PROTEIN FAMILY: Class-II aminoacyl-tRNA synthetase family, Phe-tRNA synthetase alpha subunit type 2 subfamily ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha subunit type 2 subfamily. KEYWORDS: Acetylation;Aminoacyl-tRNA synthetase;ATP-binding;Cytoplasm;Direct protein sequencing;Ligase;Magnesium;Metal-binding;Nucleotide-binding;Protein biosynthesis;Reference proteome SUBCELLULAR LOCATION: Cytoplasm. " P15703,"PROTEIN NAMES: Glucan 1,3-beta-glucosidase (Exo-1,3-beta-glucanase) (GP29) (Soluble cell wall protein 9) PROTEIN FAMILY: Glycosyl hydrolase 17 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the glycosyl hydrolase 17 family. FUNCTION: Glucanases possibly play a role in cell expansion during growth, in cell-cell fusion during mating, and in spore release during sporulation. This enzyme may be involved in beta-glucan degradation and also function biosynthetically as a transglycosylase. KEYWORDS: Cell wall;Cell wall biogenesis/degradation;Chitin-binding;Direct protein sequencing;Glycoprotein;Glycosidase;Hydrolase;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted, cell wall Note=Tightly bound to cell wall. MISCELLANEOUS: This protein strongly binds to glucan and chitin.; MISCELLANEOUS: Present with 45000 molecules/cell in log phase SD medium." P15705,PROTEIN NAMES: Heat shock protein STI1 ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: May play a role in mediating the heat shock response of some HSP70 genes. It is required for optimal growth of yeast cells at both low and high temperature. KEYWORDS: 3D-structure;Cytoplasm;Direct protein sequencing;Glycoprotein;Isopeptide bond;Phosphoprotein;Reference proteome;Repeat;Stress response;TPR repeat;Ubl conjugation SUBCELLULAR LOCATION: Cytoplasm MISCELLANEOUS: Present with 67600 molecules/cell in log phase SD medium. P15735,"PROTEIN NAMES: Phosphorylase b kinase gamma catalytic chain, liver/testis isoform (PHK-gamma-LT) (PHK-gamma-T) (PSK-C3) (Phosphorylase kinase subunit gamma-2) PROTEIN FAMILY: Protein kinase superfamily, CAMK Ser/Thr protein kinase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. FUNCTION: Catalytic subunit of the phosphorylase b kinase (PHK), which mediates the neural and hormonal regulation of glycogen breakdown (glycogenolysis) by phosphorylating and thereby activating glycogen phosphorylase. May regulate glycogeneolysis in the testis. In vitro, phosphorylates PYGM (By similarity). KEYWORDS: 3D-structure;Alternative splicing;ATP-binding;Calmodulin-binding;Carbohydrate metabolism;Disease variant;Glycogen metabolism;Glycogen storage disease;Kinase;Nucleotide-binding;Phosphoprotein;Reference proteome;Serine/threonine-protein kinase;Transferase " P15802,"PROTEIN NAMES: Isopenicillin-N N-acyltransferase (IAT) (IPN acyltransferase) (Acyl-coenzyme A:6-aminopenicillanic-acid-acyltransferase 40 kDa form) (Penicillin biosynthetis cluster protein aatA) [Cleaved into: Acyl-coenzyme A:6-aminopenicillanic acid acyltransferase 11 kDa subunit; Acyl-coenzyme A:6-aminopenicillanic acid acyltransferase 29 kDa subunit] PROTEIN FAMILY: Peptidase C45 family ORGANISM: Penicillium chrysogenum (Penicillium notatum) SIMILARITY: Belongs to the peptidase C45 family. FUNCTION: Isopenicillin-N N-acyltransferase; part of the gene cluster that mediates the biosynthesis of penicillin, the world's most important antibiotic. AatA catalyzes the exchange of the alpha-aminoadipyl side chain of isopenicillin N for phenylacetic acid to yield penicillin. This step occurs in the peroxisomal matrix and the penM and paaT transporters are involved in the isopenicillin N and phenylacetic acid import into the peroxisome, respectively. The penicillin biosynthesis occurs via 3 enzymatic steps, the first corresponding to the production of the tripeptide N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine (LLD-ACV or ACV) by the NRPS acvA. The tripeptide ACV is then cyclized to isopenicillin N (IPN) by the isopenicillin N synthase ipnA that forms the beta-lactam nucleus. Finally, the alpha-aminoadipyl side chain is exchanged for phenylacetic acid by the isopenicillin N acyltransferase aatA to yield penicillin in the peroxisomal matrix (Probable). PATHWAY: Antibiotic biosynthesis; penicillin G biosynthesis; penicillin G from L-alpha-aminoadipate and L-cysteine and L-valine: step 3/3. KEYWORDS: 3D-structure;Acyltransferase;Antibiotic biosynthesis;Direct protein sequencing;Peroxisome;Transferase;Zymogen SUBCELLULAR LOCATION: Peroxisome matrix Note=The unprocessed preprotein is translocated inside peroxisomes and regulates its self-processing. " P15863,PROTEIN NAMES: Paired box protein Pax-1 (HuP48) ORGANISM: Homo sapiens (Human) FUNCTION: This protein is a transcriptional activator. It may play a role in the formation of segmented structures of the embryo. May play an important role in the normal development of the vertebral column (By similarity). KEYWORDS: Activator;Alternative splicing;Deafness;Developmental protein;Disease variant;DNA-binding;Intellectual disability;Nucleus;Paired box;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus. P15873,PROTEIN NAMES: Proliferating cell nuclear antigen (PCNA) PROTEIN FAMILY: PCNA family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the PCNA family. FUNCTION: This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Involved in DNA repair. KEYWORDS: 3D-structure;Direct protein sequencing;DNA damage;DNA repair;DNA replication;DNA-binding;Isopeptide bond;Nucleus;Reference proteome;Ubl conjugation SUBCELLULAR LOCATION: Nucleus. P15925,"PROTEIN NAMES: Folylpolyglutamate synthase (FPGS) (Folylpoly-gamma-glutamate synthetase) (Tetrahydrofolylpolyglutamate synthase) PROTEIN FAMILY: Folylpolyglutamate synthase family ORGANISM: Lacticaseibacillus casei (Lactobacillus casei) SIMILARITY: Belongs to the folylpolyglutamate synthase family. FUNCTION: Involved in the conversion of folates to polyglutamate derivatives, and likely functions in the retention of cellular folate pools. Catalyzes successive MgATP-dependent additions of glutamate to a pteroylmonoglutamate substrate, with a high preference for 5,10-methylenetetrahydrofolate (mTHF). Thus, metabolizes mTHF to the tetraglutamate derivative, but longer glutamate chain length products are not observed. Tetrahydrofolate (H4PteGlu) and 10-formyl-H4PteGlu are poorer folate substrates. In contrast to E.coli FolC, this enzyme does not display dihydrofolate synthase activity. KEYWORDS: 3D-structure;ATP-binding;Ligase;Magnesium;Metal-binding;Nucleotide-binding;One-carbon metabolism MISCELLANEOUS: In contrast to many bacteria such as E.coli and Corynebacterium spp., L.casei cannot synthesize folate de novo, and requires exogenous folates for growth. L.casei metabolizes folate to polyglutamates of chain length up to 11, with octa- and nonaglutamates predominating.; MISCELLANEOUS: Kinetic studies are consistent with an ordered Ter-Ter mechanism with MgATP binding first to the enzyme, folate second, and glutamate last. The order of product dissociation from the enzyme is ADP, folate product, and Pi." P15976,"PROTEIN NAMES: Erythroid transcription factor (Eryf1) (GATA-binding factor 1) (GATA-1) (GF-1) (NF-E1 DNA-binding protein) ORGANISM: Homo sapiens (Human) FUNCTION: Transcriptional activator or repressor which serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence 5'-[AT]GATA[AG]-3' within regulatory regions of globin genes and of other genes expressed in erythroid cells. Activates the transcription of genes involved in erythroid differentiation of K562 erythroleukemia cells, including HBB, HBG1/2, ALAS2 and HMBS. KEYWORDS: 3D-structure;Acetylation;Activator;Alternative initiation;Alternative splicing;Direct protein sequencing;Disease variant;DNA-binding;Hereditary hemolytic anemia;Isopeptide bond;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Repeat;Repressor;Transcription;Transcription regulation;Ubl conjugation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: [Isoform 3]: Produced by alternative initiation at Met-84 of isoform 1." P16140,"PROTEIN NAMES: V-type proton ATPase subunit B (V-ATPase subunit B) (V-ATPase 57 kDa subunit) (Vacuolar proton pump subunit B) PROTEIN FAMILY: ATPase alpha/beta chains family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the ATPase alpha/beta chains family. FUNCTION: Non-catalytic subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments. KEYWORDS: 3D-structure;ATP-binding;Direct protein sequencing;Hydrogen ion transport;Ion transport;Isopeptide bond;Membrane;Nucleotide-binding;Phosphoprotein;Reference proteome;Transport;Ubl conjugation;Vacuole SUBCELLULAR LOCATION: Vacuole membrane ; Peripheral membrane protein ; Cytoplasmic side MISCELLANEOUS: Present with 131000 molecules/cell in log phase SD medium." P16228,"PROTEIN NAMES: Cathepsin E PROTEIN FAMILY: Peptidase A1 family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the peptidase A1 family. FUNCTION: May have a role in immune function. Probably involved in the processing of antigenic peptides during MHC class II-mediated antigen presentation. May play a role in activation-induced lymphocyte depletion in the thymus, and in neuronal degeneration and glial cell activation in the brain (By similarity). KEYWORDS: Alternative splicing;Aspartyl protease;Autocatalytic cleavage;Direct protein sequencing;Disulfide bond;Endosome;Glycoprotein;Hydrolase;Protease;Reference proteome;Signal;Zymogen SUBCELLULAR LOCATION: Endosome Note=The proenzyme is localized to the endoplasmic reticulum and Golgi apparatus, while the mature enzyme is localized to the endosome. MISCELLANEOUS: Administration of dexamethasone results in the conversion of the proenzyme to the mature form in thymocytes." P16387,"PROTEIN NAMES: Pyruvate dehydrogenase E1 component subunit alpha, mitochondrial (Pyruvate dehydrogenase complex component E1 alpha) (PDHE1-A) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). KEYWORDS: Direct protein sequencing;Mitochondrion;Oxidoreductase;Phosphoprotein;Pyruvate;Reference proteome;Thiamine pyrophosphate;Transit peptide SUBCELLULAR LOCATION: Mitochondrion matrix. MISCELLANEOUS: Present with 100000 molecules/cell in log phase SD medium." P16442,"PROTEIN NAMES: Histo-blood group ABO system transferase (Fucosylglycoprotein 3-alpha-galactosyltransferase) (Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase) (Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase) (Glycoprotein-fucosylgalactoside alpha-galactosyltransferase) (Histo-blood group A transferase) (A transferase) (Histo-blood group B transferase) (B transferase) (NAGAT) [Cleaved into: Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase soluble form] PROTEIN FAMILY: Glycosyltransferase 6 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the glycosyltransferase 6 family. FUNCTION: This protein is the basis of the ABO blood group system. The histo-blood group ABO involves three carbohydrate antigens: A, B, and H. A, B, and AB individuals express a glycosyltransferase activity that converts the H antigen to the A antigen (by addition of UDP-GalNAc) or to the B antigen (by addition of UDP-Gal), whereas O individuals lack such activity.; FUNCTION: Glycosyltransferase that catalyzes the transfer of carbohydrates to H antigen, forming the antigenic structures of the ABO blood group. PATHWAY: Protein modification; protein glycosylation. KEYWORDS: 3D-structure;Blood group antigen;Direct protein sequencing;Glycoprotein;Glycosyltransferase;Golgi apparatus;Manganese;Membrane;Metal-binding;Reference proteome;Secreted;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein Secreted. Note=Membrane-bound form in trans cisternae of Golgi. Secreted into the body fluid. " P16451,"PROTEIN NAMES: Pyruvate dehydrogenase complex protein X component, mitochondrial (Dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenase complex) (E3-binding protein) (Pyruvate dehydrogenase complex component E3BP) PROTEIN FAMILY: 2-oxoacid dehydrogenase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the 2-oxoacid dehydrogenase family. FUNCTION: Required for anchoring dihydrolipoamide dehydrogenase (E3) to the dihydrolipoamide transacetylase (E2) core of the pyruvate dehydrogenase complexes of eukaryotes. This specific binding is essential for a functional PDH complex. KEYWORDS: Direct protein sequencing;Lipoyl;Mitochondrion;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Mitochondrion matrix. MISCELLANEOUS: Present with 414 molecules/cell in log phase SD medium." P16603,PROTEIN NAMES: NADPH--cytochrome P450 reductase (CPR) (P450R) PROTEIN FAMILY: NADPH--cytochrome P450 reductase family; Flavodoxin family; Flavoprotein pyridine nucleotide cytochrome reductase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the NADPH--cytochrome P450 reductase family.; SIMILARITY: In the N-terminal section; belongs to the flavodoxin family.; SIMILARITY: In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. FUNCTION: This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5. Involved in ergosterol biosynthesis. Has NADPH-dependent ferrireductase activity on the plasma membrane. KEYWORDS: 3D-structure;Cell membrane;Direct protein sequencing;Endoplasmic reticulum;FAD;Flavoprotein;FMN;Isopeptide bond;Lipid biosynthesis;Lipid metabolism;Membrane;Mitochondrion;Mitochondrion outer membrane;NADP;Oxidoreductase;Phosphoprotein;Reference proteome;Steroid biosynthesis;Steroid metabolism;Sterol biosynthesis;Sterol metabolism;Transmembrane;Transmembrane helix;Ubl conjugation SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Single-pass membrane protein ; Cytoplasmic side Mitochondrion outer membrane ; Single-pass membrane protein ; Cytoplasmic side Cell membrane ; Single-pass membrane protein ; Cytoplasmic side MISCELLANEOUS: Present with 46600 molecules/cell in log phase SD medium. P16658,"PROTEIN NAMES: tRNA-splicing endonuclease subunit SEN2 (Splicing endonuclease protein 2) (tRNA-intron endonuclease SEN2) PROTEIN FAMILY: TRNA-intron endonuclease family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the tRNA-intron endonuclease family. FUNCTION: Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. This subunit may anchor the endonuclease complex to the nuclear membrane. Probably carries the active site for 5'-splice site cleavage. KEYWORDS: Coiled coil;Lyase;Membrane;Mitochondrion;Mitochondrion outer membrane;Nucleus;Reference proteome;tRNA processing SUBCELLULAR LOCATION: Nucleus Endomembrane system ; Peripheral membrane protein Mitochondrion outer membrane ; Peripheral membrane protein ; Cytoplasmic side Note=The tRNA splicing endonuclease complex is predominantly associated with the outer membrane of mitochondria, suggesting that tRNA splicing mainly takes place on the mitochondrial surface. MISCELLANEOUS: The tRNA splicing endonuclease complex is present with 100 molecules/cell.; MISCELLANEOUS: Present with 319 molecules/cell in log phase SD medium." P16671,"PROTEIN NAMES: Platelet glycoprotein 4 (Fatty acid translocase) (FAT) (Glycoprotein IIIb) (GPIIIB) (Leukocyte differentiation antigen CD36) (PAS IV) (PAS-4) (Platelet collagen receptor) (Platelet glycoprotein IV) (GPIV) (Thrombospondin receptor) (CD antigen CD36) PROTEIN FAMILY: CD36 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the CD36 family. FUNCTION: Multifunctional glycoprotein that acts as a receptor for a broad range of ligands. Ligands can be of proteinaceous nature like thrombospondin, fibronectin, collagen or amyloid-beta as well as of lipidic nature such as oxidized low-density lipoprotein (oxLDL), anionic phospholipids, long-chain fatty acids and bacterial diacylated lipopeptides. They are generally multivalent and can therefore engage multiple receptors simultaneously, the resulting formation of CD36 clusters initiates signal transduction and internalization of receptor-ligand complexes. The dependency on coreceptor signaling is strongly ligand specific. Cellular responses to these ligands are involved in angiogenesis, inflammatory response, fatty acid metabolism, taste and dietary fat processing in the intestine (Probable). Binds long-chain fatty acids and facilitates their transport into cells, thus participating in muscle lipid utilization, adipose energy storage, and gut fat absorption (By similarity). Mechanistically, binding of fatty acids activates downstream kinase LYN, which phosphorylates the palmitoyltransferase ZDHHC5 and inactivates it, resulting in the subsequent depalmitoylation of CD36 and caveolar endocytosis. In the small intestine, plays a role in proximal absorption of dietary fatty acid and cholesterol for optimal chylomicron formation, possibly through the activation of MAPK1/3 (ERK1/2) signaling pathway (By similarity). Involved in oral fat perception and preferences. Detection into the tongue of long-chain fatty acids leads to a rapid and sustained rise in flux and protein content of pancreatobiliary secretions (By similarity). In taste receptor cells, mediates the induction of an increase in intracellular calcium levels by long-chain fatty acids, leading to the activation of the gustatory neurons in the nucleus of the solitary tract (By similarity). Important factor in both ventromedial hypothalamus neuronal sensing of long-chain fatty acid and the regulation of energy and glucose homeostasis (By similarity). Receptor for thrombospondins, THBS1 and THBS2, mediating their antiangiogenic effects (By similarity). Involved in inducing apoptosis in podocytes in response to elevated free fatty acids, acting together with THBS1 (By similarity). As a coreceptor for TLR4:TLR6 heterodimer, promotes inflammation in monocytes/macrophages. Upon ligand binding, such as oxLDL or amyloid-beta 42, interacts with the heterodimer TLR4:TLR6, the complex is internalized and triggers inflammatory response, leading to NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion, through the priming and activation of the NLRP3 inflammasome (By similarity). Selective and nonredundant sensor of microbial diacylated lipopeptide that signal via TLR2:TLR6 heterodimer, this cluster triggers signaling from the cell surface, leading to the NF-kappa-B-dependent production of TNF, via MYD88 signaling pathway and subsequently is targeted to the Golgi in a lipid-raft dependent pathway (By similarity).; FUNCTION: (Microbial infection) Directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes and the internalization of particles independently of TLR signaling. KEYWORDS: 3D-structure;Alternative splicing;Cell adhesion;Cell membrane;Direct protein sequencing;Disease variant;Disulfide bond;Glycoprotein;Golgi apparatus;Isopeptide bond;Lipid transport;Lipoprotein;Membrane;Palmitate;Receptor;Reference proteome;Transmembrane;Transmembrane helix;Transport;Ubl conjugation SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Membrane raft Golgi apparatus Apical cell membrane Note=Upon ligand-binding, internalized through dynamin-dependent endocytosis. " P16686,"PROTEIN NAMES: Alpha-D-ribose 1-methylphosphonate 5-triphosphate synthase subunit PhnH (RPnTP synthase subunit PhnH) PROTEIN FAMILY: PhnH family ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the PhnH family. FUNCTION: Together with PhnG, PhnI and PhnL is required for the transfer of the ribose triphosphate moiety from ATP to methyl phosphonate. KEYWORDS: 3D-structure;Reference proteome;Transferase MISCELLANEOUS: The sequence shown is that of strains K12 and B." P16692,"PROTEIN NAMES: Phosphoribosyl 1,2-cyclic phosphate phosphodiesterase (Phosphoribosyl cyclic phosphodiesterase) ORGANISM: Escherichia coli (strain K12) FUNCTION: Catalyzes the hydrolysis of the cyclic ribose-phosphate to form alpha-D-ribose 1,5-bisphosphate. KEYWORDS: 3D-structure;Hydrolase;Manganese;Metal-binding;Reference proteome;Zinc MISCELLANEOUS: The sequence shown is that of strains K12 and B." P16869,"PROTEIN NAMES: Hydroxamate siderophore receptor FhuE (Ferric iron uptake protein) (Ferric-coprogen receptor FhuE) (Outer-membrane receptor for Fe(III)-coprogen, Fe(III)-ferrioxamine B and Fe(III)-rhodotrulic acid) (TonB-dependent transporter receptor FhuE) (TBDT receptor FhuE) PROTEIN FAMILY: TonB-dependent receptor family ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the TonB-dependent receptor family. FUNCTION: Involved in the active transport across the outer membrane of iron complexed with linear hydroxamate siderophores coprogen, rhodotorulic acid and ferrioxamine B. Binds Fe-coprogen with high affinity, rhodotorulic acid to a lesser extent, and weakly to ferrioxamine B. Selective for planar siderophores. Does not use cyclic siderophores ferrichrome nor ferrioxamine E as substrates. KEYWORDS: 3D-structure;Cell outer membrane;Direct protein sequencing;Ion transport;Iron;Iron transport;Membrane;Receptor;Reference proteome;Signal;TonB box;Transmembrane;Transmembrane beta strand;Transport SUBCELLULAR LOCATION: Cell outer membrane ; Multi-pass membrane protein " P16912,"PROTEIN NAMES: cAMP-dependent protein kinase catalytic subunit 3 (Protein kinase DC2) PROTEIN FAMILY: Protein kinase superfamily, AGC Ser/Thr protein kinase family, cAMP subfamily ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily. FUNCTION: Does not have an essential role in development. KEYWORDS: Alternative splicing;ATP-binding;Kinase;Nucleotide-binding;Reference proteome;Serine/threonine-protein kinase;Transferase " P17010,"PROTEIN NAMES: Zinc finger X-chromosomal protein PROTEIN FAMILY: Krueppel C2H2-type zinc-finger protein family, ZFX/ZFY subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein family. ZFX/ZFY subfamily. FUNCTION: Probable transcriptional activator. KEYWORDS: Activator;Alternative splicing;DNA-binding;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Repeat;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus. " P17064,"PROTEIN NAMES: Purine-cytosine permease FCY2 (PCP FCY2) (Cytosine/purine transport protein FCY2) (Fluorocytosine resistance protein 2) PROTEIN FAMILY: Purine-cytosine permease (2.A.39) family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the purine-cytosine permease (2.A.39) family. FUNCTION: This permease has a broad specificity towards purines, and also transport cytosine and 5-methylcytosine but neither uracil nor thymine. KEYWORDS: Isopeptide bond;Membrane;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix;Transport;Ubl conjugation SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. MISCELLANEOUS: Present with 23600 molecules/cell in log phase SD medium." P17065,"PROTEIN NAMES: Rab guanine nucleotide exchange factor SEC2 (GDP-GTP exchange factor SEC2) PROTEIN FAMILY: SEC2 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the SEC2 family. FUNCTION: Guanine nucleotide exchange factor for SEC4, catalyzing the dissociation of GDP from SEC4 and also potently promoting binding of GTP. Activation of SEC4 by SEC2 is needed for the directed transport of vesicles to sites of exocytosis. Binds the Rab GTPase YPT32, but does not have exchange activity on YPT32. KEYWORDS: 3D-structure;Acetylation;Coiled coil;Cytoplasmic vesicle;Guanine-nucleotide releasing factor;Phosphoprotein;Protein transport;Reference proteome;Transport SUBCELLULAR LOCATION: Bud neck Bud tip Cytoplasmic vesicle, secretory vesicle Note=Localizes to sites of polarized growth, namely to the bud neck and to the bud tip of growing buds, and associates with membranes. Colocalizes with secretory vesicles at exocytic sites. Proper localization is dependent on the actin cytoskeleton, MYO2, the kinesin-related protein SMY1, the Rab GTPase YPT32, and the production of post-Golgi vesicles. MISCELLANEOUS: Present with 13200 molecules/cell in log phase SD medium." P17119,"PROTEIN NAMES: Kinesin-like protein KAR3 (Nuclear fusion protein) PROTEIN FAMILY: TRAFAC class myosin-kinesin ATPase superfamily, Kinesin family, NCD subfamily ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. NCD subfamily. FUNCTION: Minus end-directed microtubule (MT) motor involved in spindle midzone assembly, poleward transport of newly captured kinetochores along the lateral side of MTs, karyogamy (nuclear fusion) during mating, and with an essential function in meiosis I. Functions together with the accessory proteins CIK1 or VIK1. Drives the poleward transport of newly captured kinetochores along the lateral side of MTs, both during S-phase and during M-phase. To contribute to spindle midzone assembly during mitotic metaphase, the nuclear KAR3-CIK1 motor cross-links anti-parallel microtubules to align them on the spindle axis; as the motor travels polewards splayed microtubules are pulled into alignment. During the karyogamy (nuclear fusion) step of mating, KAR3-CIK1 cross-links antiparallel cytoplasmic microtubules emanating from the spindle pole bodies of mating partners; the motor activity of KAR3 creates the force that pulls the nuclei together by sliding cross-linked microtubules past one another. KAR3-CIK1 promotes microtubule shortening predominantly from the microtubule plus-end. Together with cytoplasmic VIK1, may act to stabilize microtubules. Requires accessory protein VIK1 for spindle pole body localization and to allow the CIN8 and KIP1 motors to generate outwardly directed spindle forces. Essential during meiosis I. The ATPase activity is stimulated by microtubule-binding. KEYWORDS: 3D-structure;ATP-binding;Cell cycle;Cell division;Chromosome;Coiled coil;Cytoplasm;Cytoskeleton;Isomerase;Karyogamy;Microtubule;Motor protein;Nucleotide-binding;Nucleus;Reference proteome SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body Nucleus Cytoplasm, cytoskeleton Chromosome Cytoplasm, cytoskeleton, spindle Note=Cytoplasmic microtubules. MISCELLANEOUS: KAR3 contains two globular domains separated by an alpha-helical coiled coil. The N-terminal portion of KAR3 contains a microtubule association domain distinct from the kinesin-like C-terminal domain.; MISCELLANEOUS: Present with 3250 molecules/cell in log phase SD medium." P17133,"PROTEIN NAMES: U1 small nuclear ribonucleoprotein 70 kDa (U1 snRNP 70 kDa) (U1-70K) (snRNP70) ORGANISM: Drosophila melanogaster (Fruit fly) FUNCTION: Mediates the splicing of pre-mRNA by binding to the stem loop I region of U1-snRNA. Required during oogenesis for nurse cell chromatin dispersal. KEYWORDS: 3D-structure;mRNA processing;Nucleus;Reference proteome;Ribonucleoprotein;RNA-binding SUBCELLULAR LOCATION: Nucleus speckle Nucleus, nucleoplasm " P17177,"PROTEIN NAMES: Sterol 26-hydroxylase, mitochondrial (5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol 26-hydroxylase) (Cytochrome P-450C27/25) (Cytochrome P450 27) (Sterol 27-hydroxylase) (Vitamin D(3) 25-hydroxylase) PROTEIN FAMILY: Cytochrome P450 family ORGANISM: Oryctolagus cuniculus (Rabbit) SIMILARITY: Belongs to the cytochrome P450 family. FUNCTION: Cytochrome P450 monooxygenase that catalyzes regio- and stereospecific hydroxylation of cholesterol and its derivatives. Hydroxylates (with R stereochemistry) the terminal methyl group of cholesterol side-chain in a three step reaction to yield at first a C26 alcohol, then a C26 aldehyde and finally a C26 acid (By similarity). Regulates cholesterol homeostasis by catalyzing the conversion of excess cholesterol to bile acids via both the 'neutral' (classic) and the 'acid' (alternative) pathways. May also regulate cholesterol homeostasis via generation of active oxysterols, which act as ligands for NR1H2 and NR1H3 nuclear receptors, modulating the transcription of genes involved in lipid metabolism. Plays a role in cholestanol metabolism in the cerebellum. Similarly to cholesterol, hydroxylates cholestanol and may facilitate sterol diffusion through the blood-brain barrier to the systemic circulation for further degradation. Also hydroxylates retinal 7-ketocholesterol, a noxious oxysterol with pro-inflammatory and pro-apoptotic effects, and may play a role in its elimination from the retinal pigment epithelium. May play a redundant role in vitamin D biosynthesis. Catalyzes 25-hydroxylation of vitamin D3 that is required for its conversion to a functionally active form (By similarity). PATHWAY: Hormone biosynthesis; cholecalciferol biosynthesis.; PATHWAY: Steroid metabolism; cholesterol degradation.; PATHWAY: Lipid metabolism; bile acid biosynthesis. KEYWORDS: Acetylation;Cholesterol metabolism;Direct protein sequencing;Heme;Iron;Lipid biosynthesis;Lipid metabolism;Membrane;Metal-binding;Mitochondrion;Mitochondrion inner membrane;Monooxygenase;Oxidoreductase;Reference proteome;Steroid biosynthesis;Steroid metabolism;Sterol metabolism;Transit peptide SUBCELLULAR LOCATION: Mitochondrion inner membrane ; Peripheral membrane protein Note=Post-translationally targeted to mitochondria. All three of the receptor proteins in the TOM complex, TOMM70, TOMM20 and TOMM22 are required for the translocation across the mitochondrial outer membrane. After translocation into the matrix, associates with the inner membrane as a membrane extrinsic protein. " P17213,"PROTEIN NAMES: Bactericidal permeability-increasing protein (BPI) (CAP 57) PROTEIN FAMILY: BPI/LBP/Plunc superfamily, BPI/LBP family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the BPI/LBP/Plunc superfamily. BPI/LBP family. FUNCTION: The cytotoxic action of BPI is limited to many species of Gram-negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope. Has antibacterial activity against the Gram-negative bacterium P.aeruginosa, this activity is inhibited by LPS from P.aeruginosa. KEYWORDS: 3D-structure;Antibiotic;Antimicrobial;Direct protein sequencing;Disulfide bond;Glycoprotein;Immunity;Innate immunity;Membrane;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted Cytoplasmic granule membrane Note=Membrane-associated in polymorphonuclear Leukocytes (PMN) granules. " P17221,"PROTEIN NAMES: Sex-determining protein fem-1 (Feminization of XX and XO animals protein 1) PROTEIN FAMILY: Fem-1 family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the fem-1 family. FUNCTION: Substrate-recognition component of the cullin-RING-based CBC(fem-1) (Cul2-ElonginB-ElonginC) E3 ubiquitin-protein ligase complex of the DesCEND (destruction via C-end degrons) pathway, which recognizes a C-degron located at the extreme C terminus of target proteins, leading to their ubiquitination and degradation (By similarity). The C-degron recognized by the DesCEND pathway is usually a motif of less than ten residues and can be present in full-length proteins, truncated proteins or proteolytically cleaved forms (By similarity). Sex-determining protein; essential for the adoption of the male sexual fate in all tissues. The CBC(fem-1) complex mediates the ubiquitination and subsequent proteasomal degradation of tra-1. Promotes ubiquitination and degradation of cdl-1. May be involved in turnover of neuronal substrates, thereby impacting synaptic acetylcholine release and neuromuscular transmission. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Alternative splicing;ANK repeat;Cytoplasm;Developmental protein;Differentiation;Phosphoprotein;Reference proteome;Repeat;Sexual differentiation;TPR repeat;Ubl conjugation pathway SUBCELLULAR LOCATION: Cytoplasm " P17260,"PROTEIN NAMES: Protein KRE1 (Killer toxin-resistance protein 1) PROTEIN FAMILY: KRE1 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the KRE1 family. FUNCTION: Involved in a late stage of cell wall 1,6-beta-glucan synthesis and assembly. Has a structural, rather than enzymic, function within cell wall 1,6-beta-glucan assembly and architecture, possibly by being involved in covalently cross-linking 1,6-beta-glucans to other cell wall components such as 1,3-beta-glucan, chitin and certain mannoproteins. Acts as the plasma membrane receptor for the yeast K1 viral toxin. KEYWORDS: Cell membrane;Cell wall;Cell wall biogenesis/degradation;Glycoprotein;GPI-anchor;Lipoprotein;Membrane;Reference proteome;Repeat;Secreted;Signal SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor, GPI-anchor. Secreted, cell wall. Note=Identified as GPI-anchored plasma membrane protein (GPI-PMP) as well as component of the cell wall. Concentrated at the surface of mother cells. MISCELLANEOUS: Present with 623 molecules/cell in log phase SD medium." P17454,"PROTEIN NAMES: Lipopolysaccharide-binding protein (LBP) PROTEIN FAMILY: BPI/LBP/Plunc superfamily, BPI/LBP family ORGANISM: Oryctolagus cuniculus (Rabbit) SIMILARITY: Belongs to the BPI/LBP/Plunc superfamily. BPI/LBP family. FUNCTION: Plays a role in the innate immune response. Binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria. Acts as an affinity enhancer for CD14, facilitating its association with LPS (By similarity). Promotes the release of cytokines in response to bacterial lipopolysaccharide. KEYWORDS: Antibiotic;Antimicrobial;Direct protein sequencing;Disulfide bond;Glycoprotein;Immunity;Innate immunity;Lipid transport;Reference proteome;Secreted;Signal;Transport SUBCELLULAR LOCATION: Secreted " P17555,"PROTEIN NAMES: Adenylyl cyclase-associated protein (CAP) PROTEIN FAMILY: CAP family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the CAP family. FUNCTION: The N-terminal domain binds to adenylyl cyclase, thereby enabling adenylyl cyclase to be activated by upstream regulatory signals, such as Ras. The C-terminal domain is required for normal cellular morphology and growth control. KEYWORDS: 3D-structure;Actin-binding;Cytoplasm;Cytoskeleton;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, actin patch Note=Cortical actin patches. MISCELLANEOUS: Present with 8760 molecules/cell in log phase SD medium." P17597,"PROTEIN NAMES: Acetolactate synthase, chloroplastic (AtALS) (Acetohydroxy-acid synthase) (Protein CHLORSULFURON RESISTANT 1) PROTEIN FAMILY: TPP enzyme family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the TPP enzyme family. FUNCTION: Catalyzes the formation of acetolactate from pyruvate, the first step in valine and isoleucine biosynthesis. PATHWAY: Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 1/4.; PATHWAY: Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 1/4. KEYWORDS: 3D-structure;Amino-acid biosynthesis;Branched-chain amino acid biosynthesis;Chloroplast;Coiled coil;FAD;Flavoprotein;Genetically modified food;Herbicide resistance;Magnesium;Metal-binding;Oxidation;Plastid;Reference proteome;Thiamine pyrophosphate;Transferase;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast " P17709,"PROTEIN NAMES: Glucokinase-1 (Glucose kinase 1) (GLK-1) PROTEIN FAMILY: Hexokinase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the hexokinase family. FUNCTION: Two isoenzymes, hexokinase-1 and hexokinase-2, can phosphorylate keto- and aldohexoses in yeast, whereas a third isoenzyme, GLK, is specific for aldohexoses. All glucose phosphorylating enzymes are involved in glucose uptake. PATHWAY: Carbohydrate metabolism; hexose metabolism.; PATHWAY: Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 1/4. KEYWORDS: 3D-structure;Acetylation;ATP-binding;Glycolysis;Kinase;Nucleotide-binding;Phosphoprotein;Reference proteome;Transferase MISCELLANEOUS: Present with 21100 molecules/cell in log phase SD medium." P17813,"PROTEIN NAMES: Endoglin (CD antigen CD105) ORGANISM: Homo sapiens (Human) FUNCTION: Vascular endothelium glycoprotein that plays an important role in the regulation of angiogenesis. Required for normal structure and integrity of adult vasculature. Regulates the migration of vascular endothelial cells. Required for normal extraembryonic angiogenesis and for embryonic heart development (By similarity). May regulate endothelial cell shape changes in response to blood flow, which drive vascular remodeling and establishment of normal vascular morphology during angiogenesis (By similarity). May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors. Acts as a TGF-beta coreceptor and is involved in the TGF-beta/BMP signaling cascade that ultimately leads to the activation of SMAD transcription factors. Required for GDF2/BMP9 signaling through SMAD1 in endothelial cells and modulates TGFB1 signaling through SMAD3. KEYWORDS: 3D-structure;Alternative splicing;Angiogenesis;Cell adhesion;Cell membrane;Direct protein sequencing;Disease variant;Disulfide bond;Glycoprotein;Membrane;Phosphoprotein;Reference proteome;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein " P17892,"PROTEIN NAMES: Pancreatic lipase-related protein 2 (PL-RP2) (Cytotoxic T lymphocyte lipase) (Galactolipase) (Triacylglycerol lipase) PROTEIN FAMILY: AB hydrolase superfamily, Lipase family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the AB hydrolase superfamily. Lipase family. FUNCTION: Lipase that primarily hydrolyzes triglycerides and galactosylglycerides. In neonates, may play a major role in pancreatic digestion of dietary fats such as milk fat globules enriched in long-chain triglycerides. Hydrolyzes short-, medium- and long-chain fatty acyls in triglycerides without apparent positional specificity. Can completely deacylate triacylglycerols (By similarity). When the liver matures and bile salt synthesis increases, likely functions mainly as a galactolipase and monoacylglycerol lipase. Hydrolyzes monogalactosyldiglycerols (MGDG) and digalactosyldiacylglycerols (DGDG) present in a plant-based diet, releasing long-chain polyunsaturated fatty acids (By similarity). Hydrolyzes medium- and long-chain fatty acyls in galactolipids (By similarity). May act together with LIPF to hydrolyze partially digested triglycerides (By similarity). Hydrolyzes long-chain monoglycerides with high efficiency (By similarity). In cytotoxic T cells, contributes to perforin-dependent cell lysis, but is unlikely to mediate direct cytotoxicity. Also has low phospholipase activity (By similarity). In neurons, required for the localization of the phospholipid 1-oleoyl-2-palmitoyl-PC (OPPC) to neurite tips through acyl chain remodeling of membrane phospholipids (By similarity). The resulting OPPC-rich lipid membrane domain recruits the t-SNARE protein STX4 by selectively interacting with the STX4 transmembrane domain and this promotes surface expression of the dopamine transporter SLC6A3/DAT at neurite tips by facilitating fusion of SLC6A3-containing transport vesicles with the plasma membrane (By similarity). PATHWAY: Glycerolipid metabolism; triacylglycerol degradation.; PATHWAY: Glycolipid metabolism. KEYWORDS: Calcium;Cell projection;Cytoplasmic vesicle;Disulfide bond;Glycoprotein;Hydrolase;Lipid degradation;Lipid metabolism;Membrane;Metal-binding;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted Zymogen granule membrane ; Peripheral membrane protein Cell projection, neuron projection Note=Localizes to neurite tips in neuronal cells. " P17896,PROTEIN NAMES: SpoIVB peptidase (Sporulation factor IV B protease) (Stage IV sporulation protein B) [Cleaved into: SpoIVB peptidase 45 kDa isoform; SpoIVB peptidase 43 kDa isoform; SpoIVB peptidase 42 kDa isoform] ORGANISM: Bacillus subtilis (strain 168) FUNCTION: Plays a central role in the sigma-K checkpoint which coordinates gene expression during the later stages of spore formation. The protease is activated by trans cleavage of the zymogen precursor producing SpoIVB-45 kDa. This undergoes further trimming by cis cleavage to form SpoIVB-43 kDa and SpoIVB-42 kDa. The protease then cleaves the C-terminus of the SpoIVFA metalloprotease activating the latter. KEYWORDS: Direct protein sequencing;Hydrolase;Protease;Reference proteome;Serine protease;Signal;Sporulation;Zymogen SUBCELLULAR LOCATION: Forespore intermembrane space. P17898,"PROTEIN NAMES: Cholinephosphotransferase 1 (Aminoalcohol phosphotransferase CPT1) (Diacylglycerol cholinephosphotransferase 1) (Sn-1,2-diacylglycerol cholinephosphotransferase) (CHOPT) PROTEIN FAMILY: CDP-alcohol phosphatidyltransferase class-I family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the CDP-alcohol phosphatidyltransferase class-I family. FUNCTION: Catalyzes the final step in the CDP-choline route leading to phosphatidylcholin (PC). Preferentially uses CDP-monomethylethanolamine as aminoalcohol substrate. Shows highest activity toward di- and mono-unsaturated diacylglycerol species as lipid substrates. The CDP-choline pathway only contributes to net PC synthesis if exogenous choline is present. In its absence, this pathway recycles choline from PC turnover and may contribute to maintaining the proper PC species composition. PATHWAY: Phospholipid metabolism; phosphatidylcholine biosynthesis; phosphatidylcholine from phosphocholine: step 2/2. KEYWORDS: Endoplasmic reticulum;Lipid biosynthesis;Lipid metabolism;Magnesium;Membrane;Metal-binding;Microsome;Mitochondrion;Mitochondrion outer membrane;Phospholipid biosynthesis;Phospholipid metabolism;Reference proteome;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Microsome membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Mitochondrion outer membrane; Multi-pass membrane protein. MISCELLANEOUS: Present with 981 molecules/cell in log phase SD medium." P17967,"PROTEIN NAMES: Protein disulfide-isomerase (PDI) (Thioredoxin-related glycoprotein 1) PROTEIN FAMILY: Protein disulfide isomerase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the protein disulfide isomerase family. FUNCTION: Protein disulfide isomerase of ER lumen required for formation of disulfide bonds in secretory and cell-surface proteins and which unscrambles non-native disulfide bonds. Forms a complex with MNL1 to process unfolded protein-bound Man8GlcNAc2 oligosaccharides to Man7GlcNAc2, promoting degradation in unfolded protein response. KEYWORDS: 3D-structure;Direct protein sequencing;Disulfide bond;Endoplasmic reticulum;Glycoprotein;Isomerase;Redox-active center;Reference proteome;Repeat;Signal SUBCELLULAR LOCATION: Endoplasmic reticulum lumen " P17978,"PROTEIN NAMES: Virginiamycin B lyase (Streptogramin B lyase) PROTEIN FAMILY: Vgb family ORGANISM: Staphylococcus aureus SIMILARITY: Belongs to the Vgb family. FUNCTION: Inactivates the type B streptogramin antibiotics by linearizing the lactone ring at the ester linkage, generating a free phenylglycine carboxylate and converting the threonyl moiety into 2-amino-butenoic acid. KEYWORDS: 3D-structure;Antibiotic resistance;Lyase;Magnesium;Metal-binding;Plasmid MISCELLANEOUS: Has unequivocally been shown to cleave the ester bond of streptogramin B antibiotics by an elimination reaction and not by hydrolysis, therefore is a lyase and not a hydrolase. The olefinic dehydrobutyrine thus generated in the N-terminal position has a Z stereochemistry." P18011,"PROTEIN NAMES: Type 3 secretion system translocon protein SctE (T3SS translocon protein SctE) (62 kDa antigen) (Invasin IpaB) PROTEIN FAMILY: SctE/SipB/YopB family ORGANISM: Shigella flexneri SIMILARITY: Belongs to the SctE/SipB/YopB family. FUNCTION: Component of the type III secretion system (T3SS), also called injectisome, which is used to inject bacterial effector proteins into eukaryotic host cells. IpaB/SctE and IpaC/SctB are inserted into the host membrane where they form a pore and allow the translocation of effector proteins into the cytosol of target cells. Interaction with IpaD/SctA at needle tips leads to the formation of the MxiH/SctF-IpaD/SctA-IpaB/SctE ternary complex, which is essential for host cell sensing. Interaction of IpaB/SctE with host membrane lipids promotes recruitment of IpaC/SctB at the needle tip concomitant with translocon insertion into the host membrane and type III secretion induction.; FUNCTION: Required for efficient dissemination. Necessary for lysis of the two cellular membranes that surround bacteria in protrusions during cell-to-cell spread. Is sufficient to induce macrophage apoptosis through activation of the interleukin-1 beta converting enzyme (ICE) in infected macrophages. In epithelial cells, causes cell-cycle arrest by targeting host MAD2L2, an anaphase-promoting complex/cyclosome (APC) inhibitor. KEYWORDS: 3D-structure;Coiled coil;Direct protein sequencing;Host membrane;Host nucleus;Membrane;Plasmid;Reference proteome;Secreted;Transmembrane;Transmembrane helix;Virulence SUBCELLULAR LOCATION: Secreted Host membrane ; Multi-pass membrane protein Host cell Host nucleus Note=Secreted via the type III secretion system (T3SS). Localizes at the surface of needle tips via IpaD/SctA. IpaB/SctE and IpaC/SctB form a multimeric integral membrane complex in eukaryotic cell membranes. Also secreted into the cytoplasm of the infected macrophage after the escape of bacteria from phagosome, where it colocalizes with ICE. May localize to host cell nucleus during G2/M phase of the host cell cycle. " P18012,"PROTEIN NAMES: Type 3 secretion system translocon protein SctB (T3SS translocon protein SctB) (42 kDa antigen) (Invasin IpaC) (Invasion plasmid antigen C) PROTEIN FAMILY: SctB/SipC family ORGANISM: Shigella flexneri SIMILARITY: Belongs to the SctB/SipC family. FUNCTION: Component of the type III secretion system (T3SS), also called injectisome, which is used to inject bacterial effector proteins into eukaryotic host cells. IpaB/SctE and IpaC/SctB are inserted into the host membrane where they form a pore and allow the translocation of effector proteins into the cytosol of target cells. Induction and secretion of IpaC/SctB comprise the final step in triggering the induction of full type III secretion.; FUNCTION: Required for efficient dissemination. Necessary for lysis of the two cellular membranes that surround bacteria in protrusions during cell-to-cell spread. Contribute to actin nucleation in vitro, which may be a necessary step in Shigella invasion. KEYWORDS: Direct protein sequencing;Host membrane;Membrane;Plasmid;Reference proteome;Secreted;Transmembrane;Transmembrane helix;Virulence SUBCELLULAR LOCATION: Secreted Host membrane ; Single-pass membrane protein Note=Secreted via the type III secretion system (T3SS). Localizes at needle tips. IpaB/SctE and IpaC/SctB form a multimeric integral membrane complex in eukaryotic cell membranes. " P18031,"PROTEIN NAMES: Tyrosine-protein phosphatase non-receptor type 1 (Protein-tyrosine phosphatase 1B) (PTP-1B) PROTEIN FAMILY: Protein-tyrosine phosphatase family, Non-receptor class 1 subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily. FUNCTION: Tyrosine-protein phosphatase which acts as a regulator of endoplasmic reticulum unfolded protein response. Mediates dephosphorylation of EIF2AK3/PERK; inactivating the protein kinase activity of EIF2AK3/PERK. May play an important role in CKII- and p60c-src-induced signal transduction cascades. May regulate the EFNA5-EPHA3 signaling pathway which modulates cell reorganization and cell-cell repulsion. May also regulate the hepatocyte growth factor receptor signaling pathway through dephosphorylation of MET. KEYWORDS: 3D-structure;Acetylation;Direct protein sequencing;Endoplasmic reticulum;Hydrolase;Membrane;Oxidation;Phosphoprotein;Protein phosphatase;Reference proteome;S-nitrosylation SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Peripheral membrane protein ; Cytoplasmic side Note=Interacts with EPHA3 at the cell membrane. " P18105,"PROTEIN NAMES: Kinesin-like protein Nod PROTEIN FAMILY: TRAFAC class myosin-kinesin ATPase superfamily, Kinesin family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. FUNCTION: Required for the distributive chromosome segregation of non-exchange chromosomes during meiosis. May be a microtubule motor required to hold distributively 'paired' chromosomes at the metaphase plate until anaphase. KEYWORDS: 3D-structure;ATP-binding;Coiled coil;Cytoplasm;Cytoskeleton;Microtubule;Motor protein;Nucleotide-binding;Reference proteome SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton MISCELLANEOUS: The nod(DTW) mutation is a cold-sensitive recessive lethal mutation." P18123,"PROTEIN NAMES: Catalase isozyme 3 PROTEIN FAMILY: Catalase family ORGANISM: Zea mays (Maize) SIMILARITY: Belongs to the catalase family. FUNCTION: Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Its levels are highest in the light period and are lowest in the dark period, hence it may be important for scavenging hydrogen peroxide at night, rather than during the day. KEYWORDS: Heme;Hydrogen peroxide;Iron;Metal-binding;Mitochondrion;Oxidoreductase;Peroxidase;Reference proteome SUBCELLULAR LOCATION: Mitochondrion. " P18163,"PROTEIN NAMES: Long-chain-fatty-acid--CoA ligase 1 (Arachidonate--CoA ligase) (Long-chain acyl-CoA synthetase 1) (LACS 1) (Long-chain-fatty-acid--CoA ligase, liver isozyme) (Phytanate--CoA ligase) PROTEIN FAMILY: ATP-dependent AMP-binding enzyme family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the ATP-dependent AMP-binding enzyme family. FUNCTION: Catalyzes the conversion of long-chain fatty acids to their active form acyl-CoAs for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses palmitoleate, oleate and linoleate (By similarity). Preferentially activates arachidonate than epoxyeicosatrienoic acids (EETs) or hydroxyeicosatrienoic acids (HETEs). KEYWORDS: Acetylation;ATP-binding;Direct protein sequencing;Endoplasmic reticulum;Fatty acid metabolism;Glycoprotein;Ligase;Lipid metabolism;Magnesium;Membrane;Microsome;Mitochondrion;Mitochondrion outer membrane;Nitration;Nucleotide-binding;Peroxisome;Phosphoprotein;Reference proteome;Signal-anchor;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Mitochondrion outer membrane ; Single-pass type III membrane protein Peroxisome membrane ; Single-pass type III membrane protein Microsome membrane ; Single-pass type III membrane protein Endoplasmic reticulum membrane ; Single-pass type III membrane protein MISCELLANEOUS: 5 rat isozymes encoded by different genes have been described. ACSL6 corresponds to isozyme 2 (ACS2)." P18357,"PROTEIN NAMES: Regulatory protein BlaR1 PROTEIN FAMILY: Peptidase M56 family ORGANISM: Staphylococcus aureus SIMILARITY: Belongs to the peptidase M56 family. FUNCTION: Integral membrane protein involved in sensing of the presence of beta-lactam antibiotics and transduction of the information to the cytoplasm. Mechanistically, activation of the signal transducer involves acylation of a serine in the C-terminal sensor domain upon binding of the beta-lactam antibiotic. In turn, a conformational change occurs and the signal is transmitted from the cell surface to the cytoplasm. There, the zinc protease domain is activated and initiates autoproteolysis as well as cleavage of the transcriptional repressor BlaI leading to derepression of antibiotic resistance genes. KEYWORDS: 3D-structure;Cell membrane;Membrane;Transmembrane;Transmembrane helix;Transposable element SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein " P18412,PROTEIN NAMES: Ty transcription activator TEC1 PROTEIN FAMILY: TEC1 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the TEC1 family. FUNCTION: TEC1 is involved in the activation of TY1 and TY1-mediated gene expression. It is not involved in mating or sporulation processes. KEYWORDS: Acetylation;Activator;DNA-binding;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus. MISCELLANEOUS: Present with 530 molecules/cell in log phase SD medium. P18431,"PROTEIN NAMES: Protein kinase shaggy (Protein zeste-white 3) PROTEIN FAMILY: Protein kinase superfamily, CMGC Ser/Thr protein kinase family, GSK-3 subfamily ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily. FUNCTION: Required for several developmental events such as syncytial blastoderm formation and embryonic segmentation. Is involved in transcriptional regulation. Required for arm phosphorylation. Wg signaling operates by inactivating the sgg repression of en autoactivation. Negatively controls the neuromuscular junction (NMJ) growth in presynaptic motoneurons. Plays a role in the regulation of microtubule dynamics and actin cytoskeleton during embryogenesis. Required for phosphorylation of sra in activated eggs. Essential for completion of meiosis, possibly by triggering calcineurin activation via sra phosphorylation. Phosphorylates microtubule-associated protein futsch in axons. KEYWORDS: Alternative splicing;ATP-binding;Cell projection;Cytoplasm;Cytoskeleton;Developmental protein;Kinase;Meiosis;Nucleotide-binding;Nucleus;Phosphoprotein;Reference proteome;Segmentation polarity protein;Serine/threonine-protein kinase;Synapse;Transferase SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cell cortex. Synapse. Cell projection, axon. Note=In syncytial embryos, detected at the centrosomes throughout the cell cycle, and in the mitotic spindle and pseudocleavage furrows invaginating from the cell cortex during mitosis. Concentrated at the growing end of membranes during the cellularization process. After cellularization, localized to the centrosomes during mitosis and to the nucleus at the end of telophase. Enriched in the presynaptic side of the neuromuscular junction, with some signal detected also in axonal branches. MISCELLANEOUS: [Isoform Zygotic]: Major isoform." P18432,PROTEIN NAMES: Myosin regulatory light chain 2 (MLC-2) ORGANISM: Drosophila melanogaster (Fruit fly) KEYWORDS: Acetylation;Calcium;Metal-binding;Motor protein;Muscle protein;Myosin;Phosphoprotein;Reference proteome;Repeat MISCELLANEOUS: This chain binds calcium.; MISCELLANEOUS: MLC2 has at least two isoforms in each type of muscle and the isoforms of tubular muscle differ slightly from those of fibrillar muscle. These isoforms may arise through post-translational modifications. P18459,"PROTEIN NAMES: Tyrosine 3-monooxygenase (Protein Pale) (Tyrosine 3-hydroxylase) (TH) PROTEIN FAMILY: Biopterin-dependent aromatic amino acid hydroxylase family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the biopterin-dependent aromatic amino acid hydroxylase family. FUNCTION: Plays an important role in the physiology of adrenergic neurons. PATHWAY: Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2. KEYWORDS: Alternative splicing;Catecholamine biosynthesis;Cell projection;Cytoplasm;Iron;Metal-binding;Monooxygenase;Neurotransmitter biosynthesis;Oxidoreductase;Reference proteome SUBCELLULAR LOCATION: Cytoplasm, perinuclear region Cell projection, axon Note=Expressed in dopaminergic axons and axon terminals. " P18488,PROTEIN NAMES: Homeotic protein empty spiracles PROTEIN FAMILY: EMX homeobox family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the EMX homeobox family. FUNCTION: Acts as a homeotic selector gene controlling antennal and mandibular segment identity. KEYWORDS: Developmental protein;DNA-binding;Homeobox;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus. MISCELLANEOUS: The sequence shown is that of strain canton S. P18572,"PROTEIN NAMES: Basigin (Basic immunoglobulin superfamily) (HT7 antigen) (Membrane glycoprotein gp42) (CD antigen CD147) ORGANISM: Mus musculus (Mouse) FUNCTION: [Isoform 1]: Essential for normal retinal maturation and development. Acts as a retinal cell surface receptor for NXNL1 and plays an important role in NXNL1-mediated survival of retinal cone photoreceptors. In association with glucose transporter SLC16A1/GLUT1 and NXNL1, promotes retinal cone survival by enhancing aerobic glycolysis and accelerating the entry of glucose into photoreceptors.; FUNCTION: [Isoform 2]: Signaling receptor for cyclophilins, essential for PPIA/CYPA and PPIB/CYPB-dependent signaling related to chemotaxis and adhesion of immune cells (By similarity). Plays an important role in targeting the monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to the plasma membrane. Acts as a coreceptor for vascular endothelial growth factor receptor 2 (KDR/VEGFR2) in endothelial cells enhancing its VEGFA-mediated activation and downstream signaling (By similarity). Promotes angiogenesis through EPAS1/HIF2A-mediated up-regulation of VEGFA and KDR/VEGFR2 in endothelial cells (By similarity). Plays an important role in spermatogenesis; mediates interactions between germ cells and Sertoli cell and is essential for the development/differentiation of germ cells to round spermatids. KEYWORDS: 3D-structure;Alternative splicing;Angiogenesis;Cell membrane;Cell projection;Differentiation;Direct protein sequencing;Disulfide bond;Endoplasmic reticulum;Glycoprotein;Immunoglobulin domain;Lectin;Mannose-binding;Membrane;Phosphoprotein;Receptor;Reference proteome;Signal;Spermatogenesis;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: [Isoform 1]: Cell membrane ; Single-pass type I membrane protein Photoreceptor inner segment Cell projection, cilium, photoreceptor outer segment.; SUBCELLULAR LOCATION: [Isoform 2]: Cell membrane ; Single-pass type I membrane protein Endoplasmic reticulum membrane ; Single-pass type I membrane protein Basolateral cell membrane ; Single-pass type I membrane protein " P18627,"PROTEIN NAMES: Lymphocyte activation gene 3 protein (LAG-3) (CD antigen CD223) [Cleaved into: Secreted lymphocyte activation gene 3 protein (sLAG-3)] PROTEIN FAMILY: LAG3 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the LAG3 family. FUNCTION: Lymphocyte activation gene 3 protein: Inhibitory receptor on antigen activated T-cells. Delivers inhibitory signals upon binding to ligands, such as FGL1 (By similarity). FGL1 constitutes a major ligand of LAG3 and is responsible for LAG3 T-cell inhibitory function (By similarity). Following TCR engagement, LAG3 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation (By similarity). May inhibit antigen-specific T-cell activation in synergy with PDCD1/PD-1, possibly by acting as a coreceptor for PDCD1/PD-1 (By similarity). Negatively regulates the proliferation, activation, effector function and homeostasis of both CD8(+) and CD4(+) T-cells. Also mediates immune tolerance: constitutively expressed on a subset of regulatory T-cells (Tregs) and contributes to their suppressive function (By similarity). Also acts as a negative regulator of plasmacytoid dendritic cell (pDCs) activation (By similarity). Binds MHC class II (MHC-II); the precise role of MHC-II-binding is however unclear.; FUNCTION: [Secreted lymphocyte activation gene 3 protein]: May function as a ligand for MHC class II (MHC-II) on antigen-presenting cells (APC), promoting APC activation/maturation and driving Th1 immune response. KEYWORDS: 3D-structure;Adaptive immunity;Alternative splicing;Cell membrane;Disulfide bond;Glycoprotein;Immunity;Immunoglobulin domain;Membrane;Reference proteome;Repeat;Secreted;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: [Lymphocyte activation gene 3 protein]: Cell membrane ; Single-pass type I membrane protein.; SUBCELLULAR LOCATION: [Secreted lymphocyte activation gene 3 protein]: Secreted Note=Produced following cleavage of the main chain. " P18851,"PROTEIN NAMES: Guanine nucleotide-binding protein subunit beta PROTEIN FAMILY: WD repeat G protein beta family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the WD repeat G protein beta family. FUNCTION: Implicated in the a- and alpha-factor response pathway. The beta and gamma chains of the putative yeast mating response pathway G protein play a positive role in initiation of the mating response. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction. KEYWORDS: 3D-structure;Pheromone response;Reference proteome;Repeat;Transducer;WD repeat MISCELLANEOUS: Present with 2050 molecules/cell in log phase SD medium." P18891,"PROTEIN NAMES: Fucose-specific lectin (Aleuria aurantia lectin) (AAL) PROTEIN FAMILY: Fungal fucose-specific lectin family ORGANISM: Aleuria aurantia (Orange peel mushroom) SIMILARITY: Belongs to the fungal fucose-specific lectin family. FUNCTION: Lectin that specifically binds to L-fucose. Has strongest preference for the alpha-1,6-fucosylated chain (core fucose) on glycoproteins among alpha-1,2-, alpha-1,3-, alpha-1,4-, and alpha-1,6-fucosylated chains. Might play a role in the differentiation of the fruiting body. Exhibits antifungal activity against Mucor racemosus and thus could act as an antifungal protein in natural ecosystems. KEYWORDS: 3D-structure;Direct protein sequencing;Fruiting body;Lectin;Repeat " P18898,"PROTEIN NAMES: Geranylgeranyl transferase type-1 subunit beta (GGTase-I-beta) (Cell division cycle protein 43) (Geranylgeranyl transferase type I subunit beta) (RAS proteins geranylgeranyltransferase subunit beta) (Type I protein geranyl-geranyltransferase subunit beta) (PGGTase I beta) PROTEIN FAMILY: Protein prenyltransferase subunit beta family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the protein prenyltransferase subunit beta family. FUNCTION: Catalyzes the transfer of a geranyl-geranyl moiety from geranyl-geranyl diphosphate to proteins having the C-terminal sequence Cys-Ile-Ile-Leu or Cys-Val-Leu-Leu. Acts, among other substrates, on Rho1 and Rho2 and CDC42 proteins. Participates in a RAS-like C-terminal modification of proteins involved in nuclear division and bud growth. It is involved in bud positioning and cell polarity. The beta subunit is responsible for isoprenoid and peptide-binding. KEYWORDS: Cytoplasm;Magnesium;Metal-binding;Prenyltransferase;Reference proteome;Repeat;Transferase;Zinc SUBCELLULAR LOCATION: Cytoplasm MISCELLANEOUS: Present with 3940 molecules/cell in log phase SD medium." P19109,"PROTEIN NAMES: ATP-dependent RNA helicase p62 PROTEIN FAMILY: DEAD box helicase family, DDX5/DBP2 subfamily ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the DEAD box helicase family. DDX5/DBP2 subfamily. FUNCTION: As an RNA helicase, unwinds RNA and alters RNA structures through ATP binding and hydrolysis. Involved in multiple cellular processes, including pre-mRNA splicing, alternative splicing, rRNA processing and miRNA processing, as well as transcription regulation (By similarity). Plays a role in innate immunity. Specifically restricts bunyavirus infection, including Rift Valley fever virus (RVFV) or La Crosse virus (LACV), but not vesicular stomatitis virus (VSV), in an interferon- and DROSHA-independent manner. KEYWORDS: Alternative splicing;Antiviral defense;ATP-binding;Cytoplasm;Helicase;Hydrolase;Immunity;mRNA processing;mRNA splicing;Nucleotide-binding;Nucleus;Reference proteome;RNA-binding;RNA-mediated gene silencing;Translation regulation SUBCELLULAR LOCATION: Nucleus Nucleus, nucleolus Cytoplasm, cytosol Note=In the course of bunyavirus infection, relocalizes from the nucleus to the cytosol where it binds viral RNA to antagonize replication. " P19262,"PROTEIN NAMES: Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial (DLST) (2-oxoglutarate dehydrogenase complex component E2) (OGDC-E2) (OGDHC subunit E2) (Alpha-ketoglutarate dehydrogenase subunit E2) (alpha-KGDHC subunit E2) (Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex) PROTEIN FAMILY: 2-oxoacid dehydrogenase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the 2-oxoacid dehydrogenase family. FUNCTION: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3). PATHWAY: Amino-acid degradation; L-lysine degradation via saccharopine pathway; glutaryl-CoA from L-lysine: step 6/6. KEYWORDS: Acyltransferase;Lipoyl;Mitochondrion;Phosphoprotein;Reference proteome;Repeat;Transferase;Transit peptide;Tricarboxylic acid cycle SUBCELLULAR LOCATION: Mitochondrion MISCELLANEOUS: Present with 7970 molecules/cell in log phase SD medium." P19313,"PROTEIN NAMES: Cystatin-S (Cystatin-1) (Protein LM) PROTEIN FAMILY: Cystatin family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the cystatin family. FUNCTION: This protein strongly inhibits papain and ficin, partially inhibits stem bromelain and bovine cathepsin C, but does not inhibit porcine cathepsin B or clostripain. Papain is inhibited non-competitively. KEYWORDS: Direct protein sequencing;Disulfide bond;Protease inhibitor;Reference proteome;Secreted;Signal;Thiol protease inhibitor SUBCELLULAR LOCATION: Secreted. " P19339,"PROTEIN NAMES: Protein sex-lethal ORGANISM: Drosophila melanogaster (Fruit fly) FUNCTION: Sex determination switch protein, which controls sexual development and dosage compensation in females. Sxl protein is only active in females: it is inactive in males throughout development. Acts as a mRNA-binding protein, which specifically binds to a subset of pre-mRNAs and mRNAs and regulates their processing and/or translation. Promotes sexual development by controlling the female-specific alternative splicing of the transformer (tra) pre-mRNA: binds tightly to a characteristic uridine-rich polypyrimidine tract at the non-sex specific 3' splice site in one of the tra introns, preventing the general splicing factor U2AF from binding to this site and forcing it to bind to the female-specific 3' splice site. Acts as an inhibitor of dosage compensation in females by preventing production of msl-2 protein, an essential component of the MSL complex, the complex that mediates X-chromosome dosage compensation. Specifially binds to uridine stretches in both the 5'- and 3'-UTR of msl-2 transcripts. Sxl first acts at the splicing level by promoting retention of an intron in the 5' UTR of msl-2 pre-mRNA. The retained intron contains Sxl-binding sites that are required for subsequent steps of repression: after msl-2 mRNA export into the cytoplasm, Sxl coordinates its translational repression by targeting early steps of translation initiation. Together with how, Sxl also prevents production of msl-2 protein by preventing nuclear export of msl-2 transcripts.; FUNCTION: [Isoform 1]: Embryo-specific product, which is expressed early only in female embryos and specifies female-adult specific splicing. KEYWORDS: 3D-structure;Alternative splicing;Cytoplasm;Differentiation;Nucleus;Reference proteome;Repeat;RNA-binding;Sexual differentiation;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus Cytoplasm " P19397,"PROTEIN NAMES: Leukocyte surface antigen CD53 (Cell surface glycoprotein CD53) (Tetraspanin-25) (Tspan-25) (CD antigen CD53) PROTEIN FAMILY: Tetraspanin (TM4SF) family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the tetraspanin (TM4SF) family. FUNCTION: Structural component of specialized membrane microdomains known as tetraspanin-enriched microdomains (TERMs), which act as platforms for receptor clustering and signaling. Participates thereby in diverse biological functions such as cell signal transduction, adhesion, migration and protein trafficking. Plays a role in the activation of monocytes and B-cells. Acts as an essential regulator of B-cell development by promoting interleukin-7 receptor/IL7R signaling (By similarity). Promotes also in B-cells the BCR signaling by recruiting PKC to the plasma membrane in order to phosphorylate its substrates. Plays an essential role in B- and T-cells homing to lymph nodes by stabilizing L-selectin/SELL cell surface expression (By similarity). Mediates also metabolic and inflammatory functions in hepatocytes and adipose tissue by promoting TNF-alpha and LPS signaling independent of the immune compartment (By similarity). KEYWORDS: 3D-structure;Cell junction;Cell membrane;Glycoprotein;Membrane;Reference proteome;Synapse;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane Cell junction Membrane; Multi-pass membrane protein. Synapse Note=Concentrates in localized microdomains along the plasma membrane at the contact sites between cells of fused myotubes. " P19427,"PROTEIN NAMES: Dermatopontin (22 kDa extracellular matrix protein) (Dermatan sulfate proteoglycan-associated protein 22K) (Tyrosine-rich acidic matrix protein) (TRAMP) PROTEIN FAMILY: Dermatopontin family ORGANISM: Bos taurus (Bovine) SIMILARITY: Belongs to the dermatopontin family. FUNCTION: Seems to mediate adhesion by cell surface integrin binding. May serve as a communication link between the dermal fibroblast cell surface and its extracellular matrix environment. Enhances TGFB1 activity. Inhibits cell proliferation. Accelerates collagen fibril formation, and stabilizes collagen fibrils against low-temperature dissociation. KEYWORDS: Cell adhesion;Direct protein sequencing;Disulfide bond;Extracellular matrix;Pyrrolidone carboxylic acid;Reference proteome;Repeat;Secreted;Signal;Sulfation SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix " P19440,"PROTEIN NAMES: Glutathione hydrolase 1 proenzyme (Gamma-glutamyltransferase 1) (Gamma-glutamyltranspeptidase 1) (GGT 1) (Leukotriene-C4 hydrolase) (CD antigen CD224) [Cleaved into: Glutathione hydrolase 1 heavy chain; Glutathione hydrolase 1 light chain] PROTEIN FAMILY: Gamma-glutamyltransferase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the gamma-glutamyltransferase family. FUNCTION: Cleaves the gamma-glutamyl bond of extracellular glutathione (gamma-Glu-Cys-Gly), glutathione conjugates (such as maresin conjugate (13R)-S-glutathionyl-(14S)-hydroxy-(4Z,7Z,9E,11E,16Z,19Z)-docosahexaenoate, MCTR1) and other gamma-glutamyl compounds (such as leukotriene C4, LTC4). The metabolism of glutathione by GGT1 releases free glutamate and the dipeptide cysteinyl-glycine, which is hydrolyzed to cysteine and glycine by dipeptidases. In the presence of high concentrations of dipeptides and some amino acids, can also catalyze a transpeptidation reaction, transferring the gamma-glutamyl moiety to an acceptor amino acid to form a new gamma-glutamyl compound. Contributes to cysteine homeostasis, glutathione homeostasis and in the conversion of the leukotriene LTC4 to LTD4.; FUNCTION: [Isoform 3]: Seems to be inactive. PATHWAY: Lipid metabolism; leukotriene D4 biosynthesis.; PATHWAY: Sulfur metabolism; glutathione metabolism. KEYWORDS: 3D-structure;Acyltransferase;Alternative promoter usage;Alternative splicing;Cell membrane;Direct protein sequencing;Disease variant;Disulfide bond;Glutathione biosynthesis;Glycoprotein;Hydrolase;Intellectual disability;Lipid metabolism;Membrane;Protease;Reference proteome;Sialic acid;Signal-anchor;Transferase;Transmembrane;Transmembrane helix;Zymogen SUBCELLULAR LOCATION: Cell membrane ; Single-pass type II membrane protein MISCELLANEOUS: Cys-454 was thought to bind the gamma-glutamyl moiety, but mutagenesis of this residue had no effect on activity.; MISCELLANEOUS: Chloride ions bound in the active site cavity may contribute to stabilize the protein fold.; MISCELLANEOUS: [Isoform 1]: Produced by alternative promoter usage.; MISCELLANEOUS: [Isoform 2]: Produced by alternative splicing of isoform 1.; MISCELLANEOUS: [Isoform 3]: Produced by alternative promoter usage." P19474,"PROTEIN NAMES: E3 ubiquitin-protein ligase TRIM21 (52 kDa Ro protein) (52 kDa ribonucleoprotein autoantigen Ro/SS-A) (RING finger protein 81) (Ro(SS-A)) (Sjoegren syndrome type A antigen) (SS-A) (Tripartite motif-containing protein 21) PROTEIN FAMILY: TRIM/RBCC family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the TRIM/RBCC family. FUNCTION: E3 ubiquitin-protein ligase whose activity is dependent on E2 enzymes, UBE2D1, UBE2D2, UBE2E1 and UBE2E2. Forms a ubiquitin ligase complex in cooperation with the E2 UBE2D2 that is used not only for the ubiquitination of USP4 and IKBKB but also for its self-ubiquitination. Component of cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes such as SCF(SKP2)-like complexes. A TRIM21-containing SCF(SKP2)-like complex is shown to mediate ubiquitination of CDKN1B ('Thr-187' phosphorylated-form), thereby promoting its degradation by the proteasome. Monoubiquitinates IKBKB that will negatively regulates Tax-induced NF-kappa-B signaling. Negatively regulates IFN-beta production post-pathogen recognition by catalyzing polyubiquitin-mediated degradation of IRF3. Mediates the ubiquitin-mediated proteasomal degradation of IgG1 heavy chain, which is linked to the VCP-mediated ER-associated degradation (ERAD) pathway. Promotes IRF8 ubiquitination, which enhanced the ability of IRF8 to stimulate cytokine genes transcription in macrophages (By similarity). Plays a role in the regulation of the cell cycle progression. Enhances the decapping activity of DCP2. Exists as a ribonucleoprotein particle present in all mammalian cells studied and composed of a single polypeptide and one of four small RNA molecules. At least two isoforms are present in nucleated and red blood cells, and tissue specific differences in RO/SSA proteins have been identified. The common feature of these proteins is their ability to bind HY RNAs.2. Involved in the regulation of innate immunity and the inflammatory response in response to IFNG/IFN-gamma. Organizes autophagic machinery by serving as a platform for the assembly of ULK1, Beclin 1/BECN1 and ATG8 family members and recognizes specific autophagy targets, thus coordinating target recognition with assembly of the autophagic apparatus and initiation of autophagy. Regulates also autophagy through FIP200/RB1CC1 ubiquitination and subsequent decreased protein stability. Represses the innate antiviral response by facilitating the formation of the NMI-IFI35 complex through 'Lys-63'-linked ubiquitination of NMI. During viral infection, promotes cell pyroptosis by mediating 'Lys-6'-linked ubiquitination of ISG12a/IFI27, facilitating its translocation into the mitochondria and subsequent CASP3 activation. When up-regulated through the IFN/JAK/STAT signaling pathway, promotes 'Lys-27'-linked ubiquitination of MAVS, leading to the recruitment of TBK1 and up-regulation of innate immunity. Mediates 'Lys-63'-linked polyubiquitination of G3BP1 in response to heat shock, leading to stress granule disassembly. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: 3D-structure;Alternative splicing;Cell cycle;Coiled coil;Cytoplasm;Cytoplasmic vesicle;DNA-binding;Host-virus interaction;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Ribonucleoprotein;RNA-binding;Transferase;Ubl conjugation;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cytoplasm Cytoplasmic vesicle, autophagosome Nucleus Cytoplasm, P-body Cytoplasm, Stress granule Note=Enters the nucleus upon exposure to nitric oxide. Localizes to small dot- or rod-like structures in the cytoplasm, called processing bodies (P-bodies) that are located underneath the plasma membrane and also diffusely in the cytoplasm. They are located along the microtubules and are highly motile in cells. Colocalizes with DCP2 in P-bodies. Localizes to stress granules in response to oxidative stress. " P19484,"PROTEIN NAMES: Transcription factor EB (Class E basic helix-loop-helix protein 35) (bHLHe35) PROTEIN FAMILY: MiT/TFE family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the MiT/TFE family. FUNCTION: Transcription factor that acts as a master regulator of lysosomal biogenesis, autophagy, lysosomal exocytosis, lipid catabolism, energy metabolism and immune response. Specifically recognizes and binds E-box sequences (5'-CANNTG-3'); efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. Involved in the cellular response to amino acid availability by acting downstream of MTOR: in the presence of nutrients, TFEB phosphorylation by MTOR promotes its cytosolic retention and subsequent inactivation. Upon starvation or lysosomal stress, inhibition of MTOR induces TFEB dephosphorylation, resulting in nuclear localization and transcription factor activity. Specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression, thereby playing a central role in expression of lysosomal genes. Regulates lysosomal positioning in response to nutrient deprivation by promoting the expression of PIP4P1. Acts as a positive regulator of autophagy by promoting expression of genes involved in autophagy. In association with TFE3, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity (By similarity). Specifically recognizes the gamma-E3 box, a subset of E-boxes, present in the heavy-chain immunoglobulin enhancer. Plays a role in the signal transduction processes required for normal vascularization of the placenta (By similarity). Involved in the immune response to infection by the bacteria S.aureus, S.typhimurium or S.enterica: infection promotes itaconate production, leading to alkylation, resulting in nuclear localization and transcription factor activity. Itaconate-mediated alkylation activates TFEB-dependent lysosomal biogenesis, facilitating the bacteria clearance during the antibacterial innate immune response. In association with ACSS2, promotes the expression of genes involved in lysosome biogenesis and both autophagy upon glucose deprivation. KEYWORDS: 3D-structure;Activator;Adaptive immunity;Alternative splicing;Autophagy;Cytoplasm;DNA-binding;Immunity;Lysosome;Membrane;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation;Ubl conjugation SUBCELLULAR LOCATION: Nucleus Cytoplasm, cytosol Lysosome membrane Note=Mainly present in the cytoplasm. When nutrients are present, recruited to the lysosomal membrane via association with GDP-bound RagC/RRAGC (or RagD/RRAGD): it is then phosphorylated by MTOR. Phosphorylation by MTOR prevents nuclear translocation and activity by promoting interaction with 14-3-3 proteins, such as YWHAZ. Under aberrant lysosomal storage conditions, it translocates from the cytoplasm to the nucleus. The translocation to the nucleus is regulated by ATP13A2. Conversely, inhibition of mTORC1, starvation and lysosomal disruption, promotes dephosphorylation and translocation to the nucleus. Exported from the nucleus in response to nutrient availability. In macrophages, translocates into the nucleus upon live S.enterica infection.; SUBCELLULAR LOCATION: Nucleus Note=(Microbial infection) Following Coxsackievirus B3 infection, full length TFEB and viral protease 3C-mediated cleavage product are translocated from the cytoplasm to the nucleus. " P19525,"PROTEIN NAMES: Interferon-induced, double-stranded RNA-activated protein kinase (Eukaryotic translation initiation factor 2-alpha kinase 2) (eIF-2A protein kinase 2) (Interferon-inducible RNA-dependent protein kinase) (P1/eIF-2A protein kinase) (Protein kinase RNA-activated) (PKR) (Protein kinase R) (Tyrosine-protein kinase EIF2AK2) (p68 kinase) PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family, GCN2 subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily. FUNCTION: IFN-induced dsRNA-dependent serine/threonine-protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (EIF2S1/eIF-2-alpha) and plays a key role in the innate immune response to viral infection. Inhibits viral replication via the integrated stress response (ISR): EIF2S1/eIF-2-alpha phosphorylation in response to viral infection converts EIF2S1/eIF-2-alpha in a global protein synthesis inhibitor, resulting to a shutdown of cellular and viral protein synthesis, while concomitantly initiating the preferential translation of ISR-specific mRNAs, such as the transcriptional activator ATF4. Exerts its antiviral activity on a wide range of DNA and RNA viruses including hepatitis C virus (HCV), hepatitis B virus (HBV), measles virus (MV) and herpes simplex virus 1 (HHV-1). Also involved in the regulation of signal transduction, apoptosis, cell proliferation and differentiation: phosphorylates other substrates including p53/TP53, PPP2R5A, DHX9, ILF3, IRS1 and the HHV-1 viral protein US11. In addition to serine/threonine-protein kinase activity, also has tyrosine-protein kinase activity and phosphorylates CDK1 at 'Tyr-4' upon DNA damage, facilitating its ubiquitination and proteasomal degradation. Either as an adapter protein and/or via its kinase activity, can regulate various signaling pathways (p38 MAP kinase, NF-kappa-B and insulin signaling pathways) and transcription factors (JUN, STAT1, STAT3, IRF1, ATF3) involved in the expression of genes encoding pro-inflammatory cytokines and IFNs. Activates the NF-kappa-B pathway via interaction with IKBKB and TRAF family of proteins and activates the p38 MAP kinase pathway via interaction with MAP2K6. Can act as both a positive and negative regulator of the insulin signaling pathway (ISP). Negatively regulates ISP by inducing the inhibitory phosphorylation of insulin receptor substrate 1 (IRS1) at 'Ser-312' and positively regulates ISP via phosphorylation of PPP2R5A which activates FOXO1, which in turn up-regulates the expression of insulin receptor substrate 2 (IRS2). Can regulate NLRP3 inflammasome assembly and the activation of NLRP3, NLRP1, AIM2 and NLRC4 inflammasomes. Plays a role in the regulation of the cytoskeleton by binding to gelsolin (GSN), sequestering the protein in an inactive conformation away from actin (By similarity). KEYWORDS: 3D-structure;Acetylation;Alternative splicing;Antiviral defense;ATP-binding;Cytoplasm;Direct protein sequencing;Disease variant;Dystonia;Host-virus interaction;Immunity;Innate immunity;Isopeptide bond;Kinase;Magnesium;Nucleotide-binding;Nucleus;Phosphoprotein;Reference proteome;Repeat;RNA-binding;Serine/threonine-protein kinase;Transcription;Transcription regulation;Transferase;Tyrosine-protein kinase;Ubl conjugation SUBCELLULAR LOCATION: Cytoplasm Nucleus Cytoplasm, perinuclear region Note=Nuclear localization is elevated in acute leukemia, myelodysplastic syndrome (MDS), melanoma, breast, colon, prostate and lung cancer patient samples or cell lines as well as neurocytes from advanced Creutzfeldt-Jakob disease patients. " P19532,"PROTEIN NAMES: Transcription factor E3 (Class E basic helix-loop-helix protein 33) (bHLHe33) PROTEIN FAMILY: MiT/TFE family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the MiT/TFE family. FUNCTION: Transcription factor that acts as a master regulator of lysosomal biogenesis and immune response. Specifically recognizes and binds E-box sequences (5'-CANNTG-3'); efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFEB or MITF. Involved in the cellular response to amino acid availability by acting downstream of MTOR: in the presence of nutrients, TFE3 phosphorylation by MTOR promotes its inactivation. Upon starvation or lysosomal stress, inhibition of MTOR induces TFE3 dephosphorylation, resulting in transcription factor activity. Specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression, thereby playing a central role in expression of lysosomal genes. Maintains the pluripotent state of embryonic stem cells by promoting the expression of genes such as ESRRB; mTOR-dependent TFE3 cytosolic retention and inactivation promotes exit from pluripotency (By similarity). Required to maintain the naive pluripotent state of hematopoietic stem cell; mTOR-dependent cytoplasmic retention of TFE3 promotes the exit of hematopoietic stem cell from pluripotency. TFE3 activity is also involved in the inhibition of neuronal progenitor differentiation (By similarity). Acts as a positive regulator of browning of adipose tissue by promoting expression of target genes; mTOR-dependent phosphorylation promotes cytoplasmic retention of TFE3 and inhibits browning of adipose tissue (By similarity). In association with TFEB, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity (By similarity). Specifically recognizes the MUE3 box, a subset of E-boxes, present in the immunoglobulin enhancer. It also binds very well to a USF/MLTF site. Promotes TGF-beta-induced transcription of COL1A2; via its interaction with TSC22D1 at E-boxes in the gene proximal promoter (By similarity). May regulate lysosomal positioning in response to nutrient deprivation by promoting the expression of PIP4P1. KEYWORDS: 3D-structure;Activator;Adaptive immunity;Alternative splicing;Chromosomal rearrangement;Cytoplasm;Disease variant;DNA-binding;Immunity;Intellectual disability;Isopeptide bond;Lysosome;Membrane;Methylation;Nucleus;Phosphoprotein;Proto-oncogene;Reference proteome;Transcription;Transcription regulation;Ubl conjugation SUBCELLULAR LOCATION: Cytoplasm, cytosol Nucleus Lysosome membrane Note=When nutrients are present, recruited to the lysosomal membrane via association with GDP-bound RagC/RRAGC (or RagD/RRAGD): it is then phosphorylated by MTOR. Phosphorylation by MTOR prevents nuclear translocation and promotes ubiquitination and degradation. Conversely, inhibition of mTORC1, starvation and lysosomal disruption, promotes dephosphorylation and translocation to the nucleus. " P19880,"PROTEIN NAMES: AP-1-like transcription factor YAP1 (Phenanthroline resistance protein PAR1) (Pleiotropic drug resistance protein PDR4) PROTEIN FAMILY: BZIP family, YAP subfamily ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the bZIP family. YAP subfamily. FUNCTION: Transcription activator involved in oxidative stress response and redox homeostasis. Regulates the transcription of genes encoding antioxidant enzymes and components of the cellular thiol-reducing pathways, including the thioredoxin system (TRX2, TRR1), the glutaredoxin system (GSH1, GLR1), superoxide dismutase (SOD1, SOD2), glutathione peroxidase (GPX2), and thiol-specific peroxidases (TSA1, AHP1). The induction of some of these genes requires the cooperative action of both, YAP1 and SKN7. Preferentially binds to promoters with the core binding site 5'-TTA[CG]TAA-3'. Activity of the transcription factor is controlled through oxidation of specific cysteine residues resulting in the alteration of its subcellular location. Oxidative stress (as well as carbon stress, but not increased temperature, acidic pH, or ionic stress) induces nuclear accumulation and as a result YAP1 transcriptional activity. Activation by hydrogen peroxide or thiol-reactive chemicals elicit distinct adaptive gene responses. Nuclear export is restored when disulfide bonds are reduced by thioredoxin (TRX2), whose expression is controlled by YAP1, providing a mechanism for negative autoregulation. When overexpressed, YAP1 confers pleiotropic drug-resistance and increases cellular tolerance to cadmium, iron chelators and zinc. KEYWORDS: 3D-structure;Activator;Cadmium resistance;Cytoplasm;Disulfide bond;DNA-binding;Nucleus;Oxidation;Phosphoprotein;Reference proteome;Repeat;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus Cytoplasm Note=Oxidized YAP1 is found predominantly in the nucleus, while reduced YAP1 is continuously exported to the cytoplasm by CRM1/exportin 1. Nuclear import requires the karyopherin PSE1/KAP121 and is independent on YAP1 oxidation state. MISCELLANEOUS: One of 8 closely related fungi-specific YAP proteins (YAP1 to YAP8), which all seem to be transcription activators of the environmental stress response and metabolism control pathways and to have similar but not identical DNA binding specificities.; MISCELLANEOUS: Present with 1600 molecules/cell in log phase SD medium." P19954,"PROTEIN NAMES: Ribosome-binding factor PSRP1, chloroplastic (30S ribosomal protein 1) (CS-S5) (CS5) (Plastid-specific 30S ribosomal protein 1) (PSrp-1) (Ribosomal protein 1) (S22) (Translation factor pY) PROTEIN FAMILY: HPF/YfiA ribosome-associated protein family, Long plastid HPF subfamily ORGANISM: Spinacia oleracea (Spinach) SIMILARITY: Belongs to the HPF/YfiA ribosome-associated protein family. Long plastid HPF subfamily. FUNCTION: Ribosome-binding factor involved in light- and temperature-dependent control of protein synthesis. Interacts with 16S sRNA nucleotides at the A-site and P-site, where it protects the decoding center and inhibits translation by preventing tRNA binding. Stabilizes 70S ribosomes against dissociation. May be recycled by the combined action of ribosome-recycling factor (RRF) and EF-G. KEYWORDS: 3D-structure;Chloroplast;Direct protein sequencing;Plastid;Reference proteome;RNA-binding;rRNA-binding;Transit peptide;Translation regulation SUBCELLULAR LOCATION: Plastid, chloroplast stroma " P19971,"PROTEIN NAMES: Thymidine phosphorylase (TP) (Gliostatin) (Platelet-derived endothelial cell growth factor) (PD-ECGF) (TdRPase) PROTEIN FAMILY: Thymidine/pyrimidine-nucleoside phosphorylase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the thymidine/pyrimidine-nucleoside phosphorylase family. FUNCTION: May have a role in maintaining the integrity of the blood vessels. Has growth promoting activity on endothelial cells, angiogenic activity in vivo and chemotactic activity on endothelial cells in vitro.; FUNCTION: Catalyzes the reversible phosphorolysis of thymidine. The produced molecules are then utilized as carbon and energy sources or in the rescue of pyrimidine bases for nucleotide synthesis. PATHWAY: Pyrimidine metabolism; dTMP biosynthesis via salvage pathway; dTMP from thymine: step 1/2. KEYWORDS: 3D-structure;Alternative splicing;Angiogenesis;Chemotaxis;Developmental protein;Differentiation;Direct protein sequencing;Disease variant;Glycosyltransferase;Growth factor;Neuropathy;Phosphoprotein;Primary mitochondrial disease;Progressive external ophthalmoplegia;Reference proteome;Repeat;Transferase " P20050,PROTEIN NAMES: Meiosis-specific protein HOP1 ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Probable constituent of the synaptonemal complex during meiosis. May interact with RED1. KEYWORDS: 3D-structure;Chromosome;DNA-binding;Meiosis;Metal-binding;Nucleus;Reference proteome;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus. Chromosome. Note=Synapsis of meiotic chromosomes. P20237,"PROTEIN NAMES: Gamma-aminobutyric acid receptor subunit alpha-4 (GABA(A) receptor subunit alpha-4) (GABAAR subunit alpha-4) PROTEIN FAMILY: Ligand-gated ion channel family, Gamma-aminobutyric acid receptor subfamily, GABRA4 sub-subfamily ORGANISM: Bos taurus (Bovine) SIMILARITY: Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRA4 sub-subfamily. FUNCTION: Alpha subunit of the heteropentameric ligand-gated chloride channel gated by gamma-aminobutyric acid (GABA), a major inhibitory neurotransmitter in the brain. GABA-gated chloride channels, also named GABA(A) receptors (GABAAR), consist of five subunits arranged around a central pore and contain GABA active binding site(s) located at the alpha and beta subunit interface(s) (By similarity). When activated by GABA, GABAARs selectively allow the flow of chloride anions across the cell membrane down their electrochemical gradient. GABAARs containing alpha-4 are predominantly extrasynaptic, contributing to tonic inhibition in dentate granule cells and thalamic relay neurons (By similarity). Extrasynaptic alpha-4-containing GABAARs control levels of excitability and network activity (By similarity). GABAARs containing alpha-4 are often found with the delta or gamma-2 subunits, in combination with beta subunits (By similarity). GABAAR containing alpha-4-beta-3-delta subunits can simultaneously bind GABA and histamine where histamine binds at the interface of two neighboring beta subunits, which may be involved in the regulation of sleep and wakefulness (By similarity). KEYWORDS: Cell membrane;Chloride;Chloride channel;Disulfide bond;Glycoprotein;Ion channel;Ion transport;Ligand-gated ion channel;Membrane;Postsynaptic cell membrane;Receptor;Reference proteome;Signal;Synapse;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Postsynaptic cell membrane; Multi-pass membrane protein " P20267,"PROTEIN NAMES: POU domain, class 3, transcription factor 1 (Octamer-binding protein 6) (Oct-6) (Octamer-binding transcription factor 6) (OTF-6) (POU domain transcription factor SCIP) (Tst-1) PROTEIN FAMILY: POU transcription factor family, Class-3 subfamily ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the POU transcription factor family. Class-3 subfamily. FUNCTION: Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') (By similarity). Acts as a transcriptional activator when binding cooperatively with SOX4, SOX11, or SOX12 to gene promoters (By similarity). Acts as a transcriptional repressor of myelin-specific genes. KEYWORDS: Activator;DNA-binding;Homeobox;Nucleus;Reference proteome;Repressor;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " P20350,PROTEIN NAMES: Protein rhomboid (Protein veinlet) PROTEIN FAMILY: Peptidase S54 family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the peptidase S54 family. FUNCTION: Acts early in embryonic development to establish position along the dorsoventral axis and then again later to specify the fate of neuronal precursor cells. Involved in EGF receptor signaling; cleaves Spitz to release the active growth factor. KEYWORDS: Developmental protein;Golgi apparatus;Hydrolase;Membrane;Protease;Reference proteome;Serine protease;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus membrane; Multi-pass membrane protein. P20385,"PROTEIN NAMES: Chorion transcription factor Cf2 ORGANISM: Drosophila melanogaster (Fruit fly) FUNCTION: Transcriptional regulator; binds to the promoter region of Cp15. Also binds to its own promoter, thus having a probable autoregulatory role. KEYWORDS: Alternative splicing;DNA-binding;Metal-binding;Nucleus;Reference proteome;Repeat;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus " P20428,"PROTEIN NAMES: Myogenin ORGANISM: Rattus norvegicus (Rat) FUNCTION: Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation, cell cycle exit and muscle atrophy. Essential for the development of functional embryonic skeletal fiber muscle differentiation. However is dispensable for postnatal skeletal muscle growth; phosphorylation by CAMK2G inhibits its transcriptional activity in respons to muscle activity. Required for the recruitment of the FACT complex to muscle-specific promoter regions, thus promoting gene expression initiation. During terminal myoblast differentiation, plays a role as a strong activator of transcription at loci with an open chromatin structure previously initiated by MYOD1. Together with MYF5 and MYOD1, co-occupies muscle-specific gene promoter core regions during myogenesis. Cooperates also with myocyte-specific enhancer factor MEF2D and BRG1-dependent recruitment of SWI/SNF chromatin-remodeling enzymes to alter chromatin structure at myogenic late gene promoters. Facilitates cell cycle exit during terminal muscle differentiation through the up-regulation of miR-20a expression, which in turn represses genes involved in cell cycle progression. Binds to the E-box containing (E1) promoter region of the miR-20a gene. Plays also a role in preventing reversal of muscle cell differentiation. Contributes to the atrophy-related gene expression in adult denervated muscles. Induces fibroblasts to differentiate into myoblasts. KEYWORDS: Activator;Cell cycle;Developmental protein;Differentiation;DNA-binding;Myogenesis;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus. Note=Recruited to late myogenic gene promoter regulatory sequences with SMARCA4/BRG1/BAF190A and SWI/SNF chromatin-remodeling enzymes to promote chromatin-remodeling and transcription initiation in developing embryos. " P20437,PROTEIN NAMES: G1/S-specific cyclin CLN1 PROTEIN FAMILY: Cyclin family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the cyclin family. FUNCTION: Essential for the control of the cell cycle at the G1/S (start) transition. Interacts with the CDC28 protein kinase to form MPF. KEYWORDS: Cell cycle;Cell division;Cyclin;Reference proteome MISCELLANEOUS: Present with 319 molecules/cell in log phase SD medium. P20439,"PROTEIN NAMES: G2/mitotic-specific cyclin-B PROTEIN FAMILY: Cyclin family, Cyclin AB subfamily ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the cyclin family. Cyclin AB subfamily. FUNCTION: Essential for the control of the cell cycle at the G2/M (mitosis) transition. KEYWORDS: Alternative splicing;Cell cycle;Cell division;Cyclin;Mitosis;Phosphoprotein;Reference proteome " P20582,"PROTEIN NAMES: Anthraniloyl-CoA anthraniloyltransferase (2-heptyl-4(1H)-quinolone synthase PqsD) (PqsD) PROTEIN FAMILY: Thiolase-like superfamily, FabH family ORGANISM: Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) SIMILARITY: Belongs to the thiolase-like superfamily. FabH family. FUNCTION: Required for the biosynthesis of a number of signaling molecules, such as the quinolone signal 2-heptyl-3-hydroxy-4(1H)-quinolone (PQS), 2-heptyl-4-hydroxyquinoline (HHQ) and 2,4-dihydroxyquinoline (DHQ). These molecules are required for normal biofilm formation. Catalyzes the transfer of the anthraniloyl moiety from anthraniloyl-CoA to malonyl-CoA to form 2-aminobenzoylacetyl-CoA. The first step of the reaction is the formation of a covalent anthraniloyl-PqsD intermediate. Next, the short-lived intermediate 3-(2-aminophenyl)-3-oxopropanoyl-CoA is formed. An intramolecular rearrangement of this intermediate can give rise to 2,4-dihydroxyquinoline (DHQ). KEYWORDS: 3D-structure;Acyltransferase;Cytoplasm;Reference proteome;Transferase SUBCELLULAR LOCATION: Cytoplasm " P20693,"PROTEIN NAMES: Low affinity immunoglobulin epsilon Fc receptor (Fc-epsilon-RII) (Lymphocyte IgE receptor) (CD antigen CD23) ORGANISM: Mus musculus (Mouse) FUNCTION: Low-affinity receptor for immunoglobulin E (IgE) and CR2/CD21. Has essential roles in the regulation of IgE production and in the differentiation of B cells. On B cells, initiates IgE-dependent antigen uptake and presentation to T cells. On macrophages, upon IgE binding and antigen cross-linking induces intracellular killing of parasites through activation of L-Arginine-nitric oxide pathway. KEYWORDS: Alternative splicing;Calcium;Cell membrane;Disulfide bond;Glycoprotein;IgE-binding protein;Lectin;Lipoprotein;Membrane;Metal-binding;Palmitate;Proteoglycan;Receptor;Reference proteome;Repeat;Secreted;Signal-anchor;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type II membrane protein Cell membrane ; Lipid-anchor Secreted MISCELLANEOUS: There are two kinds of Fc receptors for IgE, which differ in both structure and function: high affinity receptors on basophils and mast cells and low affinity receptors on lymphocytes and monocytes." P20749,"PROTEIN NAMES: B-cell lymphoma 3 protein (BCL-3) (Proto-oncogene BCL3) ORGANISM: Homo sapiens (Human) FUNCTION: Contributes to the regulation of transcriptional activation of NF-kappa-B target genes. In the cytoplasm, inhibits the nuclear translocation of the NF-kappa-B p50 subunit. In the nucleus, acts as transcriptional activator that promotes transcription of NF-kappa-B target genes. Contributes to the regulation of cell proliferation (By similarity). KEYWORDS: 3D-structure;Activator;ANK repeat;Chromosomal rearrangement;Cytoplasm;Nucleus;Phosphoprotein;Proto-oncogene;Reference proteome;Repeat;Transcription;Transcription regulation;Ubl conjugation SUBCELLULAR LOCATION: Nucleus. Cytoplasm Cytoplasm, perinuclear region Note=Ubiquitination via 'Lys-63'-linked ubiquitin chains is required for nuclear accumulation. " P20792,"PROTEIN NAMES: Cell surface receptor daf-1 (Abnormal dauer formation protein 1) PROTEIN FAMILY: Protein kinase superfamily, TKL Ser/Thr protein kinase family, TGFB receptor subfamily ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily. FUNCTION: Probably involved in a TGF-beta pathway. May be a receptor for TGF-beta-like ligand daf-7. Controls the decision of whether or not larvae enter a developmentally arrested state, known as dauer, in response to environmental conditions. Involved in regulating entry into quiescence triggered by satiety. Involved in sensitivity to CO2 levels. In AWC neurons, acts to promote expression of srsx-3, a member of the GPCR family. KEYWORDS: Alternative splicing;ATP-binding;Developmental protein;Glycoprotein;Kinase;Membrane;Nucleotide-binding;Receptor;Reference proteome;Serine/threonine-protein kinase;Signal;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. " P20849,"PROTEIN NAMES: Collagen alpha-1(IX) chain PROTEIN FAMILY: Fibril-associated collagens with interrupted helices (FACIT) family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the fibril-associated collagens with interrupted helices (FACIT) family. FUNCTION: Structural component of hyaline cartilage and vitreous of the eye. KEYWORDS: 3D-structure;Alternative splicing;Collagen;Deafness;Direct protein sequencing;Disease variant;Disulfide bond;Extracellular matrix;Glycoprotein;Hydroxylation;Metal-binding;Reference proteome;Repeat;Secreted;Signal;Stickler syndrome;Zinc SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix " P20905,"PROTEIN NAMES: 5-hydroxytryptamine receptor 1 (5-HT receptor) (5HT-dro) (Serotonin receptor 1) PROTEIN FAMILY: G-protein coupled receptor 1 family, 5-hydroxytryptamine receptor subfamily ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the G-protein coupled receptor 1 family. 5-hydroxytryptamine receptor subfamily. FUNCTION: G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various alkaloids. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling activates adenylate cyclase activity. KEYWORDS: Cell membrane;Disulfide bond;G-protein coupled receptor;Membrane;Receptor;Reference proteome;Repeat;Transducer;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein " P20932,"PROTEIN NAMES: (S)-mandelate dehydrogenase (MDH) (L(+)-mandelate dehydrogenase) PROTEIN FAMILY: FMN-dependent alpha-hydroxy acid dehydrogenase family ORGANISM: Pseudomonas putida (Arthrobacter siderocapsulatus) SIMILARITY: Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family. FUNCTION: Catalyzes the dehydrogenation of (S)-mandelate to phenylglyoxylate (benzoylformate). Is likely involved in the utilization of mandelate as a sole source of carbon and energy for growth (Probable). Active in vitro with the artificial electron acceptors 2,6-dichlorophenolindophenol (DCPIP) or ferricyanide, but in vivo most likely transfer the electron pair from the reduced flavin to a component of the electron transport chain in the membrane, possibly a quinone. Shows very low activity with oxygen as the electron acceptor, and also with 3-indolelactate and medium chain 2-hydroxyacids as substrates. PATHWAY: Aromatic compound metabolism; (R)-mandelate degradation; benzoate from (R)-mandelate: step 2/4. KEYWORDS: 3D-structure;Aromatic hydrocarbons catabolism;Cell inner membrane;Cell membrane;Direct protein sequencing;Flavoprotein;FMN;Mandelate pathway;Membrane;Oxidoreductase SUBCELLULAR LOCATION: Cell inner membrane " P21163,PROTEIN NAMES: Peptide-N(4)-(N-acetyl-beta-D-glucosaminyl)asparagine amidase F (PNGase F) (Glycopeptide N-glycosidase) (N-glycanase) ORGANISM: Elizabethkingia miricola (Chryseobacterium miricola) FUNCTION: Cleaves an entire glycan from a glycoprotein. Requires that the glycosylated asparagine moiety (reaction 1) be substituted on its amino (R1) and carboxyl (R2) terminus with a polypeptide chain. KEYWORDS: 3D-structure;Direct protein sequencing;Disulfide bond;Hydrolase;Signal MISCELLANEOUS: PNGase F binds primarily to the polypeptide chain around the glycosamine junction including the inner di-N-acetylchitobiose core on the carbohydrate moiety. P21182,"PROTEIN NAMES: S-adenosylmethionine decarboxylase proenzyme (AdoMetDC) (SAMDC) [Cleaved into: S-adenosylmethionine decarboxylase alpha chain; S-adenosylmethionine decarboxylase beta chain] PROTEIN FAMILY: Eukaryotic AdoMetDC family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the eukaryotic AdoMetDC family. FUNCTION: Catalyzes the decarboxylation of S-adenosylmethionine, a key step in the biosynthetic pathway for spermidine and spermine. It is essential for normal growth, sporulation, and maintenance of ds-RNA virus. PATHWAY: Amine and polyamine biosynthesis; S-adenosylmethioninamine biosynthesis; S-adenosylmethioninamine from S-adenosyl-L-methionine: step 1/1. KEYWORDS: Autocatalytic cleavage;Decarboxylase;Direct protein sequencing;Lyase;Polyamine biosynthesis;Pyruvate;Reference proteome;S-adenosyl-L-methionine;Schiff base;Spermidine biosynthesis;Zymogen MISCELLANEOUS: Present with 7060 molecules/cell in log phase SD medium." P21242,"PROTEIN NAMES: Probable proteasome subunit alpha type-7 (Macropain subunit C1) (Multicatalytic endopeptidase complex subunit C1) (Proteasome component C1) (Proteinase YSCE subunit 1) PROTEIN FAMILY: Peptidase T1A family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the peptidase T1A family. FUNCTION: The proteasome degrades poly-ubiquitinated proteins in the cytoplasm and in the nucleus. It is essential for the regulated turnover of proteins and for the removal of misfolded proteins. The proteasome is a multicatalytic proteinase complex that is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. It has an ATP-dependent proteolytic activity. KEYWORDS: 3D-structure;Acetylation;Cytoplasm;Direct protein sequencing;Nucleus;Proteasome;Reference proteome SUBCELLULAR LOCATION: Cytoplasm. Nucleus. MISCELLANEOUS: Present with 12000 molecules/cell in log phase SD medium." P21269,"PROTEIN NAMES: CCA tRNA nucleotidyltransferase, mitochondrial (CCA-adding enzyme) (tRNA CCA-pyrophosphorylase) (tRNA adenylyltransferase) (tRNA nucleotidyltransferase) PROTEIN FAMILY: TRNA nucleotidyltransferase/poly(A) polymerase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. FUNCTION: Nucleotidyltransferase that catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs, which is necessary for the attachment of amino acids to the 3' terminus of tRNA molecules, using CTP and ATP as substrates. tRNA 3'-terminal CCA addition is required both for tRNA processing and repair (By similarity). Also involved in tRNA surveillance by mediating tandem CCA addition to generate a CCACCA at the 3' terminus of unstable tRNAs (By similarity). While stable tRNAs receive only 3'-terminal CCA, unstable tRNAs are marked with CCACCA and rapidly degraded (By similarity). The structural flexibility of RNA controls the choice between CCA versus CCACCA addition: following the first CCA addition cycle, nucleotide-binding to the active site triggers a clockwise screw motion, producing torque on the RNA (By similarity). This ejects stable RNAs, whereas unstable RNAs are refolded while bound to the enzyme and subjected to a second CCA catalytic cycle (By similarity). KEYWORDS: Alternative initiation;ATP-binding;Cytoplasm;Direct protein sequencing;Mitochondrion;Nucleotide-binding;Nucleotidyltransferase;Nucleus;Reference proteome;RNA-binding;Transferase;Transit peptide SUBCELLULAR LOCATION: [Isoform Mitochondrial]: Mitochondrion.; SUBCELLULAR LOCATION: [Isoform Cytoplasmic+nuclear 1]: Cytoplasm Nucleus.; SUBCELLULAR LOCATION: [Isoform Cytoplasmic+nuclear 2]: Cytoplasm Nucleus MISCELLANEOUS: Present with 13500 molecules/cell in log phase SD medium.; MISCELLANEOUS: [Isoform Cytoplasmic+nuclear 1]: Produced by alternative initiation at Met-10 of isoform Mitochondrial.; MISCELLANEOUS: [Isoform Cytoplasmic+nuclear 2]: Produced by alternative initiation at Met-18 of isoform Mitochondrial." P21327,"PROTEIN NAMES: Inositol polyphosphate 1-phosphatase (IPP) (IPPase) PROTEIN FAMILY: Inositol monophosphatase superfamily ORGANISM: Bos taurus (Bovine) SIMILARITY: Belongs to the inositol monophosphatase superfamily. FUNCTION: Mg(2+)-dependent phosphatase that catalyzes the hydrolysis of the 1-position phosphate from inositol 1,4-bisphosphate and inositol 1,3,4-trisphosphate and participates in inositol phosphate metabolism. PATHWAY: Signal transduction; phosphatidylinositol signaling pathway. KEYWORDS: 3D-structure;Direct protein sequencing;Hydrolase;Lithium;Magnesium;Metal-binding;Phosphoprotein;Reference proteome " P21373,"PROTEIN NAMES: NAD(+) kinase (Unknown transcript 1 protein) PROTEIN FAMILY: NAD kinase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the NAD kinase family. FUNCTION: Specifically phosphorylates NAD in the presence of ATP, dATP, or CTP as phosphoryl donors. KEYWORDS: ATP-binding;Kinase;NAD;NADP;Nucleotide-binding;Phosphoprotein;Reference proteome;Transferase MISCELLANEOUS: Present with 5040 molecules/cell in log phase SD medium." P21374,"PROTEIN NAMES: Pre-mRNA-splicing factor ISY1 (Interactor of SYF1) (PRP19-associated complex protein 30) PROTEIN FAMILY: ISY1 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the ISY1 family. FUNCTION: Involved in pre-mRNA splicing and cell cycle control. As a component of the NTC complex (or PRP19-associated complex), associates to the spliceosome to mediate conformational rearrangement or to stabilize the structure of the spliceosome after U4 snRNA dissociation, which leads to spliceosome maturation. The cell cycle arrest of SYF2 defective cells may be due to the inefficient splicing of TUB1. Also involved in DNA repair. KEYWORDS: 3D-structure;Cytoplasm;mRNA processing;mRNA splicing;Nucleus;Reference proteome;Spliceosome SUBCELLULAR LOCATION: Cytoplasm Nucleus MISCELLANEOUS: Present with 1990 molecules/cell in log phase SD medium." P21394,"PROTEIN NAMES: Xylene/toluene monooxygenase electron transfer component XylA [Includes: Ferredoxin; Ferredoxin--NAD(+) reductase ] PROTEIN FAMILY: Bacterial ring-hydroxylating dioxygenase ferredoxin reductase family ORGANISM: Pseudomonas putida (Arthrobacter siderocapsulatus) SIMILARITY: Belongs to the bacterial ring-hydroxylating dioxygenase ferredoxin reductase family. FUNCTION: Component of a monooxygenase that catalyzes the first step in the degradation of xylenes and toluenes. XylA is responsible for the transport of electrons from the electron donor NADH to the terminal hydroxylase component, XylM. KEYWORDS: 2Fe-2S;Aromatic hydrocarbons catabolism;Cell inner membrane;Cell membrane;Direct protein sequencing;FAD;Flavoprotein;Iron;Iron-sulfur;Membrane;Metal-binding;NAD;Oxidoreductase;Plasmid SUBCELLULAR LOCATION: Cell inner membrane ; Peripheral membrane protein " P21397,"PROTEIN NAMES: Amine oxidase [flavin-containing] A (Monoamine oxidase type A) (MAO-A) PROTEIN FAMILY: Flavin monoamine oxidase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the flavin monoamine oxidase family. FUNCTION: Catalyzes the oxidative deamination of primary and some secondary amine such as neurotransmitters, with concomitant reduction of oxygen to hydrogen peroxide and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. Preferentially oxidizes serotonin. Also catalyzes the oxidative deamination of kynuramine to 3-(2-aminophenyl)-3-oxopropanal that can spontaneously condense to 4-hydroxyquinoline (By similarity). KEYWORDS: 3D-structure;Acetylation;Alternative splicing;Catecholamine metabolism;Direct protein sequencing;Disease variant;FAD;Flavoprotein;Intellectual disability;Membrane;Mitochondrion;Mitochondrion outer membrane;Neurotransmitter degradation;Oxidoreductase;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Mitochondrion outer membrane ; Single-pass type IV membrane protein ; Cytoplasmic side " P21507,"PROTEIN NAMES: ATP-dependent RNA helicase SrmB PROTEIN FAMILY: DEAD box helicase family, SrmB subfamily ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the DEAD box helicase family. SrmB subfamily. FUNCTION: DEAD-box RNA helicase involved in the assembly of the 50S ribosomal subunit at low temperature. Exhibits RNA-stimulated ATP hydrolysis and RNA unwinding activity. Acts before DeaD. KEYWORDS: 3D-structure;ATP-binding;Cytoplasm;Helicase;Hydrolase;Nucleotide-binding;Reference proteome;Ribosome biogenesis;RNA-binding SUBCELLULAR LOCATION: Cytoplasm MISCELLANEOUS: Overexpression suppresses a mutant defective in 50S ribosomal subunit assembly." P21514,"PROTEIN NAMES: Cyclic di-GMP phosphodiesterase PdeL ORGANISM: Escherichia coli (strain K12) FUNCTION: Acts both as an enzyme and as a c-di-GMP sensor to couple transcriptional activity to the c-di-GMP status of the cell. Phosphodiesterase (PDE) that catalyzes the hydrolysis of cyclic-di-GMP (c-di-GMP) to 5'-pGpG. Also acts as a transcription factor to control its own expression. KEYWORDS: 3D-structure;c-di-GMP;DNA-binding;Hydrolase;Magnesium;Manganese;Metal-binding;Reference proteome;Transcription;Transcription regulation MISCELLANEOUS: An overexpressed EAL domain (residues 77-362) has similar KM, Vmax, pH and metal dependence and subunit composition as the full-length protein." P21541,PROTEIN NAMES: Early embryogenesis protein zyg-11 PROTEIN FAMILY: Zyg-11 family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the zyg-11 family. FUNCTION: Acts as a target recruitment subunit in the E3 ubiquitin ligase complex zyg-11-cul-2-elc-1. Required for metaphase to anaphase transition and M phase exit at meiosis II. Regulates polarity establishment. KEYWORDS: Developmental protein;Meiosis;Reference proteome;Ubl conjugation pathway P21576,"PROTEIN NAMES: Vacuolar protein sorting-associated protein 1 PROTEIN FAMILY: TRAFAC class dynamin-like GTPase superfamily, Dynamin/Fzo/YdjA family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family. FUNCTION: Essential for protein sorting in meiotic cell division of S.cerevisiae; it binds microtubules. Could also be involved in microtubule-associated motility. Necessary for membrane protein retention in a late Golgi compartment. Interacts with the MVP1 protein. KEYWORDS: Cell cycle;Cell division;Cytoplasm;Cytoskeleton;GTP-binding;Meiosis;Microtubule;Motor protein;Nucleotide-binding;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton MISCELLANEOUS: Present with 5960 molecules/cell in log phase SD medium." P21631,"PROTEIN NAMES: Uroporphyrinogen-III C-methyltransferase (Urogen III methylase) (S-adenosyl-L-methionine:uroporphyrinogen III methyltransferase) (SUMT) (Uroporphyrinogen III methylase) (UROM) PROTEIN FAMILY: Precorrin methyltransferase family ORGANISM: Sinorhizobium sp SIMILARITY: Belongs to the precorrin methyltransferase family. FUNCTION: Catalyzes the two successive C-2 and C-7 methylation reactions involved in the conversion of uroporphyrinogen III to precorrin-2 via the intermediate formation of precorrin-1. It is a step in the biosynthesis of both cobalamin (vitamin B12) and siroheme. Neither uroporphyrin III nor the chlorin (factor I) is a substrate of SUMT. PATHWAY: Cofactor biosynthesis; adenosylcobalamin biosynthesis; precorrin-2 from uroporphyrinogen III: step 1/1.; PATHWAY: Porphyrin-containing compound metabolism; siroheme biosynthesis; precorrin-2 from uroporphyrinogen III: step 1/1. KEYWORDS: 3D-structure;Cobalamin biosynthesis;Direct protein sequencing;Methyltransferase;Porphyrin biosynthesis;S-adenosyl-L-methionine;Transferase MISCELLANEOUS: Uroporphyrinogen III is a key intermediate because of its position at the branch point of two divergent pathways. One pathway leads to protoheme, and the other one is the cobalamin and siroheme pathway." P21775,"PROTEIN NAMES: 3-ketoacyl-CoA thiolase A, peroxisomal (Acetyl-CoA C-myristoyltransferase) (Acetyl-CoA acyltransferase A) (Beta-ketothiolase A) (Peroxisomal 3-oxoacyl-CoA thiolase A) (Thiolase A) PROTEIN FAMILY: Thiolase-like superfamily, Thiolase family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the thiolase-like superfamily. Thiolase family. FUNCTION: Responsible for the thiolytic cleavage of straight chain 3-keto fatty acyl-CoAs (3-oxoacyl-CoAs). Plays an important role in fatty acid peroxisomal beta-oxidation. Catalyzes the cleavage of short, medium, long, and very long straight chain 3-oxoacyl-CoAs. Medium chain straight 3-oxoacyl-CoAs are preferred substrates. PATHWAY: Lipid metabolism; peroxisomal fatty acid beta-oxidation. KEYWORDS: Acetylation;Acyltransferase;Alternative splicing;Direct protein sequencing;Fatty acid metabolism;Lipid metabolism;Peroxisome;Reference proteome;Transferase;Transit peptide SUBCELLULAR LOCATION: Peroxisome Note=Transported into peroxisomes following association with PEX7. " P21825,"PROTEIN NAMES: Translocation protein SEC62 (Sec62/63 complex 30 kDa subunit) PROTEIN FAMILY: SEC62 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the SEC62 family. FUNCTION: Acts as a component of the Sec62/63 complex which is involved in SRP-independent post-translational translocation across the endoplasmic reticulum (ER) and functions together with the Sec61 complex and KAR2 in a channel-forming translocon complex. In an initial step, the signal sequence seems to bind simultaneously to SEC61 and SEC62. SEC62 and SEC63 are required for interactions between SEC61 and translocating polypeptides. SEC62 may affect SEC1-polypeptide interactions by increasing the affinity of targeting pathways for SEC61 and/or by modifying SEC61 to allow more efficient polypeptide interaction. A cycle of assembly and disassembly of Sec62/63 complex from SEC61 may govern the activity of the translocon. SEC62 is essential for cell growth. KEYWORDS: 3D-structure;Acetylation;Endoplasmic reticulum;Membrane;Protein transport;Reference proteome;Translocation;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass membrane protein. MISCELLANEOUS: Present with 16500 molecules/cell in log phase SD medium." P21827,PROTEIN NAMES: Guanine nucleotide exchange factor SRM1 (Pheromone response pathway component SRM1) (Pre-mRNA-processing protein 20) (Regulator of chromosome condensation) (Suppressor of receptor mutations 1) (mRNA transport protein 1) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Guanine nucleotide exchange factor that promotes the exchange of GSP1/GSP2-bound GDP by GTP and controls RNA metabolism and transport. Involved in yeast pheromone response pathway and in mRNA metabolism. Involved in nuclear pore complex (NPC) assembly and required for mRNA and ribosome nuclear export. Binds chromatin and is involved NPC-mediated transcriptional control. KEYWORDS: 3D-structure;Cell cycle;Cell division;Guanine-nucleotide releasing factor;Mitosis;Nucleus;Pheromone response;Phosphoprotein;Reference proteome;Repeat;Transport SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: Present with 12100 molecules/cell in log phase SD medium. P21902,"PROTEIN NAMES: Proclotting enzyme [Cleaved into: Proclotting enzyme light chain; Proclotting enzyme heavy chain] PROTEIN FAMILY: Peptidase S1 family, CLIP subfamily ORGANISM: Tachypleus tridentatus (Japanese horseshoe crab) SIMILARITY: Belongs to the peptidase S1 family. CLIP subfamily. FUNCTION: This enzyme is closely associated with an endotoxin-sensitive hemolymph coagulation system in limulus. Its active form catalyzes the conversion of coagulogen to insoluble coagulin gel. KEYWORDS: Calcium;Cleavage on pair of basic residues;Cytoplasmic vesicle;Direct protein sequencing;Disulfide bond;Glycoprotein;Hemolymph clotting;Hydrolase;Metal-binding;Protease;Pyrrolidone carboxylic acid;Secreted;Serine protease;Signal;Zymogen SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle Secreted Note=Secreted in hemolymph probably upon bacterial lipopolysaccharide (LPS) stimulation. " P21917,"PROTEIN NAMES: D(4) dopamine receptor (D(2C) dopamine receptor) (Dopamine D4 receptor) PROTEIN FAMILY: G-protein coupled receptor 1 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the G-protein coupled receptor 1 family. FUNCTION: Dopamine receptor responsible for neuronal signaling in the mesolimbic system of the brain, an area of the brain that regulates emotion and complex behavior. Activated by dopamine, but also by epinephrine and norepinephrine, and by numerous synthetic agonists and drugs. Agonist binding triggers signaling via G proteins that inhibit adenylyl cyclase. Modulates the circadian rhythm of contrast sensitivity by regulating the rhythmic expression of NPAS2 in the retinal ganglion cells (By similarity). KEYWORDS: 3D-structure;Biological rhythms;Cell membrane;Disulfide bond;G-protein coupled receptor;Glycoprotein;Lipoprotein;Membrane;Metal-binding;Palmitate;Receptor;Reference proteome;Repeat;Sodium;Transducer;Transmembrane;Transmembrane helix;Ubl conjugation SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein " P21965,"PROTEIN NAMES: Protein kinase MCK1 (Meiosis and centromere regulatory kinase) PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. FUNCTION: May be an autophosphorylating tyrosine kinase, a bifunctional (serine/tyrosine-specific) protein kinase, or a serine kinase that is a substrate for an associated tyrosine kinase. MCK1 is a transcriptional activator of IME1, it stimulates spore maturation, and play a positive regulatory role in both mitotic centromere function and activation of early meiotic gene expression. KEYWORDS: Acetylation;ATP-binding;Direct protein sequencing;Kinase;Nucleotide-binding;Phosphoprotein;Reference proteome;Serine/threonine-protein kinase;Transferase MISCELLANEOUS: Present with 396 molecules/cell in log phase SD medium." P22133,"PROTEIN NAMES: Malate dehydrogenase, cytoplasmic PROTEIN FAMILY: LDH/MDH superfamily, MDH type 1 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the LDH/MDH superfamily. MDH type 1 family. FUNCTION: The isoenzyme MDH2 may function primarily in the glyoxylate cycle. KEYWORDS: Cytoplasm;Direct protein sequencing;NAD;Oxidoreductase;Phosphoprotein;Reference proteome;Tricarboxylic acid cycle SUBCELLULAR LOCATION: Cytoplasm. MISCELLANEOUS: Yeast contains at least 3 malate dehydrogenase isoenzymes: a mitochondrial (MDH1), a cytoplasmic (MDH2) and a peroxisomal (MDH3).; MISCELLANEOUS: Present with 5260 molecules/cell in log phase SD medium." P22134,PROTEIN NAMES: DNA-3-methyladenine glycosylase (3-methyladenine DNA glycosidase) (3MEA DNA glycosylase) PROTEIN FAMILY: Alkylbase DNA glycosidase AlkA family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the alkylbase DNA glycosidase AlkA family. FUNCTION: Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine or 7-methyladenine from the damaged DNA polymer formed by alkylation lesions. KEYWORDS: Direct protein sequencing;DNA damage;DNA repair;Hydrolase;Nucleus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Nucleus P22143,"PROTEIN NAMES: Cyanamide hydratase (Urea hydro-lyase) PROTEIN FAMILY: Cyanamide dehydrase family ORGANISM: Albifimbria verrucaria (Myrothecium leaf spot and pod blight fungus) (Myrothecium verrucaria) SIMILARITY: Belongs to the cyanamide dehydrase family. FUNCTION: Cyanamide hydratase involved in the detoxification and/or utilization of cyanamide, a toxic nitrile compound distributed widely in the environment. KEYWORDS: Direct protein sequencing;Lyase;Zinc MISCELLANEOUS: This enzyme is highly specific for cyanamide." P22146,"PROTEIN NAMES: 1,3-beta-glucanosyltransferase GAS1 (Glycolipid-anchored surface protein 1) (Glycoprotein GP115) PROTEIN FAMILY: Glycosyl hydrolase 72 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the glycosyl hydrolase 72 family. FUNCTION: Splits internally a 1,3-beta-glucan molecule and transfers the newly generated reducing end (the donor) to the non-reducing end of another 1,3-beta-glucan molecule (the acceptor) forming a 1,3-beta linkage, resulting in the elongation of 1,3-beta-glucan chains in the cell wall. Involved in cell wall biosynthesis and morphogenesis. KEYWORDS: Cell membrane;Cell wall;Cell wall biogenesis/degradation;Direct protein sequencing;Disulfide bond;Glycoprotein;GPI-anchor;Lipoprotein;Membrane;Reference proteome;Secreted;Signal;Transferase SUBCELLULAR LOCATION: Cell membrane ; Lipid-anchor, GPI-anchor Secreted, cell wall Note=Identified as GPI-anchored plasma membrane protein (GPI-PMP) as well as covalently-linked GPI-modified cell wall protein (GPI-CWP) in the outer cell wall layer. MISCELLANEOUS: Present with 11000 wall-bound molecules/cell in log phase YPD medium." P22149,"PROTEIN NAMES: Iron-regulated transcriptional activator AFT1 (Activator of iron transcription protein 1) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Transcription factor that activates the genes for FRE1, FRE2 and FET3 in response to iron deprivationand thereby plays a central role in iron homeostasis. Also required for the expression of LSO1. Recognizes the consensus iron-responsive element (Fe-RE) sequence 5'-CACCC-3' in the promoters of target genes (By similarity). Iron could interact directly with AFT1 and inhibits its activity. In high iron condition, the presence of Fe(2+) or [2Fe-2S] cluster leads to dimerization, which in turn leads to a decrease in DNA affinity (By similarity). KEYWORDS: Activator;Iron;Metal-binding;Nucleus;Reference proteome;Transcription;Transcription regulation;Zinc SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: Present with 2730 molecules/cell in log phase SD medium." P22192,"PROTEIN NAMES: Double-strand-specific pac1 ribonuclease (Protein hcs) ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) FUNCTION: Digests double-stranded RNA. Converts long double-stranded RNAs into short oligonucleotides, leaving 5'-phosphates on their cleavage products. Probably inhibits mating and meiosis by degrading a specific mRNA required for sexual development. KEYWORDS: Endonuclease;Hydrolase;Magnesium;Meiosis;Metal-binding;Nuclease;Phosphoprotein;Reference proteome;RNA-binding " P22193,PROTEIN NAMES: DNA damage checkpoint control protein rad1 PROTEIN FAMILY: Rad1 family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the rad1 family. FUNCTION: Recombinational reparation of DNA damages induced by UV and gamma radiation. KEYWORDS: DNA damage;DNA repair;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus P22270,"PROTEIN NAMES: Tyramine/octopamine receptor (Tyr/Oct-Dro) PROTEIN FAMILY: G-protein coupled receptor 1 family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the G-protein coupled receptor 1 family. FUNCTION: Receptor for both octopamine and tyramine, invertebrate neurotransmitters, and neuromodulators. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. The rank order of potency for agonists is tyramine > octopamine > dopamine > epinephrine > norepinephrine > serotonin > histamine. For antagonists, the rank order is yohimbine > chlorpromazine > phentolamine > mianserine > cyproheptadine > dihydroergotamine > clonidine > synephrine. Tyramine has a functional role in the olfactory system as a neurotransmitter or a neuromodulator. KEYWORDS: Cell membrane;Disulfide bond;G-protein coupled receptor;Glycoprotein;Membrane;Receptor;Reference proteome;Signal;Transducer;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. " P22300,"PROTEIN NAMES: Gamma-aminobutyric acid receptor subunit gamma-2 (GABA(A) receptor subunit gamma-2) (GABAAR subunit gamma-2) PROTEIN FAMILY: Ligand-gated ion channel family, Gamma-aminobutyric acid receptor subfamily, GABRG2 sub-subfamily ORGANISM: Bos taurus (Bovine) SIMILARITY: Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRG2 sub-subfamily. FUNCTION: Gamma subunit of the heteropentameric ligand-gated chloride channel gated by gamma-aminobutyric acid (GABA), a major inhibitory neurotransmitter in the brain. GABA-gated chloride channels, also named GABA(A) receptors (GABAAR), consist of five subunits arranged around a central pore and contain GABA active binding site(s) located at the alpha and beta subunit interface(s). When activated by GABA, GABAARs selectively allow the flow of chloride anions across the cell membrane down their electrochemical gradient (By similarity). Gamma-2/GABRG2-containing GABAARs are found at both synaptic and extrasynaptic sites (By similarity). Chloride influx into the postsynaptic neuron following GABAAR opening decreases the neuron ability to generate a new action potential, thereby reducing nerve transmission (By similarity). GABAARs containing alpha-1 and beta-2 or -3 subunits exhibit synaptogenic activity; the gamma-2 subunit being necessary but not sufficient to induce rapid synaptic contacts formation (By similarity). Extrasynaptic gamma-2-containing receptors contribute to the tonic GABAergic inhibition (By similarity). GABAARs function also as histamine receptor where histamine binds at the interface of two neighboring beta subunits and potentiates GABA response in a gamma-2 subunit-controlled manner (By similarity). KEYWORDS: Alternative splicing;Cell membrane;Cell projection;Chloride;Chloride channel;Cytoplasmic vesicle;Disulfide bond;Glycoprotein;Ion channel;Ion transport;Lipoprotein;Membrane;Palmitate;Phosphoprotein;Postsynaptic cell membrane;Reference proteome;Signal;Synapse;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Postsynaptic cell membrane ; Multi-pass membrane protein Cell membrane ; Multi-pass membrane protein Cell projection, dendrite Cytoplasmic vesicle membrane " P22336,"PROTEIN NAMES: Replication factor A protein 1 (RF-A protein 1) (DNA-binding protein BUF2) (Replication protein A 69 kDa DNA-binding subunit) (Single-stranded DNA-binding protein) PROTEIN FAMILY: Replication factor A protein 1 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the replication factor A protein 1 family. FUNCTION: As part of the replication protein A (RPA/RP-A), a single-stranded DNA-binding heterotrimeric complex, may play an essential role in DNA replication, recombination and repair. Binds and stabilizes single-stranded DNA intermediates, preventing complementary DNA reannealing and recruiting different proteins involved in DNA metabolism. Binds to single-stranded sequences participating in DNA replication in addition to those mediating transcriptional repression (URS1) and activation (CAR1). Stimulates the activity of a cognate strand exchange protein (SEP1). It cooperates with T-AG and DNA topoisomerase I to unwind template DNA containing the simian virus 40 origin of DNA replication. KEYWORDS: 3D-structure;Acetylation;Direct protein sequencing;DNA replication;DNA-binding;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus. MISCELLANEOUS: Present with 4100 molecules/cell in log phase SD medium." P22413,"PROTEIN NAMES: Ectonucleotide pyrophosphatase/phosphodiesterase family member 1 (E-NPP 1) (Membrane component chromosome 6 surface marker 1) (Phosphodiesterase I/nucleotide pyrophosphatase 1) (Plasma-cell membrane glycoprotein PC-1) [Cleaved into: Ectonucleotide pyrophosphatase/phosphodiesterase family member 1, secreted form] [Includes: Alkaline phosphodiesterase I ; Nucleotide pyrophosphatase (NPPase) (Nucleotide diphosphatase)] PROTEIN FAMILY: Nucleotide pyrophosphatase/phosphodiesterase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the nucleotide pyrophosphatase/phosphodiesterase family. FUNCTION: Nucleotide pyrophosphatase that generates diphosphate (PPi) and functions in bone mineralization and soft tissue calcification by regulating pyrophosphate levels (By similarity). PPi inhibits bone mineralization and soft tissue calcification by binding to nascent hydroxyapatite crystals, thereby preventing further growth of these crystals. Preferentially hydrolyzes ATP, but can also hydrolyze other nucleoside 5' triphosphates such as GTP, CTP and UTP to their corresponding monophosphates with release of pyrophosphate, as well as diadenosine polyphosphates, and also 3',5'-cAMP to AMP. May also be involved in the regulation of the availability of nucleotide sugars in the endoplasmic reticulum and Golgi, and the regulation of purinergic signaling. Inhibits ectopic joint calcification and maintains articular chondrocytes by repressing hedgehog signaling; it is however unclear whether hedgehog inhibition is direct or indirect (By similarity). Appears to modulate insulin sensitivity and function. Also involved in melanogenesis. Also able to hydrolyze 2',3'-cGAMP (cyclic GMP-AMP), a second messenger that activates TMEM173/STING and triggers type-I interferon production. 2',3'-cGAMP degradation takes place in the lumen or extracellular space, and not in the cytosol where it is produced; the role of 2',3'-cGAMP hydrolysis is therefore unclear. Not able to hydrolyze the 2',3'-cGAMP linkage isomer 3'-3'-cGAMP. KEYWORDS: 3D-structure;Biomineralization;Calcium;Cell membrane;Diabetes mellitus;Direct protein sequencing;Disease variant;Disulfide bond;Glycoprotein;Hydrolase;Membrane;Metal-binding;Obesity;Phosphoprotein;Reference proteome;Repeat;Secreted;Signal-anchor;Transmembrane;Transmembrane helix;Zinc SUBCELLULAR LOCATION: [Ectonucleotide pyrophosphatase/phosphodiesterase family member 1]: Cell membrane ; Single-pass type II membrane protein. Basolateral cell membrane ; Single-pass type II membrane protein. Note=Targeted to the basolateral membrane in polarized epithelial cells and in hepatocytes, and to matrix vesicles in osteoblasts. In bile duct cells and cancer cells, located to the apical cytoplasmic side.; SUBCELLULAR LOCATION: [Ectonucleotide pyrophosphatase/phosphodiesterase family member 1, secreted form]: Secreted Note=Secreted following proteolytic cleavage. " P22414,"PROTEIN NAMES: Peroxisomal hydratase-dehydrogenase-epimerase (HDE) (Multifunctional beta-oxidation protein) (MFP) [Includes: 2-enoyl-CoA hydratase ; (3R)-3-hydroxyacyl-CoA dehydrogenase ] PROTEIN FAMILY: Short-chain dehydrogenases/reductases (SDR) family ORGANISM: Candida tropicalis (Yeast) SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FUNCTION: Second trifunctional enzyme acting on the beta-oxidation pathway for fatty acids, possessing hydratase-dehydrogenase-epimerase activities. Converts trans-2-enoyl-CoA via D-3-hydroxyacyl-CoA to 3-ketoacyl-CoA. PATHWAY: Lipid metabolism; fatty acid beta-oxidation. KEYWORDS: 3D-structure;Fatty acid metabolism;Isomerase;Lipid metabolism;Lyase;Multifunctional enzyme;NAD;NADP;Oxidoreductase;Peroxisome;Repeat SUBCELLULAR LOCATION: Peroxisome. " P22454,"PROTEIN NAMES: Guanine nucleotide-binding protein alpha-2 subunit PROTEIN FAMILY: G-alpha family, G(q) subfamily ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the G-alpha family. G(q) subfamily. FUNCTION: Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Involved in behavioral responses to P.aeruginosa by controlling the expression of daf-7, a member of the TGF-beta family, in ASJ sensory neurons. KEYWORDS: GTP-binding;Lipoprotein;Magnesium;Metal-binding;Myristate;Nucleotide-binding;Palmitate;Reference proteome;Transducer " P22517,"PROTEIN NAMES: Calcium/calmodulin-dependent protein kinase II PROTEIN FAMILY: Protein kinase superfamily, CAMK Ser/Thr protein kinase family, CaMK subfamily ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily. FUNCTION: Important in cellular regulation. KEYWORDS: ATP-binding;Calmodulin-binding;Kinase;Nucleotide-binding;Phosphoprotein;Reference proteome;Serine/threonine-protein kinase;Transferase MISCELLANEOUS: Present with 7500 molecules/cell in log phase SD medium." P22525,"PROTEIN NAMES: Probable L,D-transpeptidase YcbB PROTEIN FAMILY: YkuD family ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the YkuD family. FUNCTION: Responsible, at least in part, for generating a meso-diaminopimelyl-3-a meso-diaminopimelyl-3 cross-link. PATHWAY: Cell wall biogenesis; peptidoglycan biosynthesis. KEYWORDS: 3D-structure;Cell shape;Cell wall biogenesis/degradation;Glycosyltransferase;Hydrolase;Membrane;Peptidoglycan synthesis;Reference proteome;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Single-pass membrane protein " P22695,"PROTEIN NAMES: Cytochrome b-c1 complex subunit 2, mitochondrial (Complex III subunit 2) (Core protein II) (Ubiquinol-cytochrome-c reductase complex core protein 2) PROTEIN FAMILY: Peptidase M16 family, UQCRC2/QCR2 subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the peptidase M16 family. UQCRC2/QCR2 subfamily. FUNCTION: Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. The cytochrome b-c1 complex catalyzes electron transfer from ubiquinol to cytochrome c, linking this redox reaction to translocation of protons across the mitochondrial inner membrane, with protons being carried across the membrane as hydrogens on the quinol. In the process called Q cycle, 2 protons are consumed from the matrix, 4 protons are released into the intermembrane space and 2 electrons are passed to cytochrome c (By similarity). The 2 core subunits UQCRC1/QCR1 and UQCRC2/QCR2 are homologous to the 2 mitochondrial-processing peptidase (MPP) subunits beta-MPP and alpha-MPP respectively, and they seem to have preserved their MPP processing properties (By similarity). May be involved in the in situ processing of UQCRFS1 into the mature Rieske protein and its mitochondrial targeting sequence (MTS)/subunit 9 when incorporated into complex III (Probable). KEYWORDS: 3D-structure;Acetylation;Direct protein sequencing;Disease variant;Electron transport;Membrane;Mitochondrion;Mitochondrion inner membrane;Primary mitochondrial disease;Reference proteome;Respiratory chain;Transit peptide;Transport SUBCELLULAR LOCATION: Mitochondrion inner membrane ; Peripheral membrane protein ; Matrix side " P22756,"PROTEIN NAMES: Glutamate receptor ionotropic, kainate 1 (GluK1) (Glutamate receptor 5) (GluR-5) (GluR5) PROTEIN FAMILY: Glutamate-gated ion channel family, GRIK1 subfamily ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIK1 subfamily. FUNCTION: Ionotropic glutamate receptor that functions as a cation-permeable ligand-gated ion channel, gated by L-glutamate and the glutamatergic agonist kainic acid. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. KEYWORDS: 3D-structure;Alternative splicing;Cell membrane;Disulfide bond;Glycoprotein;Ion channel;Ion transport;Ligand-gated ion channel;Membrane;Phosphoprotein;Postsynaptic cell membrane;Receptor;Reference proteome;RNA editing;Signal;Synapse;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Postsynaptic cell membrane ; Multi-pass membrane protein MISCELLANEOUS: The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. This receptor binds domoate > kainate > L-glutamate = quisqualate > CNQX = DNQX > AMPA > dihydrokainate > NMDA (By similarity)." P23179,"PROTEIN NAMES: Meiosis-specific protein SPO11 (Sporulation-specific protein 11) PROTEIN FAMILY: TOP6A family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the TOP6A family. FUNCTION: Required for meiotic recombination. Mediates DNA cleavage that forms the double-strand breaks (DSB) that initiate meiotic recombination. The action of SPO11 is important in setting off a regulatory chain of events encompassing 5' to 3' resection. When there are no SPO11-DSBs, resection of a site specific VDE-DSB takes place but it is faster than in wild-type meiosis and increases the risk of uncovering flanking homology. KEYWORDS: Chromosome;Direct protein sequencing;DNA-binding;Isomerase;Magnesium;Meiosis;Metal-binding;Nucleus;Reference proteome;Sporulation;Topoisomerase SUBCELLULAR LOCATION: Nucleus Chromosome " P23256,"PROTEIN NAMES: Protein MalY [Includes: Cystathionine beta-lyase MalY (CBL) (Beta-cystathionase MalY) (Cysteine lyase MalY) (Cysteine-S-conjugate beta-lyase MalY); Maltose regulon modulator] PROTEIN FAMILY: Class-II pyridoxal-phosphate-dependent aminotransferase family, MalY/PatB cystathionine beta-lyase subfamily ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. MalY/PatB cystathionine beta-lyase subfamily. FUNCTION: Acts as a beta-cystathionase and as a repressor of the maltose regulon. KEYWORDS: 3D-structure;Amino-acid biosynthesis;Lyase;Methionine biosynthesis;Pyridoxal phosphate;Reference proteome;Repressor;Transcription;Transcription regulation " P23472,"PROTEIN NAMES: Hevamine-A [Includes: Chitinase ; Lysozyme ] PROTEIN FAMILY: Glycosyl hydrolase 18 family, Chitinase class II subfamily ORGANISM: Hevea brasiliensis (Para rubber tree) (Siphonia brasiliensis) SIMILARITY: Belongs to the glycosyl hydrolase 18 family. Chitinase class II subfamily. FUNCTION: Bifunctional enzyme with lysozyme / chitinase activity. May have a role in plugging the latex vessel and cessation of latex flow. KEYWORDS: 3D-structure;Carbohydrate metabolism;Chitin degradation;Direct protein sequencing;Disulfide bond;Glycosidase;Hydrolase;Multifunctional enzyme;Polysaccharide degradation;Signal;Vacuole SUBCELLULAR LOCATION: Vacuole. Note=In the lutoids (vacuoles) from rubber latex. MISCELLANEOUS: Two components of hevamine have been isolated: hevamine A (shown here), the most abundant, and hevamine B." P23497,"PROTEIN NAMES: Nuclear autoantigen Sp-100 (Nuclear dot-associated Sp100 protein) (Speckled 100 kDa) ORGANISM: Homo sapiens (Human) FUNCTION: Together with PML, this tumor suppressor is a major constituent of the PML bodies, a subnuclear organelle involved in a large number of physiological processes including cell growth, differentiation and apoptosis. Functions as a transcriptional coactivator of ETS1 and ETS2 according to previous research. Under certain conditions, it may also act as a corepressor of ETS1 preventing its binding to DNA according to previous research. Through the regulation of ETS1 it may play a role in angiogenesis, controlling endothelial cell motility and invasion. Through interaction with the MRN complex it may be involved in the regulation of telomeres lengthening. May also regulate TP53-mediated transcription and through CASP8AP2, regulate FAS-mediated apoptosis. Also plays a role in infection by viruses, including human cytomegalovirus and Epstein-Barr virus, through mechanisms that may involve chromatin and/or transcriptional regulation. KEYWORDS: 3D-structure;Acetylation;Alternative splicing;Coiled coil;Cytoplasm;DNA-binding;Host-virus interaction;Isopeptide bond;Nucleus;Phosphoprotein;Reference proteome;Repeat;Transcription;Transcription regulation;Ubl conjugation SUBCELLULAR LOCATION: Nucleus Nucleus, PML body Nucleus, nuclear body Cytoplasm Note=Differences in the subnuclear localization of the different isoforms seem to exist and may also be cell cycle- and interferon-dependent. Accumulates in the cytoplasm upon FAS activation.; SUBCELLULAR LOCATION: [Isoform Sp100-C]: Nucleus Note=Forms a reticulate or track-like nuclear pattern with denser concentrations at the nuclear lamina and surrounding the nucleoli, a pattern reminiscent of heterochromatin-rich regions according to previous research. MISCELLANEOUS: The major isoform Sp100-A, has a calculated molecular weight of 54 kDa, but exhibits aberrant electrophoretic mobilities, with an apparent molecular weight of 100 kDa.; MISCELLANEOUS: [Isoform Sp100-A]: Major isoform." P23515,"PROTEIN NAMES: Oligodendrocyte-myelin glycoprotein ORGANISM: Homo sapiens (Human) FUNCTION: Cell adhesion molecule contributing to the interactive process required for myelination in the central nervous system. KEYWORDS: Cell adhesion;Cell membrane;Direct protein sequencing;Glycoprotein;GPI-anchor;Leucine-rich repeat;Lipoprotein;Membrane;Reference proteome;Repeat;Signal SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor, GPI-anchor. " P23640,"PROTEIN NAMES: Ras-related protein Rab-27A (Rab-27) (GTP-binding protein Ram) (Ram p25) PROTEIN FAMILY: Small GTPase superfamily, Rab family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the small GTPase superfamily. Rab family. FUNCTION: Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate homeostasis of late endocytic pathway, including endosomal positioning, maturation and secretion. Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse. KEYWORDS: Acetylation;Disulfide bond;Endosome;Exocytosis;GTP-binding;Hydrolase;Lipoprotein;Lysosome;Membrane;Methylation;Nucleotide-binding;Phosphoprotein;Prenylation;Reference proteome SUBCELLULAR LOCATION: Membrane ; Lipid-anchor Melanosome Late endosome Lysosome Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Localizes to endosomal exocytic vesicles. " P23654,PROTEIN NAMES: Neurotactin PROTEIN FAMILY: Type-B carboxylesterase/lipase family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: In the C-terminal section; belongs to the type-B carboxylesterase/lipase family. FUNCTION: May mediate or modulate cell adhesion between embryonic cells during development. KEYWORDS: Cell adhesion;Disulfide bond;Glycoprotein;Membrane;Phosphoprotein;Reference proteome;Signal-anchor;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Single-pass type II membrane protein Note=Expressed in membranes during embryogenesis. P23724,"PROTEIN NAMES: Proteasome subunit beta type-6 (Multicatalytic endopeptidase complex subunit C5) (Proteasome component C5) PROTEIN FAMILY: Peptidase T1B family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the peptidase T1B family. FUNCTION: Non-catalytic component of the proteasome which degrades poly-ubiquitinated proteins in the cytoplasm and in the nucleus. It is essential for the regulated turnover of proteins and for the removal of misfolded proteins. The proteasome is a multicatalytic proteinase complex that is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. It has an ATP-dependent proteolytic activity. KEYWORDS: 3D-structure;Cytoplasm;Nucleus;Proteasome;Reference proteome SUBCELLULAR LOCATION: Cytoplasm. Nucleus. " P23747,PROTEIN NAMES: Alginate biosynthesis transcriptional regulatory protein AlgB ORGANISM: Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) FUNCTION: Member of the two-component regulatory system AlgB/KinB involved in regulation of alginate biosynthesis genes. Positive regulator of the alginate biosynthetic gene AlgD. PATHWAY: Glycan biosynthesis; alginate biosynthesis [regulation]. KEYWORDS: Activator;Alginate biosynthesis;ATP-binding;DNA-binding;Nucleotide-binding;Phosphoprotein;Reference proteome;Transcription;Transcription regulation;Two-component regulatory system MISCELLANEOUS: In vivo phosphorylation of AlgB is not required for its role in alginate production. The mechanism by which it activates AlgD appears not to be mediated by conventional phosphorylation-dependent signal transduction. P23803,"PROTEIN NAMES: Protein odd-skipped PROTEIN FAMILY: Odd C2H2-type zinc-finger protein family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the Odd C2H2-type zinc-finger protein family. FUNCTION: Pair-rule protein that determines both the size and polarity of even-numbered as well as odd-numbered parasegments during embryogenesis. DNA-binding transcription factor that acts primarily as a transcriptional repressor but can also function as a transcriptional activator, depending on the stage of development and spatial restrictions. May function redundantly with odd and drm in leg joint formation during the larval stages, acting downstream of Notch activation. KEYWORDS: Activator;Developmental protein;DNA-binding;Metal-binding;Nucleus;Pair-rule protein;Reference proteome;Repeat;Repressor;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus " P23890,"PROTEIN NAMES: Transcriptional activator CadC (Membrane-integrated pH sensor CadC) ORGANISM: Escherichia coli (strain K12) FUNCTION: Regulates the lysine- and pH-dependent expression of the lysine decarboxylase CadA and the cadaverine-lysine antiporter CadB. At low external pH, and in the presence of external lysine, CadC activates transcription of the cadBA operon by binding directly to two sites, Cad1 and Cad2, within the cadBA promoter region (Pcad). Preferentially binds to AT-rich regions within the Cad1 promoter. KEYWORDS: 3D-structure;Activator;Cell inner membrane;Cell membrane;Disulfide bond;DNA-binding;Membrane;Reference proteome;Transcription;Transcription regulation;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell inner membrane ; Single-pass membrane protein Note=Membrane insertion requires the SecA translocase. " P23900,PROTEIN NAMES: Glycerol uptake/efflux facilitator protein PROTEIN FAMILY: MIP/aquaporin family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the MIP/aquaporin (TC 1.A.8) family. FUNCTION: Channel protein for glycerol. Has a role in both glycerol influx and efflux. Plays a role in osmoregulation: under osmotic stress the channel is apparently closed to allow accumulation of glycerol in the cell under hyperosmotic conditions. KEYWORDS: Glycerol metabolism;Membrane;Phosphoprotein;Reference proteome;Repeat;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Membrane ; Multi-pass membrane protein MISCELLANEOUS: Present with 907 molecules/cell in log phase SD medium. P24001,PROTEIN NAMES: Interleukin-32 (IL-32) (Natural killer cells protein 4) (Tumor necrosis factor alpha-inducing factor) ORGANISM: Homo sapiens (Human) FUNCTION: Cytokine that may play a role in innate and adaptive immune responses. It induces various cytokines such as TNFA/TNF-alpha and IL8. It activates typical cytokine signal pathways of NF-kappa-B and p38 MAPK. KEYWORDS: Alternative splicing;Cytokine;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted P24005,PROTEIN NAMES: Calcium-regulated actin-bundling protein (34 kDa actin-binding protein) ORGANISM: Dictyostelium discoideum (Social amoeba) FUNCTION: May contribute to the structure and reorganization of filopodia and pseudopodia accompanying cell movements. KEYWORDS: 3D-structure;Actin-binding;Calcium;Direct protein sequencing;Reference proteome P24207,"PROTEIN NAMES: Phenylalanine-specific permease (Phenylalanine:H(+) symporter PheP) PROTEIN FAMILY: Amino acid-polyamine-organocation (APC) superfamily, Amino acid transporter (AAT) family ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the amino acid-polyamine-organocation (APC) superfamily. Amino acid transporter (AAT) (TC 2.A.3.1) family. FUNCTION: Permease that is involved in the active transport across the cytoplasmic membrane of phenylalanine. Can also transport tyrosine, but not tryptophan. KEYWORDS: Amino-acid transport;Cell inner membrane;Cell membrane;Membrane;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell inner membrane ; Multi-pass membrane protein " P24309,"PROTEIN NAMES: Lariat debranching enzyme PROTEIN FAMILY: Lariat debranching enzyme family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the lariat debranching enzyme family. FUNCTION: Cleaves the 2'-5' phosphodiester linkage at the branch point of lariat intron pre-mRNAs after splicing and converts them into linear molecules that are subsequently degraded, thereby facilitating ribonucleotide turnover. It also participates in Ty1 retrovirus-like transposition via an RNA lariat intermediate in cDNA synthesis. KEYWORDS: Cytoplasm;Hydrolase;Iron;Manganese;Metal-binding;mRNA processing;Nucleus;Phosphoprotein;Reference proteome;RNA-binding;Zinc SUBCELLULAR LOCATION: Nucleus Cytoplasm MISCELLANEOUS: Present with 1130 molecules/cell in log phase SD medium." P24327,"PROTEIN NAMES: Foldase protein PrsA PROTEIN FAMILY: PrsA family ORGANISM: Bacillus subtilis (strain 168) SIMILARITY: Belongs to the PrsA family. FUNCTION: Essential protein that plays a major role in protein secretion by helping the post-translocational extracellular folding of several secreted proteins. Has PPIase activity but it is not essential for its function in vivo. KEYWORDS: 3D-structure;Cell membrane;Isomerase;Lipoprotein;Membrane;Palmitate;Reference proteome;Rotamase;Signal SUBCELLULAR LOCATION: Cell membrane ; Lipid-anchor Membrane raft ; Lipid-anchor Note=Present in detergent-resistant membrane (DRM) fractions that may be equivalent to eukaryotic membrane rafts; these rafts include proteins involved in signaling, molecule trafficking and protein secretion. " P24350,"PROTEIN NAMES: Inhibitory POU protein (I-POU) (Abnormal chemosensory jump 6 protein) PROTEIN FAMILY: POU transcription factor family, Class-4 subfamily ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the POU transcription factor family. Class-4 subfamily. FUNCTION: Modulates gene transcription; simultaneously generates both a specific activator and an inhibitor of gene transcription, capable of modulating two distinct regulatory programs during neural development. Has a role in olfactory behavior. KEYWORDS: Activator;Alternative splicing;DNA-binding;Homeobox;Nucleus;Olfaction;Reference proteome;Repressor;Sensory transduction;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus. " P24392,"PROTEIN NAMES: Peroxisome biogenesis factor 2 (Peroxin-2) (Peroxisomal membrane protein 3) (Peroxisome assembly factor 1) (PAF-1) PROTEIN FAMILY: Pex2/pex10/pex12 family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the pex2/pex10/pex12 family. FUNCTION: E3 ubiquitin-protein ligase component of a retrotranslocation channel required for peroxisome organization by mediating export of the PEX5 receptor from peroxisomes to the cytosol, thereby promoting PEX5 recycling. The retrotranslocation channel is composed of PEX2, PEX10 and PEX12; each subunit contributing transmembrane segments that coassemble into an open channel that specifically allows the passage of PEX5 through the peroxisomal membrane. PEX2 also regulates peroxisome organization by acting as a E3 ubiquitin-protein ligase. PEX2 ubiquitinates PEX5 during its passage through the retrotranslocation channel: catalyzes monoubiquitination of PEX5 at 'Cys-11', a modification that acts as a signal for PEX5 extraction into the cytosol (By similarity). Required for pexophagy in response to starvation by mediating ubiquitination of peroxisomal proteins, such as PEX5 and ABCD3/PMP70. Also involved in the response to reactive oxygen species (ROS) by mediating 'Lys-48'-linked polyubiquitination and subsequent degradation of PNPLA2/ATGL, thereby regulating lipolysis (By similarity). PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Disulfide bond;Membrane;Metal-binding;Peroxisome;Peroxisome biogenesis;Protein transport;Reference proteome;Transferase;Transmembrane;Transmembrane helix;Transport;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Peroxisome membrane ; Multi-pass membrane protein " P24592,PROTEIN NAMES: Insulin-like growth factor-binding protein 6 (IBP-6) (IGF-binding protein 6) (IGFBP-6) ORGANISM: Homo sapiens (Human) FUNCTION: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Activates the MAPK signaling pathway and induces cell migration. KEYWORDS: 3D-structure;Direct protein sequencing;Disulfide bond;Glycoprotein;Growth factor binding;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted P24863,"PROTEIN NAMES: Cyclin-C (SRB11 homolog) (hSRB11) PROTEIN FAMILY: Cyclin family, Cyclin C subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the cyclin family. Cyclin C subfamily. FUNCTION: Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Binds to and activates cyclin-dependent kinase CDK8 that phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. KEYWORDS: 3D-structure;Activator;Alternative splicing;Cyclin;Nucleus;Phosphoprotein;Reference proteome;Repressor;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " P24867,"PROTEIN NAMES: PHO85 cyclin-1 (Cyclin HCS26) (G1/S-specific cyclin PCL1) PROTEIN FAMILY: Cyclin family, PCL1,2 subfamily ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the cyclin family. PCL1,2 subfamily. FUNCTION: G1/S-specific cyclin partner of the cyclin-dependent kinase (CDK) PHO85. Essential for the control of the cell cycle at the G1/S (start) transition. The PCL1-PHO85 cyclin-CDK holoenzyme is involved in phosphorylation of the CDK inhibitor (CKI) SIC1, which is required for its ubiquitination and degradation, releasing repression of b-type cyclins and promoting exit from mitosis. Together with cyclin PCL2, positively controls degradation of sphingoid long chain base kinase LCB4. PCL1-PHO85 phosphorylates LCB4, which is required for its ubiquitination and degradation. PCL1-PHO85 also phosphorylates HMS1, NCP1 and NPA3, which may all have a role in mitotic exit. KEYWORDS: Cell cycle;Cell division;Cyclin;Cytoplasm;Isopeptide bond;Nucleus;Phosphoprotein;Reference proteome;Ubl conjugation SUBCELLULAR LOCATION: Cytoplasm Nucleus Note=Localizes to the incipient bud site. MISCELLANEOUS: Present with 606 molecules/cell in log phase SD medium." P24869,"PROTEIN NAMES: G2/mitotic-specific cyclin-2 PROTEIN FAMILY: Cyclin family, Cyclin AB subfamily ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the cyclin family. Cyclin AB subfamily. FUNCTION: Essential for the control of the cell cycle at the G2/M (mitosis) transition. Interacts with the CDC2 protein kinase to form MPF. G2/M cyclins accumulate steadily during G2 and are abruptly destroyed at mitosis. KEYWORDS: Acetylation;Cell cycle;Cell division;Cyclin;Mitosis;Reference proteome MISCELLANEOUS: Present with 339 molecules/cell in log phase SD medium." P25044,"PROTEIN NAMES: Tyrosine-protein phosphatase 1 (Protein-tyrosine phosphatase 1) (PTPase 1) PROTEIN FAMILY: Protein-tyrosine phosphatase family, Non-receptor class subfamily ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the protein-tyrosine phosphatase family. Non-receptor class subfamily. FUNCTION: Is not required for vegetative growth. KEYWORDS: Cytoplasm;Hydrolase;Phosphoprotein;Protein phosphatase;Reference proteome SUBCELLULAR LOCATION: Cytoplasm. MISCELLANEOUS: Present with 2690 molecules/cell in log phase SD medium." P25045,"PROTEIN NAMES: Serine palmitoyltransferase 1 (SPT 1) (SPT1) (Long chain base biosynthesis protein 1) PROTEIN FAMILY: Class-II pyridoxal-phosphate-dependent aminotransferase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. FUNCTION: Component of serine palmitoyltransferase (SPT), which catalyzes the committed step in the synthesis of sphingolipids, the condensation of serine with palmitoyl CoA to form the long chain base 3-ketosphinganine. PATHWAY: Lipid metabolism; sphingolipid metabolism. KEYWORDS: 3D-structure;Acyltransferase;Cytoplasm;Endoplasmic reticulum;Lipid metabolism;Membrane;Phosphoprotein;Pyridoxal phosphate;Reference proteome;Sphingolipid metabolism;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cytoplasm. Endoplasmic reticulum membrane; Multi-pass membrane protein. MISCELLANEOUS: Present with 22400 molecules/cell in log phase SD medium." P25052,"PROTEIN NAMES: Aminopyrimidine aminohydrolase (4-amino-5-aminomethyl-2-methylpyrimidine hydrolase) (Thiaminase II) PROTEIN FAMILY: TenA family ORGANISM: Bacillus subtilis (strain 168) SIMILARITY: Belongs to the TenA family. FUNCTION: Catalyzes an amino-pyrimidine hydrolysis reaction at the C5' of the pyrimidine moiety of thiamine compounds, a reaction that is part of a thiamine salvage pathway. Thus, catalyzes the conversion of 4-amino-5-aminomethyl-2-methylpyrimidine to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP). To a lesser extent, is also able to catalyze the hydrolytic cleavage of thiamine; however, this thiaminase activity is unlikely to be physiologically relevant. Therefore, is involved in the regeneration of the thiamine pyrimidine from thiamine degraded products present in the environment, rather than in thiamine degradation. PATHWAY: Cofactor biosynthesis; thiamine diphosphate biosynthesis. KEYWORDS: 3D-structure;Hydrolase;Reference proteome;Thiamine biosynthesis " P25119,PROTEIN NAMES: Tumor necrosis factor receptor superfamily member 1B (Tumor necrosis factor receptor 2) (TNF-R2) (Tumor necrosis factor receptor type II) (TNF-RII) (TNFR-II) (p75) (p80 TNF-alpha receptor) (CD antigen CD120b) ORGANISM: Mus musculus (Mouse) FUNCTION: Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2 (By similarity). KEYWORDS: Disulfide bond;Glycoprotein;Membrane;Phosphoprotein;Receptor;Reference proteome;Repeat;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. P25152,"PROTEIN NAMES: Aminopeptidase YwaD (Arginyl aminopeptidase) (BSAP) (Leucyl aminopeptidase) PROTEIN FAMILY: Peptidase M28 family, M28B subfamily ORGANISM: Bacillus subtilis (strain 168) SIMILARITY: Belongs to the peptidase M28 family. M28B subfamily. FUNCTION: Catalyzes the hydrolysis of a range of N-terminal amino acids. KEYWORDS: 3D-structure;Aminopeptidase;Hydrolase;Metal-binding;Metalloprotease;Protease;Reference proteome;Secreted;Signal;Zinc SUBCELLULAR LOCATION: Secreted " P25294,PROTEIN NAMES: Protein SIS1 ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Required for nuclear migration during mitosis. It is required for the normal initiation of translation. Might mediate the dissociation of a specific protein complex of the translation machinery. Essential for viability. KEYWORDS: 3D-structure;Cell cycle;Chaperone;Cytoplasm;DNA-binding;Nucleus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Nucleus Note=Localized throughout the cell but is more concentrated at the nucleus. MISCELLANEOUS: Present with 20300 molecules/cell in log phase SD medium. P25298,"PROTEIN NAMES: mRNA 3'-end-processing protein RNA14 ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Component of the cleavage factor IA (CFIA) complex, which is involved in the endonucleolytic cleavage during polyadenylation-dependent pre-mRNA 3'-end formation and cooperates with the cleavage factor NAB4/CFIB and the cleavage and polyadenylation factor (CPF) complex. KEYWORDS: 3D-structure;Cytoplasm;mRNA processing;Nucleus;Reference proteome;Repeat SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=Nucleus and/or cytoplasm. MISCELLANEOUS: Present with 5350 molecules/cell in log phase SD medium." P25299,"PROTEIN NAMES: mRNA 3'-end-processing protein RNA15 ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: RNA-binding component of the cleavage factor IA (CFIA) complex, which is involved in the endonucleolytic cleavage during polyadenylation-dependent pre-mRNA 3'-end formation and cooperates with the cleavage factor NAB4/CFIB and the cleavage and polyadenylation factor (CPF) complex. Binds to A-rich RNA sequence elements. KEYWORDS: 3D-structure;mRNA processing;Nucleus;Reference proteome;RNA-binding SUBCELLULAR LOCATION: Nucleus. MISCELLANEOUS: Present with 6350 molecules/cell in log phase SD medium." P25303,PROTEIN NAMES: DnaJ-related protein SCJ1 (J protein SCJ1) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Regulates protein folding in the endoplasmic reticulum lumen. Probably acts as a J-protein for the Hsp70-type chaperone KAR2 by stimulating its ATP-dependent reaction cycle and initiating folding reactions. Also involved in the endoplasmic reticulum-associated degradation (ERAD) process. Cooperates with KAR2 and another J-protein JEM1 to facilitate the export of ERAD substrates to the cytoplasm by maintaining them in a translocation-competent state and preventing their aggregation in the endoplasmic reticulum lumen. KEYWORDS: Chaperone;Endoplasmic reticulum;Metal-binding;Protein transport;Reference proteome;Repeat;Signal;Transport;Zinc;Zinc-finger SUBCELLULAR LOCATION: Endoplasmic reticulum lumen MISCELLANEOUS: Present with 8260 molecules/cell in log phase SD medium. P25340,"PROTEIN NAMES: Delta(24(24(1)))-sterol reductase ERG4 (C-24(28) sterol reductase ERG4) (Ergosterol biosynthetic protein 4) (Sterol Delta(24(28))-reductase ERG4) PROTEIN FAMILY: ERG4/ERG24 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the ERG4/ERG24 family. FUNCTION: C-24(28) sterol reductase; part of the third module of ergosterol biosynthesis pathway that includes the late steps of the pathway. ERG4 Catalyzes the last step of ergosterol biosynthesis by converting ergosta-5,7,22,24(28)-tetraen-3beta-ol into ergosterol. The third module or late pathway involves the ergosterol synthesis itself through consecutive reactions that mainly occur in the endoplasmic reticulum (ER) membrane. Firstly, the squalene synthase ERG9 catalyzes the condensation of 2 farnesyl pyrophosphate moieties to form squalene, which is the precursor of all steroids. Squalene synthase is crucial for balancing the incorporation of farnesyl diphosphate (FPP) into sterol and nonsterol isoprene synthesis. Secondly, the squalene epoxidase ERG1 catalyzes the stereospecific oxidation of squalene to (S)-2,3-epoxysqualene, which is considered to be a rate-limiting enzyme in steroid biosynthesis. Then, the lanosterol synthase ERG7 catalyzes the cyclization of (S)-2,3 oxidosqualene to lanosterol, a reaction that forms the sterol core. In the next steps, lanosterol is transformed to zymosterol through a complex process involving various demethylation, reduction and desaturation reactions. The lanosterol 14-alpha-demethylase ERG11 (also known as CYP51) catalyzes C14-demethylation of lanosterol to produce 4,4'-dimethyl cholesta-8,14,24-triene-3-beta-ol, which is critical for ergosterol biosynthesis. The C-14 reductase ERG24 reduces the C14=C15 double bond of 4,4-dimethyl-cholesta-8,14,24-trienol to produce 4,4-dimethyl-cholesta-8,24-dienol. 4,4-dimethyl-cholesta-8,24-dienol is substrate of the C-4 demethylation complex ERG25-ERG26-ERG27 in which ERG25 catalyzes the three-step monooxygenation required for the demethylation of 4,4-dimethyl and 4alpha-methylsterols, ERG26 catalyzes the oxidative decarboxylation that results in a reduction of the 3-beta-hydroxy group at the C-3 carbon to an oxo group, and ERG27 is responsible for the reduction of the keto group on the C-3. ERG28 has a role as a scaffold to help anchor ERG25, ERG26 and ERG27 to the endoplasmic reticulum and ERG29 regulates the activity of the iron-containing C4-methylsterol oxidase ERG25. Then, the sterol 24-C-methyltransferase ERG6 catalyzes the methyl transfer from S-adenosyl-methionine to the C-24 of zymosterol to form fecosterol. The C-8 sterol isomerase ERG2 catalyzes the reaction which results in unsaturation at C-7 in the B ring of sterols and thus converts fecosterol to episterol. The sterol-C5-desaturase ERG3 then catalyzes the introduction of a C-5 double bond in the B ring to produce 5-dehydroepisterol. The C-22 sterol desaturase ERG5 further converts 5-dehydroepisterol into ergosta-5,7,22,24(28)-tetraen-3beta-ol by forming the C-22(23) double bond in the sterol side chain. Finally, ergosta-5,7,22,24(28)-tetraen-3beta-ol is substrate of the C-24(28) sterol reductase ERG4 to produce ergosterol. PATHWAY: Steroid metabolism; ergosterol biosynthesis; ergosterol from zymosterol: step 5/5. KEYWORDS: Endoplasmic reticulum;Lipid biosynthesis;Lipid metabolism;Membrane;NADP;Oxidoreductase;Reference proteome;Steroid biosynthesis;Steroid metabolism;Sterol biosynthesis;Sterol metabolism;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein MISCELLANEOUS: Present with 1640 molecules/cell in log phase SD medium." P25344,PROTEIN NAMES: Protein STE50 ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Involved in growth arrest during conjugation. May interact with the G protein alpha subunit. KEYWORDS: 3D-structure;Cell cycle;Growth arrest;Pheromone response;Phosphoprotein;Reference proteome MISCELLANEOUS: Present with 1670 molecules/cell in log phase SD medium. P25353,PROTEIN NAMES: Ectonucleotide pyrophosphatase/phosphodiesterase 1 (E-NPP 1) [Includes: Alkaline phosphodiesterase 1 ; Nucleotide pyrophosphatase (NPPase) (Nucleotide diphosphatase)] PROTEIN FAMILY: Nucleotide pyrophosphatase/phosphodiesterase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the nucleotide pyrophosphatase/phosphodiesterase family. FUNCTION: Mediates extracellular nucleotide derived phosphate hydrolysis along with NPP2 and PHO5. KEYWORDS: Glycoprotein;Hydrolase;Membrane;Multifunctional enzyme;Phosphoprotein;Reference proteome;Signal-anchor;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Single-pass type II membrane protein MISCELLANEOUS: Present with 3420 molecules/cell in log phase SD medium. P25368,"PROTEIN NAMES: Ribosomal RNA-processing protein 7 PROTEIN FAMILY: RRP7 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the RRP7 family. FUNCTION: Plays an important role in the synthesis of 18S rRNA but is not required for the 5.8S and 25S pathway. Is necessary for the cleavage at site A2. Is required for efficient association of RPS27 with the pre-ribosomal particle. KEYWORDS: 3D-structure;Nucleus;Reference proteome;Ribosome biogenesis;rRNA processing SUBCELLULAR LOCATION: Nucleus, nucleolus MISCELLANEOUS: Present with 2610 molecules/cell in log phase SD medium." P25441,"PROTEIN NAMES: DNA-directed RNA polymerase III subunit RPC4 (RNA polymerase III subunit C4) (C53) (DNA-directed RNA polymerase III 47 kDa polypeptide) PROTEIN FAMILY: Eukaryotic RPC4/POLR3D RNA polymerase subunit family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the eukaryotic RPC4/POLR3D RNA polymerase subunit family. FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Essential for tRNA synthesis. The RPC53/RPC4-RPC37/RPC5 subcomplex is required for terminator recognition and reinitiation. KEYWORDS: 3D-structure;DNA-directed RNA polymerase;Nucleus;Phosphoprotein;Reference proteome;Transcription SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: Present with 998 molecules/cell in log phase SD medium." P25445,"PROTEIN NAMES: Tumor necrosis factor receptor superfamily member 6 (Apo-1 antigen) (Apoptosis-mediating surface antigen FAS) (FASLG receptor) (CD antigen CD95) ORGANISM: Homo sapiens (Human) FUNCTION: Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase CASP8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs CASP8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro). KEYWORDS: 3D-structure;Alternative splicing;Apoptosis;Calmodulin-binding;Cell membrane;Direct protein sequencing;Disease variant;Disulfide bond;Glycoprotein;Lipoprotein;Membrane;Palmitate;Phosphoprotein;Receptor;Reference proteome;Repeat;Secreted;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: [Isoform 1]: Cell membrane ; Single-pass type I membrane protein Membrane raft.; SUBCELLULAR LOCATION: [Isoform 2]: Secreted.; SUBCELLULAR LOCATION: [Isoform 3]: Secreted.; SUBCELLULAR LOCATION: [Isoform 4]: Secreted.; SUBCELLULAR LOCATION: [Isoform 5]: Secreted.; SUBCELLULAR LOCATION: [Isoform 6]: Secreted. MISCELLANEOUS: [Isoform 2]: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.; MISCELLANEOUS: [Isoform 3]: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.; MISCELLANEOUS: [Isoform 4]: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.; MISCELLANEOUS: [Isoform 5]: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.; MISCELLANEOUS: [Isoform 7]: Dominant negative isoform, resistant to Fas-mediated apoptosis." P25490,"PROTEIN NAMES: Transcriptional repressor protein YY1 (Delta transcription factor) (INO80 complex subunit S) (NF-E1) (Yin and yang 1) (YY-1) PROTEIN FAMILY: YY transcription factor family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the YY transcription factor family. FUNCTION: Multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. Binds to the consensus sequence 5'-CCGCCATNTT-3'; some genes have been shown to contain a longer binding motif allowing enhanced binding; the initial CG dinucleotide can be methylated greatly reducing the binding affinity. The effect on transcription regulation is depending upon the context in which it binds and diverse mechanisms of action include direct activation or repression, indirect activation or repression via cofactor recruitment, or activation or repression by disruption of binding sites or conformational DNA changes. Its activity is regulated by transcription factors and cytoplasmic proteins that have been shown to abrogate or completely inhibit YY1-mediated activation or repression. For example, it acts as a repressor in absence of adenovirus E1A protein but as an activator in its presence. Acts synergistically with the SMAD1 and SMAD4 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression. Binds to SMAD binding elements (SBEs) (5'-GTCT/AGAC-3') within BMP response element (BMPRE) of cardiac activating regions. May play an important role in development and differentiation. Proposed to recruit the PRC2/EED-EZH2 complex to target genes that are transcriptional repressed. Involved in DNA repair. In vitro, binds to DNA recombination intermediate structures (Holliday junctions). Plays a role in regulating enhancer activation.; FUNCTION: Proposed core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair; proposed to target the INO80 complex to YY1-responsive elements. KEYWORDS: 3D-structure;Activator;ADP-ribosylation;Differentiation;Direct protein sequencing;Disease variant;DNA damage;DNA recombination;DNA repair;DNA-binding;Intellectual disability;Isopeptide bond;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Repeat;Repressor;Spermatogenesis;Transcription;Transcription regulation;Ubl conjugation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus matrix Note=Associated with the nuclear matrix. " P25491,"PROTEIN NAMES: Mitochondrial protein import protein MAS5 (Yeast dnaJ protein 1) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Probably involved in mitochondrial protein import. Is also required for efficient translocation of pre-pro-alpha-factor. Involved in heme regulation of HAP1, as a component of the high-molecular-weight (HMC) complex. KEYWORDS: 3D-structure;Chaperone;Cytoplasm;Isopeptide bond;Lipoprotein;Metal-binding;Methylation;Prenylation;Protein transport;Reference proteome;Repeat;Stress response;Transport;Ubl conjugation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cytoplasm. Cytoplasm, perinuclear region. Note=Concentrated in a perinuclear ring as well as in the cytoplasm. MISCELLANEOUS: Present with 119000 molecules/cell in log phase SD medium." P25524,"PROTEIN NAMES: Cytosine deaminase (CD) (CDA) (CDase) (Cytosine aminohydrolase) (Isoguanine deaminase) PROTEIN FAMILY: Metallo-dependent hydrolases superfamily, Cytosine deaminase family ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the metallo-dependent hydrolases superfamily. Cytosine deaminase family. FUNCTION: Catalyzes the hydrolytic deamination of cytosine to uracil. Is involved in the pyrimidine salvage pathway, which allows the cell to utilize cytosine for pyrimidine nucleotide synthesis. Is also able to catalyze deamination of isoguanine, a mutagenic oxidation product of adenine in DNA, and of isocytosine. To a lesser extent, also catalyzes the conversion of 5-fluorocytosine (5FC) to 5-fluorouracil (5FU); this activity allows the formation of a cytotoxic chemotherapeutic agent from a non-cytotoxic precursor. KEYWORDS: 3D-structure;Cytosine metabolism;Direct protein sequencing;Hydrolase;Iron;Metal-binding;Reference proteome;Zinc " P25573,"PROTEIN NAMES: Mitochondrial inner membrane i-AAA protease supercomplex subunit MGR1 (Mitochondrial genome-required protein 1) PROTEIN FAMILY: MGR1 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the MGR1 family. FUNCTION: Component of the mitochondrial inner membrane i-AAA protease supercomplex required for mitochondrial inner membrane protein turnover. Together with MGR3, functions in an adapter complex that targets substrates to the i-AAA protease for degradation. Required for growth of cells lacking the mitochondrial genome. KEYWORDS: Membrane;Mitochondrion;Mitochondrion inner membrane;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Mitochondrion inner membrane ; Multi-pass membrane protein MISCELLANEOUS: Present with 3410 molecules/cell in log phase SD medium." P25591,"PROTEIN NAMES: vacuole-related protein 17 (Vacuole-specific MYO2 receptor VAC17) PROTEIN FAMILY: VAC17 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the VAC17 family. FUNCTION: Vacuole-specific MYO2 receptor required for vacuole inheritance. Binds simultaneously to MYO2 and to VAC8, a vacuolar membrane protein, forming a transport complex which moves the attached vacuole membrane along actin cables into the bud. Once the vacuole arrives in the bud, VAC17 is degraded, depositing the vacuole in its correct location. KEYWORDS: 3D-structure;Acetylation;Membrane;Phosphoprotein;Reference proteome;Vacuole SUBCELLULAR LOCATION: Vacuole membrane ; Peripheral membrane protein ; Cytoplasmic side " P25604,"PROTEIN NAMES: Suppressor protein STP22 of temperature-sensitive alpha-factor receptor and arginine permease (ESCRT-I complex subunit VPS23) (Vacuolar protein sorting-associated protein 23) PROTEIN FAMILY: Ubiquitin-conjugating enzyme family, UEV subfamily ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the ubiquitin-conjugating enzyme family. UEV subfamily. FUNCTION: Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association to the ESCRT-0 complex. Required for vacuolar targeting of temperature-sensitive plasma membrane proteins STE2 and CAN1. KEYWORDS: 3D-structure;Coiled coil;Cytoplasm;Endosome;Membrane;Protein transport;Reference proteome;Transport SUBCELLULAR LOCATION: Cytoplasm. Endosome. Late endosome membrane ; Peripheral membrane protein MISCELLANEOUS: Present with 1360 molecules/cell in log phase SD medium." P25637,"PROTEIN NAMES: Protein IMPACT homolog PROTEIN FAMILY: IMPACT family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the IMPACT family. FUNCTION: Translational regulator that ensures constant high levels of translation under amino acid starvation. Plays a role as a negative regulator of the GCN2 kinase activity; impairs GCN1-mediated GCN2 activation, and hence GCN2-mediated eIF-2-alpha phosphorylation in amino acid-starved cells and subsequent down-regulation of protein synthesis. In normal conditions, it resides in a actin complex and has no activity. KEYWORDS: 3D-structure;Actin-binding;Cytoplasm;Isopeptide bond;Nucleus;Reference proteome;Repressor;Stress response;Translation regulation;Ubl conjugation SUBCELLULAR LOCATION: Cytoplasm Nucleus MISCELLANEOUS: Present with 3030 molecules/cell in log phase SD medium." P25644,"PROTEIN NAMES: Deadenylation-dependent mRNA-decapping factor PAT1 (DNA topoisomerase 2-associated protein PAT1) (Decapping activator and translational repressor PAT1) (Topoisomerase II-associated protein PAT1) (mRNA turnover protein 1) PROTEIN FAMILY: PAT1 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the PAT1 family. FUNCTION: Activator of decapping that functions as a general and active mechanism of translational repression and required for P-body formation. First decay factor recruited to mRNA, at a time when the mRNA is still associated with translation factors. Subsequently, PAT1 recruits the hepta-heterodimer LSM1-LSM7 complex to P-bodies. In association with the LSM1-LSM7 complex, stabilizes the 3' terminus of mRNAs. This association is also required for mosaic virus genomic RNA translation. Modulates the rates of mRNA-decapping that occur following deadenylation. Might be required for promoting the formation or the stabilization of the preinitiation translation complexes. Required for 40S ribosomal subunit joining to capped and/or polyadenylated mRNA. With other P-body components, enhances the formation of retrotransposition-competent Ty1 virus-like particles. Structural component of the kinetochore and associates with centromeres in a NDC10-dependent manner. Involved in maintaining the structural integrity of centromeric chromatin to facilitate faithful chromosome segregation and proper kinetochore function. KEYWORDS: 3D-structure;Acetylation;Cell cycle;Cell division;Centromere;Chromosome;Cytoplasm;Kinetochore;mRNA processing;Nucleus;Phosphoprotein;Reference proteome;Repressor;RNA-binding;Translation regulation SUBCELLULAR LOCATION: Cytoplasm Nucleus Cytoplasm, P-body Chromosome, centromere, kinetochore Cytoplasm, Stress granule Note=Shuttles between cytoplasm and nucleus. Exported in complex with LSM1. MISCELLANEOUS: Present with 656 molecules/cell in log phase SD medium." P25686,"PROTEIN NAMES: DnaJ homolog subfamily B member 2 (Heat shock 40 kDa protein 3) (Heat shock protein J1) (HSJ-1) ORGANISM: Homo sapiens (Human) FUNCTION: Functions as a co-chaperone, regulating the substrate binding and activating the ATPase activity of chaperones of the HSP70/heat shock protein 70 family. In parallel, also contributes to the ubiquitin-dependent proteasomal degradation of misfolded proteins. Thereby, may regulate the aggregation and promote the functional recovery of misfolded proteins like HTT, MC4R, PRKN, RHO and SOD1 and be crucial for many biological processes. Isoform 1 which is localized to the endoplasmic reticulum membranes may specifically function in ER-associated protein degradation of misfolded proteins. KEYWORDS: 3D-structure;Acetylation;Alternative splicing;Chaperone;Cytoplasm;Endoplasmic reticulum;Lipoprotein;Membrane;Methylation;Neurodegeneration;Neuropathy;Nucleus;Phosphoprotein;Prenylation;Reference proteome;Repeat;Ubl conjugation SUBCELLULAR LOCATION: [Isoform 2]: Cytoplasm Nucleus.; SUBCELLULAR LOCATION: [Isoform 1]: Endoplasmic reticulum membrane ; Lipid-anchor ; Cytoplasmic side " P26151,"PROTEIN NAMES: High affinity immunoglobulin gamma Fc receptor I (IgG Fc receptor I) (Fc-gamma RI) (FcRI) (CD antigen CD64) PROTEIN FAMILY: Immunoglobulin superfamily, FCGR1 family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the immunoglobulin superfamily. FCGR1 family. FUNCTION: High affinity receptor for the Fc region of immunoglobulins gamma. Functions in both innate and adaptive immune responses. KEYWORDS: Cell membrane;Disulfide bond;Glycoprotein;IgG-binding protein;Immunity;Immunoglobulin domain;Innate immunity;Membrane;Phosphoprotein;Receptor;Reference proteome;Repeat;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. Note=Stabilized at the cell membrane through interaction with FCER1G. " P26216,"PROTEIN NAMES: Exopolygalacturonase (ExoPG) (Galacturan 1,4-alpha-galacturonidase) (Pectinase) PROTEIN FAMILY: Glycosyl hydrolase 28 family ORGANISM: Zea mays (Maize) SIMILARITY: Belongs to the glycosyl hydrolase 28 family. FUNCTION: May function in depolymerizing pectin during pollen development, germination, and tube growth. Acts as an exo-polygalacturonase. KEYWORDS: Cell wall;Cell wall biogenesis/degradation;Direct protein sequencing;Disulfide bond;Glycoprotein;Glycosidase;Hydrolase;Reference proteome;Repeat;Secreted;Signal SUBCELLULAR LOCATION: Secreted. Secreted, cell wall. " P26342,"PROTEIN NAMES: Transforming growth factor beta receptor type 3 (TGF-beta receptor type 3) (TGFR-3) (Betaglycan) (Transforming growth factor beta receptor III) (TGF-beta receptor type III) ORGANISM: Rattus norvegicus (Rat) FUNCTION: Binds to TGF-beta. Could be involved in capturing and retaining TGF-beta for presentation to the signaling receptors. In gonadotrope cells, acts as an inhibin A coreceptor and regulates follicle-stimulating hormone (FSH) levels and female fertility (By similarity). KEYWORDS: 3D-structure;Cell membrane;Direct protein sequencing;Disulfide bond;Extracellular matrix;Glycoprotein;Membrane;Proteoglycan;Receptor;Reference proteome;Secreted;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein Secreted Secreted, extracellular space, extracellular matrix Note=Exists both as a membrane-bound form and as soluble form in serum and in the extracellular matrix. " P26359,"PROTEIN NAMES: DNA mismatch repair protein msh3 (Mating-type switching protein swi4) (MutS protein homolog 3) PROTEIN FAMILY: DNA mismatch repair MutS family, MSH3 subfamily ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the DNA mismatch repair MutS family. MSH3 subfamily. FUNCTION: Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with msh2 to form MutS beta, which binds to DNA mismatches thereby initiating DNA repair. Msh3 provides substrate-binding and substrate specificity to the complex. When bound, the MutS beta heterodimer bends the DNA helix and shields approximately 20 base pairs. Acts mainly to repair insertion-deletion loops (IDLs) from 2 to 13 nucleotides in size, but can also repair base-base and single insertion-deletion mismatches that occur during replication. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions (By similarity). Involved in termination of copy-synthesis during mating-type switching. KEYWORDS: ATP-binding;DNA damage;DNA repair;DNA-binding;Nucleotide-binding;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus " P26370,"PROTEIN NAMES: Transcriptional activator protein UGA3 ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: GABA-dependent positive regulation of genes required for catabolism of GABA (UGA4, UGA1, and UGA2). KEYWORDS: Activator;DNA-binding;Metal-binding;Nucleus;Reference proteome;Transcription;Transcription regulation;Zinc SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: Present with 1200 molecules/cell in log phase SD medium." P26449,PROTEIN NAMES: Cell cycle arrest protein BUB3 PROTEIN FAMILY: WD repeat BUB3 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the WD repeat BUB3 family. FUNCTION: Required for cell cycle arrest in response to loss of microtubule function. Component of the spindle assembly checkpoint which is a feedback control that prevents cells with incompletely assembled spindles from leaving mitosis. Component of the mitotic checkpoint complex (MCC) which inhibits the ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) by preventing its activation by CDC20. The formation of a MAD1-BUB1-BUB3 complex seems to be required for the spindle checkpoint mechanism. KEYWORDS: 3D-structure;Cell cycle;Nucleus;Phosphoprotein;Reference proteome;Repeat;WD repeat SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: Present with 1430 molecules/cell in log phase SD medium. P26842,PROTEIN NAMES: CD27 antigen (CD27L receptor) (T-cell activation antigen CD27) (T14) (Tumor necrosis factor receptor superfamily member 7) (CD antigen CD27) ORGANISM: Homo sapiens (Human) FUNCTION: Receptor specifically expressed at the surface of T cells which binds and is activated by its ligand CD70/CD27L expressed by B cells. The CD70-CD27 signaling pathway mediates antigen-specific T cell activation and expansion which in turn provides immune surveillance of B cells. KEYWORDS: 3D-structure;Apoptosis;Cell membrane;Direct protein sequencing;Disease variant;Disulfide bond;Glycoprotein;Membrane;Phosphoprotein;Receptor;Reference proteome;Repeat;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein P27091,PROTEIN NAMES: Protein 60A (Protein glass bottom boat) PROTEIN FAMILY: TGF-beta family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the TGF-beta family. FUNCTION: Required for the growth of imaginal tissues and for patterning of the adult wing. KEYWORDS: Cleavage on pair of basic residues;Cytokine;Developmental protein;Disulfide bond;Glycoprotein;Growth factor;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted P27128,"PROTEIN NAMES: Lipopolysaccharide glucosyltransferase WaaO (Lipopolysaccharide alpha-1,3 glucosyltransferase) (Lipopolysaccharide glucosyltransferase II) PROTEIN FAMILY: Glycosyltransferase 8 family ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the glycosyltransferase 8 family. FUNCTION: Glucosyltransferase involved in the biosynthesis of the core oligosaccharide region of lipopolysaccharide (LPS). Catalyzes the addition of a second glucose (glucose II) to the first outer-core glucose (glucose I). In vitro, can add multiple glucose residues to its lipid acceptor. Activity does not require the branched galactose added by WaaB, but it is higher in the presence of this branched galactose. In the absence of a lipid acceptor, can hydrolyze UDP-glucose, but not UDP-galactose. PATHWAY: Bacterial outer membrane biogenesis; LPS core biosynthesis. KEYWORDS: Glycosyltransferase;Lipopolysaccharide biosynthesis;Magnesium;Metal-binding;Reference proteome;Transferase " P27170,"PROTEIN NAMES: Serum paraoxonase/arylesterase 1 (PON 1) (Aromatic esterase 1) (A-esterase 1) (Serum aryldialkylphosphatase 1) PROTEIN FAMILY: Paraoxonase family ORGANISM: Oryctolagus cuniculus (Rabbit) SIMILARITY: Belongs to the paraoxonase family. FUNCTION: Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification. KEYWORDS: Antioxidant;Calcium;Direct protein sequencing;Disulfide bond;Glycoprotein;HDL;Hydrolase;Metal-binding;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted, extracellular space. MISCELLANEOUS: The preferential association of PON1 with HDL is mediated in part by its signal peptide, by binding phospholipids directly, rather than binding apo AI. The retained signal peptide may allow transfer of the protein between phospholipid surfaces." P27177,"PROTEIN NAMES: Major prion protein homolog (65-21 protein) (Acetylcholine receptor-inducing activity) (ARIA) (PR-LP) PROTEIN FAMILY: Prion family ORGANISM: Gallus gallus (Chicken) SIMILARITY: Belongs to the prion family. FUNCTION: Its primary physiological function is unclear. Has cytoprotective activity against internal or environmental stresses. May play a role in neuronal development and synaptic plasticity. May be required for neuronal myelin sheath maintenance. May play a role in iron uptake and iron homeostasis. Soluble oligomers are toxic to cultured neuroblastoma cells and induce apoptosis (in vitro). Association with GPC1 (via its heparan sulfate chains) targets PRNP to lipid rafts. Also provides Cu(2+) or Zn(2+) for the ascorbate-mediated GPC1 deaminase degradation of its heparan sulfate side chains (By similarity). KEYWORDS: 3D-structure;Amyloid;Cell membrane;Copper;Direct protein sequencing;Disulfide bond;Glycoprotein;GPI-anchor;Lipoprotein;Membrane;Metal-binding;Prion;Reference proteome;Repeat;Signal SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor, GPI-anchor. " P27250,"PROTEIN NAMES: Aldehyde reductase Ahr (Zinc-dependent alcohol dehydrogenase Ahr) PROTEIN FAMILY: Zinc-containing alcohol dehydrogenase family ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the zinc-containing alcohol dehydrogenase family. FUNCTION: Catalyzes the reduction of a wide range of aldehydes including aliphatic fatty aldehydes (C4-C16), into their corresponding alcohols. Has a strong preference for NADPH over NADH as the electron donor. Cannot use glyceraldehyde or a ketone as substrate. Is a relevant source of NADPH-dependent aldehyde reductase activity in E.coli. The in vivo functions of Ahr has yet to be determined. KEYWORDS: 3D-structure;Fatty acid metabolism;Lipid metabolism;Metal-binding;NADP;Oxidoreductase;Reference proteome;Zinc " P27296,"PROTEIN NAMES: ATP-dependent DNA helicase DinG PROTEIN FAMILY: Helicase family, DinG subfamily, Type 1 sub-subfamily ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the helicase family. DinG subfamily. Type 1 sub-subfamily. FUNCTION: DNA-dependent ATPase and 5'-3' DNA helicase. Can also unwind DNA-RNA hybrid duplexes. Is active on D-loops and R-loops, and on forked structures. May be involved in recombinational DNA repair and the resumption of replication after DNA damage. The redox cluster is involved in DNA-mediated charge-transport signaling between DNA repair proteins from distinct pathways. DinG cooperates at long-range with endonuclease III, a base excision repair enzyme, using DNA charge transport to redistribute to regions of DNA damage. KEYWORDS: 3D-structure;4Fe-4S;ATP-binding;DNA damage;DNA recombination;DNA repair;DNA-binding;Helicase;Hydrolase;Iron;Iron-sulfur;Metal-binding;Nucleotide-binding;Reference proteome " P27344,"PROTEIN NAMES: DNA polymerase epsilon subunit C (DNA polymerase II subunit C) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: As accessory component of the DNA polymerase epsilon (DNA polymerase II) participates in chromosomal DNA replication. It is required during synthesis of the leading and lagging DNA strands at the replication fork and binds at/or near replication origins and moves along DNA with the replication fork. It has 3'-5' proofreading exonuclease activity that correct errors arising during DNA replication. It is also involved in DNA synthesis during DNA repair. KEYWORDS: 3D-structure;DNA replication;Nucleus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: In eukaryotes there are five DNA polymerases: alpha, beta, gamma, delta, and epsilon which are responsible for different reactions of DNA synthesis.; MISCELLANEOUS: Present with 784 molecules/cell in log phase SD medium." P27443,"PROTEIN NAMES: NAD-dependent malic enzyme, mitochondrial (NAD-ME) PROTEIN FAMILY: Malic enzymes family ORGANISM: Ascaris suum (Pig roundworm) (Ascaris lumbricoides) SIMILARITY: Belongs to the malic enzymes family. KEYWORDS: 3D-structure;Allosteric enzyme;Metal-binding;Mitochondrion;NAD;Oxidoreductase;Transit peptide SUBCELLULAR LOCATION: Mitochondrion matrix. MISCELLANEOUS: This isoenzyme can also use NADP(+) but is more effective with NAD(+)." P27514,PROTEIN NAMES: Low-affinity phosphate transporter PHO91 PROTEIN FAMILY: CitM transporter family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the CitM (TC 2.A.11) transporter family. FUNCTION: Vacuolar phosphate transporter that probably exports phosphate from the vacuolar lumen to the cytosol. KEYWORDS: Membrane;Phosphate transport;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix;Transport;Ubl conjugation;Vacuole SUBCELLULAR LOCATION: Vacuole membrane ; Multi-pass membrane protein MISCELLANEOUS: Present with 4280 molecules/cell in log phase SD medium. P27544,"PROTEIN NAMES: Ceramide synthase 1 (CerS1) (LAG1 longevity assurance homolog 1) (Longevity assurance gene 1 protein homolog 1) (Protein UOG-1) (Sphingoid base N-stearoyltransferase CERS1) ORGANISM: Homo sapiens (Human) FUNCTION: Ceramide synthase that catalyzes the transfer of the acyl chain from acyl-CoA to a sphingoid base, with high selectivity toward stearoyl-CoA (octadecanoyl-CoA; C18:0-CoA). N-acylates sphinganine and sphingosine bases to form dihydroceramides and ceramides in de novo synthesis and salvage pathways, respectively. Plays a predominant role in skeletal muscle in regulating C18 ceramide and dihydroceramide levels with an impact on whole-body glucose metabolism and insulin sensitivity. Protects from diet-induced obesity by suppressing the uptake of glucose in multiple organs in a FGF21-dependent way (By similarity). Generates C18 ceramides in the brain, playing a critical role in cerebellar development and Purkinje cell function (By similarity). In response to cellular stress mediates mitophagy, a known defense mechanism against cell transformation and aging. Upon mitochondria fission, generates C18 ceramides that anchor lipidated MAP1LC3B/LC3B-II autophagolysosomes to outer mitochondrial membranes to eliminate damaged mitochondria. PATHWAY: Lipid metabolism; sphingolipid metabolism. KEYWORDS: Acetylation;Alternative splicing;Disease variant;Endoplasmic reticulum;Epilepsy;Lipid biosynthesis;Lipid metabolism;Membrane;Neurodegeneration;Reference proteome;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein MISCELLANEOUS: This protein is produced by a bicistronic gene which also produces the GDF1 protein from a non-overlapping reading frame." P27590,"PROTEIN NAMES: Uromodulin (Tamm-Horsfall urinary glycoprotein) (THP) [Cleaved into: Uromodulin, secreted form] ORGANISM: Rattus norvegicus (Rat) FUNCTION: [Uromodulin]: Functions in biogenesis and organization of the apical membrane of epithelial cells of the thick ascending limb of Henle's loop (TALH), where it promotes formation of complex filamentous gel-like structure that may play a role in the water barrier permeability. May serve as a receptor for binding and endocytosis of cytokines (IL-1, IL-2) and TNF. Facilitates neutrophil migration across renal epithelia.; FUNCTION: [Uromodulin, secreted form]: In the urine, may contribute to colloid osmotic pressure, retards passage of positively charged electrolytes, and inhibits formation of liquid containing supersaturated salts and subsequent formation of salt crystals. Protects against urinary tract infections by binding to type 1 fimbriated E.coli. Binds to bacterial adhesin fimH which mediates the stable formation of bacterial aggregates, prevents the binding of E.coli to uroplakins UPK1A and UPK1B which act as urothelial receptors for type I fimbriae, and allows for pathogen clearance through micturation. Also promotes aggregation of other bacteria including K.pneumoniae, P.aeruginosa and S.mitis and so may also protect against other uropathogens. KEYWORDS: Cell membrane;Cell projection;Disulfide bond;EGF-like domain;Glycoprotein;GPI-anchor;Immunity;Innate immunity;Lipoprotein;Membrane;Reference proteome;Repeat;Secreted;Signal SUBCELLULAR LOCATION: Apical cell membrane ; Lipid-anchor, GPI-anchor Basolateral cell membrane ; Lipid-anchor, GPI-anchor Cell projection, cilium membrane Note=Only a small fraction sorts to the basolateral pole of tubular epithelial cells compared to apical localization. Secreted into urine after cleavage. Colocalizes with NPHP1 and KIF3A.; SUBCELLULAR LOCATION: [Uromodulin, secreted form]: Secreted Note=Detected in urine. " P27619,"PROTEIN NAMES: Dynamin (Protein shibire) (dDyn) PROTEIN FAMILY: TRAFAC class dynamin-like GTPase superfamily, Dynamin/Fzo/YdjA family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family. FUNCTION: Microtubule-associated force-producing protein which is involved in the production of microtubule bundles and which is able to bind and hydrolyze GTP. Implicated in endocytic protein sorting. KEYWORDS: Alternative splicing;Cytoplasm;Cytoskeleton;Endocytosis;GTP-binding;Hydrolase;Microtubule;Motor protein;Nucleotide-binding;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cytoplasm. Cytoplasm, cytoskeleton. Note=Microtubule-associated. MISCELLANEOUS: Shibire mutation is the cause of temperature-sensitive paralysis. This is believed to be due to a reversible block of endocytosis, which prevents membrane cycling and thus depletes synaptic vesicles.; MISCELLANEOUS: 'Shibire' means 'paralyzed' in Japanese." P27867,"PROTEIN NAMES: Sorbitol dehydrogenase (SDH) (L-iditol 2-dehydrogenase) (Polyol dehydrogenase) (Xylitol dehydrogenase) (XDH) PROTEIN FAMILY: Zinc-containing alcohol dehydrogenase family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the zinc-containing alcohol dehydrogenase family. FUNCTION: Polyol dehydrogenase that catalyzes the reversible NAD(+)-dependent oxidation of various sugar alcohols (By similarity). Is active with D-sorbitol (D-glucitol) leading to the C2-oxidized product D-fructose. Is a key enzyme in the polyol pathway that interconverts glucose and fructose via sorbitol, which constitutes an important alternate route for glucose metabolism. May play a role in sperm motility by using sorbitol as an alternative energy source for sperm motility (By similarity). KEYWORDS: Acetylation;Cell projection;Cilium;Flagellum;Membrane;Metal-binding;Mitochondrion;NAD;Oxidoreductase;Phosphoprotein;Reference proteome;Zinc SUBCELLULAR LOCATION: Mitochondrion membrane ; Peripheral membrane protein Cell projection, cilium, flagellum Note=Associated with mitochondria of the midpiece and near the plasma membrane in the principal piece of the flagellum. Also found in the epididymosome, secreted by the epididymal epithelium and that transfers proteins from the epididymal fluid to the sperm surface. " P27922,"PROTEIN NAMES: Sodium-dependent dopamine transporter (DA transporter) (DAT) (Solute carrier family 6 member 3) PROTEIN FAMILY: Sodium:neurotransmitter symporter (SNF) family, SLC6A3 subfamily ORGANISM: Bos taurus (Bovine) SIMILARITY: Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A3 subfamily. FUNCTION: Mediates sodium- and chloride-dependent transport of dopamine. Also mediates sodium- and chloride-dependent transport of norepinephrine (also known as noradrenaline) (By similarity). Regulator of light-dependent retinal hyaloid vessel regression, downstream of OPN5 signaling (By similarity). KEYWORDS: Cell membrane;Cell projection;Disulfide bond;Glycoprotein;Membrane;Metal-binding;Neurotransmitter transport;Reference proteome;Sodium;Symport;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Cell projection, neuron projection Cell projection, axon Note=Localizes to neurite tips in neuronal cells (By similarity). Colocalizes with SEPTIN4 at axon terminals, especially at the varicosities (By similarity). MISCELLANEOUS: This protein is the target of psychomotor stimulants such as amphetamines or cocaine." P28067,"PROTEIN NAMES: HLA class II histocompatibility antigen, DM alpha chain (MHC class II antigen DMA) (Really interesting new gene 6 protein) PROTEIN FAMILY: MHC class II family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the MHC class II family. FUNCTION: Plays a critical role in catalyzing the release of class II-associated invariant chain peptide (CLIP) from newly synthesized MHC class II molecules and freeing the peptide binding site for acquisition of antigenic peptides. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. KEYWORDS: 3D-structure;Adaptive immunity;Disulfide bond;Endosome;Glycoprotein;Immunity;Lysosome;Membrane;MHC II;Reference proteome;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Late endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein. Note=Localizes to late endocytic compartment. Associates with lysosome membranes. " P28070,"PROTEIN NAMES: Proteasome subunit beta type-4 (26 kDa prosomal protein) (HsBPROS26) (PROS-26) (Macropain beta chain) (Multicatalytic endopeptidase complex beta chain) (Proteasome beta chain) (Proteasome chain 3) (HsN3) PROTEIN FAMILY: Peptidase T1B family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the peptidase T1B family. FUNCTION: Non-catalytic component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. KEYWORDS: 3D-structure;Acetylation;Cytoplasm;Direct protein sequencing;Disease variant;Host-virus interaction;Nucleus;Phosphoprotein;Proteasome;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Nucleus Note=Translocated from the cytoplasm into the nucleus following interaction with AKIRIN2, which bridges the proteasome with the nuclear import receptor IPO9. " P28178,"PROTEIN NAMES: Protein kinase 2 (PK2) PROTEIN FAMILY: Protein kinase superfamily, AGC Ser/Thr protein kinase family, S6 kinase subfamily ORGANISM: Dictyostelium discoideum (Social amoeba) SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily. FUNCTION: Required for morphogenesis during multicellular development. Phosphorylates talB, gefN, gefS, PI4P 5-kinase and gacQ. KEYWORDS: ATP-binding;cAMP;Cell membrane;Chemotaxis;Cytoplasm;Kinase;Lipoprotein;Membrane;Myristate;Nucleotide-binding;Phosphoprotein;Reference proteome;Serine/threonine-protein kinase;Transferase SUBCELLULAR LOCATION: Cytoplasm. Cell membrane; Lipid-anchor; Cytoplasmic side. Note=Persistently localized on the cell membrane. " P28246,"PROTEIN NAMES: Bicyclomycin resistance protein (Sulfonamide resistance protein) PROTEIN FAMILY: Major facilitator superfamily, Bcr/CmlA family ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the major facilitator superfamily. Bcr/CmlA family. FUNCTION: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase. A transporter able to export peptides. When overexpressed, allows cells deleted for multiple peptidases (pepA, pepB, pepD and pepN) to grow in the presence of dipeptides Ala-Gln or Gly-Tyr which otherwise inhibit growth. Cells overexpressing this protein have decreased intracellular levels of Ala-Gln dipeptide, and in a system that produces the Ala-Gln dipeptide overproduction of this protein increases export of the dipeptide. KEYWORDS: Antibiotic resistance;Cell inner membrane;Cell membrane;Membrane;Peptide transport;Protein transport;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell inner membrane ; Multi-pass membrane protein " P28286,"PROTEIN NAMES: 5-hydroxytryptamine receptor 2B (5-HT receptor 2B) (5-hydroxytryptamine receptor 1B) (5-HT receptor 1B) (Serotonin receptor 1B) (Serotonin receptor 2B) PROTEIN FAMILY: G-protein coupled receptor 1 family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the G-protein coupled receptor 1 family. FUNCTION: This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins which inhibit adenylate cyclase. KEYWORDS: Cell membrane;Disulfide bond;G-protein coupled receptor;Glycoprotein;Membrane;Receptor;Reference proteome;Transducer;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. " P28293,"PROTEIN NAMES: Cathepsin G (Vimentin-specific protease) (VSP) PROTEIN FAMILY: Peptidase S1 family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the peptidase S1 family. FUNCTION: Serine protease with trypsin- and chymotrypsin-like specificity. Also displays antibacterial activity against Gram-negative and Gram-positive bacteria independent of its protease activity. Prefers Phe and Tyr residues in the P1 position of substrates but also cleaves efficiently after Trp and Leu. Shows a preference for negatively charged amino acids in the P2' position and for aliphatic amino acids both upstream and downstream of the cleavage site. Required for recruitment and activation of platelets which is mediated by the F2RL3/PAR4 platelet receptor. Binds reversibly to and stimulates B cells and CD4(+) and CD8(+) T cells. Also binds reversibly to natural killer (NK) cells and enhances NK cell cytotoxicity through its protease activity. Cleaves complement C3 (By similarity). Cleaves vimentin. Cleaves thrombin receptor F2R/PAR1. Cleaves the synovial mucin-type protein PRG4/lubricin. Cleaves and activates IL36G which promotes expression of chemokines CXCL1 and CXLC8 in keratinocytes. Cleaves IL33 into mature forms which have greater activity than the unprocessed form. Cleaves coagulation factor F8 to produce a partially activated form. Also cleaves and activates coagulation factor F10. Cleaves leukocyte cell surface protein SPN/CD43 to releases its extracellular domain and trigger its intramembrane proteolysis by gamma-secretase, releasing the CD43 cytoplasmic tail chain (CD43-ct) which translocates to the nucleus. During apoptosis, cleaves SMARCA2/BRM to produce a 160 kDa cleavage product which localizes to the cytosol. Cleaves MBP in B cell lysosomes at '221-Phe-|-Lys-222', degrading the major immunogenic MBP epitope and preventing the activation of MBP-specific autoreactive T cells. Cleaves annexin ANXA1 and antimicrobial peptide CAMP to produce peptides which act on neutrophil N-formyl peptide receptors to enhance the release of CXCL2. Acts as a ligand for the N-formyl peptide receptor FPR1, enhancing phagocyte chemotaxis. Has antibacterial activity against the Gram-negative bacteria N.gonorrhoeae and P.aeruginosa. Likely to act against N.gonorrhoeae by interacting with N.gonorrhoeae penA/PBP2. Exhibits potent antimicrobial activity against the Gram-positive bacterium L.monocytogenes. Has antibacterial activity against the Gram-positive bacterium S.aureus and degrades S.aureus biofilms, allowing polymorphonuclear leukocytes to penetrate the biofilm and phagocytose bacteria. Has antibacterial activity against M.tuberculosis (By similarity). KEYWORDS: Antibiotic;Antimicrobial;Cell membrane;Chemotaxis;Cytoplasm;Direct protein sequencing;Disulfide bond;Glycoprotein;Hydrolase;Lysosome;Membrane;Nucleus;Protease;Reference proteome;Secreted;Serine protease;Signal;Zymogen SUBCELLULAR LOCATION: Cell membrane ; Peripheral membrane protein Cytoplasmic granule Secreted Cytoplasm, cytosol Lysosome Nucleus Note=Secreted by activated neutrophils. Detected in synovial fluid. Localizes to lysosomes in B cells where it is not endogenously synthesized but is internalized from the cell membrane. Localizes to the nucleus during apoptosis. " P28313,"PROTEIN NAMES: Peroxidase PROTEIN FAMILY: Peroxidase family, Ligninase subfamily ORGANISM: Arthromyces ramosus SIMILARITY: Belongs to the peroxidase family. Ligninase subfamily. KEYWORDS: 3D-structure;Calcium;Direct protein sequencing;Disulfide bond;Glycoprotein;Heme;Hydrogen peroxide;Iron;Metal-binding;Oxidoreductase;Peroxidase;Pyrrolidone carboxylic acid;Secreted;Signal SUBCELLULAR LOCATION: Secreted. " P28324,"PROTEIN NAMES: ETS domain-containing protein Elk-4 (Serum response factor accessory protein 1) (SAP-1) (SRF accessory protein 1) PROTEIN FAMILY: ETS family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the ETS family. FUNCTION: Involved in both transcriptional activation and repression. Interaction with SIRT7 leads to recruitment and stabilization of SIRT7 at promoters, followed by deacetylation of histone H3 at 'Lys-18' (H3K18Ac) and subsequent transcription repression. Forms a ternary complex with the serum response factor (SRF). Requires DNA-bound SRF for ternary complex formation and makes extensive DNA contacts to the 5'side of SRF, but does not bind DNA autonomously. KEYWORDS: 3D-structure;Activator;Alternative splicing;DNA-binding;Isopeptide bond;Nucleus;Phosphoprotein;Reference proteome;Repressor;Transcription;Transcription regulation;Ubl conjugation SUBCELLULAR LOCATION: Nucleus. " P28465,"PROTEIN NAMES: Protein Wnt-2 (dWnt-2) PROTEIN FAMILY: Wnt family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the Wnt family. FUNCTION: Binds as a ligand to a family of frizzled seven-transmembrane receptors and acts through a cascade of genes on the nucleus. Segment polarity protein. May function in gonadogenesis and limb development. Wg and Wnt2 have a role in the developing trachea and together are responsible for all dorsal trunk formation. KEYWORDS: Developmental protein;Disulfide bond;Extracellular matrix;Glycoprotein;Lipoprotein;Reference proteome;Secreted;Segmentation polarity protein;Signal;Wnt signaling pathway SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix. " P28515,"PROTEIN NAMES: Transcription factor elt-1 ORGANISM: Caenorhabditis elegans FUNCTION: Transcriptional activator that binds to the consensus sequence 5'-[AT]GATA[AG]-3' and variations thereof. During embryonic development, required for specification of cell fate of major hypodermal (epidermal) cells at the blastomere stage. The requirement is true for all four lineages derived from ABarp, ABpra and C blastomeres. Required for seam cell maintenance in late embryogenesis, for proper formation of dauer larvae and locomotion. Regulates expression of bro-1, a regulator of seam cell proliferation, via a GATA-like binding motif. Probably represses expression of eff-1 to prevent fusion of seam cells with the hypodermal syncytium. Involved in regulating expression of elt-3, a probable downstream target in hypodermal development pathways, in dorsal and ventral hypodermal cells. During postembryonic development, has a role in sperm development. Specifically, binds the bipartite consensus sequence 5'-AGATCTx(8)GATAA-3' found in genes with sperm-specific expression. Furthermore, regulates timing of developmental steps, possibly in parallel with daf-12, by controlling the expression of the let-7 family of miRNAs which in turn determines the transcription of heterochronic genes such as lin-41. KEYWORDS: Activator;Cell projection;Developmental protein;DNA-binding;Metal-binding;Nucleus;Reference proteome;Repeat;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Perikaryon Cell projection, axon Note=Expressed in pachytene nuclei during the first stage of meiosis. " P28630,"PROTEIN NAMES: DNA polymerase III subunit delta PROTEIN FAMILY: DNA polymerase HolA subunit family ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the DNA polymerase HolA subunit family. FUNCTION: Part of the beta sliding clamp loading complex, which hydrolyzes ATP to load the beta clamp onto primed DNA to form the DNA replication pre-initiation complex. DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3'-5' exonuclease activity. The delta subunit is the wrench that will open the beta subunit dimer, which has been modeled to leave a gap large enough for ssDNA to pass through. The gamma complex (gamma(3),delta,delta') is thought to load beta dimers onto DNA by binding ATP which alters the complex's conformation so it can bind beta sliding clamp dimers and open them at one interface. Primed DNA is recognized, ATP is hydrolyzed releasing the gamma complex and closing the beta sliding clamp ring around the primed DNA. KEYWORDS: 3D-structure;Direct protein sequencing;DNA replication;DNA-directed DNA polymerase;Nucleotidyltransferase;Reference proteome;Transferase " P28647,PROTEIN NAMES: Adenosine receptor A3 (TGPCR1) PROTEIN FAMILY: G-protein coupled receptor 1 family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the G-protein coupled receptor 1 family. FUNCTION: Receptor for adenosine. The activity of this receptor is mediated by G proteins which inhibits adenylyl cyclase. May play a role during reproduction. KEYWORDS: Alternative splicing;Cell membrane;Disulfide bond;G-protein coupled receptor;Glycoprotein;Lipoprotein;Membrane;Palmitate;Phosphoprotein;Receptor;Reference proteome;Transducer;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein P28651,PROTEIN NAMES: Carbonic anhydrase-related protein (CARP) (Carbonic anhydrase VIII) (CA-VIII) PROTEIN FAMILY: Alpha-carbonic anhydrase family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the alpha-carbonic anhydrase family. FUNCTION: Does not have a carbonic anhydrase catalytic activity. KEYWORDS: Direct protein sequencing;Metal-binding;Phosphoprotein;Reference proteome;Zinc P28737,"PROTEIN NAMES: Outer mitochondrial transmembrane helix translocase (Mitochondrial sorting of proteins) (Tat-binding homolog 4) PROTEIN FAMILY: AAA ATPase family, MSP1 subfamily ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the AAA ATPase family. MSP1 subfamily. FUNCTION: Outer mitochondrial translocase required to remove mislocalized tail-anchored transmembrane proteins on mitochondria. Specifically recognizes and binds exposed hydrophobic surfaces of mistargeted tail-anchored transmembrane proteins. Acts as a dislocase that mediates the ATP-dependent extraction of mistargeted tail-anchored transmembrane proteins from the mitochondrion outer membrane. Able to unfold protein substrates by processive threading through its central pore. Once extracted from the mitochondrion outer membrane, substrate proteins are then transferred to the endoplasmic reticulum, where they are ubiquitinated and degraded by the proteasome. Also mediates extraction of excess tail-anchored proteins from the peroxisomal membrane. In normal conditions, MSP1 translocase activity is inhibited by PEX3 at peroxisomes; only catalyzes removal of excess tail-anchored proteins. KEYWORDS: 3D-structure;ATP-binding;Membrane;Mitochondrion;Mitochondrion outer membrane;Nucleotide-binding;Peroxisome;Reference proteome;Translocase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Mitochondrion outer membrane ; Single-pass membrane protein Peroxisome membrane ; Single-pass membrane protein MISCELLANEOUS: Present with 3530 molecules/cell in log phase SD medium." P28797,"PROTEIN NAMES: Progranulin (PGRN) (Acrogranin) (Proepithelin) (PEPI) [Cleaved into: Paragranulin; Granulin-1; Granulin-2; Granulin-3; Granulin-4; Granulin-5; Granulin-6; Granulin-7] PROTEIN FAMILY: Granulin family ORGANISM: Cavia porcellus (Guinea pig) SIMILARITY: Belongs to the granulin family. FUNCTION: Secreted protein that acts as a key regulator of lysosomal function and as a growth factor involved in inflammation, wound healing and cell proliferation (By similarity). Regulates protein trafficking to lysosomes and, also the activity of lysosomal enzymes (By similarity). Facilitates also the acidification of lysosomes, causing degradation of mature CTSD by CTSB (By similarity). In addition, functions as a wound-related growth factor that acts directly on dermal fibroblasts and endothelial cells to promote division, migration and the formation of capillary-like tubule structures (By similarity). Also promotes epithelial cell proliferation by blocking TNF-mediated neutrophil activation preventing release of oxidants and proteases (By similarity). Moreover, modulates inflammation in neurons by preserving neurons survival, axonal outgrowth and neuronal integrity (By similarity).; FUNCTION: [Granulin-3]: Inhibits epithelial cell proliferation and induces epithelial cells to secrete IL-8.; FUNCTION: [Granulin-7]: Stabilizes CTSD through interaction with CTSD leading to maintain its aspartic-type peptidase activity. KEYWORDS: Cytokine;Direct protein sequencing;Disulfide bond;Glycoprotein;Lysosome;Reference proteome;Repeat;Secreted;Signal SUBCELLULAR LOCATION: Secreted Lysosome Note=Endocytosed by SORT1 and delivred to lysosomes. Targeted to lysosome by PSAP via M6PR and LRP1, in both biosynthetic and endocytic pathways (By similarity). Co-localized with GBA1 in the intracellular trafficking compartments until to lysosome (By similarity). " P28820,"PROTEIN NAMES: Aminodeoxychorismate synthase component 1 (ADC synthase) (ADCS) (4-amino-4-deoxychorismate synthase component 1) PROTEIN FAMILY: Anthranilate synthase component I family ORGANISM: Bacillus subtilis (strain 168) SIMILARITY: Belongs to the anthranilate synthase component I family. FUNCTION: Part of a heterodimeric complex that catalyzes the two-step biosynthesis of 4-amino-4-deoxychorismate (ADC), a precursor of p-aminobenzoate (PABA) and tetrahydrofolate. In the first step, a glutamine amidotransferase (PabA) generates ammonia as a substrate that, along with chorismate, is used in the second step, catalyzed by aminodeoxychorismate synthase (PabB) to produce ADC. PATHWAY: Cofactor biosynthesis; tetrahydrofolate biosynthesis; 4-aminobenzoate from chorismate: step 1/2. KEYWORDS: 3D-structure;Folate biosynthesis;Magnesium;Reference proteome;Transferase MISCELLANEOUS: The catalytically active amino acid residue K274 of E.coli enzyme is missing and corresponds to A283 in B.subtilis. It is postulated that the enzymatic mechanism for the PABA biosynthesis in B.subtilis proceeds without covalent intermediate. First, ammonia is added at C2 of chorismate with concomitant loss of the C4 hydroxy group, yielding 2-amino-2-deoxyisochorismate (ADIC). The second step is the addition of a second molecule ammonia to C4 of ADIC with concomitant loss of the C2 amino group, yielding ADC. Both steps are catalyzed by ADCS." P28824,"PROTEIN NAMES: Neuropilin-1 (A5 antigen) (A5 protein) PROTEIN FAMILY: Neuropilin family ORGANISM: Xenopus laevis (African clawed frog) SIMILARITY: Belongs to the neuropilin family. FUNCTION: Receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system (By similarity). Mediates the chemorepulsant activity of semaphorins. Binding to VEGFA initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (By similarity). Regulates mitochondrial iron transport via interaction (By similarity). KEYWORDS: Angiogenesis;Calcium;Cell membrane;Developmental protein;Differentiation;Disulfide bond;Glycoprotein;Heparan sulfate;Heparin-binding;Membrane;Metal-binding;Mitochondrion;Neurogenesis;Proteoglycan;Receptor;Reference proteome;Repeat;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Mitochondrion membrane ; Single-pass type I membrane protein Cell membrane ; Single-pass type I membrane protein " P28826,"PROTEIN NAMES: Meprin A subunit beta (Endopeptidase-2) (Meprin B) ORGANISM: Rattus norvegicus (Rat) FUNCTION: Membrane metallopeptidase that sheds many membrane-bound proteins. Exhibits a strong preference for acidic amino acids at the P1' position. Known substrates include: FGF19, VGFA, IL1B, IL18, procollagen I and III, E-cadherin, KLK7, gastrin, ADAM10, tenascin-C. The presence of several pro-inflammatory cytokine among substrates implicate MEP1B in inflammation. It is also involved in tissue remodeling due to its capability to degrade extracellular matrix components. KEYWORDS: Cell membrane;Direct protein sequencing;Disulfide bond;EGF-like domain;Glycoprotein;Hydrolase;Inflammatory response;Membrane;Metal-binding;Metalloprotease;Protease;Reference proteome;Secreted;Signal;Transmembrane;Transmembrane helix;Zinc;Zymogen SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein Secreted Note=Homodimers are essentially membrane bound but may also be shed from the surface by ADAM-10 and ADAM-17. " P28908,PROTEIN NAMES: Tumor necrosis factor receptor superfamily member 8 (CD30L receptor) (Ki-1 antigen) (Lymphocyte activation antigen CD30) (CD antigen CD30) PROTEIN FAMILY: TNFR8 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the TNFR8 family. FUNCTION: Receptor for TNFSF8/CD30L. May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa-B. KEYWORDS: 3D-structure;Alternative initiation;Alternative splicing;Cell membrane;Cytoplasm;Disulfide bond;Glycoprotein;Membrane;Phosphoprotein;Receptor;Reference proteome;Repeat;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: [Isoform 1]: Cell membrane ; Single-pass type I membrane protein.; SUBCELLULAR LOCATION: [Isoform 2]: Cytoplasm MISCELLANEOUS: Most specific Hodgkin disease associated antigen. P29056,PROTEIN NAMES: Transcription factor IIIB 70 kDa subunit (TFIIIB) (B-related factor 1) (BRF-1) PROTEIN FAMILY: TFIIB family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the TFIIB family. FUNCTION: General activator of RNA polymerase III transcription. Interacts with TBP. Binds to Pol III subunit C34 and to the TAU135 component of TFIIIC. KEYWORDS: 3D-structure;Activator;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Repeat;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus. MISCELLANEOUS: Present with 4330 molecules/cell in log phase SD medium. P29082,"PROTEIN NAMES: Sulfur oxygenase/reductase (Sulfur oxygenase reductase) (SOR) ORGANISM: Acidianus ambivalens (Desulfurolobus ambivalens) FUNCTION: Catalyzes the simultaneous oxidation and reduction of elemental sulfur in the presence of oxygen, with sulfite and hydrogen sulfide as products. KEYWORDS: 3D-structure;Cytoplasm;Direct protein sequencing;Iron;Metal-binding;Oxidoreductase SUBCELLULAR LOCATION: Cytoplasm. " P29241,"PROTEIN NAMES: ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase (2'-phospho-ADP-ribosyl cyclase) (2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase) (2'-phospho-cyclic-ADP-ribose transferase) (ADP-ribosyl cyclase) (ADPRC) (ADRC) (NAD glycohydrolase) (NAD(+) nucleosidase) (NADase) PROTEIN FAMILY: ADP-ribosyl cyclase family ORGANISM: Aplysia californica (California sea hare) SIMILARITY: Belongs to the ADP-ribosyl cyclase family. FUNCTION: Synthesizes cyclic ADP-ribose (cADPR), a second messenger for calcium mobilization from endoplasmic reticulum; ADP-ribose is a minor product. Synthesizes the Ca(2+) mobilizer nicotinate-adenine dinucleotide phosphate from 2'-phospho-cADPR and nicotinic acid as well as from NADP(+) and nicotinic acid; with NADP(+) as substrate preferentially catalyzes NADP(+) hydrolysis rather than NAADP(+) synthesis, about 70-fold better at pH 7.4. Has cADPR hydrolase activity at very high enzyme concentrations, which is probably not physiological. The conversion of NAD(+) into ADP-ribose is also only observed at high enzyme concentrations and results from the hydrolysis of cADP-ribose. KEYWORDS: 3D-structure;Calcium;Cytoplasmic vesicle;Direct protein sequencing;Disulfide bond;Fertilization;Hydrolase;NAD;NADP;Phosphoprotein;Signal;Transferase SUBCELLULAR LOCATION: Cytoplasmic vesicle Note=Localized to vesicles or granules within ova of all stages. " P29293,"PROTEIN NAMES: Acrosin [Cleaved into: Acrosin light chain; Acrosin heavy chain] PROTEIN FAMILY: Peptidase S1 family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the peptidase S1 family. FUNCTION: Acrosin is the major protease of mammalian spermatozoa. It is a serine protease of trypsin-like cleavage specificity, it is synthesized in a zymogen form, proacrosin and stored in the acrosome. KEYWORDS: Disulfide bond;Glycoprotein;Hydrolase;Protease;Reference proteome;Serine protease;Signal;Zymogen " P29295,"PROTEIN NAMES: Casein kinase I homolog HRR25 PROTEIN FAMILY: Protein kinase superfamily, CK1 Ser/Thr protein kinase family, Casein kinase I subfamily ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily. FUNCTION: Protein kinase which phosphorylates serine and threonine residues. Can use casein as a substrate. Phosphorylates elongator complex member ELP1/IKI3 on 'Ser-1198' and 'Ser-1202' which promotes the tRNA modification function of the complex. Associated with repair of damaged DNA and meiosis. KEYWORDS: 3D-structure;ATP-binding;Cytoplasm;DNA damage;DNA repair;Kinase;Nucleotide-binding;Nucleus;Phosphoprotein;Reference proteome;Serine/threonine-protein kinase;Transferase SUBCELLULAR LOCATION: Cytoplasm Nucleus, nucleolus Nucleus, nucleoplasm MISCELLANEOUS: Present with 10300 molecules/cell in log phase SD medium." P29353,"PROTEIN NAMES: SHC-transforming protein 1 (SHC-transforming protein 3) (SHC-transforming protein A) (Src homology 2 domain-containing-transforming protein C1) (SH2 domain protein C1) ORGANISM: Homo sapiens (Human) FUNCTION: Signaling adapter that couples activated growth factor receptors to signaling pathways. Participates in a signaling cascade initiated by activated KIT and KITLG/SCF. Isoform p46Shc and isoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals. Isoform p46Shc and isoform p52Shc may thus function as initiators of the Ras signaling cascade in various non-neuronal systems. Isoform p66Shc does not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular response to oxidative stress and life span. Isoform p66Shc acts as a downstream target of the tumor suppressor p53 and is indispensable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome c release and apoptosis. The expression of isoform p66Shc has been correlated with life span (By similarity). Participates in signaling downstream of the angiopoietin receptor TEK/TIE2, and plays a role in the regulation of endothelial cell migration and sprouting angiogenesis. KEYWORDS: 3D-structure;Acetylation;Alternative promoter usage;Alternative splicing;Angiogenesis;Cell junction;Cytoplasm;Growth regulation;Host-virus interaction;Mitochondrion;Phosphoprotein;Reference proteome;SH2 domain SUBCELLULAR LOCATION: Cytoplasm. Cell junction, focal adhesion.; SUBCELLULAR LOCATION: [Isoform p46Shc]: Mitochondrion matrix Note=Localized to the mitochondria matrix. Targeting of isoform p46Shc to mitochondria is mediated by its first 32 amino acids, which behave as a bona fide mitochondrial targeting sequence. Isoform p52Shc and isoform p66Shc, that contain the same sequence but more internally located, display a different subcellular localization.; SUBCELLULAR LOCATION: [Isoform p66Shc]: Mitochondrion Note=In case of oxidative conditions, phosphorylation at 'Ser-36' of isoform p66Shc, leads to mitochondrial accumulation. MISCELLANEOUS: [Isoform p66Shc]: Regulated by epigenetic modifications of its promoter region.; MISCELLANEOUS: [Isoform 5]: Produced by alternative splicing.; MISCELLANEOUS: [Isoform 6]: Produced by alternative splicing.; MISCELLANEOUS: [Isoform 7]: Produced by alternative splicing." P29366,"PROTEIN NAMES: Bud emergence protein 1 (Suppressor of RHO3 protein 1) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Necessary for cell polarization during vegetative growth. May link the cytoskeleton to morphogenic determinants on the cell surface. KEYWORDS: 3D-structure;Acetylation;Cytoplasm;Cytoskeleton;Phosphoprotein;Reference proteome;Repeat;SH3 domain SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton. MISCELLANEOUS: Present with 6490 molecules/cell in log phase SD medium." P29468,"PROTEIN NAMES: Poly(A) polymerase (PAP) (Polynucleotide adenylyltransferase) PROTEIN FAMILY: Poly(A) polymerase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the poly(A) polymerase family. FUNCTION: Polymerase component of the cleavage and polyadenylation factor (CPF) complex, which plays a key role in polyadenylation-dependent pre-mRNA 3'-end formation and cooperates with cleavage factors including the CFIA complex and NAB4/CFIB. KEYWORDS: 3D-structure;ATP-binding;Direct protein sequencing;Magnesium;Manganese;Metal-binding;mRNA processing;Nucleotide-binding;Nucleus;Phosphoprotein;Reference proteome;RNA-binding;Transferase SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: Present with 17100 molecules/cell in log phase SD medium." P29518,"PROTEIN NAMES: Adenine nucleotide transporter BT1, chloroplastic/amyloplastic/mitochondrial (Protein brittle-1) PROTEIN FAMILY: Mitochondrial carrier family ORGANISM: Zea mays (Maize) SIMILARITY: Belongs to the mitochondrial carrier (TC 2.A.29) family. FUNCTION: Probable adenylate translocator that mediates transport of ADP-glucose into endosperm storage plastids during starch synthesis. Transports cytosolic ADP-glucose to amyloplast stroma by counter-exchange with ADP. KEYWORDS: Amyloplast;Chloroplast;Membrane;Mitochondrion;Mitochondrion inner membrane;Plastid;Plastid inner membrane;Reference proteome;Repeat;Transit peptide;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Plastid, chloroplast inner membrane ; Multi-pass membrane protein Plastid, amyloplast inner membrane ; Multi-pass membrane protein Mitochondrion inner membrane ; Multi-pass membrane protein Note=Dually targeted to mitochondria and plastids. The N-terminal extension acts as a plastidic transit peptide. Dual localization of BT1 does not seem to be due to alternative transcription start sites, translation initiation sites or alternative exon splicing. " P29549,"PROTEIN NAMES: Telomere-binding protein subunit alpha (TEBP alpha) (Telomere-binding protein 56 kDa subunit) PROTEIN FAMILY: Telombin family ORGANISM: Sterkiella nova (Ciliate) (Oxytricha nova) SIMILARITY: Belongs to the telombin family. FUNCTION: May function as protective capping of the single-stranded telomeric overhang. May also participate in telomere length regulation during DNA replication. Binds specifically to the T4G4-containing extension on the 3'strand and protects this region of the telomere from nuclease digestion and chemical modification. KEYWORDS: 3D-structure;Chromosome;Direct protein sequencing;DNA-binding;Nucleus;Telomere SUBCELLULAR LOCATION: Nucleus. Chromosome, telomere. MISCELLANEOUS: The sequence of the A (or alanine) version is shown. The S (or serine) version differs in only two positions, and the K version in only one. There may be other versions in addition to the S, A, and K versions." P29594,"PROTEIN NAMES: Caspase-2 (CASP-2) (Neural precursor cell expressed developmentally down-regulated protein 2) (NEDD-2) (Protease ICH-1) [Cleaved into: Caspase-2 subunit p18; Caspase-2 subunit p13; Caspase-2 subunit p12] PROTEIN FAMILY: Peptidase C14A family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the peptidase C14A family. FUNCTION: Involved in the activation cascade of caspases responsible for apoptosis execution. Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival. Associates with PIDD1 and CRADD to form the PIDDosome, a complex that activates CASP2 and triggers apoptosis in response to genotoxic stress (By similarity). KEYWORDS: Acetylation;Apoptosis;Hydrolase;Phosphoprotein;Protease;Reference proteome;Thiol protease;Zymogen " P29622,"PROTEIN NAMES: Kallistatin (Kallikrein inhibitor) (Peptidase inhibitor 4) (PI-4) (Serpin A4) PROTEIN FAMILY: Serpin family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the serpin family. FUNCTION: Inhibits human amidolytic and kininogenase activities of tissue kallikrein. Inhibition is achieved by formation of an equimolar, heat- and SDS-stable complex between the inhibitor and the enzyme, and generation of a small C-terminal fragment of the inhibitor due to cleavage at the reactive site by tissue kallikrein. KEYWORDS: 3D-structure;Direct protein sequencing;Glycoprotein;Protease inhibitor;Reference proteome;Secreted;Serine protease inhibitor;Signal SUBCELLULAR LOCATION: Secreted. MISCELLANEOUS: Heparin blocks kallistatin's complex formation with tissue kallikrein and abolishes its inhibitory effect on tissue kallikrein's activity." P29723,"PROTEIN NAMES: Putative DD-carboxypeptidase TP_0574 (47 kDa lipoprotein) (Tp47) (Tpp47) (47 kDa membrane antigen) (47-kilodalton major integral membrane immunogen) ORGANISM: Treponema pallidum (strain Nichols) FUNCTION: A possible D,D-carboxypeptidase, that releases amino acids sequentially from a proteins C-terminus. Has zinc-dependent carboxypeptidase activity on synthetic depsipeptide substrates. May serve to decrease cross-linking of peptidoglycan, promoting the highly sinusous motility of this spirochaete (Probable). Overexpression of the whole protein in E.coli leads to aberrant cell morphology and extrusion of the cytoplasm, while overexpression of a construct with the first 62 resides of the protein fused to PhoA does have this effect, suggesting the whole protein, not the lipoprotein moiety, is toxic. Binds penicillin. Penicillin binding is covalent, does not require lipidation, and is zinc-dependent. While this protein has beta-lactamase activity in vitro, that is probably not its role in vivo, as T.pallidum is very sensitive to penicillin antibiotics.; FUNCTION: A pathogen-specific membrane antigen. Most abundant of the membrane lipoproteins, only found in pathogenic treponemes, suggesting that it is an important structural moiety in the cell envelope of virulent treponemal subspecies. A lipopeptide corresponding to the first 6 mature residues induces host (human and mouse) cytokine release by monocyte cell lines via TLR2 and CD14; nonlipidated protein does not stimulate host cells. Stimulates host (human) dendritic cell maturation to become MHC class II-positive antigen presenting cells via TLR2, which depends on lipidation; nonlipidated protein does not stimulate maturation. KEYWORDS: 3D-structure;Cell inner membrane;Cell membrane;Direct protein sequencing;Hydrolase;Lipoprotein;Membrane;Palmitate;Protease;Reference proteome;Signal SUBCELLULAR LOCATION: Cell inner membrane ; Lipid-anchor MISCELLANEOUS: A recombinant non-lipidated form (residues 20-434) is recognized by human antisera, indicating the lipidation site is not essential for antigenicity, although the acylated lipopeptide clearly is antigenic. The non-lipidated form binds also penicillin." P29747,PROTEIN NAMES: Cyclic AMP response element-binding protein A (cAMP response element-binding protein A) (dCREB-A) (Box B-binding factor 2) (BBF-2) PROTEIN FAMILY: BZIP family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the bZIP family. FUNCTION: Transcriptional activator. Binds to fat body-specific enhancers of alcohol dehydrogenase (ADH) and yolk protein genes. BBF-2 may play a role in fat body gene expression. It binds the consensus sequence 5'-T[AC]NACGTAN[TG]C-3'. KEYWORDS: Activator;DNA-binding;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus. P29768,PROTEIN NAMES: Sialidase (N-acylneuraminate glycohydrolase) (Neuraminidase) (NANase) (STNA) PROTEIN FAMILY: Glycosyl hydrolase 33 family ORGANISM: Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) SIMILARITY: Belongs to the glycosyl hydrolase 33 family. FUNCTION: Cleaves the terminal sialic acid (N-acetyl neuraminic acid) from carbohydrate chains in glycoproteins providing free sialic acid which can be used as carbon and energy sources. Sialidases have been suggested to be pathogenic factors in microbial infections. KEYWORDS: 3D-structure;Direct protein sequencing;Disulfide bond;Glycosidase;Hydrolase;Reference proteome;Repeat P29957,"PROTEIN NAMES: Alpha-amylase (1,4-alpha-D-glucan glucanohydrolase) PROTEIN FAMILY: Glycosyl hydrolase 13 family ORGANISM: Pseudoalteromonas haloplanktis (Alteromonas haloplanktis) SIMILARITY: Belongs to the glycosyl hydrolase 13 family. KEYWORDS: 3D-structure;Calcium;Carbohydrate metabolism;Chloride;Direct protein sequencing;Disulfide bond;Glycosidase;Hydrolase;Metal-binding;Secreted;Signal SUBCELLULAR LOCATION: Secreted " P30039,PROTEIN NAMES: Phenazine biosynthesis-like domain-containing protein (MAWD-binding protein) (MAWDBP) (Unknown protein 32 from 2D-page of liver tissue) PROTEIN FAMILY: PhzF family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the PhzF family. KEYWORDS: Alternative splicing;Direct protein sequencing;Isomerase;Reference proteome P30082,"PROTEIN NAMES: Glucagon receptor (GL-R) PROTEIN FAMILY: G-protein coupled receptor 2 family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the G-protein coupled receptor 2 family. FUNCTION: G-protein coupled receptor for glucagon that plays a central role in the regulation of blood glucose levels and glucose homeostasis. Regulates the rate of hepatic glucose production by promoting glycogen hydrolysis and gluconeogenesis. Plays an important role in mediating the responses to fasting. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Promotes activation of adenylate cyclase. Besides, plays a role in signaling via a phosphatidylinositol-calcium second messenger system. KEYWORDS: Cell membrane;Disulfide bond;G-protein coupled receptor;Glycoprotein;Membrane;Phosphoprotein;Receptor;Reference proteome;Signal;Transducer;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Note=Is rapidly internalized after ligand-binding. " P30120,"PROTEIN NAMES: Metalloproteinase inhibitor 1 (Tissue inhibitor of metalloproteinases 1) (TIMP-1) PROTEIN FAMILY: Protease inhibitor I35 (TIMP) family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the protease inhibitor I35 (TIMP) family. FUNCTION: Metalloproteinase inhibitor that functions by forming one to one complexes with target metalloproteinases, such as collagenases, and irreversibly inactivates them by binding to their catalytic zinc cofactor. Acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP13 and MMP16. Does not act on MMP14. Also functions as a growth factor that regulates cell differentiation, migration and cell death and activates cellular signaling cascades via CD63 and ITGB1. Plays a role in integrin signaling. Also stimulates steroidogenesis by Leydig and ovarian granuloma cells; procathepsin L is required for maximal activity. KEYWORDS: Direct protein sequencing;Disulfide bond;Glycoprotein;Growth factor;Metal-binding;Metalloenzyme inhibitor;Metalloprotease inhibitor;Phosphoprotein;Protease inhibitor;Reference proteome;Secreted;Signal;Steroidogenesis;Zinc SUBCELLULAR LOCATION: Secreted " P30290,"PROTEIN NAMES: Mitosis inhibitor protein kinase mik1 PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family, WEE1 subfamily ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily. FUNCTION: Protein kinase that acts both on serines and on tyrosines. It acts as a negative regulator of entry into mitosis (G2 to M transition). Phosphorylates and inhibits cdc2. KEYWORDS: ATP-binding;Cell cycle;Cell division;Kinase;Magnesium;Metal-binding;Mitosis;Nucleotide-binding;Reference proteome;Serine/threonine-protein kinase;Transferase;Tyrosine-protein kinase " P30429,"PROTEIN NAMES: Cell death protein 4 ORGANISM: Caenorhabditis elegans FUNCTION: Component of the egl-1, ced-9, ced-4 and ced-3 apoptotic signaling cascade required for the initiation of programmed cell death in cells fated to die during embryonic and postembryonic development. During oogenesis, required for germline apoptosis downstream of ced-9 and upstream of ced-3 but independently of egl-1. May regulate germline apoptosis in response to DNA damage, probably downstream of let-60/ras and mpk-1 pathway. Regulates CEP neuron apoptosis in response to high Al(3+) levels. During male tail morphogenesis, promotes apoptosis of the tail-spike cell upstream of ced-3 but independently of egl-1 and ced-9. May play a role in sex-specific cell apoptosis, probably by promoting ced-3-mediated cleavage of sex-determining protein fem-1. During larval development, required for the elimination of transient presynaptic components downstream of egl-1 and ced-9 and upstream of ced-3 apoptotic pathway. Downstream of calreticulin crt-1 and upstream of ced-3 and independently of egl-1 and ced-9, plays a role in the initial steps of axonal regrowth following axotomy. Together with ain-1, a component of the miRNA-induced-silencing complex (miRISC), and probably upstream of ced-3, regulates temporal cell fate patterning during larval development. May play a role in resistance to S.typhimurium-mediated infection.; FUNCTION: [Isoform a]: Plays a major role in programmed cell death. egl-1 binds to and directly inhibits the activity of ced-9, releasing the cell death activator ced-4 from a ced-9/ced-4 containing protein complex and allowing ced-4 to induce caspase ced-3 autoproteolytic cleavage and activation. Also forms a holoenzyme with processed ced-3 enhancing ced-3 activity.; FUNCTION: [Isoform b]: Prevents programmed cell death. KEYWORDS: 3D-structure;Alternative splicing;Apoptosis;ATP-binding;Cytoplasm;Magnesium;Metal-binding;Mitochondrion;Nucleotide-binding;Reference proteome SUBCELLULAR LOCATION: Mitochondrion Cytoplasm, perinuclear region Note=In non cell death induced cells, ced-9 is required for mitochondrial localization. Perinuclear in cell death induced cells. " P30519,"PROTEIN NAMES: Heme oxygenase 2 (HO-2) [Cleaved into: Heme oxygenase 2 soluble form] PROTEIN FAMILY: Heme oxygenase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the heme oxygenase family. FUNCTION: [Heme oxygenase 2]: Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron.; FUNCTION: [Heme oxygenase 2 soluble form]: Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron. KEYWORDS: 3D-structure;Acetylation;Alternative splicing;Direct protein sequencing;Endoplasmic reticulum;Heme;Iron;Membrane;Metal-binding;Microsome;Oxidoreductase;Phosphoprotein;Reference proteome;Repeat;S-nitrosylation;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Microsome membrane ; Single-pass type IV membrane protein ; Cytoplasmic side Endoplasmic reticulum membrane ; Single-pass type IV membrane protein ; Cytoplasmic side " P30530,"PROTEIN NAMES: Tyrosine-protein kinase receptor UFO (AXL oncogene) PROTEIN FAMILY: Protein kinase superfamily, Tyr protein kinase family, AXL/UFO subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily. FUNCTION: Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding growth factor GAS6 and which is thus regulating many physiological processes including cell survival, cell proliferation, migration and differentiation. Ligand binding at the cell surface induces dimerization and autophosphorylation of AXL. Following activation by ligand, AXL binds and induces tyrosine phosphorylation of PI3-kinase subunits PIK3R1, PIK3R2 and PIK3R3; but also GRB2, PLCG1, LCK and PTPN11. Other downstream substrate candidates for AXL are CBL, NCK2, SOCS1 and TNS2. Recruitment of GRB2 and phosphatidylinositol 3 kinase regulatory subunits by AXL leads to the downstream activation of the AKT kinase. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses. Also plays an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response.; FUNCTION: (Microbial infection) Acts as a receptor for lassa virus and lymphocytic choriomeningitis virus, possibly through GAS6 binding to phosphatidyl-serine at the surface of virion envelope.; FUNCTION: (Microbial infection) Acts as a receptor for Ebolavirus, possibly through GAS6 binding to phosphatidyl-serine at the surface of virion envelope.; FUNCTION: (Microbial infection) Promotes Zika virus entry in glial cells, Sertoli cells and astrocytes. Additionally, Zika virus potentiates AXL kinase activity to antagonize type I interferon signaling and thereby promotes infection. Interferon signaling inhibition occurs via an SOCS1-dependent mechanism. KEYWORDS: 3D-structure;Alternative splicing;ATP-binding;Cell membrane;Differentiation;Disulfide bond;Glycoprotein;Host cell receptor for virus entry;Host-virus interaction;Immunity;Immunoglobulin domain;Innate immunity;Kinase;Membrane;Nucleotide-binding;Oncogene;Phosphoprotein;Proto-oncogene;Receptor;Reference proteome;Repeat;Signal;Transferase;Transmembrane;Transmembrane helix;Tyrosine-protein kinase;Ubl conjugation SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein " P30546,"PROTEIN NAMES: Histamine H1 receptor (H1R) (HH1R) PROTEIN FAMILY: G-protein coupled receptor 1 family ORGANISM: Bos taurus (Bovine) SIMILARITY: Belongs to the G-protein coupled receptor 1 family. FUNCTION: In peripheral tissues, the H1 subclass of histamine receptors mediates the contraction of smooth muscles, increase in capillary permeability due to contraction of terminal venules, and catecholamine release from adrenal medulla, as well as mediating neurotransmission in the central nervous system. KEYWORDS: Cell membrane;Disulfide bond;G-protein coupled receptor;Glycoprotein;Membrane;Phosphoprotein;Receptor;Reference proteome;Transducer;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein " P30624,"PROTEIN NAMES: Long-chain-fatty-acid--CoA ligase 1 (Fatty acid activator 1) (Fatty acyl-CoA synthetase) (ACS) (FACS) (Long-chain acyl-CoA synthetase 1) PROTEIN FAMILY: ATP-dependent AMP-binding enzyme family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the ATP-dependent AMP-binding enzyme family. FUNCTION: Activates long-chain fatty acids (LCFA) by esterification of the fatty acids into metabolically active CoA-thioesters for subsequent degradation or incorporation into phospholipids. Also facilitates the transport of LCFAs into the cell, either by active transport or by decreasing the intracellular LCFA concentration. It may supplement intracellular myristoyl-CoA pools from exogenous myristate. Preferentially acts on C12:0-C16:0 fatty acids with myristic and pentadecanic acid (C15:0) having the highest activities. Also involved in long-chain base (LCB) uptake of sphingolipids. In contrast ot LCFA uptake, LCB uptake does not require ATP, suggesting that the enzyme is directly involved in active LCB uptake. Involved in the sphingolipid-to-glycerolipid metabolic pathway, converting the sphingolipid metabolite hexadecenoic acid to hexadecenoyl-CoA, which is then further converted to glycerolipids. KEYWORDS: ATP-binding;Cell membrane;Direct protein sequencing;Fatty acid metabolism;Isopeptide bond;Ligase;Lipid droplet;Lipid metabolism;Magnesium;Membrane;Nucleotide-binding;Reference proteome;Ubl conjugation SUBCELLULAR LOCATION: Lipid droplet Cell membrane MISCELLANEOUS: Present with 7470 molecules/cell in log phase SD medium." P30628,"PROTEIN NAMES: V-type proton ATPase 116 kDa subunit a 1 (V-ATPase 116 kDa isoform a 1) (Uncoordinated protein 32) (Vacuolar proton translocating ATPase 116 kDa subunit a 1) PROTEIN FAMILY: V-ATPase 116 kDa subunit family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the V-ATPase 116 kDa subunit family. FUNCTION: Subunit of the V0 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons (By similarity). V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment (By similarity). Required for assembly and activity of the vacuolar ATPase (By similarity). Regulates the size of gut granules during embryonic development. In neurons, required for necrotic cell death by promoting intracellular acidification. Required for cell death induced by hypoxia. Required for acidification of synaptic vesicles and the release of neurotransmitters from adult neurons. KEYWORDS: Alternative splicing;Hydrogen ion transport;Ion transport;Membrane;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Membrane ; Multi-pass membrane protein " P30630,"PROTEIN NAMES: Protein lin-9 (Abnormal cell lineage protein 9) PROTEIN FAMILY: Lin-9 family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the lin-9 family. FUNCTION: Synthetic multivulva class B (synMuvB) protein. SynMuvB proteins are required to repress the induction of vulval development by Ras signaling and probably act by forming the multiprotein DRM complex that represses transcription. Required for the development of sheath cells in the hermaphrodite gonad and for the development of the male spicule, rays and gonad. In association with the zinc finger protein ztf-11, negatively regulates the expression of non-neuronal genes during neurogenesis. KEYWORDS: Alternative splicing;Developmental protein;Nucleus;Reference proteome;Repressor;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " P30656,"PROTEIN NAMES: Proteasome subunit beta type-5 (Macropain subunit PRE2) (Multicatalytic endopeptidase complex subunit PRE2) (Proteasome component PRE2) (Proteinase YSCE subunit PRE2) PROTEIN FAMILY: Peptidase T1B family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the peptidase T1B family. FUNCTION: The proteasome degrades poly-ubiquitinated proteins in the cytoplasm and in the nucleus. It is essential for the regulated turnover of proteins and for the removal of misfolded proteins. The proteasome is a multicatalytic proteinase complex that is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. It has an ATP-dependent proteolytic activity. This unit is responsible of the chymotrypsin-like activity of the proteasome and is one of the principal target of the proteasome inhibitor bortezomib.; FUNCTION: This subunit is necessary for chymotryptic activity and degradation of ubiquitinated proteins. KEYWORDS: 3D-structure;Cytoplasm;Direct protein sequencing;Hydrolase;Nucleus;Protease;Proteasome;Reference proteome;Threonine protease;Zymogen SUBCELLULAR LOCATION: Cytoplasm. Nucleus. MISCELLANEOUS: The side chain of Thr-76 acts as a nucleophile, and the N-terminal amino group acts as a proton acceptor.; MISCELLANEOUS: Present with 8530 molecules/cell in log phase SD medium." P30657,"PROTEIN NAMES: Proteasome subunit beta type-7 (Macropain subunit PRE4) (Multicatalytic endopeptidase complex subunit PRE4) (Proteasome component PRE4) (Proteinase YSCE subunit PRE4) PROTEIN FAMILY: Peptidase T1B family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the peptidase T1B family. FUNCTION: Non-catalytic component of the proteasome which degrades poly-ubiquitinated proteins in the cytoplasm and in the nucleus. It is essential for the regulated turnover of proteins and for the removal of misfolded proteins. The proteasome is a multicatalytic proteinase complex that is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. It has an ATP-dependent proteolytic activity. PRE3 and PRE4 are necessary for the peptidyl-glutamyl-peptide-hydrolyzing activity. KEYWORDS: 3D-structure;Cytoplasm;Nucleus;Proteasome;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Nucleus MISCELLANEOUS: Present with 16900 molecules/cell in log phase SD medium." P30665,"PROTEIN NAMES: DNA replication licensing factor MCM4 (Cell division control protein 54) PROTEIN FAMILY: MCM family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the MCM family. FUNCTION: Acts as a component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Once loaded onto DNA, double hexamers can slide on dsDNA in the absence of ATPase activity. Required for S phase execution. KEYWORDS: 3D-structure;ATP-binding;Cell cycle;DNA replication;DNA-binding;Helicase;Hydrolase;Nucleotide-binding;Nucleus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: Present with 8800 molecules/cell in log phase SD medium.; MISCELLANEOUS: Early fractionation of eukaryotic MCM proteins yielded a variety of dimeric, trimeric and tetrameric complexes with unclear biological significance. Specifically a MCM467 subcomplex is shown to have in vitro helicase activity which is inhibited by the MCM2 subunit. The MCM2-7 hexamer is the proposed physiological active complex." P30731,"PROTEIN NAMES: G-protein coupled receptor 83 (Glucocorticoid-induced receptor) PROTEIN FAMILY: G-protein coupled receptor 1 family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the G-protein coupled receptor 1 family. FUNCTION: G-protein coupled receptor for PEN, a neuropeptide produced from the precursor protein, proSAAS (encoded by PCSK1N). Acts through a G(i)- and G(q)-alpha-alpha-mediated pathway in response to PEN. Plays a role in food intake and body weight regulation. May contribute to the regulation of anxiety-related behaviors. KEYWORDS: Alternative splicing;Cell membrane;Disulfide bond;G-protein coupled receptor;Glycoprotein;Membrane;Receptor;Reference proteome;Signal;Transducer;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Note=Colocalizes with GPR171 in the paraventricular nucleus. Colocalizes with the ghrelin receptor GHSR1A in the hypothalamus. " P30878,"PROTEIN NAMES: Melibiose permease (Melibiose carrier) (Melibiose transporter) (Melibiose/cation symporter) (Na+ (Li+)/melibiose symporter) (Thiomethylgalactoside permease II) PROTEIN FAMILY: Sodium:galactoside symporter family ORGANISM: Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) SIMILARITY: Belongs to the sodium:galactoside symporter (TC 2.A.2) family. FUNCTION: Mediates the transport of melibiose and other galactosides by a symport mechanism. Can use sodium, lithium and protons as coupling cations, with a preference for sodium and lithium. The use of Na(+) as coupling ion for sugar transport is based not on ion selectivity but on competitive binding under physiological conditions, because of a much higher Na(+) concentration under physiological conditions. KEYWORDS: 3D-structure;Cell inner membrane;Cell membrane;Membrane;Reference proteome;Sugar transport;Symport;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell inner membrane ; Multi-pass membrane protein " P30935,PROTEIN NAMES: Somatostatin receptor type 3 (SS-3-R) (SS3-R) (SS3R) (SSR-28) PROTEIN FAMILY: G-protein coupled receptor 1 family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the G-protein coupled receptor 1 family. FUNCTION: Receptor for somatostatin-14 and -28. This receptor is coupled via pertussis toxin sensitive G proteins to inhibition of adenylyl cyclase. KEYWORDS: Cell membrane;Disulfide bond;G-protein coupled receptor;Glycoprotein;Membrane;Phosphoprotein;Receptor;Reference proteome;Transducer;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Note=Internalized into endoplasmic vesicles upon somatostatin-stimulation. P31005,PROTEIN NAMES: NAD-dependent methanol dehydrogenase (MDH) (MEDH) (Type 3 alcohol dehydrogenase) PROTEIN FAMILY: Iron-containing alcohol dehydrogenase family ORGANISM: Bacillus methanolicus SIMILARITY: Belongs to the iron-containing alcohol dehydrogenase family. FUNCTION: Catalyzes the oxidation of methanol to yield formaldehyde. It possesses a NADH-dependent formaldehyde reductase activity and cannot use NADP. PATHWAY: One-carbon metabolism; methanol degradation; formaldehyde from methanol: step 1/1. KEYWORDS: Cytoplasm;Direct protein sequencing;Magnesium;Methanol utilization;NAD;Oxidoreductase;Zinc SUBCELLULAR LOCATION: Cytoplasm P31007,"PROTEIN NAMES: Disks large 1 tumor suppressor protein (Dlg) PROTEIN FAMILY: MAGUK family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the MAGUK family. FUNCTION: During embryonic development, some isoforms are essential for proper neuronal differentiation and organization. Required for cell polarity; maintenance of apicobasal polarity. Plays a critical role at septate junctions in cellular growth control during larval development. The presence of a guanylate kinase domain suggests involvement in cellular adhesion as well as signal transduction to control cellular proliferation. KEYWORDS: 3D-structure;Alternative splicing;Cell adhesion;Cell junction;Cell membrane;Cytoplasm;Cytoskeleton;Developmental protein;Differentiation;Membrane;Neurogenesis;Phosphoprotein;Reference proteome;Repeat;SH3 domain;Transducer SUBCELLULAR LOCATION: Cytoplasm Cell membrane ; Peripheral membrane protein ; Cytoplasmic side Basolateral cell membrane ; Peripheral membrane protein ; Cytoplasmic side Cytoplasm, cytoskeleton Cell junction, septate junction Note=Cytoskeleton- and membrane-associated. Located at the cytoplasmic face of the membrane in the cellular blastoderm and becomes associated with septate junctions which begin to form between epithelial cells at the time of dorsal closure. In adult flies, located at the apical-lateral membrane boundary of epithelial cells. MISCELLANEOUS: [Isoform B]: Contains the N-terminal domain essential for correct neuronal development.; MISCELLANEOUS: [Isoform F]: Contains the N-terminal domain essential for correct neuronal development.; MISCELLANEOUS: [Isoform H]: Contains the N-terminal domain essential for correct neuronal development.; MISCELLANEOUS: [Isoform I]: Contains the N-terminal domain essential for correct neuronal development.; MISCELLANEOUS: [Isoform L]: Contains the N-terminal domain essential for correct neuronal development." P31069,PROTEIN NAMES: Voltage-gated potassium channel Kch PROTEIN FAMILY: Potassium channel family ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the potassium channel family. FUNCTION: K(+)-specific ion channel. May play a role in the defense against osmotic shock. KEYWORDS: 3D-structure;Cell inner membrane;Cell membrane;Ion channel;Ion transport;Membrane;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell inner membrane ; Multi-pass membrane protein P31129,"PROTEIN NAMES: Diguanylate cyclase DgcZ (DGC) (Zinc-sensory diguanylate cyclase) ORGANISM: Escherichia coli (strain K12) FUNCTION: Catalyzes the synthesis of cyclic-di-GMP (c-di-GMP) via the condensation of 2 GTP molecules. May act as a zinc sensor that controls, via c-di-GMP, post-translational events. Overexpression leads to a strong repression of swimming; swimming returnes to normal when residues 206-207 are both mutated to Ala. Overexpression also leads to a reduction in flagellar abundance and a 20-fold increase in c-di-GMP levels in vivo. Required for aminoglycoside-mediated induction of biofilm formation, it also plays a lesser role in biofilm production in response to other classes of translation inhibitors. The c-di-GMP produced by this enzyme up-regulates poly-GlcNAc production as well as the biofilm synthesis protein PgaD, although c-di-GMP is probably not the main inducing principle. C-di-GMP is a second messenger which controls cell surface-associated traits in bacteria. PATHWAY: Purine metabolism; 3',5'-cyclic di-GMP biosynthesis. KEYWORDS: 3D-structure;Allosteric enzyme;GTP-binding;Magnesium;Metal-binding;Nucleotide-binding;Reference proteome;Transferase " P31178,"PROTEIN NAMES: Autolysin (Gamete lytic enzyme) (GLE) (Gametolysin) PROTEIN FAMILY: Peptidase M11 family ORGANISM: Chlamydomonas reinhardtii (Chlamydomonas smithii) SIMILARITY: Belongs to the peptidase M11 family. FUNCTION: Mediates digestion of the cell walls of the 2 mating type gametes during mating as a necessary prelude to cell fusion. This enzyme acts specifically on the framework proteins (inner wall) of the cell wall, cleaving several model peptides at specific sites. KEYWORDS: Cell wall;Cell wall biogenesis/degradation;Direct protein sequencing;Glycoprotein;Hydrolase;Metal-binding;Metalloprotease;Periplasm;Protease;Secreted;Signal;Zinc;Zymogen SUBCELLULAR LOCATION: Periplasm. Secreted, cell wall. Note=Stored in the periplasm of gametes until its release. Secreted concurrently with release of the cell walls. " P31291,"PROTEIN NAMES: Protein Wnt-8b (XWnt-8b) PROTEIN FAMILY: Wnt family ORGANISM: Xenopus laevis (African clawed frog) SIMILARITY: Belongs to the Wnt family. FUNCTION: Ligand for members of the frizzled family of seven transmembrane receptors. Plays a role in the initiation of dorsal axis development. May activate a Nieuwkoop center-like signaling pathway. KEYWORDS: Alternative splicing;Developmental protein;Disulfide bond;Extracellular matrix;Glycoprotein;Lipoprotein;Reference proteome;Secreted;Signal;Wnt signaling pathway SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix. " P31318,"PROTEIN NAMES: Protein arg11, mitochondrial [Cleaved into: N-acetyl-gamma-glutamyl-phosphate reductase (N-acetyl-glutamate semialdehyde dehydrogenase) (NAGSA dehydrogenase); Acetylglutamate kinase (N-acetyl-L-glutamate 5-phosphotransferase) (NAG kinase) (AGK)] PROTEIN FAMILY: Acetylglutamate kinase family; NAGSA dehydrogenase family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: In the N-terminal section; belongs to the acetylglutamate kinase family.; SIMILARITY: In the C-terminal section; belongs to the NAGSA dehydrogenase family. PATHWAY: Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 2/4.; PATHWAY: Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 3/4. KEYWORDS: Amino-acid biosynthesis;Arginine biosynthesis;ATP-binding;Cleavage on pair of basic residues;Kinase;Mitochondrion;Multifunctional enzyme;NADP;Nucleotide-binding;Oxidoreductase;Reference proteome;Transferase;Transit peptide SUBCELLULAR LOCATION: Mitochondrion " P31376,PROTEIN NAMES: SWR1-complex protein 3 PROTEIN FAMILY: SWC3 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the SWC3 family. FUNCTION: Component of the SWR1 complex which mediates the ATP-dependent exchange of histone H2A for the H2A variant HZT1 leading to transcriptional regulation of selected genes by chromatin remodeling. Involved in chromosome stability. KEYWORDS: Activator;Chromatin regulator;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: Present with 1380 molecules/cell in log phase SD medium. P31383,"PROTEIN NAMES: Protein phosphatase PP2A regulatory subunit A (PR65) PROTEIN FAMILY: Phosphatase 2A regulatory subunit A family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the phosphatase 2A regulatory subunit A family. FUNCTION: Phosphatase 2A affects a variety of biological processes in the cell such as transcription, cell cycle progression and cellular morphogenesis, and provides an initial identification of critical substrates for this phosphatase. The regulatory subunit may direct the catalytic subunit to distinct, albeit overlapping, subsets of substrates. KEYWORDS: Reference proteome;Repeat MISCELLANEOUS: Present with 16900 molecules/cell in log phase SD medium." P31385,"PROTEIN NAMES: Transcriptional regulatory protein DEP1 ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Component of the RPD3C(L) histone deacetylase complex (HDAC) responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. KEYWORDS: 3D-structure;Chromatin regulator;Cytoplasm;Nucleus;Phosphoprotein;Reference proteome;Repressor;Transcription;Transcription regulation SUBCELLULAR LOCATION: Cytoplasm. Nucleus. " P31466,"PROTEIN NAMES: Adenine permease AdeP PROTEIN FAMILY: Nucleobase:cation symporter-2 (NCS2) family, Azg-like subfamily ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) family. Azg-like subfamily. FUNCTION: High-affinity transporter for adenine. KEYWORDS: Cell inner membrane;Cell membrane;Membrane;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell inner membrane ; Multi-pass membrane protein " P31489,"PROTEIN NAMES: Adhesin YadA (Type 5 secretion system autotransporter YadA) PROTEIN FAMILY: Autotransporter-2 (AT-2) family ORGANISM: Yersinia enterocolitica SIMILARITY: Belongs to the autotransporter-2 (AT-2) (TC 1.B.40) family. FUNCTION: Collagen-binding outer membrane protein forming a fibrillar matrix on the bacterial cell surface. Promotes initial attachment and invasion of eukaryotic cells. Also protects the bacteria by being responsible for agglutination, serum resistance, complement inactivation and phagocytosis resistance. KEYWORDS: 3D-structure;Cell adhesion;Cell outer membrane;Coiled coil;Membrane;Plasmid;Protein transport;Signal;Transmembrane;Transmembrane beta strand;Transport;Virulence SUBCELLULAR LOCATION: Cell surface Cell outer membrane Note=The C-terminal translocator domain is localized in the outer membrane and the passenger domain is at the cell surface. " P31660,"PROTEIN NAMES: 2-methylcitrate synthase (2-MCS) (MCS) ((2S,3S)-2-methylcitrate synthase) (Citrate synthase) PROTEIN FAMILY: Citrate synthase family ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the citrate synthase family. FUNCTION: Involved in the catabolism of short chain fatty acids (SCFA) via the tricarboxylic acid (TCA)(acetyl degradation route) and via the 2-methylcitrate cycle I (propionate degradation route). Catalyzes the Claisen condensation of propionyl-CoA and oxaloacetate (OAA) to yield 2-methylcitrate (2-MC) and CoA. Also catalyzes the condensation of oxaloacetate with acetyl-CoA to yield citrate but with a lower specificity. PATHWAY: Organic acid metabolism; propanoate degradation.; PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 1/2. KEYWORDS: Direct protein sequencing;Reference proteome;Transferase;Tricarboxylic acid cycle " P31755,"PROTEIN NAMES: Initiation-specific alpha-1,6-mannosyltransferase (Outer chain elongation protein 1) PROTEIN FAMILY: Glycosyltransferase 32 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the glycosyltransferase 32 family. FUNCTION: Mannosyltransferase involved in outer chain elongation of asparagine-linked oligosaccharides of the type Man(9)GlcNAc(2). Adds the first alpha-1,6-mannose to the Man(8)GlcNAc(2) and Man(9)GlcNAc(2), but not Man(5)GlcNAc(2), endoplasmic reticulum intermediates. Represents the first enzymatic event required for synthesis of outer chain mannose linkages on yeast secretory proteins. Has also the potential to transfer a second alpha-1,6-mannose to the Man(8)GlcNAc(2) core oligosaccharide. KEYWORDS: Endoplasmic reticulum;Glycoprotein;Glycosyltransferase;Golgi apparatus;Membrane;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Single-pass type II membrane protein Golgi apparatus membrane ; Single-pass type II membrane protein Note=Is recycled between the trans-Golgi network and a late compartment of the endoplasmic reticulum. MISCELLANEOUS: Present with 9490 molecules/cell in log phase SD medium." P31896,"PROTEIN NAMES: Carbon monoxide dehydrogenase (CODH) PROTEIN FAMILY: Ni-containing carbon monoxide dehydrogenase family ORGANISM: Rhodospirillum rubrum SIMILARITY: Belongs to the Ni-containing carbon monoxide dehydrogenase family. FUNCTION: Allows growth in a CO-dependent manner in the dark. CODH oxidizes carbon monoxide coupled, via CooF, to the reduction of a hydrogen cation by a hydrogenase (possibly CooH). KEYWORDS: 3D-structure;4Fe-4S;Cell inner membrane;Cell membrane;Cytoplasm;Direct protein sequencing;Iron;Iron-sulfur;Membrane;Metal-binding;Nickel;Oxidoreductase SUBCELLULAR LOCATION: Cytoplasm. Cell inner membrane; Peripheral membrane protein; Cytoplasmic side. Note=Loosely attached to the inner membrane, probably via CooF. MISCELLANEOUS: Methyl viologen can act as acceptor. Inactivated by O(2)." P32019,"PROTEIN NAMES: Type II inositol 1,4,5-trisphosphate 5-phosphatase (75 kDa inositol polyphosphate-5-phosphatase) (Phosphoinositide 5-phosphatase) (5PTase) PROTEIN FAMILY: Inositol 1,4,5-trisphosphate 5-phosphatase type II family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the inositol 1,4,5-trisphosphate 5-phosphatase type II family. FUNCTION: Hydrolyzes phosphatidylinositol 4,5-bisphosphate (PtIns(4,5)P2) and the signaling molecule phosphatidylinositol 1,4,5-trisphosphate (PtIns(1,4,5)P3), and thereby modulates cellular signaling events. KEYWORDS: 3D-structure;Alternative splicing;Cytoplasm;Cytoplasmic vesicle;Direct protein sequencing;Endosome;Golgi apparatus;Hydrolase;Lipid metabolism;Lipoprotein;Magnesium;Membrane;Metal-binding;Methylation;Prenylation;Reference proteome SUBCELLULAR LOCATION: Cytoplasm, cytosol Endoplasmic reticulum-Golgi intermediate compartment Early endosome membrane Membrane ; Peripheral membrane protein ; Cytoplasmic side Cytoplasmic vesicle, phagosome membrane Golgi apparatus " P32021,"PROTEIN NAMES: 2-oxoglutarate-dependent ethylene/succinate-forming enzyme (EFE) (Ethylene-forming enzyme) (2-oxoglutarate dioxygenase (ethylene-forming)) (2-oxoglutarate/L-arginine monooxygenase/decarboxylase (succinate-forming)) PROTEIN FAMILY: Iron/ascorbate-dependent oxidoreductase family ORGANISM: Pseudomonas savastanoi pv. phaseolicola (Pseudomonas syringae pv. phaseolicola) SIMILARITY: Belongs to the iron/ascorbate-dependent oxidoreductase family. FUNCTION: Simultaneously catalyzes two reactions, namely formation of ethylene and of succinate from 2-oxoglutarate, with a molar ratio of 2:1. PATHWAY: Alkene biosynthesis; ethylene biosynthesis via 2-oxoglutarate. KEYWORDS: 3D-structure;Dioxygenase;Direct protein sequencing;Ethylene biosynthesis;Iron;Metal-binding;Oxidoreductase;Plasmid MISCELLANEOUS: A dual-circuit mechanism has been proposed in previous research for the complete reaction, in which the binding of L-arginine and 2-oxoglutarate in a Schiff-base structure generates a common intermediate for the two reactions." P32030,PROTEIN NAMES: Fork head domain transcription factor slp1 (Sloppy paired locus protein 1) ORGANISM: Drosophila melanogaster (Fruit fly) FUNCTION: Transcription factor involved in segmentation. Required for the formation of the mandibular lobe. Different levels of slp activity seem to be required in different segments. KEYWORDS: Developmental protein;DNA-binding;Nucleus;Pair-rule protein;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus. P32074,"PROTEIN NAMES: Coatomer subunit gamma (Gamma-coat protein) (Gamma-COP) PROTEIN FAMILY: COPG family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the COPG family. FUNCTION: The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. KEYWORDS: Cytoplasm;Cytoplasmic vesicle;Endosome;ER-Golgi transport;Golgi apparatus;Isopeptide bond;Membrane;Phosphoprotein;Protein transport;Reference proteome;Repeat;Transport;Ubl conjugation SUBCELLULAR LOCATION: Cytoplasm. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasmic vesicle, COPI-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Endosome. Note=The coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, as well as on the vesicles/buds originating from it. MISCELLANEOUS: Present with 77900 molecules/cell in log phase SD medium." P32143,PROTEIN NAMES: Sulfofructose kinase (SF kinase) PROTEIN FAMILY: Carbohydrate kinase PfkB family ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the carbohydrate kinase PfkB family. FUNCTION: Phosphorylates 6-deoxy-6-sulfo-D-fructose (SF) to 6-deoxy-6-sulfo-D-fructose 1-phosphate (SFP). Cannot phosphorylate fructose 6-phosphate. KEYWORDS: 3D-structure;ATP-binding;Carbohydrate metabolism;Kinase;Nucleotide-binding;Reference proteome;Transferase P32179,"PROTEIN NAMES: 3'(2'),5'-bisphosphate nucleotidase (3'(2'),5-bisphosphonucleoside 3'(2')-phosphohydrolase) (3'-phosphoadenosine-5'-phosphate phosphatase) (3'-phosphoadenosine-5'-phosphatase) (PAP phosphatase) (PAPase) (DPNPase) (Halotolerance protein HAL2) (Methionine-requiring protein 22) PROTEIN FAMILY: Inositol monophosphatase superfamily ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the inositol monophosphatase superfamily. FUNCTION: Phosphatase that converts adenosine 3'-phosphate 5'-phosphosulfate (PAPS) to adenosine 5'-phosphosulfate (APS) and 3'(2')-phosphoadenosine 5'-phosphate (PAP) to AMP. May regulate the flux of sulfur in the sulfur-activation pathway by converting PAPS to APS. Involved in salt tolerance. Confers resistance to lithium. Shows no activity on inositol mono- and diphosphates, 3'-AMP, AMP, nicotinamide adenine dinucleotide phosphate (NADP), and p-nitrophenylphosphate. KEYWORDS: 3D-structure;Cytoplasm;Hydrolase;Lithium;Magnesium;Metal-binding;Nucleus;Reference proteome;Stress response SUBCELLULAR LOCATION: Cytoplasm Nucleus MISCELLANEOUS: Present with 7330 molecules/cell in log phase SD medium." P32215,"PROTEIN NAMES: Pituitary adenylate cyclase-activating polypeptide type I receptor (PACAP type I receptor) (PACAP-R-1) (PACAP-R1) PROTEIN FAMILY: G-protein coupled receptor 2 family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the G-protein coupled receptor 2 family. FUNCTION: This is a receptor for PACAP-27 and PACAP-38. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. May regulate the release of adrenocorticotropin, luteinizing hormone, growth hormone, prolactin, epinephrine, and catecholamine. May play a role in spermatogenesis and sperm motility. Causes smooth muscle relaxation and secretion in the gastrointestinal tract. KEYWORDS: Alternative splicing;Cell membrane;Developmental protein;Differentiation;Disulfide bond;G-protein coupled receptor;Glycoprotein;Membrane;Phosphoprotein;Receptor;Reference proteome;Signal;Spermatogenesis;Transducer;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. " P32232,"PROTEIN NAMES: Cystathionine beta-synthase (Beta-thionase) (Hemoprotein H-450) (Serine sulfhydrase) PROTEIN FAMILY: Cysteine synthase/cystathionine beta-synthase family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the cysteine synthase/cystathionine beta-synthase family. FUNCTION: Hydro-lyase catalyzing the first step of the transsulfuration pathway, where the hydroxyl group of L-serine is displaced by L-homocysteine in a beta-replacement reaction to form L-cystathionine, the precursor of L-cysteine. This catabolic route allows the elimination of L-methionine and the toxic metabolite L-homocysteine (By similarity). Also involved in the production of hydrogen sulfide, a gasotransmitter with signaling and cytoprotective effects on neurons. PATHWAY: Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-homocysteine and L-serine: step 1/2. KEYWORDS: Alternative splicing;Amino-acid biosynthesis;CBS domain;Cysteine biosynthesis;Cytoplasm;Direct protein sequencing;Heme;Iron;Isopeptide bond;Lyase;Metal-binding;Nucleus;Phosphoprotein;Pyridoxal phosphate;Reference proteome;Ubl conjugation SUBCELLULAR LOCATION: Cytoplasm Nucleus " P32261,"PROTEIN NAMES: Antithrombin-III (ATIII) (Serpin C1) PROTEIN FAMILY: Serpin family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the serpin family. FUNCTION: Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin, matriptase-3/TMPRSS7, as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin (By similarity). KEYWORDS: Blood coagulation;Disulfide bond;Glycoprotein;Hemostasis;Heparin-binding;Phosphoprotein;Protease inhibitor;Reference proteome;Secreted;Serine protease inhibitor;Signal SUBCELLULAR LOCATION: Secreted, extracellular space " P32323,"PROTEIN NAMES: A-agglutinin anchorage subunit (A-agglutinin cell wall attachment subunit) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Cell wall anchoring subunit of the a-agglutinin heterodimer. S.cerevisiae a and alpha cells express the complementary cell surface glycoproteins a-agglutinin and alpha-agglutinin, respectively, which interact with one another to promote cellular aggregation during mating. KEYWORDS: Cell adhesion;Cell wall;Disulfide bond;Glycoprotein;GPI-anchor;Lipoprotein;Membrane;Pheromone response;Reference proteome;Repeat;Secreted;Signal SUBCELLULAR LOCATION: Secreted, cell wall Membrane ; Lipid-anchor, GPI-anchor Note=Covalently-linked GPI-modified cell wall protein (GPI-CWP). " P32325,"PROTEIN NAMES: DDK kinase regulatory subunit DBF4 (Dumbbell forming protein 4) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Regulatory subunit of the CDC7-DBF4 kinase, also called DBF4-dependent kinase (DDK), which is involved in cell cycle regulation of premitotic and premeiotic chromosome replication and in chromosome segregation. DDK plays an essential role in initiating DNA replication at replication origins by phosphorylating the MCM2 and MCM4 subunits of the MCM2-7 helicase complex. DBF4 recruits the catalytic subunit CDC7 to MCM2 and to origins of replication. DDK has also postreplicative functions in meiosis. DDK phosphorylates the meiosis-specific double-strand break protein MER2 for initiation of meiotic recombination. Interacts with CDC5 during meiosis to promote double-strand breaks and monopolar spindle orientation. Inhibits CDC5 activity during mitosis through direct binding to its PBD. KEYWORDS: 3D-structure;Cell cycle;Cell division;Chromosome partition;DNA replication;DNA-binding;Meiosis;Metal-binding;Mitosis;Phosphoprotein;Reference proteome;Zinc;Zinc-finger " P32336,"PROTEIN NAMES: Protein NUD1 ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Involved in astral microtubule organization by binding SCP72 to the outer plaque in a cell-cycle dependent manner. Required for the mitotic exit by facilitating the binding of TEMP1 to CDC15. Also involved in the pathway that organizes the shaping and sizing of the prospore membrane (PSM) during sporulation. KEYWORDS: Cell cycle;Cell division;Cytoplasm;Cytoskeleton;Isopeptide bond;Leucine-rich repeat;Meiosis;Mitosis;Nucleus;Phosphoprotein;Reference proteome;Repeat;Ubl conjugation SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body Nucleus envelope Note=Localizes to the meiotic outer plaque of the SPB, at the end of the meiotic spindles. MISCELLANEOUS: Present with 892 molecules/cell in log phase SD medium." P32339,PROTEIN NAMES: Heme-binding protein HMX1 ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Plays an important role in the degradation of heme under conditions of iron deprivation. KEYWORDS: Endoplasmic reticulum;Heme;Iron;Membrane;Metal-binding;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Single-pass type IV membrane protein P32350,"PROTEIN NAMES: Dual specificity protein kinase KNS1 PROTEIN FAMILY: Protein kinase superfamily, CMGC Ser/Thr protein kinase family, Lammer subfamily ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. Lammer subfamily. FUNCTION: Nonessential protein kinase. KEYWORDS: ATP-binding;Kinase;Nucleotide-binding;Phosphoprotein;Reference proteome;Serine/threonine-protein kinase;Transferase;Tyrosine-protein kinase " P32354,PROTEIN NAMES: Minichromosome maintenance protein 10 (Protein DNA43) PROTEIN FAMILY: MCM10 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the MCM10 family. FUNCTION: Required for DNA synthesis. Required for entry into or completion of S phase. Involved in DNA replication and seems to participate in the activation of the pre-replication complex (pre-RC) and in transcription elongation. May play a role as a key coordinator in assembling the replication fork. Proposed to function at replication origins following the binding of the MCM2-7 complex prior to the recruitment of CDC45. Probably is required to stimulate phosphorylation of the MCM2-7 complex by the CDC7-DBF4 kinase complex. May recruit the DNA polymerase alpha:primase complex to replication origins and is required to maintain it on chromatin independently of CDC45. May also play a role in transcriptional silencing. KEYWORDS: Cell cycle;DNA replication;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Ubl conjugation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Note=Colocalizes with ORC1 on chromatin independent from cell cycle. According to previous research is recruited to replication origins in a cell cycle regulated manner. MISCELLANEOUS: Present with 1860 molecules/cell in log phase SD medium. P32364,"PROTEIN NAMES: Kinesin-related protein SMY1 (Suppressor protein SMY1) PROTEIN FAMILY: TRAFAC class myosin-kinesin ATPase superfamily, Kinesin family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. FUNCTION: Possible microtubule-based motor that can interact or substitute with myosin 2 (MYO2). KEYWORDS: 3D-structure;ATP-binding;Cytoplasm;Cytoskeleton;Microtubule;Motor protein;Nucleotide-binding;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton MISCELLANEOUS: Present with 1920 molecules/cell in log phase SD medium." P32368,"PROTEIN NAMES: Phosphatidylinositol-3-phosphatase SAC1 (Phosphatidylinositol-4-phosphate phosphatase) (Recessive suppressor of secretory defect) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Phosphoinositide phosphatase which catalyzes the hydrolysis of phosphatidylinositol 3-phosphate (PtdIns(3)P) and phosphatidylinositol 4-phosphate (PtdIns(4)P). Has low activity towards phosphatidylinositol-3,5-bisphosphate (PtdIns(3,5)P2). May be involved in the coordination of the activities of the secretory pathway and the actin cytoskeleton. KEYWORDS: 3D-structure;Endoplasmic reticulum;Golgi apparatus;Hydrolase;Isopeptide bond;Membrane;Reference proteome;Transmembrane;Transmembrane helix;Ubl conjugation SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein Golgi apparatus membrane ; Multi-pass membrane protein MISCELLANEOUS: Present with 48000 molecules/cell in log phase SD medium." P32397,"PROTEIN NAMES: Coproporphyrinogen III oxidase PROTEIN FAMILY: Protoporphyrinogen/coproporphyrinogen oxidase family, Coproporphyrinogen III oxidase subfamily ORGANISM: Bacillus subtilis (strain 168) SIMILARITY: Belongs to the protoporphyrinogen/coproporphyrinogen oxidase family. Coproporphyrinogen III oxidase subfamily. FUNCTION: Involved in coproporphyrin-dependent heme b biosynthesis. Catalyzes the oxidation of coproporphyrinogen III to coproporphyrin III. Can also oxidize protoporphyrinogen IX to protoporphyrin-IX. The specific activity for the oxidation of coproporphyrinogen III is much higher than that for the oxidation of protoporphyrinogen IX. Can also oxidize mesoporphyrinogen IX, but not uroporphyrinogen III. PATHWAY: Porphyrin-containing compound metabolism; protoheme biosynthesis. KEYWORDS: 3D-structure;Cell membrane;Cytoplasm;Direct protein sequencing;FAD;Flavoprotein;Heme biosynthesis;Membrane;Oxidoreductase;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Cell membrane ; Peripheral membrane protein " P32419,"PROTEIN NAMES: Malate dehydrogenase, peroxisomal PROTEIN FAMILY: LDH/MDH superfamily, MDH type 1 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the LDH/MDH superfamily. MDH type 1 family. KEYWORDS: 3D-structure;Direct protein sequencing;Glyoxylate bypass;NAD;Oxidoreductase;Peroxisome;Reference proteome;Tricarboxylic acid cycle SUBCELLULAR LOCATION: Peroxisome. MISCELLANEOUS: Yeast contains at least 3 malate dehydrogenase isoenzymes: a mitochondrial (MDH1), a cytoplasmic (MDH2) and a peroxisomal (MDH3).; MISCELLANEOUS: Present with 3300 molecules/cell in log phase SD medium." P32432,"PROTEIN NAMES: Transcription factor SFP1 (Split finger protein 1) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Transcription factor that regulates ribosomal protein (RP) and ribosome biogenesis (Ribi) gene expression in response to nutrients and stress. Promotes RP gene expression under optimal growth conditions. Leaves the nucleus upon environmental challenges, resulting in a down-regulation of RP gene transcription. The effect of the environmental cues on SFP1 localization is mediated through the TOR pathway. Also regulates the expression of genes involved in the G2/M transition during the mitotic cell cycle and the DNA-damage response. Required for carbon-source modulation of cell size. KEYWORDS: Amyloid;Cytoplasm;DNA-binding;Metal-binding;Nucleus;Phosphoprotein;Prion;Reference proteome;Repeat;Stress response;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Nuclear under optimal growth conditions. Leaves the nucleus in response to stress or changes in nutrient availability. The [ISP+] aggregates appear to be nuclear. MISCELLANEOUS: [ISP+] is the prion form of SFP1. [ISP+] is the result of a conformational change of the cellular SFP1 protein that becomes self-propagating and infectious. This conformational change generates a form of SFP1 that assembles into amyloid fibrils. [ISP+]-aggregates accumulate in the nucleus, and results in significantly larger cell size and increased drug resistance. [ISP+] can be cured by GdnHCl. It is speculated that prion properties of transcription factors may generate an optimized phenotypic heterogeneity that buffers yeast populations against diverse environmental insults.; MISCELLANEOUS: Present with 259 molecules/cell in log phase SD medium." P32458,"PROTEIN NAMES: Cell division control protein 11 PROTEIN FAMILY: TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily, Septin GTPase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family. FUNCTION: Septins are GTPases involved in cytokinesis that assemble early in the cell cycle as a patch at the incipient bud site and form a ring approximate 15 minutes before bud emergence, which transforms into an hour-glass shaped collar of cortical filaments that spans both sides of the mother-bud neck. This collar persists until just before cytokinesis, when it splits into two rings that occupy opposite sides of the neck. The septins at the bud neck serve as a structural scaffold that recruits different components involved in diverse processes at specific stages during the cell cycle. Many proteins bind asymmetrically to the septin collar. The septin assembly is regulated by protein kinases GIN4 and/or CLA4. May act by recruiting MYO1 and HOF1, a protein involved in septation, to the site of cleavage. Septins are also involved in cell morphogenesis, bud site selection, chitin deposition, cell cycle regulation, cell compartmentalization and spore wall formation. CDCd11 with SHS1 11 are involved in the recruitment of BNI5 and thereby ensure efficient localization at the bud neck of MYO1, the type II myosin of the actomyosin contractile ring. KEYWORDS: 3D-structure;Acetylation;Cell cycle;Cell division;Coiled coil;GTP-binding;Isopeptide bond;Membrane;Nucleotide-binding;Phosphoprotein;Reference proteome;Ubl conjugation SUBCELLULAR LOCATION: Membrane Bud neck Note=Present at the bud neck during cell division. Interacts with phosphatidylinositol 4-phosphate and phosphatidylinositol 5-phosphate (PI(4)P and PI(5)P). MISCELLANEOUS: Present with 9280 molecules/cell in log phase SD medium." P32481,"PROTEIN NAMES: Eukaryotic translation initiation factor 2 subunit gamma (eIF2-gamma) PROTEIN FAMILY: TRAFAC class translation factor GTPase superfamily, Classic translation factor GTPase family, EIF2G subfamily ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EIF2G subfamily. FUNCTION: As a subunit of eukaryotic initiation factor 2 eIF2, involved in the early steps of protein synthesis. In the presence of GTP, eIF-2 forms a ternary complex with initiator tRNA Met-tRNAi and then recruits the 40S ribosomal complex and initiation factors eIF-1, eIF-1A and eIF-3 to form the 43S pre-initiation complex (43S PIC), a step that determines the rate of protein translation. The 43S PIC binds to mRNA and scans downstream to the initiation codon, where it forms a 48S initiation complex by codon-anticodon base pairing. This leads to the displacement of eIF-1 to allow GTPase-activating protein (GAP) eIF-5-mediated hydrolysis of eIF2-bound GTP. Hydrolysis of GTP and release of Pi, which makes GTP hydrolysis irreversible, causes the release of the eIF-2-GDP binary complex from the 40S subunit, an event that is essential for the subsequent joining of the 60S ribosomal subunit to form an elongation-competent 80S ribosome. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must be exchanged with GTP by way of a reaction catalyzed by GDP-GTP exchange factor (GEF) eIF-2B. KEYWORDS: 3D-structure;Cytoplasm;GTP-binding;Hydrolase;Initiation factor;Nucleotide-binding;Phosphoprotein;Protein biosynthesis;Reference proteome SUBCELLULAR LOCATION: Cytoplasm, cytosol MISCELLANEOUS: Present with 20800 molecules/cell in log phase SD medium." P32502,"PROTEIN NAMES: Translation initiation factor eIF2B subunit beta (GCD complex subunit GCD7) (Guanine nucleotide exchange factor subunit GCD7) (eIF2B GDP-GTP exchange factor subunit beta) PROTEIN FAMILY: EIF-2B alpha/beta/delta subunits family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the eIF-2B alpha/beta/delta subunits family. FUNCTION: Acts as a component of the translation initiation factor 2B (eIF2B) complex, which catalyzes the exchange of GDP for GTP on the eukaryotic initiation factor 2 (eIF2) complex gamma subunit. Its guanine nucleotide exchange factor activity is repressed when bound to eIF2 complex phosphorylated on the alpha subunit, thereby limiting the amount of methionyl-initiator methionine tRNA available to the ribosome and consequently global translation is repressed (By similarity). It activates the synthesis of GCN4 in yeast under amino acid starvation conditions by suppressing the inhibitory effects of multiple AUG codons present in the leader of GCN4 mRNA. It may promote either repression or activation of GCN4 expression depending on amino acid availability. GCD6 and GCD7 repress GCN4 expression at the translational level by ensuring that ribosomes which have translated UORF1 will reinitiate at UORF2, -3, or -4 and thus fail to reach the GCN4 start site. KEYWORDS: 3D-structure;Cytoplasm;Initiation factor;Protein biosynthesis;Reference proteome;Translation regulation SUBCELLULAR LOCATION: Cytoplasm, cytosol MISCELLANEOUS: Present with 6650 molecules/cell in log phase SD medium." P32504,"PROTEIN NAMES: Centromere DNA-binding protein complex CBF3 subunit A (Centromere-binding factor 2) (Chromosome transmission fidelity protein 14) (Kinetochore protein CTF14) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Acts as a component of the centromere DNA-binding protein complex CBF3, which is essential for chromosome segregation and movement of centromeres along microtubules. CBF3 is required for the recruitment of other kinetochore complexes to CEN DNA. It plays a role in the attachment of chromosomes to the spindle and binds selectively to a highly conserved DNA sequence called CDEIII, found in centromers and in several promoters. KEYWORDS: 3D-structure;Centromere;Chromosome;Cytoplasm;Cytoskeleton;Direct protein sequencing;DNA-binding;Nucleus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Nucleus Chromosome, centromere Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body Note=Spindle midzone during anaphase B. MISCELLANEOUS: Present with 1350 molecules/cell in log phase SD medium." P32523,"PROTEIN NAMES: Pre-mRNA-processing factor 19 (RING-type E3 ubiquitin transferase PRP19) PROTEIN FAMILY: WD repeat PRP19 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the WD repeat PRP19 family. FUNCTION: Probable ubiquitin-protein ligase involved in pre-mRNA splicing. Acts as a central component of the NTC complex (or PRP19-associated complex) that associates to the spliceosome to mediate conformational rearrangement or to stabilize the structure of the spliceosome after U4 snRNA dissociation, which leads to spliceosome maturation. It is also probably involved in DNA repair. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: 3D-structure;DNA damage;DNA repair;mRNA processing;mRNA splicing;Nucleus;Reference proteome;Repeat;Spliceosome;Transferase;Ubl conjugation pathway;WD repeat SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: Present with 11700 molecules/cell in log phase SD medium.; MISCELLANEOUS: The tetramer is an elongated particle consisting of four globular WD40 domains held together by a central stalk." P32524,"PROTEIN NAMES: Pre-mRNA-splicing factor PRP21 ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: mRNA splicing factors, PRP9, PRP11, and PRP21, are necessary for binding of the U2 snRNP to the pre-mRNA in an early step of spliceosome assembly. KEYWORDS: 3D-structure;mRNA processing;mRNA splicing;Nucleus;Reference proteome;Repeat;Spliceosome SUBCELLULAR LOCATION: Nucleus. MISCELLANEOUS: Present with 2490 molecules/cell in log phase SD medium." P32526,"PROTEIN NAMES: Karyogamy protein KAR9 (Cortical protein KAR9) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Involved in karyogamy. Component of a cortical adaptor complex that orients cytoplasmic microtubules. It may be involved in anchoring cytoplasmic microtubules to the cell cortex. KEYWORDS: 3D-structure;Cytoplasm;Cytoskeleton;Karyogamy;Microtubule;Nucleus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Nucleus. Cytoplasm, cytoskeleton. Note=Localizes to the tip of shmoo projections and to the tip of budding cells in a cell-cycle dependent manner. MISCELLANEOUS: Present with 656 molecules/cell in log phase SD medium." P32527,"PROTEIN NAMES: Zuotin (DnaJ-related protein ZUO1) (J protein ZUO1) (Heat shock protein 40 homolog ZUO1) (Ribosome-associated complex subunit ZUO1) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Component of the ribosome-associated complex (RAC), a heterodimeric chaperone complex involved in regulation of accurate translation termination and in folding or maintaining nascent polypeptides in a folding-competent state. RAC stimulates the ATPase activity of the ribosome-associated pool of Hsp70-type chaperones SSB1/SSB2 that bind to the nascent polypeptide chain. ZUO1 can act as a J-protein for SSB1/SSB2 only when associated with SSZ1. KEYWORDS: 3D-structure;Chaperone;Cytoplasm;Direct protein sequencing;DNA-binding;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cytoplasm MISCELLANEOUS: Present with 86400 molecules/cell in log phase SD medium." P32562,"PROTEIN NAMES: Cell cycle serine/threonine-protein kinase CDC5/MSD2 PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family, CDC5/Polo subfamily ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily. FUNCTION: Protein kinase required for the cell cycle where it is involved in mitotic exit. A component of the fear (CDC14 early anaphase release) network which promotes CDC14 release from the nucleolus during early anaphase. Phosphorylates SCC1/MCD1 and NET1. KEYWORDS: 3D-structure;ATP-binding;Cell cycle;Cell division;Kinase;Mitosis;Nucleotide-binding;Phosphoprotein;Reference proteome;Repeat;Serine/threonine-protein kinase;Transferase MISCELLANEOUS: Present with 1480 molecules/cell in log phase SD medium." P32569,"PROTEIN NAMES: Mediator of RNA polymerase II transcription subunit 17 (Mediator complex subunit 17) (Suppressor of RNA polymerase B 4) PROTEIN FAMILY: Mediator complex subunit 17 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the Mediator complex subunit 17 family. FUNCTION: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. The Mediator complex unfolds to an extended conformation and partially surrounds RNA polymerase II, specifically interacting with the unphosphorylated form of the C-terminal domain (CTD) of RNA polymerase II. The Mediator complex dissociates from the RNA polymerase II holoenzyme and stays at the promoter when transcriptional elongation begins. KEYWORDS: 3D-structure;Activator;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: Present with 1720 molecules/cell in log phase SD medium." P32573,"PROTEIN NAMES: Peroxisomal 2,4-dienoyl-CoA reductase SPS19 [(3E)-enoyl-CoA-producing] (Sporulation-specific protein SPX19) PROTEIN FAMILY: Short-chain dehydrogenases/reductases (SDR) family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FUNCTION: Auxiliary enzyme of beta-oxidation. Participates in the degradation of unsaturated fatty enoyl-CoA esters having double bonds in both even- and odd-numbered positions in peroxisome. Catalyzes the NADP-dependent reduction of 2,4-dienoyl-CoA to yield trans-3-enoyl-CoA. Dispensable for growth and sporulation on solid acetate and oleate media, but is essential for these processes to occur on petroselineate. KEYWORDS: Direct protein sequencing;Isopeptide bond;NADP;Oxidoreductase;Peroxisome;Reference proteome;Sporulation;Ubl conjugation SUBCELLULAR LOCATION: Peroxisome " P32579,"PROTEIN NAMES: Threonylcarbamoyl-AMP synthase (TC-AMP synthase) (L-threonylcarbamoyladenylate synthase) (Suppressor of upstream AUG protein 5) (t(6)A37 threonylcarbamoyladenosine biosynthesis protein SUA5) (tRNA threonylcarbamoyladenosine biosynthesis protein SUA5) PROTEIN FAMILY: SUA5 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the SUA5 family. FUNCTION: Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Likely catalyzes the conversion of L-threonine, HCO(3)(-)/CO(2) and ATP to give threonylcarbamoyl-AMP (TC-AMP) as the acyladenylate intermediate, with the release of diphosphate. Required for normal translation, by ensuring translation fidelity at the level of codon recognition, appropriate translation initiation selection and maintenance of reading frame. Also involved in telomere replication. Binds to single-stranded telomeric (ssTG) DNA and positively regulates telomere length. KEYWORDS: ATP-binding;Chromosome;Cytoplasm;DNA-binding;Nucleotide-binding;Nucleotidyltransferase;Nucleus;Reference proteome;Telomere;Transferase;Translation regulation;tRNA processing SUBCELLULAR LOCATION: Cytoplasm Nucleus Chromosome, telomere MISCELLANEOUS: Present with 538 molecules/cell in log phase SD medium." P32584,"PROTEIN NAMES: Protein-S-isoprenylcysteine O-methyltransferase (ICMT) (Isoprenylcysteine carboxylmethyltransferase) (Prenylated protein carboxyl methyltransferase) (PPMT) (Prenylcysteine carboxyl methyltransferase) (pcCMT) PROTEIN FAMILY: Class VI-like SAM-binding methyltransferase superfamily, Isoprenylcysteine carboxyl methyltransferase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the class VI-like SAM-binding methyltransferase superfamily. Isoprenylcysteine carboxyl methyltransferase family. FUNCTION: Mediates C-terminal methylation of the isoprenylated C-terminal cysteine in A-factor mating pheromone and Ras proteins. Does not have a preference for the farnesyl or geranylgeranyl moieties in the model substrates N-acetyl-S-farnesyl-L-cysteine (AFC) and N-acetyl-S-geranylgeranyl-L-cysteine (AGGC) in vitro. KEYWORDS: Endoplasmic reticulum;Membrane;Methyltransferase;Pheromone response;Reference proteome;S-adenosyl-L-methionine;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein MISCELLANEOUS: Present with 2690 molecules/cell in log phase SD medium." P32585,"PROTEIN NAMES: Mediator of RNA polymerase II transcription subunit 18 (Mediator complex subunit 18) (Suppressor of RNA polymerase B 5) PROTEIN FAMILY: Mediator complex subunit 18 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the Mediator complex subunit 18 family. FUNCTION: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. The Mediator complex unfolds to an extended conformation and partially surrounds RNA polymerase II, specifically interacting with the unphosphorylated form of the C-terminal domain (CTD) of RNA polymerase II. The Mediator complex dissociates from the RNA polymerase II holoenzyme and stays at the promoter when transcriptional elongation begins. KEYWORDS: 3D-structure;Activator;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: Present with 1011 molecules/cell in log phase SD medium." P32586,"PROTEIN NAMES: Tyrosine-protein phosphatase 2 (Protein-tyrosine phosphatase 2) (PTPase 2) PROTEIN FAMILY: Protein-tyrosine phosphatase family, Non-receptor class subfamily ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the protein-tyrosine phosphatase family. Non-receptor class subfamily. FUNCTION: Plays a role in inhibiting the onset of mitosis. Dephosphorylates sty1/spc1 and wis1/spc2/sty2. KEYWORDS: Cell cycle;Cell division;Cytoplasm;Hydrolase;Mitosis;Protein phosphatase;Reference proteome SUBCELLULAR LOCATION: Cytoplasm. " P32588,"PROTEIN NAMES: Nuclear and cytoplasmic polyadenylated RNA-binding protein PUB1 (ARS consensus-binding protein ACBP-60) (Poly uridylate-binding protein) (Poly(U)-binding protein) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: May be associated with hnRNA within the nucleus and remains associated during nucleocytoplasmic mRNA transport, once the proteins are in the cytoplasm, disassembly of PUB1-RNA complexes may occur prior to PAB1 binding and formation of a translationally competent RNP complex. Binds to polyadenylated RNA; prefers to bind poly(rU); binds to T-rich single-stranded DNA. KEYWORDS: 3D-structure;Acetylation;Cytoplasm;Direct protein sequencing;Methylation;Nucleus;Reference proteome;Repeat;RNA-binding SUBCELLULAR LOCATION: Cytoplasm Nucleus Cytoplasm, P-body Cytoplasm, Stress granule MISCELLANEOUS: Present with 49600 molecules/cell in log phase SD medium." P32599,"PROTEIN NAMES: Fimbrin (ABP67) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Binds to actin, and functionally associates with actin structures involved in the development and maintenance of cell polarity. KEYWORDS: Actin-binding;Calcium;Metal-binding;Reference proteome;Repeat MISCELLANEOUS: Present with 3510 molecules/cell in log phase SD medium." P32602,"PROTEIN NAMES: Alpha-soluble NSF attachment protein (SNAP-alpha) (N-ethylmaleimide-sensitive factor attachment protein alpha) (Vesicular-fusion protein SEC17) (alpha-SNAP chaperone) PROTEIN FAMILY: SNAP family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the SNAP family. FUNCTION: SNARE complex protein that binds to cis-SNARE complexes on membranes and is required for vesicular transport between the endoplasmic reticulum and the Golgi apparatus and for homotypic vacuole fusion. During the priming step of membrane fusion, is released from cis-SNARE complexes by SEC18 to establish a pool of unpaired SNAREs, which are required for interactions in trans during docking and fusion steps. Can displace HOPS from SNARE complexes, which may be a prerequisite for trans-SNARE complex disassembly and subsequent rounds of priming, docking and fusion. KEYWORDS: 3D-structure;Acetylation;Direct protein sequencing;ER-Golgi transport;Isopeptide bond;Membrane;Protein transport;Reference proteome;Transport;Ubl conjugation SUBCELLULAR LOCATION: Membrane; Peripheral membrane protein. " P32605,PROTEIN NAMES: U1 small nuclear ribonucleoprotein A (U1 snRNP A) (U1-A) (U1A) (Mutant U1 die protein 1) PROTEIN FAMILY: RRM U1 A/B'' family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the RRM U1 A/B'' family. FUNCTION: Involved in nuclear mRNA splicing. The principal role of the U1A is to help fold or maintain U1 RNA in an active configuration. It is the first snRNP to interact with pre-mRNA. This interaction is required for the subsequent binding of U2 snRNP and the U4/U6/U5 tri-snRNP. KEYWORDS: 3D-structure;mRNA processing;mRNA splicing;Nucleus;Reference proteome;Repeat;Ribonucleoprotein;RNA-binding;Spliceosome SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: Present with 2950 molecules/cell in log phase SD medium. P32623,"PROTEIN NAMES: Probable glycosidase CRH2 (Congo red hypersensitive protein 2) (Unknown transcript 2 protein) PROTEIN FAMILY: Glycosyl hydrolase 16 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the glycosyl hydrolase 16 family. FUNCTION: Probable glycosidase that plays a role in cell wall architecture. Required for the transfer of chitin to 1,6-beta-glucan in the cell wall. KEYWORDS: Cell wall;Cell wall biogenesis/degradation;Glycoprotein;GPI-anchor;Hydrolase;Lipoprotein;Membrane;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted, cell wall. Membrane; Lipid-anchor, GPI-anchor. Note=Covalently-linked GPI-modified cell wall protein (GPI-CWP), localized particularly in chitin-rich areas. Localizes to sites of polarized growth. Found at the incipient bud site, as a ring at the bud neck as the bud grows, and in the septum at the time of cytokinesis. Redistributes uniformly over the cell cortex upon heat stress. " P32641,"PROTEIN NAMES: Checkpoint protein RAD24 PROTEIN FAMILY: Rad17/RAD24 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the rad17/RAD24 family. FUNCTION: Participates in checkpoint pathways arrest of the cell cycle, a mechanism that allows the DNA repair pathways to act to restore the integrity of the DNA prior to DNA synthesis or separation of the replicated chromosomes. Regulates the DNA damage checkpoint pathway throughout the cell cycle, when associated with RCF5. Component of the RFC-like RAD24-RFC complex which loads the checkpoint clamp DDC1:MEC3:RAD17 complex and is involved in DNA repair pathways. During a clamp loading circle, the RFC:clamp complex binds to DNA and the recognition of the double-stranded/single-stranded junction stimulates ATP hydrolysis by RFC. The complex presumably provides bipartite ATP sites in which one subunit supplies a catalytic site for hydrolysis of ATP bound to the neighboring subunit. Dissociation of RFC from the clamp leaves the clamp encircling DNA. KEYWORDS: 3D-structure;ATP-binding;Cell cycle;DNA damage;Nucleotide-binding;Nucleus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: Present with 752 molecules/cell in log phase SD medium." P32695,"PROTEIN NAMES: tRNA-dihydrouridine(20/20a) synthase (U20-specific dihydrouridine synthase) (U20-specific Dus) (tRNA-dihydrouridine synthase A) PROTEIN FAMILY: Dus family, DusA subfamily ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the Dus family. DusA subfamily. FUNCTION: Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines. Specifically modifies U20 and U20a in tRNAs. KEYWORDS: Direct protein sequencing;Flavoprotein;FMN;NADP;Oxidoreductase;Reference proteome;RNA-binding;tRNA processing;tRNA-binding MISCELLANEOUS: DusB and DusC together account for about half of the 5,6-dihydrouridine modification observed in wild-type cellular tRNA, and DusA accounts for the other half. These three enzymes seem to act site-specifically on the tRNA D-loop and contain nonredundant catalytic functions in vivo." P32722,PROTEIN NAMES: Porin D (Imipenem/basic amino acid-specific outer membrane pore) (Outer membrane protein D2) PROTEIN FAMILY: Outer membrane porin (Opr) family ORGANISM: Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) SIMILARITY: Belongs to the outer membrane porin (Opr) (TC 1.B.25) family. FUNCTION: Porin with a specificity for basic amino acids. Also possesses serine protease activity. KEYWORDS: 3D-structure;Cell outer membrane;Direct protein sequencing;Hydrolase;Ion transport;Membrane;Porin;Protease;Reference proteome;Serine protease;Signal;Transmembrane;Transmembrane beta strand;Transport SUBCELLULAR LOCATION: Cell outer membrane ; Multi-pass membrane protein P32742,"PROTEIN NAMES: Inhibitor of nuclear factor kappa-B kinase epsilon subunit homolog 1 PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. FUNCTION: Serine/threonine-protein kinase, which plays a role in regulating allophagy, an autophagic process in which paternal organelles, including mitochondria and membranous organelles, are degraded in embryos. Phosphorylates the allophagy receptor allo-1, which is required for allophagy. KEYWORDS: Alternative splicing;ATP-binding;Autophagy;Cytoplasm;Kinase;Nucleotide-binding;Reference proteome;Serine/threonine-protein kinase;Transferase SUBCELLULAR LOCATION: Cytoplasm Note=Localizes to cytoplasmic puncta in oocytes. After fertilization, localizes to paternal organelles during meiosis I and meiosis II. Co-localizes with allo-1 in embryos and oocytes. " P32747,"PROTEIN NAMES: Dihydroorotate dehydrogenase (quinone), mitochondrial (DHOD) (DHODase) (DHOdehase) (Dihydroorotate oxidase) PROTEIN FAMILY: Dihydroorotate dehydrogenase family, Type 2 subfamily ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily. FUNCTION: In the de novo pyrimidine biosynthesis pathway, catalyzes the stereospecific oxidation of (S)-dihydroorotate to orotate with reduction of flavin and the transfer of electrons to ubiquinone, which is part of the respiratory chain. Does not use fumarate and NAD as electron acceptors. PATHWAY: Pyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (quinone route): step 1/1. KEYWORDS: Flavoprotein;FMN;Membrane;Mitochondrion;Mitochondrion inner membrane;Oxidoreductase;Phosphoprotein;Pyrimidine biosynthesis;Reference proteome;Transit peptide;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Mitochondrion inner membrane ; Single-pass membrane protein " P32756,PROTEIN NAMES: Choline O-acetyltransferase (CHOACTase) (ChAT) (Choline acetylase) PROTEIN FAMILY: Carnitine/choline acetyltransferase family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the carnitine/choline acetyltransferase family. FUNCTION: Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses (By similarity). Required in SIA sublateral cholinergic motor neurons for a left-right turning behavior that occurs during the lethargus phase of the normal sleep process called 'flipping'. During 'flipping' animals rotate 180 degrees about their longitudinal axis. KEYWORDS: Acyltransferase;Neurotransmitter biosynthesis;Reference proteome;Transferase P32783,"PROTEIN NAMES: mRNA cap guanine-N7 methyltransferase (mRNA (guanine-N(7))-methyltransferase) (mRNA cap methyltransferase) PROTEIN FAMILY: Class I-like SAM-binding methyltransferase superfamily, mRNA cap 0 methyltransferase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase superfamily. mRNA cap 0 methyltransferase family. FUNCTION: Responsible for methylating the 5'-cap structure of mRNAs. KEYWORDS: Methyltransferase;mRNA capping;mRNA processing;Nucleus;Reference proteome;RNA-binding;S-adenosyl-L-methionine;Transferase SUBCELLULAR LOCATION: Nucleus " P32784,"PROTEIN NAMES: Glycerol-3-phosphate O-acyltransferase 1 (G-3-P acyltransferase 1) (GPAT 1) (Dihydroxyacetone phosphate acyltransferase 1) (DHAP-AT 1) (Glycerol-3-phosphate / dihydroxyacetone phosphate acyltransferase 1) (Suppressor of choline-transport mutants 1) PROTEIN FAMILY: GPAT/DAPAT family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the GPAT/DAPAT family. FUNCTION: Dual substrate-specific glycerol-3-phosphate/dihydroxyacetone phosphate sn-1 acyltransferase, catalyzing the first and committed reaction in the de novo synthesis of glycerophospholipids and triacylglycerols (TAGs). Prefers Gly-3-P over dihydroxyacetone phosphate and has a marked preference for 16-carbon fatty acyl chains. Transfers a fatty acid from fatty acyl-CoA to the sn-1 position of glycerol-3-phosphate to produce lysophosphatidic acid (LysoPA). These lipids not only are precursors of glycerolipids, but also are dynamic components of signal transduction systems that control cell physiology. SCT1 is the primary supplier of diacylglycerols (DAG), used mainly in TAG synthesis and phosphatidylcholine (PC) synthesis through the CDP-choline pathway. Regulates fatty acid desaturation, that is, the ratio of unsaturated versus saturated fatty acyl chains, by competing with the desaturase OLE1 for the common substrate C16:0-CoA. Sequesters C16:0-CoA into lipids, thereby shielding it from desaturation by OLE1. PATHWAY: Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3. KEYWORDS: Acyltransferase;Endoplasmic reticulum;Lipid biosynthesis;Lipid metabolism;Membrane;Phospholipid biosynthesis;Phospholipid metabolism;Reference proteome;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein Note=Localizes to both perinuclear and cortical endoplasmic reticulum. MISCELLANEOUS: Present with 1050 molecules/cell in log phase SD medium." P32793,"PROTEIN NAMES: Protein YSC84 (LAS seventeen-binding protein 4) (LAS17-binding protein 4) PROTEIN FAMILY: SH3YL1 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the SH3YL1 family. FUNCTION: Essential for the organization of the actin cytoskeleton, fluid phase endocytosis and vesicle trafficking, together with LSB5. KEYWORDS: 3D-structure;Cytoplasm;Cytoskeleton;Phosphoprotein;Reference proteome;SH3 domain SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, actin patch Note=Cortical actin patches. " P32800,"PROTEIN NAMES: Peroxisomal biogenesis factor 2 (Peroxin-2) PROTEIN FAMILY: Pex2/pex10/pex12 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the pex2/pex10/pex12 family. FUNCTION: E3 ubiquitin-protein ligase component of a retrotranslocation channel required for peroxisome organization by mediating export of the PEX5 receptor from peroxisomes to the cytosol, thereby promoting PEX5 recycling. The retrotranslocation channel is composed of PEX2, PEX10 and PEX12; each subunit contributing transmembrane segments that coassemble into an open channel that specifically allows the passage of PEX5 through the peroxisomal membrane. PEX2 also regulates peroxisome organization by acting as a E3 ubiquitin-protein ligase. PEX2 ubiquitinates PEX5 during its passage through the retrotranslocation channel: catalyzes monoubiquitination of PEX5 at 'Cys-6', a modification that acts as a signal for PEX5 extraction into the cytosol. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Alternative initiation;Membrane;Metal-binding;Peroxisome;Protein transport;Reference proteome;Transferase;Transmembrane;Transmembrane helix;Transport;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Peroxisome membrane ; Multi-pass membrane protein MISCELLANEOUS: Present with 339 molecules/cell in log phase SD medium." P32833,PROTEIN NAMES: Origin recognition complex subunit 2 (Origin recognition complex 71 kDa subunit) PROTEIN FAMILY: ORC2 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the ORC2 family. FUNCTION: Component of the origin recognition complex (ORC) that binds origins of replication. It has a role in both chromosomal replication and mating type transcriptional silencing. Binds to the ARS consensus sequence (ACS) of origins of replication. KEYWORDS: 3D-structure;Direct protein sequencing;DNA replication;Nucleus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Nucleus. MISCELLANEOUS: Present with 1700 molecules/cell in log phase SD medium. P32844,PROTEIN NAMES: Exocyst complex component SEC6 PROTEIN FAMILY: SEC6 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the SEC6 family. FUNCTION: Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane. KEYWORDS: 3D-structure;Acetylation;Cytoplasm;Exocytosis;Protein transport;Reference proteome;Transport SUBCELLULAR LOCATION: Cytoplasm. MISCELLANEOUS: Present with 1080 molecules/cell in log phase SD medium. P32854,PROTEIN NAMES: Syntaxin PEP12 (Carboxypeptidase Y-deficient protein 12) (Vacuolar protein sorting-associated protein 6) (Vacuolar protein-targeting protein 13) PROTEIN FAMILY: Syntaxin family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the syntaxin family. FUNCTION: Plays a role in the sorting and targeting of vacuolar proteases. KEYWORDS: Coiled coil;Membrane;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix;Ubl conjugation SUBCELLULAR LOCATION: Membrane ; Single-pass type IV membrane protein P32860,"PROTEIN NAMES: NifU-like protein, mitochondrial PROTEIN FAMILY: NifU family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the NifU family. FUNCTION: Involved in iron homeostasis within the mitochondrion where it is involved in the assembly of iron-sulfur proteins. Together with BOL3, required during the last step of iron-sulfur protein assembly when the iron-sulfur cluster is inserted into the target protein. Required for protecting iron sulfur clusters from oxidative damage. KEYWORDS: 3D-structure;Mitochondrion;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Mitochondrion matrix MISCELLANEOUS: Present with 11300 molecules/cell in log phase SD medium." P32866,"PROTEIN NAMES: G protein-coupled receptor kinase 2 PROTEIN FAMILY: Protein kinase superfamily, AGC Ser/Thr protein kinase family, GPRK subfamily ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily. FUNCTION: Specifically phosphorylates the activated forms of G protein-coupled receptors (By similarity). Required during oogenesis and embryogenesis; component of a signaling pathway that functions during egg chamber maturation. KEYWORDS: ATP-binding;Developmental protein;Differentiation;Kinase;Membrane;Nucleotide-binding;Oogenesis;Phosphoprotein;Reference proteome;Repeat;Serine/threonine-protein kinase;Transferase SUBCELLULAR LOCATION: Membrane Note=Associated with nurse cell and oocyte plasma membranes during much of oogenesis. " P32895,"PROTEIN NAMES: Ribose-phosphate pyrophosphokinase 1 (Phosphoribosyl pyrophosphate synthase 1) PROTEIN FAMILY: Ribose-phosphate pyrophosphokinase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the ribose-phosphate pyrophosphokinase family. FUNCTION: 5-phosphoribose 1-diphosphate synthase involved in nucleotide, histidine, and tryptophan biosynthesis. Active in heteromultimeric complexes with other 5-phosphoribose 1-diphosphate synthases (PRS2, PRS3, PRS4 and PRS5). PATHWAY: Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1. KEYWORDS: ATP-binding;Cytoplasm;Kinase;Magnesium;Metal-binding;Nucleotide biosynthesis;Nucleotide-binding;Phosphoprotein;Reference proteome;Transferase SUBCELLULAR LOCATION: Cytoplasm MISCELLANEOUS: Present with 11700 molecules/cell in log phase SD medium." P32910,"PROTEIN NAMES: DNA-directed RNA polymerase III subunit RPC6 (RNA polymerase III subunit C6) (C34) (DNA-directed RNA polymerase III 36 kDa polypeptide) PROTEIN FAMILY: Eukaryotic RPC34/RPC39 RNA polymerase subunit family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the eukaryotic RPC34/RPC39 RNA polymerase subunit family. FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Involved in recruitment of Pol III to the preinitiation complex. Involved in the configuration of an initiation-competent form of RNA polymerase. KEYWORDS: 3D-structure;Direct protein sequencing;DNA-directed RNA polymerase;Nucleus;Reference proteome;Transcription SUBCELLULAR LOCATION: Nucleus. " P32913,PROTEIN NAMES: Vacuolar protein sorting-associated protein 17 (Carboxypeptidase Y-deficient protein 21) (Vacuolar protein targeting protein 3) PROTEIN FAMILY: VPS17 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the VPS17 family. FUNCTION: Component of the membrane-associated retromer complex which is essential in endosome-to-Golgi retrograde transport. The VPS5-VPS17 subcomplex may assemble onto the membrane to promote vesicle formation and is required for recycling the vacuolar protein-sorting receptor. Required for the sorting and delivery of a subset of soluble vacuolar hydrolases. Required for retention of late Golgi membrane proteins and vacuolar biogenesis. Involved in vacuolar fragmentation during hyperosmotic stress. KEYWORDS: 3D-structure;Coiled coil;Membrane;Phosphoprotein;Protein transport;Reference proteome;Transport SUBCELLULAR LOCATION: Endomembrane system ; Peripheral membrane protein ; Cytoplasmic side Note=Membrane-associated on the cytoplasmic side of either the Golgi complex or an intermediate in Golgi to vacuole transport. MISCELLANEOUS: Present with 7380 molecules/cell in log phase SD medium. P32916,"PROTEIN NAMES: Signal recognition particle receptor subunit alpha homolog (SR-alpha) (Docking protein alpha) (DP-alpha) PROTEIN FAMILY: GTP-binding SRP family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the GTP-binding SRP family. FUNCTION: Component of the SRP (signal recognition particle) receptor (SR). Ensures, in conjunction with the signal recognition particle, the correct targeting of the nascent secretory proteins to the endoplasmic reticulum membrane system. GTP hydrolysis may enhance the fidelity of and provide unidirectionality to the targeting reaction. It is important but not essential for cell growth. May be directly involved in mitochondrial protein import. KEYWORDS: 3D-structure;Endoplasmic reticulum;GTP-binding;Membrane;Nucleotide-binding;Phosphoprotein;Receptor;Reference proteome SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Peripheral membrane protein ; Cytoplasmic side Note=Thought to be anchored in the membrane through an interaction with SRP102/SR-beta, which contains a bona fide transmembrane domain. MISCELLANEOUS: Present with 2000 molecules/cell in log phase SD medium." P32921,"PROTEIN NAMES: Tryptophan--tRNA ligase, cytoplasmic (Tryptophanyl-tRNA synthetase) (TrpRS) [Cleaved into: T1-TrpRS; T2-TrpRS] PROTEIN FAMILY: Class-I aminoacyl-tRNA synthetase family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family. FUNCTION: Catalyzes the attachment of tryptophan to tRNA(Trp) in a two-step reaction: tryptophan is first activated by ATP to form Trp-AMP and then transferred to the acceptor end of the tRNA(Trp). Could also possess an angiostatic activity. KEYWORDS: Alternative splicing;Aminoacyl-tRNA synthetase;Angiogenesis;ATP-binding;Cytoplasm;Direct protein sequencing;Ligase;Nucleotide-binding;Phosphoprotein;Protein biosynthesis;Reference proteome SUBCELLULAR LOCATION: Cytoplasm " P32929,"PROTEIN NAMES: Cystathionine gamma-lyase (CGL) (CSE) (Cysteine desulfhydrase) (Cysteine-protein sulfhydrase) (Gamma-cystathionase) (Homocysteine desulfhydrase) PROTEIN FAMILY: Trans-sulfuration enzymes family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the trans-sulfuration enzymes family. FUNCTION: Catalyzes the last step in the trans-sulfuration pathway from L-methionine to L-cysteine in a pyridoxal-5'-phosphate (PLP)-dependent manner, which consists on cleaving the L,L-cystathionine molecule into L-cysteine, ammonia and 2-oxobutanoate. Part of the L-cysteine derived from the trans-sulfuration pathway is utilized for biosynthesis of the ubiquitous antioxidant glutathione. Besides its role in the conversion of L-cystathionine into L-cysteine, it utilizes L-cysteine and L-homocysteine as substrates (at much lower rates than L,L-cystathionine) to produce the endogenous gaseous signaling molecule hydrogen sulfide (H2S). In vitro, it converts two L-cysteine molecules into lanthionine and H2S, also two L-homocysteine molecules to homolanthionine and H2S, which can be particularly relevant under conditions of severe hyperhomocysteinemia (which is a risk factor for cardiovascular disease, diabetes, and Alzheimer's disease). Lanthionine and homolanthionine are structural homologs of L,L-cystathionine that differ by the absence or presence of an extra methylene group, respectively. Acts as a cysteine-protein sulfhydrase by mediating sulfhydration of target proteins: sulfhydration consists of converting -SH groups into -SSH on specific cysteine residues of target proteins such as GAPDH, PTPN1 and NF-kappa-B subunit RELA, thereby regulating their function. By generating the gasotransmitter H2S, it participates in a number of physiological processes such as vasodilation, bone protection, and inflammation (Probable). Plays an essential role in myogenesis by contributing to the biogenesis of H2S in skeletal muscle tissue (By similarity). Can also accept homoserine as substrate (By similarity). Catalyzes the elimination of selenocystathionine (which can be derived from the diet) to yield selenocysteine, ammonia and 2-oxobutanoate (By similarity). PATHWAY: Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-homocysteine and L-serine: step 2/2. KEYWORDS: 3D-structure;Alternative splicing;Amino-acid biosynthesis;Calmodulin-binding;Cysteine biosynthesis;Cytoplasm;Disease variant;Lipid metabolism;Lyase;Pyridoxal phosphate;Reference proteome SUBCELLULAR LOCATION: Cytoplasm. " P32943,"PROTEIN NAMES: S-phase entry cyclin-6 PROTEIN FAMILY: Cyclin family, Cyclin AB subfamily ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the cyclin family. Cyclin AB subfamily. FUNCTION: Involved in G1/S and or S phase progression. Interacts with CDC28. KEYWORDS: Cell cycle;Cell division;Cyclin;Reference proteome " P32944,"PROTEIN NAMES: Mitosis inhibitor protein kinase SWE1 (Wee1 homolog) PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family, WEE1 subfamily ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily. FUNCTION: Protein kinase that acts as a negative regulator of entry into mitosis (G2 to M transition) by phosphorylating and inhibiting the mitosis-promoting cyclin B-bound CDC28 at 'Tyr-19'. SWE1-mediated inhibition of CDC28 acts in a cell size or morphogenesis checkpoint to delay mitosis in response to defects in growth, actin organization or bud formation. Inhibits the activity of B-type cyclins in replication initiation strongly for CLB2, moderately for CLB3 and CLB4, and there is no apparent inhibition for CLB5 and CLB6, correlating with the normal expression timing of those cyclins. Hyperphosphorylation and degradation of SWE1 when all checkpoint requirement are met releases CLB2-CDC28 from inhibition and allows for progression through the cell cycle. SWE1-dependent CDC28 phosphorylation is also required for pachytene arrest upon activation of the recombination checkpoint during meiosis. Also involved in the regulation of nitrogen starvation- and short chain alcohol-induced filamentous growth, or filamentous differentiation in response to slowed DNA synthesis. Can act both on serines and on tyrosines. KEYWORDS: ATP-binding;Cell cycle;Cell division;Isopeptide bond;Kinase;Magnesium;Meiosis;Metal-binding;Mitosis;Nucleotide-binding;Nucleus;Phosphoprotein;Reference proteome;Serine/threonine-protein kinase;Transferase;Tyrosine-protein kinase;Ubl conjugation SUBCELLULAR LOCATION: Bud neck. Nucleus. Note=When SWE1 first accumulates in G1, it is localized to the nucleus. After bud emergence, a subpopulation is recruited to the daughter side of the mother-bud neck through HSL1 and its adapter HSL7, where it is susceptible to hyperphosphorylation and degradation. " P32953,"PROTEIN NAMES: (S)-mandelate dehydrogenase, mitochondrial (Flavocytochrome b) (L(+)-mandelate dehydrogenase) (L-MDH) PROTEIN FAMILY: Cytochrome b5 family; FMN-dependent alpha-hydroxy acid dehydrogenase family ORGANISM: Rhodotorula graminis (Yeast) SIMILARITY: In the N-terminal section; belongs to the cytochrome b5 family.; SIMILARITY: In the C-terminal section; belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family. FUNCTION: Catalyzes the oxidation of (S)-mandelate to benzoylformate and enables utilization of mandelate as substrate for growth. PATHWAY: Aromatic compound metabolism; (R)-mandelate degradation; benzoate from (R)-mandelate: step 2/4. KEYWORDS: Aromatic hydrocarbons catabolism;Direct protein sequencing;Electron transport;Flavoprotein;FMN;Heme;Iron;Mandelate pathway;Metal-binding;Mitochondrion;Oxidoreductase;Respiratory chain;Transit peptide;Transport SUBCELLULAR LOCATION: Mitochondrion intermembrane space " P33015,PROTEIN NAMES: Thiosulfate transporter TsuA (Thiosulfate uptake protein A) PROTEIN FAMILY: TsuA/YedE family ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the TsuA/YedE (TC 9.B.102) family. FUNCTION: Mediates thiosulfate uptake. KEYWORDS: Cell inner membrane;Cell membrane;Membrane;Reference proteome;Sulfate transport;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell inner membrane ; Multi-pass membrane protein P33160,"PROTEIN NAMES: Formate dehydrogenase (FDH) (NAD-dependent formate dehydrogenase) PROTEIN FAMILY: D-isomer specific 2-hydroxyacid dehydrogenase family, FDH subfamily ORGANISM: Pseudomonas sp. (strain 101) (Achromobacter parvulus T1) SIMILARITY: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. FDH subfamily. FUNCTION: Catalyzes the NAD(+)-dependent oxidation of formate to carbon dioxide. Formate oxidation is the final step in the methanol oxidation pathway in methylotrophic microorganisms. Has a role in the detoxification of exogenous formate in non-methylotrophic organisms. KEYWORDS: 3D-structure;Cytoplasm;Direct protein sequencing;NAD;Oxidoreductase SUBCELLULAR LOCATION: Cytoplasm " P33224,PROTEIN NAMES: Putative acyl-CoA dehydrogenase AidB PROTEIN FAMILY: Acyl-CoA dehydrogenase family ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the acyl-CoA dehydrogenase family. FUNCTION: Part of the adaptive DNA-repair response to alkylating agents. Could prevent alkylation damage by protecting DNA and destroying alkylating agents that have yet to reach their DNA target. Binds to double-stranded DNA with a preference for a DNA region that includes its own promoter. Shows weak isovaleryl-CoA dehydrogenase activity in vitro. KEYWORDS: 3D-structure;Cytoplasm;Direct protein sequencing;DNA-binding;FAD;Flavoprotein;Oxidoreductase;Reference proteome;Stress response SUBCELLULAR LOCATION: Cytoplasm P33299,PROTEIN NAMES: 26S proteasome regulatory subunit 7 homolog (Protein CIM5) (Tat-binding homolog 3) PROTEIN FAMILY: AAA ATPase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the AAA ATPase family. FUNCTION: The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex (By similarity). KEYWORDS: 3D-structure;ATP-binding;Cytoplasm;Nucleotide-binding;Nucleus;Phosphoprotein;Proteasome;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Nucleus MISCELLANEOUS: Present with 105 molecules/cell in log phase SD medium. P33308,"PROTEIN NAMES: Mediator of RNA polymerase II transcription subunit 9 (Chromosome segregation protein 2) (Mediator complex subunit 9) PROTEIN FAMILY: Mediator complex subunit 9 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the Mediator complex subunit 9 family. FUNCTION: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. The Mediator complex unfolds to an extended conformation and partially surrounds RNA polymerase II, specifically interacting with the unphosphorylated form of the C-terminal domain (CTD) of RNA polymerase II. The Mediator complex dissociates from the RNA polymerase II holoenzyme and stays at the promoter when transcriptional elongation begins. KEYWORDS: 3D-structure;Activator;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: Present with 1364 molecules/cell in log phase SD medium." P33330,"PROTEIN NAMES: Phosphoserine aminotransferase (PSAT) (Phosphohydroxythreonine aminotransferase) (Serine requiring protein 1) PROTEIN FAMILY: Class-V pyridoxal-phosphate-dependent aminotransferase family, SerC subfamily ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. SerC subfamily. FUNCTION: Phosphoserine aminotransferase (PSAT) is a pyridoxal 5'-phosphate-dependent enzyme involved in the second step of the phosphorylated pathway of serine biosynthesis. Catalyzes the reversible conversion of 3-phosphohydroxypyruvate to phosphoserine and of 3-hydroxy-2-oxo-4-phosphonooxybutanoate to phosphohydroxythreonine. Plays an indirect role in purine biosynthesis. PATHWAY: Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 2/3. KEYWORDS: 3D-structure;Amino-acid biosynthesis;Aminotransferase;Phosphoprotein;Pyridoxal phosphate;Reference proteome;Serine biosynthesis;Transferase MISCELLANEOUS: Present with 15900 molecules/cell in log phase SD medium." P33338,"PROTEIN NAMES: Protein SLA2 (Transmembrane protein MOP2) PROTEIN FAMILY: SLA2 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the SLA2 family. FUNCTION: Required for cellular morphogenesis and polarization of the cortical cytoskeleton. It might act in concert with proteins such as CDC42 and CDC43 to limit the region of cortical patch formation to the cortex of the bud. Required for the accumulation and/or maintenance of plasma membrane H(+)-ATPase on the cell surface. KEYWORDS: 3D-structure;Actin-binding;Cell membrane;Cytoplasm;Cytoskeleton;Membrane;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass membrane protein Cytoplasm, cytoskeleton, actin patch. Cytoplasm, cell cortex. Bud tip. MISCELLANEOUS: Present with 40600 molecules/cell in log phase SD medium." P33400,"PROTEIN NAMES: pH-response transcription factor pacC/RIM101 (Regulator of IME2 protein 1) (pH-response regulator protein RIM101) PROTEIN FAMILY: PacC/RIM101 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the pacC/RIM101 family. FUNCTION: Transcription factor that mediates regulation of both acid- and alkaline-expressed genes in response to ambient pH. At alkaline ambient pH, activates transcription of alkaline-expressed genes (including RIM101 itself), mainly by repressing transcriptional repressors of those genes, and represses transcription of acid-expressed genes. Required for meiosis, sporulation and invasive growth. KEYWORDS: Activator;Cytoplasm;DNA-binding;Meiosis;Metal-binding;Nucleus;Reference proteome;Repeat;Repressor;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cytoplasm Nucleus MISCELLANEOUS: Present with 1822 molecules/cell in log phase SD medium." P33402,PROTEIN NAMES: Guanylate cyclase soluble subunit alpha-2 (GCS-alpha-2) PROTEIN FAMILY: Adenylyl cyclase class-4/guanylyl cyclase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the adenylyl cyclase class-4/guanylyl cyclase family. FUNCTION: Has guanylyl cyclase on binding to the beta-1 subunit.; FUNCTION: Isoform 2 acts as a negative regulator of guanylyl cyclase activity as it forms non-functional heterodimers with the beta subunits. KEYWORDS: Alternative splicing;cGMP biosynthesis;Cytoplasm;GTP-binding;Lyase;Nucleotide-binding;Reference proteome SUBCELLULAR LOCATION: Cytoplasm. MISCELLANEOUS: There are two types of guanylate cyclases: soluble forms and membrane-associated receptor forms. P33413,PROTEIN NAMES: Urea active transporter PROTEIN FAMILY: Sodium:solute symporter (SSF) family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family. FUNCTION: Required for active transport of urea. KEYWORDS: Membrane;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. P33754,PROTEIN NAMES: Translocation protein SEC66 (Protein HSS1) (Sec62/63 complex 31.5 kDa subunit) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: To S.pombe SpBC409.21. FUNCTION: Acts as a component of the Sec62/63 complex which is involved in SRP-independent post-translational translocation across the endoplasmic reticulum (ER) and functions together with the Sec61 complex and KAR2 in a channel-forming translocon complex. A cycle of assembly and disassembly of Sec62/63 complex from SEC61 may govern the activity of the translocon. SEC66 is required to attach or retain SEC72 in the SEC63 complex. It is essential for growth at elevated temperatures. KEYWORDS: 3D-structure;Direct protein sequencing;Endoplasmic reticulum;Glycoprotein;Membrane;Protein transport;Reference proteome;Signal-anchor;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Single-pass type II membrane protein MISCELLANEOUS: Present with 7820 molecules/cell in log phase SD medium. P33767,"PROTEIN NAMES: Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit WBP1 (Oligosaccharyl transferase subunit WBP1) (Oligosaccharyl transferase subunit beta) PROTEIN FAMILY: DDOST 48 kDa subunit family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the DDOST 48 kDa subunit family. FUNCTION: Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity. PATHWAY: Protein modification; protein glycosylation. KEYWORDS: 3D-structure;Direct protein sequencing;Endoplasmic reticulum;Glycoprotein;Membrane;Reference proteome;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Single-pass type I membrane protein MISCELLANEOUS: Present with 14900 molecules/cell in log phase SD medium." P33896,"PROTEIN NAMES: Interferon alpha/beta receptor 1 (IFN-R-1) (IFN-alpha/beta receptor 1) (Type I interferon receptor 1) PROTEIN FAMILY: Type II cytokine receptor family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the type II cytokine receptor family. FUNCTION: Together with IFNAR2, forms the heterodimeric receptor for type I interferons (including interferons alpha, beta, epsilon, omega and kappa). Type I interferon binding activates the JAK-STAT signaling cascade, and triggers tyrosine phosphorylation of a number of proteins including JAKs, TYK2, STAT proteins and the IFNR alpha- and beta-subunits themselves (By similarity). STAT proteins are then phosphorylated by the JAKs, promoting their translocation into the nucleus to regulate expression of interferon-regulated genes (By similarity). Can also act independently of IFNAR2: form an active IFNB1 receptor by itself and activate a signaling cascade that does not involve activation of the JAK-STAT pathway. KEYWORDS: 3D-structure;Cell membrane;Disulfide bond;Endosome;Glycoprotein;Isopeptide bond;Lysosome;Membrane;Phosphoprotein;Receptor;Reference proteome;Repeat;Signal;Transmembrane;Transmembrane helix;Ubl conjugation SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein Late endosome Lysosome Note=Interferon binding triggers internalization of the receptor from the cell membrane into endosomes and then into lysosomes. MISCELLANEOUS: The interferon signaling pathway is not identical between species. Thus, the interaction with STAT1 and STAT2 may not be conserved in mouse; in human it requires phosphorylation at 'Tyr-466', but the mouse protein has a Phe at the equivalent position. Likewise, cysteine palmitoylation is required for the activation of STAT1 and STAT2 in human, but the Cys is not conserved in mouse." P34021,"PROTEIN NAMES: Ecdysone receptor (20-hydroxy-ecdysone receptor) (20E receptor) (EcRH) (Ecdysteroid receptor) (Nuclear receptor subfamily 1 group H member 1) PROTEIN FAMILY: Nuclear hormone receptor family, NR1 subfamily ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the nuclear hormone receptor family. NR1 subfamily. FUNCTION: Receptor for ecdysone. Binds to ecdysone response elements (ECRES) following ecdysone-binding, and recruitment of a complex containing the histone methyltransferase trr, leads to activate transcription of target genes. KEYWORDS: 3D-structure;Alternative splicing;DNA-binding;Metal-binding;Nucleus;Receptor;Reference proteome;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus " P34046,PROTEIN NAMES: Guanine nucleotide-binding protein alpha-8 subunit (G alpha-8) PROTEIN FAMILY: G-alpha family ORGANISM: Dictyostelium discoideum (Social amoeba) SIMILARITY: Belongs to the G-alpha family. FUNCTION: Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. G alpha-8 is a potential analog for the G(s)-like G-proteins which stimulate adenylate cyclase in mammals. KEYWORDS: GTP-binding;Lipoprotein;Magnesium;Metal-binding;Myristate;Nucleotide-binding;Palmitate;Reference proteome;Transducer P34082,"PROTEIN NAMES: Fasciclin-2 (Fasciclin II) (FAS II) ORGANISM: Drosophila melanogaster (Fruit fly) FUNCTION: Neuronal recognition molecule for the MP1 axon pathway, pathway recognition for axons during the development of nerve fascicles. KEYWORDS: Alternative splicing;Cell adhesion;Cell membrane;Developmental protein;Differentiation;Disulfide bond;Glycoprotein;GPI-anchor;Immunoglobulin domain;Lipoprotein;Membrane;Neurogenesis;Phosphoprotein;Reference proteome;Repeat;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: [Isoform 1]: Cell membrane ; Single-pass type I membrane protein.; SUBCELLULAR LOCATION: [Isoform 2]: Cell membrane ; Lipid-anchor, GPI-anchor MISCELLANEOUS: [Isoform 2]: GPI-anchored form." P34125,"PROTEIN NAMES: Diacylglycerol kinase A (Myosin heavy chain kinase) (MHCK) PROTEIN FAMILY: Eukaryotic diacylglycerol kinase family ORGANISM: Dictyostelium discoideum (Social amoeba) SIMILARITY: Belongs to the eukaryotic diacylglycerol kinase family. FUNCTION: Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids. Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes. Also plays an important role in the biosynthesis of complex lipids. PATHWAY: Lipid metabolism; glycerolipid metabolism. KEYWORDS: ATP-binding;Kinase;Lipid metabolism;Membrane;Metal-binding;Nucleotide-binding;Phosphoprotein;Reference proteome;Repeat;Serine/threonine-protein kinase;Transferase;Zinc;Zinc-finger SUBCELLULAR LOCATION: Membrane ; Peripheral membrane protein " P34167,PROTEIN NAMES: Eukaryotic translation initiation factor 4B (eIF-4B) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Involved in translation initiation. May be the homolog of mammalian eIF4B and be part of an RNA helicase. STM1/TIF3 is a non-essential gene. KEYWORDS: Initiation factor;Phosphoprotein;Protein biosynthesis;Reference proteome;Repeat;RNA-binding MISCELLANEOUS: Present with 24000 molecules/cell in log phase SD medium. P34219,"PROTEIN NAMES: Transcriptional regulatory protein TOD6 (PAC-binding factor 1) (Twin of DOT6) PROTEIN FAMILY: DOT6 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the DOT6 family. FUNCTION: Component of the RPD3 histone deacetylase complex RPD3C(L) responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. TOD6 binds to sequences containing the core CGATG, which resembles the PAC (Polymerase A and C) motif. KEYWORDS: Cytoplasm;DNA-binding;Nucleus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Nucleus MISCELLANEOUS: Present with 830 molecules/cell in log phase SD medium." P34223,PROTEIN NAMES: UBX domain-containing protein 1 (Suppressor of high-copy PP1 protein) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Involved in CDC48-dependent protein degradation through the ubiquitin/proteasome pathway. Direct or indirect positive regulator of GLC7 activity. KEYWORDS: 3D-structure;Cytoplasm;Isopeptide bond;Nucleus;Phosphoprotein;Reference proteome;Ubl conjugation;Ubl conjugation pathway SUBCELLULAR LOCATION: Nucleus Cytoplasm MISCELLANEOUS: Present with 3200 molecules/cell in log phase SD medium. P34233,"PROTEIN NAMES: Transcriptional regulatory protein ASH1 (Daughter cells HO repressor protein) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Component of the RPD3C(L) histone deacetylase complex (HDAC). Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. ASH1 is necessary to repress HO in daughter cells to block mating-type switching through its binding to HO promoter 5'-YTGAT-3' sites. Also involved in pseudohyphal growth. KEYWORDS: Chromatin regulator;DNA-binding;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Repressor;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Note=Preferentially accumulates in daughter cell nuclei at the end of anaphase. MISCELLANEOUS: The ASH1 mRNA is transported to the daughter cell before cytokinesis where translation produces the protein to block mating-type switching. The ASH1 mRNA 3'-UTR and the mRNA localization machinery that are essential to restrict accumulation to the bud.; MISCELLANEOUS: Present with 1800 molecules/cell in log phase SD medium." P34273,"PROTEIN NAMES: Iodotyrosine dehalogenase 1 homolog (IYD-1) PROTEIN FAMILY: Nitroreductase family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the nitroreductase family. FUNCTION: May contribute to coordination of muscle contraction as regulatory subunit of the nonessential sup-9 potassium channel complex. May act downstream of sup-10. KEYWORDS: Flavoprotein;FMN;Ion transport;Membrane;Oxidoreductase;Potassium;Potassium transport;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Membrane ; Single-pass membrane protein Note=In body-wall muscle cells, localizes to dense body-like structures which connect the myofibril lattice to the cell membrane. Colocalizes with sup-10. Membrane localization is not essential for its activity. " P34308,"PROTEIN NAMES: Calpain clp-1 PROTEIN FAMILY: Peptidase C2 family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the peptidase C2 family. FUNCTION: Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates (By similarity). Required for assembly and maintenance of integrin attachment complexes which are essential for maintenance of adult muscle. Proteolytic activity is activated in response to increased intracellular Ca(2+) levels during cell degeneration and promotes necrotic cell death. KEYWORDS: Alternative splicing;Cytoplasm;Hydrolase;Protease;Reference proteome;Thiol protease SUBCELLULAR LOCATION: Cytoplasm, myofibril, sarcomere, M line Cytoplasm, myofibril, sarcomere Note=In body wall muscle cells, localizes at M-lines extending over the H-zone, and at adhesion plaques which form between adjacent cells. " P34314,"PROTEIN NAMES: Serine/threonine-protein kinase tousled-like 1 (Tousled-like kinase 1) PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. FUNCTION: Essential for appropriate transcription during embryonic development. May act during transcription elongation to activate the RNA polymerase II large subunit (ama-1) by phosphorylating the Ser-2 residues of the C-terminal domain 7-residue repeats. Does not phosphorylate histone H3. KEYWORDS: Alternative splicing;ATP-binding;Developmental protein;Kinase;Nucleotide-binding;Nucleus;Phosphoprotein;Reference proteome;Serine/threonine-protein kinase;Transcription;Transcription regulation;Transferase SUBCELLULAR LOCATION: Nucleus " P34341,"PROTEIN NAMES: Condensin-2 complex subunit kle-2 (Kleisin, abnormal closure, protein 2) PROTEIN FAMILY: CND2 H2 (condensin-2 subunit 2) family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the CND2 H2 (condensin-2 subunit 2) family. FUNCTION: Regulatory subunit of the condensin II complex, a complex that seems to play a role in prophase chromosome condensation and in chromosome segregation in mitosis and in meiosis. KEYWORDS: Cell cycle;Cell division;Centromere;Chromosome;Coiled coil;Meiosis;Mitosis;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus Chromosome Chromosome, centromere Note=Localizes to the core of each sister chromatid in meiosis. " P34370,"PROTEIN NAMES: Inositol polyphosphate 5-phosphatase (Ciliary localization protein 1) PROTEIN FAMILY: Inositol 1,4,5-trisphosphate 5-phosphatase type II family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the inositol 1,4,5-trisphosphate 5-phosphatase type II family. FUNCTION: Dephosphorylates a number of phosphatidylinositols. Controls the cellular levels and subcellular distribution of phosphatidylinositol 3,5-bisphosphate and phosphatidylinositol 3,4,5-trisphosphate. Has a role in sperm activation and motility. Influences the localization of the transient receptor potential polycystin (TRPP) complex proteins lov-1 and pkd-2. KEYWORDS: Cytoplasm;Hydrolase;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Note=Observed in reticular structures. " P34400,"PROTEIN NAMES: Abnormal cell migration protein 10 PROTEIN FAMILY: MRL family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the MRL family. FUNCTION: Required cell non-autonomously for proper development of the excretory canals and for the long-range anterior-posterior migrations of embryonic neurons CAN, ALM and HSN. Plays a role, probably downstream of ced-10/rac1, in orientating axonal growth of HSN and AVM neurons in response to guidance cues such as slt-1. May regulate growth cone polarization by promoting asymmetric F-actin assembly. May be involved in signal transduction during cell migration. KEYWORDS: Alternative splicing;Developmental protein;Neurogenesis;Reference proteome SUBCELLULAR LOCATION: Perikaryon Note=Enriched at the ventral edge of HSN cell bodies. This asymmetric distribution is regulated by ced-10/rac1. " P34540,"PROTEIN NAMES: Kinesin heavy chain (Uncoordinated protein 116) (Protein unc-116) PROTEIN FAMILY: TRAFAC class myosin-kinesin ATPase superfamily, Kinesin family, Kinesin subfamily ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. Kinesin subfamily. FUNCTION: Microtubule-dependent motor protein required for organelle transport. Plays a role in endosome transport. Required for the transport of mitochondria along the axon of motor neurons. Involved in the nuclear migration of hyp7 hypodermal precursor cells. Required for the formation of dendritic branches of PVD sensory neurons. In non-ciliated neurons such as the PVD and PHC neurons, required for the organization of minus-end out microtubules in dendrites. Also required for the minus-end out orientation of microtubules in dendrites of AQR gas-sensing neurons. Involved in the localization of unc-33 to neurites. Positively regulates cilium position and dendrite morphogenesis in the postembryonic AQR and PQR gas-sensing neurons. Plays a more prominent role in regulating dendrite morphogenesis in AQR than in PQR neurons. Plays a role in regulating the localization of grdn-1 to the distal dendrites of AQR sensory neurons. KEYWORDS: ATP-binding;Coiled coil;Cytoplasm;Cytoskeleton;Microtubule;Motor protein;Nucleotide-binding;Reference proteome;Transport SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton Cytoplasm " P34547,"PROTEIN NAMES: Ubiquitin carboxyl-terminal hydrolase 46 (Deubiquitinating enzyme) (Ubiquitin thioesterase) (Ubiquitin-specific-processing protease) PROTEIN FAMILY: Peptidase C19 family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the peptidase C19 family. FUNCTION: Regulates the abundance of the glr-1 glutamate receptor in the ventral nerve cord by promoting its deubiquitination and preventing its degradation in the lysosome. Contributes to the regulation of embryonic polarity. KEYWORDS: Alternative splicing;Cytoplasm;Hydrolase;Lipoprotein;Myristate;Protease;Reference proteome;Thiol protease;Ubl conjugation pathway SUBCELLULAR LOCATION: Perikaryon Cytoplasm Note=In ventral nerve cord interneurons, localized to discrete puncta within the perikaryon. In the ventral nerve cord, localized diffusely throughout the cytoplasm and to punctate structures. " P34644,"PROTEIN NAMES: Probable vesicular glutamate transporter eat-4 (Abnormal pharyngeal pumping eat-4) PROTEIN FAMILY: Major facilitator superfamily, Sodium/anion cotransporter family, VGLUT subfamily ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the major facilitator superfamily. Sodium/anion cotransporter family. VGLUT subfamily. FUNCTION: Required for glutamatergic synaptic transmission. In AWB and AWC sensory neurons, required for the detection of preferred food sources, probably via glutamatergic neurotransmission from sensory neurons. Negatively regulates the turning step of male mating behavior. KEYWORDS: Cell membrane;Glycoprotein;Ion transport;Membrane;Neurotransmitter transport;Reference proteome;Sodium;Sodium transport;Symport;Synapse;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Synapse. " P34684,"PROTEIN NAMES: Homeobox protein lin-39 (Abnormal cell lineage protein 39) PROTEIN FAMILY: Antp homeobox family, Deformed subfamily ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the Antp homeobox family. Deformed subfamily. FUNCTION: Transcription factor. Binds to the consensus 5'-TGATNNAT(G/T)(G/A)-3' PBC/Hox motif of target genes to regulate gene expression; sem-2 in order to promote cell fate specification in the postembryonic mesoderm (also known as the M lineage), and egl-18 and elt-6 to regulate vulval development. May bind to the PBC/Hox motif as a heterodimer with homeobox protein ceh-20. Regulates vulval precursor cell (VPC) differentiation, in concert with orphan nuclear receptor nhr-25. Regulates the expression of mig-13, which controls the asymmetric distribution of actin cytoskeleton-binding protein cor-1 in Q neuroblasts. This in turn controls the polarity of migration of Q neuroblasts and the subsequent mid-body region-specific development. KEYWORDS: Developmental protein;DNA-binding;Homeobox;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " P34722,"PROTEIN NAMES: Protein kinase C-like 1 (PKC) (Tetradecanoyl phorbol acetate-resistant protein 1) PROTEIN FAMILY: Protein kinase superfamily, AGC Ser/Thr protein kinase family, PKC subfamily ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. FUNCTION: Diacylglycerol (DAG)-dependent serine/threonine-protein kinase that phosphorylates a range of cellular proteins (Probable). Phosphorylates mlk-1, a component of the JNK pathway. Involved in axon regeneration after injury probably by activating the JNK pathway. Plays a role in resistance to fungal infection and in wound healing by promoting expression of antimicrobial peptide nlp-29 in the epidermis downstream of gpa-12 and plc-3 and upstream of tir-1-p38-like pathway. Probably by regulating neuronal transmission in ALA neurons, regulates the decrease in pharyngeal pumping during the quiescent state that precedes each larval molt, downstream of lin-3 and receptor let-23 and phospholipase plc-3. KEYWORDS: Alternative splicing;ATP-binding;Kinase;Metal-binding;Nucleotide-binding;Phosphoprotein;Reference proteome;Repeat;Serine/threonine-protein kinase;Transferase;Zinc;Zinc-finger " P34761,PROTEIN NAMES: Protein WHI3 ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Involved in size control and cell cycle. KEYWORDS: Cell cycle;Phosphoprotein;Reference proteome;RNA-binding MISCELLANEOUS: Present with 5730 molecules/cell in log phase SD medium. P34791,"PROTEIN NAMES: Peptidyl-prolyl cis-trans isomerase CYP20-3, chloroplastic (PPIase CYP20-3) (Cyclophilin of 20 kDa 3) (Cyclosporin A-binding protein) (Rotamase CYP20-3) (Rotamase cyclophilin-4) PROTEIN FAMILY: Cyclophilin-type PPIase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the cyclophilin-type PPIase family. FUNCTION: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Required for the light-induced increase of thiol accumulation. Assists the folding or assembly of SAT1 enzyme to form the cysteine synthase complex. Links light and redox signals to the regulation of cysteine biosynthesis in response to stress. KEYWORDS: Alternative splicing;Chaperone;Chloroplast;Direct protein sequencing;Disulfide bond;Isomerase;Plastid;Reference proteome;Rotamase;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast stroma Note=Probably associated to membranes. " P34889,"PROTEIN NAMES: Protein Wnt-2 PROTEIN FAMILY: Wnt family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the Wnt family. FUNCTION: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. Involved in the correct positioning of the developing nerve ring and in axon guidance of SIA and SIB neurons, probably by binding to tyrosine kinase receptor cam-1. In addition, regulates the positioning of some head neuronal cells, muscle arms associated with the nerve ring and the excretory pore. Together with Wnt ligand cwn-1, regulates the migration of CAN, ALM, BDU and HSN neurons during embryogenesis, the migration of QL and QR neuroblast descendants during larval development, and polarity of ALM neurons. May act through the wnt receptor cfz-2 to regulate QR neuroblast descendant migration, and to direct ALM migration. Also plays a role in axon growth and guidance in HSN and male CP neurons. In addition, together with wnt ligand cwn-1, negatively regulates developmental neurite pruning of AIM neurons probably by acting as a ligand for receptor tyrosine kinase cam-1. Through the cam-1 receptor also probably regulates the outgrowth of neurites from RME GABAergic motor neurons. May act redundantly with other Wnt ligands such as cwn-1 and mom-2 to control seam cell polarity. KEYWORDS: Developmental protein;Disulfide bond;Extracellular matrix;Glycoprotein;Lipoprotein;Neurogenesis;Reference proteome;Secreted;Signal;Wnt signaling pathway SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix " P34909,"PROTEIN NAMES: General negative regulator of transcription subunit 4 (Modulator of transcription 2) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: E3 ubiquitin-protein ligase component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover. The NOT protein subcomplex negatively regulates the basal and activated transcription of many genes. Preferentially affects TC-type TATA element-dependent transcription. Could directly or indirectly inhibit component(s) of the general transcription machinery. In the cytoplasm, catalyzes monoubiquitination of RPS7/es7 in response to stalled ribosomes, initiating a HEL2-dependent response that activates the No-Go Decay (NGD) pathway. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: 3D-structure;Activator;Coiled coil;Cytoplasm;Isopeptide bond;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Repressor;RNA-binding;Transcription;Transcription regulation;Transferase;Ubl conjugation;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cytoplasm Nucleus. MISCELLANEOUS: Present with 4280 molecules/cell in log phase SD medium." P34913,"PROTEIN NAMES: Bifunctional epoxide hydrolase 2 [Includes: Cytosolic epoxide hydrolase 2 (CEH) (Epoxide hydratase) (Soluble epoxide hydrolase) (SEH); Lipid-phosphate phosphatase ] PROTEIN FAMILY: AB hydrolase superfamily, Epoxide hydrolase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the AB hydrolase superfamily. Epoxide hydrolase family. FUNCTION: Bifunctional enzyme. The C-terminal domain has epoxide hydrolase activity and acts on epoxides (alkene oxides, oxiranes) and arene oxides. Plays a role in xenobiotic metabolism by degrading potentially toxic epoxides (By similarity). Also determines steady-state levels of physiological mediators.; FUNCTION: Bifunctional enzyme. The N-terminal domain has lipid phosphatase activity, with the highest activity towards threo-9,10-phosphonooxy-hydroxy-octadecanoic acid, followed by erythro-9,10-phosphonooxy-hydroxy-octadecanoic acid, 12-phosphonooxy-octadec-9Z-enoic acid and 12-phosphonooxy-octadec-9E-enoic acid. Has phosphatase activity toward lyso-glycerophospholipids with also some lower activity toward lysolipids of sphingolipid and isoprenoid phosphates. KEYWORDS: 3D-structure;Acetylation;Alternative splicing;Aromatic hydrocarbons catabolism;Cytoplasm;Detoxification;Direct protein sequencing;Hydrolase;Lipid metabolism;Lipoprotein;Magnesium;Metal-binding;Multifunctional enzyme;Peroxisome;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cytoplasm. Peroxisome. " P34925,"PROTEIN NAMES: Tyrosine-protein kinase RYK PROTEIN FAMILY: Protein kinase superfamily, Tyr protein kinase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. FUNCTION: May be a coreceptor along with FZD8 of Wnt proteins, such as WNT1, WNT3, WNT3A and WNT5A. Involved in neuron differentiation, axon guidance, corpus callosum establishment and neurite outgrowth. In response to WNT3 stimulation, receptor C-terminal cleavage occurs in its transmembrane region and allows the C-terminal intracellular product to translocate from the cytoplasm to the nucleus where it plays a crucial role in neuronal development. KEYWORDS: 3D-structure;Alternative splicing;ATP-binding;Cytoplasm;Disulfide bond;Glycoprotein;Kinase;Membrane;Nucleotide-binding;Nucleus;Phosphoprotein;Receptor;Reference proteome;Signal;Transferase;Transmembrane;Transmembrane helix;Tyrosine-protein kinase;Wnt signaling pathway SUBCELLULAR LOCATION: Membrane ; Single-pass type I membrane protein Nucleus Cytoplasm Note=In cells that have undergone neuronal differentiation, the C-terminal cleaved part is translocated from the cytoplasm to the nucleus. " P34969,"PROTEIN NAMES: 5-hydroxytryptamine receptor 7 (5-HT-7) (5-HT7) (5-HT-X) (Serotonin receptor 7) PROTEIN FAMILY: G-protein coupled receptor 1 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the G-protein coupled receptor 1 family. FUNCTION: This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that stimulate adenylate cyclase. KEYWORDS: 3D-structure;Alternative splicing;Cell membrane;Disulfide bond;G-protein coupled receptor;Glycoprotein;Lipoprotein;Membrane;Palmitate;Receptor;Reference proteome;Transducer;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. " P34972,"PROTEIN NAMES: Cannabinoid receptor 2 (CB-2) (CB2) (hCB2) (CX5) PROTEIN FAMILY: G-protein coupled receptor 1 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the G-protein coupled receptor 1 family. FUNCTION: Heterotrimeric G protein-coupled receptor for endocannabinoid 2-arachidonoylglycerol mediating inhibition of adenylate cyclase. May function in inflammatory response, nociceptive transmission and bone homeostasis. KEYWORDS: 3D-structure;Cell membrane;Cell projection;G-protein coupled receptor;Glycoprotein;Inflammatory response;Membrane;Phosphoprotein;Receptor;Reference proteome;Transducer;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Cell projection, dendrite Perikaryon Note=Localizes to apical dendrite of pyramidal neurons. " P34998,"PROTEIN NAMES: Corticotropin-releasing factor receptor 1 (CRF-R-1) (CRF-R1) (CRFR-1) (Corticotropin-releasing hormone receptor 1) (CRH-R-1) (CRH-R1) PROTEIN FAMILY: G-protein coupled receptor 2 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the G-protein coupled receptor 2 family. FUNCTION: G-protein coupled receptor for CRH (corticotropin-releasing factor) and UCN (urocortin). Has high affinity for CRH and UCN. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and down-stream effectors, such as adenylate cyclase. Promotes the activation of adenylate cyclase, leading to increased intracellular cAMP levels. Inhibits the activity of the calcium channel CACNA1H. Required for normal embryonic development of the adrenal gland and for normal hormonal responses to stress. Plays a role in the response to anxiogenic stimuli. KEYWORDS: 3D-structure;Alternative splicing;Cell membrane;Direct protein sequencing;Disulfide bond;Endosome;G-protein coupled receptor;Glycoprotein;Membrane;Phosphoprotein;Receptor;Reference proteome;Signal;Transducer;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Endosome. Note=Agonist-binding promotes endocytosis. MISCELLANEOUS: [Isoform CRF-R2]: Major isoform.; MISCELLANEOUS: [Isoform CRF-R3]: Does not bind to CRF with high affinity." P35056,"PROTEIN NAMES: Peroxisomal targeting signal receptor (PTS1 receptor) (PTS1R) (Peroxin-5) PROTEIN FAMILY: Peroxisomal targeting signal receptor family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the peroxisomal targeting signal receptor family. FUNCTION: Receptor that mediates peroxisomal import of proteins containing a C-terminal PTS1-type tripeptide peroxisomal targeting signal (SKL-type). Binds to cargo proteins containing a PTS1 peroxisomal targeting signal in the cytosol, and translocates them into the peroxisome matrix by passing through the PEX13-PEX14 docking complex along with cargo proteins. PEX5 receptor is then retrotranslocated into the cytosol, leading to release of bound cargo in the peroxisome matrix, and reset for a subsequent peroxisome import cycle. KEYWORDS: Acetylation;Cytoplasm;Isopeptide bond;Peroxisome;Phosphoprotein;Protein transport;Reference proteome;Repeat;Thioester bond;TPR repeat;Translocation;Transport;Ubl conjugation SUBCELLULAR LOCATION: Cytoplasm, cytosol Peroxisome matrix Note=Cycles between the cytosol and the peroxisome matrix. Following binding to cargo proteins containing a PTS1 peroxisomal targeting signal in the cytosol, recruited to the docking complex, composed of PEX13 and PEX14, leading to translocation into the peroxisome matrix along with cargo proteins. Export and recycling to the cytosol is initiated by binding to the PEX2-PEX10-PEX12 ligase complex via its unstructured N-terminus that inserts into the ligase pore and emerges in the cytosol. Cys-6 of PEX5 is then monoubiquitinated, promoting its extraction from peroxisomal membrane by the PEX1-PEX6 AAA ATPase complex. Extraction is accompanied by unfolding of the TPR repeats and release of bound cargo in the peroxisome matrix (By similarity). The TPR repeats refold in the cytosol and ubiquitination is removed by deubiquitinating enzyme UBP15, resetting PEX5 for a subsequent import cycle. MISCELLANEOUS: Present with 2070 molecules/cell in log phase SD medium." P35189,"PROTEIN NAMES: Transcription initiation factor TFIID subunit 14 (Actin non-complementing mutant 1) (Chromosome stability protein 10) (SWI/SNF chromatin-remodeling complex subunit TAF14) (SWI/SNF complex 29 kDa subunit) (SWI/SNF complex subunit TAF14) (TBP-associated factor 14) (TBP-associated factor 30 kDa) (Transcription factor G 30 kDa subunit) (Transcription initiation factor TFIIF 30 kDa subunit) PROTEIN FAMILY: TAF14 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the TAF14 family. FUNCTION: Functions as a component of the DNA-binding general transcription factor complex TFIID, the RNA polymerase II associated general transcription factor complex TFIIF, and the chromatin-remodeling complex SWI/SNF. Binding of TFIID to a promoter (with or without TATA element) is the initial step in preinitiation complex (PIC) formation. TFIID plays a key role in the regulation of gene expression by RNA polymerase II through different activities such as transcription activator interaction, core promoter recognition and selectivity, TFIIA and TFIIB interaction, chromatin modification (histone acetylation by TAF1), facilitation of DNA opening and initiation of transcription. TFIIF is essential for the initiation of transcription by RNA polymerase II. TFIIF functions include the recruitment of RNA polymerase II to the promoter bound DNA-TBP-TFIIB complex, decreasing the affinity of RNA polymerase II for non-specific DNA, allowing for the subsequent recruitment of TFIIE and TFIIH, and facilitating RNA polymerase II elongation. TAF14 acts as a chromatin reader that specifically recognizes and binds histones that are acylated. Recognizes and binds histone H3 acetylated or crotonylated at 'Lys-9' (H3K9ac and H3K9cr, respectively), with some preference for crotonylated lysine. Component of the SWI/SNF complex, an ATP-dependent chromatin-remodeling complex, is required for the positive and negative regulation of gene expression of a large number of genes. It changes chromatin structure by altering DNA-histone contacts within a nucleosome, leading eventually to a change in nucleosome position, thus facilitating or repressing binding of gene-specific transcription factors. Component of the histone acetyltransferase NuA3 complex, that acetylates Lys-14 of histone H3. Recruitment of NuA3 to nucleosomes requires methylated histone H3. In conjunction with the FACT complex, NuA3 may be involved in transcriptional regulation. Does not bind DNA. KEYWORDS: 3D-structure;Direct protein sequencing;Isopeptide bond;Nucleus;Reference proteome;Transcription;Transcription regulation;Ubl conjugation SUBCELLULAR LOCATION: Nucleus. MISCELLANEOUS: TAF14 is the only non-essential TAF.; MISCELLANEOUS: Present with 3100 molecules/cell in log phase SD medium.; MISCELLANEOUS: There is no homolog of TAF14 present in the TFIIF complexes of higher eukaryotes." P35197,"PROTEIN NAMES: ADP-ribosylation factor GTPase-activating protein GCS1 (ARF GAP GCS1) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: GTPase-activating protein (GAP) for ARF1 and ARF2. Involved in intracellular vesicular transport. Required for transport from the trans-Golgi network. Implicated in the regulation of retrograde transport from the Golgi to the ER and in actin cytoskeletal organization. May be involved in the maintenance of mitochondrial morphology, possibly through organizing the actin cytoskeleton in Saccharomyces. KEYWORDS: 3D-structure;Cytoplasm;Golgi apparatus;GTPase activation;Metal-binding;Mitochondrion;Phosphoprotein;Protein transport;Reference proteome;Transport;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cytoplasm Mitochondrion Cytoplasm, perinuclear region Golgi apparatus Note=Found also in the mitochondria and in the perinuclear region. MISCELLANEOUS: Present with 9560 molecules/cell in log phase SD medium." P35203,"PROTEIN NAMES: Centromere DNA-binding protein complex CBF3 subunit C (Chromosome transmission fidelity protein 13) (Kinetochore protein CTF13) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Acts as a central component of the centromere DNA-binding protein complex CBF3, which is essential for chromosome segregation and movement of centromeres along microtubules. CBF3 is required for the recruitment of other kinetochore complexes to CEN DNA. It plays a role in the attachment of chromosomes to the spindle and binds selectively to a highly conserved DNA sequence called CDEIII, found in centromers and in several promoters. The association of CBF3C with CBF3D and SGT1 is required for CBF3C activation and CBF3 assembly. KEYWORDS: 3D-structure;Centromere;Chromosome;Direct protein sequencing;DNA-binding;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus. Chromosome, centromere. " P35206,PROTEIN NAMES: Mannosyl phosphorylinositol ceramide synthase regulatory protein CSG2 ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Required for calcium regulation. May regulate calcium accumulation by a non-vacuole organelle. Also regulates the activity of CSH1 and SUR1 during mannosyl phosphorylinositol ceramide synthesis. KEYWORDS: Endoplasmic reticulum;Glycoprotein;Membrane;Reference proteome;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein P35329,"PROTEIN NAMES: B-cell receptor CD22 (B-lymphocyte cell adhesion molecule) (BL-CAM) (Sialic acid-binding Ig-like lectin 2) (Siglec-2) (T-cell surface antigen Leu-14) (CD antigen CD22) PROTEIN FAMILY: Immunoglobulin superfamily, SIGLEC (sialic acid binding Ig-like lectin) family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family. FUNCTION: Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules. KEYWORDS: Alternative splicing;Cell adhesion;Cell membrane;Disulfide bond;Glycoprotein;Immunoglobulin domain;Lectin;Membrane;Phosphoprotein;Reference proteome;Repeat;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. " P35352,PROTEIN NAMES: Cyclic AMP receptor 3 (cAMP receptor 3) PROTEIN FAMILY: G-protein coupled receptor 5 family ORGANISM: Dictyostelium discoideum (Social amoeba) SIMILARITY: Belongs to the G-protein coupled receptor 5 family. FUNCTION: Receptor for cAMP. Coordinates the aggregation of individual cells into a multicellular organism and regulates the expression of a large number of developmentally regulated genes. The activity of this receptor is mediated by G proteins. KEYWORDS: G-protein coupled receptor;Glycoprotein;Membrane;Phosphoprotein;Receptor;Reference proteome;Transducer;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. P35398,"PROTEIN NAMES: Nuclear receptor ROR-alpha (Nuclear receptor RZR-alpha) (Nuclear receptor subfamily 1 group F member 1) (RAR-related orphan receptor A) (Retinoid-related orphan receptor-alpha) PROTEIN FAMILY: Nuclear hormone receptor family, NR1 subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the nuclear hormone receptor family. NR1 subfamily. FUNCTION: Nuclear receptor that binds DNA as a monomer to ROR response elements (RORE) containing a single core motif half-site 5'-AGGTCA-3' preceded by a short A-T-rich sequence. Key regulator of embryonic development, cellular differentiation, immunity, circadian rhythm as well as lipid, steroid, xenobiotics and glucose metabolism. Considered to have intrinsic transcriptional activity, have some natural ligands like oxysterols that act as agonists (25-hydroxycholesterol) or inverse agonists (7-oxygenated sterols), enhancing or repressing the transcriptional activity, respectively. Recruits distinct combinations of cofactors to target genes regulatory regions to modulate their transcriptional expression, depending on the tissue, time and promoter contexts. Regulates genes involved in photoreceptor development including OPN1SW, OPN1SM and ARR3 and skeletal muscle development with MYOD1. Required for proper cerebellum development. Regulates SHH gene expression, among others, to induce granule cells proliferation as well as expression of genes involved in calcium-mediated signal transduction. Regulates the circadian expression of several clock genes, including CLOCK, BMAL1, NPAS2 and CRY1. Competes with NR1D1 for binding to their shared DNA response element on some clock genes such as BMAL1, CRY1 and NR1D1 itself, resulting in NR1D1-mediated repression or RORA-mediated activation of clock genes expression, leading to the circadian pattern of clock genes expression. Therefore influences the period length and stability of the clock. Regulates genes involved in lipid metabolism such as apolipoproteins APOA1, APOA5, APOC3 and PPARG. In liver, has specific and redundant functions with RORC as positive or negative modulator of expression of genes encoding phase I and phase II proteins involved in the metabolism of lipids, steroids and xenobiotics, such as CYP7B1 and SULT2A1. Induces a rhythmic expression of some of these genes. In addition, interplays functionally with NR1H2 and NR1H3 for the regulation of genes involved in cholesterol metabolism. Also involved in the regulation of hepatic glucose metabolism through the modulation of G6PC1 and PCK1. In adipose tissue, plays a role as negative regulator of adipocyte differentiation, probably acting through dual mechanisms. May suppress CEBPB-dependent adipogenesis through direct interaction and PPARG-dependent adipogenesis through competition for DNA-binding. Downstream of IL6 and TGFB and synergistically with RORC isoform 2, is implicated in the lineage specification of uncommitted CD4(+) T-helper (T(H)) cells into T(H)17 cells, antagonizing the T(H)1 program. Probably regulates IL17 and IL17F expression on T(H) by binding to the essential enhancer conserved non-coding sequence 2 (CNS2) in the IL17-IL17F locus. Involved in hypoxia signaling by interacting with and activating the transcriptional activity of HIF1A. May inhibit cell growth in response to cellular stress. May exert an anti-inflammatory role by inducing CHUK expression and inhibiting NF-kappa-B signaling. KEYWORDS: 3D-structure;Activator;Alternative promoter usage;Alternative splicing;Biological rhythms;Developmental protein;Disease variant;DNA-binding;Epilepsy;Intellectual disability;Isopeptide bond;Metal-binding;Methylation;Nucleus;Phosphoprotein;Receptor;Reference proteome;Transcription;Transcription regulation;Ubl conjugation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: [Isoform 2]: Produced by alternative promoter usage. Region from 23 to 71 inhibits DNA-binding and transactivation activity.; MISCELLANEOUS: [Isoform 3]: Produced by alternative splicing.; MISCELLANEOUS: [Isoform 4]: Produced by alternative promoter usage." P35401,"PROTEIN NAMES: Protein CRAC ORGANISM: Dictyostelium discoideum (Social amoeba) FUNCTION: Couples activated G protein to adenylyl cyclase signal transduction from surface cAMP receptor. Pianissimo a cytosolic regulator and CRAC, are both essential for activation of the enzyme adenylyl cyclase. Pianissimo and CRAC do not function redundantly. Both proteins are integral components of the adenylyl cyclase activation pathway. KEYWORDS: Cytoplasm;Developmental protein;Direct protein sequencing;Reference proteome SUBCELLULAR LOCATION: Cytoplasm. " P35524,"PROTEIN NAMES: Chloride channel protein 1 (ClC-1) (Chloride channel protein, skeletal muscle) PROTEIN FAMILY: Chloride channel family, ClC-1/CLCN1 subfamily ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the chloride channel (TC 2.A.49) family. ClC-1/CLCN1 subfamily. FUNCTION: Voltage-gated chloride channel. Plays an important role in membrane repolarization in skeletal muscle cells after muscle contraction (By similarity). KEYWORDS: CBS domain;Cell membrane;Chloride;Chloride channel;Ion channel;Ion transport;Membrane;Phosphoprotein;Reference proteome;Repeat;Transmembrane;Transmembrane helix;Transport;Voltage-gated channel SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein MISCELLANEOUS: The CLC channel family contains both chloride channels and proton-coupled anion transporters that exchange chloride or another anion for protons. The absence of conserved gating glutamate residues is typical for family members that function as channels (By similarity)." P35554,"PROTEIN NAMES: Flightin (Muscle protein 27) ORGANISM: Drosophila melanogaster (Fruit fly) FUNCTION: Possibly involved in the regulation of flight muscles contraction, possibly by modulating actin-myosin interaction. KEYWORDS: 3D-structure;Muscle protein;Phosphoprotein;Reference proteome " P35577,PROTEIN NAMES: Thyroxine-binding globulin (Serpin A7) (T4-binding globulin) PROTEIN FAMILY: Serpin family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the serpin family. FUNCTION: Major thyroid hormone transport protein in serum. KEYWORDS: Direct protein sequencing;Glycoprotein;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted. P35603,"PROTEIN NAMES: AP-2 complex subunit mu (Clathrin assembly protein complex 2 mu medium chain) (Clathrin coat assembly protein AP50) (Clathrin coat-associated protein AP50) (Mu2-adaptin) (Plasma membrane adaptor AP-2 50 kDa protein) (Protein dumpy-23) PROTEIN FAMILY: Adapter complexes medium subunit family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the adapter complexes medium subunit family. FUNCTION: Component of the adapter complexes which link clathrin to receptors in coated vesicles (By similarity). Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration (By similarity). AP50 is a subunit of the plasma membrane adapter (By similarity). Essential wnt/egl-20 signaling protein that functions in wnt/egl-20-producing cells. Required for the AP-2 complex-mediated endocytosis of membrane proteins including wntless homolog mig-14 in egl-20-producing cells. During development, regulates the migration of HSN neurons and the left and right Q neuroblasts (QL and QR, respectively) and their descendants, possibly through hox gene and wnt/egl-20 gene target mab-5, and plays a role in establishing ALM and PLM neuronal cell polarity. Regulates AWB sensory neuron cilia membrane expansion during development, potentially via localization of tub-1 and PtdIns(4,5)P2 to the ciliary base. Required for the asymmetric divisions of V5 cells. KEYWORDS: Alternative splicing;Cell membrane;Coated pit;Endocytosis;Membrane;Protein transport;Reference proteome;Transport SUBCELLULAR LOCATION: Cell membrane. Membrane, coated pit; Peripheral membrane protein; Cytoplasmic side. Note=Component of the coat surrounding the cytoplasmic face of coated vesicles in the plasma membrane. " P35621,PROTEIN NAMES: Protein DVR-1 PROTEIN FAMILY: TGF-beta family ORGANISM: Danio rerio (Zebrafish) (Brachydanio rerio) SIMILARITY: Belongs to the TGF-beta family. FUNCTION: Serves to facilitate the differentiation of either mesoderm or endoderm either as a cofactor in an instructive signal or by providing permissive environment. KEYWORDS: Cleavage on pair of basic residues;Disulfide bond;Glycoprotein;Growth factor;Mitogen;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted P35712,"PROTEIN NAMES: Transcription factor SOX-6 ORGANISM: Homo sapiens (Human) FUNCTION: Transcription factor that plays a key role in several developmental processes, including neurogenesis, chondrocytes differentiation and cartilage formation (Probable). Specifically binds the 5'-AACAAT-3' DNA motif present in enhancers and super-enhancers and promotes expression of genes important for chondrogenesis. Required for overt chondrogenesis when condensed prechondrocytes differentiate into early stage chondrocytes: SOX5 and SOX6 cooperatively bind with SOX9 on active enhancers and super-enhancers associated with cartilage-specific genes, and thereby potentiate SOX9's ability to transactivate. Not involved in precartilaginous condensation, the first step in chondrogenesis, during which skeletal progenitors differentiate into prechondrocytes. Together with SOX5, required to form and maintain a pool of highly proliferating chondroblasts between epiphyses and metaphyses, to form columnar chondroblasts, delay chondrocyte prehypertrophy but promote hypertrophy, and to delay terminal differentiation of chondrocytes on contact with ossification fronts. Binds to the proximal promoter region of the myelin protein MPZ gene, and is thereby involved in the differentiation of oligodendroglia in the developing spinal tube. Binds to the gene promoter of MBP and acts as a transcriptional repressor (By similarity). KEYWORDS: Activator;Alternative splicing;Autism spectrum disorder;Coiled coil;Cytoplasm;Developmental protein;Differentiation;Disease variant;DNA-binding;Intellectual disability;Isopeptide bond;Nucleus;Phosphoprotein;Reference proteome;Repressor;Transcription;Transcription regulation;Ubl conjugation SUBCELLULAR LOCATION: Nucleus Cytoplasm " P35713,"PROTEIN NAMES: Transcription factor SOX-18 ORGANISM: Homo sapiens (Human) FUNCTION: Transcriptional activator that binds to the consensus sequence 5'-AACAAAG-3' in the promoter of target genes and plays an essential role in embryonic cardiovascular development and lymphangiogenesis. Activates transcription of PROX1 and other genes coding for lymphatic endothelial markers. Plays an essential role in triggering the differentiation of lymph vessels, but is not required for the maintenance of differentiated lymphatic endothelial cells. Plays an important role in postnatal angiogenesis, where it is functionally redundant with SOX17. Interaction with MEF2C enhances transcriptional activation. Besides, required for normal hair development. KEYWORDS: Activator;Disease variant;DNA-binding;Hypotrichosis;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " P35728,"PROTEIN NAMES: Protein MPE1 ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Component of the cleavage and polyadenylation factor (CPF) complex, which plays a key role in polyadenylation-dependent pre-mRNA 3'-end formation and cooperates with cleavage factors including the CFIA complex and NAB4/CFIB. KEYWORDS: 3D-structure;Metal-binding;mRNA processing;Nucleus;Phosphoprotein;Reference proteome;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: Present with 1130 molecules/cell in log phase SD medium." P35790,"PROTEIN NAMES: Choline kinase alpha (CK) (CHETK-alpha) (Ethanolamine kinase) (EK) PROTEIN FAMILY: Choline/ethanolamine kinase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the choline/ethanolamine kinase family. FUNCTION: Plays a key role in phospholipid biosynthesis by catalyzing the phosphorylation of free choline to phosphocholine, the first step in phosphatidylcholine biosynthesis. Also phosphorylates ethanolamine, thereby contributing to phosphatidylethanolamine biosynthesis. Has higher activity with choline. May contribute to tumor cell growth.; FUNCTION: [Isoform 1]: This isoform plays a key role in lipolysis of lipid droplets following glucose deprivation. In response to glucose deprivation, phosphorylated by AMPK, promoting localization to lipid droplets. Phosphorylation is followed by acetylation by KAT5, leading to dissociation of the homodimer into a monomer. Monomeric CHKA isoform 1 is converted into a tyrosine-protein kinase, which phosphorylates lipid droplet structural proteins PLIN2 and PLIN3, leading to lipolysis of lipid droplets. PATHWAY: Phospholipid metabolism; phosphatidylcholine biosynthesis; phosphocholine from choline: step 1/1.; PATHWAY: Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from ethanolamine: step 1/3. KEYWORDS: 3D-structure;Acetylation;Alternative splicing;ATP-binding;Cytoplasm;Disease variant;Epilepsy;Intellectual disability;Kinase;Lipid biosynthesis;Lipid droplet;Lipid metabolism;Nucleotide-binding;Phospholipid biosynthesis;Phospholipid metabolism;Phosphoprotein;Reference proteome;Transferase;Tyrosine-protein kinase SUBCELLULAR LOCATION: Cytoplasm, cytosol.; SUBCELLULAR LOCATION: [Isoform 1]: Lipid droplet Note=Isoform 1 localizes to lipid droplets following phosphorylation by AMPK. " P35843,PROTEIN NAMES: Oxysterol-binding protein homolog 5 (Homologous to KES1 protein 1) (Protein HES1) (Oxysterol-binding protein-related protein 5) (ORP 5) (OSBP-related protein 5) PROTEIN FAMILY: OSBP family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the OSBP family. FUNCTION: Lipid transport protein (LTP) involved in non-vesicular transfer of lipids between membranes. Functions in phosphoinositide-coupled directional transport of various lipids by carrying the lipid molecule in a hydrophobic pocket and transferring it between membranes through the cytosol. Involved in maintenance of intracellular sterol distribution and homeostasis. Plays a role in ergosterol synthesis. Binds and transports sterol. May be involved in ergosterol transport from the plasma membrane (PM) to the ER. KEYWORDS: Lipid biosynthesis;Lipid metabolism;Membrane;Phosphoprotein;Reference proteome;Steroid biosynthesis;Steroid metabolism;Sterol biosynthesis;Sterol metabolism;Vacuole SUBCELLULAR LOCATION: Vacuole membrane Bud neck MISCELLANEOUS: Present with 1690 molecules/cell in log phase SD medium. P36000,"PROTEIN NAMES: AP-1 complex subunit beta-1 (Beta-1-adaptin) (Clathrin assembly protein complex 1 beta-1 large chain) (Clathrin assembly protein large beta-1 chain) PROTEIN FAMILY: Adaptor complexes large subunit family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the adaptor complexes large subunit family. FUNCTION: Adaptins are components of the adaptor complexes which link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration. The AP-1 complex interacts directly with clathrin. KEYWORDS: Cell membrane;Coated pit;Membrane;Protein transport;Reference proteome;Transport SUBCELLULAR LOCATION: Cell membrane. Membrane, coated pit; Peripheral membrane protein; Cytoplasmic side. Note=Component of the coat surrounding the cytoplasmic face of coated vesicles in the plasma membrane. MISCELLANEOUS: Present with 2690 molecules/cell in log phase SD medium." P36009,"PROTEIN NAMES: Probable ATP-dependent RNA helicase DHR2 (DEAH box RNA helicase DHR2) (Helicase JA2) PROTEIN FAMILY: DEAD box helicase family, DEAH subfamily ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the DEAD box helicase family. DEAH subfamily. FUNCTION: Probable ATP-binding RNA helicase. Required for 18S rRNA synthesis. KEYWORDS: ATP-binding;Helicase;Hydrolase;Nucleotide-binding;Nucleus;Reference proteome;RNA-binding;rRNA processing SUBCELLULAR LOCATION: Nucleus, nucleolus. MISCELLANEOUS: Present with 721 molecules/cell in log phase SD medium." P36013,"PROTEIN NAMES: NAD-dependent malic enzyme, mitochondrial (NAD-ME) PROTEIN FAMILY: Malic enzymes family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the malic enzymes family. KEYWORDS: Metal-binding;Mitochondrion;NAD;Oxidoreductase;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Mitochondrion matrix MISCELLANEOUS: Present with 10500 molecules/cell in log phase SD medium." P36081,"PROTEIN NAMES: PMA1 stabilization in the Golgi protein 1 [Cleaved into: PSG1-N'; PSG1-C'] ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: With EXP1, the specific cargo receptor protein for the plasma membrane ATPase PMA1, is involved in the transport and/or maturation of PMA1. EXP1 and PSG1 probably act sequentially to promote PMA1 sorting between the ER and the Golgi, with EXP1 promoting PMA1 export from the ER to the Golgi while PSG1 has a role in PMA1 maturation or quality control in the Golgi. PSG1 might also couple PMA1 sorting and maturation in the early secretory pathway with the glycosylation machinery (Probable).; FUNCTION: PSG1 is cleaved by KEX2 in two stable peptides, PSG1-N' and PSG1-C', the former supporting a role in maturation quality control, the latter having a role in modulating vesicular trafficking. KEYWORDS: Cytoplasmic vesicle;Glycoprotein;Golgi apparatus;Membrane;Reference proteome;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: [PSG1-N']: Golgi apparatus lumen.; SUBCELLULAR LOCATION: [PSG1-C']: Cytoplasmic vesicle, COPI-coated vesicle membrane ; Single-pass membrane protein MISCELLANEOUS: Present with 606 molecules/cell in log phase SD medium." P36084,PROTEIN NAMES: Splicing factor MUD2 ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Splicing factor that contacts pre-mRNA directly and is a component of the pre-mRNA-U1 snRNP complex (commitment complex 2) that forms during early spliceosome assembly in yeast extracts. KEYWORDS: mRNA processing;mRNA splicing;Phosphoprotein;Reference proteome;RNA-binding MISCELLANEOUS: Present with 4170 molecules/cell in log phase SD medium. P36096,"PROTEIN NAMES: Transmembrane E3 ubiquitin-protein ligase 1 (RING-type E3 ubiquitin transferase TUL1) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Catalytic component of the DSC E3 ubiquitin ligase complexes that tag proteins present in Golgi, endosome and vacuole membranes and function in protein homeostasis under non-stress conditions and support a role in protein quality control. Mediates ubiquitination of vacuolar proteins such as CPS1, PPN1, PEP12 and other proteins containing exposed hydrophilic residues within their transmembrane domains, leading to their sorting into internal vesicles in late endosomes. Targets also the unpalmitoylated endosomal SNARE TLG1 to the MVB pathway. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Golgi apparatus;Membrane;Metal-binding;Reference proteome;Signal;Transferase;Transmembrane;Transmembrane helix;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Golgi apparatus membrane ; Multi-pass membrane protein " P36100,PROTEIN NAMES: Transcription initiation factor IIE subunit alpha (TFIIE-alpha) (Factor A 66 kDa subunit) (Transcription factor A large subunit) PROTEIN FAMILY: TFIIE alpha subunit family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the TFIIE alpha subunit family. FUNCTION: Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase (By similarity). KEYWORDS: 3D-structure;Direct protein sequencing;Metal-binding;Nucleus;Reference proteome;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: Present with 35900 molecules/cell in log phase SD medium. P36113,PROTEIN NAMES: E3 ubiquitin-protein ligase HEL1 (Histone E3 ligase 1) (RING-type E3 ubiquitin transferase HEL1) PROTEIN FAMILY: RBR family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the RBR family. FUNCTION: Probable ubiquitin-protein ligase involved in the degradation-related ubiquitination of histones. Contributes to the post-translational regulation of histone protein levels by polyubiquitination of excess histones for subsequent degradation. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Metal-binding;Reference proteome;Repeat;Transferase;Ubl conjugation pathway;Zinc;Zinc-finger MISCELLANEOUS: Present with 2250 molecules/cell in log phase SD medium. P36122,"PROTEIN NAMES: Protein BCH2 (BUD7 and CHS6 homolog 2) PROTEIN FAMILY: CHAPS family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the CHAPS family. FUNCTION: Member of the CHS5-ARF1P-binding proteins (CHAPS) which mediates export of specific cargo proteins, including chitin synthase CHS3. KEYWORDS: Golgi apparatus;Membrane;Protein transport;Reference proteome;Transport SUBCELLULAR LOCATION: Golgi apparatus, trans-Golgi network membrane ; Peripheral membrane protein Note=Trans-Golgi network location requires interaction with CHS5 and with myristoylated GTP-bound ARF1 for the recruitment to the membranes. MISCELLANEOUS: Present with 2540 molecules/cell in log phase SD medium." P36124,"PROTEIN NAMES: SET domain-containing protein 3 PROTEIN FAMILY: SET3 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the SET3 family. FUNCTION: Transcriptional regulator that acts via the formation of large multiprotein complexes that modify and/or remodel the chromatin. Required for both gene activation and repression. Part of the Set3C complex, which is required to repress early/middle sporulation genes during meiosis. Required for the transcriptional activation of genes with high activity. KEYWORDS: 3D-structure;Activator;Chromatin regulator;Meiosis;Metal-binding;Phosphoprotein;Reference proteome;Repressor;Transcription;Transcription regulation;Zinc;Zinc-finger MISCELLANEOUS: Present with 623 molecules/cell in log phase SD medium." P36136,"PROTEIN NAMES: Sedoheptulose 1,7-bisphosphatase PROTEIN FAMILY: Phosphoglycerate mutase family, SHB17 subfamily ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the phosphoglycerate mutase family. SHB17 subfamily. FUNCTION: Sedoheptulose 1,7-bisphosphatase involved in riboneogenesis. Dephosphorylates sedoheptulose 1,7-bisphosphate (SBP), which is converted via the non-oxidative pentose phosphate pathway to ribose-5-phosphate. Has a fructose 1,6-bisphosphatase activity in vitro, but this is probably not biologically relevant, since deletion does not affect fructose 1,6-biphosphate (FBP) levels. KEYWORDS: 3D-structure;Carbohydrate metabolism;Cytoplasm;Hydrolase;Nucleus;Oxidoreductase;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Nucleus MISCELLANEOUS: Present with 11500 molecules/cell in log phase SD medium." P36166,PROTEIN NAMES: Paxillin-like protein 1 ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) KEYWORDS: LIM domain;Metal-binding;Phosphoprotein;Reference proteome;Repeat;Zinc MISCELLANEOUS: Present with 1310 molecules/cell in log phase SD medium. P36198,PROTEIN NAMES: LIM/homeobox protein Lhx2 (Homeobox protein LH-2) (LIM homeobox protein 2) ORGANISM: Rattus norvegicus (Rat) FUNCTION: Acts as a transcriptional activator. Stimulates the promoter of the alpha-glycoprotein gene. Transcriptional regulatory protein involved in the control of cell differentiation in developing lymphoid and neural cell types (By similarity). KEYWORDS: Activator;DNA-binding;Homeobox;LIM domain;Metal-binding;Nucleus;Reference proteome;Repeat;Transcription;Transcription regulation;Zinc SUBCELLULAR LOCATION: Nucleus P36234,PROTEIN NAMES: Glycerate dehydrogenase (GDH) (Glyoxylate reductase) (Hydroxypyruvate dehydrogenase) (NADH-dependent hydroxypyruvate reductase) (HPR) PROTEIN FAMILY: D-isomer specific 2-hydroxyacid dehydrogenase family ORGANISM: Hyphomicrobium methylovorum SIMILARITY: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. FUNCTION: Active on hydroxypyruvate and glyoxylate. PATHWAY: One-carbon metabolism; formaldehyde assimilation via serine pathway. KEYWORDS: 3D-structure;NAD;Oxidoreductase P36269,"PROTEIN NAMES: Glutathione hydrolase 5 proenzyme (Gamma-glutamyl transpeptidase-related enzyme) (GGT-rel) (Gamma-glutamyltransferase 5) (GGT 5) (Gamma-glutamyltransferase-like activity 1) (Gamma-glutamyltranspeptidase 5) (Leukotriene-C4 hydrolase) [Cleaved into: Glutathione hydrolase 5 heavy chain; Glutathione hydrolase 5 light chain] PROTEIN FAMILY: Gamma-glutamyltransferase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the gamma-glutamyltransferase family. FUNCTION: Cleaves the gamma-glutamyl peptide bond of glutathione and glutathione-S-conjugate such as leukotriene C4. Does not cleaves gamma-glutamyl compounds such as gamma-glutamyl leucine. May also catalyze a transpeptidation reaction in addition to the hydrolysis reaction, transferring the gamma-glutamyl moiety to an acceptor amino acid to form a new gamma-glutamyl compound. Acts as a negative regulator of geranylgeranyl glutathione bioactivity by cleaving off its gamma-glutamyl group, playing a role in adaptive immune responses. PATHWAY: Sulfur metabolism; glutathione metabolism.; PATHWAY: Lipid metabolism; leukotriene D4 biosynthesis. KEYWORDS: Acyltransferase;Alternative splicing;Glutathione biosynthesis;Glycoprotein;Hydrolase;Leukotriene biosynthesis;Membrane;Protease;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix;Zymogen SUBCELLULAR LOCATION: Membrane ; Single-pass type II membrane protein MISCELLANEOUS: A previous study reported that GSH and oxidized glutathione (GSSG) are not substrates for murine GGT5 (By similarity). However, this result contrasts with two studies reported that GSH is indeed a substrate for GGT5." P36406,"PROTEIN NAMES: E3 ubiquitin-protein ligase TRIM23 (ADP-ribosylation factor domain-containing protein 1) (GTP-binding protein ARD-1) (RING finger protein 46) (RING-type E3 ubiquitin transferase TRIM23) (Tripartite motif-containing protein 23) PROTEIN FAMILY: Small GTPase superfamily, Arf family ORGANISM: Homo sapiens (Human) SIMILARITY: In the C-terminal section; belongs to the small GTPase superfamily. Arf family. FUNCTION: Acts as an E3 ubiquitin-protein ligase. Plays an essential role in autophagy activation during viral infection. Mechanistically, activates TANK-binding kinase 1/TBK1 by facilitating its dimerization and ability to phosphorylate the selective autophagy receptor SQSTM1. In order to achieve this function, TRIM23 mediates 'Lys-27'-linked auto-ubiquitination of its ADP-ribosylation factor (ARF) domain to induce its GTPase activity and its recruitment to autophagosomes.; FUNCTION: (Microbial infection) Mediates TRAF6 auto-ubiquitination in the presence of human cytomegalovirus protein UL144, resulting in the virally controlled activation of NF-kappa-B stimulation at early times of HCMV infection. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: 3D-structure;Alternative splicing;Coiled coil;Cytoplasm;Golgi apparatus;GTP-binding;Host-virus interaction;Immunity;Innate immunity;Lysosome;Membrane;Metal-binding;Nucleotide-binding;Reference proteome;Transferase;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cytoplasm Endomembrane system Golgi apparatus membrane Lysosome membrane Note=Membrane-associated with the Golgi complex and lysosomal structures. " P36551,"PROTEIN NAMES: Oxygen-dependent coproporphyrinogen-III oxidase, mitochondrial (COX) (Coprogen oxidase) (Coproporphyrinogenase) PROTEIN FAMILY: Aerobic coproporphyrinogen-III oxidase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the aerobic coproporphyrinogen-III oxidase family. FUNCTION: Catalyzes the aerobic oxidative decarboxylation of propionate groups of rings A and B of coproporphyrinogen-III to yield the vinyl groups in protoporphyrinogen-IX and participates to the sixth step in the heme biosynthetic pathway. PATHWAY: Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; protoporphyrinogen-IX from coproporphyrinogen-III (O2 route): step 1/1. KEYWORDS: 3D-structure;Acetylation;Alternative splicing;Disease variant;Heme biosynthesis;Hereditary hemolytic anemia;Mitochondrion;Oxidoreductase;Phosphoprotein;Porphyrin biosynthesis;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Mitochondrion intermembrane space. " P36566,"PROTEIN NAMES: tRNA 5-carboxymethoxyuridine methyltransferase (cmo5U methyltransferase) PROTEIN FAMILY: Class I-like SAM-binding methyltransferase superfamily, CmoM family ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase superfamily. CmoM family. FUNCTION: Catalyzes the methylation of 5-carboxymethoxyuridine (cmo5U) to form 5-methoxycarbonylmethoxyuridine (mcmo5U) at position 34 in tRNAs. Four tRNAs (tRNA(Ala1), tRNA(Ser1), tRNA(Pro3) and tRNA(Thr4)) are fully modified with mcmo5U in stationary-phase E.coli. Also present at low frequency in tRNA(Leu3) and tRNA(Val1). KEYWORDS: 3D-structure;Methyltransferase;Reference proteome;S-adenosyl-L-methionine;Transferase;tRNA processing " P36600,PROTEIN NAMES: cAMP-dependent protein kinase regulatory subunit (PKA regulatory subunit) PROTEIN FAMILY: CAMP-dependent kinase regulatory chain family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the cAMP-dependent kinase regulatory chain family. KEYWORDS: cAMP;cAMP-binding;Nucleotide-binding;Phosphoprotein;Reference proteome;Repeat P36616,"PROTEIN NAMES: Protein kinase dsk1 (Dis1-suppressing protein kinase) PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. FUNCTION: May play an important role in mitotic control by altering cellular location, degree of phosphorylation and kinase activity. Abundant expression accelerates the exit when cells are in M-phase and also delays the entry into mitosis when cells are in G2. Phosphorylates prp2 in vitro and so may have a role in co-ordinating pre-mRNA splicing with the progression of the cell division cycle. KEYWORDS: ATP-binding;Cell cycle;Cell division;Cytoplasm;Kinase;Mitosis;Nucleotide-binding;Nucleus;Phosphoprotein;Reference proteome;Serine/threonine-protein kinase;Transferase SUBCELLULAR LOCATION: Cytoplasm Nucleus Note=Enriched in the nucleus in mitotically arrested mutant and wild-type mitotic cells but cytoplasmic enriched in cells at other cell-cycle stages. " P36617,"PROTEIN NAMES: DNA repair protein rad16 PROTEIN FAMILY: XPF family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the XPF family. FUNCTION: Endonuclease that specifically degrades single-stranded DNA and which is involved in nucleotide excision repair of DNA damaged with UV light, bulky adducts, or cross-linking agents. Required for double strand break-induced interchromosomal gene conversion. KEYWORDS: Cytoplasm;Cytoskeleton;DNA damage;DNA repair;DNA-binding;Endonuclease;Hydrolase;Nuclease;Nucleus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Nucleus Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body " P36621,"PROTEIN NAMES: Adenylyl cyclase-associated protein (CAP) PROTEIN FAMILY: CAP family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the CAP family. FUNCTION: The N-terminal domain binds to adenylyl cyclase, thereby enabling adenylyl cyclase to be activated by upstream regulatory signals, such as Ras. The C-terminal domain is required for normal cellular morphology and growth control. KEYWORDS: Phosphoprotein;Reference proteome " P36629,"PROTEIN NAMES: Splicing factor U2AF 59 kDa subunit (U2 auxiliary factor 59 kDa subunit) (U2AF59) (U2 snRNP auxiliary factor large subunit) PROTEIN FAMILY: Splicing factor SR family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the splicing factor SR family. FUNCTION: Necessary for the splicing of pre-mRNA. The SF1-U2AF59-U2AF23 complex has a role in the recognition of the branch site (5'-UACUAAC-3'), the pyrimidine tract and the 3'-splice site at the 3'-end of introns. KEYWORDS: 3D-structure;mRNA processing;mRNA splicing;Nucleus;Reference proteome;Repeat;RNA-binding SUBCELLULAR LOCATION: Nucleus " P36894,"PROTEIN NAMES: Bone morphogenetic protein receptor type-1A (BMP type-1A receptor) (BMPR-1A) (Activin receptor-like kinase 3) (ALK-3) (Serine/threonine-protein kinase receptor R5) (SKR5) (CD antigen CD292) PROTEIN FAMILY: Protein kinase superfamily, TKL Ser/Thr protein kinase family, TGFB receptor subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily. FUNCTION: On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for BMP2, BMP4, GDF5 and GDF6. Positively regulates chondrocyte differentiation through GDF5 interaction. Mediates induction of adipogenesis by GDF6. May promote the expression of HAMP, potentially via its interaction with BMP2 (By similarity). KEYWORDS: 3D-structure;ATP-binding;Cell membrane;Disease variant;Disulfide bond;Glycoprotein;Kinase;Magnesium;Manganese;Membrane;Metal-binding;Nucleotide-binding;Receptor;Reference proteome;Serine/threonine-protein kinase;Signal;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein Cell surface " P36923,"PROTEIN NAMES: 3-dehydroquinate dehydratase (3-dehydroquinase) (Type I DHQase) (Type I dehydroquinase) (DHQ1) PROTEIN FAMILY: Type-I 3-dehydroquinase family ORGANISM: Enterococcus faecalis (strain ATCC 700802 / V583) SIMILARITY: Belongs to the type-I 3-dehydroquinase family. FUNCTION: Involved in the third step of the chorismate pathway, which leads to the biosynthesis of aromatic amino acids (AroAA). Catalyzes the cis-dehydration of 3-dehydroquinate (DHQ) and introduces the first double bond of the aromatic ring to yield 3-dehydroshikimate. The reaction involves the formation of an imine intermediate between the keto group of 3-dehydroquinate and the epsilon-amino group of Lys-170 at the active site. PATHWAY: Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 3/7. KEYWORDS: 3D-structure;Amino-acid biosynthesis;Aromatic amino acid biosynthesis;Lyase;Reference proteome;Schiff base " P36924,"PROTEIN NAMES: Beta-amylase (1,4-alpha-D-glucan maltohydrolase) PROTEIN FAMILY: Glycosyl hydrolase 14 family ORGANISM: Bacillus cereus SIMILARITY: Belongs to the glycosyl hydrolase 14 family. KEYWORDS: 3D-structure;Calcium;Carbohydrate metabolism;Direct protein sequencing;Disulfide bond;Glycosidase;Hydrolase;Metal-binding;Polysaccharide degradation;Signal " P36941,"PROTEIN NAMES: Tumor necrosis factor receptor superfamily member 3 (Lymphotoxin-beta receptor) (Tumor necrosis factor C receptor) (Tumor necrosis factor receptor 2-related protein) (Tumor necrosis factor receptor type III) (TNF-RIII) (TNFR-III) ORGANISM: Homo sapiens (Human) FUNCTION: Receptor for the heterotrimeric lymphotoxin containing LTA and LTB, and for TNFS14/LIGHT. Promotes apoptosis via TRAF3 and TRAF5. May play a role in the development of lymphoid organs. KEYWORDS: 3D-structure;Alternative splicing;Apoptosis;Disulfide bond;Glycoprotein;Host-virus interaction;Membrane;Phosphoprotein;Receptor;Reference proteome;Repeat;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. " P36955,"PROTEIN NAMES: Pigment epithelium-derived factor (PEDF) (Cell proliferation-inducing gene 35 protein) (EPC-1) (Serpin F1) PROTEIN FAMILY: Serpin family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the serpin family. FUNCTION: Neurotrophic protein; induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity. KEYWORDS: 3D-structure;Direct protein sequencing;Dwarfism;Glycoprotein;Osteogenesis imperfecta;Phosphoprotein;Pyrrolidone carboxylic acid;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted Melanosome Note=Enriched in stage I melanosomes. " P37025,"PROTEIN NAMES: RNA 2',3'-cyclic phosphodiesterase (RNA 2',3'-CPDase) (Two-histidine 2',3'-cyclic phosphodiesterase acting on RNA) PROTEIN FAMILY: 2H phosphoesterase superfamily, ThpR family ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the 2H phosphoesterase superfamily. ThpR family. FUNCTION: Hydrolyzes RNA 2',3'-cyclic phosphodiester to an RNA 2'-phosphomonoester. In vitro, can also ligate 5' and 3' half-tRNA molecules with 2',3'-cyclic phosphate and 5'-hydroxyl termini, respectively, to the product containing the 2'-5' phosphodiester linkage. This reaction does not require ATP and is reversible. KEYWORDS: 3D-structure;Direct protein sequencing;Hydrolase;Reference proteome " P37058,"PROTEIN NAMES: 17-beta-hydroxysteroid dehydrogenase type 3 (17-beta-HSD 3) (Estradiol 17-beta-dehydrogenase 2) (Short chain dehydrogenase/reductase family 12C member 2) (Testicular 17-beta-hydroxysteroid dehydrogenase) (Testosterone 17-beta-dehydrogenase 3) PROTEIN FAMILY: Short-chain dehydrogenases/reductases (SDR) family, 17-beta-HSD 3 subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR) family. 17-beta-HSD 3 subfamily. FUNCTION: Catalyzes the conversion of 17-oxosteroids to 17beta-hydroxysteroids. Favors the reduction of androstenedione to testosterone. Testosterone is the key androgen driving male development and function. Uses NADPH while the two other EDH17B enzymes use NADH. Androgens such as epiandrosterone, dehydroepiandrosterone, androsterone and androstanedione are accepted as substrates and reduced at C-17. Can reduce 11-ketoandrostenedione as well as 11beta-hydroxyandrostenedione at C-17 to the respective testosterone forms. PATHWAY: Hormone biosynthesis; testosterone biosynthesis.; PATHWAY: Steroid metabolism. KEYWORDS: Alternative splicing;Disease variant;Endoplasmic reticulum;Lipid biosynthesis;Lipid metabolism;NADP;Oxidoreductase;Pseudohermaphroditism;Reference proteome;Steroid biosynthesis SUBCELLULAR LOCATION: Endoplasmic reticulum " P37217,"PROTEIN NAMES: Early activation antigen CD69 (CD antigen CD69) ORGANISM: Mus musculus (Mouse) FUNCTION: Involved in lymphocyte proliferation and functions as a signal transmitting receptor in lymphocytes, natural killer (NK) cells, and platelets. KEYWORDS: Disulfide bond;Glycoprotein;Lectin;Membrane;Phosphoprotein;Reference proteome;Signal-anchor;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane; Single-pass type II membrane protein. " P37366,"PROTEIN NAMES: Cyclin CCL1 PROTEIN FAMILY: Cyclin family, Cyclin C subfamily ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the cyclin family. Cyclin C subfamily. FUNCTION: Regulatory component of the TFIIK complex (KIN28-CCL1 dimer) which is the protein kinase component of transcription factor IIH (TFIIH) and phosphorylates the C-terminal domain of RNA polymerase II during transition from transcription to elongation after preinitiation complex (PIC) formation, thereby positively regulating transcription. TFIIH (or factor B) is essential for both basal and activated transcription, and is involved in nucleotide excision repair (NER) of damaged DNA. TFIIH has DNA-dependent ATPase activity and is essential for polymerase II transcription in vitro. KEYWORDS: 3D-structure;Cyclin;Reference proteome;Transcription;Transcription regulation MISCELLANEOUS: Present with 8700 molecules/cell in log phase SD medium." P37484,"PROTEIN NAMES: Cyclic-di-AMP phosphodiesterase GdpP (c-di-AMP phosphodiesterase YybT) (Cyclic-di-AMP hydrolase GdpP) (Cyclic-di-AMP phosphodiesterase YybT) PROTEIN FAMILY: GdpP/PdeA phosphodiesterase family ORGANISM: Bacillus subtilis (strain 168) SIMILARITY: Belongs to the GdpP/PdeA phosphodiesterase family. FUNCTION: Has phosphodiesterase (PDE) activity against cyclic-di-AMP (c-di-AMP) and to a much lesser extent against cyclic-di-GMP (c-di-GMP) in the DHH/DHHA1 domains. Also has ATPase activity, probably via the GGDEF domain. Overexpression leads to increased sensitivity to methyl methanesulfonate (MMS) and H(2)O(2). Overexpression leads to extreme sensitivity to the beta-lactam antibiotic cefuroxime (CEF), probably dependent on PDE activity. May monitor cellular heme or NO levels. In B.subtilis c-di-AMP is a second messenger that mediates growth, DNA repair and cell wall homeostasis; it is toxic when present in excess. KEYWORDS: Cell membrane;Heme;Hydrolase;Iron;Manganese;Membrane;Metal-binding;Reference proteome;Sporulation;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Note=After 3.5 hours of sporulation is present in a punctate pattern associated with the cell membrane. Does not co-localize with c-di-AMP synthase DisA. " P37529,"PROTEIN NAMES: Deoxyadenosine/deoxycytidine kinase (dAK/dCK) PROTEIN FAMILY: DCK/DGK family ORGANISM: Bacillus subtilis (strain 168) SIMILARITY: Belongs to the DCK/DGK family. FUNCTION: Plays an essential role in generating the deoxyribonucleotide precursors dATP and dCTP for DNA metabolism. The phosphate acceptor specificity is strict toward deoxyadenosine (dAdo) and deoxycytidine (dCyd). The specificity toward the sugar moiety of the nucleoside is less strict. Both 2-deoxyribose, ribose, and arabinose nucleosides are phosphorylated, although the 2-deoxyribonucleosides are preferred. The phosphate donor specificity is dependent on the deoxyribonucleoside substrate, but GTP is efficient with both deoxycytidine and deoxyadenosine. Only nucleoside triphosphates can act as phosphate donors. KEYWORDS: ATP-binding;Kinase;Nucleotide-binding;Reference proteome;Transferase " P37610,"PROTEIN NAMES: Alpha-ketoglutarate-dependent taurine dioxygenase (2-aminoethanesulfonate dioxygenase) (Sulfate starvation-induced protein 3) (SSI3) PROTEIN FAMILY: TfdA dioxygenase family ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the TfdA dioxygenase family. FUNCTION: Catalyzes the alpha-ketoglutarate-dependent hydroxylation of taurine yielding sulfite and aminoacetaldehyde after decomposition of an unstable intermediate. Is required for the utilization of taurine (2-aminoethanesulfonate) as an alternative sulfur source for growth in the absence of sulfate. To a lesser extent, pentanesulfonate, 3-(N-morpholino)propanesulfonate and 1,3-dioxo-2-isoindolineethanesulfonate are also desulfonated by this enzyme in vitro; however, desulfonation by TauD of organosulfonates other than taurine seem to be of little or no importance for sulfur metabolism in vivo. PATHWAY: Organosulfur degradation; taurine degradation via aerobic pathway; aminoacetaldehyde and sulfite from taurine: step 1/1. KEYWORDS: 3D-structure;Dioxygenase;Direct protein sequencing;Hydroxylation;Iron;Metal-binding;Oxidoreductase;Reference proteome;Vitamin C " P37613,"PROTEIN NAMES: PanD regulatory factor PROTEIN FAMILY: PanZ/PanM family ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the PanZ/PanM family. FUNCTION: Controls both the activation and catalytic activity of PanD in a coenzyme A (CoA)-dependent fashion. Binding of CoA or a derivative to PanZ leads to interaction with PanD, which promotes the processing and activation of pro-PanD, and subsequent substrate-mediated inhibition of the active form of PanD. Inhibition of PanD activity is probably the primary metabolic role of PanZ, allowing negative feedback regulation of pantothenate biosynthesis by CoA. KEYWORDS: 3D-structure;Pantothenate biosynthesis;Reference proteome " P37617,"PROTEIN NAMES: Zinc/cadmium/lead-transporting P-type ATPase (Pb(II)/Cd(II)/Zn(II)-translocating ATPase) (Zn(2+)/Cd(2+)/Pb(2+) export ATPase) PROTEIN FAMILY: Cation transport ATPase (P-type) family, Type IB subfamily ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily. FUNCTION: Confers resistance to zinc, cadmium and lead. Couples the hydrolysis of ATP with the export of zinc, cadmium or lead, with highest activity when the metals are present as metal-thiolate complexes. Can also bind nickel, copper, cobalt and mercury. KEYWORDS: 3D-structure;ATP-binding;Cadmium;Cell inner membrane;Cell membrane;Ion transport;Lead;Magnesium;Membrane;Metal-binding;Nucleotide-binding;Phosphoprotein;Reference proteome;Translocase;Transmembrane;Transmembrane helix;Transport;Zinc;Zinc transport SUBCELLULAR LOCATION: Cell inner membrane ; Multi-pass membrane protein " P37773,"PROTEIN NAMES: UDP-N-acetylmuramate--L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptandioate ligase (Murein peptide ligase) (UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-meso-diaminopimelate ligase) PROTEIN FAMILY: MurCDEF family, Mpl subfamily ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the MurCDEF family. Mpl subfamily. FUNCTION: Reutilizes the intact tripeptide L-alanyl-gamma-D-glutamyl-meso-diaminopimelate by linking it to UDP-N-acetylmuramate. The enzyme can also use the tetrapeptide L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptanedioyl-D-alanine or the pentapeptide L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptandioyl-D-alanyl-D-alanine in vivo and in vitro. PATHWAY: Cell wall biogenesis; peptidoglycan recycling. KEYWORDS: ATP-binding;Cell cycle;Cell division;Cell shape;Cell wall biogenesis/degradation;Ligase;Magnesium;Nucleotide-binding;Peptidoglycan synthesis;Reference proteome;Secreted SUBCELLULAR LOCATION: Secreted. " P37965,"PROTEIN NAMES: Glycerophosphodiester phosphodiesterase (Glycerophosphoryl diester phosphodiesterase) (Teichoicase) PROTEIN FAMILY: Glycerophosphoryl diester phosphodiesterase family ORGANISM: Bacillus subtilis (strain 168) SIMILARITY: Belongs to the glycerophosphoryl diester phosphodiesterase family. FUNCTION: Glycerophosphodiester phosphodiesterase hydrolyzes glycerophosphodiesters into glycerol-3-phosphate (G3P) and the corresponding alcohol. Involved in wall teichoic acid (WTA) metabolism during phosphate starvation. Catalyzes the degradation of WTA, enabling the utilization of WTA as a phosphate reserve under limiting conditions. Is highly selective for the poly(gylcerol phosphate) WTA backbone and catalyzes exolytic cleavage of individual monomer units. In vitro is active toward the WTA oligomer mimics glycerophosphoglycerol (GPG) and bis-glycerophosphoglycerol (bGPG). KEYWORDS: 3D-structure;Calcium;Cell wall biogenesis/degradation;Glycerol metabolism;Hydrolase;Metal-binding;Reference proteome;Secreted;Signal;Stress response SUBCELLULAR LOCATION: Secreted " P38032,"PROTEIN NAMES: L-aspartate oxidase (LASPO) (L-aspartate:fumarate oxidoreductase) (Quinolinate synthase B) PROTEIN FAMILY: FAD-dependent oxidoreductase 2 family, NadB subfamily ORGANISM: Bacillus subtilis (strain 168) SIMILARITY: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily. FUNCTION: Catalyzes the oxidation of L-aspartate to iminoaspartate, the first step in the de novo biosynthesis of NAD(+). Can use either oxygen or fumarate as electron acceptors, which allows the enzyme to be functional under aerobic and anaerobic conditions. PATHWAY: Cofactor biosynthesis; NAD(+) biosynthesis; iminoaspartate from L-aspartate (oxidase route): step 1/1. KEYWORDS: Cytoplasm;FAD;Flavoprotein;Nucleotide-binding;Oxidoreductase;Pyridine nucleotide biosynthesis;Reference proteome SUBCELLULAR LOCATION: Cytoplasm " P38041,PROTEIN NAMES: Protein BOB1 (BEM1-binding protein) (Growth inhibitory protein 7) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Binds to the BEM1 protein. KEYWORDS: Phosphoprotein;Reference proteome;SH3 domain MISCELLANEOUS: Present with 1040 molecules/cell in log phase SD medium. P38051,"PROTEIN NAMES: Isochorismate synthase MenF (Isochorismate hydroxymutase) (Isochorismate mutase) PROTEIN FAMILY: Isochorismate synthase family ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the isochorismate synthase family. FUNCTION: Catalyzes the conversion of chorismate to isochorismate. Can also catalyze the reverse reaction, but with a lower efficiency. PATHWAY: Quinol/quinone metabolism; 1,4-dihydroxy-2-naphthoate biosynthesis; 1,4-dihydroxy-2-naphthoate from chorismate: step 1/7.; PATHWAY: Quinol/quinone metabolism; menaquinone biosynthesis. KEYWORDS: 3D-structure;Isomerase;Magnesium;Menaquinone biosynthesis;Metal-binding;Reference proteome " P38069,"PROTEIN NAMES: Alpha-1,2-mannosyltransferase MNN2 (Calcium resistance and vanadate sensitivity protein 4) (Mannan synthesis protein MNN2) PROTEIN FAMILY: MNN1/MNT family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the MNN1/MNT family. FUNCTION: Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides. PATHWAY: Protein modification; protein glycosylation. KEYWORDS: Glycoprotein;Golgi apparatus;Membrane;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus membrane ; Single-pass type II membrane protein Note=Golgi localization depends on SVP26. MISCELLANEOUS: Present with 6730 molecules/cell in log phase SD medium." P38070,"PROTEIN NAMES: Serine/threonine-protein kinase YPK3 (Ribosomal S6 kinase homolog YPK3) (S6K homolog YPK3) PROTEIN FAMILY: Protein kinase superfamily, AGC Ser/Thr protein kinase family, S6 kinase subfamily ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily. FUNCTION: AGC kinase which plays a role in TOR complex 1 (TORC1) signaling pathway which mediates temporal control of cell growth in response to nutrients. Required for phosphorylation of ribosomal protein S6 (RPS6A/RPS6B) at 'Ser-232' and 'Ser-233'. KEYWORDS: ATP-binding;Cytoplasm;Kinase;Nucleotide-binding;Phosphoprotein;Reference proteome;Serine/threonine-protein kinase;Transferase SUBCELLULAR LOCATION: Cytoplasm MISCELLANEOUS: Present with 1470 molecules/cell in log phase SD medium." P38086,"PROTEIN NAMES: DNA repair and recombination protein RDH54 (RAD homolog 54) (Recombination factor TID1) (Two hybrid interaction with DMC1 protein 1) PROTEIN FAMILY: SNF2/RAD54 helicase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the SNF2/RAD54 helicase family. FUNCTION: Involved in the recombinational repair of double-strand breaks (DSB) in DNA during mitosis and meiosis. Has DNA dependent ATPase activity. Promotes D-loop (displacement loop) formation with RAD51 recombinase. Modifies the topology of double-stranded DNA during the D-loop reaction to facilitate the invasion of the homologous duplex molecule by the initiating single-stranded DNA substrate. Required for adaptation from G2/M checkpoint arrest induced by a double strand break, by participating in monitoring the extent of single-stranded DNA produced by resection of DNA ends. This role is distinct from its roles in recombination. Promotes colocalization of RAD51 and DMC1 during meiotic recombination. Involved in crossover interference. KEYWORDS: ATP-binding;DNA damage;DNA recombination;DNA repair;DNA-binding;Helicase;Hydrolase;Isopeptide bond;Meiosis;Nucleotide-binding;Nucleus;Reference proteome;Ubl conjugation SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: Present with 1270 molecules/cell in log phase SD medium." P38135,"PROTEIN NAMES: Medium-chain fatty-acid--CoA ligase (Acyl-CoA synthetase) (ACS) (Fatty acyl-CoA synthetase FadK) PROTEIN FAMILY: ATP-dependent AMP-binding enzyme family ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the ATP-dependent AMP-binding enzyme family. FUNCTION: Catalyzes the esterification, concomitant with transport, of exogenous fatty acids into metabolically active CoA thioesters for subsequent degradation or incorporation into phospholipids. Is maximally active on C6:0, C8:0 and C12:0 fatty acids, while has a low activity on C14-C18 chain length fatty acids. Is involved in the anaerobic beta-oxidative degradation of fatty acids, which allows anaerobic growth of E.coli on fatty acids as a sole carbon and energy source in the presence of nitrate or fumarate as a terminal electron acceptor. Can functionally replace FadD under anaerobic conditions. PATHWAY: Lipid metabolism; fatty acid beta-oxidation. KEYWORDS: ATP-binding;Cell membrane;Fatty acid metabolism;Ligase;Lipid metabolism;Magnesium;Membrane;Nucleotide-binding;Reference proteome SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein. Note=Partially membrane-associated. MISCELLANEOUS: Probably starts on Met-1; overexpressed protein starting at Met-19 has lower activity, forms aggregates during reaction and is unstable in storage at -80 degrees Celsius.; MISCELLANEOUS: The enzymatic mechanism is a two-step reaction that proceeds via the intermediate formation of an acyl-adenylate (acyl-AMP) intermediate." P38137,PROTEIN NAMES: Oxalate--CoA ligase (Acyl-activating enzyme 3) (Oxalyl-CoA synthetase) (Peroxisomal-coenzyme A synthetase) PROTEIN FAMILY: ATP-dependent AMP-binding enzyme family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the ATP-dependent AMP-binding enzyme family. FUNCTION: Catalyzes the first step in a degradation pathway of oxalate to CO(2) to protect the cell against the harmful effects of oxalate derived from endogenous processes or an environmental sources. KEYWORDS: 3D-structure;ATP-binding;Direct protein sequencing;Ligase;Membrane;Nucleotide-binding;Peroxisome;Reference proteome SUBCELLULAR LOCATION: Peroxisome matrix Peroxisome membrane ; Peripheral membrane protein Note=Imported in peroxisome via recognition by the peroxisomal targeting signal receptor PEX5. MISCELLANEOUS: Present with 8770 molecules/cell in log phase SD medium. P38139,"PROTEIN NAMES: Lipid droplet hydrolase 1 (Lipid esterase) (Triacylglycerol lipase) PROTEIN FAMILY: AB hydrolase superfamily, Lipase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the AB hydrolase superfamily. Lipase family. FUNCTION: Serine hydrolase required for the maintenance of steady state level of non-polar and polar lipids of lipid droplets and thus plays a role in maintaining the lipids homeostasis. Exhibits both esterase and triacylglycerol lipase activity. KEYWORDS: Hydrolase;Lipid degradation;Lipid droplet;Lipid metabolism;Reference proteome SUBCELLULAR LOCATION: Lipid droplet " P38207,PROTEIN NAMES: DNA-(apurinic or apyrimidinic site) endonuclease 2 (AP endonuclease 2) (Apurinic-apyrimidinic endonuclease 2) PROTEIN FAMILY: DNA repair enzymes AP/ExoA family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the DNA repair enzymes AP/ExoA family. FUNCTION: DNA repair enzyme that cleaves apurinic/apyrimidinic (AP) sites and removes 3'-blocking groups present at single strand breaks of damaged DNA. KEYWORDS: 3D-structure;DNA damage;DNA repair;Hydrolase;Magnesium;Metal-binding;Nucleus;Reference proteome;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus. MISCELLANEOUS: Present with 414 molecules/cell in log phase SD medium. P38211,PROTEIN NAMES: GPI mannosyltransferase 2 (GPI mannosyltransferase II) (GPI-MT-II) (Glycosylphosphatidylinositol-anchor biosynthesis protein 18) PROTEIN FAMILY: PIGV family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the PIGV family. FUNCTION: Mannosyltransferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Responsible for the transfer of the second mannose to the glycosylphosphatidylinositol during GPI precursor assembly. PATHWAY: Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis. KEYWORDS: Endoplasmic reticulum;Glycoprotein;Glycosyltransferase;GPI-anchor biosynthesis;Membrane;Reference proteome;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein P38221,"PROTEIN NAMES: Phosphatidate cytidylyltransferase (CDP-diacylglycerol synthase) (CDP-DAG synthase) (CDS) (CDP-diglyceride pyrophosphorylase) (CDP-diglyceride synthase) (CDP-DG synthase) (CTP:phosphatidate cytidylyltransferase) PROTEIN FAMILY: CDS family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the CDS family. FUNCTION: Supplies CDP-diacylglycerol, which may play an important role as both a precursor to phosphoinositide biosynthesis in the plasma membrane and as a negative effector of phosphatidylinositol 4-kinase activity, thereby exerting an effect on cell proliferation via a lipid-dependent signal transduction cascade. PATHWAY: Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 3/3. KEYWORDS: Cytoplasmic vesicle;Endoplasmic reticulum;Lipid biosynthesis;Lipid metabolism;Magnesium;Membrane;Nucleotidyltransferase;Phospholipid biosynthesis;Phospholipid metabolism;Reference proteome;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass membrane protein. Cytoplasmic vesicle, secretory vesicle. Note=Exclusively present in the ER, and not in mitochondria. Also associated with post-Golgi apparatus secretory vesicles destined for the plasma membrane. " P38238,"PROTEIN NAMES: tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase (2'-O-ribose RNA methyltransferase TRM7) (tRNA methylase 7) PROTEIN FAMILY: Class I-like SAM-binding methyltransferase superfamily, RNA methyltransferase RlmE family, TRM7 subfamily ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA methyltransferase RlmE family. TRM7 subfamily. FUNCTION: Methylates the 2'-O-ribose of nucleotides at positions 32 and 34 of the tRNA anticodon loop of substrate tRNAs. Requisite for faithful cytoplasmic translation (By similarity). Requires TRM732 for methylation of the cytidine at position 32 of the anticodon loop of substrate tRNAs. Requires RTT10/TRM734 for methylation of the nucleotide at position 34 of the anticodon loop of substrate tRNAs. May modify position 32 in tRNA(Leu(UAA)), tRNA(Phe(GAA)), and tRNA(Trp(CCA)) and position 34 in tRNA(Leu(UAA)), tRNA(Phe(GAA)) and tRNA(Trp(CCA)). Methylation of tRNA(Phe) at position 34 plays a role in the oxidative stress-response as it may promote translation of UUC over UUU, and UUC-containing genes are enriched for oxidative stress-responsive processes. KEYWORDS: 3D-structure;Cytoplasm;Methyltransferase;Reference proteome;S-adenosyl-L-methionine;Transferase;tRNA processing SUBCELLULAR LOCATION: Cytoplasm MISCELLANEOUS: Present with 4110 molecules/cell in log phase SD medium." P38242,PROTEIN NAMES: UDP-N-acetylglucosamine transferase subunit ALG14 (Asparagine-linked glycosylation protein 14) PROTEIN FAMILY: ALG14 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the ALG14 family. FUNCTION: Involved in protein N-glycosylation. Essential for the second step of the dolichol-linked oligosaccharide pathway. Anchors the catalytic subunit ALG13 to the ER. KEYWORDS: Endoplasmic reticulum;Membrane;Nucleus;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Single-pass membrane protein Nucleus membrane ; Single-pass membrane protein MISCELLANEOUS: Present with 339 molecules/cell in log phase SD medium. P38250,PROTEIN NAMES: Increased sodium tolerance protein 2 ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: May be involved in ion homeostasis together with BTN1 or BTN2. KEYWORDS: Cell membrane;Membrane;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Note=Correct localization requires BTN2. Localizes to the mother cell in small budded cells and to the bud in medium and large budded cells. Transported to the bud tip by an actomyosin based process. Compartmentalization maintained by a septin mediated membrane diffusion barrier at the mother-bud neck. P38262,PROTEIN NAMES: SIR4-interacting protein SIF2 ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Antagonizes telomeric silencing in yeast. May recruit SIR4 to non-telomeric sites or repression. KEYWORDS: 3D-structure;Chromatin regulator;Nucleus;Phosphoprotein;Reference proteome;Repeat;WD repeat SUBCELLULAR LOCATION: Nucleus. MISCELLANEOUS: Present with 1620 molecules/cell in log phase SD medium. P38263,"PROTEIN NAMES: Vacuolar import and degradation protein 24 (Glucose-induced degradation protein 4) PROTEIN FAMILY: GID4/VID24 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the GID4/VID24 family.; SIMILARITY: To yeast YGR066c and S.pombe SpAC3H1.14. FUNCTION: Substrate-recognition component of the GID complex, a multisubunit ubiquitin ligase that targets enzymes involved in gluconeogenesis for proteasomal degradation when cells are shifted to glucose-containing medium. Specific for substrates with an N-terminal Pro (Pro/N-degron), including FBP1, ICL1 and MDH2. Has high affinity for the N-terminal sequence Pro-Thr-Leu-Val, and can bind peptides with an N-terminal sequence of the type Pro-[Gly,Ala,Ser,Thr,Asp,Asn,Tyr,His]-[Ala,Val,Leu,Ile,Lys,Arg]-[Val,Cys,Pro,Leu,Ile,Trp]. Required for vacuolar degradation of FBP1 when cells are shifted to glucose-containing medium, probably by targeting FBP1-containing vesicles to the vacuole, but is not required for FBP1 sequestration in cytoplasmic vesicles. KEYWORDS: 3D-structure;Cytoplasmic vesicle;Isopeptide bond;Membrane;Phosphoprotein;Protein transport;Reference proteome;Transport;Ubl conjugation;Ubl conjugation pathway SUBCELLULAR LOCATION: Cytoplasmic vesicle membrane ; Peripheral membrane protein Note=Colocalizes with FBPase-containing vesicles. MISCELLANEOUS: Present with 6650 molecules/cell in log phase SD medium." P38273,"PROTEIN NAMES: Vacuolar fusion protein CCZ1 (Ccz1p) (Autophagy-related protein 11) (Calcium-caffeine-zinc sensitivity protein 1) (Cytoplasm to vacuole targeting protein 16) PROTEIN FAMILY: CCZ1 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the CCZ1 family. FUNCTION: In complex with MON1, is required for multiple vacuole delivery pathways including the cytoplasm to vacuole transport (Cvt), autophagy, pexophagy and endocytosis. The MON1-CCZ1 complex acts at the fusion of vesicles with the vacuole, through its regulation of the SNARE complex during the coordinated priming and docking stages of fusion, and particularly at the stage of tethering/docking. The MON1-CCZ1 complex is recruited to membranes enriched in charged lipids, particularly phosphatidylinositol 3-phosphate (PtdIns3P), by GTP-associated small GTPase RAB5 homologs (YPT10, YPT52, YPT53 and VPS21). The MON1-CCZ1 complex recruits GDP-associated small GTPase YPT7 to membranes and acts as a guanine nucleotide-exchange factor (GEF), promoting nucleotide exchange on YPT7 and triggering endosomal maturation by recruiting downstream effectors such as components of the HOPS tethering complex. KEYWORDS: Autophagy;Endosome;Membrane;Protein transport;Reference proteome;Transport;Vacuole SUBCELLULAR LOCATION: Endosome, multivesicular body membrane; Peripheral membrane protein. Prevacuolar compartment membrane; Peripheral membrane protein. Vacuole membrane; Peripheral membrane protein. Vesicle Note=The association of the MON1-CCZ1 complex with the vacuole is regulated by the C-Vps/HOPS complex. Colocalizes with the small GTPase RAB5 homolog YPT10 to vesicular structures near the vacuole. MISCELLANEOUS: Present with 2870 molecules/cell in log phase SD medium." P38291,"PROTEIN NAMES: Ribonucleases P/MRP protein subunit POP7 (RNA-processing protein POP7) (RNases P/MRP 15.8 kDa subunit) PROTEIN FAMILY: Histone-like Alba family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the histone-like Alba family. FUNCTION: Component of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends. Also a component of RNase MRP, which cleaves pre-rRNA sequences. KEYWORDS: 3D-structure;Hydrolase;Nucleus;Phosphoprotein;Reference proteome;rRNA processing;tRNA processing SUBCELLULAR LOCATION: Nucleus " P38297,"PROTEIN NAMES: Mitofusin FZO1 (Transmembrane GTPase FZO1) PROTEIN FAMILY: TRAFAC class dynamin-like GTPase superfamily, Dynamin/Fzo/YdjA family, Mitofusin subfamily ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family. Mitofusin subfamily. FUNCTION: Essential transmembrane GTPase, which mediates mitochondrial fusion. Fusion proceeds through several steps; first mitochondria are tethered together, then brought into close contact, followed by the formation of a docking ring around contact areas, and finally membrane fusion. Fusion of mitochondria occurs in many cell types and constitutes an important step in mitochondrial morphology, which is balanced between fusion and fission, mediated by FZO1 and DNM1, respectively. Functions antagonistically with DNM1. Probably acts by forming membrane contact sites that mediate mitochondrial membrane fusion. Mitochondrial docking and fusion requires GTP hydrolysis. Mitochondrial fusion promotes also increased lifespan. KEYWORDS: Coiled coil;GTP-binding;Hydrolase;Isopeptide bond;Membrane;Mitochondrion;Mitochondrion outer membrane;Nucleotide-binding;Reference proteome;Transmembrane;Transmembrane helix;Ubl conjugation SUBCELLULAR LOCATION: Mitochondrion outer membrane ; Multi-pass membrane protein Note=Detected at the periphery of mitochondrial contact sites that form before mitochondrial fusion. MISCELLANEOUS: Present with 1000 molecules/cell in log phase SD medium." P38310,PROTEIN NAMES: Iron transporter FTH1 PROTEIN FAMILY: Oxidase-dependent Fe transporter (OFeT) family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the oxidase-dependent Fe transporter (OFeT) (TC 9.A.10.1) family. FUNCTION: High affinity iron transporter probably involved in transport of intravacuolar stores of iron. KEYWORDS: Ion transport;Iron;Iron transport;Membrane;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix;Transport;Vacuole SUBCELLULAR LOCATION: Vacuole membrane ; Multi-pass membrane protein MISCELLANEOUS: Present with 486 molecules/cell in log phase SD medium. P38332,"PROTEIN NAMES: Diphthine methyltransferase (Diphthamide biosynthesis protein 7) (Endosomal recycling protein 1) (Regulator of rDNA transcription protein 2) PROTEIN FAMILY: DPH7 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the DPH7 family. FUNCTION: Catalyzes the demethylation of diphthine methyl ester to form diphthine, an intermediate in diphthamide biosynthesis, a post-translational modification of histidine which occurs in translation elongation factor 2 (EFT1 and EFT2). Also plays a role in the regulation of the retromer complex and is required for the recycling from endosomes of plasma membrane proteins like CAN1 and MUP1. Identified in a screen for mutants with decreased levels of rDNA transcription. PATHWAY: Protein modification; peptidyl-diphthamide biosynthesis. KEYWORDS: Cytoplasm;Endosome;Hydrolase;Reference proteome;Repeat;WD repeat SUBCELLULAR LOCATION: Cytoplasm Endosome Note=Recruited to endosomes in cells in which increased recycling of internalized plasma membrane proteins occurs. MISCELLANEOUS: Present with 2640 molecules/cell in log phase SD medium." P38334,"PROTEIN NAMES: Trafficking protein particle complex subunit 20 (TRAPP subunit 20) (Transport protein particle 20 kDa subunit) PROTEIN FAMILY: TRAPP small subunits family, Sedlin subfamily ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the TRAPP small subunits family. Sedlin subfamily. FUNCTION: Component of the TRAPP I, TRAPP II and TRAPP III complexes which act as guanine nucleotide exchange factors (GEF) for YPT1. TRAPP I plays a key role in the late stages of endoplasmic reticulum to Golgi traffic. TRAPP II plays a role in intra-Golgi transport. TRAPP III plays a role in autophagosome formation. KEYWORDS: 3D-structure;Autophagy;Endoplasmic reticulum;ER-Golgi transport;Golgi apparatus;Reference proteome;Transport SUBCELLULAR LOCATION: Golgi apparatus, cis-Golgi network. Endoplasmic reticulum. Preautophagosomal structure. MISCELLANEOUS: Present with 1200 molecules/cell in log phase SD medium." P38336,PROTEIN NAMES: RNases MRP/P 32.9 kDa subunit (RNA-processing protein POP4) PROTEIN FAMILY: Eukaryotic/archaeal RNase P protein component 1 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the eukaryotic/archaeal RNase P protein component 1 family. FUNCTION: Required for 5.8S rRNA and tRNA processing; associated with RNase MRP and RNase P. KEYWORDS: 3D-structure;Nucleus;Phosphoprotein;Reference proteome;rRNA processing;tRNA processing SUBCELLULAR LOCATION: Nucleus P38339,"PROTEIN NAMES: RHO GTPase-activating protein RGD1 (RhoGAP) (Related GAP domain protein 1) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: GTPase activating protein of RHO3 and RHO4. Acts in concert with MID2 in cell integrity, which is functionally linked to the PKC pathway. Involved in various stress responses. Required at low pH for activation of the PKC pathway. Important during mating response. KEYWORDS: 3D-structure;Coiled coil;Cytoplasm;GTPase activation;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Note=Bud and bud neck. During isotropic bud growth in the G1 and S phases, detected at the bud tip. During cytokinesis, undetectable at the bud tip and localized at the bud neck only. MISCELLANEOUS: Present with 1500 molecules/cell in log phase SD medium.; MISCELLANEOUS: Lack of RGD1 diminishes the PKC pathway activity." P38348,"PROTEIN NAMES: DNA mismatch repair protein HSM3 (Enhanced spontaneous mutability protein 3) PROTEIN FAMILY: Proteasome subunit S5B/HSM3 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the proteasome subunit S5B/HSM3 family. FUNCTION: Involved in DNA mismatch repair in slow-growing cells. Acts as a chaperone during the assembly of the 26S proteasome, specifically of the base subcomplex of the 19S regulatory complex (RC). KEYWORDS: 3D-structure;Chaperone;Cytoplasm;DNA damage;DNA repair;Reference proteome SUBCELLULAR LOCATION: Cytoplasm MISCELLANEOUS: Present with 468 molecules/cell in log phase SD medium." P38352,PROTEIN NAMES: SCF-associated factor 1 ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Targets AAH1 adenine deaminase for proteasome-dependent degradation upon entry into quiescence. Targets also URA7. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Phosphoprotein;Reference proteome;Repeat;Ubl conjugation pathway P38353,"PROTEIN NAMES: Sec sixty-one protein homolog (Ssh1 complex subunit SSH1) (Ssh1 complex subunit alpha) PROTEIN FAMILY: SecY/SEC61-alpha family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the SecY/SEC61-alpha family. FUNCTION: Part of the Ssh1 complex, which probably is the major component of a channel-forming translocon complex that may function exclusively in the cotranslational pathway of protein endoplasmic reticulum (ER) import. KEYWORDS: 3D-structure;Endoplasmic reticulum;Membrane;Protein transport;Reference proteome;Translocation;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass membrane protein. MISCELLANEOUS: Present with 704 molecules/cell in log phase SD medium." P38361,PROTEIN NAMES: Phosphate permease PHO89 (Na(+)/Pi cotransporter PHO89) PROTEIN FAMILY: Inorganic phosphate transporter (PiT) family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. FUNCTION: Sodium-phosphate symporter. Active in early growth phase. KEYWORDS: Cell membrane;Membrane;Phosphate transport;Reference proteome;Symport;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein P38433,PROTEIN NAMES: Acetylcholinesterase 1 (ACE-1) (AChE 1) PROTEIN FAMILY: Type-B carboxylesterase/lipase family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the type-B carboxylesterase/lipase family. FUNCTION: Rapidly hydrolyzes acetylcholine and releases choline into the synapse (Probable). It can hydrolyze propionylcholine and butyrylthiocholine in vitro (Probable). KEYWORDS: Cell membrane;Disulfide bond;Glycoprotein;Hydrolase;Membrane;Neurotransmitter degradation;Reference proteome;Secreted;Serine esterase;Signal;Synapse SUBCELLULAR LOCATION: Synapse Secreted Cell membrane ; Peripheral membrane protein Note=May be secreted or membrane associated via a non-catalytic subunit. P38439,"PROTEIN NAMES: RNA polymerase-associated protein LEO1 PROTEIN FAMILY: LEO1 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the LEO1 family. FUNCTION: The PAF1 complex is a multifunctional complex. Involved in transcription initiation via genetic interactions with TATA-binding proteins. Involved in elongation. It regulates 3'-end formation of snR47 by modulating the recruitment or stable association of NRD1 and NAB3 with RNA polymerase II. Also has a role in transcription-coupled histone modification. Required for activation of RAD6 ubiquitin conjugate and the BRE1 ubiquitin ligase which ubiquitinate 'Lys-126' histone H2B. Activates the SET1 histone methyltransferase complex for methylation of 'Lys-4' of histone H3 and for methylation of 'Lys-73' of histone H3 by DOT1 and 'Lys-36' of histone H3 by SET2. KEYWORDS: Activator;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus, nucleoplasm MISCELLANEOUS: Present with 1110 molecules/cell in log phase SD medium." P38447,"PROTEIN NAMES: Endonuclease G, mitochondrial (Endo G) PROTEIN FAMILY: DNA/RNA non-specific endonuclease family ORGANISM: Bos taurus (Bovine) SIMILARITY: Belongs to the DNA/RNA non-specific endonuclease family. FUNCTION: Endonuclease that preferentially catalyzes the cleavage of double-stranded 5-hydroxymethylcytosine (5hmC)-modified DNA (By similarity). The 5hmC-modified nucleotide does not increase the binding affinity, but instead increases the efficiency of cutting and specifies the site of cleavage for the modified DNAs (By similarity). Shows significantly higher affinity for four- stranded Holliday junction over duplex and single-stranded DNAs (By similarity). Promotes conservative recombination when the DNA is 5hmC-modified (By similarity). Promotes autophagy through the suppression of mTOR by its phosphorylation-mediated interaction with YWHAG and its endonuclease activity-mediated DNA damage response (By similarity). GSK3-beta mediated phosphorylation of ENDOG enhances its interaction with YWHAG, leading to the release of TSC2 and PIK3C3 from YWHAG resulting in mTOR pathway suppression and autophagy initiation (By similarity). Promotes cleavage of mtDNA in response to oxidative and nitrosative stress, in turn inducing compensatory mtDNA replication (By similarity). KEYWORDS: Direct protein sequencing;Disulfide bond;Endonuclease;Hydrolase;Magnesium;Manganese;Metal-binding;Mitochondrion;Nuclease;Phosphoprotein;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Mitochondrion " P38486,"PROTEIN NAMES: Galectin-3 (Gal-3) (35 kDa lectin) (Carbohydrate-binding protein 35) (CBP 35) (Galactose-specific lectin 3) (IgE-binding protein) (Laminin-binding protein) (Lectin L-29) (Mac-2 antigen) ORGANISM: Canis lupus familiaris (Dog) (Canis familiaris) FUNCTION: Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. Together with TRIM16, coordinates the recognition of membrane damage with mobilization of the core autophagy regulators ATG16L1 and BECN1 in response to damaged endomembranes (By similarity). KEYWORDS: Acetylation;Cytoplasm;Differentiation;Direct protein sequencing;Disulfide bond;IgE-binding protein;Immunity;Innate immunity;Lectin;mRNA processing;mRNA splicing;Nucleus;Phosphoprotein;Reference proteome;Repeat;Secreted;Spliceosome SUBCELLULAR LOCATION: Cytoplasm Nucleus Secreted Note=Secreted by a non-classical secretory pathway and associates with the cell surface. Can be secreted; the secretion is dependent on protein unfolding and facilitated by the cargo receptor TMED10; it results in protein translocation from the cytoplasm into the ERGIC (endoplasmic reticulum-Golgi intermediate compartment) followed by vesicle entry and secretion. " P38590,"PROTEIN NAMES: Tyrosine-protein phosphatase MSG5 PROTEIN FAMILY: Protein-tyrosine phosphatase family, Non-receptor class dual specificity subfamily ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily. FUNCTION: Dual specificity phosphatase that dephosphorylates MAP kinase FUS3 on both a Tyr and a Ser or Thr. Has a role in adaptation to pheromone. KEYWORDS: Cell cycle;Hydrolase;Phosphoprotein;Protein phosphatase;Reference proteome MISCELLANEOUS: Present with 538 molecules/cell in log phase SD medium." P38604,"PROTEIN NAMES: Lanosterol synthase ERG7 (2,3-epoxysqualene--lanosterol cyclase ERG7) (Ergosterol biosynthetic protein 7) (Oxidosqualene--lanosterol cyclase ERG7) (OSC) PROTEIN FAMILY: Terpene cyclase/mutase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the terpene cyclase/mutase family. FUNCTION: Lanosterol synthase; part of the third module of ergosterol biosynthesis pathway that includes the late steps of the pathway. ERG7 catalyzes the cyclization of (S)-2,3 oxidosqualene to lanosterol, a reaction that forms the sterol core. The third module or late pathway involves the ergosterol synthesis itself through consecutive reactions that mainly occur in the endoplasmic reticulum (ER) membrane. Firstly, the squalene synthase ERG9 catalyzes the condensation of 2 farnesyl pyrophosphate moieties to form squalene, which is the precursor of all steroids. Squalene synthase is crucial for balancing the incorporation of farnesyl diphosphate (FPP) into sterol and nonsterol isoprene synthesis. Secondly, the squalene epoxidase ERG1 catalyzes the stereospecific oxidation of squalene to (S)-2,3-epoxysqualene, which is considered to be a rate-limiting enzyme in steroid biosynthesis. Then, the lanosterol synthase ERG7 catalyzes the cyclization of (S)-2,3 oxidosqualene to lanosterol, a reaction that forms the sterol core. In the next steps, lanosterol is transformed to zymosterol through a complex process involving various demethylation, reduction and desaturation reactions. The lanosterol 14-alpha-demethylase ERG11 (also known as CYP51) catalyzes C14-demethylation of lanosterol to produce 4,4'-dimethyl cholesta-8,14,24-triene-3-beta-ol, which is critical for ergosterol biosynthesis. The C-14 reductase ERG24 reduces the C14=C15 double bond of 4,4-dimethyl-cholesta-8,14,24-trienol to produce 4,4-dimethyl-cholesta-8,24-dienol. 4,4-dimethyl-cholesta-8,24-dienol is substrate of the C-4 demethylation complex ERG25-ERG26-ERG27 in which ERG25 catalyzes the three-step monooxygenation required for the demethylation of 4,4-dimethyl and 4alpha-methylsterols, ERG26 catalyzes the oxidative decarboxylation that results in a reduction of the 3-beta-hydroxy group at the C-3 carbon to an oxo group, and ERG27 is responsible for the reduction of the keto group on the C-3. ERG28 has a role as a scaffold to help anchor ERG25, ERG26 and ERG27 to the endoplasmic reticulum and ERG29 regulates the activity of the iron-containing C4-methylsterol oxidase ERG25. Then, the sterol 24-C-methyltransferase ERG6 catalyzes the methyl transfer from S-adenosyl-methionine to the C-24 of zymosterol to form fecosterol. The C-8 sterol isomerase ERG2 catalyzes the reaction which results in unsaturation at C-7 in the B ring of sterols and thus converts fecosterol to episterol. The sterol-C5-desaturase ERG3 then catalyzes the introduction of a C-5 double bond in the B ring to produce 5-dehydroepisterol. The C-22 sterol desaturase ERG5 further converts 5-dehydroepisterol into ergosta-5,7,22,24(28)-tetraen-3beta-ol by forming the C-22(23) double bond in the sterol side chain. Finally, ergosta-5,7,22,24(28)-tetraen-3beta-ol is substrate of the C-24(28) sterol reductase ERG4 to produce ergosterol. PATHWAY: Terpene metabolism; lanosterol biosynthesis; lanosterol from farnesyl diphosphate: step 3/3. KEYWORDS: Acetylation;Direct protein sequencing;Endoplasmic reticulum;Isomerase;Lipid biosynthesis;Lipid droplet;Lipid metabolism;Membrane;Reference proteome;Repeat;Steroid biosynthesis;Steroid metabolism;Sterol biosynthesis;Sterol metabolism SUBCELLULAR LOCATION: Lipid droplet Endoplasmic reticulum membrane ; Peripheral membrane protein Note=Predominantly in lipid particles. MISCELLANEOUS: Present with 2193 molecules/cell in log phase SD medium." P38679,"PROTEIN NAMES: Serine/threonine-protein kinase cot-1 (Colonial temperature-sensitive protein 1) PROTEIN FAMILY: Protein kinase superfamily, STE Ser/Thr protein kinase family, COT1 subfamily ORGANISM: Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) SIMILARITY: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. COT1 subfamily. FUNCTION: Protein kinase required for hyphal elongation. KEYWORDS: Alternative initiation;ATP-binding;Kinase;Nucleotide-binding;Phosphoprotein;Reference proteome;Serine/threonine-protein kinase;Transferase MISCELLANEOUS: [Isoform Long]: Produced preferentially in dark grown mycelia.; MISCELLANEOUS: [Isoform Short]: Produced preferentially in illuminated mycelia." P38695,"PROTEIN NAMES: Probable glucose transporter HXT5 PROTEIN FAMILY: Major facilitator superfamily, Sugar transporter family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. FUNCTION: Probable glucose transporter. KEYWORDS: Glycoprotein;Isopeptide bond;Membrane;Reference proteome;Repeat;Sugar transport;Transmembrane;Transmembrane helix;Transport;Ubl conjugation SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. MISCELLANEOUS: Glucose transport is thought to be mediated by two kinetically distinct systems, a glucose-repressible high-affinity system and a constitutive low-affinity system." P38700,"PROTEIN NAMES: Adaptin medium chain homolog APM2 (Adaptin-mu1-II) PROTEIN FAMILY: Adaptor complexes medium subunit family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the adaptor complexes medium subunit family. FUNCTION: Component of the AP-1-related (AP-1R) complex, an adapter protein complex that mediates of cargo protein sorting in clathrin-coated vesicles. AP-1R has a specific role in SNARE SNC1 sorting. In contrast to the APM1-containing AP-1 complex, AP-1R is incapable of sorting CHS3. KEYWORDS: Cytoplasmic vesicle;Endosome;Golgi apparatus;Membrane;Protein transport;Reference proteome;Transport SUBCELLULAR LOCATION: Golgi apparatus membrane ; Peripheral membrane protein Early endosome membrane ; Peripheral membrane protein Cytoplasmic vesicle, clathrin-coated vesicle membrane ; Peripheral membrane protein MISCELLANEOUS: Present with 2890 molecules/cell in log phase SD medium." P38704,"PROTEIN NAMES: Transcription factor STP2 ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Transcription factor involved in the regulation of gene expression in response to extracellular amino acid levels. Synthesized as latent cytoplasmic precursor, which, upon a signal initiated by the plasma membrane SPS (SSY1-PTR3-SSY5) amino acid sensor system, becomes proteolytically activated and relocates to the nucleus, where it induces the expression of SPS-sensor-regulated genes, including the amino-acid permeases BAP2 and BAP3. Binding to promoters is facilitated by DAL81 (By similarity). Involved in the repression of genes subject to nitrogen catabolite repression and genes involved in stress response. Negatively regulated by inner nuclear membrane proteins ASI1, ASI2 and ASI3, which prevent unprocessed precursor forms that escape cytoplasmic anchoring from inducing SPS-sensor-regulated genes. KEYWORDS: Cell membrane;DNA-binding;Membrane;Metal-binding;Nucleus;Reference proteome;Repeat;Zinc;Zinc-finger;Zymogen SUBCELLULAR LOCATION: Cell membrane ; Peripheral membrane protein ; Cytoplasmic side Nucleus Note=Localizes to the cytoplasm in its unprocessed form and is targeted to the nucleus after proteolytic processing upon induction by amino acids. MISCELLANEOUS: Present with 6490 molecules/cell in log phase SD medium." P38758,PROTEIN NAMES: Putative oxidoreductase TDA3 (Batten disease protein 3) (Topoisomerase I damage affected protein 3) PROTEIN FAMILY: TDA3 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the TDA3 family. FUNCTION: Putative oxidoreductase that negatively regulates the retrieval of cargo from late endosomes to the Golgi. Regulates YIF1 and KEX2 localization. Required for fast DNA replication. KEYWORDS: Cytoplasm;Endosome;Oxidoreductase;Phosphoprotein;Reference proteome;Transport SUBCELLULAR LOCATION: Cytoplasm. Late endosome. Note=The recruitment from the cytoplasm to endosomal structures is facilitated by BTN2. MISCELLANEOUS: Present with 5240 molecules/cell in log phase SD medium. P38759,PROTEIN NAMES: Vacuolar protein sorting-associated protein 29 (Carboxypeptidase Y-deficient protein 11) (Vesicle protein sorting 29) PROTEIN FAMILY: VPS29 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the VPS29 family. FUNCTION: Plays a role in vesicular protein sorting. Required for the endosome-to-Golgi retrieval of the vacuolar protein sorting receptor VPS10. Component of the membrane-associated retromer complex which is essential in endosome-to-Golgi retrograde transport. The VPS29-VPS26-VPS35 subcomplex may be involved in cargo selection. KEYWORDS: Protein transport;Reference proteome;Transport MISCELLANEOUS: Present with 259 molecules/cell in log phase SD medium. P38764,PROTEIN NAMES: 26S proteasome regulatory subunit RPN1 (HMG-CoA reductase degradation protein 2) (Proteasome non-ATPase subunit 1) PROTEIN FAMILY: Proteasome subunit S2 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the proteasome subunit S2 family. FUNCTION: Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins. KEYWORDS: 3D-structure;Direct protein sequencing;Phosphoprotein;Proteasome;Reference proteome;Repeat MISCELLANEOUS: Present with 306000 molecules/cell in log phase SD medium. P38766,"PROTEIN NAMES: ATP-dependent DNA helicase RRM3 (Regulation of Ty1 transposition protein 104) (rDNA recombination mutation protein 3) PROTEIN FAMILY: Helicase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the helicase family. FUNCTION: 5' to 3' DNA replicative helicase recruited to paused replisomes to promote fork progression throughout nonhistone protein-DNA complexes, naturally occurring impediments that are encountered in each S phase where replication forks pauses. Needed for normal fork progression through over 1000 discrete sites scattered throughout the genome, like rDNA, tRNA genes, centromeres, active replication origins, or transcriptional silencers. Required for timely replication of the telomere and subtelomeric DNA and for wild-type levels of telomeric silencing. Involved in regulation of Ty1 transposition and protects the genome from instability at nascent sites of retrotransposition. Involved in DNA repair during stalled replication fork, regulation of fragile sites expression and essential for genome stability. Also plays a role in mtDNA replication. Has G-quadruplex (G4) unwinding activity and can suppress G4-induced genome instability when PIF1 levels are low. KEYWORDS: ATP-binding;Chromosome;DNA damage;DNA repair;DNA-binding;Helicase;Hydrolase;Nucleotide-binding;Nucleus;Phosphoprotein;Reference proteome;Telomere SUBCELLULAR LOCATION: Nucleus. Chromosome, telomere. MISCELLANEOUS: Present with 656 molecules/cell in log phase SD medium." P38770,PROTEIN NAMES: Nucleus export protein BRL1 (BRR6-like protein 1) PROTEIN FAMILY: BRL1/BRR6 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the BRL1/BRR6 family. FUNCTION: Involved in mRNA and protein export from nucleus. KEYWORDS: Membrane;mRNA transport;Nucleus;Protein transport;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Nucleus membrane ; Multi-pass membrane protein MISCELLANEOUS: Present with 1280 molecules/cell in log phase SD medium. P38779,"PROTEIN NAMES: Proteasome-interacting protein CIC1 (Core interacting component 1) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: An adapter protein that specifically links the 26S proteasome to its substrate CDC4 which is one of the substrate recognition subunits of the SCF E3 ubiquitin ligase complex. Required for turnover of cell cycle regulatory proteins CDC4 and GRR1. Required for synthesis and nuclear export of 60S ribosomal subunits. Required for vegetative growth. KEYWORDS: 3D-structure;Nucleus;Proteasome;Reference proteome;Ribosome biogenesis SUBCELLULAR LOCATION: Nucleus, nucleolus MISCELLANEOUS: Present with 45900 molecules/cell in log phase SD medium." P38788,"PROTEIN NAMES: Ribosome-associated complex subunit SSZ1 (DnaK-related protein SSZ1) (Heat shock protein 70 homolog SSZ1) (Pleiotropic drug resistance protein 13) PROTEIN FAMILY: Heat shock protein 70 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the heat shock protein 70 family. FUNCTION: Component of the ribosome-associated complex (RAC), a heterodimeric chaperone complex involved in regulation of accurate translation termination and in folding or maintaining nascent polypeptides in a folding-competent state. RAC stimulates the ATPase activity of the ribosome-associated pool of Hsp70-type chaperones SSB1/SSB2 that bind to the nascent polypeptide chain. SSZ1 is required for ZUO1 to function efficiently as a J-protein for SSB1/SSB2. Also involved in pleiotropic drug resistance by post-translational activation of transcription factor PDR1. KEYWORDS: 3D-structure;ATP-binding;Cytoplasm;Nucleotide-binding;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cytoplasm MISCELLANEOUS: Present with 73600 molecules/cell in log phase SD medium." P38792,"PROTEIN NAMES: Exosome complex component RRP4 (Ribosomal RNA-processing protein 4) PROTEIN FAMILY: RRP4 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the RRP4 family. FUNCTION: Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and cryptic unstable transcripts (CUTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and in RNA surveillance pathways, preventing translation of aberrant mRNAs. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. RRP4 as peripheral part of the Exo-9 complex is thought to stabilize the hexameric ring of RNase PH-domain subunits. KEYWORDS: 3D-structure;Acetylation;Cytoplasm;Direct protein sequencing;Exosome;Nucleus;Phosphoprotein;Reference proteome;RNA-binding;rRNA processing SUBCELLULAR LOCATION: Cytoplasm. Nucleus, nucleolus. MISCELLANEOUS: Present with 4840 molecules/cell in log phase SD medium." P38797,"PROTEIN NAMES: Protein phosphatase 2C homolog 7, mitochondrial (PP2C-7) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Protein phosphatase which positively regulates biosynthesis of the ubiquinone, coenzyme Q. Dephosphorylates and activates the ubiquinone biosynthesis protein CAT5/COQ7. Also dephosphorylates CIT1 on 'Ser-462', which leads to its activation. KEYWORDS: Hydrolase;Magnesium;Manganese;Metal-binding;Mitochondrion;Protein phosphatase;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Mitochondrion " P38806,PROTEIN NAMES: Chromatin modification-related protein YNG2 (ESA1-associated factor 4) (ING1 homolog 2) PROTEIN FAMILY: ING family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the ING family. FUNCTION: Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of selected genes principally by acetylation of nucleosomal histone H4 and H2A. The NuA4 complex is also involved in DNA repair. Involved in cell cycle progression and meiosis. KEYWORDS: 3D-structure;Cell cycle;Chromatin regulator;Coiled coil;Direct protein sequencing;DNA damage;DNA repair;Meiosis;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus P38817,"PROTEIN NAMES: ADP-ribosylation factor-binding protein GGA2 (Golgi-localized, gamma ear-containing, ARF-binding protein 2) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: May play a role in the regulation of membrane traffic through the trans-Golgi network. KEYWORDS: 3D-structure;Coiled coil;Golgi apparatus;Isopeptide bond;Protein transport;Reference proteome;Transport;Ubl conjugation SUBCELLULAR LOCATION: Golgi apparatus, trans-Golgi network. MISCELLANEOUS: Present with 2270 molecules/cell in log phase SD medium." P38825,PROTEIN NAMES: Protein TOM71 (71 kDa mitochondrial outer membrane protein) PROTEIN FAMILY: Tom70 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the Tom70 family. FUNCTION: Involved in MBF1-mediated mitochondrial morphogenesis. KEYWORDS: 3D-structure;Membrane;Mitochondrion;Mitochondrion outer membrane;Phosphoprotein;Reference proteome;Repeat;TPR repeat;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Mitochondrion outer membrane ; Single-pass membrane protein MISCELLANEOUS: Present with 4110 molecules/cell in log phase SD medium. P38830,"PROTEIN NAMES: Meiosis-specific transcription factor NDT80 ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Transcription factor required for successful completion of meiosis and spore formation. Gets activated after completion of meiotic recombination at the end of prophase I. Recognizes and binds to the middle sporulation element (MSE) 5'-C[AG]CAAA[AT]-3' in the promoter region of stage-specific genes that are required for progression through meiosis and sporulation. Competes for binding to MSE with the transcriptional repressor SUM1, which represses middle sporulation-specific genes during mitosis and early sporulation. KEYWORDS: 3D-structure;Cell cycle;Cell division;DNA-binding;Meiosis;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " P38840,"PROTEIN NAMES: Aromatic amino acid aminotransferase 2 (Aromatic amino acid aminotransferase II) (Aromatic amino acid-requiring protein 9) (Kynurenine aminotransferase I) (KAT I) PROTEIN FAMILY: Class-I pyridoxal-phosphate-dependent aminotransferase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. FUNCTION: General aromatic amino acid transaminase involved in several otherwise unrelated metabolic pathways. Mainly involved in tryptophan degradation. Active with phenylalanine, tyrosine and tryptophan as amino donors and with phenylpyruvate, hydroxyphenylpyruvate and pyruvate as amino acceptors. Does not accept glutamate or 2-oxoglutarate as substrates. Also active with methionine, leucine, glutamine and kynurenine. Catalyzes the formation of methionine from 2-keto-4-methylthiobutyrate (KMTB) in the methionine salvage pathway primarily using aromatic amino acids (tyrosine, phenylalanine and tryptophan) as the amino donors. Catalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid (KA) with pyruvate as amino acceptor. PATHWAY: Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 6/6.; PATHWAY: Amino-acid degradation; L-kynurenine degradation; kynurenate from L-kynurenine: step 1/2. KEYWORDS: Aminotransferase;Cytoplasm;Phosphoprotein;Pyridoxal phosphate;Reference proteome;Transferase SUBCELLULAR LOCATION: Cytoplasm MISCELLANEOUS: Present with 7700 molecules/cell in log phase SD medium." P38863,"PROTEIN NAMES: Spindle pole body component SPC97 PROTEIN FAMILY: TUBGCP family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the TUBGCP family. FUNCTION: Involved in microtubule organization by the microtubule organizing center, the spindle pole body (SPB). Probably part of the microtubule attachment site at the SPB. KEYWORDS: 3D-structure;Cytoplasm;Cytoskeleton;Microtubule;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body. MISCELLANEOUS: Present with 2230 molecules/cell in log phase SD medium." P38869,"PROTEIN NAMES: Protein SVP26 (Sed5 compartment vesicle protein of 26 kDa) PROTEIN FAMILY: SVP26 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the SVP26 family. FUNCTION: Plays a role in retention of a subset of membrane proteins in the early Golgi compartments. Facilitates endoplasmic reticulum to Golgi transport of mannosyltransferases MNN2 and MNN5. KEYWORDS: Glycoprotein;Golgi apparatus;Membrane;Protein transport;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Golgi apparatus, cis-Golgi network membrane ; Multi-pass membrane protein Note=Early Golgi. MISCELLANEOUS: Present with 1810 molecules/cell in log phase SD medium." P38882,"PROTEIN NAMES: U3 small nucleolar RNA-associated protein 9 (U3 snoRNA-associated protein 9) (U three protein 9) (U3 protein 9 required for transcription) (t-UTP9) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Involved in nucleolar processing of pre-18S ribosomal RNA. Required for optimal pre-ribosomal RNA transcription by RNA polymerase I together with a subset of U3 proteins required for transcription (t-UTPs). KEYWORDS: 3D-structure;Nucleus;Phosphoprotein;Reference proteome;Ribonucleoprotein;Ribosome biogenesis;rRNA processing;Transcription SUBCELLULAR LOCATION: Nucleus, nucleolus Note=Associated with ribosomal chromatin, even in the absence of transcription. MISCELLANEOUS: Present with 20000 molecules/cell in log phase SD medium." P38907,"PROTEIN NAMES: Inner kinetochore subunit CHL4 (CENP-N homolog) (Chromosome loss protein 4) (Chromosome transmission fidelity protein 17) (Constitutive centromere-associated network protein CHL4) (Minichromosome maintenance protein 17) PROTEIN FAMILY: CENP-N/CHL4 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the CENP-N/CHL4 family. FUNCTION: Component of the kinetochore, a multiprotein complex that assembles on centromeric DNA and attaches chromosomes to spindle microtubules, mediating chromosome segregation and sister chromatid segregation during meiosis and mitosis. Component of the inner kinetochore constitutive centromere-associated network (CCAN), which serves as a structural platform for outer kinetochore assembly. KEYWORDS: 3D-structure;Cell cycle;Cell division;Centromere;Chromosome;Kinetochore;Meiosis;Mitosis;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus Chromosome, centromere, kinetochore Note=Associated with kinetochores. MISCELLANEOUS: Present with 606 molecules/cell in log phase SD medium." P38920,"PROTEIN NAMES: DNA mismatch repair protein MLH1 (MutL protein homolog 1) (Post meiotic segregation protein 2) PROTEIN FAMILY: DNA mismatch repair MutL/HexB family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the DNA mismatch repair MutL/HexB family. FUNCTION: Required for DNA mismatch repair (MMR), correcting base-base mismatches and insertion-deletion loops (IDLs) resulting from DNA replication, DNA damage or from recombination events between non-identical sequences during meiosis. Component of different MutL heterodimers that form a ternary complex with the MutS heterodimers, which initially recognize the DNA mismatches. This complex is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. Plays a major role in maintaining the genetic stability of simple sequence repeats, the repair of heteroduplex sites present in meiotic recombination intermediates, and the promotion of meiotic crossing-over. KEYWORDS: 3D-structure;DNA damage;DNA repair;Nucleus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: Present with 319 molecules/cell in log phase SD medium." P38922,"PROTEIN NAMES: Serine/arginine (SR)-type shuttling mRNA binding protein HRB1 (Hypothetical RNA-binding protein 1) (Polyadenylate-binding protein HRB1) (Protein TOM34) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Binds to intron-containing transcripts and is involved in quality control for the export of spliced mRNAs from the nucleus. Binds to pre-mRNAs until splicing is completed or until faulty mRNAs are degraded. On correctly spliced mRNAs, GBP2 and HRB1 recruit MEX67 to allow nuclear export. On faulty mRNAs, GBP2 and HRB1 associate with the TRAMP complex that guides those pre-mRNAs to the exosome for degradation. KEYWORDS: 3D-structure;Cytoplasm;Methylation;Nucleus;Phosphoprotein;Reference proteome;Repeat;RNA-binding SUBCELLULAR LOCATION: Cytoplasm Nucleus Nucleus Cytoplasm, P-body Cytoplasm, Stress granule Note=Shuttles between nucleus and cytoplasm. Nuclear at steady state and its import is mediated by the karyopherin MTR10. Export is dependent on active transcription and the export of mRNAs in general. Depends on MFT1 and HPR1 for nuclear export. MISCELLANEOUS: Present with 1990 molecules/cell in log phase SD medium." P38925,"PROTEIN NAMES: Manganese transporter SMF1 PROTEIN FAMILY: NRAMP family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the NRAMP family. FUNCTION: High-affinity manganese transporter involved in manganese uptake from the extracellular environment. Contributes also to cellular accumulation of other divalent metal ions such as cadmium, cobalt, copper, iron and nickel. KEYWORDS: Cell membrane;Isopeptide bond;Manganese;Membrane;Metal-binding;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix;Transport;Ubl conjugation SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Note=In presence of excess manganese, targeted to the vacuolar lumen, where it is degraded. " P38934,"PROTEIN NAMES: Nuclear segregation protein BFR1 (Brefeldin A resistance protein 1) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Implicated in secretion, nuclear segregation and in maintenance of cell size. KEYWORDS: Coiled coil;Phosphoprotein;Reference proteome MISCELLANEOUS: Present with 33400 molecules/cell in log phase SD medium." P38935,"PROTEIN NAMES: DNA-binding protein SMUBP-2 (ATP-dependent helicase IGHMBP2) (Glial factor 1) (GF-1) (Immunoglobulin mu-binding protein 2) PROTEIN FAMILY: DNA2/NAM7 helicase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the DNA2/NAM7 helicase family. FUNCTION: 5' to 3' helicase that unwinds RNA and DNA duplexes in an ATP-dependent reaction. Specific to 5'-phosphorylated single-stranded guanine-rich sequences. May play a role in RNA metabolism, ribosome biogenesis or initiation of translation. May play a role in regulation of transcription (By similarity). Interacts with tRNA-Tyr. KEYWORDS: 3D-structure;Acetylation;Activator;ATP-binding;Cell projection;Charcot-Marie-Tooth disease;Cytoplasm;Direct protein sequencing;Disease variant;DNA-binding;Helicase;Hydrolase;Metal-binding;Neurodegeneration;Neuropathy;Nucleotide-binding;Nucleus;Reference proteome;Ribonucleoprotein;RNA-binding;Transcription;Transcription regulation;tRNA-binding;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Cytoplasm Cell projection, axon " P38937,"PROTEIN NAMES: Tethering factor for nuclear proteasome cut8 (Cell untimely torn protein 8) PROTEIN FAMILY: Cut8/STS1 family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the cut8/STS1 family. FUNCTION: Together with nucleoporin alm1, tethers the proteasome to the nuclear envelope. Involved in ubiquitin-mediated protein degradation and facilitates the degradation of nuclear proteins like mitotic cyclin and cut2. Required for normal progression of anaphase. KEYWORDS: 3D-structure;Nucleus;Reference proteome;Ubl conjugation SUBCELLULAR LOCATION: Nucleus envelope " P38954,"PROTEIN NAMES: Inositolphosphotransferase 1 (Mannosyl diphosphorylinositol ceramide synthase) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Catalyzes the addition of a phosphorylinositol group onto mannosyl phosphorylinositol ceramide (MIPC) to form mannosyl diphosphorylinositol ceramide (M(IP)2C), the major sphingolipid in membranes of S.cerevisiae. KEYWORDS: Golgi apparatus;Membrane;Reference proteome;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus membrane ; Multi-pass membrane protein MISCELLANEOUS: Present with 606 molecules/cell in log phase SD medium." P38959,PROTEIN NAMES: Vacuolar protein sorting-associated protein 41 (Vacuolar morphogenesis protein 2) PROTEIN FAMILY: VPS41 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the VPS41 family. FUNCTION: Required for vacuolar assembly and vacuolar traffic. Acts as component of the HOPS complex that acts during the docking stage of vacuole fusion. HOPS is an effector for the vacuolar Rab GTPase YPT7 and is required for vacuolar SNARE complex assembly. It remains bound to SNARE complexes after vacuole fusion. KEYWORDS: 3D-structure;Phosphoprotein;Protein transport;Reference proteome;Repeat;Transport;Vacuole;WD repeat SUBCELLULAR LOCATION: Vacuole MISCELLANEOUS: Present with 1170 molecules/cell in log phase SD medium. P38960,"PROTEIN NAMES: Protein STN1 ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Has a role in telomere length regulation and telomere end protection. Acts as an inhibitor of telomerase loading through its interaction with CDC13. KEYWORDS: 3D-structure;Chromosome;Reference proteome;Telomere SUBCELLULAR LOCATION: Chromosome, telomere " P38992,PROTEIN NAMES: Sphingolipid C4-hydroxylase SUR2 (Syringomycin response protein 2) PROTEIN FAMILY: Sterol desaturase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the sterol desaturase family. FUNCTION: Required for hydroxylation of C-4 in the sphingoid moiety of ceramide. Catalyzes the conversion of sphinganine to phytosphingosine in sphingolipid biosynthesis. Involved in the response to syringomycin. PATHWAY: Membrane lipid metabolism; sphingolipid biosynthesis. KEYWORDS: Endoplasmic reticulum;Lipid biosynthesis;Lipid metabolism;Membrane;Oxidoreductase;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein MISCELLANEOUS: Present with 54300 molecules/cell in log phase SD medium. P38994,"PROTEIN NAMES: Phosphatidylinositol 4-phosphate 5-kinase MSS4 (1-phosphatidylinositol 4-phosphate kinase) (Diphosphoinositide kinase) (PIP5K) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Catalyzes the phosphorylation of phosphatidylinositol 4-phosphate on the fifth hydroxyl of the myo-inositol ring, to form phosphatidylinositol 4,5-bisphosphate. Acts downstream of STT4, but in a pathway that does not involve PKC1. May be involved in the organization of the actin cytoskeleton. KEYWORDS: ATP-binding;Kinase;Nucleotide-binding;Phosphoprotein;Reference proteome;Transferase " P38996,"PROTEIN NAMES: Nuclear polyadenylated RNA-binding protein 3 ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: May be required for packaging pre-mRNAs into ribonucleoprotein structures amenable to efficient nuclear RNA processing. Binds to poly(A)+ RNA. Appears to act in the maintenance of CLN3 mRNA levels. KEYWORDS: 3D-structure;Nucleus;Phosphoprotein;Reference proteome;RNA-binding SUBCELLULAR LOCATION: Nucleus, nucleoplasm MISCELLANEOUS: Present with 5830 molecules/cell in log phase SD medium." P39006,"PROTEIN NAMES: Phosphatidylserine decarboxylase proenzyme 1, mitochondrial [Cleaved into: Phosphatidylserine decarboxylase 1 beta chain; Phosphatidylserine decarboxylase 1 alpha chain] PROTEIN FAMILY: Phosphatidylserine decarboxylase family, PSD-B subfamily, Eukaryotic type I sub-subfamily ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the phosphatidylserine decarboxylase family. PSD-B subfamily. Eukaryotic type I sub-subfamily. FUNCTION: Catalyzes the formation of phosphatidylethanolamine (PtdEtn) from phosphatidylserine (PtdSer). Plays a central role in phospholipid metabolism and in the interorganelle trafficking of phosphatidylserine. Phosphatidylethanolamine formed in the mitochondria is exported to other membranes to fullfill their requirements for PtdEtn. Required for normal mitochondrial morphology and proper mitochondrial fusion during yeast mating. Involved in lipid droplet biogenesis at the endoplasmic reticulum membrane. Required for induction of mitophagy during nitrogen starvation. Appears to play a specific role in supporting respiratory complex III activity. PATHWAY: Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from CDP-diacylglycerol: step 2/2. KEYWORDS: Decarboxylase;Endoplasmic reticulum;Glycoprotein;Lipid biosynthesis;Lipid droplet;Lipid metabolism;Lyase;Membrane;Mitochondrion;Mitochondrion inner membrane;Phospholipid biosynthesis;Phospholipid metabolism;Pyruvate;Reference proteome;Transit peptide;Transmembrane;Transmembrane helix;Zymogen SUBCELLULAR LOCATION: [Phosphatidylserine decarboxylase 1 beta chain]: Mitochondrion inner membrane ; Single-pass membrane protein ; Intermembrane side.; SUBCELLULAR LOCATION: [Phosphatidylserine decarboxylase 1 alpha chain]: Mitochondrion inner membrane ; Peripheral membrane protein ; Intermembrane side Note=Anchored to the mitochondrial inner membrane through its interaction with the integral membrane beta chain.; SUBCELLULAR LOCATION: Lipid droplet Endoplasmic reticulum membrane Note=Localizes to sites of lipid droplet formation on the endoplasmic reticulum membrane. MISCELLANEOUS: Present with 1080 molecules/cell in log phase SD medium." P39008,"PROTEIN NAMES: Poly(A) ribonuclease POP2 (CCR4-associated factor 1) PROTEIN FAMILY: CAF1 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the CAF1 family. FUNCTION: Acts as a probably catalytic component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover. In vitro, POP2 has 3'-exoribonuclease activity with a preference for poly(A) RNAs, but also degrades poly(U) and poly(C) RNAs. Is part of a glucose-sensing system involved in growth control in response to glucose availability. KEYWORDS: 3D-structure;Acetylation;Activator;Cytoplasm;Exonuclease;Hydrolase;Magnesium;Metal-binding;Nuclease;Nucleus;Phosphoprotein;Reference proteome;Repressor;RNA-binding;Transcription;Transcription regulation SUBCELLULAR LOCATION: Cytoplasm Nucleus MISCELLANEOUS: Present with 1520 molecules/cell in log phase SD medium." P39009,"PROTEIN NAMES: DNA damage response protein kinase DUN1 PROTEIN FAMILY: Protein kinase superfamily, CAMK Ser/Thr protein kinase family, CHEK2 subfamily ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CHEK2 subfamily. FUNCTION: Transducer of the DNA damage signal. Phosphorylates SML1 on serine residues. Cooperates with the PAN deadenylation complex in the regulation of RAD5 mRNA levels and cell survival in response to replicational stress. KEYWORDS: 3D-structure;ATP-binding;DNA damage;Kinase;Nucleotide-binding;Nucleus;Phosphoprotein;Reference proteome;Serine/threonine-protein kinase;Transferase SUBCELLULAR LOCATION: Nucleus. MISCELLANEOUS: Present with 3480 molecules/cell in log phase SD medium." P39010,"PROTEIN NAMES: Palmitoyltransferase AKR1 (Ankyrin repeat-containing protein AKR1) PROTEIN FAMILY: DHHC palmitoyltransferase family, AKR/ZDHHC17 subfamily ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the DHHC palmitoyltransferase family. AKR/ZDHHC17 subfamily. FUNCTION: Palmitoyltransferase specific for casein kinase 1. Palmitoylates isoforms YCK1 and YCK2 at both C-terminal cysteine residues, which is required for their proper plasma membrane localization. Required for constitutive endocytosis of a-factor receptor STE3 and both constitutive and pheromone-induced endocytosis of alpha-factor receptor STE2. KEYWORDS: Acyltransferase;ANK repeat;Endosome;Golgi apparatus;Lipoprotein;Membrane;Palmitate;Phosphoprotein;Reference proteome;Repeat;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Early endosome membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. MISCELLANEOUS: Present with 4072 molecules/cell in log phase SD medium." P39042,PROTEIN NAMES: D-alanyl-D-alanine carboxypeptidase (DD-carboxypeptidase) (DD-peptidase) (Penicillin-binding protein) (PBP) PROTEIN FAMILY: Peptidase S11 family ORGANISM: Streptomyces sp. (strain K15) SIMILARITY: Belongs to the peptidase S11 family. FUNCTION: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors. PATHWAY: Cell wall biogenesis; peptidoglycan biosynthesis. KEYWORDS: 3D-structure;Carboxypeptidase;Cell shape;Cell wall biogenesis/degradation;Direct protein sequencing;Hydrolase;Peptidoglycan synthesis;Protease;Secreted;Signal SUBCELLULAR LOCATION: Secreted P39045,PROTEIN NAMES: D-alanyl-D-alanine carboxypeptidase (DD-carboxypeptidase) (DD-peptidase) (Penicillin-binding protein) (PBP) PROTEIN FAMILY: Peptidase S13 family ORGANISM: Actinomadura sp. (strain R39) SIMILARITY: Belongs to the peptidase S13 family. FUNCTION: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors. PATHWAY: Cell wall biogenesis; peptidoglycan biosynthesis. KEYWORDS: 3D-structure;Antibiotic resistance;Carboxypeptidase;Cell shape;Cell wall biogenesis/degradation;Direct protein sequencing;Hydrolase;Peptidoglycan synthesis;Protease;Secreted;Signal SUBCELLULAR LOCATION: Secreted. P39065,"PROTEIN NAMES: Acetoin utilization protein AcuA (Protein acetyltransferase AcuA) PROTEIN FAMILY: Acetyltransferase family ORGANISM: Bacillus subtilis (strain 168) SIMILARITY: Belongs to the acetyltransferase family. FUNCTION: Part of the acuABC operon, which is possibly involved in the breakdown of acetoin and butanediol. Acts as an acetyltransferase inactivating acetyl-CoA synthetase AcsA via acetylation at a Lys residue. PATHWAY: Ketone degradation; acetoin degradation. KEYWORDS: Acetoin catabolism;Acyltransferase;Reference proteome;Sporulation;Transferase " P39108,"PROTEIN NAMES: Peroxisomal targeting signal 2 receptor (PTS2 receptor) (Peroxin-7) PROTEIN FAMILY: WD repeat peroxin-7 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the WD repeat peroxin-7 family. FUNCTION: Receptor required for the peroxisomal import of proteins containing a C-terminal PTS2-type peroxisomal targeting signal, such as 3-oxoacyl-CoA thiolase. Specifically binds to cargo proteins containing a PTS2 peroxisomal targeting signal in the cytosol. Cargo protein-binding triggers interaction with PEX21 and formation of a ternary complex composed of PEX21 and PEX7 along with PTS2-containing cargo proteins, which is tranlocated into peroxisomes by passing through the PEX13-PEX14 docking complex. KEYWORDS: 3D-structure;Cytoplasm;Peroxisome;Protein transport;Reference proteome;Repeat;Transport;WD repeat SUBCELLULAR LOCATION: Cytoplasm, cytosol Peroxisome matrix MISCELLANEOUS: Present with 589 molecules/cell in log phase SD medium." P39115,"PROTEIN NAMES: Ribosome protection protein VmlR (Multidrug resistance system ATP-binding protein VmlR) PROTEIN FAMILY: ABC transporter superfamily, ABCF family, ARE2 subfamily ORGANISM: Bacillus subtilis (strain 168) SIMILARITY: Belongs to the ABC transporter superfamily. ABCF family. ARE2 subfamily. FUNCTION: Recognizes and binds in the vacant E-site of ribosomes stalled by some peptidyltransferase center (PTC)-targeting antibiotics. Makes contact with the PTC and both ribosomal subunits. Induces conformational changes in the P-site, which allows it to dislodge the antibiotic from its PTC binding site. Binds to ribosomes either directly following translation initation or subsequent to E tRNA release during elongation. Involved in resistance to a narrow spectrum of antibiotics (the streptogramin A antibiotic virginiamycin M, the lincosamide antibiotic lincomycin and the pleuromutilin antibiotic tiamulin). KEYWORDS: 3D-structure;Antibiotic resistance;ATP-binding;Coiled coil;Cytoplasm;Nucleotide-binding;Reference proteome;Repeat;RNA-binding;rRNA-binding;tRNA-binding SUBCELLULAR LOCATION: Cytoplasm Note=Does not stably associate with ribosomes. " P39116,PROTEIN NAMES: Pectate lyase (PL) PROTEIN FAMILY: Polysaccharide lyase 1 family ORGANISM: Bacillus subtilis (strain 168) SIMILARITY: Belongs to the polysaccharide lyase 1 family. FUNCTION: Produces unsaturated products from polygalacturonate. PATHWAY: Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 2/5. KEYWORDS: 3D-structure;Calcium;Direct protein sequencing;Lyase;Metal-binding;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted P39205,"PROTEIN NAMES: Molybdenum cofactor synthesis protein cinnamon [Includes: Molybdopterin adenylyltransferase (MPT adenylyltransferase) (Domain G); Molybdopterin molybdenumtransferase (MPT Mo-transferase) (Domain E)] PROTEIN FAMILY: MoaB/Mog family; MoeA family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: In the N-terminal section; belongs to the MoaB/Mog family.; SIMILARITY: In the C-terminal section; belongs to the MoeA family. FUNCTION: Catalyzes two steps in the biosynthesis of the molybdenum cofactor. In the first step, molybdopterin is adenylated. Subsequently, molybdate is inserted into adenylated molybdopterin and AMP is released. PATHWAY: Cofactor biosynthesis; molybdopterin biosynthesis. KEYWORDS: ATP-binding;Magnesium;Metal-binding;Molybdenum;Molybdenum cofactor biosynthesis;Multifunctional enzyme;Nucleotide-binding;Phosphoprotein;Reference proteome;Transferase " P39277,"PROTEIN NAMES: L-methionine/branched-chain amino acid exporter YjeH PROTEIN FAMILY: Amino acid-polyamine-organocation (APC) superfamily, Amino acid efflux (AAE) family ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the amino acid-polyamine-organocation (APC) superfamily. Amino acid efflux (AAE) (TC 2.A.3.13) family. FUNCTION: Catalyzes the efflux of L-methionine, L-leucine, L-isoleucine and L-valine. Activity is dependent on electrochemical potential. KEYWORDS: Amino-acid transport;Antiport;Cell inner membrane;Cell membrane;Membrane;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell inner membrane ; Multi-pass membrane protein " P39396,"PROTEIN NAMES: Pyruvate/proton symporter BtsT (Brenztraubensaure transporter) (Pyruvate/H(+) symporter) PROTEIN FAMILY: Peptide transporter carbon starvation (CstA) family ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the peptide transporter carbon starvation (CstA) (TC 2.A.114) family. FUNCTION: Transports pyruvate with a high affinity and specificity. The process is driven by the proton motive force. Under nutrient limiting conditions, mediates the uptake of pyruvate, thus enabling it to be used as a carbon source for the growth and survival. Part of a nutrient-sensing regulatory network composed of the two-component regulatory systems BtsS/BtsR and YpdA/YpdB, and their respective target proteins, BtsT and YhjX. KEYWORDS: Cell inner membrane;Cell membrane;Membrane;Reference proteome;Symport;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell inner membrane ; Multi-pass membrane protein " P39405,"PROTEIN NAMES: Ferric siderophore reductase (Ferric iron reductase protein FhuF) ORGANISM: Escherichia coli (strain K12) FUNCTION: Siderophore-iron reductase which is involved in iron removal from the hydroxamate-type siderophores coprogen, ferrichrome and ferrioxamine B after their transport into the cell. Binds both the iron-loaded and the apo forms of ferrichrome. KEYWORDS: 2Fe-2S;3D-structure;Cell inner membrane;Cell membrane;Cytoplasm;Iron;Iron-sulfur;Membrane;Metal-binding;Oxidoreductase;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Cell inner membrane ; Peripheral membrane protein Note=Loosely associated with the cytoplasmic membrane. " P39453,"PROTEIN NAMES: Sensor protein TorS ORGANISM: Escherichia coli (strain K12) FUNCTION: Member of the two-component regulatory system TorS/TorR involved in the anaerobic utilization of trimethylamine-N-oxide (TMAO). Detects the presence of TMAO in the medium and, in response, activates TorR via a four-step phosphorelay. When TMAO is removed, TorS can dephosphorylate TorR, probably by a reverse phosphorelay involving His-860 and Asp-733. KEYWORDS: 3D-structure;ATP-binding;Cell inner membrane;Cell membrane;Kinase;Membrane;Nucleotide-binding;Phosphoprotein;Reference proteome;Transferase;Transmembrane;Transmembrane helix;Two-component regulatory system SUBCELLULAR LOCATION: Cell inner membrane ; Multi-pass membrane protein " P39540,"PROTEIN NAMES: Fatty acid elongase 1 (3-keto acyl-CoA synthase ELO1) (Elongation of fatty acids protein 1) (Very-long-chain 3-oxoacyl-CoA synthase 1) PROTEIN FAMILY: ELO family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the ELO family. FUNCTION: Component of a microsomal membrane bound medium-chain fatty acid elongation system, which extends medium-chain-length fatty acids to long-chain fatty acids. Component of elongase I, which extends 12-16-carbon fatty acyl-CoAs such as lauroyl-CoA to 14-18-carbon fatty acids by incorporation of malonyl-CoA. KEYWORDS: Endoplasmic reticulum;Fatty acid biosynthesis;Fatty acid metabolism;Lipid biosynthesis;Lipid metabolism;Membrane;Reference proteome;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein MISCELLANEOUS: Present with 937 molecules/cell in log phase SD medium." P39644,"PROTEIN NAMES: NADPH-dependent reductase BacG (Bacilysin biosynthesis oxidoreductase YwfH) (H2HPP reductase) PROTEIN FAMILY: Short-chain dehydrogenases/reductases (SDR) family ORGANISM: Bacillus subtilis (strain 168) SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FUNCTION: Along with the bacABCDEF operon, BacG is involved in the biosynthesis of the nonribosomally synthesized dipeptide antibiotic bacilysin, composed of L-alanine and L-anticapsin. Bacilysin is an irreversible inactivator of the glutaminase domain of glucosamine synthetase. BacG catalyzes the stereoselective reduction of exocyclic-delta(3),delta(5)-dihydro-hydroxyphenylpyruvate (ex-H2HPP), adding a pro-S hydride equivalent to C4 position to yield tetrahydro-hydroxyphenylpyruvate (H4HPP). Although the 3Z,7R-ex-H2HPP isomer is kinetically disfavored by BacB and produced in a smaller quantity than 3E,7R-ex-H2HPP, it is the preferred substrate for the conjugate reduction reaction of BacG. PATHWAY: Antibiotic biosynthesis; bacilysin biosynthesis. KEYWORDS: 3D-structure;Antibiotic biosynthesis;Cytoplasm;NADP;Oxidoreductase;Reference proteome SUBCELLULAR LOCATION: Cytoplasm " P39704,PROTEIN NAMES: Protein ERP2 PROTEIN FAMILY: EMP24/GP25L family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the EMP24/GP25L family. FUNCTION: Involved in vesicular protein trafficking. KEYWORDS: Endoplasmic reticulum;ER-Golgi transport;Membrane;Protein transport;Reference proteome;Signal;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Single-pass type I membrane protein MISCELLANEOUS: Present with 26300 molecules/cell in log phase SD medium. P39707,"PROTEIN NAMES: tRNA-splicing endonuclease subunit SEN34 (Splicing endonuclease of 34 kDa) (tRNA-intron endonuclease SEN34) PROTEIN FAMILY: TRNA-intron endonuclease family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the tRNA-intron endonuclease family. FUNCTION: Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. It probably carries the active site for 3'-splice site cleavage. KEYWORDS: Direct protein sequencing;Lyase;Membrane;Mitochondrion;Mitochondrion outer membrane;Nucleus;Reference proteome;tRNA processing SUBCELLULAR LOCATION: Nucleus Endomembrane system ; Peripheral membrane protein Mitochondrion outer membrane ; Peripheral membrane protein ; Cytoplasmic side Note=The tRNA splicing endonuclease complex is predominantly associated with the outer membrane of mitochondria, suggesting that tRNA splicing mainly takes place on the mitochondrial surface. MISCELLANEOUS: The tRNA splicing endonuclease complex is present with 100 molecules/cell." P39727,"PROTEIN NAMES: ER-derived vesicles protein ERV46 PROTEIN FAMILY: ERGIC family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the ERGIC family. FUNCTION: Constituent of COPII-coated endoplasmic reticulum-derived transport vesicles. Required for efficient transport of a subset of secretory proteins to the Golgi. The C-terminal Phe-Tyr motif is required for exit from the endoplasmic reticulum. Facilitates retrograde transport from the Golgi to the endoplasmic reticulum. KEYWORDS: Endoplasmic reticulum;ER-Golgi transport;Golgi apparatus;Membrane;Protein transport;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Note=Recycles between endoplasmic reticulum and Golgi. Resides in the endoplasmic and Golgi compartments, and then packaged into endoplasmic reticulum derived vesicles. " P39743,"PROTEIN NAMES: Reduced viability upon starvation protein 167 ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Component of a cytoskeletal structure that is required for the formation of endocytic vesicles at the plasma membrane level. Could be implicated in cytoskeletal reorganization in response to environmental stresses and could act in the budding site selection mechanism. KEYWORDS: Acetylation;Actin-binding;Coiled coil;Cytoplasm;Cytoskeleton;Isopeptide bond;Phosphoprotein;Reference proteome;SH3 domain;Ubl conjugation SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton. MISCELLANEOUS: Present with 14600 molecules/cell in log phase SD medium." P39744,"PROTEIN NAMES: Nucleolar complex protein 2 PROTEIN FAMILY: NOC2 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the NOC2 family. FUNCTION: Involved in the intranuclear transport of ribosomal precursors. KEYWORDS: 3D-structure;Nucleus;Phosphoprotein;Reference proteome;Ribosome biogenesis SUBCELLULAR LOCATION: Nucleus, nucleolus MISCELLANEOUS: Present with 29000 molecules/cell in log phase SD medium." P39844,"PROTEIN NAMES: D-alanyl-D-alanine carboxypeptidase DacC (DD-carboxypeptidase) (DD-peptidase) (Penicillin-binding protein 4a) (PBP-4a) PROTEIN FAMILY: Peptidase S13 family ORGANISM: Bacillus subtilis (strain 168) SIMILARITY: Belongs to the peptidase S13 family. FUNCTION: Catalyzes DD-carboxypeptidase and transpeptidation reactions. KEYWORDS: 3D-structure;Cell cycle;Cell division;Cell shape;Cell wall biogenesis/degradation;Direct protein sequencing;Hydrolase;Membrane;Peptidoglycan synthesis;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted Membrane raft Note=May be anchored in the membrane via a C-terminal amphipathic alpha helix. Present in detergent-resistant membrane (DRM) fractions that may be equivalent to eukaryotic membrane rafts; these rafts include proteins involved in signaling, molecule trafficking and protein secretion. " P39875,"PROTEIN NAMES: Exodeoxyribonuclease 1 (Exodeoxyribonuclease I) (EXO I) (Exonuclease I) (Protein DHS1) PROTEIN FAMILY: XPG/RAD2 endonuclease family, EXO1 subfamily ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the XPG/RAD2 endonuclease family. EXO1 subfamily. FUNCTION: 5'->3' double-stranded DNA exonuclease involved in mismatch repair and eventually also in mitotic recombination between direct repeats. Also has a minor role in the correction of large DNA mismatches that occur in the heteroduplex DNA during meiotic recombination at the HIS4 locus. KEYWORDS: 3D-structure;DNA damage;DNA excision;DNA repair;DNA-binding;Excision nuclease;Exonuclease;Hydrolase;Magnesium;Metal-binding;Nuclease;Nucleus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: Present with 672 molecules/cell in log phase SD medium." P39888,"PROTEIN NAMES: Tetracenomycin A2 monooxygenase-dioxygenase (Tetracenomycin A2 oxygenase) (Tcm A2 oxygenase) (Tetracenomycin polyketide synthesis hydroxylase TcmG) PROTEIN FAMILY: PheA/TfdB FAD monooxygenase family ORGANISM: Streptomyces glaucescens SIMILARITY: Belongs to the PheA/TfdB FAD monooxygenase family. FUNCTION: Involved in the biosynthesis of tetracenomycin C (TCM C). Catalyzes the triple hydroxylation of tetracenomycin A2 (TCM A2) at positions C-4, C-4a and C-12a to give tetracenomycin C (TCM C). Can use either NADH or NADPH as electron donors, but prefers NADPH under physiological conditions. PATHWAY: Antibiotic biosynthesis; tetracenomycin C biosynthesis. KEYWORDS: Antibiotic biosynthesis;Dioxygenase;Direct protein sequencing;FAD;Flavoprotein;Monooxygenase;Oxidoreductase " P39899,PROTEIN NAMES: Neutral protease B PROTEIN FAMILY: Peptidase M4 family ORGANISM: Bacillus subtilis (strain 168) SIMILARITY: Belongs to the peptidase M4 family. FUNCTION: Protease able to cleave casein in vitro. KEYWORDS: Calcium;Direct protein sequencing;Hydrolase;Metal-binding;Metalloprotease;Protease;Reference proteome;Secreted;Signal;Zinc;Zymogen SUBCELLULAR LOCATION: Secreted P39909,"PROTEIN NAMES: Spermine/spermidine N(1)-acetyltransferase PROTEIN FAMILY: Acetyltransferase family ORGANISM: Bacillus subtilis (strain 168) SIMILARITY: Belongs to the acetyltransferase family. FUNCTION: Acetylates both spermidine and spermine at primary propyl amine moieties, with spermine being the preferred substrate. PATHWAY: Amine and polyamine degradation; spermine degradation.; PATHWAY: Amine and polyamine degradation; spermidine degradation. KEYWORDS: Acyltransferase;Reference proteome;Transferase " P39923,"PROTEIN NAMES: Nitrogen permease regulator 2 PROTEIN FAMILY: NPR2 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the NPR2 family. FUNCTION: Component of the SEA complex which coats the vacuolar membrane and is involved in intracellular trafficking, autophagy, response to nitrogen starvation, and amino acid biogenesis. Mediates inactivation of the TORC1 complex in response to amino acid starvation. Post-transcriptional regulator of nitrogen permeases. May be involved in putative NPR1-dependent phosphorylation of nitrogen permeases or in the processing and targeting of nitrogen permeases at the level of the endoplasmic reticulum. KEYWORDS: 3D-structure;Membrane;Phosphoprotein;Protein transport;Reference proteome;Transcription;Transcription regulation;Transport;Vacuole SUBCELLULAR LOCATION: Vacuole membrane ; Peripheral membrane protein MISCELLANEOUS: Present with 319 molecules/cell in log phase SD medium." P39949,"PROTEIN NAMES: G1/S-specific cyclin-E1 PROTEIN FAMILY: Cyclin family, Cyclin E subfamily ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the cyclin family. Cyclin E subfamily. FUNCTION: Essential for the control of the cell cycle at the G1/S (start) transition. KEYWORDS: Cell cycle;Cell division;Cyclin;Nucleus;Phosphoprotein;Reference proteome;Ubl conjugation SUBCELLULAR LOCATION: Nucleus " P39952,PROTEIN NAMES: Mitochondrial inner membrane protein OXA1 (Cytochrome oxidase biogenesis protein OXA1) (Oxidase assembly protein 1) PROTEIN FAMILY: OXA1/ALB3/YidC family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the OXA1/ALB3/YidC family. FUNCTION: Mitochondrial inner membrane insertase that mediates the insertion of both mitochondrion-encoded precursors and nuclear-encoded proteins from the matrix into the inner membrane. Links mitoribosomes with the inner membrane. Forms pores capable of accommodating translocating protein segments. Essential for the activity and assembly of cytochrome c oxidase. Plays a central role in the translocation and export of the N-terminal part of the COX2 protein into the mitochondrial intermembrane space. KEYWORDS: Membrane;Mitochondrion;Mitochondrion inner membrane;Reference proteome;Transit peptide;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Mitochondrion inner membrane ; Multi-pass membrane protein Note=The availability of nuclear- and mitochondrial-encoded substrates influences the inner- membrane distribution. MISCELLANEOUS: Present with 6550 molecules/cell in log phase SD medium. P39998,"PROTEIN NAMES: Enhancer of mRNA-decapping protein 3 PROTEIN FAMILY: EDC3 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the EDC3 family. FUNCTION: Stimulates decapping of both stable and unstable mRNA during mRNA decay. Does not affect nonsense-mediated mRNA decay. Required for normal P-body assembly. KEYWORDS: 3D-structure;Cytoplasm;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cytoplasm, P-body Note=Is concentrated in several cytoplasmic foci called P bodies (or cytoplasmic processing bodies) which represent sites of mRNA decapping and 5' to 3' exonucleotidic decay. MISCELLANEOUS: Present with 2340 molecules/cell in log phase SD medium." P40010,PROTEIN NAMES: Nuclear GTP-binding protein NUG1 (Nuclear GTPase 1) PROTEIN FAMILY: TRAFAC class YlqF/YawG GTPase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the TRAFAC class YlqF/YawG GTPase family. FUNCTION: GTPase required for 60S ribosomal subunit export to the cytoplasm. KEYWORDS: 3D-structure;GTP-binding;Nucleotide-binding;Nucleus;Phosphoprotein;Protein transport;Reference proteome;Ribosome biogenesis;Transport SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: Present with 7130 molecules/cell in log phase SD medium. P40013,"PROTEIN NAMES: Protein BIM1 PROTEIN FAMILY: MAPRE family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the MAPRE family. FUNCTION: Binds microtubules. KEYWORDS: 3D-structure;Acetylation;Cell cycle;Cell division;Cytoplasm;Cytoskeleton;Microtubule;Mitosis;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton. MISCELLANEOUS: Present with 3630 molecules/cell in log phase SD medium." P40016,PROTEIN NAMES: 26S proteasome regulatory subunit RPN3 PROTEIN FAMILY: Proteasome subunit S3 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the proteasome subunit S3 family. FUNCTION: Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins. KEYWORDS: 3D-structure;Acetylation;Direct protein sequencing;Phosphoprotein;Proteasome;Reference proteome MISCELLANEOUS: Present with 16700 molecules/cell in log phase SD medium. P40028,"PROTEIN NAMES: Holliday junction resolvase YEN1 PROTEIN FAMILY: XPG/RAD2 endonuclease family, GEN subfamily ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the XPG/RAD2 endonuclease family. GEN subfamily. FUNCTION: Endonuclease which resolves Holliday junctions by the introduction of symmetrically related cuts across the junction point, to produce nicked duplex products in which the nicks can be readily ligated. Four-way DNA intermediates, also known as Holliday junctions, are formed during homologous recombination and DNA repair, and their resolution is necessary for proper chromosome segregation. Involved in DNA-damage repair in vegetative cells. KEYWORDS: Cytoplasm;DNA damage;DNA repair;Endonuclease;Hydrolase;Nuclease;Nucleus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Nucleus Note=Predominantly nuclear in G1-arrested cells, but cytoplasmically localized after release from G1 arrest. MISCELLANEOUS: Present with 131 molecules/cell in log phase SD medium." P40051,"PROTEIN NAMES: Intermediate cleaving peptidase 55 (Intermediate cleaving peptidase of 55 kDa) PROTEIN FAMILY: Peptidase M24B family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the peptidase M24B family. FUNCTION: Aminopeptidase which cleaves preprotein intermediates that carry destabilizing N-ter amino acid residues after the mitochondrial processing peptidase (MPP) cleavage site and is thus critical for stabilization of the mitochondrial proteome. KEYWORDS: 3D-structure;Aminopeptidase;Hydrolase;Manganese;Membrane;Metal-binding;Metalloprotease;Mitochondrion;Mitochondrion inner membrane;Nucleus;Protease;Reference proteome SUBCELLULAR LOCATION: Nucleus Mitochondrion inner membrane ; Peripheral membrane protein ; Matrix side Note=Has the same dual localization (mitochondrion and nucleus) as one of its substrate, NFS1. MISCELLANEOUS: Present with 5080 molecules/cell in log phase SD medium." P40066,"PROTEIN NAMES: mRNA export factor GLE2 (Nuclear pore protein GLE2) (Nucleoporin GLE2) (poly(A) RNA export protein RAE1) PROTEIN FAMILY: WD repeat rae1 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the WD repeat rae1 family. FUNCTION: Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. It is specifically important for nuclear mRNA export. KEYWORDS: Membrane;mRNA transport;Nuclear pore complex;Nucleus;Protein transport;Reference proteome;Repeat;Translocation;Transport;WD repeat SUBCELLULAR LOCATION: Nucleus, nuclear pore complex Nucleus membrane; Peripheral membrane protein; Cytoplasmic side. Nucleus membrane; Peripheral membrane protein; Nucleoplasmic side. Note=Symmetric distribution. MISCELLANEOUS: Present with 1820 molecules/cell in log phase SD medium." P40084,PROTEIN NAMES: RNA polymerase II subunit B1 CTD phosphatase RTR1 (RNA polymerase II-associated protein 2 homolog RTR1) (Regulator of transcription 1) PROTEIN FAMILY: RPAP2 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the RPAP2 family. FUNCTION: RNA polymerase II subunit B1 C-terminal domain (CTD) phosphatase that dephosphorylates 'Ser-5' of the CTD and regulates RNA polymerase II during the transition from 'Ser-5' to 'Ser-2' phosphorylation. KEYWORDS: 3D-structure;Cytoplasm;Hydrolase;Metal-binding;Nucleus;Protein phosphatase;Reference proteome;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Shuttles constitutively between the cytoplasm and the nucleus. MISCELLANEOUS: Present with 5480 molecules/cell in log phase SD medium. P40087,PROTEIN NAMES: DNA damage-inducible protein 1 (v-SNARE-master 1) PROTEIN FAMILY: DDI1 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the DDI1 family. FUNCTION: Aspartic protease. Appears to act as negative regulator of constitutive exocytosis. May act at the level of secretory vesicle docking and fusion as a competitive inhibitor of SNARE assembly. Acts as a linker between the 19S proteasome and polyubiquitinated proteins like the HO endonuclease and UFO1 via UBA domain interactions with ubiquitin for their subsequent degradation. Required for S-phase checkpoint control. KEYWORDS: 3D-structure;Aspartyl protease;Cell membrane;Cytoplasm;Hydrolase;Isopeptide bond;Membrane;Protease;Protein transport;Reference proteome;Transport;Ubl conjugation SUBCELLULAR LOCATION: Cytoplasm Cell membrane ; Peripheral membrane protein ; Cytoplasmic side MISCELLANEOUS: Present with 6510 molecules/cell in log phase SD medium. P40106,"PROTEIN NAMES: Glycerol-1-phosphate phosphohydrolase 2 ((DL)-glycerol-3-phosphatase 2) (Hyperosmolarity-responsive protein 2) PROTEIN FAMILY: HAD-like hydrolase superfamily, DOG/GPP family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the HAD-like hydrolase superfamily. DOG/GPP family. FUNCTION: Glycerol-1-phosphate phosphohydrolase involved in glycerol biosynthesis. Plays a role in osmoadaptation. KEYWORDS: Cytoplasm;Direct protein sequencing;Hydrolase;Isopeptide bond;Magnesium;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Stress response;Ubl conjugation SUBCELLULAR LOCATION: Cytoplasm Nucleus MISCELLANEOUS: Present with 5000 molecules/cell in log phase SD medium." P40160,"PROTEIN NAMES: Serine/threonine-protein kinase RIO2 PROTEIN FAMILY: Protein kinase superfamily, RIO-type Ser/Thr kinase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the protein kinase superfamily. RIO-type Ser/Thr kinase family. FUNCTION: Required for the final endonucleolytic cleavage of 20S pre-rRNA at site D in the cytoplasm, converting it into the mature 18S rRNA. Involved in normal export of the pre-40S particles from the nucleus to the cytoplasm. No longer associates with pre-40S subunits in RPS19 disruptions, suggesting it acts after the ribosomal protein in 18S rRNA maturation. KEYWORDS: 3D-structure;ATP-binding;Cytoplasm;Kinase;Magnesium;Metal-binding;Nucleotide-binding;Nucleus;Phosphoprotein;Reference proteome;Ribosome biogenesis;Serine/threonine-protein kinase;Transferase SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Predominantly cytoplasmic. MISCELLANEOUS: Present with 10300 molecules/cell in log phase SD medium." P40186,"PROTEIN NAMES: PHO85 cyclin-7 (PHO85-associated protein 1) PROTEIN FAMILY: Cyclin family, PHO80 subfamily ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the cyclin family. PHO80 subfamily. FUNCTION: Cyclin partner of the cyclin-dependent kinase (CDK) PHO85. Together with cyclin PCL6, controls glycogen phosphorylase and glycogen synthase activities in response to nutrient availablility. The PCL7-PHO85 cyclin-CDK holoenzyme has GLC8 kinase activity and phosphorylates and inactivates the phosphatase PP1-2 inhibitor GLC8, causing activation of PP1-2, which then dephosphorylates and activates glycogen phosphorylase. PCL7-PHO85 also phosphorylates MMR1 and YJL084C. KEYWORDS: Carbohydrate metabolism;Cell cycle;Cell division;Cyclin;Cytoplasm;Direct protein sequencing;Glycogen metabolism;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cytoplasm MISCELLANEOUS: Present with 3390 molecules/cell in log phase SD medium." P40202,PROTEIN NAMES: Superoxide dismutase 1 copper chaperone PROTEIN FAMILY: CCS1 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the CCS1 family. FUNCTION: Copper chaperone for apo superoxide dismutase 1 (SOD1). Binds copper ions and delivers them specifically to apo-SOD1. KEYWORDS: 3D-structure;Chaperone;Copper;Cytoplasm;Disulfide bond;Metal-binding;Mitochondrion;Reference proteome;Zinc SUBCELLULAR LOCATION: Cytoplasm. Mitochondrion intermembrane space Note=A small percentage (around 1-5 percent) localizes to the mitochondrial intermembrane space. MISCELLANEOUS: Present with 12000 molecules/cell in log phase SD medium. P40228,"PROTEIN NAMES: Casein kinase II subunit beta-1 (CK II beta-1) PROTEIN FAMILY: Casein kinase 2 subunit beta family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the casein kinase 2 subunit beta family. FUNCTION: Plays a complex role in regulating the basal catalytic activity of the alpha subunit. The tetrameric holoenzyme CK2, composed of two alpha and two beta subunits, phosphorylates the transcription factor GBFl, resulting in stimulation of its DNA binding activity. CK2 phosphorylates the transcription factor PIF1 after an exposure to light, resulting in a proteasome-dependent degradation of PIF1 and promotion of photomorphogenesis. CK2 phosphorylates translation initiation factors. May participate in the regulation of the initiation of translation. Stimulates the binding of CCA1 to promoters (Probable). KEYWORDS: Alternative splicing;Cytoplasm;Nucleus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cytoplasm, cytosol Nucleus " P40259,"PROTEIN NAMES: B-cell antigen receptor complex-associated protein beta chain (B-cell-specific glycoprotein B29) (Ig-beta) (Immunoglobulin-associated B29 protein) (CD antigen CD79b) ORGANISM: Homo sapiens (Human) FUNCTION: Required in cooperation with CD79A for initiation of the signal transduction cascade activated by the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. Enhances phosphorylation of CD79A, possibly by recruiting kinases which phosphorylate CD79A or by recruiting proteins which bind to CD79A and protect it from dephosphorylation. KEYWORDS: 3D-structure;Adaptive immunity;Alternative splicing;Cell membrane;Direct protein sequencing;Disease variant;Disulfide bond;Glycoprotein;Immunity;Immunoglobulin domain;Membrane;Phosphoprotein;Reference proteome;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. Note=Following antigen binding, the BCR has been shown to translocate from detergent-soluble regions of the cell membrane to lipid rafts although signal transduction through the complex can also occur outside lipid rafts. " P40308,"PROTEIN NAMES: Triacylglycerol lipase 3 (Lipase 3) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Lipid particle-localized triacylglycerol (TAG) lipase. The lipid droplet/particle is a lipid storage compartment which serves as a depot of energy and building blocks for membrane lipid biosynthesis. Involved in the mobilization of the non-polar storage lipids triacylglycerols (TAGs) from lipid particles by hydrolysis of TAGs, releasing and supplying specific fatty acids to the appropriate metabolic pathways. Also catalyzes the acylation of lysophosphatidic acid (LPA). Important for efficient sporulation, but rather through its acyltransferase than lipase activity. KEYWORDS: Hydrolase;Lipid degradation;Lipid droplet;Lipid metabolism;Reference proteome SUBCELLULAR LOCATION: Lipid droplet Note=Partially retained in the endoplasmic reticulum in cells lacking triacylglycerols. MISCELLANEOUS: Present with 3210 molecules/cell in log phase SD medium." P40335,PROTEIN NAMES: Carboxypeptidase Y-deficient protein 8 (Vacuolar protein sorting-associated protein 26) (Vacuolar protein-targeting protein 4) PROTEIN FAMILY: VPS26 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the VPS26 family. FUNCTION: Plays a role in vesicular protein sorting. Required for the endosome-to-Golgi retrieval of the vacuolar protein sorting receptor VPS10. Component of the membrane-associated retromer complex which is essential in endosome-to-Golgi retrograde transport. The VPS29-VPS26-VPS35 subcomplex may be involved in cargo selection. KEYWORDS: Phosphoprotein;Protein transport;Reference proteome;Transport P40356,"PROTEIN NAMES: Mediator of RNA polymerase II transcription subunit 3 (Hyper-recombination suppressor protein 1) (Mediator complex subunit 3) (Poly-glutamine domain protein 1) PROTEIN FAMILY: Mediator complex subunit 3 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the mediator complex subunit 3 family. FUNCTION: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. The Mediator complex unfolds to an extended conformation and partially surrounds RNA polymerase II, specifically interacting with the unphosphorylated form of the C-terminal domain (CTD) of RNA polymerase II. The Mediator complex dissociates from the RNA polymerase II holoenzyme and stays at the promoter when transcriptional elongation begins. PGD1/MED3 is also involved in direct repeat recombination. KEYWORDS: 3D-structure;Acetylation;Activator;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: Present with 556 molecules/cell in log phase SD medium." P40368,"PROTEIN NAMES: Nucleoporin NUP82 (Nuclear pore protein NUP82) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. It is specifically involved as part of the NUP82-NUP159-NSP1 subcomplex in nuclear mRNA and pre-ribosome export by acting as a linker tethering nucleoporins that are directly involved in nuclear transport to the NPC via its coiled-coil domain. KEYWORDS: 3D-structure;Coiled coil;Membrane;mRNA transport;Nuclear pore complex;Nucleus;Protein transport;Reference proteome;Translocation;Transport SUBCELLULAR LOCATION: Nucleus, nuclear pore complex Nucleus membrane; Peripheral membrane protein; Cytoplasmic side. MISCELLANEOUS: Present with 9470 molecules/cell in log phase SD medium." P40371,PROTEIN NAMES: Protein phosphatase 2C homolog 1 (PP2C-1) PROTEIN FAMILY: PP2C family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the PP2C family. FUNCTION: It has a serine and threonine phosphatase activity. Has a specialized role in the heat shock response. May be responsible for the dephosphorylation of hsp90. Also has a role in maintaining osmotic stability. KEYWORDS: Hydrolase;Magnesium;Manganese;Metal-binding;Protein phosphatase;Reference proteome;Stress response P40384,PROTEIN NAMES: Meiotic recombination protein rec12 (SPO11 protein homolog) PROTEIN FAMILY: TOP6A family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the TOP6A family. FUNCTION: Required for formation of the double-strand breaks (DSBs) that initiate meiotic recombination. Required for crossover recombination and chiasmatic segregation of chromosomes during meiosis I. Also involved in the faithful equational segregation of chromosomes during meiosis II. KEYWORDS: Chromosome partition;Cytoplasm;DNA-binding;Isomerase;Magnesium;Meiosis;Metal-binding;Nucleus;Reference proteome;Topoisomerase SUBCELLULAR LOCATION: Cytoplasm Nucleus P40386,PROTEIN NAMES: Probable thiamine biosynthetic bifunctional enzyme [Includes: Thiamine-phosphate synthase (TP synthase) (TPS) (Thiamine-phosphate pyrophosphorylase) (TMP pyrophosphorylase) (TMP-PPase); Hydroxyethylthiazole kinase (4-methyl-5-beta-hydroxyethylthiazole kinase) (TH kinase) (THZ kinase)] PROTEIN FAMILY: Thiamine-phosphate synthase family; Thz kinase family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: In the N-terminal section; belongs to the thiamine-phosphate synthase family.; SIMILARITY: In the C-terminal section; belongs to the Thz kinase family. FUNCTION: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP). PATHWAY: Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole: step 1/1.; PATHWAY: Cofactor biosynthesis; thiamine diphosphate biosynthesis; thiamine phosphate from 4-amino-2-methyl-5-diphosphomethylpyrimidine and 4-methyl-5-(2-phosphoethyl)-thiazole: step 1/1. KEYWORDS: ATP-binding;Kinase;Magnesium;Metal-binding;Multifunctional enzyme;Nucleotide-binding;Reference proteome;Thiamine biosynthesis;Transferase P40421,PROTEIN NAMES: Serine/threonine-protein phosphatase rdgC (Retinal degeneration C protein) PROTEIN FAMILY: PPP phosphatase family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the PPP phosphatase family. FUNCTION: Phosphatase required to prevent light-induced retinal degeneration. KEYWORDS: Calcium;Hydrolase;Magnesium;Manganese;Metal-binding;Protein phosphatase;Reference proteome;Repeat;Sensory transduction;Vision P40465,PROTEIN NAMES: Chromosome segregation in meiosis protein 2 PROTEIN FAMILY: CSM2 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the CSM2 family. FUNCTION: Involved in chromosome segregation during meiosis. Promotes efficient recombinational repair and functions in the protection of the genome from spontaneous and induced DNA damage like mutations and gross chromosomal rearrangements (GCRs). KEYWORDS: 3D-structure;Cytoplasm;DNA damage;DNA repair;Meiosis;Nucleus;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Nucleus MISCELLANEOUS: Present with 414 molecules/cell in log phase SD medium. P40466,"PROTEIN NAMES: Fork head protein homolog 1 ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Transcription factor that regulates the expression of the CLB2 cluster of genes during the G2/M phase of the mitotic cell cycle. The CLB2 cluster of genes includes mitotic regulators such as CLB1, CLB2, CDC5 and CDC20 as well as SWI5 and ACE2, transcription factors required for the subsequent temporal wave of cell cycle regulated gene expression in the M/G1 phase interval. Involved in HMRa silencing. FKH1 and FKH2 associate with the coding regions of active genes and influence, in opposing ways, transcriptional elongation and termination, and coordinate early transcription elongation and pre-mRNA processing. Both FKH1 and FKH2 play a role as regulators of lifespan in collaboration with the anaphase-promoting complex (APC), likely through combined regulation of stress response, genomic stability, and cell cycle regulation. FKH1 and FKH2 function also in controlling yeast cell morphology by preventing preudohyphal growth. Acts as a rate-limiting replication origin activator via its interaction with the origin recognition complex (ORC). Plays a transcription-independent role in recombination donor preference during mating-type switching through binding to the recombination enhancer (RE), a 700-bp cis-acting element that controls recombination along the left arm of chromosome III. KEYWORDS: Cytoplasm;DNA-binding;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus Cytoplasm, cytosol Note=Relocalizes to the cytosol in response to hypoxia. MISCELLANEOUS: Present with 1720 molecules/cell in log phase SD medium." P40484,"PROTEIN NAMES: DBF2 kinase activator protein MOB1 (MPS1 binder 1) (Maintenance of ploidy protein MOB1) PROTEIN FAMILY: MOB1/phocein family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the MOB1/phocein family. FUNCTION: Functions as an activator subunit for the DBF2 protein kinase. Binds to DBF2, which is required for the phosphorylation and activation of DBF2 by the upstream kinase CDC15 in late anaphase. DBF2-MOB1 is part of the mitotic exit network (MEN) signaling cascade, which regulates release from the nucleus and activity of phosphatase CDC14. Required for inactivation of mitotic cyclin-dependent kinase for exit from mitosis, cytokinesis and G1 gene transcription. KEYWORDS: 3D-structure;Cell cycle;Cell division;Centromere;Chromosome;Cytoplasm;Cytoskeleton;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Zinc SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body. Bud neck. Chromosome, centromere. Note=Localizes predominantly to the cytoplasmic surface of the spindle pole body during anaphase and to a ring at the bud neck during cytokinesis. Translocates to the nucleus during mitosis and associates with centromere DNA. MISCELLANEOUS: Present with 5020 molecules/cell in log phase SD medium." P40485,"PROTEIN NAMES: Phosphatidylinositol 4,5-bisphosphate-binding protein SLM1 (Synthetic lethal with MSS4 protein 1) (TORC2 effector protein SLM1) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Together with SLM2, effector of the TORC2- and calcineurin-signaling pathways. Phosphorylated and activated by TORC2 under favorable growth conditions. Mediates actin polarization via inhibition of calcineurin-dependent transcription. Upon nutrient limitation or environmental stress, gets dephosphorylated by calcineurin. Dephosphorylation inhibits its interaction with TORC2, thereby antagonizing TORC2 signaling and mediating calcineurin-dependent actin depolarization. Also functions in heat-induced, calcineurin-mediated uracil permease (FUR4) endocytosis. KEYWORDS: 3D-structure;Cell membrane;Coiled coil;Membrane;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cell membrane ; Peripheral membrane protein ; Cytoplasmic side Note=Localizes to cortical punctate structures. Correct localization requires phosphatidylinositol 4,5-bisphosphate and functional TORC2. MISCELLANEOUS: Present with 5190 molecules/cell in log phase SD medium." P40494,"PROTEIN NAMES: Actin-regulating kinase PRK1 (p53-regulating kinase 1) PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. FUNCTION: Protein kinase involved in the regulation of actin cytoskeleton organization and endocytosis. Phosphorylates PAN1 which disrupts the interaction between PAN1 and END3, and between PAN1 and SLA1. Phosphorylates SCD5. Preferentially, phosphorylates substrates on threonine residues in a [L/I/V/M]-x-x-[Q/N/T/S]-x-T-G motif. KEYWORDS: ATP-binding;Cytoplasm;Cytoskeleton;Kinase;Nucleotide-binding;Phosphoprotein;Reference proteome;Serine/threonine-protein kinase;Transferase SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, actin patch Note=Cortical actin patches. MISCELLANEOUS: Present with 1323 molecules/cell in log phase SD medium." P40501,PROTEIN NAMES: Vacuolar amino acid transporter 7 PROTEIN FAMILY: Amino acid/polyamine transporter 2 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the amino acid/polyamine transporter 2 family. FUNCTION: Probable amino acid transporter of unknown specificity. KEYWORDS: Amino-acid transport;Membrane;Reference proteome;Transmembrane;Transmembrane helix;Transport;Vacuole SUBCELLULAR LOCATION: Vacuole membrane ; Multi-pass membrane protein MISCELLANEOUS: Present with 195 molecules/cell in log phase SD medium. P40506,"PROTEIN NAMES: Phosphopantothenate--cysteine ligase CAB2 (Coenzyme A biosynthesis protein 2) (Phosphopantothenoylcysteine synthetase) (PPC synthetase) PROTEIN FAMILY: PPC synthetase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the PPC synthetase family. FUNCTION: Catalyzes the first step in the biosynthesis of coenzyme A from vitamin B5, where cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine. PATHWAY: Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 2/5. KEYWORDS: 3D-structure;Coenzyme A biosynthesis;Cytoplasm;Ligase;Nucleus;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Nucleus MISCELLANEOUS: Present with 3170 molecules/cell in log phase SD medium." P40509,"PROTEIN NAMES: Coatomer subunit epsilon (Epsilon-coat protein) (Epsilon-COP) PROTEIN FAMILY: COPE family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the COPE family. FUNCTION: The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. The coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. KEYWORDS: 3D-structure;Cytoplasm;Cytoplasmic vesicle;ER-Golgi transport;Golgi apparatus;Membrane;Protein transport;Reference proteome;Transport SUBCELLULAR LOCATION: Cytoplasm Golgi apparatus membrane ; Peripheral membrane protein ; Cytoplasmic side Cytoplasmic vesicle, COPI-coated vesicle membrane ; Peripheral membrane protein ; Cytoplasmic side Note=The coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, as well as on the vesicles/buds originating from it. MISCELLANEOUS: Present with 23100 molecules/cell in log phase SD medium." P40559,"PROTEIN NAMES: Phosphatidylinositol 4,5-bisphosphate 5-phosphatase INP51 (Synaptojanin-like protein 1) PROTEIN FAMILY: Synaptojanin family; Inositol 1,4,5-trisphosphate 5-phosphatase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the synaptojanin family.; SIMILARITY: In the central section; belongs to the inositol 1,4,5-trisphosphate 5-phosphatase family. FUNCTION: Controls the cellular levels and subcellular distribution of phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). Does not utilize phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2), nor phosphatidylinositol 3-phosphate (PtdIns(3)P) and phosphatidylinositol 4-phosphate (PtdIns(4)P). Plays an essential role in a TGN (trans Golgi network)-to-early endosome pathway. Involved in endocytosis and acts as a negative regulator of the Slm pathway which modulates polarized actin assembly and growth. KEYWORDS: Cytoplasm;Cytoskeleton;Endocytosis;Hydrolase;Lipid metabolism;Protein transport;Reference proteome;Transport SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, actin patch MISCELLANEOUS: Present with 98 molecules/cell in log phase SD medium." P40577,"PROTEIN NAMES: Anaphase-promoting complex subunit MND2 (Meiotic nuclear division protein 2) PROTEIN FAMILY: APC15 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the APC15 family. FUNCTION: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin-protein ligase complex that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C is thought to confer substrate specificity and, in the presence of ubiquitin-conjugating E2 enzymes, it catalyzes the formation of protein-ubiquitin conjugates that are subsequently degraded by the 26S proteasome. In early mitosis, the APC/C is activated by CDC20 and targets securin PDS1, the B-type cyclin CLB5, and other anaphase inhibitory proteins for proteolysis, thereby triggering the separation of sister chromatids at the metaphase-to-anaphase transition. In late mitosis and in G1, degradation of CLB5 allows activation of the APC/C by CDH1, which is needed to destroy CDC20 and the B-type cyclin CLB2 to allow exit from mitosis and creating the low CDK state necessary for cytokinesis and for reforming prereplicative complexes in G1 prior to another round of replication. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: 3D-structure;Cell cycle;Cell division;Mitosis;Phosphoprotein;Reference proteome;Ubl conjugation pathway " P40689,"PROTEIN NAMES: Torso-like protein ORGANISM: Drosophila melanogaster (Fruit fly) FUNCTION: Probable ligand that binds to the torso receptor. Implicated in a receptor tyrosine kinase signaling pathway that specifies terminal cell fate. KEYWORDS: Developmental protein;Glycoprotein;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted Note=Is a component of the vitelline membrane (VM, the inner layer of the eggshell). " P40764,"PROTEIN NAMES: Homeobox protein DLX-2 (Homeobox protein TES-1) PROTEIN FAMILY: Distal-less homeobox family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the distal-less homeobox family. FUNCTION: Acts as a transcriptional activator. Activates transcription of CGA/alpha-GSU, via binding to the downstream activin regulatory element (DARE) in the gene promoter. Plays a role in terminal differentiation of interneurons, such as amacrine and bipolar cells in the developing retina. Likely to play a regulatory role in the development of the ventral forebrain. May play a role in craniofacial patterning and morphogenesis. KEYWORDS: Activator;Developmental protein;Differentiation;DNA-binding;Homeobox;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " P40767,"PROTEIN NAMES: Peptidoglycan DL-endopeptidase CwlO (D-gamma-glutamyl-meso-diaminopimelyl DL-endopeptidase) (PSPA2) PROTEIN FAMILY: Peptidase C40 family ORGANISM: Bacillus subtilis (strain 168) SIMILARITY: Belongs to the peptidase C40 family. FUNCTION: The C-terminal part of CwlO shows a cell wall hydrolytic DL-endopeptidase activity. KEYWORDS: Cell wall;Cell wall biogenesis/degradation;Hydrolase;Protease;Reference proteome;Secreted;Signal;Thiol protease SUBCELLULAR LOCATION: Secreted Secreted, cell wall MISCELLANEOUS: The N-terminal part of CwlO was shown to have no cell wall-binding activity." P40791,"PROTEIN NAMES: Myocyte-specific enhancer factor 2 (D-mef2) (MADS domain transcription factor) PROTEIN FAMILY: MEF2 family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the MEF2 family. FUNCTION: Transcription factor that could be a key player in early mesoderm differentiation and may be required for subsequent cell fate specifications within the somatic and visceral/heart mesodermal layers. Essential for myoblast fusion and consequently muscle formation in adults. During embryonic and pupal development, binds to the enhancer of the myoblast fusion gene sing and activates its transcription. KEYWORDS: Activator;Alternative splicing;Developmental protein;Differentiation;DNA-binding;Myogenesis;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " P40795,"PROTEIN NAMES: Protein folded gastrulation [Cleaved into: Protein folded gastrulation; G protein-coupled receptor ligand] ORGANISM: Drosophila melanogaster (Fruit fly) FUNCTION: Coordinates cell shape changes during formation of the ventral furrow and invagination of the posterior midgut primordium, by inducing apical constriction of cells in spatially and temporally defined manners. Could function as a secreted signal to initiate apical constriction by acting as a ligand for an unidentified G protein-coupled receptor, which in turn activates the G protein alpha subunit encoded by concertina, in neighboring cells. Such an intracellular pathway would ultimately induce contraction of the apical actin-myosin network. In the ventral furrow, fog appears to ensure that all the cells initiate constriction within several minutes of each other. In the posterior midgut invagination, fog appears to direct the ordered progression of constriction initiations out from a central region and also to delimit the peripheral extent of this spreading. KEYWORDS: Cell shape;Developmental protein;Extracellular matrix;Gastrulation;Glycoprotein;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix " P40809,PROTEIN NAMES: GTPase-activating protein RacGAP84C (Protein rotund RacGAP) ORGANISM: Drosophila melanogaster (Fruit fly) FUNCTION: Involved in the morphogenesis of the adult appendages. GTPase-activating protein for p21-Rac. Promotes the exchange of Rac-bound GDP by GTP. KEYWORDS: Alternative splicing;Developmental protein;Differentiation;Guanine-nucleotide releasing factor;Metal-binding;Reference proteome;Spermatogenesis;Zinc;Zinc-finger P40817,"PROTEIN NAMES: Diaminopropionate ammonia-lyase (DAPAL) (2,3-diaminopropionate ammonia-lyase) (Alpha,beta-diaminopropionate ammonia-lyase) (Diaminopropionatase) PROTEIN FAMILY: Diaminopropionate ammonia-lyase family ORGANISM: Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) SIMILARITY: Belongs to the diaminopropionate ammonia-lyase family. FUNCTION: Catalyzes the alpha,beta-elimination reaction of both L- and D-alpha,beta-diaminopropionate (DAP) to form pyruvate and ammonia. In vitro L- and D-isomers of serine are also degraded, though slowly; it is the only serine dehydratase which can eliminate an amino group at the beta-carbon position. In vivo L-, D- and a mixure of DL-DAP allow growth. DL-DAP is toxic in the absence of this enzyme, it may inhibit enzymes involved in the synthesis of pyruvate and aspartate, as well as amino acids derived from them. KEYWORDS: 3D-structure;Direct protein sequencing;Lyase;Pyridoxal phosphate;Reference proteome " P40850,"PROTEIN NAMES: Post-transcriptional regulator MKT1 (Inactive endonuclease MKT1) PROTEIN FAMILY: XPG/RAD2 endonuclease family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the XPG/RAD2 endonuclease family. FUNCTION: Involved in 3'-UTR mediated RNA regulation. Binds to RNA-binding and RNA regulatory proteins. Complexes with PAB1-binding protein to promote mRNA interactions with poly(A)-binding protein. Promotes mating-type switching in mother cells by positively regulating HO expression. KEYWORDS: Cytoplasm;Isopeptide bond;Phosphoprotein;Reference proteome;Translation regulation;Ubl conjugation SUBCELLULAR LOCATION: Cytoplasm, cytosol Note=Localizes to polysomes in a PBP1-dependent manner. MISCELLANEOUS: Present with 3430 molecules/cell in log phase SD medium." P40855,"PROTEIN NAMES: Peroxisomal biogenesis factor 19 (33 kDa housekeeping protein) (Peroxin-19) (Peroxisomal farnesylated protein) PROTEIN FAMILY: Peroxin-19 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the peroxin-19 family. FUNCTION: Necessary for early peroxisomal biogenesis. Acts both as a cytosolic chaperone and as an import receptor for peroxisomal membrane proteins (PMPs). Binds and stabilizes newly synthesized PMPs in the cytoplasm by interacting with their hydrophobic membrane-spanning domains, and targets them to the peroxisome membrane by binding to the integral membrane protein PEX3. Excludes CDKN2A from the nucleus and prevents its interaction with MDM2, which results in active degradation of TP53. KEYWORDS: 3D-structure;Acetylation;Alternative splicing;Cytoplasm;Host-virus interaction;Lipoprotein;Membrane;Methylation;Peroxisome;Peroxisome biogenesis;Peroxisome biogenesis disorder;Phosphoprotein;Prenylation;Reference proteome;Zellweger syndrome SUBCELLULAR LOCATION: Cytoplasm Peroxisome membrane ; Lipid-anchor ; Cytoplasmic side Note=Mainly cytoplasmic. Some fraction membrane-associated to the outer surface of peroxisomes. MISCELLANEOUS: [Isoform 1]: The two main transcripts are PXF-all and PXF-delta-2.; MISCELLANEOUS: [Isoform 2]: The two main transcripts are PXF-all and PXF-delta-2.; MISCELLANEOUS: [Isoform 6]: May be produced at very low levels due to a premature stop CC codon in the mRNA, leading to nonsense-mediated mRNA decay." P40857,"PROTEIN NAMES: Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase PHS1 (3-hydroxyacyl-CoA dehydratase PHS1) (HACD) (PTPLA homolog involved in sphingolipid biosynthesis protein 1) PROTEIN FAMILY: Very long-chain fatty acids dehydratase HACD family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the very long-chain fatty acids dehydratase HACD family. FUNCTION: Catalyzes the third of the four reactions of the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process, allows the addition of two carbons to the chain of long- and very long-chain fatty acids/VLCFAs per cycle. This enzyme catalyzes the dehydration of the 3-hydroxyacyl-CoA intermediate into trans-2,3-enoyl-CoA, within each cycle of fatty acid elongation. Thereby, it participates in the production of VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators. PATHWAY: Lipid metabolism; fatty acid biosynthesis. KEYWORDS: Endoplasmic reticulum;Fatty acid biosynthesis;Fatty acid metabolism;Lipid biosynthesis;Lipid metabolism;Lyase;Membrane;Reference proteome;Transmembrane;Transmembrane helix;Vacuole SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein Vacuole membrane ; Multi-pass membrane protein " P40859,"PROTEIN NAMES: Monomeric sarcosine oxidase (MSOX) PROTEIN FAMILY: MSOX/MTOX family, MSOX subfamily ORGANISM: Bacillus sp. (strain B-0618) SIMILARITY: Belongs to the MSOX/MTOX family. MSOX subfamily. FUNCTION: Catalyzes the oxidative demethylation of sarcosine. Can also oxidize other secondary amino acids such as N-methyl-L-alanine. KEYWORDS: 3D-structure;Cytoplasm;Direct protein sequencing;FAD;Flavoprotein;Oxidoreductase SUBCELLULAR LOCATION: Cytoplasm. " P40879,PROTEIN NAMES: Chloride anion exchanger (Down-regulated in adenoma) (Protein DRA) (Solute carrier family 26 member 3) PROTEIN FAMILY: SLC26A/SulP transporter family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the SLC26A/SulP transporter (TC 2.A.53) family. FUNCTION: Mediates chloride-bicarbonate exchange with a chloride bicarbonate stoichiometry of 2:1 in the intestinal epithelia. Plays a role in the chloride and bicarbonate homeostasis during sperm epididymal maturation and capacitation (By similarity). KEYWORDS: 3D-structure;Antiport;Cell membrane;Chloride;Disease variant;Glycoprotein;Membrane;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Apical cell membrane ; Multi-pass membrane protein Membrane ; Multi-pass membrane protein Cell membrane ; Multi-pass membrane protein Note=Localized in sperm membranes. Midpiece of sperm tail. Colocalizes with CFTR at the midpiece of sperm tail (By similarity). P40953,"PROTEIN NAMES: Chitinase 2 PROTEIN FAMILY: Glycosyl hydrolase 18 family, Chitinase class III subfamily ORGANISM: Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) SIMILARITY: Belongs to the glycosyl hydrolase 18 family. Chitinase class III subfamily. FUNCTION: Chitinase involved in the remodeling of chitin in the fungal cell wall. Plays a role in cell separation. KEYWORDS: Carbohydrate metabolism;Cell wall;Chitin degradation;Chitin-binding;Glycoprotein;Glycosidase;GPI-anchor;Hydrolase;Lipoprotein;Membrane;Polysaccharide degradation;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted, cell wall. Membrane; Lipid-anchor, GPI-anchor. " P40954,"PROTEIN NAMES: Chitinase 3 PROTEIN FAMILY: Glycosyl hydrolase 18 family, Chitinase class III subfamily ORGANISM: Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) SIMILARITY: Belongs to the glycosyl hydrolase 18 family. Chitinase class III subfamily. FUNCTION: Chitinase involved in the remodeling of chitin in the fungal cell wall. Plays a role in cell separation. KEYWORDS: Carbohydrate metabolism;Chitin degradation;Chitin-binding;Glycoprotein;Glycosidase;Hydrolase;Polysaccharide degradation;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted " P40967,"PROTEIN NAMES: Melanocyte protein PMEL (ME20-M) (ME20M) (Melanocyte protein Pmel 17) (Melanocytes lineage-specific antigen GP100) (Melanoma-associated ME20 antigen) (P1) (P100) (Premelanosome protein) (Silver locus protein homolog) [Cleaved into: M-alpha (95 kDa melanocyte-specific secreted glycoprotein) (P26) (Secreted melanoma-associated ME20 antigen) (ME20-S) (ME20S); M-beta] PROTEIN FAMILY: PMEL/NMB family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the PMEL/NMB family. FUNCTION: Forms physiological amyloids that play a central role in melanosome morphogenesis and pigmentation. The maturation of unpigmented premelanosomes from stage I to II is marked by assembly of processed amyloidogenic fragments into parallel fibrillar sheets, which elongate the vesicle into a striated ellipsoidal shape. In pigmented stage III and IV melanosomes, the amyloid matrix serves as a platform where eumelanin precursors accumulate at high local concentrations for pigment formation. May prevent pigmentation-associated toxicity by sequestering toxic reaction intermediates of eumelanin biosynthesis pathway.; FUNCTION: Represents a potent melanoma-specific antigen. Among melanoma non-mutated self-peptides, G9-154 (KTWGQYWQV), G9-209 (ITDQVPFSV) and G9-280 (YLEPGPVTA), appear to act as immunodominant common epitopes that stimulate anti-tumor immune response mediated by HLA-A-restricted cytotoxic T cells. KEYWORDS: 3D-structure;Alternative splicing;Cleavage on pair of basic residues;Direct protein sequencing;Disulfide bond;Endoplasmic reticulum;Endosome;Glycoprotein;Golgi apparatus;Melanin biosynthesis;Membrane;Reference proteome;Repeat;Secreted;Sialic acid;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Single-pass type I membrane protein Golgi apparatus, cis-Golgi network membrane ; Single-pass type I membrane protein Endosome, multivesicular body Melanosome Extracellular vesicle Secreted Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Localizes predominantly to intralumenal vesicles (ILVs) within multivesicular bodies. Associates with ILVs found within the lumen of premelanosomes and melanosomes and particularly in compartments that serve as precursors to the striated stage II premelanosomes. Sorted to stage I melanosomes following its processing in the ER and cis-Golgi. Transiently expressed at the cell surface before targeting to early melanosomes. Colocalizes with BACE2 in stage I and II melanosomes. Colocalizes with CD63 and APOE at exosomes and in intraluminal vesicles within multivesicular endosomes. " P40969,"PROTEIN NAMES: Centromere DNA-binding protein complex CBF3 subunit B (Centromere protein 3) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Acts as a component of the centromere DNA-binding protein complex CBF3, which is essential for chromosome segregation and movement of centromeres along microtubules. CBF3 is required for the recruitment of other kinetochore complexes to CEN DNA. It plays a role in the attachment of chromosomes to the spindle and binds selectively to a highly conserved DNA sequence called CDEIII, found in centromers and in several promoters. KEYWORDS: 3D-structure;Centromere;Chromosome;DNA-binding;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Zinc SUBCELLULAR LOCATION: Nucleus Chromosome, centromere. MISCELLANEOUS: Present with 1900 molecules/cell in log phase SD medium." P40988,PROTEIN NAMES: Low-affinity Fe(2+) transport protein (Low-affinity Fe(II) transport protein) PROTEIN FAMILY: FET4 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the FET4 family. FUNCTION: Required for Fe(2+) ion low affinity uptake. KEYWORDS: Ion transport;Iron;Iron transport;Isopeptide bond;Membrane;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix;Transport;Ubl conjugation SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. MISCELLANEOUS: Present with 573 molecules/cell in log phase SD medium. P40993,PROTEIN NAMES: Ribonuclease MRP protein subunit SNM1 (RNA-processing protein SNM1) (RNase MRP 22.5 kDa subunit) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Essential component of the MRP ribonucleoprotein endoribonuclease that cleaves mitochondrial primer RNA sequences. KEYWORDS: 3D-structure;Hydrolase;Nuclease;Nucleus;Reference proteome;RNA-binding;rRNA processing SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: Present with 172 molecules/cell in log phase SD medium. P40995,PROTEIN NAMES: Rho guanine nucleotide exchange factor scd1 ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) FUNCTION: Required for mating and morphogenesis. May contain a cryptic binding site for cdc42 that is enhanced by binding Ras. Interacts directly with scd2. Promotes the exchange of cdc42-bound GDP by GTP. Involved in septation and stimulates the elongation of conjugation tubes. KEYWORDS: Cell cycle;Cell division;Cytoplasm;Guanine-nucleotide releasing factor;Nucleus;Phosphoprotein;Reference proteome;Septation SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=Located at the growing cell ends and tips of conjugation tubes. P40996,PROTEIN NAMES: Protein scd2/ral3 ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) FUNCTION: Required for mating and morphogenesis. Interacts directly with scd1 and with cdc42. May bridge and facilitate scd1 and cdc42 interactions. KEYWORDS: Reference proteome;Repeat;SH3 domain P41034,"PROTEIN NAMES: Alpha-tocopherol transfer protein (Alpha-TTP) ORGANISM: Rattus norvegicus (Rat) FUNCTION: Binds alpha-tocopherol, enhances its transfer between separate membranes, and stimulates its release from liver cells. Binds both phosphatidylinositol 3,4-bisphosphate and phosphatidylinositolphosphatidylinol 4,5-bisphosphate; the resulting conformation change is important for the release of the bound alpha-tocopherol (By similarity). KEYWORDS: Cytoplasm;Direct protein sequencing;Lipid-binding;Reference proteome;Transport SUBCELLULAR LOCATION: Cytoplasm. " P41073,"PROTEIN NAMES: Zinc finger protein on ecdysone puffs ORGANISM: Drosophila melanogaster (Fruit fly) FUNCTION: May play a role in the process of early and late gene activation, or possibly in RNA processing, for a defined set of developmentally regulated loci. KEYWORDS: Alternative splicing;Chromosome;DNA-binding;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Repeat;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Chromosome Note=It is associated with the active ecdysone-regulated loci on polytene chromosomes, and on some heat shock-induced puffs. Its distribution pattern follows the changes of puffing patterns in the developmental program, or following heat shock. " P41151,"PROTEIN NAMES: Heat stress transcription factor A-1a (AtHsfA1a) (AtHsf-13) (Heat shock factor protein 1) (HSF 1) (Heat shock transcription factor 1) (HSTF 1) PROTEIN FAMILY: HSF family, Class A subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the HSF family. Class A subfamily. FUNCTION: Transcriptional activator that specifically binds DNA sequence 5'-AGAAnnTTCT-3' known as heat shock promoter elements (HSE). KEYWORDS: Activator;Cytoplasm;DNA-binding;Nucleus;Phosphoprotein;Reference proteome;Stress response;Transcription;Transcription regulation SUBCELLULAR LOCATION: Cytoplasm Nucleus. " P41161,PROTEIN NAMES: ETS translocation variant 5 (Ets-related protein ERM) PROTEIN FAMILY: ETS family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the ETS family. FUNCTION: Binds to DNA sequences containing the consensus nucleotide core sequence 5'-GGAA.-3'. KEYWORDS: 3D-structure;Alternative splicing;DNA-binding;Isopeptide bond;Nucleus;Phosphoprotein;Reference proteome;Ubl conjugation SUBCELLULAR LOCATION: Nucleus. P41225,PROTEIN NAMES: Transcription factor SOX-3 ORGANISM: Homo sapiens (Human) FUNCTION: Transcription factor required during the formation of the hypothalamo-pituitary axis. May function as a switch in neuronal development. Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation. Required also within the pharyngeal epithelia for craniofacial morphogenesis. Controls a genetic switch in male development. Is necessary for initiating male sex determination by directing the development of supporting cell precursors (pre-Sertoli cells) as Sertoli rather than granulosa cells (By similarity). KEYWORDS: Developmental protein;Disease variant;DNA-binding;Intellectual disability;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus. P41247,"PROTEIN NAMES: Patatin-like phospholipase domain-containing protein 4 (Calcium-independent phospholipase A2-eta) (iPLA2-eta) (Protein GS2) ORGANISM: Homo sapiens (Human) FUNCTION: Has abundant triacylglycerol lipase activity. Transfers fatty acid from triglyceride to retinol, hydrolyzes retinylesters, and generates 1,3-diacylglycerol from triglycerides. Additionally possesses acylglycerol transacylase and phospholipase A2 activities. KEYWORDS: Alternative splicing;Hydrolase;Lipid degradation;Lipid metabolism;Mitochondrion;Primary mitochondrial disease;Reference proteome SUBCELLULAR LOCATION: Mitochondrion " P41273,PROTEIN NAMES: Tumor necrosis factor ligand superfamily member 9 (4-1BB ligand) (4-1BBL) PROTEIN FAMILY: Tumor necrosis factor family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the tumor necrosis factor family. FUNCTION: Cytokine that binds to TNFRSF9. Induces the proliferation of activated peripheral blood T-cells. May have a role in activation-induced cell death (AICD). May play a role in cognate interactions between T-cells and B-cells/macrophages. KEYWORDS: 3D-structure;Cytokine;Membrane;Reference proteome;Signal-anchor;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane; Single-pass type II membrane protein. P41274,PROTEIN NAMES: Tumor necrosis factor ligand superfamily member 9 (4-1BB ligand) (4-1BBL) PROTEIN FAMILY: Tumor necrosis factor family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the tumor necrosis factor family. FUNCTION: Cytokine that binds to TNFRSF9. Induces the proliferation of activated peripheral blood T-cells. May have a role in activation-induced cell death (AICD). May play a role in cognate interactions between T-cells and B-cells/macrophages. KEYWORDS: 3D-structure;Cytokine;Glycoprotein;Membrane;Reference proteome;Signal-anchor;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane; Single-pass type II membrane protein. P41279,"PROTEIN NAMES: Mitogen-activated protein kinase kinase kinase 8 (Cancer Osaka thyroid oncogene) (Proto-oncogene c-Cot) (Serine/threonine-protein kinase cot) (Tumor progression locus 2) (TPL-2) PROTEIN FAMILY: Protein kinase superfamily, STE Ser/Thr protein kinase family, MAP kinase kinase kinase subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily. FUNCTION: Required for lipopolysaccharide (LPS)-induced, TLR4-mediated activation of the MAPK/ERK pathway in macrophages, thus being critical for production of the pro-inflammatory cytokine TNF-alpha (TNF) during immune responses. Involved in the regulation of T-helper cell differentiation and IFNG expression in T-cells. Involved in mediating host resistance to bacterial infection through negative regulation of type I interferon (IFN) production. In vitro, activates MAPK/ERK pathway in response to IL1 in an IRAK1-independent manner, leading to up-regulation of IL8 and CCL4. Transduces CD40 and TNFRSF1A signals that activate ERK in B-cells and macrophages, and thus may play a role in the regulation of immunoglobulin production. May also play a role in the transduction of TNF signals that activate JNK and NF-kappa-B in some cell types. In adipocytes, activates MAPK/ERK pathway in an IKBKB-dependent manner in response to IL1B and TNF, but not insulin, leading to induction of lipolysis. Plays a role in the cell cycle. Isoform 1 shows some transforming activity, although it is much weaker than that of the activated oncogenic variant. KEYWORDS: 3D-structure;Alternative initiation;ATP-binding;Cell cycle;Cytoplasm;Immunity;Kinase;Magnesium;Metal-binding;Nucleotide-binding;Phosphoprotein;Proto-oncogene;Reference proteome;Serine/threonine-protein kinase;Transferase SUBCELLULAR LOCATION: Cytoplasm MISCELLANEOUS: Can be converted to an oncogenic protein by proviral activation, leading to a C-terminally truncated protein with transforming activity." P41391,"PROTEIN NAMES: Ran GTPase-activating protein 1 (Protein rna1) PROTEIN FAMILY: RNA1 family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the RNA1 family. FUNCTION: GTPase activator for the nuclear Ras-related regulatory protein spi1 (Ran), converting it to the putatively inactive GDP-bound state. KEYWORDS: 3D-structure;Cytoplasm;Direct protein sequencing;GTPase activation;Leucine-rich repeat;Reference proteome;Repeat SUBCELLULAR LOCATION: Cytoplasm. Cytoplasm, perinuclear region Note=Possibly enriched in the nuclear periphery. " P41411,"PROTEIN NAMES: Cell division control protein 18 PROTEIN FAMILY: CDC6/cdc18 family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the CDC6/cdc18 family. FUNCTION: Part of the checkpoint control that prevents mitosis from occurring until S phase is completed. Plays a key role in coupling S phase to start and mitosis. Acts at the initiation of DNA replication and plays a major role in controlling the onset of S-phase. Together with orc1, involved in the maintenance of replication forks and activation of cds1-dependent S-phase checkpoint. Together with orc2, plays a role in preventing DNA rereplication and resulting genetic instability. KEYWORDS: ATP-binding;Cell cycle;Cell division;DNA replication;Mitosis;Nucleotide-binding;Reference proteome;Ubl conjugation " P41412,PROTEIN NAMES: Cell division cycle-related protein res2/pct1 ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) FUNCTION: Required for the initiation of mitotic and premeiotic DNA synthesis. Has an additional role in meiotic division. KEYWORDS: ANK repeat;Cell cycle;DNA-binding;Meiosis;Reference proteome;Repeat P41543,"PROTEIN NAMES: Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 (N-linked oligosaccharyl transferase subunit 1) (Oligosaccharyl transferase 64 kDa subunit) (Oligosaccharyl transferase subunit OST1) (Oligosaccharyl transferase subunit alpha) PROTEIN FAMILY: OST1 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the OST1 family. FUNCTION: Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity. PATHWAY: Protein modification; protein glycosylation. KEYWORDS: 3D-structure;Direct protein sequencing;Endoplasmic reticulum;Glycoprotein;Membrane;Reference proteome;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Single-pass type I membrane protein MISCELLANEOUS: Present with 11900 molecules/cell in log phase SD medium." P41596,"PROTEIN NAMES: Dopamine receptor 1 (D-DOP1) (DmDop1) (dDA1) (Dopamine 1-like receptor 1) PROTEIN FAMILY: G-protein coupled receptor 1 family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the G-protein coupled receptor 1 family. FUNCTION: Receptor for dopamine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. Might be involved in the processing of visual information and/or visual learning. Important for Pavlovian conditioning: required in the mushroom body as a receptor conveying unconditional stimuli information, has a role in memory formation for aversive and appetitive learning. Sleep-deprivation-induced impairments in learning can be partially explained through alterations in dopamine signaling, Dop1R1 expression levels are reduced; sleep may have a role in restoring dopamine homeostasis. KEYWORDS: Alternative splicing;Cell membrane;Disulfide bond;G-protein coupled receptor;Glycoprotein;Lipoprotein;Membrane;Palmitate;Phosphoprotein;Receptor;Reference proteome;Signal;Transducer;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. MISCELLANEOUS: Potency of neurotransmitter agonists in stimulating cAMP production and the lack of stimulation by other transmitters and metabolites suggests this is a D1-like receptor. Low homology to vertebrate D1 receptors suggests this may be a progenitor of the D1 receptor subfamily." P41739,"PROTEIN NAMES: Aryl hydrocarbon receptor nuclear translocator (ARNT protein) (Dioxin receptor, nuclear translocator) (Hypoxia-inducible factor 1-beta) (HIF-1-beta) (HIF1-beta) ORGANISM: Rattus norvegicus (Rat) FUNCTION: Required for activity of the AHR. Upon ligand binding, AHR translocates into the nucleus, where it heterodimerizes with ARNT and induces transcription by binding to xenobiotic response elements (XRE). Not required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex initiates transcription of genes involved in the regulation of a variety of biological processes, including angiogenesis, hematopoiesis, drug and lipid metabolism, cell motility and immune modulation (By similarity). The heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters and functions as a transcriptional regulator of the adaptive response to hypoxia (By similarity). The heterodimer ARNT:AHR binds to core DNA sequence 5'-TGCGTG-3' within the dioxin response element (DRE) of target gene promoters and activates their transcription (By similarity). KEYWORDS: Acetylation;Activator;DNA-binding;Isopeptide bond;Nucleus;Phosphoprotein;Reference proteome;Repeat;Transcription;Transcription regulation;Ubl conjugation SUBCELLULAR LOCATION: Nucleus " P41772,PROTEIN NAMES: Metal-activated transcriptional activator protein AMT1 ORGANISM: Candida glabrata (strain ATCC 2001 / BCRC 20586 / JCM 3761 / NBRC 0622 / NRRL Y-65 / CBS 138) (Yeast) (Nakaseomyces glabratus) FUNCTION: Trans-acting regulatory protein that activates transcription of the MT genes (metallothionein) in response to copper or silver ions. KEYWORDS: 3D-structure;Activator;Copper;DNA-binding;Metal-binding;Nucleus;Reference proteome;Transcription;Transcription regulation;Zinc SUBCELLULAR LOCATION: Nucleus. P41816,"PROTEIN NAMES: NADPH dehydrogenase 3 (Old yellow enzyme 3) PROTEIN FAMILY: NADH:flavin oxidoreductase/NADH oxidase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family. FUNCTION: Flavin-dependent enoate reductase that catalyzes the chemo- and stereoslective hydrogenation of electron-poor alkenes. The enzyme is reduced by NADPH, and oxygen, quinones, and alpha,beta-unsaturated aldehydes and ketones can act as electron acceptors to complete catalytic turnover. The physiological oxidant remains elusive (By similarity). Has a prooxidant activity, increasing reactive oxygen species (ROS) levels when overexpressed. Formation of OYE2-OYE3 heterodimers contribute to the induction of programmed cell death upon oxidative stress. KEYWORDS: 3D-structure;Direct protein sequencing;Flavoprotein;FMN;NADP;Oxidoreductase;Reference proteome " P41830,"PROTEIN NAMES: Steroid hormone receptor family member cnr14 (Nuclear receptor subfamily 1 group G member 1) PROTEIN FAMILY: Nuclear hormone receptor family, NR1 subfamily ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the nuclear hormone receptor family. NR1 subfamily. FUNCTION: Transcriptional regulator which is involved in the sex determination and X chromosome dosage compensation pathways. Directly binds to five 5'-A(G/C)(G/T)(T/G)C(A/G)-3' sites in the promoter of sex-determining factor xol-1 to negatively regulate its expression and promote hermaphrodite development. Together with fox-1 is involved in making the distinction between one and two X-chromosomes. Plays a role in the fox-1-mediated repression of the functionally active isoform (isoform b) of the sex-determining factor xol-1 gene to promote hermaphrodite development. Plays a role in the association of the dosage compensation complex proteins dpy-27 and sdc-3 with the hermaphrodite X chromosomes. KEYWORDS: Differentiation;DNA-binding;Metal-binding;Nucleus;Receptor;Reference proteome;Sexual differentiation;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus " P41834,PROTEIN NAMES: Syntaxin UFE1 PROTEIN FAMILY: Syntaxin family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the syntaxin family. FUNCTION: Syntaxin required for targeting and fusion of Golgi-derived retrograde transport vesicles with the ER. KEYWORDS: Coiled coil;Endoplasmic reticulum;ER-Golgi transport;Membrane;Protein transport;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Single-pass type IV membrane protein. P41855,"PROTEIN NAMES: FMRFamide-like neuropeptides 1 [Cleaved into: PNFMRY-amide; AGSDPNFLRF-amide; SQPNFLRF-amide; ASGDPNFLRF-amide; SDPNFLRF-amide (PF1); AAADPNFLRF-amide; SADPNFLRF-amide (PF2); PNFLRF-amide] PROTEIN FAMILY: FARP (FMRFamide related peptide) family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the FARP (FMRFamide related peptide) family. FUNCTION: Together with flp-18, plays a homeostatic role by acting on the GABAergic neural transmission at neuromuscular junctions to prevent overexcitation of the locomotor circuit.; FUNCTION: [SADPNFLRF-amide]: Inhibits the activity of dissected pharyngeal myogenic muscle system.; FUNCTION: DPNFLRF-amide: Inhibits the activity of dissected pharyngeal myogenic muscle system.; FUNCTION: [PNFMRY-amide]: Acts as a ligand for the npr-22 receptor in vitro. KEYWORDS: Alternative splicing;Amidation;Cleavage on pair of basic residues;Direct protein sequencing;Neuropeptide;Reference proteome;Repeat;Secreted;Signal SUBCELLULAR LOCATION: Secreted. MISCELLANEOUS: [Isoform b]: Expressed at about a twofold higher level than isoform Long." P41896,"PROTEIN NAMES: Transcription initiation factor IIF subunit beta (TFIIF medium subunit) (TFIIF-beta) (Transcription factor G 54 kDa subunit) PROTEIN FAMILY: TFIIF beta subunit family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the TFIIF beta subunit family. FUNCTION: TFIIF is a general transcription initiation factor that binds to RNA polymerase II. Its functions include the recruitment of RNA polymerase II to the promoter bound DNA-TBP-TFIIB complex, decreasing the affinity of RNA polymerase II for non-specific DNA, allowing for the subsequent recruitment of TFIIE and TFIIH, and facilitating RNA polymerase II elongation. KEYWORDS: 3D-structure;Direct protein sequencing;DNA-binding;Nucleotide-binding;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus. MISCELLANEOUS: Present with 520 molecules/cell in log phase SD medium." P41901,"PROTEIN NAMES: Sporulation-regulated protein 3 PROTEIN FAMILY: TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily, Septin GTPase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family. FUNCTION: Septins are GTPases involved in cytokinesis that assemble into filaments and form a ring at the cleavage site. May act by recruiting MYO1 and HOF1, a protein involved in septation, to the site of cleavage. Septins are also involved in cell morphogenesis, bud site selection, chitin deposition, cell cycle regulation, cell compartmentalization and spore wall formation (By similarity). KEYWORDS: Coiled coil;GTP-binding;Membrane;Nucleotide-binding;Reference proteome;Sporulation SUBCELLULAR LOCATION: Prospore membrane ; Peripheral membrane protein Bud neck Note=Present at the bud neck during cell division. Probably interacts with phosphoinosides such as phosphatidylinositol 4-phosphate or phosphatidylinositol 5-phosphate (By similarity). Localized to the leading edges of the membrane sacs that form near the spindle-pole bodies and gradually extend to engulf the nuclear lobes that contain the haploid chromosome sets, thus forming the spores. " P41909,"PROTEIN NAMES: Peroxisomal long-chain fatty acid import protein 2 (Peroxisomal ABC transporter 1) PROTEIN FAMILY: ABC transporter superfamily, ABCD family, Peroxisomal fatty acyl CoA transporter subfamily ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the ABC transporter superfamily. ABCD family. Peroxisomal fatty acyl CoA transporter (TC 3.A.1.203) subfamily. FUNCTION: Involved in the import of activated long-chain fatty acids from the cytosol to the peroxisomal matrix. KEYWORDS: ATP-binding;Glycoprotein;Membrane;Nucleotide-binding;Peroxisome;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Peroxisome membrane; Multi-pass membrane protein. " P41911,"PROTEIN NAMES: Glycerol-3-phosphate dehydrogenase [NAD(+)] 2, mitochondrial PROTEIN FAMILY: NAD-dependent glycerol-3-phosphate dehydrogenase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family. FUNCTION: Catalyzes the production of glycerol under anaerobic growth conditions. Glycerol production serves as a redox sink by consuming the excess cytosolic NADH during anaerobic metabolism. KEYWORDS: Cytoplasm;Mitochondrion;NAD;Oxidoreductase;Phosphoprotein;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Cytoplasm. Mitochondrion. MISCELLANEOUS: Present with 8966 molecules/cell in log phase SD medium." P41934,"PROTEIN NAMES: Homeobox protein ceh-18 PROTEIN FAMILY: POU transcription factor family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the POU transcription factor family. FUNCTION: Directs gonadal sheath cell differentiation and function. Also directs gonad migration and plays a role in specifying the differentiated phenotypes of epidermal cells during postembryonic development. Plays a role in oogenesis, regulating a sheath cell signal that causes oocytes to maintain diakinesis arrest during meiosis. Negatively regulates oocyte maturation, ovulation and MAPK activation in oocytes when sperm are not available for fertilization. May be recruited by akir-1 to the promoter regions of antimicrobial peptide genes to control gene expression in response to fungal infection. KEYWORDS: Developmental protein;DNA-binding;Homeobox;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus " P41936,PROTEIN NAMES: Homeobox protein ceh-22 PROTEIN FAMILY: NK-2 homeobox family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the NK-2 homeobox family. FUNCTION: Involved in combinatorial activation of gene expression in pharyngeal muscle. Specifically binds a site necessary for activity of the B subelement of myo-2 enhancer.; FUNCTION: [Isoform b]: Regulates distal tip cell fate. KEYWORDS: Alternative splicing;Developmental protein;DNA-binding;Homeobox;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus. P41990,"PROTEIN NAMES: Anterior pharynx in excess protein 1 ORGANISM: Caenorhabditis elegans FUNCTION: Probable ligand for lin-12/Notch and glp-1/Notch receptors and involved in the mediation of Notch signaling. Involved in the lin-12/Notch pathway signaling of cell fate in vulval precursor cells (VPCs), acting redundantly with dsl-1 and lag-2. Contributes to the establishment of the dorsal-ventral axis in early embryos. Involved in the specification of the blastomere cell ABp fate, probably acting as a signal from the P2 blastomere to the glp-1/Notch receptor on ABp and ABa. Probably acts as a signal, from the secondary vulval epithelial cells and the vulval muscle type 1 (vm1) cells, to activate the lin-12/Notch pathway in type 2 vulval muscle (vm2) cells, contributing to formation of the postsynaptic muscle plasma membrane extensions, known as muscle arms. Required for oocyte growth control, acting redundantly with lag-2, perhaps signaling via the glp-1/Notch pathway. Plays a somatic role in ovulation during adulthood, perhaps via lin-12/Notch signaling. Involved in establishing left-right asymmetry during intestinal organogenesis. KEYWORDS: Cell membrane;Cytoplasm;Developmental protein;Differentiation;Disulfide bond;EGF-like domain;Glycoprotein;Membrane;Notch signaling pathway;Nucleus;Reference proteome;Repeat;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein Nucleus Cytoplasm Note=Nuclear localization of transcripts at 36-cell stage in embryos. " P42124,"PROTEIN NAMES: Histone-lysine N-methyltransferase E(z) (Lysine N-methyltransferase 6) (Protein enhancer of zeste) PROTEIN FAMILY: Class V-like SAM-binding methyltransferase superfamily, Histone-lysine methyltransferase family, EZ subfamily ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. EZ subfamily. FUNCTION: Polycomb group (PcG) protein. Catalytic subunit of the Esc/E(z) complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. While PcG proteins are generally required to maintain the transcriptionally repressive state of homeotic genes throughout development, this protein is specifically required during the first 6 hours of embryogenesis to establish the repressed state. The Esc/E(z) complex is necessary but not sufficient for the repression of homeotic target genes, suggesting that the recruitment of the distinct PRC1 complex is also required. KEYWORDS: Chromatin regulator;Developmental protein;Methyltransferase;Nucleus;Phosphoprotein;Reference proteome;Repressor;S-adenosyl-L-methionine;Transcription;Transcription regulation;Transferase SUBCELLULAR LOCATION: Nucleus " P42166,"PROTEIN NAMES: Lamina-associated polypeptide 2, isoform alpha (Thymopoietin isoform alpha) (TP alpha) (Thymopoietin-related peptide isoform alpha) (TPRP isoform alpha) [Cleaved into: Thymopoietin (TP) (Splenin); Thymopentin (TP5)] PROTEIN FAMILY: LEM family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the LEM family. FUNCTION: May be involved in the structural organization of the nucleus and in the post-mitotic nuclear assembly. Plays an important role, together with LMNA, in the nuclear anchorage of RB1.; FUNCTION: TP and TP5 may play a role in T-cell development and function. TP5 is an immunomodulating pentapeptide. KEYWORDS: 3D-structure;Acetylation;Alternative splicing;Cardiomyopathy;Chromosome;Coiled coil;Direct protein sequencing;DNA-binding;Methylation;Nucleus;Pharmaceutical;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Nucleus. Chromosome. Note=Expressed diffusely throughout the nucleus. " P42212,PROTEIN NAMES: Green fluorescent protein PROTEIN FAMILY: GFP family ORGANISM: Aequorea victoria (Water jellyfish) (Mesonema victoria) SIMILARITY: Belongs to the GFP family. FUNCTION: Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin. KEYWORDS: 3D-structure;Chromophore;Direct protein sequencing;Luminescence;Photoprotein P42236,"PROTEIN NAMES: Alpha-ketoglutaric semialdehyde dehydrogenase (alphaKGSA dehydrogenase) (2,5-dioxovalerate dehydrogenase) PROTEIN FAMILY: Aldehyde dehydrogenase family ORGANISM: Bacillus subtilis (strain 168) SIMILARITY: Belongs to the aldehyde dehydrogenase family. FUNCTION: Catalyzes the NAD(P)(+)-dependent oxidation of alpha-ketoglutaric semialdehyde (alphaKGSA) to alpha-ketoglutarate. Prefers NADP(+) to NAD(+) as a cosubstrate. In vitro, can also use various aldehydes. KEYWORDS: NAD;NADP;Oxidoreductase;Reference proteome " P42282,"PROTEIN NAMES: Protein tramtrack, alpha isoform (Repressor protein fushi tarazu) (Tramtrack p88) ORGANISM: Drosophila melanogaster (Fruit fly) FUNCTION: Binds to a number of sites in the transcriptional regulatory region of ftz. Isoform alpha is required to repress genes that promote the R7 cell fate. Probable repressor of the transcription of the segmentation genes ftz, eve, h, odd, run, and en. May bind to the region 5'-AGGG[CT]GG-3'. Degradation of ttk is directed by binding of sinah or sina, via the adapter molecule phyl which binds to the BTB domain of ttk. KEYWORDS: Alternative splicing;DNA-binding;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Repeat;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus " P42292,"PROTEIN NAMES: CD166 antigen (Activated leukocyte cell adhesion molecule) (BEN glycoprotein) (Protein DM-GRASP) (Protein JC7) (SC1 glycoprotein) (CD antigen CD166) ORGANISM: Gallus gallus (Chicken) FUNCTION: Cell adhesion molecule that mediates both heterotypic cell-cell contacts via its interaction with CD6, as well as homotypic cell-cell contacts. Promotes T-cell activation and proliferation via its interactions with CD6 (By similarity). Contributes to the formation and maturation of the immunological synapse via its interactions with CD6 (By similarity). Mediates homotypic interactions with cells that express ALCAM. Mediates attachment of dendritic cells onto endothelial cells via homotypic interaction. Inhibits endothelial cell migration and promotes endothelial tube formation via homotypic interactions. Required for normal organization of the lymph vessel network. Required for normal hematopoietic stem cell engraftment in the bone marrow. Plays a role in hematopoiesis; required for normal numbers of hematopoietic stem cells in bone marrow. Promotes in vitro osteoblast proliferation and differentiation (By similarity). Promotes neurite extension, axon growth and axon guidance; axons grow preferentially on surfaces that contain ALCAM. Mediates outgrowth and pathfinding for retinal ganglion cell axons. KEYWORDS: Adaptive immunity;Cell adhesion;Cell membrane;Cell projection;Direct protein sequencing;Disulfide bond;Glycoprotein;Immunity;Immunoglobulin domain;Membrane;Reference proteome;Repeat;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein Cell projection, axon Cell projection, dendrite Note=Detected at the immunological synapse, i.e, at the contact zone between antigen-presenting dendritic cells and T-cells. Colocalizes with CD6 and the TCR/CD3 complex at the immunological synapse. " P42517,PROTEIN NAMES: Monofunctional chorismate mutase (CM-F) ORGANISM: Enterobacter agglomerans (Erwinia herbicola) (Pantoea agglomerans) FUNCTION: Catalyzes the Claisen rearrangement of chorismate to prephenate. May sequester with cyclohexadienyl dehydratase and an aromatic aminotransferase to form phenylalanine or phenylpyruvate. PATHWAY: Metabolic intermediate biosynthesis; prephenate biosynthesis; prephenate from chorismate: step 1/1. KEYWORDS: Amino-acid biosynthesis;Aromatic amino acid biosynthesis;Direct protein sequencing;Isomerase;Periplasm;Phenylalanine biosynthesis;Signal SUBCELLULAR LOCATION: Periplasm P42519,PROTEIN NAMES: Protein Star ORGANISM: Drosophila melanogaster (Fruit fly) FUNCTION: Involved in EGF receptor signaling. Has an early role in photoreceptor development. Interacts with the receptor torpedo in the eye. KEYWORDS: Cell membrane;Developmental protein;Endoplasmic reticulum;Golgi apparatus;Membrane;Reference proteome;Sensory transduction;Signal-anchor;Transmembrane;Transmembrane helix;Vision SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Single-pass type II membrane protein. Golgi apparatus membrane; Single-pass type II membrane protein. Cell membrane; Single-pass type II membrane protein. P42525,"PROTEIN NAMES: Extracellular signal-regulated kinase 1 (ERK1) (MAP kinase 1) PROTEIN FAMILY: Protein kinase superfamily, CMGC Ser/Thr protein kinase family, MAP kinase subfamily ORGANISM: Dictyostelium discoideum (Social amoeba) SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. FUNCTION: Kinase involved in a signal transduction pathway. KEYWORDS: ATP-binding;Cell cycle;Cell division;Kinase;Mitosis;Nucleotide-binding;Phosphoprotein;Reference proteome;Serine/threonine-protein kinase;Transferase " P42530,PROTEIN NAMES: Discoidin-2 (Discoidin II) ORGANISM: Dictyostelium discoideum (Social amoeba) FUNCTION: Galactose-binding lectin. May be necessary for the primary process of spore formation and may be involved in spore coat formation. KEYWORDS: 3D-structure;Calcium;Cell adhesion;Direct protein sequencing;Lectin;Metal-binding;Phosphoprotein;Reference proteome P42565,PROTEIN NAMES: FMRFamide-related neuropeptides [Cleaved into: EFFPL-amide; GDPFLRF-amide 1; GDPFLRF-amide 2; GDPFLRF-amide 3; GDPFLRF-amide 4; GDPFLRF-amide 5; GDPFLRF-amide 6; GDPFLRF-amide 7; SDPFLRF-amide 1; SDPFLRF-amide 2; SDPFLRF-amide 3; SDPFLRF-amide 4; SDPFLRF-amide 5; SDPFLRF-amide 6; SDPYLRF-amide; SDPFFRF-amide; SKPYMRF-amide; SSFPRY-amide; HDYMRF-amide] PROTEIN FAMILY: FARP (FMRFamide related peptide) family ORGANISM: Lymnaea stagnalis (Great pond snail) (Helix stagnalis) SIMILARITY: Belongs to the FARP (FMRFamide related peptide) family. FUNCTION: SDPFLRF-amide inhibits neurons.; FUNCTION: SKPYMRF-amide excites neurons. KEYWORDS: Alternative splicing;Amidation;Cleavage on pair of basic residues;Direct protein sequencing;Neuropeptide;Repeat;Secreted;Signal SUBCELLULAR LOCATION: Secreted. P42567,"PROTEIN NAMES: Epidermal growth factor receptor substrate 15 (Protein Eps15) (Protein AF-1p) ORGANISM: Mus musculus (Mouse) FUNCTION: Involved in cell growth regulation. May be involved in the regulation of mitogenic signals and control of cell proliferation. Involved in the internalization of ligand-inducible receptors of the receptor tyrosine kinase (RTK) type, in particular EGFR. Plays a role in the assembly of clathrin-coated pits (CCPs). Acts as a clathrin adapter required for post-Golgi trafficking. Seems to be involved in CCPs maturation including invagination or budding. Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR); internalization of ITGB1 as DAB2-dependent cargo but not TFR seems to require association with DAB2. KEYWORDS: 3D-structure;Acetylation;Alternative splicing;Calcium;Cell membrane;Coated pit;Cytoplasm;Direct protein sequencing;Endocytosis;Endosome;Membrane;Metal-binding;Phosphoprotein;Protein transport;Reference proteome;Repeat;SH3-binding;Transport;Ubl conjugation SUBCELLULAR LOCATION: Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Membrane, clathrin-coated pit. Note=Recruited to the plasma membrane upon EGFR activation and localizes to coated pits. Colocalizes with UBQLN1 in ubiquitin-rich cytoplasmic aggregates that are not endocytic compartments and in cytoplasmic juxtanuclear structures called aggresomes.; SUBCELLULAR LOCATION: [Isoform 2]: Early endosome membrane ; Peripheral membrane protein ; Cytoplasmic side Note=Colocalizes with HGS on bilayered clathrin coats on endosomes. " P42679,"PROTEIN NAMES: Megakaryocyte-associated tyrosine-protein kinase (CSK homologous kinase) (CHK) (Hematopoietic consensus tyrosine-lacking kinase) (Protein kinase HYL) (Tyrosine-protein kinase CTK) PROTEIN FAMILY: Protein kinase superfamily, Tyr protein kinase family, CSK subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. CSK subfamily. FUNCTION: Could play a significant role in the signal transduction of hematopoietic cells. May regulate tyrosine kinase activity of SRC-family members in brain by specifically phosphorylating their C-terminal regulatory tyrosine residue which acts as a negative regulatory site. It may play an inhibitory role in the control of T-cell proliferation. KEYWORDS: 3D-structure;Alternative splicing;ATP-binding;Cytoplasm;Kinase;Membrane;Nucleotide-binding;Phosphoprotein;Reference proteome;SH2 domain;SH3 domain;Transferase;Tyrosine-protein kinase SUBCELLULAR LOCATION: Cytoplasm Membrane Note=In platelets, 90% of MATK localizes to the membrane fraction, and translocates to the cytoskeleton upon thrombin stimulation. " P42744,"PROTEIN NAMES: NEDD8-activating enzyme E1 regulatory subunit AXR1 (Auxin-resistance protein AXR1) (Protein AUXIN-RESISTANT 1) PROTEIN FAMILY: Ubiquitin-activating E1 family, ULA1 subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the ubiquitin-activating E1 family. ULA1 subfamily. FUNCTION: Regulatory subunit of the dimeric ECR1-AXR1 E1 enzyme. E1 activates RUB1/NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a RUB1-ECR1 thioester and free AMP. E1 finally transfers RUB1 to the catalytic cysteine of RCE1. Plays an important role in auxin response. Regulates the chromosomal localization of meiotic recombination by crossovers (COs) and subsequent synapsis, probably through the activation of a CRL4 complex. Required for E3-mediated protein degradation in response to auxin, jasmonic acid and cold stress. Required for the COP1-COP10-CSN-mediated repression of photomorphogenesis in the dark. May function redundantly with AXL1 in the RUB conjugating pathway. Seems not to be functionally equivalent to AXL1 in vivo. PATHWAY: Protein modification; protein neddylation. KEYWORDS: Alternative splicing;Auxin signaling pathway;Nucleotide-binding;Nucleus;Reference proteome;Ubl conjugation pathway SUBCELLULAR LOCATION: Nucleus " P42770,"PROTEIN NAMES: Glutathione reductase, chloroplastic (GR) (GRase) (Protein EMBRYO DEFECTIVE 2360) PROTEIN FAMILY: Class-I pyridine nucleotide-disulfide oxidoreductase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family. FUNCTION: Maintains high levels of reduced glutathione in the chloroplast. KEYWORDS: Chloroplast;Disulfide bond;FAD;Flavoprotein;NADP;Oxidoreductase;Plastid;Redox-active center;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast MISCELLANEOUS: The active site is a redox-active disulfide bond." P42820,"PROTEIN NAMES: Acidic endochitinase SP2 PROTEIN FAMILY: Glycosyl hydrolase 19 family, Chitinase class I subfamily ORGANISM: Beta vulgaris (Sugar beet) SIMILARITY: Belongs to the glycosyl hydrolase 19 family. Chitinase class I subfamily. FUNCTION: Defense against chitin-containing fungal pathogens. KEYWORDS: Carbohydrate metabolism;Chitin degradation;Chitin-binding;Direct protein sequencing;Disulfide bond;Glycoprotein;Glycosidase;Hydrolase;Hydroxylation;Plant defense;Polysaccharide degradation;Pyrrolidone carboxylic acid;Repeat;Secreted;Signal SUBCELLULAR LOCATION: Secreted, extracellular space. " P42867,"PROTEIN NAMES: UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase (GlcNAc-1-P transferase) (G1PT) (GPT) (N-acetylglucosamine-1-phosphate transferase) PROTEIN FAMILY: Glycosyltransferase 4 family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the glycosyltransferase 4 family. FUNCTION: UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. Catalyzes the initial step of dolichol-linked oligosaccharide biosynthesis, transfering GlcNAc-1-P from cytosolic UDP-GlcNAc onto the carrier lipid dolichyl phosphate (P-dolichol), yielding GlcNAc-P-P-dolichol embedded in the cytoplasmic leaflet of the endoplasmic reticulum membrane. PATHWAY: Protein modification; protein glycosylation. KEYWORDS: 3D-structure;Endoplasmic reticulum;Glycoprotein;Glycosyltransferase;Magnesium;Membrane;Metal-binding;Reference proteome;RNA editing;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein " P42951,PROTEIN NAMES: Phosphatidylinositol 4-kinase LSB6 (PI4-kinase) (PtdIns-4-kinase) (LAS seventeen-binding protein 6) (LAS17-binding protein 6) PROTEIN FAMILY: PI3/PI4-kinase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the PI3/PI4-kinase family. FUNCTION: May play a role in endocytic and/or exocytic pathways. KEYWORDS: ATP-binding;Cell membrane;Kinase;Magnesium;Manganese;Membrane;Metal-binding;Nucleotide-binding;Reference proteome;Transferase;Vacuole SUBCELLULAR LOCATION: Cell membrane ; Peripheral membrane protein Vacuole membrane ; Peripheral membrane protein Note=Located at both the plasma and vacuolar membrane. MISCELLANEOUS: Present with 56 molecules/cell in log phase SD medium. P42971,"PROTEIN NAMES: Penicillin-binding protein 3 (PBP 3) (PSPB20) (Penicillin-binding protein C) PROTEIN FAMILY: Transpeptidase family ORGANISM: Bacillus subtilis (strain 168) SIMILARITY: Belongs to the transpeptidase family. FUNCTION: Penicillin-binding proteins (PBPs) function in the late steps of murein biosynthesis (Probable). Probably required for both cortical and vegetative peptidoglycan synthesis (Probable). Although not usually required for cell division, in the absence of PBP 2B (pbpB) it becomes essential. Confers resistance to oxacillin and cephalexin. PATHWAY: Cell wall biogenesis; peptidoglycan biosynthesis. KEYWORDS: Antibiotic resistance;Carboxypeptidase;Cell membrane;Cell shape;Cell wall biogenesis/degradation;Direct protein sequencing;Hydrolase;Membrane;Peptidoglycan synthesis;Protease;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass membrane protein Forespore inner membrane ; Single-pass membrane protein Forespore outer membrane ; Single-pass membrane protein. Membrane raft ; Single-pass membrane protein Note=Localizes at mid cell; localization requires FtsZ and PBP 2B (pbpB). Present in detergent-resistant membrane (DRM) fractions that may be equivalent to eukaryotic membrane rafts; these rafts include proteins involved in signaling, molecule trafficking and protein secretion. " P43029,PROTEIN NAMES: Growth/differentiation factor 7 (GDF-7) PROTEIN FAMILY: TGF-beta family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the TGF-beta family. KEYWORDS: Alternative splicing;Cleavage on pair of basic residues;Cytokine;Disulfide bond;Glycoprotein;Growth factor;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted P43072,PROTEIN NAMES: Transcription factor IIIB 70 kDa subunit (TFIIIB) (B-related factor 1) (BRF-1) PROTEIN FAMILY: TFIIB family ORGANISM: Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) SIMILARITY: Belongs to the TFIIB family. FUNCTION: General activator of RNA polymerase III transcription. Interacts with TBP. Binds to Pol III subunit C34 and to the TAU135 component of TFIIIC. KEYWORDS: Activator;Metal-binding;Nucleus;Reference proteome;Repeat;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus. P43094,"PROTEIN NAMES: Secreted aspartic protease 5 (ACP 5) (Aspartate protease 5) (Candidapepsin-5) PROTEIN FAMILY: Peptidase A1 family ORGANISM: Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) SIMILARITY: Belongs to the peptidase A1 family. FUNCTION: Secreted aspartic peptidases (SAPs) are a group of ten acidic hydrolases considered as key virulence factors. These enzymes supply the fungus with nutrient amino acids as well as are able to degrade the selected host's proteins involved in the immune defense. Moreover, acts toward human hemoglobin though limited proteolysis to generate a variety of antimicrobial hemocidins, enabling to compete with the other microorganisms of the same physiological niche using the microbicidal peptides generated from the host protein. KEYWORDS: 3D-structure;Aspartyl protease;Cleavage on pair of basic residues;Disulfide bond;Hydrolase;Metal-binding;Protease;Reference proteome;Secreted;Signal;Virulence;Zinc;Zymogen SUBCELLULAR LOCATION: Secreted " P43132,"PROTEIN NAMES: COMPASS component BRE2 (Brefeldin-A sensitivity protein 2) (COMPASS protein 60) (Complex proteins associated with SET1 protein BRE2) (Set1C component BRE2) PROTEIN FAMILY: CclA family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the cclA family. FUNCTION: Component of the Set1C/COMPASS complex that specifically mono-, di- and trimethylates histone H3 to form H3K4me1/2/3, which subsequently plays a role in telomere length maintenance and transcription elongation regulation. COMPASS recognizes ubiquitinated H2B on one face of the nucleosome which stimulates the methylation of H3 on the opposing face. KEYWORDS: 3D-structure;Chromosome;Nucleus;Phosphoprotein;Reference proteome;Telomere SUBCELLULAR LOCATION: Nucleus Chromosome, telomere MISCELLANEOUS: Present with 1310 molecules/cell in log phase SD medium." P43234,PROTEIN NAMES: Cathepsin O PROTEIN FAMILY: Peptidase C1 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the peptidase C1 family. FUNCTION: Proteolytic enzyme possibly involved in normal cellular protein degradation and turnover. KEYWORDS: Disulfide bond;Glycoprotein;Hydrolase;Lysosome;Protease;Reference proteome;Signal;Thiol protease;Zymogen SUBCELLULAR LOCATION: Lysosome. P43251,"PROTEIN NAMES: Biotinidase (Biotinase) PROTEIN FAMILY: Carbon-nitrogen hydrolase superfamily, BTD/VNN family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the carbon-nitrogen hydrolase superfamily. BTD/VNN family. FUNCTION: Catalytic release of biotin from biocytin, the product of biotin-dependent carboxylases degradation. KEYWORDS: Alternative splicing;Direct protein sequencing;Disease variant;Glycoprotein;Hydrolase;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted, extracellular space " P43255,"PROTEIN NAMES: COP9 signalosome complex subunit 8 (CSN complex subunit 8) (Constitutive photomorphogenesis protein 9) (Protein FUSCA 7) PROTEIN FAMILY: CSN8 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the CSN8 family. FUNCTION: Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes such as photomorphogenesis and auxin and jasmonate responses. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF. It is involved in repression of photomorphogenesis in darkness by regulating the activity of COP1-containing Ubl ligase complexes. The complex is also required for degradation of IAA6 by regulating the activity of the Ubl ligase SCF-TIR complex. KEYWORDS: Cytoplasm;Developmental protein;Nucleus;Phytochrome signaling pathway;Reference proteome;Signalosome SUBCELLULAR LOCATION: Cytoplasm Nucleus " P43303,"PROTEIN NAMES: Interleukin-1 receptor type 2 (IL-1R-2) (IL-1RT-2) (IL-1RT2) (CD121 antigen-like family member B) (IL-1 type II receptor) (Interleukin-1 receptor beta) (IL-1R-beta) (Interleukin-1 receptor type II) (CD antigen CD121b) [Cleaved into: Interleukin-1 receptor type 2, membrane form (mIL-1R2) (mIL-1RII); Interleukin-1 receptor type 2, soluble form (sIL-1R2) (sIL-1RII)] PROTEIN FAMILY: Interleukin-1 receptor family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the interleukin-1 receptor family. FUNCTION: Non-signaling receptor for IL1A, IL1B and IL1RN. Reduces IL1B activities. Serves as a decoy receptor by competitive binding to IL1B and preventing its binding to IL1R1. Also modulates cellular response through non-signaling association with IL1RAP after binding to IL1B. IL1R2 (membrane and secreted forms) preferentially binds IL1B and poorly IL1A and IL1RN. The secreted IL1R2 recruits secreted IL1RAP with high affinity; this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors (By similarity). KEYWORDS: Cell membrane;Disulfide bond;Glycoprotein;Immunoglobulin domain;Membrane;Receptor;Reference proteome;Repeat;Secreted;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. Cell membrane Secreted " P43432,"PROTEIN NAMES: Interleukin-12 subunit beta (IL-12B) (Cytotoxic lymphocyte maturation factor 40 kDa subunit) (CLMF p40) (IL-12 subunit p40) PROTEIN FAMILY: IL-12B family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the IL-12B family. FUNCTION: Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.; FUNCTION: Associates with IL23A to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of pro-inflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis. KEYWORDS: 3D-structure;Cytokine;Disulfide bond;Glycoprotein;Immunoglobulin domain;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted " P43490,"PROTEIN NAMES: Nicotinamide phosphoribosyltransferase (NAmPRTase) (Nampt) (Pre-B-cell colony-enhancing factor 1) (Pre-B cell-enhancing factor) (Visfatin) PROTEIN FAMILY: NAPRTase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the NAPRTase family. FUNCTION: Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. The secreted form behaves both as a cytokine with immunomodulating properties and an adipokine with anti-diabetic properties, it has no enzymatic activity, partly because of lack of activation by ATP, which has a low level in extracellular space and plasma. Plays a role in the modulation of circadian clock function. NAMPT-dependent oscillatory production of NAD regulates oscillation of clock target gene expression by releasing the core clock component: CLOCK-BMAL1 heterodimer from NAD-dependent SIRT1-mediated suppression (By similarity). PATHWAY: Cofactor biosynthesis; NAD(+) biosynthesis; nicotinamide D-ribonucleotide from 5-phospho-alpha-D-ribose 1-diphosphate and nicotinamide: step 1/1. KEYWORDS: 3D-structure;Acetylation;Biological rhythms;Cytokine;Cytoplasm;Glycosyltransferase;Nucleus;Phosphoprotein;Pyridine nucleotide biosynthesis;Reference proteome;Secreted;Transferase SUBCELLULAR LOCATION: Nucleus Cytoplasm Secreted Note=Under non-inflammatory conditions, visfatin predominantly exhibits a granular pattern within the nucleus. Secreted by endothelial cells upon IL-1beta stimulation. Abundantly secreted in milk, reaching 100-fold higher concentrations compared to maternal serum. " P43535,"PROTEIN NAMES: Protein GCN20 (General control non-derepressible protein 20) PROTEIN FAMILY: ABC transporter superfamily, ABCF family, EF3 subfamily ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the ABC transporter superfamily. ABCF family. EF3 subfamily. FUNCTION: Acts as a positive activator of the GCN2 protein kinase activity in response to in response to low amino acid, carbon, or purine availability. Component of the GCN1-GCN20 complex that forms a complex with GCN2 on translating ribosomes; during this process, GCN20 helps GCN1 to act as a chaperone to facilitate delivery of uncharged tRNAs that enter the A site of ribosomes to the tRNA-binding domain of GCN2, and hence stimulating GCN2 kinase activity. Participates in gene-specific mRNA translation activation, such as the transcriptional activator GCN4, by promoting the GCN2-mediated phosphorylation of eukaryotic translation initiation factor 2 (eIF-2-alpha/SUI2) on 'Ser-52', and hence allowing GCN4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion. KEYWORDS: Acetylation;Activator;ATP-binding;Nucleotide-binding;Reference proteome;Repeat;Stress response;Translation regulation MISCELLANEOUS: Present with 14600 molecules/cell in log phase SD medium." P43555,PROTEIN NAMES: Protein EMP47 (47 kDa endomembrane protein) (Endosomal P44 protein) PROTEIN FAMILY: EMP46/EMP47 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the EMP46/EMP47 family. FUNCTION: Involved in the secretion of glycoproteins and in nucleus architecture and gene silencing. Required for the endoplasmic reticulum exit of EMP46. KEYWORDS: 3D-structure;Direct protein sequencing;Disulfide bond;Endoplasmic reticulum;Golgi apparatus;Lectin;Membrane;Reference proteome;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. MISCELLANEOUS: Present with 2900 molecules/cell in log phase SD medium. P43558,PROTEIN NAMES: Ubiquitin thioesterase OTU1 (OTU domain-containing protein 1) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Hydrolase that can remove conjugated ubiquitin from proteins and may therefore play an important regulatory role at the level of protein turnover by preventing degradation. Participates in the regulation of the ubiquitin conjugation pathway involving CDC48 by hindering multiubiquitination of substrates at the CDC48 chaperone. May be indirectly involved in PIS1 gene expression. KEYWORDS: 3D-structure;Cytoplasm;Hydrolase;Isopeptide bond;Metal-binding;Nucleus;Protease;Reference proteome;Thiol protease;Ubl conjugation;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cytoplasm Nucleus MISCELLANEOUS: Present with 2770 molecules/cell in log phase SD medium. P43567,PROTEIN NAMES: Alanine--glyoxylate aminotransferase 1 PROTEIN FAMILY: Class-V pyridoxal-phosphate-dependent aminotransferase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. FUNCTION: Has alanine:glyoxylate aminotransferase activity. PATHWAY: Amino-acid biosynthesis; glycine biosynthesis; glycine from glyoxylate: step 1/1. KEYWORDS: 3D-structure;Aminotransferase;Pyridoxal phosphate;Reference proteome;Transferase MISCELLANEOUS: Present with 339 molecules/cell in log phase SD medium. Expression levels higher in stationary phase than in exponential growth phase when grown in complex medium with glucose. P43609,"PROTEIN NAMES: Chromatin structure-remodeling complex protein RSC8 (Remodel the structure of chromatin complex subunit 8) (SWI3 homolog) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Component of the chromatin structure-remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton. This subunit is essential for mitotic growth and for repression of CHA1 expression. KEYWORDS: 3D-structure;Chromatin regulator;Coiled coil;Direct protein sequencing;DNA-binding;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Note=Localizes to centromeric and flanking chromatin. Association with these loci is dependent on STH1. MISCELLANEOUS: Present with 3380 molecules/cell in log phase SD medium." P43618,"PROTEIN NAMES: Inner kinetochore subunit CNN1 (CENP-T homolog) (Co-purified with NNF1 protein 1) (Constitutive centromere-associated network protein CNN1) PROTEIN FAMILY: CENP-T/CNN1 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the CENP-T/CNN1 family. FUNCTION: Component of the kinetochore, a multiprotein complex that assembles on centromeric DNA and attaches chromosomes to spindle microtubules, mediating chromosome segregation and sister chromatid segregation during meiosis and mitosis. Component of the inner kinetochore constitutive centromere-associated network (CCAN), which serves as a structural platform for outer kinetochore assembly. CNN1 is important for the recruitment of the outer kinetochore Ndc80 complex. KEYWORDS: 3D-structure;Cell cycle;Cell division;Centromere;Chromosome;Kinetochore;Mitosis;Nucleus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Nucleus Chromosome, centromere, kinetochore MISCELLANEOUS: Present with 1300 molecules/cell in log phase SD medium." P43630,"PROTEIN NAMES: Killer cell immunoglobulin-like receptor 3DL2 (CD158 antigen-like family member K) (Natural killer-associated transcript 4) (NKAT-4) (p70 natural killer cell receptor clone CL-5) (p70 NK receptor CL-5) (CD antigen CD158k) PROTEIN FAMILY: Immunoglobulin superfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the immunoglobulin superfamily. FUNCTION: Receptor on natural killer (NK) cells and T cells for MHC class I molecules. Upon binding of peptide-free HLA-F open conformer, negatively regulates NK and T cell effector functions. Acts as a receptor on astrocytes for HLA-F. Through interaction with HLA-F, may protect motor neurons from astrocyte-induced toxicity. KEYWORDS: Alternative splicing;Cell membrane;Disulfide bond;Glycoprotein;Immunoglobulin domain;Membrane;Receptor;Reference proteome;Repeat;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. " P43633,"PROTEIN NAMES: Serine/threonine-protein kinase Haspin homolog ALK1 (DNA damage-responsive protein ALK1) PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family, Haspin subfamily ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Haspin subfamily. FUNCTION: Serine/threonine haspin-like protein kinase involved in cell cycle regulation. KEYWORDS: ATP-binding;Cell cycle;DNA damage;Kinase;Nucleotide-binding;Phosphoprotein;Reference proteome;Serine/threonine-protein kinase;Transferase MISCELLANEOUS: Present with 2190 molecules/cell in log phase SD medium." P43694,"PROTEIN NAMES: Transcription factor GATA-4 (GATA-binding factor 4) ORGANISM: Homo sapiens (Human) FUNCTION: Transcriptional activator that binds to the consensus sequence 5'-AGATAG-3' and plays a key role in cardiac development and function. In cooperation with TBX5, it binds to cardiac super-enhancers and promotes cardiomyocyte gene expression, while it down-regulates endocardial and endothelial gene expression. Involved in bone morphogenetic protein (BMP)-mediated induction of cardiac-specific gene expression. Binds to BMP response element (BMPRE) DNA sequences within cardiac activating regions (By similarity). Acts as a transcriptional activator of ANF in cooperation with NKX2-5 (By similarity). Promotes cardiac myocyte enlargement. Required during testicular development. May play a role in sphingolipid signaling by regulating the expression of sphingosine-1-phosphate degrading enzyme, sphingosine-1-phosphate lyase. KEYWORDS: 3D-structure;Activator;Alternative splicing;Atrial septal defect;Cardiomyopathy;Disease variant;DNA-binding;Metal-binding;Methylation;Nucleus;Reference proteome;Repeat;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus " P45436,"PROTEIN NAMES: Cell death protein 3 (Caspase ced-3) [Cleaved into: Cell death protein 3 subunit p17; Cell death protein 3 subunit p15; Cell death protein 3 subunit p13] PROTEIN FAMILY: Peptidase C14A family ORGANISM: Caenorhabditis remanei (Caenorhabditis vulgaris) SIMILARITY: Belongs to the peptidase C14A family. FUNCTION: Acts as a cysteine protease in controlling programmed cell death (apoptosis) by proteolytically activating or inactivating a wide range of substrates. Component of the egl-1, ced-9, ced-4 and ced-3 apoptotic signaling cascade required for the initiation of programmed cell death in cells fated to die during embryonic and postembryonic development. During oogenesis, required for germline apoptosis downstream of ced-9 and ced-4 but independently of egl-1. By cleaving and activating ced-8, promotes phosphatidylserine exposure on the surface of apoptotic cells; phosphatidylserine is a specific marker only present at the surface of apoptotic cells and acts as a specific signal for engulfment. By cleaving and converting dcr-1 into a deoxyribonuclease (DNase), promotes apoptotic chromosomal DNA fragmentation. By cleaving mitochondrial fission protein drp-1, may regulate the removal of mitochondria during apoptosis. During germline apoptosis, cleaves translation initiation factor ifg-1 (isoform p170) promoting cap-independent translation. During male tail morphogenesis, promotes apoptosis of the tail-spike cell downstream of ced-4 but independently of egl-1 and ced-9. By cleaving cnt-1, prevents the activation of the prosurvival akt-1/2 signaling pathway and thus promotes apoptosis. Downstream of ced-4, may play a role in sex-specific cell apoptosis by cleaving sex-determining protein fem-1. May regulate germline apoptosis in response to DNA damage, probably downstream of let-60/ras and mpk-1 pathway. Cleaves ced-9 in vitro. Cleaves csp-2 isoform b resulting in the removal of the propeptide and the generation of csp-2 subunit p31 in vitro. Independently of its apoptotic role has additional functions. Probably by cleaving and thereby activating actin-severing protein gsnl-1, required for the elimination of transient presynaptic components during larval development downstream of egl-1, ced-9 and ced-4 pathway. Together with ain-1, a component of the miRNA-induced-silencing complex (miRISC), regulates temporal cell fate patterning during larval development. Acts in cell fate patterning by cleaving heterochronic protein lin-28, likely promoting its degradation. Also cleaves heterochronic protein lin-14 and exonuclease disl-2 in vitro. Downstream of calreticulin crt-1 and ced-4 and independently of egl-1 and ced-9, plays a role in the initial steps of axonal regrowth following axotomy. Cleaves 14-3-3-like protein ftt-2, tubulin tbb-2 and calreticulin crt-1 in vitro. Plays also a role in resistance to S.typhimurium-mediated infection. KEYWORDS: Apoptosis;Autocatalytic cleavage;Cytoplasm;Hydrolase;Membrane;Mitochondrion;Nucleus;Protease;Reference proteome;Synapse;Thiol protease;Zymogen SUBCELLULAR LOCATION: Nucleus membrane Perikaryon Synapse Mitochondrion Cytoplasm Cytoplasm, perinuclear region Note=Colocalizes with nucleoporin npp-14 to the perinuclear region in germ cells. Becomes diffused in the cytoplasm in apoptotic germ cells. Localizes to axonal mitochondria and synapses of DD motor neurons. Synaptic localization is dependent on axonal mitochondria. " P45442,"PROTEIN NAMES: Protein lag-2 (Lethal protein 461) ORGANISM: Caenorhabditis elegans FUNCTION: Probable ligand for lin-12/Notch and glp-1/Notch receptors and involved in the mediation of Notch signaling. Involved in the lin-12/Notch pathway signaling of cell fate in vulval precursor cells (VPCs) and in the postembryonic mesodermal lineage (M lineage), acting redundantly with dsl-1 and apx-1. Functions in uterine cells to promote basement membrane mobility during tissue remodeling. Required for oocyte growth control, acting redundantly with apx-1, perhaps signaling via the glp-1/Notch pathway. Plays a role in Notch-dependent induction of left-right asymmetry in interneurons and motoneurons. Involved in maintaining the developmentally arrested larval state known as dauer, probably signaling in the glp-1/Notch pathway. Required for normal sleep bout quantity and arousal thresholds during the transition from the last larval stage to adulthood in well-fed animals. KEYWORDS: Cell membrane;Developmental protein;Differentiation;Disulfide bond;EGF-like domain;Glycoprotein;Membrane;Notch signaling pathway;Reference proteome;Repeat;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein " P45448,"PROTEIN NAMES: Nuclear receptor subfamily 5 group A member 2 (Liver receptor homolog 1) (LRH-1) PROTEIN FAMILY: Nuclear hormone receptor family, NR5 subfamily ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the nuclear hormone receptor family. NR5 subfamily. FUNCTION: Nuclear receptor that acts as a key metabolic sensor by regulating the expression of genes involved in bile acid synthesis, cholesterol homeostasis and triglyceride synthesis. Together with the oxysterol receptors NR1H3/LXR-alpha and NR1H2/LXR-beta, acts as an essential transcriptional regulator of lipid metabolism. Plays an anti-inflammatory role during the hepatic acute phase response by acting as a corepressor: inhibits the hepatic acute phase response by preventing dissociation of the N-Cor corepressor complex. Key regulator of cholesterol 7-alpha-hydroxylase gene (CYP7A) expression in liver. May also contribute to the regulation of pancreas-specific genes and play important roles in embryonic development (By similarity). Activates the transcription of CYP2C38. KEYWORDS: 3D-structure;Activator;DNA-binding;Isopeptide bond;Lipid-binding;Metal-binding;Nucleus;Receptor;Reference proteome;Transcription;Transcription regulation;Ubl conjugation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus " P45563,"PROTEIN NAMES: Purine nucleoside phosphorylase 2 (Inosine-guanosine phosphorylase) (Purine nucleoside phosphorylase II) (PNP II) (Xanthosine phosphorylase) PROTEIN FAMILY: PNP/MTAP phosphorylase family ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the PNP/MTAP phosphorylase family. FUNCTION: The purine nucleoside phosphorylases catalyze the phosphorolytic breakdown of the N-glycosidic bond in the beta-(deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate. This protein can degrade all purine nucleosides including xanthosine, inosine and guanosine, but cannot cleave adenosine, deoxyadenosine or hypoxanthine arabinoside. Has a preference for the neutral over the monoanionic form of xanthosine. PATHWAY: Purine metabolism; xanthosine degradation.; PATHWAY: Purine metabolism; purine nucleoside salvage. KEYWORDS: 3D-structure;Glycosyltransferase;Reference proteome;Transferase " P45575,"PROTEIN NAMES: Sulfite reductase, dissimilatory-type subunit beta (Desulfoviridin subunit beta) (Dissimilatory sulfite reductase subunit beta) (dSiR beta) (Hydrogensulfite reductase subunit beta) ORGANISM: Nitratidesulfovibrio vulgaris (strain ATCC 29579 / DSM 644 / CCUG 34227 / NCIMB 8303 / VKM B-1760 / Hildenborough) (Desulfovibrio vulgaris) FUNCTION: Catalyzes the reduction of sulfite to sulfide. This is the terminal oxidation reaction in sulfate respiration, a process catalyzed by the sulfate-reducing bacteria. KEYWORDS: 3D-structure;4Fe-4S;Direct protein sequencing;Iron;Iron-sulfur;Metal-binding;Oxidoreductase;Reference proteome " P45594,"PROTEIN NAMES: Cofilin/actin-depolymerizing factor homolog (Protein D61) (Protein twinstar) PROTEIN FAMILY: Actin-binding proteins ADF family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the actin-binding proteins ADF family. FUNCTION: Exhibits F-actin depolymerizing activity and regulates actin cytoskeleton dynamics. Required for cytokinesis in both mitotic and meiotic cells and for aster migration and separation. Promotes cell motility during ovary development and oogenesis. During larval development, required for the cell rearrangement needed for formation of terminal filaments which are stacks of somatic cells that are important for the initiation of ovarioles. Also required for border cell migration during oogenesis. During border cell migration, required for actin turnover and lamellipodial protrusion. Required for the establishment of planar cell polarity (PCP) where cells adopt a uniform orientation within the plane of an epithelium. During establishment of PCP, required for the redistribution of the PCP core proteins fz and stan/fmi to the proximodistal cell boundary. During pupal development, required for elongation of the retinal cell body and for rhabdomere morphogenesis. Required for mushroom body neuroblast proliferation and axon growth. Plays a role in the positive regulation of protein secretion. Plays a role in the regulation of nuclear localization of actin. Required for the maintenance of epithelial integrity by controlling cell junctions and is also necessary for cell survival and tissue growth through regulation of JNK and yki signaling. KEYWORDS: 3D-structure;Actin-binding;Cell cycle;Cell division;Cytoplasm;Cytoskeleton;Nucleus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton Nucleus matrix MISCELLANEOUS: The name 'twinstar' derives from the characteristic aberrant arrangement of asters seen in mutants." P45894,"PROTEIN NAMES: 5'-AMP-activated protein kinase catalytic subunit alpha-1 (AMPK subunit alpha-1) PROTEIN FAMILY: Protein kinase superfamily, CAMK Ser/Thr protein kinase family, SNF1 subfamily ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily. FUNCTION: Probably does not act as a sensor that couples lifespan to information about energy levels and insulin-like signals. Together with aak-2, involved in the establishment of germline stem cell (GSC) quiescence during dauer development. Plays a role in the maintenance of glycogen stores which are necessary for resistance to hyperosmotic stress. KEYWORDS: Alternative splicing;ATP-binding;Kinase;Nucleotide-binding;Reference proteome;Serine/threonine-protein kinase;Transferase " P45896,PROTEIN NAMES: Dwarfin sma-3 (MAD protein homolog 2) PROTEIN FAMILY: Dwarfin/SMAD family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the dwarfin/SMAD family. FUNCTION: Involved in TGF-beta pathway. Plays a role in male tail tip morphogenesis. KEYWORDS: Cytoplasm;DNA-binding;Metal-binding;Nucleus;Reference proteome;Transcription;Transcription regulation;Zinc SUBCELLULAR LOCATION: Cytoplasm Nucleus Note=In males localizes to the nuclei and cytoplasm during tail tip morphogenesis. In hermaphrodites localizes only to the cytoplasm during tail tip morphogenesis throughout the L4 stage. P45955,"PROTEIN NAMES: Cell division coordinator CpoB PROTEIN FAMILY: CpoB family ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the CpoB family. FUNCTION: Mediates coordination of peptidoglycan synthesis and outer membrane constriction during cell division. Promotes physical and functional coordination of the PBP1B-LpoB and Tol machines, and regulates PBP1B activity in response to Tol energy state. KEYWORDS: 3D-structure;Cell cycle;Cell division;Coiled coil;Periplasm;Reference proteome;Repeat;Signal;TPR repeat SUBCELLULAR LOCATION: Periplasm Note=Targeting to the Sec-translocase for transport across the inner membrane is SecB-dependent. Localizes to the septum concurrent with PBP1B-LpoB and Tol at the onset of constriction. Localization is dependent on divisome assembly and requires ongoing septal peptidoglycan synthesis. " P45975,"PROTEIN NAMES: Histone-lysine N-methyltransferase Su(var)3-9 (Histone H3-K9 methyltransferase) (H3-K9-HMTase) (Lysine N-methyltransferase 1) (Protein suppressor of variegation 3-9) PROTEIN FAMILY: Class V-like SAM-binding methyltransferase superfamily, Histone-lysine methyltransferase family, Suvar3-9 subfamily ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. Suvar3-9 subfamily. FUNCTION: Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3 using monomethylated H3 'Lys-9' as substrate. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting Su(var)205/HP1 to methylated histones. Mainly functions in heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin at pericentric regions. Involved in heterochromatic gene silencing including the modification of position-effect-variegation. KEYWORDS: Centromere;Chromatin regulator;Chromosome;Metal-binding;Methyltransferase;Nucleus;Reference proteome;Repressor;S-adenosyl-L-methionine;Transcription;Transcription regulation;Transferase;Zinc SUBCELLULAR LOCATION: Nucleus. Chromosome, centromere. Chromosome Note=Associates with centromeric constitutive heterochromatin. " P46014,PROTEIN NAMES: Protein phosphatase 2C 70 (AtPP2C70) (Kinase-associated protein phosphatase) (Protein ROOT ATTENUATED GROWTH 1) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Dephosphorylates the Ser/Thr receptor-like kinase RLK5. May function as a signaling component in a pathway involving RLK5. Binds and dephosphorylates CLAVATA1 (CLV1). Functions as a negative regulator of the CLV1 signaling in plant development. Dephosphorylates SERK1 receptor kinase on threonine residues in the A-loop. Dephosphorylation of SERK1 controls SERK1 internalization. Component of a signaling pathway which mediates adaptation to NaCl stress. Is not a component of the SALT OVERLY SENSITIVE (SOS) pathway. KEYWORDS: 3D-structure;Alternative splicing;Cell membrane;Hydrolase;Magnesium;Manganese;Membrane;Metal-binding;Protein phosphatase;Reference proteome;Signal-anchor;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein P46029,"PROTEIN NAMES: Solute carrier family 15 member 2 (Kidney H(+)/peptide cotransporter) (Oligopeptide transporter, kidney isoform) (Peptide transporter 2) PROTEIN FAMILY: Major facilitator superfamily, Proton-dependent oligopeptide transporter (POT/PTR) family ORGANISM: Oryctolagus cuniculus (Rabbit) SIMILARITY: Belongs to the major facilitator superfamily. Proton-dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family. FUNCTION: Proton-coupled amino-acid transporter that transports oligopeptides of 2 to 4 amino acids with a preference for dipeptides. Transports neutral and anionic dipeptides with a proton to peptide stoichiometry of 2:1 or 3:1 (By similarity). In kidney, involved in the absorption of circulating di- and tripeptides from the glomerular filtrate. Can also transport beta-lactam antibiotics, such as the aminocephalosporin cefadroxil, and other antiviral and anticancer drugs. Transports the dipeptide-like aminopeptidase inhibitor bestatin (By similarity). Also able to transport carnosine (By similarity). Involved in innate immunity by promoting the detection of microbial pathogens by NOD-like receptors (NLRs) (By similarity). Mediates transport of bacterial peptidoglycans across the plasma membrane or, in macrophages, the phagosome membrane: catalyzes the transport of certain bacterial peptidoglycans, such as muramyl dipeptide (MDP), the NOD2 ligand (By similarity). KEYWORDS: Cell membrane;Cytoplasmic vesicle;Glycoprotein;Immunity;Innate immunity;Membrane;Peptide transport;Phosphoprotein;Protein transport;Reference proteome;Symport;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Apical cell membrane ; Multi-pass membrane protein Cytoplasmic vesicle, phagosome membrane ; Multi-pass membrane protein Cell membrane ; Multi-pass membrane protein Note=Associated with the cell membrane in resting macrophages and enriched in phagocytic cups and phagosomes after particle internalization. " P46061,"PROTEIN NAMES: Ran GTPase-activating protein 1 (RanGAP1) PROTEIN FAMILY: RNA1 family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the RNA1 family. FUNCTION: GTPase activator for RAN. Converts cytoplasmic GTP-bound RAN to GDP-bound RAN, which is essential for RAN-mediated nuclear import and export. Mediates dissociation of cargo from nuclear export complexes containing XPO1, RAN and RANBP2 after nuclear export (By similarity). Required for postimplantation embryonic development. KEYWORDS: 3D-structure;Acetylation;Centromere;Chromosome;Cytoplasm;Cytoskeleton;GTPase activation;Isopeptide bond;Kinetochore;Leucine-rich repeat;Nucleus;Phosphoprotein;Reference proteome;Repeat;Ubl conjugation SUBCELLULAR LOCATION: Cytoplasm Nucleus, nucleoplasm Nucleus envelope Chromosome, centromere, kinetochore Cytoplasm, cytoskeleton, spindle Note=Cytoplasmic during interphase. Detected at the nuclear envelope during interphase. Shuttles between nucleus and cytoplasm. Targeted to the nuclear pores after sumoylation. During mitosis, associates with mitotic spindles, but is essentially not detected at the spindle poles. Association with kinetochores appears soon after nuclear envelope breakdown and persists until late anaphase. Mitotic location also requires sumoylation (By similarity). " P46092,PROTEIN NAMES: C-C chemokine receptor type 10 (C-C CKR-10) (CC-CKR-10) (CCR-10) (G-protein coupled receptor 2) PROTEIN FAMILY: G-protein coupled receptor 1 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the G-protein coupled receptor 1 family. FUNCTION: Receptor for chemokines SCYA27 and SCYA28. Subsequently transduces a signal by increasing the intracellular calcium ions level and stimulates chemotaxis in a pre-B cell line. KEYWORDS: Cell membrane;Disulfide bond;G-protein coupled receptor;Membrane;Receptor;Reference proteome;Transducer;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. P46198,"PROTEIN NAMES: Translation initiation factor IF-2, mitochondrial (IF-2(Mt)) (IF-2Mt) (IF2(mt)) PROTEIN FAMILY: TRAFAC class translation factor GTPase superfamily, Classic translation factor GTPase family, IF-2 subfamily ORGANISM: Bos taurus (Bovine) SIMILARITY: Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. IF-2 subfamily. FUNCTION: One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex. KEYWORDS: 3D-structure;Direct protein sequencing;GTP-binding;Initiation factor;Mitochondrion;Nucleotide-binding;Phosphoprotein;Protein biosynthesis;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Mitochondrion. " P46489,"PROTEIN NAMES: Malate dehydrogenase [NADP], chloroplastic (NADP-MDH) PROTEIN FAMILY: LDH/MDH superfamily, MDH type 2 family ORGANISM: Flaveria bidentis (Coastal plain yellowtops) (Ethulia bidentis) SIMILARITY: Belongs to the LDH/MDH superfamily. MDH type 2 family. FUNCTION: The chloroplastic, NADP-dependent form is essential for the photosynthesis C4 cycle, which allows plants to circumvent the problem of photorespiration. In C4 plants, NADP-MDH activity acts to convert oxaloacetate to malate in chloroplasts of mesophyll cells for transport to the bundle sheath cells. KEYWORDS: 3D-structure;Chloroplast;Disulfide bond;NADP;Oxidoreductase;Plastid;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast. " P46555,"PROTEIN NAMES: D-glucuronyl C5-epimerase (Heparan sulfate C5-epimerase) (Hsepi) (Heparin/heparan sulfate:glucuronic acid C5-epimerase) (Heparosan-N-sulfate-glucuronate 5-epimerase) PROTEIN FAMILY: D-glucuronyl C5-epimerase family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the D-glucuronyl C5-epimerase family. FUNCTION: Converts D-glucuronic acid residues adjacent to N-sulfate sugar residues to L-iduronic acids (By similarity). Plays a role in the early migration of AQR and PQR neurons, which descend from the Q neuroblasts. PATHWAY: Glycan metabolism; heparan sulfate biosynthesis.; PATHWAY: Glycan metabolism; heparin biosynthesis. KEYWORDS: Basement membrane;Cell membrane;Extracellular matrix;Glycoprotein;Isomerase;Membrane;Reference proteome;Secreted;Signal-anchor;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type II membrane protein Secreted, extracellular space, extracellular matrix, basement membrane. " P46592,"PROTEIN NAMES: Glycolipid 2-alpha-mannosyltransferase 2 (Alpha-1,2-mannosyltransferase 2) PROTEIN FAMILY: Glycosyltransferase 15 family ORGANISM: Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) SIMILARITY: Belongs to the glycosyltransferase 15 family. FUNCTION: Involved in O-glycosylation of cell wall and secreted proteins. Transfers an alpha-D-mannosyl residue from GDP-mannose into lipid-linked oligosaccharide, forming an alpha-(1->2)-D-mannosyl-D-mannose linkage. Mainly responsible for the addition of the third mannose residue in an O-linked mannose pentamer. Can also substitute for MNT1 by adding the second mannose residue. Important for adherence to host surfaces and for virulence. KEYWORDS: Glycosyltransferase;Golgi apparatus;Membrane;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus membrane ; Single-pass type II membrane protein " P46607,"PROTEIN NAMES: Homeobox-leucine zipper protein GLABRA 2 (Protein GLABRA2) (HD-ZIP protein ATHB-10) (Homeobox-leucine zipper protein ATHB-10) PROTEIN FAMILY: HD-ZIP homeobox family, Class IV subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the HD-ZIP homeobox family. Class IV subfamily. FUNCTION: Transcription factor involved in the determination of epidermal cell identity (Probable). Required for correct morphological development and maturation of trichomes. Regulates the frequency of trichome initiation and determines trichome spacing. Acts as a negative factor for root hair development. Required for ectopic repression of root hair development in a subset of epidermal cells. May suppress hair formation in root epidermis by promoting differentiation into hairless epidermal cells. Directly suppresses the bHLH transcription factor genes, RHD6, RSL1, RSL2, LRL1, and LRL2, which have diverse functions in root hair development. Required for normal development of seed coat mucilage. Involved in the control of seed oil accumulation. Acts as a negative regulator of anthocyanin biosynthesis. May directly repress the expression of some component genes from the MYB-bHLH-WD40 (MBW) transcriptional activator complex. The MBW complex activates the transcription of late biosynthesis genes in the flavonoid pathway, leading to the production of anthocyanins (Probable). KEYWORDS: Alternative splicing;Coiled coil;DNA-binding;Homeobox;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: The gain-of-function gl2-1D mutants (T-DNA tagging) exhibit reduced endogenous levels of anthocyanins." P46639,"PROTEIN NAMES: Homeobox protein knotted-1-like 1 (Protein BREVIPEDICELLUS) (Protein KNAT1) PROTEIN FAMILY: TALE/KNOX homeobox family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the TALE/KNOX homeobox family. FUNCTION: May play a role in meristem function, and may be involved in maintaining cells in an undifferentiated, meristematic state, and its expression disappears at the same time the shoot apex undergoes the transition from vegetative to reproductive development. Positive regulator of LATERAL ORGAN BOUNDARIES (LOB). Probably binds to the DNA sequence 5'-TGAC-3'. Able to traffic from the L1 to the L2/L3 layers of the meristem, presumably through plasmodesmata. KEYWORDS: DNA-binding;Homeobox;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus " P46662,"PROTEIN NAMES: Merlin (Moesin-ezrin-radixin-like protein) (Neurofibromin-2) (Schwannomin) ORGANISM: Mus musculus (Mouse) FUNCTION: Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with WWC1 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. May act as a membrane stabilizing protein. May inhibit PI3 kinase by binding to AGAP2 and impairing its stimulating activity. Suppresses cell proliferation and tumorigenesis by inhibiting the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex (By similarity). Plays a role in lens development and is required for complete fiber cell terminal differentiation, maintenance of cell polarity and separation of the lens vesicle from the corneal epithelium. KEYWORDS: 3D-structure;Alternative splicing;Cell membrane;Cell projection;Cytoplasm;Cytoskeleton;Membrane;Nucleus;Phosphoprotein;Reference proteome;Tumor suppressor;Ubl conjugation SUBCELLULAR LOCATION: Cell membrane ; Peripheral membrane protein ; Cytoplasmic side Cell projection Cytoplasm, cytoskeleton Nucleus Note=Colocalizes with MPP1 in non-myelin-forming Schwann cells. Binds with DCAF1 in the nucleus. The intramolecular association of the FERM domain with the C-terminal tail promotes nuclear accumulation. The unphosphorylated form accumulates predominantly in the nucleus while the phosphorylated form is largely confined to the non-nuclear fractions (By similarity). " P46672,"PROTEIN NAMES: tRNA-aminoacylation cofactor ARC1 (Acyl-RNA-complex protein 1) (GU4 nucleic-binding protein 1) (G4p1 protein) (P42) (tRNA-interacting factor ARC1) PROTEIN FAMILY: TRNA-aminoacylation cofactor ARC1 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the tRNA-aminoacylation cofactor ARC1 family. FUNCTION: Binds to tRNA and functions as a cofactor for the methionyl-tRNA synthetase (MetRS) and glutamyl-tRNA synthetase (GluRS). Forms a complex with MetRS and GluRS and increases their affinity for cognate tRNAs due to the presence of a tRNA binding domain in its middle and C-terminal part. Binds specifically G4 quadruplex nucleic acid structures (these are four-stranded right-handed helices, stabilized by guanine base quartets). Also required for cytoplasmic confinement of the synthetases and tRNA. KEYWORDS: 3D-structure;Cytoplasm;Direct protein sequencing;Reference proteome;RNA-binding;tRNA-binding SUBCELLULAR LOCATION: Cytoplasm Note=Largely excluded from the nucleus. MISCELLANEOUS: Present with 57700 molecules/cell in log phase SD medium." P46678,PROTEIN NAMES: Transcription factor TFIIIB component B'' (TFIIIB90) PROTEIN FAMILY: TFC5 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the TFC5 family. FUNCTION: General activator of RNA polymerase III transcription. KEYWORDS: 3D-structure;Activator;Direct protein sequencing;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus. P46683,PROTEIN NAMES: Ankyrin repeat-containing protein YAR1 ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Required for normal rate of cell proliferation. KEYWORDS: 3D-structure;ANK repeat;Phosphoprotein;Reference proteome;Repeat MISCELLANEOUS: Present with 13100 molecules/cell in log phase SD medium. P46869,"PROTEIN NAMES: Kinesin-like protein FLA10 (Protein KHP1) PROTEIN FAMILY: TRAFAC class myosin-kinesin ATPase superfamily, Kinesin family, Kinesin II subfamily ORGANISM: Chlamydomonas reinhardtii (Chlamydomonas smithii) SIMILARITY: Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. Kinesin II subfamily. FUNCTION: Probably involved in flagellar assembly and maintenance. May play a role in flagellar synthesis. KEYWORDS: ATP-binding;Coiled coil;Cytoplasm;Cytoskeleton;Microtubule;Motor protein;Nucleotide-binding SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton " P46873,"PROTEIN NAMES: Osmotic avoidance abnormal protein 3 (Kinesin-like protein osm-3) PROTEIN FAMILY: TRAFAC class myosin-kinesin ATPase superfamily, Kinesin family, Kinesin II subfamily ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. Kinesin II subfamily. FUNCTION: Kinesin motor protein which is required for the anterograde intraflagellar transport (IFT) along the middle segment of the sensory neuron cilia together with the kinesin II motor complex (composed of klp-11, klp-20 and kap-1) and on its own, is required for IFT along the distal segment. In addition, regulates the length of cilia. May have a role during neurogenesis and axonal transport. KEYWORDS: 3D-structure;Alternative splicing;ATP-binding;Cell projection;Coiled coil;Cytoplasm;Cytoskeleton;Microtubule;Motor protein;Nucleotide-binding;Reference proteome SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton Cell projection, cilium Cytoplasm, cytoskeleton, cilium axoneme Cytoplasm, cytoskeleton, cilium basal body Note=Localizes along the full cilium length. " P46925,"PROTEIN NAMES: Plasmepsin II (PLM II) (Aspartic hemoglobinase II) (PfAPD) (PfPM1) (Plasmepsin 2) PROTEIN FAMILY: Peptidase A1 family ORGANISM: Plasmodium falciparum (isolate HB3) SIMILARITY: Belongs to the peptidase A1 family. FUNCTION: During the asexual blood stage, participates in initial cleavage of native host hemoglobin (Hb) resulting in Hb denaturation. May cleave preferentially denatured hemoglobin that has been cleaved by PMI. Digestion of host Hb is an essential step which provides the parasite with amino acids for protein synthesis, and regulates osmolarity (Probable). KEYWORDS: 3D-structure;Aspartyl protease;Direct protein sequencing;Disulfide bond;Hydrolase;Membrane;Protease;Reference proteome;Signal-anchor;Transmembrane;Transmembrane helix;Vacuole;Zymogen SUBCELLULAR LOCATION: Membrane ; Single-pass type II membrane protein Vacuole lumen Vacuole membrane Note=At the beginning of the asexual blood stage, the transmembrane zymogen is transported to the cytostome, an endocytic structure spanning the parasite cell membrane and the parasitophorous vacuole membrane where host proteins such as hemoglobin are endocytosed. Following endocytosis, localizes to the cytostome vacuole membrane to be then delivered to the digestive (or food) vacuole where it is cleaved into the soluble and active enzyme. In trophozoites, localizes to the digestive vacuole, an acidic vacuole where host hemoglobin is digested. " P46933,"PROTEIN NAMES: Amyloid beta precursor protein binding family B member 1 (Amyloid-beta A4 precursor protein-binding family B member 1) (Protein Fe65) ORGANISM: Rattus norvegicus (Rat) FUNCTION: Transcription coregulator that can have both coactivator and corepressor functions. Adapter protein that forms a transcriptionally active complex with the gamma-secretase-derived amyloid precursor protein (APP) intracellular domain. Plays a central role in the response to DNA damage by translocating to the nucleus and inducing apoptosis. May act by specifically recognizing and binding histone H2AX phosphorylated on 'Tyr-142' (H2AXY142ph) at double-strand breaks (DSBs), recruiting other pro-apoptosis factors such as MAPK8/JNK1. Required for histone H4 acetylation at double-strand breaks (DSBs) (By similarity). Its ability to specifically bind modified histones and chromatin modifying enzymes such as KAT5/TIP60, probably explains its transcription activation activity. Functions in association with TSHZ3, SET and HDAC factors as a transcriptional repressor, that inhibits the expression of CASP4. Associates with chromatin in a region surrounding the CASP4 transcriptional start site(s) (By similarity). Involved in hippocampal neurite branching and neuromuscular junction formation, as a result plays a role in spatial memory functioning. Plays a role in the maintenance of lens transparency. May play a role in muscle cell strength (By similarity). Acts as a molecular adapter that functions in neurite outgrowth by activating the RAC1-ARF6 axis upon insulin treatment (By similarity). KEYWORDS: Acetylation;Activator;Alternative splicing;Apoptosis;Cell membrane;Cell projection;Chromatin regulator;Cytoplasm;DNA damage;Membrane;Nucleus;Phosphoprotein;Reference proteome;Repeat;Repressor;Transcription;Transcription regulation;Ubl conjugation SUBCELLULAR LOCATION: Cell membrane Cytoplasm Nucleus Cell projection, growth cone Nucleus speckle Note=Colocalizes with TSHZ3 in axonal growth cone. Colocalizes with TSHZ3 in the nucleus. In normal conditions, it mainly localizes to the cytoplasm, while a small fraction is tethered to the cell membrane via its interaction with APP. Following exposure to DNA damaging agents, it is released from cell membrane and translocates to the nucleus. Nuclear translocation is under the regulation of APP. Colocalizes with NEK6 at the nuclear speckles (By similarity). Phosphorylation at Ser-610 by SGK1 promotes its localization to the nucleus. " P46946,"PROTEIN NAMES: DNA endonuclease SAE2 (Completion of meiotic recombination protein 1) (Sporulation in the absence of SPO11 protein 2) PROTEIN FAMILY: COM1/SAE2/CtIP family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the COM1/SAE2/CtIP family. FUNCTION: Endonuclease that cooperates with the MRX complex in processing meiotic and mitotic double-strand breaks by allowing the endonucleolytic removal of SPO11 from the break sites and ensuring both resection and intrachromosomal association of the broken ends. Required for proper recovery from checkpoint-mediated cell cycle arrest after DNA damage. MRX complex and SAE2 remove a small oligonucleotide(s) from the DNA ends to form an early intermediate which is rapidly processed by EXO1 and/or SGS1 to generate extensive tracts of single-stranded DNA that serve as substrate for RAD51. Plays a transitional role in the dissociation of MRE11 from, and the recruitment of RAD52 to, repair foci. Ensures that both ends of a DSB participate in a recombination event and impairs the formation of palindromic structures in the genome. With TEL1, promotes microhomology-mediated end joining (MMEJ) but inhibits non-homologous end joining (NHEJ), likely by regulating MRE11-dependent ssDNA accumulation at DNA break. SAE2 and MRX are particularly important for removal of hairpins, bulky adducts and other irregular end structures. Facilitates telomere length reequilibration and subsequent checkpoint switch off. Involved in homing efficiency of VMA1 intein VDE and in repair of transposon excision sites. KEYWORDS: Cytoplasm;DNA damage;DNA repair;DNA-binding;Endonuclease;Hydrolase;Meiosis;Nuclease;Nucleus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Nucleus Note=Accumulates in foci at the precise time when MRE11 foci disassemble and RAD52 foci assemble. Remains associated with DSBs along with MRE11 in nuclease-deficient cells. MISCELLANEOUS: Present with 1030 molecules/cell in log phase SD medium." P46962,"PROTEIN NAMES: CTD kinase subunit beta (CTDK-I subunit beta) (CTD kinase 38 kDa subunit) (CTD kinase subunit 2) PROTEIN FAMILY: Cyclin family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the cyclin family. FUNCTION: Cyclin subunit of the CTDK-I complex, which hyperphosphorylates the C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit. CTDK-I phosphorylates 'Ser-5' if the CTD substrate is not phosphorylated at 'Ser-5', but will phosphorylate 'Ser-2' of a CTD substrate if 'Ser-5' is already phosphorylated. CTDK-I is also more reactive toward substrates that are prephosphorylated at 'Ser-2' or 'Ser-5' compared with an unphosphorylated CTD substrate, therefore efficiently creating doubly phosphorylated CTD repeats. Involved in RNA polymerase II transcriptional elongation, and as part of the CTDK-I complex, pre-mRNA 3'-end processing and SET2 mediated H3K36 methylation. Together with CTK3, required for CTK1 CTD kinase activation. Required for DNA damage induced transcription. Involved in the adaptation to alternative carbon sources, including galactose, glycerol and ethanol, but not raffinose. Required for the integrity of the rDNA locus. KEYWORDS: 3D-structure;Cyclin;Direct protein sequencing;DNA damage;mRNA processing;Nucleus;Phosphoprotein;Reference proteome;Transcription;Ubl conjugation SUBCELLULAR LOCATION: Nucleus, nucleolus MISCELLANEOUS: Present with 1590 molecules/cell in log phase SD medium." P47013,PROTEIN NAMES: Dihydrosphingosine 1-phosphate phosphatase LCB3 (Long-chain base protein 3) (Sphingolipid resistance protein 2) PROTEIN FAMILY: Type 2 lipid phosphate phosphatase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the type 2 lipid phosphate phosphatase family. FUNCTION: Dihydrosphingosine 1-phosphate phosphatase required for efficient ceramide synthesis from exogenous sphingoid bases. Involved in endocytosis and calcium-mediated signaling. KEYWORDS: Endoplasmic reticulum;Glycoprotein;Hydrolase;Lipid metabolism;Membrane;Phosphoprotein;Reference proteome;Sphingolipid metabolism;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein P47017,"PROTEIN NAMES: Sm-like protein LSm1 (SPB8 protein) PROTEIN FAMILY: SnRNP Sm proteins family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the snRNP Sm proteins family. FUNCTION: Component of the cytoplasmic LSM1-LSM7 complex which is involved in mRNA degradation by activating the decapping step. The LSM1-LSM7 complex binds RNA with a preference for poly-U ends. KEYWORDS: 3D-structure;Cytoplasm;mRNA processing;Nucleus;Reference proteome;Ribonucleoprotein;RNA-binding SUBCELLULAR LOCATION: Nucleus Cytoplasm Cytoplasm, P-body MISCELLANEOUS: Present with 3490 molecules/cell in log phase SD medium." P47026,"PROTEIN NAMES: GPI-anchored wall transfer protein 1 PROTEIN FAMILY: PIGW family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the PIGW family. FUNCTION: Probable acetyltransferase, which acetylates the inositol ring of phosphatidylinositol during biosynthesis of GPI-anchor. Acetylation during GPI-anchor biosynthesis is not essential for the subsequent mannosylation and is usually removed soon after the attachment of GPIs to proteins. PATHWAY: Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis. KEYWORDS: Acyltransferase;Endoplasmic reticulum;GPI-anchor biosynthesis;Membrane;Reference proteome;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein MISCELLANEOUS: Target of the antifungal compound 1-[4-butylbenzyl]isoquinoline that inhibits cell wall localization of GPI-anchored mannoproteins." P47027,PROTEIN NAMES: DNA replication regulator DPB11 ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Has a role in the initiation of DNA replication. Required at S-phase checkpoint. Required for the association of PSF1 with origins. Also required for the proper activation of RAD53 in response to DNA damage and replication blocks. Multicopy suppressor of DPB2 mutation. Overexpression restores the growth defect conferred by POL2 mutation. KEYWORDS: Cell cycle;DNA replication;Nucleus;Reference proteome;Repeat SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: Present with 540 molecules/cell in log phase SD medium. P47039,PROTEIN NAMES: Probable kynurenine--oxoglutarate transaminase BNA3 (Biosynthesis of nicotinic acid protein 3) (Kynurenine aminotransferase) PROTEIN FAMILY: Class-I pyridoxal-phosphate-dependent aminotransferase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. FUNCTION: Catalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid (KA). PATHWAY: Amino-acid degradation; L-kynurenine degradation; kynurenate from L-kynurenine: step 1/2. KEYWORDS: 3D-structure;Aminotransferase;Cytoplasm;Mitochondrion;Pyridoxal phosphate;Reference proteome;Transferase SUBCELLULAR LOCATION: Cytoplasm Mitochondrion MISCELLANEOUS: Present with 1600 molecules/cell in log phase SD medium. P47041,"PROTEIN NAMES: Target of rapamycin complex 2 subunit BIT61 (TORC2 subunit BIT61) (61 kDa binding partner of TOR2 protein) PROTEIN FAMILY: BIT61 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the BIT61 family. FUNCTION: Component of TORC2, which regulates cell cycle-dependent polarization of the actin-cytoskeleton and cell wall integrity. TORC2 controls polarity of the actin cytoskeleton, which is required for orienting the secretory pathway toward discrete growth sites, via the RHO1/PKC1/MAPK cell integrity pathway. KEYWORDS: Cell membrane;Membrane;Phosphoprotein;Reference proteome;Vacuole SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein; Cytoplasmic side. Vacuole membrane ; Peripheral membrane protein ; Cytoplasmic side MISCELLANEOUS: Present with 1960 molecules/cell in log phase SD medium." P47045,"PROTEIN NAMES: Mitochondrial import inner membrane translocase subunit TIM54 PROTEIN FAMILY: TIM54 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the TIM54 family. FUNCTION: Essential component of the TIM22 complex, a complex that mediates the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane. The TIM22 complex forms a twin-pore translocase that uses the membrane potential as external driving force. Its precise function within the TIM22 complex is unclear. KEYWORDS: 3D-structure;Membrane;Mitochondrion;Mitochondrion inner membrane;Protein transport;Reference proteome;Translocation;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Mitochondrion inner membrane ; Single-pass membrane protein Note=Import into inner membrane protein requires TOM70 function. " P47058,"PROTEIN NAMES: tRNA-specific adenosine deaminase subunit TAD2 (tRNA-specific adenosine-34 deaminase subunit TAD2) PROTEIN FAMILY: Cytidine and deoxycytidylate deaminase family, ADAT2 subfamily ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the cytidine and deoxycytidylate deaminase family. ADAT2 subfamily. FUNCTION: Deaminates adenosine-34 to inosine in many tRNAs. KEYWORDS: 3D-structure;Hydrolase;Metal-binding;Reference proteome;tRNA processing;Zinc MISCELLANEOUS: Present with 830 molecules/cell in log phase SD medium." P47074,PROTEIN NAMES: Spindle assembly checkpoint component MAD3 (Mitotic MAD3 protein) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: To yeast protein kinase BUB1 in its non-catalytic N-terminal domain. FUNCTION: Component of the spindle assembly checkpoint which is a feedback control that prevents cells with incompletely assembled spindles from leaving mitosis. Component of the mitotic checkpoint complex (MCC) which inhibits the ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) by preventing its activation by CDC20. KEYWORDS: 3D-structure;Cell cycle;Cell division;Mitosis;Nucleus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Nucleus. MISCELLANEOUS: Present with 3170 molecules/cell in log phase SD medium. P47076,"PROTEIN NAMES: DNA-directed RNA polymerase III subunit RPC9 (RNA polymerase III subunit C9) (RNA polymerase III subunit C17) PROTEIN FAMILY: Eukaryotic RPC9 RNA polymerase subunit family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the eukaryotic RPC9 RNA polymerase subunit family. FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. The RPC25/RPC8-RPC17/RPC9 subcomplex may bind Pol III transcripts emerging from the adjacent exit pore during elongation. KEYWORDS: 3D-structure;DNA-directed RNA polymerase;Nucleus;Phosphoprotein;Reference proteome;Transcription SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: Present with 2930 molecules/cell in log phase SD medium." P47082,"PROTEIN NAMES: Vacuolar amino acid transporter 1 PROTEIN FAMILY: Amino acid/polyamine transporter 2 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the amino acid/polyamine transporter 2 family. FUNCTION: Amino acid/proton antiporter that moves various amino acids into the vacuole, with a preference for large neutral ones and histidine. KEYWORDS: Amino-acid transport;Membrane;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix;Transport;Vacuole SUBCELLULAR LOCATION: Vacuole membrane ; Multi-pass membrane protein MISCELLANEOUS: Present with 1820 molecules/cell in log phase SD medium." P47103,PROTEIN NAMES: Peptidyl-prolyl cis-trans isomerase CYP7 (PPIase CYP7) (Rotamase CYP7) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Plays a major role in negative regulation of the heat shock transcription factor (HSF). KEYWORDS: 3D-structure;Isomerase;Reference proteome;Repeat;Rotamase;TPR repeat MISCELLANEOUS: Present with 3230 molecules/cell in log phase SD medium. P47110,PROTEIN NAMES: DNA polymerase delta subunit 3 ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: DNA polymerase delta (DNA polymerase III) participates in chromosomal DNA replication. It is required during synthesis of the leading and lagging DNA strands at the replication fork and binds at/or near replication origins and moves along DNA with the replication fork. It has 3'-5' proofreading exonuclease activity that correct errors arising during DNA replication. It is also involved in DNA synthesis during DNA repair. KEYWORDS: 3D-structure;DNA replication;DNA-directed DNA polymerase;Nucleotidyltransferase;Nucleus;Phosphoprotein;Reference proteome;Transferase SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: Present with 2410 molecules/cell in log phase SD medium. P47114,"PROTEIN NAMES: Low affinity K(+) transporter 1 PROTEIN FAMILY: KCH1 low affinity K(+) transporter family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the KCH1 low affinity K(+) transporter family. FUNCTION: Low affinity potassium transporter that, with PRM6/KCH2, participates in high-affinity Ca(2+) influx system (HACS) activation during the response to mating pheromone. Directly promotes K(+) influx and HACS may electrochemically respond to this K(+) influx. KCH1 and KCH2 act at the apex of the calcium signaling pathway that is used for survival during prolonged exposures to mating pheromones. KEYWORDS: Cell membrane;Ion transport;Membrane;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix;Transport;Vacuole SUBCELLULAR LOCATION: Vacuole membrane ; Multi-pass membrane protein Cell membrane ; Multi-pass membrane protein MISCELLANEOUS: Present with 538 molecules/cell in log phase SD medium." P47122,PROTEIN NAMES: GPN-loop GTPase 1 (Essential PCL1-interacting ATPase 1) (GPN-loop GTPase NPA3) (Nucleolar preribosomal-associated protein 3) PROTEIN FAMILY: GPN-loop GTPase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the GPN-loop GTPase family. FUNCTION: Small GTPase required for proper nuclear import of RNA polymerase II (RNAPII). May act at an RNAP assembly step prior to nuclear import. Promotes sister chromatid separation during anaphase. KEYWORDS: 3D-structure;Cytoplasm;GTP-binding;Hydrolase;Isopeptide bond;Nucleotide-binding;Phosphoprotein;Reference proteome;Ubl conjugation SUBCELLULAR LOCATION: Cytoplasm MISCELLANEOUS: Present with 15200 molecules/cell in log phase SD medium. P47124,"PROTEIN NAMES: Putative glycosyltransferase HOC1 (M-Pol II subunit Hoc1p) (Mannan polymerase II complex HOC1 subunit) PROTEIN FAMILY: Glycosyltransferase 32 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the glycosyltransferase 32 family. FUNCTION: The M-Pol II complex possesses alpha-1,6-mannosyltransferase activity and is probably involved in the elongation of the mannan backbone of N-linked glycans on cell wall and periplasmic proteins. KEYWORDS: Direct protein sequencing;Glycoprotein;Glycosyltransferase;Golgi apparatus;Membrane;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus, cis-Golgi network membrane ; Single-pass type II membrane protein MISCELLANEOUS: Present with 7160 molecules/cell in log phase SD medium." P47130,PROTEIN NAMES: Cop9 signalosome complex subunit 12 PROTEIN FAMILY: CSN12 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the CSN12 family. FUNCTION: Component of the COP9 signalosome (CSN) complex that acts as an regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunit of SCF-type E3 ubiquitin-protein ligase complexes. The CSN complex is involved in the regulation of the mating pheromone response. CSN12 forms a complex with THP3 that is recruited to transcribed genes and required for transcription elongation. KEYWORDS: 3D-structure;Cytoplasm;Nucleus;Reference proteome;Signalosome;Transcription SUBCELLULAR LOCATION: Cytoplasm Nucleus MISCELLANEOUS: Present with 1510 molecules/cell in log phase SD medium. P47142,"PROTEIN NAMES: Vacuolar protein-sorting-associated protein 25 (ESCRT-II complex subunit VPS25) PROTEIN FAMILY: VPS25 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the VPS25 family. FUNCTION: Component of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. KEYWORDS: 3D-structure;Cytoplasm;Endosome;Membrane;Protein transport;Reference proteome;Transport SUBCELLULAR LOCATION: Cytoplasm Endosome membrane ; Peripheral membrane protein " P47143,PROTEIN NAMES: Adenosine kinase PROTEIN FAMILY: Carbohydrate kinase PfkB family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the carbohydrate kinase PfkB family. FUNCTION: ATP dependent phosphorylation of adenosine and other related nucleoside analogs to monophosphate derivatives. ADO1 does not play a major role in adenine utilization in yeast. Its physiological role could primarily be to recycle adenosine produced by the methyl cycle. PATHWAY: Purine metabolism; AMP biosynthesis via salvage pathway; AMP from adenosine: step 1/1. KEYWORDS: ATP-binding;Kinase;Nucleotide-binding;Purine salvage;Reference proteome;Transferase MISCELLANEOUS: Present with 22200 molecules/cell in log phase SD medium. P47158,"PROTEIN NAMES: Putative transferase CAF17, mitochondrial (57 kDa iron-sulfur cluster assembly factor for biotin synthase- and aconitase-like mitochondrial proteins) (CCR4-associated factor 17) PROTEIN FAMILY: GcvT family, CAF17 subfamily ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the GcvT family. CAF17 subfamily. FUNCTION: Required for lysine and glutamate prototrophy and mitochondrial genome maintenance. Has a role in the maturation of mitochondrial aconitase-type and radical-SAM Fe/S proteins biotin synthase and lipoic acid synthase. KEYWORDS: Mitochondrion;Reference proteome;Transferase;Transit peptide SUBCELLULAR LOCATION: Mitochondrion matrix MISCELLANEOUS: Present with 2960 molecules/cell in log phase SD medium." P47735,"PROTEIN NAMES: Receptor-like protein kinase 5 (Protein HAESA) PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. FUNCTION: Receptor with a dual specificity kinase activity acting on both serine/threonine- and tyrosine-containing substrates that controls floral organ abscission. May interact with the 'INFLORESCENCE DEFICIENT IN ABSCISSION' (IDA) ligands family. KEYWORDS: 3D-structure;ATP-binding;Cell membrane;Glycoprotein;Kinase;Leucine-rich repeat;Membrane;Nucleotide-binding;Phosphoprotein;Reference proteome;Repeat;Serine/threonine-protein kinase;Signal;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein MISCELLANEOUS: The name HAESA derives from a Latin word meaning 'to adhere to'." P47787,"PROTEIN NAMES: Thromboxane-A synthase (TXA synthase) (TXS) (Cytochrome P450 5A1) (Hydroperoxy icosatetraenoate dehydratase) PROTEIN FAMILY: Cytochrome P450 family ORGANISM: Sus scrofa (Pig) SIMILARITY: Belongs to the cytochrome P450 family. FUNCTION: Catalyzes the conversion of prostaglandin H2 (PGH2) to thromboxane A2 (TXA2), a potent inducer of blood vessel constriction and platelet aggregation. Cleaves also PGH2 to 12-hydroxy-heptadecatrienoicacid (12-HHT) and malondialdehyde, which is known to act as a mediator of DNA damage. 12-HHT and malondialdehyde are formed stoichiometrically in the same amounts as TXA2. Additionally, displays dehydratase activity, toward (15S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoate (15(S)-HPETE) producing 15-KETE and 15-HETE (By similarity). KEYWORDS: Endoplasmic reticulum;Fatty acid biosynthesis;Fatty acid metabolism;Heme;Iron;Isomerase;Lipid biosynthesis;Lipid metabolism;Lyase;Membrane;Metal-binding;Monooxygenase;Oxidoreductase;Prostaglandin biosynthesis;Prostaglandin metabolism;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein " P47818,PROTEIN NAMES: Protein CCC1 (Cross-complementer of CSG1 protein 1) PROTEIN FAMILY: CCC1 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the CCC1 family. FUNCTION: Has a role in both calcium and manganese homeostasis. Involved in the transfer of iron and Mn(2+) from the cytosol to the vacuole for storage of these metals. KEYWORDS: Golgi apparatus;Membrane;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix;Transport;Vacuole SUBCELLULAR LOCATION: Golgi apparatus membrane Vacuole membrane ; Multi-pass membrane protein MISCELLANEOUS: Present with 2840 molecules/cell in log phase SD medium. P47979,PROTEIN NAMES: Zinc finger protein zfs1 (Multicopy suppressor of overexpressed cyr1 protein 4) ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) FUNCTION: Binds to specific AU-rich elements (ARE) in the 3'-untranslated region of target mRNAs and promotes their degradation. Binds to ARE present in the arz1 mRNA and stimulates the rate of arz1 mRNA decay. Required for coordination of septum formation with exit from mitosis. Involved in the mating response pathway. Induces sexual development and ascus formation. KEYWORDS: Cell cycle;Cell division;Cytoplasm;Metal-binding;Nucleus;Reference proteome;Repeat;RNA-binding;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cytoplasm Nucleus P48000,PROTEIN NAMES: Homeobox protein knotted-1-like 3 (Protein KNAT3) PROTEIN FAMILY: TALE/KNOX homeobox family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the TALE/KNOX homeobox family. KEYWORDS: Alternative splicing;DNA-binding;Homeobox;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus P48008,"PROTEIN NAMES: Septin homolog spn3 PROTEIN FAMILY: TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily, Septin GTPase family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family. FUNCTION: Plays a role in the cell cycle. Involved in a late stage of septum formation leading to the separation of the daughter cells. KEYWORDS: Cell cycle;Cell division;Coiled coil;Cytoplasm;GTP-binding;Mitosis;Nucleotide-binding;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cytoplasm, cell cortex Note=Localizes to the medial ring at the cell cortex of dividing cells. " P48010,"PROTEIN NAMES: Septin homolog spn5 (Mei4-dependent protein 9) (Meiotic expression up-regulated protein 28) PROTEIN FAMILY: TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily, Septin GTPase family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family. FUNCTION: Septin-like protein involved in the correct orientation of forespore membrane extension during sporulation. KEYWORDS: Coiled coil;GTP-binding;Meiosis;Membrane;Nucleotide-binding;Nucleus;Reference proteome;Sporulation SUBCELLULAR LOCATION: Nucleus. Forespore membrane; Peripheral membrane protein. Note=The sporulation-specific septin complex associates to the forespore membrane and forms partial or complete ring-like structures that curl around each haploid nucleus. " P48236,"PROTEIN NAMES: Glycerophosphocholine acyltransferase 1 (GPCAT) PROTEIN FAMILY: GPC1 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the GPC1 family. FUNCTION: Glycerophosphocholine acyltransferase (GPCAT) that utilizes acyl-CoA to acylate glycero-3-phosphocholine (GPC), forming lysophosphatidylcholine (LPC). Shows broad acyl specificities with a preference for 16:0-CoA, polyunsaturated acyl-CoA, and the hydroxylated ricinoleoyl-CoA. Catalyzes also the acylation of glycero-3-phosphoethanolamine (GPE) with acyl-CoA. In addition to acyl-CoA, GPCAT efficiently utilizes LPC and lysophosphatidylethanolamine (LPE) as acyl donors in the acylation of GPC. Contributes to the maintenance of phosphatidylcholine (PC) homeostasis and might also have specific functions in acyl editing of PC, such as transferring acyl groups modified at the sn-2 position of PC to the sn-1. Involved in postsynthetic PC remodeling that produces more saturated PC species. KEYWORDS: Acyltransferase;Lipid biosynthesis;Lipid metabolism;Membrane;Phospholipid biosynthesis;Phospholipid metabolism;Phosphoprotein;Reference proteome;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Multi-pass membrane protein " P48269,"PROTEIN NAMES: Cytochrome c biogenesis protein CcsA PROTEIN FAMILY: CcmF/CycK/Ccl1/NrfE/CcsA family ORGANISM: Chlamydomonas reinhardtii (Chlamydomonas smithii) SIMILARITY: Belongs to the CcmF/CycK/Ccl1/NrfE/CcsA family. FUNCTION: Required during biogenesis of c-type cytochromes (cytochrome c6 and cytochrome f) at the step of heme attachment. KEYWORDS: Chloroplast;Cytochrome c-type biogenesis;Membrane;Plastid;Reference proteome;Thylakoid;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane ; Multi-pass membrane protein MISCELLANEOUS: Met-1 is the initiator; however when Met-1 is mutated a low amount of protein may be produced, probably from Met-20." P48307,"PROTEIN NAMES: Tissue factor pathway inhibitor 2 (TFPI-2) (Placental protein 5) (PP5) ORGANISM: Homo sapiens (Human) FUNCTION: May play a role in the regulation of plasmin-mediated matrix remodeling. Inhibits trypsin, plasmin, factor VIIa/tissue factor and weakly factor Xa. Has no effect on thrombin. KEYWORDS: 3D-structure;Alternative splicing;Blood coagulation;Direct protein sequencing;Disulfide bond;Glycoprotein;Hemostasis;Protease inhibitor;Reference proteome;Repeat;Secreted;Serine protease inhibitor;Signal SUBCELLULAR LOCATION: Secreted. " P48360,"PROTEIN NAMES: Probable NADPH:adrenodoxin oxidoreductase, mitochondrial (AR) (Adrenodoxin reductase) (Ferredoxin--NADP(+) reductase) (FDXR) (Ferredoxin reductase) PROTEIN FAMILY: Ferredoxin--NADP reductase type 1 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the ferredoxin--NADP reductase type 1 family. FUNCTION: Adrenodoxin reductase transfers electrons from NADPH to adrenodoxin, which is involved in heme A biosynthesis and in iron-sulfur cluster assembly. Involved in the electron transfer to heme A synthase COX15, a heme protein that catalyzes the conversion of heme O to heme A. Required for the de novo synthesis of Fe-S clusters on iron sulfur cluster assembly protein ISU1. Involved in electron delivery for Fe-S cluster synthesis. Essential for coenzyme Q biosynthesis. May be involved in the electron transfer required for the hydroxylation reaction performed by COQ6. May play a role in cellular and mitochondrial iron homeostasis. KEYWORDS: Electron transport;FAD;Flavoprotein;Membrane;Mitochondrion;Mitochondrion inner membrane;NADP;Oxidoreductase;Reference proteome;Transit peptide;Transport SUBCELLULAR LOCATION: Mitochondrion inner membrane ; Peripheral membrane protein MISCELLANEOUS: Present with 1600 molecules/cell in log phase SD medium." P48412,PROTEIN NAMES: Nonsense-mediated mRNA decay protein 3 (Up-frameshift suppressor 3) PROTEIN FAMILY: RENT3 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the RENT3 family. FUNCTION: Involved in nonsense-mediated decay of mRNAs containing premature stop codons. KEYWORDS: Nonsense-mediated mRNA decay;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: Present with 1250 molecules/cell in log phase SD medium. P48421,PROTEIN NAMES: Cytochrome P450 83A1 (CYPLXXXIII) (Protein REDUCED EPIDERMAL FLUORESCENCE 2) PROTEIN FAMILY: Cytochrome P450 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the cytochrome P450 family. FUNCTION: Involved in the metabolism of aliphatic and aromatic oximes. Involved in the biosynthesis of both short-chain and long-chain aliphatic glucosinolates. KEYWORDS: Endoplasmic reticulum;Heme;Iron;Membrane;Metal-binding;Monooxygenase;Oxidoreductase;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Single-pass membrane protein P48437,"PROTEIN NAMES: Prospero homeobox protein 1 (Homeobox prospero-like protein PROX1) (PROX-1) PROTEIN FAMILY: Prospero homeodomain family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the Prospero homeodomain family. FUNCTION: Transcription factor involved in developmental processes such as cell fate determination, gene transcriptional regulation and progenitor cell regulation in a number of organs. Plays a critical role in embryonic development and functions as a key regulatory protein in neurogenesis and the development of the heart, eye lens, liver, pancreas and the lymphatic system. Involved in the regulation of the circadian rhythm. Represses: transcription of the retinoid-related orphan receptor RORG, transcriptional activator activity of RORA and RORG and the expression of RORA/G-target genes including core clock components: BMAL1, NPAS2 and CRY1 and metabolic genes: AVPR1A and ELOVL3. KEYWORDS: Biological rhythms;Developmental protein;DNA-binding;Homeobox;Isopeptide bond;Nucleus;Phosphoprotein;Reference proteome;Repressor;Transcription;Transcription regulation;Ubl conjugation SUBCELLULAR LOCATION: Nucleus Note=RORG promotes its nuclear localization. " P48439,"PROTEIN NAMES: Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 3 (Oligosaccharyl transferase 34 kDa subunit) (Oligosaccharyl transferase subunit OST3) (Oligosaccharyl transferase subunit gamma) PROTEIN FAMILY: OST3/OST6 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the OST3/OST6 family. FUNCTION: Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity. PATHWAY: Protein modification; protein glycosylation. KEYWORDS: 3D-structure;Direct protein sequencing;Disulfide bond;Endoplasmic reticulum;Membrane;Reference proteome;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein MISCELLANEOUS: Present with 4030 molecules/cell in log phase SD medium." P48450,"PROTEIN NAMES: Lanosterol synthase (2,3-epoxysqualene--lanosterol cyclase) (Oxidosqualene--lanosterol cyclase) (OSC) PROTEIN FAMILY: Terpene cyclase/mutase family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the terpene cyclase/mutase family. FUNCTION: Key enzyme in the cholesterol biosynthesis pathway. Catalyzes the cyclization of (S)-2,3 oxidosqualene to lanosterol, a reaction that forms the sterol nucleus. Through the production of lanosterol may regulate lens protein aggregation and increase transparency (By similarity). PATHWAY: Terpene metabolism; lanosterol biosynthesis; lanosterol from farnesyl diphosphate: step 3/3. KEYWORDS: Acetylation;Direct protein sequencing;Endoplasmic reticulum;Isomerase;Lipid biosynthesis;Lipid metabolism;Membrane;Reference proteome;Repeat;Steroid biosynthesis SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Peripheral membrane protein " P48561,"PROTEIN NAMES: Poly(A) RNA polymerase protein 1 (Topoisomerase 1-related protein TRF5) PROTEIN FAMILY: DNA polymerase type-B-like family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the DNA polymerase type-B-like family. FUNCTION: Catalytic subunit of the TRAMP5 complex which has a poly(A) RNA polymerase activity and is involved in a post-transcriptional quality control mechanism limiting inappropriate expression of genetic information. Polyadenylation is required for the degradative activity of the exosome on several of its nuclear RNA substrates like cryptic transcripts generated by RNA polymerase II and III, or hypomethylated pre-tRNAi-Met. Polyadenylates RNA processing and degradation intermediates of snRNAs, snoRNAs and mRNAs that accumulate in strains lacking a functional exosome. TRF5 is also required for proper nuclear division in mitosis and sister chromatid cohesion. Involved in the regulation of histone mRNA levels. May mediate mitotic chromosome condensation. KEYWORDS: ATP-binding;Cell cycle;Cell division;Magnesium;Manganese;Metal-binding;Mitosis;Nucleotide-binding;Nucleotidyltransferase;Nucleus;Phosphoprotein;Reference proteome;Transferase SUBCELLULAR LOCATION: Nucleus, nucleolus MISCELLANEOUS: Present with 2240 molecules/cell in log phase SD medium." P48596,"PROTEIN NAMES: GTP cyclohydrolase 1 (GTP cyclohydrolase I) (GTP-CH-I) (Protein punch) PROTEIN FAMILY: GTP cyclohydrolase I family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the GTP cyclohydrolase I family. FUNCTION: Isoform B is required for eye pigment production, Isoform C may be required for normal embryonic development and segment pattern formation. PATHWAY: Cofactor biosynthesis; 7,8-dihydroneopterin triphosphate biosynthesis; 7,8-dihydroneopterin triphosphate from GTP: step 1/1. KEYWORDS: Allosteric enzyme;Alternative splicing;GTP-binding;Hydrolase;Metal-binding;Nucleotide-binding;Reference proteome;Tetrahydrobiopterin biosynthesis;Zinc " P48635,"PROTEIN NAMES: Erythromycin C-12 hydroxylase (Cytochrome P450 113A1) (CYP113A1) (Erythromycin D C-12 hydroxylase) PROTEIN FAMILY: Cytochrome P450 family ORGANISM: Saccharopolyspora erythraea (strain ATCC 11635 / DSM 40517 / JCM 4748 / NBRC 13426 / NCIMB 8594 / NRRL 2338) SIMILARITY: Belongs to the cytochrome P450 family. FUNCTION: Responsible for the C-12 hydroxylation of the macrolactone ring of erythromycin. Thus, EryK catalyzes the hydroxylation of erythromycin D (ErD) at the C-12 position to produce erythromycin C (ErC). Erythromycin B (ErB) is not a substrate for this enzyme. PATHWAY: Antibiotic biosynthesis; erythromycin biosynthesis. KEYWORDS: 3D-structure;Antibiotic biosynthesis;Heme;Iron;Metal-binding;Monooxygenase;NADP;Oxidoreductase;Reference proteome " P48731,PROTEIN NAMES: Homeobox protein ATH1 PROTEIN FAMILY: TALE/BELL homeobox family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the TALE/BELL homeobox family. FUNCTION: Transcription factor which may be involved in the signal transduction pathway downstream of the COP1 gene. Controls floral competency as a specific activator of FLC expression. Is responsive of the nuclear import of SHOOT MERISTEMLESS (STM). KEYWORDS: DNA-binding;Homeobox;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus P48809,PROTEIN NAMES: Heterogeneous nuclear ribonucleoprotein 27C (Hrb27-C) (HRP48.1) (hnRNP 48) ORGANISM: Drosophila melanogaster (Fruit fly) FUNCTION: This protein is a component of ribonucleosomes. Could be needed to organize a concentration gradient of a dorsalizing morphogen (Dm) originating in the germinal vesicle. KEYWORDS: Cytoplasm;Nucleus;Phosphoprotein;Reference proteome;Repeat;Ribonucleoprotein;RNA-binding SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=Nuclear and/or cytoplasmic. P48831,"PROTEIN NAMES: Zona pellucida sperm-binding protein 3 (Sperm receptor) (Zona pellucida glycoprotein 3) (Zp-3) (Zona pellucida protein C) [Cleaved into: Processed zona pellucida sperm-binding protein 3] PROTEIN FAMILY: ZP domain family, ZPC subfamily ORGANISM: Canis lupus familiaris (Dog) (Canis familiaris) SIMILARITY: Belongs to the ZP domain family. ZPC subfamily. FUNCTION: Component of the zona pellucida, an extracellular matrix surrounding oocytes which mediates sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy. The zona pellucida is composed of 3 to 4 glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP3 is essential for sperm binding and zona matrix formation. KEYWORDS: Cell membrane;Cleavage on pair of basic residues;Disulfide bond;Extracellular matrix;Fertilization;Glycoprotein;Membrane;Pyrrolidone carboxylic acid;Receptor;Reference proteome;Secreted;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: [Processed zona pellucida sperm-binding protein 3]: Zona pellucida.; SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein " P49023,"PROTEIN NAMES: Paxillin PROTEIN FAMILY: Paxillin family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the paxillin family. FUNCTION: Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Recruits other proteins such as TRIM15 to focal adhesion. KEYWORDS: 3D-structure;Acetylation;Alternative splicing;Cell adhesion;Cell junction;Cytoplasm;Cytoskeleton;LIM domain;Metal-binding;Phosphoprotein;Reference proteome;Repeat;Zinc SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton Cell junction, focal adhesion Cytoplasm, cell cortex Note=Colocalizes with integrins at the cell periphery. Colocalize with PXN to membrane ruffles and the leading edge of migrating cells. " P49049,"PROTEIN NAMES: Intramembrane protease 2 PROTEIN FAMILY: Peptidase A22B family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the peptidase A22B family. FUNCTION: Acts as intramembrane protease (Probable). In larvae, required for the complete shedding of the cuticle during molting, possibly by regulating cholesterol uptake via lrp-1. Involved in embryonic and larval development. KEYWORDS: Endoplasmic reticulum;Glycoprotein;Hydrolase;Membrane;Protease;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Multi-pass membrane protein Endoplasmic reticulum membrane ; Multi-pass membrane protein " P49137,"PROTEIN NAMES: MAP kinase-activated protein kinase 2 (MAPK-activated protein kinase 2) (MAPKAP kinase 2) (MAPKAP-K2) (MAPKAPK-2) (MK-2) (MK2) PROTEIN FAMILY: Protein kinase superfamily, CAMK Ser/Thr protein kinase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. FUNCTION: Stress-activated serine/threonine-protein kinase involved in cytokine production, endocytosis, reorganization of the cytoskeleton, cell migration, cell cycle control, chromatin remodeling, DNA damage response and transcriptional regulation. Following stress, it is phosphorylated and activated by MAP kinase p38-alpha/MAPK14, leading to phosphorylation of substrates. Phosphorylates serine in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue. Phosphorylates ALOX5, CDC25B, CDC25C, CEP131, ELAVL1, HNRNPA0, HSP27/HSPB1, KRT18, KRT20, LIMK1, LSP1, PABPC1, PARN, PDE4A, RCSD1, RPS6KA3, TAB3 and TTP/ZFP36. Phosphorylates HSF1; leading to the interaction with HSP90 proteins and inhibiting HSF1 homotrimerization, DNA-binding and transactivation activities. Mediates phosphorylation of HSP27/HSPB1 in response to stress, leading to the dissociation of HSP27/HSPB1 from large small heat-shock protein (sHsps) oligomers and impairment of their chaperone activities and ability to protect against oxidative stress effectively. Involved in inflammatory response by regulating tumor necrosis factor (TNF) and IL6 production post-transcriptionally: acts by phosphorylating AU-rich elements (AREs)-binding proteins ELAVL1, HNRNPA0, PABPC1 and TTP/ZFP36, leading to the regulation of the stability and translation of TNF and IL6 mRNAs. Phosphorylation of TTP/ZFP36, a major post-transcriptional regulator of TNF, promotes its binding to 14-3-3 proteins and reduces its ARE mRNA affinity, leading to inhibition of dependent degradation of ARE-containing transcripts. Phosphorylates CEP131 in response to cellular stress induced by ultraviolet irradiation which promotes binding of CEP131 to 14-3-3 proteins and inhibits formation of novel centriolar satellites. Also involved in late G2/M checkpoint following DNA damage through a process of post-transcriptional mRNA stabilization: following DNA damage, relocalizes from nucleus to cytoplasm and phosphorylates HNRNPA0 and PARN, leading to stabilization of GADD45A mRNA. Involved in toll-like receptor signaling pathway (TLR) in dendritic cells: required for acute TLR-induced macropinocytosis by phosphorylating and activating RPS6KA3. KEYWORDS: 3D-structure;Alternative splicing;ATP-binding;Cytoplasm;DNA damage;Isopeptide bond;Kinase;Nucleotide-binding;Nucleus;Phosphoprotein;Reference proteome;Serine/threonine-protein kinase;Transferase;Ubl conjugation SUBCELLULAR LOCATION: Cytoplasm Nucleus Note=Phosphorylation and subsequent activation releases the autoinhibitory helix, resulting in the export from the nucleus into the cytoplasm. MISCELLANEOUS: [Isoform 1]: Has a nuclear localization signal." P49247,PROTEIN NAMES: Ribose-5-phosphate isomerase (Phosphoriboisomerase) PROTEIN FAMILY: Ribose 5-phosphate isomerase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the ribose 5-phosphate isomerase family. FUNCTION: Catalyzes the reversible conversion of ribose-5-phosphate to ribulose 5-phosphate and participates in the first step of the non-oxidative branch of the pentose phosphate pathway. PATHWAY: Carbohydrate degradation; pentose phosphate pathway; D-ribose 5-phosphate from D-ribulose 5-phosphate (non-oxidative stage): step 1/1. KEYWORDS: Disease variant;Isomerase;Methylation;Neuropathy;Phosphoprotein;Reference proteome P49278,PROTEIN NAMES: Mite group 2 allergen Der p 2 (Allergen Der p II) (DPX) (allergen Der p 2) PROTEIN FAMILY: NPC2 family ORGANISM: Dermatophagoides pteronyssinus (European house dust mite) SIMILARITY: Belongs to the NPC2 family. KEYWORDS: 3D-structure;Allergen;Direct protein sequencing;Disulfide bond;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted. P49321,"PROTEIN NAMES: Nuclear autoantigenic sperm protein (NASP) PROTEIN FAMILY: NASP family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the NASP family. FUNCTION: Required for DNA replication, normal cell cycle progression and cell proliferation. Forms a cytoplasmic complex with HSP90 and H1 linker histones and stimulates HSP90 ATPase activity. NASP and H1 histone are subsequently released from the complex and translocate to the nucleus where the histone is released for binding to DNA. KEYWORDS: 3D-structure;Acetylation;Alternative splicing;Cell cycle;Coiled coil;Cytoplasm;DNA replication;Isopeptide bond;Nucleus;Phosphoprotein;Protein transport;Reference proteome;Repeat;TPR repeat;Transport;Ubl conjugation SUBCELLULAR LOCATION: Cytoplasm Nucleus " P49424,"PROTEIN NAMES: Mannan endo-1,4-beta-mannosidase (Mannanase 26A) (Man26A) (Mannanase A) (ManA) PROTEIN FAMILY: Glycosyl hydrolase 26 family ORGANISM: Cellvibrio japonicus (strain Ueda107) (Pseudomonas fluorescens subsp. cellulosa) SIMILARITY: Belongs to the glycosyl hydrolase 26 family. FUNCTION: Catalyzes the endo hydrolysis of beta-1,4-linked mannan and galactomannan, but displays little activity towards other polysaccharides located in the plant cell wall. Preferentially hydrolyzes the larger oligosaccharides and has greater activity against non-substituted polysaccharides. It displays tight specificity for mannose at both the -2 and the -1 subsites. Appears to act in synergy with alpha-galactosidase (AgaA) to elicit hydrolysis of galactomannan. KEYWORDS: 3D-structure;Carbohydrate metabolism;Direct protein sequencing;Glycosidase;Hydrolase;Polysaccharide degradation;Reference proteome;Signal " P49594,"PROTEIN NAMES: Protein phosphatase fem-2 (Ca(2+)/calmodulin-dependent protein kinase phosphatase) (CaM-kinase phosphatase) (CaMKPase) (Feminization of XX and XO animals protein 2) (Sex-determining protein fem-2) PROTEIN FAMILY: PP2C family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the PP2C family. FUNCTION: Dephosphorylates auto-phosphorylated Ca(2+)/calmodulin-dependent protein kinase unc-43/CAMKII in vitro. Involved in the regulation of sex determination. Together with fem-3, required for male sexual development by promoting the proteasomal-mediated degradation of tra-1, a transcription repressor of male-specific genes. Promotes apoptosis. KEYWORDS: 3D-structure;Apoptosis;Developmental protein;Differentiation;Hydrolase;Magnesium;Manganese;Metal-binding;Protein phosphatase;Reference proteome;Sexual differentiation;Ubl conjugation pathway " P49654,PROTEIN NAMES: P2X purinoceptor 3 (P2X3) (ATP receptor) (Purinergic receptor) PROTEIN FAMILY: P2X receptor family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the P2X receptor family. FUNCTION: Receptor for ATP that acts as a ligand-gated cation channel. Plays a role in sensory perception. Required for normal perception of pain. Required for normal taste perception (By similarity). KEYWORDS: ATP-binding;Cell membrane;Disulfide bond;Glycoprotein;Ion channel;Ion transport;Ligand-gated ion channel;Membrane;Nucleotide-binding;Receptor;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein P49703,"PROTEIN NAMES: ADP-ribosylation factor-like protein 4D (ADP-ribosylation factor-like protein 4L) PROTEIN FAMILY: Small GTPase superfamily, Arf family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the small GTPase superfamily. Arf family. FUNCTION: Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP). GTP-binding protein that does not act as an allosteric activator of the cholera toxin catalytic subunit. Recruits CYTH1, CYTH2, CYTH3 and CYTH4 to the plasma membrane in GDP-bound form. KEYWORDS: Cell membrane;Cytoplasm;GTP-binding;Lipoprotein;Membrane;Myristate;Nucleotide-binding;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus, nucleolus Cell membrane Nucleus Cytoplasm " P49723,PROTEIN NAMES: Ribonucleoside-diphosphate reductase small chain 2 (Ribonucleotide reductase R2 subunit 2) (Ribonucleotide reductase small subunit 2) PROTEIN FAMILY: Ribonucleoside diphosphate reductase small chain family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the ribonucleoside diphosphate reductase small chain family. FUNCTION: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. RNR4 is required for proper folding of RNR2 and assembly with the large subunits. KEYWORDS: 3D-structure;Acetylation;Deoxyribonucleotide synthesis;Direct protein sequencing;Isopeptide bond;Nucleus;Oxidoreductase;Phosphoprotein;Reference proteome;Ubl conjugation SUBCELLULAR LOCATION: Nucleus Note=Found predominantly in the nucleus under normal growth conditions and is redistributed to the cytoplasm in damaged cells in a DNA replication and damage checkpoint-dependent manner. Nuclear localization is mediated by DIF1. MISCELLANEOUS: Present with 88884 molecules/cell in log phase SD medium.; MISCELLANEOUS: Lacks 3 iron-binding residues conserved in all other R2 subunits. P49744,"PROTEIN NAMES: Thrombospondin-4 PROTEIN FAMILY: Thrombospondin family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the thrombospondin family. FUNCTION: Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions and is involved in various processes including cellular proliferation, migration, adhesion and attachment, inflammatory response to CNS injury, regulation of vascular inflammation and adaptive responses of the heart to pressure overload and in myocardial function and remodeling. Binds to structural extracellular matrix (ECM) proteins and modulates the ECM in response to tissue damage, contributing to cardioprotective and adaptive ECM remodeling. Plays a role in ER stress response, via its interaction with the activating transcription factor 6 alpha (ATF6) which produces adaptive ER stress response factors and protects myocardium from pressure overload. May contribute to spinal presynaptic hypersensitivity and neuropathic pain states after peripheral nerve injury. May play a role in regulating protective astrogenesis from the subventricular zone (SVZ) niche after injury in a NOTCH1-dependent manner. KEYWORDS: Calcium;Cell adhesion;Disulfide bond;EGF-like domain;Endoplasmic reticulum;Extracellular matrix;Glycoprotein;Growth factor;Mitogen;Reference proteome;Repeat;Sarcoplasmic reticulum;Secreted;Signal;Tissue remodeling;Unfolded protein response SUBCELLULAR LOCATION: Endoplasmic reticulum Sarcoplasmic reticulum Secreted Secreted, extracellular space Secreted, extracellular space, extracellular matrix " P49767,"PROTEIN NAMES: Vascular endothelial growth factor C (VEGF-C) (Flt4 ligand) (Flt4-L) (Vascular endothelial growth factor-related protein) (VRP) PROTEIN FAMILY: PDGF/VEGF growth factor family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the PDGF/VEGF growth factor family. FUNCTION: Growth factor active in angiogenesis, and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. May function in angiogenesis of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates KDR/VEGFR2 and FLT4/VEGFR3 receptors. KEYWORDS: 3D-structure;Angiogenesis;Cleavage on pair of basic residues;Developmental protein;Differentiation;Direct protein sequencing;Disulfide bond;Glycoprotein;Growth factor;Mitogen;Reference proteome;Repeat;Secreted;Signal SUBCELLULAR LOCATION: Secreted " P49771,PROTEIN NAMES: Fms-related tyrosine kinase 3 ligand (Flt3 ligand) (Flt3L) (SL cytokine) ORGANISM: Homo sapiens (Human) FUNCTION: Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins. KEYWORDS: 3D-structure;Alternative splicing;Cell membrane;Cytokine;Disulfide bond;Glycoprotein;Membrane;Reference proteome;Secreted;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: [Isoform 1]: Cell membrane; Single-pass type I membrane protein.; SUBCELLULAR LOCATION: [Isoform 2]: Secreted. P49777,"PROTEIN NAMES: ARS-binding protein 1 ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) FUNCTION: Binds, preferentially, to the Maundrell ARS consensus sequence within ARS3002. KEYWORDS: 3D-structure;DNA-binding;Nucleus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Nucleus. " P49788,"PROTEIN NAMES: Retinoic acid receptor responder protein 1 (Phorbol ester-induced gene 1 protein) (PERG-1) (RAR-responsive protein TIG1) (Tazarotene-induced gene 1 protein) PROTEIN FAMILY: Protease inhibitor I47 (latexin) family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the protease inhibitor I47 (latexin) family. FUNCTION: Inhibitor of the cytoplasmic carboxypeptidase AGBL2, may regulate the alpha-tubulin tyrosination cycle. KEYWORDS: Alternative splicing;Glycoprotein;Membrane;Protease inhibitor;Proteoglycan;Reference proteome;Repeat;Secreted;Signal-anchor;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Single-pass type III membrane protein Secreted " P49809,"PROTEIN NAMES: Regulator of G-protein signaling egl-10 (Egg-laying defective protein 10) ORGANISM: Caenorhabditis elegans FUNCTION: Involved in egg-laying and locomotion. May regulate G protein goa-1 signaling. Plays a role in regulating dopamine-mediated locomotion behavior. KEYWORDS: Cell projection;Reference proteome;Signal transduction inhibitor SUBCELLULAR LOCATION: Cell projection, dendrite " P49842,PROTEIN NAMES: Inactive serine/threonine-protein kinase 19 (Protein G11) (Protein RP1) PROTEIN FAMILY: STK19 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the STK19 family. FUNCTION: [Isoform 1]: Inactive serine/threonine-protein kinase (Probable). May control NRAS activity via an associated kinase (Probable).; FUNCTION: [Isoform 3]: Inactive serine/threonine-protein kinase. KEYWORDS: 3D-structure;Alternative splicing;ATP-binding;Cytoplasm;Kinase;Manganese;Nucleotide-binding;Nucleus;Reference proteome;Serine/threonine-protein kinase;Transferase SUBCELLULAR LOCATION: [Isoform 3]: Nucleus Note=Very tightly chromatin-associated.; SUBCELLULAR LOCATION: [Isoform 1]: Nucleus Cytoplasm P49866,"PROTEIN NAMES: Transcription factor HNF-4 homolog (dHNF4) (Nuclear receptor subfamily 2 group A member 4) PROTEIN FAMILY: Nuclear hormone receptor family, NR2 subfamily ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the nuclear hormone receptor family. NR2 subfamily. FUNCTION: Transcriptionally controlled transcription factor. Important for the differentiation of various specialized cell types that arise from both endoderm and mesoderm. May have a role in early gut formation. Plays an essential role in lipid catabolism, regulating lipid mobilization and beta-oxidation in response to nutrient deprivation. KEYWORDS: Alternative splicing;DNA-binding;Lipid degradation;Lipid metabolism;Metal-binding;Nucleus;Receptor;Reference proteome;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus " P49880,"PROTEIN NAMES: Ecdysone receptor (20-hydroxy-ecdysone receptor) (20E receptor) (EcRH) (AaEcR) (Ecdysteroid receptor) (Nuclear receptor subfamily 1 group H member 1) PROTEIN FAMILY: Nuclear hormone receptor family, NR1 subfamily ORGANISM: Aedes aegypti (Yellowfever mosquito) (Culex aegypti) SIMILARITY: Belongs to the nuclear hormone receptor family. NR1 subfamily. FUNCTION: Receptor for ecdysone. Binds to ecdysone response elements (ECRES). KEYWORDS: Alternative splicing;DNA-binding;Metal-binding;Nucleus;Receptor;Reference proteome;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus. " P49910,PROTEIN NAMES: Zinc finger protein 165 (Cancer/testis antigen 53) (CT53) (LD65) (Zinc finger and SCAN domain-containing protein 7) PROTEIN FAMILY: Krueppel C2H2-type zinc-finger protein family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein family. FUNCTION: May be involved in transcriptional regulation. KEYWORDS: DNA-binding;Isopeptide bond;Metal-binding;Nucleus;Reference proteome;Repeat;Transcription;Transcription regulation;Ubl conjugation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus P49919,"PROTEIN NAMES: Cyclin-dependent kinase inhibitor 1C (Cyclin-dependent kinase inhibitor p57) (p57Kip2) PROTEIN FAMILY: CDI family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the CDI family. FUNCTION: Potent tight-binding inhibitor of several G1 cyclin/CDK complexes (cyclin E-CDK2, cyclin D2-CDK4, and cyclin A-CDK2) and, to lesser extent, of the mitotic cyclin B-CDC2. Negative regulator of cell proliferation. May play a role in maintenance of the non-proliferative state throughout life. KEYWORDS: Alternative splicing;Cell cycle;Methylation;Nucleus;Protein kinase inhibitor;Reference proteome SUBCELLULAR LOCATION: Nucleus. " P49956,"PROTEIN NAMES: Chromosome transmission fidelity protein 18 PROTEIN FAMILY: Activator 1 small subunits family, CTF18 subfamily ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the activator 1 small subunits family. CTF18 subfamily. FUNCTION: Essential for the fidelity of chromosome transmission. Required for the DNA replication block checkpoint. Component of the RFC-like complex CTF18-RFC which is required for efficient establishment of chromosome cohesion during S-phase and may load or unload POL30/PCNA. During a clamp loading circle, the RFC:clamp complex binds to DNA and the recognition of the double-stranded/single-stranded junction stimulates ATP hydrolysis by RFC. The complex presumably provides bipartite ATP sites in which one subunit supplies a catalytic site for hydrolysis of ATP bound to the neighboring subunit. Dissociation of RFC from the clamp leaves the clamp encircling DNA. KEYWORDS: 3D-structure;ATP-binding;Cell cycle;DNA replication;DNA-binding;Nucleotide-binding;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus " P50090,PROTEIN NAMES: Kelch repeat-containing protein 2 ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) KEYWORDS: Coiled coil;Kelch repeat;Phosphoprotein;Reference proteome;Repeat MISCELLANEOUS: Present with 468 molecules/cell in log phase SD medium. P50102,"PROTEIN NAMES: Ubiquitin carboxyl-terminal hydrolase 8 (Deubiquitinating enzyme 8) (Ubiquitin thioesterase 8) (Ubiquitin-specific-processing protease 8) PROTEIN FAMILY: Peptidase C19 family, UBP8 subfamily ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the peptidase C19 family. UBP8 subfamily. FUNCTION: Functions as a histone deubiquitinating component of the transcription regulatory histone acetylation (HAT) complexes SAGA and SLIK. SAGA is involved in RNA polymerase II-dependent transcriptional regulation of approximately 10% of yeast genes. At the promoters, SAGA is required for recruitment of the basal transcription machinery. It influences RNA polymerase II transcriptional activity through different activities such as TBP interaction (SPT3, SPT8 and SPT20) and promoter selectivity, interaction with transcription activators (GCN5, ADA2, ADA3 and TRA1), and chromatin modification through histone acetylation (GCN5) and deubiquitination (UBP8). SAGA acetylates nucleosomal histone H3 to some extent (to form H3K9ac, H3K14ac, H3K18ac and H3K23ac). SAGA interacts with DNA via upstream activating sequences (UASs). SLIK is proposed to have partly overlapping functions with SAGA. It preferentially acetylates methylated histone H3, at least after activation at the GAL1-10 locus. Together with SGF11, is required for histone H2B deubiquitination. KEYWORDS: 3D-structure;Hydrolase;Metal-binding;Nucleus;Protease;Reference proteome;Thiol protease;Transcription;Transcription regulation;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: Present with 1030 molecules/cell in log phase SD medium." P50108,"PROTEIN NAMES: Probable alpha-1,6-mannosyltransferase MNN10 (Bud emergence delay protein 1) (Mannan polymerase II complex MNN10 subunit) (M-Pol II subunit MNN10) PROTEIN FAMILY: Glycosyltransferase 34 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the glycosyltransferase 34 family. FUNCTION: Required for polarized growth and efficient budding.; FUNCTION: The M-Pol II complex possesses alpha-1,6-mannosyltransferase activity and is probably involved in the elongation of the mannan backbone of N-linked glycans on cell wall and periplasmic proteins. KEYWORDS: Endoplasmic reticulum;Glycosyltransferase;Golgi apparatus;Membrane;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Single-pass type II membrane protein. Golgi apparatus, cis-Golgi network membrane; Single-pass type II membrane protein. MISCELLANEOUS: Present with 6280 molecules/cell in log phase SD medium." P50168,"PROTEIN NAMES: 11-beta-hydroxysteroid dehydrogenase type 2 (11-DH2) (11-beta-HSD2) (Corticosteroid 11-beta-dehydrogenase isozyme 2) (NAD-dependent 11-beta-hydroxysteroid dehydrogenase) PROTEIN FAMILY: Short-chain dehydrogenases/reductases (SDR) family ORGANISM: Ovis aries (Sheep) SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FUNCTION: Catalyzes the conversion of biologically active 11beta-hydroxyglucocorticoids (11beta-hydroxysteroid) such as cortisol, to inactive 11-ketoglucocorticoids (11-oxosteroid) such as cortisone, in the presence of NAD(+). Functions as a dehydrogenase (oxidase), thereby decreasing the concentration of active glucocorticoids, thus protecting the nonselective mineralocorticoid receptor from occupation by glucocorticoids. Plays an important role in maintaining glucocorticoids balance during preimplantation and protects the fetus from excessive maternal corticosterone exposure (By similarity). Catalyzes the oxidation of 11beta-hydroxytestosterone (11beta,17beta-dihydroxyandrost-4-ene-3-one) to 11-ketotestosterone (17beta-hydroxyandrost-4-ene-3,11-dione), a major bioactive androgen.Catalyzes the conversion of 11beta-hydroxyandrostenedione (11beta-hydroxyandrost-4-ene-3,17-dione) to 11-ketoandrostenedione (androst-4-ene-3,11,17-trione), which can be further metabolized to 11-ketotestosterone. Converts 7-beta-25-dihydroxycholesterol to 7-oxo-25-hydroxycholesterol in vitro. 7-beta-25-dihydroxycholesterol (not 7-oxo-25-hydroxycholesterol) acts as a ligand for the G-protein-coupled receptor (GPCR) Epstein-Barr virus-induced gene 2 (EBI2) and may thereby regulate immune cell migration (By similarity). May protect ovulating oocytes and fertilizing spermatozoa from the adverse effects of cortisol (By similarity). PATHWAY: Steroid metabolism. KEYWORDS: Endoplasmic reticulum;Lipid metabolism;Microsome;NAD;Oxidoreductase;Reference proteome;Steroid metabolism SUBCELLULAR LOCATION: Microsome Endoplasmic reticulum " P50389,"PROTEIN NAMES: Purine nucleoside phosphorylase (PNP) (5'-methylthioadenosine phosphorylase I) (MTA phosphorylase I) (MTAPI) PROTEIN FAMILY: PNP/UDP phosphorylase family ORGANISM: Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus) SIMILARITY: Belongs to the PNP/UDP phosphorylase family. FUNCTION: Cleavage of guanosine or inosine to respective bases and sugar-1-phosphate molecules. Cleaves inosine, guanosine, and adenosine with a better efficiency than MTA. PATHWAY: Purine metabolism; purine nucleoside salvage. KEYWORDS: 3D-structure;Direct protein sequencing;Disulfide bond;Glycosyltransferase;Reference proteome;Transferase " P50440,"PROTEIN NAMES: Glycine amidinotransferase, mitochondrial (L-arginine:glycine amidinotransferase) (Transamidinase) PROTEIN FAMILY: Amidinotransferase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the amidinotransferase family. FUNCTION: Transamidinase that catalyzes the transfer of the amidino group of L-arginine onto the amino moiety of acceptor metabolites such as glycine, beta-alanine, gamma-aminobutyric acid (GABA) and taurine yielding the corresponding guanidine derivatives. Catalyzes the rate-limiting step of creatine biosynthesis, namely the transfer of the amidino group from L-arginine to glycine to generate guanidinoacetate, which is then methylated by GAMT to form creatine. Provides creatine as a source for ATP generation in tissues with high energy demands, in particular skeletal muscle, heart and brain (Probable). PATHWAY: Amine and polyamine biosynthesis; creatine biosynthesis; creatine from L-arginine and glycine: step 1/2. KEYWORDS: 3D-structure;Acetylation;Alternative splicing;Cytoplasm;Disease variant;Membrane;Mitochondrion;Mitochondrion inner membrane;Phosphoprotein;Reference proteome;Transferase;Transit peptide SUBCELLULAR LOCATION: [Isoform 1]: Mitochondrion inner membrane; Peripheral membrane protein; Intermembrane side. Note=Probably attached to the outer side of the inner membrane.; SUBCELLULAR LOCATION: [Isoform 2]: Cytoplasm. " P50455,PROTEIN NAMES: 3-isopropylmalate dehydrogenase (3-IPM-DH) (IMDH) (Beta-IPM dehydrogenase) PROTEIN FAMILY: Isocitrate and isopropylmalate dehydrogenases family ORGANISM: Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) SIMILARITY: Belongs to the isocitrate and isopropylmalate dehydrogenases family. FUNCTION: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate. PATHWAY: Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 3/4. KEYWORDS: 3D-structure;Amino-acid biosynthesis;Branched-chain amino acid biosynthesis;Cytoplasm;Direct protein sequencing;Leucine biosynthesis;Magnesium;Manganese;Metal-binding;NAD;Oxidoreductase;Reference proteome SUBCELLULAR LOCATION: Cytoplasm. P50537,PROTEIN NAMES: Malic acid transport protein (Malate permease) PROTEIN FAMILY: Tellurite-resistance/dicarboxylate transporter (TDT) family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the tellurite-resistance/dicarboxylate transporter (TDT) family. FUNCTION: Permease for malate and other C4 dicarboxylic acids. KEYWORDS: Membrane;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. P50544,"PROTEIN NAMES: Very long-chain specific acyl-CoA dehydrogenase, mitochondrial (MVLCAD) (VLCAD) PROTEIN FAMILY: Acyl-CoA dehydrogenase family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the acyl-CoA dehydrogenase family. FUNCTION: Very long-chain specific acyl-CoA dehydrogenase is one of the acyl-CoA dehydrogenases that catalyze the first step of mitochondrial fatty acid beta-oxidation, an aerobic process breaking down fatty acids into acetyl-CoA and allowing the production of energy from fats. The first step of fatty acid beta-oxidation consists in the removal of one hydrogen from C-2 and C-3 of the straight-chain fatty acyl-CoA thioester, resulting in the formation of trans-2-enoyl-CoA. Among the different mitochondrial acyl-CoA dehydrogenases, very long-chain specific acyl-CoA dehydrogenase acts specifically on acyl-CoAs with saturated 12 to 24 carbons long primary chains. PATHWAY: Lipid metabolism; mitochondrial fatty acid beta-oxidation. KEYWORDS: 3D-structure;Acetylation;FAD;Fatty acid metabolism;Flavoprotein;Lipid metabolism;Membrane;Mitochondrion;Mitochondrion inner membrane;Oxidoreductase;Phosphoprotein;Reference proteome;S-nitrosylation;Transit peptide SUBCELLULAR LOCATION: Mitochondrion inner membrane ; Peripheral membrane protein " P50592,"PROTEIN NAMES: Tumor necrosis factor ligand superfamily member 10 (TNF-related apoptosis-inducing ligand) (Protein TRAIL) (CD antigen CD253) PROTEIN FAMILY: Tumor necrosis factor family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the tumor necrosis factor family. FUNCTION: Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis. KEYWORDS: Apoptosis;Cell membrane;Cytokine;Glycoprotein;Membrane;Metal-binding;Phosphoprotein;Reference proteome;Secreted;Signal-anchor;Transmembrane;Transmembrane helix;Zinc SUBCELLULAR LOCATION: Cell membrane ; Single-pass type II membrane protein Secreted Note=Exists both as membrane-bound and soluble form. " P50607,"PROTEIN NAMES: Tubby protein homolog PROTEIN FAMILY: TUB family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the TUB family. FUNCTION: Functions in signal transduction from heterotrimeric G protein-coupled receptors. Binds to membranes containing phosphatidylinositol 4,5-bisphosphate. Can bind DNA (in vitro). May contribute to the regulation of transcription in the nucleus. Could be involved in the hypothalamic regulation of body weight (By similarity). Contribute to stimulation of phagocytosis of apoptotic retinal pigment epithelium (RPE) cells and macrophages. KEYWORDS: 3D-structure;Alternative splicing;Cell membrane;Cytoplasm;Membrane;Nucleus;Obesity;Phagocytosis;Reference proteome;Secreted;Sensory transduction SUBCELLULAR LOCATION: Cytoplasm Nucleus Secreted Cell membrane ; Peripheral membrane protein ; Cytoplasmic side Note=Binds phospholipid and is anchored to the plasma membrane through binding phosphatidylinositol 4,5-bisphosphate. Is released upon activation of phospholipase C. Translocates from the plasma membrane to the nucleus upon activation of guanine nucleotide-binding protein G(q) subunit alpha. Does not have a cleavable signal peptide and is secreted by a non-conventional pathway (By similarity). " P50636,"PROTEIN NAMES: E3 ubiquitin-protein ligase RNF19A (Double ring-finger protein) (Dorfin) (Gametogenesis-expressed protein GEG-154) (RING finger protein 19A) (UBCM4-interacting protein 117) (UIP117) (XY body protein) (XYbp) PROTEIN FAMILY: RBR family, RNF19 subfamily ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the RBR family. RNF19 subfamily. FUNCTION: E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates, such as SNCAIP or CASR. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Cytoplasm;Cytoskeleton;Membrane;Metal-binding;Phosphoprotein;Reference proteome;Repeat;Transferase;Transmembrane;Transmembrane helix;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Membrane ; Multi-pass membrane protein Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Note=Expressed primarily in the XY body of pachytene spermatocytes and in the centrosome of somatic and germ cells in all phases of the cell cycle. " P50747,"PROTEIN NAMES: Biotin--protein ligase (Biotin apo-protein ligase) [Includes: Biotin--[methylmalonyl-CoA-carboxytransferase] ligase ; Biotin--[propionyl-CoA-carboxylase [ATP-hydrolyzing]] ligase (Holocarboxylase synthetase) (HCS); Biotin--[methylcrotonoyl-CoA-carboxylase] ligase ; Biotin--[acetyl-CoA-carboxylase] ligase ] PROTEIN FAMILY: Biotin--protein ligase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the biotin--protein ligase family. FUNCTION: Biotin--protein ligase catalyzing the biotinylation of the 4 biotin-dependent carboxylases acetyl-CoA-carboxylase, pyruvate carboxylase, propionyl-CoA carboxylase, and methylcrotonyl-CoA carboxylase. KEYWORDS: Alternative splicing;ATP-binding;Cytoplasm;Disease variant;Ligase;Mitochondrion;Multifunctional enzyme;Nucleotide-binding;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Mitochondrion " P50749,"PROTEIN NAMES: Ras association domain-containing protein 2 ORGANISM: Homo sapiens (Human) FUNCTION: Potential tumor suppressor. Acts as a KRAS-specific effector protein. May promote apoptosis and cell cycle arrest. Stabilizes STK3/MST2 by protecting it from proteasomal degradation. KEYWORDS: Alternative splicing;Cell cycle;Centromere;Chromosome;Cytoplasm;Kinetochore;Nucleus;Phosphoprotein;Reference proteome;Tumor suppressor SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Chromosome, centromere, kinetochore Note=Translocates to the cytoplasm in the presence of STK3/MST2 and STK4/MST1. " P50895,PROTEIN NAMES: Basal cell adhesion molecule (Auberger B antigen) (B-CAM cell surface glycoprotein) (F8/G253 antigen) (Lutheran antigen) (Lutheran blood group glycoprotein) (CD antigen CD239) ORGANISM: Homo sapiens (Human) FUNCTION: Laminin alpha-5 receptor. May mediate intracellular signaling. KEYWORDS: 3D-structure;Blood group antigen;Cell adhesion;Direct protein sequencing;Disulfide bond;Glycoprotein;Immunoglobulin domain;Membrane;Phosphoprotein;Receptor;Reference proteome;Repeat;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. P51131,"PROTEIN NAMES: Cytochrome b/c1 [Cleaved into: Cytochrome b; Cytochrome c1] PROTEIN FAMILY: Cytochrome b family ORGANISM: Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110) SIMILARITY: Belongs to the cytochrome b family. FUNCTION: Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis. c1 functions as an electron donor to cytochrome c. KEYWORDS: Cell inner membrane;Cell membrane;Direct protein sequencing;Electron transport;Heme;Iron;Membrane;Metal-binding;Reference proteome;Respiratory chain;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell inner membrane ; Multi-pass membrane protein " P51485,"PROTEIN NAMES: Beta-arrestin arr-1 PROTEIN FAMILY: Arrestin family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the arrestin family. FUNCTION: Adapter protein required for olfactory adaptation and recovery to volatile odorants, probably by desensitization of G-protein coupled receptors (GPCR). May play a role in clathrin-mediated GPCR endocytosis. Acts as a positive regulator of insulin-like daf-2 signaling pathway probably by forming a complex with mpz-1 and phosphatase daf-18 likely resulting in daf-18 inhibition. Involved in egg-laying. KEYWORDS: Cell projection;Reference proteome;Sensory transduction SUBCELLULAR LOCATION: Perikaryon Cell projection, dendrite Note=Localizes to the amphid processes of chemosensory neurons. " P51511,PROTEIN NAMES: Matrix metalloproteinase-15 (MMP-15) (Membrane-type matrix metalloproteinase 2) (MT-MMP 2) (MTMMP2) (Membrane-type-2 matrix metalloproteinase) (MT2-MMP) (MT2MMP) (SMCP-2) PROTEIN FAMILY: Peptidase M10A family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the peptidase M10A family. FUNCTION: Endopeptidase that degrades various components of the extracellular matrix. May activate progelatinase A. KEYWORDS: Calcium;Cleavage on pair of basic residues;Disulfide bond;Glycoprotein;Hydrolase;Membrane;Metal-binding;Metalloprotease;Phosphoprotein;Protease;Reference proteome;Repeat;Signal;Transmembrane;Transmembrane helix;Zinc;Zymogen SUBCELLULAR LOCATION: Membrane ; Single-pass type I membrane protein ; Extracellular side P51559,"PROTEIN NAMES: Endoprotease bli-4 (Blisterase) (Blistered cuticle protein 4) PROTEIN FAMILY: Peptidase S8 family, Furin subfamily ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the peptidase S8 family. Furin subfamily. FUNCTION: Serine endoprotease which cleaves proproteins at paired basic amino acids (Probable). Involved in cuticle biosynthesis probably by cleaving pro-collagen into its mature form. Acts in ASEL sensory neurons to regulate high salt chemotaxis responses probably by cleaving insulin-like protein ins-6 into its mature and active form. Essential for embryonic and larval development. isoform a, isoform e, isoform f, isoform g and isoform h are involved in cuticle biosynthesis but are dispensable for larval development. KEYWORDS: Alternative splicing;Calcium;Cleavage on pair of basic residues;Disulfide bond;Glycoprotein;Hydrolase;Membrane;Metal-binding;Protease;Reference proteome;Repeat;Serine protease;Signal;Transmembrane;Transmembrane helix;Zymogen SUBCELLULAR LOCATION: Membrane ; Single-pass type I membrane protein " P51578,"PROTEIN NAMES: P2X purinoceptor 5 (P2X5) (ATP receptor) (Purinergic receptor) PROTEIN FAMILY: P2X receptor family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the P2X receptor family. FUNCTION: ATP-gated nonselective transmembrane cation channel. Permeable to potassium, sodium and calcium (By similarity). Unlike, other P2RX receptors, the P2X5 receptor is also permeable to chloride (By similarity). Acts as an important regulator of inflammatory-related bone loss and osteoclast multinucleation (By similarity). KEYWORDS: ATP-binding;Cell membrane;Disulfide bond;Glycoprotein;Ion channel;Ion transport;Ligand-gated ion channel;Membrane;Nucleotide-binding;Receptor;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein " P51648,PROTEIN NAMES: Aldehyde dehydrogenase family 3 member A2 (Aldehyde dehydrogenase 10) (Fatty aldehyde dehydrogenase) (Microsomal aldehyde dehydrogenase) PROTEIN FAMILY: Aldehyde dehydrogenase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the aldehyde dehydrogenase family. FUNCTION: Catalyzes the oxidation of medium and long chain aliphatic aldehydes to fatty acids. Active on a variety of saturated and unsaturated aliphatic aldehydes between 6 and 24 carbons in length. Responsible for conversion of the sphingosine 1-phosphate (S1P) degradation product hexadecenal to hexadecenoic acid. KEYWORDS: 3D-structure;Alternative splicing;Direct protein sequencing;Disease variant;Endoplasmic reticulum;Fatty acid metabolism;Ichthyosis;Intellectual disability;Lipid metabolism;Membrane;Microsome;NAD;Oxidoreductase;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Microsome membrane ; Single-pass membrane protein Endoplasmic reticulum membrane ; Single-pass membrane protein ; Cytoplasmic side P51684,"PROTEIN NAMES: C-C chemokine receptor type 6 (C-C CKR-6) (CC-CKR-6) (CCR-6) (Chemokine receptor-like 3) (CKR-L3) (DRY6) (G-protein coupled receptor 29) (GPR-CY4) (GPRCY4) (LARC receptor) (CD antigen CD196) PROTEIN FAMILY: G-protein coupled receptor 1 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the G-protein coupled receptor 1 family. FUNCTION: Receptor for the C-C type chemokine CCL20. Binds to CCL20 and subsequently transduces a signal by increasing the intracellular calcium ion levels. Although CCL20 is its major ligand it can also act as a receptor for non-chemokine ligands such as beta-defensins. Binds to defensin DEFB1 leading to increase in intracellular calcium ions and cAMP levels. Its binding to DEFB1 is essential for the function of DEFB1 in regulating sperm motility and bactericidal activity. Binds to defensins DEFB4 and DEFB4A/B and mediates their chemotactic effects. The ligand-receptor pair CCL20-CCR6 is responsible for the chemotaxis of dendritic cells (DC), effector/ memory T-cells and B-cells and plays an important role at skin and mucosal surfaces under homeostatic and inflammatory conditions, as well as in pathology, including cancer and various autoimmune diseases. CCR6-mediated signals are essential for immune responses to microbes in the intestinal mucosa and in the modulation of inflammatory responses initiated by tissue insult and trauma. CCR6 is essential for the recruitment of both the pro-inflammatory IL17 producing helper T-cells (Th17) and the regulatory T-cells (Treg) to sites of inflammation. Required for the normal migration of Th17 cells in Peyers-patches and other related tissue sites of the intestine and plays a role in regulating effector T-cell balance and distribution in inflamed intestine. Plays an important role in the coordination of early thymocyte precursor migration events important for normal subsequent thymocyte precursor development, but is not required for the formation of normal thymic natural regulatory T-cells (nTregs). Required for optimal differentiation of DN2 and DN3 thymocyte precursors. Essential for B-cell localization in the subepithelial dome of Peyers-patches and for efficient B-cell isotype switching to IgA in the Peyers-patches. Essential for appropriate anatomical distribution of memory B-cells in the spleen and for the secondary recall response of memory B-cells (By similarity). Positively regulates sperm motility and chemotaxis via its binding to CCL20. KEYWORDS: 3D-structure;Cell membrane;Disulfide bond;G-protein coupled receptor;Glycoprotein;Membrane;Receptor;Reference proteome;Transducer;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Cell surface " P51809,"PROTEIN NAMES: Vesicle-associated membrane protein 7 (VAMP-7) (Synaptobrevin-like protein 1) (Tetanus-insensitive VAMP) (Ti-VAMP) PROTEIN FAMILY: Synaptobrevin family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the synaptobrevin family. FUNCTION: Involved in the targeting and/or fusion of transport vesicles to their target membrane during transport of proteins from the early endosome to the lysosome. Required for heterotypic fusion of late endosomes with lysosomes and homotypic lysosomal fusion. Required for calcium regulated lysosomal exocytosis. Involved in the export of chylomicrons from the endoplasmic reticulum to the cis Golgi. Required for exocytosis of mediators during eosinophil and neutrophil degranulation, and target cell killing by natural killer cells. Required for focal exocytosis of late endocytic vesicles during phagosome formation. KEYWORDS: 3D-structure;Acetylation;Alternative splicing;Coiled coil;Cytoplasmic vesicle;Direct protein sequencing;Endoplasmic reticulum;Endosome;Exocytosis;Golgi apparatus;Lysosome;Membrane;Phosphoprotein;Protein transport;Reference proteome;Signal-anchor;Synapse;Synaptosome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle membrane ; Single-pass type IV membrane protein Golgi apparatus, trans-Golgi network membrane ; Single-pass type IV membrane protein Late endosome membrane ; Single-pass type IV membrane protein Lysosome membrane ; Single-pass type IV membrane protein Endoplasmic reticulum membrane ; Single-pass type IV membrane protein Cytoplasmic vesicle, phagosome membrane ; Single-pass type IV membrane protein Synapse, synaptosome Note=In immature neurons expression is localized in vesicular structures in axons and dendrites while in mature neurons it is localized to the somatodendritic region. Colocalizes with LAMP1 in kidney cells. Localization to the endoplasmic reticulum membrane was observed in the intestine but not in liver or kidney (By similarity). MISCELLANEOUS: The gene coding for this protein is located in the pseudoautosomal region 2 (PAR2) of X and Y chromosomes.; MISCELLANEOUS: Loss-of-function mutant (antisense inhibition) displays impaired granzyme B release and target cell killing by natural killer cells." P51813,"PROTEIN NAMES: Cytoplasmic tyrosine-protein kinase BMX (Bone marrow tyrosine kinase gene in chromosome X protein) (Epithelial and endothelial tyrosine kinase) (ETK) (NTK38) PROTEIN FAMILY: Protein kinase superfamily, Tyr protein kinase family, TEC subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily. FUNCTION: Non-receptor tyrosine kinase that plays central but diverse modulatory roles in various signaling processes involved in the regulation of actin reorganization, cell migration, cell proliferation and survival, cell adhesion, and apoptosis. Participates in signal transduction stimulated by growth factor receptors, cytokine receptors, G-protein coupled receptors, antigen receptors and integrins. Induces tyrosine phosphorylation of BCAR1 in response to integrin regulation. Activation of BMX by integrins is mediated by PTK2/FAK1, a key mediator of integrin signaling events leading to the regulation of actin cytoskeleton and cell motility. Plays a critical role in TNF-induced angiogenesis, and implicated in the signaling of TEK and FLT1 receptors, 2 important receptor families essential for angiogenesis. Required for the phosphorylation and activation of STAT3, a transcription factor involved in cell differentiation. Also involved in interleukin-6 (IL6) induced differentiation. Also plays a role in programming adaptive cytoprotection against extracellular stress in different cell systems, salivary epithelial cells, brain endothelial cells, and dermal fibroblasts. May be involved in regulation of endocytosis through its interaction with an endosomal protein RUFY1. May also play a role in the growth and differentiation of hematopoietic cells; as well as in signal transduction in endocardial and arterial endothelial cells. KEYWORDS: 3D-structure;Apoptosis;ATP-binding;Cell adhesion;Cytoplasm;Direct protein sequencing;Kinase;Metal-binding;Nucleotide-binding;Phosphoprotein;Reference proteome;SH2 domain;Stress response;Transferase;Tyrosine-protein kinase;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cytoplasm Note=Localizes to the edges of spreading cells when complexed with BCAR1. " P51860,"PROTEIN NAMES: Nucleosome assembly protein 1-like 2 (Brain-specific protein, X-linked) PROTEIN FAMILY: Nucleosome assembly protein (NAP) family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the nucleosome assembly protein (NAP) family. FUNCTION: Acidic protein which may be involved in interactions with other proteins or DNA. KEYWORDS: Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus " P51912,"PROTEIN NAMES: Neutral amino acid transporter B(0) (ATB(0)) (ASC-like Na(+)-dependent neutral amino acid transporter ASCT2) (Insulin-activated amino acid transporter) (Sodium-dependent neutral amino acid transporter type 2) (Solute carrier family 1 member 5) PROTEIN FAMILY: Dicarboxylate/amino acid:cation symporter (DAACS) family, SLC1A5 subfamily ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the dicarboxylate/amino acid:cation symporter (DAACS) (TC 2.A.23) family. SLC1A5 subfamily. FUNCTION: Sodium-coupled antiporter of neutral amino acids. In a tri-substrate transport cycle, exchanges neutral amino acids between the extracellular and intracellular compartments, coupled to the inward cotransport of at least one sodium ion (By similarity). The preferred substrate is the essential amino acid L-glutamine, a precursor for biosynthesis of proteins, nucleotides and amine sugars as well as an alternative fuel for mitochondrial oxidative phosphorylation. Exchanges L-glutamine with other neutral amino acids such as L-serine, L-threonine and L-asparagine in a bidirectional way. Provides L-glutamine to proliferating stem and activated cells driving the metabolic switch toward cell differentiation (By similarity). The transport cycle is usually pH-independent, with the exception of L-glutamate. Transports extracellular L-glutamate coupled to the cotransport of one proton and one sodium ion in exchange for intracellular L-glutamine counter-ion. May provide for L-glutamate uptake in glial cells regulating glutamine/glutamate cycle in the nervous system (By similarity). Can transport D-amino acids. Mediates D-serine release from the retinal glia potentially affecting NMDA receptor function in retinal neurons (By similarity). Displays sodium- and amino acid-dependent but uncoupled channel-like anion conductance with a preference SCN(-) >> NO3(-) > I(-) > Cl(-) (By similarity). Through binding of the fusogenic protein syncytin-1/ERVW-1 may mediate trophoblasts syncytialization, the spontaneous fusion of their plasma membranes, an essential process in placental development (By similarity). KEYWORDS: Acetylation;Amino-acid transport;Antiport;Cell membrane;Glycoprotein;Membrane;Metal-binding;Phosphoprotein;Reference proteome;Sodium;Symport;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Melanosome " P51946,"PROTEIN NAMES: Cyclin-H (MO15-associated protein) (p34) (p37) PROTEIN FAMILY: Cyclin family, Cyclin C subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the cyclin family. Cyclin C subfamily. FUNCTION: Regulates CDK7, the catalytic subunit of the CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle. KEYWORDS: 3D-structure;Cell cycle;Cyclin;Direct protein sequencing;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus. " P51955,"PROTEIN NAMES: Serine/threonine-protein kinase Nek2 (HSPK 21) (Never in mitosis A-related kinase 2) (NimA-related protein kinase 2) (NimA-like protein kinase 1) PROTEIN FAMILY: Protein kinase superfamily, NEK Ser/Thr protein kinase family, NIMA subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily. FUNCTION: Protein kinase which is involved in the control of centrosome separation and bipolar spindle formation in mitotic cells and chromatin condensation in meiotic cells. Regulates centrosome separation (essential for the formation of bipolar spindles and high-fidelity chromosome separation) by phosphorylating centrosomal proteins such as CROCC, CEP250 and NINL, resulting in their displacement from the centrosomes. Regulates kinetochore microtubule attachment stability in mitosis via phosphorylation of NDC80. Involved in regulation of mitotic checkpoint protein complex via phosphorylation of CDC20 and MAD2L1. Plays an active role in chromatin condensation during the first meiotic division through phosphorylation of HMGA2. Phosphorylates: PPP1CC; SGO1; NECAB3 and NPM1. Essential for localization of MAD2L1 to kinetochore and MAPK1 and NPM1 to the centrosome. Phosphorylates CEP68 and CNTLN directly or indirectly. NEK2-mediated phosphorylation of CEP68 promotes CEP68 dissociation from the centrosome and its degradation at the onset of mitosis. Involved in the regulation of centrosome disjunction. Phosphorylates CCDC102B either directly or indirectly which causes CCDC102B to dissociate from the centrosome and allows for centrosome separation.; FUNCTION: [Isoform 1]: Phosphorylates and activates NEK11 in G1/S-arrested cells.; FUNCTION: [Isoform 2]: Not present in the nucleolus and, in contrast to isoform 1, does not phosphorylate and activate NEK11 in G1/S-arrested cells. KEYWORDS: 3D-structure;Alternative splicing;ATP-binding;Cell cycle;Cell division;Centromere;Chromosome;Chromosome partition;Coiled coil;Cytoplasm;Cytoskeleton;Kinase;Kinetochore;Magnesium;Meiosis;Metal-binding;Microtubule;Mitosis;Nucleotide-binding;Nucleus;Phosphoprotein;Reference proteome;Retinitis pigmentosa;Serine/threonine-protein kinase;Transferase SUBCELLULAR LOCATION: [Isoform 1]: Nucleus. Nucleus, nucleolus Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Cytoplasm, cytoskeleton, spindle pole. Chromosome, centromere, kinetochore. Chromosome, centromere Note=STK3/MST2 and SAV1 are required for its targeting to the centrosome. Colocalizes with SGO1 and MAD1L1 at the kinetochore. Not associated with kinetochore in the interphase but becomes associated with it upon the breakdown of the nuclear envelope. Has a nucleolar targeting/ retention activity via a coiled-coil domain at the C-terminal end.; SUBCELLULAR LOCATION: [Isoform 2]: Cytoplasm. Note=Predominantly cytoplasmic.; SUBCELLULAR LOCATION: [Isoform 4]: Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Predominantly nuclear. " P52012,"PROTEIN NAMES: Peptidyl-prolyl cis-trans isomerase 4 (PPIase 4) (Cyclophilin mog-6) (Cyclophilin-4) (Masculinization of germline protein 6) (RING-type E3 ubiquitin transferase isomerase 4) (Rotamase 4) PROTEIN FAMILY: Cyclophilin-type PPIase family, PPIL2 subfamily ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the cyclophilin-type PPIase family. PPIL2 subfamily. FUNCTION: May catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides thereby assisting the folding of proteins. May also function as a chaperone, playing a role in intracellular transport of proteins. May also have a protein ubiquitin ligase activity acting as an E3 ubiquitin protein ligase or as a ubiquitin-ubiquitin ligase promoting elongation of ubiquitin chains on proteins. Influences the hermaphrodite switch from spermatogenesis to oogenesis. Required for body wall muscle cell development. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Developmental protein;Differentiation;Isomerase;Nucleus;Reference proteome;Rotamase;Sexual differentiation;Transferase;Ubl conjugation pathway SUBCELLULAR LOCATION: Nucleus " P52101,PROTEIN NAMES: Sensor histidine kinase GlrK ORGANISM: Escherichia coli (strain K12) FUNCTION: Member of the two-component regulatory system GlrR/GlrK that up-regulates transcription of the glmY sRNA when cells enter the stationary growth phase. Activates GlrR by phosphorylation. KEYWORDS: ATP-binding;Cell inner membrane;Cell membrane;Kinase;Membrane;Nucleotide-binding;Phosphoprotein;Reference proteome;Transferase;Transmembrane;Transmembrane helix;Two-component regulatory system SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein. MISCELLANEOUS: Not required for the regulation of the glmY-glmZ-glmS regulatory cascade by glucosamine-6-phosphate depletion. P52126,"PROTEIN NAMES: Anti-bacteriophage protein B (Probable helicase AbpB) PROTEIN FAMILY: Helicase family ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the helicase family. FUNCTION: Part of an antiviral system composed of AbpA and AbpB; when both are expressed from a plasmid they confer resistance to phages T2, T4, T7 and lambda but not RB32 or RB69. Resistance is temperature dependent, it can be seen at 30 degrees Celsius but not at 37 or 42 degrees Celsius. The system impairs phage but not bacterial DNA synthesis (shown for T4, T7 and lambda). Partially suppressed by mutations in T4 gene 41, a replicative helicase.; FUNCTION: Deletion or mutations in this gene were selected in directed evolution experiments for resistance to intense ionizing radiation (3000 Gy). KEYWORDS: Antiviral defense;ATP-binding;Helicase;Hydrolase;Nucleotide-binding;Reference proteome;Stress response MISCELLANEOUS: Part of prophage CP4-57." P52191,"PROTEIN NAMES: Inward rectifier potassium channel 16 (BIR9) (Inward rectifier K(+) channel Kir5.1) (Potassium channel, inwardly rectifying subfamily J member 16) PROTEIN FAMILY: Inward rectifier-type potassium channel family, KCNJ16 subfamily ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ16 subfamily. FUNCTION: Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. KCNJ16 may be involved in the regulation of fluid and pH balance (By similarity). In the kidney, together with KCNJ10, mediates basolateral K(+) recycling in distal tubules; this process is critical for Na(+) reabsorption at the tubules. KEYWORDS: Cell membrane;Ion channel;Ion transport;Membrane;Phosphoprotein;Potassium;Potassium transport;Reference proteome;Transmembrane;Transmembrane helix;Transport;Voltage-gated channel SUBCELLULAR LOCATION: Membrane ; Multi-pass membrane protein Basolateral cell membrane Note=In kidney distal convoluted tubules, located in the basolateral membrane in the presence of KCNJ10. " P52429,"PROTEIN NAMES: Diacylglycerol kinase epsilon (DAG kinase epsilon) (Diglyceride kinase epsilon) (DGK-epsilon) PROTEIN FAMILY: Eukaryotic diacylglycerol kinase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the eukaryotic diacylglycerol kinase family. FUNCTION: Membrane-bound diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids. Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes. Also plays an important role in the biosynthesis of complex lipids. Displays specificity for diacylglycerol substrates with an arachidonoyl acyl chain at the sn-2 position, with the highest activity toward 1-octadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycerol the main diacylglycerol intermediate within the phosphatidylinositol turnover cycle. Can also phosphorylate diacylglycerol substrates with a linoleoyl acyl chain at the sn-2 position but much less efficiently. PATHWAY: Lipid metabolism; glycerolipid metabolism. KEYWORDS: Alternative splicing;ATP-binding;Cytoplasm;Disease variant;Hemolytic uremic syndrome;Kinase;Lipid metabolism;Membrane;Metal-binding;Nucleotide-binding;Reference proteome;Repeat;Transferase;Transmembrane;Transmembrane helix;Zinc;Zinc-finger SUBCELLULAR LOCATION: Membrane ; Single-pass membrane protein Cytoplasm " P52491,"PROTEIN NAMES: NEDD8-conjugating enzyme UBC12 (RUB1-conjugating enzyme) (Ubiquitin carrier protein 12) PROTEIN FAMILY: Ubiquitin-conjugating enzyme family, UBC12 subfamily ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the ubiquitin-conjugating enzyme family. UBC12 subfamily. FUNCTION: Accepts the ubiquitin-like protein NEDD8/RUB1 from the UBA3-ULA1 E1 complex and catalyzes its covalent attachment to other proteins. The major substrate is CDC53/Cullin. PATHWAY: Protein modification; protein neddylation. KEYWORDS: 3D-structure;Acetylation;ATP-binding;Nucleotide-binding;Reference proteome;Transferase;Ubl conjugation pathway " P52654,PROTEIN NAMES: Transcription initiation factor IIA subunit 1 (General transcription factor IIA subunit 1) (dTFIIA-L) [Cleaved into: Transcription initiation factor IIA alpha chain (TFIIA p30 subunit); Transcription initiation factor IIA beta chain (TFIIA p20 subunit)] PROTEIN FAMILY: TFIIA subunit 1 family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the TFIIA subunit 1 family. FUNCTION: TFIIA is a component of the transcription machinery of RNA polymerase II and plays an important role in transcriptional activation. TFIIA in a complex with TBP mediates transcriptional activity. KEYWORDS: Alternative splicing;Direct protein sequencing;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus. P52687,"PROTEIN NAMES: Sensor histidine kinase CitA ORGANISM: Klebsiella pneumoniae FUNCTION: Member of the two-component regulatory system CitA/CitB. Probably activates CitB by phosphorylation. The periplasmic domain binds H-citrate(2-), which is essential for induction of the citrate-fermentation genes. KEYWORDS: 3D-structure;ATP-binding;Cell inner membrane;Cell membrane;Kinase;Membrane;Nucleotide-binding;Phosphoprotein;Transferase;Transmembrane;Transmembrane helix;Two-component regulatory system SUBCELLULAR LOCATION: Cell inner membrane ; Multi-pass membrane protein " P52699,"PROTEIN NAMES: Metallo-beta-lactamase type 2 (B2 metallo-beta-lactamase) (BLA-IMP) (IMP-1) (Beta-lactamase type II) (Metallo-beta-lactamase type II) PROTEIN FAMILY: Metallo-beta-lactamase superfamily, Class-B beta-lactamase family ORGANISM: Serratia marcescens SIMILARITY: Belongs to the metallo-beta-lactamase superfamily. Class-B beta-lactamase family. FUNCTION: Confers resistance to the different beta-lactams antibiotics (penicillin, cephalosporin and carbapenem) via the hydrolysis of the beta-lactam ring. KEYWORDS: 3D-structure;Antibiotic resistance;Direct protein sequencing;Hydrolase;Metal-binding;Periplasm;Signal;Zinc SUBCELLULAR LOCATION: Periplasm " P52738,PROTEIN NAMES: Zinc finger protein 140 PROTEIN FAMILY: Krueppel C2H2-type zinc-finger protein family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein family. FUNCTION: May be involved in transcriptional regulation as a repressor. KEYWORDS: Alternative splicing;DNA-binding;Metal-binding;Nucleus;Reference proteome;Repeat;Repressor;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus P52739,PROTEIN NAMES: Zinc finger protein 131 PROTEIN FAMILY: Krueppel C2H2-type zinc-finger protein family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein family. FUNCTION: Plays a role during development and organogenesis as well as in the function of the adult central nervous system (By similarity). May be involved in transcriptional regulation as a repressor of ESR1/ER-alpha signaling. KEYWORDS: Alternative splicing;DNA-binding;Isopeptide bond;Metal-binding;Nucleus;Reference proteome;Repeat;Repressor;Transcription;Transcription regulation;Ubl conjugation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Note=Sumoylation does not affect nuclear localization. P52824,"PROTEIN NAMES: Diacylglycerol kinase theta (DAG kinase theta) (DGKtheta) (Diglyceride kinase theta) (DGK-theta) PROTEIN FAMILY: Eukaryotic diacylglycerol kinase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the eukaryotic diacylglycerol kinase family. FUNCTION: Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids. Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes. Within the adrenocorticotropic hormone signaling pathway, produces phosphatidic acid which in turn activates NR5A1 and subsequent steroidogenic gene transcription. Also functions downstream of the nerve growth factor signaling pathway being specifically activated in the nucleus by the growth factor (By similarity). Through its diacylglycerol activity also regulates synaptic vesicle endocytosis. PATHWAY: Lipid metabolism; glycerolipid metabolism. KEYWORDS: ATP-binding;Cell membrane;Cytoplasm;Cytoskeleton;Kinase;Lipid metabolism;Membrane;Metal-binding;Nucleotide-binding;Nucleus;Phosphoprotein;Reference proteome;Repeat;Synapse;Transferase;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cytoplasm Cytoplasm, cytosol Cell membrane Synapse Cytoplasm, cytoskeleton Nucleus Nucleus speckle Nucleus matrix Note=Translocates to the plasma membrane in response to steroid hormone receptor stimulation. Translocation to the plasma membrane is dependent on G-protein coupled receptor stimulation and subsequent activation of PRKCE and probably PRKCH. Translocates to the nucleus in response to thrombin stimulation (Probable). Association with the nuclear matrix is regulated by nerve growth factor (By similarity). " P52891,"PROTEIN NAMES: Nucleoporin NUP84 (Nuclear pore protein NUP84) PROTEIN FAMILY: Nup84/Nup107 nucleoporin family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the Nup84/Nup107 nucleoporin family. FUNCTION: Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. NUP84 is involved in nuclear poly(A)+ RNA export, in NPC assembly and distribution, as well as in nuclear envelope organization. KEYWORDS: 3D-structure;Coiled coil;Membrane;mRNA transport;Nuclear pore complex;Nucleus;Protein transport;Reference proteome;Translocation;Transport SUBCELLULAR LOCATION: Nucleus, nuclear pore complex Nucleus membrane; Peripheral membrane protein; Cytoplasmic side. Nucleus membrane; Peripheral membrane protein; Nucleoplasmic side. Note=Symmetric distribution. MISCELLANEOUS: Present with 8580 molecules/cell in log phase SD medium." P52952,"PROTEIN NAMES: Homeobox protein Nkx-2.5 (Cardiac-specific homeobox) (Homeobox protein CSX) (Homeobox protein NK-2 homolog E) PROTEIN FAMILY: NK-2 homeobox family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the NK-2 homeobox family. FUNCTION: Transcription factor required for the development of the heart and the spleen. During heart development, acts as a transcriptional activator of NPPA/ANF in cooperation with GATA4 (By similarity). May cooperate with TBX2 to negatively modulate expression of NPPA/ANF in the atrioventricular canal (By similarity). Binds to the core DNA motif of NPPA promoter. Together with PBX1, required for spleen development through a mechanism that involves CDKN2B repression. Positively regulates transcription of genes such as COL3A1 and MMP2, resulting in increased pulmonary endothelial fibrosis in response to hypoxia. KEYWORDS: 3D-structure;Alternative splicing;Atrial septal defect;Congenital hypothyroidism;Developmental protein;Disease variant;DNA-binding;Homeobox;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus " P53032,"PROTEIN NAMES: Sterol uptake protein 1 PROTEIN FAMILY: SUT1 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the SUT1 family. FUNCTION: Transcription factor involved in the induction of hypoxic gene transcription when the cells are shifted from aerobiosis to anaerobiosis. Promotes exogenous sterol uptake and stimulates endogenous sterol biosynthesis. With SUT2, positively regulates mating by repressing the expression of the mating inhibitors NCE102, PRR2 and RHO5 in response to pheromone. Finally, regulates filamentation via the repression of genes such as GAT2, HAP4, MGA1, MSN4, NCE102, PRR2, RHO3, and RHO5, when nutrients are plentifu. KEYWORDS: Cytoplasm;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Cytoplasm Nucleus MISCELLANEOUS: Present with 1100 molecules/cell in log phase SD medium." P53038,"PROTEIN NAMES: Telomere length regulation protein TEL2 PROTEIN FAMILY: TEL2 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the TEL2 family. FUNCTION: Part of the TTT complex that is required to stabilize protein levels of the phosphatidylinositol 3-kinase-related protein kinase (PIKK) family proteins (By similarity). Required for telomere length regulation and telomere position effect. Regulates telomere length and participates in gene silencing at subtelomeric regions. Binds to telomeric DNA repeats. KEYWORDS: 3D-structure;Chromosome;DNA-binding;Nucleus;Phosphoprotein;Reference proteome;Telomere SUBCELLULAR LOCATION: Nucleus Chromosome, telomere MISCELLANEOUS: Present with 638 molecules/cell in log phase SD medium." P53043,"PROTEIN NAMES: Serine/threonine-protein phosphatase T (PPT) PROTEIN FAMILY: PPP phosphatase family, PP-5 (PP-T) subfamily ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the PPP phosphatase family. PP-5 (PP-T) subfamily. FUNCTION: Protein phosphatase that specifically binds to and dephosphorylates the molecular chaperone Hsp90 (HSC82 and HSP82). Dephosphorylation positively regulates the Hsp90 chaperone machinery. KEYWORDS: 3D-structure;Hydrolase;Manganese;Metal-binding;Nucleus;Protein phosphatase;Reference proteome;Repeat;TPR repeat SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: Present with 6990 molecules/cell in log phase SD medium." P53045,"PROTEIN NAMES: C-4 methylsterol oxidase ERG25 (Ergosterol biosynthetic protein 25) (Sterol-C4-methyl oxidase ERG25) (SMO) PROTEIN FAMILY: Sterol desaturase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the sterol desaturase family. FUNCTION: C-4 methylsterol oxidase; part of the third module of ergosterol biosynthesis pathway that includes the late steps of the pathway. ERG25 is a catalytic component of the C-4 demethylation complex that catalyzes the three-step monooxygenation required for the demethylation of 4,4-dimethyl and 4alpha-methylsterols. The third module or late pathway involves the ergosterol synthesis itself through consecutive reactions that mainly occur in the endoplasmic reticulum (ER) membrane. Firstly, the squalene synthase ERG9 catalyzes the condensation of 2 farnesyl pyrophosphate moieties to form squalene, which is the precursor of all steroids. Squalene synthase is crucial for balancing the incorporation of farnesyl diphosphate (FPP) into sterol and nonsterol isoprene synthesis. Secondly, the squalene epoxidase ERG1 catalyzes the stereospecific oxidation of squalene to (S)-2,3-epoxysqualene, which is considered to be a rate-limiting enzyme in steroid biosynthesis. Then, the lanosterol synthase ERG7 catalyzes the cyclization of (S)-2,3 oxidosqualene to lanosterol, a reaction that forms the sterol core. In the next steps, lanosterol is transformed to zymosterol through a complex process involving various demethylation, reduction and desaturation reactions. The lanosterol 14-alpha-demethylase ERG11 (also known as CYP51) catalyzes C14-demethylation of lanosterol to produce 4,4'-dimethyl cholesta-8,14,24-triene-3-beta-ol, which is critical for ergosterol biosynthesis. The C-14 reductase ERG24 reduces the C14=C15 double bond of 4,4-dimethyl-cholesta-8,14,24-trienol to produce 4,4-dimethyl-cholesta-8,24-dienol. 4,4-dimethyl-cholesta-8,24-dienol is substrate of the C-4 demethylation complex ERG25-ERG26-ERG27 in which ERG25 catalyzes the three-step monooxygenation required for the demethylation of 4,4-dimethyl and 4alpha-methylsterols, ERG26 catalyzes the oxidative decarboxylation that results in a reduction of the 3-beta-hydroxy group at the C-3 carbon to an oxo group, and ERG27 is responsible for the reduction of the keto group on the C-3. ERG28 has a role as a scaffold to help anchor ERG25, ERG26 and ERG27 to the endoplasmic reticulum and ERG29 regulates the activity of the iron-containing C4-methylsterol oxidase ERG25. Then, the sterol 24-C-methyltransferase ERG6 catalyzes the methyl transfer from S-adenosyl-methionine to the C-24 of zymosterol to form fecosterol. The C-8 sterol isomerase ERG2 catalyzes the reaction which results in unsaturation at C-7 in the B ring of sterols and thus converts fecosterol to episterol. The sterol-C5-desaturase ERG3 then catalyzes the introduction of a C-5 double bond in the B ring to produce 5-dehydroepisterol. The C-22 sterol desaturase ERG5 further converts 5-dehydroepisterol into ergosta-5,7,22,24(28)-tetraen-3beta-ol by forming the C-22(23) double bond in the sterol side chain. Finally, ergosta-5,7,22,24(28)-tetraen-3beta-ol is substrate of the C-24(28) sterol reductase ERG4 to produce ergosterol. PATHWAY: Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 3/6.; PATHWAY: Steroid metabolism; ergosterol biosynthesis. KEYWORDS: Endoplasmic reticulum;Iron;Isopeptide bond;Lipid biosynthesis;Lipid metabolism;Membrane;NAD;Oxidoreductase;Reference proteome;Steroid biosynthesis;Steroid metabolism;Sterol biosynthesis;Sterol metabolism;Transmembrane;Transmembrane helix;Ubl conjugation SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Single-pass membrane protein MISCELLANEOUS: Present with 77100 molecules/cell in log phase SD medium." P53068,"PROTEIN NAMES: Anaphase-promoting complex subunit DOC1 (Destruction of cyclin B protein 1) PROTEIN FAMILY: APC10 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the APC10 family. FUNCTION: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin-protein ligase complex that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C is thought to confer substrate specificity and, in the presence of ubiquitin-conjugating E2 enzymes, it catalyzes the formation of protein-ubiquitin conjugates that are subsequently degraded by the 26S proteasome. In early mitosis, the APC/C is activated by CDC20 and targets securin PDS1, the B-type cyclin CLB5, and other anaphase inhibitory proteins for proteolysis, thereby triggering the separation of sister chromatids at the metaphase-to-anaphase transition. In late mitosis and in G1, degradation of CLB5 allows activation of the APC/C by CDH1, which is needed to destroy CDC20 and the B-type cyclin CLB2 to allow exit from mitosis and creating the low CDK state necessary for cytokinesis and for reforming prereplicative complexes in G1 prior to another round of replication. DOC1 is required, together with the coactivators CDH1 and CDC20, for recognition and binding of the substrates. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: 3D-structure;Cell cycle;Cell division;Cytoplasm;Mitosis;Nucleus;Reference proteome;Ubl conjugation pathway SUBCELLULAR LOCATION: Cytoplasm Nucleus MISCELLANEOUS: Present with 1364 molecules/cell in log phase SD medium." P53079,"PROTEIN NAMES: Conserved oligomeric Golgi complex subunit 1 (COG complex subunit 1) (Complexed with DOR1 protein 3) (Component of oligomeric Golgi complex 1) (Protein SEC36) PROTEIN FAMILY: COG1 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the COG1 family. FUNCTION: Acts as essential component of the peripheral membrane COG complex that is involved in intra-Golgi protein trafficking. COG is located at the cis-Golgi, and regulates tethering of retrograde intra-Golgi vesicles and possibly a number of other membrane trafficking events. KEYWORDS: Acetylation;Golgi apparatus;Membrane;Phosphoprotein;Protein transport;Reference proteome;Transport SUBCELLULAR LOCATION: Golgi apparatus membrane ; Peripheral membrane protein ; Cytoplasmic side MISCELLANEOUS: Present with 2190 molecules/cell in log phase SD medium." P53086,"PROTEIN NAMES: Kinesin-like protein KIP3 PROTEIN FAMILY: TRAFAC class myosin-kinesin ATPase superfamily, Kinesin family, Kinesin II subfamily ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. Kinesin II subfamily. KEYWORDS: ATP-binding;Coiled coil;Cytoplasm;Cytoskeleton;Microtubule;Motor protein;Nucleotide-binding;Reference proteome SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton Note=Colocalizes with beta-tubulin TUB2 at the plus ends of growing microtubules and along the microtubule lattice. MISCELLANEOUS: Present with 736 molecules/cell in log phase SD medium." P53090,"PROTEIN NAMES: Aromatic/aminoadipate aminotransferase 1 (2-aminoadipate aminotransferase) (2-aminoadipate transaminase) (Alpha-aminoadipate aminotransferase) (AadAT) (Aromatic amino acid aminotransferase 1) (Aromatic amino acid aminotransferase I) (Aromatic amino acid-requiring protein 8) PROTEIN FAMILY: Class-I pyridoxal-phosphate-dependent aminotransferase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. FUNCTION: General aromatic amino acid transaminase involved in several otherwise unrelated metabolic pathways. Responsible for phenylalanine and tyrosine biosynthesis. Active with glutamate, phenylalanine, tyrosine and tryptophan as amino donors and with phenylpyruvate, hydroxyphenylpyruvate, 2-oxoglutarate and pyruvate as amino acceptors. Also active with methionine, alpha-aminoadipate and leucine as amino donors when phenylpyruvate is the amino acceptor and in the reverse reactions with the corresponding oxo acids and phenylalanine as amino donor. Catalyzes the formation of methionine from 2-keto-4-methylthiobutyrate (KMTB) in the methionine salvage pathway primarily using aromatic amino acids (tyrosine, phenylalanine and tryptophan) as the amino donors. Catalyzes the formation of alpha-aminoadipate from alpha-ketoadipate in the lysine biosyntheic pathway. PATHWAY: Amino-acid biosynthesis; L-phenylalanine biosynthesis; L-phenylalanine from phenylpyruvate (ArAT route): step 1/1.; PATHWAY: Amino-acid biosynthesis; L-tyrosine biosynthesis; L-tyrosine from (4-hydroxyphenyl)pyruvate: step 1/1.; PATHWAY: Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 6/6.; PATHWAY: Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-alpha-aminoadipate from 2-oxoglutarate: step 5/5. KEYWORDS: 3D-structure;Aminotransferase;Cytoplasm;Phosphoprotein;Pyridoxal phosphate;Reference proteome;Transferase SUBCELLULAR LOCATION: Cytoplasm MISCELLANEOUS: Present with 1770 molecules/cell in log phase SD medium." P53095,PROTEIN NAMES: D-serine dehydratase (D-serine deaminase) (DSD) PROTEIN FAMILY: DSD1 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the DSD1 family. FUNCTION: Catalyzes the conversion of D-serine to pyruvate and ammonia. May play a role in D-serine detoxification. KEYWORDS: Detoxification;Lyase;Metal-binding;Pyridoxal phosphate;Reference proteome;Zinc P53129,"PROTEIN NAMES: Vacuolar fusion protein MON1 (Autophagy-related protein 12) (Monensin sensitivity protein 1) PROTEIN FAMILY: MON1/SAND family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the MON1/SAND family. FUNCTION: In complex with CCZ1, is required for multiple vacuole delivery pathways including the cytoplasm to vacuole transport (Cvt), autophagy, pexophagy and endocytosis. The MON1-CCZ1 complex acts at the fusion of vesicles with the vacuole, through its regulation of the SNARE complex during the coordinated priming and docking stages of fusion, and particularly at the stage of tethering/docking. The MON1-CCZ1 complex is recruited to membranes enriched in charged lipids, particularly phosphatidylinositol 3-phosphate (PtdIns[3]P), by GTP-associated small GTPase RAB5 homologs (YPT10, YPT52, YPT53 and VPS21). The MON1-CCZ1 complex recruits GDP-associated small GTPase YPT7 to membranes and acts as a guanine nucleotide-exchange factor (GEF), promoting nucleotide-exchange on YPT7 and triggering endosomal maturation by recruiting downstream effectors such as components of the HOPS tethering complex. KEYWORDS: Autophagy;Endosome;Membrane;Phosphoprotein;Protein transport;Reference proteome;Transport;Vacuole SUBCELLULAR LOCATION: Endosome, multivesicular body membrane ; Peripheral membrane protein. Prevacuolar compartment membrane ; Peripheral membrane protein. Vacuole membrane ; Peripheral membrane protein. Note=The association of the MON1-CCZ1 complex with the vacuole is regulated by the C-Vps/HOPS complex. " P53137,"PROTEIN NAMES: RQC trigger complex subunit CUE3 (CUE domain-containing protein 3) (Coupling of ubiquitin conjugation to ER degradation protein 3) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Involved in activation of the ribosome quality control (RQC) pathway, a pathway that degrades nascent peptide chains during problematic translation. Specifically recognizes and binds RPS20/uS10 ubiquitinated by HEL2, promoting recruitment of the RQT (ribosome quality control trigger) complex on stalled ribosomes, followed by disassembly of stalled ribosomes. KEYWORDS: 3D-structure;Cytoplasm;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cytoplasm MISCELLANEOUS: Present with 6300 molecules/cell in log phase SD medium." P53140,"PROTEIN NAMES: RNA-binding protein RMD9, mitochondrial (Dodecamer binding protein) (DBP) (Required for meiotic nuclear division protein 9) PROTEIN FAMILY: RMD9 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the RMD9 family. FUNCTION: Binds the RNA motif 5'-AAUAA[U/C]AUUCUU-3' in the 3'-UTR of mitochondrial mRNAs. Involved in the processing or stability of mitochondrial mRNAs. KEYWORDS: 3D-structure;Direct protein sequencing;Membrane;Mitochondrion;Mitochondrion inner membrane;Phosphoprotein;Reference proteome;Repeat;RNA-binding;Sporulation;Transit peptide SUBCELLULAR LOCATION: Mitochondrion inner membrane ; Peripheral membrane protein ; Matrix side MISCELLANEOUS: Present with 4800 molecules/cell in log phase SD medium." P53145,"PROTEIN NAMES: Large subunit GTPase 1 PROTEIN FAMILY: TRAFAC class YlqF/YawG GTPase family, LSG1 subfamily ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the TRAFAC class YlqF/YawG GTPase family. LSG1 subfamily. FUNCTION: GTPase required for the nuclear export of the 60S ribosomal subunit. Acts by mediating the release of NMD3 from the 60S ribosomal subunit after export into the cytoplasm. KEYWORDS: 3D-structure;Cytoplasm;GTP-binding;Hydrolase;Nucleotide-binding;Phosphoprotein;Protein transport;Reference proteome;Transport SUBCELLULAR LOCATION: Cytoplasm MISCELLANEOUS: Present with 19400 molecules/cell in log phase SD medium." P53150,"PROTEIN NAMES: Ligase-interacting factor 1 PROTEIN FAMILY: XRCC4-XLF family, XLF subfamily ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the XRCC4-XLF family. XLF subfamily. FUNCTION: Stabilizes DNL4. Involved in non-homologous repair of DNA double-strand breaks. KEYWORDS: 3D-structure;Cytoplasm;DNA damage;DNA repair;Nucleus;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Nucleus MISCELLANEOUS: Present with 876 molecules/cell in log phase SD medium." P53170,"PROTEIN NAMES: [Pyruvate dehydrogenase (acetyl-transferring)] kinase 2, mitochondrial (PDK 2) (Pyruvate dehydrogenase kinase 2) (Protein kinase of PDH protein 2) (Pyruvate dehydrogenase complex kinase 2) (PDC kinase 2) ([Pyruvate dehydrogenase [lipoamide]] kinase 2) PROTEIN FAMILY: PDK/BCKDK protein kinase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the PDK/BCKDK protein kinase family. FUNCTION: Inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit (PDA1), thus contributing to the regulation of glucose metabolism. KEYWORDS: ATP-binding;Kinase;Mitochondrion;Nucleotide-binding;Reference proteome;Transferase;Transit peptide SUBCELLULAR LOCATION: Mitochondrion matrix MISCELLANEOUS: Present with 450 molecules/cell in log phase SD medium." P53178,PROTEIN NAMES: UDP-N-acetylglucosamine transferase subunit ALG13 (Asparagine-linked glycosylation protein 13) PROTEIN FAMILY: Glycosyltransferase 28 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the glycosyltransferase 28 family. FUNCTION: Involved in protein N-glycosylation. Essential for the second step of the dolichol-linked oligosaccharide pathway. KEYWORDS: 3D-structure;Endoplasmic reticulum;Glycosyltransferase;Reference proteome;Transferase SUBCELLULAR LOCATION: Endoplasmic reticulum MISCELLANEOUS: Present with 1950 molecules/cell in log phase SD medium. P53184,"PROTEIN NAMES: Nicotinamidase (Nicotinamide deamidase) (NAMase) PROTEIN FAMILY: Isochorismatase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the isochorismatase family. FUNCTION: Catalyzes the deamidation of nicotinamide, an early step in the NAD(+) salvage pathway. Positively regulates SIR2-mediated silencing and longevity by preventing the accumulation of intracellular nicotinamide, an inhibitor of SIR2, during times of stress. Acts also on nicotinyl hydroxamate. PATHWAY: Cofactor biosynthesis; nicotinate biosynthesis; nicotinate from nicotinamide: step 1/1. KEYWORDS: 3D-structure;Cytoplasm;Direct protein sequencing;Hydrolase;Metal-binding;Nucleus;Peroxisome;Pyridine nucleotide biosynthesis;Reference proteome;Zinc SUBCELLULAR LOCATION: Cytoplasm Nucleus Peroxisome Note=Concentrates in peroxisomes. MISCELLANEOUS: Has a cis-peptide bond at 162-Val-Ala-163.; MISCELLANEOUS: Present with 7720 molecules/cell in log phase SD medium." P53191,"PROTEIN NAMES: Glutamine sensor PIB2 (Phosphatidylinositol 3-phosphate-binding protein 2) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Functions as an intracellular glutamine sensor that directly activates the TORC1 signaling pathway, to promote cell growth when glutamine is available. May play a role in repressing NPR1 activity independently of TORC1 signaling. KEYWORDS: Membrane;Metal-binding;Phosphoprotein;Reference proteome;Transducer;Vacuole;Zinc;Zinc-finger SUBCELLULAR LOCATION: Vacuole membrane ; Peripheral membrane protein " P53195,"PROTEIN NAMES: Conserved oligomeric Golgi complex subunit 7 (COG complex subunit 7) (Complexed with DOR1 protein 5) (Component of oligomeric Golgi complex 7) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Acts as a component of the peripheral membrane COG complex that is involved in intra-Golgi protein trafficking. COG is located at the cis-Golgi, and regulates tethering of retrograde intra-Golgi vesicles and possibly a number of other membrane trafficking events. KEYWORDS: Golgi apparatus;Membrane;Protein transport;Reference proteome;Transport SUBCELLULAR LOCATION: Golgi apparatus membrane ; Peripheral membrane protein MISCELLANEOUS: Present with 1360 molecules/cell in log phase SD medium." P53197,"PROTEIN NAMES: APC/C activator protein CDH1 (CDC20 homolog 1) (Homolog of CDC twenty 1) PROTEIN FAMILY: WD repeat CDC20/Fizzy family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the WD repeat CDC20/Fizzy family. FUNCTION: Activator protein that regulates the ubiquitin ligase activity and substrate specificity of the anaphase promoting complex/cyclosome (APC/C). During telophase and in the subsequent G1 phase of the cell cycle, recognizes and binds proteins containing a destruction box (D-box) and an additional degradation signal termed the KEN box including ASE1, CDC20, the B-type cyclins CLB2 and CLB3, the polo-like kinase CDC5 and HSL1, and recruits them in a C-box-dependent manner to the APC/C for ubiquitination and subsequent proteolysis. Required for exit from mitosis, cytokinesis and formation of prereplicative complexes in G1. Probably is the target of a BUB2-dependent spindle checkpoint pathway. KEYWORDS: 3D-structure;Cell cycle;Cell division;Cytoplasm;Mitosis;Nucleus;Phosphoprotein;Reference proteome;Repeat;WD repeat SUBCELLULAR LOCATION: Cytoplasm Nucleus Note=Nuclear import and export are mediated by the importin PSE1 and the exportin MSN5. " P53230,"PROTEIN NAMES: Phosphatidate cytidylyltransferase, mitochondrial (CDP-diacylglycerol synthase) (CDP-DAG synthase) (Mitochondrial import protein MMP37) (Mitochondrial matrix protein of 37 kDa) (Mitochondrial translocator assembly and maintenance protein 41) PROTEIN FAMILY: TAM41 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the TAM41 family. FUNCTION: Catalyzes the formation of CDP-diacylglycerol (CDP-DAG) from phosphatidic acid (PA) in the mitochondrial inner membrane. Required for the biosynthesis of the dimeric phospholipid cardiolipin, which stabilizes supercomplexes of the mitochondrial respiratory chain in the mitochondrial inner membrane. PATHWAY: Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 3/3. KEYWORDS: Lipid biosynthesis;Lipid metabolism;Magnesium;Membrane;Mitochondrion;Mitochondrion inner membrane;Nucleotidyltransferase;Phospholipid biosynthesis;Phospholipid metabolism;Reference proteome;Transferase SUBCELLULAR LOCATION: Mitochondrion inner membrane ; Peripheral membrane protein ; Matrix side MISCELLANEOUS: Present with 195 molecules/cell in log phase SD medium." P53238,"PROTEIN NAMES: Peflin (Penta-EF hand domain-containing protein 1) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Calcium-binding protein that is required for polar bud growth and cell wall abscission. Can also bind zinc ions. KEYWORDS: Calcium;Cytoplasm;Metal-binding;Nucleus;Reference proteome;Repeat SUBCELLULAR LOCATION: Cytoplasm Nucleus Bud tip Bud neck Note=Accumulates at the site of bud emergence in G1 cells. In small budded G1 cells, localizes asymmetrically on the tip of the daughter cell and in the nucleus. At a later stage of the G1 phase, is found at the emerging bud cortex and localizes in the nucleus. In large budded G2/M cells, localizes preferentially at the bud neck between the dividing mother and daughter cells, with very weak signals in the nucleus. MISCELLANEOUS: Present with 1630 molecules/cell in log phase SD medium." P53255,"PROTEIN NAMES: CWF19-like protein DRN1 (Debranching enzyme-associated ribonuclease 1) PROTEIN FAMILY: CWF19 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the CWF19 family. FUNCTION: Involved in branched RNA metabolism, modulating the turnover of lariat-intron pre-mRNAs by the lariat-debranching enzyme DBR1. Enhances the debranching activity of DBR1 in vitro. KEYWORDS: Cytoplasm;mRNA processing;Nucleus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=Relocalizes to the cytoplasm in response to hypoxia. MISCELLANEOUS: Present with 1270 molecules/cell in log phase SD medium." P53256,"PROTEIN NAMES: Exosome complex component RRP46 (Ribosomal RNA-processing protein 46) PROTEIN FAMILY: RNase PH family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the RNase PH family. FUNCTION: Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and cryptic unstable transcripts (CUTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and in RNA surveillance pathways, preventing translation of aberrant mRNAs. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. RRP46 is part of the hexameric ring of RNase PH domain-containing subunits proposed to form a central channel which threads RNA substrates for degradation. KEYWORDS: 3D-structure;Cytoplasm;Exosome;Nucleus;Reference proteome;RNA-binding;rRNA processing SUBCELLULAR LOCATION: Cytoplasm Nucleus, nucleolus MISCELLANEOUS: Present with 10800 molecules/cell in log phase SD medium." P53264,"PROTEIN NAMES: Cardiolipin-specific deacylase 1, mitochondrial PROTEIN FAMILY: Peptidase S33 family, ABHD4/ABHD5 subfamily ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the peptidase S33 family. ABHD4/ABHD5 subfamily. FUNCTION: Mitochondrial cardiolipin-specific phospholipase which deacylates de novo synthesized cardiolipin (CL). Part of the remodeling process of cardiolipin, which involves deacylation-reacylation of premature cardiolipin. Has a strong substrate preference for palmitic acid residues and generates monolysocardiolipin (MLCL) for TAZ1-dependent reacylation with unsaturated fatty acids. The hydrolytic selectivity of the enzyme toward C16-CL substrates contributes to the preservation of C18:1-containing CL species. Has high specificity towards peroxidized cardiolipids CL(OX). Required to mitigate oxidative stress by removing CL(OX). KEYWORDS: Hydrolase;Membrane;Mitochondrion;Mitochondrion inner membrane;Reference proteome;Transferase;Transit peptide SUBCELLULAR LOCATION: Mitochondrion Mitochondrion inner membrane ; Peripheral membrane protein ; Matrix side " P53266,PROTEIN NAMES: Cytochrome oxidase assembly protein SHY1 (SURF1 homolog of Yeast) (SURF1-like protein) PROTEIN FAMILY: SURF1 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the SURF1 family. FUNCTION: Required for efficient assembly of cytochrome c oxidase in the mitochondrial inner membrane. Involved in a step that couples MSS51-COX14-dependent regulation of COX1 translation to early steps of cytochrome c oxidase assembly. KEYWORDS: Membrane;Mitochondrion;Mitochondrion inner membrane;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Mitochondrion inner membrane ; Multi-pass membrane protein MISCELLANEOUS: Present with 623 molecules/cell in log phase SD medium. P53271,"PROTEIN NAMES: Conserved oligomeric Golgi complex subunit 2 (COG complex subunit 2) (Component of oligomeric Golgi complex 2) (Protein SEC35) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Acts as a component of the peripheral membrane COG complex that is involved in intra-Golgi protein trafficking. COG is located at the cis-Golgi, and regulates tethering of retrograde intra-Golgi vesicles and possibly a number of other membrane trafficking events. COG2 is required for ER to Golgi vesicle docking. Not essential for viability. KEYWORDS: 3D-structure;Golgi apparatus;Membrane;Protein transport;Reference proteome;Transport SUBCELLULAR LOCATION: Golgi apparatus membrane ; Peripheral membrane protein ; Cytoplasmic side MISCELLANEOUS: Present with 3270 molecules/cell in log phase SD medium." P53290,"PROTEIN NAMES: GTP-binding protein GTR2 PROTEIN FAMILY: GTR/RAG GTP-binding protein family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the GTR/RAG GTP-binding protein family. FUNCTION: GTPase involved in activation of the TORC1 signaling pathway, which promotes growth and represses autophagy in nutrient-rich conditions. Also required for TORC1 inactivation during nitrogen starvation. Required for intracellular sorting of GAP1 out of the endosome. Involved in the regulation of microautophagy. KEYWORDS: 3D-structure;GTP-binding;Hydrolase;Membrane;Nucleotide-binding;Reference proteome;Vacuole SUBCELLULAR LOCATION: Vacuole membrane ; Peripheral membrane protein MISCELLANEOUS: Present with 2610 molecules/cell in log phase SD medium." P53298,"PROTEIN NAMES: Inner kinetochore subunit OKP1 (CENP-Q homolog) (Constitutive centromere-associated network protein OKP1) (Outer kinetochore protein 1) PROTEIN FAMILY: CENP-Q/OKP1 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the CENP-Q/OKP1 family. FUNCTION: Component of the kinetochore, a multiprotein complex that assembles on centromeric DNA and attaches chromosomes to spindle microtubules, mediating chromosome segregation and sister chromatid segregation during meiosis and mitosis. Component of the inner kinetochore COMA complex, which connects centromere-associated proteins and the outer kinetochore. COMA interacts with other inner kinetochore proteins to form the inner kinetochore constitutive centromere-associated network (CCAN), which serves as a structural platform for outer kinetochore assembly. KEYWORDS: 3D-structure;Cell cycle;Cell division;Centromere;Chromosome;Coiled coil;Kinetochore;Meiosis;Mitosis;Nucleus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Nucleus Chromosome, centromere, kinetochore MISCELLANEOUS: Present with 2688 molecules/cell in log phase SD medium." P53303,PROTEIN NAMES: Zinc finger chaperone ZPR1 PROTEIN FAMILY: ZPR1 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the ZPR1 family. FUNCTION: Acts as a protein folding chaperone for elongation factor 1-alpha. KEYWORDS: Chaperone;Cytoplasm;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Repeat;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cytoplasm Nucleus Note=Translocates to the nucleus after nutrient stimulation. MISCELLANEOUS: Present with 39900 molecules/cell in log phase SD medium. P53312,"PROTEIN NAMES: Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial (Succinyl-CoA synthetase beta chain) (SCS-beta) PROTEIN FAMILY: Succinate/malate CoA ligase beta subunit family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the succinate/malate CoA ligase beta subunit family. FUNCTION: Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of ATP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit (By similarity). PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1. KEYWORDS: ATP-binding;Ligase;Magnesium;Metal-binding;Mitochondrion;Nucleotide-binding;Phosphoprotein;Reference proteome;Transit peptide;Tricarboxylic acid cycle SUBCELLULAR LOCATION: Mitochondrion " P53324,PROTEIN NAMES: Steryl acetyl hydrolase 1 PROTEIN FAMILY: 'GDXG' lipolytic enzyme family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the 'GDXG' lipolytic enzyme family. FUNCTION: Required for the deacetylation of acetylated sterols. Involved in the resistance to eugenol and pregnenolone toxicity. KEYWORDS: Acetylation;Endoplasmic reticulum;Glycoprotein;Hydrolase;Membrane;Reference proteome;Signal-anchor;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Single-pass type II membrane protein P53347,"PROTEIN NAMES: Oncostatin-M (OSM) PROTEIN FAMILY: LIF/OSM family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the LIF/OSM family. FUNCTION: Growth regulator. Inhibits the proliferation of a number of tumor cell lines. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells (By similarity). Uses only type II OSM receptor (heterodimers composed of OSMR and IL6ST). Involved in the maturation of fetal hepatocytes, thereby promoting liver development and regeneration (By similarity). KEYWORDS: Cleavage on pair of basic residues;Cytokine;Disulfide bond;Glycoprotein;Growth regulation;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted. " P53367,"PROTEIN NAMES: Arfaptin-1 (ADP-ribosylation factor-interacting protein 1) ORGANISM: Homo sapiens (Human) FUNCTION: Plays a role in controlling biogenesis of secretory granules at the trans-Golgi network. Mechanistically, binds ARF-GTP at the neck of a growing secretory granule precursor and forms a protective scaffold. Once the granule precursor has been completely loaded, active PRKD1 phosphorylates ARFIP1 and releases it from ARFs. In turn, ARFs induce fission. Through this mechanism, ensures proper secretory granule formation at the Golgi of pancreatic beta cells. KEYWORDS: Acetylation;Alternative splicing;Golgi apparatus;Membrane;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Golgi apparatus Golgi apparatus, trans-Golgi network membrane " P53378,"PROTEIN NAMES: Tubulin gamma chain (Gamma-tubulin) PROTEIN FAMILY: Tubulin family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the tubulin family. FUNCTION: Tubulin is the major constituent of microtubules. The gamma chain is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome, suggesting that it is involved in the minus-end nucleation of microtubule assembly. TUB4 is an important spindle pole body component that organizes both cytoplasmic and nuclear microtubule arrays. KEYWORDS: 3D-structure;Cytoplasm;Cytoskeleton;GTP-binding;Microtubule;Nucleotide-binding;Reference proteome SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body. MISCELLANEOUS: Present with 7200 molecules/cell in log phase SD medium." P53379,"PROTEIN NAMES: Aspartic proteinase MKC7 (Yapsin-2) PROTEIN FAMILY: Peptidase A1 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the peptidase A1 family. FUNCTION: Cleaves proteins C-terminally to the most C-terminal basic residue. Can process the alpha-mating factor precursor. Required for cell wall integrity. KEYWORDS: Aspartyl protease;Cell membrane;Direct protein sequencing;Glycoprotein;GPI-anchor;Hydrolase;Lipoprotein;Membrane;Protease;Reference proteome;Signal;Zymogen SUBCELLULAR LOCATION: Cell membrane ; Lipid-anchor, GPI-anchor Note=GPI-anchored plasma membrane protein (GPI-PMP). MISCELLANEOUS: Present with 538 molecules/cell in log phase SD medium." P53540,"PROTEIN NAMES: Spindle pole body component SPC98 PROTEIN FAMILY: TUBGCP family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the TUBGCP family. FUNCTION: Involved in microtubule organization by the microtubule organizing center, the spindle pole body (SPB). Probably part of the microtubule attachment site at the SPB. KEYWORDS: 3D-structure;Cytoplasm;Cytoskeleton;Microtubule;Nucleus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body. MISCELLANEOUS: Present with 56 molecules/cell in log phase SD medium." P53541,"PROTEIN NAMES: Putative meiotic phospholipase SPO1 (Sporulation-specific protein 1) PROTEIN FAMILY: Lysophospholipase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the lysophospholipase family. FUNCTION: Regulates spindle pole duplication in meiosis I, but not in mitosis. Required for meiosis I, meiosis II chromosome segregation and spore formation. Binds phosphatidylinositol (4)P mono- and polyphosphates. KEYWORDS: Endoplasmic reticulum;Glycoprotein;Hydrolase;Lipid degradation;Lipid metabolism;Meiosis;Membrane;Nucleus;Reference proteome;Signal;Sporulation;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein. " P53554,PROTEIN NAMES: Biotin biosynthesis cytochrome P450 PROTEIN FAMILY: Cytochrome P450 family ORGANISM: Bacillus subtilis (strain 168) SIMILARITY: Belongs to the cytochrome P450 family. FUNCTION: Catalyzes the C-C bond cleavage of fatty acid linked to acyl carrier protein (ACP) to generate pimelic acid for biotin biosynthesis. It has high affinity for long-chain fatty acids with the greatest affinity for myristic acid. PATHWAY: Cofactor biosynthesis; biotin biosynthesis. KEYWORDS: 3D-structure;Biotin biosynthesis;Direct protein sequencing;Disulfide bond;Heme;Iron;Metal-binding;Monooxygenase;Oxidoreductase;Reference proteome P53617,PROTEIN NAMES: Protein NRD1 ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Plays a role in sequence-specific regulation of nuclear pre-mRNA abundance. KEYWORDS: 3D-structure;Nucleus;Phosphoprotein;Reference proteome;RNA-binding SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: Present with 19600 molecules/cell in log phase SD medium. P53687,"PROTEIN NAMES: NAD-dependent histone deacetylase HST3 (Homologous to SIR2 protein 3) (Regulatory protein SIR2 homolog 3) PROTEIN FAMILY: Sirtuin family, Class I subfamily ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the sirtuin family. Class I subfamily. FUNCTION: NAD-dependent histone deacetylase, which contributes together with HST4 to histone H3 'Lys-56' deacetylation, regulation of telomeric silencing, proper cell cycle progression, DNA damage control, DNA recombination, and genomic maintenance. KEYWORDS: Cytoplasm;Metal-binding;NAD;Nucleus;Reference proteome;Repressor;Transcription;Transcription regulation;Transferase;Zinc SUBCELLULAR LOCATION: Cytoplasm. Nucleus. MISCELLANEOUS: Present with 319 molecules/cell in log phase SD medium." P53688,"PROTEIN NAMES: NAD-dependent histone deacetylase HST4 (Homologous to SIR2 protein 4) (Regulatory protein SIR2 homolog 4) PROTEIN FAMILY: Sirtuin family, Class I subfamily ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the sirtuin family. Class I subfamily. FUNCTION: NAD-dependent histone deacetylase, which contributes together with HST3 to histone H3 'Lys-56' deacetylation, regulation of telomeric silencing, proper cell cycle progression, DNA damage control, DNA recombination, and genomic maintenance. KEYWORDS: Metal-binding;NAD;Nucleus;Reference proteome;Repressor;Transcription;Transcription regulation;Transferase;Zinc SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: Present with 377 molecules/cell in log phase SD medium." P53738,"PROTEIN NAMES: Multifunctional methyltransferase subunit TRM112 (eRF1 methyltransferase subunit TRM112) (eRF1 MTase subunit TRM112) (tRNA methyltransferase 112) PROTEIN FAMILY: TRM112 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the TRM112 family. FUNCTION: Acts as an activator of both rRNA/tRNA and protein methyltransferases. Together with methyltransferase MTQ2, required for the methylation of eRF1 on 'Gln-182'. Together with methyltransferase TRM11, required for the formation of 2-methylguanosine at position 10 (m2G10) in tRNA. Together with methyltransferase BUD23, required for the formation of 7-methylguanine at position 1575 (m7G1575) in 18S rRNA. Involved in biogenesis of both 40S and 60S ribosomal subunits. KEYWORDS: 3D-structure;Cytoplasm;Nucleus;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Nucleus MISCELLANEOUS: Present with 4800 molecules/cell in log phase SD medium." P53838,"PROTEIN NAMES: Boron transporter 1 PROTEIN FAMILY: Anion exchanger family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the anion exchanger (TC 2.A.31) family. FUNCTION: Functions in boric acid/borate export across the plasma membrane, and thereby protects yeast cells from boron toxicity. Involved in the trafficking of proteins to the vacuole. KEYWORDS: Anion exchange;Cell membrane;Ion transport;Membrane;Reference proteome;Transmembrane;Transmembrane helix;Transport;Vacuole SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Vacuole membrane; Multi-pass membrane protein. MISCELLANEOUS: Present with 195 molecules/cell in log phase SD medium." P53839,"PROTEIN NAMES: Glyoxylate reductase 1 PROTEIN FAMILY: D-isomer specific 2-hydroxyacid dehydrogenase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. FUNCTION: Glyoxylate reductase that reversibly reduces glyoxylate to glycolate, or alternatively hydroxypyruvate to D-glycerate, using either NADPH or NADH as a cosubstrate. Does not act as a hydroxyisocaproate dehydrogenase even though it also has minor activity on alpha-ketoisocaproate. KEYWORDS: Cytoplasm;Mitochondrion;NAD;Nucleus;Oxidoreductase;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Nucleus Mitochondrion MISCELLANEOUS: Present with 3280 molecules/cell in log phase SD medium." P53844,PROTEIN NAMES: Phosphatidylinositol transfer protein PDR17 (PITP) (Phosphatidylserine transport B pathway protein 2) (Pleiotropic drug resistance protein 17) (SEC14 homolog 3) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Has phosphatidylinositol transfer activity. Involved in the regulation of the phospholipid composition of plasma- and endomembranes. Altering plasma membrane composition may provide a possible mechanism for multidrug resistance. Contributes to efficient phospholipase D1 activation and phospholipase B1 inhibition in the regulation of phospholipid turnover. Forms a complex with phosphatidylserine decarboxylase PSD2 that seems essential for maintenance of vacuolar phosphatidylethanolamine (PE) levels. KEYWORDS: Cytoplasm;Endoplasmic reticulum;Lipid transport;Microsome;Reference proteome;Transport SUBCELLULAR LOCATION: Cytoplasm Microsome MISCELLANEOUS: Present with 5040 molecules/cell in log phase SD medium. P53845,"PROTEIN NAMES: Protein transport protein YIF1 (YIP1-interacting factor 1) PROTEIN FAMILY: YIF1 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the YIF1 family. FUNCTION: Required for fusion of ER-derived vesicles with the Golgi during ER-to-Golgi protein transport. May be involved in proper membrane localization of Rab GTPases. KEYWORDS: Acetylation;Cytoplasmic vesicle;Endoplasmic reticulum;ER-Golgi transport;Golgi apparatus;Membrane;Protein transport;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein Golgi apparatus membrane ; Multi-pass membrane protein Cytoplasmic vesicle, COPII-coated vesicle Note=Also found in ER-derived COPII-coated vesicles. " P53853,PROTEIN NAMES: Vacuolar protein sorting-associated protein 75 PROTEIN FAMILY: Nucleosome assembly protein (NAP) family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the nucleosome assembly protein (NAP) family. FUNCTION: Histone chaperone which acts as a cofactor stimulating histone H3 acetylation by RTT109. Preferentially stimulates histone H3 'Lys-9' acetylation by RTT109. May also stimulate histone H3 'Lys-56' acetylation by RTT109. Assembles nucleosomes (in vitro). KEYWORDS: 3D-structure;Nucleus;Phosphoprotein;Protein transport;Reference proteome;Transport SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: Present with 3120 molecules/cell in log phase SD medium. P53865,"PROTEIN NAMES: Chaotic nuclear migration protein 67 ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Involved in the pathway that organizes the shaping and sizing of the prospore membrane (PSM) during sporulation. Required for the proper formation of the spindle pole body (SPB) outer plaque. May connect the outer plaque to the central plaque embedded in the nuclear envelope. KEYWORDS: 3D-structure;Cell cycle;Cell division;Coiled coil;Cytoplasm;Cytoskeleton;Meiosis;Phosphoprotein;Reference proteome;Sporulation SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body Note=Localizes to the meiotic outer plaque of the SPB, at the end of the meiotic spindles. " P53874,"PROTEIN NAMES: Ubiquitin carboxyl-terminal hydrolase 10 (Deubiquitinating enzyme 10) (Disrupter of telomere silencing protein 4) (Ubiquitin thioesterase 10) (Ubiquitin-specific-processing protease 10) PROTEIN FAMILY: Peptidase C19 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the peptidase C19 family. FUNCTION: Deubiquitinating enzyme involved in telomere and HM loci silencing, which is the repression of chromatin structure which leads to a stop in the transcription of nearby genes. Targets histone H2B for deubiquitination, thus helping to localize SIR2 to the telomere. At silent chromatin, including telomeres and the rDNA locus, not only maintains low H2B 'Lys-123' ubiquitination (H2BK123Ub), but also low H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). Controls the proliferating-cell nuclear antigen PCNA/POL30 deubiquitination which is crucial for keeping TLS polymerases in check as well as for down-regulating the error-free bypass. Deubiquitinates and stabilizes RPA190, the largest subunit of RNA polymerase I, to achieve optimal levels of ribosomes and cell growth. Protects also nutrient transporters such as GAP1 from ubiquitin-dependent endocytosis. KEYWORDS: 3D-structure;Chromosome;Hydrolase;Nucleus;Protease;Reference proteome;Telomere;Thiol protease;Ubl conjugation pathway SUBCELLULAR LOCATION: Nucleus Chromosome, telomere Nucleus, nucleolus Note=Preferentially localizes to silent chromatin. " P53879,"PROTEIN NAMES: GTP-binding protein RHO5 PROTEIN FAMILY: Small GTPase superfamily, Rho family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the small GTPase superfamily. Rho family. FUNCTION: Small GTPase that negatively regulates a MAP kinase branch, downstream of SLT2, of the PKC1-mediated signal transduction pathway. With its specific guanine nucleotide exchange factor (GEF), the heterodimeric complex DCK1/LMO1, relocates to mitochondria upon oxidative stress and triggers cell death. The DCK1/LMO1/RHO5 signaling module that mediates mitochondrial turnover under nitrogen starvation conditions via mitophagy. The DCK1/LMO1/RHO5 signaling module also plays a role in cell wall integrity signaling. KEYWORDS: GTP-binding;Isopeptide bond;Lipoprotein;Membrane;Methylation;Mitochondrion;Nucleotide-binding;Phosphoprotein;Prenylation;Reference proteome;Ubl conjugation SUBCELLULAR LOCATION: Membrane ; Lipid-anchor Mitochondrion Note=Localization to mitochondria occurs upon oxidative stress and is dependent of DCK1 and LMO1. MISCELLANEOUS: Present with 2180 molecules/cell in log phase SD medium." P53895,"PROTEIN NAMES: Protein ASI2 (Amino acid sensor-independent protein 2) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Part of the nuclear inner membrane (INM)-specific branch of the ER-associated degradation (ERAD) pathway, required for the elimination of misfolded proteins in the INM, a specialized ER subdomain. Required for ERG11 degradation. Negative regulator of SPS-sensor signaling. Together with ASI2 and ASI3, prevents the unprocessed precursor forms of STP1 and STP2 that escape cytoplasmic anchoring from inducing SPS-sensor-regulated genes in the absence of inducing signals. Controls amino acid permease (AAP) gene expression in response to amino acid availability, a process mediated by the transcription factors STP1 and STP1. KEYWORDS: Membrane;Nucleus;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Nucleus inner membrane ; Multi-pass membrane protein " P53899,PROTEIN NAMES: CDC48-associated ubiquitin-like/zinc finger protein 1 (CDC48-associated UBL/Zn-finger protein 1) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Promotes efficient arsenite-induced clearance of stress granules (SGs). May have a role in the ubiquitin-proteasome system (UPS) protecting cells from metalloid-induced proteotoxicity. KEYWORDS: 3D-structure;Cytoplasm;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Stress response;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cytoplasm Nucleus MISCELLANEOUS: Present with 4280 molecules/cell in log phase SD medium. P53927,"PROTEIN NAMES: Ribosome biogenesis protein 15 (Nucleolar protein 15) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Involved in the biogenesis of the 60S ribosomal subunit. Required for pre-rRNA processing and cytokinesis. Associates with the precursors of the 25S and 5.8S rRNAs. KEYWORDS: 3D-structure;Cytoplasm;Nucleus;Reference proteome;Ribonucleoprotein;Ribosome biogenesis;RNA-binding;rRNA processing SUBCELLULAR LOCATION: Cytoplasm Nucleus, nucleolus MISCELLANEOUS: Present with 4280 molecules/cell in log phase SD medium." P53933,"PROTEIN NAMES: Phosphatidate phosphatase APP1 (PAP) (Actin patch protein 1) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Mg(2+)-dependent phosphatidate (PA) phosphatase which catalyzes the dephosphorylation of PA to yield diacylglycerol. May play a role in vesicular trafficking through its PAP activity at cortical actin patches. Can also utilize diacylglycerol pyrophosphate and lyso-PA as substrates with specificity constants 4- and 7-fold lower, respectively, when compared with PA. KEYWORDS: Cytoplasm;Cytoskeleton;Glycoprotein;Hydrolase;Lipid metabolism;Reference proteome SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, actin patch MISCELLANEOUS: Present with 2289 molecules/cell in log phase SD medium." P53941,"PROTEIN NAMES: U3 small nucleolar ribonucleoprotein protein IMP4 (U3 snoRNP protein IMP4) (Interacting with MPP10 protein 4) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Required for the early cleavages at sites A0, A1 and A2 during 18S ribosomal pre-RNA processing. KEYWORDS: 3D-structure;Nucleus;Reference proteome;Ribonucleoprotein;Ribosome biogenesis;rRNA processing SUBCELLULAR LOCATION: Nucleus, nucleolus " P53954,"PROTEIN NAMES: GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase (Alpha-1,2-mannosyltransferase ALG11) (Asparagine-linked glycosylation protein 11) (Glycolipid 2-alpha-mannosyltransferase) PROTEIN FAMILY: Glycosyltransferase group 1 family, Glycosyltransferase 4 subfamily ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 4 subfamily. FUNCTION: GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. Catalyzes, on the cytoplasmic face of the endoplasmic reticulum, the addition of the fourth and fifth mannose residues to the dolichol-linked oligosaccharide chain, to produce Man(5)GlcNAc(2)-PP-dolichol core oligosaccharide. Man(5)GlcNAc(2)-PP-dolichol is a substrate for ALG3, the following enzyme in the biosynthetic pathway. PATHWAY: Protein modification; protein glycosylation. KEYWORDS: Endoplasmic reticulum;Glycosyltransferase;Membrane;Reference proteome;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Single-pass membrane protein MISCELLANEOUS: Present with 3140 molecules/cell in log phase SD medium." P53969,"PROTEIN NAMES: Sorting assembly machinery 50 kDa subunit (TOB complex 55 kDa subunit) PROTEIN FAMILY: SAM50/omp85 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the SAM50/omp85 family. FUNCTION: Component of the mitochondrial outer membrane sorting assembly machinery (SAM or TOB) complex, which is required for the sorting of proteins with complicated topology, such as beta-barrel proteins, to the mitochondrial outer membrane after import by the TOM complex. KEYWORDS: 3D-structure;Membrane;Mitochondrion;Mitochondrion outer membrane;Protein transport;Reference proteome;Transmembrane;Transmembrane beta strand;Transport SUBCELLULAR LOCATION: Mitochondrion outer membrane ; Multi-pass membrane protein " P53972,"PROTEIN NAMES: 25S rRNA (cytosine(2278)-C(5))-methyltransferase (rRNA m(5)C methyltransferase 1) PROTEIN FAMILY: Class I-like SAM-binding methyltransferase superfamily, RsmB/NOP family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family. FUNCTION: S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the C(5) position of cytosine 2278 (m5C2278) in 25S rRNA. Loss of m5C2278 in 25S rRNA results in anisomycin hypersensitivity. KEYWORDS: Methyltransferase;Nucleus;Reference proteome;RNA-binding;rRNA processing;S-adenosyl-L-methionine;Transferase SUBCELLULAR LOCATION: Nucleus, nucleolus MISCELLANEOUS: Present with 922 molecules/cell in log phase SD medium." P53974,"PROTEIN NAMES: Actin-regulating kinase 1 PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. FUNCTION: Involved in regulation of actin cytoskeleton organization and endocytosis. KEYWORDS: 3D-structure;ATP-binding;Cytoplasm;Cytoskeleton;Kinase;Nucleotide-binding;Phosphoprotein;Reference proteome;Serine/threonine-protein kinase;Transferase SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, actin patch Note=Cortical actin patches. MISCELLANEOUS: Present with 1551 molecules/cell in log phase SD medium." P53983,"PROTEIN NAMES: Probable ERAD-associated E3 ubiquitin-protein ligase ASI1 (Amino acid sensor-independent protein 3) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Part of the nuclear inner membrane (INM)-specific branch of the ER-associated degradation (ERAD) pathway, required for the elimination of misfolded proteins in the INM, a specialized ER subdomain. Required for ERG11 degradation. Negative regulator of SPS-sensor signaling. Together with ASI2 and ASI3, prevents the unprocessed precursor forms of STP1 and STP2 that escape cytoplasmic anchoring from inducing SPS-sensor-regulated genes in the absence of inducing signals. Controls amino acid permease (AAP) gene expression in response to amino acid availability, a process mediated by the transcription factors STP1 and STP1. KEYWORDS: Glycoprotein;Membrane;Metal-binding;Nucleus;Reference proteome;Transferase;Transmembrane;Transmembrane helix;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus inner membrane ; Multi-pass membrane protein " P54003,"PROTEIN NAMES: Protein SUR7 PROTEIN FAMILY: SUR7 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the SUR7 family. FUNCTION: Involved in sporulation and affects the sphingolipid composition of the plasma membrane. Probably involved in endocytosis. KEYWORDS: Cell membrane;Endocytosis;Glycoprotein;Membrane;Phosphoprotein;Reference proteome;Sporulation;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Note=Localizes at eisosomes, structures which colocalize with sites of protein and lipid endocytosis. MISCELLANEOUS: Present with 17000 molecules/cell in log phase SD medium." P54070,"PROTEIN NAMES: Mannosyltransferase KTR6 (Mannosylphosphate transferase MNN6) PROTEIN FAMILY: Glycosyltransferase 15 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the glycosyltransferase 15 family. FUNCTION: Glycosyltransferase that transfers an alpha-D-mannosyl residue from GDP-mannose into lipid-linked oligosaccharide, forming an alpha-(1->2)-D-mannosyl-D-mannose linkage. Required for addition of mannosylphosphate in yeast mannan. Recognizes any oligosaccharides with at least one alpha-1,2-linked mannobiose unit. PATHWAY: Protein modification; protein glycosylation. KEYWORDS: Glycoprotein;Glycosyltransferase;Membrane;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Single-pass type II membrane protein MISCELLANEOUS: Present with 4380 molecules/cell in log phase SD medium." P54074,"PROTEIN NAMES: ERAD-associated E3 ubiquitin-protein ligase ASI1 (Amino acid sensor-independent protein 1) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: E3 ubiquitin-protein ligase which transfers ubiquitin to substrates promoting their degradation. Part of the nuclear inner membrane (INM)-specific branch of the ER-associated degradation (ERAD) pathway, required for the elimination of misfolded proteins in the INM, a specialized ER subdomain. Required for ERG11 degradation. Negative regulator of SPS-sensor signaling. Together with ASI2 and ASI3, prevents the unprocessed precursor forms of STP1 and STP2 that escape cytoplasmic anchoring from inducing SPS-sensor-regulated genes in the absence of inducing signals. Controls amino acid permease (AAP) gene expression in response to amino acid availability, a process mediated by the transcription factors STP1 and STP1. KEYWORDS: Glycoprotein;Membrane;Metal-binding;Nucleus;Reference proteome;Transferase;Transmembrane;Transmembrane helix;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus inner membrane ; Multi-pass membrane protein " P54107,"PROTEIN NAMES: Cysteine-rich secretory protein 1 (CRISP-1) (AEG-like protein) (ARP) (Acidic epididymal glycoprotein homolog) PROTEIN FAMILY: CRISP family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the CRISP family. FUNCTION: May have a role in sperm-egg fusion and maturation. KEYWORDS: Alternative splicing;Direct protein sequencing;Disulfide bond;Glycoprotein;Reference proteome;Signal SUBCELLULAR LOCATION: Note=Located in the lumen and epithelium of distal ductus efferentes and epididymal ducts, and on the postacrosomal region of the sperm head. " P54199,"PROTEIN NAMES: Serine/threonine-protein kinase MPS1 (Monopolar spindle protein 1) (Regulatory cell proliferation kinase 1) PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. FUNCTION: Involved in the regulation of the onset of mitosis. Involved in a pathway that coordinates cell proliferation and differentiation. Implicated in spindle pole body (SPD) duplication. Dual specificity kinase that can phosphorylate serine, threonine and tyrosine residues. Phosphorylates the SPC29 and SPC110 spindle pole body components. KEYWORDS: ATP-binding;Kinase;Nucleotide-binding;Phosphoprotein;Reference proteome;Serine/threonine-protein kinase;Transferase " P54219,"PROTEIN NAMES: Chromaffin granule amine transporter (Solute carrier family 18 member 1) (Vesicular amine transporter 1) (VAT1) PROTEIN FAMILY: Major facilitator superfamily, Vesicular transporter family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the major facilitator superfamily. Vesicular transporter family. FUNCTION: [Isoform 1]: Electrogenic antiporter that exchanges one cationic monoamine with two intravesicular protons across the membrane of secretory and synaptic vesicles. Uses the electrochemical proton gradient established by the V-type proton-pump ATPase to accumulate high concentrations of monoamines inside the vesicles prior to their release via exocytosis. Transports catecholamines and indolamines with higher affinity for serotonin. Regulates the transvesicular monoaminergic gradient that determines the quantal size. Mediates presynaptic monoaminergic vesicle transport in the amygdala and prefrontal brain regions related with emotion processing in response to environmental stimuli.; FUNCTION: [Isoform 2]: Unable to uptake serotonin. KEYWORDS: Alternative splicing;Cytoplasmic vesicle;Endoplasmic reticulum;Glycoprotein;Membrane;Neurotransmitter transport;Reference proteome;Synapse;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle membrane ; Multi-pass membrane protein Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane ; Multi-pass membrane protein.; SUBCELLULAR LOCATION: [Isoform 1]: Cytoplasmic vesicle, secretory vesicle membrane ; Multi-pass membrane protein.; SUBCELLULAR LOCATION: [Isoform 2]: Endoplasmic reticulum membrane ; Multi-pass membrane protein " P54253,"PROTEIN NAMES: Ataxin-1 (Spinocerebellar ataxia type 1 protein) PROTEIN FAMILY: ATXN1 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the ATXN1 family. FUNCTION: Chromatin-binding factor that repress Notch signaling in the absence of Notch intracellular domain by acting as a CBF1 corepressor. Binds to the HEY promoter and might assist, along with NCOR2, RBPJ-mediated repression. Binds RNA in vitro. May be involved in RNA metabolism. In concert with CIC and ATXN1L, involved in brain development (By similarity). KEYWORDS: 3D-structure;Alternative splicing;Cytoplasm;DNA-binding;Isopeptide bond;Neurodegeneration;Nucleus;Phosphoprotein;Reference proteome;Repressor;RNA-binding;Spinocerebellar ataxia;Transcription;Transcription regulation;Triplet repeat expansion;Ubl conjugation SUBCELLULAR LOCATION: Cytoplasm Nucleus Note=Colocalizes with USP7 in the nucleus. MISCELLANEOUS: Self-association seems to be necessary for formation of nuclear aggregates which are associated with pathogenesis." P54397,PROTEIN NAMES: 39 kDa FK506-binding nuclear protein (Peptidyl-prolyl cis-trans isomerase) (PPIase) (Rotamase) PROTEIN FAMILY: FKBP-type PPIase family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the FKBP-type PPIase family. FUNCTION: PPIases accelerate the folding of proteins. May function in a signal transduction cascade during early development. KEYWORDS: 3D-structure;Isomerase;Nucleus;Phosphoprotein;Reference proteome;Rotamase SUBCELLULAR LOCATION: Nucleus P54401,"PROTEIN NAMES: Tubulin gamma chain (Gamma-tubulin) PROTEIN FAMILY: Tubulin family ORGANISM: Entamoeba histolytica (strain ATCC 30459 / HM-1:IMSS / ABRM) SIMILARITY: Belongs to the tubulin family. FUNCTION: Tubulin is the major constituent of microtubules (Probable). The gamma chain is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome, suggesting that it is involved in the minus-end nucleation of microtubule assembly. KEYWORDS: Cytoplasm;Cytoskeleton;GTP-binding;Microtubule;Nucleotide-binding;Nucleus;Reference proteome SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Nucleus Note=Expressed during all cell cycle phases with maximal expression at the S/G2 phases. In the nucleus, forms a ring-like structure surrounded by six equally-spaced gamma-tubulin-rich patches. " P54488,"PROTEIN NAMES: Penicillin-binding protein 2A (PBP-2B) PROTEIN FAMILY: Transpeptidase family ORGANISM: Bacillus subtilis (strain 168) SIMILARITY: Belongs to the transpeptidase family. FUNCTION: Involved in the synthesis of peptidoglycan associated with cell wall elongation, especially following spore germination. Has a partially redundant function with PBP 1 (ponA) or PBP 4 (pbpD) during spore outgrowth. Plays a redundant role with PbpH in determining the rod shape of the cell during vegetative growth and spore outgrowth. PATHWAY: Cell wall biogenesis; peptidoglycan biosynthesis. KEYWORDS: Antibiotic resistance;Carboxypeptidase;Cell membrane;Cell shape;Cell wall biogenesis/degradation;Direct protein sequencing;Hydrolase;Membrane;Peptidoglycan synthesis;Protease;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane Forespore inner membrane ; Single-pass membrane protein " P54493,PROTEIN NAMES: Rhomboid protease GluP (Intramembrane serine protease) PROTEIN FAMILY: Peptidase S54 family ORGANISM: Bacillus subtilis (strain 168) SIMILARITY: Belongs to the peptidase S54 family. FUNCTION: Rhomboid-type serine protease that catalyzes intramembrane proteolysis. Important for normal cell division and sporulation. May act as a glucose exporter. KEYWORDS: 3D-structure;Cell membrane;Hydrolase;Membrane;Protease;Reference proteome;Repeat;Serine protease;TPR repeat;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein P54688,"PROTEIN NAMES: Branched-chain-amino-acid aminotransferase, cytosolic (BCAT) PROTEIN FAMILY: Class-IV pyridoxal-phosphate-dependent aminotransferase family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family. FUNCTION: Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine. KEYWORDS: Amino-acid biosynthesis;Aminotransferase;Branched-chain amino acid biosynthesis;Cytoplasm;Pyridoxal phosphate;Reference proteome;Transferase SUBCELLULAR LOCATION: Cytoplasm. " P54738,"PROTEIN NAMES: Serine/threonine-protein kinase pkn6 PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family ORGANISM: Myxococcus xanthus SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. FUNCTION: Pkn5 and pkn6 may have reciprocal roles in growth and development. Pkn6 may be a transmembrane sensor of external signals for development. KEYWORDS: ATP-binding;Cell membrane;Kinase;Membrane;Nucleotide-binding;Phosphoprotein;Repeat;Serine/threonine-protein kinase;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane; Single-pass membrane protein. " P54785,"PROTEIN NAMES: Transcriptional activator/repressor MOT3 (Hypoxic gene repressor protein 7) (Modulator of transcription protein 3) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Transcription factor that affects the expression of a large set of genes. Recognizes and binds to the consensus sequence 5'-[CAT]AGG[TC]A-3' in the promoter region. Plays a major role in the repression of a specific subset of hypoxic genes (e.g. ANB1, DAN1 and HEM13) under aerobic conditions. Acts synergistically with the transcription factor ROX1 to recruit the general repression complex SSN6-TUP1 to the promoter of hypoxic genes. Represses transcription of ergosterol biosynthetic genes. Negatively regulates pheromone-induced gene expression. Can act as a transcriptional activator (e.g. of genes like CYC1, SUC2 and the Ty long terminal repeat delta promoter). KEYWORDS: Acetylation;Activator;Amyloid;Metal-binding;Nucleus;Prion;Reference proteome;Repeat;Repressor;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: [MOT3+] is the prion form of MOT3. [MOT3+] is the result of a conformational change of the cellular MOT3 protein that becomes self-propagating and infectious. This conformational change generates a form of MOT3 that assembles into amyloid fibrils. [MOT3+]-aggregates sequester soluble MOT3, resulting in a loss-of-function phenotype for MOT3. [MOT3+] can be cured by GdnHCl and by inactivation of the molecular chaperone HSP104, which is required for [MOT3+] propagation. It is speculated that prion properties of transcription factors may generate an optimized phenotypic heterogeneity that buffers yeast populations against diverse environmental insults.; MISCELLANEOUS: Present with 1690 molecules/cell in log phase SD medium." P54802,PROTEIN NAMES: Alpha-N-acetylglucosaminidase (N-acetyl-alpha-glucosaminidase) (NAG) [Cleaved into: Alpha-N-acetylglucosaminidase 82 kDa form; Alpha-N-acetylglucosaminidase 77 kDa form] PROTEIN FAMILY: Glycosyl hydrolase 89 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the glycosyl hydrolase 89 family. FUNCTION: Involved in the degradation of heparan sulfate. KEYWORDS: 3D-structure;Charcot-Marie-Tooth disease;Direct protein sequencing;Disease variant;Glycoprotein;Glycosidase;Hydrolase;Lysosome;Mucopolysaccharidosis;Neurodegeneration;Neuropathy;Reference proteome;Signal SUBCELLULAR LOCATION: Lysosome. P54867,"PROTEIN NAMES: Protein SLG1 (Cell wall integrity and stress response component 1) (Synthetic lethal with GAP protein 1) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Plays a role during G1 to regulate entering or exiting the cell cycle. Involved in stress responses. Has a role in cell wall integrity signaling. Activates ROM1 or ROM2 catalyzed guanine nucleotide exchange toward RHO1. Important regulator of the actin cytoskeleton rearrangements in conditions of cell wall expansion and membrane stretching. Specifically required for the actin reorganization induced by hypo-osmotic shock. Multicopy suppressor of 1,3-beta-glucan synthase (GS). Activates GS upstream of RHO1. Acts positively on the PKC1-MAPK pathway. Activates transiently SLT2 during alkaline stress, which leads to an increase in the expression of several specific genes. KEYWORDS: 3D-structure;Cell cycle;Cell membrane;Cell wall biogenesis/degradation;Glycoprotein;Membrane;Phosphoprotein;Reference proteome;Signal;Stress response;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein MISCELLANEOUS: Present with 664 molecules/cell in log phase SD medium." P54987,"PROTEIN NAMES: Cis-aconitate decarboxylase (CAD) (Aconitate decarboxylase) (Aconitate decarboxylase 1) (Cis-aconitic acid decarboxylase) (Immune-responsive gene 1 protein) PROTEIN FAMILY: PrpD family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the PrpD family. FUNCTION: Cis-aconitate decarboxylase that catalyzes production of itaconate and is involved in the inhibition of the inflammatory response. Acts as a negative regulator of the Toll-like receptors (TLRs)-mediated inflammatory innate response by stimulating the tumor necrosis factor alpha-induced protein TNFAIP3 expression via reactive oxygen species (ROS) in LPS-tolerized macrophages. Involved in antimicrobial response of innate immune cells; ACOD1-mediated itaconic acid production contributes to the antimicrobial activity of macrophages by generating itaconate, leading to alkylation of proteins, such as TFEB. Involved in antiviral response following infection by flavivirus in neurons: ACOD1-mediated itaconate production inhibits the activity of succinate dehydrogenase, generating a metabolic state in neurons that suppresses replication of viral genomes. Plays a role in the embryo implantation. KEYWORDS: 3D-structure;Antimicrobial;Immunity;Inflammatory response;Innate immunity;Lyase;Mitochondrion;Reference proteome SUBCELLULAR LOCATION: Mitochondrion " P54997,"PROTEIN NAMES: 2'-hydroxybiphenyl-2-sulfinate desulfinase (2-(2-hydroxyphenyl)benzenesulfinate desulfinase) (HPBS desulfinase) (Dibenzothiophene desulfurization enzyme B) PROTEIN FAMILY: DszB desulfinase family ORGANISM: Rhodococcus sp. (strain ATCC 53968 / IGTS8) SIMILARITY: Belongs to the DszB desulfinase family. FUNCTION: Catalyzes the third and final step of the '4S' desulfurization pathway that removes covalently bound sulfur from dibenzothiophene (DBT) without breaking carbon-carbon bonds. Oxidizes 2-(2'-hydroxyphenyl)benzene sulphinate (HBPS) to 2-hydroxybiphenyl (HBP) plus sulfite. The rate-limiting step of the '4S' desulfurization pathway. The pathway substrate specificity has been augmented using mutagenesis, however no mutations allowed use of alkylated thiophenes. PATHWAY: Sulfur metabolism; dibenzothiophene degradation. KEYWORDS: 3D-structure;Cytoplasm;Hydrolase;Monooxygenase;Oxidoreductase;Plasmid SUBCELLULAR LOCATION: Cytoplasm " P54998,"PROTEIN NAMES: Dibenzothiophene monooxygenase (DBT monooxygenase) (DBT-MO) (DBT sulfur dioxygenase) (Dibenzothiophene desulfurization enzyme C) (Sulfide/sulfoxide monooxygenase) PROTEIN FAMILY: DszC flavin monooxygenase family ORGANISM: Rhodococcus sp. (strain ATCC 53968 / IGTS8) SIMILARITY: Belongs to the DszC flavin monooxygenase family. FUNCTION: Catalyzes the first step of the '4S' desulfurization pathway that removes covalently bound sulfur from dibenzothiophene (DBT) without breaking carbon-carbon bonds. Sulfur dioxygenase which converts DBT to DBT-sulfone (DBTO2 or DBT 5,5-dioxide) in a stepwise manner. In DBTO (dibenzothiophene-5-oxide) was reported not to be a substrate, in it is reported to be a substrate. Can also use benzyl sulfide and benzyl sulfoxide as substrates, although benzyl sulfoxide is a poor substrate. The pathway substrate specificity has been augmented using mutagenesis, however no mutations allowed use of alkylated thiophenes. PATHWAY: Sulfur metabolism; dibenzothiophene degradation. KEYWORDS: Cytoplasm;Flavoprotein;FMN;Monooxygenase;Nucleotide-binding;Oxidoreductase;Plasmid SUBCELLULAR LOCATION: Cytoplasm " P55055,"PROTEIN NAMES: Oxysterols receptor LXR-beta (Liver X receptor beta) (Nuclear receptor NER) (Nuclear receptor subfamily 1 group H member 2) (Ubiquitously-expressed nuclear receptor) PROTEIN FAMILY: Nuclear hormone receptor family, NR1 subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the nuclear hormone receptor family. NR1 subfamily. FUNCTION: Nuclear receptor that exhibits a ligand-dependent transcriptional activation activity. Binds preferentially to double-stranded oligonucleotide direct repeats having the consensus half-site sequence 5'-AGGTCA-3' and 4-nt spacing (DR-4). Regulates cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8; DLDLR and LRP8. Interplays functionally with RORA for the regulation of genes involved in liver metabolism (By similarity). Induces LPCAT3-dependent phospholipid remodeling in endoplasmic reticulum (ER) membranes of hepatocytes, driving SREBF1 processing and lipogenesis (By similarity). Via LPCAT3, triggers the incorporation of arachidonate into phosphatidylcholines of ER membranes, increasing membrane dynamics and enabling triacylglycerols transfer to nascent very low-density lipoprotein (VLDL) particles (By similarity). Via LPCAT3 also counteracts lipid-induced ER stress response and inflammation, likely by modulating SRC kinase membrane compartmentalization and limiting the synthesis of lipid inflammatory mediators (By similarity). Plays an anti-inflammatory role during the hepatic acute phase response by acting as a corepressor: inhibits the hepatic acute phase response by preventing dissociation of the N-Cor corepressor complex. KEYWORDS: 3D-structure;Activator;Alternative splicing;DNA-binding;Isopeptide bond;Metal-binding;Nucleus;Receptor;Reference proteome;Transcription;Transcription regulation;Ubl conjugation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus " P55058,"PROTEIN NAMES: Phospholipid transfer protein (Lipid transfer protein II) PROTEIN FAMILY: BPI/LBP/Plunc superfamily, BPI/LBP family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the BPI/LBP/Plunc superfamily. BPI/LBP family. FUNCTION: Mediates the transfer of phospholipids and free cholesterol from triglyceride-rich lipoproteins (low density lipoproteins or LDL and very low density lipoproteins or VLDL) into high-density lipoproteins (HDL) as well as the exchange of phospholipids between triglyceride-rich lipoproteins themselves. Facilitates the transfer of a spectrum of different lipid molecules, including diacylglycerol, phosphatidic acid, sphingomyelin, phosphatidylcholine, phosphatidylinositol, phosphatidylglycerol, cerebroside and phosphatidyl ethanolamine. Plays an important role in HDL remodeling which involves modulating the size and composition of HDL. Also plays a key role in the uptake of cholesterol from peripheral cells and tissues that is subsequently transported to the liver for degradation and excretion. Two distinct forms of PLTP exist in plasma: an active form that can transfer phosphatidylcholine from phospholipid vesicles to HDL, and an inactive form that lacks this capability. KEYWORDS: Alternative splicing;Direct protein sequencing;Disulfide bond;Glycoprotein;Lipid transport;Nucleus;Reference proteome;Secreted;Signal;Transport SUBCELLULAR LOCATION: Secreted Nucleus Note=Nuclear export is XPO1/CRM1-dependent. " P55086,"PROTEIN NAMES: Proteinase-activated receptor 2 (PAR-2) (Coagulation factor II receptor-like 1) (G-protein coupled receptor 11) (Thrombin receptor-like 1) PROTEIN FAMILY: G-protein coupled receptor 1 family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the G-protein coupled receptor 1 family. FUNCTION: Receptor for trypsin and trypsin-like enzymes coupled to G proteins. Its function is mediated through the activation of several signaling pathways including phospholipase C (PLC), intracellular calcium, mitogen-activated protein kinase (MAPK), I-kappaB kinase/NF-kappaB and Rho. Can also be transactivated by cleaved F2r/Par1. Involved in modulation of inflammatory responses and regulation of innate and adaptive immunity, and acts as a sensor for proteolytic enzymes generated during infection. Generally is promoting inflammation. Can signal synergistically with Tlr4 and probably Tlr2 in inflammatory responses and modulates Tlr3 signaling. Has a protective role in establishing the endothelial barrier; the activity involves coagulation factor X. Regulates endothelial cell barrier integrity during neutrophil extravasation, probably following proteolytic cleavage by PRTN3 (By similarity). Proposed to have a bronchoprotective role in airway epithelium, but also shown to compromise the airway epithelial barrier by interrupting E-cadherin adhesion. Involved in the regulation of vascular tone; activation results in hypotension presumably mediated by vasodilation. Associates with a subset of G proteins alpha subunits such as GNAQ, GNA11, GNA14, GNA12 and GNA13, but probably not with G(o)-alpha, G(i) subunit alpha-1 and G(i) subunit alpha-2. Believed to be a class B receptor which internalizes as a complex with arrestin and traffic with it to endosomal vesicles, presumably as desensitized receptor, for extended periods of time. Mediates inhibition of TNF-alpha stimulated JNK phosphorylation via coupling to GNAQ and GNA11; the function involves dissociation of Ripk1 and Tradd from Tnfr1. Mediates phosphorylation of nuclear factor NF-kappa-B RELA subunit at 'Ser-536'; the function involves Ikbkb and is predominantly independent of G proteins. Involved in cellular migration. Involved in cytoskeletal rearrangement and chemotaxis through beta-arrestin-promoted scaffolds; the function is independent of GNAQ and GNA11 and involves promotion of cofilin dephosphorylation and actin filament severing. Induces redistribution of Cops5 from the plasma membrane to the cytosol and activation of the JNK cascade is mediated by Cops5. Involved in the recruitment of leukocytes to the sites of inflammation and is the major PAR receptor capable of modulating eosinophil function such as pro-inflammatory cytokine secretion, superoxide production and degranulation. During inflammation promotes dendritic cell maturation, trafficking to the lymph nodes and subsequent T-cell activation. Involved in antimicrobial response of innate immune cells; activation enhances phagocytosis of Gram-positive and killing of Gram-negative bacteria. Acts synergistically with interferon-gamma in enhancing antiviral responses (By similarity). KEYWORDS: Cell membrane;Disulfide bond;G-protein coupled receptor;Glycoprotein;Immunity;Inflammatory response;Innate immunity;Lipoprotein;Membrane;Palmitate;Phosphoprotein;Receptor;Reference proteome;Signal;Transducer;Transmembrane;Transmembrane helix;Ubl conjugation SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein. MISCELLANEOUS: Synthetic PAR agonist peptides (APs) that mimic the first six amino acids of the newly formed N-terminus activate the native, uncleaved receptor nonenzymatically by binding directly to the corresponding second extracellular loop to mediate signaling." P55100,"PROTEIN NAMES: Peroxisomal bifunctional enzyme (PBE) (PBFE) (Multifunctional enzyme 1) (MFE1) [Includes: Enoyl-CoA hydratase/3,2-trans-enoyl-CoA isomerase ; 3-hydroxyacyl-CoA dehydrogenase ] PROTEIN FAMILY: Enoyl-CoA hydratase/isomerase family; 3-hydroxyacyl-CoA dehydrogenase family ORGANISM: Cavia porcellus (Guinea pig) SIMILARITY: In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.; SIMILARITY: In the C-terminal section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family. FUNCTION: Peroxisomal trifunctional enzyme possessing 2-enoyl-CoA hydratase, 3-hydroxyacyl-CoA dehydrogenase, and delta 3, delta 2-enoyl-CoA isomerase activities. Catalyzes two of the four reactions of the long chain fatty acids peroxisomal beta-oxidation pathway (By similarity). Can also use branched-chain fatty acids such as 2-methyl-2E-butenoyl-CoA as a substrate, which is hydrated into (2S,3S)-3-hydroxy-2-methylbutanoyl-CoA (By similarity). Optimal isomerase for 2,5 double bonds into 3,5 form isomerization in a range of enoyl-CoA species. Also able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species (By similarity). Regulates the amount of medium-chain dicarboxylic fatty acids which are essential regulators of all fatty acid oxidation pathways (By similarity). Also involved in the degradation of long-chain dicarboxylic acids through peroxisomal beta-oxidation (By similarity). PATHWAY: Lipid metabolism; fatty acid beta-oxidation. KEYWORDS: Acetylation;Fatty acid metabolism;Isomerase;Lipid metabolism;Lyase;Multifunctional enzyme;NAD;Oxidoreductase;Peroxisome;Reference proteome SUBCELLULAR LOCATION: Peroxisome " P55107,PROTEIN NAMES: Growth/differentiation factor 10 (GDF-10) (Bone morphogenetic protein 3B) (BMP-3B) (Bone-inducing protein) (BIP) PROTEIN FAMILY: TGF-beta family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the TGF-beta family. FUNCTION: Growth factor involved in osteogenesis and adipogenesis. Plays an inhibitory role in the process of osteoblast differentiation via SMAD2/3 pathway. Plays an inhibitory role in the process of adipogenesis. KEYWORDS: Cleavage on pair of basic residues;Cytokine;Disulfide bond;Glycoprotein;Growth factor;Osteogenesis;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted P55199,"PROTEIN NAMES: RNA polymerase II elongation factor ELL (Eleven-nineteen lysine-rich leukemia protein) PROTEIN FAMILY: ELL/occludin family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the ELL/occludin family. FUNCTION: Elongation factor component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. Elongation factor component of the little elongation complex (LEC), a complex required to regulate small nuclear RNA (snRNA) gene transcription by RNA polymerase II and III. Specifically required for stimulating the elongation step of RNA polymerase II- and III-dependent snRNA gene transcription. ELL also plays an early role before its assembly into in the SEC complex by stabilizing RNA polymerase II recruitment/initiation and entry into the pause site. Required to stabilize the pre-initiation complex and early elongation. KEYWORDS: 3D-structure;Acetylation;Chromosomal rearrangement;Nucleus;Phosphoprotein;Proto-oncogene;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus Nucleus speckle Nucleus, Cajal body Note=Colocalizes with EAF2 to nuclear speckles. Colocalizes with coilin in subnuclear cajal and histone locus bodies. Translocates in the LEC complex to cajal and histone locus bodies at snRNA genes in a ICE1-dependent manner. Associates to transcriptionally active chromatin at snRNA genes. " P55210,"PROTEIN NAMES: Caspase-7 (CASP-7) (Apoptotic protease Mch-3) (CMH-1) (ICE-like apoptotic protease 3) (ICE-LAP3) [Cleaved into: Caspase-7 subunit p20; Caspase-7 subunit p11] PROTEIN FAMILY: Peptidase C14A family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the peptidase C14A family. FUNCTION: Thiol protease involved in different programmed cell death processes, such as apoptosis, pyroptosis or granzyme-mediated programmed cell death, by proteolytically cleaving target proteins. Has a marked preference for Asp-Glu-Val-Asp (DEVD) consensus sequences, with some plasticity for alternate non-canonical sequences. Its involvement in the different programmed cell death processes is probably determined by upstream proteases that activate CASP7 (By similarity). Acts as an effector caspase involved in the execution phase of apoptosis: following cleavage and activation by initiator caspases (CASP8, CASP9 and/or CASP10), mediates execution of apoptosis by catalyzing cleavage of proteins, such as CLSPN, PARP1, PTGES3 and YY1. Compared to CASP3, acts as a minor executioner caspase and cleaves a limited set of target proteins. Acts as a key regulator of the inflammatory response in response to bacterial infection by catalyzing cleavage and activation of the sphingomyelin phosphodiesterase SMPD1 in the extracellular milieu, thereby promoting membrane repair. Regulates pyroptosis in intestinal epithelial cells: cleaved and activated by CASP1 in response to S.typhimurium infection, promoting its secretion to the extracellular milieu, where it catalyzes activation of SMPD1, generating ceramides that repair membranes and counteract the action of gasdermin-D (GSDMD) pores (By similarity). Regulates granzyme-mediated programmed cell death in hepatocytes: cleaved and activated by granzyme B (GZMB) in response to bacterial infection, promoting its secretion to the extracellular milieu, where it catalyzes activation of SMPD1, generating ceramides that repair membranes and counteract the action of perforin (PRF1) pores (By similarity). Following cleavage by CASP1 in response to inflammasome activation, catalyzes processing and inactivation of PARP1, alleviating the transcription repressor activity of PARP1. Acts as an inhibitor of type I interferon production during virus-induced apoptosis by mediating cleavage of antiviral proteins CGAS, IRF3 and MAVS, thereby preventing cytokine overproduction (By similarity). Cleaves and activates sterol regulatory element binding proteins (SREBPs). Cleaves phospholipid scramblase proteins XKR4, XKR8 and XKR9 (By similarity). In case of infection, catalyzes cleavage of Kaposi sarcoma-associated herpesvirus protein ORF57, thereby preventing expression of viral lytic genes.; FUNCTION: [Isoform Beta]: Lacks enzymatic activity. KEYWORDS: 3D-structure;Acetylation;Allosteric enzyme;Alternative splicing;Apoptosis;Cytoplasm;Hydrolase;Nucleus;Phosphoprotein;Protease;Reference proteome;RNA-binding;Secreted;Thiol protease;Zymogen SUBCELLULAR LOCATION: Cytoplasm, cytosol Nucleus Secreted, extracellular space Note=Following cleavage and activation by CASP1 or granzyme B (GZMB), secreted into the extracellular milieu by passing through the gasdermin-D (GSDMD) pores or perforin (PRF1) pore, respectively. " P55212,"PROTEIN NAMES: Caspase-6 (CASP-6) (CSP-6) (Apoptotic protease Mch-2) [Cleaved into: Caspase-6 subunit p18 (Caspase-6 subunit p20); Caspase-6 subunit p11 (Caspase-6 subunit p10)] PROTEIN FAMILY: Peptidase C14A family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the peptidase C14A family. FUNCTION: Cysteine protease that plays essential roles in programmed cell death, axonal degeneration, development and innate immunity. Acts as a non-canonical executioner caspase during apoptosis: localizes in the nucleus and cleaves the nuclear structural protein NUMA1 and lamin A/LMNA thereby inducing nuclear shrinkage and fragmentation. Lamin-A/LMNA cleavage is required for chromatin condensation and nuclear disassembly during apoptotic execution. Acts as a regulator of liver damage by promoting hepatocyte apoptosis: in absence of phosphorylation by AMP-activated protein kinase (AMPK), catalyzes cleavage of BID, leading to cytochrome c release, thereby participating in nonalcoholic steatohepatitis. Cleaves PARK7/DJ-1 in cells undergoing apoptosis (By similarity). Involved in intrinsic apoptosis by mediating cleavage of RIPK1. Furthermore, cleaves many transcription factors such as NF-kappa-B and cAMP response element-binding protein/CREBBP. Cleaves phospholipid scramblase proteins XKR4 and XKR9 (By similarity). In addition to apoptosis, involved in different forms of programmed cell death. Plays an essential role in defense against viruses by acting as a central mediator of the ZBP1-mediated pyroptosis, apoptosis, and necroptosis (PANoptosis), independently of its cysteine protease activity. PANoptosis is a unique inflammatory programmed cell death, which provides a molecular scaffold that allows the interactions and activation of machinery required for inflammasome/pyroptosis, apoptosis and necroptosis. Mechanistically, interacts with RIPK3 and enhances the interaction between RIPK3 and ZBP1, leading to ZBP1-mediated inflammasome activation and cell death. Plays an essential role in axon degeneration during axon pruning which is the remodeling of axons during neurogenesis but not apoptosis (By similarity). Regulates B-cell programs both during early development and after antigen stimulation (By similarity).; FUNCTION: (Microbial infection) Proteolytically cleaves the N protein of coronaviruses such as MERS-CoV and SARS-CoV. The cleavage of MERS-CoV N-protein leads to two fragments and modulates coronavirus replication by regulating IFN signaling. The two fragments produced by the cleavage interact with IRF3 inhibiting its nuclear translocation after activation and reduce the expression of IFNB and IFN-stimulated genes. The same mechanism seems to be used by other coronaviruses such as SARS-CoV and SARS-CoV-2 to enhance their replication. KEYWORDS: 3D-structure;Alternative splicing;Apoptosis;Autocatalytic cleavage;Cytoplasm;Hydrolase;Lipoprotein;Nucleus;Palmitate;Phosphoprotein;Protease;Reference proteome;Thiol protease;Zymogen SUBCELLULAR LOCATION: Cytoplasm Nucleus " P55217,"PROTEIN NAMES: Cystathionine gamma-synthase 1, chloroplastic (AtCGS1) (METHIONINE OVERACCUMULATION 1) (O-succinylhomoserine (thiol)-lyase) PROTEIN FAMILY: Trans-sulfuration enzymes family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the trans-sulfuration enzymes family. FUNCTION: Catalyzes the first committed step of methionine (Met) biosynthesis. Catalyzes the formation of L-cystathionine from homoserine esters and L-cysteine, via a gamma-replacement reaction. Substrate preference for cystathionine synthesis is O-phospho-L-homoserine (OPH) > O(4)-succinyl-L-homoserine (OSH) >> O-acetyl-L-homoserine (OAH). Is able, at extremely low rate, to catalyze a gamma-elimination of OPH in the absence of cysteine to produce inorganic phosphate (Pi), 2-oxobutanoate and ammonia. PATHWAY: Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-cystathionine from O-succinyl-L-homoserine: step 1/1. KEYWORDS: Amino-acid biosynthesis;Chloroplast;Direct protein sequencing;Methionine biosynthesis;Plastid;Pyridoxal phosphate;Reference proteome;Transferase;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast MISCELLANEOUS: A DNA region of the first exon coding for a conserved motif of 11 amino acids in CGS1 (positions 77-87) is required for post-transcriptional autoregulation and acts in cis to down-regulate its own mRNA stability in response to excess methionine. This conserved motif is dispensable for CGS enzymatic activity and only found in plant CGSs. It is unclear whether the transit peptide cleavage site is between Phe-68 and Val-69 or Ala-90 and Ala-91." P55263,PROTEIN NAMES: Adenosine kinase (AK) (Adenosine 5'-phosphotransferase) PROTEIN FAMILY: Carbohydrate kinase PfkB family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the carbohydrate kinase PfkB family. FUNCTION: Catalyzes the phosphorylation of the purine nucleoside adenosine at the 5' position in an ATP-dependent manner. Serves as a potential regulator of concentrations of extracellular adenosine and intracellular adenine nucleotides. PATHWAY: Purine metabolism; AMP biosynthesis via salvage pathway; AMP from adenosine: step 1/1. KEYWORDS: 3D-structure;Acetylation;Alternative splicing;ATP-binding;Cytoplasm;Direct protein sequencing;Disease variant;Kinase;Magnesium;Metal-binding;Nucleotide-binding;Nucleus;Phosphoprotein;Purine salvage;Reference proteome;Transferase SUBCELLULAR LOCATION: [Isoform 1]: Nucleus.; SUBCELLULAR LOCATION: [Isoform 2]: Cytoplasm P55291,PROTEIN NAMES: Cadherin-15 (Cadherin-14) (Muscle cadherin) (M-cadherin) ORGANISM: Homo sapiens (Human) FUNCTION: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. M-cadherin is part of the myogenic program and may provide a trigger for terminal muscle differentiation. KEYWORDS: Calcium;Cell adhesion;Cell membrane;Chromosomal rearrangement;Cleavage on pair of basic residues;Disease variant;Glycoprotein;Intellectual disability;Membrane;Metal-binding;Reference proteome;Repeat;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. P55818,PROTEIN NAMES: Bifunctional protein MdtA [Includes: NADP-dependent methylenetetrahydromethanopterin dehydrogenase ; Methylenetetrahydrofolate dehydrogenase ] ORGANISM: Methylorubrum extorquens (strain ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1) (Methylobacterium extorquens) FUNCTION: Catalyzes the dehydrogenation of methylene-H(4)MPT. Can also catalyze the reversible dehydrogenation of methylene-H(4)F with 20-fold lower catalytic efficiency. PATHWAY: One-carbon metabolism; formaldehyde degradation; formate from formaldehyde (H(4)MPT route): step 2/5. KEYWORDS: 3D-structure;Cytoplasm;Direct protein sequencing;NADP;One-carbon metabolism;Oxidoreductase;Reference proteome SUBCELLULAR LOCATION: Cytoplasm P55916,"PROTEIN NAMES: Putative mitochondrial transporter UCP3 (Solute carrier family 25 member 9) (Uncoupling protein-3) (UCP 3) PROTEIN FAMILY: Mitochondrial carrier family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the mitochondrial carrier (TC 2.A.29) family. FUNCTION: Putative transmembrane transporter that plays a role in mitochondrial metabolism via an as yet unclear mechanism. Originally, this mitochondrial protein was thought to act as a proton transmembrane transporter from the mitochondrial intermembrane space into the matrix, causing proton leaks through the inner mitochondrial membrane, thereby uncoupling mitochondrial membrane potential generation from ATP synthesis. However, this function is controversial and uncoupling may not be the function, or at least not the main function, but rather a consequence of more conventional metabolite transporter activity. KEYWORDS: Alternative splicing;Diabetes mellitus;Disease variant;Membrane;Mitochondrion;Mitochondrion inner membrane;Obesity;Reference proteome;Repeat;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Mitochondrion inner membrane ; Multi-pass membrane protein " P56202,PROTEIN NAMES: Cathepsin W (Lymphopain) PROTEIN FAMILY: Peptidase C1 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the peptidase C1 family. FUNCTION: May have a specific function in the mechanism or regulation of T-cell cytolytic activity.; FUNCTION: (Microbial infection) Plays a role during influenza virus infection in lungs cells ex vivo. Acts at the level of virus entering host cytoplasm from late endosome. KEYWORDS: Direct protein sequencing;Disulfide bond;Endoplasmic reticulum;Glycoprotein;Hydrolase;Protease;Reference proteome;Signal;Thiol protease;Zymogen SUBCELLULAR LOCATION: Endoplasmic reticulum P56206,PROTEIN NAMES: Glycine--tRNA ligase (Glycyl-tRNA synthetase) (GlyRS) PROTEIN FAMILY: Class-II aminoacyl-tRNA synthetase family ORGANISM: Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family. FUNCTION: Catalyzes the attachment of glycine to tRNA(Gly). KEYWORDS: 3D-structure;Aminoacyl-tRNA synthetase;ATP-binding;Cytoplasm;Ligase;Nucleotide-binding;Protein biosynthesis;Reference proteome SUBCELLULAR LOCATION: Cytoplasm P56270,"PROTEIN NAMES: Myc-associated zinc finger protein (MAZI) (Pur-1) (Purine-binding transcription factor) (Serum amyloid A-activating factor-1) (SAF-1) (Transcription factor Zif87) (ZF87) (Zinc finger protein 801) ORGANISM: Homo sapiens (Human) FUNCTION: Transcriptional regulator, potentially with dual roles in transcription initiation and termination.; FUNCTION: [Isoform 1]: Binds DNA and functions as a transcriptional activator. Binds to two G/A-rich sites, ME1a1 and ME1a2, within the MYC promoter having greater affinity for the former. Also binds to multiple G/C-rich sites within the promoter of the Sp1 family of transcription factors.; FUNCTION: [Isoform 2]: Binds DNA and functions as a transcriptional activator. Inhibits MAZ isoform 1-mediated transcription.; FUNCTION: [Isoform 3]: Binds DNA and functions as a transcriptional activator. KEYWORDS: Alternative splicing;DNA-binding;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Repeat;RNA-binding;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Note=In brains of Alzheimer disease patients, present in a plaque-like structures. MISCELLANEOUS: [Isoform 2]: May act as a dominant negative of isoform 1. Reduced expression during inflammatory conditions.; MISCELLANEOUS: [Isoform 3]: The transactivation potential of isoform 3 is much greater than that of the predominantly expressed isoform 1." P56601,"PROTEIN NAMES: Protoporphyrinogen oxidase (PPOX) PROTEIN FAMILY: Protoporphyrinogen/coproporphyrinogen oxidase family, Protoporphyrinogen oxidase subfamily ORGANISM: Myxococcus xanthus SIMILARITY: Belongs to the protoporphyrinogen/coproporphyrinogen oxidase family. Protoporphyrinogen oxidase subfamily. FUNCTION: Catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX. Does not oxidize coproporphyrinogen III. Involved in the classical protoporphyrin-dependent (PPD) heme b biosynthesis. PATHWAY: Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; protoporphyrin-IX from protoporphyrinogen-IX: step 1/1. KEYWORDS: 3D-structure;Cell membrane;Cytoplasm;FAD;Flavoprotein;Heme biosynthesis;Membrane;Oxidoreductase SUBCELLULAR LOCATION: Cytoplasm Cell membrane Note=Membrane-associated. " P56962,"PROTEIN NAMES: Syntaxin-17 PROTEIN FAMILY: Syntaxin family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the syntaxin family. FUNCTION: SNAREs, soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion. STX17 is a SNARE of the autophagosome involved in autophagy through the direct control of autophagosome membrane fusion with the lysosome membrane. May also play a role in the early secretory pathway where it may maintain the architecture of the endoplasmic reticulum-Golgi intermediate compartment/ERGIC and Golgi and/or regulate transport between the endoplasmic reticulum, the ERGIC and the Golgi. KEYWORDS: 3D-structure;Acetylation;Autophagy;Coiled coil;Cytoplasm;Cytoplasmic vesicle;Endoplasmic reticulum;ER-Golgi transport;Host-virus interaction;Membrane;Mitochondrion;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein Smooth endoplasmic reticulum membrane ; Multi-pass membrane protein Endoplasmic reticulum-Golgi intermediate compartment membrane ; Multi-pass membrane protein Cytoplasmic vesicle, autophagosome membrane ; Multi-pass membrane protein Cytoplasmic vesicle, COPII-coated vesicle membrane ; Multi-pass membrane protein Cytoplasm, cytosol Mitochondrion membrane ; Multi-pass membrane protein Note=Has a hairpin-like insertion into membranes. Localizes to the completed autophagosome membrane upon cell starvation. " P57057,"PROTEIN NAMES: Glucose-6-phosphate exchanger SLC37A1 (Glycerol-3-phosphate permease) (G-3-P permease) (Solute carrier family 37 member 1) PROTEIN FAMILY: Major facilitator superfamily, Organophosphate:Pi antiporter (OPA) family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the major facilitator superfamily. Organophosphate:Pi antiporter (OPA) (TC 2.A.1.4) family. FUNCTION: Inorganic phosphate and glucose-6-phosphate antiporter. May transport cytoplasmic glucose-6-phosphate into the lumen of the endoplasmic reticulum and translocate inorganic phosphate into the opposite direction. Independent of a lumenal glucose-6-phosphatase. May not play a role in homeostatic regulation of blood glucose levels. KEYWORDS: Antiport;Endoplasmic reticulum;Glycoprotein;Membrane;Reference proteome;Sugar transport;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein " P57727,"PROTEIN NAMES: Transmembrane protease serine 3 (Serine protease TADG-12) (Tumor-associated differentially-expressed gene 12 protein) PROTEIN FAMILY: Peptidase S1 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the peptidase S1 family. FUNCTION: Probable serine protease that plays a role in hearing. Acts as a permissive factor for cochlear hair cell survival and activation at the onset of hearing and is required for saccular hair cell survival (By similarity). Activates ENaC (in vitro). KEYWORDS: Alternative splicing;Autocatalytic cleavage;Deafness;Disease variant;Disulfide bond;Endoplasmic reticulum;Glycoprotein;Hydrolase;Membrane;Non-syndromic deafness;Protease;Reference proteome;Serine protease;Signal-anchor;Transmembrane;Transmembrane helix;Zymogen SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Single-pass type II membrane protein MISCELLANEOUS: [Isoform 6]: Has a predicted N-terminal signal sequence, indicating it may be secreted. Expressed in retina, lung, liver, pancreas, placenta and kidney." P57790,"PROTEIN NAMES: Kelch-like ECH-associated protein 1 (Cytosolic inhibitor of Nrf2) (INrf2) PROTEIN FAMILY: KEAP1 family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the KEAP1 family. FUNCTION: Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that regulates the response to oxidative stress by targeting NFE2L2/NRF2 for ubiquitination. KEAP1 acts as a key sensor of oxidative and electrophilic stress: in normal conditions, the BCR(KEAP1) complex mediates ubiquitination and degradation of NFE2L2/NRF2, a transcription factor regulating expression of many cytoprotective genes. In response to oxidative stress, different electrophile metabolites trigger non-enzymatic covalent modifications of highly reactive cysteine residues in KEAP1, leading to inactivate the ubiquitin ligase activity of the BCR(KEAP1) complex, promoting NFE2L2/NRF2 nuclear accumulation and expression of phase II detoxifying enzymes. In response to selective autophagy, KEAP1 is sequestered in inclusion bodies following its interaction with SQSTM1/p62, leading to inactivation of the BCR(KEAP1) complex and activation of NFE2L2/NRF2. The BCR(KEAP1) complex also mediates ubiquitination of SQSTM1/p62, increasing SQSTM1/p62 sequestering activity and degradation (By similarity). The BCR(KEAP1) complex also targets BPTF and PGAM5 for ubiquitination and degradation by the proteasome (By similarity). PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Cytoplasm;Kelch repeat;Nucleus;Reference proteome;Repeat;S-nitrosylation;Thioether bond;Ubl conjugation;Ubl conjugation pathway SUBCELLULAR LOCATION: Cytoplasm Nucleus Note=Mainly cytoplasmic. In response to selective autophagy, relocalizes to inclusion bodies following interaction with SQSTM1/p62. " P58005,PROTEIN NAMES: Sestrin-3 PROTEIN FAMILY: Sestrin family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the sestrin family. FUNCTION: May function as an intracellular leucine sensor that negatively regulates the TORC1 signaling pathway. May also regulate the insulin-receptor signaling pathway through activation of TORC2 (By similarity). This metabolic regulator may also play a role in protection against oxidative and genotoxic stresses (By similarity). May prevent the accumulation of reactive oxygen species (ROS) through the alkylhydroperoxide reductase activity born by the N-terminal domain of the protein (By similarity). KEYWORDS: Alternative splicing;Cytoplasm;Oxidoreductase;Reference proteome SUBCELLULAR LOCATION: Cytoplasm P58242,"PROTEIN NAMES: Acid sphingomyelinase-like phosphodiesterase 3b (ASM-like phosphodiesterase 3b) PROTEIN FAMILY: Acid sphingomyelinase family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the acid sphingomyelinase family. FUNCTION: Lipid-modulating phosphodiesterase. Active on the surface of macrophages and dendritic cells and strongly influences macrophage lipid composition and membrane fluidity. Acts as a negative regulator of Toll-like receptor signaling. Has in vitro phosphodiesterase activity, but the physiological substrate is unknown. Lacks activity with phosphocholine-containing lipids, but can cleave CDP-choline, and can release phosphate from ATP and ADP (in vitro). KEYWORDS: 3D-structure;Cell membrane;Disulfide bond;Glycoprotein;Glycosidase;GPI-anchor;Hydrolase;Immunity;Inflammatory response;Innate immunity;Lipid degradation;Lipid metabolism;Lipoprotein;Membrane;Metal-binding;Reference proteome;Secreted;Signal;Zinc SUBCELLULAR LOCATION: Secreted Cell membrane; Lipid-anchor, GPI-anchor " P58295,"PROTEIN NAMES: Sodium- and chloride-dependent glycine transporter 2 (GlyT-2) (GlyT2) (Solute carrier family 6 member 5) PROTEIN FAMILY: Sodium:neurotransmitter symporter (SNF) family, SLC6A5 subfamily ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A5 subfamily. FUNCTION: Sodium- and chloride-dependent glycine transporter. Terminates the action of glycine by its high affinity sodium-dependent reuptake into presynaptic terminals. May be responsible for the termination of neurotransmission at strychnine-sensitive glycinergic synapses. KEYWORDS: Alternative splicing;Cell membrane;Disulfide bond;Glycoprotein;Membrane;Metal-binding;Neurotransmitter transport;Phosphoprotein;Reference proteome;Sodium;Symport;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein " P58354,"PROTEIN NAMES: Solute carrier family 2, facilitated glucose transporter member 8 (Glucose transporter type 8) (GLUT-8) (Glucose transporter type X1) PROTEIN FAMILY: Major facilitator superfamily, Sugar transporter family, Glucose transporter subfamily ORGANISM: Bos taurus (Bovine) SIMILARITY: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily. FUNCTION: Insulin-regulated facilitative hexose transporter that mediates the transport of glucose and fructose. Facilitates hepatic influx of dietary trehalose, which in turn inhibits glucose and fructose influx triggering a starvation signal and hepatic autophagy through activation of AMPK and ULK1. Also able to mediate the transport of dehydroascorbate. KEYWORDS: Cell membrane;Cytoplasmic vesicle;Glycoprotein;Membrane;Reference proteome;Sugar transport;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Cytoplasmic vesicle membrane ; Multi-pass membrane protein Note=Principally intracellular. May move between intracellular vesicles and the plasma membrane. The dileucine internalization motif is critical for intracellular sequestration. " P58418,PROTEIN NAMES: Clarin-1 (Usher syndrome type-3 protein) PROTEIN FAMILY: Clarin family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the clarin family. FUNCTION: May have a role in the excitatory ribbon synapse junctions between hair cells and cochlear ganglion cells and presumably also in analogous synapses within the retina. KEYWORDS: Alternative splicing;Cell membrane;Deafness;Disease variant;Glycoprotein;Hearing;Membrane;Reference proteome;Retinitis pigmentosa;Sensory transduction;Transmembrane;Transmembrane helix;Usher syndrome;Vision SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein P58499,PROTEIN NAMES: Protein FAM3B (Cytokine-like protein 2-21) (Pancreatic-derived factor) (PANDER) PROTEIN FAMILY: FAM3 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the FAM3 family. FUNCTION: Induces apoptosis of alpha and beta cells in a dose- and time-dependent manner. KEYWORDS: Alternative splicing;Apoptosis;Cytokine;Direct protein sequencing;Disulfide bond;Glycoprotein;Lectin;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted. Note=Present in insulin secretory granules and likely cosecreted with insulin. Localized in discrete vesicular and perinuclear structure. P58658,PROTEIN NAMES: Protein eva-1 homolog C (Protein FAM176C) (SUE21) PROTEIN FAMILY: EVA1 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the EVA1 family. FUNCTION: Binds heparin. KEYWORDS: Alternative splicing;Glycoprotein;Lectin;Membrane;Reference proteome;Repeat;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Single-pass type I membrane protein P58735,"PROTEIN NAMES: Sulfate anion transporter 1 (SAT-1) (Solute carrier family 26 member 1) PROTEIN FAMILY: SLC26A/SulP transporter family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the SLC26A/SulP transporter (TC 2.A.53) family. FUNCTION: Sodium-independent sulfate anion transporter. Can transport other anions including bicarbonate, thiosulfate and oxalate by mediating sulfate-hydrogencarbonate, sulfate-oxalate and oxalate-hydrogencarbonate anion exchange. Mediates sulfate-thiosulfate anion exchange (By similarity). KEYWORDS: Anion exchange;Antiport;Cell membrane;Glycoprotein;Ion channel;Ion transport;Membrane;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Basolateral cell membrane ; Multi-pass membrane protein. " P58766,"PROTEIN NAMES: Phospholipase D alpha 3 (AtPLDalpha3) (PLD alpha 3) PROTEIN FAMILY: Phospholipase D family, C2-PLD subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the phospholipase D family. C2-PLD subfamily. FUNCTION: Hydrolyzes glycerol-phospholipids at the terminal phosphodiesteric bond to generate phosphatidic acids (PA). Active with phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylglycerol (PG), and phosphatidylserine (PS) as substrates. No activity toward phosphatidylinositol (PI) or PIP2. Positively mediates plant responses to hyperosmotic stresses and promotes root growth, flowering, and stress avoidance. Not involved in the abscisic acid regulation of stomatal movement and transpirational water loss. KEYWORDS: Calcium;Cytoplasm;Hydrolase;Lipid degradation;Lipid metabolism;Membrane;Metal-binding;Reference proteome;Repeat SUBCELLULAR LOCATION: Cytoplasm Membrane ; Peripheral membrane protein " P58957,"PROTEIN NAMES: Gustatory and pheromone receptor 39a, isoform C PROTEIN FAMILY: Insect chemoreceptor superfamily, Gustatory receptor (GR) family, Gr21a subfamily ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the insect chemoreceptor superfamily. Gustatory receptor (GR) family. Gr21a subfamily. FUNCTION: Gustatory receptor which mediates acceptance or avoidance behavior, depending on its substrates. Plays a role in sustaining courtship behavior in males, possibly through the reception of a stimulating arrestant pheromone. KEYWORDS: Alternative splicing;Cell membrane;Glycoprotein;Membrane;Receptor;Reference proteome;Transducer;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein " P58959,"PROTEIN NAMES: Gustatory and pheromone receptor 39a, isoform A PROTEIN FAMILY: Insect chemoreceptor superfamily, Gustatory receptor (GR) family, Gr21a subfamily ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the insect chemoreceptor superfamily. Gustatory receptor (GR) family. Gr21a subfamily. FUNCTION: Gustatory receptor which mediates acceptance or avoidance behavior, depending on its substrates. Plays a role in sustaining courtship behavior in males, possibly through the reception of a stimulating arrestant pheromone. KEYWORDS: Alternative splicing;Cell membrane;Glycoprotein;Membrane;Receptor;Reference proteome;Transducer;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein " P59015,"PROTEIN NAMES: Vacuolar protein sorting-associated protein 18 homolog PROTEIN FAMILY: VPS18 family ORGANISM: Danio rerio (Zebrafish) (Brachydanio rerio) SIMILARITY: Belongs to the VPS18 family. FUNCTION: Plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport pathways. Believed to act as a core component of the putative HOPS endosomal tethering complex which is proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion (By similarity). May be involved in vesicle trafficking to the hepatocyte apical membrane and play a role in development of the intra-hepatic biliary tree. May target endosomes to the pigment granule in melanocytes. Essential for early embryonic development. KEYWORDS: Coiled coil;Developmental protein;Endosome;Lysosome;Membrane;Metal-binding;Protein transport;Reference proteome;Transport;Zinc;Zinc-finger SUBCELLULAR LOCATION: Late endosome membrane ; Peripheral membrane protein ; Cytoplasmic side Lysosome membrane ; Peripheral membrane protein ; Cytoplasmic side Note=Cytoplasmic, peripheral membrane protein associated with early endosomes and late endosomes/lysosomes. " P59822,"PROTEIN NAMES: Interleukin-1 receptor accessory protein (IL-1 receptor accessory protein) (IL-1RAcP) PROTEIN FAMILY: Interleukin-1 receptor family ORGANISM: Macaca mulatta (Rhesus macaque) SIMILARITY: Belongs to the interleukin-1 receptor family. FUNCTION: Coreceptor for IL1RL2 in the IL-36 signaling system (By similarity). Coreceptor with IL1R1 in the IL-1 signaling system. Associates with IL1R1 bound to IL1B to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B and other pathways. Signaling involves the recruitment of adapter molecules such as TOLLIP, MYD88, and IRAK1 or IRAK2 via the respective TIR domains of the receptor/coreceptor subunits. Recruits TOLLIP to the signaling complex. Does not bind to interleukin-1 alone; binding of IL1RN to IL1R1, prevents its association with IL1R1 to form a signaling complex. The cellular response is modulated through a non-signaling association with the membrane IL1R2 decoy receptor. Coreceptor for IL1RL1 in the IL-33 signaling system (By similarity). Can bidirectionally induce pre- and postsynaptic differentiation of neurons by trans-synaptically binding to PTPRD (By similarity). May play a role in IL1B-mediated costimulation of IFNG production from T-helper 1 (Th1) cells (By similarity).; FUNCTION: [Isoform 2]: Associates with secreted ligand-bound IL1R2 and increases the affinity of secreted IL1R2 for IL1B; this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors. Enhances the ability of secreted IL1R1 to inhibit IL-33 signaling (By similarity). KEYWORDS: Alternative splicing;Cell membrane;Disulfide bond;Glycoprotein;Hydrolase;Immunoglobulin domain;Inflammatory response;Membrane;NAD;Phosphoprotein;Receptor;Reference proteome;Repeat;Secreted;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: [Isoform 1]: Cell membrane; Single-pass type I membrane protein.; SUBCELLULAR LOCATION: [Isoform 2]: Secreted. " P60008,"PROTEIN NAMES: Putative histone H1.9 (H1.9 linker histone pseudogene) (Putative spermatid-specific linker histone H1-like protein) PROTEIN FAMILY: Histone H1/H5 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the histone H1/H5 family. FUNCTION: DNA-binding protein that may be implicated in chromatin remodeling and/or transcriptional regulation during spermiogenesis, the process of spermatid maturation into spermatozoa. KEYWORDS: Chromatin regulator;Chromosome;Developmental protein;Differentiation;DNA-binding;Nucleus;Phosphoprotein;Reference proteome;Spermatogenesis;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus Chromosome " P60508,"PROTEIN NAMES: Syncytin-2 (Endogenous retrovirus group FRD member 1) (Envelope polyprotein) (HERV-FRD) (HERV-FRD_6p24.1 provirus ancestral Env polyprotein) [Cleaved into: Surface protein (SU); Transmembrane protein (TM)] PROTEIN FAMILY: Gamma type-C retroviral envelope protein family, HERV class-I FRD env subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the gamma type-C retroviral envelope protein family. HERV class-I FRD env subfamily. FUNCTION: This endogenous retroviral envelope protein has retained its original fusogenic properties and participates in trophoblast fusion and the formation of a syncytium during placenta morphogenesis. The interaction with MFSD2A is apparently important for this process.; FUNCTION: Endogenous envelope proteins may have kept, lost or modified their original function during evolution but this one can still make pseudotypes with MLV, HIV-1 or SIV-1 virions and confer infectivity. Retroviral envelope proteins mediate receptor recognition and membrane fusion during early infection. The surface protein mediates receptor recognition, while the transmembrane protein anchors the envelope heterodimer to the viral membrane through one transmembrane domain. The other hydrophobic domain, called fusion peptide, mediates fusion of the viral membrane with the target cell membrane. KEYWORDS: 3D-structure;Cell membrane;Cleavage on pair of basic residues;Disulfide bond;ERV;Glycoprotein;Membrane;Reference proteome;Signal;Transmembrane;Transmembrane helix;Transposable element;Viral envelope protein;Virion SUBCELLULAR LOCATION: Virion.; SUBCELLULAR LOCATION: [Surface protein]: Cell membrane ; Peripheral membrane protein Note=The surface protein is not anchored to the membrane, but localizes to the extracellular surface through its binding to TM.; SUBCELLULAR LOCATION: [Transmembrane protein]: Cell membrane ; Single-pass membrane protein MISCELLANEOUS: HERV-FRD subgenomic RNA has been observed.; MISCELLANEOUS: Ortholog in old-world and new-world monkeys, but not in prosimians.; MISCELLANEOUS: The human genome contains a high percentage of proviral-like elements, also called endogenous retroviruses (ERVs) that are the genomic traces of ancient infections of the germline by exogenous retroviruses. Although most of these elements are defective, some have conserved a functional envelope (env) gene, most probably diverted by the host for its benefit." P60570,"PROTEIN NAMES: Pannexin-1 [Cleaved into: Caspase-activated pannexin-1 (Caspase-activated PANX1)] PROTEIN FAMILY: Pannexin family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the pannexin family. FUNCTION: Ion channel involved in a variety of physiological functions such as blood pressure regulation, apoptotic cell clearance and oogenesis (By similarity). Forms anion-selective channels with relatively low conductance and an order of permeabilities: nitrate>iodide>chlroride>>aspartate=glutamate=gluconate (By similarity). Can release ATP upon activation through phosphorylation or cleavage at C-terminus. May play a role as a Ca(2+)-leak channel to regulate ER Ca(2+) homeostasis (By similarity).; FUNCTION: [Caspase-activated pannexin-1]: During apoptosis, the C terminal tail is cleaved by caspases, which opens the main pore acting as a large-pore ATP efflux channel with a broad distribution, which allows the regulated release of molecules and ions smaller than 1 kDa, such as nucleotides ATP and UTP, and selective plasma membrane permeability to attract phagocytes that engulf the dying cells. KEYWORDS: Calcium;Calcium channel;Calcium transport;Cell membrane;Disulfide bond;Endoplasmic reticulum;Glycoprotein;Ion channel;Ion transport;Membrane;Phosphoprotein;Reference proteome;S-nitrosylation;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Endoplasmic reticulum membrane ; Multi-pass membrane protein " P60762,"PROTEIN NAMES: Mortality factor 4-like protein 1 (MORF-related gene 15 protein) (Testis-expressed gene 189 protein) (Transcription factor-like protein MRG15) ORGANISM: Mus musculus (Mouse) FUNCTION: Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when directly recruited to sites of DNA damage. As part of the SIN3B complex represses transcription and counteracts the histone acetyltransferase activity of EP300 through the recognition H3K27ac marks by PHF12 and the activity of the histone deacetylase HDAC2. SIN3B complex is recruited downstream of the constitutively active genes transcriptional start sites through interaction with histones and mitigates histone acetylation and RNA polymerase II progression within transcribed regions contributing to the regulation of transcription. Required for homologous recombination repair (HRR) and resistance to mitomycin C (MMC). Involved in the localization of PALB2, BRCA2 and RAD51, but not BRCA1, to DNA-damage foci. KEYWORDS: Acetylation;Alternative splicing;Chromatin regulator;DNA damage;DNA recombination;DNA repair;Growth regulation;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " P61085,"PROTEIN NAMES: Ubiquitin-conjugating enzyme E2 K (E2 ubiquitin-conjugating enzyme K) (Huntingtin-interacting protein 2) (HIP-2) (Ubiquitin carrier protein) (Ubiquitin-conjugating enzyme E2-25 kDa) (Ubiquitin-conjugating enzyme E2(25K)) (Ubiquitin-conjugating enzyme E2-25K) (Ubiquitin-protein ligase) PROTEIN FAMILY: Ubiquitin-conjugating enzyme family ORGANISM: Bos taurus (Bovine) SIMILARITY: Belongs to the ubiquitin-conjugating enzyme family. FUNCTION: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro, in the presence or in the absence of BRCA1-BARD1 E3 ubiquitin-protein ligase complex, catalyzes the synthesis of 'Lys-48'-linked polyubiquitin chains. Does not transfer ubiquitin directly to but elongates monoubiquitinated substrate protein. Mediates the selective degradation of short-lived and abnormal proteins, such as the endoplasmic reticulum-associated degradation (ERAD) of misfolded lumenal proteins. Ubiquitinates huntingtin. May mediate foam cell formation by the suppression of apoptosis of lipid-bearing macrophages through ubiquitination and subsequence degradation of p53/TP53 (By similarity). Proposed to be involved in ubiquitination and proteolytic processing of NF-kappa-B; in vitro supports ubiquitination of NFKB1. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: 3D-structure;Acetylation;ATP-binding;Cytoplasm;Isopeptide bond;Nucleotide-binding;Phosphoprotein;Reference proteome;Transferase;Ubl conjugation;Ubl conjugation pathway SUBCELLULAR LOCATION: Cytoplasm. " P61647,"PROTEIN NAMES: Alpha-2,8-sialyltransferase 8F (Sialyltransferase 8F) (SIAT8-F) (Sialyltransferase St8Sia VI) (ST8SiaVI) PROTEIN FAMILY: Glycosyltransferase 29 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the glycosyltransferase 29 family. FUNCTION: Alpha-2,8-sialyltransferase that prefers O-glycans to N-glycans or glycolipids as acceptor substrates. The minimal acceptor substrate is the NeuAc-alpha-2,3(6)-Gal sequence at the non-reducing end of their carbohydrate groups. PATHWAY: Protein modification; protein glycosylation. KEYWORDS: Disulfide bond;Glycoprotein;Glycosyltransferase;Golgi apparatus;Lipid metabolism;Membrane;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus membrane ; Single-pass type II membrane protein " P61869,"PROTEIN NAMES: Secreted mono- and diacylglycerol lipase A (MDGL) PROTEIN FAMILY: AB hydrolase superfamily, Lipase family, Class 3 subfamily ORGANISM: Penicillium cyclopium SIMILARITY: Belongs to the AB hydrolase superfamily. Lipase family. Class 3 subfamily. FUNCTION: Secreted lipase strictly specific to mono- and diacylglycerol, but not triacylglycerol. Shows the highest activity on 1,2-dibutyrin substrate. KEYWORDS: 3D-structure;Disulfide bond;Glycoprotein;Hydrolase;Lipid degradation;Lipid metabolism;Metal-binding;Secreted;Signal;Zinc;Zymogen SUBCELLULAR LOCATION: Secreted " P62598,"PROTEIN NAMES: Two-component response regulator ARR12 PROTEIN FAMILY: ARR family, Type-B subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the ARR family. Type-B subfamily. FUNCTION: Transcriptional activator that binds specifically to the DNA sequence 5'-[AG]GATT-3'. Functions as a response regulator involved in His-to-Asp phosphorelay signal transduction system. Phosphorylation of the Asp residue in the receiver domain activates the ability of the protein to promote the transcription of target genes. Could directly activate some type-A response regulators in response to cytokinins. Involved in the root-meristem size determination through the regulation of cell differentiation. Involved in activating SHY2 during meristem growth and controls PIN expression via activation of SHY2. KEYWORDS: Activator;Cytokinin signaling pathway;DNA-binding;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation;Two-component regulatory system SUBCELLULAR LOCATION: Nucleus. " P62995,"PROTEIN NAMES: Transformer-2 protein homolog beta (TRA-2 beta) (TRA2-beta) (hTRA2-beta) (Splicing factor, arginine/serine-rich 10) (Transformer-2 protein homolog B) PROTEIN FAMILY: Splicing factor SR family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the splicing factor SR family. FUNCTION: Sequence-specific RNA-binding protein which participates in the control of pre-mRNA splicing. Can either activate or suppress exon inclusion. Acts additively with RBMX to promote exon 7 inclusion of the survival motor neuron SMN2. Activates the splicing of MAPT/Tau exon 10. Alters pre-mRNA splicing patterns by antagonizing the effects of splicing regulators, like RBMX. Binds to the AG-rich SE2 domain in the SMN exon 7 RNA. Binds to pre-mRNA. KEYWORDS: 3D-structure;Acetylation;Activator;Alternative splicing;Direct protein sequencing;Isopeptide bond;Methylation;mRNA processing;mRNA splicing;Nucleus;Phosphoprotein;Reference proteome;Repressor;RNA-binding;Ubl conjugation SUBCELLULAR LOCATION: Nucleus " P63883,"PROTEIN NAMES: N-acetylmuramoyl-L-alanine amidase AmiC PROTEIN FAMILY: N-acetylmuramoyl-L-alanine amidase 3 family ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family. FUNCTION: Cell-wall hydrolase involved in septum cleavage during cell division. Can also act as powerful autolysin in the presence of murein synthesis inhibitors. KEYWORDS: 3D-structure;Cell wall biogenesis/degradation;Hydrolase;Periplasm;Reference proteome;Signal SUBCELLULAR LOCATION: Periplasm Note=Present throughout the periplasm in non-dividing cells, but localizes almost exclusively to a ring at the site of constriction in dividing cells. " P67095,"PROTEIN NAMES: Phosphodiesterase YfcE PROTEIN FAMILY: Metallophosphoesterase superfamily, YfcE family ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the metallophosphoesterase superfamily. YfcE family. FUNCTION: Shows phosphodiesterase activity. Shows significant activity toward bis-p-nitrophenyl phosphate (bis-pNPP), an artificial substrate commonly used to detect phosphodiesterase activity. Also hydrolyzes, with lower efficiency, two other artificial phosphodiesterase substrates, thymidine 5'-monophosphate p-nitrophenyl ester (pNP-TMP) and p-nitrophenylphosphorylcholine (pNPPC). Shows phosphomonoesterase activity toward p-nitrophenyl phosphate (pNPP), with much lower catalytic efficiency. No activity was detected against a wide variety of naturally occurring phosphomonoesters and phosphodiesters, including various 2',3'- and 3',5'-cyclic nucleotides, suggesting that YfcE is highly specific for its physiological substrate, which is not yet known. KEYWORDS: 3D-structure;Hydrolase;Manganese;Metal-binding;Reference proteome " P67444,"PROTEIN NAMES: Xanthine permease XanQ PROTEIN FAMILY: Nucleobase:cation symporter-2 (NCS2) family ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) family. FUNCTION: Specific, proton motive force-dependent high-affinity transporter for xanthine. KEYWORDS: Cell inner membrane;Cell membrane;Membrane;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell inner membrane ; Multi-pass membrane protein " P68515,"PROTEIN NAMES: Bradykinin-potentiating and C-type natriuretic peptides (Angiotensin-converting enzyme inhibitor) (BPP-CNP homolog) [Cleaved into: Bradykinin-potentiating peptide 13a (BPP-13a) (Bradykinin-potentiating peptide S3,1); Bradykinin-potentiating peptide 10c (BPP-10c) (BPP-2) (Bradykinin-potentiating peptide S4,3,1); Bradykinin-potentiating peptide 12b (BPP-12b) (Bradykinin-potentiating peptide S4,3,2); Bradykinin-potentiating peptide 11e (BPP-11e); Bradykinin-potentiating peptide 5a (BPP-5a) (Bradykinin-potentiating peptide S5,2) (Bradykinin-potentiating peptide Va) (BPPVa) (Proline-rich peptide 5a) (PRO-5a); C-type natriuretic peptide (CNP)] PROTEIN FAMILY: Bradykinin-potentiating peptide family; Natriuretic peptide family ORGANISM: Bothrops insularis (Golden lancehead) (Lachesis insularis) SIMILARITY: In the N-terminal section; belongs to the bradykinin-potentiating peptide family.; SIMILARITY: In the C-terminal section; belongs to the natriuretic peptide family. FUNCTION: [Bradykinin-potentiating peptide 5a]: Modestly inhibits ACE (with highest affinity for the N-site) and reveals strong bradykinin-potentiating activity. Induces nitric oxide (NO) production depended on muscarinic acetylcholine receptor M1 subtype (CHRM1) and bradykinin B2 receptor (BDKRB2) activation. Both these receptors contribute to the vasodilation induced by this peptide that may have an indirect action on BDKRB2 and a direct agonistic action on CHRM1.; FUNCTION: [Bradykinin-potentiating peptide 10c]: Peptide with several activities. It inhibits the activity of the angiotensin-converting enzyme (ACE) by a preferential interaction with its C-domain. It evokes transient hypotension (-14 mmHg) similar to that evoked by 0.5 ug of bradykinin, when injected alone into rats. It has a high bradykinin-potentiating effect (120%), when 60 nmol of BPP-10c are coinjected with 0.5 ug of bradykinin into rats. Does not affect angiotensin-1 pressor effects. Shows potent and long-lasting antihypertensive activity as well as a reduction of the heart rate. It also binds and dose-dependently promotes the activation of cytosolic argininosuccinate synthase (ASS1), an enzyme that catalyzes the conversion of citrulline, L-aspartate and ATP to argininosuccinate, AMP and pyrophosphate. It also enhances ASS1-dependent arginine production in HEK 293 cells, as well as in spontaneous hypertensive rat (SHR) and Wistar rat plasma. In addition, it induces the production of nitric-oxide (NO) by HUVEC cells via the endothelial nitric-oxide synthase (NOS3), which use arginine as a substrate and produce NO. It has been shown to be internalized by ASS1-expressing endothelial (HUVEC) and kidney (HEK 293) cells, and is detected homogenously distributed within the cell cytoplasm for up to 2 hours.; FUNCTION: [C-type natriuretic peptide]: has a vasorelaxant activity in rat aortic strips and a diuretic potency in anesthetized rats (By similarity). May act by activating natriuretic receptors (NPR1 and/or NPR2). KEYWORDS: Cleavage on pair of basic residues;Cytoplasm;Direct protein sequencing;Disulfide bond;G-protein coupled acetylcholine receptor impairing toxin;G-protein coupled receptor impairing toxin;Hypotensive agent;Metalloenzyme inhibitor;Metalloprotease inhibitor;Protease inhibitor;Pyrrolidone carboxylic acid;Repeat;Secreted;Signal;Toxin;Vasoactive;Vasodilator SUBCELLULAR LOCATION: Secreted Cytoplasm, cytosol. Note=BPP-10c is internalized in the cytosol of prey cells. " P68827,"PROTEIN NAMES: N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase (Mannose 6-phosphate-uncovering enzyme) (Phosphodiester alpha-GlcNAcase) ORGANISM: Bos taurus (Bovine) FUNCTION: Catalyzes the second step in the formation of the mannose 6-phosphate targeting signal on lysosomal enzyme oligosaccharides by removing GlcNAc residues from GlcNAc-alpha-P-mannose moieties, which are formed in the first step. Also hydrolyzes UDP-GlcNAc, a sugar donor for Golgi N-acetylglucosaminyltransferases. PATHWAY: Protein modification; protein glycosylation. KEYWORDS: Direct protein sequencing;Disulfide bond;EGF-like domain;Glycoprotein;Golgi apparatus;Hydrolase;Membrane;Reference proteome;Sialic acid;Signal;Transmembrane;Transmembrane helix;Zymogen SUBCELLULAR LOCATION: Golgi apparatus, Golgi stack membrane ; Single-pass type I membrane protein Golgi apparatus, trans-Golgi network Note=Cis/medial Golgi. " P69478,"PROTEIN NAMES: Carbohydrate sulfotransferase 11 (Chondroitin 4-O-sulfotransferase 1) (Chondroitin 4-sulfotransferase 1) (C4S-1) (C4ST-1) (C4ST1) PROTEIN FAMILY: Sulfotransferase 2 family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the sulfotransferase 2 family. FUNCTION: Catalyzes the transfer of sulfate to position 4 of the N-acetylgalactosamine (GalNAc) residue of chondroitin. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage and is distributed on the surfaces of many cells and extracellular matrices. Can also sulfate Gal residues in desulfated dermatan sulfate. Preferentially sulfates in GlcA->GalNAc unit than in IdoA->GalNAc unit. Does not form 4, 6-di-O-sulfated GalNAc when chondroitin sulfate C is used as an acceptor (By similarity). KEYWORDS: Carbohydrate metabolism;Direct protein sequencing;Glycoprotein;Golgi apparatus;Membrane;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus membrane ; Single-pass type II membrane protein " P69834,"PROTEIN NAMES: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, chloroplastic (4-diphosphocytidyl-2C-methyl-D-erythritol synthase) (MEP cytidylyltransferase) (AtMECT) (AtMEPCT) PROTEIN FAMILY: IspD/TarI cytidylyltransferase family, IspD subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the IspD/TarI cytidylyltransferase family. IspD subfamily. FUNCTION: Enzyme of the plastid non-mevalonate pathway for isoprenoid biosynthesis that catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP). Is essential for chloroplast development and required for pigments and gibberellins biosynthesis. PATHWAY: Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6. KEYWORDS: 3D-structure;Acetylation;Chloroplast;Isoprene biosynthesis;Nucleotidyltransferase;Plastid;Reference proteome;Transferase;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast stroma " P69929,PROTEIN NAMES: Delta-actitoxin-Amc1a (Delta-AITX-Amc1a) (AnmTX Ama 9a-1) (Peptide toxins Am I) (Peptide toxins Am-1) PROTEIN FAMILY: Sea anemone structural class 9a family ORGANISM: Antheopsis maculata (Sea anemone) SIMILARITY: Belongs to the sea anemone structural class 9a family. FUNCTION: May inhibit voltage-gated sodium channels (Nav). KEYWORDS: Cleavage on pair of basic residues;Disulfide bond;Hydroxylation;Ion channel impairing toxin;Nematocyst;Repeat;Secreted;Signal;Toxin;Voltage-gated sodium channel impairing toxin SUBCELLULAR LOCATION: Secreted Nematocyst P70056,"PROTEIN NAMES: Forkhead box protein H1 (Forkhead activin signal transducer 1) (Fast-1) (xFAST-1) (XFoxH1a) ORGANISM: Xenopus laevis (African clawed frog) FUNCTION: Transcriptional activator. Recognizes and binds to the DNA sequence 5'-TGT[GT][GT]ATT-3'. Upon TGF-beta induction, forms a transcriptionally active complex with smad2 and smad4 called activin-responsive factor 1 (ARF1), which binds a site on the mix-B/mix.2 promoter called the activin response element (ARE). Binds to activated smads and the ARE with much lower affinity than fast3. Necessary for the first steps in mesoderm specification, directly inducing mesodermal genes. Acts with fast3 to control the convergent extension movements of gastrulation. Binds to the proximal element (PE) of the gsc gene and cooperates with gtf2ird1/wbscr11 and SMAD proteins to regulate gsc transcription. KEYWORDS: 3D-structure;Activator;Developmental protein;DNA-binding;Gastrulation;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " P70186,"PROTEIN NAMES: Epiphycan (Dermatan sulfate proteoglycan 3) (Proteoglycan-Lb) (PG-Lb) (Small chondroitin/dermatan sulfate proteoglycan) PROTEIN FAMILY: Small leucine-rich proteoglycan (SLRP) family, SLRP class III subfamily ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class III subfamily. FUNCTION: May have a role in bone formation and also in establishing the ordered structure of cartilage through matrix organization. KEYWORDS: Disulfide bond;Extracellular matrix;Glycoprotein;Leucine-rich repeat;Proteoglycan;Reference proteome;Repeat;Secreted;Signal SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix. Note=Surrounding resting, proliferating, and hypertrophic chondrocytes. " P70245,"PROTEIN NAMES: 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase (Cholestenol Delta-isomerase) (Delta(8)-Delta(7) sterol isomerase) (D8-D7 sterol isomerase) (Emopamil-binding protein) PROTEIN FAMILY: EBP family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the EBP family. FUNCTION: Catalyzes the conversion of Delta(8)-sterols to their corresponding Delta(7)-isomers. PATHWAY: Steroid biosynthesis; cholesterol biosynthesis. KEYWORDS: Acetylation;Cholesterol biosynthesis;Cholesterol metabolism;Cytoplasmic vesicle;Direct protein sequencing;Disease variant;Endoplasmic reticulum;Isomerase;Lipid biosynthesis;Lipid metabolism;Membrane;Nucleus;Reference proteome;Steroid biosynthesis;Steroid metabolism;Sterol biosynthesis;Sterol metabolism;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein Nucleus envelope Cytoplasmic vesicle Note=During interphase, detected on the endoplasmic reticulum and the nuclear envelope. During mitosis, detected on cytoplasmic vesicles. MISCELLANEOUS: Binds to the phenylalkylamine calcium-ion antagonist emopamil, an anti-ischemic drug." P70310,"PROTEIN NAMES: Neurotensin receptor type 2 (NT-R-2) (NTR2) (Low-affinity levocabastine-sensitive neurotensin receptor) (NTRL) PROTEIN FAMILY: G-protein coupled receptor 1 family, Neurotensin receptor subfamily, NTSR2 sub-subfamily ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the G-protein coupled receptor 1 family. Neurotensin receptor subfamily. NTSR2 sub-subfamily. FUNCTION: Receptor for the tridecapeptide neurotensin. It is associated with G proteins that activate a phosphatidylinositol-calcium second messenger system. KEYWORDS: Cell membrane;Disulfide bond;G-protein coupled receptor;Lipoprotein;Membrane;Palmitate;Phosphoprotein;Receptor;Reference proteome;Transducer;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. " P70323,"PROTEIN NAMES: T-box transcription factor TBX1 (T-box protein 1) (Testis-specific T-box protein) ORGANISM: Mus musculus (Mouse) FUNCTION: Transcription factor that plays a key role in cardiovascular development by promoting pharyngeal arch segmentation during embryonic development. Also involved in craniofacial muscle development. Together with NKX2-5, acts as a regulator of asymmetric cardiac morphogenesis by promoting expression of PITX2. Acts upstream of TBX1 for the formation of the thymus and parathyroid glands from the third pharyngeal pouch. Required for hair follicle stem cell self-renewal. Binds to the palindromic T site 5'-TTCACACCTAGGTGTGAA-3' DNA sequence (By similarity). KEYWORDS: Alternative splicing;Developmental protein;DNA-binding;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " P70348,PROTEIN NAMES: Chorion-specific transcription factor GCMa (GCM motif protein 1) (mGCM1) (mGCMa) (Glial cells missing homolog 1) ORGANISM: Mus musculus (Mouse) FUNCTION: Transcription factor that is necessary for placental development. Involved in the control of expression of placental growth factor (PGF) and other placenta-specific genes. Binds to the trophoblast-specific element 2 (TSE2) of the aromatase gene enhancer. Binds to the SYDE1 promoter. Has a central role in mediating the differentiation of trophoblast cells along both the villous and extravillous pathways in placental development (By similarity). KEYWORDS: 3D-structure;Developmental protein;DNA-binding;Metal-binding;Nucleus;Reference proteome;Transcription;Transcription regulation;Ubl conjugation;Zinc SUBCELLULAR LOCATION: Nucleus P70379,PROTEIN NAMES: Fibroblast growth factor 14 (FGF-14) (Fibroblast growth factor homologous factor 4) (FHF-4) PROTEIN FAMILY: Heparin-binding growth factors family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the heparin-binding growth factors family. FUNCTION: Probably involved in nervous system development and function. KEYWORDS: Alternative splicing;Growth factor;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus P70452,"PROTEIN NAMES: Syntaxin-4 PROTEIN FAMILY: Syntaxin family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the syntaxin family. FUNCTION: Plasma membrane t-SNARE that mediates docking of transport vesicles. Necessary for the translocation of SLC2A4 from intracellular vesicles to the plasma membrane. In neurons, recruited at neurite tips to membrane domains rich in the phospholipid 1-oleoyl-2-palmitoyl-PC (OPPC) which promotes neurite tip surface expression of the dopamine transporter SLC6A3/DAT by facilitating fusion of SLC6A3-containing transport vesicles with the plasma membrane (By similarity). Together with STXB3 and VAMP2, may also play a role in docking/fusion of intracellular GLUT4-containing vesicles with the cell surface in adipocytes and in docking of synaptic vesicles at presynaptic active zones. KEYWORDS: 3D-structure;Cell membrane;Cell projection;Coiled coil;Membrane;Neurotransmitter transport;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane ; Single-pass type IV membrane protein Cell projection, neuron projection Note=Localizes to neurite tips in neuronal cells. " P70459,"PROTEIN NAMES: ETS domain-containing transcription factor ERF PROTEIN FAMILY: ETS family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the ETS family. FUNCTION: Potent transcriptional repressor that binds to the H1 element of the Ets2 promoter. May regulate other genes involved in cellular proliferation (By similarity). Required for extraembryonic ectoderm differentiation, ectoplacental cone cavity closure, and chorioallantoic attachment. May be important for regulating trophoblast stem cell differentiation. KEYWORDS: DNA-binding;Isopeptide bond;Nucleus;Phosphoprotein;Reference proteome;Repressor;Transcription;Transcription regulation;Ubl conjugation SUBCELLULAR LOCATION: Nucleus. " P70658,"PROTEIN NAMES: C-X-C chemokine receptor type 4 (CXC-R4) (CXCR-4) (Fusin) (Leukocyte-derived seven transmembrane domain receptor) (LESTR) (Pre-B-cell-derived chemokine receptor) (PB-CKR) (Stromal cell-derived factor 1 receptor) (SDF-1 receptor) (CD antigen CD184) PROTEIN FAMILY: G-protein coupled receptor 1 family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the G-protein coupled receptor 1 family. FUNCTION: Receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ion levels and enhancing MAPK1/MAPK3 activation. Involved in the AKT signaling cascade (By similarity). Plays a role in regulation of cell migration, e.g. during wound healing. Acts as a receptor for extracellular ubiquitin; leading to enhanced intracellular calcium ions and reduced cellular cAMP levels. Binds bacterial lipopolysaccharide (LPS) et mediates LPS-induced inflammatory response, including TNF secretion by monocytes (By similarity). Involved in hematopoiesis and in cardiac ventricular septum formation. Also plays an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells. Involved in cerebellar development. In the CNS, could mediate hippocampal-neuron survival. KEYWORDS: Alternative splicing;Cell junction;Cell membrane;Disulfide bond;Endosome;G-protein coupled receptor;Glycoprotein;Isopeptide bond;Lysosome;Membrane;Phosphoprotein;Proteoglycan;Receptor;Reference proteome;Sulfation;Transducer;Transmembrane;Transmembrane helix;Ubl conjugation SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Cell junction Early endosome Late endosome Lysosome Note=In unstimulated cells, diffuse pattern on plasma membrane. On agonist stimulation, colocalizes with ITCH at the plasma membrane where it becomes ubiquitinated (By similarity). In the presence of antigen, distributes to the immunological synapse forming at the T-cell-APC contact area, where it localizes at the peripheral and distal supramolecular activation cluster (SMAC) (By similarity). " P70669,"PROTEIN NAMES: Phosphate-regulating neutral endopeptidase PHEX (Metalloendopeptidase homolog PEX) (Phosphate regulating neutral endopeptidase) (Vitamin D-resistant hypophosphatemic rickets protein) (X-linked hypophosphatemia protein) (HYP) PROTEIN FAMILY: Peptidase M13 family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the peptidase M13 family. FUNCTION: Peptidase that cleaves SIBLING (small integrin-binding ligand, N-linked glycoprotein)-derived ASARM peptides, thus regulating their biological activity (By similarity). Cleaves ASARM peptides between Ser and Glu or Asp residues (By similarity). Regulates osteogenic cell differentiation and bone mineralization through the cleavage of the MEPE-derived ASARM peptide. Promotes dentin mineralization and renal phosphate reabsorption by cleaving DMP1- and MEPE-derived ASARM peptides. Inhibits the cleavage of MEPE by CTSB/cathepsin B thus preventing MEPE degradation (By similarity). KEYWORDS: Biomineralization;Cell membrane;Disulfide bond;Glycoprotein;Hydrolase;Membrane;Metal-binding;Metalloprotease;Protease;Reference proteome;Signal-anchor;Transmembrane;Transmembrane helix;Zinc SUBCELLULAR LOCATION: Cell membrane ; Single-pass type II membrane protein " P70699,"PROTEIN NAMES: Lysosomal alpha-glucosidase (Acid maltase) PROTEIN FAMILY: Glycosyl hydrolase 31 family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the glycosyl hydrolase 31 family. FUNCTION: Essential for the degradation of glycogen in lysosomes. Has highest activity on alpha-1,4-linked glycosidic linkages, but can also hydrolyze alpha-1,6-linked glucans. KEYWORDS: Direct protein sequencing;Disulfide bond;Glycoprotein;Glycosidase;Hydrolase;Lysosome;Membrane;Reference proteome;Signal SUBCELLULAR LOCATION: Lysosome Lysosome membrane " P71629,"PROTEIN NAMES: CRISPR system single-strand-specific deoxyribonuclease Cas10/Csm1 (subtype III-A) (ssDNase Cas10) (Cyclic oligoadenylate synthase) PROTEIN FAMILY: CRISPR-associated Cas10/Csm1 family ORGANISM: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) SIMILARITY: Belongs to the CRISPR-associated Cas10/Csm1 family. FUNCTION: CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). The type III-A Csm effector complex binds crRNA and acts as a crRNA-guided RNase, DNase and cyclic oligoadenylate synthase; binding of target RNA cognate to the crRNA is required for all activities (Probable). This CRISPR-Cas system protects bacteria against transformation with plasmids containing DNA homologous to its spacer regions.; FUNCTION: This subunit is a single-strand-specific deoxyribonuclease (ssDNase) which digests both linear and circular ssDNA; it has both exo- and endonuclease activity.; FUNCTION: ssDNase activity is stimulated in the ternary Csm effector complex; binding of cognate target RNA activates the ssDNase, as the target RNA is degraded ssDNA activity decreases.; FUNCTION: When associated with the ternary Csm effector complex (the crRNA, Cas proteins and a cognate target ssRNA) synthesizes cyclic oligoadenylates (cOA) from ATP. cOAs are second messengers that stimulate the ssRNase activity of Csm6, inducing an antiviral state important for defense against invading nucleic acids. KEYWORDS: 3D-structure;Antiviral defense;ATP-binding;Endonuclease;Exonuclease;Hydrolase;Nuclease;Nucleotide-binding;Reference proteome;RNA-binding;Transferase MISCELLANEOUS: Encoded in a type III-A CRISPR locus." P71778,"PROTEIN NAMES: Esterase/beta-lactamase LipL PROTEIN FAMILY: Beta-lactamase family ORGANISM: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) SIMILARITY: Belongs to the beta-lactamase family. FUNCTION: Shows both esterase and beta-lactamase activities, with a much higher activity against phenyl esters than against beta-lactams. Shows esterase activity against both long-chain and short-chain p-nitrophenol (pNP) esters, with a preference for shorter chain esters. Hydrolyzes substrates containing beta-lactam ring such as nitrocefin and ampicillin. Functions as an immunogen that activates both humoral and cell-mediated responses. KEYWORDS: Cell membrane;Cell wall;Hydrolase;Membrane;Reference proteome;Secreted SUBCELLULAR LOCATION: Secreted, cell wall Cell membrane " P71858,"PROTEIN NAMES: Acyl-CoA dehydrogenase FadE29 (ACAD) (3-oxo-23,24-bisnorchol-4-en-22-oyl-CoA dehydrogenase beta subunit) (3-oxo-4-pregnene-20-carboxyl-CoA dehydrogenase beta subunit) PROTEIN FAMILY: Acyl-CoA dehydrogenase family ORGANISM: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) SIMILARITY: Belongs to the acyl-CoA dehydrogenase family. FUNCTION: Involved in the third cycle of side chain dehydrogenation in the beta-oxidation of cholesterol catabolism. Contributes partly to the virulence by increasing the efficiency of beta-oxidation. Catalyzes the dehydrogenation of 2'-propanoyl-CoA ester side chains of 3-oxo-4-pregnene-20-carboxyl-CoA (3-OPC-CoA) to yield 3-oxo-4,17-pregnadiene-20-carboxyl-CoA (3-OPDC-CoA). Also able to dehydrogenate steroyl-CoA such as 3-oxo-chol-4-en-24-oyl-CoA (3-OCO-CoA), 1beta-(2'-propanoyl-CoA)-3a-alpha-H- 7a-beta-methylhexahydro-4-indanone (indanone-CoA ester), hexahydroindanone and pregenenone. PATHWAY: Steroid metabolism; cholesterol degradation. KEYWORDS: Cholesterol metabolism;FAD;Flavoprotein;Lipid degradation;Lipid metabolism;NAD;NADP;Oxidoreductase;Reference proteome;Steroid metabolism;Sterol metabolism;Virulence " P72131,"PROTEIN NAMES: HTH-type transcriptional regulator PtxR (Pseudomonas exotoxin A regulator) PROTEIN FAMILY: LysR transcriptional regulatory family ORGANISM: Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) SIMILARITY: Belongs to the LysR transcriptional regulatory family. FUNCTION: Plays an important role in the regulation of the production of the virulence factor exotoxin A (toxA), via positive regulation of the transcription of the toxA gene. Acts by binding directly to the toxA promoter region. Besides toxA, PtxR modulates the expression of genes that code for the QS-controlled virulence factors. It negatively regulates the expression of the rhamnolipid and pyocyanine genes, through the autoinducer synthase RhlI, and the PQS synthesis operon pqsABCDE, while it positively regulates the expression of lasB through the autoinducer synthase LasI. Also positively regulates the expression of the exotoxin A regulatory protein (toxR or regA).; FUNCTION: In addition, is involved in the positive regulation of glucose metabolism via the regulation of the expression of the kgu and gad operons. Acts by binding directly to the promoter region of the kgu and gad operons. KEYWORDS: Activator;DNA-binding;Reference proteome;Repressor;Transcription;Transcription regulation " P72758,"PROTEIN NAMES: Carboxysome assembly protein CcmM (CcmM73) (M73) (Carbon dioxide concentrating mechanism protein CcmM) PROTEIN FAMILY: Gamma-class carbonic anhydrase family ORGANISM: Synechocystis sp. (strain PCC 6803 / Kazusa) SIMILARITY: Belongs to the gamma-class carbonic anhydrase family. FUNCTION: Functions as a scaffold protein for the assembly of beta-carboxysomes, initiates carboxysome assembly via its N-terminal domain binding to CcaA, CcmK and CcmL. Binds HCO(3)-, suggesting it may play a role in the activity or regulation of bicarbonate dehydration. Also initiates carboxysome assembly by coalescing RuBisCO (ribulose bisphosphate carboxylase, rbcL-rbcS) via its SSU-like domains. Produced as a full-length and a shorter form; both forms are required for correct carboxysome assembly and growth (By similarity). Despite its strong similarity to gamma-class carbonic anhydrase (CA) it does not have detectable CA activity.; FUNCTION: Beta-carboxysome assembly initiates when soluble RuBisCO is condensed into a liquid matrix in a pre-carboxysome by the RbcS-like domains of probably both forms of CcmM. CcmN interacts with the N-terminus of full length CcmM, and then recruits the shell proteins (CcmK) via CcmN's encapsulation peptide. CcmM73 also interacts with CcmK proteins and CcmL directly. Shell formation requires CcmK proteins and CcmO. CcmL caps the otherwise elongated carboxysome. Once fully encapsulated carboxysomes are formed, they migrate within the cell probably via interactions with the cytoskeleton. KEYWORDS: Alternative initiation;Bacterial microcompartment;Carbon dioxide fixation;Carboxysome;Photosynthesis;Reference proteome;Repeat SUBCELLULAR LOCATION: Carboxysome Note=This cyanobacterium makes beta-type carboxysomes. Full length protein associates with the shell portion of carboxysomes, CcmM52 associates with both the soluble and shell portion of carboxysomes. " P73276,"PROTEIN NAMES: Sensor histidine kinase Hik2 (CSK) PROTEIN FAMILY: Chloroplast sensor kinase protein family ORGANISM: Synechocystis sp. (strain PCC 6803 / Kazusa) SIMILARITY: Belongs to the chloroplast sensor kinase protein family. FUNCTION: Member of possibly 2 two-component regulatory system(s) Hik2/Rre1 and Hik2/RppA. Transduces PQ (plastoquinone) redox signals to photosystem gene expression machinery during the adjustment of photosystem stoichiometry. Reduced PQ suppresses its autophosphorylation activity (i.e. kinase activity is higher under oxidizing conditions) (Probable). Member of two-component regulatory system Hik2/Rre1, controls expression of sigB (sll0306), sll0528, slr1119, slr0852 and ssr3188 in response to hyperosmotic stress (Probable). Activity responds to high salt (with a linear response as concentrations rise to 0.5 M NaCl); detects Cl(-) levels. Autophosphorylates and transfers phosphate to Rre1. May transfer phosphate to RppA in a possible Hik2/RppA two-component system (Probable). KEYWORDS: 3Fe-4S;Cytoplasm;Iron;Iron-sulfur;Kinase;Metal-binding;Phosphoprotein;Reference proteome;Transferase;Two-component regulatory system SUBCELLULAR LOCATION: Cytoplasm " P74102,"PROTEIN NAMES: Orange carotenoid-binding protein (OCP) [Cleaved into: Red carotenoid-binding protein (RCP)] PROTEIN FAMILY: Orange carotenoid-binding protein family ORGANISM: Synechocystis sp. (strain PCC 6803 / Kazusa) SIMILARITY: Belongs to the orange carotenoid-binding protein family. FUNCTION: Acts as a blue-light photoreceptor and photo-protectant. Essential for inhibiting damaged induced by excess blue-green light via a process known as non-photochemical quenching (NPQ). In the dark or dim light the stable inactive form (OCP-O) is orange, upon illumination with blue-green light it converts to a metastable active red form (OCP-R), inducing energy dissipation, quenching cellular fluorescence via NPQ. One OCP-R molecule is sufficient to quench 1 phycobilisome. More OCP-R accumulates under high-light and low temperature; in the dark OCP-R spontaneously reverts to OCP-O. Reversion of OCP-O is accelerated by FRP. A kinetic study suggests conversion of OCP-O to OCP-R is limited by cis-trans proline isomerization of either Gln224-Pro225 or Pro225-Pro226. KEYWORDS: 3D-structure;Antenna complex;Chromophore;Direct protein sequencing;Membrane;Photoreceptor protein;Phycobilisome;Receptor;Reference proteome;Sensory transduction;Thylakoid SUBCELLULAR LOCATION: Cellular thylakoid membrane ; Peripheral membrane protein ; Cytoplasmic side Note=Associated with the phycobilisome on the cytoplasmic side of the thylakoid membrane. " P74383,PROTEIN NAMES: Isoaspartyl peptidase/L-asparaginase (Beta-aspartyl-peptidase) (Isoaspartyl dipeptidase) [Cleaved into: Isoaspartyl peptidase/L-asparaginase subunit alpha; Isoaspartyl peptidase/L-asparaginase subunit beta] PROTEIN FAMILY: Ntn-hydrolase family ORGANISM: Synechocystis sp. (strain PCC 6803 / Kazusa) SIMILARITY: Belongs to the Ntn-hydrolase family. FUNCTION: Degrades proteins damaged by L-isoaspartyl residue formation (also known as beta-Asp residues). Probably performs the final step in the degradation of the reserve polymer cyanophycin (depolymerizes the building block L-beta-Asp-Arg). Also has L-asparaginase activity. KEYWORDS: Autocatalytic cleavage;Direct protein sequencing;Hydrolase;Protease;Reference proteome P75548,"PROTEIN NAMES: HPr kinase/phosphorylase (HPrK/P) (HPr kinase/phosphatase) (HPr(Ser) kinase/phosphorylase) PROTEIN FAMILY: HPrK/P family ORGANISM: Mycoplasma pneumoniae (strain ATCC 29342 / M129 / Subtype 1) (Mycoplasmoides pneumoniae) SIMILARITY: Belongs to the HPrK/P family. FUNCTION: Is a metabolite-sensitive enzyme that catalyzes the ATP-as well as probably the pyrophosphate-dependent phosphorylation of Ser-47 in HPr, a phosphocarrier protein of the phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS). HprK/P also catalyzes the pyrophosphate-producing, inorganic phosphate-dependent dephosphorylation (phosphorolysis) of seryl-phosphorylated HPr (P-Ser-HPr). The regulatory role of HPrK/P in the physiology of M.pneumoniae is not known yet. KEYWORDS: 3D-structure;ATP-binding;Carbohydrate metabolism;Kinase;Magnesium;Metal-binding;Multifunctional enzyme;Nucleotide-binding;Reference proteome;Serine/threonine-protein kinase;Transferase MISCELLANEOUS: Both phosphorylation and phosphorolysis are carried out by the same active site and suggest a common mechanism for both reactions.; MISCELLANEOUS: Contrary to HPrK/P of other bacteria, that of M.pneumoniae has a very high affinity for ATP (Kd=5.3 microM), explaining kinase activity even at low ATP concentrations." P75682,"PROTEIN NAMES: Putative 2-dehydro-3-deoxy-D-gluconate aldolase YagE (KDG aldolase YagE) (Putative 2-dehydro-3-deoxy-D-pentonate aldolase YagE) PROTEIN FAMILY: DapA family ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the DapA family. FUNCTION: Catalyzes the formation of 2-keto-3-deoxy-gluconate (KDG) from pyruvate and glyceraldehyde. May also function as a 2-dehydro-3-deoxy-D-pentonate aldolase. Overexpression leads to increased growth (over 2 hours) in the presence of the antibiotics norfloxacin, ampicillin and streptomycin. KEYWORDS: 3D-structure;Cytoplasm;Lyase;Reference proteome;Schiff base SUBCELLULAR LOCATION: Cytoplasm MISCELLANEOUS: Part of prophage CP4-6." P75792,"PROTEIN NAMES: Sugar phosphatase YbiV PROTEIN FAMILY: HAD-like hydrolase superfamily, Cof family, SupH subfamily ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the HAD-like hydrolase superfamily. Cof family. SupH subfamily. FUNCTION: Catalyzes the hydrolysis of sugar phosphate to sugar and inorganic phosphate. Has a wide substrate specificity catalyzing the hydrolysis of ribose-5-phosphate, glucose-6-phosphate, fructose-1-phosphate, acetyl-phosphate, glycerol-1-phosphate, glycerol-2-phosphate, 2-deoxy-glucose-6-phosphate, mannose-6-phosphate and fructose-6-phosphate. Appears to have a low level of phosphotransferase activity using monophosphates as the phosphate donor. KEYWORDS: 3D-structure;Hydrolase;Magnesium;Metal-binding;Reference proteome " P75804,"PROTEIN NAMES: Aldose sugar dehydrogenase YliI (Asd) (Soluble aldose sugar dehydrogenase YliI) PROTEIN FAMILY: PQQ oxidoreductase GdhB family ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the PQQ oxidoreductase GdhB family. FUNCTION: Aldose sugar dehydrogenase with broad substrate specificity. The physiological substrate is unknown. Can oxidize glucose to gluconolactone. Can also utilize D-arabinose, L-arabinose and 2-deoxy-glucose. Has higher activity towards oligomeric sugars, such as maltose, maltotriose or cellobiose. It may function to input sugar-derived electrons into the respiratory network. KEYWORDS: 3D-structure;Calcium;Cell outer membrane;Membrane;Metal-binding;Oxidoreductase;PQQ;Reference proteome;Signal SUBCELLULAR LOCATION: Cell outer membrane " P75892,"PROTEIN NAMES: Putative pyrimidine permease RutG PROTEIN FAMILY: Nucleobase:cation symporter-2 (NCS2) family ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) family. FUNCTION: May function as a proton-driven pyrimidine uptake system. KEYWORDS: Cell inner membrane;Cell membrane;Membrane;Reference proteome;Symport;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein. MISCELLANEOUS: The Rut pathway degrades exogenous pyrimidines as the sole nitrogen source at room temperature but not at 37 degrees Celsius, a restriction that is apparently a consequence of an inadequate ability to remove toxic malonic semialdehyde at the higher temperature (RutE/YdfG function)." P75906,"PROTEIN NAMES: Poly-beta-1,6-N-acetyl-D-glucosamine N-deacetylase (PGA N-deacetylase) (Poly-beta-1,6-GlcNAc N-deacetylase) PROTEIN FAMILY: Polysaccharide deacetylase family ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the polysaccharide deacetylase family. FUNCTION: Catalyzes the N-deacetylation of poly-beta-1,6-N-acetyl-D-glucosamine (PGA), a biofilm adhesin polysaccharide. N-deacetylation promotes PGA export through the PgaA porin. KEYWORDS: 3D-structure;Cell outer membrane;Hydrolase;Lipoprotein;Membrane;Palmitate;Reference proteome;Signal SUBCELLULAR LOCATION: Cell outer membrane ; Lipid-anchor ; Periplasmic side " P75908,"PROTEIN NAMES: Probable diguanylate cyclase DgcT (DGC) ORGANISM: Escherichia coli (strain K12) FUNCTION: Probably catalyzes the synthesis of cyclic-di-GMP (c-di-GMP) via the condensation of 2 GTP molecules. Overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di-GMP is a second messenger which controls cell surface-associated traits in bacteria. PATHWAY: Purine metabolism; 3',5'-cyclic di-GMP biosynthesis. KEYWORDS: Cell inner membrane;Cell membrane;GTP-binding;Magnesium;Membrane;Metal-binding;Nucleotide-binding;Reference proteome;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell inner membrane ; Multi-pass membrane protein " P75960,"PROTEIN NAMES: NAD-dependent protein deacylase (Regulatory protein SIR2 homolog) PROTEIN FAMILY: Sirtuin family, Class III subfamily ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the sirtuin family. Class III subfamily. FUNCTION: NAD-dependent lysine deacetylase that specifically removes acetyl groups on target proteins. Also acts as a protein-lysine deacylase by mediating protein desuccinylation and de-2-hydroxyisobutyrylation. Modulates the activities of several proteins which are inactive in their acylated form. Activates the enzyme acetyl-CoA synthetase by deacetylating 'Lys-609' in the inactive, acetylated form of the enzyme. May also modulate the activity of other propionyl-adenosine monophosphate (AMP)-forming enzymes. KEYWORDS: 3D-structure;Alternative promoter usage;Cytoplasm;Metal-binding;NAD;Reference proteome;Transferase;Zinc SUBCELLULAR LOCATION: Cytoplasm " P76015,"PROTEIN NAMES: PEP-dependent dihydroxyacetone kinase, dihydroxyacetone-binding subunit DhaK ORGANISM: Escherichia coli (strain K12) FUNCTION: Dihydroxyacetone binding subunit of the dihydroxyacetone kinase, which is responsible for the phosphoenolpyruvate (PEP)-dependent phosphorylation of dihydroxyacetone via a phosphoryl group transfer from DhaL-ATP. Binds covalently dihydroxyacetone in hemiaminal linkage. DhaK acts also as corepressor of the transcription activator DhaR by binding to the sensor domain of DhaR. In the presence of dihydroxyacetone, DhaL-ADP displaces DhaK and stimulates DhaR activity. In the absence of dihydroxyacetone, DhaL-ADP is converted by the PTS to DhaL-ATP, which does not bind to DhaR. PATHWAY: Polyol metabolism; glycerol degradation. KEYWORDS: 3D-structure;Glycerol metabolism;Kinase;Reference proteome;Transferase MISCELLANEOUS: Unlike the carbohydrate-specific transporters of the PTS, the complex DhaKML has no transport activity." P76038,PROTEIN NAMES: Gamma-glutamyl-gamma-aminobutyrate hydrolase PuuD (Gamma-Glu-GABA hydrolase) PROTEIN FAMILY: Peptidase C26 family ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the peptidase C26 family. FUNCTION: Involved in the breakdown of putrescine via hydrolysis of the gamma-glutamyl linkage of gamma-glutamyl-gamma-aminobutyrate. PATHWAY: Amine and polyamine degradation; putrescine degradation; 4-aminobutanoate from putrescine: step 4/4. KEYWORDS: 3D-structure;Direct protein sequencing;Hydrolase;Reference proteome P76077,"PROTEIN NAMES: 1,2-phenylacetyl-CoA epoxidase, subunit A (1,2-phenylacetyl-CoA epoxidase, catalytic subunit alpha) (1,2-phenylacetyl-CoA monooxygenase, subunit A) ORGANISM: Escherichia coli (strain K12) FUNCTION: Component of 1,2-phenylacetyl-CoA epoxidase multicomponent enzyme system which catalyzes the reduction of phenylacetyl-CoA (PA-CoA) to form 1,2-epoxyphenylacetyl-CoA. The subunit A is the catalytic subunit involved in the incorporation of one atom of molecular oxygen into phenylacetyl-CoA. PATHWAY: Aromatic compound metabolism; phenylacetate degradation. KEYWORDS: 3D-structure;NADP;Oxidoreductase;Reference proteome " P76108,"PROTEIN NAMES: Bifunctional polyhydroxybutyrate synthase / ABC transporter periplasmic binding protein (Poly-3-hydroxybutyrate synthase) (PHB synthase) (cPHB synthase) PROTEIN FAMILY: Bacterial solute-binding protein PotD/PotF family ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the bacterial solute-binding protein PotD/PotF family. FUNCTION: Catalyzes the formation of short polymers of R-3-hydroxybutyrate (cPHB). Involved in natural transformation. Probably part of the ABC transporter complex YdcSTUV. During natural transformation, may bind dsDNA and convey it to the inner membrane channel formed by YdcV (Probable). KEYWORDS: Acyltransferase;Periplasm;PHB biosynthesis;Reference proteome;Signal;Transferase;Transport SUBCELLULAR LOCATION: Periplasm " P76113,"PROTEIN NAMES: NADPH-dependent curcumin reductase (NADPH-dependent curcumin/dihydrocurcumin reductase) PROTEIN FAMILY: NADP-dependent oxidoreductase L4BD family ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the NADP-dependent oxidoreductase L4BD family. FUNCTION: Catalyzes the metal-independent reduction of curcumin to dihydrocurcumin (DHC) as an intermediate product, followed by further reduction to tetrahydrocurcumin (THC) as an end product. It also acts on 3-octene-2-one, 3-hepten-2-one, resveratrol, and trans-2-octenal. KEYWORDS: NADP;Oxidoreductase;Reference proteome " P76577,"PROTEIN NAMES: Penicillin-binding protein 1C (PBP-1c) (PBP1c) [Includes: Penicillin-insensitive transglycosylase (Peptidoglycan TGase); Transpeptidase-like module] PROTEIN FAMILY: Glycosyltransferase 51 family; Transpeptidase family ORGANISM: Escherichia coli (strain K12) SIMILARITY: In the N-terminal section; belongs to the glycosyltransferase 51 family.; SIMILARITY: In the C-terminal section; belongs to the transpeptidase family. FUNCTION: Cell wall formation. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a transpeptidase C-terminal domain which may not be functional. PATHWAY: Cell wall biogenesis; peptidoglycan biosynthesis. KEYWORDS: Carboxypeptidase;Cell inner membrane;Cell membrane;Cell shape;Cell wall biogenesis/degradation;Glycosyltransferase;Hydrolase;Membrane;Multifunctional enzyme;Peptidoglycan synthesis;Protease;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell inner membrane ; Single-pass type II membrane protein MISCELLANEOUS: Due to the fact that PBP-1c can neither substitute for PBP-1a or PBP-1b, nor rescue a PBP-1a/PBP-1b double mutant, it is possible that PBP-1c has its own distinct function. Moreover it does not bind to most of the beta-lactams known to bind to other binding proteins, suggesting that the penicillin-binding domain must be different from those present in PBP-1a and PBP-1b. It may function as a transglycosylase only." P77172,PROTEIN NAMES: Cyclic di-GMP phosphodiesterase PdeF ORGANISM: Escherichia coli (strain K12) FUNCTION: Phosphodiesterase (PDE) that catalyzes the hydrolysis of cyclic-di-GMP (c-di-GMP) to 5'-pGpG. Truncated proteins consisting of the GGDEF/EAL domains (residues 319-747) or of the EAL domain alone (481-747) have c-di-GMP phosphodiesterase activity. They do not have diguanylate cyclase activity. Cyclic-di-GMP is a second messenger which controls cell surface-associated traits in bacteria. KEYWORDS: c-di-GMP;Cell inner membrane;Cell membrane;Hydrolase;Membrane;Nucleotide-binding;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell inner membrane ; Multi-pass membrane protein P77302,"PROTEIN NAMES: Diguanylate cyclase DgcM (DGC) ORGANISM: Escherichia coli (strain K12) FUNCTION: Part of a signaling cascade that regulates curli biosynthesis. The cascade is composed of two cyclic-di-GMP (c-di-GMP) control modules, in which c-di-GMP controlled by the DgcE/PdeH pair (module I) regulates the activity of the DgcM/PdeR pair (module II), which in turn regulates activity of the transcription factor MlrA and expression of the master biofilm regulator csgD. DgcM stimulates activity of MlrA by direct interaction, leading to the transcription of csgD. It also catalyzes the synthesis of c-di-GMP via the condensation of 2 GTP molecules, which contributes to the c-di-GMP pool generated by module I in a positive feedback loop. Production of c-di-GMP contributes to but is not essential for MlrA activation. PATHWAY: Purine metabolism; 3',5'-cyclic di-GMP biosynthesis. KEYWORDS: GTP-binding;Magnesium;Metal-binding;Nucleotide-binding;Reference proteome;Repeat;Transferase " P77366,"PROTEIN NAMES: Beta-phosphoglucomutase (Beta-PGM) PROTEIN FAMILY: HAD-like hydrolase superfamily, CbbY/CbbZ/Gph/YieH family ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family. FUNCTION: Catalyzes the conversion of beta D-glucose 1-phosphate (G1P) to D-glucose 6-phosphate (G6P), forming beta-D-glucose 1,6-(bis)phosphate (beta-G16P) as an intermediate (Probable). Phosphatase activity with the reaction intermediate beta-G16P has been measured. In vitro interconverts beta D-glucose 1-phosphate, beta-D-allose 1-phosphate, beta-D-galactose 1-phosphate and beta-D-mannose 1-phosphate to their corresponding sugar 6-phosphate product. The beta-D-glucose 1-phosphate substrate may be furnished by YcjT (AC P77154), the apparent upstream enzyme in the putative biochemical pathway encoded in this locus (yjcM to ycjW). It may play a key role in the regulation of the flow of carbohydrate intermediates in glycolysis and the formation of the sugar nucleotide UDP-glucose. KEYWORDS: 3D-structure;Carbohydrate metabolism;Cytoplasm;Isomerase;Magnesium;Metal-binding;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cytoplasm MISCELLANEOUS: The catalysis proceeds via a phosphoenzyme formed by reaction of an active-site nucleophile with the cofactor glucose 1,6-diphosphate (G1,6-diP). The phosphorylated mutase binds either G1P or G6P and transfers the phosphoryl group to the C(6)OH or C(1)OH, respectively (By similarity)." P77510,"PROTEIN NAMES: Sensor histidine kinase DpiB (Sensor histidine kinase CitA) ORGANISM: Escherichia coli (strain K12) FUNCTION: Member of the two-component regulatory system DpiA/DpiB, which is essential for expression of citrate-specific fermentation genes and genes involved in plasmid inheritance. Could be involved in response to both the presence of citrate and external redox conditions. Functions as a sensor kinase that phosphorylates DpiA in the presence of citrate. KEYWORDS: ATP-binding;Cell inner membrane;Cell membrane;Kinase;Membrane;Nucleotide-binding;Phosphoprotein;Reference proteome;Transferase;Transmembrane;Transmembrane helix;Two-component regulatory system SUBCELLULAR LOCATION: Cell inner membrane ; Multi-pass membrane protein " P77589,"PROTEIN NAMES: 3-(3-hydroxy-phenyl)propionate transporter (3HPP transporter) (3-(3-hydroxy-phenyl)propionate:H(+) symporter) (3HPP:H(+) symporter) PROTEIN FAMILY: Major facilitator superfamily, Aromatic acid:H(+) symporter (AAHS) family ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the major facilitator superfamily. Aromatic acid:H(+) symporter (AAHS) (TC 2.A.1.15) family. FUNCTION: Uptake of 3-(3-hydroxyphenyl)propionate (3HPP) across the cytoplasmic membrane. Transport is driven by the proton motive force. Does not transport benzoate, 3-hydroxybenzoate or gentisate. KEYWORDS: Cell inner membrane;Cell membrane;Membrane;Reference proteome;Symport;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell inner membrane ; Multi-pass membrane protein " P78314,"PROTEIN NAMES: SH3 domain-binding protein 2 (3BP-2) ORGANISM: Homo sapiens (Human) FUNCTION: Binds differentially to the SH3 domains of certain proteins of signal transduction pathways. Binds to phosphatidylinositols; linking the hemopoietic tyrosine kinase fes to the cytoplasmic membrane in a phosphorylation dependent mechanism. KEYWORDS: 3D-structure;Alternative splicing;Disease variant;Phosphoprotein;Reference proteome;SH2 domain;SH3-binding MISCELLANEOUS: [Isoform 2]: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay." P78333,"PROTEIN NAMES: Glypican-5 [Cleaved into: Secreted glypican-5] PROTEIN FAMILY: Glypican family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the glypican family. FUNCTION: Cell surface proteoglycan that bears heparan sulfate. KEYWORDS: Cell membrane;Glycoprotein;GPI-anchor;Heparan sulfate;Lipoprotein;Membrane;Proteoglycan;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Cell membrane ; Lipid-anchor, GPI-anchor ; Extracellular side.; SUBCELLULAR LOCATION: [Secreted glypican-5]: Secreted, extracellular space " P78371,"PROTEIN NAMES: T-complex protein 1 subunit beta (TCP-1-beta) (CCT-beta) (Chaperonin containing T-complex polypeptide 1 subunit 2) PROTEIN FAMILY: TCP-1 chaperonin family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the TCP-1 chaperonin family. FUNCTION: Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of proteins upon ATP hydrolysis. The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance. As part of the TRiC complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. The TRiC complex plays a role in the folding of actin and tubulin (Probable). KEYWORDS: 3D-structure;Acetylation;Alternative splicing;ATP-binding;Chaperone;Cytoplasm;Direct protein sequencing;Isopeptide bond;Nucleotide-binding;Phosphoprotein;Reference proteome;Ubl conjugation SUBCELLULAR LOCATION: Cytoplasm " P78382,"PROTEIN NAMES: CMP-sialic acid transporter (CMP-SA-Tr) (CMP-Sia-Tr) (CST) (Solute carrier family 35 member A1) PROTEIN FAMILY: Nucleotide-sugar transporter family, SLC35A subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the nucleotide-sugar transporter family. SLC35A subfamily. FUNCTION: Transports CMP-sialic acid from the cytosol into the Golgi apparatus, functioning as an antiporter that exchanges CMP-sialic acid for CMP. Binds both CMP-sialic acid and free CMP, but has higher affinity for free CMP (By similarity). Also able to exchange CMP-sialic acid for AMP and UMP. Also mediates the transport of CDP-ribitol (By similarity). KEYWORDS: Alternative splicing;Antiport;Congenital disorder of glycosylation;Disease variant;Golgi apparatus;Membrane;Reference proteome;Sugar transport;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Golgi apparatus membrane ; Multi-pass membrane protein Golgi apparatus " P78395,"PROTEIN NAMES: Melanoma antigen preferentially expressed in tumors (Opa-interacting protein 4) (OIP-4) (Preferentially expressed antigen of melanoma) PROTEIN FAMILY: PRAME family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the PRAME family. FUNCTION: Substrate-recognition component of a Cul2-RING (CRL2) E3 ubiquitin-protein ligase complex, which mediates ubiquitination of target proteins, leading to their degradation. The CRL2(PRAME) complex mediates ubiquitination and degradation of truncated MSRB1/SEPX1 selenoproteins produced by failed UGA/Sec decoding. In the nucleus, the CRL2(PRAME) complex is recruited to epigenetically and transcriptionally active promoter regions bound by nuclear transcription factor Y (NFY) and probably plays a role in chromstin regulation. Functions as a transcriptional repressor, inhibiting the signaling of retinoic acid through the retinoic acid receptors RARA, RARB and RARG: prevents retinoic acid-induced cell proliferation arrest, differentiation and apoptosis. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Apoptosis;Cell membrane;Chromosome;Cytoplasm;Differentiation;Golgi apparatus;Growth regulation;Leucine-rich repeat;Membrane;Nucleus;Phosphoprotein;Reference proteome;Repeat;Repressor;Transcription;Transcription regulation;Ubl conjugation pathway SUBCELLULAR LOCATION: Nucleus Chromosome Cytoplasm Golgi apparatus Cell membrane Note=Associates with chromatin; specifically enriched at transcriptionally active promoters that are also bound by nuclear transcription factor Y (composed of NFYA, NFYB and NFYC) and at enhancers. Recruited to the Golgi apparatus in response to interferon gamma (IFNG) treatment. MISCELLANEOUS: Tumor antigen recognized by cytolytic T lymphocytes." P78396,"PROTEIN NAMES: Cyclin-A1 PROTEIN FAMILY: Cyclin family, Cyclin AB subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the cyclin family. Cyclin AB subfamily. FUNCTION: May be involved in the control of the cell cycle at the G1/S (start) and G2/M (mitosis) transitions. May primarily function in the control of the germline meiotic cell cycle and additionally in the control of mitotic cell cycle in some somatic cells. KEYWORDS: Alternative splicing;Cell cycle;Cell division;Cyclin;Mitosis;Nucleus;Reference proteome;Ubl conjugation SUBCELLULAR LOCATION: Nucleus " P78413,PROTEIN NAMES: Iroquois-class homeodomain protein IRX-4 (Homeodomain protein IRXA3) (Iroquois homeobox protein 4) PROTEIN FAMILY: TALE/IRO homeobox family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the TALE/IRO homeobox family. FUNCTION: Likely to be an important mediator of ventricular differentiation during cardiac development. KEYWORDS: Alternative splicing;DNA-binding;Homeobox;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus P78424,"PROTEIN NAMES: POU domain, class 6, transcription factor 2 (Retina-derived POU domain factor 1) (RPF-1) PROTEIN FAMILY: POU transcription factor family, Class-6 subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the POU transcription factor family. Class-6 subfamily. FUNCTION: Probable transcription factor likely to be involved in early steps in the differentiation of amacrine and ganglion cells. Recognizes and binds to the DNA sequence 5'-ATGCAAAT-3'. Isoform 1 does not bind DNA. KEYWORDS: Alternative splicing;Disease variant;DNA-binding;Homeobox;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: [Isoform 1]: Major isoform." P78426,"PROTEIN NAMES: Homeobox protein Nkx-6.1 (Homeobox protein NK-6 homolog A) ORGANISM: Homo sapiens (Human) FUNCTION: Transcription factor which binds to specific A/T-rich DNA sequences in the promoter regions of a number of genes. Involved in the development of insulin-producing beta cells in the islets of Langerhans at the secondary transition (By similarity). Together with NKX2-2 and IRX3 acts to restrict the generation of motor neurons to the appropriate region of the neural tube. Belongs to the class II proteins of neuronal progenitor factors, which are induced by SHH signals (By similarity). KEYWORDS: Activator;Developmental protein;DNA-binding;Homeobox;Methylation;Nucleus;Reference proteome;Repressor;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " P78552,"PROTEIN NAMES: Interleukin-13 receptor subunit alpha-1 (IL-13 receptor subunit alpha-1) (IL-13R subunit alpha-1) (IL-13R-alpha-1) (IL-13RA1) (Cancer/testis antigen 19) (CT19) (CD antigen CD213a1) PROTEIN FAMILY: Type I cytokine receptor family, Type 5 subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the type I cytokine receptor family. Type 5 subfamily. FUNCTION: Binds with low affinity to interleukin-13 (IL13). Together with IL4RA can form a functional receptor for IL13. Also serves as an alternate accessory protein to the common cytokine receptor gamma chain for interleukin-4 (IL4) signaling, but cannot replace the function of IL2RG in allowing enhanced interleukin-2 (IL2) binding activity. KEYWORDS: 3D-structure;Alternative splicing;Disulfide bond;Glycoprotein;Membrane;Receptor;Reference proteome;Repeat;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. " P78581,PROTEIN NAMES: Tannase [Cleaved into: Tannase 33 kDa subunit; Tannase 30 kDa subunit] PROTEIN FAMILY: Tannase family ORGANISM: Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) SIMILARITY: Belongs to the tannase family. FUNCTION: Hydrolyzes ester bonds of tannic acid to produce gallic acid and glucose. KEYWORDS: Calcium;Cleavage on pair of basic residues;Direct protein sequencing;Disulfide bond;Glycoprotein;Hydrolase;Metal-binding;Pyrrolidone carboxylic acid;Reference proteome;Serine esterase;Signal P78759,"PROTEIN NAMES: Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta 2 isoform (PP2A, B subunit, B' delta 2 isoform) PROTEIN FAMILY: Phosphatase 2A regulatory subunit B family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the phosphatase 2A regulatory subunit B family. FUNCTION: The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. Has a role in cell shape control and septum formation. KEYWORDS: Cytoplasm;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Cell tip " P79005,"PROTEIN NAMES: Telomere length regulator taz1 ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) FUNCTION: Regulates telomere length and function. Required for the repression of telomere-adjacent gene expression and for normal meiosis or sporulation. It may be a negative regulator of the telomere-replicating enzyme, telomerase, or may protect against activation of telomerase-independent pathways of telomere elongation. It may be involved in the interactions between chromosomes and spindle proteins, disruption of these interactions would lead to defective meiosis. KEYWORDS: 3D-structure;Chromosome;Cytoplasm;DNA-binding;Nucleus;Phosphoprotein;Reference proteome;Repeat;Telomere SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Chromosome, telomere. " P79076,"PROTEIN NAMES: Pyranose 2-oxidase (P2Ox) (POD) (POx) (PROD) (Pyranose oxidase) (FAD-oxidoreductase) (Glucose 2-oxidase) (Pyranose:oxygen 2-oxidoreductase) PROTEIN FAMILY: GMC oxidoreductase family ORGANISM: Trametes versicolor (White-rot fungus) (Coriolus versicolor) SIMILARITY: Belongs to the GMC oxidoreductase family. FUNCTION: Catalyzes the oxidation of various aldopyranoses and disaccharides on carbon-2 to the corresponding 2-keto sugars concomitant with the reduction of O(2) to H(2)O(2). Plays an important role in lignin degradation of wood rot fungi by supplying the essential cosubstrate H(2)O(2) for the ligninolytic peroxidases, lignin peroxidase and manganese-dependent peroxidase. The preferred substrate is D-glucose which is converted to 2-dehydro-D-glucose. Acts also on D-xylose, together with D-glucose the major sugars derived from wood, on L-sorbose, D-galactose and 1,5-anhydroglucitol, a diagnostic marker of diabetes mellitus. KEYWORDS: Direct protein sequencing;FAD;Flavoprotein;Oxidoreductase;Periplasm;Signal SUBCELLULAR LOCATION: Periplasm Note=Hyphal periplasmic space. " P79106,"PROTEIN NAMES: Platelet-activating factor acetylhydrolase 2, cytoplasmic (PAF acetylhydrolase II) (PAF:lysophospholipid transacetylase) (PAF:sphingosine transacetylase) (Platelet-activating factor acetyltransferase PAFAH2) (Serine-dependent phospholipase A2) (SD-PLA2) PROTEIN FAMILY: Serine esterase family ORGANISM: Bos taurus (Bovine) SIMILARITY: Belongs to the serine esterase family. FUNCTION: Catalyzes the hydrolyze of the acetyl group at the sn-2 position of platelet-activating factor (PAF) and its analogs, leading to their inactivation. Hydrolyzes propionyl and butyroyl moieties approximately half as effectively as PAF. Also catalyzes transacetylation of the acetyl group from platelet-activating factor (PAF) to lysoplasmalogen and to sphingosine, producing plasmalogen analogs of PAF and N-acetylsphingosine (C2-ceramide) respectively. Has a marked selectivity for phospholipids with short acyl chains at the sn-2 position (By similarity). KEYWORDS: Cytoplasm;Direct protein sequencing;Endoplasmic reticulum;Hydrolase;Lipid degradation;Lipid metabolism;Lipoprotein;Membrane;Myristate;Reference proteome;Transferase SUBCELLULAR LOCATION: Cytoplasm Membrane ; Lipid-anchor Endoplasmic reticulum membrane ; Lipid-anchor Note=In resting cells, localizes to intracellular membranes and cytoplasm. Translocates from the cytoplasm to intracellular membranes upon oxidative stress. " P79760,"PROTEIN NAMES: Cytochrome P450 1A4 (CYPIA4) (Cytochrome P450 TCDDAHH) PROTEIN FAMILY: Cytochrome P450 family ORGANISM: Gallus gallus (Chicken) SIMILARITY: Belongs to the cytochrome P450 family. FUNCTION: Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. KEYWORDS: Endoplasmic reticulum;Heme;Iron;Membrane;Metal-binding;Microsome;Monooxygenase;Oxidoreductase;Reference proteome SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein. " P79762,"PROTEIN NAMES: Zona pellucida sperm-binding protein 3 (Sperm receptor) (Zona pellucida C protein) (Zona pellucida glycoprotein 3) (Zp-3) [Cleaved into: Processed zona pellucida sperm-binding protein 3] PROTEIN FAMILY: ZP domain family, ZPC subfamily ORGANISM: Gallus gallus (Chicken) SIMILARITY: Belongs to the ZP domain family. ZPC subfamily. FUNCTION: Component of the zona pellucida, which mediates species-specific sperm binding. Directly binds to sperm. Important for egg fertilization. KEYWORDS: 3D-structure;Cell membrane;Cleavage on pair of basic residues;Direct protein sequencing;Disulfide bond;Extracellular matrix;Fertilization;Glycoprotein;Membrane;Reference proteome;Secreted;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: [Processed zona pellucida sperm-binding protein 3]: Secreted, extracellular space, extracellular matrix Note=The glycoproteinaceous translucent extracellular matrix that surrounds the mammalian oocyte is called zona pellucida.; SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein " P81018,PROTEIN NAMES: Ladderlectin ORGANISM: Oncorhynchus mykiss (Rainbow trout) (Salmo gairdneri) FUNCTION: Lectin that binds sepharose in a calcium-dependent manner. KEYWORDS: Alternative splicing;Calcium;Direct protein sequencing;Disulfide bond;Lectin;Signal P81274,"PROTEIN NAMES: G-protein-signaling modulator 2 (Mosaic protein LGN) PROTEIN FAMILY: GPSM family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the GPSM family. FUNCTION: Plays an important role in mitotic spindle pole organization via its interaction with NUMA1. Required for cortical dynein-dynactin complex recruitment during metaphase. Plays a role in metaphase spindle orientation. Also plays an important role in asymmetric cell divisions. Has guanine nucleotide dissociation inhibitor (GDI) activity towards G(i) alpha proteins, such as GNAI1 and GNAI3, and thereby regulates their activity (By similarity). KEYWORDS: 3D-structure;Cell cycle;Cell division;Cell membrane;Cytoplasm;Cytoskeleton;Deafness;Membrane;Mitosis;Nucleotide-binding;Phosphoprotein;Reference proteome;Repeat;TPR repeat;Transport SUBCELLULAR LOCATION: Cytoplasm Cytoplasm, cell cortex Cytoplasm, cytoskeleton, spindle pole Lateral cell membrane Note=Localizes in the cytoplasm during interphase and at cell cortex during metaphase. Colocalizes with NUMA1 to mitotic spindle poles. Localized at the central and lateral cell cortex regions in a RanGTP-dependent manner. In horizontally retinal progenitor dividing cells, localized to the lateral cortical region. In vertically retinal progenitor dividing cells, localized at the polar cortical region (By similarity). MISCELLANEOUS: Dysfunction of LGN is associated with the phenotype of multiple micronuclei due to chromosomal mis-segregation and defect in cell division through mis-localization of mitotic spindle regulator protein NUMA1." P81299,"PROTEIN NAMES: Tyrosine-protein phosphatase cdc-14 (Cell division cycle-related protein 14) PROTEIN FAMILY: Protein-tyrosine phosphatase family, Non-receptor class CDC14 subfamily ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the protein-tyrosine phosphatase family. Non-receptor class CDC14 subfamily. FUNCTION: Protein phosphatase that negatively regulates the G1-to-S phase transition to inhibit the cell cycle and establish quiescence in cells of multiple lineages including vulval, hypodermal and intestinal. Promotes nuclear accumulation and activity of the cyclin-dependent kinase inhibitor cki-1 which leads to inhibition of G1 progression during vulval tissue development. Has been shown to not be required for cytokinesis. However, in the embryo, in a contrasting study, has been shown to act as a regulator of central spindle formation and cytokinesis, and may be required for localization of the spindle component zen-4, and its interacting partner air-2 at the spindle during late cell divisions.; FUNCTION: [Isoform c]: Main regulator of cell cycle arrest in vulval precursor cells. KEYWORDS: Alternative splicing;Cell cycle;Cell division;Cytoplasm;Cytoskeleton;Hydrolase;Microtubule;Nucleus;Protein phosphatase;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Cytoplasm, cytoskeleton, spindle Midbody Nucleus Note=Localizes to the cytoplasm throughout interphase. As cells enter prophase, accumulates at the centrosomes. During metaphase and anaphase, localizes to the spindle, in particular spindle asters and the spindle mid zone. During late telophase, localizes to cytoplasmic aggregates. In some studies, localizes to the midbody during late telophase. Localizes to the cytoplasm in G1-arrested cells. Localizes to the nucleus or nucleolus in postmitotic cells. Some studies report no localization to centrosomes or nuclei. Co-localizes with zen-4 at the spindle, and localization may be dependent on each other.; SUBCELLULAR LOCATION: [Isoform c]: Cytoplasm Nucleus Note=Localizes to the cytoplasm during interphase, but localizes to the nucleus during cell cycle arrest. " P81900,"PROTEIN NAMES: cAMP-dependent protein kinase type II regulatory subunit PROTEIN FAMILY: CAMP-dependent kinase regulatory chain family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the cAMP-dependent kinase regulatory chain family. FUNCTION: Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Mediates membrane association by binding to anchoring proteins, such as Akap200. Might play an essential role in the regulation of neuronal activity in the brain. KEYWORDS: Alternative splicing;Biological rhythms;cAMP;cAMP-binding;Cell membrane;Cytoplasm;Direct protein sequencing;Membrane;Nucleotide-binding;Phosphoprotein;Reference proteome;Repeat SUBCELLULAR LOCATION: Cytoplasm Cell membrane Note=Localization to the membrane depends on Akap200. " P81917,"PROTEIN NAMES: Odorant receptor 43a PROTEIN FAMILY: Insect chemoreceptor superfamily, Heteromeric odorant receptor channel family, Or30a subfamily ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the insect chemoreceptor superfamily. Heteromeric odorant receptor channel (TC 1.A.69) family. Or30a subfamily. FUNCTION: Odorant receptor which mediates acceptance or avoidance behavior, depending on its substrates. The odorant receptor repertoire encodes a large collection of odor stimuli that vary widely in identity, intensity, and duration. Complexes with Orco to form odorant-sensing units, providing sensitive and prolonged odorant signaling and calcium permeability. They are necessary and sufficient to promote functional reconstitution of odor-evoked signaling in sensory neurons that normally respond only to carbon dioxide. Involved in the behavioral responses to acetophenone, benzaldehyde, benzyl alcohol, cyclohexanone, and cyclohexanol. KEYWORDS: Cell membrane;Membrane;Olfaction;Receptor;Reference proteome;Sensory transduction;Transducer;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein MISCELLANEOUS: The atypical heteromeric and topological design of the odorant receptors appears to be an insect-specific solution for odor recognition, making the OR/Orco complex an attractive target for the development of highly selective insect repellents to disrupt olfactory-mediated host-seeking behaviors of insect disease vectors. Odor-evoked OR currents are independent of known G-protein-coupled second messenger pathways." P82281,"PROTEIN NAMES: Thylakoid lumenal 29 kDa protein, chloroplastic (TL29) (AtAPx07) (P29) (Probable L-ascorbate peroxidase 4) PROTEIN FAMILY: Peroxidase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the peroxidase family. KEYWORDS: 3D-structure;Chloroplast;Direct protein sequencing;Oxidoreductase;Phosphoprotein;Plastid;Reference proteome;Thylakoid;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid lumen " P82350,"PROTEIN NAMES: Alpha-sarcoglycan (Alpha-SG) (50 kDa dystrophin-associated glycoprotein) (50DAG) (Adhalin) PROTEIN FAMILY: Sarcoglycan alpha/epsilon family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the sarcoglycan alpha/epsilon family. FUNCTION: Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix. KEYWORDS: Cell membrane;Cytoplasm;Cytoskeleton;Glycoprotein;Membrane;Phosphoprotein;Reference proteome;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane, sarcolemma ; Single-pass type I membrane protein Cytoplasm, cytoskeleton " P82450,"PROTEIN NAMES: Sialate O-acetylesterase (Sialic acid-specific 9-O-acetylesterase) (Yolk sac protein 2) [Cleaved into: Sialate O-acetylesterase small subunit; Sialate O-acetylesterase large subunit] ORGANISM: Rattus norvegicus (Rat) FUNCTION: Catalyzes the removal of O-acetyl ester groups from position 9 of the parent sialic acid, N-acetylneuraminic acid. KEYWORDS: Direct protein sequencing;Disulfide bond;Glycoprotein;Hydrolase;Lysosome;Reference proteome;Serine esterase;Signal SUBCELLULAR LOCATION: Lysosome. " P82476,"PROTEIN NAMES: Chitin deacetylase 3 PROTEIN FAMILY: Polysaccharide deacetylase family ORGANISM: Cryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487) (Filobasidiella neoformans var. grubii) SIMILARITY: Belongs to the polysaccharide deacetylase family. FUNCTION: Hydrolyzes the N-acetamido groups of N-acetyl-D-glucosamine residues in chitin to form chitosan and acetate. Chitosan is required to anchor melanin to the cell wall, for maintenance of cell wall integrity, and for proper cytokinesis. Chitosan offers an advantage during infection as it is less readily detected than chitin by host immunosurveillance mechanisms. KEYWORDS: Carbohydrate metabolism;Cell membrane;Cell wall biogenesis/degradation;Chitin degradation;Chitin-binding;Cleavage on pair of basic residues;Cobalt;Direct protein sequencing;Glycoprotein;GPI-anchor;Hydrolase;Lipoprotein;Membrane;Metal-binding;Polysaccharide degradation;Signal SUBCELLULAR LOCATION: Cell membrane ; Lipid-anchor, GPI-anchor " P82600,"PROTEIN NAMES: Chorion peroxidase PROTEIN FAMILY: Peroxidase family, XPO subfamily ORGANISM: Aedes aegypti (Yellowfever mosquito) (Culex aegypti) SIMILARITY: Belongs to the peroxidase family. XPO subfamily. FUNCTION: Involved in the formation of a rigid and insoluble egg chorion by catalyzing chorion protein cross-linking through dityrosine formation and phenol oxidase-catalyzed chorion melanization. KEYWORDS: Acetylation;Cleavage on pair of basic residues;Direct protein sequencing;Disulfide bond;Glycoprotein;Heme;Hydrogen peroxide;Hydroxylation;Iron;Metal-binding;Oxidoreductase;Peroxidase;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted. Note=In the chorion layer of the mature eggs. " P82650,"PROTEIN NAMES: Small ribosomal subunit protein mS22 (28S ribosomal protein S22, mitochondrial) (MRP-S22) (S22mt) PROTEIN FAMILY: Mitochondrion-specific ribosomal protein mS22 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the mitochondrion-specific ribosomal protein mS22 family. KEYWORDS: 3D-structure;Acetylation;Alternative splicing;Disease variant;Mitochondrion;Phosphoprotein;Primary mitochondrial disease;Reference proteome;Ribonucleoprotein;Ribosomal protein SUBCELLULAR LOCATION: Mitochondrion " P82873,"PROTEIN NAMES: Mitochondrial import receptor subunit TOM20-2 (Translocase of outer membrane 20 kDa subunit 2) PROTEIN FAMILY: Tom20 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the Tom20 family. FUNCTION: Central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the translocation pore. KEYWORDS: Acetylation;Direct protein sequencing;Membrane;Mitochondrion;Mitochondrion outer membrane;Protein transport;Reference proteome;Repeat;TPR repeat;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Mitochondrion outer membrane ; Single-pass membrane protein MISCELLANEOUS: There are four genes (TOM20-1, TOM20-2, TOM20-3 and TOM20-4) which encode mitochondrial import receptor subunits TOM20.; MISCELLANEOUS: In mammals and fungi, the transmembrane domain is located at the N-terminus while it is located at the C-terminus in plants. The overall orientation of the protein in the membrane is therefore inverted." P83094,PROTEIN NAMES: Stromal interaction molecule homolog ORGANISM: Drosophila melanogaster (Fruit fly) FUNCTION: Plays a role in mediating Ca(2+) influx following depletion of intracellular Ca(2+) stores. KEYWORDS: Calcium;Calcium transport;Cell membrane;Coiled coil;Glycoprotein;Ion transport;Membrane;Metal-binding;Phosphoprotein;Reference proteome;Signal;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein P83100,"PROTEIN NAMES: Putative mitogen-activated protein kinase 14C (MAP kinase 14C) (MAPK 14C) (MAP kinase p38c) PROTEIN FAMILY: Protein kinase superfamily, CMGC Ser/Thr protein kinase family, MAP kinase subfamily ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. FUNCTION: Kinase involved in a signal transduction pathway. KEYWORDS: ATP-binding;Kinase;Nucleotide-binding;Phosphoprotein;Reference proteome;Serine/threonine-protein kinase;Transferase " P83103,"PROTEIN NAMES: Serine/threonine-protein kinase haspin homolog PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family, Haspin subfamily ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Haspin subfamily. FUNCTION: Serine/threonine-protein kinase that phosphorylates histone H3 at 'Thr-4' (H3T3ph) during mitosis and interphase. Function is essential for chromosome organization during mitosis and genome organization in interphase cells, thus playing a functional role in gene regulation. During mitosis, may act through H3T3ph to both position and modulate activation of AURKB and other components of the chromosomal passenger complex (CPC) at centromeres to ensure proper chromatid cohesion, metaphase alignment and normal progression through the cell cycle (By similarity). During interphase, associates with the cohesion complex and mediates pds5 binding to chromatin to ensure correct sister chromatid cohesion, chromatin organization, and also functions with Pds5-cohesin to modify Polycomb-dependent homeotic transformations. Function during interphase is required for insulator activity, nuclear compaction, heterochromatin-induced position-effect variegation and PcG-mediated pairing-sensitive silencing. KEYWORDS: ATP-binding;Chromosome;Cytoplasm;Cytoskeleton;Kinase;Nucleotide-binding;Nucleus;Phosphoprotein;Reference proteome;Serine/threonine-protein kinase;Transferase SUBCELLULAR LOCATION: Nucleus lamina Chromosome Cytoplasm, cytoskeleton, spindle " P83513,"PROTEIN NAMES: Bifunctional xylanase/deacetylase [Includes: Endo-1,4-beta-xylanase 11A (Xylanase XynT) (Xylanase xyn11A); Acetylated xylan deacetylase ] PROTEIN FAMILY: Glycosyl hydrolase 11 (cellulase G) family ORGANISM: Pseudobutyrivibrio xylanivorans SIMILARITY: Belongs to the glycosyl hydrolase 11 (cellulase G) family. FUNCTION: Endo-acting xylanase which specifically cleaves internal linkages on the xylan backbone, releasing xylooligosaccharides. Is also probably able, via its C-terminal domain, to remove acetyl groups from acetylated xylan, and thus it is probably capable of hydrolyzing acetylated xylan. PATHWAY: Glycan degradation; xylan degradation. KEYWORDS: Carbohydrate metabolism;Direct protein sequencing;Glycosidase;Hydrolase;Multifunctional enzyme;Polysaccharide degradation;Secreted;Signal;Xylan degradation SUBCELLULAR LOCATION: Secreted " P83886,"PROTEIN NAMES: Mannose-specific lectin (ASAL) (ASARI) (Allimin) (Leaf agglutinin) (Root agglutinin) ORGANISM: Allium sativum (Garlic) FUNCTION: Mannose-specific lectin. Shows agglutinating activity towards rabbit erythrocytes. However, it does not show agglutinating activity towards human erythrocytes. Has insecticidal activity against the cotton leafworm S.littoralis and the peach potato aphid M.persicae. Also displays antiviral activity and therefore may contribute to defense against infections. KEYWORDS: Alternative splicing;Antiviral protein;Direct protein sequencing;Disulfide bond;Lectin;Mannose-binding;Plant defense;Secreted;Signal SUBCELLULAR LOCATION: Secreted MISCELLANEOUS: Has an inhibitory effect on SARS-CoV-2 in Vero E6 cells (IC(50)=4 ug/ml). Viral replication is inhibited by 80% at a concentration of 20 ug/ml, and 50% inhibition is observed at concentrations of 5 ug/ml and 10 ug/ml." P83949,PROTEIN NAMES: Homeotic protein ultrabithorax PROTEIN FAMILY: Antp homeobox family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the Antp homeobox family. FUNCTION: Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Binds the consensus region 5'-TTAAT[GT][GA]-3'. This homeotic protein controls development of the cells in the posterior thoracic and first abdominal segments. It activates the synthesis of the decapentaplegic (DPP) growth factor. KEYWORDS: 3D-structure;Activator;Alternative splicing;Developmental protein;DNA-binding;Homeobox;Nucleus;Phosphoprotein;Reference proteome;Repressor;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus. P84193,"PROTEIN NAMES: Aldos-2-ulose dehydratase (AUDH) (Aldos-2-ulose dehydratase/isomerase) (D-arabino-hex-2-ulose dehydratase) (Pyranosone dehydratase) ORGANISM: Phanerodontia chrysosporium (White-rot fungus) (Sporotrichum pruinosum) FUNCTION: A bifunctional enzyme which catalyzes the dehydration of anhydrofructose into ascopyrone M, and the isomerization of ascopyrone M into microthecin. To a lesser extent, can also act on 2-dehydro-D-glucopyranose (D-glucosone), leading to the antibiotic cortalcerone. PATHWAY: Carbohydrate metabolism; 1,5-anhydro-D-fructose degradation. KEYWORDS: 3D-structure;Direct protein sequencing;Isomerase;Lyase;Magnesium;Metal-binding;Multifunctional enzyme;Zinc " P84875,"PROTEIN NAMES: Carboxypeptidase inhibitor SmCI ORGANISM: Sabellastarte magnifica (Feather duster) FUNCTION: Potent inhibitor of pancreatic carboxypeptidase A with a Ki of 27 nM, and of the serine proteases trypsin, chymotrypsin and pancreatic elastase, with Ki values of 6.9 nM, 1.83 nM and 4 nM, respectively (Ref.1). Also inhibits carboxypeptidase A4 CPA4 and CPA1. Does not inhibit cysteine and aspartic proteases (Ref.1). KEYWORDS: 3D-structure;Direct protein sequencing;Disulfide bond;Glycoprotein;Metalloenzyme inhibitor;Metalloprotease inhibitor;Protease inhibitor;Repeat;Serine protease inhibitor " P84888,"PROTEIN NAMES: Aralkylamine dehydrogenase heavy chain (Aromatic amine dehydrogenase) (AADH) PROTEIN FAMILY: Aromatic amine dehydrogenase heavy chain family ORGANISM: Alcaligenes faecalis SIMILARITY: Belongs to the aromatic amine dehydrogenase heavy chain family. FUNCTION: Oxidizes primary aromatic amines and, more slowly, some long-chain aliphatic amines, but not methylamine or ethylamine. Uses azurin as an electron acceptor to transfer electrons from the reduced tryptophylquinone cofactor. KEYWORDS: 3D-structure;Disulfide bond;Electron transport;Oxidoreductase;Periplasm;Pyrrolidone carboxylic acid;Signal;Transport SUBCELLULAR LOCATION: Periplasm " P85051,"PROTEIN NAMES: Dual specificity tyrosine-phosphorylation-regulated kinase 1A (Dual specificity YAK1-related kinase) (Protein kinase minibrain homolog) (MNBH) (RP86) PROTEIN FAMILY: Protein kinase superfamily, CMGC Ser/Thr protein kinase family, MNB/DYRK subfamily ORGANISM: Oryctolagus cuniculus (Rabbit) SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily. FUNCTION: Dual-specificity kinase which possesses both serine/threonine and tyrosine kinase activities. Exhibits a substrate preference for proline at position P+1 and arginine at position P-3. Plays an important role in double-strand breaks (DSBs) repair following DNA damage. Mechanistically, phosphorylates RNF169 and increases its ability to block accumulation of TP53BP1 at the DSB sites thereby promoting homologous recombination repair (HRR). Also acts as a positive regulator of transcription by acting as a CTD kinase that mediates phosphorylation of the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II) POLR2A. May play a role in a signaling pathway regulating nuclear functions of cell proliferation (By similarity). Modulates alternative splicing by phosphorylating the splice factor SRSF6 (By similarity). Has pro-survival function and negatively regulates the apoptotic process. Promotes cell survival upon genotoxic stress through phosphorylation of SIRT1. This in turn inhibits p53/TP53 activity and apoptosis. Phosphorylates SEPTIN4, SEPTIN5 and SF3B1 at 'Thr-434' (By similarity). KEYWORDS: ATP-binding;Direct protein sequencing;Kinase;Nucleotide-binding;Nucleus;Phosphoprotein;Reference proteome;Serine/threonine-protein kinase;Transcription;Transcription regulation;Transferase;Tyrosine-protein kinase SUBCELLULAR LOCATION: Nucleus " P85298,PROTEIN NAMES: Rho GTPase-activating protein 8 (Rho-type GTPase-activating protein 8) ORGANISM: Homo sapiens (Human) FUNCTION: GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state. KEYWORDS: Alternative splicing;GTPase activation;Reference proteome P86100,"PROTEIN NAMES: Hyaluronidase-1 (BmHYA1) (HYA1) (Hyaluronoglucosaminidase-1) (Venom spreading factor) PROTEIN FAMILY: Glycosyl hydrolase 56 family ORGANISM: Mesobuthus martensii (Manchurian scorpion) (Buthus martensii) SIMILARITY: Belongs to the glycosyl hydrolase 56 family. FUNCTION: Hydrolyzes high molecular weight hyaluronic acid to produce small oligosaccharides. In venom, it may participate in the degradation of the extracellular matrix thus allowing a rapid spread of other venom components during the envenomation process. KEYWORDS: Direct protein sequencing;Disulfide bond;EGF-like domain;Glycoprotein;Glycosidase;Hydrolase;Secreted;Signal SUBCELLULAR LOCATION: Secreted " P86926,"PROTEIN NAMES: RNA-editing ligase 1, mitochondrial (RNA ligase 1) PROTEIN FAMILY: RNA ligase 2 family ORGANISM: Trypanosoma brucei brucei SIMILARITY: Belongs to the RNA ligase 2 family. FUNCTION: Essential for RNA editing. RNA editing in kinetoplastid mitochondria inserts and deletes uridylates at multiple sites in pre-mRNAs as directed by guide RNAs. KEYWORDS: ATP-binding;Direct protein sequencing;Ligase;Mitochondrion;mRNA processing;Nucleotide-binding;RNA-binding;Transit peptide SUBCELLULAR LOCATION: Mitochondrion " P87050,"PROTEIN NAMES: Mitosis inducer protein kinase cdr2 PROTEIN FAMILY: Protein kinase superfamily, CAMK Ser/Thr protein kinase family, NIM1 subfamily ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. NIM1 subfamily. FUNCTION: Acts as a mitotic inducer. In G2 it negatively regulates wee1, a mitotic inhibitor. Also has a role in cytokinesis where it required for proper septum formation. KEYWORDS: ATP-binding;Cell cycle;Cell division;Kinase;Mitosis;Nucleotide-binding;Phosphoprotein;Reference proteome;Serine/threonine-protein kinase;Transferase " P87074,"PROTEIN NAMES: DNA repair protein crb2 (Checkpoint mediator protein crb2) (Cut5-repeat binding protein 2) (RAD9 protein homolog) ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) FUNCTION: Essential for cell cycle arrest at the G1 and G2 stages following DNA damage by X-, and UV-irradiation, or inactivation of DNA ligase. Plays a role in the response to DNA damage. Interaction with rad4 via its phosphorylation sites in the N-terminus couples the DNA checkpoint apparatus to chromatin via interaction of its C-terminal BRCT domains with epigenetic modifications on histones H4 and H2A, respectively, in the G1/S phase of the cell cycle, and facilitates recruitment of the checkpoint kinase chk1. KEYWORDS: 3D-structure;Cell cycle;DNA damage;DNA replication inhibitor;Nucleus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Nucleus Note=Recruited to sites of DNA damage, such as double stand breaks. " P87175,PROTEIN NAMES: DNA-(apurinic or apyrimidinic site) endonuclease 2 (AP endonuclease 2) (Apurinic-apyrimidinic endonuclease 2) PROTEIN FAMILY: DNA repair enzymes AP/ExoA family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the DNA repair enzymes AP/ExoA family. FUNCTION: DNA repair enzyme that cleaves apurinic/apyrimidinic (AP) sites and removes 3'-blocking groups present at single strand breaks of damaged DNA. Provides the majority of the AP-endonuclease (APE) activity. Repairs phleomycin D1-induced DNA damage. Plays a role in oxidative damage repair. KEYWORDS: DNA damage;DNA repair;Hydrolase;Magnesium;Metal-binding;Nucleus;Reference proteome;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus P87324,PROTEIN NAMES: DNA polymerase subunit delta-2 PROTEIN FAMILY: DNA polymerase delta/II small subunit family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the DNA polymerase delta/II small subunit family. FUNCTION: Required for replication of the leading DNA strand and for completion of lagging strand synthesis. It is essential for cell cycle progression. KEYWORDS: Cell cycle;Cell division;DNA replication;DNA-directed DNA polymerase;Mitosis;Nucleotidyltransferase;Nucleus;Reference proteome;Transferase SUBCELLULAR LOCATION: Nucleus. P90794,PROTEIN NAMES: DDB1- and CUL4-associated factor 11 homolog (WD repeat-containing protein 23) PROTEIN FAMILY: WD repeat LEC14B family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the WD repeat LEC14B family. FUNCTION: Involved in regulation of lifespan. Required for dopaminergic CEP neuron degeneration in response to Mn(2+). Inhibits the skn-1-mediated up-regulation of tatn-1. KEYWORDS: Alternative splicing;Reference proteome;Repeat;WD repeat P90820,"PROTEIN NAMES: Heme peroxidase 2 [Cleaved into: Heme peroxidase 2 light chain; Heme peroxidase 2 heavy chain] PROTEIN FAMILY: Peroxidase family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the peroxidase family. FUNCTION: Peroxidase which is involved in maintaining the cuticle integrity in the hypodermis and pharynx. It thus plays a role in conferring resistance against Gram-positive bacteria such as E.faecalis, S.aureus and C.diphtheriae, and yeast such as C.albicans. KEYWORDS: Calcium;Disulfide bond;Glycoprotein;Heme;Iron;Metal-binding;Oxidoreductase;Peroxidase;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted " P90897,"PROTEIN NAMES: DEAD-box ATP-dependent RNA helicase rde-12 (RNA interference defective protein 12) PROTEIN FAMILY: DEAD box helicase family, DDX3/DED1 subfamily ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the DEAD box helicase family. DDX3/DED1 subfamily. FUNCTION: Probable ATP-dependent RNA helicase involved in RNAi-mediated gene silencing. Specifically required in the endogenous siRNA pathway for biogenesis of secondary endogenous small interfering RNA (siRNA) intermediates called 22G-RNAs. May associate with and recruit rde-10 to primary siRNA-targeted mRNA for secondary siRNA synthesis. May be recruited to target mRNAs by rde-1 and/or ergo-1. KEYWORDS: Alternative splicing;ATP-binding;Cytoplasm;Helicase;Hydrolase;Nucleotide-binding;Reference proteome;Repressor;RNA-mediated gene silencing;Translation regulation SUBCELLULAR LOCATION: Cytoplasm, perinuclear region Cytoplasmic granule Cytoplasm Cytoplasm, P-body Note=Colocalizes with pgl-1 in perinuclear P granules. Colocalizes with rsd-6 in a subset of germline and embryonic foci. " P91124,PROTEIN NAMES: Resistance to inhibitors of cholinesterase protein 19 (ICA1 homolog) ORGANISM: Caenorhabditis elegans FUNCTION: May be involved in neurotransmitter secretion. In association with the GTPase activator protein tbc-8 activates rab-2 during dense core vesicle maturation in cholinergic motoneurons. KEYWORDS: Cytoplasm;Cytoplasmic vesicle;Membrane;Neurotransmitter transport;Reference proteome;Transport SUBCELLULAR LOCATION: Cytoplasm Cytoplasmic vesicle membrane ; Peripheral membrane protein Note=Co-localizes with tbc-8 at cytoplasmic vesicle membranes in neurons. P91277,"PROTEIN NAMES: Heterogeneous nuclear ribonucleoprotein K homolog (hnRNP K) ORGANISM: Caenorhabditis elegans FUNCTION: RNA-binding protein which functions together with alg-1, a component of the miRNA loading complex, to modulate the processing and activity of specific miRNAs such as miR-58 and let-7 to regulate gene expression at the post-transcriptional level during embryonic, hypodermal and neuronal development. Promotes the lsy-6-mediated repression of cog-1 in uterine cells. In embryos, may play a role in the DNA damage response. KEYWORDS: Alternative splicing;Cytoplasm;Nucleus;Reference proteome;Repeat;RNA-binding;RNA-mediated gene silencing SUBCELLULAR LOCATION: Nucleus Cytoplasm Note=Localizes to the cytoplasm and nucleus in the germline, oocytes and early embryos. Does not localize to the cytoplasm of somatic cells. " P91679,"PROTEIN NAMES: Peptide transporter family 1 (Oligopeptide transporter 1) (Protein YIN) PROTEIN FAMILY: Major facilitator superfamily, Proton-dependent oligopeptide transporter (POT/PTR) family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the major facilitator superfamily. Proton-dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family. FUNCTION: Important role in absorption of dietary peptides. High-affinity transporter of alanylalanine. Dipeptide transport activity is proton dependent. KEYWORDS: Alternative splicing;Membrane;Peptide transport;Protein transport;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Membrane ; Multi-pass membrane protein " P91829,"PROTEIN NAMES: Nuclear hormone receptor family member nhr-69 PROTEIN FAMILY: Nuclear hormone receptor family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the nuclear hormone receptor family. FUNCTION: Orphan nuclear receptor which, in cooperation with R-SMAD daf-8, modulates the Insulin/IGF-1-like signaling (IIS) pathway, perhaps by regulating expression of the potassium channel exp-2, which in turn modulates the secretion of insulin-like peptide daf-28. KEYWORDS: DNA-binding;Metal-binding;Nucleus;Receptor;Reference proteome;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus " P91859,"PROTEIN NAMES: Microtubule-associated protein homolog maph-1.1 PROTEIN FAMILY: MAP1A/MAP1B/MAP1S family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the MAP1A/MAP1B/MAP1S family. KEYWORDS: Alternative splicing;Cell projection;Cytoplasm;Cytoskeleton;Reference proteome SUBCELLULAR LOCATION: Cell projection, dendrite Perikaryon Cell projection, axon Cytoplasm, cytoskeleton Note=Localizes to microtubules in the neuronal cell body, axon and dendrites. " P91929,"PROTEIN NAMES: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial (Complex I-42kD) (CI-42kD) (NADH-ubiquinone oxidoreductase 42 kDa subunit) PROTEIN FAMILY: Complex I NDUFA10 subunit family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the complex I NDUFA10 subunit family. FUNCTION: Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. KEYWORDS: 3D-structure;Cytoplasm;Electron transport;FAD;Flavoprotein;Mitochondrion;Reference proteome;Respiratory chain;Transit peptide;Transport SUBCELLULAR LOCATION: Mitochondrion matrix Cytoplasm " P91953,PROTEIN NAMES: 50 kDa hatching enzyme (HE) (HEZ) (Envelysin) (Sea-urchin-hatching proteinase) [Cleaved into: 38 kDa hatching enzyme; 32 kDa hatching enzyme non-specific; 15 kDa peptide] PROTEIN FAMILY: Peptidase M10A family ORGANISM: Hemicentrotus pulcherrimus (Sea urchin) (Strongylocentrotus pulcherrimus) SIMILARITY: Belongs to the peptidase M10A family. FUNCTION: Allows the sea urchin to digest the protective envelope derived from the egg extracellular matrix; thus allowing the sea urchin to swim freely. KEYWORDS: Autocatalytic cleavage;Calcium;Direct protein sequencing;Disulfide bond;Glycoprotein;Hydrolase;Metal-binding;Metalloprotease;Protease;Repeat;Signal;Zinc;Zymogen P92948,PROTEIN NAMES: Cell division cycle 5-like protein (Cdc5-like protein) (Atypical R2R3-MYB transcription factor CDC5) (MOS4-associated complex protein 1) (MAC protein 1) (Protein MYB DOMAIN CELL DIVISION CYCLE 5) (AtMYBCD5) PROTEIN FAMILY: CEF1 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the CEF1 family. FUNCTION: Component of the MAC complex that probably regulates defense responses through transcriptional control and thereby is essential for plant innate immunity. Possesses a sequence specific DNA sequence 'CTCAGCG' binding activity. Involved in mRNA splicing and cell cycle control. May also play a role in the response to DNA damage. KEYWORDS: Activator;Cell cycle;Coiled coil;DNA damage;DNA repair;DNA-binding;Immunity;Innate immunity;mRNA processing;mRNA splicing;Nucleus;Phosphoprotein;Plant defense;Reference proteome;Repeat;Spliceosome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus P93024,PROTEIN NAMES: Auxin response factor 5 (Auxin-responsive protein IAA24) (Transcription factor MONOPTEROS) PROTEIN FAMILY: ARF family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the ARF family. FUNCTION: Auxin response factors (ARFs) are transcriptional factors that bind specifically to the DNA sequence 5'-TGTCTC-3' found in the auxin-responsive promoter elements (AuxREs). Seems to act as transcriptional activator. Formation of heterodimers with Aux/IAA proteins may alter their ability to modulate early auxin response genes expression. Mediates embryo axis formation and vascular tissues differentiation. Functionally redundant with ARF7. May be necessary to counteract AMP1 activity. KEYWORDS: 3D-structure;Activator;Auxin signaling pathway;Developmental protein;DNA-binding;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus. MISCELLANEOUS: Absence of the protein probably causes early embryonic lethality. Premature stop codons are associated with vascular defects. P93193,PROTEIN NAMES: Ipomoelin (Jacalin related lectin) (JRL) PROTEIN FAMILY: Jacalin lectin family ORGANISM: Ipomoea batatas (Sweet potato) (Convolvulus batatas) SIMILARITY: Belongs to the jacalin lectin family. FUNCTION: Lectin involved in defense reactions of the leaves in response to wounding by herbivorous insects and pathogens (Probable). Retards the growth and development of silkworm thus reducing their survival rates. Has hemagglutinating activity against human erythrocytes (Ref.4). KEYWORDS: 3D-structure;Direct protein sequencing;Lectin P93243,"PROTEIN NAMES: Aliphatic (R)-hydroxynitrile lyase (LuHNL) PROTEIN FAMILY: Zinc-containing alcohol dehydrogenase family ORGANISM: Linum usitatissimum (Flax) (Linum humile) SIMILARITY: Belongs to the zinc-containing alcohol dehydrogenase family. FUNCTION: Involved in the catabolism of cyanogenic glycosides. Naturally occurring substrates are the aliphatic acetone cyanohydrin and butan-2-one cyanohydrin, which are the aglycones of the cyanogenic glycosides linamarin, lotaustralin, linustatin and neolinustatin. Can use various aliphatic ketones and aldehydes as substrates, but not aromatic ketones. KEYWORDS: 3D-structure;Direct protein sequencing;Lyase;Metal-binding;Zinc MISCELLANEOUS: In contrast to the enzyme from rosaceae, this enzyme is not glycosylated and does not contain FAD. Not inhibited by reagents interfering with Zn(2+) coordination." P93324,"PROTEIN NAMES: Isoliquiritigenin 2'-O-methyltransferase (MsCHMT) (Chalcone O-methyltransferase) (ChOMT) (Licodione 2'-O-methyltransferase) (MsLMT) PROTEIN FAMILY: Class I-like SAM-binding methyltransferase superfamily, Cation-independent O-methyltransferase family, COMT subfamily ORGANISM: Medicago sativa (Alfalfa) SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O-methyltransferase family. COMT subfamily. FUNCTION: Methylates the 2'-hydroxyl of isoliquiritigenin and licodione. Does not methylate narigenin chalcone, caffeic acid or daidzein. Involved in the root nodulation initiation by promoting the biosynthesis of nod-inducing molecules. KEYWORDS: 3D-structure;Direct protein sequencing;Methyltransferase;S-adenosyl-L-methionine;Transferase " P94544,"PROTEIN NAMES: DNA polymerase/3'-5' exonuclease PolX [Includes: DNA polymerase type-X ; 3'-5' exodeoxyribonuclease (3'-5' exonuclease) ] PROTEIN FAMILY: DNA polymerase type-X family; PHP family ORGANISM: Bacillus subtilis (strain 168) SIMILARITY: In the N-terminal section; belongs to the DNA polymerase type-X family.; SIMILARITY: In the C-terminal section; belongs to the PHP family. FUNCTION: Strictly DNA-template-directed DNA polymerase, preferentially acting on DNA structures containing gaps from one to a few nucleotides and bearing a phosphate group at the 5' end of the downstream DNA. The fact that PolX is able to conduct filling of a single-nucleotide gap, allowing further sealing of the resulting nick by a DNA ligase, points to a putative role in base excision repair (BER) during the B.subtilis life cycle. Moreover, also possesses a 3'-5' exonuclease activity able to edit unpaired 3'-termini in a gapped DNA substrate and likely involved in resecting unannealed 3'-termini during DNA repair. The same PolX molecule could perform the subsequent gap-filling step. Does not display 5'-deoxyribose 5'-phosphate (dRP) lyase activity, as predicted by the lack of the lysine and tyrosine residues responsible for the dRP lyase activity in some other PolX members. KEYWORDS: DNA damage;DNA repair;DNA replication;DNA synthesis;DNA-binding;DNA-directed DNA polymerase;Hydrolase;Magnesium;Manganese;Metal-binding;Multifunctional enzyme;Nucleotidyltransferase;Reference proteome;Transferase " P95125,"PROTEIN NAMES: Carboxylic ester hydrolase LipN PROTEIN FAMILY: 'GDXG' lipolytic enzyme family ORGANISM: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) SIMILARITY: Belongs to the 'GDXG' lipolytic enzyme family. FUNCTION: Non specific carboxylic ester hydrolase. Hydrolyzes various pNP-esters, with a preference for short carbon chain substrates. Can also hydrolyze tributyrin to di- and monobutyrin and 4-hydroxyphenylacetate to hydroquinone. KEYWORDS: Cytoplasm;Hydrolase;Reference proteome SUBCELLULAR LOCATION: Cytoplasm " P95470,"PROTEIN NAMES: Extracellular exo-alpha-(1->5)-L-arabinofuranosidase ArbA (ABF) (Extracellular arabinan exo-alpha-(1->5)-L-arabinosidase ArbA) (Arabinosidase) PROTEIN FAMILY: Glycosyl hydrolase 43 family ORGANISM: Cellvibrio japonicus (strain Ueda107) (Pseudomonas fluorescens subsp. cellulosa) SIMILARITY: Belongs to the glycosyl hydrolase 43 family. FUNCTION: Involved in the degradation of arabinan and is a key enzyme in the complete degradation of the plant cell wall. Catalyzes the cleavage of the terminal alpha-(1->5)-arabinofuranosyl bonds of linear arabinan and carboxymethylarabinan to produce almost exclusively arabinotriose. PATHWAY: Glycan metabolism; L-arabinan degradation. KEYWORDS: 3D-structure;Calcium;Carbohydrate metabolism;Direct protein sequencing;Glycosidase;Hydrolase;Metal-binding;Secreted;Signal SUBCELLULAR LOCATION: Secreted MISCELLANEOUS: The relative activity of the enzyme against the arabino-oligosaccharides increases with the increasing size of the substrate up to arabinohexaose, after which the rate remains constant. The substrate-binding site accommodates six arabinose units, with cleavage occurring between binding sites 3 and 4." P95483,"PROTEIN NAMES: Aminopyrrolnitrin oxygenase PrnD (Arylamine oxygenase) ORGANISM: Pseudomonas fluorescens FUNCTION: Involved in the biosynthesis of the antifungal antibiotic pyrrolnitrin (PRN). Catalyzes the oxidation of the amino group of aminopyrrolnitrin (APRN) to a nitro group to form PRN. It has high substrate specificity toward physiological substrate aminopyrrolnitrin, p-aminobenzylamine (pABA), p-aminobenzyl alcohol, and p-aminophenyl alanine. PATHWAY: Antibiotic biosynthesis. KEYWORDS: 2Fe-2S;Antibiotic biosynthesis;Flavoprotein;FMN;Iron;Iron-sulfur;Metal-binding;NADP;Oxidoreductase " P95619,"PROTEIN NAMES: Acyclic carotenoid 1,2-hydratase (1-hydroxyneurosporene hydratase) (Hydroxylycopene hydratase) (Hydroxyneurosporene synthase) (Lycopene hydratase) (Neurosporene hydratase) PROTEIN FAMILY: CrtC hydratase family ORGANISM: Rubrivivax gelatinosus (Rhodocyclus gelatinosus) (Rhodopseudomonas gelatinosa) SIMILARITY: Belongs to the CrtC hydratase family. FUNCTION: Involved in the biosynthesis of carotenoids spheroidene and spirilloxanthin. Catalyzes the hydration of neurosporene to the corresponding hydroxylated carotenoids 1-HO-neurosporene and 1,1'-(HO)2-neurosporene and that of lycopene to 1-HO-lycopene and 1,1'-(HO)2-lycopene. Can also act on demethylspheroidene, spheroidene, 1-HO-3,4-didehydrolycopene and geranylgeraniol. PATHWAY: Carotenoid biosynthesis; spheroidene biosynthesis. KEYWORDS: Carotenoid biosynthesis;Cell membrane;Chlorophyll biosynthesis;Lyase;Membrane;Photosynthesis SUBCELLULAR LOCATION: Cell membrane " P95727,"PROTEIN NAMES: 1-cyclohexenylcarbonyl-CoA reductase (Cyclohexane-1-carbonyl-CoA reductase (NADP(+))) PROTEIN FAMILY: Short-chain dehydrogenases/reductases (SDR) family ORGANISM: Streptomyces collinus SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FUNCTION: Involved in the biosynthesis of the antifungal antibiotic ansatrienin A (mycotrienin I). Catalyzes three of the reductive steps involved in the formation of the cyclohexanecarboxylic acid (CHC) moiety of ansatrienin from shikimic acid. Can use 3,4-dihydroxycyclohexa-1,5-diene-1-carbonyl-CoA, 5-hydroxycyclohex-1-ene-1-carbonyl-CoA and cyclohex-1-ene-1-carbonyl-CoA as substrates. PATHWAY: Antibiotic biosynthesis. KEYWORDS: Antibiotic biosynthesis;Direct protein sequencing;NADP;Oxidoreductase " P96022,"PROTEIN NAMES: DNA polymerase IV (Pol IV) PROTEIN FAMILY: DNA polymerase type-Y family ORGANISM: Saccharolobus solfataricus (Sulfolobus solfataricus) SIMILARITY: Belongs to the DNA polymerase type-Y family. FUNCTION: Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by PolIV. Exhibits no 3'-5' exonuclease (proofreading) activity. May be involved in translesional synthesis. KEYWORDS: 3D-structure;Cytoplasm;DNA damage;DNA repair;DNA replication;DNA-binding;DNA-directed DNA polymerase;Magnesium;Metal-binding;Mutator protein;Nucleotidyltransferase;Transferase SUBCELLULAR LOCATION: Cytoplasm " P96155,"PROTEIN NAMES: Beta-hexosaminidase (Beta-N-acetylhexosaminidase) (N-acetyl-beta-glucosaminidase) PROTEIN FAMILY: Glycosyl hydrolase 20 family ORGANISM: Vibrio furnissii SIMILARITY: Belongs to the glycosyl hydrolase 20 family. FUNCTION: Hydrolyzes aryl-N-acetyl-beta-D-glucosaminide (aryl-beta-GlcNAc), aryl-beta-GalNAc and chitin oligosaccharides. Can hydrolyze rapidly the artificial substrates p-nitrophenyl-N-acetyl-beta-D-glucosaminide (PNP-beta-GlcNAc) and 4-methylumbelliferyl-beta-GlcNAc, and is slightly active on p-nitrophenyl-beta-GalNAc. This enzyme is not processive, i.e. when it hydrolyzes (GlcNAc)n, both products, (Glc-NAc)n-1 and the terminal GlcNAc, are released before the enzyme attacks a second molecule of (GlcNAc)n or (GlcNAc)n-1. PATHWAY: Glycan degradation; chitin degradation. KEYWORDS: Carbohydrate metabolism;Chitin degradation;Direct protein sequencing;Glycosidase;Hydrolase;Periplasm;Polysaccharide degradation SUBCELLULAR LOCATION: Periplasm " P96264,"PROTEIN NAMES: Probable lipoprotein aminopeptidase LpqL (Leucine aminopeptidase) (Lipoprotein LpqL) PROTEIN FAMILY: Peptidase M28 family, M28A subfamily ORGANISM: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) SIMILARITY: Belongs to the peptidase M28 family. M28A subfamily. FUNCTION: An aminopeptidase; acts on free N-terminal amino groups with a very strong preference for Leu in the first position. KEYWORDS: Aminopeptidase;Cell membrane;Hydrolase;Lipoprotein;Membrane;Metal-binding;Palmitate;Protease;Reference proteome;Signal;Zinc SUBCELLULAR LOCATION: Cell membrane ; Lipid-anchor " P96589,PROTEIN NAMES: Potassium transporter KimA (K(+) importer A) (Potassium-proton symporter KimA) PROTEIN FAMILY: Amino acid-polyamine-organocation (APC) superfamily ORGANISM: Bacillus subtilis (strain 168) SIMILARITY: Belongs to the amino acid-polyamine-organocation (APC) superfamily. FUNCTION: High-affinity potassium transporter. Functions as a K(+)/H(+) symporter. KEYWORDS: 3D-structure;Cell membrane;Ion transport;Membrane;Metal-binding;Potassium;Potassium transport;Reference proteome;Symport;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein P97084,"PROTEIN NAMES: Threonine-phosphate decarboxylase (L-threonine-O-3-phosphate decarboxylase) PROTEIN FAMILY: Class-II pyridoxal-phosphate-dependent aminotransferase family ORGANISM: Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) SIMILARITY: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. FUNCTION: Decarboxylates L-threonine-O-3-phosphate to yield (R)-1-amino-2-propanol O-2-phosphate, the precursor for the linkage between the nucleotide loop and the corrin ring in cobalamin. PATHWAY: Cofactor biosynthesis; adenosylcobalamin biosynthesis. KEYWORDS: 3D-structure;Cobalamin biosynthesis;Lyase;Pyridoxal phosphate;Reference proteome " P97290,"PROTEIN NAMES: Plasma protease C1 inhibitor (C1 Inh) (C1Inh) (C1 esterase inhibitor) (C1-inhibiting factor) (Serpin G1) PROTEIN FAMILY: Serpin family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the serpin family. FUNCTION: Activation of the C1 complex is under control of the C1-inhibitor. It forms a proteolytically inactive stoichiometric complex with the C1r or C1s proteases. May play a potentially crucial role in regulating important physiological pathways including complement activation, blood coagulation, fibrinolysis and the generation of kinins. Very efficient inhibitor of FXIIa. May inhibit chymotrypsin and kallikrein. KEYWORDS: Blood coagulation;Complement pathway;Disulfide bond;Fibrinolysis;Glycoprotein;Hemostasis;Immunity;Innate immunity;Protease inhibitor;Reference proteome;Secreted;Serine protease inhibitor;Signal SUBCELLULAR LOCATION: Secreted. " P97292,PROTEIN NAMES: Histamine H2 receptor (H2R) (HH2R) (Gastric receptor I) PROTEIN FAMILY: G-protein coupled receptor 1 family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the G-protein coupled receptor 1 family. FUNCTION: The H2 subclass of histamine receptors mediates gastric acid secretion. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. KEYWORDS: Cell membrane;Disulfide bond;G-protein coupled receptor;Glycoprotein;Lipoprotein;Membrane;Palmitate;Receptor;Reference proteome;Transducer;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. P97361,"PROTEIN NAMES: BPI fold-containing family A member 1 (Palate lung and nasal epithelium clone protein) PROTEIN FAMILY: BPI/LBP/Plunc superfamily, Plunc family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the BPI/LBP/Plunc superfamily. Plunc family. FUNCTION: Lipid-binding protein which shows high specificity for the surfactant phospholipid dipalmitoylphosphatidylcholine (DPPC) (By similarity). Plays a role in the innate immune responses of the upper airways. Reduces the surface tension in secretions from airway epithelia and inhibits the formation of biofilm by pathogenic Gram-negative bacteria, such as P.aeruginosa and K.pneumoniae. Negatively regulates proteolytic cleavage of SCNN1G, an event that is required for activation of the epithelial sodium channel (ENaC), and thereby contributes to airway surface liquid homeostasis and proper clearance of mucus (By similarity). Plays a role in the airway inflammatory response after exposure to irritants (By similarity). May attract macrophages and neutrophils (By similarity). KEYWORDS: 3D-structure;Antibiotic;Antimicrobial;Disulfide bond;Glycoprotein;Immunity;Innate immunity;Lipid-binding;Reference proteome;Repeat;Secreted;Signal SUBCELLULAR LOCATION: Secreted Note=Apical side of airway epithelial cells. Detected in airway surface liquid, nasal mucus and sputum. " P97436,"PROTEIN NAMES: Homeobox protein Nkx-3.1 (Homeobox protein NK-3 homolog A) PROTEIN FAMILY: NK-3 homeobox family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the NK-3 homeobox family. FUNCTION: Transcription factor, which binds preferentially the consensus sequence 5'-TAAGT[AG]-3' and can behave as a transcriptional repressor (By similarity). Plays an important role in normal prostate development, regulating proliferation of glandular epithelium and in the formation of ducts in prostate. Acts as a tumor suppressor controlling prostate carcinogenesis, as shown by the ability to suppress growth and tumorigenicity of prostate carcinoma cells. Plays a role in the formation of minor salivary glands (particularly palatine and lingual glands). KEYWORDS: Developmental protein;DNA-binding;Homeobox;Nucleus;Reference proteome;Repressor;Transcription;Transcription regulation;Tumor suppressor;Ubl conjugation SUBCELLULAR LOCATION: Nucleus " P97481,PROTEIN NAMES: Endothelial PAS domain-containing protein 1 (EPAS-1) (HIF-1-alpha-like factor) (HLF) (mHLF) (HIF-related factor) (HRF) (Hypoxia-inducible factor 2-alpha) (HIF-2-alpha) (HIF2-alpha) ORGANISM: Mus musculus (Mouse) FUNCTION: Transcription factor involved in the induction of oxygen regulated genes. Heterodimerizes with ARNT; heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation requires recruitment of transcriptional coactivators such as CREBBP and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD (By similarity). KEYWORDS: 3D-structure;Activator;Angiogenesis;Developmental protein;Differentiation;Direct protein sequencing;DNA-binding;Hydroxylation;Nucleus;Phosphoprotein;Reference proteome;Repeat;Transcription;Transcription regulation;Ubl conjugation SUBCELLULAR LOCATION: Nucleus Nucleus speckle Note=Colocalizes with HIF3A isoform 2 in the nucleus and speckles. P97544,"PROTEIN NAMES: Phospholipid phosphatase 3 (Differentially expressed in rat intestine 42) (Dri42) (Lipid phosphate phosphohydrolase 3) (PAP2-beta) (Phosphatidate phosphohydrolase type 2b) (Phosphatidic acid phosphatase 2b) (PAP-2b) (PAP2b) PROTEIN FAMILY: PA-phosphatase related phosphoesterase family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the PA-phosphatase related phosphoesterase family. FUNCTION: Magnesium-independent phospholipid phosphatase of the plasma membrane that catalyzes the dephosphorylation of a variety of glycerolipid and sphingolipid phosphate esters including phosphatidate/PA, lysophosphatidate/LPA, diacylglycerol pyrophosphate/DGPP, sphingosine 1-phosphate/S1P and ceramide 1-phosphate/C1P. Also acts on N-oleoyl ethanolamine phosphate/N-(9Z-octadecenoyl)-ethanolamine phosphate, a potential physiological compound. Has both an extracellular and an intracellular phosphatase activity, allowing the hydrolysis and the cellular uptake of these bioactive lipid mediators from the milieu, regulating signal transduction in different cellular processes. Through the dephosphorylation of extracellular sphingosine-1-phosphate and the regulation of its extra- and intracellular availability, plays a role in vascular homeostasis, regulating endothelial cell migration, adhesion, survival, proliferation and the production of pro-inflammatory cytokines (By similarity). By maintaining the appropriate levels of this lipid in the cerebellum, also ensure its proper development and function (By similarity). Through its intracellular lipid phosphatase activity may act in early compartments of the secretory pathway, regulating the formation of Golgi to endoplasmic reticulum retrograde transport carriers (By similarity).; FUNCTION: Independently of this phosphatase activity may also function in the Wnt signaling pathway and the stabilization of beta-catenin/CTNNB1, thereby regulating cell proliferation, migration and differentiation in angiogenesis or yet in tumor growth. Also plays a role in integrin-mediated cell-cell adhesion in angiogenesis. PATHWAY: Lipid metabolism; phospholipid metabolism. KEYWORDS: Cell membrane;Endoplasmic reticulum;Glycoprotein;Golgi apparatus;Hydrolase;Lipid metabolism;Membrane;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Basolateral cell membrane ; Multi-pass membrane protein Endoplasmic reticulum membrane ; Multi-pass membrane protein Endoplasmic reticulum-Golgi intermediate compartment membrane ; Multi-pass membrane protein Golgi apparatus membrane ; Multi-pass membrane protein Golgi apparatus, trans-Golgi network membrane ; Multi-pass membrane protein Membrane raft ; Multi-pass membrane protein Note=Cycles between the endoplasmic reticulum and the Golgi. " P97564,"PROTEIN NAMES: Glycerol-3-phosphate acyltransferase 1, mitochondrial (GPAT-1) PROTEIN FAMILY: GPAT/DAPAT family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the GPAT/DAPAT family. FUNCTION: Esterifies acyl-group from acyl-ACP to the sn-1 position of glycerol-3-phosphate, an essential step in glycerolipids biosynthesis such as triglycerides, phosphatidic acids and lysophosphatidic acids. PATHWAY: Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3. KEYWORDS: Acetylation;Acyltransferase;Lipid biosynthesis;Lipid metabolism;Membrane;Mitochondrion;Mitochondrion outer membrane;Phospholipid biosynthesis;Phospholipid metabolism;Phosphoprotein;Reference proteome;Transferase;Transit peptide;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Mitochondrion outer membrane ; Multi-pass membrane protein " P97577,"PROTEIN NAMES: Fasciculation and elongation protein zeta-1 (Zygin I) (Zygin-1) PROTEIN FAMILY: Zygin family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the zygin family. FUNCTION: May be involved in axonal outgrowth as component of the network of molecules that regulate cellular morphology and axon guidance machinery. May participate in the transport of mitochondria and other cargos along microtubules. KEYWORDS: Cell membrane;Coiled coil;Cytoplasm;Cytoskeleton;Membrane;Microtubule;Phosphoprotein;Reference proteome;Transport;Ubl conjugation SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Cell membrane. Note=Colocalizes with both, alpha- and gamma-tubulin (By similarity). Translocated from the plasma membrane to the cytoplasm by activation of the PKC zeta. " P97610,"PROTEIN NAMES: Synaptotagmin-12 (Synaptotagmin XII) (SytXII) (Synaptotagmin-related gene 1 protein) (Srg1) PROTEIN FAMILY: Synaptotagmin family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the synaptotagmin family. FUNCTION: Synaptic vesicle phosphoprotein that enhances spontaneous neurotransmitter release but does not effect induced neurotransmitter release. Unlike other synaptotagmins, it does not bind Ca(2+) or phospholipids. Essential for mossy-fiber long-term potentiation in the hippocampus (By similarity). KEYWORDS: Cytoplasmic vesicle;Membrane;Phosphoprotein;Reference proteome;Repeat;Synapse;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane ; Single-pass membrane protein " P97636,"PROTEIN NAMES: Interleukin-18 (IL-18) (Interferon gamma-inducing factor) (IFN-gamma-inducing factor) (Interleukin-1 gamma) (IL-1 gamma) PROTEIN FAMILY: IL-1 family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the IL-1 family. FUNCTION: Pro-inflammatory cytokine primarily involved in epithelial barrier repair, polarized T-helper 1 (Th1) cell and natural killer (NK) cell immune responses. Upon binding to IL18R1 and IL18RAP, forms a signaling ternary complex which activates NF-kappa-B, triggering synthesis of inflammatory mediators. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells and natural killer (NK) cells. Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore. KEYWORDS: Alternative splicing;Cytokine;Cytoplasm;Inflammatory response;Reference proteome;Secreted SUBCELLULAR LOCATION: Cytoplasm, cytosol Secreted Note=The precursor is cytosolic. In response to inflammasome-activating signals, cleaved and secreted. Mature form is secreted and released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore. In contrast, the precursor form is not released, due to the presence of an acidic region that is proteolytically removed by CASP1 during maturation. The secretion is dependent on protein unfolding and facilitated by the cargo receptor TMED10. " P97797,"PROTEIN NAMES: Tyrosine-protein phosphatase non-receptor type substrate 1 (SHP substrate 1) (SHPS-1) (Brain Ig-like molecule with tyrosine-based activation motifs) (Bit) (CD172 antigen-like family member A) (Inhibitory receptor SHPS-1) (MyD-1 antigen) (Signal-regulatory protein alpha-1) (Sirp-alpha-1) (mSIRP-alpha1) (p84) (CD antigen CD172a) ORGANISM: Mus musculus (Mouse) FUNCTION: Immunoglobulin-like cell surface receptor for CD47. Acts as a docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function. Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells (By similarity). Plays a role in antiviral immunity and limits new world arenavirus infection by decreasing virus internalization. Receptor for THBS1 (By similarity). Interaction with THBS1 stimulates phosphorylation of SIRPA (By similarity). In response to THBS1, involved in ROS signaling in non-phagocytic cells, stimulating NADPH oxidase-derived ROS production (By similarity). KEYWORDS: 3D-structure;Alternative splicing;Direct protein sequencing;Disulfide bond;Glycoprotein;Immunoglobulin domain;Membrane;Phosphoprotein;Reference proteome;Repeat;SH3-binding;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. " P97849,"PROTEIN NAMES: Long-chain fatty acid transport protein 1 (Arachidonate--CoA ligase) (Fatty acid transport protein) (Fatty acid transport protein 1) (FATP-1) (Long-chain-fatty-acid--CoA ligase) (Solute carrier family 27 member 1) (Very long-chain acyl-CoA synthetase) PROTEIN FAMILY: ATP-dependent AMP-binding enzyme family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the ATP-dependent AMP-binding enzyme family. FUNCTION: Mediates the import of long-chain fatty acids (LCFA) into the cell by facilitating their transport at the plasma membrane. Also functions as an acyl-CoA ligase catalyzing the ATP-dependent formation of fatty acyl-CoA using LCFA and very-long-chain fatty acids (VLCFA) as substrates, which prevents fatty acid efflux from cells and might drive more fatty acid uptake. May act directly as a bona fide transporter, or alternatively, in a cytoplasmic or membrane-associated multimeric protein complex to trap and draw fatty acids towards accumulation. Plays a pivotal role in regulating available LCFA substrates from exogenous sources in tissues undergoing high levels of beta-oxidation or triglyceride synthesis. May be involved in regulation of cholesterol metabolism. Probably involved in fatty acid transport across the blood barrier (By similarity). KEYWORDS: Cell membrane;Cytoplasm;Fatty acid metabolism;Ligase;Lipid metabolism;Lipid transport;Membrane;Nucleotide-binding;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane ; Single-pass membrane protein Endomembrane system ; Single-pass membrane protein Cytoplasm Note=Plasma membrane and intracellular membranes, at least in adipocytes. In adipocytes, but not myocytes, insulin via the mTORC1 signaling pathway induces a rapid translocation of SLC27A1 from intracellular compartments to the plasma membrane, paralleled by increased LCFA uptake. Insulin-dependent translocation from the cytoplasm to the cell membrane is regulated by EPRS1. Predominantly cytoplasmic in myocytes. " P97873,"PROTEIN NAMES: Lysyl oxidase homolog 1 (Lysyl oxidase 2) (Lysyl oxidase-like protein 1) PROTEIN FAMILY: Lysyl oxidase family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the lysyl oxidase family. FUNCTION: Catalyzes the oxidative deamination of lysine and hydroxylysine residues in collagen and elastin, resulting in the formation of covalent cross-linkages, and the stabilization of collagen and elastin fibers. Essential for the elastic fiber homeostasis and for their maintenance at adult age. KEYWORDS: Cleavage on pair of basic residues;Copper;Disulfide bond;Extracellular matrix;LTQ;Metal-binding;Oxidoreductase;Reference proteome;Secreted;Signal;TPQ SUBCELLULAR LOCATION: Secreted, extracellular space Secreted, extracellular space, extracellular matrix " P98004,"PROTEIN NAMES: Quinol oxidase subunit 1 (Cytochrome aa3 subunit 1) (Oxidase aa(3) subunit 1) (Quinol oxidase polypeptide I) PROTEIN FAMILY: Heme-copper respiratory oxidase family ORGANISM: Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770) SIMILARITY: Belongs to the heme-copper respiratory oxidase family. FUNCTION: Catalyzes the reduction of oxygen to water.; FUNCTION: Subunits I, II and III form the functional core of the enzyme complex. Electrons originating in caldariella quinol are transferred to the binuclear center formed by heme A3 and Cu(B).; FUNCTION: Subunit I binds heme a and the bimetallic center. KEYWORDS: Cell membrane;Copper;Electron transport;Heme;Iron;Membrane;Metal-binding;Oxidoreductase;Reference proteome;Respiratory chain;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. " P98008,PROTEIN NAMES: Nitric oxide reductase subunit B (NOR large subunit) (Nitric oxide reductase cytochrome b subunit) PROTEIN FAMILY: Heme-copper respiratory oxidase family ORGANISM: Stutzerimonas stutzeri (Pseudomonas stutzeri) SIMILARITY: Belongs to the heme-copper respiratory oxidase family. FUNCTION: Component of the anaerobic respiratory chain that transforms nitrate to dinitrogen (denitrification). NorB is the catalytic subunit of the enzyme complex. Shows proton pump activity across the membrane in denitrifying bacterial cells. The mononitrogen reduction is probably coupled to electron transport phosphorylation. PATHWAY: Nitrogen metabolism; nitrate reduction (denitrification); dinitrogen from nitrate: step 3/4. KEYWORDS: Cell membrane;Direct protein sequencing;Electron transport;Heme;Iron;Membrane;Metal-binding;Oxidoreductase;Respiratory chain;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein P98064,"PROTEIN NAMES: Mannan-binding lectin serine protease 1 (Complement factor MASP-3) (Complement-activating component of Ra-reactive factor) (Mannose-binding lectin-associated serine protease 1) (MASP-1) (Mannose-binding protein-associated serine protease) (Ra-reactive factor serine protease p100) (RaRF) (Serine protease 5) [Cleaved into: Mannan-binding lectin serine protease 1 heavy chain; Mannan-binding lectin serine protease 1 light chain] PROTEIN FAMILY: Peptidase S1 family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the peptidase S1 family. FUNCTION: Functions in the lectin pathway of complement, which performs a key role in innate immunity by recognizing pathogens through patterns of sugar moieties and neutralizing them. The lectin pathway is triggered upon binding of mannan-binding lectin (MBL) and ficolins to sugar moieties which leads to activation of the associated proteases MASP1 and MASP2. Functions as an endopeptidase and may activate MASP2 or C2 or directly activate C3 the key component of complement reaction. Isoform 2 may have an inhibitory effect on the activation of the lectin pathway of complement or may cleave IGFBP5. Also plays a role in development. KEYWORDS: Alternative splicing;Autocatalytic cleavage;Calcium;Complement activation lectin pathway;Direct protein sequencing;Disulfide bond;EGF-like domain;Glycoprotein;Hydrolase;Hydroxylation;Immunity;Innate immunity;Metal-binding;Protease;Reference proteome;Repeat;Secreted;Serine protease;Signal;Sushi SUBCELLULAR LOCATION: Secreted MISCELLANEOUS: [Isoform 2]: Contains a N-linked (GlcNAc..) asparagine at position 538 Contains a N-linked (GlcNAc..) asparagine at position 604." P98152,"PROTEIN NAMES: Transcription factor p65 (Nuclear factor NF-kappa-B p65 subunit) ORGANISM: Gallus gallus (Chicken) FUNCTION: NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. RELA shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. KEYWORDS: Activator;Cytoplasm;DNA-binding;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus Cytoplasm Note=Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B). " Q00056,"PROTEIN NAMES: Homeobox protein Hox-A4 (Homeobox protein Hox-1.4) (Homeobox protein Hox-1D) PROTEIN FAMILY: Antp homeobox family, Deformed subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the Antp homeobox family. Deformed subfamily. FUNCTION: Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Binds to sites in the 5'-flanking sequence of its coding region with various affinities. The consensus sequences of the high and low affinity binding sites are 5'-TAATGA[CG]-3' and 5'-CTAATTTT-3'. KEYWORDS: Developmental protein;DNA-binding;Homeobox;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus. " Q00246,"PROTEIN NAMES: GTP-binding protein RHO4 PROTEIN FAMILY: Small GTPase superfamily, Rho family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the small GTPase superfamily. Rho family. FUNCTION: Plays an important role in cell growth. Required to keep the uninucleated state. May be involved in the organization of the cytoskeleton which affects microtubule functions. Most likely RHO3 and RHO4 of S.cerevisiae regulate partially overlapping but different pathways. KEYWORDS: Cell membrane;Direct protein sequencing;GTP-binding;Hydrolase;Lipoprotein;Membrane;Methylation;Nucleotide-binding;Phosphoprotein;Prenylation;Reference proteome SUBCELLULAR LOCATION: Cell membrane ; Lipid-anchor ; Cytoplasmic side " Q00362,"PROTEIN NAMES: Protein phosphatase PP2A regulatory subunit B (Cell division control protein 55) (PR55) PROTEIN FAMILY: Phosphatase 2A regulatory subunit B family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the phosphatase 2A regulatory subunit B family. FUNCTION: Phosphatase 2A affects a variety of biological processes in the cell such as transcription, cell cycle progression and cellular morphogenesis, and provides an initial identification of critical substrates for this phosphatase. The regulatory subunit may direct the catalytic subunit to distinct, albeit overlapping, subsets of substrates. KEYWORDS: Cell cycle;Phosphoprotein;Reference proteome;Repeat;WD repeat MISCELLANEOUS: Present with 8600 molecules/cell in log phase SD medium." Q00366,"PROTEIN NAMES: Cellular tumor antigen p53 (Tumor suppressor p53) PROTEIN FAMILY: P53 family ORGANISM: Mesocricetus auratus (Golden hamster) SIMILARITY: Belongs to the p53 family. FUNCTION: Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Its pro-apoptotic activity is activated via its interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2 (By similarity). However, this activity is inhibited when the interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2 is displaced by PPP1R13L/iASPP (By similarity). In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seems to have an effect on cell-cycle regulation. Regulates the circadian clock by repressing CLOCK-BMAL1-mediated transcriptional activation of PER2. KEYWORDS: Acetylation;Activator;Apoptosis;Biological rhythms;Cell cycle;Cytoplasm;Cytoskeleton;DNA-binding;Endoplasmic reticulum;Isopeptide bond;Metal-binding;Methylation;Mitochondrion;Necrosis;Nucleus;Phosphoprotein;Reference proteome;Repressor;Transcription;Transcription regulation;Tumor suppressor;Ubl conjugation;Zinc SUBCELLULAR LOCATION: Cytoplasm Nucleus Nucleus, PML body Endoplasmic reticulum Mitochondrion matrix Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Note=Interaction with BANP promotes nuclear localization. Recruited into PML bodies together with CHEK2. Translocates to mitochondria upon oxidative stress. Translocates to mitochondria in response to mitomycin C treatment (By similarity). Competitive inhibition of TP53 interaction with HSPA9/MOT-2 by UBXN2A results in increased protein abundance and subsequent translocation of TP53 to the nucleus (By similarity). " Q00422,PROTEIN NAMES: GA-binding protein alpha chain (GABP subunit alpha) PROTEIN FAMILY: ETS family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the ETS family. FUNCTION: Transcription factor capable of interacting with purine rich repeats (GA repeats). Positively regulates transcription of transcriptional repressor Rhit/Zpf13. KEYWORDS: 3D-structure;DNA-binding;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus. Q00456,"PROTEIN NAMES: Toluene-4-monooxygenase system, hydroxylase component subunit alpha (T4MO) (Toluene-4-monooxygenase hydroxylase subunit) (T4moH) (Toluene-4-monooxygenase system protein A) (T4moA) PROTEIN FAMILY: TmoA/XamoA family ORGANISM: Pseudomonas mendocina SIMILARITY: Belongs to the TmoA/XamoA family. FUNCTION: Component of the toluene-4-monooxygenase multicomponent enzyme system which catalyzes the O2- and NADH-dependent hydroxylation of toluene to form p-cresol. Also able to convert benzene to phenol, catechol, and 1,2,3-trihydroxybenzene by successive hydroxylations. PATHWAY: Xenobiotic degradation; toluene degradation. KEYWORDS: 3D-structure;Aromatic hydrocarbons catabolism;Direct protein sequencing;Iron;Metal-binding;Monooxygenase;NAD;Oxidoreductase " Q00539,"PROTEIN NAMES: Protein NAM8 (Nuclear accommodation of mitochondria protein 8) (U1 snRNP component NAM8) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Component of the U1 small nuclear ribonucleoprotein complex (U1 snRNP) involved in the initiation of meiotic recombination. Involved in the formation of DSBs at recombination hot-spots through meiosis-specific splicing of REC107 pre-mRNA. Collaborates with MER1 to promote splicing of essential meiotic mRNAs REC10, AMA1, MER3, HFM1, SPO22 and PCH2. NAM8 interacts with the pre-mRNA downstream of the 5' splice site, in a region of non-conserved sequence and is required for efficient splicing of uncapped RNA precursor. KEYWORDS: 3D-structure;mRNA processing;mRNA splicing;Reference proteome;Repeat;RNA-binding;Spliceosome MISCELLANEOUS: Present with 1480 molecules/cell in log phase SD medium." Q00613,"PROTEIN NAMES: Heat shock factor protein 1 (HSF 1) (Heat shock transcription factor 1) (HSTF 1) PROTEIN FAMILY: HSF family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the HSF family. FUNCTION: Functions as a stress-inducible and DNA-binding transcription factor that plays a central role in the transcriptional activation of the heat shock response (HSR), leading to the expression of a large class of molecular chaperones, heat shock proteins (HSPs), that protect cells from cellular insult damage. In unstressed cells, is present in a HSP90-containing multichaperone complex that maintains it in a non-DNA-binding inactivated monomeric form. Upon exposure to heat and other stress stimuli, undergoes homotrimerization and activates HSP gene transcription through binding to site-specific heat shock elements (HSEs) present in the promoter regions of HSP genes. Upon heat shock stress, forms a chromatin-associated complex with TTC5/STRAP and p300/EP300 to stimulate HSR transcription, therefore increasing cell survival. Activation is reversible, and during the attenuation and recovery phase period of the HSR, returns to its unactivated form. Binds to inverted 5'-NGAAN-3' pentamer DNA sequences. Binds to chromatin at heat shock gene promoters. Activates transcription of transcription factor FOXR1 which in turn activates transcription of the heat shock chaperones HSPA1A and HSPA6 and the antioxidant NADPH-dependent reductase DHRS2. Also serves several other functions independently of its transcriptional activity. Involved in the repression of Ras-induced transcriptional activation of the c-fos gene in heat-stressed cells. Positively regulates pre-mRNA 3'-end processing and polyadenylation of HSP70 mRNA upon heat-stressed cells in a symplekin (SYMPK)-dependent manner. Plays a role in nuclear export of stress-induced HSP70 mRNA. Plays a role in the regulation of mitotic progression. Also plays a role as a negative regulator of non-homologous end joining (NHEJ) repair activity in a DNA damage-dependent manner. Involved in stress-induced cancer cell proliferation in a IER5-dependent manner.; FUNCTION: (Microbial infection) Plays a role in latent human immunodeficiency virus (HIV-1) transcriptional reactivation. Binds to the HIV-1 long terminal repeat promoter (LTR) to reactivate viral transcription by recruiting cellular transcriptional elongation factors, such as CDK9, CCNT1 and EP300. KEYWORDS: 3D-structure;Acetylation;Activator;Alternative splicing;Centromere;Chromosome;Cytoplasm;Cytoskeleton;Direct protein sequencing;DNA damage;DNA repair;DNA-binding;Host-virus interaction;Isopeptide bond;Kinetochore;mRNA processing;mRNA transport;Nucleus;Phosphoprotein;Reference proteome;Stress response;Transcription;Transcription regulation;Transport;Ubl conjugation SUBCELLULAR LOCATION: Nucleus Cytoplasm Nucleus, nucleoplasm Cytoplasm, perinuclear region Cytoplasm, cytoskeleton, spindle pole Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Chromosome, centromere, kinetochore Note=The monomeric form is cytoplasmic in unstressed cells. Predominantly nuclear protein in both unstressed and heat shocked cells. Translocates in the nucleus upon heat shock. Nucleocytoplasmic shuttling protein. Colocalizes with IER5 in the nucleus. Colocalizes with BAG3 to the nucleus upon heat stress. Localizes in subnuclear granules called nuclear stress bodies (nSBs) upon heat shock. Colocalizes with SYMPK and SUMO1 in nSBs upon heat shock. Colocalizes with PRKACA/PKA in the nucleus and nSBs upon heat shock. Relocalizes from the nucleus to the cytoplasm during the attenuation and recovery phase period of the heat shock response. Translocates in the cytoplasm in a YWHAE- and XPO1/CRM1-dependent manner. Together with histone H2AX, redistributed in discrete nuclear DNA damage-induced foci after ionizing radiation (IR). Colocalizes with calcium-responsive transactivator SS18L1 at kinetochore region on the mitotic chromosomes. Colocalizes with gamma tubulin at centrosome. Localizes at spindle pole in metaphase. Colocalizes with PLK1 at spindle poles during prometaphase. " Q00618,"PROTEIN NAMES: Geranylgeranyl transferase type-2 subunit alpha (GGTase-II-alpha) (Geranylgeranyl transferase type II subunit alpha) (PGGT) (Type II protein geranyl-geranyltransferase subunit alpha) (YPT1/SEC4 proteins geranylgeranyltransferase subunit alpha) PROTEIN FAMILY: Protein prenyltransferase subunit alpha family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the protein prenyltransferase subunit alpha family. FUNCTION: Catalyzes the transfer of a geranyl-geranyl moiety from geranyl-geranyl pyrophosphate to proteins having the C-terminal -XCC or -XCXC, where both cysteines may become modified. Acts on YPT1 and SEC4. KEYWORDS: Prenyltransferase;Reference proteome;Repeat;Transferase " Q00684,"PROTEIN NAMES: Tyrosine-protein phosphatase CDC14 PROTEIN FAMILY: Protein-tyrosine phosphatase family, Non-receptor class CDC14 subfamily ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the protein-tyrosine phosphatase family. Non-receptor class CDC14 subfamily. FUNCTION: Protein phosphatase which antagonizes mitotic cyclin-dependent kinase CDC28, the inactivation of which is essential for exit from mitosis. To access its substrates, is released from nucleolar sequestration during mitosis. Plays an essential in coordinating the nuclear division cycle with cytokinesis through the cytokinesis checkpoint. Involved in chromosome segregation, where it is required for meiosis I spindle dissambly as well as for establishing two consecutive chromosome segregation phases. Allows damaged actomyosin rings to be maintained to facilitate completion of cell division in response to minor perturbation of the cell division machinery. Inhibits transcription of ribosomal genes (rDNA) during anaphase and controls segregation of nucleolus by facilitating condensin targeting to rDNA chromatin in anaphase. Dephosphorylates SIC1, a CDC28 inhibitor, and SWI5, a transcription factor for SIC1, and induces degradation of mitotic cyclins, likely by dephosphorylating the activator of mitotic cyclin degradation, CDH1. Dephosphorylates the microtubule bundling factor ASE1 which is required to define a centered and focused mitotic spindle midzone that can drive continuous spindle elongation. Dephosphorylates the anaphase-promoting complex inhibitor ACM1, leading to its degradation. Facilitates INN1-CYK3 complex formation which promotes cytokinesis through the dephosphorylation of CDC28-phosphosphorylated INN1. Reverts also the inhibitory CDC28 phosphorylation of CHS2 for endoplasmic reticulum export, ensuring that septum formation is contingent upon chromosome separation and exit from mitosis. Additional substrates for CDC14 are the formins BNI1 and BNR1, as well as CDC6, DBP2, DSN1, INCENP, KAR9, MCM3, ORC2, ORC6, SLD2, and SWI6. Activity is inhibited by interaction with NET1 which sequesters it to the nucleolus. KEYWORDS: 3D-structure;Cell cycle;Cell division;Cytoplasm;Hydrolase;Meiosis;Mitosis;Nucleus;Phosphoprotein;Protein phosphatase;Reference proteome SUBCELLULAR LOCATION: Nucleus, nucleolus. Cytoplasm. Bud neck. Note=Sequestered in the nucleolus for most of the cell cycle by the nucleolar proteins NET1 and TOF2, and is released into the nucleus and cytoplasm during anaphase. CDC55 maintains CDC14 sequestration in the nucleolus during early meiosis, which is essential for the assembly of the meiosis I spindle. In anaphase, the CDC14 early anaphase release (FEAR) network (including CDC5, ESP1, and SLK19), and the mitotic exit network (including the DBF2-MOB1 complex) coordinately trigger the release of CDC14 from the nucleolus. MISCELLANEOUS: Present with 8550 molecules/cell in log phase SD medium." Q00690,PROTEIN NAMES: E-selectin (CD62 antigen-like family member E) (Endothelial leukocyte adhesion molecule 1) (ELAM-1) (Leukocyte-endothelial cell adhesion molecule 2) (LECAM2) (CD antigen CD62E) PROTEIN FAMILY: Selectin/LECAM family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the selectin/LECAM family. FUNCTION: Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with SELPLG/PSGL1. May have a role in capillary morphogenesis. KEYWORDS: Calcium;Cell adhesion;Cell membrane;Disulfide bond;EGF-like domain;Glycoprotein;Lectin;Membrane;Metal-binding;Reference proteome;Repeat;Signal;Sushi;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein Q00839,"PROTEIN NAMES: Heterogeneous nuclear ribonucleoprotein U (hnRNP U) (GRIP120) (Nuclear p120 ribonucleoprotein) (Scaffold-attachment factor A) (SAF-A) (p120) (pp120) ORGANISM: Homo sapiens (Human) FUNCTION: DNA- and RNA-binding protein involved in several cellular processes such as nuclear chromatin organization, telomere-length regulation, transcription, mRNA alternative splicing and stability, Xist-mediated transcriptional silencing and mitotic cell progression. Plays a role in the regulation of interphase large-scale gene-rich chromatin organization through chromatin-associated RNAs (caRNAs) in a transcription-dependent manner, and thereby maintains genomic stability. Required for the localization of the long non-coding Xist RNA on the inactive chromosome X (Xi) and the subsequent initiation and maintenance of X-linked transcriptional gene silencing during X-inactivation (By similarity). Plays a role as a RNA polymerase II (Pol II) holoenzyme transcription regulator. Promotes transcription initiation by direct association with the core-TFIIH basal transcription factor complex for the assembly of a functional pre-initiation complex with Pol II in a actin-dependent manner. Blocks Pol II transcription elongation activity by inhibiting the C-terminal domain (CTD) phosphorylation of Pol II and dissociates from Pol II pre-initiation complex prior to productive transcription elongation. Positively regulates CBX5-induced transcriptional gene silencing and retention of CBX5 in the nucleus. Negatively regulates glucocorticoid-mediated transcriptional activation. Key regulator of transcription initiation and elongation in embryonic stem cells upon leukemia inhibitory factor (LIF) signaling (By similarity). Involved in the long non-coding RNA H19-mediated Pol II transcriptional repression. Participates in the circadian regulation of the core clock component BMAL1 transcription (By similarity). Plays a role in the regulation of telomere length. Plays a role as a global pre-mRNA alternative splicing modulator by regulating U2 small nuclear ribonucleoprotein (snRNP) biogenesis. Plays a role in mRNA stability. Component of the CRD-mediated complex that promotes MYC mRNA stabilization. Enhances the expression of specific genes, such as tumor necrosis factor TNFA, by regulating mRNA stability, possibly through binding to the 3'-untranslated region (UTR). Plays a role in mitotic cell cycle regulation. Involved in the formation of stable mitotic spindle microtubules (MTs) attachment to kinetochore, spindle organization and chromosome congression. Phosphorylation at Ser-59 by PLK1 is required for chromosome alignement and segregation and progression through mitosis. Contributes also to the targeting of AURKA to mitotic spindle MTs. Binds to double- and single-stranded DNA and RNA, poly(A), poly(C) and poly(G) oligoribonucleotides. Binds to chromatin-associated RNAs (caRNAs). Associates with chromatin to scaffold/matrix attachment region (S/MAR) elements in a chromatin-associated RNAs (caRNAs)-dependent manner. Binds to the Xist RNA. Binds the long non-coding H19 RNA. Binds to SMN1/2 pre-mRNAs at G/U-rich regions. Binds to small nuclear RNAs (snRNAs). Binds to the 3'-UTR of TNFA mRNA. Binds (via RNA-binding RGG-box region) to the long non-coding Xist RNA; this binding is direct and bridges the Xist RNA and the inactive chromosome X (Xi) (By similarity). Also negatively regulates embryonic stem cell differentiation upon LIF signaling (By similarity). Required for embryonic development (By similarity). Binds to brown fat long non-coding RNA 1 (Blnc1); facilitates the recruitment of Blnc1 by ZBTB7B required to drive brown and beige fat development and thermogenesis (By similarity).; FUNCTION: (Microbial infection) Negatively regulates immunodeficiency virus type 1 (HIV-1) replication by preventing the accumulation of viral mRNA transcripts in the cytoplasm. KEYWORDS: Acetylation;Activator;ADP-ribosylation;Alternative splicing;ATP-binding;Biological rhythms;Cell cycle;Cell division;Centromere;Chromatin regulator;Chromosome;Citrullination;Cytoplasm;Cytoskeleton;Developmental protein;Differentiation;Direct protein sequencing;Disease variant;DNA-binding;Epilepsy;Host-virus interaction;Isopeptide bond;Kinetochore;Methylation;Mitosis;mRNA processing;mRNA splicing;Nucleotide-binding;Nucleus;Phosphoprotein;Reference proteome;Repressor;Ribonucleoprotein;RNA-binding;Spliceosome;Transcription;Transcription regulation;Ubl conjugation SUBCELLULAR LOCATION: Nucleus Nucleus matrix Chromosome Nucleus speckle Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Chromosome, centromere, kinetochore Cytoplasm, cytoskeleton, spindle Cytoplasm, cytoskeleton, spindle pole Midbody Cytoplasm Cell surface Cytoplasmic granule Note=Localizes at inactive X chromosome (Xi) regions. Localizes in the nucleus during interphase. At metaphase, localizes with mitotic spindle microtubules (MTs). At anaphase, localizes in the mitotic spindle midzone. Localizes in spindle MTs proximal to spindle poles in a TPX2- and AURKA-dependent manner. The Ser-59 phosphorylated form localizes to centrosomes during prophase and metaphase, to mitotic spindles in anaphase and to the midbody during cytokinesis. Colocalizes with SMARCA4 in the nucleus (By similarity). Colocalizes with CBX5 in the nucleus. Colocalizes with NR3C1 in nuclear speckles. Localized in cytoplasmic ribonucleoprotein (RNP) granules containing untranslated mRNAs. " Q01068,PROTEIN NAMES: Enhancer of split m3 protein (E(spl)m3) (HLH-m3) ORGANISM: Drosophila melanogaster (Fruit fly) FUNCTION: Transcriptional repressor of genes that require a bHLH protein for their transcription. May serve as a transcriptional regulator of the Achaete-scute complex (AS-C) genes. Belongs to notch signaling pathway and depends on Su(H) for transcriptional activation. KEYWORDS: Coiled coil;Developmental protein;Differentiation;DNA-binding;Neurogenesis;Notch signaling pathway;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus Q01080,"PROTEIN NAMES: DNA-directed RNA polymerase I subunit RPA49 (A49) (DNA-directed RNA polymerase I 49 kDa polypeptide) PROTEIN FAMILY: Eukaryotic RPA49/POLR1E RNA polymerase subunit family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the eukaryotic RPA49/POLR1E RNA polymerase subunit family. FUNCTION: DNA-dependent RNA polymerases catalyze the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I (Pol I) which synthesizes ribosomal RNA precursors. Besides, RNA polymerase I has intrinsic RNA cleavage activity. The heterodimer formed by RPA34 and RPA49 stimulates transcript elongation by Pol I. Subunit RPA49 can bind both single-stranded and double-stranded DNA. KEYWORDS: 3D-structure;DNA-binding;DNA-directed RNA polymerase;Nucleotidyltransferase;Nucleus;Phosphoprotein;Reference proteome;Ribosome biogenesis;Transcription;Transferase SUBCELLULAR LOCATION: Nucleus, nucleolus " Q01151,PROTEIN NAMES: CD83 antigen (hCD83) (B-cell activation protein) (Cell surface protein HB15) (CD antigen CD83) ORGANISM: Homo sapiens (Human) FUNCTION: May play a significant role in antigen presentation or the cellular interactions that follow lymphocyte activation. KEYWORDS: 3D-structure;Direct protein sequencing;Disulfide bond;Glycoprotein;Immunoglobulin domain;Membrane;Reference proteome;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. Q01201,"PROTEIN NAMES: Transcription factor RelB (I-Rel) ORGANISM: Homo sapiens (Human) FUNCTION: NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric RelB-p50 and RelB-p52 complexes are transcriptional activators. RELB neither associates with DNA nor with RELA/p65 or REL. Stimulates promoter activity in the presence of NFKB2/p49. As a member of the NUPR1/RELB/IER3 survival pathway, may provide pancreatic ductal adenocarcinoma with remarkable resistance to cell stress, such as starvation or gemcitabine treatment. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-BMAL1 heterodimer in a CRY1/CRY2 independent manner. Increased repression of the heterodimer is seen in the presence of NFKB2/p52. Is required for both T and B lymphocyte maturation and function. KEYWORDS: 3D-structure;Activator;Biological rhythms;Cytoplasm;Cytoskeleton;Disease variant;DNA-binding;Methylation;Nucleus;Phosphoprotein;Reference proteome;Repressor;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Note=Colocalizes with NEK6 in the centrosome. " Q01292,"PROTEIN NAMES: Ketol-acid reductoisomerase, chloroplastic (Acetohydroxy-acid reductoisomerase) (Alpha-keto-beta-hydroxylacyl reductoisomerase) PROTEIN FAMILY: Ketol-acid reductoisomerase family ORGANISM: Spinacia oleracea (Spinach) SIMILARITY: Belongs to the ketol-acid reductoisomerase family. PATHWAY: Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4.; PATHWAY: Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4. KEYWORDS: 3D-structure;Amino-acid biosynthesis;Branched-chain amino acid biosynthesis;Chloroplast;Direct protein sequencing;Magnesium;Metal-binding;NADP;Oxidoreductase;Plastid;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast " Q01433,"PROTEIN NAMES: AMP deaminase 2 (AMP deaminase isoform L) PROTEIN FAMILY: Metallo-dependent hydrolases superfamily, Adenosine and AMP deaminases family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the metallo-dependent hydrolases superfamily. Adenosine and AMP deaminases family. FUNCTION: AMP deaminase plays a critical role in energy metabolism. Catalyzes the deamination of AMP to IMP and plays an important role in the purine nucleotide cycle. PATHWAY: Purine metabolism; IMP biosynthesis via salvage pathway; IMP from AMP: step 1/1. KEYWORDS: 3D-structure;Alternative splicing;Disease variant;Hereditary spastic paraplegia;Hydrolase;Metal-binding;Methylation;Neurodegeneration;Nucleotide metabolism;Phosphoprotein;Reference proteome;Zinc " Q01574,"PROTEIN NAMES: Acetyl-coenzyme A synthetase 1 (Acetate--CoA ligase 1) (Acyl-activating enzyme 1) PROTEIN FAMILY: ATP-dependent AMP-binding enzyme family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the ATP-dependent AMP-binding enzyme family. FUNCTION: Catalyzes the production of acetyl-CoA. Provides the acetyl-CoA source for histone acetylation in the nucleus. 'Aerobic' isozyme of acetyl-coenzyme A synthetase, which supports growth on nonfermentable carbon sources such as glycerol and ethanol. May be required for assimilation of ethanol and acetate. KEYWORDS: 3D-structure;ATP-binding;Cytoplasm;Endoplasmic reticulum;Ligase;Microsome;Mitochondrion;Nucleotide-binding;Nucleus;Reference proteome SUBCELLULAR LOCATION: Microsome Cytoplasm. Mitochondrion. Nucleus. MISCELLANEOUS: Present with 2890 molecules/cell in log phase SD medium." Q01593,PROTEIN NAMES: B3 domain-containing transcription factor ABI3 (Protein ABSCISIC ACID-INSENSITIVE 3) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Participates in abscisic acid-regulated gene expression during seed development. Regulates the transcription of SGR1 and SGR2 that are involved in leaf and embryo degreening. KEYWORDS: Abscisic acid signaling pathway;Activator;Alternative splicing;Cytoplasm;Developmental protein;DNA-binding;Nucleus;Reference proteome;Transcription;Transcription regulation;Ubl conjugation SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=Predominantly found in the nucleus. MISCELLANEOUS: The truncated abi3-6 mutant lacking the DNA-binding domain is unable to localize to the nucleus and is an embryo stay-green mutant.; MISCELLANEOUS: [Isoform 2]: Due to a cryptic intron removal. Q01662,"PROTEIN NAMES: Methionine aminopeptidase 1 (MAP 1) (MetAP 1) (Peptidase M 1) PROTEIN FAMILY: Peptidase M24A family, Methionine aminopeptidase type 1 subfamily ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the peptidase M24A family. Methionine aminopeptidase type 1 subfamily. FUNCTION: Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). Plays the major role in N-terminal methionine removal. Less efficient when the second residue is Val. KEYWORDS: 3D-structure;Acetylation;Aminopeptidase;Cytoplasm;Direct protein sequencing;Hydrolase;Metal-binding;Protease;Reference proteome;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cytoplasm MISCELLANEOUS: Present with 19600 molecules/cell in log phase SD medium." Q01663,"PROTEIN NAMES: AP-1-like transcription factor (Caffeine resistance protein 3) PROTEIN FAMILY: BZIP family, YAP subfamily ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the bZIP family. YAP subfamily. FUNCTION: Transcription activator involved in multidrug resistance, oxidative stress response, and redox homeostasis. Regulates the transcription of genes encoding antioxidant enzymes like catalase ctt1 and components of the cellular thiol-reducing pathways, including the thioredoxin system (trx2, trr1), ABC transporters involved in multidrug resistance like bfr1/hba2 and pmd1 as well as the gene obr1/apt1. Preferentially binds to promoters with the core binding site 5'-TTA[CG]TAA-3'. Activity of the transcription factor is controlled through oxidation of specific cysteine residues resulting in the alteration of its subcellular location. Oxidative stress induces nuclear accumulation and as a result pap1 transcriptional activity. Required for sty1/spc1-conferred staurosporine resistance. KEYWORDS: 3D-structure;Activator;Cytoplasm;Direct protein sequencing;Disulfide bond;DNA-binding;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus Cytoplasm Note=Oxidized pap1 is found predominantly in the nucleus, while reduced pap1 is continuously exported to the cytoplasm by crm1/exportin 1. " Q01755,"PROTEIN NAMES: T-complex protein 11 (Testis-specific protein PBS13) PROTEIN FAMILY: TCP11 family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the TCP11 family. FUNCTION: Plays a role in the process of sperm capacitation and acrosome reactions. Probable receptor for the putative fertilization-promoting peptide (FPP) at the sperm membrane that may modulate the activity of the adenylyl cyclase cAMP pathway. KEYWORDS: Cell projection;Cilium;Cytoplasmic vesicle;Developmental protein;Differentiation;Flagellum;Membrane;Phosphoprotein;Reference proteome;Spermatogenesis;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Single-pass membrane protein Cell projection, cilium, flagellum Cytoplasmic vesicle, secretory vesicle, acrosome Note=Localizes on the acrosomal cap region of acrosome-intact, but not acrosome-reacted sperm. Colocalizes with MROH2B and PRKACA on the acrosome and tail regions in round spermatids and spermatozoa regardless of the capacitation status of the sperm. " Q01842,PROTEIN NAMES: Ets DNA-binding protein pokkuri (Protein anterior open) (Protein yan) PROTEIN FAMILY: ETS family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the ETS family. FUNCTION: Ets-related protein that functions as a negative regulator of photoreceptor development acting antagonistically to pnt and the proneural signal mediated by RAS. It acts upstream of SINA to inhibit R7 development. KEYWORDS: 3D-structure;Developmental protein;DNA-binding;Nucleus;Phosphoprotein;Reference proteome;Repressor;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus Note=In undifferentiated cells during the early stages of eye development. MISCELLANEOUS: 'Pokkuri' means 'dropping dead' in Japanese. Flies lacking aop result in the differentiation of supernumerary photoreceptors in the eye. Q01956,"PROTEIN NAMES: Potassium voltage-gated channel subfamily C member 3 (KSHIIID) (Voltage-gated potassium channel subunit Kv3.3) PROTEIN FAMILY: Potassium channel family, C (Shaw) subfamily, Kv3.3/KCNC3 sub-subfamily ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the potassium channel family. C (Shaw) (TC 1.A.1.2) subfamily. Kv3.3/KCNC3 sub-subfamily. FUNCTION: Voltage-gated potassium channel that plays an important role in the rapid repolarization of fast-firing brain neurons. The channel opens in response to the voltage difference across the membrane, forming a potassium-selective channel through which potassium ions pass in accordance with their electrochemical gradient. The channel displays rapid activation and inactivation kinetics. It plays a role in the regulation of the frequency, shape and duration of action potentials in Purkinje cells. Required for normal survival of cerebellar neurons, probably via its role in regulating the duration and frequency of action potentials that in turn regulate the activity of voltage-gated Ca(2+) channels and cellular Ca(2+) homeostasis. Required for normal motor function (By similarity). Plays a role in the reorganization of the cortical actin cytoskeleton and the formation of actin veil structures in neuronal growth cones via its interaction with HAX1 and the Arp2/3 complex (By similarity). KEYWORDS: Alternative splicing;Cell membrane;Cell projection;Cytoplasm;Cytoskeleton;Glycoprotein;Ion channel;Ion transport;Membrane;Methylation;Phosphoprotein;Postsynaptic cell membrane;Potassium;Potassium channel;Potassium transport;Reference proteome;Synapse;Transmembrane;Transmembrane helix;Transport;Voltage-gated channel SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Presynaptic cell membrane ; Multi-pass membrane protein Perikaryon Cell projection, axon Cell projection, dendrite Cell projection, dendritic spine membrane ; Multi-pass membrane protein Cytoplasm, cell cortex Cytoplasm, cytoskeleton Note=Detected on Purkinje cell dendritic spines, positioned perisynaptically but also in extrasynaptic positions along the spine membranes. Detected at presynaptic calices of Held (By similarity). Colocalizes with the cortical actin cytoskeleton and the Arp2/3 complex (By similarity). " Q01964,"PROTEIN NAMES: Peroxisomal biogenesis factor 2 (Peroxin-2) (Peroxisomal protein PER6) PROTEIN FAMILY: Pex2/pex10/pex12 family ORGANISM: Komagataella pastoris (Yeast) (Pichia pastoris) SIMILARITY: Belongs to the pex2/pex10/pex12 family. FUNCTION: E3 ubiquitin-protein ligase component of a retrotranslocation channel required for peroxisome organization by mediating export of the PEX5 or PEX20 receptors from peroxisomes to the cytosol, thereby promoting PEX5 and PEX20 recycling. The retrotranslocation channel is composed of PEX2, PEX10 and PEX12; each subunit contributing transmembrane segments that coassemble into an open channel that specifically allows the passage of PEX5 and PEX20 through the peroxisomal membrane (By similarity). PEX2 also regulates peroxisome organization by acting as a E3 ubiquitin-protein ligase (By similarity). PEX2 ubiquitinates PEX5 or PEX20 during their passage through the retrotranslocation channel: catalyzes monoubiquitination of PEX5 and/or PEX20 at 'Cys-6' and 'Cys-8', respectively, a modification that acts as a signal for PEX5 or PEX20 extraction into the cytosol (By similarity). PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Membrane;Metal-binding;Peroxisome;Peroxisome biogenesis;Protein transport;Transferase;Transmembrane;Transmembrane helix;Transport;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Peroxisome membrane ; Multi-pass membrane protein " Q02111,"PROTEIN NAMES: Protein kinase C theta type (nPKC-theta) PROTEIN FAMILY: Protein kinase superfamily, AGC Ser/Thr protein kinase family, PKC subfamily ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. FUNCTION: Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that mediates non-redundant functions in T-cell receptor (TCR) signaling, including T-cells activation, proliferation, differentiation and survival, by mediating activation of multiple transcription factors such as NF-kappa-B, JUN, NFATC1 and NFATC2. In TCR-CD3/CD28-co-stimulated T-cells, is required for the activation of NF-kappa-B and JUN, which in turn are essential for IL2 production, and participates in the calcium-dependent NFATC1 and NFATC2 transactivation. Mediates the activation of the canonical NF-kappa-B pathway (NFKB1) by direct phosphorylation of CARD11 on several serine residues, inducing CARD11 association with lipid rafts and recruitment of the BCL10-MALT1 complex, which then activates IKK complex, resulting in nuclear translocation and activation of NFKB1. May also play an indirect role in activation of the non-canonical NF-kappa-B (NFKB2) pathway. In the signaling pathway leading to JUN activation, acts by phosphorylating the mediator STK39/SPAK and may not act through MAP kinases signaling. Plays a critical role in TCR/CD28-induced NFATC1 and NFATC2 transactivation by participating in the regulation of reduced inositol 1,4,5-trisphosphate generation and intracellular calcium mobilization. After costimulation of T-cells through CD28 can phosphorylate CBLB and is required for the ubiquitination and subsequent degradation of CBLB, which is a prerequisite for the activation of TCR. During T-cells differentiation, plays an important role in the development of T-helper 2 (Th2) cells following immune and inflammatory responses, and, in the development of inflammatory autoimmune diseases, is necessary for the activation of IL17-producing Th17 cells. May play a minor role in Th1 response. Upon TCR stimulation, mediates T-cell protective survival signal by phosphorylating BAD, thus protecting T-cells from BAD-induced apoptosis, and by up-regulating BCL-X(L)/BCL2L1 levels through NF-kappa-B and JUN pathways. In platelets, regulates signal transduction downstream of the ITGA2B, CD36/GP4, F2R/PAR1 and F2RL3/PAR4 receptors, playing a positive role in 'outside-in' signaling and granule secretion signal transduction. May relay signals from the activated ITGA2B receptor by regulating the uncoupling of WASP and WIPF1, thereby permitting the regulation of actin filament nucleation and branching activity of the Arp2/3 complex. May mediate inhibitory effects of free fatty acids on insulin signaling by phosphorylating IRS1, which in turn blocks IRS1 tyrosine phosphorylation and downstream activation of the PI3K/AKT pathway. Phosphorylates MSN (moesin) in the presence of phosphatidylglycerol or phosphatidylinositol. Phosphorylates PDPK1 at 'Ser-504' and 'Ser-532' and negatively regulates its ability to phosphorylate PKB/AKT1. Phosphorylates CCDC88A/GIV and inhibits its guanine nucleotide exchange factor activity (By similarity). KEYWORDS: 3D-structure;ATP-binding;Cell membrane;Cytoplasm;Immunity;Inflammatory response;Kinase;Magnesium;Membrane;Metal-binding;Nucleotide-binding;Phosphoprotein;Reference proteome;Repeat;Serine/threonine-protein kinase;Transferase;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cytoplasm Cell membrane ; Peripheral membrane protein. Note=In resting T-cells, mostly localized in cytoplasm. In response to TCR stimulation, associates with lipid rafts and then localizes in the immunological synapse (By similarity). " Q02256,"PROTEIN NAMES: Tyrosine-protein phosphatase YVH1 (PTPase YVH1) PROTEIN FAMILY: Protein-tyrosine phosphatase family, Non-receptor class dual specificity subfamily ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily. FUNCTION: May be directly involved in signal transduction and/or cell cycle regulation. It is necessary for maintaining growth rate or spore germination. Could show both activity toward tyrosine-protein phosphate as well as with serine-protein phosphate. KEYWORDS: 3D-structure;Hydrolase;Phosphoprotein;Protein phosphatase;Reference proteome;Stress response MISCELLANEOUS: Present with 7570 molecules/cell in log phase SD medium." Q02337,"PROTEIN NAMES: D-beta-hydroxybutyrate dehydrogenase, mitochondrial (3-hydroxybutyrate dehydrogenase) (BDH) PROTEIN FAMILY: Short-chain dehydrogenases/reductases (SDR) family ORGANISM: Bos taurus (Bovine) SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR) family. KEYWORDS: Acetylation;Allosteric enzyme;Direct protein sequencing;Glycoprotein;Lipid metabolism;Membrane;Mitochondrion;Mitochondrion inner membrane;NAD;Oxidoreductase;Phosphoprotein;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Mitochondrion inner membrane Mitochondrion matrix " Q02354,"PROTEIN NAMES: U3 small nucleolar RNA-associated protein 6 (U3 snoRNA-associated protein 6) (U three protein 6) PROTEIN FAMILY: UTP6 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the UTP6 family. FUNCTION: Component of the SSU processome, a pre-ribosomal particle required for the maturation of the 18S rRNA from the 35S pre-rRNA precursor. KEYWORDS: 3D-structure;Nucleus;Reference proteome;Repeat;Ribonucleoprotein;Ribosome biogenesis;rRNA processing SUBCELLULAR LOCATION: Nucleus, nucleolus MISCELLANEOUS: Present with 7520 molecules/cell in log phase SD medium." Q02556,"PROTEIN NAMES: Interferon regulatory factor 8 (IRF-8) (Interferon consensus sequence-binding protein) (H-ICSBP) (ICSBP) PROTEIN FAMILY: IRF family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the IRF family. FUNCTION: Transcription factor that specifically binds to the upstream regulatory region of type I interferon (IFN) and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)). Can both act as a transcriptional activator or repressor (By similarity). Plays a negative regulatory role in cells of the immune system (By similarity). Involved in CD8(+) dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF8 and activation of genes (By similarity). Required for the development of plasmacytoid dendritic cells (pDCs), which produce most of the type I IFN in response to viral infection (By similarity). Positively regulates macroautophagy in dendritic cells. Acts as a transcriptional repressor of osteoclast differentiation factors such as NFATC1 and EEIG1 (By similarity). KEYWORDS: Activator;Autophagy;Cytoplasm;Disease variant;DNA-binding;Nucleus;Reference proteome;Repressor;Transcription;Transcription regulation;Ubl conjugation SUBCELLULAR LOCATION: Nucleus Cytoplasm Note=In resting macrophages, localizes in the cytoplasm. Translocated in the nucleus upon IFN-gamma induction. " Q02644,"PROTEIN NAMES: Growth hormone-releasing hormone receptor (GHRH receptor) (Growth hormone-releasing factor receptor) (GRF receptor) (GRFR) PROTEIN FAMILY: G-protein coupled receptor 2 family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the G-protein coupled receptor 2 family. FUNCTION: Receptor for GRF, coupled to G proteins which activate adenylyl cyclase. Stimulates somatotroph cell growth, growth hormone gene transcription and growth hormone secretion. KEYWORDS: Alternative splicing;Cell membrane;Disulfide bond;G-protein coupled receptor;Glycoprotein;Membrane;Receptor;Reference proteome;Signal;Transducer;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. " Q02725,"PROTEIN NAMES: Vacuolar transporter chaperone 3 complex subunit 3 (Phosphate metabolism protein 2) (SPX-dependent polyphosphate polymerase VTC subunit 3) (Vacuolar membrane polyphosphate polymerase accessory subunit 3) (PolyP polymerase) PROTEIN FAMILY: VTC2/3 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the VTC2/3 family. FUNCTION: Accessory subunit of the vacuolar transporter chaperone (VTC) complex. The VTC complex acts as a vacuolar polyphosphate polymerase that catalyzes the synthesis of inorganic polyphosphate (polyP) via transfer of phosphate from ATP to a growing polyP chain, releasing ADP. VTC exposes its catalytic domain VTC4 to the cytosol, where the growing polyP chain winds through a tunnel-shaped pocket, integrating cytoplasmic polymer synthesis with polyP membrane translocation. The VTC complex carries 9 vacuolar transmembrane domains, which are likely to constitute the translocation channel into the organelle lumen. PolyP synthesis is tightly coupled to its transport into the vacuole lumen, in order to avoid otherwise toxic intermediates in the cytosol, and it depends on the proton gradient across the membrane, formed by V-ATPase. The VTC complex also plays a role in vacuolar membrane fusion. Required for SEC18/NSF activity in SNARE priming, membrane binding of LMA1 and V(0) trans-complex formation. Binds inositol hexakisphosphate (Ins6P) and similar inositol polyphosphates, such as 5-diphospho-inositol pentakisphosphate (5-InsP7); these are important intracellular signaling molecules. Inositol polyphosphate binding promotes vacuolar polyphosphate synthesis. KEYWORDS: 3D-structure;Coiled coil;Cytoplasm;Cytoplasmic vesicle;Direct protein sequencing;Endoplasmic reticulum;Membrane;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix;Vacuole SUBCELLULAR LOCATION: Vacuole membrane ; Multi-pass membrane protein Cytoplasm, cell cortex Endoplasmic reticulum membrane ; Multi-pass membrane protein Cytoplasmic vesicle, autophagosome membrane ; Multi-pass membrane protein MISCELLANEOUS: Present with 2630 molecules/cell in log phase SD medium." Q02732,"PROTEIN NAMES: Inner kinetochore subunit CTF19 (CENP-P homolog) (Chromosome transmission fidelity protein 19) (Constitutive centromere-associated network protein CTF19) (Minichromosome maintenance protein 18) PROTEIN FAMILY: CENP-P/CTF19 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the CENP-P/CTF19 family. FUNCTION: Component of the kinetochore, a multiprotein complex that assembles on centromeric DNA and attaches chromosomes to spindle microtubules, mediating chromosome segregation and sister chromatid segregation during meiosis and mitosis. Component of the inner kinetochore COMA complex, which connects centromere-associated proteins and the outer kinetochore. COMA interacts with other inner kinetochore proteins to form the inner kinetochore constitutive centromere-associated network (CCAN), which serves as a structural platform for outer kinetochore assembly. KEYWORDS: 3D-structure;Cell cycle;Cell division;Centromere;Chromosome;Coiled coil;Kinetochore;Meiosis;Mitosis;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus Chromosome, centromere, kinetochore MISCELLANEOUS: Present with 1254 molecules/cell in log phase SD medium." Q02767,"PROTEIN NAMES: Vacuolar protein sorting-associated protein 28 (ESCRT-I complex subunit VPS28) PROTEIN FAMILY: VPS28 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the VPS28 family. FUNCTION: Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Required for normal endocytic and biosynthetic traffic to the yeast vacuole. KEYWORDS: 3D-structure;Cytoplasm;Endosome;Membrane;Protein transport;Reference proteome;Transport SUBCELLULAR LOCATION: Cytoplasm. Endosome. Late endosome membrane ; Peripheral membrane protein MISCELLANEOUS: Present with 1420 molecules/cell in log phase SD medium." Q02794,"PROTEIN NAMES: Protein STD1 (Glucose repression modulator MSN3) (Suppressor of Tbp deletion protein 1) (Suppressor of fluoride sensitivity 3) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: To yeast MTH1. FUNCTION: Involved in modulation of glucose-regulated gene expression. Together with MTH1, represses the hexose transporter (HXT) genes in conditions of low glucose. Stimulates the SNF1 kinase by an interaction with the catalytic domain that antagonizes autoinhibition and promotes an active conformation of the kinase. KEYWORDS: Activator;Amyloid;Cell membrane;Membrane;Nucleus;Prion;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Cell membrane ; Peripheral membrane protein Nucleus MISCELLANEOUS: The prion state [GAR+] is provoked by the interaction of the two proteins STD1 and PMA1. It involves a complex between a small fraction of the cellular complement of PMA1, and STD1, a much lower-abundance protein, and it is transmissible by non-Mendelian, cytoplasmic inheritance. [GAR+] makes cells resistant to the glucose-associated repression of alternative carbon sources. In contrast to other prion forms, [GAR+] cannot be cured by GdnHCl or by inactivation of the molecular chaperone HSP104.; MISCELLANEOUS: Present with 238 molecules/cell in log phase SD medium." Q02804,"PROTEIN NAMES: ADP-ribosylation factor-like protein 3 (Arf-like GTPase 3) PROTEIN FAMILY: Small GTPase superfamily, Arf family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the small GTPase superfamily. Arf family. FUNCTION: Involved in the targeting of ARL1 to the Golgi. Can bind and hydrolyze GTP. KEYWORDS: 3D-structure;Acetylation;Golgi apparatus;GTP-binding;Isopeptide bond;Nucleotide-binding;Protein transport;Reference proteome;Transport;Ubl conjugation SUBCELLULAR LOCATION: Golgi apparatus. MISCELLANEOUS: Present with 1920 molecules/cell in log phase SD medium." Q02890,"PROTEIN NAMES: Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase (PNGase) (Peptide:N-glycanase 1) (yPNG1) PROTEIN FAMILY: Transglutaminase-like superfamily, PNGase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the transglutaminase-like superfamily. PNGase family. FUNCTION: Specifically deglycosylates the denatured form of N-linked glycoproteins in the cytoplasm and assists their proteasome-mediated degradation. Cleaves the beta-aspartyl-glucosamine (GlcNAc) of the glycan and the amide side chain of Asn, converting Asn to Asp. Prefers proteins containing high-mannose over those bearing complex type oligosaccharides. Can recognize misfolded proteins in the endoplasmic reticulum that are exported to the cytosol to be destroyed and deglycosylate them, while it has no activity toward native proteins. Deglycosylation is a prerequisite for subsequent proteasome-mediated degradation of some, but not all, misfolded glycoproteins. Involved in the formation of free oligosaccharide in cytosol. KEYWORDS: 3D-structure;Cytoplasm;Hydrolase;Metal-binding;Nucleus;Reference proteome;Zinc SUBCELLULAR LOCATION: Cytoplasm. Nucleus. MISCELLANEOUS: Present with 4850 molecules/cell in log phase SD medium." Q02896,"PROTEIN NAMES: Alkaline ceramidase YDC1 (Acyl-CoA-independent ceramide synthase) PROTEIN FAMILY: Alkaline ceramidase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the alkaline ceramidase family. FUNCTION: Acyl-CoA-independent ceramide synthase that catalyzes the conversion of dihydroceramide and also phytoceramide to dihydrosphingosine or phytosphingosine. Prefers dihydroceramide. Very low reverse hydrolysis activity, catalyzing synthesis of dihydroceramide from fatty acid and dihydrosphingosine. Is not responsible for the breakdown of unsaturated ceramide. May play a role in heat stress response. KEYWORDS: Disulfide bond;Endoplasmic reticulum;Hydrolase;Membrane;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein " Q02928,"PROTEIN NAMES: Cytochrome P450 4A11 (20-hydroxyeicosatetraenoic acid synthase) (20-HETE synthase) (CYP4AII) (CYPIVA11) (Cytochrome P-450HK-omega) (Cytochrome P450HL-omega) (Fatty acid omega-hydroxylase) (Lauric acid omega-hydroxylase) (Long-chain fatty acid omega-monooxygenase) PROTEIN FAMILY: Cytochrome P450 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the cytochrome P450 family. FUNCTION: A cytochrome P450 monooxygenase involved in the metabolism of fatty acids and their oxygenated derivatives (oxylipins). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase). Catalyzes predominantly the oxidation of the terminal carbon (omega-oxidation) of saturated and unsaturated fatty acids, the catalytic efficiency decreasing in the following order: dodecanoic > tetradecanoic > (9Z)-octadecenoic > (9Z,12Z)-octadecadienoic > hexadecanoic acid. Acts as a major omega-hydroxylase for dodecanoic (lauric) acid in liver. Participates in omega-hydroxylation of (5Z,8Z,11Z,14Z)-eicosatetraenoic acid (arachidonate) to 20-hydroxyeicosatetraenoic acid (20-HETE), a signaling molecule acting both as vasoconstrictive and natriuretic with overall effect on arterial blood pressure. Can also catalyze the oxidation of the penultimate carbon (omega-1 oxidation) of fatty acids with lower efficiency. May contribute to the degradation of saturated very long-chain fatty acids (VLCFAs) such as docosanoic acid, by catalyzing successive omega-oxidations to the corresponding dicarboxylic acid, thereby initiating chain shortening. Omega-hydroxylates (9R,10S)-epoxy-octadecanoate stereoisomer. Plays a minor role in omega-oxidation of long-chain 3-hydroxy fatty acids. Has little activity toward prostaglandins A1 and E1. PATHWAY: Lipid metabolism; arachidonate metabolism.; PATHWAY: Lipid metabolism; oxylipin biosynthesis. KEYWORDS: Alternative splicing;Direct protein sequencing;Endoplasmic reticulum;Fatty acid metabolism;Heme;Iron;Lipid metabolism;Membrane;Metal-binding;Microsome;Monooxygenase;NADP;Oxidoreductase;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Peripheral membrane protein Microsome membrane ; Peripheral membrane protein " Q02933,"PROTEIN NAMES: Ribonuclease T2-like (RNase T2-like) PROTEIN FAMILY: RNase T2 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the RNase T2 family. FUNCTION: Rnase which modulates cell survival under stress conditions. Released from the vacuole to the cytoplasm during stress to promote tRNA and rRNA cleavage and to activate separately a downstream pathway that promotes cell death. Involved in cell size, vacuolar morphology and growth at high temperatures and high salt concentration. KEYWORDS: Cytoplasm;Disulfide bond;Endonuclease;Glycoprotein;Hydrolase;Lyase;Nuclease;Reference proteome;Signal;Vacuole SUBCELLULAR LOCATION: Vacuole lumen. Cytoplasm. Note=Is released from the vacuole to the cytoplasm during stress conditions like oxidative stress or stationary phase stress. MISCELLANEOUS: Present with 504 molecules/cell in log phase SD medium." Q02950,"PROTEIN NAMES: Small ribosomal subunit protein bS1m (37S ribosomal protein MRP51, mitochondrial) PROTEIN FAMILY: Bacterial ribosomal protein bS1 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the bacterial ribosomal protein bS1 family. FUNCTION: Component of the mitochondrial ribosome (mitoribosome), a dedicated translation machinery responsible for the synthesis of mitochondrial genome-encoded proteins, including at least some of the essential transmembrane subunits of the mitochondrial respiratory chain. The mitoribosomes are attached to the mitochondrial inner membrane and translation products are cotranslationally integrated into the membrane. bS1m functionally interacts with the 5'-UTR of mitochondrial mRNAs. KEYWORDS: 3D-structure;Mitochondrion;Phosphoprotein;Reference proteome;Ribonucleoprotein;Ribosomal protein SUBCELLULAR LOCATION: Mitochondrion Note=Mitoribosomes are tethered to the mitochondrial inner membrane and spatially aligned with the membrane insertion machinery through two distinct membrane contact sites, formed by the 21S rRNA expansion segment 96-ES1 and the inner membrane protein MBA1. MISCELLANEOUS: Present with 656 molecules/cell in log phase SD medium." Q03002,"PROTEIN NAMES: Fatty acyl-CoA synthetase and RNA processing-associated kinase 1 PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. FUNCTION: Putative serine/threonine-protein kinase that may be involved in rRNA transcription and ribosome biogenesis. KEYWORDS: ATP-binding;Cytoplasm;Kinase;Nucleotide-binding;Phosphoprotein;Reference proteome;Serine/threonine-protein kinase;Transferase SUBCELLULAR LOCATION: Cytoplasm MISCELLANEOUS: Present with 556 molecules/cell in log phase SD medium." Q03124,"PROTEIN NAMES: Chromatin structure-remodeling complex subunit RSC9 (RSC complex subunit RSC9) (Remodel the structure of chromatin complex subunit 9) PROTEIN FAMILY: RSC9 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the RSC9 family. FUNCTION: Component of the chromatin structure-remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton. This subunit plays a role in transcriptional response to stress. It is involved in both repression and activation of mRNAs regulated by the target of rapamycin (TOR) kinases, and in the synthesis of rRNA. KEYWORDS: 3D-structure;Chromatin regulator;Direct protein sequencing;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus Note=Localizes to centromeric and flanking chromatin. Association with these loci is dependent on STH1. MISCELLANEOUS: Present with 2610 molecules/cell in log phase SD medium." Q03200,"PROTEIN NAMES: Light-regulated protein, chloroplastic ORGANISM: Oryza sativa subsp. japonica (Rice) FUNCTION: Thylakoid-determinant subunit of high molecular weight LFNRs-containing protein complexes. KEYWORDS: Chloroplast;Disulfide bond;Membrane;Plastid;Reference proteome;Repeat;Thylakoid;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane ; Peripheral membrane protein ; Stromal side Plastid, chloroplast envelope Plastid, chloroplast stroma " Q03262,PROTEIN NAMES: Phosphoribomutase (PRM) (Phosphoglucomutase 3) (PGM 3) PROTEIN FAMILY: Phosphohexose mutase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the phosphohexose mutase family. FUNCTION: Major phosphoribomutase that converts ribose 1-phosphate to ribose 5-phosphate. Involved in ribose salvage via the pentose phosphate pathway. KEYWORDS: Carbohydrate metabolism;Cytoplasm;Glucose metabolism;Isomerase;Magnesium;Metal-binding;Nucleus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Nucleus MISCELLANEOUS: Present with 4960 molecules/cell in log phase SD medium. Q03337,"PROTEIN NAMES: Trafficking protein particle complex subunit 31 (TRAPP subunit 31) (Transport protein particle 31 kDa subunit) PROTEIN FAMILY: TRAPP small subunits family, BET3 subfamily ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the TRAPP small subunits family. BET3 subfamily. FUNCTION: Component of the TRAPP I, TRAPP II and TRAPP III complexes which act as guanine nucleotide exchange factors (GEF) for YPT1. TRAPP I plays a key role in the late stages of endoplasmic reticulum to Golgi traffic. TRAPP II plays a role in intra-Golgi transport. TRAPP III plays a role in autophagosome formation. KEYWORDS: 3D-structure;Autophagy;Endoplasmic reticulum;ER-Golgi transport;Golgi apparatus;Reference proteome;Transport SUBCELLULAR LOCATION: Golgi apparatus, cis-Golgi network. Endoplasmic reticulum. Preautophagosomal structure. MISCELLANEOUS: Present with 8350 molecules/cell in log phase SD medium." Q03345,"PROTEIN NAMES: Protein lin-3 (Abnormal cell lineage protein 3) (Lethal protein 94) ORGANISM: Caenorhabditis elegans FUNCTION: Probable ligand for tyrosine kinase receptor let-23. Essential for vulval induction, where it acts downstream of the synthetic multivulva (synMuv) class genes. Probably by activating let-23, phospholipase plc-3 and inositol 1,4,5-trisphosphate receptor itr-1 signaling cascade, plays a role in ovulation by promoting gonadal sheath cell contractions and spermatheca dilatation during ovulation. Probably by regulating neuronal transmission in ALA neurons, mediates the decrease in pharyngeal pumping and locomotion during the quiescent state that precedes each larval molt, by activating receptor lin-23-mediated signaling cascade.; FUNCTION: [Isoform a]: Essential for vulval induction; its activity on vulval precursor cells is partially dependent on rom-1.; FUNCTION: [Isoform c]: Essential for vulval induction; acts independently of rom-1. KEYWORDS: Alternative splicing;Disulfide bond;EGF-like domain;Glycoprotein;Growth factor;Membrane;Reference proteome;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. " Q03380,"PROTEIN NAMES: Comitin (24 kDa actin-binding protein) (CABP1-related protein p24) ORGANISM: Dictyostelium discoideum (Social amoeba) FUNCTION: May have a role in cell motility. It has high affinity for both G-actin and F-actin. Binds to vesicle membranes via mannose residues and, by way of its interaction with actin, links these membranes to the cytoskeleton. KEYWORDS: Actin-binding;Cytoplasm;Cytoskeleton;Direct protein sequencing;Golgi apparatus;Lectin;Membrane;Reference proteome;Repeat SUBCELLULAR LOCATION: Golgi apparatus membrane; Peripheral membrane protein. Endomembrane system; Peripheral membrane protein. Cytoplasm, cytoskeleton. Note=Primarily on Golgi and vesicle membranes. " Q03407,"PROTEIN NAMES: Serine/threonine-protein kinase PKH1 (3-phosphoinositide-dependent protein kinase 1) PROTEIN FAMILY: Protein kinase superfamily, AGC Ser/Thr protein kinase family, PDPK1 subfamily ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDPK1 subfamily. FUNCTION: Activates YPK1 by phosphorylating of a threonine residue. KEYWORDS: ATP-binding;Kinase;Nucleotide-binding;Phosphoprotein;Reference proteome;Serine/threonine-protein kinase;Transferase " Q03414,"PROTEIN NAMES: Myocyte-specific enhancer factor 2A homolog (XMEF2A1) (xMEF2A) (Serum response factor-like protein 2) (SL-2) PROTEIN FAMILY: MEF2 family ORGANISM: Xenopus laevis (African clawed frog) SIMILARITY: Belongs to the MEF2 family. FUNCTION: May regulate muscle-specific transcription in the embryo and may regulate transcription of a variety of cell types in the adult. Binds to the sequence 5'-CTA[TA]4TAR-3'. Acts downstream of nlk2 in anterior neural development, including eye formation. KEYWORDS: Activator;Developmental protein;DNA-binding;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus. " Q03426,"PROTEIN NAMES: Mevalonate kinase (MK) PROTEIN FAMILY: GHMP kinase family, Mevalonate kinase subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the GHMP kinase family. Mevalonate kinase subfamily. FUNCTION: Catalyzes the phosphorylation of mevalonate to mevalonate 5-phosphate, a key step in isoprenoid and cholesterol biosynthesis. PATHWAY: Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via mevalonate pathway; isopentenyl diphosphate from (R)-mevalonate: step 1/3. KEYWORDS: 3D-structure;ATP-binding;Cataract;Cholesterol biosynthesis;Cholesterol metabolism;Cytoplasm;Disease variant;Kinase;Lipid biosynthesis;Lipid metabolism;Magnesium;Metal-binding;Nucleotide-binding;Peroxisome;Reference proteome;Steroid biosynthesis;Steroid metabolism;Sterol biosynthesis;Sterol metabolism;Transferase SUBCELLULAR LOCATION: Cytoplasm Peroxisome " Q03433,"PROTEIN NAMES: Vacuolar protein sorting-associated protein 71 (SWR complex protein 6) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Participates in the catalytic exchange of histone H2A for the H2A variant HZT1, an euchromatin-specific factor, leading to chromatin remodeling and changes in transcription of targeted genes. Indirectly involved in vacuolar protein sorting. KEYWORDS: 3D-structure;Chromatin regulator;Metal-binding;Nucleus;Reference proteome;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus " Q03518,"PROTEIN NAMES: Antigen peptide transporter 1 (APT1) (ATP-binding cassette sub-family B member 2) (Peptide supply factor 1) (Peptide transporter PSF1) (PSF-1) (Peptide transporter TAP1) (Peptide transporter involved in antigen processing 1) (Really interesting new gene 4 protein) (RING4) PROTEIN FAMILY: ABC transporter superfamily, ABCB family, MHC peptide exporter subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the ABC transporter superfamily. ABCB family. MHC peptide exporter (TC 3.A.1.209) subfamily. FUNCTION: ABC transporter associated with antigen processing. In complex with TAP2 mediates unidirectional translocation of peptide antigens from cytosol to endoplasmic reticulum (ER) for loading onto MHC class I (MHCI) molecules. Uses the chemical energy of ATP to export peptides against the concentration gradient. During the transport cycle alternates between 'inward-facing' state with peptide binding site facing the cytosol to 'outward-facing' state with peptide binding site facing the ER lumen. Peptide antigen binding to ATP-loaded TAP1-TAP2 induces a switch to hydrolysis-competent 'outward-facing' conformation ready for peptide loading onto nascent MHCI molecules. Subsequently ATP hydrolysis resets the transporter to the 'inward facing' state for a new cycle. Typically transports intracellular peptide antigens of 8 to 13 amino acids that arise from cytosolic proteolysis via IFNG-induced immunoproteasome. Binds peptides with free N- and C-termini, the first three and the C-terminal residues being critical. Preferentially selects peptides having a highly hydrophobic residue at position 3 and hydrophobic or charged residues at the C-terminal anchor. Proline at position 2 has the most destabilizing effect. As a component of the peptide loading complex (PLC), acts as a molecular scaffold essential for peptide-MHCI assembly and antigen presentation. KEYWORDS: 3D-structure;Adaptive immunity;Alternative initiation;ATP-binding;Endoplasmic reticulum;Host-virus interaction;Immunity;Magnesium;Membrane;Metal-binding;Nucleotide-binding;Peptide transport;Protein transport;Reference proteome;Translocase;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein Note=The transmembrane segments seem to form a pore in the membrane. " Q03557,"PROTEIN NAMES: Glutamyl-tRNA(Gln) amidotransferase subunit A, mitochondrial (Glu-AdT subunit A) (HMG2-induced ER-remodeling protein 2) (Loss of respiratory capacity protein 6) PROTEIN FAMILY: Amidase family, GatA subfamily ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the amidase family. GatA subfamily. FUNCTION: Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln). Required for HMG2-induced ER-remodeling. KEYWORDS: 3D-structure;ATP-binding;Ligase;Mitochondrion;Nucleotide-binding;Protein biosynthesis;Reference proteome SUBCELLULAR LOCATION: Mitochondrion MISCELLANEOUS: Present with 486 molecules/cell in log phase SD medium." Q03569,"PROTEIN NAMES: G-protein regulator 2 ORGANISM: Caenorhabditis elegans FUNCTION: In the 1-cell embryo, probably together with gpr-1, controls nuclear rotation and spindle elongation during mitosis. Complex of gpr-1 and gpr-2, in association with lin-5, activates G-protein signaling to affect mitotic spindle force. Polarity determinants (par genes) may regulate lin-5/gpr-1/gpr-2/goa-1 locally to create the asymmetric forces that drive spindle movement. KEYWORDS: Cell cycle;Cell division;Cytoplasm;Cytoskeleton;Mitosis;Reference proteome SUBCELLULAR LOCATION: Cytoplasm, cell cortex Cytoplasm, cytoskeleton, spindle Note=Located to the spindle and cell cortex when in complex with lin-5 and gpr-1. During early embryogenesis, cortical localization changes with the cell cycle. In one-cell embryo, uniform cortical localization from prophase to metaphase and posterior enrichment during anaphase. In the 2-cell embryo, uniform cortical localization in AB blastomere and posterior cortical enrichment in P1 blastomere during interphase. In P1, uniform cortical localization from prophase to early anaphase and then posterior cortical enrichment during late anaphase and telophase. Cortical localization and asymmetrical distribution is regulated by the csnk-1-mediated regulation of pkk-1. Enriched at the contact site between EMS and P2 from prophase to prometaphase. " Q03611,"PROTEIN NAMES: Cyclic nucleotide-gated cation channel (Abnormal chemotaxis protein 4) PROTEIN FAMILY: Cyclic nucleotide-gated cation channel family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the cyclic nucleotide-gated cation channel (TC 1.A.1.5) family. FUNCTION: Required for normal thermosensation and chemosensation sensory behavior. Required, downstream of receptor-type guanylate cyclase gcy-9, for CO2-mediated responses in BAG neurons. Required, downstream of receptor-type guanylate cyclase gcy-14, for alkaline pH-mediated responses in ASE-left (ASEL) neurons. Involved in the development of ASJ sensory neuron axon during late larval stages and in the maintenance of normal axon morphology in the adult. Regulates dauer formation. Required for the calcium flux to the cytoplasm in the ASJ sensory neurons upon the onset and removal of a nitric oxide (NO) stimulus, thereby promoting the ASJ-mediated behavioral avoidance response to NO-producing organisms like P.aeruginosa. In ASI and ASJ sensory neurons, controls behavioral response to P.aeruginosa by up-regulating the transcription of daf-7, a member of the TGF-beta family. In AWB and AWC sensory neurons, mediates the recognition of food odors which subsequently allows for the detection of preferred food sources. In AWC neurons, acts to promote expression of srsx-3, a member of the GPCR family. KEYWORDS: 3D-structure;Cell projection;cGMP;cGMP-binding;Chemotaxis;Ion channel;Ion transport;Ligand-gated ion channel;Membrane;Neurogenesis;Nucleotide-binding;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Membrane ; Multi-pass membrane protein Cell projection, cilium " Q03649,"PROTEIN NAMES: Monoacylglycerol lipase (MAG lipase) PROTEIN FAMILY: AB hydrolase superfamily, AB hydrolase 4 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the AB hydrolase superfamily. AB hydrolase 4 family. FUNCTION: Converts monoacylglycerides (MAG) to free fatty acids and glycerol. Has a preference for palmitoyl-MAG. Does not play a significant role in ethyl ester biosynthesis. Also possesses ester hydrolase and low but persistent TAG lipase activity. KEYWORDS: Hydrolase;Isopeptide bond;Reference proteome;Serine esterase;Ubl conjugation " Q03656,"PROTEIN NAMES: Serine/threonine-protein kinase SKY1 (SRPK) PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. FUNCTION: Constitutively active kinase, specifically and sequentially phosphorylates serine/arginine (SR)-type shuttling mRNA binding proteins in their RS dipeptide repeats. KEYWORDS: 3D-structure;ATP-binding;Kinase;Nucleotide-binding;Phosphoprotein;Reference proteome;Serine/threonine-protein kinase;Transferase MISCELLANEOUS: Present with 2420 molecules/cell in log phase SD medium." Q03674,"PROTEIN NAMES: Lysophospholipase 2 (Phospholipase B 2) PROTEIN FAMILY: Lysophospholipase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the lysophospholipase family. FUNCTION: Sequentially removes both fatty acyl groups from diacylglycerophospholipids and therefore has both phospholipase A and lysophospholipase activities. However, it does not display transacylase activity. Substrate preference is phosphatidylserine > phosphatidylinositol > phosphatidylcholine > phosphatidylethanolamine. The substrate specificity is pH- and ion-dependent. In contrast with activities observed at optimum pH 3.5, the order of substrate preference at pH 5.5 is phosphatidylserine = phosphatidylethanolamine > phosphatidylcholine > phosphatidylinositol. KEYWORDS: Cell wall;Glycoprotein;GPI-anchor;Hydrolase;Lipid degradation;Lipid metabolism;Lipoprotein;Membrane;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted, cell wall. Membrane; Lipid-anchor, GPI-anchor. Note=Covalently-linked GPI-modified cell wall protein (GPI-CWP). MISCELLANEOUS: Present with 623 molecules/cell in log phase SD medium." Q03705,"PROTEIN NAMES: EKC/KEOPS complex subunit CGI121 (CGI-121 homolog) PROTEIN FAMILY: CGI121/TPRKB family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the CGI121/TPRKB family. FUNCTION: Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. CGI121 acts as an allosteric effector that regulates the t(6)A activity of the complex. The EKC/KEOPS complex also promotes both telomere uncapping and telomere elongation. The complex is required for efficient recruitment of transcriptional coactivators. CGI121 is not required for tRNA modification. KEYWORDS: 3D-structure;Activator;Chromosome;Nucleus;Reference proteome;Telomere;Transcription;Transcription regulation;tRNA processing SUBCELLULAR LOCATION: Nucleus Chromosome, telomere " Q03714,"PROTEIN NAMES: U1 SNP1-associating protein 1 ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Scaffold protein of the endoplasmic reticulum-associated degradation (ERAD) (also known as endoplasmic reticulum quality control, ERQC) pathway involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins. Component of the HRD1 ubiquitin ligase complex, which is part of the ERAD-L and ERAD-M pathways responsible for the rapid degradation of soluble lumenal and membrane proteins with misfolded lumenal domains (ERAD-L), or ER-membrane proteins with misfolded transmembrane domains (ERAD-M). Has multiple functions in ERAD including recruitment of DER1 to the HRD1 ubiquitin ligase, and regulation of HRD1 activity. Involved in oligomerization of HRD1 and in HRD1 autoubiquitination and degradation. KEYWORDS: 3D-structure;Endoplasmic reticulum;Membrane;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein MISCELLANEOUS: Present with 1890 molecules/cell in log phase SD medium." Q03723,"PROTEIN NAMES: Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST6 (Oligosaccharyl transferase subunit OST6) (Oligosaccharyl transferase 37 kDa subunit) (OTase 37 kDa subunit) PROTEIN FAMILY: OST3/OST6 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the OST3/OST6 family. FUNCTION: Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity. Can participate in redox reactions and is able to catalyze dithiol-disulfide exchange reactions with other proteins, albeit with relatively low efficiency. May form transient disulfide bonds with nascent polypeptides in the endoplasmic reticulum and thereby promote efficient glycosylation. PATHWAY: Protein modification; protein glycosylation. KEYWORDS: 3D-structure;Disulfide bond;Endoplasmic reticulum;Membrane;Reference proteome;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein MISCELLANEOUS: Present with 1080 molecules/cell in log phase SD medium." Q03751,PROTEIN NAMES: DnaJ homolog subfamily C member 5 homolog (Cysteine string protein) ORGANISM: Drosophila melanogaster (Fruit fly) FUNCTION: May have an important role in presynaptic function. KEYWORDS: Alternative splicing;Chaperone;Lipoprotein;Membrane;Palmitate;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Membrane; Lipid-anchor. Q03761,"PROTEIN NAMES: Transcription initiation factor TFIID subunit 12 (TAFII-61) (TAFII61) (TAFII-68) (TAFII68) (TBP-associated factor 12) (TBP-associated factor 61 kDa) PROTEIN FAMILY: TAF12 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the TAF12 family. FUNCTION: Functions as a component of the DNA-binding general transcription factor complex TFIID and the transcription regulatory histone acetylation (HAT) complexes SAGA, SALSA and SLIK. Binding of TFIID to a promoter (with or without TATA element) is the initial step in preinitiation complex (PIC) formation. TFIID plays a key role in the regulation of gene expression by RNA polymerase II through different activities such as transcription activator interaction, core promoter recognition and selectivity, TFIIA and TFIIB interaction, chromatin modification (histone acetylation by TAF1), facilitation of DNA opening and initiation of transcription. SAGA is involved in RNA polymerase II-dependent transcriptional regulation of approximately 10% of yeast genes. At the promoters, SAGA is required for recruitment of the basal transcription machinery. It influences RNA polymerase II transcriptional activity through different activities such as TBP interaction (SPT3, SPT8 and SPT20) and promoter selectivity, interaction with transcription activators (GCN5, ADA2, ADA3 and TRA1), and chromatin modification through histone acetylation (GCN5) and deubiquitination (UBP8). SAGA acetylates nucleosomal histone H3 to some extent (to form H3K9ac, H3K14ac, H3K18ac and H3K23ac). SAGA interacts with DNA via upstream activating sequences (UASs). SALSA, an altered form of SAGA, may be involved in positive transcriptional regulation. SLIK is proposed to have partly overlapping functions with SAGA. It preferentially acetylates methylated histone H3, at least after activation at the GAL1-10 locus. KEYWORDS: 3D-structure;Acetylation;Coiled coil;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: Present with 930 (+/-45) molecules/cell in log phase SD medium." Q03769,"PROTEIN NAMES: Epsin-5 ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Involved in the recruitment of clathrin to the Golgi network and endosomes to form clathrin coated vesicles. Plays a role in the trafficking of clathrin between the Golgi network and endosomes. Binds to membranes enriched in phosphatidylinositol-3,5-bisphosphate (PtdIns(3,5)P2) and, in association with VPS27, is involved in protein sorting at the multivesicular body (MVB). KEYWORDS: 3D-structure;Acetylation;Cytoplasm;Endosome;Lipid-binding;Membrane;Phosphoprotein;Protein transport;Reference proteome;Transport SUBCELLULAR LOCATION: Cytoplasm. Endosome membrane; Peripheral membrane protein. Note=Found predominantly on endosomal structures. MISCELLANEOUS: Present with 8100 molecules/cell in log phase SD medium." Q03771,"PROTEIN NAMES: Assembly chaperone of RPL4 PROTEIN FAMILY: ACL4 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the ACL4 family. FUNCTION: Acts as a chaperone for the L4 ribosomal subunit encoded by RPL4A and PRPL4B, required for hierarchical ribosome assembly. Shields ribosomal protein L4 until timely release and insertion into the pre-ribosome is possible, once ribosomal protein L18 is present. KEYWORDS: Acetylation;Chaperone;Cytoplasm;Nucleus;Reference proteome;Repeat;Ribosome biogenesis;TPR repeat SUBCELLULAR LOCATION: Cytoplasm Nucleus Note=Distributed throughout the cell but is enriched in the nucleus. MISCELLANEOUS: Present with 1630 molecules/cell in log phase SD medium." Q03784,"PROTEIN NAMES: Trafficking protein particle complex subunit 23 (TRAPP subunit 23) (Transport protein particle 23 kDa subunit) PROTEIN FAMILY: TRAPP small subunits family, TRAPPC4 subfamily ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the TRAPP small subunits family. TRAPPC4 subfamily. FUNCTION: Component of the TRAPP I, TRAPP II and TRAPP III complexes which act as guanine nucleotide exchange factors (GEF) for YPT1. TRAPP I plays a key role in the late stages of endoplasmic reticulum to Golgi traffic. TRAPP II plays a role in intra-Golgi transport. TRAPP III plays a role in autophagosome formation. KEYWORDS: 3D-structure;Autophagy;Endoplasmic reticulum;ER-Golgi transport;Golgi apparatus;Reference proteome;Transport SUBCELLULAR LOCATION: Golgi apparatus, cis-Golgi network. Endoplasmic reticulum. Preautophagosomal structure. MISCELLANEOUS: Present with 3130 molecules/cell in log phase SD medium." Q03785,"PROTEIN NAMES: Serine/threonine-protein kinase VHS1 (Viable in a HAL3 SIT4 background protein 1) PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. FUNCTION: Probable serine/threonine protein kinase involved in the G1-S transition. KEYWORDS: ATP-binding;Cell cycle;Cytoplasm;Kinase;Nucleotide-binding;Reference proteome;Serine/threonine-protein kinase;Transferase SUBCELLULAR LOCATION: Cytoplasm MISCELLANEOUS: Present with 780 molecules/cell in log phase SD medium." Q03790,"PROTEIN NAMES: Nucleoporin NUP53 (Nuclear pore protein NUP53) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. Active directional transport is assured by both, a Phe-Gly (FG) repeat affinity gradient for these transport factors across the NPC and a transport cofactor concentration gradient across the nuclear envelope (GSP1 and GSP2 GTPases associated predominantly with GTP in the nucleus, with GDP in the cytoplasm). NUP53 may play an important role in cell cycle regulation by inhibiting PSE1 transport functions during mitosis and sequestration of MAD1-MAD2 in a cell cycle-dependent manner. It also seems to play an important role in de novo NPC assembly by associating with nuclear membranes and driving their proliferation. KEYWORDS: 3D-structure;Acetylation;Cell cycle;Cell division;Membrane;Mitosis;mRNA transport;Nuclear pore complex;Nucleus;Phosphoprotein;Protein transport;Reference proteome;Repeat;Translocation;Transport SUBCELLULAR LOCATION: Nucleus, nuclear pore complex Nucleus membrane; Peripheral membrane protein; Cytoplasmic side. Nucleus membrane; Peripheral membrane protein; Nucleoplasmic side. Note=Symmetric distribution. MISCELLANEOUS: Present with 2060 molecules/cell in log phase SD medium." Q03835,"PROTEIN NAMES: Monothiol glutaredoxin-3 PROTEIN FAMILY: Glutaredoxin family, Monothiol subfamily ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the glutaredoxin family. Monothiol subfamily. FUNCTION: Monothiol glutaredoxin involved in the biogenesis of iron-sulfur clusters (By similarity). Binds one iron-sulfur cluster per dimer. The iron-sulfur cluster is bound between subunits, and is complexed by a bound glutathione and a cysteine residue from each subunit (Probable). KEYWORDS: 2Fe-2S;3D-structure;Iron;Iron-sulfur;Metal-binding;Redox-active center;Reference proteome MISCELLANEOUS: Present with 11000 molecules/cell in log phase SD medium." Q03862,PROTEIN NAMES: Probable metalloprotease ARX1 (Associated with ribosomal export complex protein 1) PROTEIN FAMILY: Peptidase M24 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the peptidase M24 family. FUNCTION: Probable metalloprotease involved in proper assembly of pre-ribosomal particles during the biogenesis of the 60S ribosomal subunit. Accompanies the pre-60S particles to the cytoplasm. KEYWORDS: 3D-structure;Cytoplasm;Hydrolase;Metal-binding;Metalloprotease;Nucleus;Protease;Reference proteome SUBCELLULAR LOCATION: Cytoplasm. Nucleus. MISCELLANEOUS: Present with 45100 molecules/cell in log phase SD medium. Q03941,PROTEIN NAMES: Dephospho-CoA kinase CAB5 (DPCK) (Dephosphocoenzyme A kinase) PROTEIN FAMILY: CoaE family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the CoaE family. FUNCTION: Catalyzes the phosphorylation of the 3'-hydroxyl group of dephosphocoenzyme A to form coenzyme A. PATHWAY: Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 5/5. KEYWORDS: ATP-binding;Coenzyme A biosynthesis;Endoplasmic reticulum;Kinase;Mitochondrion;Nucleotide-binding;Nucleus;Reference proteome;Transferase SUBCELLULAR LOCATION: Endoplasmic reticulum Mitochondrion Nucleus Note=Nuclear envelope. MISCELLANEOUS: Present with 1660 molecules/cell in log phase SD medium. Q03957,"PROTEIN NAMES: CTD kinase subunit alpha (CTDK-I subunit alpha) (CTD kinase 58 kDa subunit) (CTD kinase subunit 1) PROTEIN FAMILY: Protein kinase superfamily, CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. FUNCTION: Catalytic subunit of the CTDK-I complex, which hyperphosphorylates the C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit. CTDK-I phosphorylates 'Ser-5' if the CTD substrate is not phosphorylated at 'Ser-5', but will phosphorylate 'Ser-2' of a CTD substrate if 'Ser-5' is already phosphorylated. CTDK-I is also more reactive toward substrates that are prephosphorylated at 'Ser-2' or 'Ser-5' compared with an unphosphorylated CTD substrate, therefore efficiently creating doubly phosphorylated CTD repeats. Involved in RNA polymerase II transcriptional elongation, and through PTI1, pre-mRNA 3'-end processing. Participates in both positive and negative regulation of CTD phosphorylation. Required for DNA damage induced transcription, including the expression of the RNR genes, and reprogramming of gene expression upon amino acid starvation. Required for SET2 mediated H3K36 methylation. Also regulates H3K4 methylation. Controls the maintenance of suppressive chromatin in the coding regions of genes by both promoting H3K36 methylation, which leads to histone deacetylation, and catalyzing phosphorylation of the CTD required to localize H3K4 chromatin modification specifically to the 5' ends of genes, thereby creating a boundary for H3K4 methylation that prevents a mark associated with transcriptional initiation from spreading into the bodies of genes. Involved in RNA polymerase I transcription. Involved in telomere maintenance. Acts together with SNF1 to induce GSY2 transcription in response to glucose limitation. Involved in the adaptation to alternative carbon sources, including galactose, glycerol and ethanol, but not raffinose. Required for the integrity of the rDNA locus. Functions in translation elongation by enhancing decoding fidelity. Needed for translational accuracy by phosphorylating RPS2. KEYWORDS: 3D-structure;ATP-binding;Cytoplasm;DNA damage;Kinase;mRNA processing;Nucleotide-binding;Nucleus;Phosphoprotein;Protein biosynthesis;Reference proteome;Serine/threonine-protein kinase;Stress response;Transcription;Transferase SUBCELLULAR LOCATION: Nucleus, nucleolus. Cytoplasm. MISCELLANEOUS: Present with 125 molecules/cell in log phase SD medium." Q03973,"PROTEIN NAMES: High mobility group protein 1 (High spontaneous mutagenesis protein 2) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: DNA-binding protein that is probably part of the rDNA transcription apparatus. Acts synergetically with the RPA49 subunit of RNA polymerase I during rDNA transcription. May participate in mutagenesis control. KEYWORDS: Direct protein sequencing;DNA-binding;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus, nucleolus Note=Colocalizes with FOB1. MISCELLANEOUS: Present with 19000 molecules/cell in log phase SD medium." Q04003,"PROTEIN NAMES: Something about silencing protein 4 ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Component of the SAS complex, a multiprotein complex that acetylates 'Lys-16' of histone H4 and 'Lys-14' of histone H3. The SAS complex is however unable to acetylate nucleosomal histones. The complex is involved in transcriptional silencing at telomeres and at HML locus. Also involved in rDNA silencing. In the complex, SAS4 is essential for histone acetyltransferase (HAT) activity of the complex. KEYWORDS: Chromatin regulator;Coiled coil;Nucleus;Reference proteome;Repressor;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: Present with 800 molecules/cell in log phase SD medium." Q04047,PROTEIN NAMES: Protein no-on-transient A (Puff-specific protein Bj6) ORGANISM: Drosophila melanogaster (Fruit fly) FUNCTION: Required for normal vision and courtship behavior in Drosophila. KEYWORDS: Alternative splicing;Coiled coil;Phosphoprotein;Reference proteome;Repeat;RNA-binding;Sensory transduction;Vision Q04048,"PROTEIN NAMES: Pre-mRNA-splicing factor SYF1 (PRP19-associated complex protein 90) (Synthetic lethal with CDC40 protein 1) PROTEIN FAMILY: Crooked-neck family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the crooked-neck family. FUNCTION: Involved in pre-mRNA splicing and cell cycle control. As a component of the NTC complex (or PRP19-associated complex), associates to the spliceosome to mediate conformational rearrangement or to stabilize the structure of the spliceosome after U4 snRNA dissociation, which leads to spliceosome maturation. KEYWORDS: 3D-structure;Direct protein sequencing;mRNA processing;mRNA splicing;Nucleus;Reference proteome;Repeat;Spliceosome SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: Present with 2170 molecules/cell in log phase SD medium." Q04080,PROTEIN NAMES: GPI transamidase component GPI17 PROTEIN FAMILY: PIGS family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the PIGS family. FUNCTION: Component of the GPI transamidase complex. Involved in transfer of GPI to proteins. PATHWAY: Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis. KEYWORDS: Endoplasmic reticulum;Glycoprotein;GPI-anchor biosynthesis;Membrane;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein MISCELLANEOUS: Present with 7520 molecules/cell in log phase SD medium. Q04089,"PROTEIN NAMES: Histone-lysine N-methyltransferase, H3 lysine-79 specific (Disrupter of telomere silencing protein 1) (Histone H3-K79 methyltransferase) (H3-K79-HMTase) (Lysine N-methyltransferase 4) PROTEIN FAMILY: Class I-like SAM-binding methyltransferase superfamily, DOT1 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase superfamily. DOT1 family. FUNCTION: Histone methyltransferase that specifically trimethylates histone H3 to form H3K79me3. This methylation is required for telomere silencing and for the pachytene checkpoint during the meiotic cell cycle by allowing the recruitment of RAD9 to double strand breaks. Nucleosomes are preferred as substrate compared to free histones. Can bind to DNA (in vitro). KEYWORDS: 3D-structure;Chromatin regulator;DNA-binding;Methyltransferase;Nucleus;Reference proteome;Repeat;S-adenosyl-L-methionine;Transcription;Transcription regulation;Transferase SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: In contrast to other lysine histone methyltransferases, it does not contain a SET domain, suggesting the existence of another mechanism for methylation of lysine residues of histones.; MISCELLANEOUS: Present with 2160 molecules/cell in log phase SD medium." Q04149,"PROTEIN NAMES: Crossover junction endonuclease MUS81 (MMS and UV-sensitive protein 81) PROTEIN FAMILY: XPF family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the XPF family. FUNCTION: Interacts with MMS4 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, D-loops, replication forks with regressed leading strands and nicked Holliday junctions. Cleavage probably occurs approximately half a helical turn upstream of the free 5'-end in these structures. May be required in mitosis for the processing of stalled replication fork intermediates arising spontaneously or subsequent to treatment with DNA damaging agents such as methylmethane sulfonate (MMS), camptothecin (CPT) or UV. May be required in meiosis for the repair of meiosis-specific double strand breaks subsequent to single-end invasion (SEI). This involves consecutive cleavage of D-loops and nicked Holliday junctions leading to sister chromatid crossover. In contrast to MSH4-MSH5 dependent crossover, double Holliday junctions do not seem to be involved. Spore formation and viability are severely impaired in deletion strains. KEYWORDS: DNA damage;DNA recombination;DNA repair;Endonuclease;Hydrolase;Magnesium;Manganese;Meiosis;Metal-binding;Nuclease;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: Two distinct classes of meiotic crossovers have been demonstrated in budding yeast. Class I crossovers exhibit crossover interference and require MSH4 and MSH5 for their resolution, while class II crossovers exhibit no crossover interference and require MUS81 and MMS4. While class I crossovers represent the majority of crossovers in S.cerevisiae, they are virtually absent in S.pombe, which lacks orthologs of MSH4 and MSH5.; MISCELLANEOUS: Present with 300 molecules/cell in log phase SD medium." Q04175,"PROTEIN NAMES: Importin beta SMX1 (Suppressor of mRNA export mutant protein 1) (karyopherin-108) PROTEIN FAMILY: Importin beta family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the importin beta family. FUNCTION: Nuclear transport factor (karyopherin) involved in protein transport between the cytoplasm and nucleoplasm. Required for the nuclear import of ribosomal proteins (RPL11, RPL16, RPL25, RPL31A), the poly(A)-binding protein PAB1, the HO endonuclease or the tRNA and snRNA chaperone LHP1. Indirectly involved in nuclear mRNA export through its PAB1 nuclear import activity. KEYWORDS: Cytoplasm;mRNA transport;Nuclear pore complex;Nucleus;Protein transport;Reference proteome;Translocation;Transport SUBCELLULAR LOCATION: Cytoplasm. Nucleus, nuclear pore complex. MISCELLANEOUS: Present with 16300 molecules/cell in log phase SD medium." Q04178,"PROTEIN NAMES: Hypoxanthine-guanine phosphoribosyltransferase (HGPRT) (HGPRTase) (Bypass of repression by adenine protein 6) PROTEIN FAMILY: Purine/pyrimidine phosphoribosyltransferase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the purine/pyrimidine phosphoribosyltransferase family. FUNCTION: Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway. PATHWAY: Purine metabolism; IMP biosynthesis via salvage pathway; IMP from hypoxanthine: step 1/1. KEYWORDS: 3D-structure;Acetylation;Cytoplasm;Glycosyltransferase;Magnesium;Metal-binding;Nucleotide-binding;Nucleus;Purine salvage;Reference proteome;Transferase SUBCELLULAR LOCATION: Cytoplasm Nucleus MISCELLANEOUS: Present with 36500 molecules/cell in log phase SD medium." Q04195,PROTEIN NAMES: E3 SUMO-protein ligase SIZ1 (E3 SUMO-protein transferase SIZ2) (SAP and Miz-finger domain-containing protein 1) (Ubiquitin-like protein ligase 1) PROTEIN FAMILY: PIAS family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the PIAS family. FUNCTION: Acts as an E3 ligase mediating SUMO/Smt3 attachment to septins and PCNA. May be involved in chromosome maintenance. PATHWAY: Protein modification; protein sumoylation. KEYWORDS: 3D-structure;Cytoplasm;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Transferase;Ubl conjugation;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cytoplasm Nucleus Bud neck Note=Present at the bud neck in early M-phase. MISCELLANEOUS: Present with 149 molecules/cell in log phase SD medium. Q04226,"PROTEIN NAMES: Transcription initiation factor TFIID subunit 11 (TAFII-40) (TAFII40) (TBP-associated factor 11) (TBP-associated factor 40 kDa) (P40) PROTEIN FAMILY: TAF11 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the TAF11 family. FUNCTION: Functions as a component of the DNA-binding general transcription factor complex TFIID. Binding of TFIID to a promoter (with or without TATA element) is the initial step in pre-initiation complex (PIC) formation. TFIID plays a key role in the regulation of gene expression by RNA polymerase II through different activities such as transcription activator interaction, core promoter recognition and selectivity, TFIIA and TFIIB interaction, chromatin modification (histone acetylation by TAF1), facilitation of DNA opening and initiation of transcription. KEYWORDS: Direct protein sequencing;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus. " Q04305,"PROTEIN NAMES: U3 small nucleolar RNA-associated protein 15 (U3 snoRNA-associated protein 15) (U three protein 15) (U3 protein 15 required for transcription) (t-UTP15) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Involved in nucleolar processing of pre-18S ribosomal RNA. Required for optimal pre-ribosomal RNA transcription by RNA polymerase I together with a subset of U3 proteins required for transcription (t-UTPs). KEYWORDS: 3D-structure;Nucleus;Reference proteome;Repeat;Ribonucleoprotein;Ribosome biogenesis;rRNA processing;Transcription;WD repeat SUBCELLULAR LOCATION: Nucleus, nucleolus Note=Associated with ribosomal chromatin, even in the absence of transcription. MISCELLANEOUS: Present with 358 molecules/cell in log phase SD medium." Q04359,PROTEIN NAMES: Sporulation-specific protein 20 PROTEIN FAMILY: SNAP-25 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the SNAP-25 family. FUNCTION: Required to maintain the prospore membrane to the nucleus during sporulation in order to capture the daughter nuclei and form the spores. Mediates the fusion of exocytic vesicles with the plasma membrane during sporulation through its interactions with the t-SNARE SSO1 and v-SNARE SNC2. KEYWORDS: Cell membrane;Coiled coil;Membrane;Reference proteome;Sporulation SUBCELLULAR LOCATION: Cell membrane. Prospore membrane. Note=Membrane-associated to the plasma during vegetative growth and prospore membrane associated during sporulation. Q04364,"PROTEIN NAMES: Peroxisomal targeting signal receptor 9 (PTS1 receptor 9) (PTS1R 9) (Peroxin-9) PROTEIN FAMILY: Peroxisomal targeting signal receptor family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the peroxisomal targeting signal receptor family. FUNCTION: Peroxisomal import receptor that mediates the peroxisomal import of both malate synthases MLS1 and MLS2 in oleate-grown cells. Recognizes the C-terminal peroxisomal targeting signal PTS1 sequence SKL of MLS1 and MLS2, probably via its TPR domains. Interacts with the PTS1-receptor docking protein PEX14 but not with peroxins PEX1, PEX3 through to PEX8, PEX10, PEX11, PEX12, PEX13, PEX15, PEX17, PEX18, PEX19 and PEX21. KEYWORDS: Cytoplasm;Membrane;Peroxisome;Reference proteome;Repeat;TPR repeat SUBCELLULAR LOCATION: Cytoplasm Peroxisome membrane ; Peripheral membrane protein " Q04370,"PROTEIN NAMES: Peroxisome assembly protein 12 (Peroxin-12) PROTEIN FAMILY: Pex2/pex10/pex12 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the pex2/pex10/pex12 family. FUNCTION: Component of a retrotranslocation channel required for peroxisome organization by mediating export of the PEX5 receptor from peroxisomes to the cytosol, thereby promoting PEX5 recycling. The retrotranslocation channel is composed of PEX2, PEX10 and PEX12; each subunit contributing transmembrane segments that coassemble into an open channel that specifically allows the passage of PEX5 through the peroxisomal membrane. PEX12 also regulates PEX5 recycling by activating the E3 ubiquitin-protein ligase activity of PEX10. When PEX5 recycling is compromised, PEX12 stimulates PEX10-mediated polyubiquitination of PEX5, leading to its subsequent degradation. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: 3D-structure;Membrane;Metal-binding;Peroxisome;Protein transport;Reference proteome;Transmembrane;Transmembrane helix;Transport;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Peroxisome membrane ; Multi-pass membrane protein MISCELLANEOUS: Present with 907 molecules/cell in log phase SD medium." Q04371,"PROTEIN NAMES: Damage-control phosphatase YMR027W (Sugar phosphate phosphatase YMR027W) PROTEIN FAMILY: Damage-control phosphatase family, Sugar phosphate phosphatase III subfamily ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the damage-control phosphatase family. Sugar phosphate phosphatase III subfamily. FUNCTION: Metal-dependent phosphatase that shows phosphatase activity against several substrates, including fructose-1-phosphate and fructose-6-phosphate. Its preference for fructose-1-phosphate, a strong glycating agent that causes DNA damage rather than a canonical yeast metabolite, suggests a damage-control function in hexose phosphate metabolism. KEYWORDS: 3D-structure;Hydrolase;Manganese;Metal-binding;Nickel;Reference proteome MISCELLANEOUS: Present with 6190 molecules/cell in log phase SD medium." Q04377,"PROTEIN NAMES: DNA damage checkpoint protein LCD1 (DNA damage checkpoint protein 2) (Lethal, checkpoint-defective, DNA damage-sensitive protein 1) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Forms a complex with the serine/threonine kinase MEC1 which activates checkpoint signaling upon genotoxic stresses. The MEC1-LCD1 complex is recruited by the single-strand-binding protein complex RPA to DNA lesions in order to initiate the DNA repair by homologous recombination, after the MRX-complex and TEL1 are displaced. Required for the recruitment of MEC1 to DNA lesions, the activation of CHK1 and RAD53 kinases and phosphorylation of RAD9 in response to DNA damage. Required for cell growth and meiotic recombination. KEYWORDS: 3D-structure;Chromatin regulator;Coiled coil;Cytoplasm;DNA damage;DNA repair;Nucleus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Localizes to nuclear DNA repair foci with other DNA repair proteins in response to DNA double strand breaks. The recruitment to DNA lesion sites requires the presence of the RPA complex on DNA. MISCELLANEOUS: Present with 606 molecules/cell in log phase SD medium." Q04410,"PROTEIN NAMES: GRASP65 homolog protein 1 ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Involved in the spindle assembly checkpoint. Involved in ER to Golgi vesicle-mediated transport by either facilitating USO1-dependent and -independent tethering or increasing target accuracy of fusion events of COPII-coated vesicles. KEYWORDS: 3D-structure;Acetylation;Cytoplasm;ER-Golgi transport;Golgi apparatus;Membrane;Phosphoprotein;Reference proteome;Repeat;Transport SUBCELLULAR LOCATION: Cytoplasm. Golgi apparatus, cis-Golgi network membrane; Peripheral membrane protein. Note=Localizes to cytoplasm in a punctate pattern. Association with the cis-Golgi requires N-terminal acetylation and is probably mediated via an N-terminal amphipathic helix. The localization to the Golgi is MAK3-dependent and SYS1-independent. Localizes to the Golgi together with BUG1. MISCELLANEOUS: Present with 2730 molecules/cell in log phase SD medium." Q04458,"PROTEIN NAMES: Fatty aldehyde dehydrogenase HFD1 (Hexadecenal dehydrogenase) PROTEIN FAMILY: Aldehyde dehydrogenase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the aldehyde dehydrogenase family. FUNCTION: Catalyzes the oxidation of long-chain aliphatic aldehydes to fatty acids. Responsible for conversion of the sphingosine 1-phosphate (S1P) degradation product hexadecenal to hexadecenoic acid. Involved in coenzyme Q (CoQ) biosynthesis, catalyzing the last step in the tyrosine to 4-hydroxybenzoate (4-HB) pathway. Oxidizes 4-hydroxybenzaldehyde (4-Hbz) to 4-HB, the aromatic precursor for coenzyme Q. KEYWORDS: Endosome;Lipid droplet;Membrane;Mitochondrion;Mitochondrion outer membrane;NAD;Oxidoreductase;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Lipid droplet Mitochondrion outer membrane ; Single-pass membrane protein Endosome membrane ; Single-pass membrane protein Cytoplasmic granule membrane ; Single-pass membrane protein MISCELLANEOUS: Present with 2930 molecules/cell in log phase SD medium." Q04493,PROTEIN NAMES: Prefoldin subunit 5 (Genes involved in microtubule biogenesis protein 5) (Gim complex subunit 5) (GimC subunit 5) PROTEIN FAMILY: Prefoldin subunit alpha family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the prefoldin subunit alpha family. FUNCTION: Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins. KEYWORDS: Chaperone;Reference proteome MISCELLANEOUS: Present with 2900 molecules/cell in log phase SD medium. Q04508,"PROTEIN NAMES: Ammonia monooxygenase beta subunit (AMO) (Heterotrimeric Cu-heme enzyme) ORGANISM: Nitrosomonas europaea (strain ATCC 19718 / CIP 103999 / KCTC 2705 / NBRC 14298) FUNCTION: Part of the ammonia monooxygenase complex, which catalyzes the oxidation of ammonia to hydroxylamine, the first reaction in the process of ammonia oxidation to nitrite. KEYWORDS: Cell membrane;Copper;Cytoplasm;Direct protein sequencing;Membrane;Metal-binding;Monooxygenase;Oxidoreductase;Reference proteome;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Cytoplasm Note=Ammonia monooxygenase is active and distributed approximately equally in both subcellular fractions. MISCELLANEOUS: The physiological significance of the two gene copies is still unknown." Q04638,"PROTEIN NAMES: E3 ubiquitin-protein ligase ITT1 PROTEIN FAMILY: RBR family, RNF14 subfamily ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the RBR family. RNF14 subfamily. FUNCTION: E3 ubiquitin-protein ligase involved in translation quality control. Involved in the rescue of stalled ribosomes by promoting ubiquitination and degradation of proteins on stalled ribosomes. Specifically required to resolve RNA-protein cross-links caused by reactive aldehydes, which trigger translation stress by stalling ribosomes: acts by catalying 'Lys-6'-linked ubiquitination of RNA-protein cross-links, leading to their degradation. Interacts with the translation termination factors eRF1 (SUP45) and eRF3 (SUP35); overexpression decreases the efficiency of translation termination. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Metal-binding;Reference proteome;Repeat;Transferase;Translation regulation;Ubl conjugation pathway;Zinc;Zinc-finger MISCELLANEOUS: Present with 846 molecules/cell in log phase SD medium." Q04651,"PROTEIN NAMES: ER-derived vesicles protein ERV41 PROTEIN FAMILY: ERGIC family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the ERGIC family. FUNCTION: Constituent of COPII-coated endoplasmic reticulum-derived transport vesicles. Required for efficient transport of a subset of secretory proteins to the Golgi. The C-terminal Ile-Leu motif is required for exit from the endoplasmic reticulum. Facilitates retrograde transport from the Golgi to the endoplasmic reticulum. KEYWORDS: 3D-structure;Cytoplasmic vesicle;Direct protein sequencing;Endoplasmic reticulum;ER-Golgi transport;Golgi apparatus;Membrane;Protein transport;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein Golgi apparatus membrane ; Multi-pass membrane protein. Cytoplasmic vesicle, COPII-coated vesicle membrane ; Multi-pass membrane protein Note=Recycles between endoplasmic reticulum and Golgi. Resides in the endoplasmic and Golgi compartments, and then packaged into endoplasmic reticulum derived vesicles. MISCELLANEOUS: Present with 3000 molecules/cell in log phase SD medium." Q04659,PROTEIN NAMES: Chromosome segregation in meiosis protein 3 PROTEIN FAMILY: CSM3 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the CSM3 family. FUNCTION: Forms a fork protection complex (FPC) with TOF1 which is required for chromosome segregation during meiosis and DNA damage repair. FPC coordinates leading and lagging strand synthesis and moves with the replication fork. FPC stabilizes replication forks in a configuration that is recognized by replication checkpoint sensors and protects stalled replication forks against the fork-releasing activity of RRM3 helicase. KEYWORDS: 3D-structure;Cell cycle;DNA damage;DNA repair;DNA replication inhibitor;Meiosis;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: Present with 358 molecules/cell in log phase SD medium. Q04665,"PROTEIN NAMES: Guanine nucleotide-binding protein alpha-2 subunit (GP2-alpha) PROTEIN FAMILY: G-alpha family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the G-alpha family. FUNCTION: Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. This protein may be involved in the determination of the cAMP level according to nutritional conditions, most probably as a regulator of adenylyl cyclase. KEYWORDS: GTP-binding;Magnesium;Metal-binding;Nucleotide-binding;Reference proteome;Transducer " Q04673,"PROTEIN NAMES: General transcription and DNA repair factor IIH subunit SSL1 (TFIIH subunit SSL1) (RNA polymerase II transcription factor B subunit SSL1) (TFB subunit SSL1) (Suppressor of stem-loop protein 1) PROTEIN FAMILY: GTF2H2 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the GTF2H2 family. FUNCTION: Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to TFIIK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module TFIIK controls the initiation of transcription. KEYWORDS: 3D-structure;DNA damage;DNA repair;Metal-binding;Nucleus;Reference proteome;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: Present with 2340 molecules/cell in log phase SD medium." Q04688,PROTEIN NAMES: DNA-binding protein Ets97D (D-elg) PROTEIN FAMILY: ETS family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the ETS family. FUNCTION: May have a role in germline development. KEYWORDS: DNA-binding;Nucleus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Nucleus. Q04739,"PROTEIN NAMES: SNF1 protein kinase subunit beta-3 (Glucose repression protein GAL83) (Protein SPM1) PROTEIN FAMILY: 5'-AMP-activated protein kinase beta subunit family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the 5'-AMP-activated protein kinase beta subunit family. FUNCTION: Beta subunit of the SNF1 kinase complex, which is required for transcriptional, metabolic, and developmental adaptations in response to glucose limitation. Has a structural role, mediating heterotrimer formation, and a regulatory role, defining carbon source-regulated subcellular location and substrate specificity of the SNF1 kinase complex. Promotes the relocalization of the SNF1 kinase complex to the nucleus upon shift to nonfermentable carbon sources. KEYWORDS: Cytoplasm;Nucleus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Nucleus Note=Resides in the cytosol during growth on fermentable carbon sources and relocalizes rapidly to the nucleus in response to carbon stress. MISCELLANEOUS: Present with 3590 molecules/cell in log phase SD medium." Q04790,"PROTEIN NAMES: Interferon alpha/beta receptor 1 (IFN-R-1) (IFN-alpha/beta receptor 1) (Type I interferon receptor 1) PROTEIN FAMILY: Type II cytokine receptor family ORGANISM: Bos taurus (Bovine) SIMILARITY: Belongs to the type II cytokine receptor family. FUNCTION: Together with IFNAR2, forms the heterodimeric receptor for type I interferons (including interferons alpha, beta, epsilon, omega and kappa). Type I interferon binding activates the JAK-STAT signaling cascade, resulting in transcriptional activation or repression of interferon-regulated genes that encode the effectors of the interferon response (By similarity). Mechanistically, type I interferon-binding brings the IFNAR1 and IFNAR2 subunits into close proximity with one another, driving their associated Janus kinases (JAKs) (TYK2 bound to IFNAR1 and JAK1 bound to IFNAR2) to cross-phosphorylate one another (By similarity). The activated kinases phosphorylate specific tyrosine residues on the intracellular domains of IFNAR1 and IFNAR2, forming docking sites for the STAT transcription factors (By similarity). STAT proteins are then phosphorylated by the JAKs, promoting their translocation into the nucleus to regulate expression of interferon-regulated genes (By similarity). Can also act independently of IFNAR2: form an active IFNB1 receptor by itself and activate a signaling cascade that does not involve activation of the JAK-STAT pathway (By similarity). KEYWORDS: Cell membrane;Disulfide bond;Endosome;Glycoprotein;Isopeptide bond;Lipoprotein;Lysosome;Membrane;Palmitate;Phosphoprotein;Receptor;Reference proteome;Repeat;Signal;Transmembrane;Transmembrane helix;Ubl conjugation SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein Late endosome Lysosome Note=Interferon binding triggers internalization of the receptor from the cell membrane into endosomes and then into lysosomes. " Q04841,"PROTEIN NAMES: DNA-3-methyladenine glycosylase (3-alkyladenine DNA glycosylase) (3-methyladenine DNA glycosidase) (ADPG) (N-methylpurine-DNA glycosylase) PROTEIN FAMILY: DNA glycosylase MPG family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the DNA glycosylase MPG family. FUNCTION: Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, and 7-methylguanine from the damaged DNA polymer formed by alkylation lesions. KEYWORDS: Cytoplasm;DNA damage;DNA repair;Hydrolase;Mitochondrion;Mitochondrion nucleoid;Nucleus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Mitochondrion matrix, mitochondrion nucleoid Nucleus " Q04921,"PROTEIN NAMES: Sporulation-regulated protein 28 PROTEIN FAMILY: TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily, Septin GTPase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family. FUNCTION: Septins are GTPases involved in cytokinesis that assemble into filaments and form a ring at the cleavage site. May act by recruiting MYO1 and HOF1, a protein involved in septation, to the site of cleavage. Septins are also involved in cell morphogenesis, bud site selection, chitin deposition, cell cycle regulation, cell compartmentalization and spore wall formation (By similarity). KEYWORDS: Cell cycle;Cell division;Coiled coil;GTP-binding;Membrane;Nucleotide-binding;Reference proteome SUBCELLULAR LOCATION: Membrane ; Peripheral membrane protein Bud neck Note=Present at the bud neck during cell division. Probably interacts with phosphoinosides such as phosphatidylinositol 4-phosphate or phosphatidylinositol 5-phosphate (By similarity). Localizes to ring-like structures around each of the four nuclear lobes at the onset and during meiosis II. Concentrated initially at the leading edge of the developing prospore wall. " Q04924,"PROTEIN NAMES: Glucosidase 2 subunit beta (Alpha-glucosidase 2 subunit beta) (Alpha-glucosidase II subunit beta) (Glucosidase II subunit beta) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Subunit of glucosidase 2, which cleaves sequentially the 2 innermost alpha-1,3-linked glucose residues from the Glc(2)Man(9)GlcNAc(2) oligosaccharide precursor of immature glycoproteins. Specifically required for the cleavage of the final glucose. KEYWORDS: Coiled coil;Disulfide bond;Endoplasmic reticulum;Glycoprotein;Reference proteome;Signal SUBCELLULAR LOCATION: Endoplasmic reticulum MISCELLANEOUS: Present with 9760 molecules/cell in log phase SD medium." Q04929,"PROTEIN NAMES: Adapter molecule crk (Proto-oncogene c-Crk) (p38) PROTEIN FAMILY: CRK family ORGANISM: Gallus gallus (Chicken) SIMILARITY: Belongs to the CRK family. FUNCTION: May mediate attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility (By similarity). Involved in cell branching and adhesion (By similarity). May regulate the EFNA5-EPHA3 signaling (By similarity). KEYWORDS: 3D-structure;Cell membrane;Cytoplasm;Membrane;Phosphoprotein;Proto-oncogene;Reference proteome;Repeat;SH2 domain;SH3 domain SUBCELLULAR LOCATION: Cytoplasm Cell membrane Note=Translocated to the plasma membrane upon cell adhesion. " Q04941,PROTEIN NAMES: Proteolipid protein 2 (Differentiation-dependent protein A4) (Intestinal membrane A4 protein) ORGANISM: Homo sapiens (Human) FUNCTION: May play a role in cell differentiation in the intestinal epithelium. KEYWORDS: Alternative splicing;Glycoprotein;Membrane;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. Q04951,"PROTEIN NAMES: Probable family 17 glucosidase SCW10 (Soluble cell wall protein 10) PROTEIN FAMILY: Glycosyl hydrolase 17 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the glycosyl hydrolase 17 family. FUNCTION: Glucanases possibly play a role in cell expansion during growth, in cell-cell fusion during mating, and in spore release during sporulation. KEYWORDS: Cell wall;Cell wall biogenesis/degradation;Cleavage on pair of basic residues;Direct protein sequencing;Glycoprotein;Glycosidase;Hydrolase;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted, cell wall MISCELLANEOUS: Present with 10500 molecules/cell in log phase SD medium." Q04HB7,"PROTEIN NAMES: Lysine racemase PROTEIN FAMILY: Alanine racemase family ORGANISM: Oenococcus oeni (strain ATCC BAA-331 / PSU-1) SIMILARITY: Belongs to the alanine racemase family. FUNCTION: Catalyzes the interconversion of D-lysine and L-lysine. Can also use arginine and ornithine, but not alanine. KEYWORDS: 3D-structure;Isomerase;Pyridoxal phosphate;Reference proteome " Q04J43,"PROTEIN NAMES: Serine/threonine-protein kinase StkP (Ser/Thr-protein kinase StkP) (Eukaryotic-type Ser/Thr protein kinase) (ESTPK) PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family ORGANISM: Streptococcus pneumoniae serotype 2 (strain D39 / NCTC 7466) SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. FUNCTION: Protein kinase involved in signal transduction pathways that regulate various cellular processes. Likely senses intracellular peptidoglycan subunits present in the cell division septa of actively growing cells; thus, intracellular unlinked peptidoglycan may serve as the signal molecules that trigger StkP phosphorylation activity on a set of substrates. Plays a crucial role in the regulation of cell shape and cell division of S.pneumoniae through control of at least DivIVA activity. Is involved in competence triggering, and is required for the expression of the central competence operon comCDE. StkP also plays an important role for bacterial survival in vivo. Identified target substrates that are specifically phosphorylated by StkP in vivo, mainly on threonine residues, are DivIVA, GlmM, PpaC, MapZ, KhpB (also called EloR/Jag, shown in strains R6 and Rx1) and StkP itself. Autophosphorylated StkP is a substrate for the cotranscribed protein phosphatase PhpP (shown in the avirulent strain Rx / Cp1015); PhpP and StkP appear to constitute a functional signaling couple in vivo. KEYWORDS: ATP-binding;Cell cycle;Cell division;Cell membrane;Cell shape;Competence;Kinase;Membrane;Nucleotide-binding;Phosphoprotein;Reference proteome;Repeat;Septation;Serine/threonine-protein kinase;Transferase;Transmembrane;Transmembrane helix;Virulence SUBCELLULAR LOCATION: Cell membrane ; Single-pass membrane protein Note=Localizes to the midcell division sites, colocalizes with PBP2b. MISCELLANEOUS: previous research shows that the patterns of phosphorylated proteins are similar for S.pneumoniae Rx / Cp1015 and D39 (avirulent and virulent strains, respectively) when they are grown in complex CAT medium. Thus, in both strains, StkP phosphorylates the same set of substrates that are probably important for sustaining normal growth in laboratory conditions." Q04LE4,"PROTEIN NAMES: Cell division protein FtsX PROTEIN FAMILY: ABC-4 integral membrane protein family, FtsX subfamily ORGANISM: Streptococcus pneumoniae serotype 2 (strain D39 / NCTC 7466) SIMILARITY: Belongs to the ABC-4 integral membrane protein family. FtsX subfamily. FUNCTION: Part of the ABC transporter FtsEX involved in asymmetric cellular division facilitating the initiation of sporulation (By similarity). Required in maintaining normal growth and cellular morphology. KEYWORDS: 3D-structure;Cell cycle;Cell division;Cell membrane;Membrane;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein " A6QP84,"PROTEIN NAMES: Sodium-dependent organic anion transporter (Soat) (Solute carrier family 10 member 6) (SLC10A6) PROTEIN FAMILY: Bile acid:sodium symporter (BASS) family ORGANISM: Bos taurus (Bovine) SIMILARITY: Belongs to the bile acid:sodium symporter (BASS) (TC 2.A.28) family. FUNCTION: Transports sulfoconjugated steroid hormones from the extracellular compartment into the cytosol in a sodium-dependent manner without hydrolysis. Steroid sulfate hormones are commonly considered to be biologically inactive metabolites, that may be activated by steroid sulfatases into free steroids (By similarity). May play an important role by delivering sulfoconjugated steroids to specific target cells in reproductive organs (By similarity). May play a role transporting the estriol precursor 16alpha-hydroxydehydroepiandrosterone 3-sulfate (16a-OH-DHEAS) at the fetal blood vessel endothelium (By similarity). Can also transport other sulfoconjugated molecules such as taurolithocholic acid-3-sulfate and sulfoconjugated pyrenes (By similarity). KEYWORDS: Alternative splicing;Glycoprotein;Ion transport;Lipid transport;Membrane;Reference proteome;Sodium;Sodium transport;Symport;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Membrane ; Multi-pass membrane protein " A6XA80,"PROTEIN NAMES: Leukotriene C4 synthase (LTC4 synthase) (Glutathione S-transferase LTC4) (Leukotriene-C(4) synthase) PROTEIN FAMILY: MAPEG family ORGANISM: Cavia porcellus (Guinea pig) SIMILARITY: Belongs to the MAPEG family. FUNCTION: Catalyzes the conjugation of leukotriene A4 with reduced glutathione (GSH) to form leukotriene C4 with high specificity. Can also catalyze the transfer of a glutathionyl group from glutathione (GSH) to 13(S),14(S)-epoxy-docosahexaenoic acid to form maresin conjugate in tissue regeneration 1 (MCTR1), a bioactive lipid mediator that possess potent anti-inflammatory and proresolving actions. PATHWAY: Lipid metabolism; leukotriene C4 biosynthesis. KEYWORDS: Endoplasmic reticulum;Leukotriene biosynthesis;Lyase;Membrane;Nucleus;Phosphoprotein;Reference proteome;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Nucleus outer membrane ; Multi-pass membrane protein Endoplasmic reticulum membrane ; Multi-pass membrane protein Nucleus membrane ; Multi-pass membrane protein " A6ZXH8,"PROTEIN NAMES: Autophagy-related protein 9 (Cytoplasm to vacuole targeting protein 7) PROTEIN FAMILY: ATG9 family ORGANISM: Saccharomyces cerevisiae (strain YJM789) (Baker's yeast) SIMILARITY: Belongs to the ATG9 family. FUNCTION: Phospholipid scramblase involved in autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation. Cycles between the preautophagosomal structure/phagophore assembly site (PAS) and the cytoplasmic vesicle pool and supplies membrane for the growing autophagosome. Lipid scramblase activity plays a key role in preautophagosomal structure/phagophore assembly by distributing the phospholipids that arrive through ATG2 from the cytoplasmic to the luminal leaflet of the bilayer, thereby driving autophagosomal membrane expansion. Required for mitophagy. Also involved in endoplasmic reticulum-specific autophagic process and is essential for the survival of cells subjected to severe ER stress. Different machineries are required for anterograde trafficking to the PAS during either the Cvt pathway or bulk autophagy and for retrograde trafficking. Recruits vesicle-tethering proteins TRS85 and YPT1 to the autophagosome formation site. Recruits also ATG23 and ATG8 to the PAS. KEYWORDS: Autophagy;Cytoplasmic vesicle;Endoplasmic reticulum;Golgi apparatus;Isopeptide bond;Lipid transport;Membrane;Mitochondrion;Phosphoprotein;Transmembrane;Transmembrane helix;Transport;Ubl conjugation SUBCELLULAR LOCATION: Preautophagosomal structure membrane ; Multi-pass membrane protein Cytoplasmic vesicle membrane ; Multi-pass membrane protein Golgi apparatus membrane ; Multi-pass membrane protein Endoplasmic reticulum membrane ; Multi-pass membrane protein Mitochondrion membrane ; Multi-pass membrane protein Note=The vast majority of ATG9 exists on cytoplasmic mobile vesicles (designated ATG9 vesicles) that were derived from the Golgi apparatus in a process involving ATG23 and ATG27. The peripheral pool of ATG9 partially colocalizes within tubulovesicular clusters adjacent to mitochondria. It appears that membrane that contains ATG9 is delivered to the autophagosome from the Golgi-endosomal system rather than from the ER or mitochondria. Within the PAS, localizes at the edge of the isolation membrane. ATG9 cycling is regulated by at least the conserved oligomeric Golgi (COG) complex, ARP2, HOG1, PIK1, SEC2, SEC9, SSO1 and SSO2. " A7DTF0,"PROTEIN NAMES: Mortality factor related protein 1 ORGANISM: Caenorhabditis elegans FUNCTION: Protein involved in the remodeling of chromatin thereby regulating various processes including transcription, chromosome synapsis and genome integrity. Mainly binds genomic loci carrying trimethylated histone H3 'Lys-36' (H3K36me3) or 'Lys-4' (H3K4me3), and acetylated histone H3 'Lys-9' (H3K9ac), 'Lys-27' (H3K27ac). During meiosis, required for the presynaptic pairing of homologous chromosomal regions outside of the pairing center and for the progression of chromosome synapsis. Essential maternal factor required in postembryonic germline development and in maintaining germ cell identity. Plays an important role in maintaining genomic integrity in primordial germ cells (PGCs) during meiosis by regulating DNA double-strand break (DSB) repair and synapsis. Also, required for chromatin-based transcriptional silencing in PGCs and for silencing of X-linked genes in the maternal germ line. By retaining histone acetyltransferase, cbp-1, in euchromatin, promotes the anchoring of heterochromatin at the inner nuclear membrane in intestinal and hypodermal cells. KEYWORDS: Alternative splicing;Chromatin regulator;Chromosome;DNA damage;DNA repair;Meiosis;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus Chromosome Note=Concentrated on euchromatic regions marked by H3K36me2/me3 and probably enriched on autosomes. " A7E3N2,"PROTEIN NAMES: Neutrophil cytosol factor 2 (NCF-2) (Neutrophil NADPH oxidase factor 2) PROTEIN FAMILY: NCF2/NOXA1 family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the NCF2/NOXA1 family. FUNCTION: NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production). KEYWORDS: Cytoplasm;Phosphoprotein;Reference proteome;Repeat;SH3 domain;TPR repeat SUBCELLULAR LOCATION: Cytoplasm " A7HD43,"PROTEIN NAMES: Globin-coupled histidine kinase (AfGcHK) (Heme-based oxygen-sensor histidine kinase) ORGANISM: Anaeromyxobacter sp. (strain Fw109-5) FUNCTION: Member of the two-component regulatory system GcHK/Anae109_2439. Autophosphorylates in response to oxygen availability, and then transfers the phosphate group to a conserved Asp residue in the receiver domains of the cognate response regulator Anae109_2439, resulting in its activation. KEYWORDS: 3D-structure;ATP-binding;Heme;Iron;Kinase;Metal-binding;Nucleotide-binding;Phosphoprotein;Reference proteome;Transferase;Two-component regulatory system " A7MCY6,PROTEIN NAMES: TANK-binding kinase 1-binding protein 1 (TBK1-binding protein 1) ORGANISM: Homo sapiens (Human) FUNCTION: Adapter protein which constitutively binds TBK1 and IKBKE playing a role in antiviral innate immunity. KEYWORDS: Alternative splicing;Coiled coil;Host-virus interaction;Immunity;Innate immunity;Metal-binding;Phosphoprotein;Reference proteome;Zinc;Zinc-finger A7WM73,"PROTEIN NAMES: Beta-hexosaminidase 1 (Beta-GlcNAcase 1) (Beta-N-acetylhexosaminidase 1) (Beta-hexosaminidase 2) (AtHEX2) (N-acetyl-beta-glucosaminidase 1) PROTEIN FAMILY: Glycosyl hydrolase 20 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the glycosyl hydrolase 20 family. FUNCTION: Has a broad substrate specificity. Can use synthetic substrates such as pyridylaminated chitotriose, pyridylaminated chitobiose, p-nitrophenyl-beta-N-acetylglucosaminide, p-nitrophenyl-2-acetamido-2-deoxy-beta-D-glucopyranoside (pNP-GlcNAc), p-nitrophenyl-2-acetamido-2-deoxy-beta-D-galactopyranoside (pNP-GalNAc), 4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside (MU-GlcNAc), and 4-methylumbelliferyl-6-sulfo-2-acetamido-2-deoxy-beta-D-glucopyranoside (MU-GlcNAc-6SO(4)) as substrates. Removes terminal GlcNAc residues from alpha1,3- and alpha1,6-mannosyl branches of biantennary N-glycans without any strict branch preference. Required for the presence of paucimannosidic N-glycans in glycoproteins of roots and, to a lower extent, of leaves. KEYWORDS: Disulfide bond;Glycoprotein;Glycosidase;Hydrolase;Reference proteome;Signal;Vacuole SUBCELLULAR LOCATION: Vacuole " A7XDQ9,"PROTEIN NAMES: Hydroxyproline O-galactosyltransferase GALT2 (AtGALT2) (Beta-1,3-galactosyltransferase 20) PROTEIN FAMILY: Glycosyltransferase 31 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the glycosyltransferase 31 family. FUNCTION: Possesses hydroxyproline O-galactosyltransferase activity. Transfers galactose from UDP-galactose to hydroxyproline residues in the arabinogalactan proteins (AGPs). Is specific for AGPs containing non-contiguous peptidyl hydroxyproline residues. Utilizes UDP-galactose solely as sugar donor. The addition of galactose onto the peptidyl hydroxyproline residues in AGP core proteins represents the first committed step in arabinogalactan polysaccharide addition. AGP glycans play essential roles in both vegetative and reproductive plant growth. PATHWAY: Protein modification; protein glycosylation. KEYWORDS: Alternative splicing;Glycoprotein;Glycosyltransferase;Golgi apparatus;Manganese;Membrane;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus membrane ; Single-pass type II membrane protein " A7XGN8,"PROTEIN NAMES: Disease susceptibility protein LOV1 (Disease resistance protein RPP8-like protein 1) (Protein LONG VEGETATIVE PHASE1) PROTEIN FAMILY: Disease resistance NB-LRR family, RPP8/HRT subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the disease resistance NB-LRR family. RPP8/HRT subfamily. FUNCTION: Confers susceptibility to the fungus Cochliobolus victoriae by conditioning victorin-dependent (victorin is a toxin synthesized by C.victoriae) induction of defense-associated proteins. KEYWORDS: ATP-binding;Coiled coil;Leucine-rich repeat;Nucleotide-binding;Plant defense;Repeat " A8DYY6,"PROTEIN NAMES: Schwannomin-interacting protein 1 homolog PROTEIN FAMILY: SCHIP1 family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the SCHIP1 family. FUNCTION: Regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein Hippo (hpo), in complex with its regulatory protein Salvador (sav), phosphorylates and activates Warts (wts) in complex with its regulatory protein Mats, which in turn phosphorylates and inactivates the Yorkie (yki) oncoprotein. Schip1 promotes kinase activity of Tao and enhances phosphorylation of hpo by Tao. KEYWORDS: Cell junction;Cell membrane;Coiled coil;Membrane;Reference proteome SUBCELLULAR LOCATION: Cell junction, adherens junction Apical cell membrane ; Peripheral membrane protein " A8E5V9,"PROTEIN NAMES: Stimulator of interferon genes protein (STING) (Transmembrane protein 173) PROTEIN FAMILY: STING family ORGANISM: Xenopus tropicalis (Western clawed frog) (Silurana tropicalis) SIMILARITY: Belongs to the STING family. FUNCTION: Sensor of cytosolic DNA from bacteria and viruses that promotes autophagy. Acts by recognizing and binding cyclic GMP-AMP (cGAMP), a messenger produced by CGAS in response to DNA in the cytosol. Exhibits guanine base-specific ligand recognition: binds 3'-3'linked cGAMP, 2'-3' linked cGAMP and 3'-3' linked c-di-GMP with much greater affinity as compared to 3'-3' linked c-di-AMP. Following cGAMP-binding, promotes the formation of autophagosomes, leading to target cytosolic DNA for degradation by the lysosome. Promotes autophagy by acting as a proton channel that directs proton efflux from the Golgi to facilitate LC3 lipidation (By similarity). Lacks the C-terminal tail (CTT) found in other vertebrate orthologs which is essential for interferon signaling. KEYWORDS: Autophagy;Cytoplasm;Cytoplasmic vesicle;Endoplasmic reticulum;Golgi apparatus;Immunity;Innate immunity;Ion channel;Ion transport;Membrane;Nucleotide-binding;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein Cytoplasm, perinuclear region Endoplasmic reticulum-Golgi intermediate compartment membrane ; Multi-pass membrane protein Golgi apparatus membrane ; Multi-pass membrane protein Cytoplasmic vesicle, autophagosome membrane ; Multi-pass membrane protein Note=In response to double-stranded DNA stimulation, translocates from the endoplasmic reticulum through the endoplasmic reticulum-Golgi intermediate compartment and Golgi to post-Golgi vesicles, where the kinase tbk1 is recruited. Upon cGAMP-binding, translocates to the endoplasmic reticulum-Golgi intermediate compartment (ERGIC) in a process that is dependent on COPII vesicles; STING1-containing ERGIC serves as a membrane source for LC3 lipidation, which is a key step in autophagosome biogenesis (By similarity). " A8FNH9,"PROTEIN NAMES: Carboxynorspermidine/carboxyspermidine decarboxylase (CANS DC/CAS DC) (CANSDC/CASDC) PROTEIN FAMILY: Orn/Lys/Arg decarboxylase class-II family, NspC subfamily ORGANISM: Campylobacter jejuni subsp. jejuni serotype O:6 (strain 81116 / NCTC 11828) SIMILARITY: Belongs to the Orn/Lys/Arg decarboxylase class-II family. NspC subfamily. FUNCTION: Catalyzes the decarboxylation of carboxynorspermidine and carboxyspermidine in vitro. In vivo, responsible for synthesizing spermidine, but not sym-norspermidine. KEYWORDS: 3D-structure;Cytoplasm;Decarboxylase;Lyase;Polyamine biosynthesis;Pyridoxal phosphate;Spermidine biosynthesis SUBCELLULAR LOCATION: Cytoplasm " A8MR93,"PROTEIN NAMES: Dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase (Alpha-1,6-mannosyltransferase ALG12) (Asparagine-linked glycosylation protein 12) (EMS-mutagenized BRI1 suppressor 4) PROTEIN FAMILY: Glycosyltransferase 22 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the glycosyltransferase 22 family. FUNCTION: Mannosyltransferase that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. In the lumen of the endoplasmic reticulum, adds the eighth mannose residue in an alpha-1,6 linkage onto Man(7)GlcNAc(2)-PP-dolichol to produce Man(8)GlcNAc(2)-PP-dolichol. PATHWAY: Protein modification; protein glycosylation. KEYWORDS: Alternative splicing;Endoplasmic reticulum;Glycoprotein;Glycosyltransferase;Membrane;Reference proteome;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein MISCELLANEOUS: In the absence of ALG12 activity, the N-glycans transferred to proteins are aberrant, indicating that the oligosaccharyltransferase (OST) complex is substrate-tolerant." A8MS41,"PROTEIN NAMES: Carbon catabolite repressor protein 4 homolog 4 (CCR4 homolog 4) (Protein HESPERIN) (AtHESP) (AtHesperin) PROTEIN FAMILY: CCR4/nocturin family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the CCR4/nocturin family. FUNCTION: Acts as a catalytic component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover (By similarity). Transcriptional regulator of circadian rhythms with poly(A)-degrading activity that affects the expression and rhythmicity of the clock core oscillator genes TOC1 and CCA1. Deadenylation may be a mechanism involved in the regulation of the circadian clock. May play a negative role in response against oxidative stress. Possesses magnesium-dependent poly(A)-specific exoribonuclease activity in vitro and is almost inactive with poly(U), poly(C) and poly(G) as substrates. KEYWORDS: Acetylation;Alternative splicing;Biological rhythms;Cytoplasm;Exonuclease;Hydrolase;Magnesium;Metal-binding;Nuclease;Nucleus;Reference proteome;Repeat;RNA-binding;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus Cytoplasm MISCELLANEOUS: Plants overexpressing CCR4-4 exhibit retarded growth phenotype and severe reduction in root length that is attenuated over time.; MISCELLANEOUS: [Isoform 4]: May be due to intron retention.; MISCELLANEOUS: [Isoform 5]: May be due to intron retention." A8MS68,"PROTEIN NAMES: Dihydrolipoyl dehydrogenase 1, chloroplastic (ptLPD1) (Dihydrolipoamide dehydrogenase 1) (Protein LIPOAMIDE DEHYDROGENASE 1) (Pyruvate dehydrogenase complex E3 subunit 1) (E3-1) (PDC-E3 1) PROTEIN FAMILY: Class-I pyridine nucleotide-disulfide oxidoreductase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family. FUNCTION: Lipoamide dehydrogenase is a component of the plastidial pyruvate dehydrogenase complex (PDC). KEYWORDS: Alternative splicing;Chloroplast;Disulfide bond;FAD;Flavoprotein;NAD;Oxidoreductase;Plastid;Redox-active center;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast stroma MISCELLANEOUS: The active site is a redox-active disulfide bond." A8WFJ9,"PROTEIN NAMES: Transcription factor ets-4 PROTEIN FAMILY: ETS family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the ETS family. FUNCTION: Transcription factor which binds to 5'-GGAA/T-3' DNA consensus sequences. Both positively and negatively regulates the expression of target genes. Plays a role in the regulation of adult lifespan, which may in part be through modulation of daf-16 activity. Regulates the expression of genes such as svh-2 in response to axon injury and in addition, may function downstream of the cAMP signaling pathway to promote axon regeneration. Regulates the expression of lipid metabolism genes and may also control the expression of the RNA-binding protein rege-1 which too has been implicated in the control of fat accumulation. KEYWORDS: Alternative splicing;DNA-binding;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " A8X811,"PROTEIN NAMES: Armadillo repeat-containing protein wrm-1 (Worm armadillo protein 1) ORGANISM: Caenorhabditis briggsae FUNCTION: Antagonistic role in the Wnt signaling pathway that operates in embryogenesis (By similarity). When located at the cortex it has been shown to inhibit Wnt signaling during asymmetric cell division but when relocated to the nucleus it shows positive regulation (By similarity). Has a role in blastomere signaling during endoderm specification (By similarity). Component of the beta-catenin-lit-1 complex which promotes phosphorylation, down-regulation and subcellular relocation of pop-1 (By similarity). Within the complex, activates lit-1-dependent kinase activity (By similarity). Can substitute for bar-1 indicating functional redundancy (By similarity). Appears to have a role in centrosome positioning (By similarity). Involved in the development of distal tip cells (DTC) by regulating the asymmetric distribution of cye-1 and cki-1 between the daughters of Z1.a and Z4.p cells (By similarity). KEYWORDS: Activator;Cytoplasm;Developmental protein;Nucleus;Reference proteome;Transcription;Transcription regulation;Wnt signaling pathway SUBCELLULAR LOCATION: Cytoplasm, cell cortex Nucleus Note=Located in the anterior cell cortex before and during asymmetric cell division. After division, located preferentially in the nucleus of the posterior daughter cell (By similarity). " A8YPR6,"PROTEIN NAMES: Snake venom metalloprotease inhibitor 02D01 (02E11) (10F07) (Svmpi-Eoc7) [Cleaved into: Tripeptide pEKW 1; Tripeptide pEKW 2; Tripeptide pEKW 3; Tripeptide pEKW 4; Tripeptide pEKW 5; Tripeptide pEKW 6; Tripeptide pEKW 7; Tripeptide pEKW 8; Tripeptide pEKW 9; Tripeptide pEKW 10; Tripeptide pEKW 11; Poly-His-poly-Gly peptide 4 (pHpG-4); Poly-His-poly-Gly peptide 3 (pHpG-3); Poly-His-poly-Gly peptide 2 (pHpG-2); Poly-His-poly-Gly peptide 1 (pHpG-1); C-type natriuretic peptide (CNP)] PROTEIN FAMILY: Natriuretic peptide family; PHpG family ORGANISM: Echis ocellatus (Ocellated saw-scaled viper) SIMILARITY: In the C-terminal section; belongs to the natriuretic peptide family.; SIMILARITY: In the central section; belongs to the pHpG family. FUNCTION: pEKW and poly-His-poly-Gly peptides may serve as metalloproteinase inhibitors during glandular storage. Their inhibition may be instantly disengaged, by dilution or physiochemical change, when venom is injected into tissue of the prey.; FUNCTION: [C-type natriuretic peptide]: has a vasorelaxant activity in rat aortic strips and a diuretic potency in anesthetized rats (By similarity). May act by activating natriuretic receptors (NPR1 and/or NPR2). KEYWORDS: Cleavage on pair of basic residues;Direct protein sequencing;Disulfide bond;Hypotensive agent;Metalloenzyme inhibitor;Metalloprotease inhibitor;Protease inhibitor;Pyrrolidone carboxylic acid;Repeat;Secreted;Signal;Toxin;Vasoactive;Vasodilator SUBCELLULAR LOCATION: Secreted " A9CB27,"PROTEIN NAMES: Zinc finger protein ZPR1 (Zinc finger protein 259) PROTEIN FAMILY: ZPR1 family ORGANISM: Papio anubis (Olive baboon) SIMILARITY: Belongs to the ZPR1 family. FUNCTION: Acts as a signaling molecule that communicates proliferative growth signals from the cytoplasm to the nucleus. Plays a role for the localization and accumulation of the survival motor neuron protein SMN1 in sub-nuclear bodies, including gems and Cajal bodies. Induces neuron differentiation and stimulates axonal growth and formation of growth cone in spinal cord motor neurons. Plays a role in the splicing of cellular pre-mRNAs. May be involved in H(2)O(2)-induced neuronal cell death (By similarity). KEYWORDS: Cell projection;Cytoplasm;Differentiation;Metal-binding;mRNA processing;mRNA splicing;Nucleus;Reference proteome;Repeat;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Cytoplasm Nucleus, nucleolus Cytoplasm, perinuclear region Nucleus, gem Nucleus, Cajal body Cell projection, axon Cell projection, growth cone Note=Localized predominantly in the cytoplasm in serum-starved cells growth arrested in G0 of the mitotic cell cycle. Localized both in the nucleus and cytoplasm at the G1 phase of the mitotic cell cycle. Accumulates in the subnuclear bodies during progression into the S phase of the mitotic cell cycle. Diffusely localized throughout the cell during mitosis. Colocalized with NPAT and SMN1 in nuclear bodies including gems (Gemini of coiled bodies) and Cajal bodies in a cell cycle-dependent manner. Colocalized with EGFR in the cytoplasm of quiescent cells. Translocates from the cytoplasm to the nucleus in a epidermal growth factor (EGF)-dependent manner. Translocates together with EEF1A1 from the cytoplasm to the nucleolus after treatment with mitogens. Colocalized with SMN1 in Gemini of coiled bodies (gems), Cajal bodies, axon and growth cones of neurons (By similarity). " A9CK16,PROTEIN NAMES: tRNA-specific adenosine deaminase PROTEIN FAMILY: Cytidine and deoxycytidylate deaminase family ORGANISM: Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens (strain C58)) SIMILARITY: Belongs to the cytidine and deoxycytidylate deaminase family. FUNCTION: Catalyzes the deamination of adenosine to inosine at the wobble position 34 of tRNA(Arg2). KEYWORDS: 3D-structure;Hydrolase;Metal-binding;Reference proteome;tRNA processing;Zinc A9JTS5,"PROTEIN NAMES: Speckle targeted PIP5K1A-regulated poly(A) polymerase (Star-PAP) (RNA-binding motif protein 21) (RNA-binding protein 21) (U6 snRNA-specific terminal uridylyltransferase 1) (U6-TUTase) PROTEIN FAMILY: DNA polymerase type-B-like family ORGANISM: Xenopus tropicalis (Western clawed frog) (Silurana tropicalis) SIMILARITY: Belongs to the DNA polymerase type-B-like family. FUNCTION: Poly(A) polymerase that creates the 3'-poly(A) tail of specific pre-mRNAs. In addition to polyadenylation, it is also required for the 3'-end cleavage of pre-mRNAs: binds to the 3'UTR of targeted pre-mRNAs and promotes the recruitment and assembly of the CPSF complex on the 3'UTR of pre-mRNAs. In addition to adenylyltransferase activity, also has uridylyltransferase activity. However, the ATP ratio is higher than UTP in cells, suggesting that it functions primarily as a poly(A) polymerase. KEYWORDS: ATP-binding;Magnesium;Manganese;Metal-binding;mRNA processing;Nucleotide-binding;Nucleotidyltransferase;Nucleus;Reference proteome;RNA-binding;Transferase;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus, nucleolus Nucleus speckle " A9QT41,"PROTEIN NAMES: NF-kappa-B essential modulator (NEMO) (FIP-3) (IkB kinase-associated protein 1) (IKKAP1) (Inhibitor of nuclear factor kappa-B kinase subunit gamma) (I-kappa-B kinase subunit gamma) (IKK-gamma) (IKKG) (IkB kinase subunit gamma) (NF-kappa-B essential modifier) ORGANISM: Sus scrofa (Pig) FUNCTION: Regulatory subunit of the IKK core complex which phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Its binding to scaffolding polyubiquitin plays a key role in IKK activation by multiple signaling receptor pathways. Can recognize and bind both 'Lys-63'-linked and linear polyubiquitin upon cell stimulation, with a much highr affinity for linear polyubiquitin. Could be implicated in NF-kappa-B-mediated protection from cytokine toxicity. Essential for viral activation of IRF3. Involved in TLR3- and IFIH1-mediated antiviral innate response; this function requires 'Lys-27'-linked polyubiquitination. KEYWORDS: Coiled coil;Cytoplasm;Disulfide bond;DNA damage;Isopeptide bond;Kinase;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation;Transferase;Ubl conjugation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cytoplasm Nucleus Note=Sumoylated NEMO accumulates in the nucleus in response to genotoxic stress. " A9ULC7,"PROTEIN NAMES: Organic solute transporter subunit alpha (OST-alpha) (Solute carrier family 51 subunit alpha) PROTEIN FAMILY: OST-alpha family ORGANISM: Xenopus tropicalis (Western clawed frog) (Silurana tropicalis) SIMILARITY: Belongs to the OST-alpha family. FUNCTION: Essential component of the Ost-alpha/Ost-beta complex, a heterodimer that acts as the intestinal basolateral transporter responsible for the translocation of bile acids (such as taurocholate), steroids (such as estrone sulfate), and eicosanoids (such as prostaglandin E2). KEYWORDS: Cell membrane;Endoplasmic reticulum;Glycoprotein;Lipid transport;Membrane;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Endoplasmic reticulum membrane ; Multi-pass membrane protein " A9ULR9,"PROTEIN NAMES: BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 1 (BTB/POZ domain-containing protein KCTD13) PROTEIN FAMILY: BACURD family ORGANISM: Danio rerio (Zebrafish) (Brachydanio rerio) SIMILARITY: Belongs to the BACURD family. FUNCTION: Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex required for synaptic transmission (By similarity). The BCR(KCTD13) E3 ubiquitin ligase complex mediates the ubiquitination of RHOA, leading to its degradation by the proteasome, thereby regulating the actin cytoskeleton and promoting synaptic transmission. KEYWORDS: Nucleus;Reference proteome;Ubl conjugation pathway SUBCELLULAR LOCATION: Nucleus " A9ZPH9,"PROTEIN NAMES: Glutamate--methylamine ligase (Gamma-glutamylmethylamide synthetase) (GMAS) (Glutamate--ethylamine ligase) (N(5)-ethyl-L-glutamine synthetase) (Theanine synthetase) PROTEIN FAMILY: Glutamine synthetase family, Type 3 subfamily ORGANISM: Methylovorus mays SIMILARITY: Belongs to the glutamine synthetase family. Type 3 subfamily. FUNCTION: Catalyzes the formation of N(5)-methyl-L-glutamine from glutamate and methylamine. In vitro, can also use ethylamine, hydroxylamine and ammonia, with 75%, 40% and 1% activity compared to methylamine, respectively. KEYWORDS: ATP-binding;Direct protein sequencing;Ligase;Magnesium;Nucleotide-binding " B0F481,"PROTEIN NAMES: Bifunctional dethiobiotin synthetase/7,8-diamino-pelargonic acid aminotransferase, mitochondrial (Bifunctional BIO3-BIO1 protein) [Includes: Dethiobiotin synthetase (DTB synthetase) (DTBS) (Protein BIOTIN AUXOTROPH 3); 7,8-diamino-pelargonic acid aminotransferase (DAPA AT) (DAPA aminotransferase) (7,8-diaminononanoate synthase) (DANS) (Adenosylmethionine-8-amino-7-oxononanoate aminotransferase) (Diaminopelargonic acid synthase) (Protein BIOTIN AUXOTROPH 1)] PROTEIN FAMILY: Dethiobiotin synthetase family; Class-III pyridoxal-phosphate-dependent aminotransferase family, BioA subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: In the N-terminal section; belongs to the dethiobiotin synthetase family.; SIMILARITY: In the C-terminal section; belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. BioA subfamily. FUNCTION: Bifunctional enzyme that catalyzes two different reactions involved in the biotin biosynthesis.; FUNCTION: Catalyzes a mechanistically unusual reaction, the ATP-dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8-diaminopelargonic acid (DAPA) to form an ureido ring.; FUNCTION: Catalyzes the transfer of the alpha-amino group from S-adenosyl-L-methionine (SAM) to 7-keto-8-aminopelargonic acid (KAPA) to form 7,8-diaminopelargonic acid (DAPA). It is the only aminotransferase known to utilize SAM as an amino donor. PATHWAY: Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2.; PATHWAY: Cofactor biosynthesis; biotin biosynthesis; 7,8-diaminononanoate from 8-amino-7-oxononanoate (SAM route): step 1/1. KEYWORDS: 3D-structure;Alternative splicing;Aminotransferase;ATP-binding;Biotin biosynthesis;Ligase;Magnesium;Metal-binding;Mitochondrion;Multifunctional enzyme;Nucleotide-binding;Pyridoxal phosphate;Reference proteome;S-adenosyl-L-methionine;Transferase;Transit peptide SUBCELLULAR LOCATION: Mitochondrion matrix MISCELLANEOUS: [Isoform 2]: May be due to a competing acceptor splice site.; MISCELLANEOUS: [Isoform 3]: May be due to a competing acceptor splice site.; MISCELLANEOUS: [Isoform 4]: May be due to an intron retention." B0F9L4,"PROTEIN NAMES: Golgin candidate 6 (AtGC6) (Protein MAIGO 4) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Golgi matrix protein playing a role in tethering of vesicles to Golgi membranes and in maintaining the overall structure of the Golgi apparatus. Functions in the anterograde transport of storage protein precursors from the endoplasmic reticulum (ER) to the Golgi complex. KEYWORDS: Coiled coil;Golgi apparatus;Phosphoprotein;Protein transport;Reference proteome;Transport SUBCELLULAR LOCATION: Golgi apparatus Golgi apparatus, Golgi stack Note=Concentrates only on one side of the Golgi bodies. " B0G0Y8,"PROTEIN NAMES: cGMP-specific 3',5'-cGMP phosphodiesterase 3 (Phosphodiesterase 3) (DdPDE3) PROTEIN FAMILY: Cyclic nucleotide phosphodiesterase family ORGANISM: Dictyostelium discoideum (Social amoeba) SIMILARITY: Belongs to the cyclic nucleotide phosphodiesterase family. FUNCTION: Phosphodiesterase specific for cGMP, which is not activated by cGMP. Involved in the degradation of intracellular cGMP. KEYWORDS: cGMP;cGMP-binding;Cytoplasm;Hydrolase;Manganese;Metal-binding;Nucleotide-binding;Reference proteome SUBCELLULAR LOCATION: Cytoplasm, cytosol " B0JZV4,"PROTEIN NAMES: Cytosolic carboxypeptidase-like protein 5 (ATP/GTP-binding protein-like 5) (Protein deglutamylase CCP5) PROTEIN FAMILY: Peptidase M14 family ORGANISM: Xenopus tropicalis (Western clawed frog) (Silurana tropicalis) SIMILARITY: Belongs to the peptidase M14 family. FUNCTION: Metallocarboxypeptidase that mediates deglutamylation of tubulin and non-tubulin target proteins. Catalyzes the removal of polyglutamate side chains present on the gamma-carboxyl group of glutamate residues within the C-terminal tail of alpha- and beta-tubulin. Cleaves alpha- and gamma-linked polyglutamate tubulin side-chain, as well as the branching point glutamate. Also catalyzes the removal of alpha-linked glutamate residues from the carboxy-terminus of alpha-tubulin. KEYWORDS: Carboxypeptidase;Cytoplasm;Cytoskeleton;Hydrolase;Metal-binding;Metalloprotease;Nucleus;Protease;Reference proteome;Zinc SUBCELLULAR LOCATION: Cytoplasm, cytosol Nucleus Cytoplasm, cytoskeleton, spindle Midbody " B0R367,PROTEIN NAMES: Transducer protein MpcT (Membrane potential change transducer protein) PROTEIN FAMILY: Methyl-accepting chemotaxis (MCP) protein family ORGANISM: Halobacterium salinarum (strain ATCC 29341 / DSM 671 / R1) SIMILARITY: Belongs to the methyl-accepting chemotaxis (MCP) protein family. FUNCTION: Mediates bacteriorhodopsin- and halorhodopsin-dependent photoresponses by detecting membrane potential changes. Probably transduces the signal to the histidine kinase CheA. KEYWORDS: Cell membrane;Chemotaxis;Membrane;Methylation;Repeat;Transducer;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein B0XT72,"PROTEIN NAMES: 1,3-beta-glucanosyltransferase gel1 (Glucan elongating glucanosyltransferase 1) PROTEIN FAMILY: Glycosyl hydrolase 72 family ORGANISM: Aspergillus fumigatus (strain CBS 144.89 / FGSC A1163 / CEA10) (Neosartorya fumigata) SIMILARITY: Belongs to the glycosyl hydrolase 72 family. FUNCTION: Splits internally a 1,3-beta-glucan molecule and transfers the newly generated reducing end (the donor) to the non-reducing end of another 1,3-beta-glucan molecule (the acceptor) forming a 1,3-beta linkage, resulting in the elongation of 1,3-beta-glucan chains in the cell wall. Involved in cell wall morphogenesis. KEYWORDS: Cell membrane;Direct protein sequencing;Disulfide bond;Glycoprotein;GPI-anchor;Lipoprotein;Membrane;Signal;Transferase SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor, GPI-anchor. " B0Y0P7,"PROTEIN NAMES: Adenylyltransferase and sulfurtransferase uba4 (Common component for nitrate reductase and xanthine dehydrogenase protein F) (Ubiquitin-like protein activator 4) [Includes: Molybdopterin-synthase adenylyltransferase (Adenylyltransferase uba4) (Sulfur carrier protein MOCS2A adenylyltransferase); Molybdopterin-synthase sulfurtransferase (Sulfur carrier protein MOCS2A sulfurtransferase) (Sulfurtransferase uba4)] PROTEIN FAMILY: HesA/MoeB/ThiF family, UBA4 subfamily ORGANISM: Aspergillus fumigatus (strain CBS 144.89 / FGSC A1163 / CEA10) (Neosartorya fumigata) SIMILARITY: In the N-terminal section; belongs to the HesA/MoeB/ThiF family. UBA4 subfamily. FUNCTION: Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers urm1 and mocs2a. Its N-terminus first activates urm1 and mocs2a as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to urm1 and mocs2a to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as a nucleophile towards urm1 and mocs2a. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; nfs1 probably acting as a sulfur donor for thiocarboxylation reactions (By similarity). PATHWAY: tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.; PATHWAY: Cofactor biosynthesis; molybdopterin biosynthesis. KEYWORDS: ATP-binding;Cytoplasm;Metal-binding;Molybdenum cofactor biosynthesis;Multifunctional enzyme;Nucleotide-binding;Transferase;tRNA processing;Zinc SUBCELLULAR LOCATION: Cytoplasm " B0YJ81,"PROTEIN NAMES: Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 1 (3-hydroxyacyl-CoA dehydratase 1) (HACD1) (Cementum-attachment protein) (CAP) (Protein-tyrosine phosphatase-like member A) PROTEIN FAMILY: Very long-chain fatty acids dehydratase HACD family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the very long-chain fatty acids dehydratase HACD family. FUNCTION: [Isoform 1]: Catalyzes the third of the four reactions of the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process, allows the addition of two carbons to the chain of long- and very long-chain fatty acids/VLCFAs per cycle. This enzyme catalyzes the dehydration of the 3-hydroxyacyl-CoA intermediate into trans-2,3-enoyl-CoA, within each cycle of fatty acid elongation. Thereby, it participates in the production of VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators.; FUNCTION: [Isoform 2]: In tooth development, may play a role in the recruitment and the differentiation of cells that contribute to cementum formation. May also bind hydroxyapatite and regulate its crystal nucleation to form cementum. PATHWAY: Lipid metabolism; fatty acid biosynthesis. KEYWORDS: Alternative splicing;Developmental protein;Disease variant;Endoplasmic reticulum;Fatty acid biosynthesis;Fatty acid metabolism;Glycoprotein;Lipid biosynthesis;Lipid metabolism;Lyase;Membrane;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: [Isoform 1]: Endoplasmic reticulum membrane ; Multi-pass membrane protein MISCELLANEOUS: [Isoform 2]: Catalytically inactive since it lacks the active site but may have an alternative function." B0ZB56,"PROTEIN NAMES: Xanthohumol 4-O-methyltransferase (Desmethylxanthohumol 6'-O-methyltransferase) (Isoliquiritigenin 2'-O-methyltransferase) (O-methyltransferase 2) (HlOMT2) PROTEIN FAMILY: Class I-like SAM-binding methyltransferase superfamily, Cation-independent O-methyltransferase family ORGANISM: Humulus lupulus (European hop) SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O-methyltransferase family. FUNCTION: Involved in the biosynthesis of prenylated phenolics natural products which contribute to the bitter taste of beer and display broad biological activities (Probable). O-methyltransferase with a low substrate selectivity. Methylates chalconaringenin, desmethylxanthohumol, xanthohumol, isoliquiritigenin, butein, 2',4-dihydroxychalcone, resveratrol, genistein and guaiacol. Catalyzes the biosynthesis of 2',4'-dihydroxy-4,6'-dimethoxy-3'-prenylchalcone (4-O-methylxanthohumol). PATHWAY: Secondary metabolite biosynthesis. KEYWORDS: Cytoplasm;Methyltransferase;S-adenosyl-L-methionine;Transferase SUBCELLULAR LOCATION: Cytoplasm " B1H234,"PROTEIN NAMES: Leucine-rich repeat transmembrane protein FLRT3 (Fibronectin-like domain-containing leucine-rich transmembrane protein 3) ORGANISM: Rattus norvegicus (Rat) FUNCTION: Functions in cell-cell adhesion, cell migration and axon guidance, exerting an attractive or repulsive role depending on its interaction partners. Plays a role in the spatial organization of brain neurons. Plays a role in vascular development in the retina (By similarity). Plays a role in cell-cell adhesion via its interaction with ADGRL3 and probably also other latrophilins that are expressed at the surface of adjacent cells (By similarity). Interaction with the intracellular domain of ROBO1 mediates axon attraction towards cells expressing NTN1. Mediates axon growth cone collapse and plays a repulsive role in neuron guidance via its interaction with UNC5B, and possibly also other UNC-5 family members (By similarity). Promotes neurite outgrowth (in vitro) (By similarity). Mediates cell-cell contacts that promote an increase both in neurite number and in neurite length. Plays a role in the regulation of the density of glutamaergic synapses. Plays a role in fibroblast growth factor-mediated signaling cascades. Required for normal morphogenesis during embryonic development, but not for normal embryonic patterning. Required for normal ventral closure, headfold fusion and definitive endoderm migration during embryonic development. Required for the formation of a normal basement membrane and the maintenance of a normal anterior visceral endoderm during embryonic development (By similarity). KEYWORDS: Cell adhesion;Cell junction;Cell membrane;Cell projection;Cytoplasmic vesicle;Developmental protein;Disulfide bond;Endoplasmic reticulum;Glycoprotein;Leucine-rich repeat;Membrane;Reference proteome;Repeat;Secreted;Signal;Synapse;Synaptosome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass membrane protein Synapse, synaptosome Postsynaptic density Cell projection, dendrite Synapse Presynaptic cell membrane Cell projection, axon Cell projection, growth cone membrane Cytoplasmic vesicle Endoplasmic reticulum membrane Cell junction, focal adhesion Secreted Note=Detected on dendritic punctae that colocalize in part with glutamaergic synapses, but not with GABAergic synapses. Proteolytic cleavage in the juxtamembrane region gives rise to a shedded ectodomain (By similarity). " B1NF18,PROTEIN NAMES: Salutaridine synthase (Cytochrome P450 719B1) PROTEIN FAMILY: Cytochrome P450 family ORGANISM: Papaver somniferum (Opium poppy) SIMILARITY: Belongs to the cytochrome P450 family. FUNCTION: Cytochrome P450 monooxygenase involved in biosynthesis of morphinan-type benzylisoquinoline and opiate alkaloids natural products. Catalyzes the formation of the morphinan alkaloid salutaridine by intramolecular phenol oxidation of (R)-reticuline without the incorporation of oxygen into the product. Can also use (R)-norreticuline as substrate. KEYWORDS: Endoplasmic reticulum;Heme;Iron;Membrane;Metal-binding;Monooxygenase;Oxidoreductase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Single-pass membrane protein B1Q005,"PROTEIN NAMES: Ghrelin O-acyltransferase (Membrane-bound O-acyltransferase domain-containing protein 4) PROTEIN FAMILY: Membrane-bound acyltransferase family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the membrane-bound acyltransferase family. FUNCTION: Catalyzes ghrelin acylation at 'Ser-3' using preferentially octanoyl-CoA, hexanoyl-CoA and decanoyl-CoA as acyl-CoA donors leading to ghrelin activity (By similarity). In vitro uses also acyl-CoA donors of different lengths from short-chain (C2) to long-chain fatty acids (C16) knowing that acyl-CoA donors from butanoyl-CoA (C4) to dodecanoyl-CoA (C12) are more efficient compared to longer acyl-CoA donors, such as myristoyl-CoA (C14) and palmitoyl-CoA (C16) that are not efficient (By similarity). KEYWORDS: Acyltransferase;Endoplasmic reticulum;Membrane;Reference proteome;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein " B1W019,"PROTEIN NAMES: (+)-caryolan-1-ol synthase ((+)-beta-caryophyllene synthase) PROTEIN FAMILY: Terpene synthase family ORGANISM: Streptomyces griseus subsp. griseus (strain JCM 4626 / NBRC 13350) SIMILARITY: Belongs to the terpene synthase family. FUNCTION: Sesquiterpene cyclase that first catalyzes the cyclization of farnesyl diphosphate (FPP) to the bicyclic sesquiterpene (+)-beta-caryophyllene intermediate, and then its conversion to (+)-caryolan-1-ol via a second cyclization and the addition of a water molecule. PATHWAY: Secondary metabolite biosynthesis; terpenoid biosynthesis. KEYWORDS: Hydrolase;Lyase;Magnesium;Manganese;Metal-binding MISCELLANEOUS: A study of the reaction mechanism indicates that (+)-caryolan-1-ol is synthesized by a proton attack on the C-8/C-9 double bond of (+)-beta-caryophyllene." B1WC61,"PROTEIN NAMES: Complex I assembly factor ACAD9, mitochondrial (Acyl-CoA dehydrogenase family member 9) (ACAD-9) PROTEIN FAMILY: Acyl-CoA dehydrogenase family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the acyl-CoA dehydrogenase family. FUNCTION: As part of the MCIA complex, primarily participates in the assembly of the mitochondrial complex I and therefore plays a role in oxidative phosphorylation. This moonlighting protein has also a dehydrogenase activity toward a broad range of substrates with greater specificity for long-chain unsaturated acyl-CoAs. However, in vivo, it does not seem to play a primary role in fatty acid oxidation. In addition, the function in complex I assembly is independent of the dehydrogenase activity of the protein. KEYWORDS: Acetylation;FAD;Flavoprotein;Membrane;Mitochondrion;Mitochondrion inner membrane;Oxidoreductase;Phosphoprotein;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Mitochondrion inner membrane ; Peripheral membrane protein ; Matrix side Note=Essentially associated with membranes. " B1YAL1,"PROTEIN NAMES: Fructose-1,6-bisphosphate aldolase/phosphatase (FBP A/P) (FBP aldolase/phosphatase) PROTEIN FAMILY: FBP aldolase/phosphatase family ORGANISM: Pyrobaculum neutrophilum (strain DSM 2338 / JCM 9278 / NBRC 100436 / V24Sta) (Thermoproteus neutrophilus) SIMILARITY: Belongs to the FBP aldolase/phosphatase family. FUNCTION: Catalyzes two subsequent steps in gluconeogenesis: the aldol condensation of dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (GA3P) to fructose-1,6-bisphosphate (FBP), and the dephosphorylation of FBP to fructose-6-phosphate (F6P). PATHWAY: Carbohydrate biosynthesis; gluconeogenesis. KEYWORDS: 3D-structure;Carbohydrate metabolism;Gluconeogenesis;Hydrolase;Lyase;Magnesium;Metal-binding;Schiff base " B2D6M2,PROTEIN NAMES: Protein lin-61 (Abnormal cell lineage protein 61) ORGANISM: Caenorhabditis elegans FUNCTION: Synthetic multivulva class B (synMuvB) protein required to repress the induction of vulval development by Ras signaling. Unlike other synMuv proteins it does not associate with the multiprotein DRM complex and the NuRD-like complex. Interaction with methylated histone H3 is essential for vulva development. It has a role in maintaining genome stability. KEYWORDS: Alternative splicing;Chromosome;Nucleus;Reference proteome;Repeat SUBCELLULAR LOCATION: Nucleus Chromosome B2DBE9,"PROTEIN NAMES: Geranylgeranyl pyrophosphate synthase D (GGPP synthase D) (GGPPSase) ((2E,6E)-farnesyl diphosphate synthase D) (Dimethylallyltranstransferase D) (Farnesyl diphosphate synthase D) (Farnesyltranstransferase D) (Geranylgeranyl diphosphate synthase D) (Geranyltranstransferase D) PROTEIN FAMILY: FPP/GGPP synthase family ORGANISM: Phomopsis amygdali (Fusicoccum amygdali) SIMILARITY: Belongs to the FPP/GGPP synthase family. FUNCTION: Catalyzes the trans-addition of the 3 molecules of isopentenyl diphosphate (IPP) onto dimethylallyl diphosphate (DMAPP) to form geranylgeranyl pyrophosphate (GGDP). PATHWAY: Isoprenoid biosynthesis; farnesyl diphosphate biosynthesis; farnesyl diphosphate from geranyl diphosphate and isopentenyl diphosphate: step 1/1.; PATHWAY: Isoprenoid biosynthesis; geranyl diphosphate biosynthesis; geranyl diphosphate from dimethylallyl diphosphate and isopentenyl diphosphate: step 1/1.; PATHWAY: Isoprenoid biosynthesis; geranylgeranyl diphosphate biosynthesis; geranylgeranyl diphosphate from farnesyl diphosphate and isopentenyl diphosphate: step 1/1. KEYWORDS: Cytoplasm;Isoprene biosynthesis;Magnesium;Metal-binding;Transferase SUBCELLULAR LOCATION: Cytoplasm. " B2FHL8,"PROTEIN NAMES: Polysaccharide lyase (PL) (Alginate lyase) (Endolytic polysaccharide lyase) (Hyaluronate lyase) (Multifunctional polysaccharide lyase) (Poly-beta-D-glucuronate lyase) PROTEIN FAMILY: Polysaccharide lyase 5 family ORGANISM: Stenotrophomonas maltophilia (strain K279a) SIMILARITY: Belongs to the polysaccharide lyase 5 family. FUNCTION: Polysaccharide lyase that catalyzes the depolymerization of several anionic polysaccharides via a beta-elimination mechanism. Exhibits broad substrate specificity, catalyzing the degradation of not only alginate and poly-beta-D-mannuronate (poly-ManA), but poly-beta-D-glucuronate (poly-GlcA or poly-GlcUA) and hyaluronate (HA) as well. The oligosaccharide products formed by enzymatic cleavage are comprised mainly of disaccharides, with a lower abundance of trimers and pentamers. Is not active on poly-D-galacturonate, heparin and heparin sulfate. KEYWORDS: 3D-structure;Carbohydrate metabolism;Cell outer membrane;Lipoprotein;Lyase;Membrane;Palmitate;Polysaccharide degradation;Reference proteome;Signal SUBCELLULAR LOCATION: Cell outer membrane ; Lipid-anchor " B2FSW8,"PROTEIN NAMES: Alginate lyase (Exolytic alginate lyase) (Exolytic polysaccharide lyase) PROTEIN FAMILY: Polysaccharide lyase 17 family ORGANISM: Stenotrophomonas maltophilia (strain K279a) SIMILARITY: Belongs to the polysaccharide lyase 17 family. FUNCTION: Polysaccharide lyase that catalyzes the depolymerization of alginate via a beta-elimination mechanism, cleaving the beta-1,4 glycosidic bond between two adjacent sugar residues. Acts specifically on alginate and each of its block structures, with highest activity toward poly-beta-D-mannuronate (poly-ManA). Shows an exolytic mode of action, producing unsaturated monomers. Displays a very low activity against poly-beta-D-glucuronate (poly-GlcA), and is not active on poly-alpha-D-galacturonate, hyaluronan, heparin, heparan sulfate and chondroitin sulfate. KEYWORDS: Carbohydrate metabolism;Lyase;Metal-binding;Periplasm;Polysaccharide degradation;Reference proteome;Signal;Zinc SUBCELLULAR LOCATION: Periplasm " B2IZD3,"PROTEIN NAMES: Bacterial dynamin-like protein (BDLP) PROTEIN FAMILY: TRAFAC class dynamin-like GTPase superfamily, Dynamin/Fzo/YdjA family, Mitofusin subfamily ORGANISM: Nostoc punctiforme (strain ATCC 29133 / PCC 73102) SIMILARITY: Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family. Mitofusin subfamily. FUNCTION: Dynamin-related GTPase probably involved in membrane remodeling. Lipid and nucleotide-binding are thought to induce a large intramolecular rearrangement, leading to assembly on lipid bilayers and possible membrane curving. In the presence of the non-hydrolyzable GTP analog GMP-PNP self-assembles on a lipid bilayer; this does not stimulate subsequent GTPase activity. Does not bind lipids in the presence of GDP; perhaps GTP hydrolysis disrupts membrane-binding. KEYWORDS: 3D-structure;Cell inner membrane;Cell membrane;Coiled coil;GTP-binding;Hydrolase;Lipid-binding;Membrane;Nucleotide-binding;Reference proteome SUBCELLULAR LOCATION: Cell inner membrane ; Peripheral membrane protein Note=Probably inserts into the outer leaflet of the membrane only (Probable). Forms foci localized in the cell periphery, and occasionally in the cell interior. " B2J528,"PROTEIN NAMES: Phenylalanine ammonia-lyase PROTEIN FAMILY: PAL/histidase family ORGANISM: Nostoc punctiforme (strain ATCC 29133 / PCC 73102) SIMILARITY: Belongs to the PAL/histidase family. FUNCTION: Catalyzes the non-oxidative deamination of L-phenylalanine to form trans-cinnamic acid, the first step in the phenylpropanoid pathway. PATHWAY: Phenylpropanoid metabolism; trans-cinnamate biosynthesis; trans-cinnamate from L-phenylalanine: step 1/1. KEYWORDS: 3D-structure;Cytoplasm;Lyase;Phenylalanine catabolism;Phenylpropanoid metabolism;Reference proteome SUBCELLULAR LOCATION: Cytoplasm " B2RHG1,"PROTEIN NAMES: Minor fimbrium subunit Mfa1 (Pg-II fim a) PROTEIN FAMILY: Bacteroidetes fimbrillin superfamily ORGANISM: Porphyromonas gingivalis (strain ATCC 33277 / DSM 20709 / CIP 103683 / JCM 12257 / NCTC 11834 / 2561) SIMILARITY: Belongs to the bacteroidetes fimbrillin superfamily. FUNCTION: Structural subunit of the minor fimbriae. These filamentous pili are attached to the cell surface; they mediate biofilm formation, adhesion onto host cells and onto other bacteria that are part of the oral microbiome. They play an important role in invasion of periodontal tissues and are recognized as major virulence factors. Mfa1 orthologs from different strains have highly divergent sequences, and this correlates with pathogenicity (Probable). KEYWORDS: 3D-structure;Cell outer membrane;Direct protein sequencing;Fimbrium;Lipoprotein;Membrane;Palmitate;Signal;Virulence SUBCELLULAR LOCATION: Fimbrium Cell outer membrane Note=Probably synthesized as a palmitoylated precursor. Efficient export to the outer membrane and integration into fimbriae requires lipidation and subsequent proteolytic removal of the lipidated propeptide (Probable). MISCELLANEOUS: The name (minor fimbrium subunit) does not indicate the abundance of the protein, but is derived from the greater length of the major fimbriae. In strain ATCC 33277 and strain ATCC BAA-1703 / FDC 381, major fimbriae are 300 - 1600 nM in length and about 5 nm in diameter. In contrast, minor fimbriae are only about 80 - 120 nm long. This length difference is observed only in a small number of strains, including strain ATCC 33277 and strain ATCC BAA-1703 / FDC 381, and is due to a loss of function mutation in FimB, a protein that restricts fimbrial length in other strains." B2RHG2,"PROTEIN NAMES: Minor fimbrium anchoring subunit Mfa2 (Minor fimbrial antigen 2) PROTEIN FAMILY: Bacteroidetes fimbrillin superfamily, FimB/Mfa2 family ORGANISM: Porphyromonas gingivalis (strain ATCC 33277 / DSM 20709 / CIP 103683 / JCM 12257 / NCTC 11834 / 2561) SIMILARITY: Belongs to the bacteroidetes fimbrillin superfamily. FimB/Mfa2 family. FUNCTION: Anchoring subunit of the minor fimbriae. Regulates fimbrial length. These filamentous pili are attached to the cell surface; they mediate biofilm formation, adhesion onto host cells and onto other bacteria that are part of the oral microbiome. Fimbriae of P.gingivalis are major virulence factors (Probable). KEYWORDS: 3D-structure;Cell outer membrane;Lipoprotein;Membrane;Palmitate;Signal;Virulence SUBCELLULAR LOCATION: Cell outer membrane ; Lipid-anchor MISCELLANEOUS: The name (minor fimbrium subunit) does not indicate the abundance of the protein, but is derived from the greater length of the major fimbriae. In strain ATCC 33277 and strain ATCC BAA-1703 / FDC 381, major fimbriae are 300 - 1600 nM in length and about 5 nm in diameter. In contrast, minor fimbriae are only about 80 - 120 nm long. This length difference is observed only in a small number of strains, including strain ATCC 33277 and strain ATCC BAA-1703 / FDC 381, and is due to a loss of function mutation in FimB, a protein that restricts fimbrial length in other strains." B2RPY5,"PROTEIN NAMES: G-protein coupled receptor 161 PROTEIN FAMILY: G-protein coupled receptor 1 family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the G-protein coupled receptor 1 family. FUNCTION: Key negative regulator of Shh signaling, which promotes the processing of GLI3 into GLI3R during neural tube development. Recruited by TULP3 and the IFT-A complex to primary cilia and acts as a regulator of the PKA-dependent basal repression machinery in Shh signaling by increasing cAMP levels, leading to promote the PKA-dependent processing of GLI3 into GLI3R and repress the Shh signaling. In presence of SHH, it is removed from primary cilia and is internalized into recycling endosomes, preventing its activity and allowing activation of the Shh signaling. Its ligand is unknown. KEYWORDS: Alternative splicing;Cataract;Cell membrane;Cell projection;Cilium;Developmental protein;Disulfide bond;G-protein coupled receptor;Glycoprotein;Membrane;Receptor;Reference proteome;Transducer;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell projection, cilium membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Note=Mainly localizes to primary cilium in a TULP3 and IFT-A complex-dependent manner. In presence of SHH, it is removed from primary cilia and is internalized into recycling endosomes and is apparently not degraded. " B2RQL2,"PROTEIN NAMES: Storkhead-box protein 1 ORGANISM: Mus musculus (Mouse) FUNCTION: Involved in regulating the levels of reactive oxidative species and reactive nitrogen species and in mitochondrial homeostasis in the placenta (By similarity). Required for regulation of inner ear epithelial cell proliferation via the AKT signaling pathway. Involved in cell cycle regulation by binding to the CCNB1 promoter, up-regulating its expression and promoting mitotic entry (By similarity). Induces phosphorylation of MAPT/tau (By similarity). KEYWORDS: Activator;Cell cycle;Cell division;Cytoplasm;Cytoskeleton;DNA-binding;Mitosis;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus Cytoplasm Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Note=In epithelial cells, diffusely expressed in the cytoplasm, particularly in peri-membrane cortical regions. Concentrated at centrosomes during metaphase (By similarity). " B2RS91,"PROTEIN NAMES: RNA polymerase I-specific transcription initiation factor RRN3 PROTEIN FAMILY: RRN3 family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the RRN3 family. FUNCTION: Required for efficient transcription initiation by RNA polymerase I. Required for the formation of the competent pre-initiation complex (PIC). KEYWORDS: Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus, nucleolus " B2RXZ1,"PROTEIN NAMES: Poly(A)-specific ribonuclease PNLDC1 (PARN-like domain-containing protein 1) (Poly(A)-specific ribonuclease domain-containing protein 1) PROTEIN FAMILY: CAF1 family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the CAF1 family. FUNCTION: 3'-exoribonuclease that has a preference for poly(A) tails of mRNAs, thereby efficiently degrading poly(A) tails. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs and is also used to silence certain maternal mRNAs translationally during oocyte maturation and early embryonic development. May act as a regulator of multipotency in embryonic stem cells. Is a critical factor for proper spermatogenesis, involved in pre-piRNAs processing to generate mature piRNAs (By similarity). KEYWORDS: Endoplasmic reticulum;Exonuclease;Hydrolase;Magnesium;Membrane;Metal-binding;Nonsense-mediated mRNA decay;Nuclease;Reference proteome;RNA-binding;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Single-pass membrane protein Note=Localizes mainly in the endoplasmic reticulum. " B2RYG6,"PROTEIN NAMES: Ubiquitin thioesterase OTUB1 (Deubiquitinating enzyme OTUB1) (OTU domain-containing ubiquitin aldehyde-binding protein 1) (Otubain-1) (Ubiquitin-specific-processing protease OTUB1) PROTEIN FAMILY: Peptidase C65 family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the peptidase C65 family. FUNCTION: Hydrolase that can specifically remove compared to 'Lys-48'-linked conjugated ubiquitin from proteins and plays an important regulatory role at the level of protein turnover by preventing degradation (By similarity). Regulator of T-cell anergy, a phenomenon that occurs when T-cells are rendered unresponsive to antigen rechallenge and no longer respond to their cognate antigen (By similarity). Acts via its interaction with RNF128/GRAIL (By similarity). Surprisingly, it regulates RNF128-mediated ubiquitination, but does not deubiquitinate polyubiquitinated RNF128 (By similarity). Deubiquitinates estrogen receptor alpha (ESR1) (By similarity). Mediates deubiquitination of 'Lys-48'-linked polyubiquitin chains, but not 'Lys-63'-linked polyubiquitin chains (By similarity). Not able to cleave di-ubiquitin (By similarity). Also capable of removing NEDD8 from NEDD8 conjugates, but with a much lower preference compared to 'Lys-48'-linked ubiquitin (By similarity).; FUNCTION: Plays a key non-catalytic role in DNA repair regulation by inhibiting activity of RNF168, an E3 ubiquitin-protein ligase that promotes accumulation of 'Lys-63'-linked histone H2A and H2AX at DNA damage sites. Inhibits RNF168 independently of ubiquitin thioesterase activity by binding and inhibiting UBE2N/UBC13, the E2 partner of RNF168, thereby limiting spreading of 'Lys-63'-linked histone H2A and H2AX marks. Inhibition occurs by binding to free ubiquitin: free ubiquitin acts as an allosteric regulator that increases affinity for UBE2N/UBC13 and disrupts interaction with UBE2V1. The OTUB1-UBE2N/UBC13-free ubiquitin complex adopts a configuration that mimics a cleaved 'Lys48'-linked di-ubiquitin chain. Acts as a regulator of mTORC1 and mTORC2 complexes. When phosphorylated at Tyr-26, acts as an activator of the mTORC1 complex by mediating deubiquitination of RPTOR via a non-catalytic process: acts by binding and inhibiting the activity of the ubiquitin-conjugating enzyme E2 (UBE2D1/UBCH5A, UBE2W/UBC16 and UBE2N/UBC13), thereby preventing ubiquitination of RPTOR. Can also act as an inhibitor of the mTORC1 and mTORC2 complexes in response to amino acids by mediating non-catalytic deubiquitination of DEPTOR. KEYWORDS: Acetylation;Adaptive immunity;Cytoplasm;DNA damage;DNA repair;Hydrolase;Immunity;Phosphoprotein;Protease;Reference proteome;Thiol protease;Ubl conjugation pathway SUBCELLULAR LOCATION: Cytoplasm " B2UP57,"PROTEIN NAMES: Beta-hexosaminidase Amuc_2018 (Beta-N-acetylhexosaminidase Am2301) PROTEIN FAMILY: Glycosyl hydrolase 20 family ORGANISM: Akkermansia muciniphila (strain ATCC BAA-835 / DSM 22959 / JCM 33894 / BCRC 81048 / CCUG 64013 / CIP 107961 / Muc) SIMILARITY: Belongs to the glycosyl hydrolase 20 family. FUNCTION: Hydrolyzes terminal GlcNAc residues from terminally unbranched N-glycans and from chitobiose. Hydrolyzes beta-1,6-linked N-acetylglucosamine and beta-1,4-linked N-acetylgalactosamine from pNP-alpha-GalNAc[beta1,3Gal]beta1,6GlcNAc and pNP-beta-GlcNAc-beta1,4-GalNAc substrates, respectively, as well as beta-1,2-linked N-acetylglucosamine units from the non-reducing end of N-glycans. Hydrolyzes GlcNAc residues linked to alpha1,3- or alpha1,6-mannose branch, but has low activity on substrates with more than one GlcNAc residue on one of the mannose branches. Releases terminal GlcNAc moieties from the N-glycopeptide Gly-Glu-Asn-(GlcNAc2Man3GlcNAc2)-Arg with high efficiency. Has moderate hydrolytic activity on the chitobiose moiety of N-glycopeptide substrate Gly-Glu-Asn-(GlcNAc2)-Arg. Does not hydrolyze GlcNAc residues from N-glycan structures bearing a bisecting GlcNAc moiety (beta1,4-linked GlcNAc to the beta1,4-linked core mannose). Potentially capable of cleaving the specific glycoside linkages in the process of mucin degradation in human intestinal tract (Probable). Hydrolyzes synthetic substrate pNP-beta-GlcNAc with high activity and pNP-beta-GalNAc to a lesser extent. Does not hydrolyze pNP-beta-glucose, pNP-beta-galactose, pNP-alpha-glucose, pNP-alpha-galactose, pNP-alpha-GlcNAc or pNP-alpha-fucose. KEYWORDS: 3D-structure;Carbohydrate metabolism;Glycosidase;Hydrolase;Metal-binding;Polysaccharide degradation;Reference proteome;Signal;Zinc " B2ZXD5,"PROTEIN NAMES: Golgi pH regulator (Protein GPR89) PROTEIN FAMILY: Golgi pH regulator family ORGANISM: Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) SIMILARITY: Belongs to the Golgi pH regulator (TC 1.A.38) family. FUNCTION: Voltage-gated channel that enables the transfer of anions such as iodide, chloride, bromide and fluoride which may function in counter-ion conductance and participates in Golgi acidification. Plays a role in lymphocyte development, probably by acting as a RABL3 effector in hematopoietic cells (By similarity). KEYWORDS: Glycoprotein;Golgi apparatus;Ion channel;Ion transport;Membrane;Protein transport;Transmembrane;Transmembrane helix;Transport;Voltage-gated channel SUBCELLULAR LOCATION: Golgi apparatus membrane ; Multi-pass membrane protein " B3A003,"PROTEIN NAMES: Lysozyme 3 (1,4-beta-N-acetylmuramidase 3) (Invertebrate-type lysozyme 3) (cv-lysozyme 3) PROTEIN FAMILY: Glycosyl hydrolase 22 family, Type-I lysozyme subfamily ORGANISM: Crassostrea virginica (Eastern oyster) SIMILARITY: Belongs to the glycosyl hydrolase 22 family. Type-I lysozyme subfamily. FUNCTION: Has antibacterial activity against the Gram-negative bacterium E.coli. No antibacterial activity detected against the Gram-negative bacterium V.vulnificus. KEYWORDS: Antibiotic;Antimicrobial;Bacteriolytic enzyme;Direct protein sequencing;Disulfide bond;Glycosidase;Hydrolase;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted " B3GSH5,"PROTEIN NAMES: Acetylserotonin O-methyltransferase (Hydroxyindole O-methyltransferase) PROTEIN FAMILY: Class I-like SAM-binding methyltransferase superfamily, Cation-independent O-methyltransferase family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O-methyltransferase family. FUNCTION: Catalyzes the transfer of a methyl group onto N-acetylserotonin, producing melatonin (N-acetyl-5-methoxytryptamine). PATHWAY: Aromatic compound metabolism; melatonin biosynthesis; melatonin from serotonin: step 1/2. KEYWORDS: Lipid metabolism;Melatonin biosynthesis;Methyltransferase;Reference proteome;S-adenosyl-L-methionine;Transferase MISCELLANEOUS: Pineal melatonin synthesis is severely compromised in most inbred strains. In many inbred strains, genetic defects in ASMT have been identified. Melatonin production may have an impact on gonadal development, testis development being significantly promoted in melatonin-deficient C57BL/6J x Mus musculus molossinus animals." B3H5K9,"PROTEIN NAMES: Protein NEDD1 (Neural precursor cell expressed developmentally down-regulated protein 1 homolog) (Protein GCP-WD) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Regulates microtubules organization in a centrosome-independent manner. Required for the spindle to be positioned correctly and for the function of gamma-tubulin in organizing phragmoplast microtubules. Component of active gamma-tubulin ring complexes (gamma-TuRCs) associated with cortical microtubules in interphase cells. Mediates gamma-TuRC recruitment to the nucleation sites and is important for determining the ratio of branched to parallel nucleation. May mediate the localization of GCP2 and GCP3 to the nuclear envelope. KEYWORDS: Alternative splicing;Centromere;Chromosome;Coiled coil;Cytoplasm;Cytoskeleton;Kinetochore;Nucleus;Reference proteome;Repeat;WD repeat SUBCELLULAR LOCATION: Nucleus envelope Chromosome, centromere, kinetochore Cytoplasm, cytoskeleton, phragmoplast Cytoplasm, cytoskeleton, microtubule organizing center Note=First detected in prophase on the nuclear envelope, where it appeared to cap the future spindle poles. Later detected along kinetochore microtubules (MTs) of the metaphase spindle, with more prominent signals toward the poles. In anaphase, detected with the shortening kinetochore fibers. In the developing phragmoplast, localized mainly toward the minus end of MTs. " B3LF48,"PROTEIN NAMES: EH domain-containing protein 2 (AtEHD2) PROTEIN FAMILY: TRAFAC class dynamin-like GTPase superfamily, Dynamin/Fzo/YdjA family, EHD subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family. EHD subfamily. FUNCTION: Involved in endocytosis negative regulation, probably by influencing actin organization. Acts in early endocytic membrane fusion and membrane trafficking of recycling endosomes. Exhibits an inhibitory effect on endocytosis when over-expressed. KEYWORDS: Alternative splicing;Calcium;Cell membrane;Coiled coil;Cytoplasm;Endocytosis;Endosome;GTP-binding;Hydrolase;Membrane;Metal-binding;Nucleotide-binding;Nucleus;Reference proteome;Repeat SUBCELLULAR LOCATION: Endosome membrane ; Peripheral membrane protein Cell membrane ; Peripheral membrane protein ; Cytoplasmic side Nucleus Cytoplasm " B4F6I3,"PROTEIN NAMES: Ferroptosis suppressor protein 1 (FSP1) (Apoptosis-inducing factor homologous mitochondrion-associated inducer of death) (AMID) (p53-responsive gene 3 protein) PROTEIN FAMILY: FAD-dependent oxidoreductase family ORGANISM: Xenopus tropicalis (Western clawed frog) (Silurana tropicalis) SIMILARITY: Belongs to the FAD-dependent oxidoreductase family. FUNCTION: A NAD(P)H-dependent oxidoreductase that acts as a key inhibitor of ferroptosis. At the plasma membrane, catalyzes reduction of coenzyme Q/ubiquinone-10 to ubiquinol-10, a lipophilic radical-trapping antioxidant that prevents lipid oxidative damage and consequently ferroptosis. Acts in parallel to GPX4 to suppress phospholipid peroxidation and ferroptosis. This anti-ferroptotic function is independent of cellular glutathione levels. Also acts as a potent radical-trapping antioxidant by mediating warfarin-resistant vitamin K reduction in the canonical vitamin K cycle: catalyzes NAD(P)H-dependent reduction of vitamin K (phylloquinone, menaquinone-4 and menadione) to hydroquinone forms. Hydroquinones act as potent radical-trapping antioxidants inhibitor of phospholipid peroxidation and ferroptosis. May play a role in mitochondrial stress signaling. Upon oxidative stress, associates with the lipid peroxidation end product 4-hydroxy-2-nonenal (HNE) forming a lipid adduct devoid of oxidoreductase activity, which then translocates from mitochondria into the nucleus triggering DNA damage and cell death. KEYWORDS: Apoptosis;Cell membrane;Cytoplasm;FAD;Flavoprotein;Lipid droplet;Lipoprotein;Membrane;Mitochondrion;Myristate;Nucleus;Oxidoreductase;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Lipid droplet Cell membrane ; Lipid-anchor Cytoplasm Mitochondrion membrane Nucleus " B4F7C5,"PROTEIN NAMES: Leucine-rich repeat transmembrane neuronal protein 4 PROTEIN FAMILY: LRRTM family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the LRRTM family. FUNCTION: May play a role in the development and maintenance of the nervous system (By similarity). Exhibits strong synaptogenic activity, restricted to excitatory presynaptic differentiation. KEYWORDS: Cell membrane;Glycoprotein;Leucine-rich repeat;Membrane;Postsynaptic cell membrane;Reference proteome;Repeat;Signal;Synapse;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein Postsynaptic cell membrane ; Single-pass type I membrane protein " B4GJC1,"PROTEIN NAMES: Protein adenylyltransferase Fic (De-AMPylase Fic) PROTEIN FAMILY: Fic family ORGANISM: Drosophila persimilis (Fruit fly) SIMILARITY: Belongs to the fic family. FUNCTION: Protein that can both mediate the addition of adenosine 5'-monophosphate (AMP) to specific residues of target proteins (AMPylation), and the removal of the same modification from target proteins (de-AMPylation), depending on the context (By similarity). The side chain of Glu-261 determines which of the two opposing activities (AMPylase or de-AMPylase) will take place (By similarity). Acts as a key regulator of the unfolded protein response (UPR) by mediating AMPylation or de-AMPylation of Hsc70-3/BiP. In unstressed cells, acts as an adenylyltransferase by mediating AMPylation of Hsc70-3/BiP at 'Thr-518', thereby inactivating it. In response to endoplasmic reticulum stress, acts as a phosphodiesterase by mediating removal of ATP (de-AMPylation) from Hsc70-3/BiP at 'Thr-518', leading to restore HSPA5/BiP activity (By similarity). KEYWORDS: ATP-binding;Hydrolase;Membrane;Nucleotide-binding;Nucleotidyltransferase;Reference proteome;Repeat;TPR repeat;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Single-pass membrane protein " B4MXR8,"PROTEIN NAMES: Serine/threonine-protein kinase PLK4 (Polo-like kinase 4) (PLK-4) (Serine/threonine-protein kinase SAK) PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family, CDC5/Polo subfamily ORGANISM: Drosophila willistoni (Fruit fly) SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily. FUNCTION: Serine/threonine-protein kinase that plays a central role in centriole duplication. Able to trigger procentriole formation on the surface of the mother centriole cylinder, using mother centriole as a platform, leading to the recruitment of centriole biogenesis proteins such as sas-6. When overexpressed, it is able to induce centrosome amplification through the simultaneous generation of multiple procentrioles adjoining each parental centriole during S phase. Centrosome amplification following overexpression can initiate tumorigenesis, highlighting the importance of centrosome regulation in cancers (By similarity). KEYWORDS: ATP-binding;Cytoplasm;Cytoskeleton;Kinase;Nucleotide-binding;Reference proteome;Serine/threonine-protein kinase;Transferase;Ubl conjugation SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole " B5BLW5,"PROTEIN NAMES: Arylesterase (A-esterase) (Paraoxonase) PROTEIN FAMILY: 'GDXG' lipolytic enzyme family ORGANISM: Saccharolobus solfataricus (Sulfolobus solfataricus) SIMILARITY: Belongs to the 'GDXG' lipolytic enzyme family. FUNCTION: Has a broad substrate specificity. Hydrolyzes various p-nitrophenyl phosphates, aromatic esters and p-nitrophenyl fatty acids in vitro. Most active against paraoxon, phenyl acetate and p-nitrophenyl caproate (C6), respectively. Has also tributyrinase activity, but shows no hydrolytic activity toward other triacylglycerols including tricaprylin, trimyristin, tripalmitin or triolein in vitro. KEYWORDS: 3D-structure;Direct protein sequencing;Hydrolase;Serine esterase " B5HDJ6,"PROTEIN NAMES: Selina-4(15),7(11)-diene synthase ((2E,6E)-farnesyl diphosphate cyclizing) (Selinadiene synthase) (SdS) (Terpene synthase) (Type I terpene cyclase) PROTEIN FAMILY: Terpene synthase family ORGANISM: Streptomyces pristinaespiralis (strain ATCC 25486 / DSM 40338 / CBS 914.69 / JCM 4507 / NBRC 13074 / NRRL 2958 / 5647) SIMILARITY: Belongs to the terpene synthase family. FUNCTION: Catalyzes the conversion of (2E,6E)-farnesyl diphosphate (FPP) to yield the bicyclic sesquiterpene selina-4(15),7(11)-diene via a 1,10-cyclization, which requires the abstraction of the pyrophosphate from FPP leading to a (E,E)-germacradienyl cation. The only accepted substrate is (2E,6E)-farnesyl diphosphate (FPP). PATHWAY: Secondary metabolite biosynthesis; terpenoid biosynthesis. KEYWORDS: 3D-structure;Lyase;Magnesium;Metal-binding;Reference proteome " B5L3X1,"PROTEIN NAMES: UDP-Gal:alpha-D-GlcNAc-diphosphoundecaprenol beta-1,4-galactosyltransferase PROTEIN FAMILY: Glycosyltransferase 26 family ORGANISM: Shigella boydii SIMILARITY: Belongs to the glycosyltransferase 26 family. FUNCTION: Galactosyltransferase that adds one galactose residue in the beta-1-4 linkage to GlcNAc-alpha-pyrophosphate-lipid in the biosynthesis of the O-polysaccharide repeating unit of the O antigen. PATHWAY: Bacterial outer membrane biogenesis; LPS O-antigen biosynthesis. KEYWORDS: Glycosyltransferase;Lipopolysaccharide biosynthesis;Transferase " B5TVM2,"PROTEIN NAMES: Immunoglobulin-like domain-containing receptor 2 (Angulin-3) (Lisch-like protein) PROTEIN FAMILY: Immunoglobulin superfamily, LISCH7 family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the immunoglobulin superfamily. LISCH7 family. FUNCTION: May be involved in ER stress pathways with effects on lipid homeostasis and insulin secretion. With ILDR1 and LSR, involved in the maintain of the epithelial barrier function through the recruitment of MARVELD2/tricellulin to tricellular tight junctions. Also functions as a B7-like protein family member expressed on immune cells and inflamed tissue and with T-cell inhibitory activity. In the inner ear, may regulate alternative pre-mRNA splicing via binding to TRA2A, TRA2B and SRSF1. KEYWORDS: Alternative splicing;Cell junction;Disulfide bond;Endoplasmic reticulum;Immunoglobulin domain;Membrane;Methylation;Nucleus;Phosphoprotein;Reference proteome;Signal;Tight junction;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Single-pass type I membrane protein Cell junction, tight junction Nucleus " B5XK69,"PROTEIN NAMES: Oleate hydratase (Fatty acid double bond hydratase) (Fatty acid hydratase) (Linoleate hydratase) (Myosin cross-reactive antigen) (MCRA) PROTEIN FAMILY: Oleate hydratase family ORGANISM: Streptococcus pyogenes serotype M49 (strain NZ131) SIMILARITY: Belongs to the oleate hydratase family. FUNCTION: Catalyzes the hydration of oleate at its cis-9-double bond to yield 10-hydroxyoctadecanoate, probably in the (R) configuration, and of linoleate at its cis-9- and cis-12-double bond to yield 10-hydroxy-12-octadecenoate and 10,13-dihydroxyoctadecanoate. Is not active on trans-double bonds and esterified fatty acids as substrate; is only active on cis-9- and/or cis-12-double bond of C16 and C18 fatty acids without any trans-configurations, producing 10-hydroxy and 10,13-dihydroxy derivatives. Appears to play a role in oleic acid detoxification and bacterial virulence. PATHWAY: Lipid metabolism; fatty acid metabolism. KEYWORDS: Detoxification;FAD;Fatty acid metabolism;Flavoprotein;Lipid metabolism;Lyase MISCELLANEOUS: Unsaturated fatty acids are toxic for many bacteria due to deteriorating effect on bacterial cellular membrane and disruption of bacterial fatty acid synthesis. The hydration of unsaturated fatty acids is suggested to be a detoxification mechanism and a survival strategy for living in fatty acid-rich environments." B5XRB0,"PROTEIN NAMES: NADH:fumarate oxidoreductase (Fumarate reductase) (FRD) PROTEIN FAMILY: FAD-dependent oxidoreductase 2 family, FRD/SDH subfamily ORGANISM: Klebsiella pneumoniae (strain 342) SIMILARITY: Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily. FUNCTION: Catalyzes the anaerobic reduction of fumarate to succinate. Uses NADH as the inherent electron donor in this process. Is involved in anaerobic fumarate respiration in K.pneumoniae. KEYWORDS: Cytoplasm;FAD;Flavoprotein;FMN;Oxidoreductase;Phosphoprotein SUBCELLULAR LOCATION: Cytoplasm " B6A879,"PROTEIN NAMES: Chitinase 2 PROTEIN FAMILY: Glycosyl hydrolase 18 family ORGANISM: Yersinia entomophaga SIMILARITY: Belongs to the glycosyl hydrolase 18 family. FUNCTION: Part of an orally active toxin complex (TC) with strong insecticidal effects on larvae of the Coleoptera Costelytra zealandica, Acrossidius tasmania and Adoryphorus couloni and some Lepidoptera larvae. The TC has an endochitinase activity (Probable). This subunit might aid infection by degradation of the larval peritrophic membrane (Probable). KEYWORDS: 3D-structure;Carbohydrate metabolism;Chitin degradation;Glycosidase;Hydrolase;Polysaccharide degradation;Secreted;Virulence SUBCELLULAR LOCATION: Secreted Note=Secreted when grown at 25 degrees Celsius or less, but not when grown at 30 or 37 degrees Celsius. " B6A8C7,"PROTEIN NAMES: T-cell-interacting, activating receptor on myeloid cells protein 1 (OSCAR-like transcript-2 protein) (OLT-2) ORGANISM: Homo sapiens (Human) FUNCTION: May act as receptor (By similarity). Negatively regulates TCR-mediated CD4(+) T cell proliferation and activation, possibly by binding an unknown ligand on the T cell surface. Enhances Toll-like receptor-mediated production of pro-inflammatory cytokines by macrophages and neutrophils (By similarity). KEYWORDS: Adaptive immunity;Alternative splicing;Cell membrane;Disulfide bond;Glycoprotein;Immunity;Immunoglobulin domain;Innate immunity;Membrane;Receptor;Reference proteome;Repeat;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein " B7IE18,PROTEIN NAMES: Lipid II flippase MurJ PROTEIN FAMILY: MurJ/MviN family ORGANISM: Thermosipho africanus (strain TCF52B) SIMILARITY: Belongs to the MurJ/MviN family. FUNCTION: Involved in peptidoglycan biosynthesis. Transports lipid-linked peptidoglycan precursors from the inner to the outer leaflet of the cytoplasmic membrane. PATHWAY: Cell wall biogenesis; peptidoglycan biosynthesis. KEYWORDS: 3D-structure;Cell inner membrane;Cell membrane;Cell shape;Cell wall biogenesis/degradation;Membrane;Peptidoglycan synthesis;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell inner membrane ; Multi-pass membrane protein B7JBP8,"PROTEIN NAMES: Sulfide-quinone reductase (SQR) (Sulfide:quinone oxidoreductase) PROTEIN FAMILY: SQRD family ORGANISM: Acidithiobacillus ferrooxidans (strain ATCC 23270 / DSM 14882 / CIP 104768 / NCIMB 8455) (Ferrobacillus ferrooxidans (strain ATCC 23270)) SIMILARITY: Belongs to the SQRD family. FUNCTION: Catalyzes the oxidation of hydrogen sulfide, with the help of a quinone. Consecutive reaction cycles lead to the accumulation of a polysulfide product on the active site Cys residues; these products are released when they exceed a critical length, typically as cyclooctasulfur. KEYWORDS: 3D-structure;FAD;Flavoprotein;Membrane;Nucleotide-binding;Oxidoreductase;Quinone;Reference proteome SUBCELLULAR LOCATION: Membrane ; Peripheral membrane protein " A0A1W6QDI7,"PROTEIN NAMES: Miltiradiene synthase KSL1, chloroplastic (Kaurene synthase 1) (IrKSL1) (Terpene synthase 4) (IrTPS4) PROTEIN FAMILY: Terpene synthase family ORGANISM: Isodon rubescens (Rabdosia rubescens) SIMILARITY: Belongs to the terpene synthase family. FUNCTION: Involved in the biosynthesis of ent-kaurene diterpenoids natural products such as oridonin, miltiradiene, eriocalyxin B and nezukol, known to exhibit antitumor, anti-inflammatory and antibacterial activities. Catalyzes the conversion of (+)-copalyl diphosphate ((+)-CPP) to miltiradiene. PATHWAY: Secondary metabolite biosynthesis; terpenoid biosynthesis. KEYWORDS: Alternative splicing;Chloroplast;Lyase;Magnesium;Metal-binding;Plastid;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast MISCELLANEOUS: Abietane diterpenoids (e.g. miltiradiene, abietatriene and ferruginol) accumulate specifically in the periderm of roots. The ent-kaurene diterpenoid oridonin, main constituent of Isodon rubescens, accumulates in leaves." A0A1Z2R986,"PROTEIN NAMES: Endoglin ORGANISM: Danio rerio (Zebrafish) (Brachydanio rerio) FUNCTION: Vascular endothelium glycoprotein that plays an important role in the regulation of angiogenesis. Required for normal structure and integrity of adult vasculature. Important for endothelial cell shape changes in response to blood flow, which drive vascular remodeling and establishment of normal vascular morphology during angiogenesis. KEYWORDS: Angiogenesis;Cell membrane;Disulfide bond;Glycoprotein;Membrane;Reference proteome;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein " A0A2I7G3B3,"PROTEIN NAMES: Alcohol dehydrogenase 2 (TcADH2) (Trans-chrysanthemal synthase) PROTEIN FAMILY: Zinc-containing alcohol dehydrogenase family, Class-IV subfamily ORGANISM: Tanacetum cinerariifolium (Dalmatian daisy) (Chrysanthemum cinerariifolium) SIMILARITY: Belongs to the zinc-containing alcohol dehydrogenase family. Class-IV subfamily. FUNCTION: Component of the monoterpenoid pyrethrins biosynthesis; pyrethrins are widely used plant-derived pesticide. Mediates the conversion of trans-chrysanthemol into trans-chrysanthemal. PATHWAY: Isoprenoid biosynthesis. KEYWORDS: Isoprene biosynthesis;Metal-binding;NAD;Oxidoreductase;Zinc " A0A2L0VXR5,"PROTEIN NAMES: Geranylgeranyl pyrophosphate synthase (GGPP synthase) (GGPPSase) (GGS) ((2E,6E)-farnesyl diphosphate synthase) (Dimethylallyltranstransferase) (Farnesyl diphosphate synthase) (Farnesyltranstransferase) (Geranylgeranyl diphosphate synthase) (Geranyltranstransferase) (Pleuromutilin biosynthetic cluster protein synthesis protein G) PROTEIN FAMILY: FPP/GGPP synthase family ORGANISM: Clitopilus passeckerianus (Pleurotus passeckerianus) SIMILARITY: Belongs to the FPP/GGPP synthase family. FUNCTION: Geranylgeranyl pyrophosphate synthase; part of the gene cluster that mediates the biosynthesis of pleuromutilin, a tricyclic diterpene showing antibacterial properties. The geranylgeranyl diphosphate (GGPP) synthase catalyzes the first step in pleuromutilin biosynthesis. GGPP is then substrate of the premutilin synthase (PS) to yield premutilin. Premutilin synthase is a bifunctional enzyme composed of the fusion of a class II diterpene cyclase (DTC) and a class I diterpene synthase (DTS), with the corresponding domains and active sites containing characteristic aspartate-rich motifs. GGPP is first converted to mutildienyl-diphosphate (MPP) at the class II DTC site. MPP is subsequently further cyclized at the class I DTS site, followed by a 1,5-hydride shift and addition of water prior to terminating deprotonation, to yield premutilin. In addition to the aforementioned GGPP synthase and bifunctional diterpene synthase, the cluster contains also three cytochrome P450 monooxygenases, a short-chain alcohol dehydrogenase, and an acyltransferase, involved in the conversion of premutilin to pleuromutilin. The cytochrome P450 monooxygenases P450-1 and P450-2 hydroxylate premutilin at C-11 and C-3, respectively, producing 11-hydroxypremutilin and 3-hydroxypremutilin (By similarity). The combination of the actions of both ple5 and ple6 leads to the production of 3,11-dihydroxypremutilin (By similarity). The short chain dehydrogenase SDR further converts 3,11-dihydroxypremutilin into mutilin (By similarity). The acetyltransferase ATF then acetylates mutilin to produce 14-O-acetylmutilin (By similarity). Finally, the cytochrome P450 monooxygenase P450-3 catalyzes hydroxylation on the alpha position of the acetyl side chain of 14-O-acetylmutilin to yield pleuromutilin (By similarity). PATHWAY: Secondary metabolite biosynthesis; terpenoid biosynthesis. KEYWORDS: Antibiotic biosynthesis;Isoprene biosynthesis;Magnesium;Metal-binding;Transferase " A0A060A682,"PROTEIN NAMES: Hapless 2 (Generative cell specific-1) PROTEIN FAMILY: HAP2/GCS1 family ORGANISM: Tetrahymena thermophila SIMILARITY: Belongs to the HAP2/GCS1 family. FUNCTION: During fertilization, required for the formation of intercellular membrane pores and subsequent exchange of gametic pronuclei between cells. Probably initiates the formation of intercellular membrane pores by inserting part of its extracellular domain into the cell membrane of the adjoining cell in the mating pair. Mating requires the presence of HAP2 on at least one of the two cells. Mating efficiency is high when HAP2 is present on both cells, and is strongly reduced when HAP2 is present on only one of the two cells. KEYWORDS: Cell junction;Cell membrane;Disulfide bond;Fertilization;Lipid-binding;Membrane;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein Cell junction Note=Detected at the mating junction. MISCELLANEOUS: HAP2/GCS1 family members mediate membrane fusion between gametes in a broad range of eukaryotes, ranging from algae and higher plants to protozoans and cnidaria, suggesting they are derived from an ancestral gamete fusogen. They function similar to viral fusogens, by inserting part of their extracellular domain into the lipid bilayer of an adjoining cell." A0A068J840,PROTEIN NAMES: UDP-glycosyltransferase 1 (UGTPg1) PROTEIN FAMILY: UDP-glycosyltransferase family ORGANISM: Panax ginseng (Korean ginseng) SIMILARITY: Belongs to the UDP-glycosyltransferase family. FUNCTION: Component of the dammarane-type triterpene saponins (e.g. ginsenosides or panaxosides) biosynthetic pathway. Glycosyltransferase that catalyzes the biosynthesis of ginsenoside F1 from protopanaxatriol (PPT). Triggers C20-OH glycosylation of ginsenoside Rg3 to produce ginsenoside Rd. Mediates the conversion of protopanaxadiol (PPD) to the ginsenoside compound K. catalyzes the production of 20S-O-beta-(D-glucosyl)-dammarenediol II form dammarenediol II (DM). PATHWAY: Secondary metabolite biosynthesis; terpenoid biosynthesis. KEYWORDS: Glycosyltransferase;Isoprene biosynthesis;Transferase A0A075TRK9,"PROTEIN NAMES: Patulin synthase (Dehydrogenase patE) (Patulin biosynthesis cluster protein E) PROTEIN FAMILY: GMC oxidoreductase family ORGANISM: Penicillium expansum (Blue mold rot fungus) SIMILARITY: Belongs to the GMC oxidoreductase family. FUNCTION: Patulin synthase; part of the gene cluster that mediates the biosynthesis of patulin, an acetate-derived tetraketide mycotoxin produced by several fungal species that shows antimicrobial properties against several bacteria. PatE catalyzes the last step of the pathway which is the conversion of E-ascladiol to patulin. The pathway begins with the synthesis of 6-methylsalicylic acid by the polyketide synthase (PKS) patK via condensation of acetate and malonate units. The 6-methylsalicylic acid decarboxylase patG then catalyzes the decarboxylation of 6-methylsalicylic acid to yield m-cresol (also known as 3-methylphenol). These first reactions occur in the cytosol. The intermediate m-cresol is then transported into the endoplasmic reticulum where the cytochrome P450 monooxygenase patH converts it to m-hydroxybenzyl alcohol, which is further converted to gentisyl alcohol by the cytochrome P450 monooxygenase patI. The oxidoreductases patJ and patO further convert gentisyl alcohol to isoepoxydon in the vacuole. PatN catalyzes then the transformation of isoepoxydon into phyllostine. The cluster protein patF is responsible for the conversion from phyllostine to neopatulin whereas the alcohol dehydrogenase patD converts neopatulin to E-ascladiol. The steps between isoepoxydon and E-ascladiol occur in the cytosol, and E-ascladiol is probably secreted to the extracellular space by one of the cluster-specific transporters patC or patM. Finally, the secreted patulin synthase patE catalyzes the conversion of E-ascladiol to patulin (Probable). PATHWAY: Mycotoxin biosynthesis; patulin biosynthesis. KEYWORDS: 3D-structure;Cell wall;Cytoplasm;FAD;Flavoprotein;Glycoprotein;Oxidoreductase;Reference proteome;Secreted;Signal;Vacuole SUBCELLULAR LOCATION: Cytoplasm, cell cortex Vacuole Secreted Secreted, cell wall " A0A087X1C5,"PROTEIN NAMES: Putative cytochrome P450 2D7 PROTEIN FAMILY: Cytochrome P450 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the cytochrome P450 family. FUNCTION: May be responsible for the metabolism of many drugs and environmental chemicals that it oxidizes. It may be involved in the metabolism of codeine to morphine. However, another study could not confirm it. KEYWORDS: Cytoplasm;Glycoprotein;Heme;Iron;Membrane;Metal-binding;Mitochondrion;Monooxygenase;Oxidoreductase;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Multi-pass membrane protein Cytoplasm Mitochondrion " A0A096P8D3,"PROTEIN NAMES: Isocitrate dehydrogenase (NAD(+)), mitochondrial (OtIDH) PROTEIN FAMILY: Isocitrate and isopropylmalate dehydrogenases family ORGANISM: Ostreococcus tauri SIMILARITY: Belongs to the isocitrate and isopropylmalate dehydrogenases family. FUNCTION: Performs an essential role in the oxidative function of the tricarboxylic acid cycle and respiration (Probable). Catalyzes the decarboxylation of isocitrate to produce 2-oxoglutarate and generate NADH to provide electrons for energy production (Probable). KEYWORDS: 3D-structure;Magnesium;Manganese;Metal-binding;Mitochondrion;NAD;Oxidoreductase;Reference proteome;Transit peptide;Tricarboxylic acid cycle SUBCELLULAR LOCATION: Mitochondrion " A0A097PTA8,"PROTEIN NAMES: Fungal defensin copsin PROTEIN FAMILY: Invertebrate defensin family ORGANISM: Coprinopsis cinerea (Inky cap fungus) (Hormographiella aspergillata) SIMILARITY: Belongs to the invertebrate defensin family. FUNCTION: Antimicrobial peptide that acts against Gram-positive bacteria (Listeria spp., Enterococcus spp., B.subtilis, B.anthracis, P.aeruginosa). Is not active against Gram-negative bacteria. It selectively inhibits peptidoglycan biosynthesis through complex formation with the cell wall precursor lipid II (1:1 molar ratio), probably anchoring lipid II to the membrane, thus inhibiting cell wall synthesis. The interaction with lipid II involves the third position of the pentapeptide. Shows bactericidal activity at about 2-fold minimal inhibitory concentrations (MIC), but does not form pore across the membrane. KEYWORDS: 3D-structure;Antibiotic;Antimicrobial;Cleavage on pair of basic residues;Defensin;Direct protein sequencing;Disulfide bond;Lipid-binding;Membrane;Pyrrolidone carboxylic acid;Secreted;Signal;Target cell membrane;Target membrane SUBCELLULAR LOCATION: Secreted Target cell membrane Note=specific localization at active cell wall synthesis sites. " A0A0A1H8I4,"PROTEIN NAMES: Aconitate isomerase (AI) ORGANISM: Pseudomonas sp FUNCTION: Involved in assimilation of trans-aconitic acid. Preference for cis-aconitic acid is 14-fold higher than for trans-aconitic acid. Not active on intermediates of tricarboxylic acid (TCA) cycle including citric acid, succinic acid, fumaric acid, and 2-oxoglutaric acid or on other dicarboxilic acids including itaconic acid, formic acid, citraconic acid or maleic acid. KEYWORDS: Direct protein sequencing;Isomerase;Signal " A0A0A7HIF0,"PROTEIN NAMES: CRISPR system Cms endoribonuclease Csm3 (Csm3 RNase) (CRISPR type III A-associated RAMP protein Csm3) PROTEIN FAMILY: CRISPR-associated Csm3 family ORGANISM: Streptococcus thermophilus SIMILARITY: Belongs to the CRISPR-associated Csm3 family. FUNCTION: CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). The type III-A Csm effector complex binds crRNA and acts as a crRNA-guided RNase, DNase and cyclic oligoadenylate synthase; binding of target RNA cognate to the crRNA is required for all activities. In a heterologous host this Csm effector complex restricts ssRNA phage MS2, suggesting it may target RNA viruses in vivo.; FUNCTION: Csm functions as a non-specific ssDNase. Base-pairing between crRNA and target RNA to form a ternary Csm complex activates a ssDNase activity; target RNA cleavage suppresses the ssDNase, a temporal control that prevents uncontrolled DNA degradation. Viral RNA transcripts probably tether the Csm complex to the viral genome, recruiting Cas10 ssDNA activity which is able to degrade DNA in the transcription bubble, spatially controlling the DNase activity.; FUNCTION: This subunit has the target ssRNA endonuclease activity; it cleaves multiple sites in the target RNA at 6 nucleotide intervals. The number of cleavage sites in the target RNA correlates with the number of Csm3 subunits in the Csm effector complex. In the Csm complex target RNA and ssDNA are cleaved simultaneously, although RNase activity (of Csm3) is much faster. RNA cleavage by Csm3 is not required for ssDNase activity as Csm complex with inactive Csm3 still has ssDNase activity; however as the cleaved target RNA products dissociate away ssDNase activity decreases. KEYWORDS: 3D-structure;Antiviral defense;Endonuclease;Hydrolase;Nuclease;RNA-binding MISCELLANEOUS: Encoded in a type III-A CRISPR locus." A0A0A7HIX6,"PROTEIN NAMES: CRISPR system endoribonuclease Csm6 (CRISPR type III-A associated protein Csm6-1) PROTEIN FAMILY: CRISPR-associated Csm6 family ORGANISM: Streptococcus thermophilus SIMILARITY: Belongs to the CRISPR-associated Csm6 family. FUNCTION: CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). The type III-A Csm complex binds crRNA and acts as a crRNA-guided RNase, DNase and cyclic oligoadenylate synthase; binding of target RNA cognate to the crRNA is required for all activities. In a heterologous host this Csm effector complex restricts ssRNA phage MS2, suggesting it may target RNA viruses in vivo. This protein is not part of the Csm complex.; FUNCTION: Csm functions as a non-specific ssDNase. Base-pairing between crRNA and target RNA to form a ternary Csm complex activates a ssDNase activity; target RNA cleavage suppresses the ssDNase, a temporal control that prevents uncontrolled DNA degradation. Viral RNA transcripts probably tether the Csm complex to the viral genome, recruiting Cas10 ssDNA activity which is able to degrade DNA in the transcription bubble, spatially controlling the DNase activity.; FUNCTION: A single-strand-specific endoribonuclease (ssRNase) that is approximately 1000-fold stimulated by cyclic oligoadenylate (cOA); although several species of cOA are synthesized by this organism only cyclic hexaadenylate (cA6) stimulates the ssRNase activity. Cleaves preferentially within GA or AA dinucleotides, although the presence of cA6 broadens the preference. Linear oligoadenylates do not activate the RNase. KEYWORDS: Antiviral defense;Endonuclease;Hydrolase;Nuclease;Nucleotide-binding;RNA-binding MISCELLANEOUS: Encoded in a type III-A CRISPR locus." A0A0B0QJR1,"PROTEIN NAMES: tRNA nuclease HepT (Toxin HEPN) (tRNA nuclease HEPN) PROTEIN FAMILY: HepT RNase toxin family ORGANISM: Aphanizomenon flos-aquae (strain 2012/KM1/D3) SIMILARITY: Belongs to the HepT RNase toxin family. FUNCTION: Toxic component of a type VII toxin-antitoxin (TA) system. Upon cloning in E.coli inhibits cell growth for several hours; eventually cells recover and start growing. Cleaves the last 4 nucleotides from the tRNA acceptor stem (shown in vitro with E.coli tRNA-Glu(UUC)); only cleaves intact tRNA. Has no activity on mRNA. Neutralized by coexpression with cognate antitoxin MntA, which is due to di-AMPylation of the RNase. KEYWORDS: 3D-structure;Endonuclease;Hydrolase;Nuclease;Nucleotide-binding;Phosphoprotein;Toxin-antitoxin system MISCELLANEOUS: Part of a locus that includes subtype I-D CRISPR-Cas genes." A0A0B7P9G0,PROTEIN NAMES: Unextended protein (Putative metal transporter uex) PROTEIN FAMILY: ACDP family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the ACDP family. FUNCTION: Probable metal transporter (By similarity). Acts downstream of PRL-1 and protects the nervous system against olfactory carbon dioxide stimulation. KEYWORDS: 3D-structure;CBS domain;Cell membrane;Glycoprotein;Ion transport;Membrane;Reference proteome;Repeat;Signal;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein A0A0G2JXN2,"PROTEIN NAMES: Tripartite motif-containing protein 46 PROTEIN FAMILY: TRIM/RBCC family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the TRIM/RBCC family. FUNCTION: Microtubule-associated protein that is involved in the formation of parallel microtubule bundles linked by cross-bridges in the proximal axon. Required for the uniform orientation and maintenance of the parallel microtubule fascicles, which are important for efficient cargo delivery and trafficking in axons. Thereby also required for proper axon specification, the establishment of neuronal polarity and proper neuronal migration. KEYWORDS: Cell projection;Coiled coil;Cytoplasm;Cytoskeleton;Metal-binding;Phosphoprotein;Reference proteome;Repeat;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cell projection, axon Cytoplasm, cytoskeleton Note=Microtubule-associated. Localizes to the proximal part of the axon. " A0A0G2KQY6,"PROTEIN NAMES: Metal cation symporter ZIP14 (Solute carrier family 39 member 14) (Zrt- and Irt-like protein 14) (ZIP-14) PROTEIN FAMILY: ZIP transporter family ORGANISM: Danio rerio (Zebrafish) (Brachydanio rerio) SIMILARITY: Belongs to the ZIP transporter (TC 2.A.5) family. FUNCTION: Broad-scope metal ion transporter with a preference for zinc uptake. Also mediates cellular uptake of nontransferrin-bound iron.; FUNCTION: Electroneutral transporter of the plasma membrane mediating the cellular uptake of the divalent metal cations zinc, manganese and iron that are important for tissue homeostasis, metabolism, development and immunity. Functions as an energy-dependent symporter, transporting through the membranes an electroneutral complex composed of a divalent metal cation and two bicarbonate anions (By similarity). Beside these endogenous cellular substrates, can also import cadmium a non-essential metal which is cytotoxic and carcinogenic (By similarity). KEYWORDS: Cell membrane;Endosome;Ion transport;Lysosome;Membrane;Reference proteome;Signal;Transmembrane;Transmembrane helix;Transport;Zinc;Zinc transport SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Apical cell membrane ; Multi-pass membrane protein Basolateral cell membrane ; Multi-pass membrane protein Early endosome membrane ; Multi-pass membrane protein Late endosome membrane ; Multi-pass membrane protein Lysosome membrane ; Multi-pass membrane protein " A0A0H2V871,"PROTEIN NAMES: Apo-salmochelin esterase (Enterobactin hydrolase IroE) PROTEIN FAMILY: Esterase D family ORGANISM: Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC) SIMILARITY: Belongs to the esterase D family. FUNCTION: Catalyzes the hydrolysis of both the apo and Fe3(+)-bound forms of enterobactin (Ent), monoglucosyl-C-Ent (MGE), diglucosyl-C-Ent (DGE) and triglucosyl-C-Ent (TGE). It prefers apo siderophores as substrates and hydrolyzes the Fe3(+)-bound siderophores very inefficiently. Tends to hydrolyze the trilactone just once to produce linearized trimers. May hydrolyze and linearize some or all of apo enterobactins while they are being exported. KEYWORDS: 3D-structure;Cell inner membrane;Cell membrane;Hydrolase;Membrane;Reference proteome;Serine esterase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell inner membrane ; Single-pass membrane protein ; Periplasmic side " A0A0H2V8B5,"PROTEIN NAMES: NAD(+) hydrolase TcpC (NADP(+) hydrolase TcpC) (TIR domain-containing protein in E.coli) (tcpC) ORGANISM: Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC) FUNCTION: Virulence factor that suppresses host Toll-like receptor (TLR)-mediated cytokine production upon infection, thereby increasing bacterial burden in the urinary tract and promoting renal tissue damage. Acts as a NAD(+) hydrolase (NADase) by catalyzing cleavage of NAD(+) into ADP-D-ribose (ADPR) and nicotinamide. Also able to hydrolyze NADP(+), but not other NAD(+)-related molecules. KEYWORDS: Hydrolase;Membrane;NAD;Reference proteome;Secreted;Transmembrane;Transmembrane helix;Virulence SUBCELLULAR LOCATION: Secreted Membrane ; Single-pass membrane protein " A0A0H2VDN9,"PROTEIN NAMES: Secretory immunoglobulin A-binding protein EsiB ORGANISM: Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC) FUNCTION: Upon host (human neutrophil) infection interferes with productive FCAR signaling, inhibiting secreted IgA (SIgA) effector functions and probably avoiding neutrophil activation. Inhibits the SIgA-mediated oxidative burst by neutrophils, decreases generation of ROS (reactive oxygen species) by neutrophils and reduces chemotaxis by neutrophils, all of which are SIgA effector functions used to stimulate the immune response. Does not block SIgA-binding to its receptor (FCAR) on neutrophils, but it decreases SIgA-stimulated phosphorylation of cytoplasmic proteins, including phospholipase C-gamma and MAP kinases, all actions that may be advantageous to the pathogen. KEYWORDS: 3D-structure;Direct protein sequencing;Magnesium;Metal-binding;Reference proteome;Repeat;Signal;Virulence SUBCELLULAR LOCATION: Cell surface Note=Accumulates at 1 cell pole in the bladder of mice infected with this strain, in overexpressing bacteria the protein is found all over the cell surface but not in the secreted fraction. Human blood sera from clinical patients with urinary tract infections reacts with antibodies to this protein. MISCELLANEOUS: Preferentially encoded in ExPEC strains (extraintestinal pathogenic E.coli)." A0A0H2WWV6,PROTEIN NAMES: Poly(ribitol-phosphate) alpha-N-acetylglucosaminyltransferase (WTA GlcNAc-transferase) PROTEIN FAMILY: Glycosyltransferase group 1 family ORGANISM: Staphylococcus aureus (strain COL) SIMILARITY: Belongs to the glycosyltransferase group 1 family. FUNCTION: Attaches N-acetyl-alpha-D-glucosamine residues to poly(RboP)-wall teichoic acids (WTAs). PATHWAY: Cell wall biogenesis; poly(ribitol phosphate) teichoic acid biosynthesis. KEYWORDS: 3D-structure;Cell wall biogenesis/degradation;Cytoplasm;Glycosyltransferase;Teichoic acid biosynthesis;Transferase SUBCELLULAR LOCATION: Cytoplasm A0A0H3JPC6,PROTEIN NAMES: Poly(ribitol-phosphate) beta-N-acetylglucosaminyltransferase TarS (Beta-O-GlcNAc transferase) (Beta-O-GlcNAc-WTA transferase) (WTA glycosyltransferase) (Wall teichoic acid beta-glycosyltransferase) PROTEIN FAMILY: Glycosyltransferase 2 family ORGANISM: Staphylococcus aureus (strain Mu50 / ATCC 700699) SIMILARITY: Belongs to the glycosyltransferase 2 family. FUNCTION: Attaches beta-O-GlcNAc (beta-O-N-acetyl-D-glucosamine) residues to the C4 position of poly(RboP)-wall teichoic acids (WTAs). Mediates beta-lactam resistance in methicillin resistant Staphylococcus aureus (MRSA) strains. PATHWAY: Cell wall biogenesis; poly(ribitol phosphate) teichoic acid biosynthesis. KEYWORDS: 3D-structure;Antibiotic resistance;Cell wall biogenesis/degradation;Glycosyltransferase;Manganese;Metal-binding;Teichoic acid biosynthesis;Transferase;Virulence MISCELLANEOUS: TarS is a unique target for compounds used in combination with beta-lactams to treat MRSA infections. A0A0H3MDW1,"PROTEIN NAMES: Atypical response regulator protein ChxR (Transcriptional regulatory protein) ORGANISM: Chlamydia trachomatis serovar L2 (strain ATCC VR-902B / DSM 19102 / 434/Bu) FUNCTION: May be a global positive regulator of transcription. Binds a cis-acting element of its own promoter DNA sequence and is hence probably also involved in its own transcription activation. The recognition sequence is 5'-WHGAWNH-N(3-5)-WHGAWNH-3', where W is A/T, H is C/A/T, N is G/C/A/T and the linker length in the middle is 3 to 5 nucleotides. KEYWORDS: 3D-structure;Activator;DNA-binding;Transcription;Transcription regulation;Two-component regulatory system " A0A0K0JFP3,"PROTEIN NAMES: Hexokinase (BmHK) PROTEIN FAMILY: Hexokinase family ORGANISM: Brugia malayi (Filarial nematode worm) SIMILARITY: Belongs to the hexokinase family. FUNCTION: Active against glucose, fructose, mannose, maltose and galactose. PATHWAY: Carbohydrate metabolism; hexose metabolism.; PATHWAY: Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 1/4. KEYWORDS: ATP-binding;Glycolysis;Kinase;Nucleotide-binding;Reference proteome;Transferase " A0A0K3AWM6,"PROTEIN NAMES: Protein mom-5 PROTEIN FAMILY: G-protein coupled receptor Fz/Smo family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the G-protein coupled receptor Fz/Smo family. FUNCTION: Receptor for Wnt proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of gsk-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes (Probable). A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as pkc seems to be required for Wnt-mediated inactivation of gsk-3 kinase (Probable). Both pathways seem to involve interactions with G-proteins (Probable). Required in embryonic development for the correct positioning and orientation of the mitotic spindles and division planes in blastomere cells. During early embryonic cell divisions, directs the asymmetric positioning of transcription factors such as pop-1 and dsh-2 in daughter cells in order to determine cell fate specification. Acts redundantly with other Wnt receptors such as lin-17 to control vulval precursor cell specification and also the polarity of different cell types including distal tip cells, seam cells, AVG interneurons and P-cells and their descendants. Plays a role in the migration of cell types including distal tip cells and the QR neuroblast descendants, QR.p and QR.pa during larval development. Negatively regulates the unc-6/Netrin receptors unc-5 and unc-40 to control distal tip cell polarity and migration. Acts through ced-5/DOCK180 and ced-10/Rac to control both distal tip cell migration and the phagocytic clearance of apoptotic cell corpses. Furthermore, it is also required for the migration and axon guidance of the different neuronal cell types including CAN, ALM, HSN and the two mechanosensory neurons AVM and PVM. Mediates Wnt receptor cfz-2 in directing ALM migration, but may also act redundantly with the Wnt receptors cfz-2 and mig-1 to direct the migration of other neuronal cell types including CAN and HSN. Mediates Wnt ligand egl-20 in the control of the anterior-posterior axon guidance of AVM and PVM neurons. KEYWORDS: Alternative splicing;Cell membrane;Developmental protein;Disulfide bond;Endosome;Glycoprotein;Membrane;Receptor;Reference proteome;Signal;Transmembrane;Transmembrane helix;Wnt signaling pathway SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Early endosome Note=Uniformaly localized along the cell membrane throughout the cell cycle, but occasionally enriched at prophase towards the posterior pole of the cell. Sequestered from the cell membrane to endosomes by plr-1 to prevent Wnt signaling. " A0A0P0VIP0,"PROTEIN NAMES: L-type lectin-domain containing receptor kinase S.7 (OsLecRK-S.7) (Protein DEFECTIVE IN APERTURE FORMATION 1) (OsDAF1) PROTEIN FAMILY: Leguminous lectin family; Protein kinase superfamily, Ser/Thr protein kinase family ORGANISM: Oryza sativa subsp. japonica (Rice) SIMILARITY: In the N-terminal section; belongs to the leguminous lectin family.; SIMILARITY: In the C-terminal section; belongs to the protein kinase superfamily. Ser/Thr protein kinase family. FUNCTION: Legume-lectin receptor-like kinase required for normal pollen development and male fertility. Regulates pollen exine assembly and aperture development. Plays a critical role in annulus formation, and may participate in the formation of the fibrillar-granular layer underneath the operculum. May function by regulating the expression of genes involved in pollen exine development. Kinase activity is required for its function in pollen development. KEYWORDS: ATP-binding;Cell membrane;Cytoplasm;Glycoprotein;Kinase;Lectin;Membrane;Nucleotide-binding;Phosphoprotein;Reference proteome;Serine/threonine-protein kinase;Signal;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein Cytoplasm, cytosol Note=During meiosis, localizes diffusely in the cytosol and plasma membrane of microspore mother cells (MMCs). During tetrad development, localizes at the corners. At late tetrad stage, accumulates to the four corners of the tetrad, assembled into ring-like structures marking future aperture sites. When microspores are released from tetrads and preliminary aperture structures has formed, remains in a distinctly ring-shaped distribution beneath the aperture in the plasma membrane between the annulus and operculum. " A0A0P0XII1,"PROTEIN NAMES: Chitin elicitor receptor kinase 1 (OsCERK1) (LysM domain receptor-like kinase 1) (LysM RLK1) (LysM-containing receptor-like kinase 1) (LysM domain receptor-like kinase 9) (OsLysM-RLK9) PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family ORGANISM: Oryza sativa subsp. japonica (Rice) SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. FUNCTION: Lysin motif (LysM) receptor kinase required as a cell surface receptor for chitin elicitor (chitooligosaccharides) signaling leading to innate immunity in response to biotic stresses. Involved in the resistance to pathogenic fungi, probably by sensing microbe-associated molecular patterns (MAMP) and pathogen-associated molecular patterns (PAMP). Involved in the detection of microbial peptidoglycans (PGNs) and mediates PGN response. Plays dual roles in PGN and chitin signaling during innate immunity. Acts as an adapter for LYP4 and LYP6 and mediates signal transduction from the extracellular to intracellular spaces. Participates in the activation of defense genes during response to PGN and chitin. Phosphorylates the downstream partner RLCK185 in response to chitin elicitation. KEYWORDS: 3D-structure;ATP-binding;Cell membrane;Chitin-binding;Disulfide bond;Glycoprotein;Immunity;Innate immunity;Kinase;Membrane;Nucleotide-binding;Plant defense;Receptor;Reference proteome;Serine/threonine-protein kinase;Signal;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass membrane protein " A0A0R4IKJ1,"PROTEIN NAMES: mRNA (2'-O-methyladenosine-N(6)-)-methyltransferase (Cap-specific adenosine methyltransferase) (CAPAM) (zCAPAM) (Phosphorylated CTD-interacting factor 1) PROTEIN FAMILY: CAPAM family ORGANISM: Danio rerio (Zebrafish) (Brachydanio rerio) SIMILARITY: Belongs to the CAPAM family. FUNCTION: Cap-specific adenosine methyltransferase that catalyzes formation of N(6),2'-O-dimethyladenosine cap (m6A(m)) by methylating the adenosine at the second transcribed position of capped mRNAs. KEYWORDS: 3D-structure;Methyltransferase;Nucleus;Reference proteome;Transferase SUBCELLULAR LOCATION: Nucleus " A0A126GUP6,"PROTEIN NAMES: Melanization protease 1 PROTEIN FAMILY: Peptidase S1 family, CLIP subfamily ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the peptidase S1 family. CLIP subfamily. FUNCTION: Serine protease which plays an essential role in the melanization immune response by acting downstream of sp7 to activate prophenoloxidase (PPO1). May function in diverse Hayan-dependent PPO1-activating cascades that are negatively controlled by different serpin proteins; Spn27A in the hemolymph and Spn77BA in the trachea. Regulation of melanization and PPO1 activation appears to be largely independent of the Toll signaling pathway. KEYWORDS: Alternative splicing;Calcium;Disulfide bond;Glycoprotein;Hydrolase;Metal-binding;Protease;Reference proteome;Serine protease;Signal;Zymogen " B7QK46,"PROTEIN NAMES: Glutaminyl-peptide cyclotransferase (Glutaminyl cyclase) (QC) (Glutaminyl-tRNA cyclotransferase) PROTEIN FAMILY: Glutaminyl-peptide cyclotransferase family ORGANISM: Ixodes scapularis (Black-legged tick) (Deer tick) SIMILARITY: Belongs to the glutaminyl-peptide cyclotransferase family. FUNCTION: Responsible for the biosynthesis of pyroglutamyl peptides. Seems to have a preference for substrates with neutral or hydrophobic amino-acid residues at the second and third positions. Shows activity towards the peptides [Gln-1]-corazonin, [Gln-1]-periviscerokinin and [Gln-1]-sulfakinin. KEYWORDS: 3D-structure;Acyltransferase;Disulfide bond;Glycoprotein;Metal-binding;Reference proteome;Secreted;Signal;Transferase;Zinc SUBCELLULAR LOCATION: Secreted " B7TB45,"PROTEIN NAMES: Neurotrophin 1 (Neurotrophic factor 1) (Protein spaetzle 2) (Protein spatzle 2) ORGANISM: Drosophila melanogaster (Fruit fly) FUNCTION: Neurotrophin which may function as a ligand for the Toll-related receptors Toll-7 and Tollo. Binds to Toll-7 and probably acts as its ligand in promoting motor axon targeting and neuronal survival in the central nervous system (CNS). Involved in synaptic targeting of ISNb/d motorneurons and also some SNa motorneurons. In larvae, involved in the negative regulation of the tracheal immune response to bacterial infection perhaps by acting as a ligand for the Toll-related receptor Tollo. May be involved in the normal development of specific neurons at the neuromuscular junction. KEYWORDS: Alternative splicing;Disulfide bond;Glycoprotein;Reference proteome;Signal MISCELLANEOUS: 'Spaetzle' means 'noodles' in German." B7U179,"PROTEIN NAMES: ARMADILLO BTB ARABIDOPSIS PROTEIN 1 (ABAP1) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: May act as a substrate-specific adapter of an E3 ubiquitin-protein ligase complex (CUL3-RBX1-BTB) which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (By similarity). In association with TCP24, exerts a negative role in cell proliferation in leaves, possibly by inhibiting mitotic DNA replication. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Nucleus;Reference proteome;Repeat;Ubl conjugation pathway SUBCELLULAR LOCATION: Nucleus " B7ZWR6,"PROTEIN NAMES: Outer envelope protein 61 (Tetratricopeptide repeat domain-containing protein 7) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Plays a role in protein import into the endoplasmic reticulum (ER). May function as chaperone docking protein during post-translational protein translocation into the ER. Chaperone receptor mediating Hsp70-dependent protein targeting to chloroplasts. Interacts specifically with some chloroplast precursors, but not with mitochondrial precursors. Able to select precursors for delivery to the chloroplast translocase independently of Hsp70. KEYWORDS: Chloroplast;Endoplasmic reticulum;Membrane;Plastid;Plastid outer membrane;Protein transport;Reference proteome;Repeat;TPR repeat;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Single-pass membrane protein Plastid, chloroplast outer membrane ; Single-pass membrane protein Note=Resides most likely exclusively in the ER membrane. " B8A4F0,PROTEIN NAMES: Palmitoyltransferase ZDHHC16A (Zinc finger DHHC domain-containing protein 16A) (DHHC-16A) PROTEIN FAMILY: DHHC palmitoyltransferase family ORGANISM: Danio rerio (Zebrafish) (Brachydanio rerio) SIMILARITY: Belongs to the DHHC palmitoyltransferase family. FUNCTION: Palmitoyl acyltransferase that mediates palmitoylation of proteins and is required during embryonic heart development. Involved in the proliferation of neural stem cells by regulating the FGF/ERK pathway (By similarity). Involved in the proliferation of neural stem cells by regulating the FGF/ERK pathway. KEYWORDS: Acyltransferase;Endoplasmic reticulum;Membrane;Reference proteome;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein B8AL97,"PROTEIN NAMES: Cupincin (52 kDa globulin-like protein) (allergen Ory s NRA) PROTEIN FAMILY: 7S seed storage protein family ORGANISM: Oryza sativa subsp. indica (Rice) SIMILARITY: Belongs to the 7S seed storage protein family. FUNCTION: Seed storage protein (Probable). Globulin-like protein that acts as a zinc metalloprotease. Cleaves specifically between Leu-15 and Tyr-16 of insulin B chain, and Gln-1 and Leu-2 of neurotensin (NT) peptide in vitro. May play a role as an initiating endopeptidase in germinating seeds. KEYWORDS: Allergen;Direct protein sequencing;Glycoprotein;Hydrolase;Metal-binding;Metalloprotease;Protease;Reference proteome;Secreted;Seed storage protein;Signal;Storage protein;Zinc SUBCELLULAR LOCATION: Secreted " B8B183,"PROTEIN NAMES: Protein ABERRANT PANICLE ORGANIZATION 1 (F-box protein 321) (OsFbox321) (Protein STRONG CULM 2) ORGANISM: Oryza sativa subsp. indica (Rice) FUNCTION: Component of SCF(ASK-cullin-F-box) E3 ubiquitin ligase complexes, which may mediate the ubiquitination and subsequent proteasomal degradation of target proteins (By similarity). Together with FL/APO2, involved in the temporal regulation of meristem identity during both vegetative and reproductive developments in an APO2-dependent manner (By similarity). Promotes spikelet formation by suppressing the precocious conversion of inflorescence meristems to spikelet meristems, probably via a positive regulation of class-C floral homeotic genes, but not of class-B genes, and through the control of cell proliferation in meristems. Mediates culm development and strength/diameter enhancement at internodes. Required for the regulation of the plastochron, floral organ identity, and floral determinacy (By similarity). Controls the number of primary rachis branches (PRBs). May trigger the formation of vascular bundle systems which, consequently, promote carbohydrate translocation to panicles. Involved in ozone-induced grain yield regulation. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Activator;Developmental protein;Differentiation;Flowering;Kelch repeat;Membrane;Reference proteome;Repeat;Transmembrane;Transmembrane helix;Ubl conjugation pathway SUBCELLULAR LOCATION: Membrane ; Multi-pass membrane protein MISCELLANEOUS: Ozone has various impact on different cultivars; O.sativa subsp. japonica cv. Sasanishiki exhibits ozone-induced leaf injury, but no grain yield loss, and, by contrast, O.sativa subsp. indica cv. Habataki has grain yield loss with minimal leaf injury upon ozone treatment.; MISCELLANEOUS: Plants harboring the PBN6 quantitative trait locus (QTL) (e.g. HI1 allele in O.sativa subsp. indica cv. Habataki) exhibit bigger peduncle diameter due to larger vascular bundles and an increased number of primary rachis branches (PRBs) and of the number of grains per panicle, thus leading to increased grain yield.; MISCELLANEOUS: Plants harboring the SCM2 quantitative trait locus (QTL) exhibit both an enhancement of culm strength at internodes and an increased spikelet number leading to higher crop productivity and better grain yield." B8PYG1,"PROTEIN NAMES: NMDA receptor synaptonuclear signaling and neuronal migration factor (Nasal embryonic luteinizing hormone-releasing hormone factor) (Nasal embryonic LHRH factor) PROTEIN FAMILY: NSMF family ORGANISM: Danio rerio (Zebrafish) (Brachydanio rerio) SIMILARITY: Belongs to the NSMF family. FUNCTION: Stimulates outgrowth of olfactory axons and migration of hypophysiotropic gonadotropin-releasing hormone 3 (GnRH3) neurons. May couple NMDA-sensitive glutamate receptor signaling to the nucleus and trigger long-lasting changes in the cytoarchitecture of dendrites and spine synapse processes. KEYWORDS: Cell membrane;Cell projection;Cytoplasm;Cytoskeleton;Lipoprotein;Membrane;Myristate;Nucleus;Reference proteome;Synapse;Synaptosome SUBCELLULAR LOCATION: Nucleus Nucleus envelope Nucleus membrane Nucleus matrix Cytoplasm Cytoplasm, cell cortex Cytoplasm, cytoskeleton Cell membrane ; Peripheral membrane protein Cell projection, dendrite Synapse Synapse, synaptosome Postsynaptic density Membrane " B8QHP1,"PROTEIN NAMES: Cytochrome P450 52-M1 (CYP52-M1) (Cytochrome P450 monooxygenase CYP52-M1) PROTEIN FAMILY: Cytochrome P450 family ORGANISM: Starmerella bombicola (Yeast) (Candida bombicola) SIMILARITY: Belongs to the cytochrome P450 family. FUNCTION: Catalyzes the first step of sophorolipid biosynthesis. Catalyzes the terminal (at the omega-position) or subterminal (at the omega(-1)-position) hydroxylation of a fatty acid. This converts the fatty acid to a substrate for the subsequent glycosyltransferase reactions. Oleic acid is the preferred substrate, but it acts on various other C-16, C-18 and C-20 saturated and unsaturated fatty acids, namely palmitic, palmitoleic, stearic, linoleic, cis-9,10-epoxystearic, trans-9,10-epoxystearic and arachidonic acid. KEYWORDS: Heme;Iron;Membrane;Metal-binding;Monooxygenase;Oxidoreductase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Single-pass membrane protein " B8XY56,"PROTEIN NAMES: Ribonuclease T2 (RNase Dre2) PROTEIN FAMILY: RNase T2 family ORGANISM: Danio rerio (Zebrafish) (Brachydanio rerio) SIMILARITY: Belongs to the RNase T2 family. FUNCTION: Has ribonuclease activity, with higher activity at acidic pH. Probably is involved in lysosomal degradation of ribosomal RNA. KEYWORDS: Disulfide bond;Endonuclease;Endoplasmic reticulum;Glycoprotein;Hydrolase;Lyase;Lysosome;Nuclease;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Lysosome lumen Endoplasmic reticulum lumen Secreted " B8YG19,"PROTEIN NAMES: Bifunctional acetylxylan esterase/xylanase XynS20E [Includes: Acetylxylan esterase ; Endo-1,4-beta-xylanase (Xylanase) (1,4-beta-D-xylan xylanohydrolase)] PROTEIN FAMILY: AxeA family; Glycosyl hydrolase 11 (cellulase G) family ORGANISM: Neocallimastix patriciarum (Rumen fungus) SIMILARITY: In the N-terminal section; belongs to the axeA family.; SIMILARITY: In the C-terminal section; belongs to the glycosyl hydrolase 11 (cellulase G) family. FUNCTION: Bifunctional acetylxylan esterase/xylanase involved in the hydrolysis of xylan, a major structural heterogeneous polysaccharide found in plant biomass representing the second most abundant polysaccharide in the biosphere, after cellulose. Degrades xylan from acetylxylan, beechwood, birchwood, and oat spelt, and releases acetate from 4-methylumbelliferyl acetate and beta-D-xylose tetraacetate. No activity is observed against carboxy methyl cellulose, beta-glucan, p-nitrophenol acetate, p-nitrophenol laurate, p-nitrophenol myristate, p-nitrophenol, palmitate, or beta-naphthol acetate. PATHWAY: Glycan degradation; xylan degradation. KEYWORDS: Carbohydrate metabolism;Glycoprotein;Glycosidase;Hydrolase;Polysaccharide degradation;Repeat;Secreted;Signal;Xylan degradation SUBCELLULAR LOCATION: Secreted " B9DFU2,"PROTEIN NAMES: Cytochrome P450 711A1 (Protein MORE AXILLARY BRANCHES 1) PROTEIN FAMILY: Cytochrome P450 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the cytochrome P450 family. FUNCTION: Involved in the biosynthesis of strigolactone natural products, bioactive compounds promoting plant fitness and soil microbe interactions, but preventing shoot branching. Converts carlactone to carlactonoic acid by catalyzing consecutive oxidations at C-19 to convert the C-19 methyl group into carboxylic acid. Prefers 11R-carlactone to 11S-carlactone as substrate. Acts downstream of CCD7/MAX3 and CCD8/MAX4 in strigolactone signaling pathway and may be implicated in synthesis of carotenoid-derived branch regulators. Acts as a positive regulator of the flavonoid pathway in the late vegetative stage plant. Strigolactones are hormones that inhibit tillering and shoot branching through the MAX-dependent pathway, contribute to the regulation of shoot architectural response to phosphate-limiting conditions and function as rhizosphere signal that stimulates hyphal branching of arbuscular mycorrhizal fungi and trigger seed germination of root parasitic weeds. KEYWORDS: Alternative splicing;Heme;Iron;Membrane;Metal-binding;Monooxygenase;Oxidoreductase;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Single-pass membrane protein MISCELLANEOUS: The branching phenotypes of the max1, ccd7/max3 and ccd8/max4 mutants can be rescued by exogenous treatment with the synthetic strigolactone analogs GR24 and 4BD (Probable). However, the max1 mutant does not respond to carlactone (CL) (Probable)." B9J8S0,"PROTEIN NAMES: Pterin deaminase (PDA) PROTEIN FAMILY: Metallo-dependent hydrolases superfamily, Pterin deaminase family ORGANISM: Rhizobium rhizogenes (strain K84 / ATCC BAA-868) (Agrobacterium radiobacter) SIMILARITY: Belongs to the metallo-dependent hydrolases superfamily. Pterin deaminase family. FUNCTION: Catalyzes the deamination of many pterin metabolites, such as formylpterin, pterin-6-carboxylate, pterin-7-carboxylate, pterin, hydroxymethylpterin, biopterin, D-(+)-neopterin, isoxanthopterin, sepiapterin, folate, xanthopterin, and 7,8-dihydrohydroxymethylpterin. May be involved in a degradative pathway for catabolizing pterin rings. KEYWORDS: Hydrolase;Metal-binding " B9TSP7,"PROTEIN NAMES: Fatty acyl-CoA reductase 6, chloroplastic (AtFAR6) PROTEIN FAMILY: Fatty acyl-CoA reductase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the fatty acyl-CoA reductase family. FUNCTION: Catalyzes the reduction of fatty acyl-CoA and -ACP (acyl carrier protein) substrates to fatty alcohols. Triggers the accumulation of C16 and, to a lower extent, of C18 fatty alcohols; converts palmitoyl-acyl carrier protein to the corresponding C16:0 alcohol with NAD(P)H as electron donor. Triggers also the formation of some C16:0 and C18:0 aldehydes. May be involved in the generation of C30 primary alcohol. KEYWORDS: Alternative splicing;Chloroplast;Lipid biosynthesis;Lipid metabolism;NADP;Oxidoreductase;Plastid;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast " C0HK25,"PROTEIN NAMES: Lectin ADEL ORGANISM: Aplysia dactylomela (Spotted sea hare) FUNCTION: Binds in decreasing order of affinity: galacturonic acid, D-galactosamine, methyl-alpha-D-galactopyranoside and further galactose-containing carbohydrates. Has hemagglutinating activity against human and rabbit erythrocytes. KEYWORDS: Direct protein sequencing;Disulfide bond;Glycoprotein;Hemagglutinin;Lectin " C0HK27,PROTEIN NAMES: Lectin (DlyL) PROTEIN FAMILY: Leguminous lectin family ORGANISM: Dioclea lasiophylla SIMILARITY: Belongs to the leguminous lectin family. FUNCTION: D-mannose-binding lectin that also binds alpha-methyl-D-mannoside with even higher affinity. Has hemagglutinating activity against rabbit erythrocytes. Shows toxicity against the brine shrimp A.nauplii. Induces reversible paw edema and hypernociceptivity in rats. KEYWORDS: 3D-structure;Calcium;Direct protein sequencing;Hemagglutinin;Lectin;Manganese;Mannose-binding;Metal-binding;Signal;Toxin MISCELLANEOUS: Binds one manganese (or another transition metal) ion and one calcium ion. The metal ions are essential for the saccharide-binding and cell-agglutinating activities. C0HKM3,"PROTEIN NAMES: Hyaluronidase conohyal-P1 (Hyaluronoglucosaminidase) PROTEIN FAMILY: Glycosyl hydrolase 56 family ORGANISM: Conus purpurascens (Purple cone) SIMILARITY: Belongs to the glycosyl hydrolase 56 family. FUNCTION: Hyaluronidase catalyzes the hydrolysis of hyaluronic acid (HA), an anionic, nonsulfated glycosaminoglycan distributed widely throughout connective, epithelial, and neural tissues. In venom, they are known to enhance diffusion of the venom by degrading the extracellular matrix (Probable). KEYWORDS: Direct protein sequencing;Disulfide bond;EGF-like domain;Glycoprotein;Glycosidase;Hydrolase;Secreted;Signal SUBCELLULAR LOCATION: Secreted " C0HLG3,"PROTEIN NAMES: Ribonuclease ageritin (Ribotoxin) PROTEIN FAMILY: Ribotoxin-like family ORGANISM: Cyclocybe aegerita (Black poplar mushroom) (Agrocybe aegerita) SIMILARITY: Belongs to the ribotoxin-like family. FUNCTION: Fungal ribonuclease involved in fungal defense. Highly specific and highly toxic fungal endonuclease that cleaves a single phosphodiester bond in the 28S RNA of eukaryotic ribosomes at a universally conserved GAGA tetraloop of the sarcin-ricin loop (SRL). The damage of the SRL inhibits the binding of translation elongation factors and halts protein biosynthesis, ultimately resulting in the death of the target cells. Shows antitumor activity. Exerts cytotoxicity and induces apoptosis towards rat glial cells and human glioma cells, and also displays some activity towards human neurolastoma cell lines. Shows a strong entomotoxicity against Aedes aegypti larvae, yet no nematotoxicity against nematodes. KEYWORDS: Direct protein sequencing;Glycoprotein;Hydrolase;Lyase;Magnesium;Metal-binding;Nuclease;Signal;Vacuole SUBCELLULAR LOCATION: Vacuole lumen Note=Possibly sequestered into the vacuole to avoid its toxic activity on ribosomes. " C0LGR6,"PROTEIN NAMES: Probable LRR receptor-like serine/threonine-protein kinase At4g29180 PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. KEYWORDS: Alternative splicing;ATP-binding;Glycoprotein;Kinase;Leucine-rich repeat;Membrane;Nucleotide-binding;Phosphoprotein;Receptor;Reference proteome;Repeat;Serine/threonine-protein kinase;Signal;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Single-pass type I membrane protein " C0LGR9,"PROTEIN NAMES: Probable LRR receptor-like serine/threonine-protein kinase At4g31250 PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. KEYWORDS: Alternative splicing;ATP-binding;Glycoprotein;Kinase;Leucine-rich repeat;Membrane;Nucleotide-binding;Phosphoprotein;Receptor;Reference proteome;Repeat;Serine/threonine-protein kinase;Signal;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Single-pass type I membrane protein " C0LGX3,"PROTEIN NAMES: LRR receptor-like serine/threonine-protein kinase HSL2 (Protein HAESA-LIKE2) PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. FUNCTION: Receptor-like serine/threonine-kinase acting on substrates that controls floral organ abscission. Regulated by the 'INFLORESCENCE DEFICIENT IN ABSCISSION' (IDA) family of ligands. KEYWORDS: ATP-binding;Cell membrane;Glycoprotein;Kinase;Leucine-rich repeat;Membrane;Nucleotide-binding;Phosphoprotein;Reference proteome;Repeat;Serine/threonine-protein kinase;Signal;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein " C0LT23,"PROTEIN NAMES: Ceramide kinase (OsCERK) ORGANISM: Oryza sativa subsp. japonica (Rice) FUNCTION: Catalyzes specifically the phosphorylation of ceramide to form ceramide 1-phosphate. Possesses activity on ceramide analog (C6 synthetic ceramide) in vitro. Ceramide is a critical sphingolipid metabolite that induces programmed cell death (PCD) in plants and ceramide-1-phosphate has a PCD suppressive effect. Thus, ceramide phosphorylation plays a role in the modulation of PCD and CERK activity is crucial for the maintenance of cell viability. KEYWORDS: ATP-binding;Calcium;Kinase;Magnesium;Metal-binding;Nucleotide-binding;Reference proteome;Transferase MISCELLANEOUS: Overexpression of CERK in the Arabidopsis mutant acd5 restores wild-type phenotype." C0QRQ2,"PROTEIN NAMES: Glucosyl-3-phosphoglycerate synthase (GpgS) PROTEIN FAMILY: Glycosyltransferase 2 family ORGANISM: Persephonella marina (strain DSM 14350 / EX-H1) SIMILARITY: Belongs to the glycosyltransferase 2 family. FUNCTION: Involved in the biosynthesis of 6-O-methylglucose lipopolysaccarides (MGLPs). Catalyzes the transfer of a glucose (Glc) moiety from uridine diphosphate (UDP-Glc) to the position 2 of 3-phospho-D-glycerate (3-PGA) to form glucosyl-3-phosphoglycerate (GPG). GpgS is most active with UDP-glucose, followed by GDP-glucose, ADP-glucose, and to a lesser extent, TDP-glucose. 3-PGA is the only acceptor for these glucosyl donors. KEYWORDS: Cobalt;Glycosyltransferase;Magnesium;Manganese;Metal-binding;Nickel;Reference proteome;Transferase " C0SPF7,"PROTEIN NAMES: Farnesoate epoxidase (Cytochrome P450 15C1) (Protein dimolting) (mod) PROTEIN FAMILY: Cytochrome P450 family ORGANISM: Bombyx mori (Silk moth) SIMILARITY: Belongs to the cytochrome P450 family. FUNCTION: Catalyzes the conversion of farnesoate to juvenile hormone III acid in juvenile hormone biosynthesis. KEYWORDS: Heme;Iron;Metal-binding;Monooxygenase;Oxidoreductase;Reference proteome;Signal MISCELLANEOUS: The enzyme is specifically found in lepidoptera (moths and butterflies) and is specific for farnesoate. Other insects contain the methyl farnesoate epoxidase, which is specific for methyl farnesoate." C5C7X8,"PROTEIN NAMES: DNA gyrase subunit B PROTEIN FAMILY: Type II topoisomerase GyrB family ORGANISM: Micrococcus luteus (strain ATCC 4698 / DSM 20030 / JCM 1464 / NBRC 3333 / NCIMB 9278 / NCTC 2665 / VKM Ac-2230) (Micrococcus lysodeikticus) SIMILARITY: Belongs to the type II topoisomerase GyrB family. FUNCTION: A type II topoisomerase that negatively supercoils DNA in an ATP-dependent manner. About 140 bp of DNA wraps around gyrase in the presence or absence of ATP, when ATP is added negative supercoils are made.; FUNCTION: A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner. KEYWORDS: ATP-binding;Cytoplasm;DNA-binding;Isomerase;Magnesium;Metal-binding;Nucleotide-binding;Reference proteome;Topoisomerase SUBCELLULAR LOCATION: Cytoplasm MISCELLANEOUS: Few gyrases are as efficient as E.coli at forming negative supercoils. Not all organisms have 2 type II topoisomerases; in organisms with a single type II topoisomerase this enzyme also has to decatenate newly replicated chromosomes." C5H8J1,"PROTEIN NAMES: Crossover junction endonuclease EME1B (Essential meiotic endonuclease 1B) (AtEME1B) PROTEIN FAMILY: EME1/MMS4 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the EME1/MMS4 family. FUNCTION: Interacts with MUS81 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, D-loops, replication forks, nicked Holliday junctions and also intact Holliday junctions with a reduced efficiency. May be required in mitosis for the processing of stalled or collapsed replication fork intermediates. Plays a role in DNA repair and in genotoxic stress-induced homologous recombination (HR) in somatic cells. Mediates a subset of meiotic recombination events that are insensitive to crossover interference. KEYWORDS: Calcium;Cell cycle;Cell division;Coiled coil;DNA damage;DNA recombination;DNA repair;Endonuclease;Hydrolase;Magnesium;Meiosis;Metal-binding;Mitosis;Nuclease;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus " C6KIE6,"PROTEIN NAMES: Extra-large guanine nucleotide-binding protein 2 (Extra-large GTP-binding protein 2) (Extra-large G-protein 2) PROTEIN FAMILY: G-alpha family, XLG subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the G-alpha family. XLG subfamily. FUNCTION: Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems (By similarity). Binds GTP with specificity. Plays a role in the root morphogenesis by regulation of the cell proliferation. Acts as a positive regulator in resistance to pathogen that triggers the salicylic acid (SA) pathway. Promotes the DNA binding activity of RTV1 specifically to promoter regions of FT and SOC1 in vivo leading to the activation of floral integrator genes. KEYWORDS: Calcium;GTP-binding;Metal-binding;Nucleotide-binding;Nucleus;Plant defense;Reference proteome;Transducer;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: Dark-grown xlg1-1 xlg2-1 xlg3-1 triple mutant plants showed markedly increased primary root length compared with wild-type plants. Dark-grown roots of the xlg triple mutants also showed altered sensitivity to sugars, abscisic acid (ABA) hyposensitivity and ethylene hypersensitivity, whereas seed germination in xlg triple mutants was hypersensitive to osmotic stress and ABA." C6KRL6,"PROTEIN NAMES: Zip homologous protein 3 (Zip3-homologous protein) ORGANISM: Caenorhabditis elegans FUNCTION: Recruited co-dependently with zhp-4 to the synaptonemal complex between homologous chromosome pairs to regulate the formation and number of crossover events between homologs during meiotic recombination. In the early stages of pachytene, in complex with zhp-4, recruited by the zhp-1-zhp-2 heterodimer to designated crossover sites along the homolog pair to stabilize other pro-crossover factors such as rmh-1, msh-5 and cosa-1. This in turn facilitates crossover and promotes the formation of chiasma in each meiotic nucleus at the late pachytene stage of meiosis. Plays a role in the segregation of homologous chromosomes following the completion of crossovers. Together with him-14 and msh-5 plays a role in the activation of DNA damage-dependent apoptosis at the DNA damage checkpoint in pachytene cells. KEYWORDS: Alternative splicing;Chromosome;Coiled coil;DNA recombination;Meiosis;Metal-binding;Reference proteome;Zinc;Zinc-finger SUBCELLULAR LOCATION: Chromosome Note=Co-localizes with zhp-4 to chromosomes from mitosis to early diakinesis in the germline. Co-localizes with syp-1, a component of the synaptonemal complex, throughout the gonad from early prophase to mid-pachytene. In early pachytene, co-localizes with syp-1 as puncta along chromosomes. In pachytene nuclei, localizes in linear arrays in the space in between synapsed chromosomes. Does not localize to unsynapsed chromosomes. From mid-pachytene, co-localizes with cosa-1 at crossover sites of recombination intermediates, and gradually disassociates from syp-1 along both chromosome arms. Co-localizes with brc-1 at crossover sites in mid-late pachytene nuclei. At late pachytene, localizes asymmetrically on synapsed chromosomes. At late pachytene and early diplotene localizes to a single focus at the boundary between the long and short arm of each pair of homologous chromosomes. At late pachytene, localization at chromosomes is not dependent on syp-1. In diakinesis, does not localize to chromosomes, but is dispersed between chromosomes. " C6KRN1,PROTEIN NAMES: Suppressor of aph-1 ORGANISM: Caenorhabditis elegans FUNCTION: Involved in negative regulation of early and late embryonic Notch signaling. KEYWORDS: 3D-structure;Alternative splicing;Developmental protein;Notch signaling pathway;Reference proteome C7AJA4,"PROTEIN NAMES: Terminal uridylyltransferase 7 (TUTase 7) (Mitochondrial editosome-like complex associated TUTase) (TbMEAT1) PROTEIN FAMILY: DNA polymerase type-B-like family ORGANISM: Trypanosoma brucei brucei SIMILARITY: Belongs to the DNA polymerase type-B-like family. FUNCTION: Terminal uridylyltransferase which, as part of the mitochondrial RNA editing core-like complex (RECC-like), is involved in the post-transcriptional editing of mitochondrial RNA, a process involving the addition and deletion of uridine (U) nucleotides in the pre-mRNA. Specifically, catalyzes the addition of U to single-stranded RNA with a preference for a 3'-terminal U and adds the number of Us specified by a guide RNA (gRNA) to precleaved double-stranded RNA editing substrates. Essential for insect and bloodstream developmental forms viability. KEYWORDS: 3D-structure;Magnesium;Metal-binding;Mitochondrion;mRNA processing;Nucleotide-binding;Nucleotidyltransferase;Transferase;Transit peptide SUBCELLULAR LOCATION: Mitochondrion matrix " C8VJW0,PROTEIN NAMES: Nicotinate catabolism cluster-specific transcription factor ORGANISM: Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) FUNCTION: Transcription factor that specifically regulates the expression of the hxn gene cluster that mediates the degradation of nicotinate and related metabolites. KEYWORDS: Metal-binding;Nucleus;Reference proteome;Repeat;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus D0E8I5,"PROTEIN NAMES: 2-amino-1-hydroxyethylphosphonate dioxygenase (glycine-forming) (Di-iron oxygenase) (Nonheme iron-dependent oxygenase) ORGANISM: Uncultured bacterium HF130_AEPn_1 FUNCTION: Involved in the degradation of the organophosphonate 2-aminoethylphosphonic acid (2-AEP) (Probable). Catalyzes the cleavage of the carbon-phosphorus bond of (2-amino-1-hydroxyethyl)phosphonic acid to yield glycine and phosphate through an oxidative mechanism. It reacts stereospecifically with the R-enantiomer of (2-amino-1-hydroxyethyl)phosphonic acid and is also able to use (R,R)-2-amino-1-hydroxypropylphosphonate as substrate. KEYWORDS: 3D-structure;Hydrolase;Iron;Metal-binding;Oxidoreductase " D0VWY5,PROTEIN NAMES: Glutathione amide reductase (GAR) PROTEIN FAMILY: Class-I pyridine nucleotide-disulfide oxidoreductase family ORGANISM: Marichromatium gracile (Chromatium gracile) SIMILARITY: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family. FUNCTION: Catalyzes the reduction of glutathione amide disulfide (GASSAG) to restore glutathione amide (GASH) in the presence of NADH. May play a role in GASH metabolism under anaerobic conditions as a sulfide carrier necessary for cytoplasmic sulfide oxidation. KEYWORDS: 3D-structure;Direct protein sequencing;Disulfide bond;FAD;Flavoprotein;Metal-binding;NAD;Nickel;Nucleotide-binding;Oxidoreductase;Redox-active center MISCELLANEOUS: The active site is a redox-active disulfide bond. D2CVN6,"PROTEIN NAMES: Telomeric repeat-binding subunit 1 (Telomerase-associated protein of 82 kDa) (p82) PROTEIN FAMILY: Replication factor A protein 1 family ORGANISM: Tetrahymena thermophila (strain SB210) SIMILARITY: Belongs to the replication factor A protein 1 family. FUNCTION: Single-stranded DNA (ssDNA)-binding protein that mediates the recruitment of telomerase to telomeric DNA. Telomerase is an essential ribonucleoprotein (RNP) enzyme that copies new telomeric repeats onto chromosome ends by repetitively synthesizing the short telomere-repeat sequence 5'-TTGGGG-3' using an RNA template component TER. Acts as a part of a replication protein A (RPA)-related subcomplex of the holoenzyme telomerase ribonucleoprotein complex: TEB1 specifically recognizes and binds telomeric ssDNA, thereby mediating the recruitment of the holoenzyme telomerase RNP complex to telomeres. TEB1 is related to RPA1 subunit of the RPA complex but is specific to telomeric DNA, which is not the case of RPA1. KEYWORDS: 3D-structure;Chromosome;DNA-binding;Metal-binding;Reference proteome;Telomere;Zinc;Zinc-finger SUBCELLULAR LOCATION: Chromosome, telomere " D2Z030,"PROTEIN NAMES: Cycloserine biosynthesis protein DcsG ORGANISM: Streptomyces lavendulae FUNCTION: Involved in the biosynthesis of the antibiotic D-cycloserine (DCS), a cyclic structural analog of D-alanine, used as an antitubercular agent. Catalyzes the synthesis of D-cycloserine from O-ureido-D-serine (D-OUS). It reacts with D-OUS, D-homocysteine and beta-aminooxy-D-alanine. KEYWORDS: 3D-structure;Antibiotic biosynthesis;ATP-binding;Ligase;Magnesium;Manganese;Metal-binding;Nucleotide-binding " D3J162,"PROTEIN NAMES: Protein VAPYRIN (MtVpy) (Protein HERMES) ORGANISM: Medicago truncatula (Barrel medic) (Medicago tribuloides) FUNCTION: Required for arbuscular mycorrhizal (AM) symbiosis with AM fungi (e.g. Glomus versiforme and Gigaspora gigantea) both during fungal passage across root epidermis and for arbuscule formation in cortical cells; this symbiosis promotes phosphorus (P) and copper (Cu) uptake. Essential for infection by symbiotic nitrogen-fixing rhizobial bacteria (e.g. Sinorhizobium meliloti) leading to the formation of root nodules. KEYWORDS: ANK repeat;Cell membrane;Cytoplasm;Membrane;Nucleus;Reference proteome;Repeat SUBCELLULAR LOCATION: Cytoplasm Nucleus Cell membrane ; Peripheral membrane protein ; Cytoplasmic side Note=In cells containing arbuscular mycorrhizal (AM) fungal hyphae and arbuscules, accumulates in small puncta that move through the cytoplasm, likely mobile spherical structures that are associated with the tonoplast referred to as 'tonospheres'. Present in cytoplasmic strands below hyphopodia. Observed associated with EX70I in zones adjacent to the periarbuscular membrane (PAM) around the arbuscule hyphal tips. Occasionally observed in the nucleus of cells containing fungal hyphae. " D3YN49,PROTEIN NAMES: Geminin coiled-coil domain-containing protein 1 (xGEMC1) PROTEIN FAMILY: GEMC1 family ORGANISM: Xenopus laevis (African clawed frog) SIMILARITY: Belongs to the GEMC1 family. FUNCTION: Regulator of DNA replication. Promotes initiation of chromosomal DNA replication by mediating topbp1- and cdk2-dependent recruitment of cdc45l onto replication origins. KEYWORDS: Cell cycle;Coiled coil;DNA replication;Nucleus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Nucleus Note=Associates with chromatin during pre-replication complex (pre-RC) formation following interaction with topbp1. D3Z291,"PROTEIN NAMES: Calcium homeostasis modulator protein 1 PROTEIN FAMILY: CALHM family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the CALHM family. FUNCTION: Pore-forming subunit of gustatory voltage-gated ion channels required for sensory perception of sweet, bitter and umami tastes. With CALHM3 forms a fast-activating voltage-gated ATP-release channel in type II taste bud cells, ATP acting as a neurotransmitter to activate afferent neural gustatory pathways. Acts both as a voltage-gated and calcium-activated ion channel: mediates neuronal excitability in response to membrane depolarization and low extracellular Ca(2+) concentration. Has poor ion selectivity and forms a wide pore (around 14 Angstroms) that mediates permeation of small ions including Ca(2+), Na(+), K(+) and Cl(-), as well as larger ions such as ATP(4-). Mediates Ca(2+) influx and downstream activation of the ERK1 and ERK2 cascade in neurons (By similarity). Triggers endoplasmic reticulum stress by reducing the calcium content of the endoplasmic reticulum (By similarity). May indirectly control amyloid precursor protein (APP) proteolysis and aggregated amyloid-beta (Abeta) peptides levels in a Ca(2+) dependent manner (By similarity). KEYWORDS: Calcium;Calcium channel;Calcium transport;Cell membrane;Disulfide bond;Endoplasmic reticulum;Glycoprotein;Ion channel;Ion transport;Lipoprotein;Membrane;Palmitate;Reference proteome;Sensory transduction;Taste;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Endoplasmic reticulum membrane ; Multi-pass membrane protein Basolateral cell membrane ; Multi-pass membrane protein. Note=Localizes to the basolateral membrane of epithelial cells including taste cells. Colocalizes with HSPA5 at the endoplasmic reticulum (By similarity). " D3Z902,"PROTEIN NAMES: F-box/WD repeat-containing protein 7 ORGANISM: Rattus norvegicus (Rat) FUNCTION: Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (By similarity). Recognizes and binds phosphorylated sites/phosphodegrons within target proteins and thereafter brings them to the SCF complex for ubiquitination (By similarity). Identified substrates include cyclin-E (CCNE1 or CCNE2), JUN, MYC, NOTCH1 released notch intracellular domain (NICD), NOTCH2, MCL1, MLST8, RICTOR and probably PSEN1 (By similarity). Acts as a negative regulator of JNK signaling by binding to phosphorylated JUN and promoting its ubiquitination and subsequent degradation (By similarity). SCF(FBXW7) complex mediates the ubiquitination and subsequent degradation of NFE2L1 (By similarity). Involved in bone homeostasis and negative regulation of osteoclast differentiation (By similarity). Regulates the amplitude of the cyclic expression of hepatic core clock genes and genes involved in lipid and glucose metabolism via ubiquitination and proteasomal degradation of their transcriptional repressor NR1D1; CDK1-dependent phosphorylation of NR1D1 is necessary for SCF(FBXW7)-mediated ubiquitination (By similarity). Also able to promote 'Lys-63'-linked ubiquitination in response to DNA damage (By similarity). The SCF(FBXW7) complex facilitates double-strand break repair following phosphorylation by ATM: phosphorylation promotes localization to sites of double-strand breaks and 'Lys-63'-linked ubiquitination of phosphorylated XRCC4, enhancing DNA non-homologous end joining (By similarity). PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Biological rhythms;Chromosome;Coiled coil;DNA damage;DNA repair;Nucleus;Phosphoprotein;Reference proteome;Repeat;Ubl conjugation;Ubl conjugation pathway;WD repeat SUBCELLULAR LOCATION: Nucleus, nucleoplasm Chromosome Note=Localizes to site of double-strand breaks following phosphorylation by ATM. " D3ZEH5,"PROTEIN NAMES: SID1 transmembrane family member 2 PROTEIN FAMILY: SID1 family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the SID1 family. FUNCTION: Mediates the translocation of RNA and DNA across the lysosomal membrane during RNA and DNA autophagy (RDA), a process in which RNA or DNA is directly imported into lysosomes in an ATP-dependent manner, and degraded. Involved in the uptake of single-stranded oligonucleotides by living cells, a process called gymnosis (By similarity). In vitro, mediates the uptake of linear DNA more efficiently than that of circular DNA, but exhibits similar uptake efficacy toward RNA and DNA. Binds long double-stranded RNA (dsRNA) (500 - 700 base pairs), but not dsRNA shorter than 100 bp (By similarity). KEYWORDS: Cell membrane;DNA-binding;Glycoprotein;Lysosome;Membrane;Phosphoprotein;Reference proteome;RNA-binding;Signal;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Lysosome membrane ; Multi-pass membrane protein Cell membrane Note=Mainly localizes to lysosomes and only partly to the plasma membrane (By similarity). Lysosomal localization is required for SIDT2-mediated intracellular degradation of endogenous RNA (By similarity). " D3ZGQ5,"PROTEIN NAMES: Serine/threonine-protein kinase Nek8 (Never in mitosis A-related kinase 8) (NimA-related protein kinase 8) PROTEIN FAMILY: Protein kinase superfamily, NEK Ser/Thr protein kinase family, NIMA subfamily ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily. FUNCTION: Required for renal tubular integrity. May regulate local cytoskeletal structure in kidney tubule epithelial cells. May regulate ciliary biogenesis through targeting of proteins to the cilia. Plays a role in organogenesis and is involved in the regulation of the Hippo signaling pathway (By similarity). KEYWORDS: ATP-binding;Cell projection;Cilium;Cytoplasm;Cytoskeleton;Disease variant;Kinase;Magnesium;Metal-binding;Nucleotide-binding;Phosphoprotein;Reference proteome;Repeat;Serine/threonine-protein kinase;Transferase SUBCELLULAR LOCATION: Cytoplasm Cytoplasm, cytoskeleton Cell projection, cilium Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Note=Predominantly cytoplasmic. Localizes to the proximal region of the primary cilium and is not observed in dividing cells (By similarity). " D3ZHH1,"PROTEIN NAMES: Delta-like protein 4 (Drosophila Delta homolog 4) (Delta4) ORGANISM: Rattus norvegicus (Rat) FUNCTION: Involved in the Notch signaling pathway as Notch ligand. Activates NOTCH1 and NOTCH4. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. Essential for retinal progenitor proliferation. Required for suppressing rod fates in late retinal progenitors as well as for proper generation of other retinal cell types. During spinal cord neurogenesis, inhibits V2a interneuron fate (By similarity). KEYWORDS: 3D-structure;Cell membrane;Developmental protein;Disulfide bond;EGF-like domain;Glycoprotein;Membrane;Reference proteome;Repeat;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein " D3ZHP7,"PROTEIN NAMES: Serine/threonine-protein kinase ULK3 (Unc-51-like kinase 3) PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family, APG1/unc-51/ULK1 subfamily ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily. FUNCTION: Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. Acts as a negative regulator of SHH signaling in the absence of SHH ligand: interacts with SUFU, thereby inactivating the protein kinase activity and preventing phosphorylation of GLI proteins (GLI1, GLI2 and/or GLI3). Positively regulates SHH signaling in the presence of SHH: dissociates from SUFU, autophosphorylates and mediates phosphorylation of GLI2, activating it and promoting its nuclear translocation. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently. Also acts as a regulator of autophagy: following cellular senescence, able to induce autophagy (By similarity). KEYWORDS: ATP-binding;Autophagy;Cytoplasm;Kinase;Nucleotide-binding;Phosphoprotein;Reference proteome;Repeat;Serine/threonine-protein kinase;Transferase SUBCELLULAR LOCATION: Cytoplasm Note=Localizes to pre-autophagosomal structure during cellular senescence. " D3ZQF4,"PROTEIN NAMES: Inactive peptidyl-prolyl cis-trans isomerase FKBP6 (Inactive PPIase FKBP6) (36 kDa FK506-binding protein) (FK506-binding protein 6) (Immunophilin FKBP36) PROTEIN FAMILY: FKBP6 family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the FKBP6 family. FUNCTION: Co-chaperone required during spermatogenesis to repress transposable elements and prevent their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Acts as a co-chaperone via its interaction with HSP90 and is required for the piRNA amplification process, the secondary piRNA biogenesis. May be required together with HSP90 in removal of 16 nucleotide ping-pong by-products from Piwi complexes, possibly facilitating turnover of Piwi complexes (By similarity). KEYWORDS: Chromosome;Cytoplasm;Differentiation;Meiosis;Nucleus;Reference proteome;Repeat;RNA-mediated gene silencing;Spermatogenesis;TPR repeat SUBCELLULAR LOCATION: Cytoplasm, cytosol Nucleus Chromosome Note=Does not localize to pi-bodies. Localizes to meiotic chromosome cores and regions of homologous chromosome synapsis (By similarity). " D4A4K3,"PROTEIN NAMES: Beclin 1-associated autophagy-related key regulator (Barkor) (Autophagy-related protein 14-like protein) (Atg14L) PROTEIN FAMILY: ATG14 family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the ATG14 family. FUNCTION: Required for both basal and inducible autophagy. Determines the localization of the autophagy-specific PI3-kinase complex. Plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine. Promotes BECN1 translocation from the trans-Golgi network to autophagosomes. Enhances PIK3C3 activity in a BECN1-dependent manner. Essential for the autophagy-dependent phosphorylation of BECN1. Stimulates the phosphorylation of BECN1, but suppresses the phosphorylation PIK3C3 by AMPK. Binds to STX17-SNAP29 binary t-SNARE complex on autophagosomes and primes it for VAMP8 interaction to promote autophagosome-endolysosome fusion. Modulates the hepatic lipid metabolism (By similarity). KEYWORDS: Autophagy;Coiled coil;Cytoplasm;Endoplasmic reticulum;Membrane;Phosphoprotein;Reference proteome;Ubl conjugation SUBCELLULAR LOCATION: Cytoplasm Endoplasmic reticulum membrane ; Peripheral membrane protein Preautophagosomal structure membrane ; Peripheral membrane protein Note=Cytosolic under nutrient-rich conditions (By similarity). Following autophagy stimuli, such as starvation or rapamycin induction, predominantly detected in cytoplasmic foci, identified as isolation membranes and autophagosomes (By similarity). Accumulates on highly curved PtdIns(3)P enriched autophagic membrane via its BATS domain to sense and maintain membrane curvature (By similarity). Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme (By similarity). " D4GSE6,PROTEIN NAMES: 2-dehydro-3-deoxygluconokinase/2-dehydro-3-deoxygalactonokinase (2-dehydro-3-deoxyglucono/galactono-kinase) (2-keto-3-deoxygluconate/2-keto-3-deoxygalactonate kinase) (KDG kinase) (KDGK-1) PROTEIN FAMILY: Carbohydrate kinase PfkB family ORGANISM: Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii) SIMILARITY: Belongs to the carbohydrate kinase PfkB family. FUNCTION: Involved in the degradation of glucose via the semi-phosphorylative Entner-Doudoroff pathway. Catalyzes the phosphorylation of 2-keto-3-deoxygluconate (KDG) to produce 2-keto-3-deoxy-6-phosphogluconate (KDPG). Also catalyzes efficiently the phosphorylation of 2-keto-3-deoxygalactonate (KDGal) to 2-keto-3-deoxy-6-phosphogalactonate (KDPGal). PATHWAY: Carbohydrate acid metabolism; 2-dehydro-3-deoxy-D-gluconate degradation; D-glyceraldehyde 3-phosphate and pyruvate from 2-dehydro-3-deoxy-D-gluconate: step 1/2. KEYWORDS: ATP-binding;Carbohydrate metabolism;Kinase;Nucleotide-binding;Reference proteome;Transferase D4GSH7,PROTEIN NAMES: Cell division protein FtsZ 2 PROTEIN FAMILY: FtsZ family ORGANISM: Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii) SIMILARITY: Belongs to the FtsZ family. FUNCTION: Essential cell division protein that forms a contractile ring structure (Z ring) at the future cell division site. The regulation of the ring assembly controls the timing and the location of cell division. One of the functions of the FtsZ ring is to recruit other cell division proteins to the septum to produce a new cell wall between the dividing cells. Binds GTP and shows GTPase activity (By similarity). Required for division ring constriction. KEYWORDS: Cell cycle;Cell division;Cytoplasm;GTP-binding;Nucleotide-binding;Reference proteome;Septation SUBCELLULAR LOCATION: Cytoplasm Note=Assembles at midcell at the inner surface of the cytoplasmic membrane. D4GTS4,"PROTEIN NAMES: Desampylase (HvJAMM1) PROTEIN FAMILY: Peptidase M67B family ORGANISM: Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii) SIMILARITY: Belongs to the peptidase M67B family. FUNCTION: Metalloprotease that displays desampylase (DSAMP) activity, cleaving ubiquitin-like small archaeal modifier proteins (SAMP1, SAMP2 and SAMP3) from protein conjugates (isopeptide- and linear-linked). Thus, likely regulates sampylation and the pools of 'free' SAMP available for protein modification. Functions as a specific and not a general protease since it is unable to hydrolyze a variety of unmodified proteins otherwise hydrolyzed by proteinase K. KEYWORDS: Hydrolase;Metal-binding;Metalloprotease;Protease;Reference proteome;Zinc MISCELLANEOUS: Is optimally active at NaCl concentrations of 0.7-2 M, and displays little to no activity at low concentrations of salt (150 mM NaCl)." D4GYG6,PROTEIN NAMES: Archaeal glycosylation protein Q ORGANISM: Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii) FUNCTION: Putative isomerase involved in the N-glycosylation pathway. Required for the appearance of the methyl ester of hexuronic acid found at position four of the pentasaccharide N-linked to the S-layer glycoprotein. Either involved in preparing the third sugar for attachment of the fourth pentasaccharide subunit or processing the fourth sugar prior to its addition to the lipid-linked trisaccharide. PATHWAY: Cell surface structure biogenesis; S-layer biogenesis. KEYWORDS: Cytoplasm;Reference proteome SUBCELLULAR LOCATION: Cytoplasm D4N500,"PROTEIN NAMES: Thebaine 6-O-demethylase (Canadine demethylase) PROTEIN FAMILY: Iron/ascorbate-dependent oxidoreductase family ORGANISM: Papaver somniferum (Opium poppy) SIMILARITY: Belongs to the iron/ascorbate-dependent oxidoreductase family. FUNCTION: Non-heme dioxygenase involved in biosynthesis of morphinan-type benzylisoquinoline and opiate alkaloids natural products. Mediates the conversion of thebaine to neopinone. Catalyzes also, with lower efficiency, the 6-O-demethylation of oripavine to neomorphinone, which is converted spontaneously to morphinone. Supports dealkylation reactions such as O,O-demethylenation in the metabolism of protopine, benzo[c]phenanthridine, and rhoeadine alkaloids; cleaves a methylenedioxy bridge leaving two hydroxyl groups. Catalyzes the O-demethylation of methylenedioxy bridges on protopine alkaloids such as allocryptopine. No activity with (S)-reticuline, salutaridine, papaverine, (S)-corytuberine, (S)-scoulerine, pavine, noscapine or codeine. PATHWAY: Alkaloid biosynthesis; morphine biosynthesis. KEYWORDS: 3D-structure;Alkaloid metabolism;Dioxygenase;Iron;Metal-binding;Methyltransferase;Oxidoreductase;Reference proteome;Transferase MISCELLANEOUS: Neopinone spontaneously rearranges to the more stable codeinone." D5EY13,"PROTEIN NAMES: Endo-1,4-beta-xylanase/feruloyl esterase [Includes: Endo-1,4-beta-xylanase ; Feruloyl esterase (Ferulic acid esterase)] PROTEIN FAMILY: Glycosyl hydrolase 10 (cellulase F) family ORGANISM: Xylanibacter ruminicola (strain ATCC 19189 / DSM 19721 / CIP 105475 / JCM 8958 / 23) (Prevotella ruminicola) SIMILARITY: In the N-terminal section; belongs to the glycosyl hydrolase 10 (cellulase F) family. FUNCTION: Involved in degradation of plant cell wall polysaccharides. Has endo-xylanase activity towards substrates such as oat spelt xylan (OSX), acetylated xylo-oligosaccharides and acetylated xylan, producing primarily xylobiose; cannot hydrolyze xylobiose to xylose. Also has feruloyl esterase activity, releasing ferulic acid from methylferulate, and from the more natural substrates wheat bran, corn fiber, and XOS(FA,Ac), a corn fiber-derived substrate enriched in O-acetyl and ferulic acid esters. Exhibits negligible acetyl esterase activity on sugar acetates. Acts synergistically with Xyl3A to increase the release of xylose from xylan. Does not possess endoglucanase or mannanase activities since it is not able to hydrolyze carboxymethyl cellulose and locust bean gum. PATHWAY: Glycan degradation; xylan degradation. KEYWORDS: Carbohydrate metabolism;Glycosidase;Hydrolase;Multifunctional enzyme;Polysaccharide degradation;Reference proteome;Signal;Xylan degradation " D5EY15,"PROTEIN NAMES: Xylan 1,4-beta-xylosidase (1,4-beta-D-xylan xylohydrolase) (Alpha-L-arabinofuranosidase) (Arabinosidase) (Beta-D-xylosidase) (Exo-1,4-beta-xylosidase) PROTEIN FAMILY: Glycosyl hydrolase 3 family ORGANISM: Xylanibacter ruminicola (strain ATCC 19189 / DSM 19721 / CIP 105475 / JCM 8958 / 23) (Prevotella ruminicola) SIMILARITY: Belongs to the glycosyl hydrolase 3 family. FUNCTION: Involved in degradation of plant cell wall polysaccharides. Has beta-xylosidase activity via its capacity to hydrolyze glycosidic linkages of beta-1,4-xylo-oligosaccharides of various lengths (X2 to X6), releasing xylose monomers. To a much lesser extent, also has alpha-L-arabinofuranosidase activity. Does not possess beta-D-glucosidase activity. Acts synergistically with Xyn10D-Fae1A to increase the release of xylose from xylan. PATHWAY: Glycan degradation; xylan degradation. KEYWORDS: Carbohydrate metabolism;Hydrolase;Polysaccharide degradation;Reference proteome;Signal;Xylan degradation " D5MP61,"PROTEIN NAMES: Beta-1,3-xylanase XYL4 (Beta-1,3-xylanase) PROTEIN FAMILY: Glycosyl hydrolase 26 family ORGANISM: Vibrio sp SIMILARITY: Belongs to the glycosyl hydrolase 26 family. FUNCTION: Catalyzes the hydrolysis of beta-1,3-xylan into oligosaccharides, mainly xylobiose, xylotriose and xylotetraose. Converts beta-1,3-xylotriose into xylose and xylobiose, converts beta-1,3-xylotetraose mainly into xylotriose and xylose, converts beta-1,3-xylopentaose into xylobiose and xylotriose. Does not hydrolyze beta-1,4-xylan, beta-1,4-mannan, beta-1,4-glucan, beta-1,3-xylobiose or p-nitrophenyl-beta-xyloside. KEYWORDS: 3D-structure;Carbohydrate metabolism;Cellulose degradation;Disulfide bond;Glycosidase;Hydrolase;Polysaccharide degradation;Repeat;Signal;Xylan degradation " D6MZJ6,PROTEIN NAMES: Sphingomyelin phosphodiesterase 5 (Mitochondrial neutral sphingomyelinase) (mtnSMase) PROTEIN FAMILY: Neutral sphingomyelinase family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the neutral sphingomyelinase family. FUNCTION: Catalyzes the hydrolysis of membrane sphingomyelin to form phosphorylcholine and ceramide. PATHWAY: Lipid metabolism; sphingolipid metabolism. KEYWORDS: Endoplasmic reticulum;Hydrolase;Lipid metabolism;Magnesium;Membrane;Metal-binding;Mitochondrion;Mitochondrion inner membrane;Reference proteome;Signal-anchor;Sphingolipid metabolism;Transit peptide;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Mitochondrion inner membrane ; Single-pass type II membrane protein ; Intermembrane side Endoplasmic reticulum membrane ; Single-pass membrane protein D7UNT2,"PROTEIN NAMES: L-carnitine dehydrogenase (CDH) (L-CDH) PROTEIN FAMILY: 3-hydroxyacyl-CoA dehydrogenase family, L-carnitine dehydrogenase subfamily ORGANISM: Rhizobium sp SIMILARITY: In the N-terminal section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family. L-carnitine dehydrogenase subfamily. FUNCTION: Catalyzes the NAD(+)-dependent oxidation of L-carnitine to 3-dehydrocarnitine. Despite a high similarity to 3-hydroxyacyl-CoA dehydrogenases, cannot dehydrogenate 3-hydroxybutylate and 3-hydroxybutyl-CoA. Is probably involved in a L-carnitine degradation pathway that allows Rhizobium sp. YS-240 to grow on L-carnitine as the sole source of carbon and nitrogen. PATHWAY: Amine and polyamine metabolism; carnitine metabolism. KEYWORDS: Cytoplasm;Direct protein sequencing;NAD;Oxidoreductase SUBCELLULAR LOCATION: Cytoplasm " D7Y2H2,"PROTEIN NAMES: Cyclic AMP-AMP-AMP synthase (CD-NTase018) (c-di-AMP synthase) PROTEIN FAMILY: CD-NTase family, A01 subfamily ORGANISM: Escherichia coli (strain MS 115-1) SIMILARITY: Belongs to the CD-NTase family. A01 subfamily. FUNCTION: Cyclic nucleotide synthase (second messenger synthase) of a CBASS antivirus system. CBASS (cyclic oligonucleotide-based antiphage signaling system) provides immunity against bacteriophage. The CD-NTase protein synthesizes cyclic nucleotides in response to infection; these serve as specific second messenger signals. The signals activate a diverse range of effectors, leading to bacterial cell death and thus abortive phage infection. A type III-C(AAA) CBASS system.; FUNCTION: Cyclic nucleotide synthase that upon activation catalyzes the synthesis of 3',3',3'-cyclic AMP-AMP-AMP (3',3',3'-c-tri-AMP or cAAA) as the major product, and 3',3'-c-di-AMP as a minor product. Cannot use GTP as a substrate.; FUNCTION: Protects E.coli strain JP313 against bacteriophage lambda cI- infection. When the cdnC-cap7-cap6-nucC operon is transformed into a susceptible strain it confers bacteriophage immunity. Mutations in the sensor (Cap7 also called HORMA) or effector proteins (CdnC, NucC) but not the disassembly protein (Cap6 also called Trip13) no longer confer immunity. The presence of the intact operon leads to culture collapse and cell death, which occurs before the phage has finished its replication cycle, thus protecting non-infected bacteria by aborting the phage infection and preventing its propagation. KEYWORDS: 3D-structure;Antiviral defense;ATP-binding;Magnesium;Metal-binding;Nucleotide metabolism;Nucleotide-binding;Nucleotidyltransferase;Transferase " D8QTR2,PROTEIN NAMES: Mannosylglycerate hydrolase MGH1 PROTEIN FAMILY: Glycosyl hydrolase 63 family ORGANISM: Selaginella moellendorffii (Spikemoss) SIMILARITY: Belongs to the glycosyl hydrolase 63 family. FUNCTION: Catalyzes the hydrolysis of alpha-D-mannosyl-glycerate (MG) to D-glycerate and D-mannose. Can also hydrolyze alpha-D-glucopyranosyl-glycerate (GG)with lower efficiency. KEYWORDS: Glycosidase;Hydrolase;Reference proteome D9TT09,"PROTEIN NAMES: Sucrose 6(F)-phosphate phosphorylase (Sucrose 6'-phosphate phosphorylase) (SPP) PROTEIN FAMILY: Glycosyl hydrolase 13 family, Sucrose phosphorylase subfamily ORGANISM: Thermoanaerobacterium thermosaccharolyticum (strain ATCC 7956 / DSM 571 / NCIMB 9385 / NCA 3814 / NCTC 13789 / WDCM 00135 / 2032) (Clostridium thermosaccharolyticum) SIMILARITY: Belongs to the glycosyl hydrolase 13 family. Sucrose phosphorylase subfamily. FUNCTION: Catalyzes the reversible phosphorolysis of sucrose 6(F)-phosphate into alpha-D-glucose 1-phosphate (Glc1P) and D-fructose 6-phosphate. May be involved in a new pathway for the degradation of sucrose, which could become phosphorylated on its fructose moiety during uptake via a PTS system. To a lesser extent, can also reversibly act on sucrose in vitro. Is also able to catalyze transglycosylation reactions in vitro. KEYWORDS: 3D-structure;Carbohydrate metabolism;Glycosyltransferase;Reference proteome;Transferase " E0D877,PROTEIN NAMES: Apyrase (ATP-diphosphatase) (ADPase) (ATP-diphosphohydrolase) (Adenosine diphosphatase) (allergen Aed al 1) PROTEIN FAMILY: 5'-nucleotidase family ORGANISM: Aedes albopictus (Asian tiger mosquito) (Stegomyia albopicta) SIMILARITY: Belongs to the 5'-nucleotidase family. FUNCTION: Facilitates hematophagy by inhibiting ADP-dependent platelet aggregation in the host. Cleaves adenosine triphosphate (ATP) and adenosine diphosphate (ADP) to adenosine monophosphate (AMP) and inorganic phosphate. May reduce probing time by facilitating the speed of locating blood. KEYWORDS: Allergen;ATP-binding;Direct protein sequencing;Glycoprotein;Hemostasis impairing toxin;Hydrolase;Metal-binding;Nucleotide-binding;Platelet aggregation inhibiting toxin;Secreted;Signal;Toxin SUBCELLULAR LOCATION: Secreted MISCELLANEOUS: Platelet aggregation is inhibited by 6% when 0.4 uM recombinant apyrase is added and by 9.5% when the concentration of recombinant apyrase is 0.8 uM. E0Y3X1,"PROTEIN NAMES: Deoxybrevianamide E synthase notF (Reverse prenyltransferase notF) PROTEIN FAMILY: Tryptophan dimethylallyltransferase family ORGANISM: Aspergillus sp. (strain MF297-2) SIMILARITY: Belongs to the tryptophan dimethylallyltransferase family. FUNCTION: Deoxybrevianamide E synthase; part of the gene cluster that mediates the biosynthesis of notoamide, a fungal indole alkaloid that belongs to a family of natural products containing a characteristic bicyclo[2.2.2]diazaoctane core. The first step of notoamide biosynthesis involves coupling of L-proline and L-tryptophan by the bimodular NRPS notE, to produce cyclo-L-tryptophan-L-proline called brevianamide F. The reverse prenyltransferase notF then acts as a deoxybrevianamide E synthase and converts brevianamide F to deoxybrevianamide E via reverse prenylation at C-2 of the indole ring leading to the bicyclo[2.2.2]diazaoctane core. Deoxybrevianamide E is further hydroxylated at C-6 of the indole ring, likely catalyzed by the cytochrome P450 monooxygenase notG, to yield 6-hydroxy-deoxybrevianamide E (Probable). 6-hydroxy-deoxybrevianamide E is a specific substrate of the prenyltransferase notC for normal prenylation at C-7 to produce 6-hydroxy-7-prenyl-deoxybrevianamide, also called notoamide S. As the proposed pivotal branching point in notoamide biosynthesis, notoamide S can be diverted to notoamide E through an oxidative pyran ring closure putatively catalyzed by either notH cytochrome P450 monooxygenase or the notD FAD-linked oxidoreductase (Probable). This step would be followed by an indole 2,3-epoxidation-initiated pinacol-like rearrangement catalyzed by the notB FAD-dependent monooxygenase leading to the formation of notoamide C and notoamide D. On the other hand notoamide S is converted to notoamide T by notH (or notD), a bifunctional oxidase that also functions as the intramolecular Diels-Alderase responsible for generation of (+)-notoamide T (Probable). To generate antipodal (-)-notoaminide T, notH' (or notD') in Aspergillus versicolor is expected to catalyze a Diels-Alder reaction leading to the opposite stereochemistry (Probable). The remaining oxidoreductase notD (or notH) likely catalyzes the oxidative pyran ring formation to yield (+)-stephacidin A (Probable). The FAD-dependent monooxygenase notI is highly similar to notB and is predicted to catalyze a similar conversion from (+)-stephacidin A to (-)-notoamide B via the 2,3-epoxidation of (+)-stephacidin A followed by a pinacol-type rearrangement (Probable). Finally, it remains unclear which enzyme could be responsible for the final hydroxylation steps leading to notoamide A and sclerotiamide (Probable). PATHWAY: Alkaloid biosynthesis. KEYWORDS: 3D-structure;Alkaloid metabolism;Prenyltransferase;Transferase " E1BTG2,"PROTEIN NAMES: Leiomodin-2 (Cardiac leiomodin) (C-LMOD) PROTEIN FAMILY: Tropomodulin family ORGANISM: Gallus gallus (Chicken) SIMILARITY: Belongs to the tropomodulin family. FUNCTION: Mediates nucleation of actin filaments and thereby promotes actin polymerization (By similarity). Plays a role in the regulation of actin filament length. Required for normal sarcomere organization in the heart, and for normal heart function (By similarity). KEYWORDS: Actin-binding;Coiled coil;Cytoplasm;Cytoskeleton;Reference proteome SUBCELLULAR LOCATION: Cytoplasm, myofibril, sarcomere Cytoplasm, myofibril Cytoplasm, myofibril, sarcomere, M line Cytoplasm, cytoskeleton Note=Colocalizes with actin filament pointed ends in sarcomeres. Detected close to the M line. " E1XUJ2,"PROTEIN NAMES: Linalool dehydratase/isomerase (Geraniol isomerase) (Linalool dehydratase-isomerase) (Myrcene hydratase) ORGANISM: Castellaniella defragrans (strain DSM 12143 / CCUG 39792 / 65Phen) (Alcaligenes defragrans) FUNCTION: Anaerobically catalyzes the stereospecific hydration of beta-myrcene to (3S)-linalool and the isomerization of (3S)-linalool to geraniol. Is thus involved in the initial steps of the anaerobic degradation of the monoterpene beta-myrcene. Also catalyzes the reverse reactions, i.e. the isomerization of geraniol to linalool and the dehydration of linalool to myrcene. In this direction, the formation of myrcene from geraniol may be seen as a detoxification process for the monoterpene alcohol. Shows a relatively broad substrate specificity and can use various geraniol and linalool derivatives. Substrates required a specific alpha-methylallyl alcohol signature motif. Neither the monoterpenes alpha- and beta-ocimene nor the monoterpenoids citronellol and nerol can be used as substrates. PATHWAY: Terpene metabolism; monoterpene degradation. KEYWORDS: 3D-structure;Direct protein sequencing;Disulfide bond;Isomerase;Lyase;Periplasm;Reference proteome;Signal SUBCELLULAR LOCATION: Periplasm MISCELLANEOUS: Requires DTT as a reducing agent for full activity in vitro." E3PY95,"PROTEIN NAMES: D-ornithine 4,5-aminomutase subunit beta (D-ornithine aminomutase E component) (OAM-E) ORGANISM: Acetoanaerobium sticklandii (strain ATCC 12662 / DSM 519 / JCM 1433 / CCUG 9281 / NCIMB 10654 / HF) (Clostridium sticklandii) FUNCTION: Component of a complex that catalyzes the reversible migration of the omega amino group of D-ornithine to C-4 to form (2R,4S)-2,4-diaminopentanoic acid. OraE may be the catalytic subunit. Active only on D-ornithine and 2,4-diaminopentanoic acid but not active on L-ornithine, L-beta-lysine, L-alpha-lysine or D-alpha-lysine. KEYWORDS: 3D-structure;Cobalamin;Cobalt;Direct protein sequencing;Isomerase;Metal-binding;Pyridoxal phosphate;Reference proteome " E4MYY0,"PROTEIN NAMES: (2Z,6E)-hedycaryol synthase (HcS) PROTEIN FAMILY: Terpene synthase family ORGANISM: Kitasatospora setae (strain ATCC 33774 / DSM 43861 / JCM 3304 / KCC A-0304 / NBRC 14216 / KM-6054) (Streptomyces setae) SIMILARITY: Belongs to the terpene synthase family. FUNCTION: Catalyzes the conversion of (2E,6E)-farnesyl diphosphate (FPP) into (2Z,6E)-hedycaryol via a 1,11-cyclization. PATHWAY: Secondary metabolite biosynthesis; terpenoid biosynthesis. KEYWORDS: 3D-structure;Lyase;Magnesium;Metal-binding;Reference proteome MISCELLANEOUS: The 1,11-cyclization requires isomerization of (2E,6E)-farnesyl diphosphate (FPP) to nerolidyl diphosphate (NPP), the abstraction of the pyrophosphate from intermediate NPP leading to a (Z,E)-hedycaryl cation and finally an nucleophilic attack of a water molecule." E4QP00,"PROTEIN NAMES: 5-(hydroxymethyl)furfural oxidase (5-hydroxymethylfurfural oxidase) (HMFO) (Thiol oxidase) PROTEIN FAMILY: GMC oxidoreductase family ORGANISM: Methylovorus sp. (strain MP688) SIMILARITY: Belongs to the GMC oxidoreductase family. FUNCTION: Involved in the degradation and detoxification of 5-(hydroxymethyl)furfural (HMF) by mediating its oxidation to furan-2,5-dicarboxylate (FDCA), a biobased platform chemical for the production of polymers. Active with a wide range of aromatic and aliphatic primary alcohols and aldehydes: acts on alcohol groups and requires the spontaneous hydration of aldehyde groups for their oxidation. To a lesser extent, is also able to catalyze the oxidation of thiols that are structurally similar to its alcohol substrates, yielding the corresponding thiocarbonyls. KEYWORDS: 3D-structure;FAD;Flavoprotein;Oxidoreductase MISCELLANEOUS: At pH 7.0, phenylmethanethiol is oxidized to the corresponding aromatic thioaldehyde, benzothialdehyde, and no formation of 1,2-dibenzyldisulfane is observed, demonstrating that HMFO does not catalyze the formation of disulfide bonds. At pH 8.0, two products are formed, benzothialdehyde and benzaldehyde; this suggests that the thioaldehyde is slowly hydrated, yielding the aldehyde as the final product." E5RQA1,PROTEIN NAMES: Transcription factor GHD7 (Protein GRAIN NUMBER PLANT HEIGHT AND HEADING DATE 7) ORGANISM: Oryza sativa subsp. japonica (Rice) FUNCTION: Probable transcription factor involved in the regulation of flowering time under long day (LD) conditions. Plays a major role as repressor of flowering. Controls flowering time by negatively regulating the expression of EHD1 and HD3A. KEYWORDS: DNA-binding;Flowering;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus E6LHV7,"PROTEIN NAMES: CRISPR system single-strand-specific deoxyribonuclease Cas10/Csm1 (subtype III-A) (ssDNase Cas10) (Cyclic oligoadenylate synthase) (EiCas10) PROTEIN FAMILY: CRISPR-associated Cas10/Csm1 family ORGANISM: Enterococcus italicus (strain DSM 15952 / CCUG 50447 / LMG 22039 / TP 1.5) SIMILARITY: Belongs to the CRISPR-associated Cas10/Csm1 family. FUNCTION: CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). The type III-A Csm effector complex binds crRNA and acts as a crRNA-guided RNase, DNase and cyclic oligoadenylate synthase; binding of target RNA cognate to the crRNA is required for all activities. In a heterologous host the appropriately targeted Csm effector complex prevents growth of dsDNA phage phiNM1-gamma6.; FUNCTION: ssDNase activity is stimulated in the ternary Csm effector complex; binding of cognate target RNA activates the ssDNase, as the target RNA is degraded ssDNA activity decreases.; FUNCTION: This subunit is a single-strand-specific deoxyribonuclease (ssDNase) which digests both linear and circular ssDNA; it has both exo- and endonuclease activity.; FUNCTION: When associated with the ternary Csm effector complex (the crRNA, Cas proteins and a cognate target ssRNA) synthesizes cyclic oligoadenylates (cOA) from ATP, producing (mostly) cyclic hexaadenylate (cA6). cA6 synthesis occurs in the Csm effector complex and requires cognate target RNA and ATP; other NTPs are not incorporated. cOAs are second messengers that induce an antiviral state important for defense against invading nucleic acids. KEYWORDS: Antiviral defense;ATP-binding;Endonuclease;Exonuclease;Hydrolase;Nuclease;Nucleotide-binding;Reference proteome;RNA-binding;Transferase MISCELLANEOUS: Encoded in a type III-A CRISPR locus." E6Y2X0,"PROTEIN NAMES: Bifunctional pinoresinol-lariciresinol reductase 2 (PLR-Lu2) ((+)-lariciresinol reductase) ((+)-pinoresinol reductase) PROTEIN FAMILY: NmrA-type oxidoreductase family, Isoflavone reductase subfamily ORGANISM: Linum usitatissimum (Flax) (Linum humile) SIMILARITY: Belongs to the NmrA-type oxidoreductase family. Isoflavone reductase subfamily. FUNCTION: Reductase involved in lignan biosynthesis. Catalyzes the enantioselective conversion of (+)-pinoresinol into (+)-lariciresinol and of (+)-lariciresinol into (-)-secoisolariciresinol. Abstracts the 4R-hydride from the NADPH cofactor during catalysis. KEYWORDS: NADP;Oxidoreductase " E6Z0R3,"PROTEIN NAMES: Protein adenylyltransferase VbhT (AMPylator VbhT) (Toxin VbhT) ORGANISM: Bartonella schoenbuchensis (strain DSM 13525 / NCTC 13165 / R1) FUNCTION: Toxic component of type II toxin-antitoxin (TA) system VbhT-VbhA. Adenylyltransferase involved in virulence by mediating the addition of adenosine 5'-monophosphate (AMP) to specific residue of host GTPases. The resulting AMPylation affects GTPases, impairing actin assembly in infected cells. KEYWORDS: 3D-structure;ATP-binding;Nucleotide-binding;Nucleotidyltransferase;Toxin-antitoxin system;Transferase;Virulence MISCELLANEOUS: Defined as class I fido-domain containing proteins, in which the inhibitory helix is provided by an interacting antitoxin (VbhA)." E7F1H9,"PROTEIN NAMES: YTH domain-containing family protein 2 PROTEIN FAMILY: YTHDF family, YTHDF2 subfamily ORGANISM: Danio rerio (Zebrafish) (Brachydanio rerio) SIMILARITY: Belongs to the YTHDF family. YTHDF2 subfamily. FUNCTION: Specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs, and regulates their stability. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in mRNA stability and processing (By similarity). Acts as a regulator of mRNA stability by promoting degradation of m6A-containing mRNAs (By similarity). The YTHDF paralogs (ythdf1, ythdf2 and ythdf3) share m6A-containing mRNAs targets and act redundantly to mediate mRNA degradation and cellular differentiation (By similarity). Plays a key role in maternal-to-zygotic transition during early embryonic development, the process during which maternally inherited mRNAs are degraded: acts by binding m6A-containing maternal mRNAs and promoting their degradation. More than one-third of maternal mRNAs can be modified by m6A. Binding to m6A-containing mRNAs results in mRNA degradation (By similarity). Also involved in hematopoietic stem cells specification by binding to m6A-containing mRNAs, such as notch1a, and promote their degradation. The decreased Notch signaling following notch1a degradation promotes endothelial to hematopoietic transition. Promotes formation of phase-separated membraneless compartments, such as P-bodies or stress granules, by undergoing liquid-liquid phase separation upon binding to mRNAs containing multiple m6A-modified residues: polymethylated mRNAs act as a multivalent scaffold for the binding of YTHDF proteins, juxtaposing their disordered regions and thereby leading to phase separation (By similarity). The resulting mRNA-YTHDF complexes then partition into different endogenous phase-separated membraneless compartments, such as P-bodies, stress granules or neuronal RNA granules (By similarity). KEYWORDS: Alternative splicing;Cytoplasm;Nucleus;Reference proteome;RNA-binding SUBCELLULAR LOCATION: Cytoplasm, cytosol Cytoplasm, P-body Cytoplasm, Stress granule Nucleus Note=Localizes to the cytosol and relocates to the nucleus following heat shock stress. Can partition into different structures: into P-bodies in unstressed cells, and into stress granules during stress. " E7F3I6,"PROTEIN NAMES: Polyribonucleotide 5'-hydroxyl-kinase Clp1 (Polyadenylation factor Clp1) (Polynucleotide kinase Clp1) (Pre-mRNA cleavage complex II protein Clp1) PROTEIN FAMILY: Clp1 family, Clp1 subfamily ORGANISM: Danio rerio (Zebrafish) (Brachydanio rerio) SIMILARITY: Belongs to the Clp1 family. Clp1 subfamily. FUNCTION: Polynucleotide kinase that can phosphorylate the 5'-hydroxyl groups of double-stranded RNA (dsRNA), single-stranded RNA (ssRNA), double stranded DNA (dsDNA) and double-stranded DNA:RNA hybrids. dsRNA is phosphorylated more efficiently than dsDNA, and the RNA component of a DNA:RNA hybrid is phosphorylated more efficiently than the DNA component. Plays a role in both tRNA splicing and mRNA 3'-end formation. Component of the tRNA splicing endonuclease complex: phosphorylates the 5'-terminus of the tRNA 3'-exon during tRNA splicing; this phosphorylation event is a prerequisite for the subsequent ligation of the two exon halves and the production of a mature tRNA. Its role in tRNA splicing and maturation is required for cerebellar development. Component of the pre-mRNA cleavage complex II (CF-II), which seems to be required for mRNA 3'-end formation. Also phosphorylates the 5'-terminus of exogenously introduced short interfering RNAs (siRNAs), which is a necessary prerequisite for their incorporation into the RNA-induced silencing complex (RISC). However, endogenous siRNAs and microRNAs (miRNAs) that are produced by the cleavage of dsRNA precursors by dicer1 already contain a 5'-phosphate group, so this protein may be dispensible for normal RNA-mediated gene silencing. KEYWORDS: ATP-binding;Kinase;mRNA processing;Neurodegeneration;Nucleotide-binding;Nucleus;Reference proteome;Transferase;tRNA processing SUBCELLULAR LOCATION: Nucleus " E7F4N7,"PROTEIN NAMES: Stimulator of interferon genes protein (STING) (Transmembrane protein 173) PROTEIN FAMILY: STING family ORGANISM: Danio rerio (Zebrafish) (Brachydanio rerio) SIMILARITY: Belongs to the STING family. FUNCTION: Facilitator of innate immune signaling that acts as a sensor of cytosolic DNA from bacteria and viruses and promotes the production of type I interferon (IFN-alpha and IFN-beta). Innate immune response is triggered in response to non-CpG double-stranded DNA from viruses and bacteria delivered to the cytoplasm. Acts by binding cyclic dinucleotides: recognizes and binds cyclic di-GMP (c-di-GMP), a second messenger produced by bacteria, and cyclic GMP-AMP (cGAMP), a messenger produced by CGAS in response to DNA virus in the cytosol (By similarity). Upon binding of c-di-GMP or cGAMP, STING1 oligomerizes and is able to activate both NF-kappa-B and irf3 transcription pathways to induce expression of type I interferon and exert a potent anti-viral state. Exhibits 2',3' phosphodiester linkage-specific ligand recognition: can bind both 2'-3' linked cGAMP and 3'-3' linked cGAMP but is preferentially activated by 2'-3' linked cGAMP (By similarity). In addition to promote the production of type I interferons, plays a direct role in autophagy. Following cGAMP-binding, STING1 buds from the endoplasmic reticulum into COPII vesicles, which then form the endoplasmic reticulum-Golgi intermediate compartment (ERGIC) (By similarity). The ERGIC serves as the membrane source for LC3 lipidation, leading to formation of autophagosomes that target cytosolic DNA or DNA viruses for degradation by the lysosome (By similarity). Promotes autophagy by acting as a proton channel that directs proton efflux from the Golgi to facilitate LC3 lipidation (By similarity). The autophagy- and interferon-inducing activities can be uncoupled and autophagy induction is independent of TBK1 phosphorylation (By similarity). KEYWORDS: 3D-structure;Cytoplasm;Cytoplasmic vesicle;Endoplasmic reticulum;Golgi apparatus;Immunity;Innate immunity;Ion channel;Ion transport;Isopeptide bond;Membrane;Nucleotide-binding;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein Cytoplasm, perinuclear region Endoplasmic reticulum-Golgi intermediate compartment membrane ; Multi-pass membrane protein Golgi apparatus membrane ; Multi-pass membrane protein Cytoplasmic vesicle, autophagosome membrane ; Multi-pass membrane protein Note=In response to double-stranded DNA stimulation, translocates from the endoplasmic reticulum through the endoplasmic reticulum-Golgi intermediate compartment and Golgi to post-Golgi vesicles, where the kinase tbk1 is recruited. Upon cGAMP-binding, translocates to the endoplasmic reticulum-Golgi intermediate compartment (ERGIC) in a process that is dependent on COPII vesicles; STING1-containing ERGIC serves as a membrane source for LC3 lipidation, which is a key step in autophagosome biogenesis. " E7FHC4,PROTEIN NAMES: Sulfhydrogenase 2 subunit alpha (Hydrogen dehydrogenase (NAD(P)(+))) (Hydrogenase-II subunit alpha) (H-II alpha) (NADP-reducing hydrogenase subunit ShyA) (Sulfhydrogenase II subunit alpha) PROTEIN FAMILY: [NiFe]/[NiFeSe] hydrogenase large subunit family ORGANISM: Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) SIMILARITY: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family. FUNCTION: Part of a bifunctional enzyme complex that functions as a hydrogen-evolving hydrogenase with sulfur-reducing activity. May play a role in hydrogen cycling during fermentative growth. Activity exhibited with NAD in addition to NADPH. The alpha and delta subunits form the hydrogenase component that catalyzes the reduction of protons to evolve hydrogen. KEYWORDS: Cytoplasm;Direct protein sequencing;Iron;Metal-binding;NAD;NADP;Nickel;Oxidoreductase;Reference proteome SUBCELLULAR LOCATION: Cytoplasm E7FHU4,PROTEIN NAMES: Sulfhydrogenase 1 subunit delta (Hydrogenase I small subunit) (NADP-reducing hydrogenase subunit HydD) (Sulfhydrogenase I subunit delta) PROTEIN FAMILY: [NiFe]/[NiFeSe] hydrogenase small subunit family ORGANISM: Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) SIMILARITY: Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family. FUNCTION: Part of a bifunctional enzyme complex that functions as an NADPH-dependent hydrogen-evolving hydrogenase with sulfur reducing activity. May play a role in hydrogen cycling during fermentative growth. Activity not exhibited with NAD. The alpha and delta subunits form the hydrogenase component that catalyzes the reduction of protons to evolve hydrogen. KEYWORDS: 3Fe-4S;4Fe-4S;Cytoplasm;Direct protein sequencing;Iron;Iron-sulfur;Metal-binding;NADP;Nickel;Oxidoreductase;Reference proteome SUBCELLULAR LOCATION: Cytoplasm E8MGH8,"PROTEIN NAMES: Non-reducing end beta-L-arabinofuranosidase (Beta-L-arabinofuranosidase) (Beta-AFase) PROTEIN FAMILY: Glycosyl hydrolase 127 family ORGANISM: Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b) SIMILARITY: Belongs to the glycosyl hydrolase 127 family. FUNCTION: Beta-L-arabinofuranosidase that removes the beta-L-arabinofuranose residue from the non-reducing end of various substrates, including beta-L-arabinofuranosyl-hydroxyproline (Ara-Hyp), Ara-beta-1,2-Ara-beta-Hyp (Ara(2)-Hyp), Ara-beta-1,2-Ara-beta-1,2-Ara-beta-Hyp (Ara(3)-Hyp), and beta-L-arabinofuranosyl-(1->2)-1-O-methyl-beta-L-arabinofuranose. In the presence of 1-alkanols, shows transglycosylation activity, retaining the anomeric configuration of the arabinofuranose residue. KEYWORDS: 3D-structure;Carbohydrate metabolism;Glycosidase;Hydrolase;Metal-binding;Polysaccharide degradation;Zinc " E9P8D2,"PROTEIN NAMES: N-acetyltransferase MPR1 ((S)-1-pyrroline-5-carboxylate acetyltransferase) (L-azetidine-2-carboxylate acetyltransferase) (AZC acetyltransferase) (Sigma1278b gene for proline-analog resistance 1) PROTEIN FAMILY: Acetyltransferase family ORGANISM: Saccharomyces cerevisiae (Baker's yeast) SIMILARITY: Belongs to the acetyltransferase family. FUNCTION: N-acetyltransferase involved in oxidative stress resistance. Acetylates the toxic proline metabolism intermediate (S)-1-pyrroline-5-carboxylate (P5C), or more likely its spontaneously forming tautomer glutamate-5-semialdehyde (GSA) into N-acetyl-GSA for arginine synthesis in the mitochondria. P5C has been shown to increase the levels of reactive oxygen species (ROS) in the cell by inhibiting the function of the respiratory chain in the mitochondria. The enzyme is able to reduce intracellular ROS levels under P5C-induced oxidative stress and protects cells from damage by oxidative stress. Also acetylates and thereby detoxifies the proline analog azetidine-2-carboxylate (AZC), however it is unlikely that AZC is a natural substrate as it occurs only in plants belonging to the Lilaceae family. Does not acetylate proline. KEYWORDS: 3D-structure;Acyltransferase;Cytoplasm;Mitochondrion;Transferase SUBCELLULAR LOCATION: Cytoplasm Mitochondrion " E9Q649,"PROTEIN NAMES: Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 4 (Core 2-branching enzyme 3) (Core2-GlcNAc-transferase 3) (C2GnT3) PROTEIN FAMILY: Glycosyltransferase 14 family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the glycosyltransferase 14 family. FUNCTION: Glycosyltransferase that mediates core 2 O-glycan branching, an important step in mucin-type biosynthesis. Does not have core 4 O-glycan or I-branching enzyme activity. PATHWAY: Protein modification; protein glycosylation. KEYWORDS: Disulfide bond;Glycoprotein;Glycosyltransferase;Golgi apparatus;Membrane;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus membrane ; Single-pass type II membrane protein " E9Q816,"PROTEIN NAMES: Cytochrome P450 2W1 PROTEIN FAMILY: Cytochrome P450 family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the cytochrome P450 family. FUNCTION: A cytochrome P450 monooxygenase that may play a role in retinoid and phospholipid metabolism. Catalyzes the hydroxylation of saturated carbon hydrogen bonds. Hydroxylates all trans-retinoic acid (atRA) to 4-hydroxyretinoate and may regulate atRA clearance. Other retinoids such as all-trans retinol and all-trans retinal are potential endogenous substrates. Catalyzes both epoxidation of double bonds and hydroxylation of carbon hydrogen bonds of the fatty acyl chain of 1-acylphospholipids/2-lysophospholipids. Can metabolize various lysophospholipids classes including lysophosphatidylcholines (LPCs), lysophosphatidylinositols (LPIs), lysophosphatidylserines (LPSs), lysophosphatidylglycerols (LPGs), lysophosphatidylethanolamines (LPEs) and lysophosphatidic acids (LPAs). Has low or no activity toward 2-acylphospholipids/1-lysophospholipids, diacylphospholipids and free fatty acids. May play a role in tumorigenesis by activating procarcinogens such as aflatoxin B1, polycyclic aromatic hydrocarbon dihydrodiols and aromatic amines. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase). KEYWORDS: Cell membrane;Endoplasmic reticulum;Glycoprotein;Heme;Iron;Lipid metabolism;Membrane;Metal-binding;Microsome;Monooxygenase;Oxidoreductase;Reference proteome;Signal SUBCELLULAR LOCATION: Endoplasmic reticulum lumen Cell membrane Microsome membrane " E9QA28,"PROTEIN NAMES: Carcinoembryonic antigen-related cell adhesion molecule 16 PROTEIN FAMILY: Immunoglobulin superfamily, CEA family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the immunoglobulin superfamily. CEA family. FUNCTION: Required for proper hearing, plays a role in maintaining the integrity of the tectorial membrane. KEYWORDS: Disulfide bond;Glycoprotein;Immunoglobulin domain;Reference proteome;Repeat;Secreted;Signal SUBCELLULAR LOCATION: Secreted Note=Localizes at the tip of cochlear outer hair cells and to the tectorial membrane. " E9QJ73,PROTEIN NAMES: C-X-C chemokine receptor type 3-2 PROTEIN FAMILY: G-protein coupled receptor 1 family ORGANISM: Danio rerio (Zebrafish) (Brachydanio rerio) SIMILARITY: Belongs to the G-protein coupled receptor 1 family. FUNCTION: Receptor for the C-X-C chemokines cxcl11.1 and cxcl11.6. Promotes macrophage chemotaxis to sites of bacterial infection. KEYWORDS: Cell membrane;Chemotaxis;Disulfide bond;G-protein coupled receptor;Glycoprotein;Immunity;Innate immunity;Membrane;Receptor;Reference proteome;Transducer;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein E9RAH5,"PROTEIN NAMES: Thioredoxin reductase gliT (Gliotoxin biosynthesis protein T) PROTEIN FAMILY: Class-II pyridine nucleotide-disulfide oxidoreductase family ORGANISM: Aspergillus fumigatus (strain ATCC MYA-4609 / CBS 101355 / FGSC A1100 / Af293) (Neosartorya fumigata) SIMILARITY: Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family. FUNCTION: Thioredoxin reductase; part of the gene cluster that mediates the biosynthesis of gliotoxin, a member of the epipolythiodioxopiperazine (ETP) class of toxins characterized by a disulfide bridged cyclic dipeptide. The first step in gliotoxin biosynthesis is the condensation of serine and phenylalanine to form the cyclo-L-phenylalanyl-L-serine diketopiperazine (DKP) by the NRPS gliP. GliP is also able to produce the DKP cyclo-L-tryptophanyl-L-serine, suggesting that the substrate specificity of the first adenylation (A) domain in gliP is sufficiently relaxed to accommodate both L-Phe and L-Trp. The cytochrome P450 monooxygenase gliC has been shown to catalyze the subsequent hydroxylation of the alpha-carbon of L-Phe in cyclo-L-phenylalanyl-L-serine whereas the second cytochrome P450 enzyme, gliF, is presumably involved in the modification of the DKP side chain. The glutathione S-transferase (GST) gliG then forms a bis-glutathionylated biosynthetic intermediate which is responsible for the sulfurization of gliotoxin. This bis-glutathionylated intermediate is subsequently processed by the gamma-glutamyl cyclotransferase gliK to remove both gamma-glutamyl moieties. Subsequent processing via gliI yields a biosynthetic intermediate, which is N-methylated via the N-methyltransferase gliN, before the gliotoxin oxidoreductase gliT-mediated disulfide bridge closure. GliN-mediated amide methylation confers stability to ETP, damping the spontaneous formation of tri- and tetrasulfides. Intracellular dithiol gliotoxin oxidized by gliT is subsequently effluxed by gliA. GliT is required for self-protection against gliotoxin. GliT plays a role in preventing dysregulation of the methyl/methionine cycle to control intracellular S-adenosylmethionine (SAM) depletion and S-adenosylhomocysteine (SAH) homeostasis during gliotoxin biosynthesis and exposure. PATHWAY: Mycotoxin biosynthesis. KEYWORDS: 3D-structure;Disulfide bond;FAD;Flavoprotein;NADP;Oxidoreductase;Redox-active center;Reference proteome;Virulence " F0JAI6,"PROTEIN NAMES: Kinetochore and Eb1-associated basic protein ORGANISM: Drosophila melanogaster (Fruit fly) KEYWORDS: Alternative splicing;Centromere;Chromosome;Coiled coil;Cytoplasm;Cytoskeleton;Kinetochore;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Cytoplasm, perinuclear region Chromosome, centromere, kinetochore Cytoplasm, cytoskeleton, spindle Note=During metaphase expressed in the kinetochores. During anaphase expression in the kinetochores progressively increases, and at late anaphase it is also expressed in the microtubules, specifically in the central spindle and centrosomal region. During telophase expression increases in the microtubules, and it is associated with residual spindle microtubules between chromosomes that have separated. At interphase it is expressed in the cytoplasm particularly around the nucleus. " F1M391,"PROTEIN NAMES: Stimulator of interferon genes protein (rSTING) (Transmembrane protein 173) PROTEIN FAMILY: STING family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the STING family. FUNCTION: Facilitator of innate immune signaling that acts as a sensor of cytosolic DNA from bacteria and viruses and promotes the production of type I interferon (IFN-alpha and IFN-beta). Innate immune response is triggered in response to non-CpG double-stranded DNA from viruses and bacteria delivered to the cytoplasm (By similarity). Acts by binding cyclic dinucleotides: recognizes and binds cyclic di-GMP (c-di-GMP), a second messenger produced by bacteria, cyclic UMP-AMP (2',3'-cUAMP), and cyclic GMP-AMP (cGAMP), a messenger produced by CGAS in response to DNA virus in the cytosol (By similarity). Upon binding to c-di-GMP, cUAMP or cGAMP, STING1 oligomerizes, translocates from the endoplasmic reticulum and is phosphorylated by TBK1 on the pLxIS motif, leading to recruitment and subsequent activation of the transcription factor IRF3 to induce expression of type I interferon and exert a potent anti-viral state. Exhibits 2',3' phosphodiester linkage-specific ligand recognition: can bind both 2'-3' linked cGAMP (2'-3'-cGAMP) and 3'-3' linked cGAMP but is preferentially activated by 2'-3' linked cGAMP. The preference for 2'-3'-cGAMP, compared to other linkage isomers is probably due to the ligand itself, whichs adopts an organized free-ligand conformation that resembles the STING1-bound conformation and pays low energy costs in changing into the active conformation (By similarity). In addition to promote the production of type I interferons, plays a direct role in autophagy (By similarity). Following cGAMP-binding, STING1 buds from the endoplasmic reticulum into COPII vesicles, which then form the endoplasmic reticulum-Golgi intermediate compartment (ERGIC) (By similarity). The ERGIC serves as the membrane source for WIPI2 recruitment and LC3 lipidation, leading to formation of autophagosomes that target cytosolic DNA or DNA viruses for degradation by the lysosome (By similarity). Promotes autophagy by acting as a proton channel that directs proton efflux from the Golgi to facilitate MAP1LC3B/LC3B lipidation (By similarity). The autophagy- and interferon-inducing activities can be uncoupled and autophagy induction is independent of TBK1 phosphorylation (By similarity). Autophagy is also triggered upon infection by bacteria: following c-di-GMP-binding, which is produced by live Gram-positive bacteria, promotes reticulophagy (By similarity). May be involved in translocon function, the translocon possibly being able to influence the induction of type I interferons (By similarity). May be involved in transduction of apoptotic signals via its association with the major histocompatibility complex class II (MHC-II) (By similarity). KEYWORDS: 3D-structure;Autophagy;Cell membrane;Cytoplasm;Cytoplasmic vesicle;Endoplasmic reticulum;Golgi apparatus;Immunity;Innate immunity;Ion channel;Ion transport;Isopeptide bond;Lipoprotein;Membrane;Mitochondrion;Mitochondrion outer membrane;Nucleotide-binding;Palmitate;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix;Transport;Ubl conjugation SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein Cytoplasm, perinuclear region Endoplasmic reticulum-Golgi intermediate compartment membrane ; Multi-pass membrane protein Golgi apparatus membrane ; Multi-pass membrane protein Cytoplasmic vesicle, autophagosome membrane ; Multi-pass membrane protein Mitochondrion outer membrane ; Multi-pass membrane protein Cell membrane ; Multi-pass membrane protein Note=In response to double-stranded DNA stimulation, translocates from the endoplasmic reticulum through the endoplasmic reticulum-Golgi intermediate compartment and Golgi to post-Golgi vesicles, where the kinase TBK1 is recruited. Upon cGAMP-binding, translocates to the endoplasmic reticulum-Golgi intermediate compartment (ERGIC) in a process that is dependent on COPII vesicles; STING1-containing ERGIC serves as a membrane source for LC3 lipidation, which is a key step in autophagosome biogenesis. Localizes in the lysosome membrane in a TMEM203-dependent manner. " F1M3L7,"PROTEIN NAMES: Epidermal growth factor receptor kinase substrate 8 PROTEIN FAMILY: EPS8 family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the EPS8 family. FUNCTION: Signaling adapter that controls various cellular protrusions by regulating actin cytoskeleton dynamics and architecture. Depending on its association with other signal transducers, can regulate different processes. Together with SOS1 and ABI1, forms a trimeric complex that participates in transduction of signals from Ras to Rac by activating the Rac-specific guanine nucleotide exchange factor (GEF) activity. Acts as a direct regulator of actin dynamics by binding actin filaments and has both barbed-end actin filament capping and actin bundling activities depending on the context. Displays barbed-end actin capping activity when associated with ABI1, thereby regulating actin-based motility process: capping activity is auto-inhibited and inhibition is relieved upon ABI1 interaction. Also shows actin bundling activity when associated with BAIAP2, enhancing BAIAP2-dependent membrane extensions and promoting filopodial protrusions. Involved in the regulation of processes such as axonal filopodia growth, stereocilia length, dendritic cell migration and cancer cell migration and invasion. Acts as a regulator of axonal filopodia formation in neurons: in the absence of neurotrophic factors, negatively regulates axonal filopodia formation via actin-capping activity. In contrast, it is phosphorylated in the presence of BDNF leading to inhibition of its actin-capping activity and stimulation of filopodia formation. Component of a complex with WHRN and MYO15A that localizes at stereocilia tips and is required for elongation of the stereocilia actin core. Indirectly involved in cell cycle progression; its degradation following ubiquitination being required during G2 phase to promote cell shape changes (By similarity). KEYWORDS: Actin-binding;Cell membrane;Cell projection;Cytoplasm;Membrane;Phosphoprotein;Reference proteome;SH3 domain;Synapse;Synaptosome;Ubl conjugation SUBCELLULAR LOCATION: Synapse, synaptosome. Cytoplasm, cell cortex Cell projection, ruffle membrane Cell projection, stereocilium Cell projection, growth cone. Note=Localizes at the tips of the stereocilia of the inner and outer hair cells (By similarity). Localizes to the midzone of dividing cells. " F1N5C8,"PROTEIN NAMES: Glycerophosphocholine choline phosphodiesterase ENPP6 (GPC-Cpde) (Choline-specific glycerophosphodiester phosphodiesterase) (Ectonucleotide pyrophosphatase/phosphodiesterase family member 6) (E-NPP 6) (NPP-6) PROTEIN FAMILY: Nucleotide pyrophosphatase/phosphodiesterase family ORGANISM: Bos taurus (Bovine) SIMILARITY: Belongs to the nucleotide pyrophosphatase/phosphodiesterase family. FUNCTION: Choline-specific glycerophosphodiesterase that hydrolyzes glycerophosphocholine (GPC) and lysophosphatidylcholine (LPC) and contributes to supplying choline to the cells. Has a preference for LPC with short (12:0 and 14:0) or polyunsaturated (18:2 and 20:4) fatty acids. In vitro, hydrolyzes only choline-containing lysophospholipids, such as sphingosylphosphorylcholine (SPC), platelet-activating factor (PAF) and lysoPAF, but not other lysophospholipids (By similarity). KEYWORDS: Cell membrane;Direct protein sequencing;Disulfide bond;Glycoprotein;GPI-anchor;Hydrolase;Lipid degradation;Lipid metabolism;Lipoprotein;Membrane;Metal-binding;Phosphoprotein;Reference proteome;Signal;Zinc SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor, GPI-anchor. " F1PLN3,"PROTEIN NAMES: Alpha-ketoglutarate-dependent dioxygenase FTO (Fat mass and obesity-associated protein) (U6 small nuclear RNA (2'-O-methyladenosine-N(6)-)-demethylase FTO) (U6 small nuclear RNA N(6)-methyladenosine-demethylase FTO) (mRNA (2'-O-methyladenosine-N(6)-)-demethylase FTO) (m6A(m)-demethylase FTO) (mRNA N(6)-methyladenosine demethylase FTO) (tRNA N1-methyl adenine demethylase FTO) PROTEIN FAMILY: Fto family ORGANISM: Canis lupus familiaris (Dog) (Canis familiaris) SIMILARITY: Belongs to the fto family. FUNCTION: RNA demethylase that mediates oxidative demethylation of different RNA species, such as mRNAs, tRNAs and snRNAs, and acts as a regulator of fat mass, adipogenesis and energy homeostasis. Specifically demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes. M6A demethylation by FTO affects mRNA expression and stability. Also able to demethylate m6A in U6 small nuclear RNA (snRNA). Mediates demethylation of N(6),2'-O-dimethyladenosine cap (m6A(m)), by demethylating the N(6)-methyladenosine at the second transcribed position of mRNAs and U6 snRNA. Demethylation of m6A(m) in the 5'-cap by FTO affects mRNA stability by promoting susceptibility to decapping. Also acts as a tRNA demethylase by removing N(1)-methyladenine from various tRNAs. Has no activity towards 1-methylguanine. Has no detectable activity towards double-stranded DNA. Also able to repair alkylated DNA and RNA by oxidative demethylation: demethylates single-stranded RNA containing 3-methyluracil, single-stranded DNA containing 3-methylthymine and has low demethylase activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine. Ability to repair alkylated DNA and RNA is however unsure in vivo. Involved in the regulation of fat mass, adipogenesis and body weight, thereby contributing to the regulation of body size and body fat accumulation. Involved in the regulation of thermogenesis and the control of adipocyte differentiation into brown or white fat cells. Regulates activity of the dopaminergic midbrain circuitry via its ability to demethylate m6A in mRNAs. Plays an oncogenic role in a number of acute myeloid leukemias by enhancing leukemic oncogene-mediated cell transformation: acts by mediating m6A demethylation of target transcripts such as MYC, CEBPA, ASB2 and RARA, leading to promote their expression. KEYWORDS: Acetylation;Cytoplasm;Dioxygenase;Iron;Metal-binding;Nucleus;Oxidoreductase;Phosphoprotein;Reference proteome;Transferase SUBCELLULAR LOCATION: Nucleus Nucleus speckle Cytoplasm Note=Localizes mainly in the nucleus, where it is able to demethylate N(6)-methyladenosine (m6A) and N(6),2'-O-dimethyladenosine cap (m6A(m)) in U6 small nuclear RNA (snRNA), N(1)-methyladenine from tRNAs and internal m6A in mRNAs. In the cytoplasm, mediates demethylation of m6A and m6A(m) in mRNAs and N(1)-methyladenine from tRNAs. " F1QGZ6,"PROTEIN NAMES: Maternal embryonic leucine zipper kinase (zMelk) (Protein kinase PK38) PROTEIN FAMILY: Protein kinase superfamily, CAMK Ser/Thr protein kinase family, SNF1 subfamily ORGANISM: Danio rerio (Zebrafish) (Brachydanio rerio) SIMILARITY: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily. FUNCTION: Serine/threonine-protein kinase involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation (By similarity). Also plays a role in primitive hematopoiesis, possibly by affecting the expression of genes critical for hematopoiesis. KEYWORDS: Apoptosis;ATP-binding;Cell cycle;Cell membrane;Kinase;Lipid-binding;Membrane;Nucleotide-binding;Phosphoprotein;Reference proteome;Serine/threonine-protein kinase;Transferase SUBCELLULAR LOCATION: Cell membrane ; Peripheral membrane protein " F1R777,"PROTEIN NAMES: N6-adenosine-methyltransferase subunit METTL3 (N6-adenosine-methyltransferase 70 kDa subunit) (MT-A70) PROTEIN FAMILY: MT-A70-like family ORGANISM: Danio rerio (Zebrafish) (Brachydanio rerio) SIMILARITY: Belongs to the MT-A70-like family. FUNCTION: The METTL3-METTL14 heterodimer forms a N6-methyltransferase complex that methylates adenosine residues at the N(6) position of some RNAs and regulates various processes such as the circadian clock, differentiation of embryonic and hematopoietic stem cells, cortical neurogenesis, response to DNA damage, differentiation of T-cells and primary miRNA processing. In the heterodimer formed with mettl14, mettl3 constitutes the catalytic core (By similarity). N6-methyladenosine (m6A), which takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs, plays a role in mRNA stability, processing and translation efficiency (By similarity). M6A is also involved in hematopoietic stem cells specification: m6A methylation and subsequent destabilization of mRNAs, such as notch1a, leads to decreased Notch signaling, promoting endothelial to hematopoietic transition. M6A also takes place in other RNA molecules, such as primary miRNA (pri-miRNAs) (By similarity). Mediates methylation of pri-miRNAs (By similarity). KEYWORDS: Cytoplasm;Differentiation;DNA damage;Methyltransferase;Nucleus;Reference proteome;RNA-binding;S-adenosyl-L-methionine;Spermatogenesis;Transferase SUBCELLULAR LOCATION: Nucleus Nucleus speckle Cytoplasm Note=Colocalizes with speckles in interphase nuclei. Suggesting that it may be associated with nuclear pre-mRNA splicing components. " F1RAX4,"PROTEIN NAMES: Receptor for retinol uptake stra6 (Stimulated by retinoic acid gene 6 protein homolog) ORGANISM: Danio rerio (Zebrafish) (Brachydanio rerio) FUNCTION: Retinol transporter. Accepts retinol from the extracellular retinol-binding protein rbp4, mediates retinol transport across the cell membrane, and then transmits retinol to the cytoplasmic retinol-binding protein rbp1. Required for normal vitamin A homeostasis. KEYWORDS: 3D-structure;Calmodulin-binding;Cell membrane;Membrane;Receptor;Reference proteome;Retinol-binding;Transmembrane;Transmembrane helix;Transport;Vitamin A SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein " F1RE08,"PROTEIN NAMES: Prostacyclin synthase (Hydroperoxy icosatetraenoate dehydratase) (Prostaglandin I2 synthase) (PGIS) PROTEIN FAMILY: Cytochrome P450 family ORGANISM: Danio rerio (Zebrafish) (Brachydanio rerio) SIMILARITY: Belongs to the cytochrome P450 family. FUNCTION: Catalyzes the isomerization of prostaglandin H2 to prostacyclin (= prostaglandin I2).; FUNCTION: Catalyzes the biosynthesis and metabolism of eicosanoids. Catalyzes the isomerization of prostaglandin H2 to prostacyclin (= prostaglandin I2), a potent mediator of vasodilation and inhibitor of platelet aggregation. Additionally, displays dehydratase activity, toward hydroperoxyeicosatetraenoates (HPETEs), especially toward (15S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoate (15(S)-HPETE) (By similarity). KEYWORDS: 3D-structure;Endoplasmic reticulum;Fatty acid biosynthesis;Fatty acid metabolism;Heme;Iron;Isomerase;Lipid biosynthesis;Lipid metabolism;Lyase;Membrane;Metal-binding;Prostaglandin biosynthesis;Prostaglandin metabolism;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Single-pass membrane protein " F1RE57,PROTEIN NAMES: Palmitoyltransferase ZDHHC3-A (Acyltransferase ZDHHC3A) (Zinc finger DHHC domain-containing protein 3) PROTEIN FAMILY: DHHC palmitoyltransferase family ORGANISM: Danio rerio (Zebrafish) (Brachydanio rerio) SIMILARITY: Belongs to the DHHC palmitoyltransferase family. FUNCTION: Golgi-localized palmitoyltransferase that catalyzes the addition of palmitate onto various protein substrates and regulates their association with membranes (Probable). Has no stringent fatty acid selectivity and in addition to palmitate can also transfer onto target proteins myristate from tetradecanoyl-CoA and stearate from octadecanoyl-CoA (Probable). KEYWORDS: Acyltransferase;Golgi apparatus;Lipoprotein;Membrane;Palmitate;Reference proteome;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus membrane ; Multi-pass membrane protein F4HU58,PROTEIN NAMES: tRNA-specific adenosine deaminase TAD1 (AtTAD1) (tRNA-specific adenosine-37 deaminase TAD1) PROTEIN FAMILY: ADAT1 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the ADAT1 family. FUNCTION: Involved in RNA editing. Catalyzes the specific deamination of adenosine-37 in the cytosolic tRNA-Ala. Generates inosine at the position 3'-adjacent to the anticodon tRNA-Ala. KEYWORDS: Hydrolase;Metal-binding;Nucleus;Reference proteome;tRNA processing;Zinc SUBCELLULAR LOCATION: Nucleus F4HVG8,"PROTEIN NAMES: Chloroplast sensor kinase, chloroplastic PROTEIN FAMILY: Chloroplast sensor kinase protein family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the chloroplast sensor kinase protein family. FUNCTION: Sensor kinase that senses the plastoquinone (PQ) redox state involved in stoichiometry adjustment of both photosystems (e.g. long-term adaptation via transcriptional regulation of reaction center genes of photosystems I and II) and state transitions (e.g. short-term adaptation involving reversible post-translational phosphorylation of light-harvesting complex II, LHC II), thus linking photosynthesis with gene expression in chloroplasts. Autophosphorylates, probably on a tyrosine residue. Probably phosphorylates SIGA/SIG1 in response to plastoquinone redox state modification. Reduced PQ suppresses its autophosphorylation activity. Represses expression of a number of chloroplast-encoded genes. KEYWORDS: 3Fe-4S;Alternative splicing;Chloroplast;Coiled coil;Iron;Iron-sulfur;Kinase;Metal-binding;Phosphoprotein;Plastid;Reference proteome;Transferase;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast stroma Note=Associates with thylakoid membranes. " F4HVJ3,"PROTEIN NAMES: Protein POLLEN DEFECTIVE IN GUIDANCE 1 PROTEIN FAMILY: TAPT1 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the TAPT1 family. FUNCTION: Probable component of the calreticulin 3 (CRT3) complex, acting probably as a co-chaperone involved in protein retention in the endoplasmic reticulum lumen. Required for micropylar pollen tube guidance. Plays an essential role in cell plate orientation or positioning in early embryo patterning. KEYWORDS: Endoplasmic reticulum;Membrane;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Multi-pass membrane protein Endoplasmic reticulum lumen Note=Exclusive ER lumen localization and no membrane localization in previous research. " F4HVW5,"PROTEIN NAMES: Protein VASCULAR ASSOCIATED DEATH 1, chloroplastic ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Involved in ethylene- and salicylic acid-dependent cell death control associated with cells in the vicinity of vascular bundles. KEYWORDS: Apoptosis;Chloroplast;Coiled coil;Ethylene signaling pathway;Glycoprotein;Hypersensitive response;Membrane;Plant defense;Plastid;Reference proteome;Transit peptide;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Single-pass membrane protein Plastid, chloroplast " F4HXY7,PROTEIN NAMES: CDP-diacylglycerol--serine O-phosphatidyltransferase 1 (Phosphatidylserine synthase 1) PROTEIN FAMILY: CDP-alcohol phosphatidyltransferase class-I family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the CDP-alcohol phosphatidyltransferase class-I family. FUNCTION: Catalyzes a base-exchange reaction in which the polar head group of phosphatidylethanolamine (PE) or phosphatidylcholine (PC) is replaced by L-serine. Is essential for phosphatidylserine (PS) biosynthesis and PE seems to be the most plausible substrate. Plays an important role in microspore maturation. PATHWAY: Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from CDP-diacylglycerol: step 1/2. KEYWORDS: Alternative splicing;Endoplasmic reticulum;Lipid biosynthesis;Lipid metabolism;Membrane;Nucleus;Phospholipid biosynthesis;Phospholipid metabolism;Reference proteome;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein Nucleus envelope Note=Mainly localized in nuclei and ER membranes during pollen development. F4HZG9,"PROTEIN NAMES: Intermediate cleaving peptidase 55, mitochondrial (AtICP55) (Protein INTERMEDIATE CLEAVAGE PEPTIDASE 55) PROTEIN FAMILY: Peptidase M24B family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the peptidase M24B family. FUNCTION: Aminopeptidase which cleaves preprotein intermediates that carry destabilizing N-ter amino acid residues after the mitochondrial processing peptidase (MPP) cleavage site and is thus critical for stabilization of the mitochondrial proteome. KEYWORDS: Alternative splicing;Aminopeptidase;Hydrolase;Manganese;Metal-binding;Metalloprotease;Mitochondrion;Nucleus;Protease;Reference proteome;Transit peptide SUBCELLULAR LOCATION: [Isoform 1]: Mitochondrion.; SUBCELLULAR LOCATION: [Isoform 2]: Nucleus " F4I2J8,PROTEIN NAMES: Splicing factor Cactin PROTEIN FAMILY: CACTIN family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the CACTIN family. FUNCTION: Plays a role in pre-mRNA splicing by facilitating excision of a subset of introns (By similarity). Required for embryogenesis. KEYWORDS: Alternative splicing;Coiled coil;mRNA processing;mRNA splicing;Nucleus;Phosphoprotein;Reference proteome;Spliceosome SUBCELLULAR LOCATION: Nucleus speckle F4I316,PROTEIN NAMES: SUN domain-containing protein 3 (AtSUN3) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Encodes a member of the mid-SUN subfamily of SUN-domain proteins that is localized to both the nuclear envelope and the ER. It is involved in early seed development and nuclear morphology. [TAIR]. KEYWORDS: Coiled coil;Endoplasmic reticulum;Membrane;Nucleus;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Nucleus membrane ; Multi-pass membrane protein Endoplasmic reticulum membrane ; Multi-pass membrane protein F4I443,"PROTEIN NAMES: BRCA1-associated RING domain protein 1 (AtBARD1) (Protein REPRESSOR OF WUSCHEL 1) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Binds specifically to H3K4me3 regions of target genes (e.g. WUS and WOX5) promoters to repress their transcription via chromatin remodeling. Required for the shoot apical meristem (SAM) organization and maintenance, by confining WUS expression to the organizing center, and for the quiescent center (QC) development in the root apical meristem (RAM), by repressing WOX5 expression in the root proximal meristem. Plays a role in DNA repair and in cell-cycle control. Required for the repair of DNA double-strand breaks (DSBs), both natural and induced by genotoxic stress, by homologous recombination (HR). KEYWORDS: Alternative splicing;Developmental protein;DNA damage;DNA repair;DNA-binding;Metal-binding;Nucleus;Reference proteome;Repeat;Repressor;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus " F4IB81,"PROTEIN NAMES: LysM domain receptor-like kinase 3 (LysM-containing receptor-like kinase 3) PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. FUNCTION: Putative Lysin motif (LysM) receptor kinase that may recognize microbe-derived N-acetylglucosamine (NAG)-containing ligands. KEYWORDS: ATP-binding;Cell membrane;Disulfide bond;Glycoprotein;Kinase;Membrane;Nucleotide-binding;Phosphoprotein;Receptor;Reference proteome;Repeat;Serine/threonine-protein kinase;Signal;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass membrane protein " F4ICB6,"PROTEIN NAMES: Protein IMPAIRED IN BABA-INDUCED STERILITY 1 PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. FUNCTION: Required for beta-aminobutyric acid (BABA)-induced resistance (BABA-IR) against bacteria (e.g. P.syringae) and oomycetes (e.g. H.parasitica) via priming for salicylate (SA)-dependent defense responses such as pathogenesis-related PR-1 gene expression and trailing necrosis. Involved in BABA-mediated sterility. Necessary for the inheritance of BABA-priming to next generation, especially for the primed to be primed phenotype which consists in an enhanced second BABA-priming in transgenerationally primed plants. KEYWORDS: Alternative splicing;ATP-binding;Kinase;Lipoprotein;Myristate;Nucleotide-binding;Plant defense;Reference proteome;Serine/threonine-protein kinase;Transferase " F4ICK8,PROTEIN NAMES: Protein IWS1 homolog 1 (AtIWS1) (Interacts with SPT6 protein 1) (Protein HIGH NITROGEN INSENSITIVE 9) (Protein SUPPRESSOR OF BES-1-D 1) PROTEIN FAMILY: IWS1 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the IWS1 family. FUNCTION: Transcription factor involved in RNA polymerase II (RNAPII) transcription regulation. Involved in transcription elongation. May function at post-recruitment and elongation steps of transcription. May be recruited by BZR2/BES1 to target genes and promote their expression during transcription elongation process. Required for brassinosteroid (BR)-induced gene expression. Required the for regulation of numerous nitrogen-responsive genes in roots. Acts in roots to repress NRT2.1 transcription in response to high nitrogen supply. This repression is associated with an IWS1-dependent increase of trimethylation on 'Lys-27' H3K27me3 at the NRT2.1 locus. KEYWORDS: Alternative splicing;Brassinosteroid signaling pathway;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus F4IDS7,"PROTEIN NAMES: Vacuolar sorting protein 18 PROTEIN FAMILY: VPS18 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the VPS18 family. FUNCTION: Essential protein required during embryogenesis. Believed to act as a core component of the putative HOPS endosomal tethering complex and of the class C core vacuole/endosome tethering (CORVET) complex. CORVET is required for vacuolar transport of SYP22. HOPS is required for the central vacuole formation. Involved in root development. Plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport pathways (By similarity). KEYWORDS: Coiled coil;Cytoplasm;Endosome;Membrane;Metal-binding;Protein transport;Reference proteome;Transport;Vacuole;Zinc;Zinc-finger SUBCELLULAR LOCATION: Endosome membrane ; Peripheral membrane protein ; Cytoplasmic side Vacuole membrane ; Peripheral membrane protein ; Cytoplasmic side Cytoplasm Note=Co-localizes with VPS39 at subdomains of the vacuolar membrane, and with VPS3 and RABF2B at punctate compartments in the cytoplasm, with faintly dispersed distribution in the cytosol. Sometimes observed at the vertex zone, the ring-shaped edge of vacuole-vacuole contact sites. Co-localizes with RABG3F. " F4IED2,"PROTEIN NAMES: NAC domain-containing protein 13 (ANAC013) (Protein NTM1-like 1) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Transcriptional activator activated by proteolytic cleavage through regulated intramembrane proteolysis (RIP). Involved in oxidative stress tolerance by mediating regulation of mitochondrial retrograde signaling during mitochondrial dysfunction. Interacts directly with the mitochondrial dysfunction DNA consensus motif 5'-CTTGNNNNNCA[AC]G-3', a cis-regulatory elements of several mitochondrial retrograde regulation-induced genes, and triggers increased oxidative stress tolerance. KEYWORDS: Activator;Alternative splicing;DNA-binding;Endoplasmic reticulum;Membrane;Nucleus;Reference proteome;Stress response;Transcription;Transcription regulation;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Single-pass membrane protein Nucleus Note=Localized primarily in endoplasmic reticulum membrane as dormant form and, upon oxidative stress, is processed into a transcriptionally active and nuclear form after a proteolytic cleavage through regulated intramembrane proteolysis (RIP). " F4IEM5,PROTEIN NAMES: LanC-like protein GCR2 (G-protein coupled receptor 2) PROTEIN FAMILY: LanC-like protein family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the LanC-like protein family. FUNCTION: May play a role in abscisic acid (ABA) signaling. KEYWORDS: 3D-structure;Abscisic acid signaling pathway;Metal-binding;Reference proteome;Zinc F4IGZ2,"PROTEIN NAMES: Protein SMAX1-LIKE 8 (AtSMXL8) (Protein D53-like 3) (AtD53-like 3) (Protein D53-like SMXL 8) PROTEIN FAMILY: ClpA/ClpB family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the ClpA/ClpB family. FUNCTION: Probable component of a transcriptional corepressor complex involved in branching control. Regulates cotyledon expansion and lateral root growth, but not germination or hypocotyl elongation. Promotes auxin transport and PIN1 accumulation in the stem and represses BRC1/TCP18 expression in axillary buds. KEYWORDS: Alternative splicing;Nucleus;Reference proteome;Repeat;Transcription;Transcription regulation;Ubl conjugation SUBCELLULAR LOCATION: Nucleus " F4IPK2,PROTEIN NAMES: Transcription initiation factor TFIID subunit 14 (Protein AF-9 homolog b) (TBP-associated factor 14) (AtTAF14) PROTEIN FAMILY: YAF9 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the YAF9 family. FUNCTION: Negative regulator of flowering controlling the H4K5 acetylation levels in the FLC and FT chromatin. Positively regulates FLC expression. Component of the transcription factor IID (TFIID) complex that is essential for mediating regulation of RNA polymerase transcription. Component of the SWR1 complex which mediates the ATP-dependent exchange of histone H2A for the H2A variant HZT1 leading to transcriptional regulation of selected genes by chromatin remodeling. Component of a NuA4 histone acetyltransferase complex which is involved in transcriptional activation of selected genes principally by acetylation of nucleosomal histones H4 and H2A. KEYWORDS: Activator;Alternative splicing;Coiled coil;Cytoplasm;Developmental protein;Differentiation;Flowering;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Cytoplasm Nucleus F4IRB4,"PROTEIN NAMES: Transcription factor MYB88 (Myb-related protein 88) (AtMYB88) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Transcription factor that binds to DNA in promoters cis-regulatory element 5'-GGCGCGC-3' of cell cycle genes, including cyclins, cyclin-dependent kinases (CDKs), and components of the pre-replication complex. Binds to DNA in promoters cis-regulatory element 5'-AGCCG-3' of auxin regulated genes (e.g. PIN3 and PIN7). Together with FAMA and MYB124, ensures that stomata contain just two guard cells (GCs) by enforcing a single symmetric precursor cell division before stomatal maturity. Represses the expression of the mitosis-inducing factors CDKB1-1 and CDKA-1, specifically required for the last guard mother cells (GMC) symmetric divisions in the stomatal pathway. Represses CYCA2-3 in newly formed guard cells. Together with MYB88, regulates stomata spacing by restricting divisions late in the stomatal cell lineage thus limiting the number of GMC divisions. In collaboration with CDKB1-1 and CDKB1-2, restrict the G1/S transition and chloroplast and nuclear number during stomatal formation, and normally maintain fate and developmental progression throughout the stomatal cell lineage. Involved in sensing and/or transducing abiotic stress (e.g. drought and salt), probably via the positive regulation of NAC019. Regulates female reproduction being required for entry into megasporogenesis, probably via the regulation of cell cycle genes. Plays a minor role in lateral roots (LRs) initiation. Involved complementarily in establishing the gravitropic set-point angles of lateral roots by regulating the transcription of PIN3 and PIN7 in gravity-sensing cells of primary and lateral roots. KEYWORDS: Alternative splicing;Cell cycle;Developmental protein;DNA-binding;Nucleus;Reference proteome;Repeat;Repressor;Stress response;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " F4IS56,"PROTEIN NAMES: Integrin-linked protein kinase 1 (Ankyrin protein kinase 1) PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. FUNCTION: Functions as a link between plant defense pathways, stress responses and potassium homeostasis. Promotes osmotic stress sensitivity, responses to the bacterial-derived pathogen-associated molecular pattern (PAMP) flg22, and resistance to bacterial pathogens. Promotes the accumulation of POT5/HAK5, a potassium transporter that mediates high-affinity uptake during potassium deficiency. KEYWORDS: Alternative splicing;ANK repeat;ATP-binding;Cell membrane;Endoplasmic reticulum;Kinase;Membrane;Nucleotide-binding;Phosphoprotein;Plant defense;Reference proteome;Repeat;Serine/threonine-protein kinase;Stress response;Transferase SUBCELLULAR LOCATION: Cell membrane ; Peripheral membrane protein Endoplasmic reticulum membrane ; Peripheral membrane protein " F4IY62,"PROTEIN NAMES: UTP--glucose-1-phosphate uridylyltransferase 3, chloroplastic (UDP-glucose pyrophosphorylase 3) PROTEIN FAMILY: UDPGP type 1 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the UDPGP type 1 family. FUNCTION: Involved in the biosynthesis of sulfolipids in the chloroplast. Catalyzes the first committed step in sulfolipid biosynthesis. Converts glucose 1-phosphate to UDP-glucose, the precursor of the polar head of sulfolipid. In addition to glucose 1-phosphate, can use galactose 1-phosphate, but with much lower activity. No uridyltransferase activity with other hexose monophosphates. Specific for UTP and cannot use ATP, CTP, and GTP. KEYWORDS: Chloroplast;Nucleotidyltransferase;Plastid;Reference proteome;Transferase;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast " F4J117,"PROTEIN NAMES: Phosphoglucan phosphatase LSF1, chloroplastic (Phosphoglucan phosphatase like sex Four1) (Protein LIKE SEX4 1) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Starch granule-associated phosphoglucan phosphatase involved in the control of starch accumulation. Participates in the regulation of the initial steps of starch degradation at the granule surface. May release a different set of phosphate groups from those removed by DSP4. KEYWORDS: 3D-structure;Carbohydrate metabolism;Chloroplast;Hydrolase;Plastid;Protein phosphatase;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast Note=Located on the surface of starch granules. " F4J264,"PROTEIN NAMES: Peptide chain release factor PrfB3, chloroplastic (AtPrfB3) PROTEIN FAMILY: Prokaryotic/mitochondrial release factor family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the prokaryotic/mitochondrial release factor family. FUNCTION: Involved in the light- and stress-dependent regulation of stability of 3' processed petB transcripts, thus regulating cytochrome b6 accumulation, a rate-limiting step in photosynthetic electron transport. May be recruited to specifically protect petB transcripts against 3'-5' exonucleolytic attack by masking the 3' ends. Does not function as release factor. KEYWORDS: Chloroplast;Growth regulation;Plastid;Reference proteome;RNA-binding;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast stroma Plastid, chloroplast " F4J2K2,"PROTEIN NAMES: Folylpolyglutamate synthase (DHFS-FPGS homolog C) (Folylpoly-gamma-glutamate synthetase) (FPGS) (Tetrahydrofolylpolyglutamate synthase) (Tetrahydrofolate synthase) PROTEIN FAMILY: Folylpolyglutamate synthase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the folylpolyglutamate synthase family. FUNCTION: Catalyzes conversion of folates to polyglutamate derivatives allowing concentration of folate compounds in the cell and the intracellular retention of these cofactors, which are important substrates for most of the folate-dependent enzymes that are involved in one-carbon transfer reactions involved in purine, pyrimidine and amino acid synthesis. Essential for organellar and whole-plant folate homeostasis. PATHWAY: Cofactor biosynthesis; tetrahydrofolylpolyglutamate biosynthesis. KEYWORDS: ATP-binding;Ligase;Magnesium;Membrane;Metal-binding;Mitochondrion;Mitochondrion inner membrane;Nucleotide-binding;One-carbon metabolism;Reference proteome SUBCELLULAR LOCATION: Mitochondrion inner membrane Mitochondrion matrix " F4JCB2,"PROTEIN NAMES: RNA polymerase II C-terminal domain phosphatase-like 5 (FCP-like 5) (Carboxyl-terminal phosphatase-like 5) (AtCPL5) (CTD phosphatase-like 5) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Mediates the dephosphorylation of 'Ser-2' of the heptad repeats YSPTSPS in the C-terminal domain of the largest RNA polymerase II subunit (RPB1). This promotes the activity of RNA polymerase II. Positively regulates abscisic acid (ABA) and drought responses, including the regulation of specific genes expression. KEYWORDS: Abscisic acid signaling pathway;Activator;Hydrolase;Nucleus;Reference proteome;Stress response;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " F4JCX9,"PROTEIN NAMES: Protein LNK2 (Night light-inducible and clock-regulated 2) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Transcriptional coactivator necessary for expression of the clock genes PRR5 and TOC1. Antagonizes REV8 function in the regulation of anthocyanin accumulation. Involved in red light input to the clock. Activates clock-controlled genes with afternoon peak. Mediates light inhibition of hypocotyl elongation. Unable to bind to DNA, but recruited to the evening element (EE)-containing region of the PRR5 and TOC1 promoters through its interaction with the DNA binding proteins REV8 and REV4. KEYWORDS: Activator;Alternative splicing;Biological rhythms;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " F4JN35,"PROTEIN NAMES: Protein NTM1-like 9 (Calmodulin-binding NAC protein) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Transcriptional activator activated by proteolytic cleavage through regulated intramembrane proteolysis (RIP). Calmodulin-regulated transcriptional repressor. Binds several synthetic promoters with randomly selected binding sites. Functions synergistically with SNI1 as negative regulator of pathogen-induced PR1 expression and basal resistance to a virulent strain of P.syringae. Binds directly to the promoter of the PR1 gene. Acts as a positive regulator of innate immunity. Involved in the effector-triggered immunity (ETI) induction of immunity-related gene expression. Mediates osmotic stress signaling in leaf senescence by up-regulating senescence-associated genes. KEYWORDS: Activator;Alternative splicing;Calmodulin-binding;Cell membrane;DNA-binding;Endoplasmic reticulum;Membrane;Nucleus;Reference proteome;Repressor;Stress response;Transcription;Transcription regulation;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass membrane protein Endoplasmic reticulum membrane ; Single-pass membrane protein Nucleus Note=Localized primarily in plasma membrane or endoplasmic reticulum membrane as dormant form and, upon osmotic stress or pathogen attack, is processed into a transcriptionally active and nuclear form after a proteolytic cleavage through regulated intramembrane proteolysis (RIP). " F4JQH3,"PROTEIN NAMES: Aminopeptidase P1 (AtAPP1) PROTEIN FAMILY: Peptidase M24B family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the peptidase M24B family. FUNCTION: Catalyzes the removal of a penultimate prolyl residue from the N-termini of peptides, such as Arg-Pro-Pro (By similarity). Aminopeptidase that binds to the auxin transport inhibitor N-1-naphthylphthalamic acid (NPA). May play a negative role in the regulation of PIN auxin transport proteins. KEYWORDS: Acetylation;Alternative splicing;Aminopeptidase;Cell membrane;Cytoplasm;Direct protein sequencing;Endoplasmic reticulum;Glycoprotein;Hydrolase;Manganese;Membrane;Metal-binding;Metalloprotease;Microsome;Protease;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Cell membrane ; Peripheral membrane protein Microsome membrane ; Peripheral membrane protein " F4JRB0,"PROTEIN NAMES: Transcription factor HHO5 (MYB-domain transcription factor HHO5) (Protein HRS1 HOMOLOG 5) (Protein ULT1 INTERACTING FACTOR 1) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Transcriptional repressor that functions with ULT1 in a pathway which regulates floral meristem homeostasis and organ number in the flower. Binds specifically to the DNA sequence motif 5'-GTAGATTCCT-3' of WUS promoter, and may be involved in direct regulation of WUS expression. Binds specifically to the DNA sequence motif 5'-AAGAATCTTT-3' found in the promoters of AG and the NAC domain genes CUC1, CUC2 and CUC3, and may be involved in direct regulation of these gene expressions. KEYWORDS: Alternative splicing;Cytoplasm;DNA-binding;Flowering;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus Cytoplasm, cytosol Note=Localizes in cytosolic foci. " F4JTE7,"PROTEIN NAMES: (DL)-glycerol-3-phosphatase 1, mitochondrial (5-amino-6-(5-phospho-D-ribitylamino)uracil phosphatase) (AtGpp1/PyrP3) (5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate phosphatase) (ARPP phosphatase) (Glycerol-1-phosphatase 1) (AtGPP1) (Haloacid dehalogenase-like hydrolase domain-containing protein GPP1) PROTEIN FAMILY: HAD-like hydrolase superfamily, DOG/GPP family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the HAD-like hydrolase superfamily. DOG/GPP family. FUNCTION: Acts as a glycerol-3-phosphatase with higher stereospecificity for L-glycerol-3-phosphate than DL-glycerol-3-phosphate. Can also dephosphorylate in vitro 5-amino-6-(5-phospho-D-ribitylamino)uracil, also known as ARPP. KEYWORDS: Hydrolase;Magnesium;Metal-binding;Mitochondrion;Reference proteome;Riboflavin biosynthesis;Transit peptide SUBCELLULAR LOCATION: Mitochondrion " F4JYE9,"PROTEIN NAMES: Dihydrofolate synthetase (AtDFA) (Protein GLOBULAR ARREST 1) PROTEIN FAMILY: Folylpolyglutamate synthase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the folylpolyglutamate synthase family. FUNCTION: Conversion of folates to polyglutamate derivatives, including tetrahydrofolate. Required during embryogenesis; from maternal tissues until the globular stage, and from the embryo after the globular stage. PATHWAY: Cofactor biosynthesis; tetrahydrofolate biosynthesis; 7,8-dihydrofolate from folate: step 1/1. KEYWORDS: ATP-binding;Ligase;Magnesium;Metal-binding;Mitochondrion;Nucleotide-binding;One-carbon metabolism;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Mitochondrion matrix " F4JZN6,"PROTEIN NAMES: 3-dehydrosphinganine reductase TSC10B (3-ketodihydrosphingosine reductase) (KDS reductase) (3-ketosphinganine reductase) PROTEIN FAMILY: Short-chain dehydrogenases/reductases (SDR) family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FUNCTION: Catalyzes the reduction of 3'-oxosphinganine (3-ketodihydrosphingosine/KDS) to sphinganine (dihydrosphingosine/DHS), the second step of de novo sphingolipid biosynthesis. In plants, sphingolipids seems to play a critical role in mineral ion homeostasis, most likely through their involvement in the ion transport functionalities of membrane systems in the root. Is stereospecific for D-erythro-DHS production and does not produce L-threo-DHS. PATHWAY: Lipid metabolism; sphingolipid metabolism. KEYWORDS: Endoplasmic reticulum;Lipid metabolism;Membrane;NADP;Nucleotide-binding;Oxidoreductase;Reference proteome;Sphingolipid metabolism;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein " F4K5X6,"PROTEIN NAMES: Protein REVEILLE 2 (MYB family transcription factor Circadian 1) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Positive regulator for cold-responsive gene expression and cold tolerance. Part of a regulatory feedback loop that controls a subset of the circadian outputs and modulates the central oscillator. Negatively self-regulates its own expression. KEYWORDS: Biological rhythms;DNA-binding;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: Regulated at the level of mRNA maturation by RH42. The RVE2 pre-mRNA can be alternatively spliced, generating a poison cassette exon that harbors an very early in-frame premature termination codon. The resulting severely truncated mRNA is not efficiently translated." F4K933,"PROTEIN NAMES: Protein EFFECTOR OF TRANSCRIPTION 2 (AtET2) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Transcriptional regulator involved in the regulation of cell differentiation in meristems. Probably regulates the expression of various KNAT genes involved in the maintenance of the cells in an undifferentiated, merismastic state. Plays a role in the regulation of gibberellin 20 oxidase and the gibberellin-regulated protein GASA4. Localizes in the nucleus during the cellular differentiation state and may act via a single strand cutting domain. Transcriptional regulator required for the induction of dormancy during late seed development. Interacts genetically with FUS3 and may be component of the same regulatory pathway during embryogenesis. Binds both linear and supercoiled DNA without sequence preference. KEYWORDS: Cytoplasm;DNA-binding;Nucleus;Reference proteome;Repeat;Transcription;Transcription regulation SUBCELLULAR LOCATION: Cytoplasm Nucleus Note=Localizes to the cytoplasm in non-differentiating cells and to the nucleus in differentiating cells. " F4KAF2,"PROTEIN NAMES: Protein MICRORCHIDIA 4 (AtMORC4) (Protein CRT1-homolog 4) (CRT1-h4) PROTEIN FAMILY: MORC ATPase protein family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the MORC ATPase protein family. FUNCTION: Exhibits ATPase activity. Binds DNA/RNA in a non-specific manner and exhibits endonuclease activity. Probably involved in DNA repair. Involved in RNA-directed DNA methylation (RdDM) as a component of the RdDM machinery and required for gene silencing. May also be involved in the regulation of chromatin architecture to maintain gene silencing. Together with MORC7, acts to suppress a wide set of non-methylated protein-coding genes, especially involved in pathogen response. Positive regulator of defense against the oomycete Hyaloperonospora arabidopsidis (Hpa). KEYWORDS: ATP-binding;Chromatin regulator;Coiled coil;DNA damage;DNA repair;DNA-binding;Endonuclease;Hydrolase;Kinase;Nuclease;Nucleotide-binding;Nucleus;Plant defense;Reference proteome;RNA-binding;RNA-mediated gene silencing;Transferase SUBCELLULAR LOCATION: Nucleus Note=Accumulates in discrete nuclear bodies adjacent to chromocenters. " F4KB17,"PROTEIN NAMES: Katanin p80 WD40 repeat-containing subunit B1 homolog KTN80.3 PROTEIN FAMILY: WD repeat KATNB1 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the WD repeat KATNB1 family. FUNCTION: May participate in a complex which severs microtubules in an ATP-dependent manner (By similarity). Microtubule severing may promote rapid reorganization of cellular microtubule arrays (By similarity). Confers precision to microtubule (MT) severing by specific targeting of KTN1 to MT cleavage sites such as crossover or branching nucleation sites. Together with other KTN80s, regulates cell elongation by modulating MT organization. KEYWORDS: Cytoplasm;Cytoskeleton;Microtubule;Reference proteome;Repeat;WD repeat SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton Note=Present in dynamic discrete particles specifically localized to microtubule (MT) crossovers and branching nucleation sites. " F4KCE9,"PROTEIN NAMES: Kinetochore-associated protein KNL-2 homolog (Protein KINETOCHORE NULL 2) PROTEIN FAMILY: KNL2 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the KNL2 family. FUNCTION: Involved in recognition of centromeres and centromeric localization of the centromere-specific histone CENH3. Required for normal progression of mitosis and meiosis. May play a role in the determination of the epigenetic status of centromeres. Binds DNA and RNA in vitro. KEYWORDS: Cell cycle;Cell division;Centromere;Chromosome;DNA-binding;Meiosis;Mitosis;Nucleus;Reference proteome;RNA-binding SUBCELLULAR LOCATION: Nucleus, nucleoplasm Nucleus, nuclear body Nucleus, nucleolus Chromosome, centromere Note=Localizes at centromeres during all stages of the mitotic cell cycle, except from metaphase to mid-anaphase. Colocalizes with CENH3 at centromeres in interphase nuclei. " F4KDA5,"PROTEIN NAMES: Mitochondrial intermediate peptidase, mitochondrial (AtOCT1) PROTEIN FAMILY: Peptidase M3 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the peptidase M3 family. FUNCTION: Aminopeptidase which cleaves preproteins, imported into the mitochondrion, to their mature size. Could cleave both preproteins and preprotein intermediates already cleaved by the mitochondrial processing peptidase (MPP). KEYWORDS: Hydrolase;Metal-binding;Metalloprotease;Mitochondrion;Protease;Reference proteome;Transit peptide;Zinc SUBCELLULAR LOCATION: Mitochondrion " F4KFS7,"PROTEIN NAMES: Membrane protein of ER body 2 PROTEIN FAMILY: CCC1 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the CCC1 family. FUNCTION: May sequester excess cytosolic iron and manganese into endoplasmic reticulum to reduce metal ion toxicity. Not essential for the accumulation of ER body components, including PYK10. KEYWORDS: Alternative splicing;Coiled coil;Endoplasmic reticulum;Membrane;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein Note=Located in ER bodies. " F4KH86,"PROTEIN NAMES: tRNA-specific adenosine deaminase TAD3 (AtTAD3) (Protein EMBRYO DEFECTIVE 2820) (tRNA-specific adenosine-34 deaminase TAD3) PROTEIN FAMILY: Cytidine and deoxycytidylate deaminase family, ADAT3 subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the cytidine and deoxycytidylate deaminase family. ADAT3 subfamily. FUNCTION: Involved in RNA editing. Catalyzes the specific deamination of adenosine-34 in several cytosolic tRNA species. Generates inosine at the wobble position of the anticodon loop. KEYWORDS: Cytoplasm;Hydrolase;Metal-binding;Nucleus;Reference proteome;tRNA processing;Zinc SUBCELLULAR LOCATION: Nucleus Cytoplasm Note=Localizes predominantly to the nucleus. " F5BHA2,"PROTEIN NAMES: Tabersonine/lochnericine 19-hydroxylase (Cytochrome P450 71BJ1) PROTEIN FAMILY: Cytochrome P450 family ORGANISM: Catharanthus roseus (Madagascar periwinkle) (Vinca rosea) SIMILARITY: Belongs to the cytochrome P450 family. FUNCTION: Component of the monoterpenoid indole alkaloids (MIAs, e.g. echitovenine, tabersonine, lochnericine, 19-hydroxytabersonine and horhammericine) biosynthetic pathway; MIAs are used in cancer treatment and other medical applications. Cytochrome P450 catalyzing the conversion of (-)-tabersonine to 19-hydroxytabersonine, of lochnericine to horhammericine and of (-)-vincadifformine to (-)-minovincinine. PATHWAY: Alkaloid biosynthesis. KEYWORDS: Alkaloid metabolism;Endoplasmic reticulum;Glycoprotein;Heme;Iron;Lyase;Membrane;Metal-binding;Monooxygenase;Oxidoreductase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Single-pass membrane protein " F5GUE5,"PROTEIN NAMES: Smad-related protein daf-14 (Abnormal dauer formation protein 14) ORGANISM: Caenorhabditis elegans FUNCTION: Probably an atypical receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta-like daf-7 signaling. Plays a role in TGF-beta-like daf-7 signaling in regulating entry into a developmentally arrested larval state known as dauer, in response to harsh environmental conditions; partially redundant with R-SMAD daf-8. KEYWORDS: Alternative splicing;Reference proteome;Transcription;Transcription regulation " F5HKX0,"PROTEIN NAMES: CLIP domain-containing serine protease B9 (Phenoloxidase-activating enzyme B9) [Cleaved into: CLIP domain-containing serine protease B9 light chain; CLIP domain-containing serine protease B9 heavy chain] PROTEIN FAMILY: Peptidase S1 family, CLIP subfamily ORGANISM: Anopheles gambiae (African malaria mosquito) SIMILARITY: Belongs to the peptidase S1 family. CLIP subfamily. FUNCTION: Serine protease that functions in the melanization-mediated immune response. Cleaves and activates prophenoloxidase (PPO), which is required for the activation of the prophenoloxidase cascade probably following the recognition of pathogen-derived products. KEYWORDS: Disulfide bond;Glycoprotein;Hydrolase;Immunity;Innate immunity;Protease;Reference proteome;Secreted;Serine protease;Signal;Zymogen SUBCELLULAR LOCATION: Secreted " F8VQN3,"PROTEIN NAMES: 12-(S)-hydroxy-5,8,10,14-eicosatetraenoic acid receptor (12-(S)-HETE receptor) (12-HETER) (G-protein coupled receptor 31) PROTEIN FAMILY: G-protein coupled receptor 1 family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the G-protein coupled receptor 1 family. FUNCTION: High-affinity receptor for 12-(S)-hydroxy-5,8,10,14-eicosatetraenoic acid (12-S-HETE), with much lower affinities for other HETE isomers (By similarity). 12-S-HETE is a eicosanoid, a 12-lipoxygenase (ALOX12) metabolite of arachidonic acid, involved in many physiologic and pathologic processes, such as cell growth, adhesion, inflammation and cancer promotion. 12-S-HETE-binding leads to activation of ERK1/2 (MAPK3/MAPK1), MEK, and NF-kappa-B pathways and leads to cell growth. Plays a crucial role for proliferation, survival and macropinocytosis of KRAS-dependent cancer cells by mediating the translocation of KRAS from the endoplasmic reticulum to the plasma membrane (PM) and its association with the PM (By similarity). Contributes to enhanced immune responses by inducing dendrite protrusion of small intestinal CX3CR1(+) phagocytes for the uptake of luminal antigens. Acts also as a key receptor for 12-(S)-HETE-mediated liver ischemia reperfusion injury.; FUNCTION: Proton-sensing G protein-coupled receptor. KEYWORDS: Cell membrane;G-protein coupled receptor;Glycoprotein;Membrane;Receptor;Reference proteome;Transducer;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein " F9UPU7,"PROTEIN NAMES: Probable ketoamine kinase lp_1983 PROTEIN FAMILY: Fructosamine kinase family ORGANISM: Lactiplantibacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1) (Lactobacillus plantarum) SIMILARITY: Belongs to the fructosamine kinase family. FUNCTION: Ketoamine kinase that phosphorylates ketoamines, such as erythruloselysine, erythrulosecadaverine, ribuloselysine and ribulosecadaverine, on the third carbon of the sugar moiety to generate ketoamine 3-phosphate. Has higher activity on free lysine (erythruloselysine and ribuloselysine), than on ribuloselysine and erythruloselysine residues on glycated proteins. KEYWORDS: ATP-binding;Kinase;Nucleotide-binding;Reference proteome;Transferase " F9VN79,"PROTEIN NAMES: Ribonuclease HI (RNase HI) (Sto-RNase HI) ORGANISM: Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) FUNCTION: Nuclease that specifically degrades the RNA of RNA-DNA hybrids. Endonucleolytically removes RNA primers from the Okazaki fragments of lagging strand synthesis on its own. In the presence of Mn(2+) or Co(2+) can also cleave an RNA-RNA hybrid; the dsRNase activity is 10- 100-fold lower than RNase H activity. Complements the temperature-sensitive phenotype of an E.coli double rnhA/rnhB (RNase H) disruption mutant. KEYWORDS: 3D-structure;Cytoplasm;Disulfide bond;DNA-binding;Endonuclease;Hydrolase;Magnesium;Manganese;Metal-binding;Nuclease;Reference proteome;RNA-binding SUBCELLULAR LOCATION: Cytoplasm MISCELLANEOUS: The protein is hyperthermostable, melting temperature (TM) for wild-type is 102 degrees Celsius, for the double Cys mutant is 93 degrees Celsius and for a C-terminal deletion of 6 residues is 77 degrees Celsius." F9W301,"PROTEIN NAMES: Kinesin-like protein KIN-1 (Kinesin-1-like protein PSS1) (Pollen semi-sterility protein 1) PROTEIN FAMILY: TRAFAC class myosin-kinesin ATPase superfamily, Kinesin family, KIN-1 subfamily ORGANISM: Oryza sativa subsp. japonica (Rice) SIMILARITY: Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. KIN-1 subfamily. FUNCTION: Kinesin-like motor protein that exhibits microtubule-stimulated ATPase activity. Plays an essential role in male meiotic chromosomal dynamics, male gametogenesis and anther dehiscence. May play a minor and nonessential role in regulating meiotic spindle formation. KEYWORDS: Alternative splicing;ATP-binding;Coiled coil;Cytoplasm;Microtubule;Motor protein;Nucleotide-binding;Reference proteome SUBCELLULAR LOCATION: Cytoplasm " G0L322,"PROTEIN NAMES: Beta-agarase A [Cleaved into: Beta-agarase A catalytic chain (AgaAc)] PROTEIN FAMILY: Glycosyl hydrolase 16 family ORGANISM: Zobellia galactanivorans (strain DSM 12802 / CCUG 47099 / CIP 106680 / NCIMB 13871 / Dsij) SIMILARITY: Belongs to the glycosyl hydrolase 16 family. FUNCTION: Cleaves the beta-1,4-linkages between beta-D-galactose and alpha-L-3,6-anhydro-galactose residues in agarose. Cleaves agarose in a random manner with retention of the anomeric-bond configuration, producing beta-anomers that give rise progressively to alpha-anomers when mutarotation takes place. KEYWORDS: 3D-structure;Direct protein sequencing;Glycosidase;Hydrolase;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted " G0S3J5,"PROTEIN NAMES: Serine/threonine-protein kinase RIO1 PROTEIN FAMILY: Protein kinase superfamily, RIO-type Ser/Thr kinase family ORGANISM: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (Thermochaetoides thermophila) SIMILARITY: Belongs to the protein kinase superfamily. RIO-type Ser/Thr kinase family. FUNCTION: Involved in the final steps of cytoplasmic maturation of the 40S ribosomal subunit (By similarity). In vitro, has strong ATPase activity and only low protein kinase activity. KEYWORDS: ATP-binding;Cytoplasm;Hydrolase;Kinase;Magnesium;Metal-binding;Nucleotide-binding;Reference proteome;Ribosome biogenesis;Serine/threonine-protein kinase;Transferase SUBCELLULAR LOCATION: Cytoplasm " G1UBD1,"PROTEIN NAMES: Particulate methane monooxygenase alpha subunit (Methane monooxygenase B subunit) (Particulate methane monooxygenase 45 kDa subunit) (Particulate methane monooxygenase 47 kDa subunit) (Particulate methane monooxygenase hydroxylase 45 kDa subunit) (Particulate methane monooxygenase hydroxylase alpha subunit) (pMMO-H alpha subunit) ORGANISM: Methylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath) FUNCTION: Methane monooxygenase is responsible for the initial oxygenation of methane to methanol in methanotrophs. At least in vitro, specific quinols can replace NADH as reductants. KEYWORDS: 3D-structure;Copper;Direct protein sequencing;Membrane;Metal-binding;Monooxygenase;NAD;Oxidoreductase;Reference proteome;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Multi-pass membrane protein Note=Located in intracellular membranes. " G2IQQ5,"PROTEIN NAMES: 2-keto-4-carboxy-3-hexenedioate hydratase (KCH hydratase) PROTEIN FAMILY: Metallo-dependent hydrolases superfamily ORGANISM: Sphingobium sp. (strain NBRC 103272 / SYK-6) SIMILARITY: Belongs to the metallo-dependent hydrolases superfamily. FUNCTION: Contributes to the degradation of lignin at the level of the protocatechuate 4,5-cleavage pathway. Catalyzes the hydration of the double bond of (3Z)-2-keto-4-carboxy-3-hexenedioate (KCH) to (4S)-4-carboxy-4-hydroxy-2-oxoadipate (CHA, also named (2S)-2-hydroxy-4-oxobutane-1,2,4-tricarboxylate). Is involved in the catabolism of both vanillate and syringate. PATHWAY: Secondary metabolite metabolism; lignin degradation. KEYWORDS: 3D-structure;Aromatic hydrocarbons catabolism;Lyase;Metal-binding;Reference proteome;Zinc " G2QFD0,"PROTEIN NAMES: Laccase-like multicopper oxidase 1 (LMCO) PROTEIN FAMILY: Multicopper oxidase family ORGANISM: Thermothelomyces thermophilus (strain ATCC 42464 / BCRC 31852 / DSM 1799) (Sporotrichum thermophile) SIMILARITY: Belongs to the multicopper oxidase family. FUNCTION: Yellow laccase-like multicopper oxidase that is able to oxidize a variety of phenolic compounds including standard laccase substrates such as 2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and 2,6-dimethoxyphenol (2,6-DMP). The existence of an ortho-hydroxy group is crucial for oxidation since pyrogallol and catechol, which contain ortho-hydroxy groups, are readily oxidized, which is not the case for resorcinol and hydroquinone, that contain meta- and para-hydroxy groups, respectively. The same is also true for the existence of a methoxy group in an ortho-position, since 2,6-DMP, guaiacol and ferulic and caffeic acids are also rather easily oxidized compared with the corresponding unsubstituted compound. Can be used for the bioconversion of 2',3,4-trihy-droxychalcone to 3',4'-dihydroxy-aurone, a bioactive aurone recently shown to possess inhibitory activity against several isoforms of the histone deacetylase complex (HDAC). KEYWORDS: 3D-structure;Copper;Disulfide bond;Glycoprotein;Metal-binding;Oxidoreductase;Reference proteome;Repeat;Signal " G2QG48,"PROTEIN NAMES: Xylooligosaccharide oxidase (XOS) PROTEIN FAMILY: Oxygen-dependent FAD-linked oxidoreductase family ORGANISM: Thermothelomyces thermophilus (strain ATCC 42464 / BCRC 31852 / DSM 1799) (Sporotrichum thermophile) SIMILARITY: Belongs to the oxygen-dependent FAD-linked oxidoreductase family. FUNCTION: Catalyzes the selective oxidation of C1 hydroxyl moieties on mono-, oligo- and polysaccharides with concomitant reduction of molecular oxygen to hydrogen peroxide. This results in the formation of the corresponding lactones, which typically undergo spontaneous hydrolysis. Xylooligosaccharide oxidase is able to oxidize a variety of substrates including D-xylose, D-cellobiose, lactose and arabinose. The enzyme acts primarily on xylooligosaccharides, indicating that it prefers pentose-based oligosaccharides over hexose-based oligosaccharides. KEYWORDS: 3D-structure;Disulfide bond;FAD;Flavoprotein;Glycoprotein;Nucleotide-binding;Oxidoreductase;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted " G2X4M1,PROTEIN NAMES: Secreted isochorismatase effector Isc1 PROTEIN FAMILY: Isochorismatase family ORGANISM: Verticillium dahliae (strain VdLs.17 / ATCC MYA-4575 / FGSC 10137) (Verticillium wilt) SIMILARITY: Belongs to the isochorismatase family. FUNCTION: Secreted isochorismatase required for full virulence of V.dahliae. Suppresses salicylate-mediated innate immunity of the host by disrupting the plant salicylate metabolism pathway via hydrolysis of its isochorismate precursor. KEYWORDS: Host cytoplasm;Host nucleus;Hydrolase;Reference proteome;Secreted;Virulence SUBCELLULAR LOCATION: Secreted Host cytoplasm Host nucleus Note=Lacks a signal peptide and uses an unconventional secretion pathway for delivering effectors which plays an important role in host-pathogen interactions. G3FNQ9,"PROTEIN NAMES: Cuticle-degrading serine protease (Neutral serine protease Aoz1) (Aoz) (PII) PROTEIN FAMILY: Peptidase S8 family ORGANISM: Orbilia oligospora (Nematode-trapping fungus) (Arthrobotrys oligospora) SIMILARITY: Belongs to the peptidase S8 family. FUNCTION: Hydrolyzes gelatin, casein, the chromogenic substrate azocoll and the cuticle of the nematode P.redivivus. Immobilizes P.redivivus. KEYWORDS: Collagen degradation;Direct protein sequencing;Glycoprotein;Hydrolase;Protease;Secreted;Serine protease;Signal;Zymogen SUBCELLULAR LOCATION: Secreted " G3V6U9,"PROTEIN NAMES: Actin-histidine N-methyltransferase (Protein-L-histidine N-tele-methyltransferase) (SET domain-containing protein 3) PROTEIN FAMILY: Class V-like SAM-binding methyltransferase superfamily, SETD3 actin-histidine methyltransferase family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the class V-like SAM-binding methyltransferase superfamily. SETD3 actin-histidine methyltransferase family. FUNCTION: Protein-histidine N-methyltransferase that specifically mediates 3-methylhistidine (tele-methylhistidine) methylation of actin at 'His-73'. Histidine methylation of actin is required for smooth muscle contraction of the laboring uterus during delivery (By similarity). Does not have protein-lysine N-methyltransferase activity and probably only catalyzes histidine methylation of actin (By similarity). KEYWORDS: Actin-binding;Cytoplasm;Luminescence;Methyltransferase;Nucleus;Phosphoprotein;Photoprotein;Reference proteome;S-adenosyl-L-methionine;Transferase;Ubl conjugation SUBCELLULAR LOCATION: Cytoplasm Nucleus Note=Localizes mainly in the cytoplasm. " G3V9M2,PROTEIN NAMES: C-Jun-amino-terminal kinase-interacting protein 2 (JIP-2) (JNK-interacting protein 2) (Islet-brain-2) (IB-2) (JNK MAP kinase scaffold protein 2) (Mitogen-activated protein kinase 8-interacting protein 2) PROTEIN FAMILY: JIP scaffold family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the JIP scaffold family. FUNCTION: The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. JIP2 inhibits IL1 beta-induced apoptosis in insulin-secreting cells. KEYWORDS: Cytoplasm;Phosphoprotein;Reference proteome;SH3 domain SUBCELLULAR LOCATION: Cytoplasm Note=Accumulates in cell surface projections. G3XCV0,"PROTEIN NAMES: Transcriptional regulator FleQ ORGANISM: Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) FUNCTION: AAA+ ATPase enhancer-binding protein that acts as a transcription regulator and plays a role in the modulation of mucin adhesion and flagellar gene expression. In addition to flagella genes, regulates also expression of biofilm-related genes. Functions as a transcriptional repressor in the absence of c-di-GMP and as an activator when c-di-GMP is present. KEYWORDS: 3D-structure;ATP-binding;DNA-binding;Nucleotide-binding;Reference proteome;Transcription;Transcription regulation " G3XD94,PROTEIN NAMES: UDP-N-acetyl-D-glucosamine 6-dehydrogenase (UDP-D-GlcNAc 6-dehydrogenase) (UDP-N-acetylglucosamine 6-dehydrogenase) PROTEIN FAMILY: UDP-glucose/GDP-mannose dehydrogenase family ORGANISM: Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) SIMILARITY: Belongs to the UDP-glucose/GDP-mannose dehydrogenase family. FUNCTION: Plays a role in the biosynthesis of B-band O antigen for serotype O5. Catalyzes the C-6 dehydrogenation of UDP-D-GlcNAc to UDP-N-acetylglucosaminuronic acid (UDP-D-GlcNAcA). PATHWAY: Bacterial outer membrane biogenesis; LPS O-antigen biosynthesis. KEYWORDS: Cell wall biogenesis/degradation;Lipopolysaccharide biosynthesis;Membrane;NAD;Oxidoreductase;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Single-pass membrane protein G4MY67,PROTEIN NAMES: Protein farnesyltransferase subunit beta (FTase-beta) (PFTase beta) (CAAX farnesyltransferase subunit beta) (Ras proteins prenyltransferase subunit beta) PROTEIN FAMILY: Protein prenyltransferase subunit beta family ORGANISM: Pyricularia oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast fungus) (Magnaporthe oryzae) SIMILARITY: Belongs to the protein prenyltransferase subunit beta family. FUNCTION: Catalyzes the transfer of a farnesyl moiety from farnesyl diphosphate to a cysteine at the fourth position from the C-terminus of several proteins having the C-terminal sequence Cys-aliphatic-aliphatic-X. The beta subunit is responsible for peptide-binding. KEYWORDS: Cytoplasm;Metal-binding;Prenyltransferase;Reference proteome;Repeat;Transferase;Zinc SUBCELLULAR LOCATION: Cytoplasm G4WJD4,"PROTEIN NAMES: GDP-4-keto-6-deoxy-D-mannose 3-dehydratase PROTEIN FAMILY: DegT/DnrJ/EryC1 family ORGANISM: Yersinia pseudotuberculosis SIMILARITY: Belongs to the DegT/DnrJ/EryC1 family. FUNCTION: Involved in the biosynthesis of L-colitose, a 3,6-dideoxyhexose present in the O-antigen region of lipopolysaccharides (LPS), where it serves as an antigenic determinant and is vital for bacterial defense and survival. Catalyzes the removal of the C3'-hydroxyl group from GDP-4-keto-6-deoxy-D-mannose via a combined transamination-deoxygenation reaction. The catalysis is initiated by a transamination step in which pyridoxal 5'-phosphate (PLP) is converted to pyridoxamine 5'-phosphate (PMP) in the presence of L-glutamate. This coenzyme then forms a Schiff base with GDP-4-keto-6-deoxy-D-mannose and the resulting adduct undergoes a PMP-mediated beta-dehydration reaction to give a sugar enamine intermediate, which after tautomerization and hydrolysis to release ammonia yields GDP-4-keto-3,6-dideoxy-D-mannose as a product. PATHWAY: Nucleotide-sugar metabolism; GDP-L-colitose biosynthesis. KEYWORDS: Cell membrane;Lyase;Membrane;Pyridoxal phosphate;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass membrane protein " G5EBK1,"PROTEIN NAMES: Na(+)/H(+) exchanger protein 7 (Na(+)/H(+) antiporter nhx-7) (PBoc defective protein pbo-4) PROTEIN FAMILY: Monovalent cation:proton antiporter 1 (CPA1) transporter family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family. FUNCTION: Na+/H+ exchanger which mediates the transient acidification of the coelomic space and plays a role in contraction of posterior body muscles during defecation. Probably by regulating the defecation motor program, required for fatty acid uptake by intestinal cells. KEYWORDS: Antiport;Calmodulin-binding;Cell membrane;Coiled coil;Glycoprotein;Ion transport;Membrane;Reference proteome;Sodium;Sodium transport;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Basolateral cell membrane ; Multi-pass membrane protein " G5EBR3,"PROTEIN NAMES: Glutamate-gated chloride channel alpha (Avermectin-sensitive glutamate-gated chloride channel GluCl alpha) (GluCl alpha) PROTEIN FAMILY: Ligand-gated ion channel family, Glutamate-gated chloride channel subfamily ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the ligand-gated ion channel (TC 1.A.9) family. Glutamate-gated chloride channel (TC 1.A.9.4) subfamily. FUNCTION: Glutamate-gated chloride channel subunit; channel properties depend on the subunit composition. Glutamate binding triggers a rapidly reversible current in heteromeric channels formed by glc-1 and glc-2, while the anti-helmintic drug ivermectin and other avermectins trigger a permanently open channel configuration. Channels containing only glc-1 are activated by ivermectin, but not by glutamate alone (in vitro). The heteromeric channel formed by glc-1 and glc-2 is also activated by ibotenate, and it is blocked by picrotoxin and flufenamic acid. Plays a role in the regulation of locomotor behavior. KEYWORDS: 3D-structure;Cell membrane;Chloride;Disulfide bond;Glycoprotein;Ion channel;Ion transport;Membrane;Postsynaptic cell membrane;Reference proteome;Signal;Synapse;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Postsynaptic cell membrane ; Multi-pass membrane protein Cell membrane ; Multi-pass membrane protein " G5EBU4,"PROTEIN NAMES: Zinc finger E-box-binding homeobox protein zag-1 (Zinc finger involved in axon guidance 1) (ZAG-1) ORGANISM: Caenorhabditis elegans FUNCTION: Transcription factor. Down-regulates expression of genes involved in either the synthesis or reuptake of serotonin, dopamine and GABA. Acts as a transcriptional repressor to regulate multiple, discrete, neuron-specific aspects of terminal differentiation, including cell migration, axonal development and gene expression. Promotes touch receptor neuron differentiation by repressing the expression of egl-44 and egl-46. As egl-44 and egl-46, probably acting as a heterodimer, repress expression of zag-1 in FLP neurons, together these proteins form a bistable, negative-feedback loop that regulates the choice between neuronal fates. Required for axon guidance. Involved in the proper development of the pharynx. Required for pharynx isthmus peristalsis, probably via a role in the differentiation of the M4 cholinergic motor neuron. Directly represses its own transcription by interacting with conserved E-box sequence motifs 5'-CACCTG-3' in its own promoter. May also act as a transcriptional activator of the homeodomain ceh-28. KEYWORDS: Developmental protein;DNA-binding;Homeobox;Metal-binding;Nucleus;Reference proteome;Repeat;Repressor;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus " G5EBV0,"PROTEIN NAMES: Hypoxia-inducible factor prolyl hydroxylase (HIF-PH) (Egg-laying defective protein 9) (Hypoxia-inducible factor-proline dioxygenase) ORGANISM: Caenorhabditis elegans FUNCTION: Cellular oxygen sensor which regulates the stability and the activity of hypoxia-inducible transcription factor, hif-1. In normoxic conditions, hydroxylates hif-1 targeting it for vhl-1-mediated proteasomal degradation. In addition, regulates hif-1 transcriptional activity in a vhl-1-independent manner and independently of its hydroxylase activity. By regulating hif-1 activity, controls several cellular responses. Mediates susceptibility to B.thuringiensis and V.cholerae pore-forming toxins and enteropathogenic E.coli. Mediates susceptibility to P.aeruginosa PAO1-mediated killing by regulating resistance to cyanide produced by P.aeruginosa. Mediates resistance to S.aureus-mediated killing. In addition, plays a role in heat acclimation, neuronal development, behavioral responses to reoxygenation and hydrogen sulfide, iron homeostasis and aging. In neurons, involved in mitochondrion fusion during reoxygenation. Involved in egg laying.; FUNCTION: [Isoform e]: Regulates the trafficking of the glutamate receptor glr-1, probably independently of hif-1, by regulating lin-10 subcellular localization in response to oxygen levels. May hydroxylate lin-10. KEYWORDS: Alternative splicing;Cell projection;Cytoplasm;Dioxygenase;Iron;Metal-binding;Nucleus;Oxidoreductase;Reference proteome;Vitamin C;Zinc;Zinc-finger SUBCELLULAR LOCATION: [Isoform a]: Cytoplasm Nucleus Cell projection, dendrite Note=In interneurons, localizes throughout the ventral cord dendrites.; SUBCELLULAR LOCATION: [Isoform c]: Cytoplasm Nucleus Cell projection, dendrite Note=In interneurons, localizes throughout the ventral cord dendrites.; SUBCELLULAR LOCATION: [Isoform e]: Cytoplasm Nucleus Cell projection, axon Note=In interneurons, localizes in puncta (which probably correspond to endosomes) along the ventral cord. Localization to puncta is regulated by oxygen levels. Co-localizes with lin-10 in puncta. " G5EBX9,"PROTEIN NAMES: Serine/threonine-protein phosphatase with EF-hands pef-1 (CePPEF) (Phosphatase with EF hands 1) PROTEIN FAMILY: PPP phosphatase family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the PPP phosphatase family. FUNCTION: Probably acts as a protein phosphatase. KEYWORDS: Alternative splicing;Calcium;Cell membrane;Cell projection;Hydrolase;Lipoprotein;Manganese;Membrane;Metal-binding;Myristate;Palmitate;Reference proteome;Repeat SUBCELLULAR LOCATION: Cell membrane ; Lipid-anchor Perikaryon Cell projection, dendrite Cell projection, axon Cell projection, cilium " G5EBY8,"PROTEIN NAMES: BMP-like protein unc-129 (Uncoordinated protein 129) PROTEIN FAMILY: TGF-beta family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the TGF-beta family. FUNCTION: Required for the migration of axonal growth-cones and distal tip cells (DTC) along the dorsal-ventral axis of the body wall. Acts cell nonautonomously and independently of the classical daf-4, sma-6 or daf-1 TGFbeta receptor signaling. During axon migration, facilitates long-range repulsive guidance of unc-6/netrin by enhancing unc-5-unc-40 signaling at the expense of unc-5 alone signaling, probably through direct interaction with receptor unc-5. Involved in cell-cell contact formation in sensory rays in the developing male tail, via a pathway involving plx-2 and mab-20/semaphorin-2A. KEYWORDS: Glycoprotein;Growth factor;Neurogenesis;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted, extracellular space " G5EC37,"PROTEIN NAMES: Protein sepa-1 (Suppressor of ectopic P granules in autophagy) ORGANISM: Caenorhabditis elegans FUNCTION: Adapter protein that connects P-granules in somatic cells with the autophagic machinery. Association with other adapters such as epg-2 and P-granule components such as pgl-3 is required for the accumulation and degradation of P-granules by autophagy in somatic cells. This ensures exclusive localization of the P-granules in germ cells. KEYWORDS: Coiled coil;Cytoplasm;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus Cytoplasm Cytoplasmic granule Note=Diffuse cytoplasmic localization, but also localized to cytoplasmic aggregates throughout development. Co-localizes with epg-2 and lgg-1 in cytoplasmic aggregates. " G5ECD9,"PROTEIN NAMES: G-protein coupled receptor aex-2 (Aboc, expulsion defective protein 2) PROTEIN FAMILY: G-protein coupled receptor 1 family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the G-protein coupled receptor 1 family. FUNCTION: G-protein coupled receptor for the nlp-40 neuropeptide. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. Plays a role in the defecation motor program, which is a coordinated series of three muscle contractions that occurs every 45 seconds. Specifically, acts in GABAergic neurons, such as AVL and DVB, to control the expulsion step of defecation. Required for fatty acid uptake and metabolism by intestinal cells and therefore regulates the levels of triglycerides in the intestine. KEYWORDS: Cell membrane;Cell projection;Disulfide bond;G-protein coupled receptor;Glycoprotein;Membrane;Receptor;Reference proteome;Transducer;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Cell projection, cilium " G5ECJ6,"PROTEIN NAMES: Tyrosine-protein kinase csk-1 (C-terminal src kinase) PROTEIN FAMILY: Protein kinase superfamily, Tyr protein kinase family, CSK subfamily ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. CSK subfamily. FUNCTION: Non-receptor tyrosine-protein kinase which plays a role in pharynx function by regulating pumping and the orientation of pharyngeal muscle fibers, independently of src-1 and src-2. May phosphorylate and thereby negatively regulate src-1 and src-2 activities. KEYWORDS: Alternative splicing;ATP-binding;Kinase;Magnesium;Manganese;Metal-binding;Nucleotide-binding;Reference proteome;SH2 domain;SH3 domain;Transferase;Tyrosine-protein kinase " G5ECP4,"PROTEIN NAMES: Pelle-like serine/threonine-protein kinase pik-1 PROTEIN FAMILY: Protein kinase superfamily, TKL Ser/Thr protein kinase family, Pelle subfamily ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily. FUNCTION: Through association with the adapter actl-1, may act downstream of the receptor complex composed of ilcr-1 and ilcr-2, which is a signaling complex that modulates neuronal activity and animal behavior in response to sensory neuron input. KEYWORDS: Alternative splicing;ATP-binding;Kinase;Nucleotide-binding;Reference proteome;Serine/threonine-protein kinase;Transferase " G5ECR9,"PROTEIN NAMES: Nuclear hormone receptor unc-55 (Uncoordinated protein 55) PROTEIN FAMILY: Nuclear hormone receptor family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the nuclear hormone receptor family. FUNCTION: Transcription factor. Involved in motor neuron fate determination and maintenance, acting as a transcriptional repressor to counteract gene activation by transcription factors unc-3 or irx-1 in subsets of motor neurons. Probably acts by binding to specific promoter elements. Required for ventral D (VD) motor neurons to adopt their normal synaptic pattern. Suppresses expression of flp-13 in VD motor neurons to ensure formation of the correct synaptic pattern. Maintains low cAMP levels in VD motor neurons by enhancing expression of pde-4 which hydrolyzes cAMP and repressing expression of acy-1 which catalyzes cAMP formation. This prevents respecification of synapses by VD neurons. During copulation, required in males for correct movement of the spicules, a pair of prong-like structures which are inserted into the vulva of the hermaphrodite and anchor the male to the hermaphrodite. Required for spicule prodding which allows detection of the vulva location and for spicule insertion into the vulva.; FUNCTION: [Isoform a]: Required for normal locomotion. KEYWORDS: Activator;Alternative splicing;DNA-binding;Metal-binding;Nucleus;Receptor;Reference proteome;Repressor;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus " G5ECU7,"PROTEIN NAMES: Transcription factor jun-1 (Transcription factor AP-1 subunit jun-1) PROTEIN FAMILY: BZIP family, Jun subfamily ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the bZIP family. Jun subfamily. FUNCTION: Transcription factor that recognizes and binds to the AP-1 non-canonical enhancer heptamer motif 5'-TTAGTCA-3'. Required for ovulation. Controls plc-1 expression in the spermatheca to regulate spermathecal valve dilation. KEYWORDS: Alternative splicing;DNA-binding;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " G5ED68,"PROTEIN NAMES: Myotubularin-related protein 6 (Phosphatidylinositol-3-phosphate phosphatase) PROTEIN FAMILY: Protein-tyrosine phosphatase family, Non-receptor class myotubularin subfamily ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the protein-tyrosine phosphatase family. Non-receptor class myotubularin subfamily. FUNCTION: May dephosphorylate phosphatidylinositol-3-phosphate (PI3P). In association with mtm-9, plays a role in endosome trafficking probably by regulating phosphatidylinositol-3-phosphate levels. Regulates fluid phase endocytosis in coelomocytes. Controls the endosomal localization of sorting nexin snx-3 and the levels of sorting receptor mig-14. By regulating the retrograde transport of mig-14, may be involved in the secretion of Wnt ligands such as egl-20. Regulates posterior migration of QL neuroblast descendants and the anterior migration of QR neuroblast descendants and HSN neurons during larval development. Involved in the formation of correct synapse number in DA9 motor neurons probably in part by regulating the secretion of Wnt ligand egl-20. KEYWORDS: Alternative splicing;Cell membrane;Cytoplasm;Endocytosis;Hydrolase;Lipid metabolism;Membrane;Metal-binding;Reference proteome;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cytoplasm Membrane ; Peripheral membrane protein Apical cell membrane ; Peripheral membrane protein " G5EDB2,"PROTEIN NAMES: Probable dual specificity protein kinase madd-3 (Muscle arm development defective protein 3) PROTEIN FAMILY: Protein kinase superfamily, CMGC Ser/Thr protein kinase family, Lammer subfamily ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. Lammer subfamily. FUNCTION: [Isoform a]: Probable dual specificity kinase acting on both serine/threonine and tyrosine-containing substrates. Negatively regulates p38 MAPK signaling to allow for the plasma membrane of body wall muscle cells to form projections, also called muscle arms, that extend and connect the body wall muscles to target motor neurons. Negative regulation of p38 MAPK signaling may in turn modulate the trafficking of the muscle specific receptor eva-1 to the lysosome, to ensure proper display of the eva-1 receptor on the plasma membrane of muscle cells and allow for muscle arm extension towards guidance cues. KEYWORDS: Alternative splicing;ATP-binding;Cytoplasm;Kinase;Nucleotide-binding;Nucleus;Reference proteome;Serine/threonine-protein kinase;Transferase;Tyrosine-protein kinase SUBCELLULAR LOCATION: [Isoform a]: Cytoplasm Nucleus Note=Enriched in the cytoplasm. " G5EDF0,"PROTEIN NAMES: Grainyhead-like protein 1 homolog PROTEIN FAMILY: Grh/CP2 family, Grainyhead subfamily ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the grh/CP2 family. Grainyhead subfamily. FUNCTION: Probable transcription factor. Binds a motif with the core sequence 5'-C[ACT][TG]G-3' in regulatory elements of target genes. Many putative target genes show oscillating expression levels, perhaps as a result of rhythmic variation in accumulation of grh-1. Plays a role in proper cuticle formation and/or barrier function and is required repetitively during development, for successful completion of each molt. Involved in modulating lifespan. Plays a role in defense response to bacteria. May act upstream of the p38 MAP kinase / pmk-1 pathway. May act downstream of the insulin/IGF-1 receptor signaling (IIS) pathway. KEYWORDS: DNA-binding;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " G5EDM4,"PROTEIN NAMES: Na(+)/H(+) exchange regulatory cofactor-like protein nrfl-1 (NHERF-1) (Regulatory cofactor of Na(+)/H(+) exchanger) (Sodium-hydrogen exchanger regulatory factor 1) ORGANISM: Caenorhabditis elegans FUNCTION: Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression (By similarity). Anchors the amino acid transporter protein aat-6 to the apical cell membrane of intestinal cells, particularly in older animals, in order to maintain amino acid homeostasis. May play a role in promoting fertility. KEYWORDS: Alternative splicing;Cell membrane;Cell projection;Membrane;Reference proteome;Repeat SUBCELLULAR LOCATION: Cell projection, microvillus membrane Apical cell membrane Note=Co-localizes with aat-6 at the apical cell membrane of intestinal cells. " G5EDN0,"PROTEIN NAMES: Acetylcholine-gated chloride channel subunit acc-3 PROTEIN FAMILY: Ligand-gated ion channel family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the ligand-gated ion channel (TC 1.A.9) family. FUNCTION: Probable acetylcholine-gated chloride channel regulatory subunit. Does not have ion channel activity alone as a homopentamer, but forms a functional heteropentameric ion channel with acc-1. KEYWORDS: Cell membrane;Chloride;Chloride channel;Disulfide bond;Glycoprotein;Ion channel;Ion transport;Ligand-gated ion channel;Membrane;Reference proteome;Signal;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein " G5EDW7,PROTEIN NAMES: Protein lin-8 (Abnormal cell lineage protein 8) PROTEIN FAMILY: Lin-8 family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the lin-8 family. FUNCTION: Acts as a synthetic multivulva class A (synMuvA) protein and redundantly inhibits lin-3/EGF expression to prevent inappropriate vulva induction. KEYWORDS: Developmental protein;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus G5EE01,"PROTEIN NAMES: Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase daf-18 (Abnormal dauer formation protein 18) PROTEIN FAMILY: PTEN phosphatase protein family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the PTEN phosphatase protein family. FUNCTION: Acts as a dual-specificity protein phosphatase, dephosphorylating tyrosine-, serine- and threonine-phosphorylated proteins (By similarity). Also acts as a lipid phosphatase, removing the phosphate in the D3 position of the inositol ring from phosphatidylinositol 3,4,5-trisphosphate. By dephosphorylating PtdIns(3,4,5)P3 antagonizes PtdIns(3,4,5)P3 production by age-1/PI3K and thus, negatively regulates daf-2-mediated processes including dauer formation, longevity, fat metabolism, chemotaxis towards salt, thermotolerance and axon guidance. Similarly, promotes apoptosis during embryonic development by suppressing the recruitment of the prosurvival kinases akt-1/2 to the plasma membrane. In addition, regulates Z2/Z3 germline precursor cell cycle by maintaining them arrested at the G2 stage and by controlling their growth during L1 diapause. After sperm depletion in larvae and adult hermaphrodites, promotes germline stem cell quiescence and oocyte accumulation. By dephosphorylating ephrin-like receptor vab-1 on tyrosine residues, negatively regulates oocyte maturation downstream of vab-1 and upstream of mpk-1, independently of daf-2. Plays a role in postembryonic muscle arm extensions. Required for neurite outgrowth during AIY interneuron embryonic development. Mainly independently of daf-2, negatively regulates vulva induction probably by inhibiting mpk-1 phosphorylation. Both lipid and protein phosphatase activities are required for the regulation of vulva induction. Plays a role in gonad and germline development following the L1 diapause. KEYWORDS: Cell membrane;Cell projection;Cytoplasm;Hydrolase;Lipid metabolism;Lipid-binding;Membrane;Nucleus;Phosphoprotein;Protein phosphatase;Reference proteome SUBCELLULAR LOCATION: Perikaryon Cell membrane ; Peripheral membrane protein Cell projection, axon Cell projection, dendrite Cytoplasm Nucleus Note=During vulva development, localizes to the cytoplasm and the nucleus of vulva precursor cells and of vulva cells at the Pn.p stage of L2 larvae. From the Pn.px to Pn.pxx stage of L3/L4 larvae, localization increases at the plasma membrane and becomes maximal at the Pn.pxxx stage of L4 larvae. " G5EED4,"PROTEIN NAMES: Protein nipi-3 (No induction of peptide after drechmeria infection protein 3) (Tribbles homolog nipi-3) PROTEIN FAMILY: Protein kinase superfamily, CAMK Ser/Thr protein kinase family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. FUNCTION: Adapter protein that regulates different signaling pathways (By similarity). Required for larval development and viability. Involved in negatively modulating pmk-1 p38/MAPK signaling. Involved in innate immunity, acting either in a manner dependent upon, or independent of, the pmk-1 or pmk-3 p38/MAPK pathways. Has a protective role in response to infection by the Gram-negative bacterium P.aeruginosa, acting by negatively modulating expression of cebp-1, and regulating the pmk-1 p38/MAPK pathway, leading to activation of transcription factor skn-1. Required to prevent P.aeruginosa toxin ToxA-mediated lethality, probably acting via modulating the effects of translational inhibition caused by the toxin. By regulating the up-regulation in the epidermis of antimicrobial peptides nlp-29 and nlp-31, plays a role in resistance to fungal infection. KEYWORDS: ATP-binding;Nucleotide-binding;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus " G5EEG1,"PROTEIN NAMES: Nuclear transcription factor Y subunit nfya-1 (CAAT box DNA-binding protein subunit nfya-1) PROTEIN FAMILY: NFYA/HAP2 subunit family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the NFYA/HAP2 subunit family. FUNCTION: Component of the sequence-specific heterotrimeric transcription factor (nfya-1-NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes to regulate their expression and control cellular identity in particular tissue types. In association with the components in the nfya-1-NF-Y complex, represses the expression of the T-box transcription factor tbx-2 throughout larval development, which most likely restricts its expression to certain tissues. May act to repress txb-2 expression in conjunction with tbx-2 itself, which has an autoregulatory role (Probable). With the components in this complex, negatively regulates the expression of the homeobox protein egl-5 to spatially restrict its expression in tissues such as the head. May regulate egl-5 expression in association with the mes-2-mes-3-mes-6 complex. KEYWORDS: DNA-binding;Nucleus;Reference proteome;Repressor;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " G5EEI4,"PROTEIN NAMES: Aspartic protease 1 PROTEIN FAMILY: Peptidase A1 family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the peptidase A1 family. FUNCTION: Aspartic protease, which is part of the necrosis cell death pathway. Promotes B.thuringiensis Cry6Aa stability by preventing its proteolysis by host gut proteases. Required for Cry6Aa-induced necrotic death of intestinal cells. Cry6Aa uptake into the host intestinal cells triggers an increase in intracellular Ca(2+) levels leading to lysosome rupture and to the subsequent release of asp-1 which leads to necrosis. KEYWORDS: Aspartyl protease;Cytoplasm;Disulfide bond;Glycoprotein;Hydrolase;Lysosome;Necrosis;Protease;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Cytoplasm Lysosome Secreted " G5EEM0,"PROTEIN NAMES: Nuclear hormone receptor 114 PROTEIN FAMILY: Nuclear hormone receptor family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the nuclear hormone receptor family. FUNCTION: Probable transcription factor which may have a role in detoxifying dietary metabolites arising from bacterial tryptophan metabolism. Required for fertility and involved in proper postembryonic germline development, especially germline stem cell (GSC) proliferation. Required for activation of the methionine/S-adenosylmethionine (Met/SAM) cycle in response to low levels of SAM. KEYWORDS: Alternative splicing;DNA-binding;Metal-binding;Nucleus;Receptor;Reference proteome;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus " G5EF60,"PROTEIN NAMES: Stromal interaction molecule 1 ORGANISM: Caenorhabditis elegans FUNCTION: Plays a role in mediating store-operated Ca(2+) entry (SOCE), a Ca(2+) influx following depletion of intracellular Ca(2+) stores. Acts as a Ca(2+) sensor which upon Ca(2+) depletion, activates the Ca(2+) release-activated Ca(2+) (CRAC) channel subunit, orai-1. Essential for Ca (2+) and IP3-dependent contractile activity of gonad sheath cells and spermatheca. Essential for fertility. Does not play a role in posterior body wall muscle contraction (pBoc) rhythmicity, intestinal cell oscillatory Ca(2+) signaling or intestinal ER Ca(2+) hemostasis. KEYWORDS: 3D-structure;Alternative splicing;Calcium;Calcium channel;Calcium transport;Coiled coil;Ion channel;Ion transport;Membrane;Metal-binding;Reference proteome;Signal;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Membrane ; Single-pass type I membrane protein Note=Localizes to intracellular puncta in the anterior intestine and reticular structure in the posterior intestine. " G5EFD4,"PROTEIN NAMES: Heavy metal tolerance factor 1 PROTEIN FAMILY: ABC transporter superfamily, ABCB family, Heavy Metal importer subfamily ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the ABC transporter superfamily. ABCB family. Heavy Metal importer (TC 3.A.1.210) subfamily. FUNCTION: May play a pivotal role in the detoxification of heavy metals such as cadmium but do not depend exclusively on phytochelatins (PC) synthesis. KEYWORDS: ATP-binding;Endosome;Membrane;Nucleotide-binding;Reference proteome;Transmembrane;Transmembrane helix;Transport;Vacuole SUBCELLULAR LOCATION: Vacuole membrane ; Multi-pass membrane protein Early endosome Late endosome Recycling endosome " G5EFE7,"PROTEIN NAMES: Alpha-(1,6)-fucosyltransferase (Alpha1-6FucT) (Fucosyltransferase 8) (GDP-L-Fuc:N-acetyl-beta-D-glucosaminide alpha1,6-fucosyltransferase) (GDP-fucose--glycoprotein fucosyltransferase) (Glycoprotein 6-alpha-L-fucosyltransferase) PROTEIN FAMILY: Glycosyltransferase 23 family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the glycosyltransferase 23 family. FUNCTION: Catalyzes the addition of fucose in alpha 1-6 linkage to the first GlcNAc residue, next to the peptide chains in N-glycans. The addition is prevented if the GlcNAc residue is already fucosylated. Involved in susceptibility to the nematotoxic C.cinerea galectin Cgl2, likely by contributing to the synthesis of core alpha-1,6-fucosylated N-glycans to which Cgl2 binds. PATHWAY: Protein modification; protein glycosylation. KEYWORDS: Disulfide bond;Glycoprotein;Glycosyltransferase;Golgi apparatus;Magnesium;Manganese;Membrane;Metal-binding;Reference proteome;SH3 domain;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus, Golgi stack membrane ; Single-pass type II membrane protein Note=Membrane-bound form in trans cisternae of Golgi. " G5EFF1,"PROTEIN NAMES: RNA-binding protein asd-2 (Alternative splicing defective protein 2) ORGANISM: Caenorhabditis elegans FUNCTION: RNA-binding protein that binds to the 5'-NACUAAY-N(1,20)-UAAY-3' consensus sequence in pre-mRNA introns to promote alternative splicing. Required for mutually exclusive alternative splicing where it modulates the switch between mutually exclusive exons during pre-mRNA maturation. Involved in muscle-specific gene expression regulating the alternative splicing of genes such as let-2 and unc-60 to ensure that their respective isoforms are expressed in muscle. Promotes the removal of intron 10 from let-2 pre-mRNA to allow for the exclusive expression of the muscle-specific let-2 isoform (as opposed to the non-muscle-specific isoform expressed in embryos) in body wall muscles during late larval and adult stages of development. Binds cooperatively with RNA-binding protein sup-12 to intron 1A of the unc-60 pre-mRNA to promote alternative splicing and expression of the muscle specific isoform of unc-60. KEYWORDS: Alternative splicing;mRNA processing;mRNA splicing;Nucleus;Reference proteome;RNA-binding SUBCELLULAR LOCATION: Nucleus " G5EFL0,"PROTEIN NAMES: Poly(A) RNA polymerase gld-4 (Defective in germ line development protein 4) (Germline development defective-4) ORGANISM: Caenorhabditis elegans FUNCTION: Cytoplasmic poly(A) RNA polymerase that adds successive AMP monomers to the 3'-end of specific RNAs, forming a poly(A) tail. The enzymatic activity is enhanced by its interaction with gls-1. Required, together with gld-2, for early meiotic progression in male and female germ cells and for gld-1 protein accumulation in the hermaphrodite germline. In the germline, forms a complex with gls-1 which directly binds to gld-1 mRNA and prevents its degradation. KEYWORDS: ATP-binding;Cytoplasm;Developmental protein;Magnesium;Manganese;Meiosis;Metal-binding;mRNA processing;Nucleotide-binding;Reference proteome;Transferase SUBCELLULAR LOCATION: Cytoplasm Cytoplasmic granule Cytoplasm, perinuclear region Note=Localizes to P granules. This association is less apparent during pachytene, becomes obvious in maturing oocytes and is most prominently visible in developing embryos. MISCELLANEOUS: The gld-4 locus produces a single transcript that is SL-1 trans-spliced." G5EFP5,"PROTEIN NAMES: Alpha-(1,3)-fucosyltransferase fut-3 (Fucosyltransferase fut-3) PROTEIN FAMILY: Glycosyltransferase 10 family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the glycosyltransferase 10 family. FUNCTION: Catalyzes the addition of fucose in alpha 1-3 linkage. Unlike fut-1, does not add fucose to Man-alpha-1->3-(Man-alpha-1->6)-Man-beta-1->4-GlcNAc-beta-1->4-GlcNAc-beta-1-Asn (M3), Man-alpha-1->3-(Man-alpha-1->6)-Man-beta-1->4-GlcNAc-beta-1->4-(Fuc-alpha-1->6)-GlcNAc-beta-1-Asn (M3F6) or GlcNAc-beta-1->2-Man-alpha-1->3-(GlcNAc-beta-1->2-Man-alpha-1->6)-Man-beta-1-4-GlcNAc-beta-1->4-(Fuc-alpha-1->6)-GlcNAc-beta-1-Asn (GnM3F6) acceptors. PATHWAY: Protein modification; protein glycosylation. KEYWORDS: Glycoprotein;Glycosyltransferase;Golgi apparatus;Membrane;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus, Golgi stack membrane ; Single-pass type II membrane protein " G5EFT4,"PROTEIN NAMES: Aminopeptidase ltah-1.1 (Aminopeptidase-1) (AP-1) (Arginine aminopeptidase 1) (Leukotriene A4 hydrolase homolog ltah-1.1) PROTEIN FAMILY: Peptidase M1 family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the peptidase M1 family. FUNCTION: Aminopeptidase which preferentially removes N-terminal Arg and Lys residues from peptides and proteins. KEYWORDS: Alternative splicing;Aminopeptidase;Cytoplasm;Hydrolase;Metal-binding;Metalloprotease;Protease;Reference proteome;Zinc SUBCELLULAR LOCATION: Cytoplasm MISCELLANEOUS: Despite its similarity to leukotriene hydrolases, AP-1 does not have leukotriene hydrolase activity. One of the mammalian leukotriene binding sites, 'Tyr-378', is replaced by a Phe residue in AP-1." G5EFU0,"PROTEIN NAMES: Serine/threonine-protein kinase pak-2 (p21-activated kinase 2) PROTEIN FAMILY: Protein kinase superfamily, STE Ser/Thr protein kinase family, STE20 subfamily ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. FUNCTION: Serine/threonine-protein kinase which plays a redundant role with pak-1 in embryogenesis but, in contrast to pak-1, is not involved in commissural axon guidance of ventral cord motoneurons or in distal tip cell (DTC) migration. KEYWORDS: Alternative splicing;ATP-binding;Kinase;Magnesium;Manganese;Metal-binding;Nucleotide-binding;Reference proteome;Serine/threonine-protein kinase;Transferase " G5EFZ3,"PROTEIN NAMES: Set1/Ash2 histone methyltransferase complex subunit ash-2 ORGANISM: Caenorhabditis elegans FUNCTION: Component of the set-2/ash-2 histone methyltransferase (HMT) complex (Probable). Required for the di- and trimethylation at 'Lys-4' of histone H3, a mark associated with epigenetic transcriptional activation. Implicated in the epigenetic inheritance of lifespan over several generations. Functions as a transcriptional regulator. Acts in the germline to limit the longevity of the soma, probably by regulating a lipid metabolism pathway that signals from the germline to the intestine, thereby preventing accumulation of mono-unsaturated fatty acids. KEYWORDS: Chromatin regulator;DNA-binding;Metal-binding;Nucleus;Reference proteome;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus " G5EG11,"PROTEIN NAMES: Delta(5) fatty acid desaturase fat-4 (FAT-4) (FAT-4 Delta(5) desaturase) (Fatty acid desaturase 4) PROTEIN FAMILY: Fatty acid desaturase type 1 family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the fatty acid desaturase type 1 family. FUNCTION: Can function as a Delta(5) fatty acid desaturase and behaves as a (8-3) desaturase. Introduces a double bond in the fatty acid chain 5 carbons away from carboxy terminal to biosynthesize polyunsaturated fatty acids (PUFAs) endogenously (PUFAs are essential for membrane structure and many cellular and physiological processes). Acts on a variety of substrates such as dihomo-gamma-linoleoyl-CoA ((8Z,11Z,14Z)-eicosatrienoyl-CoA, 20:3n-6) to generate arachidonoyl-CoA ((5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA, 20:4n-6). Also acts on a number of other substrates, including fatty acids that do not contain a double bond at the 8 position like (11Z,14Z,17Z)-eicosatrienoyl-CoA (20:3n-3) to produce (5Z,11Z,14Z,17Z)-eicosatetraenoyl-CoA (20:4n-3). Unlike plants, Caenorhabditis elegans desaturases seem to use fatty acyl-CoAs as substrates (By similarity). PATHWAY: Lipid metabolism; polyunsaturated fatty acid biosynthesis. KEYWORDS: Alternative splicing;Fatty acid biosynthesis;Fatty acid metabolism;Lipid biosynthesis;Lipid metabolism;Membrane;Oxidoreductase;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Multi-pass membrane protein MISCELLANEOUS: HPO-19 and T05H4.4 are cytochrome b5 reductases required for PUFA desaturation in Caenorhabditis elegans. HPO-19 knockdown or mutation alters FAT-4 desaturase activity." G5EG14,PROTEIN NAMES: Splicing factor Cactin PROTEIN FAMILY: CACTIN family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the CACTIN family. FUNCTION: Plays a role in pre-mRNA splicing by facilitating excision of a subset of introns (By similarity). Plays a role during early embryonic development. Required for the distal tip cell migration at the end of larval development and for gonad morphogenesis. KEYWORDS: Alternative splicing;Coiled coil;Cytoplasm;Developmental protein;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus Cytoplasm G5EGQ3,"PROTEIN NAMES: Serine/threonine-protein kinase max-2 (Motor axon guidance protein 2) (p21-activated kinase) PROTEIN FAMILY: Protein kinase superfamily, STE Ser/Thr protein kinase family, STE20 subfamily ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. FUNCTION: Serine/threonine-protein kinase, which phosphorylates mlk-1. Involved in the stress response to heavy metals by activating the mlk-1/mek-1/kgb-1 pathway. In ventral cord commissural motoneurons, required for dorsal axon guidance downstream of unc-6/netrin repulsion receptor unc-5 and probably of Rho GTPases ced-10 and mig-2. Plays a redundant role with mig-10 in orientating axonal growth of HSN neurons. Plays a redundant role with pak-1 in P neuroblast migration and in distal tip cell (DTC)-mediated guidance of gonad elongation probably downstream of Rho GTPases. In association with pak-2, plays a role in embryogenesis. In association with pak-1, may be involved in spermatogenesis. KEYWORDS: Alternative splicing;ATP-binding;Cell projection;Cytoplasm;Differentiation;Gonadal differentiation;Kinase;Magnesium;Metal-binding;Neurogenesis;Nucleotide-binding;Reference proteome;Serine/threonine-protein kinase;Stress response;Transferase SUBCELLULAR LOCATION: Perikaryon Cell projection, dendrite Cytoplasm " G5EGT9,"PROTEIN NAMES: Inactive tyrosine-protein kinase RYK (Abnormal cell lineage protein 18) PROTEIN FAMILY: Protein kinase superfamily, Tyr protein kinase family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. FUNCTION: Has no detectable kinase activity in vitro and is unlikely to function as a tyrosine kinase in vivo (By similarity). Receptor which may act as a receptor for Wnt ligand mom-2. Plays a role in controlling P7.p vulva precursor cell lineage orientation during vulva development. Regulates pop-1 asymmetric distribution in P7.p and its daughter cells. Plays a role in the migration of ALM neurons during embryogenesis. KEYWORDS: ATP-binding;Cell membrane;Disulfide bond;Glycoprotein;Membrane;Neurogenesis;Nucleotide-binding;Receptor;Reference proteome;Signal;Transmembrane;Transmembrane helix;Wnt signaling pathway SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein Basolateral cell membrane ; Single-pass type I membrane protein Note=Localizes to the basolateral cell membrane of vulval precursor cells. " G5EGU2,"PROTEIN NAMES: Tyrosine-protein phosphatase non-receptor type eak-6 (Enhancer of akt-1 null 6) PROTEIN FAMILY: Protein-tyrosine phosphatase family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the protein-tyrosine phosphatase family. FUNCTION: Putative phosphatase which, together with eak-4 and sdf-9, negatively regulates dauer larva formation downstream of insulin-like receptor daf-2 and in parallel of age-1, pdk-1 and akt-1. KEYWORDS: Alternative splicing;Cell membrane;Cytoplasm;Hydrolase;Membrane;Protein phosphatase;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Cell membrane ; Peripheral membrane protein " G5EGU9,"PROTEIN NAMES: Suppressor of zyg-1 protein 20 ORGANISM: Caenorhabditis elegans FUNCTION: RNA binding protein that is required for normal cell division and cytokinesis during embryonic development. Functions with RNA-binding protein atx-2 to ensure embryonic cell division, and to this end, plays a role in the regulation of centrosome assembly, position and size, and in astral microtubule outgrowth and nucleation. Furthermore, negatively regulates the levels of the protein kinase zyg-1 at the centrosome. Also involved in ensuring centrosome attachment to the nuclear envelope. KEYWORDS: Alternative splicing;Cell cycle;Cell division;Chromosome;Cytoplasm;Cytoskeleton;Nucleus;Phosphoprotein;Reference proteome;RNA-binding SUBCELLULAR LOCATION: Cytoplasm Note=Co-localizes with atx-2 in the cytoplasm.; SUBCELLULAR LOCATION: [Isoform f]: Cytoplasm Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole Nucleus, nucleolus Chromosome Note=Co-localizes with sas-4 at centrioles. Localizes to nucleoli during interphase and to the surface of chromosomes during meiosis and mitosis. Localizes in puncta adjacent to the male pronucleus at the centrosome during meiosis. Localization at the centrosome peaks at prometaphase and metaphase, but then decreases and expression is at its most minimal during interphase. " G7KDA1,"PROTEIN NAMES: Low affinity inorganic phosphate transporter 8 (MtPT8) (MtPht1;8) (Arbuscular mycorrhiza-induced phosphate transporter PT8) (AM-induced phosphate transporter PT8) (H(+)/Pi cotransporter PT8) PROTEIN FAMILY: Major facilitator superfamily, Phosphate:H(+) symporter family ORGANISM: Medicago truncatula (Barrel medic) (Medicago tribuloides) SIMILARITY: Belongs to the major facilitator superfamily. Phosphate:H(+) symporter (TC 2.A.1.9) family. FUNCTION: Low-affinity transporter for external inorganic phosphate (Pi) that may be involved in the acquisition of phosphate released by arbuscular mycorrhizal (AM) fungi (e.g. Glomus versiforme and G.intraradices) during AM symbiosis; not required for mycorrhizal arbuscule development. KEYWORDS: Cell membrane;Glycoprotein;Hydrolase;Membrane;Phosphate transport;Reference proteome;Symport;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Note=Present on the periarbuscular membrane in cells containing arbuscules during arbuscular mycorrhizal (AM) symbiosis with AM fungi. MISCELLANEOUS: Although related to the sugar transporter family, it does not transport sugars." G8JZT0,PROTEIN NAMES: Outer membrane protein SusE (Starch-utilization system protein E) PROTEIN FAMILY: SusE family ORGANISM: Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50) SIMILARITY: Belongs to the SusE family. FUNCTION: Starch-binding protein present at the surface of the cell. Mediates starch-binding before starch transport in the periplasm for degradation. SusE and SusF do not constitute the major starch-binding proteins in starch degradation pathway. Has higher affinity for starch compared to SusF. PATHWAY: Glycan degradation; starch degradation. KEYWORDS: 3D-structure;Carbohydrate metabolism;Cell outer membrane;Lipoprotein;Membrane;Palmitate;Reference proteome;Signal SUBCELLULAR LOCATION: Cell outer membrane ; Lipid-anchor H2KYH4,"PROTEIN NAMES: Zinc finger protein ham-2 (HSN abnormal migration protein 2) ORGANISM: Caenorhabditis elegans FUNCTION: Probable transcription factor that acts downstream of egl-15, to promote migration of the HSN motor neurons from the tail to the gonad primordium during HSN cell differentiation. KEYWORDS: Alternative splicing;Metal-binding;Nucleus;Reference proteome;Repeat;Transcription;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus " H2KYS3,"PROTEIN NAMES: 3-ketosteroid oxygenase (Cytochrome P450 daf-9) (DAF-9) PROTEIN FAMILY: Cytochrome P450 family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the cytochrome P450 family. FUNCTION: Converts the 3-keto steroids 4-cholesten-3-one and lathosterone into the carboxylic metabolites 3-keto-4-cholestenate (Delta(4)-dafachronic acid, Delta(4)-DA) and 3-keto-7,(5a)-cholestenate (Delta(7)-dafachronic acid, Delta(7)-DA) respectively, by catalyzing successive oxidations at C-26. Dafachronic acids bind directly to the nuclear hormone receptor (NHR) DAF-12, suppressing dauer formation and inducing reproductive growth. In a non-cell autonomous manner, negatively regulates body wall muscle arm extensions to motor neurons probably by preventing daf-12 isoform b activation. May be involved in thermotolerance. PATHWAY: Steroid hormone biosynthesis; dafachronic acid biosynthesis. KEYWORDS: Alternative splicing;Cholesterol metabolism;Heme;Iron;Lipid metabolism;Membrane;Metal-binding;Monooxygenase;Oxidoreductase;Reference proteome;Steroid metabolism;Sterol metabolism;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Multi-pass membrane protein Note=Localizes to dendrite-like structure in XXXL/R cells. " H2KYU6,"PROTEIN NAMES: Nuclear protein localization protein 4 homolog 1 PROTEIN FAMILY: NPL4 family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the NPL4 family. FUNCTION: In association with ufd-1 and ATPase cdc-48.1 and/or cdc-48.2, involved in the cytoplasmic elimination of misfolded proteins exported from the ER. This pathway, known as ERAD, prevents the activation of the unfolded protein response (UPR) caused by the accumulation of misfolded proteins in the ER. During S phase and in association with ufd-1, cdc-48.1 and/or cdc-48.2 and ubxn-3, ensures the degradation of DNA licensing factor cdt-1 after the initiation of DNA replication and thus the disassembly of the DNA replication CGM helicase complex by promoting the dissociation from chromatin of several of its components including cdc-45 and sld-5. Regulates ubxn-3 nuclear localization during S phase. KEYWORDS: Alternative splicing;Chaperone;Cytoplasm;Metal-binding;Nucleus;Reference proteome;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cytoplasm Nucleus Note=Localizes to the cytoplasm during mitosis. Nuclear localization upon nuclear membrane re-assembly is cdc-48-dependent. " H2KZB2,"PROTEIN NAMES: Ankyrin repeat and LEM domain-containing protein 2 homolog (LEM domain-containing protein 4-like) PROTEIN FAMILY: ANKLE2 family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the ANKLE2 family. FUNCTION: Involved in mitotic nuclear envelope reassembly by promoting dephosphorylation of baf-1 during mitotic exit. Coordinates the control of baf-1 dephosphorylation by inhibiting VRK1 kinase and promoting dephosphorylation of baf-1 by protein phosphatase 2A (PP2A), thereby facilitating nuclear envelope assembly. It is unclear whether it acts as a real PP2A regulatory subunit or whether it is involved in recruitment of the PP2A complex. KEYWORDS: Alternative splicing;ANK repeat;Cell cycle;Cell division;Membrane;Mitosis;Nucleus;Reference proteome;Repeat;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Nucleus membrane ; Single-pass membrane protein " H2KZM9,"PROTEIN NAMES: Tubulin polyglutamylase ttll-11 (Tubulin--tyrosine ligase-like protein 11) PROTEIN FAMILY: Tubulin--tyrosine ligase family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the tubulin--tyrosine ligase family. FUNCTION: Polyglutamylase which preferentially modifies tubulin. Involved in the side-chain initiation step of the polyglutamylation reaction. By controlling tubulin glutamylation, regulates ciliary specialization and motor-based transport. Promotes the formation of A and B tubule singlets by splaying microtubule doublets in cilia. Together with ttll-4 and 5, required for male mating.; FUNCTION: [Isoform a]: Specifically promotes tubulin glutamylation in a subset of ciliated neurons including amphid, phasmid, CEP and RnA neurons.; FUNCTION: [Isoform b]: Specifically promotes tubulin glutamylation in male ciliated CEM, HOB and RnB neurons that release bioactive extracellular vesicles. Regulates the localization of TRP channel pdk-2 in male CEM, HOB and RnB neurons. Regulates the environmental release of bioactive extracellular vesicles in cilia. KEYWORDS: Alternative splicing;ATP-binding;Cell projection;Ligase;Nucleotide-binding;Reference proteome SUBCELLULAR LOCATION: Cell projection, axon Perikaryon Cell projection, dendrite Cell projection, cilium Extracellular vesicle " H2L008,"PROTEIN NAMES: Zinc finger protein ztf-16 PROTEIN FAMILY: Ikaros C2H2-type zinc-finger protein family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the Ikaros C2H2-type zinc-finger protein family. FUNCTION: Positively regulates the expression of ver-1 in the amphid sheath glia of amphid sensory neurons. Together with ehn-3, plays a role in somatic gonad development and is required for proper gonadal primordium assembly and somatic gonad precursor cell morphology. KEYWORDS: Alternative splicing;Metal-binding;Nucleus;Reference proteome;Repeat;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus " H2L056,"PROTEIN NAMES: UBX domain-containing protein 3 ORGANISM: Caenorhabditis elegans FUNCTION: Ubiquitin-binding protein which acts as an adapter for ATPase cdc-48.1 and/or cdc-48.2, conferring substrate specificity. Together with ubxn-1 and ubxn-2, plays a role in hermaphrodite spermatogenesis probably by promoting the degradation of sex determination terminal factor tra-1. During mitosis, ensures the degradation of DNA licensing factor cdt-1 and the disassembly of the DNA replication CMG helicase complex by promoting the dissociation from chromatin of several of its components including cdc-45 and sld-5. KEYWORDS: Alternative splicing;Chromosome;Coiled coil;Cytoplasm;Differentiation;Nucleus;Reference proteome;Spermatogenesis SUBCELLULAR LOCATION: Nucleus Cytoplasm, perinuclear region Chromosome Cytoplasm Note=Colocalizes with cdc-48.1 to the perinuclear region in spermatocytes. Localizes to the nucleus during S phase in a cdc-48 and npl-4-dependent manner. " H3BV60,"PROTEIN NAMES: Transforming growth factor-beta receptor type 3-like protein (TGF-beta receptor type-3-like protein) (TGFR-3L) (Transforming growth factor-beta receptor type III-like protein) (TGF-beta receptor type III-like protein) ORGANISM: Homo sapiens (Human) FUNCTION: Expressed in gonadotrope cells, acts as an inhibin B coreceptor and regulates follicle-stimulating hormone (FSH) levels and female fertility. KEYWORDS: Alternative splicing;Cell membrane;Disulfide bond;Membrane;Reference proteome;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass membrane protein " H3JQW0,"PROTEIN NAMES: 2-oxo-Delta(3)-4,5,5-trimethylcyclopentenylacetyl-CoA monooxygenase (OTEMO) ((2,2,3-trimethyl-5-oxocyclopent-3-enyl)acetyl-CoA 1,5-monooxygenase) (MO2) PROTEIN FAMILY: FAD-binding monooxygenase family ORGANISM: Pseudomonas putida (Arthrobacter siderocapsulatus) SIMILARITY: Belongs to the FAD-binding monooxygenase family. FUNCTION: Involved in the degradation of (+)-camphor. Catalyzes the lactonization of 2-oxo-delta(3)-4,5, 5-trimethylcyclopentenylacetyl-CoA (OT-CoA), a key intermediate in the metabolism of camphor. 2-Oxocyclopentyl ethyl acetate is also a good substrate, as is 2-oxocyclohexyl ethyl acetate and methyl-substituted cyclohexanones, but free acid is a poor substrate. PATHWAY: Terpene metabolism; (R)-camphor degradation. KEYWORDS: 3D-structure;FAD;Flavoprotein;Monooxygenase;NADP;Oxidoreductase;Plasmid " H8F0D7,PROTEIN NAMES: 2-methylcitrate synthase (2-MCS) (MCS) (Citrate synthase) (CS) PROTEIN FAMILY: Citrate synthase family ORGANISM: Mycobacterium tuberculosis (strain ATCC 35801 / TMC 107 / Erdman) SIMILARITY: Belongs to the citrate synthase family. FUNCTION: Involved in the catabolism of short chain fatty acids (SCFA) via the tricarboxylic acid (TCA)(acetyl degradation route) and via the 2-methylcitrate cycle I (propionate degradation route). Catalyzes the Claisen condensation of propionyl-CoA and oxaloacetate (OAA) to yield 2-methylcitrate (2-MC) and CoA. Also catalyzes the condensation of oxaloacetate with acetyl-CoA. PATHWAY: Organic acid metabolism; propanoate degradation.; PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 1/2. KEYWORDS: Reference proteome;Transferase;Tricarboxylic acid cycle H8ZPX1,PROTEIN NAMES: Pseudooxynicotine dehydrogenase (Pseudooxynicotine amine oxidase) (PNAO) PROTEIN FAMILY: Flavin monoamine oxidase family ORGANISM: Pseudomonas sp SIMILARITY: Belongs to the flavin monoamine oxidase family. FUNCTION: Involved in nicotine degradation. Catalyzes the deamination of pseudooxynicotine to 3-succinoylsemialdehyde-pyridine. PATHWAY: Alkaloid degradation; nicotine degradation. KEYWORDS: Alkaloid metabolism;FAD;Flavoprotein;Oxidoreductase;Periplasm;Signal SUBCELLULAR LOCATION: Periplasm H9JW43,"PROTEIN NAMES: Chitin deacetylase 8 (BmCDA8) (Chitin deacetylase 17) PROTEIN FAMILY: Carbohydrate esterase 4 (CE4) family ORGANISM: Bombyx mori (Silk moth) SIMILARITY: Belongs to the carbohydrate esterase 4 (CE4) family. FUNCTION: Hydrolyzes the N-acetamido groups of N-acetyl-D-glucosamine (GlcNAc) residues in chitin. Shows activity towards the chitinous oligomers GlcNAc(3), GlcNAc(4), GlcNAc(5) and GlcNAc(6), but not GlcNAc or GlcNAc(2). Requires the substrate to occupy subsites 0, +1, and +2 for optimum catalysis. KEYWORDS: 3D-structure;Carbohydrate metabolism;Chitin degradation;Disulfide bond;Glycoprotein;Hydrolase;Metal-binding;Polysaccharide degradation;Reference proteome;Secreted;Signal;Zinc SUBCELLULAR LOCATION: Secreted " I1GTC2,"PROTEIN NAMES: Probable 2' cyclic ADP-D-ribose synthase BdTIR (Probable 2'cADPR synthase AbTIR) (NAD(+) hydrolase TIR) (NADP(+) hydrolase TIR) (TIR-only protein) (BdTIR) ORGANISM: Brachypodium distachyon (Purple false brome) (Trachynia distachya) FUNCTION: An NAD(+) hydrolase (NADase). Upon activation catalyzes cleavage of NAD(+) into ADP-D-ribose (ADPR) and nicotinamide; NAD(+) cleavage triggers a defense system that promotes cell death. In addition to ADPR, also generates a cyclization variant of cyclic ADPR termed v-cADPR (2'cADPR). Also hydrolyzes NADP(+), but not other NAD(+)-related molecules. v-cADPR activates ThsA, an NAD(+) hydrolase in B.cereus (AC J8G6Z1). Probably makes 2'cADPR; the cADPR made by this protein is bound by cmTad1 (AC P0DW61) and activates ThsA from B.cereus. Boiling cmTad1 bound to the cyclic nucleotide releases 2'cADPR, strongly suggesting it is the product of this protein (Probable). KEYWORDS: Hydrolase;NAD;Plant defense;Reference proteome " I1SB12,"PROTEIN NAMES: Carbohydrate oxidase (Lactose oxidase) (LaO) PROTEIN FAMILY: Oxygen-dependent FAD-linked oxidoreductase family ORGANISM: Microdochium nivale (Pink snow mold) (Lanosa nivalis) SIMILARITY: Belongs to the oxygen-dependent FAD-linked oxidoreductase family. FUNCTION: Catalyzes the selective oxidation of C1 hydroxyl moieties on mono-, oligo- and polysaccharides with concomitant reduction of molecular oxygen to hydrogen peroxide. This results in the formation of the corresponding lactones, which typically undergo spontaneous hydrolysis. Carbohydrate oxidase is able to oxidize a variety of substrates including D-glucose, D-galactose, D-xylose, D-maltose, D-cellobiose, and lactose. In addition, among various oligosaccharides, the enzyme preferred tetrameric dextrins, indicating a favorable interaction of four linked glucose units with the substrate binding pocket. KEYWORDS: 3D-structure;Direct protein sequencing;FAD;Flavoprotein;Glycoprotein;Metal-binding;Nucleotide-binding;Oxidoreductase;Secreted;Signal;Zinc SUBCELLULAR LOCATION: Secreted " I1TEM1,"PROTEIN NAMES: Tabersonine 3-oxygenase (T3O) (16-methoxytabersonine 3-oxygenase) (Cytochrome P450 71D1) (Cytochrome P450 71D1V2) PROTEIN FAMILY: Cytochrome P450 family ORGANISM: Catharanthus roseus (Madagascar periwinkle) (Vinca rosea) SIMILARITY: Belongs to the cytochrome P450 family. FUNCTION: Cytochrome P450 catalyzing the monooxygenation of 16-methoxytabersonine, 16-hydroxytabersonine and tabersonine, but not of 2,3-dihydrotabersonine. Converts the C2,C3 alkene of tabersonine and 16-methoxytabersonine to the epoxides, which then spontaneously open to form the corresponding imine alcohols. Inactive in converting amyrin to ursolic acid. PATHWAY: Alkaloid biosynthesis; vindoline biosynthesis. KEYWORDS: Alkaloid metabolism;Endoplasmic reticulum;Heme;Iron;Membrane;Metal-binding;Monooxygenase;Oxidoreductase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Single-pass membrane protein. MISCELLANEOUS: The CYP71D1V2 product is either trapped by the reductase T3R to form vindorosine and vindoline precursors, or it spontaneously rearranges to form a member of the eburnamine class of monoterpene indole alkaloids." I2CYZ4,"PROTEIN NAMES: Acyl-lipid (7-3)-desaturase, chloroplastic (Acyl-lipid 4-desaturase) (Fatty acyl delta4 desaturase) PROTEIN FAMILY: Fatty acid desaturase type 1 family ORGANISM: Chlamydomonas reinhardtii (Chlamydomonas smithii) SIMILARITY: Belongs to the fatty acid desaturase type 1 family. FUNCTION: Fatty acid desaturase that introduces a cis double bond at the 4-position in 16-carbon polyunsaturated fatty acids that contain a Delta(7) double bond, resulting in the production of 16 carbon fatty acid (7Z,10Z,13Z)-hexadeca-7,10,13-trienoate. KEYWORDS: Chloroplast;Electron transport;Fatty acid biosynthesis;Fatty acid metabolism;Heme;Iron;Lipid biosynthesis;Lipid metabolism;Membrane;Metal-binding;Oxidoreductase;Plastid;Transit peptide;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Plastid, chloroplast membrane ; Multi-pass membrane protein " I3R634,"PROTEIN NAMES: Assimilatory nitrate reductase PROTEIN FAMILY: Prokaryotic molybdopterin-containing oxidoreductase family, NasA/NapA/NarB subfamily ORGANISM: Haloferax mediterranei (strain ATCC 33500 / DSM 1411 / JCM 8866 / NBRC 14739 / NCIMB 2177 / R-4) (Halobacterium mediterranei) SIMILARITY: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily. FUNCTION: Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation. Catalyzes the reduction of nitrate to nitrite, using ferredoxin as the electron donor. Can use reduced methyl viologen but neither NADPH nor NADH as electron donors. PATHWAY: Nitrogen metabolism; nitrate reduction (assimilation). KEYWORDS: 4Fe-4S;Cytoplasm;Iron;Iron-sulfur;Metal-binding;Molybdenum;Nitrate assimilation;Oxidoreductase SUBCELLULAR LOCATION: Cytoplasm MISCELLANEOUS: Enzyme stability and activity depend upon the salt concentration." I3R637,"PROTEIN NAMES: Assimilatory ferredoxin-dependent nitrite reductase (NiR) (Ferredoxin:nitrite reductase) PROTEIN FAMILY: Nitrite and sulfite reductase 4Fe-4S domain family ORGANISM: Haloferax mediterranei (strain ATCC 33500 / DSM 1411 / JCM 8866 / NBRC 14739 / NCIMB 2177 / R-4) (Halobacterium mediterranei) SIMILARITY: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family. FUNCTION: Catalyzes the reduction of nitrite to ammonium in the nitrate assimilation pathway, using ferredoxin as the electron donor. Can use reduced methyl viologen but neither NADPH nor NADH as electron donors. PATHWAY: Nitrogen metabolism; nitrate reduction (assimilation). KEYWORDS: 4Fe-4S;Electron transport;Heme;Iron;Iron-sulfur;Metal-binding;Nitrate assimilation;Oxidoreductase;Transport MISCELLANEOUS: Enzyme stability and activity depend upon the salt concentration." I3R9M8,PROTEIN NAMES: Respiratory nitrate reductase subunit beta (Nitrate reductase beta chain) ORGANISM: Haloferax mediterranei (strain ATCC 33500 / DSM 1411 / JCM 8866 / NBRC 14739 / NCIMB 2177 / R-4) (Halobacterium mediterranei) FUNCTION: The respiratory membrane-bound nitrate reductase enzyme complex plays a role in generation of metabolic energy by using nitrate as a terminal electron acceptor during anaerobic conditions. The beta chain is an electron transfer unit containing four cysteine clusters involved in the formation of iron-sulfur centers. KEYWORDS: 4Fe-4S;Cell membrane;Electron transport;Iron;Iron-sulfur;Membrane;Metal-binding;Nitrate assimilation;Oxidoreductase;Plasmid;Repeat;Transport SUBCELLULAR LOCATION: Cell membrane ; Peripheral membrane protein ; Extracellular side Note=Proposed to be coexported with the Tat system-dependent alpha subunit. I3R9M9,PROTEIN NAMES: Respiratory nitrate reductase subunit alpha (Nitrate reductase alpha chain) PROTEIN FAMILY: Prokaryotic molybdopterin-containing oxidoreductase family ORGANISM: Haloferax mediterranei (strain ATCC 33500 / DSM 1411 / JCM 8866 / NBRC 14739 / NCIMB 2177 / R-4) (Halobacterium mediterranei) SIMILARITY: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. FUNCTION: The respiratory membrane-bound nitrate reductase enzyme complex plays a role in generation of metabolic energy by using nitrate as a terminal electron acceptor during anaerobic conditions. The alpha chain is the actual site of nitrate reduction. KEYWORDS: 4Fe-4S;Cell membrane;Electron transport;Iron;Iron-sulfur;Membrane;Metal-binding;Molybdenum;Nitrate assimilation;Oxidoreductase;Plasmid;Signal;Transport SUBCELLULAR LOCATION: Cell membrane ; Peripheral membrane protein ; Extracellular side I3V6A7,"PROTEIN NAMES: Scoulerine-9-O-methyltransferase 1 (PsSOMT1) (Norreticuline 3-O-methyltransferase SOMT1) (O-methyltransferase 1) (Reticuline 3-O-methyltransferase SOMT1) (Tetrahydrocolumbamine 2-O-methyltransferase SOMT1) PROTEIN FAMILY: Class I-like SAM-binding methyltransferase superfamily, Cation-independent O-methyltransferase family, COMT subfamily ORGANISM: Papaver somniferum (Opium poppy) SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O-methyltransferase family. COMT subfamily. FUNCTION: Methyltransferase involved in the biosynthesis of the benzylisoquinoline alkaloid noscapine. Catalyzes the conversion of (S)-scoulerine to (S)-tetrahydrocolumbamine. Can convert (S)-tetrahydrocolumbamine to tetrahydropalmatine. Can convert (S)-norreticuline to (S)-norcodamine. Can convert (S)-reticuline to (S)-codamine. Substrate preference is (S)-scoulerine > (S)-tetrahydrocolumbamine > (S)-norreticuline > (S)-reticuline. PATHWAY: Alkaloid biosynthesis. KEYWORDS: 3D-structure;Alkaloid metabolism;Methyltransferase;S-adenosyl-L-methionine;Transferase " I6WXK4,"PROTEIN NAMES: Triple specificity protein phosphatase PtpB (MPtpB) (Phosphoinositide phosphatase) (Protein-serine/threonine phosphatase) (Protein-tyrosine phosphatase B) (TSP PTP) PROTEIN FAMILY: Protein-tyrosine phosphatase family ORGANISM: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) SIMILARITY: Belongs to the protein-tyrosine phosphatase family. FUNCTION: Essential virulence factor that promotes mycobacterial survival within host macrophages. Acts as a phosphatase that possesses triple substrate specificity toward phosphotyrosine, phosphoserine/threonine and phosphoinositides. Supports mycobacteria survival during infection by modulating the normal host signaling pathways, attenuating the bactericidal immune responses and promoting the host cell survival. Inhibits host pyroptosis by disrupting the membrane localization of host gasdermin-D (GSDMD): acts by catalyzing dephosphorylation of phosphatidylinositol (4,5)-bisphosphate and phosphatidylinositol 4-phosphate, thereby inhibiting the membrane targeting of GSDMD and subsequent cytokine release and pyroptosis. Inhibits host inflammatory responses and apoptosis through impeding the NF-kappa-B and MAPK signal pathways and TP53/p53 expression in the macrophage. Blocks the IL6/IL-6 production by down-regulating ERK1/2, p38 and p65 activity. Prevents macrophage cell death by activating the Akt pathway and blocking caspase 3 activity. Reduces the expression of iNOS in activated macrophages and inhibits the generation of destroying reactive nitrogen intermediate NO. KEYWORDS: 3D-structure;Host cell membrane;Host cytoplasm;Host membrane;Hydrolase;Membrane;Protein phosphatase;Reference proteome;Secreted;Virulence SUBCELLULAR LOCATION: Secreted Host cytoplasm Host cell membrane Note=Further investigation is required to determine whether PtpB is direct substrate for SecA2, the ESX/type VII secretion system, or an alternate mechanism. " I6XHI4,"PROTEIN NAMES: Steroid 3-ketoacyl-CoA thiolase (Acetyl-CoA acetyltransferase FadA5) (Beta-ketoacyl-CoA thiolase) PROTEIN FAMILY: Thiolase-like superfamily, Thiolase family ORGANISM: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) SIMILARITY: Belongs to the thiolase-like superfamily. Thiolase family. FUNCTION: Involved in the beta-oxidation of the cholesterol side chain. It is important for utilization of cholesterol as a sole carbon source in vitro and for full virulence in the chronic stage of mouse lung infection. Catalyzes the thiolysis of 3,22-dioxochol-4-en-24-oyl-CoA to yield 3-oxo-4-pregnene-20-carboxyl-CoA (3-OPC-CoA) and acetyl-CoA. Also able to use acetoacetyl-CoA (AcAcCoA) as substrate. PATHWAY: Steroid metabolism; cholesterol degradation. KEYWORDS: 3D-structure;Acyltransferase;Cholesterol metabolism;Lipid degradation;Lipid metabolism;Reference proteome;Steroid metabolism;Sterol metabolism;Transferase;Virulence " I6Y0R5,"PROTEIN NAMES: Dihydrofolate synthase/folylpolyglutamate synthase (DHFS / FPGS) (Folylpoly-gamma-glutamate synthetase) (Tetrahydrofolylpolyglutamate synthase) PROTEIN FAMILY: Folylpolyglutamate synthase family ORGANISM: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) SIMILARITY: Belongs to the folylpolyglutamate synthase family. FUNCTION: Catalyzes the addition of a glutamate residue to dihydropteroate (7,8-dihydropteroate or H2Pte) to form dihydrofolate (7,8-dihydrofolate monoglutamate or H2Pte-Glu). Also catalyzes successive additions of L-glutamate to tetrahydrofolate, leading to folylpolyglutamate derivatives (By similarity).; FUNCTION: Is involved in the bioactivation of the antituberculous drug para-aminosalicylic acid (PAS). Is able to use hydroxy-dihydropteroate (H2PtePAS) as substrate, which is the product formed by the action of DHPS (FolP1) on PAS, leading to hydroxy-dihydrofolate (H2PtePAS-Glu). This compound inhibits dihydrofolate reductase DHFR (DfrA), the next enzyme in the folate pathway, and thus disrupts the folate-dependent metabolic pathways. PATHWAY: Cofactor biosynthesis; tetrahydrofolate biosynthesis; 7,8-dihydrofolate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate and 4-aminobenzoate: step 2/2.; PATHWAY: Cofactor biosynthesis; tetrahydrofolylpolyglutamate biosynthesis. KEYWORDS: 3D-structure;Antibiotic resistance;ATP-binding;Folate biosynthesis;Ligase;Magnesium;Metal-binding;Nucleotide-binding;Reference proteome MISCELLANEOUS: Mutations within this gene in the H2Pte binding pocket are responsible for PAS resistance in M.tuberculosis clinical isolates and laboratory strains. FolC-linked PAS resistance is mediated by altered substrate specificity that results in the failure to generate levels of H2PtePAS-Glu that are necessary to inhibit DHFR (DfrA); therefore, a blockage of PAS bioactivation causes PAS resistance." I6Y778,PROTEIN NAMES: 3-oxoacyl-[acyl-carrier-protein] reductase [NADH] (Beta-ketoacyl CoA reductase) (FASII-like 3-oxoacyl-thioester reductase) (HMwFabG) PROTEIN FAMILY: Short-chain dehydrogenases/reductases (SDR) family ORGANISM: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FUNCTION: Catalyzes the NADH-dependent reduction of beta-ketoacyl derivatives. Can accept the beta-oxo fatty acyl group covalently linked with CoA or ACP for catalysis. Highly specific for NADH. Could be involved in fatty acid biosynthesis (Probable). PATHWAY: Lipid metabolism; fatty acid metabolism. KEYWORDS: 3D-structure;Fatty acid metabolism;Lipid metabolism;NAD;Oxidoreductase;Reference proteome MISCELLANEOUS: Restores respiratory growth of S.cerevisiae oar1 deletion mutant.; MISCELLANEOUS: Was identified as a putative drug target. Inhibited by triazole linked polyphenol-gallol hybrids and triazole linked polyphenol-aminobenzene hybrids. These compounds may be possible candidates for alternate anti-tubercular drugs. J3QMY9,"PROTEIN NAMES: Type 2 DNA topoisomerase 6 subunit B-like (TOP6B like initiator of meiotic double strand breaks) (Type 2 DNA topoisomerase VI subunit B-like) (TOPOVIBL) PROTEIN FAMILY: TOP6B-like family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the TOP6B-like family. FUNCTION: Component of a topoisomerase 6 complex specifically required for meiotic recombination. Together with SPO11, mediates DNA cleavage that forms the double-strand breaks (DSB) that initiate meiotic recombination. The complex promotes relaxation of negative and positive supercoiled DNA and DNA decatenation through cleavage and ligation cycles. KEYWORDS: 3D-structure;Alternative splicing;Chromosome;Meiosis;Reference proteome SUBCELLULAR LOCATION: Chromosome Note=Localizes to meiotic chromosomes. " J7I4B7,"PROTEIN NAMES: O-antigen chain terminator bifunctional methyltransferase/kinase WbdD [Includes: 3-O-phospho-polymannosyl GlcNAc-diphospho-ditrans,octacis-undecaprenol 3-phospho-methyltransferase ; Polymannosyl GlcNAc-diphospho-ditrans,octacis-undecaprenol kinase ] PROTEIN FAMILY: WbdD family ORGANISM: Escherichia coli SIMILARITY: Belongs to the WbdD family. FUNCTION: Regulates the length of the LPS O-antigen polysaccharide chain. Stops the polymerization of the chain by phosphorylating and then methylating the phosphate on the terminal sugar. This terminal modification is essential for export of the O-antigen across the inner membrane. WbdD is also required for correct localization of the WbdA mannosyltransferase. PATHWAY: Bacterial outer membrane biogenesis; LPS O-antigen biosynthesis. KEYWORDS: 3D-structure;ATP-binding;Cell inner membrane;Cell membrane;Coiled coil;Kinase;Lipopolysaccharide biosynthesis;Membrane;Methyltransferase;Nucleotide-binding;S-adenosyl-L-methionine;Transferase SUBCELLULAR LOCATION: Cell inner membrane ; Peripheral membrane protein ; Cytoplasmic side " J9VP29,PROTEIN NAMES: Mitochondrial respiration co-chaperone MRJ1 (Mitochondrial respiration J-domain protein 1) PROTEIN FAMILY: DnaJ family ORGANISM: Cryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487) (Filobasidiella neoformans var. grubii) SIMILARITY: Belongs to the DnaJ family. FUNCTION: Mitochondrial co-chaperone required for ubiquinol-cytochrome c oxidoreductase (mitochondrial respiratory chain complex III) activity. KEYWORDS: Chaperone;Electron transport;Mitochondrion;Respiratory chain;Transit peptide;Transport SUBCELLULAR LOCATION: Mitochondrion K4L7X3,"PROTEIN NAMES: 3-sulfinopropanoyl-CoA desulfinase (3-sulfinopropionyl coenzyme A desulfinase) (3-sulfinopropionyl-CoA desulfinase) (3SP-CoA desulfinase) PROTEIN FAMILY: Acyl-CoA dehydrogenase family ORGANISM: Advenella mimigardefordensis (strain DSM 17166 / LMG 22922 / DPN7) SIMILARITY: Belongs to the acyl-CoA dehydrogenase family. FUNCTION: Catalyzes the conversion 3-sulfinopropanoyl-CoA (3SP-CoA) to propanoyl-CoA by abstraction of sulfite. Does not show dehydrogenase activity. Involved in the degradation of 3,3'-dithiodipropionate (DTDP), a sulfur-containing precursor substrate for biosynthesis of polythioesters (PTEs). KEYWORDS: 3D-structure;FAD;Flavoprotein;Hydrolase;Reference proteome MISCELLANEOUS: Does not catalyze the dehydrogenation of acyl-CoA thioesters. The absence of dehydrogenase activity is most probably caused by the absence of a catalytic glutamate residue in either of the two positions conserved throughout the acyl-CoA dehydrogenase family." K4REZ6,"PROTEIN NAMES: 8-demethyl-8-aminoriboflavin-5'-phosphate synthase (AFP synthase) (8-amino-flavin synthase) PROTEIN FAMILY: SsuE family ORGANISM: Streptomyces davaonensis (strain DSM 101723 / JCM 4913 / KCC S-0913 / 768) SIMILARITY: Belongs to the SsuE family. FUNCTION: Involved in the biosynthesis of the riboflavin analog antibiotic roseoflavin (3,8-dimethylamino-riboflavin). Catalyzes the site-specific substitution of the C-8 methyl group of riboflavin-5'-phosphate (FMN) by an amino group to yield 8-amino-8-demethylriboflavin 5'-phosphate, via a combined oxidation, decarboxylation and transamination reaction. The catalysis is initiated by an oxidation step in which the C-8 methyl group on the dimethylbenzene ring of FMN is converted to a formyl group to yield the 8-demethyl-8-formylriboflavin-5'-phosphate (OHC-RP) intermediate. In the presence of thiamine, the formyl group is oxidized into a carboxyl group to yield the 8-demethyl-8-carboxyriboflavin-5'-phosphate (HO2C-RP) intermediate. Finally, in the presence of L-glutamate as an amino donor, decarboxylation and aminotransfer occur, resulting in production of 8-demethyl-8-aminoriboflavin-5'-phosphate. Addition of NAD (but not NADP) to the reaction increases the yield 1.7-fold. The reaction also proceeds without the addition of any electron acceptor, and it is possible that molecular oxygen serves this role. PATHWAY: Antibiotic biosynthesis. KEYWORDS: 3D-structure;Antibiotic biosynthesis;Flavoprotein;FMN;Reference proteome;Transferase " K4ZRC1,"PROTEIN NAMES: Pro-Pro endopeptidase (PPEP-2) PROTEIN FAMILY: Peptidase M34 family, Pro-Pro endopeptidase subfamily ORGANISM: Paenibacillus alvei (strain ATCC 6344 / DSM 29 / NBRC 3343 / NCIMB 9371 / NCTC 6352) (Bacillus alvei) SIMILARITY: Belongs to the peptidase M34 family. Pro-Pro endopeptidase subfamily. FUNCTION: Zinc-dependent endoprotease with a unique preference for proline residues surrounding the scissile bond, which cleaves in a PLP-|-PVP motif. Cleaves the cell surface protein encoded by an adjacent gene, which contains two PPEP-2 cleaving sites and putative extracellular matrix-binding domains. Thereby, may have a role in the regulation of P.alvei adhesion. Is not able to cleave within the PVP-|-PVQ motif, and only shows a very poor cleavage of the VNP-|-PVP motif in vitro, which is the optimal substrate peptide for PPEP-1 from P.difficile. KEYWORDS: 3D-structure;Hydrolase;Metal-binding;Metalloprotease;Protease;Secreted;Signal;Zinc SUBCELLULAR LOCATION: Secreted " K7QRJ5,"PROTEIN NAMES: Dialkyldecalin synthase (FAD-dependent [4+2] cyclase) PROTEIN FAMILY: PheA/TfdB FAD monooxygenase family ORGANISM: Streptomyces rugosporus SIMILARITY: Belongs to the PheA/TfdB FAD monooxygenase family. FUNCTION: Involved in the biosynthesis of the spirotetramate antibiotics pyrroindomycins. Catalyzes the intramolecular cyclization forming the dialkyldecalin moiety in pyrroindomycins, via an endo-selective [4+2] cycloaddition reaction. PATHWAY: Antibiotic biosynthesis. KEYWORDS: 3D-structure;Antibiotic biosynthesis;FAD;Flavoprotein;Isomerase " K7QVW7,"PROTEIN NAMES: Spiro-conjugate synthase ([4+2] cyclase PyrI4) ORGANISM: Streptomyces rugosporus FUNCTION: Involved in the biosynthesis of the spirotetramate antibiotics pyrroindomycins. Catalyzes the intramolecular cyclization forming the spiro-conjugate moiety in pyrroindomycins, via an exo-selective [4+2] cycloaddition reaction. PATHWAY: Antibiotic biosynthesis. KEYWORDS: 3D-structure;Antibiotic biosynthesis;Disulfide bond;Isomerase MISCELLANEOUS: Reaction occurs via a unique trapping mechanism whereby the lid-like action of the N-terminal tail imposes conformational constraints on the beta-barrel catalytic core, which enhances the proximity and polarization effects of reactive groups (1,3-diene and alkene) to drive cyclization in a regio- and stereo-specific manner." K7WIZ6,"PROTEIN NAMES: Riboflavin biosynthesis protein PYRR, chloroplastic [Includes: Inactive diaminohydroxyphosphoribosylaminopyrimidine deaminase (DRAP deaminase) (Riboflavin-specific deaminase); 5-amino-6-(5-phosphoribosylamino)uracil reductase (HTP reductase); Riboflavin biosynthesis intermediates N-glycosidase ] PROTEIN FAMILY: YbiA family ORGANISM: Zea mays (Maize) SIMILARITY: In the C-terminal section; belongs to the YbiA family. FUNCTION: Pyrimidine reductase involved in the riboflavin biosynthesis pathway. Has also a non-functional N-terminal deaminase domain that lacks the catalytically essential zinc-binding residues. 39% activity when NADH replaces NADPH. No evidence for a phosphatase activity conferred by the N-terminal domain.; FUNCTION: Catalyzes the hydrolysis of the N-glycosidic bond in the first two intermediates of riboflavin biosynthesis, which are highly reactive metabolites, yielding relatively innocuous products. Thus, can divert a surplus of harmful intermediates into relatively harmless products and pre-empt the damage these intermediates would otherwise do. Has no activity against GTP, nucleoside monophosphates or ADP-ribose. PATHWAY: Cofactor biosynthesis; riboflavin biosynthesis; 5-amino-6-(D-ribitylamino)uracil from GTP: step 3/4. KEYWORDS: Chloroplast;Glycosidase;Hydrolase;Multifunctional enzyme;NADP;Oxidoreductase;Plastid;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast MISCELLANEOUS: Unlike bacteria that have a bifunctional, two-domain RibD enzyme, plants have a monofunctional reductase and a monofunctional deaminase, each having an enzymatically inactive domain." L0DSL2,"PROTEIN NAMES: Cytochrome c-552 (Cytochrome c nitrite reductase) (TvNiR) PROTEIN FAMILY: Cytochrome c-552 family ORGANISM: Thioalkalivibrio nitratireducens (strain DSM 14787 / UNIQEM 213 / ALEN2) SIMILARITY: Belongs to the cytochrome c-552 family. FUNCTION: Catalyzes the reduction of nitrite to ammonia, consuming six electrons in the process. Has very low activity toward hydroxylamine. Has even lower activity toward sulfite. Sulfite reductase activity is maximal at neutral pH. PATHWAY: Nitrogen metabolism; nitrate reduction (assimilation). KEYWORDS: 3D-structure;Calcium;Direct protein sequencing;Electron transport;Heme;Iron;Metal-binding;Oxidoreductase;Periplasm;Signal;Thioether bond;Transport SUBCELLULAR LOCATION: Periplasm. " L0E155,"PROTEIN NAMES: Flavin-dependent halogenase malA (Malbrancheamide biosynthesis cluster protein A) PROTEIN FAMILY: Flavin-dependent halogenase family ORGANISM: Malbranchea aurantiaca SIMILARITY: Belongs to the flavin-dependent halogenase family. FUNCTION: Flavin-dependent halogenase; part of the gene cluster that mediates the biosynthesis of malbrancheamide, a dichlorinated fungal indole alkaloid that belongs to a family of natural products containing a characteristic bicyclo[2.2.2]diazaoctane core. The first step of malbrancheamide biosynthesis involves coupling of L-proline and L-tryptophan by malG, a bimodular NRPS, to produce L-Pro-L-Trp aldehyde through reductive offloading. This compound undergoes spontaneous cyclization and dehydration to give a dienamine which is reverse prenylated at C-2 by malE. The other prenyltransferase present in the cluster, malB, displays modest activity, suggesting that may be a redundant gene in the pathway. Subsequently, a [4+2] Diels-Alder cyclo-addition catalyzed by the bifunctional enzyme malC forms the characteristic bicyclo[2.2.2]diazaoctane ring of premalbrancheamid. Finally, the flavin-dependent halogenase malA catalyzes the iterative dichlorination of the indole ring of premalbrancheamide to yield C-9 monochlorinated malbrancheamide B, C-8 monochlorinated isomalbrancheamide B, and dichlorinated malbrancheamide. MalA is also able to brominate premalbrancheamide at C-9 to yield malbrancheamide C, and, to a lesser extend, at C-8 to yield isomalbrancheamide C. Finally, malA can brominate C-9 monochlorinated malbrancheamide B at C-8 to yield malbrancheamide D, or C-8 monochlorinated isomalbrancheamide B at C-9 to produce isomalbrancheamide D. PATHWAY: Alkaloid biosynthesis. KEYWORDS: 3D-structure;FAD;Flavoprotein;Monooxygenase;Oxidoreductase;Zinc " L7N1X6,"PROTEIN NAMES: F-box/WD repeat-containing protein 15 ORGANISM: Mus musculus (Mouse) FUNCTION: Substrate-recognition component of an SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. Promotes KAT7 ubiquitination and subsequent degradation in collaboration with MAP2K1 kinase, leading to reduced histone H3K14 acetylation and increased cell proliferation. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Alternative splicing;Cytoplasm;Endoplasmic reticulum;Nucleus;Reference proteome;Repeat;Ubl conjugation pathway;WD repeat SUBCELLULAR LOCATION: Cytoplasm, cytosol Endoplasmic reticulum Nucleus " L7YAI7,"PROTEIN NAMES: Beta-1,4-glucuronyltransferase 1 (I-beta-1,3-N-acetylglucosaminyltransferase) (N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase) (Poly-N-acetyllactosamine extension enzyme) (UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 1) PROTEIN FAMILY: Glycosyltransferase 49 family ORGANISM: Danio rerio (Zebrafish) (Brachydanio rerio) SIMILARITY: Belongs to the glycosyltransferase 49 family. FUNCTION: Beta-1,4-glucuronyltransferase involved in O-mannosylation of alpha-dystroglycan (DAG1). Transfers a glucuronic acid (GlcA) residue onto a xylose (Xyl) acceptor to produce the glucuronyl-beta-1,4-xylose-beta disaccharide primer, which is further elongated by LARGE, during synthesis of phosphorylated O-mannosyl glycan (By similarity). Phosphorylated O-mannosyl glycan is a carbohydrate structure present in alpha-dystroglycan (DAG1), which is required for binding laminin G-like domain-containing extracellular proteins with high affinity (By similarity). Required for axon guidance; via its function in O-mannosylation of alpha-dystroglycan (DAG1) (By similarity). PATHWAY: Protein modification; protein glycosylation. KEYWORDS: Glycoprotein;Glycosyltransferase;Golgi apparatus;Manganese;Membrane;Metal-binding;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus membrane ; Single-pass type II membrane protein. Note=Localizes near the trans-Golgi apparatus. " M0R7Z9,"PROTEIN NAMES: Perilipin-5 (Lipid storage droplet protein 5) PROTEIN FAMILY: Perilipin family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the perilipin family. FUNCTION: Lipid droplet-associated protein that maintains the balance between lipogenesis and lipolysis and also regulates fatty acid oxidation in oxidative tissues. Recruits mitochondria to the surface of lipid droplets and is involved in lipid droplet homeostasis by regulating both the storage of fatty acids in the form of triglycerides and the release of fatty acids for mitochondrial fatty acid oxidation. In lipid droplet triacylglycerol hydrolysis, plays a role as a scaffolding protein for three major key lipolytic players: ABHD5, PNPLA2 and LIPE. Reduces the triacylglycerol hydrolase activity of PNPLA2 by recruiting and sequestering PNPLA2 to lipid droplets. Phosphorylation by PKA enables lipolysis probably by promoting release of ABHD5 from the perilipin scaffold and by facilitating interaction of ABHD5 with PNPLA2. Also increases lipolysis through interaction with LIPE and upon PKA-mediated phosphorylation of LIPE. KEYWORDS: Cytoplasm;Lipid droplet;Mitochondrion;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Lipid droplet Cytoplasm Mitochondrion Note=Lipid droplet surface-associated. Exchanges between lipid droplets and the cytoplasm. " M9PBE2,"PROTEIN NAMES: E3 ubiquitin-protein ligase Hakai PROTEIN FAMILY: Hakai family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the Hakai family. FUNCTION: E3 ubiquitin-protein ligase required during early development. E3 ubiquitin-protein ligases mediate ubiquitination of target proteins. Required for epithelial integrity and midgut morphogenesis. Associated component of the WMM complex, a complex that mediates N6-methyladenosine (m6A) methylation of RNAs, a modification that plays a role in the efficiency of mRNA splicing and RNA processing. Its function in the WMM complex is unknown. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Alternative splicing;Cell membrane;Cytoplasm;Cytoplasmic vesicle;Developmental protein;Membrane;Metal-binding;Nucleus;Reference proteome;Transferase;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Cell membrane Cytoplasmic vesicle Cytoplasm, perinuclear region " O00116,"PROTEIN NAMES: Alkyldihydroxyacetonephosphate synthase, peroxisomal (Alkyl-DHAP synthase) (Aging-associated gene 5 protein) (Alkylglycerone-phosphate synthase) PROTEIN FAMILY: FAD-binding oxidoreductase/transferase type 4 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the FAD-binding oxidoreductase/transferase type 4 family. FUNCTION: Catalyzes the exchange of the acyl chain in acyl-dihydroxyacetonephosphate (acyl-DHAP) for a long chain fatty alcohol, yielding the first ether linked intermediate, i.e. alkyl-dihydroxyacetonephosphate (alkyl-DHAP), in the pathway of ether lipid biosynthesis. PATHWAY: Glycerolipid metabolism; ether lipid biosynthesis. KEYWORDS: Acetylation;Cataract;Disease variant;Dwarfism;FAD;Flavoprotein;Lipid biosynthesis;Lipid metabolism;Membrane;Peroxisome;Phosphoprotein;Reference proteome;Rhizomelic chondrodysplasia punctata;Transferase;Transit peptide SUBCELLULAR LOCATION: Peroxisome membrane Peroxisome " O00189,"PROTEIN NAMES: AP-4 complex subunit mu-1 (AP-4 adaptor complex mu subunit) (Adaptor-related protein complex 4 subunit mu-1) (Mu subunit of AP-4) (Mu-adaptin-related protein 2) (mu-ARP2) (Mu4-adaptin) (mu4) PROTEIN FAMILY: Adaptor complexes medium subunit family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the adaptor complexes medium subunit family. FUNCTION: Component of the adaptor protein complex 4 (AP-4). Adaptor protein complexes are vesicle coat components involved both in vesicle formation and cargo selection. They control the vesicular transport of proteins in different trafficking pathways. AP-4 forms a non clathrin-associated coat on vesicles departing the trans-Golgi network (TGN) and may be involved in the targeting of proteins from the trans-Golgi network (TGN) to the endosomal-lysosomal system. It is also involved in protein sorting to the basolateral membrane in epithelial cells and the proper asymmetric localization of somatodendritic proteins in neurons (By similarity). Within AP-4, the mu-type subunit AP4M1 is directly involved in the recognition and binding of tyrosine-based sorting signals found in the cytoplasmic part of cargos. The adaptor protein complex 4 (AP-4) may also recognize other types of sorting signal (By similarity). KEYWORDS: 3D-structure;Endosome;Golgi apparatus;Hereditary spastic paraplegia;Membrane;Neurodegeneration;Protein transport;Reference proteome;Transport SUBCELLULAR LOCATION: Golgi apparatus, trans-Golgi network membrane ; Peripheral membrane protein Early endosome Note=Found in soma and dendritic shafts of neuronal cells. " O00204,"PROTEIN NAMES: Sulfotransferase 2B1 (Alcohol sulfotransferase) (Hydroxysteroid sulfotransferase 2) (Sulfotransferase family 2B member 1) (Sulfotransferase family cytosolic 2B member 1) (ST2B1) PROTEIN FAMILY: Sulfotransferase 1 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the sulfotransferase 1 family. FUNCTION: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation. Responsible for the sulfation of cholesterol. Catalyzes sulfation of the 3beta-hydroxyl groups of steroids, such as, pregnenolone and dehydroepiandrosterone (DHEA). Preferentially sulfonates cholesterol, while it has also significant activity with pregnenolone and DHEA. Plays a role in epidermal cholesterol metabolism and in the regulation of epidermal proliferation and differentiation.; FUNCTION: [Isoform 2]: Sulfonates pregnenolone but not cholesterol. KEYWORDS: 3D-structure;Alternative splicing;Cytoplasm;Disease variant;Endoplasmic reticulum;Ichthyosis;Lipid metabolism;Microsome;Nucleus;Phosphoprotein;Reference proteome;Steroid metabolism;Transferase SUBCELLULAR LOCATION: Cytoplasm, cytosol Microsome Nucleus Note=Phosphorylation of Ser-348 is required for translocation to the nucleus. " O00232,"PROTEIN NAMES: 26S proteasome non-ATPase regulatory subunit 12 (26S proteasome regulatory subunit RPN5) (26S proteasome regulatory subunit p55) PROTEIN FAMILY: Proteasome subunit p55 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the proteasome subunit p55 family. FUNCTION: Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. KEYWORDS: 3D-structure;Acetylation;Alternative splicing;Direct protein sequencing;Intellectual disability;Isopeptide bond;Proteasome;Reference proteome;Ubl conjugation " O00273,PROTEIN NAMES: DNA fragmentation factor subunit alpha (DNA fragmentation factor 45 kDa subunit) (DFF-45) (Inhibitor of CAD) (ICAD) ORGANISM: Homo sapiens (Human) FUNCTION: Inhibitor of the caspase-activated DNase (DFF40). KEYWORDS: 3D-structure;Acetylation;Alternative splicing;Apoptosis;Cytoplasm;Direct protein sequencing;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cytoplasm. O00322,"PROTEIN NAMES: Uroplakin-1a (UP1a) (Tetraspanin-21) (Tspan-21) (Uroplakin Ia) (UPIa) (UPKa) PROTEIN FAMILY: Tetraspanin (TM4SF) family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the tetraspanin (TM4SF) family. FUNCTION: Component of the asymmetric unit membrane (AUM); a highly specialized biomembrane elaborated by terminally differentiated urothelial cells. May play an important role in normal bladder epithelial physiology, possibly in regulating membrane permeability of superficial umbrella cells or in stabilizing the apical membrane through AUM/cytoskeletal interactions (By similarity). KEYWORDS: Alternative splicing;Disulfide bond;Glycoprotein;Membrane;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. " O00339,PROTEIN NAMES: Matrilin-2 ORGANISM: Homo sapiens (Human) FUNCTION: Involved in matrix assembly. KEYWORDS: Alternative splicing;Coiled coil;Disulfide bond;EGF-like domain;Glycoprotein;Reference proteome;Repeat;Secreted;Signal SUBCELLULAR LOCATION: Secreted. O00401,"PROTEIN NAMES: Actin nucleation-promoting factor WASL (Neural Wiskott-Aldrich syndrome protein) (N-WASP) ORGANISM: Homo sapiens (Human) FUNCTION: Regulates actin polymerization by stimulating the actin-nucleating activity of the Arp2/3 complex. Involved in various processes, such as mitosis and cytokinesis, via its role in the regulation of actin polymerization. Together with CDC42, involved in the extension and maintenance of the formation of thin, actin-rich surface projections called filopodia. In addition to its role in the cytoplasm, also plays a role in the nucleus by regulating gene transcription, probably by promoting nuclear actin polymerization. Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression (By similarity). Plays a role in dendrite spine morphogenesis (By similarity). Decreasing levels of DNMBP (using antisense RNA) alters apical junction morphology in cultured enterocytes, junctions curve instead of being nearly linear. KEYWORDS: 3D-structure;Acetylation;Actin-binding;Cell cycle;Cell division;Cytoplasm;Cytoskeleton;Methylation;Mitosis;Nucleus;Phosphoprotein;Reference proteome;Repeat;Transcription;Transcription regulation SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton Nucleus Cytoplasm Note=Preferentially localized in the cytoplasm when phosphorylated and in the nucleus when unphosphorylated (By similarity). Exported from the nucleus by an nuclear export signal (NES)-dependent mechanism to the cytoplasm (By similarity). " O00418,"PROTEIN NAMES: Eukaryotic elongation factor 2 kinase (eEF-2 kinase) (eEF-2K) (Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase) PROTEIN FAMILY: Protein kinase superfamily, Alpha-type protein kinase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the protein kinase superfamily. Alpha-type protein kinase family. FUNCTION: Threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced. KEYWORDS: 3D-structure;Acetylation;ATP-binding;Calcium;Calmodulin-binding;Kinase;Nucleotide-binding;Phosphoprotein;Reference proteome;Serine/threonine-protein kinase;Transferase " O00442,"PROTEIN NAMES: RNA 3'-terminal phosphate cyclase (RNA cyclase) (RNA-3'-phosphate cyclase) (RNA terminal phosphate cyclase domain-containing protein 1) (RTC domain-containing protein 1) PROTEIN FAMILY: RNA 3'-terminal cyclase family, Type 1 subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily. FUNCTION: Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. Likely functions in some aspects of cellular RNA processing. Function plays an important role in regulating axon regeneration by inhibiting central nervous system (CNS) axon regeneration following optic nerve injury. KEYWORDS: Alternative splicing;ATP-binding;Direct protein sequencing;Ligase;Nucleotide-binding;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus, nucleoplasm " O00472,"PROTEIN NAMES: RNA polymerase II elongation factor ELL2 PROTEIN FAMILY: ELL/occludin family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the ELL/occludin family. FUNCTION: Elongation factor component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. Component of the little elongation complex (LEC), a complex required to regulate small nuclear RNA (snRNA) gene transcription by RNA polymerase II and III. Plays a role in immunoglobulin secretion in plasma cells: directs efficient alternative mRNA processing, influencing both proximal poly(A) site choice and exon skipping, as well as immunoglobulin heavy chain (IgH) alternative processing. Probably acts by regulating histone modifications accompanying transition from membrane-specific to secretory IgH mRNA expression. KEYWORDS: 3D-structure;Alternative splicing;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation;Ubl conjugation SUBCELLULAR LOCATION: Nucleus. " O00488,"PROTEIN NAMES: Zinc finger protein 593 (Zinc finger protein T86) PROTEIN FAMILY: ZNF593/BUD20 C2H2-type zinc-finger protein family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the ZNF593/BUD20 C2H2-type zinc-finger protein family. FUNCTION: Involved in pre-60S ribosomal particles maturation by promoting the nuclear export of the 60S ribosome. Negatively modulates the DNA binding activity of Oct-2 and therefore its transcriptional regulatory activity. KEYWORDS: 3D-structure;Cytoplasm;DNA-binding;Metal-binding;Nucleus;Reference proteome;Ribosome biogenesis;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus, nucleolus Cytoplasm Note=Shuttles between the nucleus and the cytoplasm. " O00499,"PROTEIN NAMES: Myc box-dependent-interacting protein 1 (Amphiphysin II) (Amphiphysin-like protein) (Box-dependent myc-interacting protein 1) (Bridging integrator 1) ORGANISM: Homo sapiens (Human) FUNCTION: Is a key player in the control of plasma membrane curvature, membrane shaping and membrane remodeling. Required in muscle cells for the formation of T-tubules, tubular invaginations of the plasma membrane that function in depolarization-contraction coupling. Is a negative regulator of endocytosis (By similarity). Is also involved in the regulation of intracellular vesicles sorting, modulation of BACE1 trafficking and the control of amyloid-beta production. In neuronal circuits, endocytosis regulation may influence the internalization of PHF-tau aggregates (By similarity). May be involved in the regulation of MYC activity and the control cell proliferation. Has actin bundling activity and stabilizes actin filaments against depolymerization in vitro. KEYWORDS: 3D-structure;Acetylation;Alternative splicing;Cell membrane;Coiled coil;Cytoplasm;Developmental protein;Differentiation;Disease variant;Endocytosis;Endosome;Host-virus interaction;Membrane;Nucleus;Phosphoprotein;Reference proteome;SH3 domain SUBCELLULAR LOCATION: [Isoform BIN1]: Nucleus Cytoplasm Endosome Cell membrane, sarcolemma, T-tubule.; SUBCELLULAR LOCATION: [Isoform IIA]: Cytoplasm " O00519,"PROTEIN NAMES: Fatty-acid amide hydrolase 1 (Anandamide amidohydrolase 1) (Fatty acid ester hydrolase) (Oleamide hydrolase 1) PROTEIN FAMILY: Amidase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the amidase family. FUNCTION: Catalyzes the hydrolysis of endogenous amidated lipids like the sleep-inducing lipid oleamide ((9Z)-octadecenamide), the endocannabinoid anandamide (N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-ethanolamine), as well as other fatty amides, to their corresponding fatty acids, thereby regulating the signaling functions of these molecules. Hydrolyzes polyunsaturated substrate anandamide preferentially as compared to monounsaturated substrates. It can also catalyze the hydrolysis of the endocannabinoid 2-arachidonoylglycerol (2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-glycerol). FAAH cooperates with PM20D1 in the hydrolysis of amino acid-conjugated fatty acids such as N-fatty acyl glycine and N-fatty acyl-L-serine, thereby acting as a physiological regulator of specific subsets of intracellular, but not of extracellular, N-fatty acyl amino acids (By similarity). KEYWORDS: Cytoplasm;Cytoskeleton;Direct protein sequencing;Hydrolase;Lipid degradation;Lipid metabolism;Membrane;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endomembrane system ; Single-pass membrane protein Cytoplasm, cytoskeleton Note=Seems to be attached to intracellular membranes and a portion of the cytoskeletal network. " O00541,"PROTEIN NAMES: Pescadillo homolog PROTEIN FAMILY: Pescadillo family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the pescadillo family. FUNCTION: Component of the PeBoW complex, which is required for maturation of 28S and 5.8S ribosomal RNAs and formation of the 60S ribosome. KEYWORDS: 3D-structure;Acetylation;Alternative splicing;Chromosome;Isopeptide bond;Nucleus;Reference proteome;Ribosome biogenesis;rRNA processing;Ubl conjugation SUBCELLULAR LOCATION: Nucleus, nucleolus. Nucleus, nucleoplasm. Chromosome. Note=Appears to localize to the periphery of metaphase chromosomes during mitosis and to the prenucleolar bodies that form in mitotic cells prior to the actual nucleoli. " O00591,"PROTEIN NAMES: Gamma-aminobutyric acid receptor subunit pi (GABA(A) receptor subunit pi) (GABAAR subunit pi) PROTEIN FAMILY: Ligand-gated ion channel family, Gamma-aminobutyric acid receptor subfamily, GABRP sub-subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRP sub-subfamily. FUNCTION: Pi subunit of the heteropentameric ligand-gated chloride channel gated by gamma-aminobutyric acid (GABA). GABA-gated chloride channels, also named GABA(A) receptors (GABAAR), consist of five subunits arranged around a central pore and contain GABA active binding site(s) located at the alpha and beta subunit interfaces (By similarity). When activated by GABA, GABAARs selectively allow the flow of chloride anions across the cell membrane down their electrochemical gradient. Pi-containing GABAARs are mostly located in peripheral tissues. In the uterus, pi subunits modulate uterus contraction by altering the sensitivity of GABAARs to pregnanolone. In the lungs, pi-containing GABAARs contribute to pulmonary fluid transport via luminal secretion of chloride (By similarity). KEYWORDS: Cell membrane;Chloride;Chloride channel;Disulfide bond;Glycoprotein;Ion channel;Ion transport;Membrane;Reference proteome;Signal;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein Apical cell membrane ; Multi-pass membrane protein Note=Located on the apical plasma membrane of alveolar epithelial type II cells. " O00628,"PROTEIN NAMES: Peroxisomal targeting signal 2 receptor (PTS2 receptor) (Peroxin-7) PROTEIN FAMILY: WD repeat peroxin-7 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the WD repeat peroxin-7 family. FUNCTION: Receptor required for the peroxisomal import of proteins containing a C-terminal PTS2-type peroxisomal targeting signal. Specifically binds to cargo proteins containing a PTS2 peroxisomal targeting signal in the cytosol. Cargo protein-binding triggers interaction with PEX5 and formation of a ternary complex composed of PEX5 and PEX7 along with PTS2-containing cargo proteins, which is tranlocated into peroxisomes by passing through the PEX13-PEX14 docking complex. KEYWORDS: Alternative splicing;Cataract;Cytoplasm;Deafness;Disease variant;Ichthyosis;Peroxisome;Peroxisome biogenesis disorder;Protein transport;Reference proteome;Repeat;Retinitis pigmentosa;Rhizomelic chondrodysplasia punctata;Transport;WD repeat SUBCELLULAR LOCATION: Cytoplasm, cytosol Peroxisome matrix Note=Translocated into the peroxisome matrix together with PTS2-containing cargo proteins and PEX5. " O00767,"PROTEIN NAMES: Stearoyl-CoA desaturase (hSCD1) (Acyl-CoA desaturase) (Delta(9)-desaturase) (Delta-9 desaturase) (Fatty acid desaturase) PROTEIN FAMILY: Fatty acid desaturase type 1 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the fatty acid desaturase type 1 family. FUNCTION: Stearoyl-CoA desaturase that utilizes O(2) and electrons from reduced cytochrome b5 to introduce the first double bond into saturated fatty acyl-CoA substrates. Catalyzes the insertion of a cis double bond at the delta-9 position into fatty acyl-CoA substrates including palmitoyl-CoA and stearoyl-CoA. Gives rise to a mixture of 16:1 and 18:1 unsaturated fatty acids. Plays an important role in lipid biosynthesis. Plays an important role in regulating the expression of genes that are involved in lipogenesis and in regulating mitochondrial fatty acid oxidation (By similarity). Plays an important role in body energy homeostasis (By similarity). Contributes to the biosynthesis of membrane phospholipids, cholesterol esters and triglycerides (By similarity). KEYWORDS: 3D-structure;Direct protein sequencing;Endoplasmic reticulum;Fatty acid biosynthesis;Fatty acid metabolism;Iron;Lipid biosynthesis;Lipid metabolism;Membrane;Metal-binding;Oxidoreductase;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein " O00910,"PROTEIN NAMES: Signal transducer and activator of transcription A (Dd-STATa) (STAT5 homolog A) PROTEIN FAMILY: Transcription factor STAT family ORGANISM: Dictyostelium discoideum (Social amoeba) SIMILARITY: Belongs to the transcription factor STAT family. FUNCTION: Transcription factor that binds to 5'-TTGAATTGA-3' elements in the promoter region of target genes. Functions as a repressor of the ecmB gene. Regulates the differentiation of prestalk cells during development. KEYWORDS: 3D-structure;Coiled coil;Cytoplasm;Direct protein sequencing;DNA-binding;Nucleus;Phosphoprotein;Reference proteome;Repressor;SH2 domain;Transcription;Transcription regulation SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Cytoplasmic in growing cells. Translocated into the nucleus in response to cAMP-induced tyrosine phosphorylation. Nuclear at the tight mound stage and in the upper, prestalk region of tipped aggregates and in cells at the tip of the slug. Subject to crm1-dependent nuclear export. " O01382,PROTEIN NAMES: Caspase (drICE) [Cleaved into: Caspase subunit p21; Caspase subunit p12] PROTEIN FAMILY: Peptidase C14A family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the peptidase C14A family. FUNCTION: Involved in the activation cascade of caspases responsible for apoptosis execution. Acts downstream of rpr. Cleaves baculovirus p35 and lamin DmO in vitro. KEYWORDS: 3D-structure;Apoptosis;Hydrolase;Protease;Reference proteome;Thiol protease;Zymogen O01704,"PROTEIN NAMES: Exostosin-1 homolog (Multiple exostoses homolog 1) (Related to mammalian RIB protein 1) PROTEIN FAMILY: Glycosyltransferase 47 family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the glycosyltransferase 47 family. FUNCTION: Required for the biosynthesis of heparan sulfate by positively regulating N-acetylglucosamine transferase II (GlcNAcT-II) and glucuronyl transferase II (GlcAT-II) activities of glycosyltransferase rib-2. Probably not directly involved in chondroitin sulfate biosynthesis but negatively regulates chondroitin sulfate levels. Maternally required for normal ventral epidermal enclosure and for embryo elongation during the early stages of embryonic development. In addition, involved in the elongation of the pharyngeal isthmus and in the organization of the actin cytoskeleton in the pharyngeal muscles during the later stages embryonic development. In adults, regulates egg-laying and the normal morphogenesis of the vulva. Also involved in the directed migration of hermaphrodite-specific neurons. KEYWORDS: Endoplasmic reticulum;Glycoprotein;Golgi apparatus;Reference proteome;Signal SUBCELLULAR LOCATION: Endoplasmic reticulum Golgi apparatus MISCELLANEOUS: Does not display any N-acetylglucosamine transferase II (GlcNAcT-II) and glucuronyl transferase II (GlcAT-II) activities when expressed alone in vitro." O01739,"PROTEIN NAMES: D-aspartate oxidase 3 (DASOX 3) (DASPO 3) (DDO-3) PROTEIN FAMILY: DAMOX/DASOX family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the DAMOX/DASOX family. FUNCTION: Selectively catalyzes the oxidative deamination of acidic amino acids. Plays a role in the egg-laying events and maturation processes of the reproductive organs. KEYWORDS: FAD;Flavoprotein;Glycoprotein;Oxidoreductase;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted Note=In hermaphrodites, protein secreted from proximal gonadal sheath cells may be transferred to the oocyte surface. In males, protein secreted from the seminal vesicle into the seminal fluid is transferred to the hermaphrodite uterus during mating. " O01798,"PROTEIN NAMES: Spermatocyte protein spe-8 (Defective spermatogenesis protein spe-8) PROTEIN FAMILY: Protein kinase superfamily, Tyr protein kinase family, Fes/fps subfamily ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. Fes/fps subfamily. FUNCTION: Probable non-receptor tyrosine-protein kinase which plays a role in spermatid activation (spermiogenesis) in hermaphrodites. KEYWORDS: ATP-binding;Cell membrane;Cytoplasm;Differentiation;Kinase;Membrane;Nucleotide-binding;Reference proteome;SH2 domain;Spermatogenesis;Transferase;Tyrosine-protein kinase SUBCELLULAR LOCATION: Cell membrane ; Peripheral membrane protein Cytoplasm Note=Localizes mainly in the cytoplasm of stage I spermatocytes and at the cell membrane of stage II spermatocytes and spermatids. " O01839,"PROTEIN NAMES: Vacuolar protein sorting-associated protein 51 homolog (Protein fat-free homolog) PROTEIN FAMILY: VPS51 family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the VPS51 family. FUNCTION: Acts as a component of the GARP complex that is involved in retrograde transport from early and late endosomes to the trans-Golgi network (TGN). The GARP complex facilitates tethering as well as SNARE complex assembly at the Golgi. Plays a role in the trafficking of cargo to dense-core vesicles, probably through association with the EARP-interacting protein eipr-1. Important for neuronal function. KEYWORDS: Coiled coil;Golgi apparatus;Reference proteome SUBCELLULAR LOCATION: Golgi apparatus, trans-Golgi network " O02213,"PROTEIN NAMES: Tyramine receptor Ser-2 PROTEIN FAMILY: G-protein coupled receptor 1 family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the G-protein coupled receptor 1 family. FUNCTION: G-protein coupled receptor for tyramine, a known neurotransmitter and neuromodulator and direct precursor of octopamine. The rank order of potency is tyramine > octopamine > dopamine > serotonin > epinephrine = norepinephrine. KEYWORDS: Alternative promoter usage;Alternative splicing;Cell membrane;Disulfide bond;G-protein coupled receptor;Glycoprotein;Membrane;Receptor;Reference proteome;Transducer;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. MISCELLANEOUS: [Isoform e]: Produced by alternative promoter usage.; MISCELLANEOUS: [Isoform a]: Produced by alternative splicing of isoform e.; MISCELLANEOUS: [Isoform b]: Produced by alternative splicing of isoform g.; MISCELLANEOUS: [Isoform c]: Produced by alternative splicing of isoform g.; MISCELLANEOUS: [Isoform d]: Produced by alternative promoter usage.; MISCELLANEOUS: [Isoform g]: Produced by alternative promoter usage." O02776,"PROTEIN NAMES: Poly(ADP-ribose) glycohydrolase PROTEIN FAMILY: Poly(ADP-ribose) glycohydrolase family ORGANISM: Bos taurus (Bovine) SIMILARITY: Belongs to the poly(ADP-ribose) glycohydrolase family. FUNCTION: Poly(ADP-ribose) glycohydrolase that degrades poly(ADP-ribose) by hydrolyzing the ribose-ribose bonds present in poly(ADP-ribose). PARG acts both as an endo- and exoglycosidase, releasing poly(ADP-ribose) of different length as well as ADP-ribose monomers. It is however unable to cleave the ester bond between the terminal ADP-ribose and ADP-ribosylated residues, leaving proteins that are mono-ADP-ribosylated. Poly(ADP-ribose) is synthesized after DNA damage is only present transiently and is rapidly degraded by PARG. Required to prevent detrimental accumulation of poly(ADP-ribose) upon prolonged replicative stress, while it is not required for recovery from transient replicative stress. Responsible for the prevalence of mono-ADP-ribosylated proteins in cells, thanks to its ability to degrade poly(ADP-ribose) without cleaving the terminal protein-ribose bond. Required for retinoid acid-dependent gene transactivation, probably by removing poly(ADP-ribose) from histone demethylase KDM4D, allowing chromatin derepression at RAR-dependent gene promoters. Involved in the synthesis of ATP in the nucleus, together with PARP1, NMNAT1 and NUDT5. Nuclear ATP generation is required for extensive chromatin remodeling events that are energy-consuming (By similarity). KEYWORDS: Acetylation;Direct protein sequencing;DNA damage;Hydrolase;Nucleus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Nucleus Note=Colocalizes with PCNA at replication foci. Relocalizes to the cytoplasm in response to DNA damage (By similarity). " O04130,"PROTEIN NAMES: D-3-phosphoglycerate dehydrogenase 2, chloroplastic (PGDH) PROTEIN FAMILY: D-isomer specific 2-hydroxyacid dehydrogenase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. FUNCTION: Involved in the plastidial phosphorylated pathway of serine biosynthesis (PPSB). PATHWAY: Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 1/3. KEYWORDS: Alternative splicing;Amino-acid biosynthesis;Chloroplast;NAD;Oxidoreductase;Phosphoprotein;Plastid;Reference proteome;Serine biosynthesis;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast " O04151,"PROTEIN NAMES: Calreticulin-1 PROTEIN FAMILY: Calreticulin family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the calreticulin family. FUNCTION: Molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin may interact transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER (By similarity). KEYWORDS: Alternative splicing;Calcium;Chaperone;Disulfide bond;Endoplasmic reticulum;Glycoprotein;Lectin;Metal-binding;Phosphoprotein;Reference proteome;Repeat;Signal;Zinc SUBCELLULAR LOCATION: Endoplasmic reticulum lumen " O04202,"PROTEIN NAMES: Eukaryotic translation initiation factor 3 subunit F (eIF3f) (eIF-3-epsilon) (eIF3 p32 subunit) PROTEIN FAMILY: EIF-3 subunit F family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the eIF-3 subunit F family. FUNCTION: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation (Potential). Involved in cell growth and differentiation, especially during embryogenesis and male gametophyte germination. Regulates sensitivity to sugars (e.g. sucrose). KEYWORDS: Acetylation;Cytoplasm;Initiation factor;Protein biosynthesis;Reference proteome SUBCELLULAR LOCATION: Cytoplasm " O04523,"PROTEIN NAMES: Xyloglucan O-acetyltransferase 1 (Protein ALTERED XYLOGLUCAN 4) (Protein TRICHOME BIREFRINGENCE-LIKE 27) PROTEIN FAMILY: PC-esterase family, TBL subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the PC-esterase family. TBL subfamily. FUNCTION: Xyloglucan acetyltransferase that catalyzes the acetylation of fucosylated Gal residues on xyloglucan side chains. Predominantly catalyze 6-O-monoacetylation of Gal residues in the Fuc-Gal-Xyl trisaccharide side chains of xyloglucan oligomers. Involved in xyloglucan specific O-acetylation in roots and rosette leaves. KEYWORDS: Disulfide bond;Glycoprotein;Golgi apparatus;Membrane;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus membrane ; Single-pass type II membrane protein MISCELLANEOUS: Contains 2 motifs that are conserved in esterases, but it is unlikely that this protein belongs to the catalytically active pectin esterases.; MISCELLANEOUS: A naturally occurring ecotype (cv. Ty-0) lacks AXY4-mediated xyloglucan O-acetylation due to 2 amino acid changes, Asp367Glu and Gly368Lys." O04795,"PROTEIN NAMES: Anionic peroxidase (SwPA1) PROTEIN FAMILY: Peroxidase family, Classical plant (class III) peroxidase subfamily ORGANISM: Ipomoea batatas (Sweet potato) (Convolvulus batatas) SIMILARITY: Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily. FUNCTION: Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.; FUNCTION: May contribute to protection against cold-induced oxidative stress. KEYWORDS: Calcium;Direct protein sequencing;Disulfide bond;Glycoprotein;Heme;Hydrogen peroxide;Iron;Metal-binding;Oxidoreductase;Peroxidase;Secreted;Signal SUBCELLULAR LOCATION: Secreted " O05131,PROTEIN NAMES: Penicillin-binding protein 1A (PBP-1a) (PBP1a) [Includes: Penicillin-insensitive transglycosylase (Peptidoglycan TGase); Penicillin-sensitive transpeptidase (DD-transpeptidase)] PROTEIN FAMILY: Glycosyltransferase 51 family; Transpeptidase family ORGANISM: Neisseria gonorrhoeae SIMILARITY: In the N-terminal section; belongs to the glycosyltransferase 51 family.; SIMILARITY: In the C-terminal section; belongs to the transpeptidase family. FUNCTION: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal domain (cross-linking of the peptide subunits). Essential for cell wall synthesis. PATHWAY: Cell wall biogenesis; peptidoglycan biosynthesis. KEYWORDS: Antibiotic resistance;Carboxypeptidase;Cell inner membrane;Cell membrane;Cell shape;Cell wall biogenesis/degradation;Direct protein sequencing;Glycosyltransferase;Hydrolase;Membrane;Multifunctional enzyme;Peptidoglycan synthesis;Protease;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell inner membrane ; Single-pass type II membrane protein O05307,PROTEIN NAMES: Medium/long-chain-fatty-acid--CoA ligase FadD6 (FACL6) PROTEIN FAMILY: ATP-dependent AMP-binding enzyme family ORGANISM: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) SIMILARITY: Belongs to the ATP-dependent AMP-binding enzyme family. FUNCTION: Catalyzes the activation of medium/long-chain fatty acids as acyl-coenzyme A (acyl-CoA). May play a role in the uptake of fatty acids by trapping them metabolically as CoA esters. May also play an important role in the channeling of fatty acids into triacylglycerol (TAG) for use by Mycobacterium during its dormancy. KEYWORDS: ATP-binding;Fatty acid metabolism;Ligase;Lipid metabolism;Nucleotide-binding;Reference proteome MISCELLANEOUS: Stimulates fatty acid uptake in E.coli cells. O05542,"PROTEIN NAMES: Alcohol dehydrogenase (quinone), dehydrogenase subunit (ADH) (Alcohol dehydrogenase (quinone), acceptor subunit) (Alcohol dehydrogenase (quinone), subunit I) (Ethanol:Q2 reductase) (G3-ADH subunit I) (Quinohemoprotein alcohol dehydrogenase) (Quinohemoprotein-cytochrome c complex) (Ubiquinol oxidase) PROTEIN FAMILY: Bacterial PQQ dehydrogenase family ORGANISM: Gluconobacter oxydans (strain 621H) (Gluconobacter suboxydans) SIMILARITY: Belongs to the bacterial PQQ dehydrogenase family. FUNCTION: Dehydrogenase component of the alcohol dehydrogenase multicomponent enzyme system which is involved in the production of acetic acid and in the ethanol oxidase respiratory chain. Quinohemoprotein alcohol dehydrogenase (ADH) catalyzes the oxidation of ethanol to acetaldehyde by transferring electrons to the ubiquinone embedded in the membrane phospholipids. The electrons transfer from ethanol to membranous ubiquinone occurs from pyrroloquinoline quinone (PQQ) to one heme c in subunit I (AdhA), and finally to two heme c in subunit II (AdhB). Besides ubiquinone reduction, ADH also has a ubiquinol (QH2) oxidation reaction which mediates electron transfer from ubiquinol to the non-energy generating bypass oxidase system. The electrons transfer occurs from ubiquinol (QH2) to the additional heme c within subunit II (AdhB). Also able to use quinone analogs such as 2,3-dimethoxy-5-methyl-6-n-decyl-1,4-benzoquinone (DB) and 2,3-dimethoxy-5-methyl-6-n-pentyl-1,4-benzoquinone (PB). KEYWORDS: 3D-structure;Calcium;Cell membrane;Direct protein sequencing;Disulfide bond;Electron transport;Heme;Iron;Membrane;Metal-binding;Oxidoreductase;PQQ;Pyrrolidone carboxylic acid;Reference proteome;Respiratory chain;Signal;Transport SUBCELLULAR LOCATION: Cell membrane ; Peripheral membrane protein ; Periplasmic side MISCELLANEOUS: Inactive ADH is produced under conditions of low pH and high aeration, where the bypass oxidase activity is highly elevated. In spite of having 10 times less enzyme activity than active ADH, inactive ADH is not distinguished from active ADH with respect to their subunit compositions, molecular sizes and prosthetic groups. It seems that in inactive ADH, an improper interaction between subunit II and subunit I/III complex impairs efficient intersubunit electron transport in the ADH complex." O06582,PROTEIN NAMES: 2-methylcitrate dehydratase (2-MC dehydratase) (Aconitate hydratase) (ACN) (Aconitase) PROTEIN FAMILY: PrpD family ORGANISM: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) SIMILARITY: Belongs to the PrpD family. FUNCTION: Involved in the catabolism of short chain fatty acids (SCFA) via the tricarboxylic acid (TCA)(acetyl degradation route) and via the 2-methylcitrate cycle I (propionate degradation route). Catalyzes the dehydration of 2-methylcitrate (2-MC) to yield the cis isomer of 2-methyl-aconitate. Could also catalyze the dehydration of citrate and the hydration of cis-aconitate (By similarity). PATHWAY: Organic acid metabolism; propanoate degradation.; PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 1/2. KEYWORDS: Cholesterol metabolism;Lipid metabolism;Lyase;Reference proteome;Steroid metabolism;Sterol metabolism;Tricarboxylic acid cycle MISCELLANEOUS: The vitamin B12 restores growth of the prpDC mutant on propionate as the sole carbon source. It suggests the capacity of the MCM-dependent methylmalonyl pathway to support the metabolism of propionate independently of the methylcitrate cycle. O06769,"PROTEIN NAMES: Neutral ceramidase (N-CDase) (NCDase) (Acylsphingosine deacylase) (N-acylsphingosine amidohydrolase) PROTEIN FAMILY: Neutral ceramidase family ORGANISM: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) SIMILARITY: Belongs to the neutral ceramidase family. FUNCTION: Catalyzes the cleavage of the N-acyl linkage of the ceramides (Cers) to yield sphingosine (Sph) and free fatty acid. Also catalyzes the synthesis of Cers from Sph and fatty acid. Cers containning C6-C24 fatty acids are well hydrolyzed, and Cers with mono unsaturated fatty acids are much more hydrolyzed than those with saturated fatty acids. KEYWORDS: Hydrolase;Lipid metabolism;Magnesium;Metal-binding;Reference proteome;Zinc " O07834,PROTEIN NAMES: Dipeptidyl aminopeptidase BI (DAP BI) PROTEIN FAMILY: Peptidase S9A family ORGANISM: Pseudoxanthomonas mexicana SIMILARITY: Belongs to the peptidase S9A family. FUNCTION: Sequentially removes dipeptide units (NH3-P2-P1-) from the amino termini of peptides and proteins. Is able to catalyze the removal of Asp-Arg from the amino termini of angiotensins I and II. Has slight endopeptidase activity on N-terminally blocked peptide derivatives which contain arginine residues at the P1 position. Does not hydrolyze Ala-Ala-Ala and Ala-Ala-Ala-Ala substrates or insulin beta chain. KEYWORDS: Aminopeptidase;Direct protein sequencing;Hydrolase;Protease;Serine protease;Signal O08498,"PROTEIN NAMES: Metallo-beta-lactamase type 2 (B2 metallo-beta-lactamase) (Beta-lactamase type II) (Carbapenem-hydrolyzing beta-lactamase BlaB-1) (CHbetaL-1) (Class B carbapenemase BlaB-1) (Metallo-beta-lactamase type II) PROTEIN FAMILY: Metallo-beta-lactamase superfamily, Class-B beta-lactamase family ORGANISM: Elizabethkingia meningoseptica (Chryseobacterium meningosepticum) SIMILARITY: Belongs to the metallo-beta-lactamase superfamily. Class-B beta-lactamase family. FUNCTION: Confers resistance to the different beta-lactams antibiotics (penicillin, cephalosporin and carbapenem) via the hydrolysis of the beta-lactam ring. KEYWORDS: 3D-structure;Antibiotic resistance;Direct protein sequencing;Hydrolase;Metal-binding;Periplasm;Signal;Zinc SUBCELLULAR LOCATION: Periplasm " O08524,"PROTEIN NAMES: Beta-tectorin ORGANISM: Mus musculus (Mouse) FUNCTION: One of the major non-collagenous components of the tectorial membrane (By similarity). The tectorial membrane is an extracellular matrix of the inner ear that covers the neuroepithelium of the cochlea and contacts the stereocilia bundles of specialized sensory hair cells. Sound induces movement of these hair cells relative to the tectorial membrane, deflects the stereocilia and leads to fluctuations in hair-cell membrane potential, transducing sound into electrical signals. KEYWORDS: Cell membrane;Disulfide bond;Extracellular matrix;Glycoprotein;GPI-anchor;Lipoprotein;Membrane;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Cell membrane ; Lipid-anchor, GPI-anchor ; Extracellular side Secreted, extracellular space, extracellular matrix. Note=Found in the non-collagenous matrix of the tectorial membrane. " O08760,"PROTEIN NAMES: N-glycosylase/DNA lyase [Includes: 8-oxoguanine DNA glycosylase ; DNA-(apurinic or apyrimidinic site) lyase (AP lyase) ] PROTEIN FAMILY: Type-1 OGG1 family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the type-1 OGG1 family. FUNCTION: DNA repair enzyme that incises DNA at 8-oxoG residues. Excises 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine (FAPY) from damaged DNA. Has a beta-lyase activity that nicks DNA 3' to the lesion. KEYWORDS: 3D-structure;DNA damage;DNA repair;Glycosidase;Hydrolase;Lyase;Multifunctional enzyme;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus, nucleoplasm Nucleus speckle Nucleus matrix Note=Together with APEX1 is recruited to nuclear speckles in UVA-irradiated cells. " O08876,"PROTEIN NAMES: Krueppel-like factor 10 (Transforming growth factor-beta-inducible early growth response protein 1) (TGFB-inducible early growth response protein 1) (TIEG-1) (Zinc finger transcription factor homolog CPG20) PROTEIN FAMILY: Sp1 C2H2-type zinc-finger protein family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the Sp1 C2H2-type zinc-finger protein family. FUNCTION: Transcriptional repressor which binds to the consensus sequence 5'-GGTGTG-3'. Regulates the circadian expression of genes involved in lipogenesis, gluconeogenesis, and glycolysis in the liver. Represses the expression of PCK2, a rate-limiting step enzyme of gluconeogenesis. May play a role in the cell cycle regulation (By similarity). Plays a role in the regulation of the circadian clock; binds to the GC box sequence in the promoter of the core clock component ARTNL/BMAL1 and represses its transcriptional activity. KEYWORDS: Biological rhythms;DNA-binding;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Repeat;Repressor;Transcription;Transcription regulation;Ubl conjugation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus " O09037,"PROTEIN NAMES: Regenerating islet-derived protein 3-alpha (REG-3-alpha) (Islet of Langerhans regenerating protein 3) (Lithostathine 3) (Pancreatitis-associated protein 2) (Regenerating islet-derived protein III-alpha) (Reg III-alpha) [Cleaved into: Regenerating islet-derived protein 3-alpha 16.5 kDa form; Regenerating islet-derived protein 3-alpha 15 kDa form] ORGANISM: Mus musculus (Mouse) FUNCTION: Bactericidal C-type lectin. The lack of the EPN motif may explain its inability to bind peptidoglycan.; FUNCTION: Acts as a hormone in response to different stimuli like anti-inflammatory signals, such as IL17A, or gut microbiome. Secreted by different cell types to activate its receptor EXTL3 and induce cell specific signaling pathways. Induced by IL17A in keratinocytes, regulates keratinocyte proliferation and differentiation after skin injury via activation of EXTL3-PI3K-AKT signaling pathway (By similarity). In parallel, inhibits skin inflammation through the inhibition of inflammatory cytokines such as IL6 and TNF. In pancreas, is able to permealize beta-cells membrane and stimulate their proliferation. KEYWORDS: Acute phase;Antimicrobial;Disulfide bond;Inflammatory response;Lectin;Metal-binding;Reference proteome;Secreted;Signal;Zinc SUBCELLULAR LOCATION: Secreted Note=Found in the apical region of pancreatic acinar cells. " O09100,PROTEIN NAMES: Endothelial transcription factor GATA-2 (GATA-binding protein 2) ORGANISM: Mus musculus (Mouse) FUNCTION: Transcriptional activator which regulates endothelin-1 gene expression in endothelial cells. Binds to the consensus sequence 5'-AGATAG-3'. KEYWORDS: Activator;DNA-binding;Isopeptide bond;Metal-binding;Methylation;Nucleus;Phagocytosis;Phosphoprotein;Reference proteome;Repeat;Transcription;Transcription regulation;Ubl conjugation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus. O09172,"PROTEIN NAMES: Glutamate--cysteine ligase regulatory subunit (GCS light chain) (Gamma-ECS regulatory subunit) (Gamma-glutamylcysteine synthetase regulatory subunit) (Glutamate--cysteine ligase modifier subunit) PROTEIN FAMILY: Aldo/keto reductase family, Glutamate--cysteine ligase light chain subfamily ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the aldo/keto reductase family. Glutamate--cysteine ligase light chain subfamily. PATHWAY: Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2. KEYWORDS: Acetylation;Glutathione biosynthesis;Phosphoprotein;Reference proteome " O13282,PROTEIN NAMES: Transcription initiation factor TFIID subunit 5 (Transcription initiation factor TFIID 72 kDa subunit) (TAFII-72) PROTEIN FAMILY: WD repeat TAF5 family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the WD repeat TAF5 family. FUNCTION: TAFs are components of the transcription factor IID (TFIID) complex that are essential for mediating regulation of RNA polymerase transcription. Regulates the genes involved in ubiquitin-dependent proteolysis during the progression of M-phase of mitosis. KEYWORDS: Nucleus;Phosphoprotein;Reference proteome;Repeat;Transcription;Transcription regulation;WD repeat SUBCELLULAR LOCATION: Nucleus O13339,"PROTEIN NAMES: Telomerase reverse transcriptase (Telomerase catalytic subunit) PROTEIN FAMILY: Reverse transcriptase family, Telomerase subfamily ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the reverse transcriptase family. Telomerase subfamily. FUNCTION: Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. It elongates telomeres. It is a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme. KEYWORDS: Alternative splicing;Chromosome;DNA-binding;Magnesium;Metal-binding;Nucleotidyltransferase;Nucleus;Reference proteome;RNA-directed DNA polymerase;Telomere;Transferase SUBCELLULAR LOCATION: Nucleus. Chromosome, telomere. MISCELLANEOUS: Deletion causes telomere shortening and senescence." O13601,"PROTEIN NAMES: DnaJ-related protein rsp1 ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) FUNCTION: Has a role in the proper organization of the interphase microtubule cytoskeleton. Required for equatorial microtubule organizing center (eMTOC) disassembly into satellites, contributing to the dynamic redistribution of MTOC components for organization of interphase microtubules. KEYWORDS: Chaperone;Cytoplasm;Cytoskeleton;Microtubule;Nucleus;Reference proteome SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton Nucleus Note=Associates with the microtubule bundles. " O13648,"PROTEIN NAMES: Ribosomal protein arginine N-methyltransferase rmt3 PROTEIN FAMILY: Class I-like SAM-binding methyltransferase superfamily, Protein arginine N-methyltransferase family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N-methyltransferase family. FUNCTION: Methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in ribosomal protein rps2. KEYWORDS: Cytoplasm;Metal-binding;Methyltransferase;Reference proteome;S-adenosyl-L-methionine;Transferase;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cytoplasm " O13688,PROTEIN NAMES: Non-structural maintenance of chromosome element 6 (Non-SMC element 6) (Core protein 1) ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) FUNCTION: Acts in a DNA repair pathway for removal of UV-induced DNA damage that is distinct from classical nucleotide excision repair and in repair of ionizing radiation damage. Functions in homologous recombination repair of DNA double strand breaks and in recovery of stalled replication forks. May prevent formation of excessive Holliday junctions or assist in their resolution. KEYWORDS: Chromosome;Direct protein sequencing;DNA damage;DNA recombination;DNA repair;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus Chromosome O13692,"PROTEIN NAMES: 1,3-beta-glucanosyltransferase gas5 PROTEIN FAMILY: Glycosyl hydrolase 72 family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the glycosyl hydrolase 72 family. FUNCTION: Splits internally a 1,3-beta-glucan molecule and transfers the newly generated reducing end (the donor) to the non-reducing end of another 1,3-beta-glucan molecule (the acceptor) forming a 1,3-beta linkage, resulting in the elongation of 1,3-beta-glucan chains in the cell wall. KEYWORDS: Cell wall;Cell wall biogenesis/degradation;Disulfide bond;Glycoprotein;GPI-anchor;Lipoprotein;Membrane;Reference proteome;Secreted;Signal;Transferase SUBCELLULAR LOCATION: Secreted, cell wall Membrane ; Lipid-anchor, GPI-anchor Note=Covalently-linked GPI-modified cell wall protein (GPI-CWP). " O13716,"PROTEIN NAMES: Glucan endo-1,3-alpha-glucosidase agn1 (Endo-1,3-alpha-glucanase agn1) PROTEIN FAMILY: Glycosyl hydrolase 71 family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the glycosyl hydrolase 71 family. FUNCTION: Has a role in cell separation where it is required for the degradation of the cell wall material surrounding the septum (the septum edging) which must be hydrolyzed before full separation of the daughter cells can occur. Hydrolyzes 1,3-alpha-glucan predominantly into pentasaccharides. KEYWORDS: Cell cycle;Cell division;Cell wall;Cell wall biogenesis/degradation;Glycosidase;Hydrolase;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted Secreted, cell wall Note=Associates with the cell wall. " O13788,"PROTEIN NAMES: SWI/SNF and RSC complexes subunit ssr1 PROTEIN FAMILY: SMARCC family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the SMARCC family. FUNCTION: Component of the chromatin structure remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. Controls particularly membrane and organelle development genes. Part of the SWI/SNF complex, an ATP-dependent chromatin remodeling complex, required for the positive and negative regulation of gene expression of a large number of genes. It changes chromatin structure by altering DNA-histone contacts within a nucleosome, leading eventually to a change in nucleosome position, thus facilitating or repressing binding of gene-specific transcription factors. KEYWORDS: Chromatin regulator;Cytoplasm;Metal-binding;Nucleus;Reference proteome;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cytoplasm Nucleus " O13790,"PROTEIN NAMES: Cullin-1 (Cul-1) (Cell division control 53 homolog) PROTEIN FAMILY: Cullin family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the cullin family. FUNCTION: Core component of multiple cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. The functional specificity of the SCF complex depends on the F-box protein as substrate recognition component. SCF(pop1-pop2) is required for the maintenance of ploidy and directs ubiquitination of cig2. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Cell cycle;Cell division;Cytoplasm;Isopeptide bond;Reference proteome;Ubl conjugation SUBCELLULAR LOCATION: Cytoplasm " O13795,"PROTEIN NAMES: La-related protein 7 homolog PROTEIN FAMILY: LARP7 family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the LARP7 family. FUNCTION: RNA-binding protein required for assembly of the holoenzyme telomerase ribonucleoprotein (RNP) complex. Specifically binds telomerase RNA ter1 and promotes assembly of ter1 with catalytic subunit trt1. Telomerase is a ribonucleoprotein enzyme essential that copies new telomeric repeats onto chromosome ends and functions to maintain cell division. KEYWORDS: 3D-structure;Chromosome;Cytoplasm;Nucleus;Reference proteome;RNA-binding;Telomere SUBCELLULAR LOCATION: Chromosome, telomere Nucleus Cytoplasm " O13816,"PROTEIN NAMES: Cohesin subunit psc3 (SCC3 homolog) PROTEIN FAMILY: SCC3 family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the SCC3 family. FUNCTION: Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the rad21 subunit of the cohesin complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. KEYWORDS: Cell cycle;Cell division;Centromere;Chromosome;Chromosome partition;Coiled coil;Mitosis;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus Chromosome, centromere Note=Associates with chromatin. Cohesin complex mainly associates with broad centromere region. Also associates with mating-type heterochromatic region. " O13820,"PROTEIN NAMES: C-22 sterol desaturase ERG5 (Cytochrome P450 61) (Ergosterol biosynthetic protein 5) PROTEIN FAMILY: Cytochrome P450 family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the cytochrome P450 family. FUNCTION: C-22 sterol desaturase; part of the third module of ergosterol biosynthesis pathway that includes by the late steps of the pathway. Erg5 converts 5-dehydroepisterol into ergosta-5,7,22,24(28)-tetraen-3beta-ol by forming the C-22(23) double bond in the sterol side chain (By similarity). The third module or late pathway involves the ergosterol synthesis itself through consecutive reactions that mainly occur in the endoplasmic reticulum (ER) membrane. Firstly, the squalene synthase erg9 catalyzes the condensation of 2 farnesyl pyrophosphate moieties to form squalene, which is the precursor of all steroids. Secondly, squalene is converted into lanosterol by the consecutive action of the squalene epoxidase erg1 and the lanosterol synthase erg7. The lanosterol 14-alpha-demethylase erg11/cyp1 catalyzes C14-demethylation of lanosterol to produce 4,4'-dimethyl cholesta-8,14,24-triene-3-beta-ol. In the next steps, a complex process involving various demethylation, reduction and desaturation reactions catalyzed by the C-14 reductase erg24 and the C-4 demethylation complex erg25-erg26-erg27 leads to the production of zymosterol. Erg28 likely functions in the C-4 demethylation complex reaction by tethering erg26 and Erg27 to the endoplasmic reticulum or to facilitate interaction between these proteins. Then, the sterol 24-C-methyltransferase erg6 catalyzes the methyl transfer from S-adenosyl-methionine to the C-24 of zymosterol to form fecosterol. The C-8 sterol isomerase erg2 catalyzes the reaction which results in unsaturation at C-7 in the B ring of sterols and thus converts fecosterol to episterol. The sterol-C5-desaturases erg31 and erg32 then catalyze the introduction of a C-5 double bond in the B ring to produce 5-dehydroepisterol. The C-22 sterol desaturase erg5 further converts 5-dehydroepisterol into ergosta-5,7,22,24(28)-tetraen-3beta-ol by forming the C-22(23) double bond in the sterol side chain. Finally, ergosta-5,7,22,24(28)-tetraen-3beta-ol is substrate of the C-24(28) sterol reductase erg4 to produce ergosterol (Probable). In the genus Schizosaccharomyces, a second route exists between lanosterol and fecosterol, via the methylation of lanosterol to eburicol by erg6, followed by C14-demethylation by erg11/cyp1 and C4-demethylation by the demethylation complex erg25-erg26-erg27 (Probable). PATHWAY: Steroid metabolism; ergosterol biosynthesis. KEYWORDS: Heme;Iron;Lipid biosynthesis;Lipid metabolism;Metal-binding;Oxidoreductase;Reference proteome;Steroid biosynthesis;Steroid metabolism;Sterol biosynthesis;Sterol metabolism;Stress response MISCELLANEOUS: In Aspergillus, the biosynthesis pathway of the sterol precursors leading to the prevalent sterol ergosterol differs from yeast. The ringsystem of lanosterol in S.cerevisiae is firstly demethylised in three enzymatic steps leading to the intermediate zymosterol and secondly a methyl group is added to zymosterol by the sterol 24-C-methyltransferase to form fecosterol. In Aspergillus, lanosterol is firstly transmethylated by the sterol 24-C-methyltransferase leading to the intermediate eburicol and secondly demethylated in three steps to form fecosterol. In the genus Schizosaccharomyces, 2 routes exist from lanosterol to erposterol: the classical one via zymosterol and the second one via the formation of eburicol followed by demethylation." O13836,"PROTEIN NAMES: Decapping nuclease din1 (Dhp1-interacting protein 1) (NAD-capped RNA hydrolase Rai1) (DeNADding enzyme Rai1) (spRai1) PROTEIN FAMILY: DXO/Dom3Z family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the DXO/Dom3Z family. FUNCTION: Decapping enzyme for NAD-capped RNAs: specifically hydrolyzes the nicotinamide adenine dinucleotide (NAD) cap from a subset of RNAs by removing the entire NAD moiety from the 5'-end of an NAD-capped RNA. The NAD-cap is present at the 5'-end of some RNAs and snoRNAs (By similarity). In contrast to the canonical 5'-end N7 methylguanosine (m7G) cap, the NAD cap promotes mRNA decay (By similarity). Also acts as a non-canonical decapping enzyme that removes the entire cap structure of m7G capped or incompletely capped RNAs and mediates their subsequent degradation. Specifically degrades pre-mRNAs with a defective m7G cap and is part of a pre-mRNA capping quality control. Has decapping activity toward incomplete 5'-end m7G cap mRNAs such as unmethylated 5'-end-capped RNA (cap0), while it has no activity toward 2'-O-ribose methylated m7G cap (cap1). Also possesses RNA 5'-pyrophosphohydrolase activity by hydrolyzing the 5'-end triphosphate to release pyrophosphates. Stimulates exoribonuclease activity of dhp1, allowing it to degrade RNAs with stable secondary structure more effectively. KEYWORDS: 3D-structure;Hydrolase;Metal-binding;mRNA processing;Nuclease;Nucleotide-binding;Nucleus;Phosphoprotein;Reference proteome;RNA-binding SUBCELLULAR LOCATION: Nucleus " O13842,PROTEIN NAMES: Putative polysaccharide deacetylase (Chitin deacetylase 1) PROTEIN FAMILY: Polysaccharide deacetylase family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the polysaccharide deacetylase family. FUNCTION: May deacetylate chitin (Probable). Required for spore formation. KEYWORDS: Cell wall biogenesis/degradation;Chitin-binding;Hydrolase;Metal-binding;Reference proteome;Sporulation SUBCELLULAR LOCATION: Prospore O13853,"PROTEIN NAMES: Phosphatidylinositol 4-phosphate 5-kinase its3 (1-phosphatidylinositol 4-phosphate kinase) (Diphosphoinositide kinase) (PIP5K) (PtdIns(4)P-5-kinase) ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) FUNCTION: Involved, together with the calcineurin ppb1, in cytokinesis. KEYWORDS: ATP-binding;Cell cycle;Cell division;Kinase;Membrane;Nucleotide-binding;Nucleus;Phosphoprotein;Reference proteome;Transferase SUBCELLULAR LOCATION: Nucleus. Membrane; Peripheral membrane protein. Note=The active form of the enzyme is membrane-associated and concentrates at the septum in dividing cells. " O13881,"PROTEIN NAMES: Cryptic loci regulator 2 ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) FUNCTION: Required for deacetylation in the mating-type region and the centromere. Acts upstream of the histone deacetylases to promote transcriptional silencing. Required for proper positioning of nucleosomes at heterochromatic loci and for transcriptional gene silencing (TGS) function of the Snf2/Hdac-containing repressor complex (SHREC). KEYWORDS: 3D-structure;Centromere;Chromatin regulator;Chromosome;Nucleus;Reference proteome;Repressor;Telomere;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus Chromosome, centromere Chromosome, telomere Note=Associates with major heterochromatin, centromeres, sub-telomeres, rDNA and the mat locus. " O13899,PROTEIN NAMES: Cardiolipin synthase (CMP-forming) / mitochondrial hydrolase fusion protein [Cleaved into: Mitochondrial hydrolase ; Cardiolipin synthase (CMP-forming) (CLS) ] PROTEIN FAMILY: HAD-like hydrolase superfamily; CDP-alcohol phosphatidyltransferase class-I family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: In the N-terminal section; belongs to the HAD-like hydrolase superfamily.; SIMILARITY: In the C-terminal section; belongs to the CDP-alcohol phosphatidyltransferase class-I family. FUNCTION: [Cardiolipin synthase (CMP-forming)]: Catalyzes the synthesis of cardiolipin (CL) (diphosphatidylglycerol) by specifically transferring a phosphatidyl group from CDP-diacylglycerol to phosphatidylglycerol (PG). CL is a key phospholipid in mitochondrial membranes and plays important roles in maintaining the functional integrity and dynamics of mitochondria under both optimal and stress conditions.; FUNCTION: [Mitochondrial hydrolase]: Activity is dispensable for viability. KEYWORDS: Alternative splicing;Hydrolase;Lipid biosynthesis;Lipid metabolism;Membrane;Mitochondrion;Mitochondrion inner membrane;Phospholipid biosynthesis;Phospholipid metabolism;Reference proteome;Transferase;Transit peptide;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Mitochondrion.; SUBCELLULAR LOCATION: [Cardiolipin synthase (CMP-forming)]: Mitochondrion inner membrane ; Multi-pass membrane protein.; SUBCELLULAR LOCATION: [Mitochondrial hydrolase]: Mitochondrion O13902,PROTEIN NAMES: Dihydroxyacetone kinase 1 (DHA kinase 1) (Glycerone kinase 1) (Triokinase 1) (Triose kinase 1) PROTEIN FAMILY: Dihydroxyacetone kinase (DAK) family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the dihydroxyacetone kinase (DAK) family. FUNCTION: Catalyzes both the phosphorylation of dihydroxyacetone and of glyceraldehyde. PATHWAY: Polyol metabolism; glycerol fermentation; glycerone phosphate from glycerol (oxidative route): step 2/2. KEYWORDS: ATP-binding;Glycerol metabolism;Kinase;Nucleotide-binding;Reference proteome;Transferase O13918,"PROTEIN NAMES: Zinc homeostasis factor 1 PROTEIN FAMILY: Cation diffusion facilitator (CDF) transporter family, SLC30A subfamily ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. SLC30A subfamily. FUNCTION: Involved in zinc homeostasis, where it plays a role in its accumulation in the endoplasmic reticulum/nucleus. Also has a role in the sequestration of cadmium into the endoplasmic reticulum. KEYWORDS: Endoplasmic reticulum;Ion transport;Membrane;Nucleus;Reference proteome;Transmembrane;Transmembrane helix;Transport;Zinc;Zinc transport SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein Nucleus membrane ; Multi-pass membrane protein " O13924,"PROTEIN NAMES: Serine/threonine-protein kinase haspin homolog hrk1 PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family, Haspin subfamily ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Haspin subfamily. FUNCTION: Serine/threonine haspin-like protein kinase involved in cell cycle regulation. Acts in chromosomal passenger complex (CPC) targeting to centromeres by phosphorylating histone H3 at 'Thr3' (H3T3ph). KEYWORDS: ATP-binding;Cell cycle;Chromosome;Cytoplasm;Kinase;Nucleotide-binding;Reference proteome;Serine/threonine-protein kinase;Transferase SUBCELLULAR LOCATION: Cytoplasm. Chromosome. " O13936,"PROTEIN NAMES: Transcription elongation factor spt5 (Chromatin elongation factor spt5) PROTEIN FAMILY: SPT5 family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the SPT5 family. FUNCTION: The spt4-spt5 complex mediates both activation and inhibition of transcription elongation, and plays a role in pre-mRNA processing. This complex seems to be important for the stability of the RNA polymerase II elongation machinery on the chromatin template but not for the inherent ability of this machinery to translocate down the gene (By similarity). KEYWORDS: 3D-structure;mRNA processing;Nucleus;Phosphoprotein;Reference proteome;Repeat;Transcription SUBCELLULAR LOCATION: Nucleus " O13953,"PROTEIN NAMES: Chromatin modification-related protein eaf3 (Altered polarity protein 13) (ESA1-associated factor 3) PROTEIN FAMILY: MRG family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the MRG family. FUNCTION: Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of selected genes principally by acetylation of nucleosomal histone H4 and H2A. The NuA4 complex is also involved in DNA repair (By similarity). Also involved in deacetylation of histones, chromatin assembly and chromosome segregation. May act as a transcriptional oscillator, directing histone deacetylases to specific chromosomal domains. KEYWORDS: 3D-structure;Chromatin regulator;Direct protein sequencing;DNA damage;DNA repair;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " O13961,"PROTEIN NAMES: Nuclear envelope protein ndc1 (Cell untimely torn protein 11) PROTEIN FAMILY: NDC1 family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the NDC1 family. FUNCTION: Component of the nuclear pore complex (NPC) and the spindle pole body (SPB), which plays a key role in de novo assembly and insertion of both structures in the nuclear envelope. Involved in the formation of the bipolar mitotic spindle. Anchors the spindle pole body in the nuclear envelope. KEYWORDS: Cytoplasm;Cytoskeleton;Membrane;mRNA transport;Nuclear pore complex;Nucleus;Protein transport;Reference proteome;Translocation;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Nucleus, nuclear pore complex. Nucleus membrane; Multi-pass membrane protein. Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body. Note=Central core structure of the nuclear pore complex. " O14008,"PROTEIN NAMES: Myosin 1 light chain cam2 (Calmodulin-2) PROTEIN FAMILY: Calmodulin family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the calmodulin family. FUNCTION: Plays a role in meiosis and sporulation. KEYWORDS: Cytoplasm;Meiosis;Membrane;Reference proteome;Repeat;Sporulation SUBCELLULAR LOCATION: Cytoplasm Prospore membrane. Note=Accumulates at the cell poles in interphase cells and at the medial septation site in post-mitotic cells, colocalizing with myo1 and F-actin patches. During the mating process, a single dot is detected a the tip of the mating projection. During meiosis I, dots disperse into the cell periphery and the cytoplasm. At metaphase II, intense signals appear near meu14 rings which are formed at the leading edge of expanding forespore membranes. Localization is dependent upon myo1 with the exception of the localization to mating projections. " O14011,PROTEIN NAMES: Pre-mRNA-processing factor 19 (Complexed with cdc5 protein 8) (RING-type E3 ubiquitin transferase PRP19) PROTEIN FAMILY: WD repeat PRP19 family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the WD repeat PRP19 family. FUNCTION: Probable ubiquitin-protein ligase involved in pre-mRNA splicing (By similarity). May also function in DNA repair (By similarity). PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: 3D-structure;Direct protein sequencing;DNA damage;DNA repair;mRNA processing;mRNA splicing;Nucleus;Reference proteome;Repeat;Spliceosome;Transferase;Ubl conjugation pathway;WD repeat SUBCELLULAR LOCATION: Nucleus O14021,PROTEIN NAMES: RbAp48-related WD40 repeat-containing protein prw1 PROTEIN FAMILY: WD repeat HIR1 family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the WD repeat HIR1 family. FUNCTION: Has a role in chromatin assembly and chromosome segregation. Involved in the deacetylation of histones. KEYWORDS: 3D-structure;Chromatin regulator;Direct protein sequencing;Nucleus;Reference proteome;Repeat;Transcription;Transcription regulation;WD repeat SUBCELLULAR LOCATION: Nucleus O14031,PROTEIN NAMES: Glutathione transporter 1 PROTEIN FAMILY: Oligopeptide OPT transporter family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the oligopeptide OPT transporter family. FUNCTION: High-affinity glutathione transporter which plays a role in scavenging glutathione from the extracellular environment for the maintenance of sulfur homeostasis. KEYWORDS: Cell membrane;Coiled coil;Endoplasmic reticulum;Glycoprotein;Membrane;Peptide transport;Phosphoprotein;Protein transport;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. O14050,PROTEIN NAMES: Mechanosensitive ion channel protein Msy2 PROTEIN FAMILY: MscS family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the MscS (TC 1.A.23) family. FUNCTION: Regulates intracellular calcium levels and cell volume for survival in response to hypo-osmotic shock. Involved in maintaining vacuole integrity and protecting the nuclear envelope upon hypo-osmotic shock. KEYWORDS: Endoplasmic reticulum;Membrane;Reference proteome;Stress response;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein Note=Cortical endoplasmic reticulum. O14079,"PROTEIN NAMES: DNA damage response protein Mdb1 (BRCT domain protein Mdb1) (Midzone and DNA break-localizing protein 1) ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) FUNCTION: Involved in DNA damage response (DDR) mediated through its interaction with phosphorylated H2A proteins hta1 and hta2 which mark the discrete foci of DNA damage. KEYWORDS: 3D-structure;Cell cycle;Chromosome;Cytoplasm;Cytoskeleton;DNA damage;Nucleus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Nucleus Chromosome Cytoplasm, cytoskeleton, spindle Note=Associated with chromatin. Relocalizes to discrete nuclear foci at DNA double strand breaks (DSBs) following DNA damage by the HO endonuclease and ionizing radiation (IR). Focus formation requires interaction with phosphorylated hta1 and hta2. During mitosis, localizes to spindles and concentrates at spindle midzones at late mitosis. Localization to spindle midzones requires ase1, but does not require phosphorylated hta1 nor hta2. Localizes to spindle midzones in anaphase. " O14129,"PROTEIN NAMES: DNA repair protein rhp55 (RAD55 homolog) PROTEIN FAMILY: RecA family, RAD55 subfamily ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the RecA family. RAD55 subfamily. FUNCTION: Required for radiation resistance and meiotic viability and acts in recombination and recombinational DNA repair pathways. KEYWORDS: ATP-binding;DNA damage;DNA repair;Nucleotide-binding;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus " O14213,PROTEIN NAMES: ER-phagy receptor 1 (Meiotically up-regulated gene 185 protein) ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) FUNCTION: Reticulophagy receptor required for autophagosomal sequestration of endoplasmic reticulum (ER) membranes during ER stress (Ref.5). Confers resistance to ER stress by promoting the autophagic degradation of the ER (ER-phagy or reticulophagy) (Ref.5). Acts as a bridging molecule to mediate the association between atg8 on the autophagic membrane and the vesicle-associated membrane protein-associated proteins (VAPs) scs2 and scs22 on the ER (Ref.5). May play a role in meiosis. KEYWORDS: Endoplasmic reticulum;Meiosis;Metal-binding;Phosphoprotein;Reference proteome;Zinc;Zinc-finger SUBCELLULAR LOCATION: Endoplasmic reticulum Preautophagosomal structure Note=Accumulates at the preautophagosomal structure when autophagy occurs. O14261,"PROTEIN NAMES: Taz1-interacting factor 1 (Protein taf1) (Autophagy-related protein 11) (Cytoplasm to vacuole targeting protein 9) PROTEIN FAMILY: ATG11 family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the ATG11 family. FUNCTION: Involved in cytoplasm to vacuole transport (Cvt), pexophagy, mitophagy and nucleophagy. Recruits mitochondria for their selective degradation via autophagy (mitophagy) during starvation. Works as scaffold proteins that recruit ATG proteins to the preautophagosome (PAS), the site of vesicle/autophagosome formation. Required for atg9 anterograde transport from the mitochondria to the PAS (By similarity). Required for nitrogen starvation-induced sexual development and for entering the dormant G0 state (By similarity). KEYWORDS: Autophagy;Coiled coil;Membrane;Phosphoprotein;Protein transport;Reference proteome;Transport;Vacuole SUBCELLULAR LOCATION: Preautophagosomal structure membrane ; Peripheral membrane protein Vacuole membrane ; Peripheral membrane protein " O14283,PROTEIN NAMES: Transcription factor prr1 (Pombe response regulator 1) PROTEIN FAMILY: HSF family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: In the N-terminal section; belongs to the HSF family. FUNCTION: Involved in oxidative stress. Transcription factor that acts upon trr1 and ctt1. KEYWORDS: DNA-binding;Nucleus;Phosphoprotein;Reference proteome;Sensory transduction;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus O14295,PROTEIN NAMES: Pyridoxal reductase (PL reductase) (PL-red) PROTEIN FAMILY: Aldo/keto reductase family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the aldo/keto reductase family. FUNCTION: Catalyzes the reduction of pyridoxal (PL) with NADPH and oxidation of pyridoxine (PN) with NADP(+). PATHWAY: Cofactor degradation; B6 vitamer degradation; pyridoxal from pyridoxine (dehydrogenase route): step 1/1. KEYWORDS: Cytoplasm;Direct protein sequencing;NADP;Oxidoreductase;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cytoplasm O14305,"PROTEIN NAMES: Serine/threonine-protein kinase sid1 (STE20-like kinase sid1) PROTEIN FAMILY: Protein kinase superfamily, STE Ser/Thr protein kinase family, STE20 subfamily ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. FUNCTION: Has a role in the septation initiation network (SIN) required for cytokinesis. KEYWORDS: ATP-binding;Cell cycle;Cell division;Cytoplasm;Cytoskeleton;Kinase;Mitosis;Nucleotide-binding;Reference proteome;Serine/threonine-protein kinase;Transferase SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body Note=Localizes to the SPB prior to cytokinesis and leaves once septation is complete. " O14333,"PROTEIN NAMES: Phosphatidylserine decarboxylase proenzyme 1, mitochondrial [Cleaved into: Phosphatidylserine decarboxylase 1 beta chain; Phosphatidylserine decarboxylase 1 alpha chain] PROTEIN FAMILY: Phosphatidylserine decarboxylase family, PSD-B subfamily, Eukaryotic type I sub-subfamily ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the phosphatidylserine decarboxylase family. PSD-B subfamily. Eukaryotic type I sub-subfamily. FUNCTION: Catalyzes the formation of phosphatidylethanolamine (PtdEtn) from phosphatidylserine (PtdSer). Plays a central role in phospholipid metabolism and in the interorganelle trafficking of phosphatidylserine (By similarity). Together with psd2 and psd3, responsible for the majority of phosphatidylethanolamine synthesis. PATHWAY: Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from CDP-diacylglycerol: step 2/2. KEYWORDS: Decarboxylase;Lipid biosynthesis;Lipid metabolism;Lyase;Membrane;Mitochondrion;Mitochondrion inner membrane;Phospholipid biosynthesis;Phospholipid metabolism;Pyruvate;Reference proteome;Transit peptide;Transmembrane;Transmembrane helix;Zymogen SUBCELLULAR LOCATION: [Phosphatidylserine decarboxylase 1 beta chain]: Mitochondrion Mitochondrion inner membrane ; Single-pass membrane protein ; Intermembrane side.; SUBCELLULAR LOCATION: [Phosphatidylserine decarboxylase 1 alpha chain]: Mitochondrion Mitochondrion inner membrane ; Peripheral membrane protein ; Intermembrane side Note=Anchored to the mitochondrial inner membrane through its interaction with the integral membrane beta chain. " O14370,"PROTEIN NAMES: Branched-chain-amino-acid aminotransferase, mitochondrial (BCAT) PROTEIN FAMILY: Class-IV pyridoxal-phosphate-dependent aminotransferase family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family. FUNCTION: Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine. KEYWORDS: Amino-acid biosynthesis;Aminotransferase;Branched-chain amino acid biosynthesis;Cytoplasm;Mitochondrion;Nucleus;Pyridoxal phosphate;Reference proteome;Transferase;Transit peptide SUBCELLULAR LOCATION: Mitochondrion Nucleus Cytoplasm " O14470,"PROTEIN NAMES: SWI/SNF and RSC complexes subunit ssr2 PROTEIN FAMILY: SMARCC family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the SMARCC family. FUNCTION: Component of the chromatin structure remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. Controls particularly membrane and organelle development genes. Part of the SWI/SNF complex, an ATP-dependent chromatin remodeling complex, required for the positive and negative regulation of gene expression of a large number of genes. It changes chromatin structure by altering DNA-histone contacts within a nucleosome, leading eventually to a change in nucleosome position, thus facilitating or repressing binding of gene-specific transcription factors. KEYWORDS: Chromatin regulator;Cytoplasm;DNA-binding;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cytoplasm Nucleus " O14523,"PROTEIN NAMES: Phospholipid transfer protein C2CD2L (C2 domain-containing protein 2-like) (C2CD2-like) (Transmembrane protein 24) ORGANISM: Homo sapiens (Human) FUNCTION: Lipid-binding protein that transports phosphatidylinositol, the precursor of phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2), from its site of synthesis in the endoplasmic reticulum to the cell membrane. It thereby maintains the pool of cell membrane phosphoinositides, which are degraded during phospholipase C (PLC) signaling. Plays a key role in the coordination of Ca(2+) and phosphoinositide signaling: localizes to sites of contact between the endoplasmic reticulum and the cell membrane, where it tethers the two bilayers. In response to elevation of cytosolic Ca(2+), it is phosphorylated at its C-terminus and dissociates from the cell membrane, abolishing phosphatidylinositol transport to the cell membrane. Positively regulates insulin secretion in response to glucose: phosphatidylinositol transfer to the cell membrane allows replenishment of PI(4,5)P2 pools and calcium channel opening, priming a new population of insulin granules. KEYWORDS: 3D-structure;Alternative splicing;Cell membrane;Endoplasmic reticulum;Lipid transport;Lipid-binding;Membrane;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Single-pass membrane protein Cell membrane ; Peripheral membrane protein Note=Localizes to sites of contact between the endoplasmic reticulum and the cell membrane. Embedded into the endoplasmic reticulum membrane via its N-terminal transmembrane domain and associates with cell membrane via its C-terminus. In response to elevation of cytosolic Ca(2+), it is phosphorylated at its C-terminus and dissociates from the cell membrane and localizes to the reticular endoplasmic reticulum. Reassociates with cell membrane upon dephosphorylation. " O14618,PROTEIN NAMES: Copper chaperone for superoxide dismutase (Superoxide dismutase copper chaperone) PROTEIN FAMILY: Cu-Zn superoxide dismutase family ORGANISM: Homo sapiens (Human) SIMILARITY: In the C-terminal section; belongs to the Cu-Zn superoxide dismutase family. FUNCTION: Delivers copper to copper zinc superoxide dismutase (SOD1). KEYWORDS: 3D-structure;Chaperone;Copper;Cytoplasm;Disulfide bond;Isopeptide bond;Metal-binding;Phosphoprotein;Reference proteome;Ubl conjugation;Zinc SUBCELLULAR LOCATION: Cytoplasm O14653,"PROTEIN NAMES: Golgi SNAP receptor complex member 2 (27 kDa Golgi SNARE protein) (Membrin) PROTEIN FAMILY: GOSR2 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the GOSR2 family. FUNCTION: Involved in transport of proteins from the cis/medial-Golgi to the trans-Golgi network. KEYWORDS: 3D-structure;Acetylation;Alternative splicing;Coiled coil;Congenital muscular dystrophy;Direct protein sequencing;Disease variant;Endoplasmic reticulum;Epilepsy;Golgi apparatus;Membrane;Neurodegeneration;Protein transport;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Golgi apparatus, cis-Golgi network membrane ; Single-pass type IV membrane protein Golgi apparatus membrane Endoplasmic reticulum membrane Note=Concentrated most in the intermediate compartment/cis-Golgi network and the cis-Golgi cisternae 1 and 2. Greatly reduced in concentration at the trans end of the Golgi apparatus. " O14681,PROTEIN NAMES: Etoposide-induced protein 2.4 homolog (p53-induced gene 8 protein) PROTEIN FAMILY: EI24 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the EI24 family. FUNCTION: Acts as a negative growth regulator via p53-mediated apoptosis pathway. Regulates formation of degradative autolysosomes during autophagy (By similarity). KEYWORDS: Acetylation;Alternative splicing;Apoptosis;Autophagy;Cytoplasm;Disease variant;Endoplasmic reticulum;Membrane;Nucleus;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Nucleus membrane ; Multi-pass membrane protein Cytoplasm Endoplasmic reticulum membrane ; Multi-pass membrane protein O14713,"PROTEIN NAMES: Integrin beta-1-binding protein 1 (Integrin cytoplasmic domain-associated protein 1) (ICAP-1) ORGANISM: Homo sapiens (Human) FUNCTION: Key regulator of the integrin-mediated cell-matrix interaction signaling by binding to the ITGB1 cytoplasmic tail and preventing the activation of integrin alpha-5/beta-1 (heterodimer of ITGA5 and ITGB1) by talin or FERMT1. Plays a role in cell proliferation, differentiation, spreading, adhesion and migration in the context of mineralization and bone development and angiogenesis. Stimulates cellular proliferation in a fibronectin-dependent manner. Involved in the regulation of beta-1 integrin-containing focal adhesion (FA) site dynamics by controlling its assembly rate during cell adhesion; inhibits beta-1 integrin clustering within FA by directly competing with talin TLN1, and hence stimulates osteoblast spreading and migration in a fibronectin- and/or collagen-dependent manner. Acts as a guanine nucleotide dissociation inhibitor (GDI) by regulating Rho family GTPases during integrin-mediated cell matrix adhesion; reduces the level of active GTP-bound form of both CDC42 and RAC1 GTPases upon cell adhesion to fibronectin. Stimulates the release of active CDC42 from the membranes to maintain it in an inactive cytoplasmic pool. Participates in the translocation of the Rho-associated protein kinase ROCK1 to membrane ruffles at cell leading edges of the cell membrane, leading to an increase of myoblast cell migration on laminin. Plays a role in bone mineralization at a late stage of osteoblast differentiation; modulates the dynamic formation of focal adhesions into fibrillar adhesions, which are adhesive structures responsible for fibronectin deposition and fibrillogenesis. Plays a role in blood vessel development; acts as a negative regulator of angiogenesis by attenuating endothelial cell proliferation and migration, lumen formation and sprouting angiogenesis by promoting AKT phosphorylation and inhibiting ERK1/2 phosphorylation through activation of the Notch signaling pathway. Promotes transcriptional activity of the MYC promoter. KEYWORDS: 3D-structure;Alternative splicing;Angiogenesis;Biomineralization;Cell adhesion;Cell membrane;Cell projection;Cytoplasm;Cytoskeleton;Differentiation;Membrane;Mitogen;Notch signaling pathway;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Cytoplasm, cytoskeleton Cell membrane Cell projection, lamellipodium. Cell projection, ruffle. Note=Nucleocytoplasmic shuttling protein; shuttles between nucleus and cytoplasm in a integrin-dependent manner; probably sequestered in the cytosol by ITGB1. Its localization is dependent on the stage of cell spreading on fibronectin; cytoplasmic in case of round cells, corresponding to the initial step of cell spreading, or nuclear in case of well spread cells. Colocalizes with ROCK1 and NME2 at beta-1 integrin engagement sites. Together with ITGB1 and NME2 is recruited to beta-1 integrin-rich peripheral ruffles and lamellipodia during initial cell spreading on fibronectin and/or collagen. MISCELLANEOUS: [Isoform 1]: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.; MISCELLANEOUS: [Isoform 2]: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay." O14730,"PROTEIN NAMES: Serine/threonine-protein kinase RIO3 (RIO kinase 3) (sudD homolog) PROTEIN FAMILY: Protein kinase superfamily, RIO-type Ser/Thr kinase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the protein kinase superfamily. RIO-type Ser/Thr kinase family. FUNCTION: Involved in regulation of type I interferon (IFN)-dependent immune response which plays a critical role in the innate immune response against DNA and RNA viruses. May act as an adapter protein essential for the recruitment of TBK1 to IRF3. Phosphorylates IFIH1 on 'Ser-828' interfering with IFIH1 filament assembly on long dsRNA and resulting in attenuated IFIH1-signaling. Can inhibit CASP10 isoform 7-mediated activation of the NF-kappaB signaling pathway. May play a role in the biogenesis of the 40S ribosomal subunit. Involved in the processing of 21S pre-rRNA to the mature 18S rRNA. KEYWORDS: Alternative splicing;Antiviral defense;ATP-binding;Cytoplasm;Immunity;Innate immunity;Kinase;Magnesium;Metal-binding;Nucleotide-binding;Phosphoprotein;Reference proteome;Ribosome biogenesis;Serine/threonine-protein kinase;Transferase SUBCELLULAR LOCATION: Cytoplasm " O14772,PROTEIN NAMES: Fucose-1-phosphate guanylyltransferase (GDP-L-fucose diphosphorylase) (GDP-L-fucose pyrophosphorylase) ORGANISM: Homo sapiens (Human) FUNCTION: Catalyzes the formation of GDP-L-fucose from GTP and L-fucose-1-phosphate. Functions as a salvage pathway to reutilize L-fucose arising from the turnover of glycoproteins and glycolipids. KEYWORDS: Alternative splicing;Cytoplasm;GTP-binding;Nucleotide-binding;Nucleotidyltransferase;Reference proteome;Transferase SUBCELLULAR LOCATION: Cytoplasm. O14791,PROTEIN NAMES: Apolipoprotein L1 (Apolipoprotein L) (Apo-L) (ApoL) (Apolipoprotein L-I) (ApoL-I) PROTEIN FAMILY: Apolipoprotein L family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the apolipoprotein L family. FUNCTION: May play a role in lipid exchange and transport throughout the body. May participate in reverse cholesterol transport from peripheral cells to the liver. KEYWORDS: 3D-structure;Alternative splicing;Cholesterol metabolism;Direct protein sequencing;Disease variant;Glycoprotein;HDL;Lipid metabolism;Lipid transport;Phosphoprotein;Reference proteome;Secreted;Signal;Steroid metabolism;Sterol metabolism;Transport SUBCELLULAR LOCATION: Secreted. MISCELLANEOUS: [Isoform 1]: Major isoform. O14804,"PROTEIN NAMES: Trace amine-associated receptor 5 (TaR-5) (Trace amine receptor 5) (hTaar5) (Putative neurotransmitter receptor) PROTEIN FAMILY: G-protein coupled receptor 1 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the G-protein coupled receptor 1 family. FUNCTION: Olfactory receptor specific for trimethylamine, a trace amine. Also activated at lower level by dimethylethylamine. Trimethylamine is a bacterial metabolite found in some animal odors, and to humans it is a repulsive odor associated with bad breath and spoiled food. This receptor is probably mediated by the G(s)-class of G-proteins which activate adenylate cyclase. KEYWORDS: Cell membrane;Disulfide bond;G-protein coupled receptor;Glycoprotein;Membrane;Receptor;Reference proteome;Transducer;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein MISCELLANEOUS: Polymorphic variants of this gene are not associated with specific anosmia for trimethylamine." O14829,"PROTEIN NAMES: Serine/threonine-protein phosphatase with EF-hands 1 (PPEF-1) (Protein phosphatase with EF calcium-binding domain) (PPEF) (Serine/threonine-protein phosphatase 7) (PP7) PROTEIN FAMILY: PPP phosphatase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the PPP phosphatase family. FUNCTION: May have a role in the recovery or adaptation response of photoreceptors. May have a role in development. KEYWORDS: Alternative splicing;Calcium;Hydrolase;Magnesium;Manganese;Metal-binding;Protein phosphatase;Reference proteome;Repeat MISCELLANEOUS: [Isoform 2]: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.; MISCELLANEOUS: [Isoform 3]: May have no functional significance." O14832,"PROTEIN NAMES: Phytanoyl-CoA dioxygenase, peroxisomal (Phytanic acid oxidase) (Phytanoyl-CoA alpha-hydroxylase) (PhyH) PROTEIN FAMILY: PhyH family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the PhyH family. FUNCTION: Catalyzes the 2-hydroxylation of not only racemic phytanoyl-CoA and the isomers of 3-methylhexadecanoyl-CoA, but also a variety of other mono-branched 3-methylacyl-CoA esters (with a chain length of at least seven carbon atoms) and straight-chain acyl-CoA esters (with a chain length longer than four carbon atoms). Does not hydroxylate long and very long straight chain acyl-CoAs or 2-methyl- and 4-methyl-branched acyl-CoAs. PATHWAY: Lipid metabolism; fatty acid metabolism. KEYWORDS: 3D-structure;Alternative splicing;Cataract;Deafness;Dioxygenase;Disease variant;Ichthyosis;Iron;Metal-binding;Oxidoreductase;Peroxisome;Peroxisome biogenesis disorder;Phosphoprotein;Reference proteome;Retinitis pigmentosa;Transit peptide;Vitamin C SUBCELLULAR LOCATION: Peroxisome " O14893,"PROTEIN NAMES: Gem-associated protein 2 (Gemin-2) (Component of gems 2) (Survival of motor neuron protein-interacting protein 1) (SMN-interacting protein 1) PROTEIN FAMILY: Gemin-2 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the gemin-2 family. FUNCTION: The SMN complex catalyzes the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome, and thereby plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP (Sm core). In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG (5Sm) are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. To assemble core snRNPs, the SMN complex accepts the trapped 5Sm proteins from CLNS1A. Binding of snRNA inside 5Sm ultimately triggers eviction of the SMN complex, thereby allowing binding of SNRPD3 and SNRPB to complete assembly of the core snRNP. Within the SMN complex, GEMIN2 constrains the conformation of 5Sm, thereby promoting 5Sm binding to snRNA containing the snRNP code (a nonameric Sm site and a 3'-adjacent stem-loop), thus preventing progression of assembly until a cognate substrate is bound. KEYWORDS: 3D-structure;Alternative initiation;Alternative splicing;Cytoplasm;mRNA processing;mRNA splicing;Nucleus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Nucleus, gem. Cytoplasm. Note=Localized in subnuclear structures next to coiled bodies, called gems, which are highly enriched in spliceosomal snRNPs. Also found in the cytoplasm. " O14901,"PROTEIN NAMES: Krueppel-like factor 11 (Transforming growth factor-beta-inducible early growth response protein 2) (TGFB-inducible early growth response protein 2) (TIEG-2) PROTEIN FAMILY: Sp1 C2H2-type zinc-finger protein family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the Sp1 C2H2-type zinc-finger protein family. FUNCTION: Transcription factor. Activates the epsilon- and gamma-globin gene promoters and, to a much lower degree, the beta-globin gene and represses promoters containing SP1-like binding inhibiting cell growth. Represses transcription of SMAD7 which enhances TGF-beta signaling (By similarity). Induces apoptosis (By similarity). KEYWORDS: Activator;Alternative splicing;Apoptosis;Diabetes mellitus;Disease variant;DNA-binding;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Repeat;Repressor;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus " O14904,"PROTEIN NAMES: Protein Wnt-9a (Protein Wnt-14) PROTEIN FAMILY: Wnt family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the Wnt family. FUNCTION: Ligand for members of the frizzled family of seven transmembrane receptors. Functions in the canonical Wnt/beta-catenin signaling pathway. Required for normal timing of IHH expression during embryonic bone development, normal chondrocyte maturation and for normal bone mineralization during embryonic bone development. Plays a redundant role in maintaining joint integrity. KEYWORDS: Developmental protein;Disulfide bond;Extracellular matrix;Glycoprotein;Lipoprotein;Reference proteome;Secreted;Signal;Wnt signaling pathway SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix Secreted " O14908,PROTEIN NAMES: PDZ domain-containing protein GIPC1 (GAIP C-terminus-interacting protein) (RGS-GAIP-interacting protein) (RGS19-interacting protein 1) (Synectin) (Tax interaction protein 2) (TIP-2) PROTEIN FAMILY: GIPC family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the GIPC family. FUNCTION: May be involved in G protein-linked signaling. KEYWORDS: Alternative splicing;Congenital muscular dystrophy;Cytoplasm;Dystroglycanopathy;Membrane;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Membrane ; Peripheral membrane protein. O14931,"PROTEIN NAMES: Natural cytotoxicity triggering receptor 3 (Activating natural killer receptor p30) (Natural killer cell p30-related protein) (NK-p30) (NKp30) (CD antigen CD337) PROTEIN FAMILY: Natural cytotoxicity receptor (NCR) family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the natural cytotoxicity receptor (NCR) family. FUNCTION: Cell membrane receptor of natural killer/NK cells that is activated by binding of extracellular ligands including BAG6 and NCR3LG1. Stimulates NK cells cytotoxicity toward neighboring cells producing these ligands. It controls, for instance, NK cells cytotoxicity against tumor cells. Engagement of NCR3 by BAG6 also promotes myeloid dendritic cells (DC) maturation, both through killing DCs that did not acquire a mature phenotype, and inducing the release by NK cells of TNFA and IFNG which promote DC maturation. KEYWORDS: 3D-structure;Alternative splicing;Cell membrane;Disulfide bond;Glycoprotein;Immunity;Immunoglobulin domain;Membrane;Receptor;Reference proteome;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein " O15062,PROTEIN NAMES: Zinc finger and BTB domain-containing protein 5 ORGANISM: Homo sapiens (Human) FUNCTION: May be involved in transcriptional regulation. KEYWORDS: DNA-binding;Isopeptide bond;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Repeat;Transcription;Transcription regulation;Ubl conjugation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus O15116,"PROTEIN NAMES: U6 snRNA-associated Sm-like protein LSm1 (Cancer-associated Sm-like) (Small nuclear ribonuclear CaSm) PROTEIN FAMILY: SnRNP Sm proteins family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the snRNP Sm proteins family. FUNCTION: Plays a role in the degradation of histone mRNAs, the only eukaryotic mRNAs that are not polyadenylated. Probably also part of an LSm subunits-containing complex involved in the general process of mRNA degradation (By similarity). KEYWORDS: Cytoplasm;mRNA processing;mRNA splicing;Phosphoprotein;Reference proteome;Ribonucleoprotein;RNA-binding SUBCELLULAR LOCATION: Cytoplasm Cytoplasm, P-body " O15204,PROTEIN NAMES: ADAM DEC1 (A disintegrin and metalloproteinase domain-like protein decysin-1) (ADAM-like protein decysin-1) ORGANISM: Homo sapiens (Human) FUNCTION: May play an important role in the control of the immune response and during pregnancy. KEYWORDS: Alternative splicing;Disulfide bond;Glycoprotein;Hydrolase;Metal-binding;Metalloprotease;Protease;Reference proteome;Secreted;Signal;Zinc;Zymogen SUBCELLULAR LOCATION: Secreted O15229,"PROTEIN NAMES: Kynurenine 3-monooxygenase (Kynurenine 3-hydroxylase) PROTEIN FAMILY: Aromatic-ring hydroxylase family, KMO subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the aromatic-ring hydroxylase family. KMO subfamily. FUNCTION: Catalyzes the hydroxylation of L-kynurenine (L-Kyn) to form 3-hydroxy-L-kynurenine (L-3OHKyn). Required for synthesis of quinolinic acid, a neurotoxic NMDA receptor antagonist and potential endogenous inhibitor of NMDA receptor signaling in axonal targeting, synaptogenesis and apoptosis during brain development. Quinolinic acid may also affect NMDA receptor signaling in pancreatic beta cells, osteoblasts, myocardial cells, and the gastrointestinal tract (Probable). PATHWAY: Cofactor biosynthesis; NAD(+) biosynthesis; quinolinate from L-kynurenine: step 1/3. KEYWORDS: 3D-structure;Alternative splicing;FAD;Flavoprotein;Glycoprotein;Membrane;Mitochondrion;Mitochondrion outer membrane;Monooxygenase;NADP;Oxidoreductase;Pyridine nucleotide biosynthesis;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Mitochondrion outer membrane ; Multi-pass membrane protein MISCELLANEOUS: Increased in neuroinflammatory conditions. Inhibitors are investigated as potential neuroprotective drugs since they lead to an increased level of kynurenic acid, a neuroprotective NMDA receptor agonist.; MISCELLANEOUS: [Isoform 3]: Gene model based on mouse cDNA data." O15231,"PROTEIN NAMES: Zinc finger protein 185 (LIM domain protein ZNF185) (P1-A) ORGANISM: Homo sapiens (Human) FUNCTION: May be involved in the regulation of cellular proliferation and/or differentiation. KEYWORDS: Alternative splicing;Cell junction;Cytoplasm;Cytoskeleton;LIM domain;Metal-binding;Phosphoprotein;Reference proteome;Zinc SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton Cell junction, focal adhesion " O15232,PROTEIN NAMES: Matrilin-3 ORGANISM: Homo sapiens (Human) FUNCTION: Major component of the extracellular matrix of cartilage and may play a role in the formation of extracellular filamentous networks. KEYWORDS: Alternative splicing;Coiled coil;Disease variant;Disulfide bond;Dwarfism;EGF-like domain;Methylation;Phosphoprotein;Reference proteome;Repeat;Secreted;Signal SUBCELLULAR LOCATION: Secreted O15254,PROTEIN NAMES: Peroxisomal acyl-coenzyme A oxidase 3 (Branched-chain acyl-CoA oxidase) (BRCACox) (Pristanoyl-CoA oxidase) PROTEIN FAMILY: Acyl-CoA oxidase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the acyl-CoA oxidase family. FUNCTION: Oxidizes the CoA-esters of 2-methyl-branched fatty acids. PATHWAY: Lipid metabolism; peroxisomal fatty acid beta-oxidation. KEYWORDS: Acetylation;Alternative splicing;FAD;Fatty acid metabolism;Flavoprotein;Lipid metabolism;Oxidoreductase;Peroxisome;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Peroxisome O15259,"PROTEIN NAMES: Nephrocystin-1 (Juvenile nephronophthisis 1 protein) PROTEIN FAMILY: Nephrocystin-1 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the nephrocystin-1 family. FUNCTION: Together with BCAR1 it may play a role in the control of epithelial cell polarity (By similarity). Involved in the organization of apical junctions in kidney cells together with NPHP4 and RPGRIP1L/NPHP8 (By similarity). Does not seem to be strictly required for ciliogenesis (By similarity). Seems to help to recruit PTK2B/PYK2 to cell matrix adhesions, thereby initiating phosphorylation of PTK2B/PYK2 and PTK2B/PYK2-dependent signaling (By similarity). May play a role in the regulation of intraflagellar transport (IFT) during cilia assembly. Required for normal retina development (By similarity). In connecting photoreceptor cilia influences the movement of some IFT proteins such as IFT88 and WDR19. Involved in spermatogenesis (By similarity). KEYWORDS: 3D-structure;Alternative splicing;Cell junction;Cell projection;Ciliopathy;Cilium;Cilium biogenesis/degradation;Coiled coil;Cytoplasm;Cytoskeleton;Differentiation;Disease variant;Joubert syndrome;Leber congenital amaurosis;Nephronophthisis;Phosphoprotein;Reference proteome;Senior-Loken syndrome;SH3 domain;Spermatogenesis;Tight junction SUBCELLULAR LOCATION: Cell junction Cell junction, adherens junction Cell projection, cilium Cytoplasm, cytoskeleton, cilium axoneme Cell junction, tight junction. Note=In the retinal photoreceptor cell layer, localizes at the connecting cilium (By similarity). Colocalizes with E-cadherin and BCAR1 at or near the cell-cell adherens junctions (By similarity). Localized to respiratory cilia axoneme. Localized to the transition zone of respiratory cilia. Localized to the transition zone of photoreceptor-connecting cilia and renal monocilia (By similarity). In cultured renal cells, it localizes diffusely in the cytoplasm but, as cells approach confluence, it accumulates at basolateral tight junctions (By similarity). MISCELLANEOUS: Nephronophthisis type 1 patients deficient for NPHP1 show normal overall integrity of respiratory cilia." O15297,"PROTEIN NAMES: Protein phosphatase 1D (Protein phosphatase 2C isoform delta) (PP2C-delta) (Protein phosphatase magnesium-dependent 1 delta) (p53-induced protein phosphatase 1) PROTEIN FAMILY: PP2C family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the PP2C family. FUNCTION: Involved in the negative regulation of p53 expression. Required for the relief of p53-dependent checkpoint mediated cell cycle arrest. Binds to and dephosphorylates 'Ser-15' of TP53 and 'Ser-345' of CHEK1 which contributes to the functional inactivation of these proteins. Mediates MAPK14 dephosphorylation and inactivation. Is also an important regulator of global heterochromatin silencing and critical in maintaining genome integrity (By similarity). KEYWORDS: Alternative splicing;Autism spectrum disorder;Cell cycle;Cytoplasm;Disease variant;Hydrolase;Intellectual disability;Magnesium;Manganese;Metal-binding;Nucleus;Phosphoprotein;Protein phosphatase;Reference proteome SUBCELLULAR LOCATION: Nucleus Cytoplasm, cytosol " O15354,"PROTEIN NAMES: Prosaposin receptor GPR37 (Endothelin B receptor-like protein 1) (ETBR-LP-1) (G-protein coupled receptor 37) (Parkin-associated endothelin receptor-like receptor) (PAELR) PROTEIN FAMILY: G-protein coupled receptor 1 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the G-protein coupled receptor 1 family. FUNCTION: G-protein-coupled receptor that plays a role in several physiological pathways such as resolution of inflammatory pain and oligodendrocyte differentiation (By similarity). Acts as a receptor for several ligands including prosaposin, osteocalcin or neuroprotectin D1. Ligand binding induces endocytosis, followed by an ERK phosphorylation cascade. Acts as a receptor for osteocalcin (OCN) to regulate oligodendrocyte differentiation and central nervous system myelination. Mechanistically, plays a negative role in oligodendrocyte differentiation and myelination during development via activation of the ERK1/2 signaling pathway. Therefore, regulates the stability of myelin or resistance of myelin itself to demyelination. Upon activation by neuroprotectin D1 (NPD1), promotes the activation of phagocytosis in macrophages as well as the shift in cytokine release toward an anti-inflammatory profile, and thus helps to reverse inflammatory pain. In addition, the increased macrophage phagocytosis mediates protection against sepsis upon pathogen infection. Additionally, extracellular vesicles derived from efferocyte express prosaposin, which binds to macrophage GPR37 to increase expression of the efferocytosis receptor TIM4 via an ERK-AP1-dependent signaling axis, leading to increased macrophage efferocytosis efficiency and accelerated resolution of inflammation (By similarity). May also act as a maturation factor of LRP6, protecting LRP6 from the endoplasmic reticulum (ER)-associated protein degradation (ERAD) and thereby promoting the Wnt/beta-catenin signaling pathway. KEYWORDS: Cell membrane;Cell projection;Disulfide bond;Endoplasmic reticulum;G-protein coupled receptor;Glycoprotein;Membrane;Receptor;Reference proteome;Signal;Synapse;Transducer;Transmembrane;Transmembrane helix;Ubl conjugation SUBCELLULAR LOCATION: Cell projection, dendrite Synapse Cell membrane ; Multi-pass membrane protein Endoplasmic reticulum membrane ; Multi-pass membrane protein " O15370,"PROTEIN NAMES: Transcription factor SOX-12 (Protein SOX-22) ORGANISM: Homo sapiens (Human) FUNCTION: Transcription factor that binds to DNA at the consensus sequence 5'-ACCAAAG-3' (By similarity). Acts as a transcriptional activator (By similarity). Binds cooperatively with POU3F2/BRN2 or POU3F1/OCT6 to gene promoters, which enhances transcriptional activation (By similarity). Involved in the differentiation of naive CD4-positive T-cells into peripherally induced regulatory T (pT reg) cells under inflammatory conditions (By similarity). Binds to the promoter region of the FOXP3 gene and promotes its transcription, and might thereby contribute to pT reg cell differentiation in the spleen and lymph nodes during inflammation (By similarity). Plays a redundant role with SOX4 and SOX11 in cell survival of developing tissues such as the neural tube, branchial arches and somites, thereby contributing to organogenesis (By similarity). KEYWORDS: Activator;DNA-binding;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " O15389,"PROTEIN NAMES: Sialic acid-binding Ig-like lectin 5 (Siglec-5) (CD33 antigen-like 2) (Obesity-binding protein 2) (OB-BP2) (OB-binding protein 2) (CD antigen CD170) PROTEIN FAMILY: Immunoglobulin superfamily, SIGLEC (sialic acid binding Ig-like lectin) family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family. FUNCTION: Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Binds equally to alpha-2,3-linked and alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. KEYWORDS: 3D-structure;Cell adhesion;Disulfide bond;Glycoprotein;Immunoglobulin domain;Lectin;Membrane;Reference proteome;Repeat;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. " O15393,"PROTEIN NAMES: Transmembrane protease serine 2 (Serine protease 10) [Cleaved into: Transmembrane protease serine 2 non-catalytic chain; Transmembrane protease serine 2 catalytic chain] PROTEIN FAMILY: Peptidase S1 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the peptidase S1 family. FUNCTION: Plasma membrane-anchored serine protease that cleaves at arginine residues. Participates in proteolytic cascades of relevance for the normal physiologic function of the prostate. Androgen-induced TMPRSS2 activates several substrates that include pro-hepatocyte growth factor/HGF, the protease activated receptor-2/F2RL1 or matriptase/ST14 leading to extracellular matrix disruption and metastasis of prostate cancer cells. In addition, activates trigeminal neurons and contribute to both spontaneous pain and mechanical allodynia (By similarity).; FUNCTION: (Microbial infection) Facilitates human coronaviruses SARS-CoV and SARS-CoV-2 infections via two independent mechanisms, proteolytic cleavage of ACE2 receptor which promotes viral uptake, and cleavage of coronavirus spike glycoproteins which activates the glycoprotein for host cell entry. The cleavage of SARS-COV2 spike glycoprotein occurs between the S2 and S2' site. Upon SARS-CoV-2 infection, increases syncytia formation by accelerating the fusion process. Proteolytically cleaves and activates the spike glycoproteins of human coronavirus 229E (HCoV-229E) and human coronavirus EMC (HCoV-EMC) and the fusion glycoproteins F0 of Sendai virus (SeV), human metapneumovirus (HMPV), human parainfluenza 1, 2, 3, 4a and 4b viruses (HPIV). Essential for spread and pathogenesis of influenza A virus (strains H1N1, H3N2 and H7N9); involved in proteolytic cleavage and activation of hemagglutinin (HA) protein which is essential for viral infectivity. KEYWORDS: 3D-structure;Alternative splicing;Autocatalytic cleavage;Cell membrane;Disulfide bond;Glycoprotein;Host-virus interaction;Hydrolase;Membrane;Protease;Reference proteome;Secreted;Serine protease;Signal-anchor;Transmembrane;Transmembrane helix;Zymogen SUBCELLULAR LOCATION: Cell membrane ; Single-pass type II membrane protein.; SUBCELLULAR LOCATION: [Transmembrane protease serine 2 catalytic chain]: Secreted Note=Activated by cleavage and secreted. " O15488,"PROTEIN NAMES: Glycogenin-2 (GN-2) (GN2) PROTEIN FAMILY: Glycosyltransferase 8 family, Glycogenin subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the glycosyltransferase 8 family. Glycogenin subfamily. FUNCTION: Glycogenin participates in the glycogen biosynthetic process along with glycogen synthase and glycogen branching enzyme. It self-glucosylates, via an inter-subunit mechanism, to form an oligosaccharide primer that serves as substrate for glycogen synthase. PATHWAY: Glycan biosynthesis; glycogen biosynthesis. KEYWORDS: 3D-structure;Alternative splicing;Glycogen biosynthesis;Glycoprotein;Manganese;Metal-binding;Phosphoprotein;Reference proteome;Transferase " O15504,"PROTEIN NAMES: Nucleoporin NUP42 (NLP-1) (NUP42 homolog) (Nucleoporin hCG1) (Nucleoporin-42) (Nucleoporin-like protein 2) ORGANISM: Homo sapiens (Human) FUNCTION: Required for the export of mRNAs containing poly(A) tails from the nucleus into the cytoplasm.; FUNCTION: (Microbial infection) In case of infection by HIV-1, it may participate in the docking of viral Vpr at the nuclear envelope. KEYWORDS: 3D-structure;Alternative splicing;Glycoprotein;Membrane;Metal-binding;mRNA transport;Nuclear pore complex;Nucleus;Phosphoprotein;Protein transport;Reference proteome;Repeat;Translocation;Transport;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus, nuclear pore complex Nucleus membrane ; Peripheral membrane protein; Cytoplasmic side Note=Excluded from the nucleolus. " O15522,PROTEIN NAMES: Homeobox protein Nkx-2.8 (Homeobox protein NK-2 homolog H) PROTEIN FAMILY: NK-2 homeobox family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the NK-2 homeobox family. KEYWORDS: Developmental protein;DNA-binding;Homeobox;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus O15528,"PROTEIN NAMES: 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial (25-OHD-1 alpha-hydroxylase) (25-hydroxyvitamin D(3) 1-alpha-hydroxylase) (VD3 1A hydroxylase) (Calcidiol 1-monooxygenase) (Cytochrome P450 subfamily XXVIIB polypeptide 1) (Cytochrome P450C1 alpha) (Cytochrome P450VD1-alpha) (Cytochrome p450 27B1) PROTEIN FAMILY: Cytochrome P450 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the cytochrome P450 family. FUNCTION: A cytochrome P450 monooxygenase involved in vitamin D metabolism and in calcium and phosphorus homeostasis. Catalyzes the rate-limiting step in the activation of vitamin D in the kidney, namely the hydroxylation of 25-hydroxyvitamin D3/calcidiol at the C1alpha-position to form the hormonally active form of vitamin D3, 1alpha,25-dihydroxyvitamin D3/calcitriol that acts via the vitamin D receptor (VDR). Has 1alpha-hydroxylase activity on vitamin D intermediates of the CYP24A1-mediated inactivation pathway. Converts 24R,25-dihydroxyvitamin D3/secalciferol to 1-alpha,24,25-trihydroxyvitamin D3, an active ligand of VDR. Also active on 25-hydroxyvitamin D2. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via FDXR/adrenodoxin reductase and FDX1/adrenodoxin. PATHWAY: Hormone biosynthesis; vitamin D biosynthesis. KEYWORDS: Disease variant;Heme;Iron;Lipid metabolism;Membrane;Metal-binding;Mitochondrion;Monooxygenase;Oxidoreductase;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Mitochondrion membrane. " O15541,"PROTEIN NAMES: E3 ubiquitin-protein ligase RNF113A (Cwc24 homolog) (RING finger protein 113A) (Zinc finger protein 183) ORGANISM: Homo sapiens (Human) FUNCTION: Required for pre-mRNA splicing as component of the spliceosome. As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs (Probable). E3 ubiquitin-protein ligase that catalyzes the transfer of ubiquitin onto target proteins. Catalyzes polyubiquitination of SNRNP200/BRR2 with non-canonical 'Lys-63'-linked polyubiquitin chains. Plays a role in DNA repair via its role in the synthesis of 'Lys-63'-linked polyubiquitin chains that recruit ALKBH3 and the ASCC complex to sites of DNA damage by alkylating agents. Ubiquitinates CXCR4, leading to its degradation, and thereby contributes to the termination of CXCR4 signaling. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: 3D-structure;Acetylation;DNA damage;DNA repair;Metal-binding;mRNA processing;mRNA splicing;Nucleus;Phosphoprotein;Reference proteome;Spliceosome;Transferase;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Nucleus speckle Note=Colocalizes with ASCC2 in nuclear foci after DNA damage by alkylating agents. In the absence of DNA damage, colocalizes with the spliceosome components SNRNP200/BRR2 and PRPF8 in nuclear speckles. " O15547,PROTEIN NAMES: P2X purinoceptor 6 (P2X6) (ATP receptor) (P2XM) (Purinergic receptor) (Purinergic receptor P2X-like 1) PROTEIN FAMILY: P2X receptor family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the P2X receptor family. FUNCTION: Receptor for ATP that acts as a ligand-gated ion channel. KEYWORDS: Alternative splicing;Disulfide bond;Glycoprotein;Ion channel;Ion transport;Ligand-gated ion channel;Membrane;Phosphoprotein;Receptor;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. O15554,"PROTEIN NAMES: Intermediate conductance calcium-activated potassium channel protein 4 (SK4) (SKCa 4) (SKCa4) (IKCa1) (IK1) (KCa3.1) (KCa4) (Putative Gardos channel) PROTEIN FAMILY: Potassium channel KCNN family, KCa3.1/KCNN4 subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the potassium channel KCNN family. KCa3.1/KCNN4 subfamily. FUNCTION: Forms a voltage-independent potassium channel that is activated by intracellular calcium. Activation is followed by membrane hyperpolarization which promotes calcium influx. Required for maximal calcium influx and proliferation during the reactivation of naive T-cells. Plays a role in the late stages of EGF-induced macropinocytosis. KEYWORDS: 3D-structure;Calmodulin-binding;Cell membrane;Disease variant;Hereditary hemolytic anemia;Immunity;Ion channel;Ion transport;Membrane;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein " O15706,PROTEIN NAMES: Vacuolin-A PROTEIN FAMILY: Vacuolin family ORGANISM: Dictyostelium discoideum (Social amoeba) SIMILARITY: Belongs to the vacuolin family. KEYWORDS: Coiled coil;Endosome;Lysosome;Membrane;Reference proteome SUBCELLULAR LOCATION: Endosome membrane; Peripheral membrane protein. Lysosome. Note=Post-lysosome. O15945,PROTEIN NAMES: Aryl hydrocarbon receptor nuclear translocator homolog (dARNT) (Hypoxia-inducible factor 1-beta) (Protein tango) ORGANISM: Drosophila melanogaster (Fruit fly) FUNCTION: Heterodimers of tgo/trh are involved in the control of breathless expression. Plays a role in the cellular or tissue response to oxygen deprivation. KEYWORDS: Activator;DNA-binding;Nucleus;Reference proteome;Repeat;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus O16374,"PROTEIN NAMES: Beta-1,4-galactosyltransferase galt-1 PROTEIN FAMILY: Glycosyltransferase 92 family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the glycosyltransferase 92 family. FUNCTION: Catalyzes the transfer of beta-galactose from UDP-galactose to position 4 of alpha-1,6-linked fucose at the reducing end GlcNAc in N-glycan cores. Involved in susceptibility to the nematotoxic C.cinerea galectin Cgl2, likely by contributing to the synthesis of core alpha-1,6-fucosylated N-glycans to which Cgl2 binds. KEYWORDS: Glycoprotein;Glycosyltransferase;Golgi apparatus;Manganese;Membrane;Metal-binding;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus, Golgi stack membrane ; Single-pass type II membrane protein Note=Localizes to vesicles or organelles in coelomocytes. " O16785,"PROTEIN NAMES: Paralyzed arrest at two-fold protein 6 (Actopaxin homolog) (Parvin-like protein) PROTEIN FAMILY: Parvin family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the parvin family. FUNCTION: Involved in the regulation of cell adhesion and cytoskeleton organization. Component of an integrin containing attachment complex, which is required for muscle development and maintenance. During embryonic development, required to recruit cpna-1, unc-89 and myofilaments to newly forming integrin attachments composed of integrins pat-2/pat-3, pat-4 and unc-112. Also required to reposition the integrin-based attachments so that they form the highly ordered array of dense body and M-line attachments that are characteristic of mature muscle cells. During the formation of neuromuscular junctions at the larval stage, negatively regulates membrane protrusion from body wall muscles. KEYWORDS: Actin-binding;Cell adhesion;Cell projection;Cytoplasm;Cytoskeleton;Reference proteome;Repeat SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton Cytoplasm, myofibril, sarcomere, M line Perikaryon Cell projection, axon Note=Colocalizes with integrins and pat-4/ILK. Colocalizes to M line and dense bodies with cpna-1. " O16850,"PROTEIN NAMES: Forkhead box protein O (FOXO) (Abnormal dauer formation protein 16) ORGANISM: Caenorhabditis elegans FUNCTION: Forkhead-type transcription factor. Binds to the promoters of genes that contain the daf-16/FOXO binding element (DBE), TTGTTTAC, in their regulatory region. Functions in the Insulin/IGF-1-like signaling (IIS) mediated pathway which affects lipogenesis, lifespan, starvation survival, heat shock and oxidative stress responses, sleep, associative memory, and dauer formation. Longevity signaling predominantly arises from expression in the intestine. Transcriptional activity of daf-16/FOXO is negatively regulated by interaction with host cell factor homolog hcf-1; and by cytoplasmic sequestration by association with ftt-2. Inhibition is required for the carbon dioxide (CO2) avoidance response. Upon loss of inhibition, daf-16 translocates to the nucleus to regulate genes that result in delayed reproduction and growth while increasing stress resistance starvation tolerance and longevity. Association with arginine methyltransferase prmt-1 prevents phosphorylation and allows for translocation to the nucleus and the subsequent transcription of longevity-related genes. Modulation of its activity by cGMP levels in sensory neurons regulates lifespan. Has a protective role against muscle dystrophy. Involved in mediating protection against aberrant protein aggregation proteotoxicity. Influences transcription of genes that code for proteins involved in immunity as part of a general stress response. Targets genes that inhibit and stimulate tumor growth. Targets kinases, phosphatases and transcription factors that are primarily involved in signaling and gene regulation. Thought to regulate ins-7 in FOXO-to-FOXO signaling, which coordinates daf-16 expression. Activity is positively regulated by shc-1-mediated inhibition of daf-2 and activation of JNK pathway. Through the regulation of its activity by shc-1-mediated inhibition of daf-2 and activation of JNK pathway, plays a role in maintaining the integrity of the gonad. Functions by indirect interaction with jnk-1 of the mitogen-activated protein kinase (MAPK) pathway. Involved in increased proteasome activity by activating expression of rpn-6.1 in response to proteotoxic stress, leading to enhanced assembly of the 26S proteasome, followed by higher proteasome activity. Also regulates proteasome activity in the intestine by preventing expression of deubiquitinase ubh-4. Represses transcription of natc-1. Involved in regulation of srh-234 expression. Binds to the promoter of the AMPK-gamma regulatory subunit, aakg-4, and activates its transcription. Also activates transcription of AMPK-gamma regulatory subunit, aakg-1. Maintains endoplasmic reticulum (ER) function by inducing protein degradation and elimination to remove misfolded secretory proteins from the ER independently of the ire-1/xbp-1 unfolded protein response pathway. Regulates epidermal innate immunity to nematophagous fungal infection and physical wounding which trigger bli-3 induced ROS release, leading to daf-16 activation independently of daf-2 signaling. May negatively regulate resistance to stress caused by oxidized cholesterol adducts by preventing the activation of daf-9 and nuclear hormone receptor daf-12, two members of the steroid signaling pathway. Promotes apoptosis during embryonic development. Probably through the regulation of the autophagy genes atg-18 and atg-16.2, plays a role in regulating stem cell number in the germline during larval development. Plays a role in learning and memory; including associative memory, and aversive gustatory associated learning known as salt avoidance learning. Plays a role in regulating gene transcription in response to white light exposure. Binds to the promoter of dex-1 to positively regulate its expression in seam cells during the dauer phase. Plays a role in transgenerational lipid accumulation in response to a high-fat diet.; FUNCTION: [Isoform a]: Functions in the Insulin/IGF-1-like signaling (IIS) mediated pathway. May play a role in lifespan modulation, but less significant than that played by isoforms d and f.; FUNCTION: [Isoform d]: Functions in the Insulin/IGF-1-like signaling (IIS) mediated pathway. Transcript level in the early adult may play a role in lifespan modulation, but effect is more significant than that played by isoform a.; FUNCTION: [Isoform f]: Functions in the Insulin/IGF-1-like signaling (IIS) mediated pathway. Transcript level in the early adult may play a role in lifespan modulation, but effect is more significant than that played by isoform a. KEYWORDS: Alternative splicing;Cytoplasm;Developmental protein;DNA-binding;Growth regulation;Immunity;Innate immunity;Nucleus;Phosphoprotein;Reference proteome;Repressor;Stress response;Transcription;Transcription regulation;Ubl conjugation SUBCELLULAR LOCATION: Nucleus Cytoplasm Note=Shuttles between cytoplasm and nucleus. Nuclear translocation is inhibited by phosphorylation by AKT proteins. Association with ftt-2 sequesters daf-16 in the cytoplasm. Association with prmt-1 allows for translocation to the nucleus. Nuclear translocation is promoted by phosphorylation by unc-43 and inhibited by dephosphorylation by tax-6. Nuclear translocation is promoted by jnk-1 upon heat stress and by sek-1 upon oxidative stress. Nucleocytoplasmic shuttling is induced by starvation, heat treatment, hypergravity, reactive oxygen species (generated by juglone), exposure to tributyltin or 4-hydroxy-E-globularinin (4-HEG) and the flavonoids kaempferol and fisetin. Nuclear localization induced by nematophagous fungal infection.; SUBCELLULAR LOCATION: [Isoform a]: Nucleus Cytoplasm Note=Ratio of nuclear to cytoplasmic localization is daf-2-dependent.; SUBCELLULAR LOCATION: [Isoform d]: Nucleus Cytoplasm Note=Ratio of nuclear to cytoplasmic localization is daf-2-dependent.; SUBCELLULAR LOCATION: [Isoform f]: Nucleus Cytoplasm Note=Ratio of nuclear to cytoplasmic localization is daf-2-dependent. " O17514,"PROTEIN NAMES: Histone-lysine N-methyltransferase mes-2 (E(z) homolog) (Maternal-effect sterile protein 2) PROTEIN FAMILY: Class V-like SAM-binding methyltransferase superfamily, Histone-lysine methyltransferase family, EZ subfamily ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. EZ subfamily. FUNCTION: Polycomb group (PcG) protein. Catalytic subunit of a the mes-2/mes-3/mes-6 complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target genes. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development. In association with the nfya-1-NF-Y complex, may play a role in repressing the expression of the homeobox protein egl-5 in tissues such as the head. PcG proteins are not required to initiate repression, but to maintain it during later stages of development. The mes-2/mes-3/mes-6 complex may participate in the global inactivation of the X chromosomes in germline cells. This complex is required to exclude mes-4 from the inactivated X-chromosomes in germline cells. Required for small-RNA-induced H3K27 trimethylation. Involved in the negative regulation of lifespan in a germline-independent fashion. KEYWORDS: Developmental protein;Methyltransferase;Nucleus;Reference proteome;Repressor;S-adenosyl-L-methionine;Transcription;Transcription regulation;Transferase SUBCELLULAR LOCATION: Nucleus " O17645,PROTEIN NAMES: Heparan sulfate 2-O-sulfotransferase hst-2 (Heparan sulfotransferase 2) (HS2ST1 homolog) PROTEIN FAMILY: Sulfotransferase 3 family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the sulfotransferase 3 family. FUNCTION: Catalyzes the transfer of sulfate to the C2-position of selected hexuronic acid residues within the maturing heparan sulfate (HS). Involved in cell adhesion and guidance by specifically modifying proteoglycans in the extracellular matrix and on the cell surface that are essential for axon migrations. KEYWORDS: Developmental protein;Disulfide bond;Glycoprotein;Golgi apparatus;Membrane;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus membrane ; Single-pass type II membrane protein O17679,"PROTEIN NAMES: Histone-lysine N-methyltransferase set-6 PROTEIN FAMILY: Class V-like SAM-binding methyltransferase superfamily ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the class V-like SAM-binding methyltransferase superfamily. FUNCTION: Histone methyltransferase that specifically di- and trimethylates 'Lys-9' of histone H3 (H3K9me2 and H3K9me3, respectively); involved in positively modulating the rate of age-related behavioral deterioration. May repress the expression of mitochondrial function-related genes by occupying their promoter regions, working in concert with probable chromatin reader protein, baz-2. Involved in modulation of the mitochondrial unfolded protein response (UPR). Regulates level of expression of bas-1, a serotonin (5-HT) and dopamine synthesizing enzyme (DOPA decarboxylase). Negatively modulates levels of endogenous 5-HT and dopamine with aging. Involved in modulating longevity, probably as a result of enhanced stress resistance via mechanisms related to dietary restriction and mitochondrial function. KEYWORDS: Chromosome;Methyltransferase;Nucleus;Reference proteome;S-adenosyl-L-methionine;Transferase SUBCELLULAR LOCATION: Nucleus Chromosome " O17894,"PROTEIN NAMES: Homeobox protein unc-39 (Homeobox protein ceh-35) (Uncoordinated protein 39) PROTEIN FAMILY: SIX/Sine oculis homeobox family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the SIX/Sine oculis homeobox family. FUNCTION: Probable transcription factor required for differentiation and migration of neuronal cells, such as RID and CAN neurons. Specifically, plays a role in the terminal differentiation of RID peptidergic neurons. Also required for CAN neuron axon guidance. KEYWORDS: Developmental protein;DNA-binding;Homeobox;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " O18209,"PROTEIN NAMES: Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase wee-1.3 (Lethal protein 37) (Myt1 kinase) PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family, WEE1 subfamily ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily. FUNCTION: Acts as a negative regulator of entry into mitosis (G2 to M transition) by phosphorylation of the CDK1 kinase during oocyte maturation. Required for oocyte maturation, embryonic development, germline proliferation and initiation of meiosis during spermatogenesis. Required for chromosome structure during mitosis and negative regulation of nuclear envelope breakdown. KEYWORDS: ATP-binding;Cell cycle;Cytoplasm;Developmental protein;Differentiation;Golgi apparatus;Kinase;Magnesium;Membrane;Metal-binding;Nucleotide-binding;Oogenesis;Reference proteome;Serine/threonine-protein kinase;Spermatogenesis;Transferase SUBCELLULAR LOCATION: Golgi apparatus membrane ; Peripheral membrane protein Cytoplasm " O18214,"PROTEIN NAMES: Protein male abnormal 3 ORGANISM: Caenorhabditis elegans FUNCTION: Transcription factor which binds the DNA motif 5'-[CGA][TCA][TA]ACAATGT[AT][TGA]C-3', probably as a monomer. Acts partially redundantly with the transcription factor dmd-3 to coordinate tail tip cell fusion and retraction and thereby regulate male tail tip morphogenesis. Promotes male-specific development of two tissues, the peripheral nervous system and the intestine. In the peripheral nervous system, directs differentiation of sensory ray neuroblasts into peripheral sense organs. In the intestine, causes repression of vitellogenin gene transcription. KEYWORDS: Alternative splicing;Developmental protein;Differentiation;DNA-binding;Metal-binding;Nucleus;Reference proteome;Repeat;Sexual differentiation;Zinc SUBCELLULAR LOCATION: Nucleus " O18276,"PROTEIN NAMES: Gamma-aminobutyric acid receptor subunit beta (GABA(A) receptor subunit beta) PROTEIN FAMILY: Ligand-gated ion channel family, Gamma-aminobutyric acid receptor subfamily ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. FUNCTION: GABA, an inhibitory neurotransmitter, mediates neuronal inhibition by binding to the GABA receptor and opening an integral chloride channel. KEYWORDS: Cell membrane;Chloride;Chloride channel;Disulfide bond;Glycoprotein;Ion channel;Ion transport;Ligand-gated ion channel;Membrane;Postsynaptic cell membrane;Receptor;Reference proteome;Signal;Synapse;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Postsynaptic cell membrane ; Multi-pass membrane protein Cell membrane ; Multi-pass membrane protein " O18734,"PROTEIN NAMES: Protein phosphatase 1 regulatory subunit 14A (17 kDa PKC-potentiated inhibitory protein of PP1) (Protein kinase C-potentiated inhibitor protein of 17 kDa) (CPI-17) PROTEIN FAMILY: PP1 inhibitor family ORGANISM: Sus scrofa (Pig) SIMILARITY: Belongs to the PP1 inhibitor family. FUNCTION: Inhibitor of PPP1CA. Has over 1000-fold higher inhibitory activity when phosphorylated, creating a molecular switch for regulating the phosphorylation status of PPP1CA substrates and smooth muscle contraction. KEYWORDS: 3D-structure;Cytoplasm;Direct protein sequencing;Phosphoprotein;Protein phosphatase inhibitor;Reference proteome SUBCELLULAR LOCATION: Cytoplasm " O18738,"PROTEIN NAMES: Dystroglycan 1 (Dystroglycan) (Dystrophin-associated glycoprotein 1) [Cleaved into: Alpha-dystroglycan (Alpha-DG); Beta-dystroglycan (Beta-DG)] ORGANISM: Bos taurus (Bovine) FUNCTION: The dystroglycan complex is involved in a number of processes including laminin and basement membrane assembly, sarcolemmal stability, cell survival, peripheral nerve myelination, nodal structure, cell migration, and epithelial polarization.; FUNCTION: [Alpha-dystroglycan]: Extracellular peripheral glycoprotein that acts as a receptor for extracellular matrix proteins containing laminin-G domains. Receptor for laminin-2 (LAMA2) and agrin in peripheral nerve Schwann cells. Also acts as a receptor for laminin LAMA5.; FUNCTION: [Beta-dystroglycan]: Transmembrane protein that plays important roles in connecting the extracellular matrix to the cytoskeleton. Acts as a cell adhesion receptor in both muscle and non-muscle tissues. Receptor for both DMD and UTRN and, through these interactions, scaffolds axin to the cytoskeleton. Also functions in cell adhesion-mediated signaling and implicated in cell polarity (By similarity). KEYWORDS: Cell membrane;Cytoplasm;Cytoskeleton;Disulfide bond;Glycoprotein;Membrane;Nucleus;Phosphoprotein;Postsynaptic cell membrane;Reference proteome;Secreted;Signal;Synapse;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: [Alpha-dystroglycan]: Secreted, extracellular space.; SUBCELLULAR LOCATION: [Beta-dystroglycan]: Cell membrane ; Single-pass type I membrane protein. Cytoplasm, cytoskeleton. Nucleus, nucleoplasm. Cell membrane, sarcolemma Postsynaptic cell membrane Note=The monomeric form translocates to the nucleus via the action of importins and depends on RAN. Nuclear transport is inhibited by Tyr-892 phosphorylation. In skeletal muscle, this phosphorylated form locates to a vesicular internal membrane compartment. In muscle cells, sarcolemma localization requires the presence of ANK2, while localization to costameres requires the presence of ANK3. Localizes to neuromuscular junctions (NMJs) in the presence of ANK2 (By similarity). Colocalizes with ERM proteins in Schwann-cell microvilli (By similarity). In peripheral nerves, localizes to the Schwann cell membrane. " O18956,"PROTEIN NAMES: Ectonucleoside triphosphate diphosphohydrolase 1 (ATP diphosphohydrolase) (ATP-DPH) (ATPDase) (Ecto-ATP diphosphohydrolase 1) (Ecto-ATPDase 1) (Ecto-ATPase 1) (Ecto-apyrase) (Lymphoid cell activation antigen) (Nucleoside triphosphate diphosphohydrolase 1) (NTPDase1) (CD antigen CD39) PROTEIN FAMILY: GDA1/CD39 NTPase family ORGANISM: Bos taurus (Bovine) SIMILARITY: Belongs to the GDA1/CD39 NTPase family. FUNCTION: Catalyzes the hydrolysis of both di- and triphosphate nucleotides (NDPs and NTPs) and hydrolyze NTPs to nucleotide monophosphates (NMPs) in two distinct successive phosphate-releasing steps, with NDPs as intermediates and participates in the regulation of extracellular levels of nucleotides. By hydrolyzing proinflammatory ATP and platelet-activating ADP to AMP, it blocks platelet aggregation and supports blood flow. KEYWORDS: ATP-binding;Calcium;Direct protein sequencing;Disulfide bond;Glycoprotein;Hydrolase;Magnesium;Membrane;Nucleotide-binding;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Multi-pass membrane protein Membrane, caveola " O22056,"PROTEIN NAMES: RNA polymerase sigma factor sigB (Sigma factor B) (Sigma-B) (Protein ABERRANT CHLOROPLAST 1) (RNA polymerase sigma factor sig1) (Atsig1) (Sigma factor 1) (RNA polymerase sigma factor sig2) (Atsig2) (Sigma factor 2) (RNA polymerase sigma factor sigA) (Sigma factor A) (Sigma-A) PROTEIN FAMILY: Sigma-70 factor family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the sigma-70 factor family. FUNCTION: Required for the transition of plastids into chloroplasts by coordinating nuclear and chloroplastic genomes under light conditions. Sigma factors are initiation factors that promote the attachment of plastid-encoded RNA polymerase (PEP) to specific initiation sites and are then released. Promotes the biosynthesis of plastid-encoded tRNAs (e.g. trnE-UUC and trnV-UAC). KEYWORDS: Chloroplast;DNA-binding;Plastid;Reference proteome;Sigma factor;Transcription;Transcription regulation;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast " O22133,"PROTEIN NAMES: Protein BRI1-5 ENHANCED 1 PROTEIN FAMILY: NAD(P)-dependent epimerase/dehydratase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the NAD(P)-dependent epimerase/dehydratase family. FUNCTION: Element of the brassinosteroid metabolic pathway that regulates typhasterol (TY), castasterone (CS) and brassinolide (BL) levels. Involved in the control of organ elongation. PATHWAY: Plant hormone biosynthesis; brassinosteroid biosynthesis. KEYWORDS: Brassinosteroid biosynthesis;Cytoplasm;Developmental protein;Lipid biosynthesis;Lipid metabolism;NADP;Oxidoreductase;Reference proteome;Steroid biosynthesis SUBCELLULAR LOCATION: Cytoplasm MISCELLANEOUS: The ben1-1D (bri1-5 enhanced 1-1dominant) activation-tagging mutant suppresses the bri1-5 weak mutant allele of the brassinosteroid receptor gene BRI1." O22145,"PROTEIN NAMES: Probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial (Glycoprotease 1) (N6-L-threonylcarbamoyladenine synthase) (t(6)A synthase) (t(6)A37 threonylcarbamoyladenosine biosynthesis protein GCP1) (tRNA threonylcarbamoyladenosine biosynthesis protein GCP1) PROTEIN FAMILY: KAE1 / TsaD family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the KAE1 / TsaD family. FUNCTION: Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in mitochondrial tRNAs that read codons beginning with adenine. Probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. Involved in mitochondrial genome maintenance (By similarity). May have a role in embryonic development in plants. KEYWORDS: Acyltransferase;Membrane;Metal-binding;Mitochondrion;Mitochondrion inner membrane;Reference proteome;Transferase;Transit peptide;tRNA processing SUBCELLULAR LOCATION: Mitochondrion inner membrane ; Peripheral membrane protein " O22208,"PROTEIN NAMES: bZIP transcription factor 17 (AtbZIP17) PROTEIN FAMILY: BZIP family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the bZIP family. FUNCTION: Transcriptional activator involved in salt and osmotic stress responses. Functions as a stress sensor and transducer in a signaling pathway that resembles an ER stress response. Following salt stress, BZIP17 is cleaved by SBT6.1 (S1P) and S2P at the C-terminus and the N-terminal bZIP component is translocated to the nucleus, where it activates the expression of salt stress response genes. Functions as a stress sensor and transducer in ER stress signaling pathway. ER stress induces proteolysis of BZIP17 by SBT6.1 (S1P) and S2P, and the N-terminal bZIP component is translocated to the nucleus, where it activates the expression and production of ER chaperones, as well as protein involved in brassinosteroid (BR) signaling, which is required for stress acclimation and growth. KEYWORDS: Activator;DNA-binding;Endoplasmic reticulum;Glycoprotein;Golgi apparatus;Membrane;Nucleus;Reference proteome;Stress response;Transcription;Transcription regulation;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Single-pass membrane protein Golgi apparatus membrane ; Single-pass membrane protein Nucleus Note=Translocates to the nucleus following salt treatment, heat shock or tunicamycin treatment (ER stress). Relocation of BZIP17 from ER to the nucleus occurs through the Golgi and is S2P-dependent. " O22212,"PROTEIN NAMES: U4/U6 small nuclear ribonucleoprotein PRP4-like protein (Protein EMBRYO DEFECTIVE 2776) (Protein LACHESIS) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Participates in pre-mRNA splicing. Part of the U4/U5/U6 tri-snRNP complex, one of the building blocks of the spliceosome (By similarity). Essential for reproduction. In female gametophyte, is necessary for the egg cell and central cell fate determination and hence reproductive success. Involved in a mechanism that prevents accessory cells from adopting gametic cell fate. Modulates egg cell signaling center that regulates the development of all female gametophytic cells. KEYWORDS: mRNA processing;mRNA splicing;Nucleus;Reference proteome;Repeat;Spliceosome;WD repeat SUBCELLULAR LOCATION: Nucleus speckle Note=Colocalizes with CLO. " O22213,"PROTEIN NAMES: Cytokinin dehydrogenase 1 (Cytokinin oxidase 1) (AtCKX1) (CKO 1) PROTEIN FAMILY: Oxygen-dependent FAD-linked oxidoreductase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the oxygen-dependent FAD-linked oxidoreductase family. FUNCTION: Catalyzes the oxidation of cytokinins, a family of N(6)-substituted adenine derivatives that are plant hormones, where the substituent is an isopentenyl group. Catalyzes in vitro the oxidation of various types of cytokinin nucleotides that are known as direct products of cytokinin biosynthesis. Promotes adventitious root initiation downstream of MYC2-dependent jasmonate signaling. Cytokinin degraded by CKX1 is required for cell division in the female gametophyte by modulating the expression of cell cycle genes. KEYWORDS: FAD;Flavoprotein;Glycoprotein;Oxidoreductase;Reference proteome;Signal;Vacuole SUBCELLULAR LOCATION: Vacuole MISCELLANEOUS: The enzymatic activity is significantly greater under acidic conditions in vitro." O22268,"PROTEIN NAMES: Ribosomal RNA small subunit methyltransferase (18S nuclear rRNA (adenine(1785)-N(6)/adenine(1786)-N(6))-dimethyltransferase) (Adenosine dimethyl transferase 1A) (Dimethyladenosine transferase 1A) PROTEIN FAMILY: Class I-like SAM-binding methyltransferase superfamily, rRNA adenine N(6)-methyltransferase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. FUNCTION: N6-adenine methyltransferase which modifies the AA dinucleotide at the plant nuclear 18S rRNA nucleotides A1785 and A1786. Required for generating appropriate patterns of gene expression during root development, including the cell-specific expression of transcriptional regulators involved in root hair and non-hair cells patterning. KEYWORDS: Methyltransferase;Nucleus;Reference proteome;RNA-binding;rRNA processing;S-adenosyl-L-methionine;Transferase SUBCELLULAR LOCATION: Nucleus Nucleus, nucleolus MISCELLANEOUS: The inability to isolate a null mutant suggests that DIM1A is essential for viability." O22315,"PROTEIN NAMES: Serine/arginine-rich-splicing factor SR34 (At-SR34) (At-SRp34) (AtSR34) (Pre-mRNA-splicing factor SF2) (SR1 protein) PROTEIN FAMILY: Splicing factor SR family, SR subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the splicing factor SR family. SR subfamily. FUNCTION: General splicing factor. Can promote splice site selection in vitro presumably by antagonizing the effects of the A1 heterogeneous nuclear ribonucleoprotein. May have an essential function during early plant development. KEYWORDS: Alternative splicing;mRNA processing;mRNA splicing;Nucleus;Phosphoprotein;Reference proteome;Repeat;RNA-binding;Spliceosome SUBCELLULAR LOCATION: Nucleus speckle Nucleus, nucleoplasm MISCELLANEOUS: The splicing pattern of the pre-mRNA is regulated in a tissue-specific manner and by development, and changes in response to various types of abiotic stresses and hormones." O22806,"PROTEIN NAMES: Mitotic spindle checkpoint protein BUBR1 (BUB1-related protein 1) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions may be to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C (By similarity). KEYWORDS: Alternative splicing;Cell cycle;Centromere;Chromosome;Cytoplasm;Cytoskeleton;Kinetochore;Nucleus;Reference proteome SUBCELLULAR LOCATION: Chromosome Cytoplasm Nucleus Chromosome, centromere, kinetochore Cytoplasm, cytoskeleton, spindle Cytoplasm, cytoskeleton, microtubule organizing center Note=Cytoplasmic in interphase cells. Accumulates onto both kinetochores and the spindle microtubules in cell arrested in metaphase. Associates with unattached kinetochores in early prophase upon spindle assembly checkpoint (SAC) activation. Present in chromocenters. " O22893,"PROTEIN NAMES: Galactinol synthase 1 (AtGolS1) (GolS-1) PROTEIN FAMILY: Glycosyltransferase 8 family, Galactosyltransferase subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the glycosyltransferase 8 family. Galactosyltransferase subfamily. FUNCTION: Galactinol synthase involved in the biosynthesis of raffinose family oligosaccharides (RFOs) that function as osmoprotectants. Promotes plant stress tolerance such as heat, chilling, salinity and methylviologen (MV), a superoxide radical generating drug, by mediating raffinose accumulation, an osmoprotective substance. KEYWORDS: Carbohydrate metabolism;Cytoplasm;Galactose metabolism;Glycosyltransferase;Manganese;Metal-binding;Reference proteome;Transferase SUBCELLULAR LOCATION: Cytoplasm " O22975,"PROTEIN NAMES: 1-acylglycerol-3-phosphate O-acyltransferase (Lipid droplet-binding protein CGI-58 homolog) PROTEIN FAMILY: Peptidase S33 family, ABHD4/ABHD5 subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the peptidase S33 family. ABHD4/ABHD5 subfamily. FUNCTION: Lysophosphatidic acid acyltransferase which functions in phosphatidic acid biosynthesis. Is highly specific for lysophosphatidic acid and able to use different acyl-CoA donors. May regulate neutral lipid accumulation and participate in the regulation of lipid turnover in vegetative cells. Possesses additional triacylglycerol lipase and phospholipase A2 activities in vitro. Is not active as esterase or lysophospholipase. KEYWORDS: Acyltransferase;Alternative splicing;Cytoplasm;Lipid biosynthesis;Lipid metabolism;Phospholipid biosynthesis;Phospholipid metabolism;Reference proteome;Transferase SUBCELLULAR LOCATION: Cytoplasm Note=Does not associate with endogenous lipid droplets. " O23144,PROTEIN NAMES: Proton pump-interactor 1 PROTEIN FAMILY: Plant Proton pump-interactor protein family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the plant Proton pump-interactor protein family. FUNCTION: Promotes AHA1 plasma membrane ATPase activity by binding to a site different from the 14-3-3 binding site. KEYWORDS: Cell membrane;Coiled coil;Endoplasmic reticulum;Membrane;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass membrane protein Endoplasmic reticulum membrane ; Single-pass membrane protein O23299,"PROTEIN NAMES: Enoyl-CoA delta isomerase 2, peroxisomal (Delta(3),Delta(2)-enoyl CoA isomerase 2) (AtECI2) (Indole-3-butyric acid response 10) PROTEIN FAMILY: Enoyl-CoA hydratase/isomerase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the enoyl-CoA hydratase/isomerase family. FUNCTION: Able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species. Essential for the beta oxidation of unsaturated fatty acids. Involved with IBR1 and IBR3 in the peroxisomal beta-oxidation of indole-3-butyric acid (IBA) to form indole-3-acetic acid (IAA), a biologically active auxin. PATHWAY: Lipid metabolism; fatty acid beta-oxidation. KEYWORDS: Fatty acid metabolism;Isomerase;Lipid metabolism;Peroxisome;Reference proteome SUBCELLULAR LOCATION: Peroxisome " O23304,"PROTEIN NAMES: Serine/threonine-protein kinase BLUS1 (Protein BLUE LIGHT SIGNALING 1) PROTEIN FAMILY: Protein kinase superfamily, STE Ser/Thr protein kinase family, STE20 subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. FUNCTION: Ser/Thr protein kinase mediating a primary step for phototropin signaling in guard cells. Essential for stomatal opening. KEYWORDS: ATP-binding;Coiled coil;Cytoplasm;Kinase;Magnesium;Metal-binding;Nucleotide-binding;Phosphoprotein;Reference proteome;Transferase SUBCELLULAR LOCATION: Cytoplasm, cytosol " O23507,"PROTEIN NAMES: Metalloendoproteinase 1-MMP (At1-MMP) PROTEIN FAMILY: Peptidase M10A family, Matrix metalloproteinases (MMPs) subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the peptidase M10A family. Matrix metalloproteinases (MMPs) subfamily. FUNCTION: Matrix metalloproteinases (MMPs) or matrixins may play a role in the degradation and remodeling of the extracellular matrix (ECM) during development or in response to stresses. Can cleave myelin basic protein as well as fluorigenic peptide substrates, McaPLANvaDpaAR-NH(2) and McaPChaGNvaHADpa-NH(2) 4-fold more efficiently than McaPLGLDpaAR-NH(2) (QF24). Active on myelin basic protein (MBP) and, to some extent, on McaPLGLDpaAR-NH(2) (QF24) and beta-casein. KEYWORDS: Cell membrane;Glycoprotein;GPI-anchor;Hydrolase;Lipoprotein;Membrane;Metal-binding;Metalloprotease;Protease;Reference proteome;Signal;Zinc;Zymogen SUBCELLULAR LOCATION: Cell membrane ; Lipid-anchor, GPI-anchor ; Extracellular side " O23661,PROTEIN NAMES: Auxin response factor 3 (Protein ETTIN) PROTEIN FAMILY: ARF family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the ARF family. FUNCTION: Auxin response factors (ARFs) are transcriptional factors that bind specifically to the DNA sequence 5'-TGTCTC-3' found in the auxin-responsive promoter elements (AuxREs). Could act as transcriptional activator or repressor. Formation of heterodimers with Aux/IAA proteins may alter their ability to modulate early auxin response genes expression. Involved in the establishment or elaboration of tissue patterning during gynoecial development. KEYWORDS: Auxin signaling pathway;Developmental protein;DNA-binding;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus. O24006,"PROTEIN NAMES: Antimicrobial peptides (IB-AMP) [Cleaved into: Basic peptide AMP3 (IB-AMP3); Basic peptide AMP1-1 (IB-AMP1-1); Basic peptide AMP1-2 (IB-AMP1-2); Basic peptide AMP1-3 (IB-AMP1-3); Basic peptide AMP2 (IB-AMP2); Basic peptide AMP4 (IB-AMP4)] ORGANISM: Impatiens balsamina (Balsam) FUNCTION: Plays a role in the defense of the germinating seed against microorganisms, by inhibiting the growth of a range of filamentous fungi and bacteria, especially Gram-positive bacteria. Not cytotoxic for cultured human cells and are the smallest known plant-derived antimicrobial peptides. Peptide IB-AMP4 has a higher antifungal activity than IB-AMP1. KEYWORDS: Antibiotic;Antimicrobial;Cleavage on pair of basic residues;Direct protein sequencing;Disulfide bond;Fungicide;Plant defense;Pyrrolidone carboxylic acid;Repeat;Secreted;Signal SUBCELLULAR LOCATION: Secreted. " O25560,"PROTEIN NAMES: Hydrogenase/urease maturation factor HypB (Hydrogenase/urease nickel incorporation protein HypB) PROTEIN FAMILY: SIMIBI class G3E GTPase family, HypB/HupM subfamily ORGANISM: Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) SIMILARITY: Belongs to the SIMIBI class G3E GTPase family. HypB/HupM subfamily. FUNCTION: Involved in the maturation of [NiFe] hydrogenases. Required for nickel insertion into the metal center of the hydrogenase. Is also required for maturation of urease. Exhibits a low intrinsic GTPase activity, which is essential for nickel insertion. KEYWORDS: 3D-structure;GTP-binding;Hydrolase;Metal-binding;Nickel;Nucleotide-binding;Reference proteome;Zinc " O25830,"PROTEIN NAMES: Bifunctional dihydropteroate synthase/dihydropteroate reductase [Includes: Dihydropteroate reductase (DHPR) ; Dihydropteroate synthase (DHPS) (Dihydropteroate pyrophosphorylase)] PROTEIN FAMILY: DHPS family ORGANISM: Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) SIMILARITY: In the C-terminal section; belongs to the DHPS family. FUNCTION: Bifunctional enzyme that catalyzes the formation of dihydropteroate, the immediate precursor of folic acid and the reduction of dihydropteroate to tetrahydropteroate. PATHWAY: Cofactor biosynthesis; tetrahydrofolate biosynthesis; 7,8-dihydrofolate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate and 4-aminobenzoate: step 1/2. KEYWORDS: FAD;Flavoprotein;FMN;Folate biosynthesis;Magnesium;Metal-binding;Multifunctional enzyme;NAD;Oxidoreductase;Reference proteome;Transferase MISCELLANEOUS: FolP can complement an E.coli strain in which the two distinct genes encoding dihydrofolate reductases folA and folM are deleted." O26314,"PROTEIN NAMES: DNA uridine endonuclease (DNA U-endo) (DNA U-endonuclease) (AP endonuclease) (ExoMt) PROTEIN FAMILY: DNA repair enzymes AP/exoA family ORGANISM: Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum) SIMILARITY: Belongs to the DNA repair enzymes AP/exoA family. FUNCTION: Involved in DNA uracil repair. Recognizes DNA uracil residues within double-stranded DNA and initiates DNA-U repair by endonucleotic incision on the 5'-side of the 2'-d-uridine residue, irrespective of the nature of the opposing nucleotide. In addition, acts as an apurinic/apyrimidinic (AP) endonuclease hydrolyzing the DNA phosphodiester backbone immediately at the 5'-side of AP sites, and as a 3'-5' exonuclease. Strongly binds to double-stranded DNA. KEYWORDS: 3D-structure;DNA damage;DNA repair;DNA-binding;Hydrolase;Lyase;Magnesium;Metal-binding;Reference proteome MISCELLANEOUS: The insertion of the side chain of Arg-209 into the DNA helical base stack seems to be crucial for the uridine recognition." O27564,"PROTEIN NAMES: Calcium-gated potassium channel MthK ORGANISM: Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum) FUNCTION: Calcium-gated potassium channel. KEYWORDS: 3D-structure;Alternative initiation;Calcium;Cell membrane;Ion channel;Ion transport;Membrane;Metal-binding;Potassium;Potassium transport;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. MISCELLANEOUS: It is not known whether calcium is the physiological ligand.; MISCELLANEOUS: Inhibited by charybdotoxin (CTX), a protein from scorpion venom." O28608,"PROTEIN NAMES: Alanine dehydrogenase (AlaDH) PROTEIN FAMILY: Ornithine cyclodeaminase/mu-crystallin family, Archaeal alanine dehydrogenase subfamily ORGANISM: Archaeoglobus fulgidus (strain ATCC 49558 / DSM 4304 / JCM 9628 / NBRC 100126 / VC-16) SIMILARITY: Belongs to the ornithine cyclodeaminase/mu-crystallin family. Archaeal alanine dehydrogenase subfamily. FUNCTION: Catalyzes the NAD(+)-dependent oxidative deamination of L-alanine to pyruvate, and the reverse reaction, the reductive amination of pyruvate. Its physiological role is not known. Cannot use NADP(+) instead of NAD(+) as a cosubstrate. In the deamination direction, can also efficiently use L-2-aminobutyrate as substrate. In the reductive amination direction, also exhibits high activity with 2-oxobutyrate and oxaloacetate as substrate. In contrast to bacterial homologs, does not exhibit any ornithine cyclodeaminase activity. KEYWORDS: 3D-structure;Direct protein sequencing;NAD;Nucleotide-binding;Oxidoreductase;Reference proteome " O29634,PROTEIN NAMES: Ribonuclease HII (RNase HII) PROTEIN FAMILY: RNase HII family ORGANISM: Archaeoglobus fulgidus (strain ATCC 49558 / DSM 4304 / JCM 9628 / NBRC 100126 / VC-16) SIMILARITY: Belongs to the RNase HII family. FUNCTION: Endonuclease that specifically degrades the RNA of RNA-DNA hybrids. KEYWORDS: 3D-structure;Cytoplasm;Endonuclease;Hydrolase;Magnesium;Manganese;Metal-binding;Nuclease;Reference proteome SUBCELLULAR LOCATION: Cytoplasm O29976,"PROTEIN NAMES: Bifunctional IPC transferase and DIPP synthase [Includes: 1L-myo-inositol-1-phosphate cytidylyltransferase (IPCT) ; CDP-L-myo-inositol myo-inositolphosphotransferase (DIPP synthase) (Di-myo-inositol-1,3'-phosphate-1'-phosphate synthase)] PROTEIN FAMILY: MobA family; CDP-alcohol phosphatidyltransferase class-I family ORGANISM: Archaeoglobus fulgidus (strain ATCC 49558 / DSM 4304 / JCM 9628 / NBRC 100126 / VC-16) SIMILARITY: In the N-terminal section; belongs to the MobA family.; SIMILARITY: In the C-terminal section; belongs to the CDP-alcohol phosphatidyltransferase class-I family. FUNCTION: Involved in biosynthesis of di-myo-inositol phosphate (DIP), a widespread organic solute in microorganisms adapted to hot environments. Catalyzes the condensation of CTP and L-myo-inositol-1-phosphate into CDP-L-myo-inositol, as well as the biosynthesis of di-myo-inositol-1,3'-phosphate-1'-phosphate (DIPP) from CDP-L-myo-inositol and L-myo-inositol-1-phosphate. The cytidylyltransferase is absolutely specific for CTP and L-myo-inositol-1-P. The DIPP synthase uses only L-myoinositol-1-phosphate as an alcohol acceptor, but CDP-glycerol, as well as CDP-L-myo-inositol and CDP-D-myoinositol, are recognized as alcohol donors. KEYWORDS: 3D-structure;Magnesium;Membrane;Metal-binding;Multifunctional enzyme;Reference proteome;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Multi-pass membrane protein " O30916,"PROTEIN NAMES: Inositol phosphate phosphatase SopB (Effector protein SopB) PROTEIN FAMILY: Phosphatase IpgD/SopB family ORGANISM: Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) SIMILARITY: Belongs to the phosphatase IpgD/SopB family. FUNCTION: Converts phosphatidylinositol 3,4,5-trisphosphate (PtdIns 3,4,5-P3) to PtdIns 3-P and prevents the transition of PtdIns 3-P to PtdIns 3,5-P2. It is one of the known effectors injected by Salmonella into the host cell and is required for invasion and for an efficient generation and maintenance of Salmonella-containing vacuole (SVC). Alteration of the phosphoinositide composition of the plasma membrane causes membrane ruffling and actin cytoskeleton rearrangements. The persistence of PtdIns 3-P diverts the SCV from the endocytic pathway resulting in enlarged vesicles, which are essential to create a favorable environment where Salmonella can replicate and avoid immune defenses of the host cell. KEYWORDS: 3D-structure;Hydrolase;Reference proteome;Secreted;Virulence SUBCELLULAR LOCATION: Secreted Note=Secreted via the type III secretion system 1 (SPI-1 T3SS). " O31266,"PROTEIN NAMES: 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase PROTEIN FAMILY: AB hydrolase superfamily ORGANISM: Paenarthrobacter nitroguajacolicus (Arthrobacter nitroguajacolicus) SIMILARITY: Belongs to the AB hydrolase superfamily. FUNCTION: Ring-cleaving dioxygenase involved in quinaldine degradation and utilization. KEYWORDS: 3D-structure;Dioxygenase;Oxidoreductase;Plasmid " O31677,"PROTEIN NAMES: 7-carboxy-7-deazaguanine synthase (CDG synthase) (Queuosine biosynthesis protein QueE) PROTEIN FAMILY: Radical SAM superfamily, 7-carboxy-7-deazaguanine synthase family ORGANISM: Bacillus subtilis (strain 168) SIMILARITY: Belongs to the radical SAM superfamily. 7-carboxy-7-deazaguanine synthase family. FUNCTION: Catalyzes the complex heterocyclic radical-mediated conversion of 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) to 7-carboxy-7-deazaguanine (CDG), a step common to the biosynthetic pathways of all 7-deazapurine-containing compounds. PATHWAY: Purine metabolism; 7-cyano-7-deazaguanine biosynthesis. KEYWORDS: 3D-structure;4Fe-4S;Iron;Iron-sulfur;Lyase;Magnesium;Metal-binding;Queuosine biosynthesis;Reference proteome;S-adenosyl-L-methionine " O31691,"PROTEIN NAMES: PtsGHI operon antiterminator (RNA-binding antitermination protein GlcT) PROTEIN FAMILY: Transcriptional antiterminator BglG family, GlcT subfamily ORGANISM: Bacillus subtilis (strain 168) SIMILARITY: Belongs to the transcriptional antiterminator BglG family. GlcT subfamily. FUNCTION: Mediates the positive regulation of the glucose PTS operon (ptsGHI) by functioning as an antiterminator factor of transcription via its interaction with the RNA-antiterminator (RAT) sequence located upstream of the ptsG gene. The RNA-binding domain of GlcT directly binds to the RNA antiterminator (RAT) sequence and prevents transcriptional termination. GlcT binding requires two identical and nearly symmetrical triple base pairings in the RAT sequence. KEYWORDS: 3D-structure;Activator;Phosphoprotein;Reference proteome;Repeat;RNA-binding;Transcription;Transcription regulation " O31854,"PROTEIN NAMES: Cyclic di-AMP synthase CdaS (c-di-AMP synthase) (Diadenylate cyclase) (DAC) PROTEIN FAMILY: Adenylate cyclase family, DacB/CdaS subfamily ORGANISM: Bacillus subtilis (strain 168) SIMILARITY: Belongs to the adenylate cyclase family. DacB/CdaS subfamily. FUNCTION: One of 3 paralogous diadenylate cyclases (DAC) in this bacteria, catalyzing the condensation of 2 ATP molecules into cyclic di-AMP (c-di-AMP) (Probable). Upon expression in E.coli leads to c-di-AMP synthesis. Overexpression of the hyperactive mutant (L44F) in the absence of c-di-AMP phosphodiesterase GdpP leads to growth defects in log phase (long curly cell filaments) that disappear upon sporulation; spore formation is normal, showing sporulation is insensitive to the excess c-di-AMP. In B.subtilis c-di-AMP is a second messenger that mediates growth, DNA repair and cell wall homeostasis; it is toxic when present in excess. KEYWORDS: ATP-binding;Coiled coil;Nucleotide-binding;Nucleotidyltransferase;Reference proteome;Transferase " O33194,"PROTEIN NAMES: D-glycerol 3-phosphate phosphatase (D,L-glycerol 3-phosphate phosphatase) (G3P phosphatase) PROTEIN FAMILY: HAD-like hydrolase superfamily ORGANISM: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) SIMILARITY: Belongs to the HAD-like hydrolase superfamily. FUNCTION: Dephosphorylates D-glycerol 3-phosphate (sn-glycerol 1-phosphate). Is the final enzyme involved in the recycling/catabolism of glycerophospholipid polar heads. To a lesser extent, is also able to act on glycerol 2-phosphate and D-ribulose 5-phosphate, but cannot use D-glyceraldehyde 3-phosphate, dihydroxyacetone-phosphate, UMP or GMP as substrates. PATHWAY: Glycerolipid metabolism. KEYWORDS: 3D-structure;Glycerol metabolism;Hydrolase;Magnesium;Metal-binding;Reference proteome " O33289,PROTEIN NAMES: Amino-acid acetyltransferase (N-acetylglutamate synthase) (AGS) (NAGS) PROTEIN FAMILY: Acetyltransferase family ORGANISM: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) SIMILARITY: Belongs to the acetyltransferase family. FUNCTION: Catalyzes the conversion of L-glutamate to alpha-N-acetyl-L-glutamate. L-glutamine is a significantly better substrate compared to L-glutamate. PATHWAY: Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 1/4. KEYWORDS: 3D-structure;Acyltransferase;Amino-acid biosynthesis;Arginine biosynthesis;Reference proteome;Transferase MISCELLANEOUS: Was identified as a high-confidence drug target. O33336,"PROTEIN NAMES: 4'-phosphopantetheinyl transferase PptT (PPTase) PROTEIN FAMILY: P-Pant transferase superfamily ORGANISM: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) SIMILARITY: Belongs to the P-Pant transferase superfamily. FUNCTION: Transfers the 4'-phosphopantetheine moiety from coenzyme A to a Ser of acyl-carrier-protein. Involved in post-translational modification of various type-I polyketide synthases required for the formation of both mycolic acids and lipid virulence factors. Acts on Pks13, Mas, PpsA, PpsB, PpsC and PpsD. Also acts on AcpM, the meromycolate extension acyl carrier protein. In addition, is involved in the activation of the acyl carrier protein MbtL and the nonribosomal peptides synthases MbtB and MbtE, which are involved in the biosynthesis of the siderophore mycobactin.; FUNCTION: Required for the replication and survival of Mycobacterium during the acute and chronic phases of infection in mice. KEYWORDS: 3D-structure;Magnesium;Metal-binding;Reference proteome;Transferase MISCELLANEOUS: Identified as a drug target." O33434,"PROTEIN NAMES: L-cysteine S-thiosulfotransferase subunit SoxA (Cytochrome c551 subunit diheme) (Protein SoxA) (SoxAX cytochrome complex subunit A) (Sulfur oxidizing protein A) (Thiosulfate-oxidizing multienzyme system protein SoxA) (TOMES protein SoxA) PROTEIN FAMILY: SoxA family ORGANISM: Paracoccus pantotrophus (Thiosphaera pantotropha) SIMILARITY: Belongs to the SoxA family. FUNCTION: C-type diheme cytochrome, which is part of the SoxAX cytochrome complex involved in sulfur oxidation. The SoxAX complex catalyzes the formation of a heterodisulfide bond between the conserved cysteine residue on a sulfur carrier SoxYZ complex subunit SoxY and thiosulfate or other inorganic sulfur substrates. This leads to the liberation of two electrons, which may be transferred from the SoxAX complex to another cytochrome c that then channels them into the respiratory electron transport chain. Some electrons may be used for reductive CO(2) fixation. KEYWORDS: 3D-structure;Direct protein sequencing;Electron transport;Heme;Iron;Metal-binding;Periplasm;Signal;Transferase;Transport;Zinc SUBCELLULAR LOCATION: Periplasm " O33830,PROTEIN NAMES: Alpha-glucosidase (Maltase) PROTEIN FAMILY: Glycosyl hydrolase 4 family ORGANISM: Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) SIMILARITY: Belongs to the glycosyl hydrolase 4 family. FUNCTION: Alpha-glycosidase with a very broad specificity. Hydrolyzes maltose and other small maltooligosaccharides but is inactive against the polymeric substrate starch. AglA is not specific with respect to the configuration at the C-4 position of its substrates because glycosidic derivatives of D-galactose are also hydrolyzed. Does not cleave beta-glycosidic bonds. KEYWORDS: 3D-structure;Carbohydrate metabolism;Cobalt;Glycosidase;Hydrolase;Manganese;Metal-binding;NAD;Nickel;Reference proteome O33832,"PROTEIN NAMES: Fructose-1,6-bisphosphatase/inositol-1-monophosphatase (FBPase/IMPase) (Inositol-1-phosphatase) (I-1-Pase) PROTEIN FAMILY: Inositol monophosphatase superfamily, FBPase class 4 family ORGANISM: Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) SIMILARITY: Belongs to the inositol monophosphatase superfamily. FBPase class 4 family. FUNCTION: Phosphatase with broad specificity; it can dephosphorylate fructose 1,6-bisphosphate, both D and L isomers of inositol-1-phosphate (I-1-P) but displaying a 20-fold higher rate of hydrolysis of D-I-1-P than of the L isomer, 2'-AMP, pNPP, inositol-2-phosphate, beta-glycerol phosphate, and alpha-D-glucose-1-phosphate. Cannot hydrolyze glucose-6-phosphate, fructose-6-phosphate, 5'-AMP and NAD(+). May be involved in the biosynthesis of a unique osmolyte, di-myo-inositol 1,1-phosphate. KEYWORDS: 3D-structure;Carbohydrate metabolism;Hydrolase;Magnesium;Metal-binding;Reference proteome " O33855,"PROTEIN NAMES: Medium-chain acyl-CoA ligase Mig (Macrophage-induced gene) (Medium-chain fatty acid acyl-CoA synthetase) PROTEIN FAMILY: ATP-dependent AMP-binding enzyme family ORGANISM: Mycobacterium avium SIMILARITY: Belongs to the ATP-dependent AMP-binding enzyme family. FUNCTION: Catalyzes the activation of medium-chain fatty acids as acyl-coenzyme A (acyl-CoA). Shows maximal activity with saturated fatty acids of medium-chain length between C6 and C12. Has lower activity with tridecanoic acid (C13), tetradecanoic acid (C14) and with unsaturated fatty acids like oleic acid (C18:1), linolenic acid (C18:3) and arachidonic acid (C20:4). Shows weak activity with some aromatic carbon acids. Involved in the metabolism of fatty acid during mycobacterial survival in macrophages. PATHWAY: Lipid metabolism; fatty acid metabolism. KEYWORDS: ATP-binding;Cell wall;Fatty acid metabolism;Ligase;Lipid metabolism;Nucleotide-binding;Secreted;Signal SUBCELLULAR LOCATION: Secreted, cell wall " O34344,"PROTEIN NAMES: Sporulation delaying protein C (SdpC) (Cannibalism toxin SDP) (Killing factor SdpC) (Toxic peptide SdpC) [Cleaved into: Sporulation delaying protein (SDP)] ORGANISM: Bacillus subtilis (strain 168) FUNCTION: Produces a 42-residue extracellular sporulation delaying protein (SDP) that collapses the proton motive force (probably both the membrane potential and pH gradient) across the cell membrane, which leads to autolysis; may form a proton channel. Induces the lysis of other B.subtilis cells that have not entered the sporulation pathway, inducing cannibalism to provide a source of nutrients to support sporulation, and at the same time delaying commitment to the energetically expensive and irreversible onset of sporulation. Addition of SDP to liquid cultures halts growth, leads to increased cell permeability and eventually cell lysis in a significant subset of the population, although some cells survive and resume growth after a lag period. Effects of SDP are irreversible within 10 minutes. Addition of SDP to solid cultures induces killing, it is much more effective than SKF (AC O31422). Has antibiotic action against Gram-positive Firmicutes (L.acidophilus, M.megaterium, P.polymyxa, S.aureus, S.epidermidis) but not Actinobacteria M.luteus or Gram-negative P.aeruginosa or K.pneumoniae. SDP induces expression of the sdpR-sdpI operon. Its maturation is dependent on SdpA and SdpB. Also functions as a ligand, binds to SdpI triggering a signal transduction cascade that protects the cell against the toxic effects of its own SDP. KEYWORDS: Antibiotic;Antimicrobial;Bacteriocin;Direct protein sequencing;Disulfide bond;Reference proteome;Secreted;Signal;Toxin;Virulence SUBCELLULAR LOCATION: Secreted Note=Produces a secreted protein originating from this gene , secreted by the general secretory pathway. " O34431,"PROTEIN NAMES: Calcium-transporting ATPase (Calcium pump) PROTEIN FAMILY: Cation transport ATPase (P-type) family, Type IIA subfamily ORGANISM: Bacillus subtilis (strain 168) SIMILARITY: Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily. FUNCTION: This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium. KEYWORDS: ATP-binding;Calcium;Calcium transport;Cell membrane;Ion transport;Magnesium;Membrane;Metal-binding;Nucleotide-binding;Phosphoprotein;Reference proteome;Translocase;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein " O34450,"PROTEIN NAMES: N-acetylglucosamine-6-phosphate deacetylase (GlcNAc 6-P deacetylase) PROTEIN FAMILY: Metallo-dependent hydrolases superfamily, NagA family ORGANISM: Bacillus subtilis (strain 168) SIMILARITY: Belongs to the metallo-dependent hydrolases superfamily. NagA family. FUNCTION: Involved in the first committed step in the biosynthesis of amino-sugar-nucleotides. Catalyzes the hydrolysis of the N-acetyl group of N-acetylglucosamine-6-phosphate (GlcNAc-6-P) to yield glucosamine 6-phosphate and acetate. Essential for growth on N-acetylglucosamine. PATHWAY: Amino-sugar metabolism; N-acetylneuraminate degradation; D-fructose 6-phosphate from N-acetylneuraminate: step 4/5. KEYWORDS: 3D-structure;Carbohydrate metabolism;Hydrolase;Iron;Metal-binding;Reference proteome " O34676,"PROTEIN NAMES: L-lysine 2,3-aminomutase (LAM) (KAM) PROTEIN FAMILY: Radical SAM superfamily, KamA family ORGANISM: Bacillus subtilis (strain 168) SIMILARITY: Belongs to the radical SAM superfamily. KamA family. FUNCTION: Catalyzes the interconversion of L-alpha-lysine and L-beta-lysine. PATHWAY: Amino-acid degradation; L-lysine degradation via acetate pathway. KEYWORDS: 4Fe-4S;Direct protein sequencing;Iron;Iron-sulfur;Isomerase;Metal-binding;Pyridoxal phosphate;Reference proteome;S-adenosyl-L-methionine " O34680,"PROTEIN NAMES: Type II methyltransferase M2.BsuMI (M2.BsuMI) (BsuMI modification methylase subunit YdiP) (Cytosine-specific methyltransferase M2.BsuMI) PROTEIN FAMILY: Class I-like SAM-binding methyltransferase superfamily, C5-methyltransferase family ORGANISM: Bacillus subtilis (strain 168) SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family. FUNCTION: A methylase, recognizes the double-stranded sequence 5'-YTCGAR-3', methylates C-3 on both strands, and protects the DNA from cleavage by the BsuMI endonuclease. KEYWORDS: Methyltransferase;Reference proteome;Restriction system;S-adenosyl-L-methionine;Transferase " O34748,"PROTEIN NAMES: ATP-dependent DNA helicase RecQ (DNA 3'-5' helicase RecQ) PROTEIN FAMILY: Helicase family, RecQ subfamily ORGANISM: Bacillus subtilis (strain 168) SIMILARITY: Belongs to the helicase family. RecQ subfamily. FUNCTION: An ATP-dependent DNA helicase which unwinds DNA in a 3'-5' direction. Requires between 2 and 5 single-stranded nucleotides on the 3'-end to initiate unwinding, is not active on blunt-ended DNA. Can target DNA replication, repair, and recombination intermediates; is active on forked, gapped and 3'-overhang DNA as well as 5'-flaps, Kappa joints, synthetic replication forks, and Holliday junctions. Prefers ATP over dATP, is not active with other nucleotides. Only unwinds short partial duplexes in vitro; SsbA enhances its activity on longer substrates. Has ss- and dsDNA-stimulated ATPase activity. Binds forked DNA strongly, ssDNA less well and dsDNA poorly. Required for DNA repair and intramolecular recombination; probably has overlapping function with RecS. It probably acts to help generate ssDNA from dsDNA breaks. KEYWORDS: ATP-binding;Cytoplasm;DNA damage;DNA recombination;DNA repair;DNA-binding;Helicase;Hydrolase;Isomerase;Metal-binding;Nucleotide-binding;Reference proteome;SOS response;Zinc SUBCELLULAR LOCATION: Cytoplasm, nucleoid Note=Localized throughout the nucleoid in the presence or absence of DNA double-strand breaks. " O34798,"PROTEIN NAMES: Peptidoglycan-N-acetylmuramic acid deacetylase PdaC (Peptidoglycan MurNAc deacetylase) (Polysaccharide deacetylase PdaC) PROTEIN FAMILY: RsiV family; Polysaccharide deacetylase family ORGANISM: Bacillus subtilis (strain 168) SIMILARITY: In the N-terminal section; belongs to the RsiV family.; SIMILARITY: In the C-terminal section; belongs to the polysaccharide deacetylase family. FUNCTION: Catalyzes the deacetylation of N-acetylmuramic acid (MurNAc) residues in peptidoglycan, a modification that confers resistance to lysosyme. Is not able to deacetylate N-acetylglucosamine (GlcNAc) residues in peptidoglycan, but can deacylate chitin oligomers such as GlcNAc4 and GlcNAc5. Is essentially not active toward chitosan (partially deacetylated GlcNAc polymer) and has very low activity toward chitin (GlcNAc polymer). Does not deacetylate GlcNAc. KEYWORDS: 3D-structure;Cell membrane;Hydrolase;Membrane;Metal-binding;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass membrane protein MISCELLANEOUS: The product of deacetylated GlcNAc4 by PdaC is GlcNAc-GlcNAc-GlcN-GlcNAc.; MISCELLANEOUS: Derepression of pdaC in cells depleted for yycFG leads to increased resistance of the cell walls to lysozyme digestion." O34840,"PROTEIN NAMES: Ca(2+)/H(+) antiporter ChaA PROTEIN FAMILY: Ca(2+):cation antiporter (CaCA) family, Cation/proton exchanger (CAX) subfamily ORGANISM: Bacillus subtilis (strain 168) SIMILARITY: Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19) family. Cation/proton exchanger (CAX) subfamily. FUNCTION: Ca(+)/H(+) antiporter that extrudes calcium in exchange for external protons. Does not transport sodium or potassium. KEYWORDS: 3D-structure;Antiport;Calcium;Calcium transport;Cell membrane;Ion transport;Membrane;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein " O34926,"PROTEIN NAMES: Pulcherriminic acid synthase (CYP134A1) (Cyclo-L-leucyl-L-leucyl dipeptide oxidase) (Cytochrome P450 CypX) PROTEIN FAMILY: Cytochrome P450 family ORGANISM: Bacillus subtilis (strain 168) SIMILARITY: Belongs to the cytochrome P450 family. FUNCTION: Involved in the biosynthesis of pulcherrimin, a red extracellular pigment. Catalyzes the oxidation of cyclo(L-Leu-L-Leu) (cLL) to yield pulcherriminic acid which forms pulcherrimin via a nonenzymic reaction with Fe(3+). Substrates with small alkyl groups (cAA, cLG, cLP) exhibit weaker binding to CYP134A1, but substrates with larger hydrophobic side chains bind in a similar regime to cLL. KEYWORDS: 3D-structure;Heme;Iron;Metal-binding;Monooxygenase;Oxidoreductase;Reference proteome " O34928,"PROTEIN NAMES: Peptidoglycan-N-acetylmuramic acid deacetylase PdaA (Peptidoglycan MurNAc deacetylase) PROTEIN FAMILY: Polysaccharide deacetylase family ORGANISM: Bacillus subtilis (strain 168) SIMILARITY: Belongs to the polysaccharide deacetylase family. FUNCTION: Catalyzes the deacetylation of N-acetylmuramic acid (MurNAc) residues in glycan strands of peptidoglycan, leading to the formation of muramic delta-lactam residues in spore cortex, after transpeptidation of deacetylated muramic acid residues. PdaA probably carries out both deacetylation and lactam ring formation and requires the product of CwlD activity on peptidoglycan as a substrate. Is required for germination. Cannot use chitin oligomer (hexa-N-acetylchitohexaose) as a substrate. KEYWORDS: 3D-structure;Cell wall biogenesis/degradation;Hydrolase;Metal-binding;Reference proteome;Signal;Sporulation MISCELLANEOUS: CwlD and PdaA are necessary and sufficient for muramic delta-lactam production in B.subtilis spore peptidoglycan." O34939,"PROTEIN NAMES: Type II methyltransferase M1.BsuMI (M1.BsuMI) (BsuMI modification methylase subunit YdiO) (Cytosine-specific methyltransferase M1.BsuMI) PROTEIN FAMILY: Class I-like SAM-binding methyltransferase superfamily, C5-methyltransferase family ORGANISM: Bacillus subtilis (strain 168) SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family. FUNCTION: A methylase, recognizes the double-stranded sequence 5'-YTCGAR-3', methylates C-3 on both strands, and protects the DNA from cleavage by the BsuMI endonuclease. KEYWORDS: DNA-binding;Methyltransferase;Reference proteome;Restriction system;S-adenosyl-L-methionine;Transferase " O35002,PROTEIN NAMES: Carboxy-terminal processing protease CtpB (C-terminal processing protease) PROTEIN FAMILY: Peptidase S41A family ORGANISM: Bacillus subtilis (strain 168) SIMILARITY: Belongs to the peptidase S41A family. FUNCTION: Involved in the signal transduction pathway leading to the proteolytic activation of the mother cell transcription factor pro-sigma-K during sporulation. The signaling serine protease CtpB triggers pro-sigma-K processing by cleaving the pre-processed regulatory protein SpoIVFA and is necessary for the proper timing of sigma-K activation. KEYWORDS: 3D-structure;Autocatalytic cleavage;Hydrolase;Protease;Reference proteome;Serine protease;Signal;Sporulation SUBCELLULAR LOCATION: Forespore intermembrane space Note=Is expressed in both the mother cell and forespore compartments but that synthesis in the forespore is both necessary and sufficient for the proper timing of pro-sigma-K processing. O35013,"PROTEIN NAMES: Putative 8-oxo-dGTP diphosphatase YtkD (8-oxo-dGTPase) (7,8-dihydro-8-oxoguanine-triphosphatase) (dGTP pyrophosphohydrolase) PROTEIN FAMILY: Nudix hydrolase family ORGANISM: Bacillus subtilis (strain 168) SIMILARITY: Belongs to the Nudix hydrolase family. FUNCTION: Involved in the GO system responsible for removing an oxidatively damaged form of guanine (7,8-dihydro-8-oxoguanine, 8-oxo-dGTP) from DNA and the nucleotide pool. 8-oxo-dGTP is inserted opposite dA and dC residues of template DNA with almost equal efficiency thus leading to A.T to G.C transversions (By similarity). Functions, in conjunction with MutT, to protect vegetatively growing cells from DNA-damaging agents such as H(2)O(2) or t-BHP (t-butylhydroperoxide). The 2 proteins do not however protect spores. According to previous research, phosphohydrolase that catalyzes the hydrolysis of all common nucleoside triphosphates as well as of the mutagenic analog 8-oxo-dGTP. The high catalytic efficiency on dGTP is in contrast to results from previous research. According to previous research, catalyzes the hydrolysis of 8-oxo-dGTP with a specific activity 413 times higher than that exhibited against dGTP. Preferentially catalyzes the hydrolysis of 8-oxo-dGTP and 8-oxo-GTP. According to previous research, hydrolyzes nucleoside triphosphates in a stepwise fashion through the diphosphate to the monophosphate, releasing two molecules of inorganic orthophosphate. KEYWORDS: 3D-structure;Hydrolase;Magnesium;Metal-binding;Reference proteome MISCELLANEOUS: According to previous research, can complement an E.coli mutT mutant. According to previous research, cannot complement an E.coli mutT mutant." O35074,"PROTEIN NAMES: Prostacyclin synthase (Hydroperoxy icosatetraenoate dehydratase) (Prostaglandin I2 synthase) PROTEIN FAMILY: Cytochrome P450 family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the cytochrome P450 family. FUNCTION: Catalyzes the biosynthesis and metabolism of eicosanoids. Catalyzes the isomerization of prostaglandin H2 to prostacyclin (= prostaglandin I2), a potent mediator of vasodilation and inhibitor of platelet aggregation. Additionally, displays dehydratase activity, toward hydroperoxyeicosatetraenoates (HPETEs), especially toward (15S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoate (15(S)-HPETE). KEYWORDS: Endoplasmic reticulum;Fatty acid biosynthesis;Fatty acid metabolism;Heme;Iron;Isomerase;Lipid biosynthesis;Lipid metabolism;Lyase;Membrane;Metal-binding;Prostaglandin biosynthesis;Prostaglandin metabolism;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Single-pass membrane protein " O35114,PROTEIN NAMES: Lysosome membrane protein 2 (85 kDa lysosomal membrane sialoglycoprotein) (LGP85) (Lysosome membrane protein II) (LIMP II) (Scavenger receptor class B member 2) PROTEIN FAMILY: CD36 family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the CD36 family. FUNCTION: Acts as a lysosomal receptor for glucosylceramidase (GBA1) targeting. KEYWORDS: Direct protein sequencing;Disulfide bond;Glycoprotein;Lipoprotein;Lysosome;Membrane;Palmitate;Receptor;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Lysosome membrane ; Multi-pass membrane protein O35227,"PROTEIN NAMES: Disintegrin and metalloproteinase domain-containing protein 7 (ADAM 7) ORGANISM: Mus musculus (Mouse) FUNCTION: Required for normal male fertility via maintenance of epithelial cell morphology in the caput epididymis and subsequently correct epididymis lumen structure required for sperm development. Plays a role in sperm motility, flagella morphology and tyrosine phosphorylation during sperm capacitance. Plays a role in normal expression levels of HSPA5, ITM2B and ADAM2 in sperm both prior to and post-capacitation. This is a non catalytic metalloprotease-like protein (Probable). KEYWORDS: Disulfide bond;Glycoprotein;Membrane;Reference proteome;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Single-pass type I membrane protein " O35254,"PROTEIN NAMES: Golgi reassembly-stacking protein 1 (Golgi peripheral membrane protein p65) (Golgi reassembly-stacking protein of 65 kDa) (GRASP65) PROTEIN FAMILY: GORASP family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the GORASP family. FUNCTION: Key structural protein of the Golgi apparatus (By similarity). The membrane cisternae of the Golgi apparatus adhere to each other to form stacks, which are aligned side by side to form the Golgi ribbon (By similarity). Acting in concert with GORASP2/GRASP55, is required for the formation and maintenance of the Golgi ribbon, and may be dispensable for the formation of stacks (By similarity). However, other studies suggest that GORASP1 plays an important role in assembly and membrane stacking of the cisternae, and in the reassembly of Golgi stacks after breakdown during mitosis. Caspase-mediated cleavage of GORASP1 is required for fragmentation of the Golgi during apoptosis. Also mediates, via its interaction with GOLGA2/GM130, the docking of transport vesicles with the Golgi membranes (By similarity). Mediates ER stress-induced unconventional (ER/Golgi-independent) trafficking of core-glycosylated CFTR to cell membrane (By similarity). KEYWORDS: 3D-structure;Direct protein sequencing;Golgi apparatus;Lipoprotein;Membrane;Metal-binding;Myristate;Phosphoprotein;Protein transport;Reference proteome;Repeat;Transport;Zinc SUBCELLULAR LOCATION: Golgi apparatus, cis-Golgi network membrane ; Peripheral membrane protein ; Cytoplasmic side " O35305,PROTEIN NAMES: Tumor necrosis factor receptor superfamily member 11A (Osteoclast differentiation factor receptor) (ODFR) (Receptor activator of NF-KB) (CD antigen CD265) ORGANISM: Mus musculus (Mouse) FUNCTION: Receptor for TNFSF11/RANKL/TRANCE/OPGL; essential for RANKL-mediated osteoclastogenesis. Its interaction with EEIG1 promotes osteoclastogenesis via facilitating the transcription of NFATC1 and activation of PLCG2. Involved in the regulation of interactions between T-cells and dendritic cells. KEYWORDS: 3D-structure;Cell membrane;Disulfide bond;Glycoprotein;Membrane;Metal-binding;Phosphoprotein;Receptor;Reference proteome;Repeat;Signal;Sodium;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein Membrane raft O35309,"PROTEIN NAMES: N-myc-interactor (Nmi) (N-myc and STAT interactor) PROTEIN FAMILY: NMI family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the NMI family. FUNCTION: Acts as a signaling pathway regulator involved in innate immune system response. In response to interleukin 2/IL2 and interferon IFN-gamma/IFNG, interacts with signal transducer and activator of transcription/STAT which activate the transcription of downstream genes involved in a multitude of signals for development and homeostasis (By similarity). Enhances the recruitment of CBP/p300 coactivators to STAT1 and STAT5, resulting in increased STAT1- and STAT5-dependent transcription (By similarity). In response to interferon IFN-alpha, associates in a complex with transcriptional regulator IFI35 to regulate immune response; the complex formation prevents proteasome-mediated degradation of IFI35 (By similarity). In complex with IFI35, negatively regulates nuclear factor NF-kappa-B signaling by inhibiting the nuclear translocation, activation and transcription of NF-kappa-B subunit p65/RELA, resulting in the inhibition of endothelial cell proliferation, migration and re-endothelialization of injured arteries (By similarity). Negatively regulates virus-triggered type I interferon/IFN production by inducing proteosome-dependent degradation of IRF7, a transcriptional regulator of type I IFN, thereby interfering with cellular antiviral responses. Beside its role as an intracellular signaling pathway regulator, also functions extracellularly as damage-associated molecular patterns (DAMPs) to promote inflammation, when actively released by macrophage to the extracellular space during cell injury or pathogen invasion (By similarity). Macrophage-secreted NMI activates NF-kappa-B signaling in adjacent macrophages through Toll-like receptor 4/TLR4 binding and activation, thereby inducing NF-kappa-B translocation from the cytoplasm into the nucleus which promotes the release of pro-inflammatory cytokines (By similarity). KEYWORDS: Coiled coil;Cytoplasm;Immunity;Innate immunity;Isopeptide bond;Nucleus;Phosphoprotein;Reference proteome;Secreted;Ubl conjugation SUBCELLULAR LOCATION: Cytoplasm Nucleus Secreted Note=Cytoplasmic NMI localizes in punctate granular structures. Nuclear localization increased following IFN-alpha treatment. Extracelullar following secretion by macrophage. " O35393,"PROTEIN NAMES: Ephrin-B3 PROTEIN FAMILY: Ephrin family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the ephrin family. FUNCTION: Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. May play a pivotal role in forebrain function. Binds to, and induce the collapse of, commissural axons/growth cones in vitro. May play a role in constraining the orientation of longitudinally projecting axons. KEYWORDS: 3D-structure;Developmental protein;Differentiation;Disulfide bond;Glycoprotein;Membrane;Methylation;Neurogenesis;Phosphoprotein;Reference proteome;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. " O35394,"PROTEIN NAMES: Prenylated Rab acceptor protein 1 (PRA1 family protein 1) PROTEIN FAMILY: PRA1 family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the PRA1 family. FUNCTION: General Rab protein regulator required for vesicle formation from the Golgi complex. May control vesicle docking and fusion by mediating the action of Rab GTPases to the SNARE complexes. In addition it inhibits the removal of Rab GTPases from the membrane by GDI1. KEYWORDS: Cell membrane;Cytoplasm;Cytoplasmic vesicle;Golgi apparatus;Membrane;Reference proteome;Synapse;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Cytoplasm Golgi apparatus Cytoplasmic vesicle, secretory vesicle, synaptic vesicle Note=According to some authors, it is an integral membrane protein, while others showed that it is cytoplasmic and membrane-associated to Golgi and synaptic vesicles. " O35423,"PROTEIN NAMES: Alanine--glyoxylate aminotransferase (AGT) (Serine--pyruvate aminotransferase, mitochondrial) (SPT) PROTEIN FAMILY: Class-V pyridoxal-phosphate-dependent aminotransferase family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. FUNCTION: [Isoform Peroxisomal]: Catalyzes the transamination of glyoxylate to glycine and contributes to the glyoxylate detoxification.; FUNCTION: [Isoform Mitochondrial]: Catalyzes the transamination between L-serine and pyruvate and weakly contributes to gluconeogenesis from the L-serine metabolism. KEYWORDS: 3D-structure;Acetylation;Alternative initiation;Aminotransferase;Mitochondrion;Peroxisome;Pyridoxal phosphate;Reference proteome;Transferase;Transit peptide SUBCELLULAR LOCATION: [Isoform Peroxisomal]: Peroxisome.; SUBCELLULAR LOCATION: [Isoform Mitochondrial]: Mitochondrion matrix " O35453,"PROTEIN NAMES: Serine protease hepsin [Cleaved into: Serine protease hepsin non-catalytic chain; Serine protease hepsin catalytic chain] PROTEIN FAMILY: Peptidase S1 family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the peptidase S1 family. FUNCTION: Serine protease that cleaves extracellular substrates, and contributes to the proteolytic processing of growth factors, such as HGF and MST1/HGFL. Plays a role in cell growth and maintenance of cell morphology. Plays a role in the proteolytic processing of ACE2 (By similarity). Mediates the proteolytic cleavage of urinary UMOD that is required for UMOD polymerization. KEYWORDS: Alternative splicing;Cell membrane;Disulfide bond;Glycoprotein;Hydrolase;Membrane;Protease;Reference proteome;Secreted;Serine protease;Signal-anchor;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Apical cell membrane ; Single-pass type II membrane protein Cell membrane ; Single-pass type II membrane protein.; SUBCELLULAR LOCATION: [Isoform 2]: Secreted MISCELLANEOUS: [Isoform 1]: Minor isoform.; MISCELLANEOUS: [Isoform 2]: Major isoform." O35458,"PROTEIN NAMES: Vesicular inhibitory amino acid transporter (GABA and glycine transporter) (Solute carrier family 32 member 1) (Vesicular GABA transporter) (rGVAT) (rat UNC-47 homolog) (RUNC-47) PROTEIN FAMILY: Amino acid/polyamine transporter 2 family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the amino acid/polyamine transporter 2 family. FUNCTION: Antiporter that exchanges vesicular protons for cytosolic 4-aminobutanoate or to a lesser extend glycine, thus allowing their secretion from nerve terminals (By similarity). The transport is equally dependent on the chemical and electrical components of the proton gradient. May also transport beta-alanine. Acidification of GABAergic synaptic vesicles is a prerequisite for 4-aminobutanoate uptake (By similarity). KEYWORDS: Cell projection;Cytoplasmic vesicle;Membrane;Neurotransmitter transport;Nitration;Reference proteome;Synapse;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane ; Multi-pass membrane protein Presynapse Note=Presents in glycine-, GABA- or GABA- and glycine-containing boutons. " O35492,"PROTEIN NAMES: Dual specificity protein kinase CLK3 (CDC-like kinase 3) PROTEIN FAMILY: Protein kinase superfamily, CMGC Ser/Thr protein kinase family, Lammer subfamily ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. Lammer subfamily. FUNCTION: Dual specificity kinase acting on both serine/threonine and tyrosine-containing substrates. Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex. May be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing and can cause redistribution of SR proteins from speckles to a diffuse nucleoplasmic distribution. Phosphorylates SRSF1 and SRSF3. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. KEYWORDS: ATP-binding;Cytoplasm;Cytoplasmic vesicle;Kinase;Nucleotide-binding;Nucleus;Phosphoprotein;Reference proteome;Serine/threonine-protein kinase;Transferase;Tyrosine-protein kinase SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Cytoplasmic vesicle, secretory vesicle, acrosome. " O35495,"PROTEIN NAMES: Cyclin-dependent kinase 14 (Cell division protein kinase 14) (Serine/threonine-protein kinase PFTAIRE-1) PROTEIN FAMILY: Protein kinase superfamily, CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. FUNCTION: Serine/threonine-protein kinase involved in the control of the eukaryotic cell cycle, whose activity is controlled by an associated cyclin. Acts as a cell-cycle regulator of Wnt signaling pathway during G2/M phase by mediating the phosphorylation of LRP6 at 'Ser-1490', leading to the activation of the Wnt signaling pathway. Acts as a regulator of cell cycle progression and cell proliferation via its interaction with CCDN3. Phosphorylates RB1 in vitro, however the relevance of such result remains to be confirmed in vivo. May also play a role in meiosis, neuron differentiation and may indirectly act as a negative regulator of insulin-responsive glucose transport (By similarity). KEYWORDS: Alternative splicing;ATP-binding;Cell cycle;Cell division;Cell membrane;Cytoplasm;Kinase;Membrane;Nucleotide-binding;Nucleus;Phosphoprotein;Reference proteome;Serine/threonine-protein kinase;Transferase;Wnt signaling pathway SUBCELLULAR LOCATION: Cell membrane ; Peripheral membrane protein Cytoplasm. Nucleus. Note=Recruited to the cell membrane by CCNY. " O35568,"PROTEIN NAMES: EGF-containing fibulin-like extracellular matrix protein 1 (Fibulin-3) (FIBL-3) (T16 protein) PROTEIN FAMILY: Fibulin family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the fibulin family. FUNCTION: Binds EGFR, the EGF receptor, inducing EGFR autophosphorylation and the activation of downstream signaling pathways. May play a role in cell adhesion and migration. May function as a negative regulator of chondrocyte differentiation. In the olfactory epithelium, it may regulate glial cell migration, differentiation and the ability of glial cells to support neuronal neurite outgrowth. KEYWORDS: Calcium;Disulfide bond;EGF-like domain;Extracellular matrix;Glycoprotein;Growth factor;Reference proteome;Repeat;Secreted;Signal SUBCELLULAR LOCATION: Secreted, extracellular space Secreted, extracellular space, extracellular matrix Note=Localizes to the lamina propria underneath the olfactory epithelium. " O35587,"PROTEIN NAMES: Transmembrane emp24 domain-containing protein 10 (Protein TMED10) (21 kDa transmembrane-trafficking protein) (Integral membrane protein p23) (Transmembrane protein Tmp21) (p24 family protein delta-1) (p24delta1) PROTEIN FAMILY: EMP24/GP25L family ORGANISM: Mesocricetus auratus (Golden hamster) SIMILARITY: Belongs to the EMP24/GP25L family. FUNCTION: Cargo receptor involved in protein vesicular trafficking and quality control in the endoplasmic reticulum (ER) and Golgi. The p24 protein family is a group of transmembrane proteins that bind coat protein complex I/COPI and coat protein complex II/COPII involved in vesicular trafficking between the membranes. Acts at the lumenal side for incorporation of secretory cargo molecules into transport vesicles and involved in vesicle coat formation at the cytoplasmic side (By similarity). Mainly functions in the early secretory pathway and cycles between the ER, ER-Golgi intermediate compartment (ERGIC) and Golgi, mediating cargo transport through COPI and COPII-coated vesicles. In COPII vesicle-mediated anterograde transport, involved in the transport of GPI-anchored proteins by acting together with TMED2 as their cargo receptor; the function specifically implies SEC24C and SEC24D of the COPII vesicle coat and lipid raft-like microdomains of the ER (By similarity). Recognizes GPI anchors structural remodeled in the ER by the GPI inositol-deacylase/PGAP1 and the metallophosphoesterase MPPE1/PGAP5 (By similarity). In COPI vesicle-mediated retrograde transport, involved in the biogenesis of COPI vesicles and vesicle coat recruitment. Involved in trafficking of amyloid beta A4 protein and soluble APP-beta release (independent from the modulation of gamma-secretase activity) (By similarity). Involved in the KDELR2-mediated retrograde transport of the toxin A subunit (CTX-A-K63)together with COPI and the COOH terminus of KDELR2 (By similarity). On Golgi membranes, acts as a primary receptor for ARF1-GDP, a GTP-binding protein involved in COPI-vesicle formation. Increases coatomer-dependent GTPase-activating activity of ARFGAP2 which mediates the hydrolysis of ARF1-bound GTP and therefore modulates protein trafficking from the Golgi apparatus. Involved in the exocytic trafficking of G protein-coupled receptors F2LR1/PAR2 (trypsin and tryspin-like enzyme receptor), OPRM1 (opioid receptor) and P2RY4 (UTD and UDP receptor) from the Golgi to the plasma membrane, thus contributing to receptor resensitization. In addition to its cargo receptor activity, may also act as a protein channel after oligomerization, facilitating the post-translational entry of leaderless cytoplasmic cargo into the ERGIC. Involved in the translocation into ERGIC, the vesicle entry and the secretion of leaderless cargos (lacking the secretion signal sequence), including the mature form of interleukin 1/IL-1 family members, the alpha-crystallin B chain HSPB5, the carbohydrate-binding proteins galectin-1/LGALS1 and galectin-3/LGALS3, the microtubule-associated protein Tau/MAPT, and the annexin A1/ANXA1; the translocation process is dependent on cargo protein unfolding and enhanced by chaperones HSP90AB1 and HSP90B1/GRP9. Could also associates with the presenilin-dependent gamma-secretase complex in order to regulate gamma-cleavages of the amyloid beta A4 protein to yield amyloid-beta 40/Abeta40 (By similarity). KEYWORDS: Cell membrane;Cytoplasmic vesicle;Endoplasmic reticulum;ER-Golgi transport;Glycoprotein;Golgi apparatus;Membrane;Methylation;Protein transport;Reference proteome;Signal;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Single-pass type I membrane protein Endoplasmic reticulum-Golgi intermediate compartment membrane ; Single-pass type I membrane protein Golgi apparatus membrane ; Single-pass type I membrane protein Golgi apparatus, cis-Golgi network membrane ; Single-pass type I membrane protein Golgi apparatus, trans-Golgi network membrane ; Single-pass type I membrane protein Cytoplasmic vesicle, secretory vesicle membrane ; Single-pass type I membrane protein Cell membrane ; Single-pass type I membrane protein Melanosome MISCELLANEOUS: Ectopic expression of TMED10 alone does not result in its proper cis-Golgi network localization. Interaction of TMED10 with TMED2 is both necessary and sufficient for transport of the couple to the cis-Golgi network, and TMED3 and/or TMED9 contribute to facilitating the process." O35664,"PROTEIN NAMES: Interferon alpha/beta receptor 2 (IFN-R-2) (IFN-alpha/beta receptor 2) (Type I interferon receptor 2) PROTEIN FAMILY: Type II cytokine receptor family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the type II cytokine receptor family. FUNCTION: Together with IFNAR1, forms the heterodimeric receptor for type I interferons (including interferons alpha, beta, epsilon, omega and kappa). Type I interferon binding activates the JAK-STAT signaling cascade, resulting in transcriptional activation or repression of interferon-regulated genes that encode the effectors of the interferon response. Mechanistically, type I interferon-binding brings the IFNAR1 and IFNAR2 subunits into close proximity with one another, driving their associated Janus kinases (JAKs) (TYK2 bound to IFNAR1 and JAK1 bound to IFNAR2) to cross-phosphorylate one another. The activated kinases phosphorylate specific tyrosine residues on the intracellular domains of IFNAR1 and IFNAR2, forming docking sites for the STAT transcription factors (STAT1, STAT2 and STAT). STAT proteins are then phosphorylated by the JAKs, promoting their translocation into the nucleus to regulate expression of interferon-regulated genes.; FUNCTION: [Isoform 2]: May be potent inhibitors of type I IFN receptor activity.; FUNCTION: [Isoform 3]: May be potent inhibitors of type I IFN receptor activity. KEYWORDS: Alternative splicing;Cell membrane;Disulfide bond;Glycoprotein;Membrane;Phosphoprotein;Receptor;Reference proteome;Secreted;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: [Isoform 1]: Cell membrane ; Single-pass type I membrane protein.; SUBCELLULAR LOCATION: [Isoform 2]: Secreted.; SUBCELLULAR LOCATION: [Isoform 3]: Secreted " O35690,PROTEIN NAMES: Paired mesoderm homeobox protein 2B (Neuroblastoma Phox) (NBPhox) (PHOX2B homeodomain protein) (Paired-like homeobox 2B) PROTEIN FAMILY: Paired homeobox family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the paired homeobox family. KEYWORDS: Developmental protein;DNA-binding;Homeobox;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus O35716,"PROTEIN NAMES: Suppressor of cytokine signaling 1 (SOCS-1) (JAK-binding protein) (JAB) (STAT-induced STAT inhibitor 1) (SSI-1) PROTEIN FAMILY: SOCS1 family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the SOCS1 family. FUNCTION: Essential negative regulator of type I and type II interferon (IFN) signaling, as well as that of other cytokines, including IL2, IL4, IL6 and leukemia inhibitory factor (LIF). Downregulates cytokine signaling by inhibiting the JAK/STAT signaling pathway. Acts by binding to JAK proteins and to IFNGR1 and inhibiting their kinase activity. In vitro, suppresses Tec protein-tyrosine activity (By similarity). Regulates IFN-gamma (IFNG)-mediated sensory neuron survival. Probable substrate recognition component of an ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (By similarity). PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Cytoplasmic vesicle;Growth regulation;Nucleus;Reference proteome;SH2 domain;Signal transduction inhibitor;Ubl conjugation pathway SUBCELLULAR LOCATION: Nucleus Cytoplasmic vesicle Note=Detected in perinuclear cytoplasmic vesicles upon interaction with FGFR3. " O35732,PROTEIN NAMES: CASP8 and FADD-like apoptosis regulator (Caspase homolog) (CASH) (Caspase-eight-related protein) (Casper) (Caspase-like apoptosis regulatory protein) (CLARP) (Cellular FLICE-like inhibitory protein) (c-FLIP) (FADD-like antiapoptotic molecule 1) (FLAME-1) (Inhibitor of FLICE) (I-FLICE) (MACH-related inducer of toxicity) (MRIT) (Usurpin) [Cleaved into: CASP8 and FADD-like apoptosis regulator subunit p43; CASP8 and FADD-like apoptosis regulator subunit p12] PROTEIN FAMILY: Peptidase C14A family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the peptidase C14A family. FUNCTION: Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity (By similarity). KEYWORDS: Alternative splicing;Apoptosis;Reference proteome;Repeat O35738,PROTEIN NAMES: Krueppel-like factor 12 (Transcriptional repressor AP-2rep) PROTEIN FAMILY: Sp1 C2H2-type zinc-finger protein family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the Sp1 C2H2-type zinc-finger protein family. FUNCTION: Confers strong transcriptional repression to the AP-2-alpha gene. Binds to a regulatory element (A32) in the AP-2-alpha gene promoter. KEYWORDS: DNA-binding;Metal-binding;Methylation;Nucleus;Phosphoprotein;Reference proteome;Repeat;Repressor;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus. O35739,PROTEIN NAMES: Krueppel-like factor 9 (Basic transcription element-binding protein 1) (BTE-binding protein 1) (GC-box-binding protein 1) (Transcription factor BTEB1) PROTEIN FAMILY: Sp1 C2H2-type zinc-finger protein family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the Sp1 C2H2-type zinc-finger protein family. FUNCTION: Transcription factor that binds to GC box promoter elements. Selectively activates mRNA synthesis from genes containing tandem repeats of GC boxes but represses genes with a single GC box. Acts as an epidermal circadian transcription factor regulating keratinocyte proliferation. KEYWORDS: Biological rhythms;DNA-binding;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Repeat;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus O35795,"PROTEIN NAMES: Ectonucleoside triphosphate diphosphohydrolase 2 (NTPDase 2) (CD39 antigen-like 1) (Ecto-ATP diphosphohydrolase 2) (Ecto-ATPDase 2) (Ecto-ATPase 2) PROTEIN FAMILY: GDA1/CD39 NTPase family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the GDA1/CD39 NTPase family. FUNCTION: In the nervous system, could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. Hydrolyzes ADP only to a marginal extent. KEYWORDS: 3D-structure;Alternative splicing;ATP-binding;Calcium;Cell membrane;Disulfide bond;Glycoprotein;Hydrolase;Magnesium;Membrane;Nucleotide-binding;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: [Isoform 1]: Cell membrane ; Multi-pass membrane protein " O35814,PROTEIN NAMES: Stress-induced-phosphoprotein 1 (STI1) (Hsc70/Hsp90-organizing protein) (Hop) ORGANISM: Rattus norvegicus (Rat) FUNCTION: Acts as a co-chaperone for HSP90AA1 (By similarity). Mediates the association of the molecular chaperones HSPA8/HSC70 and HSP90. KEYWORDS: Acetylation;Cytoplasm;Direct protein sequencing;Isopeptide bond;Nucleus;Phosphoprotein;Reference proteome;Repeat;TPR repeat;Ubl conjugation SUBCELLULAR LOCATION: Cytoplasm Nucleus Dynein axonemal particle O35926,PROTEIN NAMES: Cyclin-dependent kinase 5 activator 2 (CDK5 activator 2) (Cyclin-dependent kinase 5 regulatory subunit 2) (p39) (p39I) PROTEIN FAMILY: Cyclin-dependent kinase 5 activator family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the cyclin-dependent kinase 5 activator family. FUNCTION: Activator of CDK5/TPKII. KEYWORDS: Cell membrane;Lipid-binding;Lipoprotein;Membrane;Myristate;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cell membrane ; Lipid-anchor ; Cytoplasmic side O35930,"PROTEIN NAMES: Platelet glycoprotein Ib alpha chain (GP-Ib alpha) (GPIb-alpha) (GPIbA) (Glycoprotein Ibalpha) (CD antigen CD42b) [Cleaved into: Glycocalicin] ORGANISM: Mus musculus (Mouse) FUNCTION: GP-Ib, a surface membrane protein of platelets, participates in the formation of platelet plugs by binding to the A1 domain of vWF, which is already bound to the subendothelium. KEYWORDS: 3D-structure;Blood coagulation;Cell adhesion;Disulfide bond;Glycoprotein;Hemostasis;Leucine-rich repeat;Membrane;Phosphoprotein;Reference proteome;Repeat;Signal;Sulfation;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. MISCELLANEOUS: Platelet activation apparently involves disruption of the macromolecular complex of GP-Ib with the platelet glycoprotein IX (GP-IX) and dissociation of GP-Ib from the actin-binding protein." O35975,"PROTEIN NAMES: T-cell ecto-ADP-ribosyltransferase 2 (ADP-ribosyltransferase C2 and C3 toxin-like 2) (ARTC2) (Mono(ADP-ribosyl)transferase 2B) (NAD(+) glycohydrolase) (T-cell NAD(P)(+)--arginine ADP-ribosyltransferase 2) (T-cell differentiation marker Rt6 homolog 2) (T-cell mono(ADP-ribosyl)transferase 2) PROTEIN FAMILY: Arg-specific ADP-ribosyltransferase family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the Arg-specific ADP-ribosyltransferase family. FUNCTION: Has both NAD(+) glycohydrolase and ADP-ribosyltransferase activity. KEYWORDS: Cell membrane;Disulfide bond;Glycoprotein;Glycosyltransferase;GPI-anchor;Hydrolase;Lipoprotein;Membrane;NAD;NADP;Nucleotidyltransferase;Reference proteome;Signal;Transferase SUBCELLULAR LOCATION: Cell membrane ; Lipid-anchor, GPI-anchor " O36019,"PROTEIN NAMES: Autophagy protein 13 (Meiotically up-regulated gene 78 protein) PROTEIN FAMILY: ATG13 family, Fungi subfamily ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the ATG13 family. Fungi subfamily. FUNCTION: Component of the atg1 kinase complex that activates the atg1 kinase in a nutritional condition dependent manner through the TOR pathway, leading to autophagy. Autophagy functions to supply nitrogen and is activated when cells cannot access exogenous nitrogen, thus ensuring that they can adapt and subsequently propagate. Finally, atg13 is also required for glycogen storage during stationary phase and has a role in meiosis and sporulation. KEYWORDS: 3D-structure;Autophagy;Cytoplasm;Meiosis;Phosphoprotein;Protein transport;Reference proteome;Sporulation;Transport SUBCELLULAR LOCATION: Cytoplasm Preautophagosomal structure " O36027,"PROTEIN NAMES: Actin-binding protein wsp1 (Wiskott-Aldrich syndrome protein homolog 1) ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) FUNCTION: Has a role in regulating actin assembly, so regulating polarized growth. KEYWORDS: Actin-binding;Cytoplasm;Cytoskeleton;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton " O42721,"PROTEIN NAMES: Cell wall mannoprotein 1 PROTEIN FAMILY: Cell wall mannoprotein 1 family ORGANISM: Talaromyces marneffei (Penicillium marneffei) SIMILARITY: Belongs to the cell wall mannoprotein 1 family. FUNCTION: Constitutive protein of the cell wall. Binds fatty acids and may thus serve as a fatty acid transporter between P.marneffei and host cells during infection. Abundant antigen target of host humoral immune response. KEYWORDS: 3D-structure;Cell wall;Lipid-binding;Secreted;Signal SUBCELLULAR LOCATION: Secreted, cell wall Note=Associated with the entire thickness of the cell walls of yeast and conidia found in mold form. Localizes on the outer layers of the hyphal cell walls. " O42860,"PROTEIN NAMES: Mitotic checkpoint protein bub3 PROTEIN FAMILY: WD repeat BUB3 family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the WD repeat BUB3 family. FUNCTION: Involved in cell cycle checkpoint enforcement. Involved in recruitment of checkpoint proteins bub1 and mad3 to the kinetochores, ensuring correct spindle checkpoint function. KEYWORDS: Cell cycle;Cell division;Centromere;Chromosome;Kinetochore;Mitosis;Nucleus;Phosphoprotein;Reference proteome;Repeat;WD repeat SUBCELLULAR LOCATION: Nucleus Chromosome, centromere, kinetochore Chromosome, centromere Note=Associates with kinetochores and centromeres during the early stages of mitosis. " O42872,"PROTEIN NAMES: Exosome complex component ski6 (Ribosomal RNA-processing protein 41) PROTEIN FAMILY: RNase PH family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the RNase PH family. FUNCTION: Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and cryptic unstable transcripts (CUTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and in RNA surveillance pathways, preventing translation of aberrant mRNAs. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. ski6 is part of the hexameric ring of RNase PH domain-containing subunits proposed to form a central channel which threads RNA substrates for degradation (By similarity). KEYWORDS: Cytoplasm;Exosome;Nucleus;Reference proteome;RNA-binding;rRNA processing SUBCELLULAR LOCATION: Cytoplasm Nucleus, nucleolus " O42916,PROTEIN NAMES: Lysophospholipid acyltransferase (LPLAT) (1-acyl-sn-glycerol-3-phosphate acyltransferase) (AGPAT) (Lysophosphatidic acid acyltransferase) (LPAAT) (Lysophosphatidylcholine acyltransferase) (LPCAT) (Lysophosphatidylethanolamine acyltransferase) (LPEAT) PROTEIN FAMILY: Membrane-bound acyltransferase family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the membrane-bound acyltransferase family. FUNCTION: Membrane-bound O-acyltransferase that mediates the incorporation of unsaturated acyl chains into the sn-2 position of phospholipids. KEYWORDS: Acyltransferase;Endoplasmic reticulum;Lipid biosynthesis;Lipid metabolism;Membrane;Microsome;Phospholipid biosynthesis;Phospholipid metabolism;Phosphoprotein;Reference proteome;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass membrane protein. Microsome membrane ; Multi-pass membrane protein O43029,"PROTEIN NAMES: L-pipecolate oxidase (L-pipecolic acid oxidase) PROTEIN FAMILY: MSOX/MTOX family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the MSOX/MTOX family. FUNCTION: Oxidizes L-pipecolate and L-proline (6,7% of the activity for L-pipecolate). KEYWORDS: Cytoplasm;FAD;Flavoprotein;Glycoprotein;Nucleus;Oxidoreductase;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted Cytoplasm Nucleus " O43060,"PROTEIN NAMES: Eukaryotic translation initiation factor 3 subunit F (eIF3f) PROTEIN FAMILY: EIF-3 subunit F family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the eIF-3 subunit F family. FUNCTION: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation. KEYWORDS: Cytoplasm;Initiation factor;Phosphoprotein;Protein biosynthesis;Reference proteome SUBCELLULAR LOCATION: Cytoplasm " O43070,"PROTEIN NAMES: DNA repair and telomere maintenance protein nbs1 PROTEIN FAMILY: Nibrin family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the Nibrin family. FUNCTION: Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The MRN complex is involved in the repair of DNA double-strand breaks (DSBs) via homologous recombination (HR), an error-free mechanism which primarily occurs during S and G2 phases. The complex (1) mediates the end resection of damaged DNA, which generates proper single-stranded DNA, a key initial steps in HR, and is (2) required for the recruitment of other repair factors and efficient activation of tel1/atm upon DNA damage. The MRN complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11, to initiate end resection, which is required for single-strand invasion and recombination (By similarity). Within the MRN complex, nbs1 acts as a protein-protein adapter, which specifically recognizes and binds phosphorylated proteins, promoting their recruitment to DNA damage sites. Recruits rad32 and rad50 components of the MRN complex to DSBs in response to DNA damage (By similarity). Promotes the recruitment of tel1/atm to the DNA damage sites, activating tel1/atm function. Mediates the recruitment of phosphorylated ctp1/CtIP to DSBs, leading to cooperation between the MRN complex and ctp1/CtIP to initiate end resection. KEYWORDS: 3D-structure;Chromosome;DNA damage;DNA repair;Nucleus;Phosphoprotein;Reference proteome;Telomere SUBCELLULAR LOCATION: Nucleus Chromosome Chromosome, telomere Note=Localizes to DNA double-strand breaks (DSBs); recruited to DNA damage sites. " O43091,"PROTEIN NAMES: Decaprenyl-diphosphate synthase subunit 1 (All-trans-decaprenyl-diphosphate synthase subunit 1) (Decaprenyl pyrophosphate synthase subunit 1) PROTEIN FAMILY: FPP/GGPP synthase family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the FPP/GGPP synthase family. FUNCTION: Supplies decaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone-10. PATHWAY: Cofactor biosynthesis; ubiquinone biosynthesis. KEYWORDS: Isoprene biosynthesis;Magnesium;Metal-binding;Reference proteome;Transferase;Ubiquinone biosynthesis " O43114,PROTEIN NAMES: Origin recognition complex subunit 5 PROTEIN FAMILY: ORC5 family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the ORC5 family. FUNCTION: Component of the origin recognition complex (ORC) that binds origins of replication. It has a role in both chromosomal replication and mating type transcriptional silencing. ORC binds to multiple sites within the ars1 origin of DNA replication in an ATP-independent manner. KEYWORDS: ATP-binding;DNA replication;Nucleotide-binding;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus. O43172,"PROTEIN NAMES: U4/U6 small nuclear ribonucleoprotein Prp4 (PRP4 homolog) (hPrp4) (U4/U6 snRNP 60 kDa protein) (WD splicing factor Prp4) ORGANISM: Homo sapiens (Human) FUNCTION: Plays a role in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex that is involved in spliceosome assembly, and as component of the precatalytic spliceosome (spliceosome B complex). KEYWORDS: 3D-structure;Acetylation;Alternative splicing;Direct protein sequencing;Disease variant;mRNA processing;mRNA splicing;Nucleus;Reference proteome;Repeat;Retinitis pigmentosa;Spliceosome;WD repeat SUBCELLULAR LOCATION: Nucleus Nucleus speckle " O43173,"PROTEIN NAMES: Alpha-N-acetylneuraminate alpha-2,8-sialyltransferase ST8SIA3 (Alpha-2,8-sialyltransferase 8C) (Alpha-2,8-sialyltransferase III) (Ganglioside GD3 synthase ST8SIA3) (ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 3) (Sia-a2,3-Gal-b1,4-Glc-NAc-R:a2,8-sialyltransferase) (hST8Sia III) (Sialyltransferase 8C) (SIAT8-C) (Sialyltransferase St8Sia III) (ST8SiaIII) PROTEIN FAMILY: Glycosyltransferase 29 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the glycosyltransferase 29 family. FUNCTION: Catalyzes the transfer of sialic acid from a CMP-linked sialic acid donor onto a terminal alpha-2,3-, alpha-2,6-, or alpha-2,8-linked sialic acid of an acceptor, such as N-linked oligosaccharides of glycoproteins and glycolipids through alpha-2,8-linkages. Forms oligosialic and polysialic acid on various sialylated N-acetyllactosamine oligosaccharides of glycoproteins, including FETUB N-glycans, a2-HS-glycoprotein (AHSG) and alpha 2,3-sialylated glycosphingolipids, such as alpha 2,3-sialylparagloboside and ganglioside GM3 and to a lesser extent NCAM1 N-glycans. However, it is much more specific to N-linked oligosaccharides of glycoproteins than glycosphingolipids (By similarity). 2,3-sialylparagloboside serves as the best acceptor substrate among the glycolipids (By similarity). alpha-Neu5Ac-(2->8)-alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->4)-6S-D-GlcNAc and monosialyl and disialyl N-acetyllactosamines are the best acceptor substrates among glycoproteins. May plays critical role in the striatum by mediating the formation of disialylated and trisialylated terminal glycotopes on N- and O-glycans of specific striatal proteins, regulating their distribution in lipid rafts, affecting their interaction with other binding partners, and subsequently modulating striatal functions (By similarity). PATHWAY: Protein modification; protein glycosylation. KEYWORDS: 3D-structure;Disulfide bond;Glycoprotein;Glycosyltransferase;Golgi apparatus;Membrane;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus membrane ; Single-pass type II membrane protein " O43193,PROTEIN NAMES: Motilin receptor (G-protein coupled receptor 38) PROTEIN FAMILY: G-protein coupled receptor 1 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the G-protein coupled receptor 1 family. FUNCTION: Receptor for motilin. KEYWORDS: 3D-structure;Alternative splicing;Cell membrane;Disulfide bond;G-protein coupled receptor;Glycoprotein;Membrane;Receptor;Reference proteome;Transducer;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. O43236,"PROTEIN NAMES: Septin-4 (Bradeion beta) (Brain protein H5) (CE5B3 beta) (Cell division control-related protein 2) (hCDCREL-2) (Peanut-like protein 2) PROTEIN FAMILY: TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily, Septin GTPase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family. FUNCTION: Filament-forming cytoskeletal GTPase (Probable). Pro-apoptotic protein involved in LGR5-positive intestinal stem cell and Paneth cell expansion in the intestines, via its interaction with XIAP (By similarity). May also play a role in the regulation of cell fate in the intestine (By similarity). Positive regulator of apoptosis involved in hematopoietic stem cell homeostasis; via its interaction with XIAP (By similarity). Negative regulator of repair and hair follicle regeneration in response to injury, due to inhibition of hair follicle stem cell proliferation, potentially via its interaction with XIAP (By similarity). Plays an important role in male fertility and sperm motility (By similarity). During spermiogenesis, essential for the establishment of the annulus (a fibrous ring structure connecting the midpiece and the principal piece of the sperm flagellum) which is a requisite for the structural and mechanical integrity of the sperm (By similarity). Involved in the migration of cortical neurons and the formation of neuron leading processes during embryonic development (By similarity). Required for dopaminergic metabolism in presynaptic autoreceptors; potentially via activity as a presynaptic scaffold protein (By similarity).; FUNCTION: [Isoform ARTS]: Required for the induction of cell death mediated by TGF-beta and possibly by other apoptotic stimuli. Induces apoptosis through binding and inhibition of XIAP resulting in significant reduction in XIAP levels, leading to caspase activation and cell death. Mediates the interaction between BCL2 and XIAP, thereby positively regulating the ubiquitination and degradation of BCL2 and promoting apoptosis. KEYWORDS: 3D-structure;Alternative splicing;Cell cycle;Cell division;Cell projection;Cilium;Coiled coil;Cytoplasm;Cytoplasmic vesicle;Differentiation;Direct protein sequencing;Flagellum;GTP-binding;Mitochondrion;Nucleotide-binding;Nucleus;Phosphoprotein;Reference proteome;Spermatogenesis;Synapse;Ubl conjugation SUBCELLULAR LOCATION: Cytoplasm Cell projection, cilium, flagellum Cytoplasmic vesicle, secretory vesicle Cell projection, axon Cell projection, dendrite Perikaryon Synapse Note=In platelets, found in areas surrounding alpha-granules. Found in the sperm annulus, a fibrous ring structure connecting the midpiece and the principal piece of the sperm flagellum. Expressed and colocalized with SLC6A3 and SNCA in axon terminals, especially at the varicosities (By similarity).; SUBCELLULAR LOCATION: [Isoform ARTS]: Mitochondrion Nucleus Note=While predominantly localized in the mitochondria under resting conditions, translocates into the nucleus after TGF-beta treatment and apoptosis induction. MISCELLANEOUS: Colocalizes with alpha-synuclein in Lewy bodies in the substantia nigra pars compacta of Parkinson disease patients. Shows reduced expression in dopaminergic nerve terminals of the striatum in sporadic Parkinson disease.; MISCELLANEOUS: [Isoform ARTS]: May be defective in GTP-binding." O43242,"PROTEIN NAMES: 26S proteasome non-ATPase regulatory subunit 3 (26S proteasome regulatory subunit RPN3) (26S proteasome regulatory subunit S3) (Proteasome subunit p58) PROTEIN FAMILY: Proteasome subunit S3 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the proteasome subunit S3 family. FUNCTION: Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. KEYWORDS: 3D-structure;Alternative splicing;Isopeptide bond;Phosphoprotein;Proteasome;Reference proteome;Ubl conjugation " O43278,"PROTEIN NAMES: Kunitz-type protease inhibitor 1 (Hepatocyte growth factor activator inhibitor type 1) (HAI-1) ORGANISM: Homo sapiens (Human) FUNCTION: Inhibitor of HGFAC. Inhibits serine protease activity of ST14/matriptase in vitro. Inhibits serine protease activity of TMPRSS13, via the BPTI/Kunitz inhibitor 1 domain. KEYWORDS: 3D-structure;Alternative splicing;Cell membrane;Cytoplasm;Direct protein sequencing;Disulfide bond;Glycoprotein;Membrane;Protease inhibitor;Reference proteome;Repeat;Secreted;Serine protease inhibitor;Signal SUBCELLULAR LOCATION: Secreted Cytoplasm Cell membrane " O43296,PROTEIN NAMES: Zinc finger protein 264 PROTEIN FAMILY: Krueppel C2H2-type zinc-finger protein family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein family. FUNCTION: May be involved in transcriptional regulation. KEYWORDS: DNA-binding;Isopeptide bond;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Repeat;Transcription;Transcription regulation;Ubl conjugation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus O43365,PROTEIN NAMES: Homeobox protein Hox-A3 (Homeobox protein Hox-1E) PROTEIN FAMILY: Antp homeobox family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the Antp homeobox family. FUNCTION: Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. KEYWORDS: Developmental protein;DNA-binding;Homeobox;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus. O43427,"PROTEIN NAMES: Acidic fibroblast growth factor intracellular-binding protein (aFGF intracellular-binding protein) (FGF-1 intracellular-binding protein) ORGANISM: Homo sapiens (Human) FUNCTION: May be involved in mitogenic function of FGF1. May mediate with IER2 FGF-signaling in the establishment of laterality in the embryo (By similarity). KEYWORDS: Acetylation;Alternative splicing;Disease variant;Membrane;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus. Endomembrane system; Peripheral membrane protein. Note=Also associated with cytoplasmic membranes, particularly of mitochondria. " O43474,"PROTEIN NAMES: Krueppel-like factor 4 (Epithelial zinc finger protein EZF) (Gut-enriched krueppel-like factor) PROTEIN FAMILY: Krueppel C2H2-type zinc-finger protein family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein family. FUNCTION: Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription. KEYWORDS: 3D-structure;Activator;Alternative splicing;Cytoplasm;DNA-binding;Isopeptide bond;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Repeat;Transcription;Transcription regulation;Ubl conjugation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Cytoplasm " O43511,"PROTEIN NAMES: Pendrin (Sodium-independent chloride/iodide transporter) (Solute carrier family 26 member 4) PROTEIN FAMILY: SLC26A/SulP transporter family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the SLC26A/SulP transporter (TC 2.A.53) family. FUNCTION: Sodium-independent transporter of chloride and iodide. Mediates electroneutral chloride-bicarbonate, chloride-iodide and chloride-formate exchange with 1:1 stoichiometry. Mediates electroneutral iodide-bicarbonate exchange (By similarity). KEYWORDS: Alternative splicing;Cell membrane;Chloride;Deafness;Disease variant;Membrane;Non-syndromic deafness;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Apical cell membrane ; Multi-pass membrane protein " O43526,"PROTEIN NAMES: Potassium voltage-gated channel subfamily KQT member 2 (KQT-like 2) (Neuroblastoma-specific potassium channel subunit alpha KvLQT2) (Voltage-gated potassium channel subunit Kv7.2) PROTEIN FAMILY: Potassium channel family, KQT subfamily, Kv7.2/KCNQ2 sub-subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the potassium channel family. KQT (TC 1.A.1.15) subfamily. Kv7.2/KCNQ2 sub-subfamily. FUNCTION: Associates with KCNQ3 to form a potassium channel with essentially identical properties to the channel underlying the native M-current, a slowly activating and deactivating potassium conductance which plays a critical role in determining the subthreshold electrical excitability of neurons as well as the responsiveness to synaptic inputs. Therefore, it is important in the regulation of neuronal excitability. KCNQ2/KCNQ3 current is blocked by linopirdine and XE991, and activated by the anticonvulsant retigabine. As the native M-channel, the potassium channel composed of KCNQ2 and KCNQ3 is also suppressed by activation of the muscarinic acetylcholine receptor CHRM1. KCNQ2-KCNQ3 channel is selectively permeable to other cations besides potassium, in decreasing order of affinity K(+) > Rb(+) > Cs(+) > Na(+). Associates with Na(+)-coupled myo-inositol symporter SLC5A3 forming a coregulatory complex that alters ion selectivity, increasing Na(+) and Cs(+) permeation relative to K(+) permeation. KEYWORDS: 3D-structure;Alternative splicing;Cell membrane;Disease variant;Epilepsy;Intellectual disability;Ion channel;Ion transport;Membrane;Phosphoprotein;Potassium;Potassium channel;Potassium transport;Reference proteome;Transmembrane;Transmembrane helix;Transport;Ubl conjugation;Voltage-gated channel SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein MISCELLANEOUS: Inclusion of isoform 6 in heteromultimers results in attenuation of potassium current. Prominent expression of isoform 6 in the developing brain may alter firing repertoires of immature neurons excitability to provide cues for proliferation rather than differentiation.; MISCELLANEOUS: [Isoform 3]: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay." O43541,"PROTEIN NAMES: Mothers against decapentaplegic homolog 6 (MAD homolog 6) (Mothers against DPP homolog 6) (SMAD family member 6) (SMAD 6) (Smad6) (hSMAD6) PROTEIN FAMILY: Dwarfin/SMAD family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the dwarfin/SMAD family. FUNCTION: Transforming growth factor-beta superfamily receptors signaling occurs through the Smad family of intracellular mediators. SMAD6 is an inhibitory Smad (i-Smad) that negatively regulates signaling downstream of type I transforming growth factor-beta. Acts as a mediator of TGF-beta and BMP anti-inflammatory activities. Suppresses IL1R-TLR signaling through its direct interaction with PEL1, preventing NF-kappa-B activation, nuclear transport and NF-kappa-B-mediated expression of pro-inflammatory genes. Blocks the BMP-SMAD1 signaling pathway by competing with SMAD4 for receptor-activated SMAD1-binding. Binds to regulatory elements in target promoter regions. KEYWORDS: Alternative splicing;Craniosynostosis;Disease variant;Isopeptide bond;Metal-binding;Methylation;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation;Ubl conjugation;Zinc SUBCELLULAR LOCATION: Nucleus " O43556,"PROTEIN NAMES: Epsilon-sarcoglycan (Epsilon-SG) PROTEIN FAMILY: Sarcoglycan alpha/epsilon family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the sarcoglycan alpha/epsilon family. FUNCTION: Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix. KEYWORDS: Alternative splicing;Cell membrane;Cell projection;Cytoplasm;Cytoskeleton;Disease variant;Dystonia;Glycoprotein;Golgi apparatus;Membrane;Reference proteome;Transmembrane;Transmembrane helix;Ubl conjugation SUBCELLULAR LOCATION: Cell membrane, sarcolemma ; Single-pass membrane protein Cytoplasm, cytoskeleton Cell projection, dendrite Golgi apparatus MISCELLANEOUS: [Isoform 2]: Brain-specific." O43566,"PROTEIN NAMES: Regulator of G-protein signaling 14 (RGS14) ORGANISM: Homo sapiens (Human) FUNCTION: Regulates G protein-coupled receptor signaling cascades. Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound form. Besides, modulates signal transduction via G protein alpha subunits by functioning as a GDP-dissociation inhibitor (GDI). Has GDI activity on G(i) alpha subunits GNAI1 and GNAI3, but not on GNAI2 and G(o)-alpha subunit GNAO1. Has GAP activity on GNAI0, GNAI2 and GNAI3. May act as a scaffold integrating G protein and Ras/Raf MAPkinase signaling pathways. Inhibits platelet-derived growth factor (PDGF)-stimulated ERK1/ERK2 phosphorylation; a process depending on its interaction with HRAS and that is reversed by G(i) alpha subunit GNAI1. Acts as a positive modulator of microtubule polymerisation and spindle organization through a G(i)-alpha-dependent mechanism. Plays a role in cell division. Required for the nerve growth factor (NGF)-mediated neurite outgrowth. Involved in stress resistance. May be involved in visual memory processing capacity and hippocampal-based learning and memory. KEYWORDS: 3D-structure;Alternative splicing;Cell cycle;Cell division;Cell membrane;Cell projection;Cytoplasm;Cytoskeleton;GTPase activation;Membrane;Microtubule;Nucleus;Phosphoprotein;Reference proteome;Repeat;Signal transduction inhibitor;Synapse SUBCELLULAR LOCATION: Nucleus Nucleus, PML body Cytoplasm Membrane Cell membrane Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Cytoplasm, cytoskeleton, spindle Cytoplasm, cytoskeleton, spindle pole Cell projection, dendrite Cell projection, dendritic spine Postsynaptic density Note=Associates with the perinuclear sheaths of microtubules (MTs) surrounding the pronuclei, prior to segregating to the anastral mitotic apparatus and subsequently the barrel-shaped cytoplasmic bridge between the nascent nuclei of the emerging 2-cell embryo. Localizes to a perinuclear compartment near the microtubule-organizing center (MTOC). Expressed in the nucleus during interphase and segregates to the centrosomes and astral MTs during mitosis. Relocalizes to the nucleus in PML nuclear bodies in response to heat stress. Colocalizes with RIC8A in CA2 hippocampal neurons. Localizes to spindle poles during metaphase. Shuttles between the nucleus and cytoplasm in a CRM1-dependent manner. Recruited from the cytosol to the plasma membrane by the inactive GDP-bound forms of G(i) alpha subunits GNAI1 and GNAI3. Recruited from the cytosol to membranes by the active GTP-bound form of HRAS. Colocalizes with G(i) alpha subunit GNAI1 and RIC8A at the plasma membrane. Colocalizes with BRAF and RAF1 in both the cytoplasm and membranes (By similarity). " O43598,"PROTEIN NAMES: 5-hydroxymethyl-dUMP N-hydrolase (2'-deoxynucleoside 5'-phosphate N-hydrolase 1) (c-Myc-responsive protein RCL) PROTEIN FAMILY: 2'-deoxynucleoside 5'-phosphate N-hydrolase 1 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the 2'-deoxynucleoside 5'-phosphate N-hydrolase 1 family. FUNCTION: Part of a nucleotide salvage pathway that eliminates epigenetically modified 5-hydroxymethyl-dCMP (hmdCMP) in a two-step process entailing deamination to cytotoxic 5-hydroxymethyl-dUMP (hmdUMP), followed by its hydrolysis into 5-hydroxymethyluracil (hmU) and 2-deoxy-D-ribose 5-phosphate (deoxyribosephosphate). Catalyzes the second step in that pathway, the hydrolysis of the N-glycosidic bond in hmdUMP, degrading this cytotoxic nucleotide to avoid its genomic integration. KEYWORDS: 3D-structure;Acetylation;Alternative splicing;Cytoplasm;Glycosidase;Hydrolase;Nucleotide metabolism;Nucleus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Nucleus " O43603,PROTEIN NAMES: Galanin receptor type 2 (GAL2-R) (GALR-2) PROTEIN FAMILY: G-protein coupled receptor 1 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the G-protein coupled receptor 1 family. FUNCTION: Receptor for the hormone galanin and GALP. Receptor for the hormone spexin-1. The activity of this receptor is mediated by G proteins that activate the phospholipase C/protein kinase C pathway (via G(q)) and that inhibit adenylyl cyclase (via G(i)). KEYWORDS: 3D-structure;Cell membrane;Disulfide bond;G-protein coupled receptor;Glycoprotein;Membrane;Receptor;Reference proteome;Transducer;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. O43639,PROTEIN NAMES: Cytoplasmic protein NCK2 (Growth factor receptor-bound protein 4) (NCK adaptor protein 2) (Nck-2) (SH2/SH3 adaptor protein NCK-beta) ORGANISM: Homo sapiens (Human) FUNCTION: Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling. KEYWORDS: 3D-structure;Acetylation;Cytoplasm;Endoplasmic reticulum;Phosphoprotein;Reference proteome;Repeat;SH2 domain;SH3 domain;Translation regulation SUBCELLULAR LOCATION: Cytoplasm Endoplasmic reticulum O43660,"PROTEIN NAMES: Pleiotropic regulator 1 PROTEIN FAMILY: WD repeat PRL1/PRL2 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the WD repeat PRL1/PRL2 family. FUNCTION: Involved in pre-mRNA splicing as component of the spliceosome. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs (Probable). KEYWORDS: 3D-structure;Acetylation;Alternative splicing;Direct protein sequencing;mRNA processing;mRNA splicing;Nucleus;Phosphoprotein;Reference proteome;Repeat;Spliceosome;WD repeat SUBCELLULAR LOCATION: Nucleus Nucleus speckle MISCELLANEOUS: [Isoform 2]: May be due to a competing donor splice site." O43663,"PROTEIN NAMES: Protein regulator of cytokinesis 1 PROTEIN FAMILY: MAP65/ASE1 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the MAP65/ASE1 family. FUNCTION: Key regulator of cytokinesis that cross-links antiparrallel microtubules at an average distance of 35 nM. Essential for controlling the spatiotemporal formation of the midzone and successful cytokinesis. Required for KIF14 localization to the central spindle and midbody. Required to recruit PLK1 to the spindle. Stimulates PLK1 phosphorylation of RACGAP1 to allow recruitment of ECT2 to the central spindle. Acts as an oncogene for promoting bladder cancer cells proliferation, apoptosis inhibition and carcinogenic progression. KEYWORDS: 3D-structure;Alternative splicing;Cell cycle;Cell division;Chromosome;Coiled coil;Cytoplasm;Cytoskeleton;Microtubule;Nucleus;Oncogene;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Nucleus Cytoplasm. Cytoplasm, cytoskeleton, spindle pole Midbody Chromosome Note=Colocalized with KIF20B in the nucleus of bladder carcinoma cells at the interphase. Colocalized with KIF20B in bladder carcinoma cells at prophase, metaphase, early anaphase, at the midzone in late anaphase and at the contractile ring in telophase. Predominantly localized to the nucleus of interphase cells. During mitosis becomes associated with the mitotic spindle poles and localizes with the cell midbody during cytokinesis. Co-localizes with PRC1 in early mitosis and at the spindle midzone from anaphase B to telophase. " O43741,"PROTEIN NAMES: 5'-AMP-activated protein kinase subunit beta-2 (AMPK subunit beta-2) PROTEIN FAMILY: 5'-AMP-activated protein kinase beta subunit family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the 5'-AMP-activated protein kinase beta subunit family. FUNCTION: Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3). KEYWORDS: 3D-structure;Alternative splicing;Fatty acid biosynthesis;Fatty acid metabolism;Lipid biosynthesis;Lipid metabolism;Phosphoprotein;Reference proteome " O43752,"PROTEIN NAMES: Syntaxin-6 PROTEIN FAMILY: Syntaxin family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the syntaxin family. FUNCTION: SNARE promoting movement of transport vesicles to target membranes. Targets endosomes to the trans-Golgi network, and may therefore function in retrograde trafficking. Together with SNARE STX12, promotes movement of vesicles from endosomes to the cell membrane, and may therefore function in the endocytic recycling pathway. KEYWORDS: 3D-structure;Acetylation;Alternative splicing;Coiled coil;Endosome;Golgi apparatus;Membrane;Phosphoprotein;Protein transport;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Golgi apparatus membrane ; Single-pass type IV membrane protein Golgi apparatus, trans-Golgi network membrane ; Single-pass type IV membrane protein Recycling endosome membrane ; Single-pass type IV membrane protein " O43808,"PROTEIN NAMES: Peroxisomal membrane protein PMP34 (34 kDa peroxisomal membrane protein) (Solute carrier family 25 member 17) PROTEIN FAMILY: Mitochondrial carrier family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the mitochondrial carrier (TC 2.A.29) family. FUNCTION: Peroxisomal transporter for multiple cofactors like coenzyme A (CoA), flavin adenine dinucleotide (FAD), flavin mononucleotide (FMN) and nucleotide adenosine monophosphate (AMP), and to a lesser extent for nicotinamide adenine dinucleotide (NAD(+)), adenosine diphosphate (ADP) and adenosine 3',5'-diphosphate (PAP). May catalyze the transport of free CoA, FAD and NAD(+) from the cytosol into the peroxisomal matrix by a counter-exchange mechanism. KEYWORDS: Antiport;Cytoplasm;Membrane;Peroxisome;Reference proteome;Repeat;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cytoplasm Peroxisome membrane ; Multi-pass membrane protein " O43822,"PROTEIN NAMES: Cilia- and flagella-associated protein 410 (C21orf-HUMF09G8.5) (Leucine-rich repeat-containing protein 76) (YF5/A2) ORGANISM: Homo sapiens (Human) FUNCTION: Plays a role in cilia formation and/or maintenance (By similarity). Plays a role in the regulation of cell morphology and cytoskeletal organization. Involved in DNA damage repair. KEYWORDS: 3D-structure;Alternative splicing;Cell projection;Ciliopathy;Cilium biogenesis/degradation;Cytoplasm;Cytoskeleton;Disease variant;DNA damage;Dwarfism;Leucine-rich repeat;Mitochondrion;Phosphoprotein;Reference proteome;Repeat SUBCELLULAR LOCATION: Mitochondrion Cytoplasm, cytoskeleton, cilium basal body Cell projection, cilium, photoreceptor outer segment Cytoplasm Note=Colocalizes with NEK1 and SPATA7 at the basal body. " O43825,"PROTEIN NAMES: Beta-1,3-galactosyltransferase 2 (Beta-1,3-GalTase 2) (Beta3Gal-T2) (Beta3GalT2) (UDP-galactose:2-acetamido-2-deoxy-D-glucose 3beta-galactosyltransferase 2) PROTEIN FAMILY: Glycosyltransferase 31 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the glycosyltransferase 31 family. FUNCTION: Beta-1,3-galactosyltransferase that transfers galactose from UDP-galactose to substrates with a terminal beta-N-acetylglucosamine (beta-GlcNAc) residue. Can also utilize substrates with a terminal galactose residue, albeit with lower efficiency. Involved in the biosynthesis of the carbohydrate moieties of glycolipids and glycoproteins. Inactive towards substrates with terminal alpha-N-acetylglucosamine (alpha-GlcNAc) or alpha-N-acetylgalactosamine (alpha-GalNAc) residues. PATHWAY: Protein modification; protein glycosylation. KEYWORDS: Glycoprotein;Glycosyltransferase;Golgi apparatus;Lipid metabolism;Manganese;Membrane;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus membrane ; Single-pass type II membrane protein " O43827,"PROTEIN NAMES: Angiopoietin-related protein 7 (Angiopoietin-like factor) (Angiopoietin-like protein 7) (Cornea-derived transcript 6 protein) ORGANISM: Homo sapiens (Human) FUNCTION: Has a role in the formation and organization of the extracellular matrix. In the eye, it functions as a mediator of dexamethasone-induced matrix deposition in the trabecular meshwork, the tissue responsible for the outflow of the ocular aqueous humor and for the maintenance of intraocular pressure. Is a negative regulator of angiogenesis in the cornea, and plays a major role in maintaining corneal avascularity and transparency. KEYWORDS: Coiled coil;Direct protein sequencing;Disulfide bond;Glycoprotein;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted " O43829,"PROTEIN NAMES: Zinc finger and BTB domain-containing protein 14 (Zinc finger protein 161 homolog) (Zfp-161) (Zinc finger protein 478) (Zinc finger protein 5 homolog) (ZF5) (Zfp-5) (hZF5) PROTEIN FAMILY: Krueppel C2H2-type zinc-finger protein family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein family. FUNCTION: Transcriptional activator of the dopamine transporter (DAT), binding it's promoter at the consensus sequence 5'-CCTGCACAGTTCACGGA-3'. Binds to 5'-d(GCC)(n)-3' trinucleotide repeats in promoter regions and acts as a repressor of the FMR1 gene. Transcriptional repressor of MYC and thymidine kinase promoters. KEYWORDS: Activator;DNA-binding;Isopeptide bond;Metal-binding;Nucleus;Reference proteome;Repeat;Repressor;Transcription;Transcription regulation;Ubl conjugation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Note=Colocalizes with ZBTB21 in nucleus in HEK293 cells. " O43913,PROTEIN NAMES: Origin recognition complex subunit 5 PROTEIN FAMILY: ORC5 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the ORC5 family. FUNCTION: Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The specific DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication. KEYWORDS: 3D-structure;Alternative splicing;ATP-binding;Chromosome;DNA replication;Nucleotide-binding;Nucleus;Reference proteome;Ubl conjugation SUBCELLULAR LOCATION: Nucleus Chromosome MISCELLANEOUS: [Isoform 2]: Does not interact with ORC2. O43929,"PROTEIN NAMES: Origin recognition complex subunit 4 PROTEIN FAMILY: ORC4 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the ORC4 family. FUNCTION: Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The specific DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication. Binds histone H3 and H4 trimethylation marks H3K9me3, H3K27me3 and H4K20me3. KEYWORDS: 3D-structure;Alternative splicing;ATP-binding;Disease variant;DNA replication;DNA-binding;Dwarfism;Methylation;Nucleotide-binding;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus. " O44326,"PROTEIN NAMES: Beta-catenin-like protein hmp-2 (Protein humpback-2) PROTEIN FAMILY: Beta-catenin family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the beta-catenin family. FUNCTION: Required for cell migration during body enclosure and cell shape changes during body elongation. Plays a role in recruitment of the cadherin protein hmr-1 to adherens junctions. KEYWORDS: 3D-structure;Cell adhesion;Cell junction;Developmental protein;Reference proteome;Repeat SUBCELLULAR LOCATION: Cell junction, adherens junction " O44342,PROTEIN NAMES: Protein windbeutel (Erp29 homolog) ORGANISM: Drosophila melanogaster (Fruit fly) FUNCTION: Probable chaperone protein involved in dorsoventral axis patterning in early embryos. Probably acts by folding and targeting pipe (pip) into the Golgi. KEYWORDS: 3D-structure;Chaperone;Developmental protein;Endoplasmic reticulum;Reference proteome;Signal SUBCELLULAR LOCATION: Endoplasmic reticulum lumen MISCELLANEOUS: 'Windbeutel' means 'profiteroles' in German. O44476,"PROTEIN NAMES: Zinc phosphodiesterase ELAC protein 2 homolog (Homolog of ELAC2 protein) (tRNA 3 endonuclease 2) (tRNase Z 2) PROTEIN FAMILY: RNase Z family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the RNase Z family. FUNCTION: Zinc phosphodiesterase, which displays some tRNA 3'-processing endonuclease activity. Probably involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA (By similarity). Involved in germline proliferation. May be required for both mitosis and meiosis in germ cells.; FUNCTION: [Isoform a]: Does not regulate the mitochondrial unfolded protein response following mitochondrial stress.; FUNCTION: [Isoform b]: Plays a role in mitochondrial unfolded protein response. Upon mitochondrial stress is exported from the nucleus where its tRNA endonuclease activity is negatively regulated. In response to mitochondrial stress, might be involved in activating a transcriptional response in an ATFS-1- and DVE-1-dependent manner. May play a role in negatively regulating the mitochondrial membrane potential. KEYWORDS: Alternative splicing;Endonuclease;Hydrolase;Metal-binding;Mitochondrion;Nuclease;Nucleus;Reference proteome;Transit peptide;tRNA processing;Zinc SUBCELLULAR LOCATION: [Isoform a]: Mitochondrion.; SUBCELLULAR LOCATION: [Isoform b]: Nucleus Note=Accumulates in the nucleus upon mitochondrial stress. " O44548,"PROTEIN NAMES: Kinetochore null protein 2 PROTEIN FAMILY: KNL2 family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the KNL2 family. FUNCTION: Required for the recruitment of hcp-3, hcp-4, knl-1, bub-1 and lin-53 to kinetochores, kinetochore assembly, chromosome condensation and chromosome segregation in meiosis and mitosis. KEYWORDS: 3D-structure;Cell cycle;Cell division;Centromere;Chromosome;Chromosome partition;Coiled coil;Kinetochore;Meiosis;Mitosis;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus Chromosome, centromere Chromosome, centromere, kinetochore Note=Requires hcp-3 for chromatin localization. " O44712,PROTEIN NAMES: Aryl hydrocarbon receptor protein 1 ORGANISM: Caenorhabditis elegans FUNCTION: Probable ligand-activated transcriptional activator. Acts as a transcriptional regulator in GABAergic motor neuron cell fate specification and development. Promotes cell-type-specific expression of guanylate cyclase genes that have key roles in aggregation behavior and hyperoxia avoidance. Has no role in carbon dioxide avoidance. KEYWORDS: Activator;Alternative splicing;Behavior;DNA-binding;Neurogenesis;Nucleus;Receptor;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus O44740,"PROTEIN NAMES: Meiotic spindle formation protein 2 ORGANISM: Caenorhabditis elegans FUNCTION: Forms a heterodimeric complex in conjunction with mei-1 which severs microtubules in vitro in an ATP-dependent manner. This activity may promote rapid reorganization of cellular microtubule arrays. May act to target mei-1 within the cell. Required specifically for meiotic spindle formation in the female germline. KEYWORDS: Cell cycle;Cell division;Cytoplasm;Cytoskeleton;Meiosis;Microtubule;Reference proteome SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, spindle pole Note=Localized to the spindle poles and condensed chromatin during female meiosis. Also localized to the polar body. Correct localization requires mei-1. " O44959,"PROTEIN NAMES: Serine/threonine-protein kinase RIO1 PROTEIN FAMILY: Protein kinase superfamily, RIO-type Ser/Thr kinase family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the protein kinase superfamily. RIO-type Ser/Thr kinase family. FUNCTION: Involved in the final steps of cytoplasmic maturation of the 40S ribosomal subunit (By similarity). Despite the protein kinase domain is proposed to act predominantly as an ATPase (By similarity). The catalytic activity regulates its dynamic association with the 40S subunit (By similarity). Plays a role in oogenesis by regulating germ cell proliferation, progression through diplotene and diakinesis stages and oocyte maturation. Regulates germline development probably by regulating the phosphorylation of mpk-1. Involved in larval development. KEYWORDS: ATP-binding;Cytoplasm;Hydrolase;Kinase;Magnesium;Meiosis;Metal-binding;Nucleotide-binding;Reference proteome;Ribosome biogenesis;Serine/threonine-protein kinase;Transferase SUBCELLULAR LOCATION: Cytoplasm " O45293,"PROTEIN NAMES: Probable N-acetylgalactosaminyltransferase 8 (Protein-UDP acetylgalactosaminyltransferase 8) (UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 8) (pp-GaNTase 8) PROTEIN FAMILY: Glycosyltransferase 2 family, GalNAc-T subfamily ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily. FUNCTION: Potential glycopeptide transferase involved in O-linked oligosaccharide biosynthesis (By similarity). In contrast to other members of the family, it does not act as a peptide transferase that transfers GalNAc onto serine or threonine residue on peptides that have been tested. Some peptide transferase activity is however not excluded, considering that its appropriate peptide substrate may remain unidentified. PATHWAY: Protein modification; protein glycosylation. KEYWORDS: Disulfide bond;Glycoprotein;Glycosyltransferase;Golgi apparatus;Manganese;Membrane;Metal-binding;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus membrane ; Single-pass type II membrane protein " O45405,"PROTEIN NAMES: Chloride intracellular channel exl-1 (Exc-4-like protein) PROTEIN FAMILY: Chloride channel CLIC family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the chloride channel CLIC family. FUNCTION: Probable chloride channel. KEYWORDS: Chloride;Chloride channel;Cytoplasm;Golgi apparatus;Ion channel;Ion transport;Lysosome;Membrane;Reference proteome;Transport;Voltage-gated channel SUBCELLULAR LOCATION: Cytoplasm Membrane Lysosome membrane Golgi apparatus membrane Note=Can be translocated to membranes, possibly as integral membrane proteins, with chloride channel activity (By similarity). Localizes to lysosomal membranes in the intestine, and to Golgi apparatus in neurons and muscle. It also localizes to dense bodies, a transmembrane structure which links the muscle cytoskeleton to the neighboring hypodermis. " O45947,"PROTEIN NAMES: Putative polypeptide N-acetylgalactosaminyltransferase 10 (pp-GaNTase 10) (Protein-UDP acetylgalactosaminyltransferase 10) (UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 10) PROTEIN FAMILY: Glycosyltransferase 2 family, GalNAc-T subfamily ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily. FUNCTION: May catalyze the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. PATHWAY: Protein modification; protein glycosylation. KEYWORDS: Alternative splicing;Disulfide bond;Glycoprotein;Glycosyltransferase;Golgi apparatus;Lectin;Manganese;Membrane;Metal-binding;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus membrane ; Single-pass type II membrane protein " O45962,"PROTEIN NAMES: Regulation of longevity by E3 ubiquitin-protein ligase (RING-type E3 ubiquitin transferase rle-1) ORGANISM: Caenorhabditis elegans FUNCTION: E3 ubiquitin-protein ligase. Regulates the activity of daf-16 and is thereby involved in regulating aging and stress resistance. Regulates nsy-1 activity and thereby attenuates the activation of sek-1 and pmk-1, two components of the p38 pathway, which results in susceptibility to pathogenic bacterial infection. PATHWAY: Protein degradation; proteasomal ubiquitin-dependent pathway. KEYWORDS: Alternative splicing;Coiled coil;Metal-binding;Reference proteome;Stress response;Transferase;Ubl conjugation pathway;Zinc;Zinc-finger " O46043,PROTEIN NAMES: Poly(ADP-ribose) glycohydrolase PROTEIN FAMILY: Poly(ADP-ribose) glycohydrolase family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the poly(ADP-ribose) glycohydrolase family. FUNCTION: Poly(ADP-ribose) synthesized after DNA damage is only present transiently and is rapidly degraded by poly(ADP-ribose) glycohydrolase. Poly(ADP-ribose) metabolism is required for maintenance of the normal function of neuronal cells. KEYWORDS: 3D-structure;Hydrolase;Phosphoprotein;Reference proteome O46106,PROTEIN NAMES: Splicing factor 3A subunit 3 (Protein noisette) PROTEIN FAMILY: SF3A3 family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the SF3A3 family. FUNCTION: Probable subunit of a splicing factor complex required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA (By similarity). Involved in male fertility. KEYWORDS: Metal-binding;mRNA processing;mRNA splicing;Nucleus;Phosphoprotein;Reference proteome;Spliceosome;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus. Note=Excluded from the nucleoli. O46560,"PROTEIN NAMES: Pyridoxal kinase (Pyridoxine kinase) PROTEIN FAMILY: Pyridoxine kinase family ORGANISM: Sus scrofa (Pig) SIMILARITY: Belongs to the pyridoxine kinase family. FUNCTION: Catalyzes the phosphorylation of the dietary vitamin B6 vitamers pyridoxal (PL), pyridoxine (PN) and pyridoxamine (PM) to form pyridoxal 5'-phosphate (PLP), pyridoxine 5'-phosphate (PNP) and pyridoxamine 5'-phosphate (PMP), respectively (Probable). PLP is the active form of vitamin B6, and acts as a cofactor for over 140 different enzymatic reactions (By similarity). PATHWAY: Cofactor metabolism; pyridoxal 5'-phosphate salvage; pyridoxal 5'-phosphate from pyridoxal: step 1/1.; PATHWAY: Cofactor metabolism; pyridoxal 5'-phosphate salvage; pyridoxine 5'-phosphate from pyridoxine: step 1/1.; PATHWAY: Cofactor metabolism; pyridoxal 5'-phosphate salvage; pyridoxamine 5'-phosphate from pyridoxamine: step 1/1. KEYWORDS: Acetylation;ATP-binding;Cytoplasm;Direct protein sequencing;Kinase;Magnesium;Metal-binding;Nucleotide-binding;Phosphoprotein;Reference proteome;Sodium;Transferase;Zinc SUBCELLULAR LOCATION: Cytoplasm, cytosol " O46598,"PROTEIN NAMES: Hepatitis A virus cellular receptor 1 (HAVcr-1) (T-cell immunoglobulin and mucin domain-containing protein 1) (TIMD-1) (T-cell immunoglobulin mucin receptor 1) (TIM-1) (T-cell membrane protein 1) (CD antigen CD365) PROTEIN FAMILY: Immunoglobulin superfamily, TIM family ORGANISM: Chlorocebus aethiops (Green monkey) (Cercopithecus aethiops) SIMILARITY: Belongs to the immunoglobulin superfamily. TIM family. FUNCTION: Phosphatidylserine receptor that plays an important functional role in regulatory B-cells homeostasis including generation, expansion and suppressor functions (By similarity). As P-selectin/SELPLG ligand, plays a specialized role in activated but not naive T-cell trafficking during inflammatory responses. Controls thereby T-cell accumulation in the inflamed central nervous system (CNS) and the induction of autoimmune disease (By similarity). Regulates also expression of various anti-inflammatory cytokines and co-inhibitory ligands including IL10. Acts as a regulator of T-cell proliferation (By similarity). May play a role in kidney injury and repair (By similarity). KEYWORDS: Alternative splicing;Cell membrane;Disulfide bond;Glycoprotein;Host cell receptor for virus entry;Host-virus interaction;Immunoglobulin domain;Membrane;Receptor;Repeat;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein " O46629,"PROTEIN NAMES: Trifunctional enzyme subunit beta, mitochondrial (TP-beta) [Includes: 3-ketoacyl-CoA thiolase (Acetyl-CoA acyltransferase) (Beta-ketothiolase)] PROTEIN FAMILY: Thiolase-like superfamily, Thiolase family ORGANISM: Bos taurus (Bovine) SIMILARITY: Belongs to the thiolase-like superfamily. Thiolase family. FUNCTION: Mitochondrial trifunctional enzyme catalyzes the last three of the four reactions of the mitochondrial beta-oxidation pathway. The mitochondrial beta-oxidation pathway is the major energy-producing process in tissues and is performed through four consecutive reactions breaking down fatty acids into acetyl-CoA. Among the enzymes involved in this pathway, the trifunctional enzyme exhibits specificity for long-chain fatty acids. Mitochondrial trifunctional enzyme is a heterotetrameric complex composed of two proteins, the trifunctional enzyme subunit alpha/HADHA carries the 2,3-enoyl-CoA hydratase and the 3-hydroxyacyl-CoA dehydrogenase activities, while the trifunctional enzyme subunit beta/HADHB described here bears the 3-ketoacyl-CoA thiolase activity. PATHWAY: Lipid metabolism; fatty acid beta-oxidation. KEYWORDS: Acetylation;Acyltransferase;Endoplasmic reticulum;Fatty acid metabolism;Lipid metabolism;Membrane;Mitochondrion;Mitochondrion inner membrane;Mitochondrion outer membrane;Reference proteome;Transferase;Transit peptide SUBCELLULAR LOCATION: Mitochondrion Mitochondrion inner membrane Mitochondrion outer membrane Endoplasmic reticulum Note=Protein stability and association with membranes require HADHA. " O48528,"PROTEIN NAMES: Outer envelope pore protein 16-3, chloroplastic/mitochondrial (Chloroplastic outer envelope pore protein of 16 kDa 3) (AtOEP16-3) (OEP16-3) (Mitochondrial complex I subunit B14.7) PROTEIN FAMILY: Tim17/Tim22/Tim23 family, Plastid outer envelope porin OEP16 subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the Tim17/Tim22/Tim23 family. Plastid outer envelope porin OEP16 (TC 1.B.30) subfamily. FUNCTION: Voltage-dependent high-conductance channel with a slight cation-selectivity; selective for amino acids but excludes triosephosphates or uncharged sugars. Non-essential amino acid-selective channel protein and translocation pore for NADPH:protochlorophyllide oxidoreductase A (PORA) and possibly PORB (By similarity). KEYWORDS: 3D-structure;Acetylation;Alternative splicing;Chloroplast;Ion transport;Membrane;Mitochondrion;Mitochondrion inner membrane;Mitochondrion outer membrane;Plastid;Plastid outer membrane;Porin;Reference proteome;Transmembrane;Transmembrane beta strand;Transmembrane helix;Transport SUBCELLULAR LOCATION: Plastid, chloroplast outer membrane ; Multi-pass membrane protein. Mitochondrion outer membrane ; Multi-pass membrane protein Mitochondrion inner membrane ; Multi-pass membrane protein " O48676,"PROTEIN NAMES: UDP-glycosyltransferase 74B1 (N-hydroxythioamide S-beta-glucosyltransferase) (Thiohydroximate S-glucosyltransferase) PROTEIN FAMILY: UDP-glycosyltransferase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the UDP-glycosyltransferase family. FUNCTION: Involved in the biosynthesis of glucosinolate. In in vitro assay, may use phenylacetothiohydroximate (PATH), but not phenylacetic acid (PAA), indole-3-acetic acid (IAA) or salicylic acid (SA) as substrate. Specific for the thiohydroximate functional group and does not glucosylate the carboxylate group or a hydroxyl group. KEYWORDS: Alternative splicing;Glycosyltransferase;Reference proteome;Transferase " O48791,"PROTEIN NAMES: Stomatal closure-related actin-binding protein 1 PROTEIN FAMILY: SCAB family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the SCAB family. FUNCTION: Plant-specific actin binding protein that bundles and stabilizes microfilaments (MFs). Has no nucleation or capping activity. Regulates MF reorganization during stomatal closure. The binding to F-actin is insensitive to Ca(2+) and pH. Binds weakly to inositol phosphates. KEYWORDS: 3D-structure;Actin-binding;Coiled coil;Cytoplasm;Cytoskeleton;Reference proteome SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton " O48802,"PROTEIN NAMES: Protein CLMP1 (CLUMPED CHLOROPLASTS 1) (Protein MADB2) (Protein PHOX2) (Putative myosin adapter B2) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Required for plastid separation and partitioning during cell division. Not involved in plastid constriction or in the organization of cytoplasmic actin cables. Contributes to polar growth of root hairs. KEYWORDS: Cytoplasm;Reference proteome;Repeat;TPR repeat SUBCELLULAR LOCATION: Cytoplasm Note=Localized to distinct foci in the cytoplasm, which frequently colocalize with the cell periphery and with chloroplasts. " O48849,"PROTEIN NAMES: Receptor like protein 23 (AtRLP23) PROTEIN FAMILY: RLP family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the RLP family. FUNCTION: Involved in the perception of necrosis and ethylene-inducing peptide 1-like proteins (NLPs), that act as extracellular signals mediating immune activation. Component of the RLP23-SOBIR1-BAK1 complex that mediates NLP-triggered immunity. KEYWORDS: Cell membrane;Glycoprotein;Leucine-rich repeat;Membrane;Plant defense;Receptor;Reference proteome;Repeat;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein " O48915,"PROTEIN NAMES: Protein NDR1 (Non-race specific disease resistance protein 1) (AtNDR1) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Involved in disease resistance. Required for resistance conferred by multiple R genes recognizing different bacterial and oomycete pathogen isolates like avirulent P.syringae or H.parasitica (downy mildew). Required for the establishment of hypersensitive response (HR) and systemic acquired resistance (SAR) after infection with the bacterial pathogen P.syringae DC3000 carrying avrRpt2. Required for resistance to the soilborne fungus V.longisporum. Interaction with RIN4 is required for the activation of the R gene RPS2 and RPS2-mediated resistance. KEYWORDS: Cell membrane;Glycoprotein;GPI-anchor;Hypersensitive response;Immunity;Innate immunity;Lipoprotein;Membrane;Plant defense;Proteoglycan;Reference proteome;Signal SUBCELLULAR LOCATION: Cell membrane ; Lipid-anchor, GPI-anchor " O49196,"PROTEIN NAMES: Adenylyl-sulfate kinase 2, chloroplastic (ATP adenosine-5'-phosphosulfate 3'-phosphotransferase 2) (Adenosine-5'-phosphosulfate kinase 2) (APS kinase 2) PROTEIN FAMILY: APS kinase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the APS kinase family. FUNCTION: Catalyzes the synthesis of activated sulfate. Essential for plant reproduction and viability. Required for the production of glucosinolates. PATHWAY: Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 2/3. KEYWORDS: Amino-acid biosynthesis;ATP-binding;Chloroplast;Cysteine biosynthesis;Kinase;Nucleotide-binding;Plastid;Reference proteome;Transferase;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast " O49203,"PROTEIN NAMES: Nucleoside diphosphate kinase III, chloroplastic/mitochondrial (NDK III) (NDP kinase III) (NDPK III) PROTEIN FAMILY: NDK family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the NDK family. FUNCTION: Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Shows the highest specificity towards GDP (By similarity). KEYWORDS: ATP-binding;Chloroplast;Direct protein sequencing;Kinase;Magnesium;Metal-binding;Mitochondrion;Nucleotide metabolism;Nucleotide-binding;Plastid;Reference proteome;Thylakoid;Transferase;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid lumen. Mitochondrion intermembrane space. " O49432,"PROTEIN NAMES: Polygalacturonase QRT3 (AtQRT3) (PG QRT3) (Pectinase QRT3) (Protein QUARTET 3) PROTEIN FAMILY: Glycosyl hydrolase 28 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the glycosyl hydrolase 28 family. FUNCTION: Polygalacturonase required for degrading the pollen mother cell wall during microspore development. KEYWORDS: Cell wall;Cell wall biogenesis/degradation;Glycoprotein;Glycosidase;Hydrolase;Reference proteome;Repeat;Secreted;Signal SUBCELLULAR LOCATION: Secreted, cell wall " O49434,"PROTEIN NAMES: Allantoate deiminase (Allantoate amidohydrolase) (AtAAH) PROTEIN FAMILY: Peptidase M20A family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the peptidase M20A family. FUNCTION: Involved in the catabolism of purine nucleotides. Can use allantoate as substrate, but not Nalpha-carbamoyl-L-Asp, Nalpha-carbamoyl-L-Ala or Nalpha-carbamoyl-Gly. The sequential activity of AAH, UGLYAH and UAH allows a complete purine breakdown without the intermediate generation of urea. Involved in the regulation of seed maturation and seed dormancy. KEYWORDS: Endoplasmic reticulum;Glycoprotein;Hydrolase;Manganese;Metal-binding;Purine metabolism;Reference proteome;Signal SUBCELLULAR LOCATION: Endoplasmic reticulum " O49696,"PROTEIN NAMES: Aluminum-activated malate transporter 12 (AtALMT12) (Quick anion channel 1) PROTEIN FAMILY: Aromatic acid exporter family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the aromatic acid exporter (TC 2.A.85) family. FUNCTION: Malate-sensitive anion transporter permeable to chloride, nitrate, sulfate and malate. Involved in dark-, CO(2)-, abscisic acid- and water-deficient-induced stomatal closure. Belongs to the R-type anion channels. KEYWORDS: Alternative splicing;Cell membrane;Chloride;Ion channel;Ion transport;Membrane;Reference proteome;Sulfate transport;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Note=previous research indicates also a not confirmed endomembrane localization. MISCELLANEOUS: Malate functions as a gating modifier as well as a permeating substrate." O50580,"PROTEIN NAMES: D-tagatose 3-epimerase (DTE) (D-ribulose 3-epimerase) (Ketose 3-epimerase) PROTEIN FAMILY: Hyi family ORGANISM: Pseudomonas cichorii SIMILARITY: Belongs to the hyi family. FUNCTION: Catalyzes the epimerization of various ketoses at the C(3) position. It is able to interconvert D-tagatose and D-ribulose to D-sorbose and D-xylulose, respectively. The enzyme is also able to accept other ketopentoses such as D-psicose with lower efficiency. KEYWORDS: 3D-structure;Direct protein sequencing;Isomerase;Manganese;Metal-binding " O52063,"PROTEIN NAMES: N-isopropylammelide isopropyl amidohydrolase PROTEIN FAMILY: Metallo-dependent hydrolases superfamily, N-acyl-D-amino-acid deacylase family ORGANISM: Pseudomonas sp. (strain ADP) SIMILARITY: Belongs to the metallo-dependent hydrolases superfamily. N-acyl-D-amino-acid deacylase family. FUNCTION: Transforms N-isopropylammelide to cyanuric acid and isopropylamine. PATHWAY: Xenobiotic degradation; atrazine degradation; cyanurate from atrazine: step 3/3. KEYWORDS: 3D-structure;Cytoplasm;Hydrolase;Metal-binding;Plasmid;Zinc SUBCELLULAR LOCATION: Cytoplasm " O52793,"PROTEIN NAMES: dTDP-4-dehydro-6-deoxy-alpha-D-glucopyranose 2,3-dehydratase (2,3-dehydratase) PROTEIN FAMILY: Hexose 2,3-dehydratase family ORGANISM: Amycolatopsis orientalis (Nocardia orientalis) SIMILARITY: Belongs to the hexose 2,3-dehydratase family. FUNCTION: Involved in the biosynthesis of the 2,3,6-trideoxysugar L-epivancosamine, the terminal sugar added to the aglycone scaffold of chloroeremomycin, a member of the glycopeptide antibiotics vancomycin family. Catalyzes the removal of the hydroxyl group at position C-2 of the hexose ring of dTDP-4-dehydro-6-deoxy-alpha-D-glucopyranose, and the oxidation of the hydroxyl group at position C-3 to form a carbonyl functionality. The product of the reaction, dTDP-2,6-dideoxy-D-glycero-hex-2-enos-4-ulose, is a highly unstable diketosugar, which spontaneously forms dTDP-3,4-didehydro-2,6-dideoxy-alpha-D-glucose. PATHWAY: Antibiotic biosynthesis. KEYWORDS: 3D-structure;Antibiotic biosynthesis;Disulfide bond;Lyase MISCELLANEOUS: The mutant Ala-381 is used to improve the quality of the crystals.; MISCELLANEOUS: Two binding sites (pockets A and B) for the dTDP-sugar ligands have been identified in each subunit. It seems that pocket A represents the active site and pocket B is a vestige of the gene duplication event." O53181,"PROTEIN NAMES: 2-oxoglutarate oxidoreductase subunit KorB (Alpha-ketoglutarate oxidoreductase subunit beta) (KG oxidoreductase subunit beta) (KGO subunit beta) (KOR subunit beta) ORGANISM: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) FUNCTION: Component of KG oxidoreductase (KOR) that catalyzes the CoA-dependent oxidative decarboxylation of 2-oxoglutarate (alpha-ketoglutarate, KG) to succinyl-CoA. Methyl viologen can act as electron acceptor in vitro; the physiologic electron acceptor is unknown. Is involved in the alternative TCA pathway that functions concurrently with fatty acid beta-oxidation. Since a growing body of evidence indicates that lipids (for example cholesterol and fatty acids) are a predominant growth substrate for M.tuberculosis during infection, flux through KOR likely represents an important step in intermediary metabolism in vivo. KOR-dependent decarboxylation of KG also appears to be an important source of CO(2) in M.tuberculosis metabolism. PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle. KEYWORDS: Magnesium;Oxidoreductase;Reference proteome;Tricarboxylic acid cycle MISCELLANEOUS: Is extremely stable under aerobic conditions." O53896,"PROTEIN NAMES: Serine protease PepD (HtrA-like serine protease) PROTEIN FAMILY: Peptidase S1C family ORGANISM: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) SIMILARITY: Belongs to the peptidase S1C family. FUNCTION: Required for virulence. Acts both as a protease, which degrades and/or refolds damaged substrate targets, and as a chaperone. Plays an important role in the stress response network mediated through the two-component regulatory system MprAB and SigE signaling networks. May utilize its PDZ domain to recognize and process misfolded proteins at the cell membrane, leading to activation of the MprAB and SigE signaling pathways and subsequent establishment of a positive feedback loop that facilitates bacterial adaptation. Interacts with and potentially cleaves several proteins, including the 35 kDa antigen PspA. Proteolytic cleavage of PspA may help to maintain cell envelope homeostasis in Mycobacterium and regulate specific stress response pathways during periods of extracytoplasmic stress. In vitro, exhibits proteolytic activity against the artificial substrate beta-casein. KEYWORDS: 3D-structure;Cell inner membrane;Cell membrane;Cell wall;Hydrolase;Membrane;Protease;Reference proteome;Secreted;Serine protease;Stress response;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell inner membrane ; Single-pass membrane protein Secreted, cell wall Secreted Note=Traffics from the cytoplasm through the cell membrane to the cell wall where it is autoprocessed and eventually secreted into the culture filtrate protein. " O54697,"PROTEIN NAMES: Aminopeptidase NAALADL1 (100 kDa ileum brush border membrane protein) (I100) (Ileal dipeptidylpeptidase) (N-acetylated-alpha-linked acidic dipeptidase-like protein) (NAALADase L) PROTEIN FAMILY: Peptidase M28 family, M28B subfamily ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the peptidase M28 family. M28B subfamily. FUNCTION: Aminopeptidase with broad substrate specificity. Has lower activity with substrates that have Asp or Glu in the P2' position, or Pro in the P3' position. Lacks activity with substrates that have both Pro in the P3' position and Asp or Glu in the P2' position. Lacks carboxypeptidase activity. Lacks dipeptidyl-peptidase IV type activity. KEYWORDS: Aminopeptidase;Calcium;Cell membrane;Direct protein sequencing;Disulfide bond;Glycoprotein;Hydrolase;Membrane;Metal-binding;Metalloprotease;Protease;Reference proteome;Signal-anchor;Transmembrane;Transmembrane helix;Zinc SUBCELLULAR LOCATION: Apical cell membrane ; Single-pass type II membrane protein Note=Ileal brush border membrane. " O54775,"PROTEIN NAMES: CCN family member 4 (ELM-1) (WNT1-inducible-signaling pathway protein 1) (WISP-1) PROTEIN FAMILY: CCN family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the CCN family. FUNCTION: Downstream regulator in the Wnt/Frizzled-signaling pathway (By similarity). Associated with cell survival. Adheres to skin and melanoma fibroblasts (By similarity). In vitro binding to skin fibroblasts occurs through the proteoglycans, decorin and biglycan (By similarity). Suppresses tumor growth in vivo. KEYWORDS: Cell adhesion;Disulfide bond;Glycoprotein;Proto-oncogene;Reference proteome;Secreted;Signal;Wnt signaling pathway SUBCELLULAR LOCATION: Secreted " O54834,PROTEIN NAMES: Rho GTPase-activating protein 6 (Rho-type GTPase-activating protein 6) (Rho-type GTPase-activating protein RhoGAPX-1) ORGANISM: Mus musculus (Mouse) FUNCTION: GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state. Could regulate the interactions of signaling molecules with the actin cytoskeleton. Promotes continuous elongation of cytoplasmic processes during cell motility and simultaneous retraction of the cell body changing the cell morphology (By similarity). KEYWORDS: Alternative splicing;Cytoplasm;GTPase activation;Phosphoprotein;Reference proteome;SH3-binding SUBCELLULAR LOCATION: Cytoplasm O54912,"PROTEIN NAMES: Potassium channel subfamily K member 3 (Acid-sensitive potassium channel protein TASK-1) (TWIK-related acid-sensitive K(+) channel 1) (Two pore potassium channel KT3.1) (Two pore K(+) channel KT3.1) PROTEIN FAMILY: Two pore domain potassium channel family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the two pore domain potassium channel (TC 1.A.1.8) family. FUNCTION: pH-dependent, voltage-insensitive, background potassium channel protein. Rectification direction results from potassium ion concentration on either side of the membrane. Acts as an outward rectifier when external potassium concentration is low. When external potassium concentration is high, current is inward. KEYWORDS: Cell membrane;Glycoprotein;Ion channel;Ion transport;Membrane;Potassium;Potassium channel;Potassium transport;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein MISCELLANEOUS: Inhibited by extracellular acidification, zinc, bupivacaine and phenytoin. Activated by protein kinase A." O54946,"PROTEIN NAMES: DnaJ homolog subfamily B member 6 (Heat shock protein J2) (HSJ-2) (MRJ) (mDj4) ORGANISM: Mus musculus (Mouse) FUNCTION: Has a stimulatory effect on the ATPase activity of HSP70 in a dose-dependent and time-dependent manner and hence acts as a co-chaperone of HSP70. Plays an indispensable role in the organization of KRT8/KRT18 filaments. Acts as an endogenous molecular chaperone for neuronal proteins including huntingtin. Suppresses aggregation and toxicity of polyglutamine-containing, aggregation-prone proteins. Also reduces cellular toxicity and caspase-3 activity (By similarity). KEYWORDS: Alternative splicing;Chaperone;Cytoplasm;Methylation;Nucleus;Reference proteome SUBCELLULAR LOCATION: Cytoplasm, perinuclear region Nucleus Cytoplasm, myofibril, sarcomere, Z line " O54990,"PROTEIN NAMES: Prominin-1 (Antigen AC133 homolog) (Prominin-like protein 1) (CD antigen CD133) PROTEIN FAMILY: Prominin family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the prominin family. FUNCTION: May play a role in cell differentiation, proliferation and apoptosis. Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner. KEYWORDS: Acetylation;Alternative splicing;Cell membrane;Cell projection;Cilium;Endoplasmic reticulum;Glycoprotein;Membrane;Phosphoprotein;Reference proteome;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Apical cell membrane; Multi-pass membrane protein. Cell projection, microvillus membrane; Multi-pass membrane protein. Cell projection, cilium, photoreceptor outer segment. Endoplasmic reticulum Endoplasmic reticulum-Golgi intermediate compartment Note=Found in extracellular membrane particles in various body fluids such as ventricular fluid of the developing brain and urine. MISCELLANEOUS: Fundus images and light microscopy of retinal sections from transgenic mice expressing mutant PROM1 reveal progressive retinal abnormalities visible as subretinal deposits and photoreceptor atrophy." O54992,"PROTEIN NAMES: MAP kinase-activated protein kinase 5 (MAPK-activated protein kinase 5) (MAPKAP kinase 5) (MAPKAPK-5) PROTEIN FAMILY: Protein kinase superfamily, CAMK Ser/Thr protein kinase family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. FUNCTION: Tumor suppressor serine/threonine-protein kinase involved in mTORC1 signaling and post-transcriptional regulation. Phosphorylates FOXO3, ERK3/MAPK6, ERK4/MAPK4, HSP27/HSPB1, p53/TP53 and RHEB. Acts as a tumor suppressor by mediating Ras-induced senescence and phosphorylating p53/TP53. Involved in post-transcriptional regulation of MYC by mediating phosphorylation of FOXO3: phosphorylation of FOXO3 leads to promote nuclear localization of FOXO3, enabling expression of miR-34b and miR-34c, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent MYC translation. Acts as a negative regulator of mTORC1 signaling by mediating phosphorylation and inhibition of RHEB. Part of the atypical MAPK signaling via its interaction with ERK3/MAPK6 or ERK4/MAPK4: the precise role of the complex formed with ERK3/MAPK6 or ERK4/MAPK4 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPK (ERK3/MAPK6 or ERK4/MAPK4), ERK3/MAPK6 (or ERK4/MAPK4) is phosphorylated and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK3/MAPK6 (or ERK4/MAPK4). Mediates phosphorylation of HSP27/HSPB1 in response to PKA/PRKACA stimulation, inducing F-actin rearrangement. KEYWORDS: Alternative splicing;ATP-binding;Coiled coil;Cytoplasm;Kinase;Nucleotide-binding;Nucleus;Phosphoprotein;Reference proteome;Serine/threonine-protein kinase;Transferase;Tumor suppressor SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Translocates to the cytoplasm following phosphorylation and activation. Interaction with ERK3/MAPK6 or ERK4/MAPK4 and phosphorylation at Thr-182, activates the protein kinase activity, followed by translocation to the cytoplasm. Phosphorylation by PKA/PRKACA at Ser-115 also induces nuclear export. " O55082,"PROTEIN NAMES: Tyrosine-protein phosphatase non-receptor type 20 (Testis-specific tyrosine phosphatase) PROTEIN FAMILY: Protein-tyrosine phosphatase family, Non-receptor class subfamily ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the protein-tyrosine phosphatase family. Non-receptor class subfamily. FUNCTION: Tyrosine-protein phosphatase targeted to sites of actin polymerization in response of varied extracellular stimuli. Has tyrosine phosphatase activity towards various tyrosyl phosphorylated substrates. KEYWORDS: Alternative splicing;Cytoplasm;Cytoskeleton;Hydrolase;Microtubule;Nucleus;Phosphoprotein;Protein phosphatase;Reference proteome SUBCELLULAR LOCATION: Nucleus Cytoplasm Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Note=Colocalizes with the microtubule-organizing center and intracellular membrane compartments. " O55100,"PROTEIN NAMES: Synaptogyrin-1 PROTEIN FAMILY: Synaptogyrin family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the synaptogyrin family. FUNCTION: May play a role in regulated exocytosis. Modulates the localization of synaptophysin/SYP into synaptic-like microvesicles and may therefore play a role in synaptic-like microvesicle formation and/or maturation (By similarity). Involved in the regulation of short-term and long-term synaptic plasticity. KEYWORDS: Acetylation;Alternative splicing;Cytoplasmic vesicle;Direct protein sequencing;Membrane;Reference proteome;Synapse;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane ; Multi-pass membrane protein Melanosome " O55242,"PROTEIN NAMES: Sigma non-opioid intracellular receptor 1 (Sigma 1-type opioid receptor) (Sigma1-receptor) (Sigma1R) PROTEIN FAMILY: ERG2 family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the ERG2 family. FUNCTION: Functions in lipid transport from the endoplasmic reticulum and is involved in a wide array of cellular functions probably through regulation of the biogenesis of lipid microdomains at the plasma membrane. Involved in the regulation of different receptors it plays a role in BDNF signaling and EGF signaling. Also regulates ion channels like the potassium channel and could modulate neurotransmitter release. Plays a role in calcium signaling through modulation together with ANK2 of the ITP3R-dependent calcium efflux at the endoplasmic reticulum. Plays a role in several other cell functions including proliferation, survival and death. Originally identified for its ability to bind various psychoactive drugs it is involved in learning processes, memory and mood alteration. Necessary for proper mitochondrial axonal transport in motor neurons, in particular the retrograde movement of mitochondria. Plays a role in protecting cells against oxidative stress-induced cell death via its interaction with RNF112. KEYWORDS: Alternative splicing;Cell junction;Cell membrane;Cell projection;Cytoplasmic vesicle;Endoplasmic reticulum;Lipid droplet;Lipid transport;Membrane;Nucleus;Postsynaptic cell membrane;Receptor;Reference proteome;Synapse;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Nucleus inner membrane Nucleus outer membrane Nucleus envelope Cytoplasmic vesicle Endoplasmic reticulum membrane Membrane ; Single-pass membrane protein Lipid droplet Cell junction Cell membrane Cell projection, growth cone Postsynaptic density membrane Note=During interphase, detected at the inner and outer nuclear membrane and the endoplasmic reticulum. Detected on cytoplasmic vesicles during mitosis (By similarity). Targeted to lipid droplets, cholesterol and galactosylceramide-enriched domains of the endoplasmic reticulum. Enriched at cell-cell communication regions, growth cone and postsynaptic structures. Localization is modulated by ligand-binding. In motor neurons it is enriched at cholinergic postsynaptic densities. MISCELLANEOUS: Depletion by RNAi enhances kappa-type opioid receptor-mediated analgesia and prevents the memory-improving effects of (-)- and (+)-pentazocine.; MISCELLANEOUS: Sigma receptors are classified into two subtypes (Sigma-1 and Sigma-2) based on their different pharmacological profile." O57604,"PROTEIN NAMES: Podocalyxin (Podocalyxin-like protein 1) (PC) (PCLP-1) (Thrombomucin) PROTEIN FAMILY: Podocalyxin family ORGANISM: Gallus gallus (Chicken) SIMILARITY: Belongs to the podocalyxin family. FUNCTION: Involved in the regulation of both adhesion and cell morphology and cancer progression. Functions as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. Involved in the formation of a preapical plasma membrane subdomain to set up initial epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR-dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells (By similarity). Induces the formation of apical actin-dependent microvilli. KEYWORDS: Cell adhesion;Cell membrane;Cell projection;Direct protein sequencing;Glycoprotein;Membrane;Reference proteome;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Apical cell membrane Cell projection, microvillus Membrane raft Cell projection, lamellipodium Cell projection, filopodium Cell projection, ruffle Membrane ; Single-pass type I membrane protein Note=In single attached epithelial cells is restricted to a preapical pole on the free plasma membrane whereas other apical and basolateral proteins are not yet polarized. Colocalizes with NHERF2 at the apical plasma membrane during epithelial polarization. Colocalizes with NHERF1 at the trans-Golgi network (transiently). Its association with the membrane raft is transient. Forms granular, punctuated pattern, forming patches, preferentially adopting a polar distribution, located on the migrating poles of the cell or forming clusters along the terminal ends of filipodia establishing contact with the endothelial cells. Colocalizes with the submembrane actin of lamellipodia, particularly associated with ruffles. Colocalizes with vinculin at protrusions of cells. Colocalizes with ITGB1. Colocalizes with actin filaments, ezrin and NHERF1 in a punctate pattern at the apical cell surface where microvilli form. Colocalizes with EZR and NHERF2 at the apical cell membrane of glomerular epithelium cells (By similarity). Colocalizes with NHERF1 at the apical cell membrane. Colocalizes with PARD3, PRKCI, EXOC5, OCLN, RAB11A and RAB8A in apical membrane initiation sites (AMIS) during the generation of apical surface and luminogenesis (By similarity). " O57712,"PROTEIN NAMES: tRNA (cytosine(72)-C(5))-methyltransferase (tRNA:m(5)C72 MTase) (PhNSun6) PROTEIN FAMILY: Class I-like SAM-binding methyltransferase superfamily, RsmB/NOP family ORGANISM: Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family. FUNCTION: S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the C5 position of cytosine 72 in several tRNAs. This modification appears to slightly promote the thermal stability of P.horikoshii tRNAs, but does not affect their amino acid accepting activity. Four elements in the acceptor stems of tRNAs are essential for substrate recognition by this enzyme: the target site C72, the 3'-CCA terminus, U73 or G73, and the second base pair C2:G71. KEYWORDS: 3D-structure;Methyltransferase;RNA-binding;S-adenosyl-L-methionine;Transferase " O57878,"PROTEIN NAMES: Broad substrate specificity amino-acid racemase (BAR) PROTEIN FAMILY: Class-III pyridoxal-phosphate-dependent aminotransferase family ORGANISM: Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) SIMILARITY: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. FUNCTION: Amino-acid racemase able to utilize a broad range of substrates. Can use Met, Leu, Phe, Ala, Ser, Ile, Val, Trp, Tyr and Thr. Is mostly active with Phe, Leu, Met and Tyr, followed by Ile, Thr and Trp. Has weaker activity with Val, Ser and Ala. Shows no activity toward Pro, Asp, Glu, Arg, His, Gln and Asn. KEYWORDS: Isomerase;Pyridoxal phosphate " O58440,PROTEIN NAMES: Endoribonuclease Nob1 (RNase Nob1) (Endonuclease VapC6) (Putative toxin VapC6) PROTEIN FAMILY: PINc/VapC protein family ORGANISM: Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) SIMILARITY: Belongs to the PINc/VapC protein family. FUNCTION: Toxic component of a type II toxin-antitoxin (TA) system (Potential). Processes pre-16S-rRNA at its 3' end (the D-site) to yield the mature 3' end. KEYWORDS: 3D-structure;Endonuclease;Hydrolase;Manganese;Metal-binding;Nuclease;Ribosome biogenesis;RNA-binding;rRNA-binding;Toxin-antitoxin system;Zinc O58478,"PROTEIN NAMES: Alanine/serine racemase (ASR) (Ala/Ser racemase) PROTEIN FAMILY: Class-III pyridoxal-phosphate-dependent aminotransferase family ORGANISM: Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) SIMILARITY: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. FUNCTION: Catalyzes the interconversion of L-alanine and D-alanine, and L-serine and D-serine. Has weak activity with valine and threonine. KEYWORDS: Isomerase;Pyridoxal phosphate " O59179,PROTEIN NAMES: Membrane-bound protease PH1510 (NfeD homolog) (Stomatin operon partner protein) (STOPP) PROTEIN FAMILY: Peptidase S14 family ORGANISM: Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) SIMILARITY: Belongs to the peptidase S14 family. FUNCTION: Protease that cleaves its substrates preferentially near hydrophobic or aromatic amino acid residues. Can degrade casein and the stomatin homolog PH1511 (in vitro). KEYWORDS: 3D-structure;Hydrolase;Membrane;Protease;Serine protease;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Multi-pass membrane protein O59196,"PROTEIN NAMES: Tetrahedral aminopeptidase (TET) (TET aminopeptidase) (Leucyl aminopeptidase) (PhTET2) PROTEIN FAMILY: Peptidase M42 family ORGANISM: Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) SIMILARITY: Belongs to the peptidase M42 family. FUNCTION: Functions as an aminopeptidase, with a clear preference for leucine as the N-terminal amino acid. However, can also cleave moderately long polypeptide substrates of various compositions in a fairly unspecific manner. Has neither carboxypeptidase nor endoproteolytic activities, and it is devoid of N-terminal deblocking activity. Is involved in protein degradation, performing degradation of oligopeptides produced by the proteasome into single amino acids. KEYWORDS: 3D-structure;Aminopeptidase;Cobalt;Hydrolase;Metal-binding;Metalloprotease;Protease;Zinc MISCELLANEOUS: The hydrolytic mechanism is nonprocessive. Therefore, the enzyme does not process one substrate molecule completely before starting with another one. Instead, the reaction products are generated by multiple rounds of substrate digestion." O59726,PROTEIN NAMES: Vacuolar membrane amino acid uptake transporter fnx2 PROTEIN FAMILY: Major facilitator superfamily ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the major facilitator superfamily. FUNCTION: MFS-type transporter involved in vacuolar amino acid uptake. KEYWORDS: Membrane;Reference proteome;Transmembrane;Transmembrane helix;Transport;Vacuole SUBCELLULAR LOCATION: Vacuole Membrane ; Multi-pass membrane protein O59747,PROTEIN NAMES: Palmitoyl-protein thioesterase-dolichyl pyrophosphate phosphatase fusion 1 [Cleaved into: Palmitoyl-protein thioesterase (PPT) (Palmitoyl-protein hydrolase); Dolichyldiphosphatase (Dolichyl pyrophosphate phosphatase)] PROTEIN FAMILY: Palmitoyl-protein thioesterase family; Dolichyldiphosphatase family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: In the N-terminal section; belongs to the palmitoyl-protein thioesterase family.; SIMILARITY: In the C-terminal section; belongs to the dolichyldiphosphatase family. FUNCTION: Essential protein. Removes thioester-linked fatty acyl groups such as palmitate from modified cysteine residues in proteins or peptides during vacuolar degradation. Required for efficient N-glycosylation. Necessary for maintaining optimal levels of dolichol-linked oligosaccharides. KEYWORDS: Disulfide bond;Endoplasmic reticulum;Glycoprotein;Hydrolase;Membrane;Reference proteome;Signal;Transmembrane;Transmembrane helix;Vacuole SUBCELLULAR LOCATION: Vacuole Endoplasmic reticulum membrane ; Multi-pass membrane protein O59751,"PROTEIN NAMES: Kinesin-like protein 6 PROTEIN FAMILY: TRAFAC class myosin-kinesin ATPase superfamily, Kinesin family, Kinesin II subfamily ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. Kinesin II subfamily. FUNCTION: Has a role in establishing metaphase during mitosis. Required for chromosome segregation where it generates tension during kinetochore capturing. KEYWORDS: ATP-binding;Cell cycle;Cell division;Centromere;Chromosome;Chromosome partition;Coiled coil;Cytoplasm;Cytoskeleton;Kinetochore;Microtubule;Mitosis;Motor protein;Nucleotide-binding;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Cytoplasm, cytoskeleton Chromosome, centromere, kinetochore Cytoplasm, cytoskeleton, spindle Note=Cytoplasmic microtubules in interphase, mitotic kinetochores in metaphase and spindle midzone in anaphase and telophase. " O59763,"PROTEIN NAMES: Serine/threonine-protein kinase oca2 PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. FUNCTION: Overexpression causes cell cycle arrest. KEYWORDS: ATP-binding;Cell cycle;Cytoplasm;Kinase;Nucleotide-binding;Phosphoprotein;Reference proteome;Serine/threonine-protein kinase;Transferase SUBCELLULAR LOCATION: Cytoplasm Note=Barrier septum. Cell tip. " O59767,"PROTEIN NAMES: Mitotic spindle checkpoint component mad3 ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) FUNCTION: Has a role in transducing the anaphase inhibitory signal to the anaphase promoting complex (APC). Forms part of the mad2 feedback control. KEYWORDS: 3D-structure;Cell cycle;Cell division;Centromere;Chromosome;Kinetochore;Mitosis;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus Chromosome, centromere, kinetochore Note=Associated with the kinetochore. " O59923,"PROTEIN NAMES: NAD-dependent histone deacetylase SIR2 (Regulatory protein SIR2) (Silent information regulator 2) PROTEIN FAMILY: Sirtuin family, Class I subfamily ORGANISM: Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) SIMILARITY: Belongs to the sirtuin family. Class I subfamily. FUNCTION: NAD-dependent deacetylase. Heterochromatin component that silences transcription at silent mating loci, telomeres and the ribosomal DNA, and that also suppresses recombination in the rDNA and extends replicative life span. It acts as a NAD-dependent histone deacetylase, which deacetylates 'Lys-9' and 'Lys-14' of Histone H3 and 'Lys-16' of Histone H4. Functions in the distribution of oxidatively damaged proteins during cell division. Mediates phenotypic switching. KEYWORDS: Metal-binding;NAD;Nucleus;Reference proteome;Repressor;Transcription;Transcription regulation;Transferase;Zinc SUBCELLULAR LOCATION: Nucleus " O60052,PROTEIN NAMES: Protein farnesyltransferase/geranylgeranyltransferase type-1 subunit alpha (CAAX farnesyltransferase subunit alpha) (FTase-alpha) (Ras proteins prenyltransferase subunit alpha) (Type I protein geranyl-geranyltransferase subunit alpha) (GGTase-I-alpha) PROTEIN FAMILY: Protein prenyltransferase subunit alpha family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the protein prenyltransferase subunit alpha family. FUNCTION: Catalyzes the transfer of a farnesyl or geranyl-geranyl moiety from farnesyl or geranyl-geranyl diphosphate to a cysteine at the fourth position from the C-terminus of several proteins having the C-terminal sequence Cys-aliphatic-aliphatic-X. The alpha(cwp1) subunit is thought to participate in a stable complex with the substrate. The beta(cpp1 or cwg2) subunits bind the peptide substrate. KEYWORDS: Magnesium;Prenyltransferase;Reference proteome;Repeat;Transferase O60119,"PROTEIN NAMES: Vesicle-associated membrane protein-associated protein scs2 (VAMP-associated protein scs2) (VAP homolog 1) PROTEIN FAMILY: VAMP-associated protein (VAP) family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the VAMP-associated protein (VAP) (TC 9.B.17) family. FUNCTION: Vesicle-associated membrane protein-associated protein (VAP) implicated in maintaining the cortical endoplasmic reticulum (ER)-plasma membrane (PM) attachment. ER-PM contacts function to modulate the distribution of contractile ring components to ensure robust ring assembly. ER-PM contacts function also in controlling exocytosis and maintenance of cell polarity regulating cell shape. VAPs play an important role in regulating eisosome assembly. VAPs also contribute to ER-phagy by tethering atg8 to the ER membrane, but also by maintaining the ER-plasma membrane contact (Ref.8). KEYWORDS: Endoplasmic reticulum;Membrane;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Single-pass type IV membrane protein Note=Localizes at the cortical endoplasmic reticulum-plasma membrane contact sites. " O60126,"PROTEIN NAMES: DNA topoisomerase 3 (DNA topoisomerase III) PROTEIN FAMILY: Type IA topoisomerase family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the type IA topoisomerase family. FUNCTION: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). KEYWORDS: DNA-binding;Isomerase;Reference proteome;Topoisomerase " O60129,"PROTEIN NAMES: Fork head protein homolog 2 ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) FUNCTION: Required for promoter sequence element PCB-driven, M-phase-specific transcription. Acts as a transcriptional activator with a role in the regulation of mitosis. Binds, cooperatively with mcm1, the CLB cluster regulatory elements throughout the cell cycle. Regulates the periodic transcription of cdc15 and spo12. Required for the correct timing, positioning and contraction of the division septum. KEYWORDS: Activator;Cell cycle;Cell division;DNA-binding;Mitosis;Nucleus;Phosphoprotein;Reference proteome;Septation;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " O60139,"PROTEIN NAMES: Probable ubiquitin carboxyl-terminal hydrolase 4 (Deubiquitinating enzyme 4) (Ubiquitin thioesterase 4) (Ubiquitin-specific-processing protease 4) PROTEIN FAMILY: Peptidase C19 family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the peptidase C19 family. FUNCTION: Has an ATP-independent isopeptidase activity, cleaving at the C-terminus of the ubiquitin moiety. Acts late in the proteolytic pathway in conjunction with the 26S proteasome. Plays a role in avoiding DNA overreplication (By similarity). KEYWORDS: Cytoplasm;Endosome;Hydrolase;Phosphoprotein;Protease;Reference proteome;Thiol protease;Ubl conjugation pathway SUBCELLULAR LOCATION: Cytoplasm. Endosome. " O60235,"PROTEIN NAMES: Transmembrane protease serine 11D (Airway trypsin-like protease) [Cleaved into: Transmembrane protease serine 11D non-catalytic chain; Transmembrane protease serine 11D catalytic chain] PROTEIN FAMILY: Peptidase S1 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the peptidase S1 family. FUNCTION: May play some biological role in the host defense system on the mucous membrane independently of or in cooperation with other substances in airway mucous or bronchial secretions. Plays a role in the proteolytic processing of ACE2. Proteolytically cleaves and activates the human coronavirus 229E (HCoV-229E) spike glycoprotein which facilitate virus-cell membrane fusions; spike proteins are synthesized and maintained in precursor intermediate folding states and proteolysis permits the refolding and energy release required to create stable virus-cell linkages and membrane coalescence. Preferentially cleaves the C-terminal side of arginine residues at the P1 position of certain peptides, cleaving Boc-Phe-Ser-Arg-4-methylcoumaryl-7-amide most efficiently and having an optimum pH of 8.6 with this substrate. KEYWORDS: Cell membrane;Direct protein sequencing;Disulfide bond;Glycoprotein;Hydrolase;Membrane;Protease;Reference proteome;Secreted;Serine protease;Signal-anchor;Transmembrane;Transmembrane helix;Zymogen SUBCELLULAR LOCATION: Cell membrane; Single-pass type II membrane protein. Note=Activated by cleavage and secreted.; SUBCELLULAR LOCATION: [Transmembrane protease serine 11D catalytic chain]: Secreted. Note=Activated by cleavage and secreted. " O60260,"PROTEIN NAMES: E3 ubiquitin-protein ligase parkin (Parkin) (Parkin RBR E3 ubiquitin-protein ligase) (Parkinson juvenile disease protein 2) (Parkinson disease protein 2) PROTEIN FAMILY: RBR family, Parkin subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the RBR family. Parkin subfamily. FUNCTION: Functions within a multiprotein E3 ubiquitin ligase complex, catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. Substrates include SYT11 and VDAC1. Other substrates are BCL2, CCNE1, GPR37, RHOT1/MIRO1, MFN1, MFN2, STUB1, SNCAIP, SEPTIN5, TOMM20, USP30, ZNF746, MIRO1 and AIMP2. Mediates monoubiquitination as well as 'Lys-6', 'Lys-11', 'Lys-48'-linked and 'Lys-63'-linked polyubiquitination of substrates depending on the context. Participates in the removal and/or detoxification of abnormally folded or damaged protein by mediating 'Lys-63'-linked polyubiquitination of misfolded proteins such as PARK7: 'Lys-63'-linked polyubiquitinated misfolded proteins are then recognized by HDAC6, leading to their recruitment to aggresomes, followed by degradation. Mediates 'Lys-63'-linked polyubiquitination of a 22 kDa O-linked glycosylated isoform of SNCAIP, possibly playing a role in Lewy-body formation. Mediates monoubiquitination of BCL2, thereby acting as a positive regulator of autophagy. Protects against mitochondrial dysfunction during cellular stress, by acting downstream of PINK1 to coordinate mitochondrial quality control mechanisms that remove and replace dysfunctional mitochondrial components. Depending on the severity of mitochondrial damage and/or dysfunction, activity ranges from preventing apoptosis and stimulating mitochondrial biogenesis to regulating mitochondrial dynamics and eliminating severely damaged mitochondria via mitophagy. Activation and recruitment onto the outer membrane of damaged/dysfunctional mitochondria (OMM) requires PINK1-mediated phosphorylation of both PRKN and ubiquitin. After mitochondrial damage, functions with PINK1 to mediate the decision between mitophagy or preventing apoptosis by inducing either the poly- or monoubiquitination of VDAC1, respectively; polyubiquitination of VDAC1 promotes mitophagy, while monoubiquitination of VDAC1 decreases mitochondrial calcium influx which ultimately inhibits apoptosis. When cellular stress results in irreversible mitochondrial damage, promotes the autophagic degradation of dysfunctional depolarized mitochondria (mitophagy) by promoting the ubiquitination of mitochondrial proteins such as TOMM20, RHOT1/MIRO1, MFN1 and USP30. Preferentially assembles 'Lys-6'-, 'Lys-11'- and 'Lys-63'-linked polyubiquitin chains, leading to mitophagy. The PINK1-PRKN pathway also promotes fission of damaged mitochondria by PINK1-mediated phosphorylation which promotes the PRKN-dependent degradation of mitochondrial proteins involved in fission such as MFN2. This prevents the refusion of unhealthy mitochondria with the mitochondrial network or initiates mitochondrial fragmentation facilitating their later engulfment by autophagosomes. Regulates motility of damaged mitochondria via the ubiquitination and subsequent degradation of MIRO1 and MIRO2; in motor neurons, this likely inhibits mitochondrial intracellular anterograde transport along the axons which probably increases the chance of the mitochondria undergoing mitophagy in the soma. Involved in mitochondrial biogenesis via the 'Lys-48'-linked polyubiquitination of transcriptional repressor ZNF746/PARIS which leads to its subsequent proteasomal degradation and allows activation of the transcription factor PPARGC1A. Limits the production of reactive oxygen species (ROS). Regulates cyclin-E during neuronal apoptosis. In collaboration with CHPF isoform 2, may enhance cell viability and protect cells from oxidative stress. Independently of its ubiquitin ligase activity, protects from apoptosis by the transcriptional repression of p53/TP53. May protect neurons against alpha synuclein toxicity, proteasomal dysfunction, GPR37 accumulation, and kainate-induced excitotoxicity. May play a role in controlling neurotransmitter trafficking at the presynaptic terminal and in calcium-dependent exocytosis. May represent a tumor suppressor gene. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: 3D-structure;Alternative splicing;Autophagy;Cell projection;Cytoplasm;Disease variant;Endoplasmic reticulum;Isopeptide bond;Membrane;Metal-binding;Mitochondrion;Mitochondrion outer membrane;Neurodegeneration;Nucleus;Parkinson disease;Parkinsonism;Phosphoprotein;Reference proteome;Repeat;S-nitrosylation;Synapse;Transcription;Transcription regulation;Transferase;Ubl conjugation;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cytoplasm, cytosol Nucleus Endoplasmic reticulum Mitochondrion Mitochondrion outer membrane Cell projection, neuron projection Postsynaptic density Presynapse Note=Mainly localizes in the cytosol. Co-localizes with SYT11 in neutrites. Co-localizes with SNCAIP in brainstem Lewy bodies. Translocates to dysfunctional mitochondria that have lost the mitochondrial membrane potential; recruitment to mitochondria is PINK1-dependent. Mitochondrial localization also gradually increases with cellular growth. MISCELLANEOUS: The parkin locus (PRKN), adjacent to the 6q telomere is hyper-recombinable and lies within FRA6E, the third most common fragile site in tumor tissue." O60337,"PROTEIN NAMES: E3 ubiquitin-protein ligase MARCHF6 (Doa10 homolog) (Membrane-associated RING finger protein 6) (Membrane-associated RING-CH protein VI) (MARCH-VI) (Protein TEB-4) (RING finger protein 176) (RING-type E3 ubiquitin transferase MARCHF6) ORGANISM: Homo sapiens (Human) FUNCTION: E3 ubiquitin-protein ligase that promotes 'Lys-48'-linked ubiquitination of target proteins, leading to their proteasomal degradation. Promotes ubiquitination of DIO2, leading to its degradation. Promotes ubiquitination of SQLE, leading to its degradation. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. May cooperate with UBE2G1. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Acetylation;Alternative splicing;Endoplasmic reticulum;Epilepsy;Membrane;Metal-binding;Reference proteome;Transferase;Transmembrane;Transmembrane helix;Ubl conjugation;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein " O60508,PROTEIN NAMES: Pre-mRNA-processing factor 17 (Cell division cycle 40 homolog) (EH-binding protein 3) (Ehb3) (PRP17 homolog) (hPRP17) ORGANISM: Homo sapiens (Human) FUNCTION: Required for pre-mRNA splicing as component of the activated spliceosome. Plays an important role in embryonic brain development; this function does not require proline isomerization. KEYWORDS: 3D-structure;Disease variant;Intellectual disability;mRNA processing;mRNA splicing;Nucleus;Phosphoprotein;Reference proteome;Repeat;Spliceosome;WD repeat SUBCELLULAR LOCATION: Nucleus Nucleus speckle O60548,PROTEIN NAMES: Forkhead box protein D2 (Forkhead-related protein FKHL17) (Forkhead-related transcription factor 9) (FREAC-9) ORGANISM: Homo sapiens (Human) FUNCTION: Probable transcription factor involved in embryogenesis and somatogenesis. KEYWORDS: DNA-binding;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus O60635,"PROTEIN NAMES: Tetraspanin-1 (Tspan-1) (Tetraspan NET-1) (Tetraspanin TM4-C) PROTEIN FAMILY: Tetraspanin (TM4SF) family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the tetraspanin (TM4SF) family. FUNCTION: Structural component of specialized membrane microdomains known as tetraspanin-enriched microdomains (TERMs), which act as platforms for receptor clustering and signaling. Participates thereby in diverse biological functions such as cell signal transduction, adhesion, migration and protein trafficking. Regulates neuronal differentiation in response to NGF by facilitating NGF-mediated activation of NTRK1/TRKA receptor tyrosine kinase and subsequent downstream signaling pathways (By similarity). Plays a role in the inhibition of TNFalpha-induced apoptosis. Mechanistically, inhibits the NF-kappa-B signaling pathway by blocking phosphorylation of CHUK. Promotes also the stability of the thiamine transporter 1/SLC19A2 in intestinal epithelial cells leading to an increase of thiamine uptake process. KEYWORDS: Cell membrane;Glycoprotein;Lysosome;Membrane;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Lysosome membrane ; Multi-pass membrane protein " O60636,PROTEIN NAMES: Tetraspanin-2 (Tspan-2) (Tetraspan NET-3) PROTEIN FAMILY: Tetraspanin (TM4SF) family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the tetraspanin (TM4SF) family. FUNCTION: May play a role in signalling in oligodendrocytes in the early stages of their terminal differentiation into myelin-forming glia and may also function in stabilizing the mature sheath. KEYWORDS: Alternative splicing;Glycoprotein;Membrane;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Multi-pass membrane protein O60663,PROTEIN NAMES: LIM homeobox transcription factor 1-beta (LIM/homeobox protein 1.2) (LMX-1.2) (LIM/homeobox protein LMX1B) ORGANISM: Homo sapiens (Human) FUNCTION: Transcription factor involved in the regulation of podocyte-expressed genes. Essential for the specification of dorsal limb fate at both the zeugopodal and autopodal levels. KEYWORDS: Activator;Alternative splicing;Developmental protein;Disease variant;DNA-binding;Homeobox;LIM domain;Metal-binding;Nucleus;Reference proteome;Repeat;Transcription;Transcription regulation;Zinc SUBCELLULAR LOCATION: Nucleus O60759,"PROTEIN NAMES: Cytohesin-interacting protein (Cytohesin binder and regulator) (CYBR) (Cytohesin-associated scaffolding protein) (CASP) (Cytohesin-binding protein HE) (Cbp HE) (Pleckstrin homology Sec7 and coiled-coil domains-binding protein) ORGANISM: Homo sapiens (Human) FUNCTION: By its binding to cytohesin-1 (CYTH1), it modifies activation of ARFs by CYTH1 and its precise function may be to sequester CYTH1 in the cytoplasm. KEYWORDS: 3D-structure;Alternative splicing;Coiled coil;Cytoplasm;Endosome;Reference proteome SUBCELLULAR LOCATION: Cytoplasm. Early endosome. Note=Recruited from the cytosol to endosomes by SNX27. " O60806,"PROTEIN NAMES: T-box transcription factor TBX19 (T-box protein 19) (T-box factor, pituitary) ORGANISM: Homo sapiens (Human) FUNCTION: Transcriptional regulator involved in developmental processes. Can activate POMC gene expression and repress the alpha glycoprotein subunit and thyroid-stimulating hormone beta promoters. KEYWORDS: Activator;Disease variant;DNA-binding;Nucleus;Reference proteome;Repressor;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " O60870,"PROTEIN NAMES: DNA/RNA-binding protein KIN17 (Binding to curved DNA) (KIN, antigenic determinant of recA protein homolog) PROTEIN FAMILY: KIN17 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the KIN17 family. FUNCTION: Involved in DNA replication and the cellular response to DNA damage. May participate in DNA replication factories and create a bridge between DNA replication and repair mediated by high molecular weight complexes. May play a role in illegitimate recombination and regulation of gene expression. May participate in mRNA processing. Binds, in vitro, to double-stranded DNA. Also shown to bind preferentially to curved DNA in vitro and in vivo (By similarity). Binds via its C-terminal domain to RNA in vitro. KEYWORDS: 3D-structure;Alternative splicing;Coiled coil;Cytoplasm;Direct protein sequencing;DNA damage;DNA recombination;DNA repair;DNA replication;DNA-binding;Host-virus interaction;Metal-binding;Methylation;mRNA processing;Nucleus;Reference proteome;RNA-binding;Stress response;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Cytoplasm Note=During S phase, strongly associated with the nuclear matrix, and to chromosomal DNA in the presence of DNA damage. Also shows cytoplasmic localization in elongated spermatids. MISCELLANEOUS: Recognized by antibodies directed against the RecA protein." O60921,"PROTEIN NAMES: Checkpoint protein HUS1 (hHUS1) PROTEIN FAMILY: HUS1 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the HUS1 family. FUNCTION: Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. KEYWORDS: 3D-structure;Alternative splicing;Cytoplasm;DNA damage;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus Cytoplasm, cytosol Note=In discrete nuclear foci upon DNA damage. According to previous research, localized also in the cytoplasm. DNA damage induces its nuclear translocation. Shuttles between the nucleus and the cytoplasm. " O60934,"PROTEIN NAMES: Nibrin (Cell cycle regulatory protein p95) (Nijmegen breakage syndrome protein 1) (hNbs1) PROTEIN FAMILY: Nibrin family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the Nibrin family. FUNCTION: Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The MRN complex is involved in the repair of DNA double-strand breaks (DSBs) via homologous recombination (HR), an error-free mechanism which primarily occurs during S and G2 phases. The complex (1) mediates the end resection of damaged DNA, which generates proper single-stranded DNA, a key initial steps in HR, and is (2) required for the recruitment of other repair factors and efficient activation of ATM and ATR upon DNA damage. The MRN complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11, to initiate end resection, which is required for single-strand invasion and recombination. Within the MRN complex, NBN acts as a protein-protein adapter, which specifically recognizes and binds phosphorylated proteins, promoting their recruitment to DNA damage sites. Recruits MRE11 and RAD50 components of the MRN complex to DSBs in response to DNA damage. Promotes the recruitment of PI3/PI4-kinase family members ATM, ATR, and probably DNA-PKcs to the DNA damage sites, activating their functions. Mediates the recruitment of phosphorylated RBBP8/CtIP to DSBs, leading to cooperation between the MRN complex and RBBP8/CtIP to initiate end resection. RBBP8/CtIP specifically promotes the endonuclease activity of the MRN complex to clear DNA ends containing protein adducts. The MRN complex is also required for the processing of R-loops. NBN also functions in telomere length maintenance via its interaction with TERF2: interaction with TERF2 during G1 phase preventing recruitment of DCLRE1B/Apollo to telomeres. NBN also promotes DNA repair choice at dysfunctional telomeres: NBN phosphorylation by CK2 promotes non-homologous end joining repair at telomeres, while unphosphorylated NBN promotes microhomology-mediated end-joining (MMEJ) repair. Enhances AKT1 phosphorylation possibly by association with the mTORC2 complex. KEYWORDS: 3D-structure;Cell cycle;Chromosome;Direct protein sequencing;Disease variant;DNA damage;DNA repair;Host-virus interaction;Isopeptide bond;Meiosis;Nucleus;Phosphoprotein;Reference proteome;Telomere;Ubl conjugation SUBCELLULAR LOCATION: Nucleus Chromosome Nucleus, PML body Chromosome, telomere Note=Localizes to discrete nuclear foci after treatment with genotoxic agents. Localizes to DNA double-strand breaks (DSBs); recruited to DNA damage sites via association with phosphorylated proteins, such as phosphorylated H2AX, phosphorylated MDC1 and phosphorylated RAD17. Acetylation of 'Lys-5' of histone H2AX (H2AXK5ac) promotes NBN/NBS1 assembly at the sites of DNA damage. " O61267,"PROTEIN NAMES: Ovarian-specific serine/threonine-protein kinase Lok (Protein loki) (dMNK) PROTEIN FAMILY: Protein kinase superfamily, CAMK Ser/Thr protein kinase family, CDS1 subfamily ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CDS1 subfamily. FUNCTION: May have a role in germline establishment. KEYWORDS: Alternative splicing;ATP-binding;Kinase;Nucleotide-binding;Nucleus;Reference proteome;Serine/threonine-protein kinase;Transferase SUBCELLULAR LOCATION: Nucleus speckle Note=Speckled subnuclear compartment. " O61715,"PROTEIN NAMES: Innexin-19 (Neuronal symmetry protein 5) (Protein opu-19) PROTEIN FAMILY: Pannexin family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the pannexin family. FUNCTION: Structural component of the gap junctions that specifically coordinates left-right asymmetry in the developing nervous system. Acts by forming gap junction network linking embryonic neurons and providing electrical coupling between cells, leading to promote or inhibit AWC signaling. Required for the left and right AWC olfactory neurons to establish asymmetric patterns of gene expression during embryogenesis. Acts autonomously. KEYWORDS: Alternative splicing;Cell junction;Cell membrane;Developmental protein;Differentiation;Gap junction;Ion channel;Ion transport;Membrane;Neurogenesis;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Cell junction, gap junction Note=Specifically present at the transient gap junctions formed between the cell bodies of embryonic neurons. Excluded from axons and dendrites. " O62247,PROTEIN NAMES: Kunitz-type protein bli-5 (Blistered cuticle protein 5) (Kunitz-type protease inhibitor bli-5) ORGANISM: Caenorhabditis elegans FUNCTION: Appears to lack serine protease inhibitor activity in vitro when tested with bovine pancreatic alpha-chymotrypsin and elastase. Involved in cuticle biosynthesis. KEYWORDS: Disulfide bond;Reference proteome;Signal O62305,"PROTEIN NAMES: Calcium/calmodulin-dependent protein kinase type II (CaM kinase II) (Uncoordinated protein 43) PROTEIN FAMILY: Protein kinase superfamily, CAMK Ser/Thr protein kinase family, CaMK subfamily ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily. FUNCTION: Acts in the signaling of a variety of pathways and processes. Phosphorylates 'Ser-319' of daf-16 in response to stress signals, such as heat, starvation and oxidation, which plays a role in prolonging lifespan. Required for viability under chronic osmotic stress in which it acts downstream of osr-1. Has roles in locomotion, oocyte maturation, brood size, egg laying, defecation, meiotic maturation and neuronal cell fate specification. Required for the regulation of synaptic density and neuromuscular junction morphology. Regulates the synaptic trafficking of glr-1. Bidirectional modulator of neurotransmitter release with negative modulatory effects mainly mediated via slo-1 activation. Involved in activation of ADF neurons and increased tph-1 transcription following exposure to pathogenic bacteria which leads to learned olfactory aversion to the bacteria. Implicated in the muscle regulation of spicule protraction. In conjunction with egl-2 has a role in the suppression of mating behavior under food deprivation to encourage foraging. Involved in restricting str-2 expression to only one of the two AWC neurons. May suppress the functional response to an internal pacemaker, perhaps by modulating the activity of the IP3 receptor. KEYWORDS: 3D-structure;Alternative splicing;ATP-binding;Calmodulin-binding;Cell projection;Cytoplasm;Kinase;Magnesium;Metal-binding;Nucleotide-binding;Phosphoprotein;Reference proteome;Serine/threonine-protein kinase;Stress response;Transferase SUBCELLULAR LOCATION: Cytoplasm Cell projection, axon Perikaryon Note=Localizes at or near the Golgi apparatus. Localizes to post-synaptic regions and is enriched in punctate structures in axons of AWC neurons where it co-localizes with tir-1. Localization is regulated by tir-1. " O62415,PROTEIN NAMES: Lysozyme-like protein 1 PROTEIN FAMILY: Glycosyl hydrolase 25 family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the glycosyl hydrolase 25 family. FUNCTION: Involved in resistance to Gram-negative bacterium S.marcescens and to bacterium Gram-positive S.aureus infection. KEYWORDS: Antimicrobial;Cytoplasmic vesicle;Immunity;Innate immunity;Reference proteome;Signal SUBCELLULAR LOCATION: Cytoplasmic vesicle lumen Note=Localizes in vesicles in the apical region of intestinal cells. O62651,"PROTEIN NAMES: Wilms tumor protein homolog PROTEIN FAMILY: EGR C2H2-type zinc-finger protein family ORGANISM: Sus scrofa (Pig) SIMILARITY: Belongs to the EGR C2H2-type zinc-finger protein family. FUNCTION: Transcription factor that plays an important role in cellular development and cell survival. Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3'. Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors. Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing. Isoform 1 has lower affinity for DNA, and can bind RNA. KEYWORDS: Alternative splicing;Cytoplasm;DNA-binding;Isopeptide bond;Metal-binding;Nucleus;Reference proteome;Repeat;RNA editing;RNA-binding;Transcription;Transcription regulation;Tumor suppressor;Ubl conjugation;Zinc;Zinc-finger SUBCELLULAR LOCATION: [Isoform 1]: Nucleus speckle.; SUBCELLULAR LOCATION: [Isoform 4]: Nucleus, nucleoplasm.; SUBCELLULAR LOCATION: Nucleus Nucleus, nucleolus Cytoplasm Nucleus speckle Note=Shuttles between nucleus and cytoplasm. MISCELLANEOUS: Presence of the KTS motif hinders interactions between DNA and zinc-finger 4." O62830,"PROTEIN NAMES: Protein phosphatase 1B (Protein phosphatase 2C isoform beta) (PP2C-beta) PROTEIN FAMILY: PP2C family ORGANISM: Bos taurus (Bovine) SIMILARITY: Belongs to the PP2C family. FUNCTION: Enzyme with a broad specificity. Dephosphorylates PRKAA1 and PRKAA2. Inhibits TBK1-mediated antiviral signaling by dephosphorylating it at 'Ser-172'. Plays an important role in the termination of TNF-alpha-mediated NF-kappa-B activation through dephosphorylating and inactivating IKBKB/IKKB (By similarity). KEYWORDS: Alternative splicing;Cytoplasm;Hydrolase;Isopeptide bond;Lipoprotein;Magnesium;Manganese;Membrane;Metal-binding;Myristate;Phosphoprotein;Protein phosphatase;Reference proteome;Ubl conjugation SUBCELLULAR LOCATION: Cytoplasm, cytosol Membrane ; Lipid-anchor Note=Weakly associates at the membrane and N-myristoylation mediates the membrane localization. " O64399,PROTEIN NAMES: Transcription factor MYB34 (Myb-related protein 34) (AtMYB34) (Protein ALTERED TRYPTOPHAN REGULATION 1) (ATR1) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Transcription factor involved in tryptophan gene activation and in indole-3-acetic acid (IAA) and indolic glucosinolates (IG) biosynthesis. Acts as a direct transcriptional activator of both Trp synthesis genes and Trp secondary metabolism genes. KEYWORDS: Activator;DNA-binding;Nucleus;Reference proteome;Repeat;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus O64470,"PROTEIN NAMES: Spermidine hydroxycinnamoyl transferase (AtSHT) (BAHD-like hydroxycinnamoyl transferase) PROTEIN FAMILY: Plant acyltransferase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the plant acyltransferase family. FUNCTION: Hydroxycinnamoyl transferase involved in the conjugation of feruloyl CoA to spermidine. Catalyzes the three conjugating steps required for the biosynthesis of N(1),N(4),N(8)-triferuloyl-spermidine. Spermidine is the only acceptor substrate while feruloyl CoA > caffeoyl CoA > coumaroyl CoA > cinnamoyl CoA >> sinapoyl CoA are efficient acyl donors. No activity with hydroxyferuloyl CoA. Required for the biosynthesis of these conjugated spermidine derivatives, specifically in anther tapetum. KEYWORDS: 3D-structure;Acyltransferase;Reference proteome;Transferase " O64495,"PROTEIN NAMES: Subtilisin-like protease SBT1.2 (Cucumisin-like serine protease SDD1) (Protein STOMATAL DENSITY AND DISTRIBUTION 1) (Subtilase subfamily 1 member 2) (AtSBT1.2) (Subtilisin-like protease SDD1) PROTEIN FAMILY: Peptidase S8 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the peptidase S8 family. FUNCTION: Serine protease involved in the negative regulation of stomatal density and distribution. Not active on EPFL6 (AC Q1PEY6). Positive regulator of water use efficiency (WUE). KEYWORDS: Apoplast;Cell membrane;Glycoprotein;Hydrolase;Membrane;Protease;Reference proteome;Secreted;Serine protease;Signal SUBCELLULAR LOCATION: Secreted, extracellular space, apoplast Cell membrane ; Peripheral membrane protein " O64517,"PROTEIN NAMES: Metacaspase-4 (AtMC4) (Metacaspase 2d) (AtMCP2d) (Metacaspase-7) [Cleaved into: Metacaspase-4 subunit p20; Metacaspase-4 subunit p10] PROTEIN FAMILY: Peptidase C14B family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the peptidase C14B family. FUNCTION: Cysteine protease that cleaves specifically after arginine or lysine residues. Does not cleave caspase-specific substrates. Plays a positive regulatory role in biotic and abiotic stress-induced programmed cell death. KEYWORDS: 3D-structure;Autocatalytic cleavage;Cytoplasm;Hydrolase;Plant defense;Protease;Reference proteome;S-nitrosylation;Thiol protease SUBCELLULAR LOCATION: Cytoplasm, cytosol MISCELLANEOUS: Plants overexpressing MCA4 are more sensitive to the mycotoxin fumonisin B1 and methyl viologen (oxidative stress) and exhibited accelerated cell-death progression." O64587,"PROTEIN NAMES: Accelerated cell death 11 (Ceramide-1-phosphate transfer protein ACD11) (Glycolipid transfer protein domain-containing protein ACD11) PROTEIN FAMILY: GLTP family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the GLTP family. FUNCTION: Exhibits selective intermembrane transfer of ceramide-1-phosphate (C1P) and phytoceramide-1-phosphate. Does not transport ceramide (Cer) or GalCer, suggesting a requirement for phosphate in the headgroup for functionality. Transports in vitro sphingosine, but not glycosphingolipids. Has also some in vitro activity with sphingomyelin, a lipid not detected in plant tissues. The transport function may be not directly involved in regulating cell death. Rather, perturbations in the function of ACD11 or related components could be monitored by R-proteins, which then mediate defense and programmed cell death (PCD), as proposed in the guard hypothesis (Probable). C1P transfer is stimulated by phosphatidylserine in C1P source vesicles. Regulates autophagy, inflammasome mediated IL1B and IL18 processing, and pyroptosis, but not apoptosis. KEYWORDS: 3D-structure;Cytoplasm;Lipid transport;Lipid-binding;Reference proteome;Transport SUBCELLULAR LOCATION: Cytoplasm MISCELLANEOUS: The clustered Lys-64/Arg-99/Arg-103 residues form a positively charged triad ideally arranged for binding phosphate, explaining the inability to bind sugar headgroups and transfer glycoproteins." O64883,"PROTEIN NAMES: Beta-glucosidase 26, peroxisomal (AtBGLU26) (Protein PENETRATION 2) PROTEIN FAMILY: Glycosyl hydrolase 1 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the glycosyl hydrolase 1 family. FUNCTION: Possesses beta-glucosidase activity toward 4-methyl-umbelliferyl-beta-D-glucoside in vitro. Possesses myrosinase activity toward indol-3-yl-methylglucosinolate (I3M) and 4-methoxy-indol-3-yl-methylglucosinolate (4MO-I3M) in vivo. Component of an inducible preinvasion resistance mechanism that prevents penetration of the nonhost fungal species B.graminis and E.pisi. Involved in indole glucosinolate (IGS) activation during pattern-triggered immunity (PTI). Functions as a myrosinase for the breakdown of flg22-triggered IGS. Required for both callose deposition and glucosinolate activation during pathogen-triggered resistance. During fungal attack, required for IGS activation that mediates broad-spectrum antifungal defense. KEYWORDS: Glycosidase;Hydrolase;Peroxisome;Plant defense;Reference proteome SUBCELLULAR LOCATION: Peroxisome " O64948,"PROTEIN NAMES: Lon protease homolog 2, peroxisomal PROTEIN FAMILY: Peptidase S16 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the peptidase S16 family. FUNCTION: ATP-dependent serine protease that mediates the selective degradation of misfolded and unassembled polypeptides in the peroxisomal matrix (By similarity). Necessary for type 2 peroxisome targeting signal (PTS2)-containing protein processing and facilitates peroxisome matrix protein import. KEYWORDS: ATP-binding;Hydrolase;Nucleotide-binding;Peroxisome;Protease;Reference proteome;Serine protease SUBCELLULAR LOCATION: Peroxisome matrix " O65502,"PROTEIN NAMES: Protein HIGH CHLOROPHYLL FLUORESCENCE PHENOTYPE 244, chloroplastic PROTEIN FAMILY: NmrA-type oxidoreductase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the NmrA-type oxidoreductase family. FUNCTION: Auxiliary factor required, together with HCF173, for the biogenesis of photosystem II (PSII), especially for the synthesis of the reaction center proteins (e.g. D1), via the regulation of the corresponding mRNA (e.g. psbA) translation initiation (ribosomal loading) and stabilization. Forms a trimeric complex with OHP1 and OHP2 that is required to promote PSII core subunit assembly. The trimeric complex forms a transient PSII reaction center-like complex with PsbA, PsbD, PsbE, PsbF and PsbI subunits in thylakoids for early assembly of PSII as well as PSII repair. The trimeric complex is required for the recruitment of ribosomes to the psbA mRNA during PSII biogenesis and repair. KEYWORDS: Chloroplast;Membrane;Photosynthesis;Photosystem II;Plastid;Reference proteome;Thylakoid;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast stroma Plastid, chloroplast thylakoid membrane ; Peripheral membrane protein ; Stromal side Note=Predominantly present at thylakoid membranes. " O65508,"PROTEIN NAMES: NAC domain-containing protein 76 (ANAC076) (Protein VASCULAR RELATED NAC-DOMAIN 2) PROTEIN FAMILY: Plant vascular related NAC-domain protein family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the plant vascular related NAC-domain protein family. FUNCTION: Transcription activator that binds to the secondary wall NAC binding element (SNBE), 5'-(T/A)NN(C/T)(T/C/G)TNNNNNNNA(A/C)GN(A/C/T)(A/T)-3', in the promoter of target genes (By similarity). Involved in xylem formation by promoting the expression of secondary wall-associated transcription factors and of genes involved in secondary wall biosynthesis and programmed cell death, genes driven by the secondary wall NAC binding element (SNBE). Triggers thickening of secondary walls. KEYWORDS: Activator;Cell wall biogenesis/degradation;Developmental protein;DNA-binding;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " O65570,"PROTEIN NAMES: Villin-4 PROTEIN FAMILY: Villin/gelsolin family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the villin/gelsolin family. FUNCTION: Binds actin and actin filament bundles in a Ca(2+)-insensitive manner, but caps the barbed end of actin filaments and is able to sever them in a calcium-dependent manner. Involved in root hair growth through regulating actin organization in a Ca(2+)-dependent manner. KEYWORDS: 3D-structure;Actin capping;Actin-binding;Alternative splicing;Calcium;Cytoplasm;Cytoskeleton;Phosphoprotein;Reference proteome;Repeat SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton " O65718,"PROTEIN NAMES: Cyclic nucleotide-gated ion channel 2 (AtCNGC2) (Cyclic nucleotide- and calmodulin-regulated ion channel 2) (Protein DEFENSE NO DEATH 1) PROTEIN FAMILY: Cyclic nucleotide-gated cation channel family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the cyclic nucleotide-gated cation channel (TC 1.A.1.5) family. FUNCTION: Acts as a cyclic nucleotide-gated ion channel. Permeable to potassium and calcium in a cyclic nucleotide-dependent fashion (cAMP or cGMP). Could also transport lithium, cesium and rubium and displays a strong selectivity against sodium. Seems to directly participate in pathogen-induced calcium influx. May function in homeostasis, re-establishing ionic balance after defense action and/or other stimuli. Could mediate the initiation of the developmentally regulated cell death programs. KEYWORDS: Alternative splicing;Calmodulin-binding;cAMP;cAMP-binding;Cell membrane;cGMP;cGMP-binding;Ion channel;Ion transport;Ligand-gated ion channel;Membrane;Nucleotide-binding;Plant defense;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein MISCELLANEOUS: Loss-of-function mutations cngc2-1 (dnd1-1) or cncg2-2 results in the loss of the hypersensitive response and leads to a broad spectrum disease resistance. These mutations lead to a specific and dramatic calcium hypersensitivity that results in severe reductions in plant size and seed yield." A0A1B0GTW7,PROTEIN NAMES: Ciliated left-right organizer metallopeptidase (Leishmanolysin-like peptidase 2) PROTEIN FAMILY: Peptidase M8 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the peptidase M8 family. FUNCTION: Putative metalloproteinase that plays a role in left-right patterning process. KEYWORDS: Alternative splicing;Disease variant;Glycoprotein;Heterotaxy;Hydrolase;Membrane;Metal-binding;Metalloprotease;Protease;Reference proteome;Signal;Transmembrane;Transmembrane helix;Zinc SUBCELLULAR LOCATION: Membrane ; Single-pass type I membrane protein A0A1B1J8Z2,"PROTEIN NAMES: Class V chitinase CHIT5 (LjCHIT5) PROTEIN FAMILY: Glycosyl hydrolase 18 family, Chitinase class V subfamily ORGANISM: Lotus japonicus (Lotus corniculatus var. japonicus) SIMILARITY: Belongs to the glycosyl hydrolase 18 family. Chitinase class V subfamily. FUNCTION: Possesses chitinase activity in vitro toward glycol chitin, carboxymethyl-chitin, colloidal chitin, and the chitin oligosaccharides (N-acetylglucosamine) (GlcNAc)6 and (GlcNAc)5. Hydrolyzes (GlcNAc)6 into (GlcNAc)4 and (GlcNAc)2, or two (GlcNAc)3 molecules. Has the capacity to inhibit hyphal growth of the fungus Trichoderma viride in an agar-plate bioassay. Involved in symbiotic signaling. Required for root hair infection threads (ITs) elongation and nodule development. Possesses Nod factor (NF) hydrolase activity. NFs are lipo-chitooligosaccharide signaling molecules produced by nitrogen-fixing rhizobia to initiate nodulation (symbiosis) on the roots of legumes. Modulates NF levels and signaling to complete transition of infected nodules to functional nitrogen-fixing organs. PATHWAY: Glycan degradation; chitin degradation. KEYWORDS: Carbohydrate metabolism;Chitin degradation;Glycoprotein;Glycosidase;Hydrolase;Nodulation;Plant defense;Polysaccharide degradation;Signal " A0A1B4XBK0,"PROTEIN NAMES: Geranylgeranyl diphosphate synthase sdnC ((2E,6E)-farnesyl diphosphate synthase) (Dimethylallyltranstransferase) (Farnesyl diphosphate synthase) (Farnesyltranstransferase) (Geranylgeranyl pyrophosphate synthase) (GGPP synthase) (GGPPSase) (Geranyltranstransferase) (Sordarin/hypoxysordarin biosynthesis cluster protein C) PROTEIN FAMILY: FPP/GGPP synthase family ORGANISM: Sordaria araneosa (Pleurage araneosa) SIMILARITY: Belongs to the FPP/GGPP synthase family. FUNCTION: Geranylgeranyl diphosphate synthase; part of the gene cluster that mediates the biosynthesis of sordarin and hypoxysordarin, glycoside antibiotics with a unique tetracyclic diterpene aglycone structure. First, the geranylgeranyl diphosphate synthase sdnC constructs GGDP from farnesyl diphosphate and isopentenyl diphosphate. The diterpene cyclase sdnA then catalyzes the cyclization of GGDP to afford cycloaraneosene. Cycloaraneosene is then hydroxylated four times by the putative cytochrome P450 monooxygenases sdnB, sdnE, sdnF and sdnH to give a hydroxylated cycloaraneosene derivative such as cycloaraneosene-8,9,13,19-tetraol. Although the order of the hydroxylations is unclear, at least C8, C9 and C13 of the cycloaraneosene skeleton are hydroxylated before the sordaricin formation. Dehydration of the 13-hydroxy group of the hydroxylated cycloaraneosene derivative might be catalyzed by an unassigned hypothetical protein such as sdnG and sdnP to construct the cyclopentadiene moiety. The FAD-dependent oxidoreductase sdnN is proposed to catalyze the oxidation at C9 of the hydroxylated cycloaraneosene derivative and also catalyze the Baeyer-Villiger oxidation to give the lactone intermediate. The presumed lactone intermediate would be hydrolyzed to give an acrolein moiety and a carboxylate moiety. Then, [4+2]cycloaddition would occur between the acrolein moiety and the cyclopentadiene moiety to give sordaricin. SdnN might also be involved in the [4+2]cycloaddition after the hypothesized oxidation to accommodate the oxidized product and prompt the [4+2]cycloaddition. GDP-6-deoxy-D-altrose may be biosynthesized from GDP-D-mannose by the putative GDP-mannose-4,6-dehydratase sdnI and the short-chain dehydrogenase sdnK. The glycosyltransferase sdnJ catalyzes the attachment of 6-deoxy-D-altrose onto the 19-hydroxy group of sordaricin to give 4'-O-demethylsordarin. The methyltransferase sdnD would complete the biosynthesis of sordarin. Sordarin can be further modified into hypoxysordarin. The unique acyl chain at the 3'-hydroxy group of hypoxysordarin would be constructed by an iterative type I PKS sdnO and the trans-acting polyketide methyltransferase sdnL. SdnL would be responsible for the introduction of an alpha-methyl group of the polyketide chain. Alternatively, the beta-lactamase-like protein sdnR might be responsible for the cleavage and transfer of the polyketide chain from the PKS sdnO to sordarin. Two putative cytochrome P450 monooxygenases, sdnQ and sdnT, might catalyze the epoxidations of the polyketide chain to complete the biosynthesis of hypoxysordarin. Transcriptional regulators sdnM and sdnS are presumably encoded for the transcriptional regulation of the expression of the sdn gene cluster. PATHWAY: Antibiotic biosynthesis. KEYWORDS: Antibiotic biosynthesis;Isoprene biosynthesis;Magnesium;Metal-binding;Transferase " A0A1D5NS60,"PROTEIN NAMES: Zinc finger protein 16-like (Zinc finger protein 697-like) PROTEIN FAMILY: Krueppel C2H2-type zinc-finger protein family ORGANISM: Danio rerio (Zebrafish) (Brachydanio rerio) SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein family. FUNCTION: Probable transcription factor (Probable). Important for development and migration of oligodendrocyte precursor cells, and normal myelination of axons in the central nervous system (CNS). Functions autonomously in oligodendrocytes to promote CNS myelination. Seems to act in parallel with notch3 during oligodendrocyte development. KEYWORDS: Alternative splicing;Coiled coil;Metal-binding;Neurogenesis;Nucleus;Reference proteome;Repeat;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus " A0A1E1FFL0,"PROTEIN NAMES: Multifunctional dioxygenase prhA (Paraherquonin biosynthesis cluster protein A) PROTEIN FAMILY: PhyH family ORGANISM: Penicillium brasilianum SIMILARITY: Belongs to the PhyH family. FUNCTION: Multifunctional dioxygenase; part of the gene cluster that mediates the biosynthesis of paraherquonin, a meroterpenoid with a unique, highly congested hexacyclic molecular architecture. The first step of the pathway is the synthesis of 3,5-dimethylorsellinic acid (DMOA) by the polyketide synthase prhL (By similarity). Synthesis of DMOA is followed by farnesylation by the prenyltransferase prhE, methylesterification by the methyl-transferase prhM, epoxidation of the prenyl chain by the flavin-dependent monooxygenase prhF, and cyclization of the farnesyl moiety by the terpene cyclase prhH, to yield the tetracyclic intermediate, protoaustinoid A (By similarity). The short chain dehydrogenase prhI then oxidizes the C-3 alcohol group of the terpene cyclase product to transform protoaustinoid A into protoaustinoid B. The FAD-binding monooxygenase prhJ catalyzes the oxidation of protoaustinoid B into preaustinoid A which is further oxidized into preaustinoid A1 by FAD-binding monooxygenase phrK. Finally, prhA leads to berkeleydione via the berkeleyone B intermediate. PrhA is a multifunctional dioxygenase that first desaturates at C5-C6 to form berkeleyone B, followed by rearrangement of the A/B-ring to form the cycloheptadiene moiety in berkeleydione. Berkeleydione serves as the key intermediate for the biosynthesis of paraherquonin as well as many other meroterpenoids (Probable). The cytochrome P450 monooxygenases prhB, prhD, and prhN, as well as the isomerase prhC, are probably involved in the late stage of paraherquonin biosynthesis, after the production of berkeleydione (Probable). Especially prhC might be a multifunctional enzyme that catalyzes the D-ring expansion via intramolecular methoxy rearrangement, as well as the hydrolysis of the expanded D-ring (Probable). PATHWAY: Secondary metabolite biosynthesis; terpenoid biosynthesis. KEYWORDS: 3D-structure;Dioxygenase;Iron;Metal-binding;Oxidoreductase " A0A1J1DL12,"PROTEIN NAMES: 2-iminobutanoate/2-iminopropanoate deaminase (Allergen Der f 34) (Enamine/imine deaminase) (allergen Der f 34.0101) PROTEIN FAMILY: RutC family ORGANISM: Dermatophagoides farinae (American house dust mite) SIMILARITY: Belongs to the RutC family. FUNCTION: Catalyzes the hydrolytic deamination of enamine/imine intermediates that form during the course of normal metabolism. May facilitate the release of ammonia from these potentially toxic reactive metabolites, reducing their impact on cellular components. It may act on enamine/imine intermediates formed by several types of pyridoxal-5'-phosphate-dependent dehydratases including L-threonine dehydratase (By similarity). Preferentially digests Leu and Met in cooperation with L-amino acid oxidase, but digests Phe poorly. KEYWORDS: Allergen;Cytoplasm;Direct protein sequencing;Hydrolase SUBCELLULAR LOCATION: Cytoplasm " A0A1L4BJ46,"PROTEIN NAMES: Phospholipase A2 hemilipin (Phosphatidylcholine 2-acylhydrolase) (Phospholipase A(2)) [Cleaved into: Phospholipase A2 large subunit; Phospholipase A2 small subunit] PROTEIN FAMILY: Phospholipase A2 family, Group III subfamily ORGANISM: Hemiscorpius lepturus (Scorpion) SIMILARITY: Belongs to the phospholipase A2 family. Group III subfamily. FUNCTION: Scorpion venom phospholipase A2 (PLA2) that shows high hydrolytic activities towards lecithin and acts as an effective blocker of all angiogenesis key steps in vivo and in vitro. It has no effect on apoptosis and does not display hemolytic, inflammatory or neurotoxic effects. PLA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. KEYWORDS: Calcium;Cleavage on pair of basic residues;Direct protein sequencing;Disulfide bond;Glycoprotein;Hydrolase;Lipid degradation;Lipid metabolism;Metal-binding;Secreted;Signal;Zymogen SUBCELLULAR LOCATION: Secreted " A0A1L6Z3A0,"PROTEIN NAMES: Pseudolaratriene synthase, chloroplastic (Terpene synthase 8) (PxaTPS8) PROTEIN FAMILY: Terpene synthase family ORGANISM: Pseudolarix amabilis (Golden larch) (Pseudolarix kaempferi) SIMILARITY: Belongs to the terpene synthase family. FUNCTION: Converts geranylgeranyl diphosphate to an new 5,7-fused bicyclic diterpene, named pseudolaratriene. Catalyzes the first committed step in pseudolaric acid B (PAB) biosynthesis. PAB exhibits antiproliferative activity by inhibiting microtubule polymerization, and has demonstrated antitumor properties against several cancer types (Probable). PATHWAY: Terpene metabolism. KEYWORDS: Chloroplast;Lyase;Magnesium;Metal-binding;Plastid;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast " A0A1L8G2K9,"PROTEIN NAMES: DNA-dependent metalloprotease SPRTN (Protein with SprT-like domain at the N terminus) (Spartan) PROTEIN FAMILY: Spartan family ORGANISM: Xenopus laevis (African clawed frog) SIMILARITY: Belongs to the Spartan family. FUNCTION: DNA-dependent metalloendopeptidase that mediates the proteolytic cleavage of covalent DNA-protein cross-links (DPCs) during DNA synthesis, thereby playing a key role in maintaining genomic integrity. DPCs are highly toxic DNA lesions that interfere with essential chromatin transactions, such as replication and transcription, and which are induced by reactive agents, such as UV light or formaldehyde (By similarity). Associates with the DNA replication machinery and specifically removes DPCs during DNA synthesis (By similarity). Catalyzes proteolytic cleavage of the hmces DNA-protein cross-link following unfolding by the brip1/fancj helicase. Acts as a pleiotropic protease for DNA-binding proteins cross-linked with DNA, such as top1, top2a, histones H3 and H4 (By similarity). Mediates degradation of DPCs that are not ubiquitinated, while it is not able to degrade ubiquitinated DPCs. SPRTN activation requires polymerase collision with DPCs followed by helicase bypass of DPCs. May also act as a 'reader' of ubiquitinated pcna: facilitates chromatin association of rad18 and is required for efficient pcna monoubiquitination, promoting a feed-forward loop to enhance pcna ubiquitination and translesion DNA synthesis (By similarity). Acts as a regulator of translesion DNA synthesis by recruiting vcp/p97 to sites of DNA damage (By similarity). KEYWORDS: Autocatalytic cleavage;Chromosome;DNA damage;DNA repair;Hydrolase;Isopeptide bond;Metal-binding;Metalloprotease;Nucleus;Protease;Reference proteome;Repeat;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Chromosome Note=Localizes to sites of UV damage via the PIP-box (By similarity). Recruited to stalled replication forks at sites of replication stress (By similarity). " A0A1L8HU22,"PROTEIN NAMES: Endonuclease 8-like 3 (DNA glycosylase/AP lyase Neil3) (Endonuclease VIII-like 3) (Nei-like protein 3) PROTEIN FAMILY: FPG family ORGANISM: Xenopus laevis (African clawed frog) SIMILARITY: Belongs to the FPG family. FUNCTION: DNA glycosylase which prefers single-stranded DNA (ssDNA), or partially ssDNA structures such as bubble and fork structures, to double-stranded DNA (dsDNA). Mediates interstrand cross-link repair in response to replication stress: recruited to replication stress sites via interaction with ubiquitinated CMG helicase and acts by mediating DNA glycosylase activity. Cleaves one of the two N-glycosyl bonds comprising the interstrand cross-link, which avoids the formation of a double-strand break but generates an abasic site that is bypassed by translesion synthesis polymerases. KEYWORDS: Chromosome;DNA damage;DNA repair;DNA-binding;Glycosidase;Hydrolase;Lyase;Metal-binding;Multifunctional enzyme;Nucleus;Reference proteome;Repeat;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Chromosome Note=Recruited to replication stress sites via interaction with ubiquitinated CMG helicase. " A0A1S6M251,"PROTEIN NAMES: Beta-1,4-galactosyltransferase 5 (Beta-1,4-GalTase 5) (Beta4Gal-T5) (b4Gal-T5) (Beta-1,4-GalT II) (Glucosylceramide beta-1,4-galactosyltransferase) (Lactosylceramide synthase) (LacCer synthase) (UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 5) (UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 5) PROTEIN FAMILY: Glycosyltransferase 7 family ORGANISM: Sus scrofa (Pig) SIMILARITY: Belongs to the glycosyltransferase 7 family. FUNCTION: Catalyzes the synthesis of lactosylceramide (LacCer) via the transfer of galactose from UDP-galactose to glucosylceramide (GlcCer) (By similarity). LacCer is the starting point in the biosynthesis of all gangliosides (membrane-bound glycosphingolipids) which play pivotal roles in the CNS including neuronal maturation and axonal and myelin formation (By similarity). Plays a role in the glycosylation of BMPR1A and regulation of its protein stability (By similarity). Essential for extraembryonic development during early embryogenesis (By similarity).; FUNCTION: (Microbial infection) May play a role in the glycosylation of porcine reproductive and respiratory syndrome virus GP5 protein and may be involved in the regulation of viral proliferation. PATHWAY: Protein modification; protein glycosylation.; PATHWAY: Sphingolipid metabolism. KEYWORDS: Disulfide bond;Glycoprotein;Glycosyltransferase;Golgi apparatus;Lipid biosynthesis;Lipid metabolism;Manganese;Membrane;Metal-binding;Reference proteome;Signal-anchor;Sphingolipid metabolism;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus, Golgi stack membrane ; Single-pass type II membrane protein. Golgi apparatus Note=Trans cisternae of Golgi stack. " A0A1V0QSA8,"PROTEIN NAMES: Geranylgeranyl pyrophosphate synthase AN1592 (GGPP synthase) (GGPPSase) ((2E,6E)-farnesyl diphosphate synthase) (Dimethylallyltranstransferase) (Erinacine biosynthesis cluster protein E) (Farnesyl diphosphate synthase) (Farnesyltranstransferase) (Geranylgeranyl diphosphate synthase) (Geranyltranstransferase) PROTEIN FAMILY: FPP/GGPP synthase family ORGANISM: Hericium erinaceus (Lion's mane mushroom) (Hydnum erinaceus) SIMILARITY: Belongs to the FPP/GGPP synthase family. FUNCTION: Geranylgeranyl pyrophosphate synthase; part of the gene cluster that mediates the biosynthesis of erinacines, cyathane-xylosides that show unique biological activities, including leishmanicidal activity, stimulating activity for nerve growth-factor synthesis, and agonistic activity toward the kappa opioid receptor. The geranylgeranyl diphosphate (GGPP) synthase eriE catalyzes the first step in erinacines biosynthesis via conversion of farnesyl pyrophosphate and isopentyl pyrophosphate into geranylgeranyl pyrophosphate (GGPP) (By similarity). GGPP is then substrate of the diterpene cyclase eriG for the production of cyatha-3,12-diene. The cytochrome P450 monooxygenase eriI then hydroxylates cyatha-3,12-diene at C-14 of the seven-membered ring to produce erinacol, which is further hydroxylated at C-15 by the cytochrome P450 monooxygenase eriC to yield cyathadiol. The cytochrome P450 monooxygenase eriA then catalyzes C-11 hydroxylation in the presence of the short chain dehydrogenase/reductase (SDR) eriH, which leads to the production of cyathatriol. The acetyltransferase eriL converts cyathatriol into 11-O-acetyl-cyathatriol. The SDR eriH catalyzes further oxidation of 11-O-acetyl-cyathatriol into 1-O-acetylcyathin A3. Finally, the glycosyl transferase eriJ tranfers xylose from UDP-xylose onto C-14 of 11-O-acetyl-cyathatriol to form eracine Q. EriJ is also able to convert 11-O-acetyl-cyathatriol to eracine Q2 by using UDP-D-glucose as cosubstrate, but at a lower rate. In the absence of eriL and eriJ, the SDR eriH is able to convert cyathatriol to cyathin A3; this is likely a switching mechanism in the biosynthesis of cyathins (C-14 ketogroup)and erinacines (C-14 glycosylated group). The roles of the SDR eriB, the polyprenyl transferase eriF and the dehydrogenase eriK have still to be identified (Probable). PATHWAY: Secondary metabolite biosynthesis. KEYWORDS: Isoprene biosynthesis;Magnesium;Metal-binding;Transferase " Q8GAK6,"PROTEIN NAMES: D-xylose dehydrogenase (NADP-dependent D-xylose dehydrogenase) PROTEIN FAMILY: Gfo/Idh/MocA family ORGANISM: Paenarthrobacter nicotinovorans (Arthrobacter nicotinovorans) SIMILARITY: Belongs to the Gfo/Idh/MocA family. FUNCTION: Catalyzes the NADP(+)-dependent oxidation of D-xylose. Is able to use both NADP(+) and NAD(+); however, the enzyme shows a very strong preference for NADP(+). Is likely involved in the first step of the oxidative D-xylose degradation pathway. PATHWAY: Carbohydrate metabolism; D-xylose degradation. KEYWORDS: Carbohydrate metabolism;NAD;NADP;Oxidoreductase;Plasmid " Q8GAW0,"PROTEIN NAMES: Cyclopentanone 1,2-monooxygenase (CPMO) (Baeyer-Villiger monooxygenase) (BVMO) PROTEIN FAMILY: FAD-binding monooxygenase family ORGANISM: Comamonas sp. (strain NCIMB 9872) SIMILARITY: Belongs to the FAD-binding monooxygenase family. FUNCTION: Catalyzes a Baeyer-Villiger oxidation reaction, i.e. the insertion of an oxygen atom into a carbon-carbon bond adjacent to a carbonyl, which converts ketones to esters or lactones using NADPH as an electron donor. Converts cyclopentanone to 5-valerolactone, a step in the degradation pathway of cyclopentanol. Besides cycloalkanones, can also act on methylated and other alkylated cycloalkanones, and on methylated cycloalkenones, with high enantioselectivity in some cases. Cannot use NADH instead of NADPH. PATHWAY: Alcohol metabolism; cyclopentanol degradation; 5-valerolactone from cyclopentanol: step 2/2. KEYWORDS: Direct protein sequencing;FAD;Flavoprotein;Monooxygenase;NADP;Oxidoreductase " Q8GFE2,"PROTEIN NAMES: Chlorophenol reductase (Ortho-chlorophenol reductive dehalogenase) ORGANISM: Desulfitobacterium hafniense (Desulfitobacterium frappieri) FUNCTION: Reductive dechlorination of ortho-chlorophenols. Dechlorinates in the ortho position with respect to the hydroxyl group. KEYWORDS: Aromatic hydrocarbons catabolism;Cell membrane;Cell wall;Cobalamin;Cobalt;Direct protein sequencing;Membrane;Secreted;Signal SUBCELLULAR LOCATION: Secreted Secreted, cell wall Cell membrane Note=Associated with the cell membrane. Probably binds to peptidoglycans of the cell membrane and cell wall. " Q8GGD4,"PROTEIN NAMES: N(alpha)-acyl-glutamine aminoacylase (N-AGA) (Axillary malodor releasing enzyme) (AMRE) PROTEIN FAMILY: Peptidase M20 family ORGANISM: Corynebacterium striatum SIMILARITY: Belongs to the peptidase M20 family. FUNCTION: Hydrolyzes odorless N-alpha-acyl-L-glutamine conjugates of short- and medium-chain fatty acids, releasing human axillary malodor compounds. The enzyme is highly specific for the glutamine residue but has a low specificity for the acyl part of the substrate. The two most common products are 3-methyl-2-hexenoic acid (3M2H) and 3-hydroxy-3-methyl-hexanoic acid (HMHA), which are produced from the odorless precursors N-alpha-3-methyl-2-hexenoyl-L-glutamine (3M2H-Gln) and N-alpha-3-hydroxy-3-methylhexanoyl-L-glutamine (HMHA-Gln). In addition, over 28 different carboxylic acids contributing to human body odor are released by this enzyme from odorless axilla secretions, including several aliphatic 3-hydroxy acids with 4-Me branches, 3,4-unsaturated, 4-Et-branched aliphatic acids, and a variety of degradation products of amino acids. KEYWORDS: 3D-structure;Direct protein sequencing;Hydrolase " Q8GHB2,PROTEIN NAMES: 4-hydroxyphenylpyruvate 3-dimethylallyltransferase (4HPP 3-dimethylallyltransferase) (Aromatic prenyltransferase CloQ) (Clorobiocin biosynthesis protein Q) PROTEIN FAMILY: Aromatic prenyltransferase family ORGANISM: Streptomyces roseochromogenus subsp. oscitans SIMILARITY: Belongs to the aromatic prenyltransferase family. FUNCTION: Magnesium-independent aromatic prenyltransferase that catalyzes the irreversible transfer of a dimethylallyl group to 4-hydroxyphenylpyruvate to produce the ring A structure in the clorobiocin biosynthesis pathway. Clorobiocin is an aminocoumarin family antibiotic. PATHWAY: Antibiotic biosynthesis. KEYWORDS: 3D-structure;Antibiotic biosynthesis;Prenyltransferase;Transferase Q8GSQ1,"PROTEIN NAMES: Cytochrome P450 85A1 (OsCYP85A1) (3-dehydroteasterone synthase) (C6-oxidase) (Protein DWARF) (OsDWARF) (Teasterone synthase) (Typhasterol synthase) PROTEIN FAMILY: Cytochrome P450 family ORGANISM: Oryza sativa subsp. japonica (Rice) SIMILARITY: Belongs to the cytochrome P450 family. FUNCTION: Catalyzes the C6-oxidation step in brassinosteroids biosynthesis. May convert 6-deoxoteasterone (6-deoxoTE) to teasterone (TE), 3-dehydro-6-deoxoteasterone (6-deoxo3DT, 6-deoxo3DHT) to 3-dehydroteasterone (3DT, 3-DHT), and 6-deoxotyphasterol (6-deoxoTY) to typhasterol (TY). Involved in the organization and elongation of the leaf and stem cells. Not able to convert 6-deoxocastasterone (6-deoxoCS) and castasterone (CS) to brassinolide (BL). PATHWAY: Plant hormone biosynthesis; brassinosteroid biosynthesis. KEYWORDS: Brassinosteroid biosynthesis;Heme;Iron;Lipid biosynthesis;Lipid metabolism;Membrane;Metal-binding;Monooxygenase;Oxidoreductase;Reference proteome;Steroid biosynthesis;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Single-pass membrane protein " Q8GUE4,"PROTEIN NAMES: Geraniol synthase, chloroplastic (CtGES) PROTEIN FAMILY: Terpene synthase family, Tpsb subfamily ORGANISM: Cinnamomum tenuipile (Alseodaphne mollis) SIMILARITY: Belongs to the terpene synthase family. Tpsb subfamily. FUNCTION: Monoterpene synthase that catalyzes the formation of geraniol from geranyl diphosphate. PATHWAY: Secondary metabolite biosynthesis; terpenoid biosynthesis. KEYWORDS: Chloroplast;Hydrolase;Magnesium;Manganese;Metal-binding;Plastid;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast MISCELLANEOUS: Exclusively observed in the geraniol chemotype of this organism." Q8GUI6,"PROTEIN NAMES: Lysine-specific demethylase JMJ14 (Jumonji domain-containing protein 14) (AtJMJ14) (Protein JUMONJI 14) (Jumonji domain-containing protein 4) (AtJmj4) (Lysine-specific histone demethylase JMJ14) ([histone H3]-trimethyl-L-lysine(4) monodemethylase JMJ14) PROTEIN FAMILY: JARID1 histone demethylase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the JARID1 histone demethylase family. FUNCTION: Transcriptional repressor. Histone demethylase that demethylates 'Lys-4' (H3K4me) of histone H3 with a higher activity for H3K4me3 and H3K4me2 than H3K4me1. No activity on H3K9me3/2, H3K36me3/2 and H3K27me3/2. Function as a nocturne 'eraser' to counteract the diurnal 'writer' methylase activity of ATXR3/SDG2 thus orchestrating the circadian rythm of histone modifications (e.g. H3K4me3) and modulating the rythmic expression of diurnal target genes; this mechanism relies also on the circadian clock oscillators CCA1 and LHY. Involved in a negative regulation of root meristem growth upon suboptimal root growth conditions. Represses FT and TSF expression to inhibit the floral transition. Binds around the transcription start site of the FT locus. Involved in the DRM2-mediated maintenance of DNA methylation, but not required for the de novo DNA methylation. Required for demethylating histone H3K4me3 at the target of RNA silencing. Counteracts the DNA methylation of expressed transgenes; specific attenuation of transgene DNA methylation enhances the production of aberrant RNAs (e.g. uncapped and antisense) that readily induce systemic RDR6-dependent post-transcriptional transgene silencing (PTGS) spreading. Together with NAC051/NAC052 and NAC050, regulates gene expression and flowering time, probably by the promotion of RNA-mediated gene silencing. Together with JMJ16 and JMJ17, required for plant growth and development. Promotes local and systemic immunity (especially toward the bacterial pathogen Pseudomonas syringae Pst DC3000 avrRpt2) by regulating positively pathogen-induced H3K4me3 enrichment and expression of defense genes involved in salicylic acid (SA)- and pipecolic acid (Pip)-mediated defense pathways (e.g. PR1, FMO1, ALD1 and SARD4). KEYWORDS: 3D-structure;Alternative splicing;Chromatin regulator;Dioxygenase;Flowering;Hypersensitive response;Iron;Metal-binding;Nucleus;Oxidoreductase;Plant defense;Reference proteome;Repressor;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus, nucleoplasm Nucleus Note=Not detected in the nucleolus and the chromocenters. " Q8GUJ2,"PROTEIN NAMES: Plasmodesmata-located protein 5 (PD-located protein 5) (Cysteine-rich repeat protein HWI1) (Cysteine-rich repeat secretory protein 2) (Plasmodesmata localizing protein 5) (Protein HOPW1-1-INDUCED 1) PROTEIN FAMILY: Cysteine-rich repeat secretory protein family, Plasmodesmata-located proteins (PDLD) subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the cysteine-rich repeat secretory protein family. Plasmodesmata-located proteins (PDLD) subfamily. FUNCTION: Modulates cell-to-cell trafficking. Has a positive role in innate immunity. Required for systemic acquired resistance (SAR) which is mediated by the signaling molecules azelaic acid (AzA), glycerol-3-phosphate (G3P), and salicylic acid (SA). Negative regulator of plasmodesmata permeability triggered by SA during immune responses, through regulation of callose deposition. Delays the trafficking of Tobacco Mosaic Virus (TMV) movement protein (MP). Required for symplastic signal transport. KEYWORDS: 3D-structure;Cell junction;Cell membrane;Disulfide bond;Host-virus interaction;Membrane;Plant defense;Reference proteome;Repeat;Signal;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein Cell junction, plasmodesma Note=Located in cell wall junctions between leaf epidermal and mesophyl cells. Located to the central region of plasmodesmata. Co-localizes with the Grapevine fanleaf virus (GFLV) 2B-MP at the base of tubules within modified plasmodesmata. " Q8GUK1,"PROTEIN NAMES: Protein DGS1, mitochondrial (Protein DGD1 SUPPRESSOR 1) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Involved in galactoglycerolipid biosynthesis. Contributes to an intracellular signal that regulates an alternative DGD1-independent galactoglycerolipid biosynthesis pathway in chloroplasts. Being involved in mitochondrial lipid homeostasis, modulates mitochondrion biogenesis and physiology, as well as stress responses. KEYWORDS: Membrane;Mitochondrion;Mitochondrion outer membrane;Reference proteome;Transit peptide;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Mitochondrion outer membrane ; Multi-pass membrane protein " Q8GUK7,PROTEIN NAMES: E3 ubiquitin-protein ligase RMA3 (Protein RING membrane-anchor 3) (RING-type E3 ubiquitin transferase RMA3) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: E3 ubiquitin-protein ligase. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Endoplasmic reticulum;Membrane;Metal-binding;Reference proteome;Transferase;Transmembrane;Transmembrane helix;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Single-pass type IV membrane protein Q8GVD0,"PROTEIN NAMES: Oleuropein beta-glucosidase (Beta-glucosidase) (OeGLU) PROTEIN FAMILY: Glycosyl hydrolase 1 family ORGANISM: Olea europaea (Common olive) SIMILARITY: Belongs to the glycosyl hydrolase 1 family. FUNCTION: Major beta-glucosidase activating oleuropein into a potent protein cross-linking agent. No activity with rutin, luteolin or p-nitrophenyl-beta-glucopyranoside as substrates. KEYWORDS: Glycosidase;Hydrolase;Nucleus;Plant defense SUBCELLULAR LOCATION: Nucleus Note=Distinctive compartmentalization of the oleuropein/OeGLU dual partner is a requisite to ensure a correct defense system response and to prevent the autotoxicity. " Q8GW78,"PROTEIN NAMES: ATP-dependent Clp protease ATP-binding subunit CLPT2, chloroplastic PROTEIN FAMILY: ClpA/ClpB family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the ClpA/ClpB family. FUNCTION: Accessory protein regulating the assembly of the plastidial Clp protease system. CLPT1 first binds to the heptameric P-ring containing the CLP3-6 subunits followed by CLPT2, and only then does the P-ring combine with the R-ring composed of the clpP1 and CLPR1-4 subunits. Once the core complex is fully assembled, it then associates to the CLPC chaperone partner to form the functional protease. CLPT2 and CLPT1 are partially redundant. KEYWORDS: 3D-structure;Chloroplast;Plastid;Reference proteome;Repeat;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast " Q8GWD2,PROTEIN NAMES: Probable cyclic nucleotide-gated ion channel 12 (Cyclic nucleotide- and calmodulin-regulated ion channel 12) PROTEIN FAMILY: Cyclic nucleotide-gated cation channel family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the cyclic nucleotide-gated cation channel (TC 1.A.1.5) family. FUNCTION: Probable cyclic nucleotide-gated ion channel. KEYWORDS: Alternative splicing;Calmodulin-binding;cAMP;cAMP-binding;Cell membrane;cGMP;cGMP-binding;Ion channel;Ion transport;Ligand-gated ion channel;Membrane;Nucleotide-binding;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein MISCELLANEOUS: [Isoform 2]: May be due to a competing acceptor splice site. Q8GWW7,"PROTEIN NAMES: Agmatine deiminase (Agmatine iminohydrolase) (Protein EMBRYO DEFECTIVE 1873) PROTEIN FAMILY: Agmatine deiminase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the agmatine deiminase family. FUNCTION: Mediates the hydrolysis of agmatine into N-carbamoylputrescine in the arginine decarboxylase (ADC) pathway of putrescine biosynthesis, a basic polyamine. PATHWAY: Amine and polyamine biosynthesis; putrescine biosynthesis via agmatine pathway; N-carbamoylputrescine from agmatine: step 1/1. KEYWORDS: 3D-structure;Hydrolase;Polyamine biosynthesis;Reference proteome " Q8GWW8,"PROTEIN NAMES: Chaperone protein dnaJ GFA2, mitochondrial (Chaperone protein dnaJ A30) (AtDjA30) (Gametophytic factor 2) PROTEIN FAMILY: DnaJ family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the DnaJ family. FUNCTION: Chaperone that may play a role in mitochondrial protein folding. Involved in female gametophyte development. Required for cell death of the synergid cells during fertilization process, and fusion of the polar nuclei during megagametogenesis. KEYWORDS: Apoptosis;Chaperone;Metal-binding;Mitochondrion;Reference proteome;Repeat;Transit peptide;Zinc;Zinc-finger SUBCELLULAR LOCATION: Mitochondrion " Q8GXL3,"PROTEIN NAMES: Squamosa promoter-binding-like protein 8 ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Trans-acting factor that binds specifically to the consensus nucleotide sequence 5'-TNCGTACAA-3'. Binds specifically to the 5'-GTAC-3' core sequence. Involved in development and floral organogenesis. Required for ovule differentiation, pollen production, filament elongation, seed formation and siliques elongation. Also seems to play a role in the formation of trichomes on sepals. May positively modulate gibberellin (GA) signaling in flower. KEYWORDS: Alternative splicing;Cytoplasm;Developmental protein;Differentiation;DNA-binding;Flowering;Metal-binding;Nucleus;Reference proteome;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Cytoplasm Note=Mostly located in nucleus. " Q8GXR9,"PROTEIN NAMES: Alternative NAD(P)H-ubiquinone oxidoreductase C1, chloroplastic/mitochondrial (Alternative NADH dehydrogenase NDC1) (Demethylphylloquinone reductase NDC1) (NADH:ubiquinone reductase (non-electrogenic) NDC1) PROTEIN FAMILY: NADH dehydrogenase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the NADH dehydrogenase family. FUNCTION: Bifunctional oxidoreductase ables to act both on prenyl naphthoquinones and on prenyl benzoquinones. May serve a respiratory function. Involved in an electron flow toward the plastoglobule plastoquinone pool. Required for plastochromanol-8 accumulation and for phylloquinone (vitamin K1) production. Probably not directly involved in cyclic or chlororespiratory electron flows under standard growth conditions, but participates in the redox metabolism of plastoquinone-9 and the tocophrol recycling-intermediate alpha-tocopherol quinone. Catalyzes the penultimate step in the biosynthesis of vitamin K1. KEYWORDS: Alternative splicing;Chloroplast;FAD;Flavoprotein;Membrane;Mitochondrion;Mitochondrion inner membrane;NAD;NADP;Oxidoreductase;Plastid;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Mitochondrion Mitochondrion inner membrane ; Peripheral membrane protein ; Matrix side Plastid, chloroplast Plastid, chloroplast, plastoglobule " Q8GZB6,"PROTEIN NAMES: Histone-lysine N-methyltransferase, H3 lysine-9 specific SUVH4 (Histone H3-K9 methyltransferase 4) (H3-K9-HMTase 4) (Protein KRYPTONITE) (Protein SET DOMAIN GROUP 33) (Suppressor of variegation 3-9 homolog protein 4) (Su(var)3-9 homolog protein 4) PROTEIN FAMILY: Class V-like SAM-binding methyltransferase superfamily, Histone-lysine methyltransferase family, Suvar3-9 subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. Suvar3-9 subfamily. FUNCTION: Histone methyltransferase. Methylates 'Lys-9' of histone H3. H3 'Lys-9' methylation represents a specific tag for epigenetic transcriptional repression. The silencing mechanism via DNA CpNpG methylation requires the targeting of chromomethylase CMT3 to methylated histones, probably through an interaction with an HP1-like adapter. By its function, KYP is directly required for the maintenance of the DNA CpNpG and asymmetric methylation. Involved in the silencing of transposable elements. KEYWORDS: 3D-structure;Centromere;Chromatin regulator;Chromosome;Metal-binding;Methyltransferase;Nucleus;Reference proteome;S-adenosyl-L-methionine;Transferase;Zinc SUBCELLULAR LOCATION: Nucleus. Chromosome. Chromosome, centromere. Note=Associates with centromeric constitutive heterochromatin and at a lower level with regions of euchromatin. MISCELLANEOUS: Mutations in the KYP/SUVH4 gene decrease the level of histone H3-K9 dimethylated, trimethylated or dimethylated in association with H3-K14Ac by factors of 4,3 and 3, respectively. The level of monomethylated H3-K9 is unchanged. Such mutations lead to a drastic decrease of cytosine methylation at CpNpG sites, causing the reactivation of endogenous retrotransposons. The KRYPTONYTE methyltransferase name was given according to its involvement in SUPERMAN gene silencing." Q8H0W1,"PROTEIN NAMES: Chloroplast processing peptidase (Signal peptidase I-3) PROTEIN FAMILY: Peptidase S26 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the peptidase S26 family. FUNCTION: Involved in the maturation of the plastid protein translocation channel. Required for the biogenesis of plastid internal membranes. May also function as a thylakoidal processing peptidase. KEYWORDS: Chloroplast;Hydrolase;Membrane;Plastid;Protease;Reference proteome;Thylakoid;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast envelope Plastid, chloroplast thylakoid membrane Note=May be located at the envelope membranes in premature chloroplast and at the thylakoidal membrane in mature chloroplasts. " Q8H103,"PROTEIN NAMES: Glucose-6-phosphate isomerase 1, chloroplastic (GPI 1) (Phosphoglucose isomerase 1) (PGI 1) (Phosphohexose isomerase) (PHI) PROTEIN FAMILY: GPI family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the GPI family. FUNCTION: Promotes the synthesis of starch in leaves. PATHWAY: Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 2/4.; PATHWAY: Carbohydrate biosynthesis; gluconeogenesis. KEYWORDS: Alternative splicing;Chloroplast;Gluconeogenesis;Glycolysis;Isomerase;Phosphoprotein;Plastid;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast stroma " Q8H107,"PROTEIN NAMES: Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex 2, mitochondrial (2-oxoglutarate dehydrogenase complex component E2-2) (OGDC-E2-2) (Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex 2) (E2K-2) PROTEIN FAMILY: 2-oxoacid dehydrogenase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the 2-oxoacid dehydrogenase family. FUNCTION: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3) (By similarity). PATHWAY: Amino-acid degradation; L-lysine degradation via saccharopine pathway; glutaryl-CoA from L-lysine: step 6/6. KEYWORDS: Acyltransferase;Alternative splicing;Lipoyl;Mitochondrion;Reference proteome;Transferase;Transit peptide;Tricarboxylic acid cycle SUBCELLULAR LOCATION: Mitochondrion MISCELLANEOUS: [Isoform 3]: May be due to an intron retention." Q8H112,"PROTEIN NAMES: PGR5-like protein 1A, chloroplastic (Ferredoxin-plastoquinone reductase 1) PROTEIN FAMILY: PGR5 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the PGR5 family. FUNCTION: Ferredoxin-plastoquinone reductase involved in cyclic electron flow (CEF) around photosystem I. The homodimer is probably not involved in CEF. KEYWORDS: Alternative splicing;Chloroplast;Disulfide bond;Electron transport;Membrane;Plastid;Reference proteome;Thylakoid;Transit peptide;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane ; Multi-pass membrane protein Note=Predominantly located in the appressed regions of the thylakoids and less abundant in the stroma lamellae. MISCELLANEOUS: Thioredoxins prevent homodimerization." Q8H116,"PROTEIN NAMES: Mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS2 (AtMANIa) (Alpha-mannosidase IA) PROTEIN FAMILY: Glycosyl hydrolase 47 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the glycosyl hydrolase 47 family. FUNCTION: Class I alpha-mannosidase essential for early N-glycan processing. Progressively trims alpha-1,2-linked mannose residues. Produces Man(5)GlcNAc(2) from Man(8)GlcNAc(2), but only Man(6)GlcNAc(2) from Man(9)GlcNAc(2). Has difficulty acting on the terminal mannose of the b-branch. Involved in root development and cell wall biosynthesis. PATHWAY: Protein modification; protein glycosylation. KEYWORDS: Calcium;Coiled coil;Disulfide bond;Glycoprotein;Golgi apparatus;Hydrolase;Magnesium;Manganese;Membrane;Metal-binding;Reference proteome;Signal-anchor;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus membrane ; Single-pass type II membrane protein " Q8H151,PROTEIN NAMES: Malonate--CoA ligase (Acyl-activating enzyme 13) (Malonyl-CoA synthetase) PROTEIN FAMILY: ATP-dependent AMP-binding enzyme family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the ATP-dependent AMP-binding enzyme family. FUNCTION: Malonate--CoA ligase that catalyzes the formation of malonyl-CoA directly from malonate and CoA. May be required for the detoxification of malonate. KEYWORDS: Cytoplasm;Fatty acid metabolism;Ligase;Lipid metabolism;Nucleus;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Nucleus MISCELLANEOUS: Plants overexpressing AAE13 show increased sensitivity to exogenous malonate. Q8H1D4,PROTEIN NAMES: Double-stranded RNA-binding protein 4 (dsRNA-binding protein 4) (AtDRB4) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Double-stranded RNA-binding protein involved in RNA-mediated post-transcriptional gene silencing (PTGS). Functions in the trans-acting small interfering RNAs (ta-siRNAs) biogenesis by binding and assisting DICER-LIKE 4 (DCL4). Required for DCL4 activity. Required for the 21 nucleotide ta-siRNAs production of the TAS3 transcript in leaves but not in flowers. Plays an important role in silencing RNA of both DNA and RNA viruses. Involved with argonaute 7 (AGO7) and RDR6 in turnip crinkle virus (TCV) silencing. May not be directly involved in viral siRNA production. May stabilize the 21 nucleotide viral siRNAs and deliver them to the RISC complex. Targeted by the viral silencing suppressor (VSR) transactivator/viroplasmin (TAV) protein of the cauliflower mosaic virus (CaMV) that inactivates DRB4 function in RNA silencing. Probably not involved in the guide strand selection from RNA duplexes. Involved in leaf morphology through its function in ta-siRNA-mediated silencing. KEYWORDS: 3D-structure;Alternative splicing;Host-virus interaction;Nucleus;Plant defense;Reference proteome;Repeat;RNA-binding;RNA-mediated gene silencing SUBCELLULAR LOCATION: Nucleus Q8H1D6,"PROTEIN NAMES: Receptor-like cytosolic serine/threonine-protein kinase RBK1 (Protein ROP BINDING PROTEIN KINASES 1) PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. KEYWORDS: ATP-binding;Cytoplasm;Kinase;Membrane;Nucleotide-binding;Nucleus;Phosphoprotein;Reference proteome;Serine/threonine-protein kinase;Transferase SUBCELLULAR LOCATION: Cytoplasm Endomembrane system ; Peripheral membrane protein Nucleus " Q8H1F2,"PROTEIN NAMES: Regulator of G-protein signaling 1 (AtRGS1) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Glucose-regulated GTPase-accelerating protein (GAP) for the GTP-bound self-activating heterotrimeric G alpha protein GPA1. Cooperates with G beta-gamma dimers to maintain an unactivated but fully functional pool of GPA1. Phosphorylation-dependent endocytosis of RGS1 physically uncouples the two proteins, resulting in signal activation. Free AGB1 is essential, but not sufficient, for RGS1 endocytosis. Modulates cell proliferation, abscisic acid (ABA) and drought stress signal transduction by acting in a hexokinase-independent glucose-signaling pathway. Involved in the shapes of leaves, the development of floral buds, the elongation of stems, siliques, and hypocotyls, the time of flowering and the regulation of guard-cell K(+) and anion channels. Important for the kinetics of voltage activation of inward K(+) current but not for the current amplitude. KEYWORDS: Cell membrane;Endosome;Membrane;Phosphoprotein;Reference proteome;Signal transduction inhibitor;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Endosome membrane ; Multi-pass membrane protein Note=accumulates at the nascent cell plate during cytokinesis. " Q8H1G0,"PROTEIN NAMES: GATA transcription factor 28 (Protein TIFY 2A) (ZIM-like 2 protein) PROTEIN FAMILY: Type IV zinc-finger family, Class C subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the type IV zinc-finger family. Class C subfamily. FUNCTION: Transcriptional activator that specifically binds 5'-GATA-3' or 5'-GAT-3' motifs within gene promoters. KEYWORDS: Activator;DNA-binding;Metal-binding;Nucleus;Reference proteome;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus " Q8I0P7,"PROTEIN NAMES: Probable 3',5'-cyclic phosphodiesterase pde-3 PROTEIN FAMILY: Cyclic nucleotide phosphodiesterase family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the cyclic nucleotide phosphodiesterase family. KEYWORDS: Alternative splicing;cGMP;Hydrolase;Metal-binding;Reference proteome " Q8I295,"PROTEIN NAMES: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, apicoplast (HMBPP reductase) PROTEIN FAMILY: IspH family ORGANISM: Plasmodium falciparum (isolate 3D7) SIMILARITY: Belongs to the IspH family. FUNCTION: Catalyzes the conversion of 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate (HMBPP) into a mixture of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Acts in the terminal step of the DOXP/MEP pathway for isoprenoid precursor biosynthesis. PATHWAY: Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1.; PATHWAY: Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6. KEYWORDS: 3D-structure;4Fe-4S;Apicoplast;Iron;Iron-sulfur;Isoprene biosynthesis;Metal-binding;Oxidoreductase;Plastid;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Plastid, apicoplast " Q8I4B0,"PROTEIN NAMES: Potassium voltage-gated channel protein shk-1 PROTEIN FAMILY: Potassium channel family, A (Shaker) subfamily, Shaker sub-subfamily ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the potassium channel family. A (Shaker) (TC 1.A.1.2) subfamily. Shaker sub-subfamily. FUNCTION: Mediates the voltage-dependent potassium ion permeability of excitable membranes. Has an important role in repolarization and in regulating the pattern of action potential firing. Isoform a expresses currents in a more depolarized voltage range than isoform d. KEYWORDS: Alternative splicing;Ion channel;Ion transport;Membrane;Potassium;Potassium channel;Potassium transport;Reference proteome;Transmembrane;Transmembrane helix;Transport;Voltage-gated channel SUBCELLULAR LOCATION: Membrane ; Multi-pass membrane protein " Q8I4E2,"PROTEIN NAMES: Sorting nexin lst-4 (Lateral signaling target protein 4) PROTEIN FAMILY: Sorting nexin family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the sorting nexin family. FUNCTION: Involved in the signaling of vulval development by acting as a negative regulator of epidermal growth factor receptor (EGFR) signaling. Aids in phagosomal membrane tubule formation which is required for phagosomal fusion with endosomes and lysosomes. Also recruits rab-7 to phagosomes by an interaction with dyn-1. These are events leading to phagosome maturation which is a step in apoptotic cell corpse clearance. Binds phosphatidylinositol-3,4,5-trisphosphate. KEYWORDS: Alternative splicing;Cytoplasm;Cytoplasmic vesicle;Lipid-binding;Membrane;Reference proteome;SH3 domain SUBCELLULAR LOCATION: Cytoplasm Cytoplasmic vesicle, phagosome membrane Note=Recruited to phagosomal surface by phosphatidylinositol trisphosphate. BAR and PX domains are required for the phagosomal localization. " Q8I4V8,"PROTEIN NAMES: Peptidyl-prolyl cis-trans isomerase FKBP35 (PfFKBP35) PROTEIN FAMILY: FKBP-type PPIase family ORGANISM: Plasmodium falciparum (isolate 3D7) SIMILARITY: Belongs to the FKBP-type PPIase family. FUNCTION: Has peptidylprolyl isomerase (PPIase) and co-chaperone activities. Assists protein folding by catalyzing the peptidyl conversion of cis and trans rotamers of the prolyl amide bond of protein substrates. Inhibits calcineurin phosphatase activity in vitro. Plays an essential role in merozoite egress from host erythrocytes. KEYWORDS: 3D-structure;Chaperone;Cytoplasm;Isomerase;Nucleus;Reference proteome;Repeat;Rotamase;TPR repeat SUBCELLULAR LOCATION: Cytoplasm Nucleus Note=During the asexual blood stage, predominantly localizes to the cytoplasm of ring stage parasites and then translocates to the nucleus during the differentiation into trophozoites and schizonts. " Q8I6K0,"PROTEIN NAMES: Phenoloxidase-activating factor 3 (Prophenoloxidase-activating factor III) (Serine protease-like protein PPAF-3) [Cleaved into: Phenoloxidase-activating factor 3 light chain; Phenoloxidase-activating factor 3 heavy chain] PROTEIN FAMILY: Peptidase S1 family, CLIP subfamily ORGANISM: Holotrichia diomphalia (Korean black chafer) SIMILARITY: Belongs to the peptidase S1 family. CLIP subfamily. FUNCTION: Serine endopeptidase which, by cleaving prophenoloxidase activating factor PPAF2, is required for the activation of the prophenoloxidase cascade probably following the recognition of pathogen-derived products. KEYWORDS: Calcium;Direct protein sequencing;Disulfide bond;Glycoprotein;Hydrolase;Immunity;Innate immunity;Metal-binding;Protease;Secreted;Serine protease;Signal;Zymogen SUBCELLULAR LOCATION: Secreted Note=Secreted in the hemolymph. " Q8I7F8,"PROTEIN NAMES: Odorant response abnormal protein 4 PROTEIN FAMILY: ODR-4 family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the ODR-4 family. FUNCTION: Required, with oct-8, for the localization of a subset of 7 transmembrane domain odorant receptors, including odr-10, to the cilia of olfactory neurons AWA and AWC. Regulates chemotaxis responses to benzaldehyde, diacetyl and 2,4,5-trimethylthiazole in AWA and AWC neurons. Plays a role in nociceptive neurons to mediate avoidance of high oxygen. KEYWORDS: Alternative splicing;Cell projection;Chemotaxis;Cytoplasmic vesicle;Endoplasmic reticulum;Golgi apparatus;Membrane;Olfaction;Reference proteome;Sensory transduction;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Cytoplasmic vesicle, secretory vesicle membrane; Multi-pass membrane protein. Cell projection, dendrite. Cell projection, axon. Note=Punctate expression in dendrite and axons. " Q8I7M8,"PROTEIN NAMES: Cyclin-dependent kinase 17 (Cell division protein kinase 17) (PCTAIRE-motif protein kinase) PROTEIN FAMILY: Protein kinase superfamily, CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. FUNCTION: Serine/threonine-protein kinase, which, in association with cyy-1, regulates the trafficking of synaptic vesicles in the DA9 motor neuron and probably also in the DD motor neurons and in RIA interneurons.; FUNCTION: [Isoform c]: Sufficient for synaptic vesicle trafficking in the DA9 motor neuron. KEYWORDS: Alternative splicing;ATP-binding;Cell projection;Cytoplasm;Kinase;Magnesium;Metal-binding;Nucleotide-binding;Reference proteome;Serine/threonine-protein kinase;Transferase SUBCELLULAR LOCATION: Cytoplasm Cell projection, dendrite Cell projection, axon Note=Evenly distributed in the dendrite, the axon and the cell body of the DA motor neuron. " Q8I8V0,"PROTEIN NAMES: Transcriptional adapter 2B (dADA2b) ORGANISM: Drosophila melanogaster (Fruit fly) FUNCTION: Required for the function of some acidic activation domains, which activate transcription from a distant site. Binds double-stranded DNA. Binds dinucleosomes, probably at the linker region between neighboring nucleosomes. Plays a role in chromatin remodeling. KEYWORDS: Alternative splicing;DNA-binding;Metal-binding;Nucleus;Reference proteome;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus " Q8IE47,"PROTEIN NAMES: NAD-dependent protein deacylase Sir2A (PfSir2) (PfSir2A) (Regulatory protein SIR2 homolog A) PROTEIN FAMILY: Sirtuin family, Class III subfamily ORGANISM: Plasmodium falciparum (isolate 3D7) SIMILARITY: Belongs to the sirtuin family. Class III subfamily. FUNCTION: NAD-dependent protein deacylase. Catalyzes the NAD-dependent hydrolysis of medium and long chain fatty acyl groups from lysine residues. Has weak NAD-dependent protein deacetylase activity. Regulates the endonuclease activity of ALBA3 by deacetylation of 'Lys-23'. Regulates the expression of the surface antigen-coding var genes central to the malaria pathogenesis. Cooperates with Sir2B to mediate silencing and mutual exclusive expression of only 1 of the 60 subtelomeric var genes at a time, coding for functionally different but epitopically variant versions of the erythrocyte membrane protein 1 (PfEMP1) molecule, to evade the detection by host immune surveillance. Involved in recruiting ORC1 to the telomers and subtelomeric repeat regions (TAREs) and promoters of var genes. Can ADP-ribosylate both histones and itself. May also have a role in telomeric end protection. KEYWORDS: 3D-structure;Chromosome;Metal-binding;NAD;Nucleus;Reference proteome;Telomere;Transferase;Zinc SUBCELLULAR LOCATION: Nucleus Nucleus, nucleolus Chromosome, telomere Note=At the late ring stage/early trophozoites, co-localizes with ORC1 to telomeres and subtelomeric regions (TAREs) in the nuclear periphery. Spreads out from the telomere over a distance of at least 20-40 kb and is an important component of the heterochromatin complex around Rep20, a region that lies adjacent to the regulatory 5'-UTR element of telomeric var genes. May relocate to the cytoplasm during the multiple rounds of DNA synthesis and nuclear mitosis in trophozoite stage. MISCELLANEOUS: The reported ADP-ribosyltransferase activity of sirtuins is likely to be some inefficient side reaction of the deacetylase activity and may not be physiologically relevant." Q8IFM8,"PROTEIN NAMES: Cysteine-rich protective antigen (Inactive sialidase CyRPA) ORGANISM: Plasmodium falciparum (isolate 3D7) FUNCTION: Essential for the invasion of host erythrocytes by blood stage merozoites. Required for the assembly of the PfRH5 adhesion complex (or invasion complex) composed of CyRPA, RH5 and RIPR at the interface between the merozoite and the host erythrocyte membranes. This facilitates the binding of RH5 to host receptor BSG/basigin, which leads to the establishment of a tight junction between the merozoite and host erythrocyte membranes and allows Ca(2+) release into the erythrocyte. KEYWORDS: 3D-structure;Cell membrane;Cytoplasmic vesicle;Disulfide bond;Glycoprotein;Host cell membrane;Host membrane;Membrane;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted Cytoplasmic vesicle, secretory vesicle, microneme lumen Cell membrane ; Peripheral membrane protein ; Extracellular side Host cell membrane Note=In late schizonts, colocalizes with RIPR in the microneme lumen. During merozoite invasion of host erythrocytes, secreted at the merozoite apical surface where it colocalizes with RIPR and RH5 at the interface between the merozoite and the erythrocyte. " Q8IGJ0,PROTEIN NAMES: Protein EFR3 homolog cmp44E (Conserved membrane protein at 44E) (Protein stambha A) PROTEIN FAMILY: EFR3 family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the EFR3 family. FUNCTION: An essential gene required for embryogenesis; required for cell viability. KEYWORDS: Alternative splicing;Membrane;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Single-pass membrane protein Q8IIJ6,PROTEIN NAMES: Ubiquitin carboxyl-terminal hydrolase UCH54 (PfUCH37) (PfUCH54) PROTEIN FAMILY: Peptidase C12 family ORGANISM: Plasmodium falciparum (isolate 3D7) SIMILARITY: Belongs to the peptidase C12 family. FUNCTION: Thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. KEYWORDS: Hydrolase;Protease;Reference proteome;Thiol protease;Ubl conjugation pathway Q8IIJ9,"PROTEIN NAMES: Dipeptidyl aminopeptidase 1 (Cathepsin C homolog) [Cleaved into: Dipeptidyl aminopeptidase 1 exclusion domain chain; Dipeptidyl aminopeptidase 1 heavy chain; Dipeptidyl aminopeptidase 1 light chain] PROTEIN FAMILY: Peptidase C1 family ORGANISM: Plasmodium falciparum (isolate 3D7) SIMILARITY: Belongs to the peptidase C1 family. FUNCTION: Thiol protease that cleaves dipeptides from the N-terminus of protein substrates. Active against a broad range of dipeptide substrates composed of both polar and hydrophobic amino acids. Proline cannot occupy the P1 position and arginine or lysine cannot occupy the P2 position of the substrate. Involved in host hemoglobin degradation by generating dipeptides from hemoglobin-derived oligopeptides. KEYWORDS: Chloride;Direct protein sequencing;Disulfide bond;Glycoprotein;Hydrolase;Protease;Reference proteome;Signal;Sulfation;Thiol protease;Vacuole;Zymogen SUBCELLULAR LOCATION: Vacuole lumen Parasitophorous vacuole lumen Note=In schizonts, the immature form localizes to the parasitophorous vacuole. In trophozoites, the mature form localizes to the digestive (or food) vacuole, an acidic vacuole where host hemoglobin is digested. " Q8IJA9,"PROTEIN NAMES: Adenosine deaminase (S-methyl-5'-thioadenosine deaminase) PROTEIN FAMILY: Metallo-dependent hydrolases superfamily, Adenosine and AMP deaminases family ORGANISM: Plasmodium falciparum (isolate 3D7) SIMILARITY: Belongs to the metallo-dependent hydrolases superfamily. Adenosine and AMP deaminases family. FUNCTION: Catalyzes the hydrolytic deamination of adenosine to produce inosine. Unlike mammalian adenosine deaminases, also catalyzes the deamination of 5'-methylthioadenosine (MTA), a by-product of polyamine biosynthesis, to produce 5'-methylthioinosine (MTI). Plays an essential role in the purine salvage pathway which allows the parasite to use host cell purines for the synthesis of nucleic acids (Probable). PATHWAY: Purine metabolism; purine nucleoside salvage. KEYWORDS: 3D-structure;Hydrolase;Metal-binding;Purine salvage;Reference proteome;Zinc " Q8IJP2,"PROTEIN NAMES: Methionine aminopeptidase 1b (PfMetAP1b) PROTEIN FAMILY: Peptidase M24A family, Methionine aminopeptidase type 1 subfamily ORGANISM: Plasmodium falciparum (isolate 3D7) SIMILARITY: Belongs to the peptidase M24A family. Methionine aminopeptidase type 1 subfamily. FUNCTION: Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val) (By similarity). May play an important role in parasite growth during the blood asexual stage. KEYWORDS: 3D-structure;Aminopeptidase;Cytoplasm;Hydrolase;Metal-binding;Protease;Reference proteome;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cytoplasm " Q8IKM8,PROTEIN NAMES: Ubiquitin carboxyl-terminal hydrolase UCHL3 (PfUCHL3) PROTEIN FAMILY: Peptidase C12 family ORGANISM: Plasmodium falciparum (isolate 3D7) SIMILARITY: Belongs to the peptidase C12 family. FUNCTION: Thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. Essential for parasite blood stage survival. KEYWORDS: 3D-structure;Hydrolase;Protease;Reference proteome;Thiol protease;Ubl conjugation pathway Q8IMX7,"PROTEIN NAMES: Mitochondrial Rho GTPase (Miro) (dMiro) PROTEIN FAMILY: Mitochondrial Rho GTPase family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the mitochondrial Rho GTPase family. FUNCTION: Mitochondrial GTPase involved in mitochondrial trafficking. Forms an essential protein complex with Milt that links Khc to mitochondria for light chain-independent, anterograde transport of mitochondria. Required for axonal transport to synapses within nerve terminals. Required presynaptically but not postsynaptically at neuromuscular junctions (NMJs). Also involved in the regulation of mitochondrial dynamics by promoting drp1-mediated mitochondrial fission during high calcium conditions, and negatively regulating fission during normal conditions. KEYWORDS: 3D-structure;Alternative splicing;Calcium;GTP-binding;Hydrolase;Membrane;Metal-binding;Mitochondrion;Mitochondrion outer membrane;Nucleotide-binding;Reference proteome;Repeat;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Mitochondrion outer membrane ; Single-pass type IV membrane protein " Q8IMZ9,"PROTEIN NAMES: Wolframin ORGANISM: Drosophila melanogaster (Fruit fly) FUNCTION: Participates in the regulation of cellular Ca(2+) homeostasis, at least partly, by modulating the filling state of the endoplasmic reticulum Ca(2+) store (By similarity). In neurons and glial cells, has a role in maintaining neuronal function and integrity during aging. KEYWORDS: Alternative splicing;Endoplasmic reticulum;Glycoprotein;Membrane;Mitochondrion;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Multi-pass membrane protein Endoplasmic reticulum Mitochondrion " Q8INK9,"PROTEIN NAMES: Methionine-R-sulfoxide reductase B1 (Selenoprotein R) PROTEIN FAMILY: MsrB Met sulfoxide reductase family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the MsrB Met sulfoxide reductase family. FUNCTION: Methionine-sulfoxide reductase that specifically reduces methionine (R)-sulfoxide back to methionine. While in many cases methionine oxidation is the result of random oxidation following oxidative stress, methionine oxidation is also a post-translational modification that takes place on specific residues. Acts as a regulator of actin assembly by reducing methionine (R)-sulfoxide mediated by Mical on actin thereby promoting filament repolymerization. KEYWORDS: Alternative splicing;Cytoplasm;Cytoskeleton;Disulfide bond;Metal-binding;Nucleus;Oxidoreductase;Reference proteome;Zinc SUBCELLULAR LOCATION: Cytoplasm Nucleus Cytoplasm, cytoskeleton " Q8IPH9,"PROTEIN NAMES: Slowpoke-binding protein ORGANISM: Drosophila melanogaster (Fruit fly) FUNCTION: Regulator of calcium-activated channel Slo. Increases or decreases the voltage sensitivity of Slo, depending on the absence or presence of 14-3-3-zeta in the complex, respectively. KEYWORDS: Alternative splicing;Cytoplasm;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Note=Enriched in synaptic boutons. " Q8IQG1,"PROTEIN NAMES: MOB kinase activator-like 2 (Mob as tumor suppressor protein 2) (Dmob2) (Mps one binder kinase activator-like 2) PROTEIN FAMILY: MOB1/phocein family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the MOB1/phocein family. FUNCTION: Required for the normal morphogenesis of a variety of polarized outgrowths including epidermal hairs, bristles, arista laterals, and dendrites. KEYWORDS: Alternative splicing;Cytoplasm;Metal-binding;Nucleus;Reference proteome;RNA editing;Zinc SUBCELLULAR LOCATION: Cytoplasm Nucleus Note=Trc colocalizes with Mob1 to the cell periphery in wing cells and wing hairs. " Q8IRH5,"PROTEIN NAMES: Zinc finger protein indra PROTEIN FAMILY: Krueppel C2H2-type zinc-finger protein family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein family. FUNCTION: Required for rDNA copy number maintenance and non-random sister chromatid segregation (NRSS) following unequal sister chromatid exchange. Binds ribosomal DNA (rDNA) preferentially binding to intergenic spacers (IGS) regions on both X and Y chromosomes. Essential for NRSS, a mechanism which contributes to the recovery and maintenance of inherently unstable rDNA copy numbers so that the integrity of the germline genome is upheld over generations and germline immortality is sustained. May be involved in transcriptional regulation (Probable). KEYWORDS: Alternative splicing;DNA-binding;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Repeat;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Nucleus, nucleolus Note=Localizes to the nucleolus (the site of rDNA transcription) during interphase and rDNA loci during metaphase. MISCELLANEOUS: Named after the Hindu god Indra who lost immortality due to a curse from Durvasa, likely referring to mutants displaying a loss of germline immortality." Q8IU54,"PROTEIN NAMES: Interferon lambda-1 (IFN-lambda-1) (Cytokine Zcyto21) (Interleukin-29) (IL-29) PROTEIN FAMILY: Lambda interferon family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the lambda interferon family. FUNCTION: Cytokine with antiviral, antitumour and immunomodulatory activities. Plays a critical role in the antiviral host defense, predominantly in the epithelial tissues. Acts as a ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1, and receptor engagement leads to the activation of the JAK/STAT signaling pathway resulting in the expression of IFN-stimulated genes (ISG), which mediate the antiviral state. Has a restricted receptor distribution and therefore restricted targets: is primarily active in epithelial cells and this cell type-selective action is because of the epithelial cell-specific expression of its receptor IFNLR1. Exerts an immunomodulatory effect by up-regulating MHC class I antigen expression. KEYWORDS: 3D-structure;Antiviral defense;Cytokine;Disulfide bond;Glycoprotein;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted. " Q8IUD6,"PROTEIN NAMES: E3 ubiquitin-protein ligase RNF135 (RIG-I E3 ubiquitin ligase) (REUL) (RING finger protein 135) (RING finger protein leading to RIG-I activation) (Riplet) (RING-type E3 ubiquitin transferase RNF135) ORGANISM: Homo sapiens (Human) FUNCTION: E2-dependent E3 ubiquitin-protein ligase that functions as a RIGI coreceptor in the sensing of viral RNAs in cell cytoplasm and the activation of the antiviral innate immune response. Together with the UBE2D3, UBE2N and UB2V1 E2 ligases, catalyzes the 'Lys-63'-linked polyubiquitination of RIGI oligomerized on viral RNAs, an essential step in the activation of the RIG-I signaling pathway. Through a ubiquitin-independent parallel mechanism, which consists in bridging RIGI filaments forming on longer viral RNAs, further activates the RIG-I signaling pathway. This second mechanism that synergizes with the ubiquitin-dependent one would thereby allow an RNA length-dependent regulation of the RIG-I signaling pathway (Probable). Associated with the E2 ligase UBE2N, also constitutively synthesizes unanchored 'Lys-63'-linked polyubiquitin chains that may also activate the RIG-I signaling pathway. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: 3D-structure;Alternative splicing;Coiled coil;Cytoplasm;Disease variant;Immunity;Innate immunity;Metal-binding;Reference proteome;Transferase;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cytoplasm Cytoplasm, Stress granule " Q8IUN9,"PROTEIN NAMES: C-type lectin domain family 10 member A (C-type lectin superfamily member 14) (Macrophage lectin 2) (CD antigen CD301) ORGANISM: Homo sapiens (Human) FUNCTION: Probable role in regulating adaptive and innate immune responses. Binds in a calcium-dependent manner to terminal galactose and N-acetylgalactosamine units, linked to serine or threonine. These sugar moieties are known as Tn-Ag and are expressed in a variety of carcinoma cells. KEYWORDS: 3D-structure;Adaptive immunity;Alternative splicing;Coiled coil;Disulfide bond;Endocytosis;Glycoprotein;Immunity;Innate immunity;Lectin;Membrane;Reference proteome;Signal-anchor;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Single-pass type II membrane protein " Q8IV53,"PROTEIN NAMES: DENN domain-containing protein 1C (Connecdenn 3) (Protein FAM31C) ORGANISM: Homo sapiens (Human) FUNCTION: Guanine nucleotide exchange factor (GEF) which may activate RAB8A, RAB13 and RAB35. Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form. KEYWORDS: Alternative splicing;Cytoplasm;Cytoplasmic vesicle;Guanine-nucleotide releasing factor;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cytoplasm, cytosol Cytoplasmic vesicle, clathrin-coated vesicle " Q8IV76,"PROTEIN NAMES: Circadian clock protein PASD1 (Cancer/testis antigen 63) (CT63) (OX-TES-1) (PAS domain-containing protein 1) ORGANISM: Homo sapiens (Human) FUNCTION: Functions as a suppressor of the biological clock that drives the daily circadian rhythms of cells throughout the body. Acts as a nuclear repressor of the CLOCK-BMAL1 heterodimer-mediated transcriptional activation of the core clock components. Inhibits circadian clock function in cancer cells, when overexpressed. KEYWORDS: Alternative splicing;Biological rhythms;Coiled coil;Nucleus;Reference proteome SUBCELLULAR LOCATION: [Isoform 1]: Nucleus Note=Associates preferentially at the periphery of the nucleus with heterochromatin.; SUBCELLULAR LOCATION: [Isoform 2]: Nucleus Note=Associates preferentially at the periphery of the nucleus with heterochromatin. MISCELLANEOUS: [Isoform 2]: Due to intron retention." Q8IVH4,"PROTEIN NAMES: Methylmalonic aciduria type A protein, mitochondrial PROTEIN FAMILY: SIMIBI class G3E GTPase family, ArgK/MeaB subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the SIMIBI class G3E GTPase family. ArgK/MeaB subfamily. FUNCTION: GTPase, binds and hydrolyzes GTP. Involved in intracellular vitamin B12 metabolism, mediates the transport of cobalamin (Cbl) into mitochondria for the final steps of adenosylcobalamin (AdoCbl) synthesis. Functions as a G-protein chaperone that assists AdoCbl cofactor delivery from MMAB to the methylmalonyl-CoA mutase (MMUT). Plays a dual role as both a protectase and a reactivase for MMUT. Protects MMUT from progressive inactivation by oxidation by decreasing the rate of the formation of the oxidized inactive cofactor hydroxocobalamin (OH2Cbl). Additionally acts a reactivase by promoting the replacement of OH2Cbl by the active cofactor AdoCbl, restoring the activity of MMUT in the presence and hydrolysis of GTP. KEYWORDS: 3D-structure;Chaperone;Cytoplasm;Disease variant;GTP-binding;Hydrolase;Mitochondrion;Nucleotide-binding;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Mitochondrion Cytoplasm " Q8IVM0,PROTEIN NAMES: Coiled-coil domain-containing protein 50 (Protein Ymer) ORGANISM: Homo sapiens (Human) FUNCTION: Involved in EGFR signaling. KEYWORDS: 3D-structure;Acetylation;Alternative splicing;Coiled coil;Cytoplasm;Deafness;Non-syndromic deafness;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cytoplasm. Note=Associated with microtubules of the cytoskeleton and mitotic apparatus. MISCELLANEOUS: Found in a critical region of hereditary spastic paraplegia (HSP) SPG14 locus. No causative CCDC50 mutations were found in HSP families.; MISCELLANEOUS: [Isoform 1]: Major isoform. Q8IVT5,"PROTEIN NAMES: Kinase suppressor of Ras 1 PROTEIN FAMILY: Protein kinase superfamily, TKL Ser/Thr protein kinase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. FUNCTION: Part of a multiprotein signaling complex which promotes phosphorylation of Raf family members and activation of downstream MAP kinases (By similarity). Independently of its kinase activity, acts as MAP2K1/MEK1 and MAP2K2/MEK2-dependent allosteric activator of BRAF; upon binding to MAP2K1/MEK1 or MAP2K2/MEK2, dimerizes with BRAF and promotes BRAF-mediated phosphorylation of MAP2K1/MEK1 and/or MAP2K2/MEK2. Promotes activation of MAPK1 and/or MAPK3, both in response to EGF and to cAMP (By similarity). Its kinase activity is unsure (By similarity). Some protein kinase activity has been detected in vitro, however the physiological relevance of this activity is unknown (By similarity). KEYWORDS: 3D-structure;Alternative splicing;ATP-binding;Cell membrane;Cell projection;Cytoplasm;Endoplasmic reticulum;Kinase;Membrane;Metal-binding;Nucleotide-binding;Phosphoprotein;Reference proteome;Serine/threonine-protein kinase;Transferase;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cytoplasm Membrane ; Peripheral membrane protein Cell membrane ; Peripheral membrane protein Cell projection, ruffle membrane Endoplasmic reticulum membrane Note=In unstimulated cells, where the phosphorylated form is bound to a 14-3-3 protein, sequestration in the cytoplasm occurs. Following growth factor treatment, the protein is free for membrane translocation, and it moves from the cytoplasm to the cell periphery. " Q8IW40,"PROTEIN NAMES: Coiled-coil domain-containing protein 103 PROTEIN FAMILY: CCDC103/PR46b family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the CCDC103/PR46b family. FUNCTION: Dynein-attachment factor required for cilia motility. KEYWORDS: Alternative splicing;Cell projection;Ciliopathy;Cilium;Cilium biogenesis/degradation;Coiled coil;Cytoplasm;Disease variant;Flagellum;Kartagener syndrome;Primary ciliary dyskinesia;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Cell projection, cilium, flagellum " Q8IWL3,"PROTEIN NAMES: Iron-sulfur cluster co-chaperone protein HscB (DnaJ homolog subfamily C member 20) [Cleaved into: Iron-sulfur cluster co-chaperone protein HscB, cytoplasmic (C-HSC20); Iron-sulfur cluster co-chaperone protein HscB, mitochondrial] PROTEIN FAMILY: HscB family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the HscB family. FUNCTION: [Iron-sulfur cluster co-chaperone protein HscB, mitochondrial]: Acts as a co-chaperone in iron-sulfur cluster assembly in mitochondria. Required for incorporation of iron-sulfur clusters into SDHB, the iron-sulfur protein subunit of succinate dehydrogenase that is involved in complex II of the mitochondrial electron transport chain. Recruited to SDHB by interaction with SDHAF1 which first binds SDHB and then recruits the iron-sulfur transfer complex formed by HSC20, HSPA9 and ISCU through direct binding to HSC20. Plays an essential role in hematopoiesis (By similarity).; FUNCTION: [Iron-sulfur cluster co-chaperone protein HscB, cytoplasmic]: Acts as a co-chaperone in iron-sulfur cluster assembly in the cytoplasm. Also mediates complex formation between components of the cytosolic iron-sulfur biogenesis pathway and the CIA targeting complex composed of CIAO1, DIPK1B/FAM69B and MMS19 by binding directly to the scaffold protein ISCU and to CIAO1. This facilitates iron-sulfur cluster insertion into a number of cytoplasmic and nuclear proteins including POLD1, ELP3, DPYD and PPAT. PATHWAY: Cofactor biosynthesis; iron-sulfur cluster biosynthesis. KEYWORDS: 3D-structure;Chaperone;Cytoplasm;Metal-binding;Mitochondrion;Reference proteome SUBCELLULAR LOCATION: [Iron-sulfur cluster co-chaperone protein HscB, cytoplasmic]: Cytoplasm.; SUBCELLULAR LOCATION: [Iron-sulfur cluster co-chaperone protein HscB, mitochondrial]: Mitochondrion " Q8IWZ6,"PROTEIN NAMES: Bardet-Biedl syndrome 7 protein (BBS2-like protein 1) ORGANISM: Homo sapiens (Human) FUNCTION: The BBSome complex is thought to function as a coat complex required for sorting of specific membrane proteins to the primary cilia. The BBSome complex is required for ciliogenesis but is dispensable for centriolar satellite function. This ciliogenic function is mediated in part by the Rab8 GDP/GTP exchange factor, which localizes to the basal body and contacts the BBSome. Rab8(GTP) enters the primary cilium and promotes extension of the ciliary membrane. Firstly the BBSome associates with the ciliary membrane and binds to RAB3IP/Rabin8, the guanosyl exchange factor (GEF) for Rab8 and then the Rab8-GTP localizes to the cilium and promotes docking and fusion of carrier vesicles to the base of the ciliary membrane. The BBSome complex, together with the LTZL1, controls SMO ciliary trafficking and contributes to the sonic hedgehog (SHH) pathway regulation. Required for proper BBSome complex assembly and its ciliary localization. KEYWORDS: Acetylation;Alternative splicing;Bardet-Biedl syndrome;Cell membrane;Cell projection;Ciliopathy;Cilium;Cilium biogenesis/degradation;Cytoplasm;Cytoskeleton;Disease variant;Intellectual disability;Membrane;Obesity;Protein transport;Reference proteome;Sensory transduction;Transport;Vision SUBCELLULAR LOCATION: Cell projection, cilium membrane Cytoplasm Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriolar satellite Cytoplasm, cytoskeleton, cilium basal body " Q8IWZ8,PROTEIN NAMES: SURP and G-patch domain-containing protein 1 (RNA-binding protein RBP) (Splicing factor 4) ORGANISM: Homo sapiens (Human) FUNCTION: Plays a role in pre-mRNA splicing. KEYWORDS: 3D-structure;Alternative splicing;mRNA processing;mRNA splicing;Nucleus;Phosphoprotein;Reference proteome;Repeat;Spliceosome SUBCELLULAR LOCATION: Nucleus Q8IXF0,PROTEIN NAMES: Neuronal PAS domain-containing protein 3 (Neuronal PAS3) (Basic-helix-loop-helix-PAS protein MOP6) (Class E basic helix-loop-helix protein 12) (bHLHe12) (Member of PAS protein 6) (PAS domain-containing protein 6) ORGANISM: Homo sapiens (Human) FUNCTION: May play a broad role in neurogenesis. May control regulatory pathways relevant to schizophrenia and to psychotic illness (By similarity). KEYWORDS: Alternative splicing;Chromosomal rearrangement;DNA-binding;Nucleus;Reference proteome;Repeat;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus Q8IXH8,"PROTEIN NAMES: Cadherin-like protein 26 (Cadherin-like protein VR20) ORGANISM: Homo sapiens (Human) FUNCTION: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Ligand for integrins alpha-E/beta-7, ITGAE:ITGAB7, alpha-4/beta-7, ITGA4:ITGAB7 and alpha-4/beta-1, ITGA4:ITGAB1 through which modulates CD4(+) T cells activation. KEYWORDS: Alternative splicing;Calcium;Cell adhesion;Cell membrane;Glycoprotein;Membrane;Metal-binding;Reference proteome;Repeat;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein " Q8IXL6,"PROTEIN NAMES: Extracellular serine/threonine protein kinase FAM20C (Dentin matrix protein 4) (DMP-4) (Golgi casein kinase) (Golgi-enriched fraction casein kinase) (GEF-CK) PROTEIN FAMILY: FAM20 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the FAM20 family. FUNCTION: Golgi serine/threonine protein kinase that phosphorylates secretory pathway proteins within Ser-x-Glu/pSer motifs and plays a key role in biomineralization of bones and teeth. Constitutes the main protein kinase for extracellular proteins, generating the majority of the extracellular phosphoproteome. Mainly phosphorylates proteins within the Ser-x-Glu/pSer motif, but also displays a broader substrate specificity. Phosphorylates ERO1A, enhancing its activity which is required to maintain endoplasmic reticulum redox homeostasis and for oxidative protein folding. During endoplasmic reticulum stress, phosphorylates P4HB/PDIA1 which induces a functional switch, causing P4HB to change from an oxidoreductase to a molecular chaperone. This is critical to maintain ER proteostasis and reduce cell death under ER stress. Phosphorylation of P4HB also promotes its interaction with ERN1, leading to reduced activity of ERN1, a key sensor for the endoplasmic reticulum unfolded protein response. Required for osteoblast differentiation and mineralization. Phosphorylates casein as well as a number of proteins involved in biomineralization such as AMELX, AMTN, ENAM and SPP1/OPN. In addition to its role in biomineralization, also plays a role in lipid homeostasis, wound healing and cell migration and adhesion. KEYWORDS: 3D-structure;Alternative splicing;ATP-binding;Biomineralization;Calcium;Direct protein sequencing;Disease variant;Disulfide bond;Endoplasmic reticulum;Glycoprotein;Golgi apparatus;Kinase;Manganese;Membrane;Metal-binding;Nucleotide-binding;Phosphoprotein;Reference proteome;Secreted;Serine/threonine-protein kinase;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus membrane ; Single-pass type II membrane protein Secreted Endoplasmic reticulum Note=Resides in the Golgi apparatus membrane and is secreted following propeptide cleavage. Retained in the endoplasmic reticulum (ER) in response to ER stress where it phosphorylates P4HB. " Q8IYB1,PROTEIN NAMES: Nucleotidyltransferase MB21D2 (Mab-21 domain-containing protein 2) (hMB21D2) PROTEIN FAMILY: Mab-21 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the mab-21 family. FUNCTION: Probable nucleotidyltransferase that catalyzes the formation of cyclic dinucleotide second messenger in response to some unknown stimulus. KEYWORDS: 3D-structure;Direct protein sequencing;Nucleotidyltransferase;Phosphoprotein;Reference proteome;Transferase Q8IYB4,"PROTEIN NAMES: PEX5-related protein (PEX2-related protein) (PEX5-like protein) (Peroxin-5-related protein) (Peroxisome biogenesis factor 5-like) (Tetratricopeptide repeat-containing Rab8b-interacting protein) (Pex5Rp) (TRIP8b) PROTEIN FAMILY: Peroxisomal targeting signal receptor family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the peroxisomal targeting signal receptor family. FUNCTION: Accessory subunit of hyperpolarization-activated cyclic nucleotide-gated (HCN) channels, regulating their cell-surface expression and cyclic nucleotide dependence. KEYWORDS: Alternative splicing;Cytoplasm;Membrane;Phosphoprotein;Reference proteome;Repeat;TPR repeat SUBCELLULAR LOCATION: Cytoplasm Membrane ; Peripheral membrane protein Note=Some fraction is membrane associated via its interaction with RAB8B. " Q8IYS5,PROTEIN NAMES: Osteoclast-associated immunoglobulin-like receptor (Osteoclast-associated receptor) (hOSCAR) (Polymeric immunoglobulin receptor 3) (PIgR-3) (PIgR3) (Poly-Ig receptor 3) PROTEIN FAMILY: Leukocyte receptor complex/polymeric immunoglobulin receptor (PIR/LRC) family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the leukocyte receptor complex/polymeric immunoglobulin receptor (PIR/LRC) family. FUNCTION: Regulator of osteoclastogenesis which plays an important bone-specific function in osteoclast differentiation. KEYWORDS: 3D-structure;Alternative splicing;Cell membrane;Disulfide bond;Glycoprotein;Immunoglobulin domain;Membrane;Receptor;Reference proteome;Repeat;Secreted;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: [Isoform 1]: Secreted.; SUBCELLULAR LOCATION: [Isoform 2]: Cell membrane ; Single-pass type I membrane protein Q8IYU8,"PROTEIN NAMES: Calcium uptake protein 2, mitochondrial (EF-hand domain-containing family member A1) PROTEIN FAMILY: MICU1 family, MICU2 subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the MICU1 family. MICU2 subfamily. FUNCTION: Key regulator of mitochondrial calcium uniporter (MCU) required to limit calcium uptake by MCU when cytoplasmic calcium is low. MICU1 and MICU2 form a disulfide-linked heterodimer that stimulate and inhibit MCU activity, depending on the concentration of calcium. MICU2 acts as a gatekeeper of MCU that senses calcium level via its EF-hand domains: prevents channel opening at resting calcium, avoiding energy dissipation and cell-death triggering. KEYWORDS: 3D-structure;Disulfide bond;Mitochondrion;Phosphoprotein;Reference proteome;Repeat;Transit peptide SUBCELLULAR LOCATION: Mitochondrion intermembrane space " Q8IZ41,"PROTEIN NAMES: Ras and EF-hand domain-containing protein (Ras-related protein Rab-45) PROTEIN FAMILY: Small GTPase superfamily, Rab family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the small GTPase superfamily. Rab family. FUNCTION: Binds predominantly GDP, and also GTP. Acts as a dynein adapter protein that activates dynein-mediated transport and dynein-dynactin motility on microtubules. KEYWORDS: 3D-structure;Alternative splicing;Calcium;Coiled coil;Cytoplasm;GTP-binding;Metal-binding;Nucleotide-binding;Phosphoprotein;Reference proteome;Repeat SUBCELLULAR LOCATION: Cytoplasm, perinuclear region " Q8IZC7,PROTEIN NAMES: Zinc finger protein 101 (Zinc finger protein HZF12) PROTEIN FAMILY: Krueppel C2H2-type zinc-finger protein family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein family. FUNCTION: May be involved in transcriptional regulation. KEYWORDS: Alternative splicing;DNA-binding;Metal-binding;Nucleus;Reference proteome;Repeat;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Q8IZD6,"PROTEIN NAMES: Solute carrier family 22 member 15 (Fly-like putative transporter 1) (Flipt 1) PROTEIN FAMILY: Major facilitator superfamily, Organic cation transporter family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the major facilitator (TC 2.A.1) superfamily. Organic cation transporter (TC 2.A.1.19) family. FUNCTION: Organic zwitterion/cation transporter with apparent specificity for amino acids and their derivatives. Has low affinity for its substrates and may regulate their flux across the plasma membrane at high substrate concentrations. Bidirectionally transports carnitine and acetylcarnitine, possibly regulating their cytosolic abundance and further fatty acid catabolism via beta oxidation. Displays high transport activity toward zwitterionic substrates such as glycine betaine and diet-derived ergothioneine and carnosine. Can transport cations having an indole skeleton such as thiamine with lower efficiency. Does not transport agmatine. The transport mechanism, symport with sodium or facilitated diffusion allosterically regulated by sodium, remains to be elucidated (Probable). KEYWORDS: Alternative splicing;Amino-acid transport;Cell membrane;Glycoprotein;Ion transport;Membrane;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein " Q8IZK6,"PROTEIN NAMES: Mucolipin-2 (Transient receptor potential channel mucolipin 2) (TRPML2) PROTEIN FAMILY: Transient receptor family, Polycystin subfamily, MCOLN2 sub-subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the transient receptor (TC 1.A.4) family. Polycystin subfamily. MCOLN2 sub-subfamily. FUNCTION: Nonselective cation channel probably playing a role in the regulation of membrane trafficking events. Acts as a Ca(2+)-permeable cation channel with inwardly rectifying activity. May activate ARF6 and be involved in the trafficking of GPI-anchored cargo proteins to the cell surface via the ARF6-regulated recycling pathway. May play a role in immune processes. In adaptive immunity, TRPML2 and TRPML1 may play redundant roles in the function of the specialized lysosomes of B cells (By similarity). In the innate immune response, may play a role in the regulation of chemokine secretion and macrophage migration (By similarity). Through a possible and probably tissue-specific heteromerization with MCOLN1 may be at least in part involved in many lysosome-dependent cellular events. KEYWORDS: 3D-structure;Adaptive immunity;Alternative splicing;Calcium;Calcium channel;Calcium transport;Cell membrane;Disulfide bond;Endosome;Immunity;Innate immunity;Ion channel;Ion transport;Lysosome;Membrane;Protein transport;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Late endosome membrane ; Multi-pass membrane protein Lysosome membrane ; Multi-pass membrane protein Recycling endosome membrane ; Multi-pass membrane protein Note=Localizes to recycling endosomes in activated macrophages and microglia. " Q8J2V8,"PROTEIN NAMES: Pyranose 2-oxidase (P2Ox) (POD) (POx) (PROD) (Pyranose oxidase) (FAD-oxidoreductase) (Glucose 2-oxidase) (Pyranose:oxygen 2-oxidoreductase) PROTEIN FAMILY: GMC oxidoreductase family ORGANISM: Tricholoma matsutake (Matsutake mushroom) (Tricholoma nauseosum) SIMILARITY: Belongs to the GMC oxidoreductase family. FUNCTION: Catalyzes the oxidation of various aldopyranoses and disaccharides on carbon-2 to the corresponding 2-keto sugars concomitant with the reduction of O(2) to H(2)O(2). The preferred substrate is D-glucose which is converted to 2-dehydro-D-glucose. Acts also on D-xylose, L-sorbose, D-galactose and 1,5-anhydroglucitol, a diagnostic marker of diabetes mellitus. KEYWORDS: Direct protein sequencing;FAD;Flavoprotein;Oxidoreductase " Q8JHV9,PROTEIN NAMES: Baculoviral IAP repeat-containing protein 7-A (E3 ubiquitin-protein ligase EIAP-A) (Embryonic/Egg IAP) (xEIAP/XLX) (Inhibitor of apoptosis-like protein) (IAP-like protein) (RING-type E3 ubiquitin transferase EIAP-A) (XIAP homolog XLX) (XLX) PROTEIN FAMILY: IAP family ORGANISM: Xenopus laevis (African clawed frog) SIMILARITY: Belongs to the IAP family. FUNCTION: Weak apoptotic suppressor. Has E3 ubiquitin-protein ligase activity. Weak inhibitor of caspase activity. KEYWORDS: Apoptosis;Cytoplasm;Developmental protein;Metal-binding;Phosphoprotein;Protease inhibitor;Reference proteome;Repeat;Thiol protease inhibitor;Transferase;Ubl conjugation;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cytoplasm Q8JIT7,"PROTEIN NAMES: Forkhead activin signal transducer 3 (Fast-3) (xFAST-3) (Forkhead box protein H1b) (XFoxH1b) ORGANISM: Xenopus laevis (African clawed frog) FUNCTION: Transcriptional activator. Upon TGF-beta induction, forms a transcriptionally active complex with smad2 and smad4 called activin-responsive factor 2 (ARF2), which binds a site on the mix-B/mix.2 promoter called the activin response element (ARE). Binds to activated smads and the ARE with much higher affinity than foxh1/fast-1. Acts with foxh1/fast-1 to control the convergent extension movements of gastrulation. KEYWORDS: Activator;Developmental protein;DNA-binding;Gastrulation;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " Q8JZQ0,"PROTEIN NAMES: Macrophage colony-stimulating factor 1 (CSF-1) (MCSF) (Proteoglycan macrophage colony-stimulating factor) (PG-M-CSF) [Cleaved into: Processed macrophage colony-stimulating factor 1; Macrophage colony-stimulating factor 1 43 kDa subunit] ORGANISM: Rattus norvegicus (Rat) FUNCTION: Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of pro-inflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance (By similarity). KEYWORDS: Alternative splicing;Cell membrane;Cytokine;Disulfide bond;Glycoprotein;Growth factor;Immunity;Inflammatory response;Innate immunity;Membrane;Proteoglycan;Reference proteome;Secreted;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein.; SUBCELLULAR LOCATION: [Processed macrophage colony-stimulating factor 1]: Secreted, extracellular space " Q8K078,"PROTEIN NAMES: Solute carrier organic anion transporter family member 4A1 (Organic anion-transporting polypeptide E) (OATP-E) (Sodium-independent organic anion transporter E) (Solute carrier family 21 member 12) PROTEIN FAMILY: Organo anion transporter family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the organo anion transporter (TC 2.A.60) family. FUNCTION: Organic anion antiporter with apparent broad substrate specificity. Recognizes various substrates including thyroid hormones 3,3',5-triiodo-L-thyronine (T3), L-thyroxine (T4) and 3,3',5'-triiodo-L-thyronine (rT3), conjugated steroids such as estrone 3-sulfate and estradiol 17-beta glucuronide, bile acids such as taurocholate and prostanoids such as prostaglandin E2, likely operating in a tissue-specific manner (By similarity). May be involved in uptake of metabolites from the circulation into organs such as kidney, liver or placenta. Possibly drives the selective transport of thyroid hormones and estrogens coupled to an outward glutamate gradient across the microvillous membrane of the placenta (By similarity). The transport mechanism, its electrogenicity and potential tissue-specific counterions remain to be elucidated (Probable). KEYWORDS: Alternative splicing;Cell membrane;Disulfide bond;Glycoprotein;Ion transport;Membrane;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein " Q8K0L2,"PROTEIN NAMES: Ectonucleoside triphosphate diphosphohydrolase 8 (E-NTPDase 8) (NTPDase 8) (NTPDase8) PROTEIN FAMILY: GDA1/CD39 NTPase family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the GDA1/CD39 NTPase family. FUNCTION: Canalicular ectonucleoside NTPDase responsible for the main hepatic NTPDase activity. Ectonucleoside NTPDases catalyze the hydrolysis of gamma- and beta-phosphate residues of nucleotides, playing a central role in concentration of extracellular nucleotides. Has activity toward ATP, ADP, UTP and UDP, but not toward AMP. KEYWORDS: Alternative splicing;ATP-binding;Calcium;Cell membrane;Disulfide bond;Glycoprotein;Hydrolase;Magnesium;Membrane;Metal-binding;Nucleotide-binding;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein " Q8K1S6,"PROTEIN NAMES: Protein spire homolog 2 (Spir-2) PROTEIN FAMILY: Spire family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the spire family. FUNCTION: Acts as an actin nucleation factor, remains associated with the slow-growing pointed end of the new filament. Involved in intracellular vesicle transport along actin fibers, providing a novel link between actin cytoskeleton dynamics and intracellular transport. Required for asymmetric spindle positioning and asymmetric cell division during oocyte meiosis. Required for normal formation of the cleavage furrow and for polar body extrusion during female germ cell meiosis. Also acts in the nucleus: together with SPIRE1 and SPIRE2, promotes assembly of nuclear actin filaments in response to DNA damage in order to facilitate movement of chromatin and repair factors after DNA damage (By similarity). KEYWORDS: Actin-binding;Alternative splicing;Cell membrane;Cytoplasm;Cytoplasmic vesicle;Cytoskeleton;Membrane;Phosphoprotein;Protein transport;Reference proteome;Repeat;Transport SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton Cytoplasm, cytosol Cell membrane ; Peripheral membrane protein ; Cytoplasmic side Cytoplasmic vesicle membrane ; Peripheral membrane protein ; Cytoplasmic side Note=Detected at the cleavage furrow during asymmetric oocyte division and polar body extrusion. " Q8K202,"PROTEIN NAMES: DNA-directed RNA polymerase I subunit RPA49 (RNA polymerase I subunit A49) (DNA-directed RNA polymerase I subunit E) (RNA polymerase I-associated factor 1) (RNA polymerase I-associated factor 53) PROTEIN FAMILY: Eukaryotic RPA49/POLR1E RNA polymerase subunit family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the eukaryotic RPA49/POLR1E RNA polymerase subunit family. FUNCTION: Component of RNA polymerase I (Pol I), a DNA-dependent RNA polymerase which synthesizes ribosomal RNA precursors using the four ribonucleoside triphosphates as substrates. Appears to be involved in the formation of the initiation complex at the promoter by mediating the interaction between Pol I and UBTF/UBF. KEYWORDS: Acetylation;Alternative splicing;Direct protein sequencing;DNA-directed RNA polymerase;Nucleus;Phosphoprotein;Reference proteome;Transcription SUBCELLULAR LOCATION: Nucleus, nucleolus " Q8K2C7,"PROTEIN NAMES: Protein OS-9 PROTEIN FAMILY: OS-9 family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the OS-9 family. FUNCTION: Lectin which functions in endoplasmic reticulum (ER) quality control and ER-associated degradation (ERAD). May bind terminally misfolded non-glycosylated proteins as well as improperly folded glycoproteins, retain them in the ER, and possibly transfer them to the ubiquitination machinery and promote their degradation. Possible targets include TRPV4 (By similarity). KEYWORDS: Alternative splicing;Disulfide bond;Endoplasmic reticulum;Glycoprotein;Lectin;Reference proteome;Signal SUBCELLULAR LOCATION: Endoplasmic reticulum lumen " Q8K3P1,"PROTEIN NAMES: P2X purinoceptor 2 (P2X2) (ATP receptor) (Purinergic receptor) PROTEIN FAMILY: P2X receptor family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the P2X receptor family. FUNCTION: ATP-gated nonselective transmembrane cation channel permeable to potassium, sodium and calcium (By similarity). Activation by extracellular ATP induces a variety of cellular responses, such as excitatory postsynaptic responses in sensory neurons, neuromuscular junctions (NMJ) formation, hearing, perception of taste and peristalsis. In the inner ear, regulates sound transduction and auditory neurotransmission, outer hair cell electromotility, inner ear gap junctions, and K(+) recycling. Mediates synaptic transmission between neurons and from neurons to smooth muscle. KEYWORDS: Alternative splicing;ATP-binding;Cell membrane;Disulfide bond;Glycoprotein;Hearing;Ion channel;Ion transport;Ligand-gated ion channel;Membrane;Nucleotide-binding;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein " Q8K3X6,"PROTEIN NAMES: Ankyrin repeat and SAM domain-containing protein 4B (Harmonin-interacting ankyrin repeat-containing protein) (Harp) ORGANISM: Mus musculus (Mouse) FUNCTION: As part of the intermicrovillar adhesion complex/IMAC plays a role in epithelial brush border differentiation, controlling microvilli organization and length. Plays a role in assembly of the complex (By similarity). May play a role in cellular response to endoplasmic reticulum stress. KEYWORDS: 3D-structure;ANK repeat;Cell projection;Coiled coil;Differentiation;Reference proteome;Repeat SUBCELLULAR LOCATION: Cell projection, microvillus Note=Localizes at the tip of microvilli. May associate with endoplasmic reticulum membranes. " Q8K450,"PROTEIN NAMES: Sperm-associated antigen 16 protein (Pf20 protein homolog) ORGANISM: Mus musculus (Mouse) FUNCTION: Necessary for sperm flagellar function. Plays a role in motile ciliogenesis. May help to recruit STK36 to the cilium or apical surface of the cell to initiate subsequent steps of construction of the central pair apparatus of motile cilia. KEYWORDS: Alternative splicing;Cell projection;Cilium;Cilium biogenesis/degradation;Coiled coil;Cytoplasm;Cytoskeleton;Flagellum;Phosphoprotein;Reference proteome;Repeat;WD repeat SUBCELLULAR LOCATION: Cytoplasm Cytoplasm, cytoskeleton, cilium axoneme Cytoplasm, cytoskeleton, flagellum axoneme Cell projection, cilium, flagellum Note=Detected on the sperm flagellum. Detected in the central apparatus of the axoneme. Colocalizes with SPAG6 on microtubules. " Q8K4B2,"PROTEIN NAMES: Interleukin-1 receptor-associated kinase 3 (IRAK-3) (IL-1 receptor-associated kinase M) (IRAK-M) (Inactive IL-1 receptor-associated kinase 3) PROTEIN FAMILY: Protein kinase superfamily, TKL Ser/Thr protein kinase family, Pelle subfamily ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily. FUNCTION: Putative inactive protein kinase which regulates signaling downstream of immune receptors including IL1R and Toll-like receptors. Inhibits dissociation of IRAK1 and IRAK4 from the Toll-like receptor signaling complex by either inhibiting the phosphorylation of IRAK1 and IRAK4 or stabilizing the receptor complex. Upon IL33-induced lung inflammation, positively regulates expression of IL6, CSF3, CXCL2 and CCL5 mRNAs in dendritic cells. KEYWORDS: ATP-binding;Cytoplasm;Nucleotide-binding;Nucleus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Nucleus Note=In dendritic cells, translocates into the nucleus upon IL33 stimulation. " Q8K4R4,"PROTEIN NAMES: Cytoplasmic phosphatidylinositol transfer protein 1 (Mammalian rdgB homolog beta) (M-rdgB beta) (MrdgBbeta) (mM-rdgBbeta) (Retinal degeneration B homolog beta) (RdgBbeta) PROTEIN FAMILY: PtdIns transfer protein family, PI transfer class IIB subfamily ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the PtdIns transfer protein family. PI transfer class IIB subfamily. FUNCTION: [Isoform 1]: Catalyzes the transfer of phosphatidylinositol (PI) and phosphatidic acid (PA) between membranes (By similarity). Binds PA derived from the phospholipase D signaling pathway and among the cellular PA species, preferably binds to the C16:0/16:1 and C16:1/18:1 PA species (By similarity).; FUNCTION: [Isoform 2]: Specifically binds to phosphatidylinositol but not to other phospholipids and may play a role in the phosphoinositide-mediated signaling in the neural development. KEYWORDS: Alternative splicing;Cytoplasm;Lipid transport;Lipid-binding;Nucleus;Phosphoprotein;Reference proteome;Transport SUBCELLULAR LOCATION: [Isoform 1]: Cytoplasm.; SUBCELLULAR LOCATION: [Isoform 2]: Cytoplasm Nucleus " Q8K4T3,"PROTEIN NAMES: STE20-related kinase adapter protein beta (STRAD beta) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 2 protein homolog) (ILP-interacting protein homolog) (Polyploidy-associated protein kinase) (Pseudokinase ALS2CR2) PROTEIN FAMILY: Protein kinase superfamily, STE Ser/Thr protein kinase family, STE20 subfamily ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. FUNCTION: Pseudokinase which, in complex with CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta), binds to and activates STK11/LKB1. Adopts a closed conformation typical of active protein kinases and binds STK11/LKB1 as a pseudosubstrate, promoting conformational change of STK11/LKB1 in an active conformation (By similarity). KEYWORDS: Alternative splicing;ATP-binding;Cell cycle;Cytoplasm;Nucleotide-binding;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus. Cytoplasm. " Q8K4Y7,"PROTEIN NAMES: Soluble calcium-activated nucleotidase 1 (SCAN-1) (Apyrase homolog) PROTEIN FAMILY: Apyrase family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the apyrase family. FUNCTION: Calcium-dependent nucleotidase with a preference for UDP. The order of activity with different substrates is UDP > GDP > IDP >> UTP > CDP = GTP = ITP. Has very low activity towards ADP and even lower activity towards ATP. Does not hydrolyze AMP and GMP. Involved in proteoglycan synthesis (By similarity). KEYWORDS: Calcium;Endoplasmic reticulum;Glycoprotein;Golgi apparatus;Hydrolase;Membrane;Metal-binding;Reference proteome;Signal-anchor;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Single-pass type II membrane protein Golgi apparatus, Golgi stack membrane ; Single-pass type II membrane protein Note=Processed form: Secreted. " Q8KES3,"PROTEIN NAMES: Sepiapterin reductase (SPR) (cSR) PROTEIN FAMILY: Short-chain dehydrogenases/reductases (SDR) family ORGANISM: Chlorobaculum tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS) (Chlorobium tepidum) SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FUNCTION: Catalyzes the final reductions in tetra-hydrobiopterin biosynthesis to form 5,6,7,8-tetrahydrobiopterin. KEYWORDS: 3D-structure;Cytoplasm;Direct protein sequencing;NADP;Nucleotide-binding;Oxidoreductase;Reference proteome SUBCELLULAR LOCATION: Cytoplasm " Q8KLK7,"PROTEIN NAMES: (3,5-dihydroxyphenyl)acetyl-CoA 1,2-dioxygenase PROTEIN FAMILY: Enoyl-CoA hydratase/isomerase family ORGANISM: Streptomyces toyocaensis SIMILARITY: Belongs to the enoyl-CoA hydratase/isomerase family. FUNCTION: Involved in the biosynthesis of the nonproteinogenic amino acid monomer (S)-3,5-dihydroxyphenylglycine (Dpg) responsible of the production of vancomycin and teicoplanin antibiotics. Catalyzes the unusual conversion 3,5-dihydroxyphenylacetyl-CoA (DPA-CoA) to 3,5-dihydroxyphenylglyoxylate. DpgC performed a net four-electron oxidation of the benzylic carbon of DPA-CoA and the hydrolysis of the thioester bond to generate free CoA. DpgC has the ability to process a diverse range of substituted phenylacetyl-CoA substrates. KEYWORDS: 3D-structure;Antibiotic biosynthesis;Oxidoreductase;Reference proteome " Q8KNP3,"PROTEIN NAMES: Tubulin-like protein TubZ (FtsZ-like protein TubZ-Bt) PROTEIN FAMILY: FtsZ family, TubZ subfamily ORGANISM: Bacillus thuringiensis subsp. israelensis SIMILARITY: Belongs to the FtsZ family. TubZ subfamily. FUNCTION: A tubulin-like, filament forming GTPase; the motor component of the type III plasmid partition system which ensures correct segregation of the pBtoxis plasmid. Filaments may seed from the centromere-like site (tubC) when bound by DNA-binding protein TubR; the tubC-TubR complex stabilizes the TubZ filament. Filaments grow at the plus end and depolymerize at the minus end, a process called treadmilling. TubR-tubC complexes track the depolymerizing minus end of the filament, probably pulling plasmid within the cell. Required for pBtoxis plasmid replication/partition. Binds the TubR-tubC complex; GTP is not required for binding to TubR-tubC. TubZ alone does not bind DNA. Has a high GTPase activity in the presence of Mg(2+); in the presence of GTP assembles into dynamic filaments which upon polymerization bind almost exclusively GDP. Filament formation is cooperative, requiring a critical concentration. Formation occurs very quickly and is followed by disassembly as GTP is consumed. KEYWORDS: 3D-structure;Cytoplasm;GTP-binding;Hydrolase;Magnesium;Metal-binding;Nucleotide-binding;Plasmid;Plasmid partition SUBCELLULAR LOCATION: Cytoplasm Note=Forms long, dynamic filaments. MISCELLANEOUS: N-terminally tagged protein does not form filaments, C-terminally tagged protein does so, but not as well as untagged protein.; MISCELLANEOUS: The pBtoxis plasmid encodes all the major endotoxin proteins (Cyt1Aa, Cry4Aa, Cry4Ba, and Cry11Aa) responsible for the mosquito larvicidal activity of strain 4Q2." Q8KQL2,"PROTEIN NAMES: D-arabitol-phosphate dehydrogenase (APDH) PROTEIN FAMILY: Zinc-containing alcohol dehydrogenase family ORGANISM: Enterococcus avium (Streptococcus avium) SIMILARITY: Belongs to the zinc-containing alcohol dehydrogenase family. FUNCTION: Involved in the arabitol catabolism via the arabitol phosphate route. Catalyzes only the transformation of D-arabitol 1-phosphate (Arb1P) and D-arabitol 5-phosphate (Arb5P) into D-xylulose 5-phosphate (Xlu5P) and ribulose 5-phosphate, respectively. It can use both NAD and NADP. KEYWORDS: Manganese;Metal-binding;NAD;NADP;Oxidoreductase;Zinc " Q8KRU5,PROTEIN NAMES: Phospholipase D (PLD) [Cleaved into: Phospholipase D catalytic chain; Phospholipase D regulatory chain] PROTEIN FAMILY: PhoD family ORGANISM: Streptomyces chromofuscus SIMILARITY: Belongs to the PhoD family. FUNCTION: Catalyzes the hydrolysis of the ester bond between the phosphatidic acid and alcohol moieties of phospholipids. Can catalyze a transphosphatidylation reaction in the presence of high concentrations of primary alcohols. Has also phosphatase activity toward p-nitrophenyl phosphate. KEYWORDS: Allosteric enzyme;Direct protein sequencing;Hydrolase;Lipid degradation;Lipid metabolism;Secreted;Signal SUBCELLULAR LOCATION: Secreted Q8KZ94,"PROTEIN NAMES: Demethylrebeccamycin-D-glucose O-methyltransferase (Rebeccamycin O-methyltransferase) (Rebeccamycin sugar 4'-O-methyltransferase RebM) PROTEIN FAMILY: Methyltransferase superfamily ORGANISM: Lentzea aerocolonigenes (Lechevalieria aerocolonigenes) (Saccharothrix aerocolonigenes) SIMILARITY: Belongs to the methyltransferase superfamily. FUNCTION: Glycosyl O-methyltransferase that catalyzes the final step in the biosynthesis of rebeccamycin, an indolocarbazole alkaloid that inhibits topoisomerase 1. Has broad substrate specificity and functions as glycosyl O-methyltransferase on a number of rebeccamycin analogs. KEYWORDS: 3D-structure;Methyltransferase;S-adenosyl-L-methionine;Transferase " Q8L3A9,"PROTEIN NAMES: NADH-dependent phenylglyoxylate dehydrogenase subunit beta (Phenylglyoxylate:NAD oxidoreductase) (Phenylglyoxylate:acceptor oxidoreductase) ORGANISM: Aromatoleum evansii (Azoarcus evansii) FUNCTION: Involved in the anaerobic metabolism of phenylalanine and phenylacetate. Catalyzes the oxidative decarboxylation of phenylglyoxylate to benzoyl-CoA and CO(2). It can also react slowly with 2-oxo-3-methylbutanoate and use different electron acceptors such as benzyl viologen, methyl viologen, FAD or FMN, but NAD seems to be the physiological electron acceptor. Also catalyzes an isotope exchange between CO(2) and the carboxyl group which proves partial or complete reversibility of the oxidative decarboxylation reaction. KEYWORDS: 4Fe-4S;Direct protein sequencing;Iron;Iron-sulfur;Metal-binding;NAD;Oxidoreductase;Repeat " Q8L3X9,"PROTEIN NAMES: 3-oxoacyl-[acyl-carrier-protein] synthase, mitochondrial (Beta-ketoacyl-ACP synthase) (mtKAS) PROTEIN FAMILY: Thiolase-like superfamily, Beta-ketoacyl-ACP synthases family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the thiolase-like superfamily. Beta-ketoacyl-ACP synthases family. FUNCTION: Catalyzes all the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Able to elongate saturated acyl chains from 4 to at least 16 carbons. Uses malonyl-CoA but not acetyl-CoA as primer substrate. When expressed in a heterologous system, reveals a bimodal distribution of products, with peaks at C8 and C14-C16. The major product of the reaction (octanoyl-ACP) is required for the lipoylation of essential mitochondrial proteins. Required for mitochondrial fatty acid synthesis (mtFAS). MtFAS are essential for photorespiration and plant development, probably by influencing mitochondrial membrane lipid composition and other lipid metabolic pathways. PATHWAY: Lipid metabolism; fatty acid biosynthesis. KEYWORDS: 3D-structure;Acyltransferase;Fatty acid biosynthesis;Fatty acid metabolism;Lipid biosynthesis;Lipid metabolism;Mitochondrion;Reference proteome;Transferase;Transit peptide SUBCELLULAR LOCATION: Mitochondrion MISCELLANEOUS: Mitochondrial protein lipoylation in leaves does not exclusively depend on the lipoate biosynthesis by KAS and may occur independently of this pathway in roots." Q8L4M1,"PROTEIN NAMES: THO complex subunit 6 (AtTHO6) (Protein DWD HYPERSENSITIVE TO ABA 1) (WD repeat-containing protein DWA1) PROTEIN FAMILY: WD repeat THOC6 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the WD repeat THOC6 family. FUNCTION: Acts as a component of the THO subcomplex of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export.; FUNCTION: Component of the CUL4-RBX1-DDB1-DWA1/DWA2 E3 ubiquitin-protein ligase complex that acts as a negative regulator in abscisic acid (ABA) signaling. May function as the substrate recognition module within this complex leading to ABI5 degradation. Functionally redundant with DWA2. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Abscisic acid signaling pathway;mRNA processing;mRNA splicing;mRNA transport;Nucleus;Reference proteome;Repeat;RNA-binding;Transport;Ubl conjugation pathway;WD repeat SUBCELLULAR LOCATION: Nucleus " Q8L500,"PROTEIN NAMES: Two-component response regulator-like APRR9 (Pseudo-response regulator 9) PROTEIN FAMILY: ARR-like family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the ARR-like family. FUNCTION: Transcriptional repressor of CCA1 and LHY, and positive regulator of LWD1 and LWD2 expression. Controls photoperiodic flowering response and temperature compensation. Involved in the positive and negative feedback loops of the circadian clock. Expression of several members of the ARR-like family is controlled by circadian rhythm. Regulated at the transcriptional level by a corepressor complex consisting of ELF4, ELF3, and LUX. APRR9, APRR7, and APRR5 coordinately act on the upstream region of the target genes to repress their expression from noon until midnight. The particular coordinated sequential expression of APRR9, APRR7, APRR5, APRR3 and APPR1 result to circadian waves that may be at the basis of the endogenous circadian clock. KEYWORDS: Alternative splicing;Biological rhythms;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation;Two-component regulatory system SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: Regulated at the level of mRNA maturation and alternative splicing by SKIP and PRMT5. The expression of APRR9, APRR7, and APRR5 requires the presence of LWD1 and/or LWD2, indicating the existence of a positive feedback loop within the circadian clock." Q8L540,"PROTEIN NAMES: Thiol-disulfide oxidoreductase LTO1 (Protein LUMEN THIOL OXIDOREDUCTASE 1) (Vitamin K reductase) PROTEIN FAMILY: VKOR family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the VKOR family. FUNCTION: Thiol-disulfide oxidoreductase catalyzing disulfide bond formation of chloroplast proteins and involved in redox regulation and photosynthetic electron transport. Required for the assembly of photosystem II (PSII) through the formation of disulfide bond in PSBO, a subunit of the PSII oxygen-evolving complex in the thylakoid lumen. Involved in the formation of disulfide bonds in the lumenal protein FKBP13. In vitro, reduces phylloquinone (vitamin K1) and menaquinone (vitamin K2) to their respective quinol. Cannot reduce phylloquinone epoxide to phylloquinone. Plays an important role in regulating the thylakoid lumen redox. KEYWORDS: Chloroplast;Disulfide bond;Membrane;Oxidoreductase;Plastid;Quinone;Redox-active center;Reference proteome;Thylakoid;Transit peptide;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane ; Multi-pass membrane protein " Q8L5A7,"PROTEIN NAMES: Cytosolic sulfotransferase 15 (AtSOT15) (Sulfotransferase 2a) (AtST2a) PROTEIN FAMILY: Sulfotransferase 1 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the sulfotransferase 1 family. FUNCTION: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to specifically catalyze the sulfate conjugation of hydroxyjasmonates, with a preference for 12-hydroxyjasmonate over 11-hydroxyjasmonate. No activity with 12-hydroxyjasmonic acid methyl ester, cucurbic acid, 7-iso-cucurbic acid, 6-epi-cucurbic acid, 6-epi-7-iso-cucurbic acid and their methyl esters, prostaglandin E2, arachidonyl alcohol and 11-eicosenol. KEYWORDS: Cytoplasm;Reference proteome;Transferase SUBCELLULAR LOCATION: Cytoplasm " Q8L5U0,"PROTEIN NAMES: COP9 signalosome complex subunit 4 (AtS4) (Signalosome subunit 4) (Constitutive photomorphogenesis protein 8) (Protein FUSCA 4) PROTEIN FAMILY: CSN4 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the CSN4 family. FUNCTION: Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes such as photomorphogenesis and auxin and jasmonate responses. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF. It is involved in repression of photomorphogenesis in darkness by regulating the activity of COP1-containing Ubl ligase complexes. The complex is also required for degradation of IAA6 by regulating the activity of the Ubl ligase SCF-TIR complex. KEYWORDS: Acetylation;Cytoplasm;Developmental protein;Nucleus;Phytochrome signaling pathway;Reference proteome;Signalosome SUBCELLULAR LOCATION: Cytoplasm Nucleus " Q8L607,"PROTEIN NAMES: Short integuments 2, mitochondrial (DAR GTPase 1) PROTEIN FAMILY: TRAFAC class YlqF/YawG GTPase family, MTG1 subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the TRAFAC class YlqF/YawG GTPase family. MTG1 subfamily. FUNCTION: GTPase that may function in mitochondrial ribosome assembly (Probable). Involved in a variety of growth processes during vegetative development and promotes growth and cell division in the developing integuments. KEYWORDS: GTP-binding;Hydrolase;Mitochondrion;Nucleotide-binding;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Mitochondrion " Q8L6Y4,"PROTEIN NAMES: Polycomb group protein EMBRYONIC FLOWER 2 PROTEIN FAMILY: VEFS (VRN2-EMF2-FIS2-SU(Z)12) family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the VEFS (VRN2-EMF2-FIS2-SU(Z)12) family. FUNCTION: Polycomb group (PcG) protein. Involved in flowering processes by repressing unknown target genes and preventing reproductive development. Participates in polycomb group (PcG) protein complex-mediated (probably in complex with EMF1) silencing of the flower homeotic genes AGAMOUS (AG), PISTILLATA (PI), and APETALA3 (AP3), as well as of some regulatory genes such as ABSCISIC ACID INSENSITIVE3 (ABI3), LONG VEGETATIVE PHASE1 (LOV1), and FLOWERING LOCUS C (FLC) during vegetative development, by mediating trimethylation of histone 3 lysine 27 on the AG chromatin (H3K27me3). PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development. PcG proteins are not required to initiate repression, but to maintain it during later stages of development. They probably act via the methylation of histones, rendering chromatin heritably changed in its expressibility. KEYWORDS: Alternative splicing;Chromatin regulator;Developmental protein;Differentiation;Flowering;Metal-binding;Nucleus;Reference proteome;Repressor;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus " Q8L707,"PROTEIN NAMES: Beta-1,4-xylosyltransferase IRX14 (Protein IRREGULAR XYLEM 14) (Xylan xylosyltransferase IRX14) PROTEIN FAMILY: Glycosyltransferase 43 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the glycosyltransferase 43 family. FUNCTION: Involved in the synthesis of the hemicellulose glucuronoxylan, a major component of secondary cell walls. Involved in the elongation of glucuronoxylan xylosyl backbone. Xylan xylosyltransferase that acts cooperatively with IRX9 to achieve the successive addition of xylosyl residues during xylan backbone elongation. Required for the proper composition and structural properties of released seed coat mucilage. Required for the production of highly branched xylan polymers in seed coat mucilage. Xylan with xylose side chains seems to be necessary for pectin attachment to the seed surface. Together with MUCI70, required for xylan and pectin synthesis in seed coat epidermal (SCE) cells. KEYWORDS: Cell wall biogenesis/degradation;Glycoprotein;Glycosyltransferase;Golgi apparatus;Membrane;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus membrane ; Single-pass type II membrane protein " Q8L7F9,"PROTEIN NAMES: Beta-1,3-galactosyltransferase GALT1 (Beta-1,3-galactosyltransferase 15) (Galactosyltransferase 1) PROTEIN FAMILY: Glycosyltransferase 31 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the glycosyltransferase 31 family. FUNCTION: Beta-1,3-galactosyltransferase that transfers galactose from UDP-galactose to substrates with a terminal beta-N-acetylglucosamine (beta-GlcNAc) residue. Involved in the biosynthesis of N-glycans containing Lewis a structures (with the combination of FUT13). PATHWAY: Protein modification; protein glycosylation. KEYWORDS: Glycoprotein;Glycosyltransferase;Golgi apparatus;Manganese;Membrane;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus membrane ; Single-pass type II membrane protein " Q8L7Q7,PROTEIN NAMES: Probable pectinesterase/pectinesterase inhibitor 64 [Includes: Pectinesterase inhibitor 64 (Pectin methylesterase inhibitor 64); Pectinesterase 64 (PE 64) (Pectin methylesterase 64) (AtPME64)] PROTEIN FAMILY: PMEI family; Pectinesterase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: In the N-terminal section; belongs to the PMEI family.; SIMILARITY: In the C-terminal section; belongs to the pectinesterase family. FUNCTION: Acts in the modification of cell walls via demethylesterification of cell wall pectin. PATHWAY: Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5. KEYWORDS: Aspartyl esterase;Disulfide bond;Glycoprotein;Hydrolase;Membrane;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Single-pass membrane protein MISCELLANEOUS: The PMEI region may act as an autoinhibitory domain and prevent untimely PME activity during transport. Q8L8A6,PROTEIN NAMES: Growth-regulating factor 5 (AtGRF5) (Transcription activator GRF5) PROTEIN FAMILY: GRF family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the GRF family. FUNCTION: Transcription activator that plays a role in the regulation of cell expansion in leaf and cotyledons tissues. Acts together with GIF1 for the development of appropriate leaf size and shape through the promotion and/or maintenance of cell proliferation activity in leaf primordia. KEYWORDS: Activator;Alternative splicing;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: [Isoform 2]: May be due to intron retention. Q8L9C4,"PROTEIN NAMES: Very-long-chain 3-oxoacyl-CoA reductase 1 (Beta-ketoacyl reductase 1) (AtKCR1) (Protein GLOSSY 8) (gl8At) PROTEIN FAMILY: Short-chain dehydrogenases/reductases (SDR) family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FUNCTION: Beta-ketoacyl-coenzyme A reductase required for the elongation of fatty acids precursors of sphingolipids, triacylglycerols, cuticular waxes and suberin. Responsible for the first reduction step in very long-chain fatty acids (VLCFAs) synthesis. Decreased expression of KCR1 (RNAi) leads to plants with fused vegetative and reproductive organs, and abnormal trichome, epidermal cell and root morphology. Cannot be complemented by KCR2. PATHWAY: Lipid metabolism; fatty acid biosynthesis. KEYWORDS: Endoplasmic reticulum;Fatty acid biosynthesis;Fatty acid metabolism;Lipid biosynthesis;Lipid metabolism;Membrane;NADP;Oxidoreductase;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Single-pass membrane protein " Q8LBL1,"PROTEIN NAMES: Two-pore potassium channel 1 (AtTPK1) (Calcium-activated outward-rectifying potassium channel 1) (AtKCO1) PROTEIN FAMILY: Two pore domain potassium channel family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the two pore domain potassium channel (TC 1.A.1.7) family. FUNCTION: Voltage-independent, large conductance and potassium-selective tonoplast ion channel. Regulated by cytoplasmic calcium and pH. Does not mediate slow-vacuolar (SV) ionic currents, but essential to establish VK currents. Has some permeability for Rb(+) and NH(4)(+), but none for Na(+), Cs(+) or Li(+). Involved in intracellular K(+) redistribution and/or K(+) retranslocation between different tissues. KEYWORDS: Calcium;Ion channel;Ion transport;Membrane;Metal-binding;Phosphoprotein;Potassium;Potassium channel;Potassium transport;Reference proteome;Repeat;Transmembrane;Transmembrane helix;Transport;Vacuole SUBCELLULAR LOCATION: Vacuole membrane ; Multi-pass membrane protein Note=Tonoplast. MISCELLANEOUS: 14-3-3 protein binding is not involved in endoplasmic reticulum export and tonoplast targeting." Q8LBP4,"PROTEIN NAMES: Inner membrane protein ALBINO3, chloroplastic PROTEIN FAMILY: OXA1/ALB3/YidC family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the OXA1/ALB3/YidC (TC 2.A.9.2) family. FUNCTION: Required for the insertion of some light harvesting chlorophyll-binding proteins (LHCP) into the chloroplast thylakoid membrane. Required for the insertion of LHCB1, LHCB4.1 and LHCB5 proteins into thylakoid membrane, while it is not required for insertion of proteins PsbX, PsbW and PsbY. KEYWORDS: 3D-structure;Alternative splicing;Chloroplast;Membrane;Plastid;Reference proteome;Thylakoid;Transit peptide;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane ; Multi-pass membrane protein " Q8LDC8,"PROTEIN NAMES: Ethylene-responsive transcription factor 1B (AtERF1B) (Ethylene-responsive element-binding factor 1B) (EREBP-1B) PROTEIN FAMILY: AP2/ERF transcription factor family, ERF subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the AP2/ERF transcription factor family. ERF subfamily. FUNCTION: Acts as a transcriptional activator. Binds to the GCC-box pathogenesis-related promoter element. Involved in the regulation of gene expression during the plant development, and/or mediated by stress factors and by components of stress signal transduction pathways. Seems to be a key integrator of ethylene and jasmonate signals in the regulation of ethylene/jasmonate-dependent defenses. Can mediate resistance to necrotizing fungi (Botrytis cinerea and Plectosphaerella cucumerina) and to soil borne fungi (Fusarium oxysporum conglutinans and Fusiarium oxysporum lycopersici), but probably not to necrotizing bacteria (Pseudomonas syringae tomato). KEYWORDS: Activator;DNA-binding;Ethylene signaling pathway;Nucleus;Plant defense;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " Q8LDU4,"PROTEIN NAMES: Red chlorophyll catabolite reductase, chloroplastic (AtRCCR) (RCC reductase) (Accelerated cell death protein 2) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Catalyzes the key reaction of chlorophyll catabolism, porphyrin macrocycle cleavage of pheophorbide a (pheide a) to a primary fluorescent catabolite (pFCC). Works in a two-step reaction with pheophorbide a oxygenase (PaO) by reducing the C20/C1 double bond of the intermediate, RCC. Belongs to the chlorophyll catabolic enzymes (CCEs). PATHWAY: Porphyrin-containing compound metabolism; chlorophyll degradation. KEYWORDS: 3D-structure;Chlorophyll catabolism;Chloroplast;Coiled coil;Membrane;NADP;Oxidoreductase;Plastid;Reference proteome;Thylakoid;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast stroma. Plastid, chloroplast thylakoid membrane. Note=And a low amount in mitochondria of 7-day-old seedlings. MISCELLANEOUS: The absence of light completely suppresses cell death in acd2 mutants." Q8LEG1,"PROTEIN NAMES: Transcription factor RSL3 (Basic helix-loop-helix protein 54) (AtbHLH54) (bHLH 54) (ROOT HAIR DEFECTIVE 6-LIKE 4) (Protein RHD SIX-LIKE 4) (Transcription factor EN 114) (Transcription factor bHLH54) (bHLH transcription factor bHLH054) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Transcription factor involved in the regulation of root hair elongation. Is sufficient to promote postmitotic cell growth in root-hair cells and is a direct transcriptional target of RHD6 and RSL1. Involved in the regulation of root hair elongation in response to low phosphate. Controls root hair cell growth by regulating the expression of genes encoding proteins involved in cell signaling, cell wall modification and secretion. KEYWORDS: DNA-binding;Nucleus;Reference proteome;Transcription;Transcription regulation;Ubl conjugation SUBCELLULAR LOCATION: Nucleus " Q8LEK2,"PROTEIN NAMES: Golgi apparatus membrane protein-like protein ECHIDNA PROTEIN FAMILY: TVP23 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the TVP23 family. FUNCTION: Mediates trans-Golgi-network trafficking and cell elongation. Required for keeping the appropriate balance between secretory trafficking and vacuolar targeting of a subset of proteins. The ECH/YIP4 complex is involved in the modulation of the trans-Golgi network (TGN)-mediated trafficking of some proteins and cell wall components (e.g. pectin and hemicellulose) to the cell wall in dark-grown hypocotyls and in secretory cells of the seed coat. KEYWORDS: Acetylation;Endosome;Golgi apparatus;Membrane;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus, trans-Golgi network membrane ; Multi-pass membrane protein Early endosome membrane; Multi-pass membrane protein. " Q8LEU3,"PROTEIN NAMES: Chlorophyll(ide) b reductase NOL, chloroplastic (Protein NON-YELLOW COLORING 1-LIKE) (AtNOL) (Protein NYC1-LIKE) (Short-chain dehydrogenase/reductase NOL) PROTEIN FAMILY: Short-chain dehydrogenases/reductases (SDR) family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FUNCTION: Required for chlorophyll b degradation. Chlorophyll b, chlorophyllide b, pheophorbide b and pheophytin b can be used as substrates. Belongs to the chlorophyll catabolic enzymes (CCEs). KEYWORDS: Chlorophyll catabolism;Chloroplast;Membrane;NAD;Oxidoreductase;Plastid;Reference proteome;Thylakoid;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane ; Peripheral membrane protein MISCELLANEOUS: Chlorophyll b reductase activity detected in vitro with a recombinant protein produced in a heterologous system. Able to act on the substrate within the protein-chlorophyll LHCII complex." Q8LFH5,"PROTEIN NAMES: Bidirectional sugar transporter SWEET8 (AtSWEET8) (Protein RUPTURED POLLEN GRAIN 1) (Protein SUGARS WILL EVENTUALLY BE EXPORTED TRANSPORTERS 8) PROTEIN FAMILY: SWEET sugar transporter family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the SWEET sugar transporter family. FUNCTION: Mediates both low-affinity uptake and efflux of sugar across the plasma membrane. Required, in pollen, for microspore cell integrity and primexine pattern formation. KEYWORDS: Alternative splicing;Cell membrane;Membrane;Reference proteome;Repeat;Sugar transport;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein MISCELLANEOUS: [Isoform 2]: Not detected in inflorescence." Q8LG98,"PROTEIN NAMES: OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 1 (OTU domain-containing protein 1) (Deubiquitinating enzyme OTU1) PROTEIN FAMILY: Peptidase C65 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the peptidase C65 family. FUNCTION: Hydrolase that can remove conjugated ubiquitin from proteins in vitro and may therefore play an important regulatory role at the level of protein turnover by preventing degradation. Cysteine protease with a preference for Met-1 and 'Lys-48' over 'Lys-63'-linked ubiquitin (UB) tetramers (e.g. Ub2, Ub3 and Ub4) as substrates. KEYWORDS: Hydrolase;Protease;Reference proteome;Thiol protease;Ubl conjugation pathway " Q8LGF7,"PROTEIN NAMES: Protein PEROXIN-4 (AtPEX4) (E2 ubiquitin-conjugating enzyme 21) (Probable ubiquitin-conjugating enzyme E2 21) (Ubiquitin carrier protein 21) PROTEIN FAMILY: Ubiquitin-conjugating enzyme family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the ubiquitin-conjugating enzyme family. FUNCTION: Required for peroxisome biogenesis. Necessary for the developmental elimination of obsolete peroxisome matrix proteins. May be involved in the ubiquitination of PEX5, targeting it for recycling. Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: 3D-structure;ATP-binding;Membrane;Nucleotide-binding;Peroxisome;Peroxisome biogenesis;Protein transport;Reference proteome;Transferase;Transport;Ubl conjugation pathway SUBCELLULAR LOCATION: Peroxisome membrane ; Peripheral membrane protein " Q8LGI3,"PROTEIN NAMES: Actin-related protein 2/3 complex subunit 2A (Actin-related protein C2A) (Arp2/3 complex 34 kDa subunit) (p34-ARC) (Protein DISTORTED TRICHOMES 2) PROTEIN FAMILY: ARPC2 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the ARPC2 family. FUNCTION: Functions as actin-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the mother actin filament (By similarity). Arp2/3 complex plays a critical role in the control of cell morphogenesis via the modulation of cell polarity development. KEYWORDS: Actin-binding;Cell projection;Cytoplasm;Cytoskeleton;Reference proteome SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton Cell projection " Q8LGU6,"PROTEIN NAMES: Cell division cycle protein 27 homolog B (CDC27 homolog B) (Protein HOBBIT) PROTEIN FAMILY: APC3/CDC27 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the APC3/CDC27 family. FUNCTION: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin-protein ligase complex that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex controls several key steps in the cell cycle by mediating ubiquitination and subsequent degradation of target proteins such as cyclins. The APC/C complex is required for the female gametophyte development and is involved in several aspect of development by controlling cell division and cell elongation. Involved in the control of endoreduplication. Functionally redundant with CDC27A in the control of gametophyte development. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Cell cycle;Cell division;Mitosis;Nucleus;Reference proteome;Repeat;TPR repeat;Ubl conjugation pathway SUBCELLULAR LOCATION: Nucleus " Q8LGZ9,"PROTEIN NAMES: Gibberellin 2-beta-dioxygenase 5 (Gibberellin 2-beta-hydroxylase 5) (Gibberellin 2-oxidase 5) (GA 2-oxidase 5) (OsGA2ox5) PROTEIN FAMILY: Iron/ascorbate-dependent oxidoreductase family, GA2OX subfamily ORGANISM: Oryza sativa subsp. japonica (Rice) SIMILARITY: Belongs to the iron/ascorbate-dependent oxidoreductase family. GA2OX subfamily. FUNCTION: Catalyzes the 2-beta-hydroxylation of several biologically active gibberellins (GAs), leading to the homeostatic regulation of their endogenous level. Catabolism of GAs plays a central role in plant development. In vitro, converts GA12 and GA53 to the corresponding 2-beta-hydroxylated products GA110 and GA97, respectively. PATHWAY: Plant hormone biosynthesis; gibberellin biosynthesis. KEYWORDS: Cytoplasm;Dioxygenase;Iron;Metal-binding;Nucleus;Oxidoreductase;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Nucleus MISCELLANEOUS: Plant overexpressing GA2OX5 exhibit an extremely dwarf phenotype." Q8LPN3,"PROTEIN NAMES: Aminodeoxychorismate synthase, chloroplastic (ADC synthase) (P-aminobenzoic acid synthase) (PABA synthase) (Para-aminobenzoate synthase) (Protein EMBRYO DEFFECTIVE 1997) PROTEIN FAMILY: Anthranilate synthase component I family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: In the C-terminal section; belongs to the anthranilate synthase component I family. FUNCTION: Bifunctional enzyme that catalyzes the biosynthesis of 4-amino-4-deoxychorismate (ADC) from chorismate and glutamine. In the first step, a glutamine amidotransferase generates ammonia that is channelled between the binding sites of glutamine and chorismate and used along with chorismate in the second step, catalyzed by aminodeoxychorismate synthase, to produce ADC. Required for the synthesis of 4-aminobenzoate (PABA), an important component in tetrahydrofolate biosynthesis. Does not possess ADC lyase activity. PATHWAY: Cofactor biosynthesis; tetrahydrofolate biosynthesis; 4-aminobenzoate from chorismate: step 1/2. KEYWORDS: Chloroplast;Folate biosynthesis;Glutamine amidotransferase;Multifunctional enzyme;Plastid;Reference proteome;Transferase;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast " Q8MKW7,"PROTEIN NAMES: Ribonuclease Z, mitochondrial (RNase Z) (Juvenile hormone-inducible protein 1) (dRNAse Z) (tRNA 3 endonuclease) (DmeZ) (tRNase Z) PROTEIN FAMILY: RNase Z family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the RNase Z family. FUNCTION: Zinc phosphodiesterase, which displays some tRNA 3'-processing endonuclease activity of nuclear and mitochondrial pre-tRNA. Probably involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA. May participate in tRNA processing in the developing embryo. KEYWORDS: Developmental protein;Endonuclease;Hydrolase;Metal-binding;Mitochondrion;Nuclease;Nucleus;Reference proteome;Transit peptide;tRNA processing;Zinc SUBCELLULAR LOCATION: Nucleus Mitochondrion MISCELLANEOUS: The dual subcellular location may be due to some alternative splicing and/or initiation that changes the initiator methionine." Q8MNT9,"PROTEIN NAMES: DNA N6-methyl adenine demethylase (N6-methyl adenine demethylase 1) PROTEIN FAMILY: AlkB family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the alkB family. FUNCTION: Dioxygenase that specifically demethylates DNA methylated on the 6th position of adenine (N(6)-methyladenosine) DNA. N(6)-methyladenosine (m6A) DNA is involved in epigenetic transgenerational inheritance. Plays an essential role in DNA replication and repair in the germline during meiosis. Binds to components of the DNA replication machinery such as top-2, and directs their localization to DNA to control DNA replication. KEYWORDS: 3D-structure;Alternative splicing;Dioxygenase;Iron;Metal-binding;Nucleus;Oxidoreductase;Reference proteome SUBCELLULAR LOCATION: Nucleus Note=May co-localize with top-2 on DNA. " Q8MQ70,"PROTEIN NAMES: Homeodomain-interacting protein kinase 1 PROTEIN FAMILY: Protein kinase superfamily, CMGC Ser/Thr protein kinase family, HIPK subfamily ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. HIPK subfamily. FUNCTION: Serine/threonine-protein kinase required in the somatic gonadal cells to regulate germline proliferation during larval development and in adulthood. Plays a role in the development/differentiation of gonadal distal tip cells. Required for normal lifespan in a pha-4 and mxl-2-dependent manner. Also contributes to survival following heat or oxidative stress. Prevents sumoylation and inactivation of heat shock transcription factor hsf-1 which enhances hsf-1-dependent transcriptional induction of chaperones in response to heat shock. Also required for hormetic extension of longevity in response to heat stress. Provides protection against polyglutamine aggregate formation and associated locomotory toxicity. Also contributes to longevity by promoting autophagy under nutrient stress conditions through induction of autophagosome formation and autophagy gene expression. KEYWORDS: Alternative splicing;ATP-binding;Autophagy;Developmental protein;Kinase;Nucleotide-binding;Nucleus;Reference proteome;Serine/threonine-protein kinase;Transferase SUBCELLULAR LOCATION: Nucleus Note=Localizes to nuclear moving puncta. Puncta number and intensity decrease in the adult. In some large cells, a nuclear speckle pattern can be detected. " Q8MRC9,"PROTEIN NAMES: Putative polypeptide N-acetylgalactosaminyltransferase 9 (pp-GaNTase 9) (Protein-UDP acetylgalactosaminyltransferase 9) (UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 9) PROTEIN FAMILY: Glycosyltransferase 2 family, GalNAc-T subfamily ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily. FUNCTION: Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. It can both act as a peptide transferase that transfers GalNAc onto unmodified peptide substrates, and as a glycopeptide transferase that requires the prior addition of a GalNAc on a peptide before adding additional GalNAc moieties.; FUNCTION: [Isoform A]: N-acetylgalactosaminyltransferase which preferentially O-glycosylates negatively charge substrates. O-glycosylates mucin-like protein Sgs3 in the salivary gland but to a lesser extent than isoform B. By regulating the O-glycosylation of secretory cargo proteins plays a role in the morphology and maturation of salivary gland secretory granules.; FUNCTION: [Isoform B]: N-acetylgalactosaminyltransferase which preferentially O-glycosylates positively charge substrates. O-glycosylates mucin-like protein Sgs3 in the salivary gland. By regulating the O-glycosylation of secretory cargo proteins, plays a role in the morphology and maturation of salivary gland secretory granules. PATHWAY: Protein modification; protein glycosylation. KEYWORDS: 3D-structure;Alternative splicing;Disulfide bond;Glycoprotein;Glycosyltransferase;Golgi apparatus;Lectin;Manganese;Membrane;Metal-binding;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: [Isoform A]: Golgi apparatus membrane ; Single-pass type II membrane protein.; SUBCELLULAR LOCATION: [Isoform B]: Golgi apparatus membrane ; Single-pass type II membrane protein " Q8MUJ1,"PROTEIN NAMES: Protein eiger [Cleaved into: Protein eiger, membrane form; Protein eiger, soluble form] PROTEIN FAMILY: Tumor necrosis factor family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the tumor necrosis factor family. FUNCTION: Cytokine which acts as a ligand for wgn. Also acts as a ligand for grnd. Induces apoptosis by triggering JNK signaling. Required for JNK-dependent non-autonomous apoptosis through release from apoptotic cells and activation of apoptosis in neighboring cells. Required for JNK-independent damage-induced apoptosis in the embryonic central nervous system through regulation of the pro-apoptotic gene hid. Involved in the innate immune response to extracellular pathogens. Plays a role in the melanization immune response through its involvement in the rupture of crystal cells and subsequent release of prophenoloxidase. Following UV-induced epidermal damage, released from apoptotic epidermal cells, binds to the wgn receptor on nociceptive sensory neurons and plays a role in development of thermal allodynia, a responsiveness to subthreshold thermal stimuli which are not normally perceived as noxious. Involved in glial cell division induced by neuronal programmed cell death and injury. Has tumor suppressor activity and eliminates oncogenic cells from epithelia, thereby maintaining epithelial integrity. KEYWORDS: 3D-structure;Alternative splicing;Apoptosis;Cell membrane;Coiled coil;Cytokine;Direct protein sequencing;Disulfide bond;Glycoprotein;Immunity;Innate immunity;Membrane;Reference proteome;Secreted;Signal-anchor;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: [Protein eiger, membrane form]: Cell membrane ; Single-pass type II membrane protein.; SUBCELLULAR LOCATION: [Protein eiger, soluble form]: Secreted " Q8MVS5,"PROTEIN NAMES: Polypeptide N-acetylgalactosaminyltransferase 35A (Protein l(2)35Aa) (Protein-UDP acetylgalactosaminyltransferase 35A) (UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 35A) (pp-GaNTase 35A) (dGalNAc-T1) PROTEIN FAMILY: Glycosyltransferase 2 family, GalNAc-T subfamily ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily. FUNCTION: Polypeptide N-acetylgalactosaminyltransferases catalyze the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Displays the same enzyme activity toward MUC1, MUC4, and EA2. Not involved in glycosylation of erythropoietin (EPO). It can both act as a peptide transferase that transfers GalNAc onto unmodified peptide substrates, and as a glycopeptide transferase that requires the prior addition of a GalNAc on a peptide before adding additional GalNAc moieties. Protein modification by this enzyme might be important for cytokinesis and tube formation during embryogenesis. Together with Pgant3, regulates integrin levels and activity-dependent integrin signaling at the synapse in neurons and muscles. PATHWAY: Protein modification; protein glycosylation. KEYWORDS: Disulfide bond;Glycoprotein;Glycosyltransferase;Golgi apparatus;Lectin;Manganese;Membrane;Metal-binding;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus membrane ; Single-pass type II membrane protein MISCELLANEOUS: The human ortholog GALNT11 (AC Q8NCW6) is not able to rescue lethality caused by the SF32 mutation." Q8MXY0,"PROTEIN NAMES: Ammonium transporter 3 PROTEIN FAMILY: Ammonia transporter channel family ORGANISM: Dictyostelium discoideum (Social amoeba) SIMILARITY: Belongs to the ammonia transporter channel (TC 1.A.11.2) family. FUNCTION: Ammonium transporter that mediates the import of ammonium in prespore cells. Controls ammonium homeostasis during growth and development. Ammonium has been shown to function as a morphogen at multiple steps during the development. May function as an ammonia sensor that relays information concerning ammonia concentrations to the signaling pathway involved in the slug versus culmination choice and regulates prestalk gene expression. KEYWORDS: Ammonia transport;Cell membrane;Cytoplasmic vesicle;Endosome;Membrane;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Endosome membrane ; Multi-pass membrane protein Cytoplasmic vesicle, phagosome membrane ; Multi-pass membrane protein " Q8MY12,"PROTEIN NAMES: Myosin heavy chain kinase C (MHCK-C) PROTEIN FAMILY: Protein kinase superfamily, Alpha-type protein kinase family, ALPK subfamily ORGANISM: Dictyostelium discoideum (Social amoeba) SIMILARITY: Belongs to the protein kinase superfamily. Alpha-type protein kinase family. ALPK subfamily. FUNCTION: Phosphorylates threonine at 'Thr-1823', 'Thr-1833' and 'Thr-2029' in the C-terminal tail region of myosin II heavy chain (mhcA) (By similarity). This phosphorylation is critical in actin-activated ATPase activity of the myosin and regulating the assembly and disassembly of myosin II filament. In vitro, catalytic domain phosphorylates mhcA, myelin basic protein, myosin regulatory light chain, casein and caldesmon. Drives the disassembly of myosin II filaments for efficient cytokinesis and recycling of myosin II that occurs during late cytokinesis. Can be activated in vitro by autophosphorylation. KEYWORDS: ATP-binding;Cytoplasm;Kinase;Membrane;Nucleotide-binding;Phosphoprotein;Reference proteome;Repeat;Serine/threonine-protein kinase;Transferase;WD repeat SUBCELLULAR LOCATION: Cytoplasm, cell cortex. Membrane. Cleavage furrow. Note=Localizes to the cortex of interphase cells, cleavage furrow of mitotic cells (at the late stage of cytokinesis), and posterior side of both migrating cells and daughter cells beginning to separate at the very late stage of cytokinesis. Localization is myosin II-dependent. Occasionally displays transient enrichment in pseudopodial extensions as well. " Q8MZM7,"PROTEIN NAMES: CLIP domain-containing serine protease B8 [Cleaved into: CLIP domain-containing serine protease B8 light chain; CLIP domain-containing serine protease B8 heavy chain] PROTEIN FAMILY: Peptidase S1 family, CLIP subfamily ORGANISM: Anopheles gambiae (African malaria mosquito) SIMILARITY: Belongs to the peptidase S1 family. CLIP subfamily. FUNCTION: Serine protease that functions in the melanization-mediated immune response. Preferentially, cleaves substrates with an arginine at the P1 site. May be involved in the activation of the prophenoloxidase cascade upstream of CLIPB9; does not cleave prophenoloxidase. KEYWORDS: Disulfide bond;Glycoprotein;Hydrolase;Immunity;Innate immunity;Protease;Reference proteome;Secreted;Serine protease;Signal;Zymogen SUBCELLULAR LOCATION: Secreted " Q8N0V5,"PROTEIN NAMES: N-acetyllactosaminide beta-1,6-N-acetylglucosaminyl-transferase (N-acetylglucosaminyltransferase) (I-branching enzyme) (IGNT) PROTEIN FAMILY: Glycosyltransferase 14 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the glycosyltransferase 14 family. FUNCTION: Branching enzyme that converts linear into branched poly-N-acetyllactosaminoglycans. Introduces the blood group I antigen during embryonic development. It is closely associated with the development and maturation of erythroid cells.; FUNCTION: [Isoform C]: Determines the expression of the blood group I antigen in erythrocytes. PATHWAY: Protein modification; protein glycosylation. KEYWORDS: Alternative splicing;Cataract;Disease variant;Glycoprotein;Glycosyltransferase;Golgi apparatus;Membrane;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus membrane ; Single-pass type II membrane protein " Q8N0X4,"PROTEIN NAMES: Citramalyl-CoA lyase, mitochondrial ((3S)-malyl-CoA thioesterase) (Beta-methylmalate synthase) (Citrate lyase subunit beta-like protein) (Citrate lyase beta-like) (Malate synthase) PROTEIN FAMILY: HpcH/HpaI aldolase family, Citrate lyase beta subunit-like subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the HpcH/HpaI aldolase family. Citrate lyase beta subunit-like subfamily. FUNCTION: Mitochondrial citramalyl-CoA lyase indirectly involved in the vitamin B12 metabolism. Converts citramalyl-CoA into acetyl-CoA and pyruvate in the C5-dicarboxylate catabolism pathway. The C5-dicarboxylate catabolism pathway is required to detoxify itaconate, a vitamin B12-poisoning metabolite. Also acts as a malate synthase in vitro, converting glyoxylate and acetyl-CoA to malate. Also displays malyl-CoA thioesterase activity. Also acts as a beta-methylmalate synthase in vitro, by mediating conversion of glyoxylate and propionyl-CoA to beta-methylmalate. Also has very weak citramalate synthase activity in vitro. KEYWORDS: 3D-structure;Acetylation;Alternative splicing;Hydrolase;Lyase;Magnesium;Metal-binding;Mitochondrion;Reference proteome;Transferase;Transit peptide SUBCELLULAR LOCATION: Mitochondrion " Q8N114,PROTEIN NAMES: Protein shisa-5 (Putative NF-kappa-B-activating protein 120) (Scotin) PROTEIN FAMILY: Shisa family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the shisa family. FUNCTION: Can induce apoptosis in a caspase-dependent manner and plays a role in p53/TP53-dependent apoptosis. KEYWORDS: Alternative splicing;Apoptosis;Endoplasmic reticulum;Membrane;Nucleus;Reference proteome;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Single-pass type I membrane protein Nucleus membrane Q8N1E6,PROTEIN NAMES: F-box/LRR-repeat protein 14 (F-box and leucine-rich repeat protein 14) ORGANISM: Homo sapiens (Human) FUNCTION: Substrate-recognition component of some SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin-protein ligase complexes. The SCF(FBXL14) complex acts by mediating ubiquitination and subsequent degradation of SNAI1. KEYWORDS: Cytoplasm;Leucine-rich repeat;Reference proteome;Repeat;Ubl conjugation pathway SUBCELLULAR LOCATION: Cytoplasm Q8N2F6,PROTEIN NAMES: Armadillo repeat-containing protein 10 (Splicing variant involved in hepatocarcinogenesis protein) ORGANISM: Homo sapiens (Human) FUNCTION: May play a role in cell survival and cell growth. May suppress the transcriptional activity of p53/TP53. KEYWORDS: Alternative splicing;Direct protein sequencing;Endoplasmic reticulum;Growth regulation;Membrane;Mitochondrion;Mitochondrion outer membrane;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Single-pass membrane protein.; SUBCELLULAR LOCATION: [Isoform 2]: Mitochondrion outer membrane ; Single-pass membrane protein MISCELLANEOUS: Depletion of isoform 2 results in cell apoptosis while its overexpression in cells leads to accelerated growth rate and tumorogenicity. Q8N2H9,"PROTEIN NAMES: E3 ubiquitin-protein ligase pellino homolog 3 (Pellino-3) PROTEIN FAMILY: Pellino family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the pellino family. FUNCTION: E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. Involved in the TLR and IL-1 signaling pathways via interaction with the complex containing IRAK kinases and TRAF6. Mediates 'Lys-63'-linked polyubiquitination of IRAK1. Can activate AP1/JUN and ELK1. Acts as a regulator of innate immunity by mediating 'Lys-63'-linked polyubiquitination of RIPK2 downstream of NOD1 and NOD2, thereby transforming RIPK2 into a scaffolding protein for downstream effectors, ultimately leading to activation of the NF-kappa-B and MAP kinases signaling (By similarity). Catalyzes 'Lys-63'-linked polyubiquitination of RIPK2 in parallel of XIAP (By similarity). PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Alternative splicing;Immunity;Innate immunity;Phosphoprotein;Reference proteome;Transferase;Ubl conjugation pathway " Q8N2R0,PROTEIN NAMES: Protein odd-skipped-related 2 PROTEIN FAMILY: Odd C2H2-type zinc-finger protein family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the Odd C2H2-type zinc-finger protein family. FUNCTION: May be involved in the development of the mandibular molar tooth germ at the bud stage. KEYWORDS: Alternative splicing;Metal-binding;Nucleus;Reference proteome;Repeat;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Q8N393,PROTEIN NAMES: Zinc finger protein 786 PROTEIN FAMILY: Krueppel C2H2-type zinc-finger protein family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein family. FUNCTION: May be involved in transcriptional regulation. KEYWORDS: Alternative splicing;DNA-binding;Metal-binding;Nucleus;Reference proteome;Repeat;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Q8N3G9,PROTEIN NAMES: Transmembrane protein 130 ORGANISM: Homo sapiens (Human) KEYWORDS: Alternative splicing;Glycoprotein;Golgi apparatus;Membrane;Reference proteome;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus membrane ; Single-pass type I membrane protein Q8N3T1,"PROTEIN NAMES: Polypeptide N-acetylgalactosaminyltransferase 15 (Polypeptide GalNAc transferase-like protein 2) (GalNAc-T-like protein 2) (pp-GaNTase-like protein 2) (Polypeptide N-acetylgalactosaminyltransferase-like protein 2) (Protein-UDP acetylgalactosaminyltransferase-like protein 2) (UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase-like protein 2) PROTEIN FAMILY: Glycosyltransferase 2 family, GalNAc-T subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily. FUNCTION: Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Although it displays a much weaker activity toward all substrates tested compared to GALNT2, it is able to transfer up to seven GalNAc residues to the Muc5AC peptide, suggesting that it can fill vicinal Thr/Ser residues in cooperation with other GALNT proteins. Prefers Muc1a as substrate. PATHWAY: Protein modification; protein glycosylation. KEYWORDS: Disulfide bond;Glycoprotein;Glycosyltransferase;Golgi apparatus;Lectin;Manganese;Membrane;Metal-binding;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus membrane ; Single-pass type II membrane protein " Q8N4Y2,PROTEIN NAMES: EF-hand calcium-binding domain-containing protein 4A (Calcium release-activated calcium channel regulator 2B) (CRAC channel regulator 2B) (Calcium release-activated channel regulator 2B) PROTEIN FAMILY: EFCAB4 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the EFCAB4 family. FUNCTION: Plays a role in store-operated Ca(2+) entry (SOCE). KEYWORDS: Alternative splicing;Coiled coil;Phosphoprotein;Reference proteome;Repeat Q8N5A5,"PROTEIN NAMES: Zinc finger CCCH-type with G patch domain-containing protein (G patch domain-containing protein 6) (Zinc finger CCCH domain-containing protein 9) (Zinc finger and G patch domain-containing protein) ORGANISM: Homo sapiens (Human) FUNCTION: Transcription repressor that specifically binds the 5'-GGAG[GA]A[GA]A-3' consensus sequence. Represses transcription by recruiting the chromatin multiprotein complex NuRD to target promoters. Negatively regulates expression of EGFR, a gene involved in cell proliferation, survival and migration. Its ability to repress genes of the EGFR pathway suggest it may act as a tumor suppressor. Able to suppress breast carcinogenesis.; FUNCTION: [Isoform 4]: Antagonizes the transcription repression by isoform 1 by competing for the binding of the NuRD complex. Does not bind DNA. KEYWORDS: 3D-structure;Acetylation;Alternative splicing;DNA-binding;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Repressor;Transcription;Transcription regulation;Ubl conjugation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus.; SUBCELLULAR LOCATION: [Isoform 4]: Nucleus " Q8N5Z5,"PROTEIN NAMES: BTB/POZ domain-containing protein KCTD17 ORGANISM: Homo sapiens (Human) FUNCTION: Substrate-adapter for CUL3-RING ubiquitin ligase complexes which mediates the ubiquitination and subsequent proteasomal degradation of TCHP, a protein involved in ciliogenesis down-regulation. Thereby, positively regulates ciliogenesis, playing a crucial role in the initial steps of axoneme extension. May also play a role in endoplasmic reticulum calcium ion homeostasis. KEYWORDS: 3D-structure;Alternative splicing;Cilium biogenesis/degradation;Coiled coil;Cytoplasm;Disease variant;Dystonia;Reference proteome;Ubl conjugation pathway SUBCELLULAR LOCATION: Cytoplasm " Q8N7C3,PROTEIN NAMES: Probable E3 ubiquitin-protein ligase TRIML2 (RING-type E3 ubiquitin transferase TRIML2) (SPRY domain-containing protein 6) (Tripartite motif family-like protein 2) ORGANISM: Homo sapiens (Human) PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Alternative splicing;Coiled coil;Metal-binding;Reference proteome;Transferase;Ubl conjugation pathway;Zinc;Zinc-finger Q8N7W2,PROTEIN NAMES: BEN domain-containing protein 7 ORGANISM: Homo sapiens (Human) KEYWORDS: Alternative splicing;Isopeptide bond;Phosphoprotein;Reference proteome;Ubl conjugation Q8N884,"PROTEIN NAMES: Cyclic GMP-AMP synthase (cGAMP synthase) (cGAS) (h-cGAS) (2'3'-cGAMP synthase) (Mab-21 domain-containing protein 1) PROTEIN FAMILY: Mab-21 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the mab-21 family. FUNCTION: Nucleotidyltransferase that catalyzes the formation of cyclic GMP-AMP (2',3'-cGAMP) from ATP and GTP and plays a key role in innate immunity. Catalysis involves both the formation of a 2',5' phosphodiester linkage at the GpA step and the formation of a 3',5' phosphodiester linkage at the ApG step, producing c[G(2',5')pA(3',5')p]. Acts as a key DNA sensor: directly binds double-stranded DNA (dsDNA), inducing the formation of liquid-like droplets in which CGAS is activated, leading to synthesis of 2',3'-cGAMP, a second messenger that binds to and activates STING1, thereby triggering type-I interferon production. Preferentially recognizes and binds curved long dsDNAs of a minimal length of 40 bp. Acts as a key foreign DNA sensor, the presence of double-stranded DNA (dsDNA) in the cytoplasm being a danger signal that triggers the immune responses. Has antiviral activity by sensing the presence of dsDNA from DNA viruses in the cytoplasm. Also acts as an innate immune sensor of infection by retroviruses, such as HIV-2, by detecting the presence of reverse-transcribed DNA in the cytosol. In contrast, HIV-1 is poorly sensed by CGAS, due to its capsid that cloaks viral DNA from CGAS detection. Detection of retroviral reverse-transcribed DNA in the cytosol may be indirect and be mediated via interaction with PQBP1, which directly binds reverse-transcribed retroviral DNA. Also detects the presence of DNA from bacteria, such as M.tuberculosis. 2',3'-cGAMP can be transferred from producing cells to neighboring cells through gap junctions, leading to promote STING1 activation and convey immune response to connecting cells. 2',3'-cGAMP can also be transferred between cells by virtue of packaging within viral particles contributing to IFN-induction in newly infected cells in a cGAS-independent but STING1-dependent manner. Also senses the presence of neutrophil extracellular traps (NETs) that are translocated to the cytosol following phagocytosis, leading to synthesis of 2',3'-cGAMP. In addition to foreign DNA, can also be activated by endogenous nuclear or mitochondrial DNA. When self-DNA leaks into the cytosol during cellular stress (such as mitochondrial stress, SARS-CoV-2 infection causing severe COVID-19 disease, DNA damage, mitotic arrest or senescence), or is present in form of cytosolic micronuclei, CGAS is activated leading to a state of sterile inflammation. Acts as a regulator of cellular senescence by binding to cytosolic chromatin fragments that are present in senescent cells, leading to trigger type-I interferon production via STING1 and promote cellular senescence (By similarity). Also involved in the inflammatory response to genome instability and double-stranded DNA breaks: acts by localizing to micronuclei arising from genome instability. Micronuclei, which are frequently found in cancer cells, consist of chromatin surrounded by their own nuclear membrane: following breakdown of the micronuclear envelope, a process associated with chromothripsis, CGAS binds self-DNA exposed to the cytosol, leading to 2',3'-cGAMP synthesis and subsequent activation of STING1 and type-I interferon production. Activated in response to prolonged mitotic arrest, promoting mitotic cell death. In a healthy cell, CGAS is however kept inactive even in cellular events that directly expose it to self-DNA, such as mitosis, when cGAS associates with chromatin directly after nuclear envelope breakdown or remains in the form of postmitotic persistent nuclear cGAS pools bound to chromatin. Nuclear CGAS is inactivated by chromatin via direct interaction with nucleosomes, which block CGAS from DNA binding and thus prevent CGAS-induced autoimmunity. Also acts as a suppressor of DNA repair in response to DNA damage: inhibits homologous recombination repair by interacting with PARP1, the CGAS-PARP1 interaction leading to impede the formation of the PARP1-TIMELESS complex. In addition to DNA, also sense translation stress: in response to translation stress, translocates to the cytosol and associates with collided ribosomes, promoting its activation and triggering type-I interferon production. In contrast to other mammals, human CGAS displays species-specific mechanisms of DNA recognition and produces less 2',3'-cGAMP, allowing a more fine-tuned response to pathogens. KEYWORDS: 3D-structure;Acetylation;ADP-ribosylation;Alternative splicing;Antiviral defense;ATP-binding;Cell membrane;Chromosome;Cytoplasm;DNA damage;DNA repair;DNA-binding;GTP-binding;Host-virus interaction;Immunity;Innate immunity;Isopeptide bond;Lipid-binding;Lipoprotein;Magnesium;Membrane;Metal-binding;Methylation;Nucleotide-binding;Nucleotidyltransferase;Nucleus;Palmitate;Phosphoprotein;Reference proteome;Transferase;Ubl conjugation;Zinc SUBCELLULAR LOCATION: Nucleus Chromosome Cell membrane ; Peripheral membrane protein Cytoplasm, cytosol Note=Mainly localizes in the nucleus, and at low level in the cytosol. On chromosomes, enriched on centromeric satellite and LINE DNA repeat elements. Exported from the nucleus to the cytosol in a XPO1/CRM1 via the nuclear export signal in response to DNA stimulation. Outside the nucleus, localizes at the cell membrane as a peripheral membrane protein in resting conditions: association to the cell membrane is mediated via binding to phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). Localization at the cell membrane is required to limit the recognition of self-DNA. Following detection of double-stranded DNA (dsDNA), released from the cell membrane into the cytosol in order to signal. Upon transfection with dsDNA forms punctate structures that co-localize with DNA and Beclin-1 (BECN1). Phosphorylation at Tyr-215 promotes cytosolic retention. In response to translation stress, translocates to the cytosol and associates with collided ribosomes.; SUBCELLULAR LOCATION: Note=(Microbial infection) Upon infection with virulent M.tuberculosis forms aggregates with dsDNA, non-virulent bacteria (without the ESX-1 locus) do not form these aggregates. MISCELLANEOUS: The cGAS-STING signaling pathway drives sterile inflammation leading to type I interferon immunopathology in severe COVID-19 disease caused by SARS-CoV-2 virus infection. Tissue damages in the lung and skin lesions are caused by activation of the cGAS-STING signaling leading to aberrant inflammation. Endothelial cell damage is also caused by activation of the cGAS-STING pathway: SARS-CoV-2 infection triggers mitochondrial DNA release into the cytosol. Released mitochondrial DNA is then detected by CGAS, leading to activation of the cGAS-STING pathway, triggering type-I interferon production and autoinflammation." Q8N9B5,"PROTEIN NAMES: Junction-mediating and -regulatory protein PROTEIN FAMILY: JMY family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the JMY family. FUNCTION: Acts both as a nuclear p53/TP53-cofactor and a cytoplasmic regulator of actin dynamics depending on conditions. In nucleus, acts as a cofactor that increases p53/TP53 response via its interaction with p300/EP300. Increases p53/TP53-dependent transcription and apoptosis, suggesting an important role in p53/TP53 stress response such as DNA damage. In cytoplasm, acts as a nucleation-promoting factor for both branched and unbranched actin filaments. Activates the Arp2/3 complex to induce branched actin filament networks. Also catalyzes actin polymerization in the absence of Arp2/3, creating unbranched filaments. Contributes to cell motility by controlling actin dynamics. May promote the rapid formation of a branched actin network by first nucleating new mother filaments and then activating Arp2/3 to branch off these filaments. Upon nutrient stress, directly recruited by MAP1LC3B to the phagophore membrane surfaces to promote actin assembly during autophagy. The p53/TP53-cofactor and actin activator activities are regulated via its subcellular location (By similarity). KEYWORDS: Actin-binding;Alternative splicing;Coiled coil;Cytoplasm;Cytoplasmic vesicle;Cytoskeleton;DNA damage;DNA repair;Lipoprotein;Membrane;Nucleus;Phosphoprotein;Reference proteome;Ubl conjugation SUBCELLULAR LOCATION: Nucleus Cytoplasmic vesicle Cytoplasm, cytoskeleton Endomembrane system; Lipid-anchor. Cytoplasmic vesicle, autophagosome membrane Note=Localizes to the nucleus in most cell types. Accumulates in nucleus under DNA damage conditions, increasing p53/TP53 transcription response and reducing its influence on cell motility (By similarity). In primary neutrophils, it colocalizes with actin filaments at the leading edge and is excluded from the nucleus. Localization correlates with motility, because it moves from the nucleus to the cytoplasmic compartment when cells are differentiated from nonmotile cells into highly motile neutrophil-like cells. Localizes to cytoplasmic vesicles which associate with actin filament and autophagosomal membranes upon starvation-induced autophagy. " Q8N9F0,PROTEIN NAMES: N-acetylaspartate synthetase (NAA synthetase) (Camello-like protein 3) (N-acetyltransferase 8-like protein) PROTEIN FAMILY: Camello family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the camello family. FUNCTION: Catalyzes the synthesis of N-acetylaspartate acid (NAA) from L-aspartate and acetyl-CoA. Promotes dopamine uptake by regulating TNF-alpha expression (By similarity). Attenuates methamphetamine-induced inhibition of dopamine uptake. KEYWORDS: Acyltransferase;Cytoplasm;Endoplasmic reticulum;Membrane;Microsome;Mitochondrion;Reference proteome;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cytoplasm Microsome membrane ; Single-pass membrane protein Mitochondrion membrane ; Single-pass membrane protein Endoplasmic reticulum membrane ; Single-pass membrane protein Note=Its enzymatic activity contribution is quantitatively larger in mitochondrial compartment than in extramitochondrial compartment. Q8N9N2,"PROTEIN NAMES: Activating signal cointegrator 1 complex subunit 1 (ASC-1 complex subunit p50) (Trip4 complex subunit p50) ORGANISM: Homo sapiens (Human) FUNCTION: Plays a role in DNA damage repair as component of the ASCC complex. Part of the ASC-1 complex that enhances NF-kappa-B, SRF and AP1 transactivation. In cells responding to gastrin-activated paracrine signals, it is involved in the induction of SERPINB2 expression by gastrin. May also play a role in the development of neuromuscular junction. KEYWORDS: Alternative splicing;Direct protein sequencing;DNA damage;DNA repair;Neurodegeneration;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus Nucleus speckle Note=Colocalizes with PRPF8 in nuclear speckles in the absence of DNA damage. " Q8NA54,"PROTEIN NAMES: IQ motif and ubiquitin-like domain-containing protein ORGANISM: Homo sapiens (Human) FUNCTION: Adapter protein that anchors the radial spoke 1 (RS1) complex to the A microtubule of outer doublet microtubules in axonemes. The triple radial spokes (RS1, RS2 and RS3) are required to modulate beating of the sperm flagellum. May play a role in inhibiting signaling via MAPK1/ERK2 and MAPK3/ERK1. Additionally, may play a role in the functioning of cilia (By similarity). Not required for the functioning of tracheal or ependymal cilia (By similarity). KEYWORDS: 3D-structure;Alternative splicing;Cell projection;Cilium;Cilium biogenesis/degradation;Cytoplasm;Cytoskeleton;Disease variant;Flagellum;Reference proteome SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, flagellum axoneme Cell projection, cilium Note=Localizes to the axoneme of sperm cells and the cilia of tracheal epithelial cells. " Q8NA77,"PROTEIN NAMES: Testis-expressed protein 19 ORGANISM: Homo sapiens (Human) FUNCTION: Required during spermatogenesis and placenta development, participating in the repression of retrotransposable elements and prevent their mobilization. Collaborates with the Piwi-interacting RNA (piRNA) pathway, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins. Interacts with Piwi proteins and directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Also during spermatogenesis, promotes, with UBR2, SPO11-dependent recombination foci to accumulate and drive robust homologous chromosome synapsis (By similarity). Interacts with LINE-1 retrotransposon encoded LIRE1, stimulates LIRE1 polyubiquitination, mediated by UBR2, and degradation, inhibiting LINE-1 retrotransposon mobilization. KEYWORDS: Cytoplasm;Differentiation;Meiosis;Reference proteome;Spermatogenesis SUBCELLULAR LOCATION: Cytoplasm Note=Was initially reported to localize in the nucleus. However, it was later shown to localize in cytoplasm only. Cytoplasmic localization is distinct from the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon activity during meiosis. " Q8NAP8,"PROTEIN NAMES: Zinc finger and BTB domain-containing protein 8B ORGANISM: Homo sapiens (Human) FUNCTION: May be involved in transcriptional regulation. KEYWORDS: Alternative splicing;DNA-binding;Metal-binding;Nucleus;Reference proteome;Repeat;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: [Isoform 2]: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay." Q8NBB4,PROTEIN NAMES: Zinc finger and SCAN domain-containing protein 1 ORGANISM: Homo sapiens (Human) FUNCTION: May be involved in transcriptional regulation. KEYWORDS: Alternative splicing;DNA-binding;Metal-binding;Nucleus;Reference proteome;Repeat;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Q8NBV8,"PROTEIN NAMES: Synaptotagmin-8 (Synaptotagmin VIII) (SytVIII) PROTEIN FAMILY: Synaptotagmin family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the synaptotagmin family. FUNCTION: Involved in the trafficking and exocytosis of secretory vesicles in non-neuronal tissues. Mediates Ca(2+)-regulation of exocytosis acrosomal reaction in sperm. May mediate Ca(2+)-regulation of exocytosis in insulin secreted cells. KEYWORDS: Alternative splicing;Cell membrane;Cytoplasmic vesicle;Membrane;Reference proteome;Repeat;Signal-anchor;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type III membrane protein Cytoplasmic vesicle, secretory vesicle, acrosome " Q8NC42,"PROTEIN NAMES: E3 ubiquitin-protein ligase RNF149 (DNA polymerase-transactivated protein 2) (RING finger protein 149) (RING-type E3 ubiquitin transferase RNF149) ORGANISM: Homo sapiens (Human) FUNCTION: E3 ubiquitin-protein ligase. Ubiquitinates BRAF, inducing its proteasomal degradation. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Glycoprotein;Membrane;Metal-binding;Phosphoprotein;Reference proteome;Signal;Transferase;Transmembrane;Transmembrane helix;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Membrane ; Single-pass membrane protein " Q8NC44,"PROTEIN NAMES: Reticulophagy regulator 2 PROTEIN FAMILY: RETREG family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the RETREG family. FUNCTION: Endoplasmic reticulum (ER)-anchored autophagy regulator which exists in an inactive state under basal conditions but is activated following cellular stress. When activated, induces ER fragmentation and mediates ER delivery into lysosomes through sequestration into autophagosomes via interaction with ATG8 family proteins. Required for collagen quality control in a LIR motif-independent manner (By similarity). KEYWORDS: 3D-structure;Autophagy;Endoplasmic reticulum;Membrane;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein " Q8NCK7,"PROTEIN NAMES: Monocarboxylate transporter 11 (MCT 11) (Solute carrier family 16 member 11) PROTEIN FAMILY: Major facilitator superfamily, Monocarboxylate porter family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family. FUNCTION: Proton-linked monocarboxylate transporter. It catalyzes the transport of pyruvate across the plasma membrane. Probably involved in hepatic lipid metabolism: overexpression results in an increase of triacylglycerol(TAG) levels, small increases in intracellular diacylglycerols and decreases in lysophosphatidylcholine, cholesterol ester and sphingomyelin lipids. KEYWORDS: Cell membrane;Diabetes mellitus;Endoplasmic reticulum;Lipid metabolism;Membrane;Reference proteome;Symport;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein Cell membrane ; Multi-pass membrane protein " Q8NCP5,PROTEIN NAMES: Zinc finger and BTB domain-containing protein 44 (BTB/POZ domain-containing protein 15) (Zinc finger protein 851) ORGANISM: Homo sapiens (Human) FUNCTION: May be involved in transcriptional regulation. KEYWORDS: Alternative splicing;DNA-binding;Isopeptide bond;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Repeat;Transcription;Transcription regulation;Ubl conjugation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Q8NCS7,PROTEIN NAMES: Choline transporter-like protein 5 (Solute carrier family 44 member 5) PROTEIN FAMILY: CTL (choline transporter-like) family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the CTL (choline transporter-like) family. FUNCTION: Choline/H+ antiporter. KEYWORDS: Alternative splicing;Antiport;Cell membrane;Glycoprotein;Membrane;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Q8NDH6,PROTEIN NAMES: Islet cell autoantigen 1-like protein (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 14 protein) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 15 protein) ORGANISM: Homo sapiens (Human) KEYWORDS: Alternative splicing;Reference proteome Q8NEC5,"PROTEIN NAMES: Cation channel sperm-associated protein 1 (CatSper1) (hCatSper) PROTEIN FAMILY: Cation channel sperm-associated family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the cation channel sperm-associated (TC 1.A.1.19) family. FUNCTION: Pore-forming subunit of the CatSper complex, a sperm-specific voltage-gated calcium channel that plays a central role in calcium-dependent physiological responses essential for successful fertilization, such as sperm hyperactivation, acrosome reaction and chemotaxis towards the oocyte. KEYWORDS: Calcium;Calcium channel;Calcium transport;Cell membrane;Cell projection;Cilium;Developmental protein;Differentiation;Flagellum;Ion channel;Ion transport;Membrane;Reference proteome;Spermatogenesis;Transmembrane;Transmembrane helix;Transport;Voltage-gated channel SUBCELLULAR LOCATION: Cell projection, cilium, flagellum membrane ; Multi-pass membrane protein Note=Specifically located in the principal piece of the sperm tail. " Q8NET6,"PROTEIN NAMES: Carbohydrate sulfotransferase 13 (Chondroitin 4-O-sulfotransferase 3) (Chondroitin 4-sulfotransferase 3) (C4ST-3) (C4ST3) PROTEIN FAMILY: Sulfotransferase 2 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the sulfotransferase 2 family. FUNCTION: Catalyzes the transfer of sulfate to position 4 of the N-acetylgalactosamine (GalNAc) residue of chondroitin. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage and is distributed on the surfaces of many cells and extracellular matrices. Transfers sulfate to the C4 hydroxyl of beta1,4-linked GalNAc that is substituted with a beta-linked glucuronic acid at the C-3 hydroxyl. No activity toward dermatan. KEYWORDS: Alternative splicing;Carbohydrate metabolism;Glycoprotein;Golgi apparatus;Membrane;Phosphoprotein;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus membrane ; Single-pass type II membrane protein " Q8NFF5,"PROTEIN NAMES: FAD synthase (FAD pyrophosphorylase) (FMN adenylyltransferase) (Flavin adenine dinucleotide synthase) [Includes: Molybdenum cofactor biosynthesis protein-like region; FAD synthase region] PROTEIN FAMILY: MoaB/Mog family; PAPS reductase family, FAD1 subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: In the N-terminal section; belongs to the MoaB/Mog family.; SIMILARITY: In the C-terminal section; belongs to the PAPS reductase family. FAD1 subfamily. FUNCTION: Catalyzes the adenylation of flavin mononucleotide (FMN) to form flavin adenine dinucleotide (FAD) coenzyme. PATHWAY: Cofactor biosynthesis; FAD biosynthesis; FAD from FMN: step 1/1. KEYWORDS: Acetylation;Alternative splicing;ATP-binding;Cytoplasm;Disease variant;FAD;Flavoprotein;FMN;Mitochondrion;Nucleotide-binding;Nucleotidyltransferase;Phosphoprotein;Primary mitochondrial disease;Reference proteome;Transferase;Transit peptide SUBCELLULAR LOCATION: [Isoform 1]: Mitochondrion matrix.; SUBCELLULAR LOCATION: [Isoform 2]: Cytoplasm. " Q8NFH4,"PROTEIN NAMES: Nucleoporin Nup37 (p37) (Nup107-160 subcomplex subunit Nup37) ORGANISM: Homo sapiens (Human) FUNCTION: Component of the Nup107-160 subcomplex of the nuclear pore complex (NPC). The Nup107-160 subcomplex is required for the assembly of a functional NPC. The Nup107-160 subcomplex is also required for normal kinetochore microtubule attachment, mitotic progression and chromosome segregation. KEYWORDS: 3D-structure;Cell cycle;Cell division;Centromere;Chromosome;Chromosome partition;Disease variant;Kinetochore;Mitosis;mRNA transport;Nuclear pore complex;Nucleus;Primary microcephaly;Protein transport;Reference proteome;Repeat;Translocation;Transport;WD repeat SUBCELLULAR LOCATION: Chromosome, centromere, kinetochore. Nucleus, nuclear pore complex. " Q8NFT2,"PROTEIN NAMES: Metalloreductase STEAP2 (Prostate cancer-associated protein 1) (Protein up-regulated in metastatic prostate cancer) (PUMPCn) (Six-transmembrane epithelial antigen of prostate 2) (SixTransMembrane protein of prostate 1) PROTEIN FAMILY: STEAP family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the STEAP family. FUNCTION: Integral membrane protein that functions as a NADPH-dependent ferric-chelate reductase, using NADPH from one side of the membrane to reduce a Fe(3+) chelate that is bound on the other side of the membrane (By similarity). Mediates sequential transmembrane electron transfer from NADPH to FAD and onto heme, and finally to the Fe(3+) chelate (By similarity). Can also reduce Cu(2+) to Cu(1+) (By similarity). KEYWORDS: 3D-structure;Alternative splicing;Cell membrane;Copper;Electron transport;Endosome;FAD;Flavoprotein;Heme;Ion transport;Iron;Iron transport;Membrane;Metal-binding;NAD;NADP;Oxidoreductase;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Endosome membrane ; Multi-pass membrane protein Cell membrane ; Multi-pass membrane protein " Q8NFU1,"PROTEIN NAMES: Bestrophin-2a (Vitelliform macular dystrophy 2-like protein 1) PROTEIN FAMILY: Anion channel-forming bestrophin family, Calcium-sensitive chloride channel subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the anion channel-forming bestrophin (TC 1.A.46) family. Calcium-sensitive chloride channel subfamily. FUNCTION: Ligand-gated anion channel that allows the movement of anions across cell membranes when activated by calcium (Ca2+). Transports a large specter of anions, namely mediates the movement of chloride, L-glutamate and iodide. Calcium-binding triggers the dilation of the aperture, but calcium-dependent gating is only effective when the size of the passing anion is bigger than the closed aperture (By similarity). Mediates the calcium-activated hydrogencarbonate movement and participates in colonic hydrogencarbonate secretion concomitant with mucin secretion (By similarity). In non-pigmented epithelium (NPE), mediates the efflux of intracellular L-glutamate; binding of intracellular L-glutamate activates and open both the neck and the aperture of the channel, leading to L-glutamate exit promoting chloride influx movement from the extracellular side in trans. Also exhibits a directional permeability for intracellular glutamine, in a similar manner as for L-glutamate. KEYWORDS: 3D-structure;Calcium;Cell membrane;Chloride;Chloride channel;Ion channel;Ion transport;Membrane;Metal-binding;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Basolateral cell membrane ; Multi-pass membrane protein. " Q8NG06,"PROTEIN NAMES: E3 ubiquitin-protein ligase TRIM58 (Protein BIA2) (RING-type E3 ubiquitin transferase TRIM58) (Tripartite motif-containing protein 58) PROTEIN FAMILY: TRIM/RBCC family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the TRIM/RBCC family. FUNCTION: E3 ubiquitin ligase induced during late erythropoiesis. Directly binds and ubiquitinates the intermediate chain of the microtubule motor dynein (DYNC1LI1/DYNC1LI2), stimulating the degradation of the dynein holoprotein complex. May participate in the erythroblast enucleation process through regulation of nuclear polarization. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: 3D-structure;Coiled coil;Metal-binding;Reference proteome;Transferase;Ubl conjugation pathway;Zinc;Zinc-finger " Q8NG27,PROTEIN NAMES: E3 ubiquitin-protein ligase Praja-1 (Praja1) (RING finger protein 70) (RING-type E3 ubiquitin transferase Praja-1) ORGANISM: Homo sapiens (Human) FUNCTION: Has E2-dependent E3 ubiquitin-protein ligase activity. Ubiquitinates MAGED1 antigen leading to its subsequent degradation by proteasome (By similarity). May be involved in protein sorting. KEYWORDS: 3D-structure;Alternative splicing;Metal-binding;Phosphoprotein;Reference proteome;Transferase;Ubl conjugation;Ubl conjugation pathway;Zinc;Zinc-finger MISCELLANEOUS: [Isoform 2]: previous research reported that isoform 2 arises by alternative initiation. Q8NG66,"PROTEIN NAMES: Serine/threonine-protein kinase Nek11 (Never in mitosis A-related kinase 11) (NimA-related protein kinase 11) PROTEIN FAMILY: Protein kinase superfamily, NEK Ser/Thr protein kinase family, NIMA subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily. FUNCTION: Protein kinase which plays an important role in the G2/M checkpoint response to DNA damage. Controls degradation of CDC25A by directly phosphorylating it on residues whose phosphorylation is required for BTRC-mediated polyubiquitination and degradation. KEYWORDS: Alternative splicing;ATP-binding;Cell cycle;Coiled coil;Kinase;Magnesium;Manganese;Metal-binding;Nucleotide-binding;Nucleus;Phosphoprotein;Reference proteome;Serine/threonine-protein kinase;Transferase SUBCELLULAR LOCATION: Nucleus Nucleus, nucleolus Note=Nuclear during interphase but moves to the polar microtubules during prometaphase and metaphase. Accumulates in the nucleolus in G1/S-arrested cells. " Q8NHG8,"PROTEIN NAMES: E3 ubiquitin-protein ligase ZNRF2 (Protein Ells2) (RING finger protein 202) (RING-type E3 ubiquitin transferase ZNRF2) (Zinc/RING finger protein 2) ORGANISM: Homo sapiens (Human) FUNCTION: E3 ubiquitin-protein ligase that plays a role in the establishment and maintenance of neuronal transmission and plasticity. Ubiquitinates the Na(+)/K(+) ATPase alpha-1 subunit/ATP1A1 and thereby influences its endocytosis and/or degradation. Acts also as a positive regulator of mTORC1 activation by amino acids, which functions upstream of the V-ATPase and of Rag-GTPases. In turn, phosphorylation by mTOR leads to its inhibition via targeting to the cytosol allowing a self-regulating feedback mechanism. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Cell membrane;Cell projection;Cytoplasm;Endosome;Lipoprotein;Lysosome;Membrane;Metal-binding;Myristate;Phosphoprotein;Reference proteome;Synapse;Transferase;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Endosome membrane ; Peripheral membrane protein Lysosome membrane ; Peripheral membrane protein Presynaptic cell membrane ; Peripheral membrane protein Cytoplasm " Q8NHH1,"PROTEIN NAMES: Tubulin polyglutamylase TTLL11 (Tubulin--tyrosine ligase-like protein 11) PROTEIN FAMILY: Tubulin--tyrosine ligase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the tubulin--tyrosine ligase family. FUNCTION: Polyglutamylase which modifies tubulin, generating polyglutamate side chains of variable lengths on the gamma-carboxyl group of specific glutamate residues within the C-terminal tail of tubulin. Preferentially mediates ATP-dependent polyglutamate long side-chain elongation over the initiation step of the polyglutamylation reaction. Preferentially modifies the alpha-tubulin tail over a beta-tail (By similarity). Required for CCSAP localization to both spindle and cilia microtubules. Promotes tubulin polyglutamylation which stimulates spastin/SPAST-mediated microtubule severing, thereby regulating microtubule functions (By similarity). KEYWORDS: Alternative splicing;ATP-binding;Cell projection;Cilium;Cytoplasm;Cytoskeleton;Ligase;Magnesium;Metal-binding;Microtubule;Nucleotide-binding;Reference proteome SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, cilium basal body Cytoplasm, cytoskeleton " Q8NHV1,"PROTEIN NAMES: GTPase IMAP family member 7 (Immunity-associated nucleotide 7 protein) (IAN-7) PROTEIN FAMILY: TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily, AIG1/Toc34/Toc159-like paraseptin GTPase family, IAN subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. AIG1/Toc34/Toc159-like paraseptin GTPase family. IAN subfamily. FUNCTION: The dimer has GTPase activity; the active site contains residues from both subunits. KEYWORDS: 3D-structure;Cytoplasm;Endoplasmic reticulum;Golgi apparatus;GTP-binding;Lipid droplet;Nucleotide-binding;Reference proteome SUBCELLULAR LOCATION: Lipid droplet Cytoplasm Endoplasmic reticulum Golgi apparatus Note=Colocalizes with GIMAP2 on the surface of cytoplasmic lipid droplets. " Q8NHY0,"PROTEIN NAMES: Beta-1,4 N-acetylgalactosaminyltransferase 2 (Sd(a) beta-1,4-GalNAc transferase) (UDP-GalNAc:Neu5Aca2-3Galb-R b1,4-N-acetylgalactosaminyltransferase) PROTEIN FAMILY: Glycosyltransferase 2 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the glycosyltransferase 2 family. FUNCTION: Involved in the synthesis of the Sd(a) antigen (Sia-alpha2,3-[GalNAc-beta1,4]Gal-beta1,4-GlcNAc), a carbohydrate determinant expressed on erythrocytes, the colonic mucosa and other tissues. Transfers a beta-1,4-linked GalNAc to the galactose residue of an alpha-2,3-sialylated chain. PATHWAY: Protein modification; protein glycosylation. KEYWORDS: Alternative splicing;Glycosyltransferase;Golgi apparatus;Membrane;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus membrane ; Single-pass type II membrane protein " Q8NI51,"PROTEIN NAMES: Transcriptional repressor CTCFL (Brother of the regulator of imprinted sites) (CCCTC-binding factor) (CTCF paralog) (CTCF-like protein) (Cancer/testis antigen 27) (CT27) (Zinc finger protein CTCF-T) PROTEIN FAMILY: CTCF zinc-finger protein family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the CTCF zinc-finger protein family. FUNCTION: Testis-specific DNA binding protein responsible for insulator function, nuclear architecture and transcriptional control, which probably acts by recruiting epigenetic chromatin modifiers. Plays a key role in gene imprinting in male germline, by participating in the establishment of differential methylation at the IGF2/H19 imprinted control region (ICR). Directly binds the unmethylated H19 ICR and recruits the PRMT7 methyltransferase, leading to methylate histone H4 'Arg-3' to form H4R3sme2. This probably leads to recruit de novo DNA methyltransferases at these sites (By similarity). Seems to act as tumor suppressor. In association with DNMT1 and DNMT3B, involved in activation of BAG1 gene expression by binding to its promoter. Required for dimethylation of H3 lysine 4 (H3K4me2) of MYC and BRCA1 promoters. KEYWORDS: Activator;Alternative splicing;Cell cycle;Chromatin regulator;Cytoplasm;DNA-binding;Metal-binding;Nucleus;Reference proteome;Repeat;Repressor;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cytoplasm. Nucleus. " Q8NK92,"PROTEIN NAMES: Aorsin ORGANISM: Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) FUNCTION: Serine endopeptidase which hydrolyzes a range of fluorogenic peptide substrates containing the basic residues arginine or lysine at the P1 position and prefers paired basic resides. Also hydrolyzes clupeine and salmine, activates plasminogen and converts trypsinogen to trypsin. KEYWORDS: Calcium;Direct protein sequencing;Glycoprotein;Hydrolase;Metal-binding;Protease;Reference proteome;Secreted;Serine protease;Signal;Zymogen SUBCELLULAR LOCATION: Secreted, extracellular space " Q8NNJ2,"PROTEIN NAMES: Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex (Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex) (Pyruvate dehydrogenase complex component E2) (PDH component E2) PROTEIN FAMILY: 2-oxoacid dehydrogenase family ORGANISM: Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025) SIMILARITY: Belongs to the 2-oxoacid dehydrogenase family. FUNCTION: Is essential for both 2-oxoglutarate dehydrogenase (ODH) and pyruvate dehydrogenase (PDH) activities, but AceF has exclusively transacetylase (and no transsuccinylase) activity. The lipoyl residues required for ODH activity are likely provided by AceF. KEYWORDS: 3D-structure;Acyltransferase;Lipoyl;Reference proteome;Repeat;Transferase MISCELLANEOUS: Is the only lipoylated protein in C.glutamicum." Q8PDQ6,PROTEIN NAMES: FAD-dependent urate hydroxylase (Flavoprotein urate hydroxylase) PROTEIN FAMILY: HpyO family ORGANISM: Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25) SIMILARITY: Belongs to the HpyO family. FUNCTION: Catalyzes the hydroxylation of urate to 5-hydroxyisourate (HIU). Is likely to be involved in the urate degradation pathway to allantoin. Is slightly more efficient (about 2.6 times) with NADPH than NADH as the electron donor. PATHWAY: Purine metabolism; urate degradation. KEYWORDS: FAD;Flavoprotein;Monooxygenase;NAD;NADP;Oxidoreductase;Purine metabolism;Reference proteome Q8PDX2,"PROTEIN NAMES: Acyl-CoA:acyl-CoA alkyltransferase PROTEIN FAMILY: Thiolase-like superfamily, OleA family ORGANISM: Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25) SIMILARITY: Belongs to the thiolase-like superfamily. OleA family. FUNCTION: Involved in olefin biosynthesis. Catalyzes a non-decarboxylative head-to-head Claisen condensation of two acyl-CoA molecules, generating an (R)-2-alkyl-3-oxoalkanoate. Is active with fatty acyl-CoA substrates that ranged from C(8) to C(16) in length, and is the most active with palmitoyl-CoA and myristoyl-CoA. KEYWORDS: 3D-structure;Acyltransferase;Cytoplasm;Manganese;Metal-binding;Reference proteome;Transferase SUBCELLULAR LOCATION: Cytoplasm " Q8PZ67,"PROTEIN NAMES: F(420)H(2) dehydrogenase subunit F (Coenzyme F420 oxidoreductase (ferredoxin)) (F(420)H(2)-dependent phenazine dehydrogenase subunit F) (F(420)H(2)-dependent phenazine oxidoreductase subunit F) (FPO subunit F) (Methanophenazine hydrogenase subunit F) (Methanosarcina-phenazine hydrogenase subunit F) ORGANISM: Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia) FUNCTION: Component of the F(420)H(2) dehydrogenase (FPO complex) which is part of the energy-conserving F(420)H(2):heterodisulfide oxidoreductase system. The membrane-bound electron transfer system of the complex plays an important role in the metabolism of methylotrophic methanogens when the organisms grow on methanol or methylamines. Catalyzes the oxidation of methanophenazine to dihydromethanophenazine. It shuttles electrons from F(420)H(2), via FAD and iron-sulfur (Fe-S) centers, to methanophenazine (an electron carrier in the membrane). It couples the redox reaction to proton translocation (for every two electrons transferred, two hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient. It also catalyzes the oxidation of F(420)H(2) with quinones such as 2,3-dimethyl-1,4-naphthoquinone, 2-methyl-1,4-naphthoquinone and tetramethyl-p-benzoquinone. Might have a dual function, acting as an electron input module when connected to the membrane integral Fpo complex, or as a soluble single subunit, being involved in the reoxydation of reduced ferredoxin in the cytoplasm. KEYWORDS: 2Fe-2S;Cytoplasm;Electron transport;FAD;Flavoprotein;Iron;Iron-sulfur;Membrane;Metal-binding;Methanogenesis;Methanol utilization;Oxidoreductase;Repeat;Transport SUBCELLULAR LOCATION: Membrane; Peripheral membrane protein Cytoplasm " Q8QG61,"PROTEIN NAMES: Cryptochrome-1 PROTEIN FAMILY: DNA photolyase class-1 family ORGANISM: Gallus gallus (Chicken) SIMILARITY: Belongs to the DNA photolyase class-1 family. FUNCTION: Transcriptional repressor which forms a core component of the circadian clock. The circadian clock, an internal time-keeping system, regulates various physiological processes through the generation of approximately 24 hour circadian rhythms in gene expression, which are translated into rhythms in metabolism and behavior. It is derived from the Latin roots 'circa' (about) and 'diem' (day) and acts as an important regulator of a wide array of physiological functions including metabolism, sleep, body temperature, blood pressure, endocrine, immune, cardiovascular, and renal function. Consists of two major components: the central clock, residing in the suprachiasmatic nucleus (SCN) of the brain, and the peripheral clocks that are present in nearly every tissue and organ system. Both the central and peripheral clocks can be reset by environmental cues, also known as Zeitgebers (German for 'timegivers'). The predominant Zeitgeber for the central clock is light, which is sensed by retina and signals directly to the SCN. The central clock entrains the peripheral clocks through neuronal and hormonal signals, body temperature and feeding-related cues, aligning all clocks with the external light/dark cycle. Circadian rhythms allow an organism to achieve temporal homeostasis with its environment at the molecular level by regulating gene expression to create a peak of protein expression once every 24 hours to control when a particular physiological process is most active with respect to the solar day. Transcription and translation of core clock components (CLOCK, NPAS2, BMAL1, BMAL2, PER1, PER2, PER3, CRY1 and CRY2) plays a critical role in rhythm generation, whereas delays imposed by post-translational modifications (PTMs) are important for determining the period (tau) of the rhythms (tau refers to the period of a rhythm and is the length, in time, of one complete cycle). A diurnal rhythm is synchronized with the day/night cycle, while the ultradian and infradian rhythms have a period shorter and longer than 24 hours, respectively. Disruptions in the circadian rhythms contribute to the pathology of cardiovascular diseases, cancer, metabolic syndromes and aging. A transcription/translation feedback loop (TTFL) forms the core of the molecular circadian clock mechanism. Transcription factors, CLOCK or NPAS2 and BMAL1 or BMAL2, form the positive limb of the feedback loop, act in the form of a heterodimer and activate the transcription of core clock genes and clock-controlled genes (involved in key metabolic processes), harboring E-box elements (5'-CACGTG-3') within their promoters. The core clock genes: PER1/2/3 and CRY1/2 which are transcriptional repressors form the negative limb of the feedback loop and interact with the CLOCK|NPAS2-BMAL1|BMAL2 heterodimer inhibiting its activity and thereby negatively regulating their own expression. This heterodimer also activates nuclear receptors NR1D1/2 and RORA/B/G, which form a second feedback loop and which activate and repress BMAL1 transcription, respectively. CRY1 and CRY2 have redundant functions but also differential and selective contributions at least in defining the pace of the SCN circadian clock and its circadian transcriptional outputs. More potent transcriptional repressor in cerebellum and liver than CRY2, though more effective in lengthening the period of the SCN oscillator. On its side, CRY2 seems to play a critical role in tuning SCN circadian period by opposing the action of CRY1. With CRY2, is dispensable for circadian rhythm generation but necessary for the development of intercellular networks for rhythm synchrony. Capable of translocating circadian clock core proteins such as PER proteins to the nucleus (By similarity). Interacts with CLOCK-BMAL1 independently of PER proteins and is found at CLOCK-BMAL1-bound sites, suggesting that CRY may act as a molecular gatekeeper to maintain CLOCK-BMAL1 in a poised and repressed state until the proper time for transcriptional activation (By similarity). Represses CLOCK-BMAL1-mediated transcriptional activation. KEYWORDS: Biological rhythms;Chromophore;Cytoplasm;FAD;Flavoprotein;Nucleotide-binding;Nucleus;Photoreceptor protein;Receptor;Reference proteome;Repressor;Sensory transduction;Transcription;Transcription regulation SUBCELLULAR LOCATION: Cytoplasm Nucleus Note=Translocated to the nucleus through interaction with other Clock proteins such as PER2 or BMAL1. " Q8QZV7,"PROTEIN NAMES: Integrator complex subunit 13 (Cell cycle regulator Mat89Bb homolog) (Protein asunder homolog) (Spermatogenesis-associated protein 30) PROTEIN FAMILY: Asunder family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the asunder family. FUNCTION: Crucial regulator of the mitotic cell cycle and development. At prophase, required for dynein anchoring to the nuclear envelope important for proper centrosome-nucleus coupling. At G2/M phase, may be required for proper spindle formation and execution of cytokinesis. Probable component of the Integrator (INT) complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. KEYWORDS: Cell cycle;Cell division;Cytoplasm;Isopeptide bond;Mitosis;Nucleus;Phosphoprotein;Reference proteome;Ubl conjugation SUBCELLULAR LOCATION: Nucleus Cytoplasm Note=Nuclear location is required for recruitment of dynein motors to nuclear envelope at G2/M. " Q8R050,"PROTEIN NAMES: Eukaryotic peptide chain release factor GTP-binding subunit ERF3A (Eukaryotic peptide chain release factor subunit 3a) (eRF3a) (G1 to S phase transition protein 1 homolog) PROTEIN FAMILY: TRAFAC class translation factor GTPase superfamily, Classic translation factor GTPase family, ERF3 subfamily ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. ERF3 subfamily. FUNCTION: GTPase component of the eRF1-eRF3-GTP ternary complex, a ternary complex that mediates translation termination in response to the termination codons UAA, UAG and UGA. GSPT1/ERF3A mediates ETF1/ERF1 delivery to stop codons: The eRF1-eRF3-GTP complex binds to a stop codon in the ribosomal A-site. GTP hydrolysis by GSPT1/ERF3A induces a conformational change that leads to its dissociation, permitting ETF1/ERF1 to accommodate fully in the A-site. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Required for SHFL-mediated translation termination which inhibits programmed ribosomal frameshifting (-1PRF) of mRNA from viruses and cellular genes. KEYWORDS: 3D-structure;Alternative splicing;Direct protein sequencing;GTP-binding;Hydrolase;Nonsense-mediated mRNA decay;Nucleotide-binding;Protein biosynthesis;Reference proteome " Q8R0A6,PROTEIN NAMES: V-set and transmembrane domain-containing protein 2A ORGANISM: Mus musculus (Mouse) FUNCTION: Plays a role in the regulation of the early stage of white and brown preadipocyte cell differentiation. Promotes adipogenic commitment of preadipocytes by increasing gene expression of the transcription factor PPARG in a BMP4-dependent signaling pathway. KEYWORDS: Differentiation;Disulfide bond;Glycoprotein;Immunoglobulin domain;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted Note=Secreted by adipose precursor cells. Not detected in the nucleus (By similarity). Q8R0C3,PROTEIN NAMES: Testis anion transporter 1 (Anion exchange transporter) (Solute carrier family 26 member 8) PROTEIN FAMILY: SLC26A/SulP transporter family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the SLC26A/SulP transporter (TC 2.A.53) family. FUNCTION: Antiporter that mediates the exchange of sulfate and oxalate against chloride ions across a membrane. Stimulates anion transport activity of CFTR (By similarity). May cooperate with CFTR in the regulation of chloride and bicarbonate ions fluxes required for activation of the ADCY10/PKA pathway during sperm motility and sperm capacitation. May play a role in sperm tail differentiation and motility and hence male fertility. KEYWORDS: Alternative splicing;Anion exchange;Developmental protein;Differentiation;Glycoprotein;Ion transport;Meiosis;Membrane;Reference proteome;Spermatogenesis;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Membrane ; Multi-pass membrane protein Note=Located at the annulus ring structure within the sperm cell. Q8R0K2,"PROTEIN NAMES: E3 ubiquitin-protein ligase TRIM31 (Tripartite motif-containing protein 31) PROTEIN FAMILY: TRIM/RBCC family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the TRIM/RBCC family. FUNCTION: E3 ubiquitin-protein ligase that acts as a regulator of antiviral immune response and inflammation by mediating ubiquitination of substrates. Acts as a regulator of innate immune defense against viruses by mediating 'Lys-63'-linked ubiquitination of MAVS, promoting MAVS polymerization and formation of three-stranded helical filaments on mitochondria. Acts as a negative regulator of the NLRP3 inflammasome by catalyzing 'Lys-48'-linked ubiquitination of NLRP3, leading to its degradation. Regulator of Src-induced anchorage independent cell growth. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Antiviral defense;Coiled coil;Cytoplasm;Immunity;Inflammatory response;Innate immunity;Metal-binding;Mitochondrion;Reference proteome;Transferase;Ubl conjugation;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cytoplasm Mitochondrion Note=Predominantly expressed in the cytoplasm but a fraction is associated with the mitochondria. " Q8R1R3,"PROTEIN NAMES: StAR-related lipid transfer protein 7, mitochondrial (START domain-containing protein 7) (StARD7) ORGANISM: Mus musculus (Mouse) FUNCTION: May play a protective role in mucosal tissues by preventing exaggerated allergic responses. KEYWORDS: Coiled coil;Mitochondrion;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Mitochondrion " Q8R1W8,"PROTEIN NAMES: Interphotoreceptor matrix proteoglycan 1 (Interphotoreceptor matrix proteoglycan of 150 kDa) (IPM-150) (Sialoprotein associated with cones and rods) ORGANISM: Mus musculus (Mouse) FUNCTION: Chondroitin sulfate-, heparin- and hyaluronan-binding protein (By similarity). May serve to form a basic macromolecular scaffold comprising the insoluble interphotoreceptor matrix (By similarity). KEYWORDS: Alternative splicing;Cell projection;Extracellular matrix;Glycoprotein;Heparin-binding;Hyaluronic acid;Receptor;Reference proteome;Repeat;Secreted;Sialic acid;Signal SUBCELLULAR LOCATION: Cell projection, cilium, photoreceptor outer segment Secreted, extracellular space, extracellular matrix, interphotoreceptor matrix Photoreceptor inner segment " Q8R1X6,"PROTEIN NAMES: Spartin ORGANISM: Mus musculus (Mouse) FUNCTION: May be implicated in endosomal trafficking, or microtubule dynamics, or both. Participates in cytokinesis. KEYWORDS: Acetylation;Alternative splicing;Cytoplasm;Isopeptide bond;Phosphoprotein;Reference proteome;Ubl conjugation SUBCELLULAR LOCATION: Cytoplasm Midbody Note=Transiently associated with endosomes. Colocalized with IST1 to the ends of Flemming bodies during cytokinesis. " Q8R316,PROTEIN NAMES: HMG box-containing protein 1 (HMG box transcription factor 1) (High mobility group box transcription factor 1) ORGANISM: Mus musculus (Mouse) FUNCTION: Transcriptional repressor that binds to the promoter region of target genes. Plays a role in the regulation of the cell cycle and of the Wnt pathway. Binds preferentially to the sequence 5'-TTCATTCATTCA-3'. Binding to the histone H1.0 promoter is enhanced by interaction with RB1. Disrupts the interaction between DNA and TCF4 (By similarity). KEYWORDS: 3D-structure;Alternative splicing;DNA-binding;Nucleus;Reference proteome;Repressor;Transcription;Transcription regulation;Ubl conjugation;Wnt signaling pathway SUBCELLULAR LOCATION: Nucleus Q8R4E9,"PROTEIN NAMES: DNA replication factor Cdt1 (Double parked homolog) (DUP) (Retroviral insertion site 2 protein) PROTEIN FAMILY: Cdt1 family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the Cdt1 family. FUNCTION: Required for both DNA replication and mitosis. DNA replication licensing factor, required for pre-replication complex assembly. Cooperates with CDC6 and the origin recognition complex (ORC) during G1 phase of the cell cycle to promote the loading of the mini-chromosome maintenance (MCM) complex onto DNA to generate pre-replication complexes (pre-RC). Required also for mitosis by promoting stable kinetochore-microtubule attachments (By similarity). Potential oncogene. KEYWORDS: 3D-structure;Cell cycle;Centromere;Chromosome;DNA replication;DNA-binding;Kinetochore;Nucleus;Phosphoprotein;Proto-oncogene;Reference proteome;Ubl conjugation SUBCELLULAR LOCATION: Nucleus Chromosome, centromere, kinetochore Note=Transiently localizes to kinetochores during prometaphase and metaphase. " Q8R4F1,"PROTEIN NAMES: Netrin-G2 (Laminet-2) ORGANISM: Mus musculus (Mouse) FUNCTION: Involved in controlling patterning and neuronal circuit formation at the laminar, cellular, subcellular and synaptic levels. Promotes neurite outgrowth of both axons and dendrites. KEYWORDS: Alternative splicing;Cell membrane;Developmental protein;Differentiation;Disulfide bond;Glycoprotein;GPI-anchor;Laminin EGF-like domain;Lipoprotein;Membrane;Neurogenesis;Reference proteome;Repeat;Signal SUBCELLULAR LOCATION: Cell membrane ; Lipid-anchor, GPI-anchor ; Extracellular side " Q8R4I7,"PROTEIN NAMES: Neuropilin and tolloid-like protein 1 (Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 1) ORGANISM: Mus musculus (Mouse) FUNCTION: Involved in the development and/or maintenance of neuronal circuitry. Accessory subunit of the neuronal N-methyl-D-aspartate receptor (NMDAR) critical for maintaining the abundance of GRIN2A-containing NMDARs in the postsynaptic density. Regulates long-term NMDA receptor-dependent synaptic plasticity and cognition, at least in the context of spatial learning and memory. KEYWORDS: Cell membrane;Disulfide bond;Glycoprotein;Membrane;Phosphoprotein;Postsynaptic cell membrane;Receptor;Reference proteome;Repeat;Signal;Synapse;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Single-pass type I membrane protein Postsynaptic density membrane Note=Component of the postsynaptic density (PSD) of excitatory synapses. " Q8R4S8,"PROTEIN NAMES: Cytokine receptor-like factor 2 (Thymic stromal lymphopoietin protein receptor) (TSLP receptor) PROTEIN FAMILY: Type I cytokine receptor family, Type 5 subfamily ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the type I cytokine receptor family. Type 5 subfamily. FUNCTION: Receptor for thymic stromal lymphopoietin (TSLP). Forms a functional complex with TSLP and IL7R which is capable of stimulating cell proliferation through activation of STAT3 and STAT5 (By similarity). Also activates JAK2 (By similarity). Implicated in the development of the hematopoietic system. KEYWORDS: Disulfide bond;Glycoprotein;Membrane;Receptor;Reference proteome;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Single-pass type I membrane protein " Q8R4T9,"PROTEIN NAMES: Urea transporter 2 (Solute carrier family 14 member 2) (Urea transporter, kidney) PROTEIN FAMILY: Urea transporter family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the urea transporter family. FUNCTION: [Isoform A1]: Mediates the transport of urea driven by a concentration gradient across the cell membrane of the renal inner medullary collecting duct which is critical to the urinary concentrating mechanism.; FUNCTION: [Isoform A2]: Mediates the transport of urea driven by a concentration gradient across the cell membrane of the renal inner medullary collecting duct which is critical to the urinary concentrating mechanism.; FUNCTION: [Isoform A3]: Mediates the transport of urea driven by a concentration gradient across the cell membrane of the renal inner medullary collecting duct which is critical to the urinary concentrating mechanism.; FUNCTION: [Isoform A5]: Mediates the transport of urea driven by a concentration gradient across the cell membrane. Implicated in the urea movement across the blood-testis barrier and does not translocate water. KEYWORDS: Alternative splicing;Cell membrane;Glycoprotein;Membrane;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Apical cell membrane ; Multi-pass membrane protein Basolateral cell membrane ; Multi-pass membrane protein " Q8R4X1,"PROTEIN NAMES: Alkaline ceramidase 1 (AlkCDase 1) (Alkaline CDase 1) (maCER1) (Acylsphingosine deacylase 3) (N-acylsphingosine amidohydrolase 3) PROTEIN FAMILY: Alkaline ceramidase family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the alkaline ceramidase family. FUNCTION: Endoplasmic reticulum ceramidase that catalyzes the hydrolysis of ceramides into sphingosine and free fatty acids at alkaline pH. Ceramides, sphingosine, and its phosphorylated form sphingosine-1-phosphate are bioactive lipids that mediate cellular signaling pathways regulating several biological processes including cell proliferation, apoptosis and differentiation. Exhibits a strong substrate specificity towards the natural stereoisomer of ceramides with D-erythro-sphingosine as a backbone and has a higher activity towards very long-chain unsaturated fatty acids like the C24:1-ceramide. May also hydrolyze dihydroceramides to produce dihydrosphingosine (By similarity). ACER1 is a skin-specific ceramidase that regulates the levels of ceramides, sphingosine and sphingosine-1-phosphate in the epidermis, mediates the calcium-induced differentiation of epidermal keratinocytes and more generally plays an important role in skin homeostasis. PATHWAY: Lipid metabolism; sphingolipid metabolism. KEYWORDS: Calcium;Endoplasmic reticulum;Hydrolase;Lipid metabolism;Membrane;Metal-binding;Reference proteome;Sphingolipid metabolism;Transmembrane;Transmembrane helix;Zinc SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein " Q8R4Z4,PROTEIN NAMES: ETS translocation variant 3 (ETS domain transcriptional repressor PE1) (PE-1) (Mitogenic Ets transcriptional suppressor) PROTEIN FAMILY: ETS family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the ETS family. FUNCTION: Transcriptional repressor that contribute to growth arrest during terminal macrophage differentiation by repressing target genes involved in Ras-dependent proliferation. Represses MMP1 promoter activity. KEYWORDS: Acetylation;DNA-binding;Isopeptide bond;Nucleus;Phosphoprotein;Reference proteome;Repressor;Transcription;Transcription regulation;Ubl conjugation SUBCELLULAR LOCATION: Nucleus Q8R550,"PROTEIN NAMES: SH3 domain-containing kinase-binding protein 1 (Regulator of ubiquitous kinase) (Ruk) (SH3-containing, expressed in tumorigenic astrocytes) ORGANISM: Mus musculus (Mouse) FUNCTION: Adapter protein involved in regulating diverse signal transduction pathways. Involved in the regulation of endocytosis and lysosomal degradation of ligand-induced receptor tyrosine kinases, including EGFR and MET/hepatocyte growth factor receptor, through an association with CBL and endophilins. The association with CBL, and thus the receptor internalization, may be inhibited by an interaction with PDCD6IP and/or SPRY2. Involved in regulation of ligand-dependent endocytosis of the IgE receptor. Attenuates phosphatidylinositol 3-kinase activity by interaction with its regulatory subunit (By similarity). May be involved in regulation of cell adhesion; promotes the interaction between TTK2B and PDCD6IP. May be involved in the regulation of cellular stress response via the MAPK pathways through its interaction with MAP3K4. Is involved in modulation of tumor necrosis factor mediated apoptosis. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape and migration (By similarity). Has an essential role in the stimulation of B cell activation (By similarity). KEYWORDS: 3D-structure;Alternative splicing;Apoptosis;Cell junction;Coiled coil;Cytoplasm;Cytoplasmic vesicle;Cytoskeleton;Endocytosis;Membrane;Phosphoprotein;Reference proteome;Repeat;SH3 domain;SH3-binding;Synapse;Synaptosome;Ubl conjugation SUBCELLULAR LOCATION: Cytoplasm Cytoplasm, cytoskeleton Cytoplasmic vesicle membrane ; Peripheral membrane protein Synapse, synaptosome Cell junction, focal adhesion Note=Localized in endocytic vesicles containing clustered receptors. Colocalizes with ASAP1 in vesicular structures. Colocalized with actin microfilaments and focal adhesions (By similarity). Colocalized with MAGI2 in synaptosomes (By similarity). Translocation to EGFR containing vesicles upon EGF stimulation is inhibited in the presence of SH3KBP1. Colocalizes with ZFP36 in the cytoplasm (By similarity). " Q8RM03,"PROTEIN NAMES: Acetone carboxylase alpha subunit PROTEIN FAMILY: Oxoprolinase family ORGANISM: Xanthobacter autotrophicus (strain ATCC BAA-1158 / Py2) SIMILARITY: Belongs to the oxoprolinase family. FUNCTION: Catalyzes the carboxylation of acetone to form acetoacetate. Has a reduced activity on butanone, and no activity on 2-pentatone, 3-pentatone, 2-hexanone, chloroacetone, pyruvate, phosphoenolpyruvate, acetaldehyde, propionaldehyde and propylene oxide. KEYWORDS: 3D-structure;ATP-binding;Ligase;Nucleotide-binding;Reference proteome " Q8RM04,"PROTEIN NAMES: Acetone carboxylase beta subunit PROTEIN FAMILY: Oxoprolinase family ORGANISM: Xanthobacter autotrophicus (strain ATCC BAA-1158 / Py2) SIMILARITY: Belongs to the oxoprolinase family. FUNCTION: Catalyzes the carboxylation of acetone to form acetoacetate. Has a reduced activity on butanone, and no activity on 2-pentatone, 3-pentatone, 2-hexanone, chloroacetone, pyruvate, phosphoenolpyruvate, acetaldehyde, propionaldehyde and propylene oxide. KEYWORDS: 3D-structure;ATP-binding;Ligase;Nucleotide-binding;Reference proteome " Q8RNT4,"PROTEIN NAMES: Linoleate 9/13-lipoxygenase (Oleate 10S-lipoxygenase) PROTEIN FAMILY: Lipoxygenase family ORGANISM: Pseudomonas aeruginosa SIMILARITY: Belongs to the lipoxygenase family. FUNCTION: In presence of oxygen, converts linoleate into (9S)-hydroperoxy-10,12-octadecenoate (9HPOD), which spontaneously decomposes to the corresponding 9-hydroxy-10,12-octadecenoate (9HOD), and into 13-hydroperoxy-9,11-octadecenoate (13HPOD) which spontaneously decomposes to the corresponding 13-hydroxy-9,11-octadecenoate (13HOD). Also active on linolenate. To a lesser extent, is also able to convert oleate into (10S)-hydroperoxy-8E-octadecenoate, which spontaneously decomposes to the corresponding 10-hydroxy-8E-octadecenoate. Is almost not active on arachidonate. KEYWORDS: 3D-structure;Dioxygenase;Iron;Metal-binding;Oxidoreductase;Periplasm;Signal SUBCELLULAR LOCATION: Periplasm MISCELLANEOUS: In vitro, under anaerobic conditions, does not transform linoleate or oleate into the corresponding hydroxy derivative." Q8RUF8,"PROTEIN NAMES: Omega-amidase, chloroplastic (Nitrilase-like protein 3) PROTEIN FAMILY: Nitrilase superfamily, NIT1/NIT2 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the nitrilase superfamily. NIT1/NIT2 family. FUNCTION: Omega-amidase involved in the metabolism of asparagine. Probably also closely coupled with glutamine transamination in the methionine salvage cycle. Can use alpha-ketosuccinamate and alpha-hydroxysuccinamate as substrates, producing respectively oxaloacetate and malate, or alpha-ketoglutaramate, producing alpha-ketoglutarate. KEYWORDS: Acetylation;Alternative splicing;Chloroplast;Hydrolase;Plastid;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast " Q8RUS5,"PROTEIN NAMES: Autophagy-related protein 9 (AtAPG9) PROTEIN FAMILY: ATG9 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the ATG9 family. FUNCTION: Phospholipid scramblase involved in autophagy by mediating autophagosomal membrane expansion. Cycles between the preautophagosomal structure/phagophore assembly site (PAS) and the cytoplasmic vesicle pool and supplies membrane for the growing autophagosome. Lipid scramblase activity plays a key role in preautophagosomal structure/phagophore assembly by distributing the phospholipids that arrive through ATG2 from the cytoplasmic to the luminal leaflet of the bilayer, thereby driving autophagosomal membrane expansion (By similarity). In addition to autophagy, also plays a role in necrotic cell death (By similarity). Plays an essential role in plant nutrient recycling. KEYWORDS: Autophagy;Lipid transport;Membrane;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Preautophagosomal structure membrane ; Multi-pass membrane protein " Q8RWE8,"PROTEIN NAMES: GDP-L-galactose phosphorylase 1 (Protein VITAMIN C DEFECTIVE 2) PROTEIN FAMILY: GDPGP1 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the GDPGP1 family. FUNCTION: Catalyzes a reaction of the Smirnoff-Wheeler pathway, the major route to ascorbate biosynthesis in plants. Acts as a phosphorylase rather than as a transferase. Uses preferentially GDP-L-galactose and GDP-D-glucose as substrates. Lower activity with GDP-L-fucose, very low activity with GDP-D-mannose, and no activity with UDP-D-glucose, UDP-D-galactose or ADP-D-glucose. Highly specific for inorganic phosphate as the guanylyl acceptor. PATHWAY: Cofactor biosynthesis; L-ascorbate biosynthesis via GDP-alpha-D-mannose pathway; L-ascorbate from GDP-alpha-D-mannose: step 2/5. KEYWORDS: Ascorbate biosynthesis;Cytoplasm;Guanine-nucleotide releasing factor;Hydrolase;Nucleotide-binding;Nucleotidyltransferase;Nucleus;Reference proteome;Transferase SUBCELLULAR LOCATION: Cytoplasm Nucleus " Q8RWG1,"PROTEIN NAMES: Protein ACTIVITY OF BC1 COMPLEX KINASE 1, chloroplastic (ABC1-LIKE KINASE 1) (Protein ABC1-LIKE KINASE RELATED TO CHLOROPHYLL DEGRADATION AND OXIDATIVE STRESS 1) (AtACDO1) (Protein BLEACHING AND DWARF IN RED LIGHT 1) (Protein PROTON GRADIENT REGULATION 6) PROTEIN FAMILY: Protein kinase superfamily, ADCK protein kinase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the protein kinase superfamily. ADCK protein kinase family. FUNCTION: Kinase that can phosphorylate the tocopherol cyclase VTE1, a key enzyme of tocopherol (vitamin E) metabolism and involved in the recycling of oxidated alpha-tocopherol quinone, possibly stabilizing it at plastoglobules. Regulates also plastoglobule protein composition. Prevents photodamage of chloroplasts under continuous red light, thus working in opposition to ABC1K3. Together with ABC1K1, contributes to plastoglobule (PG) function in prenyl-lipid metabolism, stress response, and thylakoid remodeling. Involved in chlorophyll degradation and in the maintenance of the number of chlorophyll-binding photosynthetic thylakoid membranes. Ensures photosynthetic electron transport by regulating the homeostasis of plastoquinone, beta-carotene and xanthophyll lutein, as well as membrane antioxidant tocopherol metabolism. Seems to affect specifically stability or turnover of D1 protein, product of psbA, one of the four core subunits of the photosystem II (PSII). Required for photooxidative stress responses, including the induction of oxidative stress response genes (e.g. FSD1, CSD1, CAT1, and UTG71C1), to prevent photosystem II core and chlorophyll degradations. KEYWORDS: Alternative splicing;ATP-binding;Chloroplast;Kinase;Nucleotide-binding;Plastid;Reference proteome;Stress response;Transferase;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast, plastoglobule Plastid, chloroplast " Q8RWK8,"PROTEIN NAMES: Coilin (Atcoilin) PROTEIN FAMILY: Coilin family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the coilin family. FUNCTION: Probable component of nuclear coiled bodies, also known as Cajal bodies or CBs, which are involved in the modification and assembly of nucleoplasmic snRNPs (Probable). Required for CBs formation. Binds snRNAs and non-specific artificial RNA via the N-terminal part of the NOD domain and via the NLS2 region (212-282) of the IDD domain. The two sites are able to function independently and provide effective RNA-binding in a non-cooperative manner. KEYWORDS: Nucleus;Phosphoprotein;Reference proteome;RNA-binding SUBCELLULAR LOCATION: Nucleus Nucleus, Cajal body " Q8RWL4,"PROTEIN NAMES: Increased DNA methylation 2 (Alpha-crystallin domain-containing protein 39.4) (AtAcd39.4) (Protein ROS5) (Repressor of silencing 5) PROTEIN FAMILY: Small heat shock protein (HSP20) family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the small heat shock protein (HSP20) family. FUNCTION: Prevents DNA hypermethylation and transcriptional silencing of transgenes and of some endogenous genes. May act as a molecular chaperone of IDM1, regulating its H3K18 acetylation activity. KEYWORDS: Alternative splicing;Nucleus;Reference proteome;Stress response;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus, nucleoplasm Note=Colocalizes with IDM1 within nucleoplasmic and nucleolar foci. " Q8RWT8,"PROTEIN NAMES: Serine--tRNA ligase, chloroplastic/mitochondrial (AtSRS) (Protein OVULE ABORTION 7) (Seryl-tRNA synthetase) (SerRS) (Seryl-tRNA(Ser/Sec) synthetase) PROTEIN FAMILY: Class-II aminoacyl-tRNA synthetase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family. FUNCTION: Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec) (By similarity). PATHWAY: Aminoacyl-tRNA biosynthesis; selenocysteinyl-tRNA(Sec) biosynthesis; L-seryl-tRNA(Sec) from L-serine and tRNA(Sec): step 1/1. KEYWORDS: Alternative splicing;Aminoacyl-tRNA synthetase;ATP-binding;Chloroplast;Ligase;Mitochondrion;Nucleotide-binding;Plastid;Protein biosynthesis;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast Mitochondrion " Q8RWW0,"PROTEIN NAMES: Receptor-like serine/threonine-protein kinase ALE2 (Protein ABNORMAL LEAF SHAPE 2) PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. FUNCTION: Required during the differentiation of the protoderm into shoots epidermis and cuticle. KEYWORDS: ATP-binding;Cell membrane;Developmental protein;Differentiation;Glycoprotein;Kinase;Membrane;Nucleotide-binding;Phosphoprotein;Receptor;Reference proteome;Serine/threonine-protein kinase;Signal;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein " Q8RX79,"PROTEIN NAMES: Peptide chain release factor APG3, chloroplastic (Chloroplast ribosome release factor 1) (AtcpRF1) (Protein ALBINO AND PALE GREEN 3) PROTEIN FAMILY: Prokaryotic/mitochondrial release factor family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the prokaryotic/mitochondrial release factor family. FUNCTION: May direct the termination of translation in response to the peptide chain termination codon UAG. Required for normal chloroplast development and plays essential roles in the termination of translation in plastids. KEYWORDS: Chloroplast;Growth regulation;Plastid;Protein biosynthesis;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast " Q8RXF8,"PROTEIN NAMES: Mitochondrial Rho GTPase 1 (AtMIRO1) (Miro-related GTPase 1) PROTEIN FAMILY: Mitochondrial Rho GTPase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the mitochondrial Rho GTPase family. FUNCTION: Mitochondrial GTPase required to maintain proper development, morphology and intracellular distribution of mitochondria, which in turn are essential for the progress of embryonic cell division, development of haploid male and female gametes, and pollen tube growth. KEYWORDS: Calcium;GTP-binding;Hydrolase;Membrane;Metal-binding;Mitochondrion;Mitochondrion outer membrane;Nucleotide-binding;Phosphoprotein;Reference proteome;Repeat;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Mitochondrion outer membrane ; Single-pass type IV membrane protein " Q8RXN6,"PROTEIN NAMES: U4/U6 small nuclear ribonucleoprotein Prp31 homolog (Pre-mRNA-processing factor 31 homolog) (Protein EMBRYO DEFECTIVE 1220) PROTEIN FAMILY: PRP31 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the PRP31 family. FUNCTION: Involved in pre-mRNA splicing. Required for the assembly of the U4/U5/U6 tri-snRNP complex, one of the building blocks of the spliceosome (By similarity). Functions in association with STA1 and ZOP1 in spliceosome dynamics and pre-mRNA splicing. Required for transcriptional regulation and pre-mRNA splicing of cold-responsive genes, such as LTI78/RD29A, KIN2/COR6.6 or COR15A, especially under cold stress. May play a role in stress response. Involved in transcriptional gene silencing of endogenous transposable elements, independently of the RNA-directed DNA methylation (RdDM) pathway. Seems not to participate in the small RNA biogenesis of the RdDM pathway. KEYWORDS: mRNA processing;mRNA splicing;Nucleus;Reference proteome;Ribonucleoprotein;RNA-binding;Spliceosome;Stress response SUBCELLULAR LOCATION: Nucleus Nucleus, Cajal body " Q8RXU6,"PROTEIN NAMES: WD repeat-containing protein PCN (Protein POPCORN) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Involved in auxin signaling pathway. Required for embryo development and meristem organization. Functions in the auxin pathway, integrating auxin signaling in the organization and maintenance of the shoot apical meristem (SAM) and root apical meristem (RAM). KEYWORDS: Developmental protein;Nucleus;Reference proteome;Repeat;WD repeat SUBCELLULAR LOCATION: Nucleus " Q8RY00,PROTEIN NAMES: Sialyltransferase-like protein 2 PROTEIN FAMILY: Glycosyltransferase 29 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the glycosyltransferase 29 family. FUNCTION: May be involved in the transfer of 2-keto-3-deoxy-D-lyxo-heptulosaric acid (Dha) and/or 2-keto-3-deoxy-D-manno-octulosonic acid (Kdo) on the homogalacturonan backbone of rhamnogalacturonan-II. Required for efficient pollen grain germination and pollen tube elongation. Does not possess sialyltransferase activity in vitro. KEYWORDS: Alternative splicing;Glycoprotein;Glycosyltransferase;Golgi apparatus;Membrane;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus membrane ; Single-pass type II membrane protein Q8RY59,"PROTEIN NAMES: Inactive poly [ADP-ribose] polymerase RCD1 (Protein RADICAL-INDUCED CELL DEATH 1) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Inactive ADP-ribosyltransferase that functions with SRO1 to regulate oxidative stress, hormonal and developmental responses. Required for embryogenesis, vegetative and reproductive development, and abiotic stress responses. May regulate several stress-responsive genes. Seems to play a larger developmental role than SRO1. Does not bind NAD in vitro. KEYWORDS: 3D-structure;Alternative splicing;Developmental protein;Host-virus interaction;Nucleus;Reference proteome;Stress response SUBCELLULAR LOCATION: Nucleus matrix Note=Speckle-like pattern. MISCELLANEOUS: Plants overexpressing RCD1 show a weak rcd1 mutant phenotype.; MISCELLANEOUS: [Isoform 2]: May be due to a competing acceptor splice site." Q8RY73,PROTEIN NAMES: NAD-capped RNA hydrolase DXO1 (DeNADding enzyme DXO1) PROTEIN FAMILY: DXO/Dom3Z family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the DXO/Dom3Z family. FUNCTION: Decapping enzyme for NAD-capped RNAs: specifically hydrolyzes the nicotinamide adenine dinucleotide (NAD) cap from a subset of RNAs by removing the entire NAD moiety from the 5'-end of an NAD-capped RNA. The NAD-cap is present at the 5'-end of some RNAs and promotes mRNA decay (By similarity). Its precise role is unclear: may be involved in the connection between RNA turnover and retrograde chloroplast-to-nucleus signaling independently of its deNADding activity. KEYWORDS: 3D-structure;Alternative splicing;Cytoplasm;Hydrolase;Magnesium;Metal-binding;Nuclease;Nucleotide-binding;Nucleus;Phosphoprotein;Reference proteome;RNA-binding SUBCELLULAR LOCATION: Cytoplasm Nucleus Q8S1V1,"PROTEIN NAMES: Chitinase CLP (OsCLP) (Chitinase-like protein) PROTEIN FAMILY: Peptidase A1 family ORGANISM: Oryza sativa subsp. japonica (Rice) SIMILARITY: Belongs to the peptidase A1 family. FUNCTION: Chitinase that possesses antifungal activity. Inhibits the growth of the fungal pathogen Rhizoctonia solani by degrading the fungal cell wall. Does not possess inhibiting activity against fungal endo-1,4-beta-D-xylanases belonging to glycoside hydrolase family 10 (GH10) and family 11 (GH11). Involved in the regulation of plant growth by regulating the intracellular calcium ion concentration in roots. KEYWORDS: Apoplast;Carbohydrate metabolism;Chitin degradation;Glycoprotein;Glycosidase;Growth regulation;Hydrolase;Plant defense;Polysaccharide degradation;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted, extracellular space, apoplast " Q8S3J3,"PROTEIN NAMES: Hydroxyisourate hydrolase (HIU hydrolase) (HIUHase) PROTEIN FAMILY: Glycosyl hydrolase 1 family ORGANISM: Glycine max (Soybean) (Glycine hispida) SIMILARITY: Belongs to the glycosyl hydrolase 1 family. FUNCTION: Involved in the conversion of hydroxyisourate to ureides such as allantoin, the major form of nitrogen transport in legumes. PATHWAY: Purine metabolism; urate degradation; (S)-allantoin from urate: step 2/3. KEYWORDS: Direct protein sequencing;Disulfide bond;Glycoprotein;Hydrolase;Membrane;Peroxisome;Purine metabolism;Reference proteome;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Peroxisome membrane ; Single-pass type I membrane protein " Q8S7E1,"PROTEIN NAMES: Chlorophyllide a oxygenase, chloroplastic (Chlorophyll a oxygenase) (Chlorophyll b synthase) ORGANISM: Oryza sativa subsp. japonica (Rice) FUNCTION: Catalyzes a two-step oxygenase reaction involved in the synthesis of chlorophyll b. Acts specifically on the non-esterified chlorophyllide a and not on chlorophyll a. KEYWORDS: 2Fe-2S;Alternative splicing;Chlorophyll biosynthesis;Chloroplast;Coiled coil;Iron;Iron-sulfur;Membrane;Metal-binding;NADP;Oxidoreductase;Plastid;Reference proteome;Thylakoid;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast membrane; Peripheral membrane protein. Plastid, chloroplast thylakoid membrane ; Peripheral membrane protein " Q8S8S1,"PROTEIN NAMES: Peroxisome biogenesis protein 16 (Peroxin-16) (AtPEX16) (AtPex16p) (Protein SHRUNKEN SEED 1) PROTEIN FAMILY: Peroxin-16 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the peroxin-16 family. FUNCTION: Involved in the formation of peroxisomes, lipid bodies and protein bodies. KEYWORDS: Endoplasmic reticulum;Membrane;Peroxisome;Peroxisome biogenesis;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Peroxisome membrane ; Multi-pass membrane protein Endoplasmic reticulum membrane ; Multi-pass membrane protein Note=Moves through an intermediate compartment from endoplasmic reticulum to pre-existing peroxisomes. MISCELLANEOUS: Travels from the cytosol to peroxisomes via the reticular and perinuclear endoplasmic reticulum (ER) and an ER-peroxisome intermediate compartment (ERPIC)." Q8S8S2,"PROTEIN NAMES: Lysophospholipid acyltransferase LPEAT2 (Lysophosphatidylethanolamine acyltransferase 2) (AtLPEAT2) PROTEIN FAMILY: 1-acyl-sn-glycerol-3-phosphate acyltransferase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family. FUNCTION: Possesses acyl-CoA-dependent lysophospholipid acyltransferase activity with a subset of lysophospholipids as substrates. Exhibits strong acylation activity on lysophosphatidylethanolamine (LPE), and lower activity on lysophosphatidylcholine (LPC) and lysophosphatidylserine (LPS). Exhibits acylation activity on both LPE and LPC. Has a preference for 18:1-LPE over 16:0-LPE as acceptor. Palmitoyl-CoA (16:0-CoA) is a better acyl donor than oleoyl-CoA (18:1-CoA). Among several different acyl-CoA species the best acyl donor is eicosanoyl-CoA (20:0-CoA). Activity is calcium-independent. Its activity is essential for maintaining adequate levels of phosphatidylethanolamine (PE), LPE and LPC in the cells, which is crucial for plant growth regulation. PATHWAY: Lipid metabolism; phospholipid metabolism. KEYWORDS: Acyltransferase;Alternative splicing;Calcium;Endosome;Golgi apparatus;Lipid biosynthesis;Lipid metabolism;Membrane;Metal-binding;Phospholipid biosynthesis;Phospholipid metabolism;Reference proteome;Repeat;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus membrane ; Single-pass membrane protein Late endosome membrane ; Single-pass membrane protein MISCELLANEOUS: The ratio of isoform 2/isoform 1 mRNA is increased about 15-fold in the nonsense mRNA reducing factor mutant upf3-1.; MISCELLANEOUS: [Isoform 2]: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay." Q8S9K3,"PROTEIN NAMES: Zinc finger protein VAR3, chloroplastic (Organelle Zinc finger 1) (Protein VARIEGATED 3) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Probable component of some protein complex required for chloroplast and palisade cell development. Involved in C-to-U editing of chloroplastic RNA. Controls a large number of chloroplastic editing sites. Binds the editing recognition trans-factors PCMP-H51/CRR28 and PCMP-H12/OTP82. KEYWORDS: Chloroplast;Metal-binding;mRNA processing;Plastid;Reference proteome;Repeat;Transit peptide;Zinc;Zinc-finger SUBCELLULAR LOCATION: Plastid, chloroplast Note=Localizes at punctuate loci in chloroplasts. " Q8SQG7,"PROTEIN NAMES: Hyaluronidase-2 (Hyal-2) (Hyaluronoglucosaminidase-2) PROTEIN FAMILY: Glycosyl hydrolase 56 family ORGANISM: Ovis aries (Sheep) SIMILARITY: Belongs to the glycosyl hydrolase 56 family. FUNCTION: Hydrolyzes high molecular weight hyaluronic acid to produce an intermediate-sized product which is further hydrolyzed by sperm hyaluronidase to give small oligosaccharides. Displays very low levels of activity. Associates with and negatively regulates MST1R (By similarity). KEYWORDS: Cell membrane;Disulfide bond;EGF-like domain;Glycoprotein;Glycosidase;GPI-anchor;Host-virus interaction;Hydrolase;Lipoprotein;Membrane;Receptor;Reference proteome;Signal SUBCELLULAR LOCATION: Cell membrane ; Lipid-anchor, GPI-anchor MISCELLANEOUS: Acts as a receptor for the Jaagsiekte sheep retrovirus (JSRV), which induces ovine pulmonary adenocarcinoma. A possible mechanism is that binding to JSRV envelope proteins may liberate the oncogenic factor MST1R that is normally negatively regulated by HYAL2, leading to oncogenic transformation." Q8SX37,"PROTEIN NAMES: CDP-diacylglycerol--inositol 3-phosphatidyltransferase (CDIPT) (Phosphatidylinositol synthase) PROTEIN FAMILY: CDP-alcohol phosphatidyltransferase class-I family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the CDP-alcohol phosphatidyltransferase class-I family. FUNCTION: Catalyzes the biosynthesis of phosphatidylinositol (PtdIns) as well as PtdIns:inositol exchange reaction. May thus act to reduce an excessive cellular PtdIns content (By similarity). The exchange activity is due to the reverse reaction of PtdIns synthase and is dependent on CMP, which is tightly bound to the enzyme (By similarity). Required for the regeneration of the signaling molecule phosphatidylinositol 4,5-bisphosphate (PtdInsP2) from phosphatidic acid (PA) and maintenance of its steady supply during signaling, thus playing an essential role during phospholipase C-mediated transduction. This function is essential in photoreceptors for light-activated recycling of PtdInsP2 during phototransduction. As a key enzyme of the phosphoinositide pathway, indirectly involved in the polarized secretion of basal membrane (BM) proteins in follicle epithelial (FE) cells through promoting PtdInsP2 synthesis in the apical and lateral plasma membranes of FE cells. PtdInsP2 controls the localization of Crag and perhaps the localization and expression of strat, both of which are essential for restricting the secretion of BM proteins to the basal surface. KEYWORDS: Alternative splicing;Cell membrane;Lipid biosynthesis;Lipid metabolism;Magnesium;Manganese;Membrane;Metal-binding;Phospholipid biosynthesis;Phospholipid metabolism;Reference proteome;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Apical cell membrane ; Multi-pass membrane protein Lateral cell membrane ; Multi-pass membrane protein Note=In follicle cells, detected at the apical and lateral regions of the plasma membrane. " Q8SX86,"PROTEIN NAMES: Protein nutcracker ORGANISM: Drosophila melanogaster (Fruit fly) FUNCTION: Functions together with PI31 to control non-apoptotic caspase activation during sperm individualization. Positively regulates PI31 stability. KEYWORDS: Cytoplasm;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Note=In the spermatid, colocalizes with Cul1 at the actin-based individualization complex and the cystic bulge. " Q8T045,"PROTEIN NAMES: O-glucosyltransferase rumi PROTEIN FAMILY: Glycosyltransferase 90 family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the glycosyltransferase 90 family. FUNCTION: Protein O-glucosyltransferase. Catalyzes the reaction that attaches glucose through an O-glycosidic linkage to a conserved serine residue found in the consensus sequence C-X-S-X-[PA]-C in epidermal growth factor-like repeats. Regulates Notch signaling by glucosylating Notch in the ER, glucosylation is required for the correct folding and cleavage of Notch. PATHWAY: Protein modification; protein glycosylation. KEYWORDS: 3D-structure;Disulfide bond;Endoplasmic reticulum;Glycosyltransferase;Notch signaling pathway;Reference proteome;Signal;Transferase SUBCELLULAR LOCATION: Endoplasmic reticulum lumen " Q8T1G4,"PROTEIN NAMES: Lysozyme A (1,4-beta-N-acetylmuramidase A) PROTEIN FAMILY: Dictyostelium lysozyme family ORGANISM: Dictyostelium discoideum (Social amoeba) SIMILARITY: Belongs to the dictyostelium lysozyme family. FUNCTION: Has antibacterial activity against the Gram-positive bacteria B.subtilis, B.megaterium and M.luteus. No antibacterial activity detected against the Gram-positive bacterium S.aureus or against the Gram-negative bacterium E.coli. Lacks chitinase activity. KEYWORDS: Antibiotic;Antimicrobial;Bacteriolytic enzyme;Cytoplasmic vesicle;Direct protein sequencing;Disulfide bond;Glycosidase;Hydrolase;Reference proteome;Signal SUBCELLULAR LOCATION: Cytoplasmic vesicle lumen " Q8T3B9,"PROTEIN NAMES: Transcription factor sem-2 (Sex muscle abnormal protein 2) ORGANISM: Caenorhabditis elegans FUNCTION: Probable transcription factor required for embryogenesis, vulval development and cell fate specification of the postembryonic mesoderm (also known as the M lineage). Specifically, required for the specification of sex myoblast cells and their development into the muscles that are necessary for egg-laying. In addition, may be involved in RME GABAergic motor neuron progenitor cell fate specification. KEYWORDS: Developmental protein;DNA-binding;Nucleus;Reference proteome;Transcription SUBCELLULAR LOCATION: Nucleus " Q8T5S1,"PROTEIN NAMES: Na(+)/H(+) exchanger protein 2 (Na(+)/H(+) antiporter nhx-2) PROTEIN FAMILY: Monovalent cation:proton antiporter 1 (CPA1) transporter family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family. FUNCTION: Na(+)/H(+) antiporter that promotes normal di- or tripeptide transporter function, recovery following the peptide-induced acidification of the intestinal cytoplasm and maintenance of the peptide-dependent intestinal pH homeostasis. Regulator of free fatty acid uptake from the diet together with the dipeptide transporter pept-1. May play a timekeeper role in defecation cycle but is not necessary for pbo-4-dependent proton release. May play a role in the regulation of lifespan independent of the stress response pathway. KEYWORDS: Antiport;Cell membrane;Ion transport;Membrane;Reference proteome;Sodium;Sodium transport;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Apical cell membrane ; Multi-pass membrane protein ; Lumenal side Note=Colocalizes with pept-1 along the apical membrane of the intestinal cells. " Q8T7E0,"PROTEIN NAMES: Endoplasmin homolog (Glucose-regulated protein 94) (Lipophosphoglycan biosynthetic protein 3) PROTEIN FAMILY: Heat shock protein 90 family ORGANISM: Leishmania donovani SIMILARITY: Belongs to the heat shock protein 90 family. FUNCTION: Molecular chaperone that functions in the processing and transport of secreted proteins (By similarity). Required for the synthesis of lipophosphoglycan (LPG), a cell surface glycoconjugate. Necessary for the attachment of the galactosyl residue to the mannose within the phosphoglycan repeats of the nascent LPG chain. Also required for addition of phosphoglycan to acid phosphatase. Not required for normal growth. Has ATPase activity (By similarity). Binds heparin with micromolar affinity which may facilitate infection of host cells (By similarity). KEYWORDS: ATP-binding;Chaperone;Endoplasmic reticulum;Glycoprotein;Heparin-binding;Nucleotide-binding;Signal SUBCELLULAR LOCATION: Endoplasmic reticulum " Q8T9S7,"PROTEIN NAMES: Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN (Inositol polyphosphate 3-phosphatase) (Pten 3-phosphoinositide phosphatase alpha) PROTEIN FAMILY: PTEN phosphatase protein family ORGANISM: Dictyostelium discoideum (Social amoeba) SIMILARITY: Belongs to the PTEN phosphatase protein family. FUNCTION: Dual-specificity protein phosphatase, dephosphorylating tyrosine-, serine- and threonine-phosphorylated proteins. Also functions as a lipid phosphatase, removing the phosphate in the D3 position of the inositol ring of PtdIns(3,4,5)P3/phosphatidylinositol 3,4,5-trisphosphate, PtdIns(3,4)P2/phosphatidylinositol 3,4-diphosphate and PtdIns3P/phosphatidylinositol 3-phosphate with a preference for PtdIns(3,4,5)P3. Furthermore, this enzyme can also act as a cytosolic inositol 3-phosphatase acting on Ins(1,3,4,5,6)P5/inositol 1,3,4,5,6 pentakisphosphate and possibly Ins(1,3,4,5)P4/1D-myo-inositol 1,3,4,5-tetrakisphosphate (By similarity). Negative regulator of PI3K chemotaxis pathways. Overexpression leads to a suppression of a PI3K-dependent activation of pkbA, and these cells exhibit chemotaxis defects consistent with a reduction in PI3K activity. KEYWORDS: Cell cycle;Cell membrane;Chemotaxis;Cytoplasm;Hydrolase;Lipid metabolism;Lipid-binding;Membrane;Protein phosphatase;Reference proteome SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm. Cytoplasm, cell cortex. Note=Found uniformly on the plasma membrane in unstimulated cells. In response to chemoattractant stimulation, there is a rapid and transient release from the plasma membrane. Constitutively localized in the cortex of polarized cells. " Q8TAE7,"PROTEIN NAMES: Potassium voltage-gated channel subfamily G member 3 (Voltage-gated potassium channel subunit Kv10.1) (Voltage-gated potassium channel subunit Kv6.3) PROTEIN FAMILY: Potassium channel family, G subfamily, Kv6.3/KCNG3 sub-subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the potassium channel family. G (TC 1.A.1.2) subfamily. Kv6.3/KCNG3 sub-subfamily. FUNCTION: Potassium channel subunit that does not form functional channels by itself. Can form functional heterotetrameric channels with KCNB1; this promotes a reduction in the rate of activation and inactivation of the delayed rectifier voltage-gated potassium channel KCNB1. KEYWORDS: Alternative splicing;Cell membrane;Cytoplasm;Ion channel;Ion transport;Membrane;Potassium;Potassium channel;Potassium transport;Reference proteome;Transmembrane;Transmembrane helix;Transport;Voltage-gated channel SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Cytoplasm Note=Has to be associated with KCNB1 or possibly another partner to get inserted in the plasma membrane. Colocalizes with KCNB1 at the plasma membrane. Remains intracellular in the absence of KCNB1. " Q8TAM2,"PROTEIN NAMES: Tetratricopeptide repeat protein 8 (TPR repeat protein 8) (Bardet-Biedl syndrome 8 protein) ORGANISM: Homo sapiens (Human) FUNCTION: The BBSome complex is thought to function as a coat complex required for sorting of specific membrane proteins to the primary cilia. The BBSome complex is required for ciliogenesis but is dispensable for centriolar satellite function. This ciliogenic function is mediated in part by the Rab8 GDP/GTP exchange factor, which localizes to the basal body and contacts the BBSome. Rab8(GTP) enters the primary cilium and promotes extension of the ciliary membrane. Firstly the BBSome associates with the ciliary membrane and binds to RAB3IP/Rabin8, the guanosyl exchange factor (GEF) for Rab8 and then the Rab8-GTP localizes to the cilium and promotes docking and fusion of carrier vesicles to the base of the ciliary membrane. The BBSome complex, together with the LTZL1, controls SMO ciliary trafficking and contributes to the sonic hedgehog (SHH) pathway regulation. Required for proper BBSome complex assembly and its ciliary localization. KEYWORDS: Alternative splicing;Bardet-Biedl syndrome;Cell membrane;Cell projection;Ciliopathy;Cilium;Cilium biogenesis/degradation;Cytoplasm;Cytoskeleton;Disease variant;Intellectual disability;Membrane;Obesity;Protein transport;Reference proteome;Repeat;Retinitis pigmentosa;TPR repeat;Transport SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Cell projection, cilium membrane Cytoplasm Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriolar satellite Cell projection, cilium MISCELLANEOUS: [Isoform 5]: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay." Q8TAT2,"PROTEIN NAMES: Fibroblast growth factor-binding protein 3 (FGF-BP3) (FGF-binding protein 3) (FGFBP-3) PROTEIN FAMILY: Fibroblast growth factor-binding protein family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the fibroblast growth factor-binding protein family. FUNCTION: Heparin-binding protein which binds to FGF2, prevents binding of FGF2 to heparin and probably inhibits immobilization of FGF2 on extracellular matrix glycosaminoglycans, allowing its release and subsequent activation of FGFR signaling which leads to increased vascular permeability. KEYWORDS: Disulfide bond;Growth factor binding;Heparin-binding;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted " Q8TAU0,PROTEIN NAMES: Homeobox protein Nkx-2.3 (Homeobox protein NK-2 homolog C) PROTEIN FAMILY: NK-2 homeobox family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the NK-2 homeobox family. FUNCTION: Transcription factor. KEYWORDS: DNA-binding;Homeobox;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus Q8TAX9,"PROTEIN NAMES: Gasdermin-B (Gasdermin-like protein) [Cleaved into: Gasdermin-B, N-terminal (GSDMB-NT) (p30); Gasdermin-B, C-terminal (GSDMB-CT) (p16)] PROTEIN FAMILY: Gasdermin family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the gasdermin family. FUNCTION: [Gasdermin-B]: Precursor of a pore-forming protein that acts as a downstream mediator of granzyme-mediated cell death. This form constitutes the precursor of the pore-forming protein: upon cleavage, the released N-terminal moiety (Gasdermin-B, N-terminal) binds to membranes and forms pores, triggering pyroptosis. Also acts as a regulator of epithelial cell repair independently of programmed cell death: translocates to the plasma membrane and promotes epithelial maintenance and repair by regulating PTK2/FAK-mediated phosphorylation of PDGFA.; FUNCTION: [Gasdermin-B, N-terminal]: Pore-forming protein produced by cleavage by granzyme A (GZMA), which causes membrane permeabilization and pyroptosis in target cells of cytotoxic T and natural killer (NK) cells. Key downstream mediator of granzyme-mediated cell death: (1) granzyme A (GZMA), delivered to target cells from cytotoxic T- and NK-cells, (2) specifically cleaves Gasdermin-B to generate this form. After cleavage, moves to the plasma membrane, homooligomerizes within the membrane and forms pores of 10-15 nanometers (nm) of inner diameter, triggering pyroptosis. The different isoforms recognize and bind different phospholipids on membranes, promoting cell death of different target cells.; FUNCTION: [Isoform 4]: Precursor of a pore-forming protein that acts as a downstream mediator of granzyme-mediated cell death and mediates pyroptosis. Following cleavage and activation by granzyme A (GZMA), the N-terminal part binds to membrane inner leaflet lipids, homooligomerizes within the human plasma membrane and forms pores of 10-15 nanometers (nm) of inner diameter, triggering pyroptosis. Recognizes and binds membrane inner leaflet lipids of human cells, such as phosphatidylinositol 4-phosphate, phosphatidylinositol 5-phosphate, bisphosphorylated phosphatidylinositols, such as phosphatidylinositol (4,5)-bisphosphate, and more weakly to phosphatidic acid. Also binds sufatide, a component of the apical membrane of epithelial cells.; FUNCTION: [Isoform 6]: Precursor of a pore-forming protein that acts as a downstream mediator of granzyme-mediated cell death and mediates pyroptosis of human cells. Following cleavage and activation by granzyme A (GZMA), the N-terminal part binds to membrane inner leaflet lipids, homooligomerizes within the human plasma membrane and forms pores of 10-15 nanometers (nm) of inner diameter, triggering pyroptosis.; FUNCTION: [Isoform 1]: Precursor of a pore-forming protein that acts as a downstream mediator of granzyme-mediated cell death and specifically mediates cell death of Gram-negative bacteria in response to infection. Following cleavage and activation by granzyme A (GZMA), the N-terminal part recognizes and binds phospholipids found on Gram-negative bacterial membranes, such as lipid A and cariolipin, homooligomerizes within the bacterial membranes and forms pores, triggering pyroptosis followed by cell death. In contrast to isoform 4, does not bind to membrane inner leaflet lipids of host human cell, such as phosphatidylinositol 4-phosphate, phosphatidylinositol 5-phosphate, bisphosphorylated phosphatidylinositols, such as phosphatidylinositol (4,5)-bisphosphate.; FUNCTION: [Isoform 2]: Not able to trigger pyroptosis.; FUNCTION: [Isoform 3]: Not able to trigger pyroptosis. KEYWORDS: 3D-structure;Alternative splicing;Cell membrane;Coiled coil;Cytolysis;Cytoplasm;Direct protein sequencing;Isopeptide bond;Membrane;Necrosis;Reference proteome;Transmembrane;Transmembrane beta strand;Ubl conjugation SUBCELLULAR LOCATION: [Gasdermin-B]: Cytoplasm Note=Vesicular localization in the apical region of gastric chief cells and colonic surface mucous cells, and the basal region of neuroendocrine cells.; SUBCELLULAR LOCATION: [Gasdermin-B, N-terminal]: Cell membrane ; Multi-pass membrane protein MISCELLANEOUS: Long terminal repeat (LTR) of endogenous retrovirus HERV-H with reverse orientation may serve as alternative promoters of GSDMB gene.; MISCELLANEOUS: [Isoform 1]: Non canonical splice junctions.; MISCELLANEOUS: GSDMB may be used as predictive markers of cervical lymph node metastasis and may help, with a panel of other genes, to discriminate between primary tumors of oral squamous cell carcinoma that metastasize to cervical lymph node and those that do not metastasize." Q8TAZ6,PROTEIN NAMES: CKLF-like MARVEL transmembrane domain-containing protein 2 (Chemokine-like factor superfamily member 2) PROTEIN FAMILY: Chemokine-like factor family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the chemokine-like factor family. KEYWORDS: Alternative splicing;Chemotaxis;Cytokine;Membrane;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. Q8TBC5,PROTEIN NAMES: Zinc finger and SCAN domain-containing protein 18 (Zinc finger protein 447) PROTEIN FAMILY: Krueppel C2H2-type zinc-finger protein family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein family. FUNCTION: May be involved in transcriptional regulation. KEYWORDS: Alternative splicing;DNA-binding;Metal-binding;Nucleus;Reference proteome;Repeat;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Q8TC26,"PROTEIN NAMES: Transmembrane protein 163 PROTEIN FAMILY: TMEM163 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the TMEM163 family. FUNCTION: Zinc ion transporter that mediates zinc efflux and plays a crucial role in intracellular zinc homeostasis. Binds the divalent cations Zn(2+), Ni(2+), and to a minor extent Cu(2+) (By similarity). Is a functional modulator of P2X purinoceptors, including P2RX1, P2RX3, P2RX4 and P2RX7. Plays a role in central nervous system development and is required for myelination, and survival and proliferation of oligodendrocytes. KEYWORDS: Alternative splicing;Cell membrane;Cytoplasmic vesicle;Disease variant;Endosome;Leukodystrophy;Lysosome;Membrane;Phosphoprotein;Reference proteome;Synapse;Transmembrane;Transmembrane helix;Transport;Zinc SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane ; Multi-pass membrane protein Early endosome membrane ; Multi-pass membrane protein Late endosome membrane ; Multi-pass membrane protein Lysosome membrane ; Multi-pass membrane protein Cell membrane ; Multi-pass membrane protein Note=Glutamatergic synaptic vesicles. MISCELLANEOUS: [Isoform 2]: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.; MISCELLANEOUS: [Isoform 3]: Dubious isoform that could be a cloning artifact." Q8TC27,PROTEIN NAMES: Disintegrin and metalloproteinase domain-containing protein 32 (ADAM 32) ORGANISM: Homo sapiens (Human) FUNCTION: May play a role in sperm development and fertilization This is a non-catalytic metalloprotease-like protein. KEYWORDS: Direct protein sequencing;Disulfide bond;EGF-like domain;Glycoprotein;Membrane;Phosphoprotein;Reference proteome;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Single-pass type I membrane protein Q8TC41,"PROTEIN NAMES: E3 ubiquitin-protein ligase RNF217 (IBR domain-containing protein 1) (Opposite STL) (RING finger protein 217) PROTEIN FAMILY: RBR family, RNF217 subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the RBR family. RNF217 subfamily. FUNCTION: E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates the degradation of the iron exporter ferroportin/SLC40A1 and thus regulates iron homeostasis. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Alternative splicing;Cytoplasm;Membrane;Metal-binding;Reference proteome;Repeat;Transferase;Transmembrane;Transmembrane helix;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Membrane ; Single-pass membrane protein Cytoplasm " Q8TCD5,"PROTEIN NAMES: 5'(3')-deoxyribonucleotidase, cytosolic type (Cytosolic 5',3'-pyrimidine nucleotidase) (Deoxy-5'-nucleotidase 1) (dNT-1) PROTEIN FAMILY: 5'(3')-deoxyribonucleotidase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the 5'(3')-deoxyribonucleotidase family. FUNCTION: Dephosphorylates the 5' and 2'(3')-phosphates of deoxyribonucleotides, with a preference for dUMP and dTMP, intermediate activity towards dGMP, and low activity towards dCMP and dAMP. KEYWORDS: 3D-structure;Alternative splicing;Cytoplasm;Direct protein sequencing;Hydrolase;Magnesium;Metal-binding;Nucleotide metabolism;Nucleotide-binding;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cytoplasm. MISCELLANEOUS: [Isoform 2]: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay." Q8TCJ0,"PROTEIN NAMES: F-box only protein 25 ORGANISM: Homo sapiens (Human) FUNCTION: Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. May play a role in accumulation of expanded polyglutamine (polyQ) protein huntingtin (HTT) (By similarity). PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Actin-binding;Alternative splicing;Chromosomal rearrangement;Direct protein sequencing;Nucleus;Reference proteome;Ubl conjugation pathway SUBCELLULAR LOCATION: Nucleus Note=In the nucleus, associates with a subnuclear dot-like structure. Colocalized with SKP1. " Q8TCT0,"PROTEIN NAMES: Ceramide kinase (hCERK) (Acylsphingosine kinase) (Lipid kinase 4) (LK4) ORGANISM: Homo sapiens (Human) FUNCTION: Catalyzes specifically the phosphorylation of ceramide to form ceramide 1-phosphate. Acts efficiently on natural and analog ceramides (C6, C8, C16 ceramides, and C8-dihydroceramide), to a lesser extent on C2-ceramide and C6-dihydroceramide, but not on other lipids, such as various sphingosines. Shows a greater preference for D-erythro isomer of ceramides. Binds phosphoinositides. KEYWORDS: Alternative splicing;ATP-binding;Calcium;Cell membrane;Cytoplasm;Kinase;Lipid metabolism;Magnesium;Membrane;Nucleotide-binding;Phosphoprotein;Reference proteome;Transferase SUBCELLULAR LOCATION: Cytoplasm Cell membrane ; Peripheral membrane protein " Q8TCX5,"PROTEIN NAMES: Rhophilin-1 (GTP-Rho-binding protein 1) PROTEIN FAMILY: RHPN family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the RHPN family. FUNCTION: Has no enzymatic activity. May serve as a target for Rho, and interact with some cytoskeletal component upon Rho binding or relay a Rho signal to other molecules. KEYWORDS: Coiled coil;Phosphoprotein;Reference proteome " Q8TD17,PROTEIN NAMES: Zinc finger protein 398 (Zinc finger DNA-binding protein p52/p71) PROTEIN FAMILY: Krueppel C2H2-type zinc-finger protein family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein family. FUNCTION: Functions as a transcriptional activator. KEYWORDS: Activator;Alternative splicing;DNA-binding;Isopeptide bond;Metal-binding;Nucleus;Reference proteome;Repeat;Transcription;Transcription regulation;Ubl conjugation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Q8TDB8,"PROTEIN NAMES: Solute carrier family 2, facilitated glucose transporter member 14 (Glucose transporter type 14) (GLUT-14) PROTEIN FAMILY: Major facilitator superfamily, Sugar transporter family, Glucose transporter subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily. FUNCTION: Hexose transporter that can mediate the transport of glucose and dehydroascorbate across the cell membrane. KEYWORDS: Alternative splicing;Cell membrane;Developmental protein;Differentiation;Glycoprotein;Membrane;Reference proteome;Spermatogenesis;Sugar transport;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein MISCELLANEOUS: GLUT14 is a recent (less than 5 M year old) duplication of GLUT3." Q8TDD1,"PROTEIN NAMES: ATP-dependent RNA helicase DDX54 (ATP-dependent RNA helicase DP97) (DEAD box RNA helicase 97 kDa) (DEAD box protein 54) PROTEIN FAMILY: DEAD box helicase family, DDX54/DBP10 subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the DEAD box helicase family. DDX54/DBP10 subfamily. FUNCTION: Has RNA-dependent ATPase activity. Represses the transcriptional activity of nuclear receptors. KEYWORDS: 3D-structure;Alternative splicing;ATP-binding;Helicase;Hydrolase;Nucleotide-binding;Nucleus;Phosphoprotein;Reference proteome;RNA-binding;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus, nucleolus " Q8TDL5,"PROTEIN NAMES: BPI fold-containing family B member 1 (Long palate, lung and nasal epithelium carcinoma-associated protein 1) (von Ebner minor salivary gland protein) (VEMSGP) PROTEIN FAMILY: BPI/LBP/Plunc superfamily, Plunc family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the BPI/LBP/Plunc superfamily. Plunc family. FUNCTION: May play a role in innate immunity in mouth, nose and lungs. Binds bacterial lipopolysaccharide (LPS) and modulates the cellular responses to LPS. KEYWORDS: Alternative splicing;Disulfide bond;Glycoprotein;Immunity;Innate immunity;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted " Q8TE04,"PROTEIN NAMES: Pantothenate kinase 1 (hPanK) (hPanK1) (Pantothenic acid kinase 1) PROTEIN FAMILY: Type II pantothenate kinase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the type II pantothenate kinase family. FUNCTION: [Isoform 1]: Catalyzes the phosphorylation of pantothenate to generate 4'-phosphopantothenate in the first and rate-determining step of coenzyme A (CoA) synthesis. PATHWAY: Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 1/5. KEYWORDS: 3D-structure;Alternative splicing;ATP-binding;Coenzyme A biosynthesis;Cytoplasm;Cytoplasmic vesicle;Endosome;Kinase;Nucleotide-binding;Nucleus;Phosphoprotein;Reference proteome;Transferase SUBCELLULAR LOCATION: Cytoplasm.; SUBCELLULAR LOCATION: [Isoform 1]: Nucleus Nucleus, nucleolus.; SUBCELLULAR LOCATION: [Isoform 2]: Cytoplasm, cytosol Cytoplasmic vesicle, clathrin-coated vesicle Recycling endosome " Q8TE68,PROTEIN NAMES: Epidermal growth factor receptor kinase substrate 8-like protein 1 (EPS8-like protein 1) (Epidermal growth factor receptor pathway substrate 8-related protein 1) (EPS8-related protein 1) PROTEIN FAMILY: EPS8 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the EPS8 family. FUNCTION: Stimulates guanine exchange activity of SOS1. May play a role in membrane ruffling and remodeling of the actin cytoskeleton. KEYWORDS: 3D-structure;Alternative splicing;Coiled coil;Cytoplasm;Phosphoprotein;Reference proteome;SH3 domain SUBCELLULAR LOCATION: Cytoplasm Q8TE77,"PROTEIN NAMES: Protein phosphatase Slingshot homolog 3 (SSH-like protein 3) (SSH-3L) (hSSH-3L) PROTEIN FAMILY: Protein-tyrosine phosphatase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the protein-tyrosine phosphatase family. FUNCTION: Protein phosphatase which may play a role in the regulation of actin filament dynamics. Can dephosphorylate and activate the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and stimulates their disassembly (By similarity). KEYWORDS: Acetylation;Alternative splicing;Cytoplasm;Cytoskeleton;Hydrolase;Nucleus;Phosphoprotein;Protein phosphatase;Reference proteome SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton Nucleus MISCELLANEOUS: Tyrosine phosphatase activity has not been demonstrated for this protein to date." Q8TEY7,"PROTEIN NAMES: Ubiquitin carboxyl-terminal hydrolase 33 (Deubiquitinating enzyme 33) (Ubiquitin thioesterase 33) (Ubiquitin-specific-processing protease 33) (VHL-interacting deubiquitinating enzyme 1) (hVDU1) PROTEIN FAMILY: Peptidase C19 family, USP20/USP33 subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the peptidase C19 family. USP20/USP33 subfamily. FUNCTION: Deubiquitinating enzyme involved in various processes such as centrosome duplication, cellular migration and beta-2 adrenergic receptor/ADRB2 recycling. Involved in regulation of centrosome duplication by mediating deubiquitination of CCP110 in S and G2/M phase, leading to stabilize CCP110 during the period which centrioles duplicate and elongate. Involved in cell migration via its interaction with intracellular domain of ROBO1, leading to regulate the Slit signaling. Plays a role in commissural axon guidance cross the ventral midline of the neural tube in a Slit-dependent manner, possibly by mediating the deubiquitination of ROBO1. Acts as a regulator of G-protein coupled receptor (GPCR) signaling by mediating the deubiquitination of beta-arrestins (ARRB1 and ARRB2) and beta-2 adrenergic receptor (ADRB2). Plays a central role in ADRB2 recycling and resensitization after prolonged agonist stimulation by constitutively binding ADRB2, mediating deubiquitination of ADRB2 and inhibiting lysosomal trafficking of ADRB2. Upon dissociation, it is probably transferred to the translocated beta-arrestins, leading to beta-arrestins deubiquitination and disengagement from ADRB2. This suggests the existence of a dynamic exchange between the ADRB2 and beta-arrestins. Deubiquitinates DIO2, thereby regulating thyroid hormone regulation. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. KEYWORDS: 3D-structure;Alternative splicing;Cytoplasm;Cytoskeleton;Endocytosis;Golgi apparatus;Hydrolase;Metal-binding;Phosphoprotein;Protease;Reference proteome;Repeat;Thiol protease;Ubl conjugation;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cytoplasm, perinuclear region Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Note=Associates with centrosomes predominantly in S and G2 phases but less in G1 phase.; SUBCELLULAR LOCATION: [Isoform 3]: Golgi apparatus " Q8TL28,"PROTEIN NAMES: Ulilysin PROTEIN FAMILY: Peptidase M43B family ORGANISM: Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A) SIMILARITY: Belongs to the peptidase M43B family. FUNCTION: Metalloprotease which in vitro specifically cleaves IGFBP-2 to -6, insulin, and extracellular matrix proteins but not IGFBP-1 or IGF-II. Shows a preference for substrates with an arginine in the P1' position, the first position downstream of the scissile bond. KEYWORDS: 3D-structure;Calcium;Direct protein sequencing;Disulfide bond;Hydrolase;Metal-binding;Metalloprotease;Protease;Reference proteome;Signal;Zinc;Zymogen " Q8TXY4,"PROTEIN NAMES: 5,10-methylenetetrahydromethanopterin reductase (Coenzyme F420-dependent N(5),N(10)-methylenetetrahydromethanopterin reductase) (Methylene-H(4)MPT reductase) PROTEIN FAMILY: Mer family ORGANISM: Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938) SIMILARITY: Belongs to the mer family. FUNCTION: Catalyzes the reversible reduction of methylene-H(4)MPT to methyl-H(4)MPT. PATHWAY: One-carbon metabolism; methanogenesis from CO(2); methyl-coenzyme M from 5,10-methylene-5,6,7,8-tetrahydromethanopterin: step 1/2. KEYWORDS: 3D-structure;Cytoplasm;Direct protein sequencing;Methanogenesis;One-carbon metabolism;Oxidoreductase;Reference proteome SUBCELLULAR LOCATION: Cytoplasm. " Q8TZE8,"PROTEIN NAMES: L-asparaginase PROTEIN FAMILY: Asparaginase 1 family ORGANISM: Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) SIMILARITY: Belongs to the asparaginase 1 family. FUNCTION: Catalyzes the hydrolysis of L-asparagine into L-aspartate and ammonia. Displays no glutaminase activity, a highly desirable therapeutic property. KEYWORDS: 3D-structure;Hydrolase;Reference proteome MISCELLANEOUS: Microbial L-asparaginase is considered as an important biopharmaceutical drug enzyme in the treatment of childhood acute lymphoblastic leukemia (ALL). It functions by reducing the availability of circulatory L-asparagine to tumor cells. The principle behind the cytotoxic effect of L-asparaginase stems from the fact that the leukemic lymphoblastic tumor cells and other blood tumor cells are auxotrophs towards the L-asparagine and exhibit low L-asparagine synthetase (ASNS) activity for de novo synthesis of L-asparagine. Therefore, these tumor cells are required for exogenous supply of L-asparagine for proliferation and survival. L-Asparaginase has also been used for making a diagnostic biosensor as the amount of ammonia produced by the action of the enzyme directly correlates to the level of L-asparagine in a patient's blood (Probable). P.furiosus L-asparaginase and its mutants show significant killing of cultured human leukemic cell lines HL60, MCF-7, and K562." Q8U0Z6,PROTEIN NAMES: Membrane-bound hydrogenase subunit alpha (MBH-alpha) PROTEIN FAMILY: Complex I 49 kDa subunit family ORGANISM: Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) SIMILARITY: Belongs to the complex I 49 kDa subunit family. FUNCTION: Alpha subunit of a hydrogen-evolving hydrogenase that utilizes protons both as a substrate for hydrogen production and proton translocation. Acts by coupling the redox reaction via ferredoxin and iron-sulfur (Fe-S) clusters to proton translocation across the membrane thereby conserving the redox energy in a proton gradient. KEYWORDS: 3D-structure;Cell membrane;Direct protein sequencing;Membrane;Metal-binding;Nickel;Oxidoreductase;Reference proteome SUBCELLULAR LOCATION: Cell membrane Q8U1N9,"PROTEIN NAMES: DNA double-strand break repair protein Mre11 (pfMre11) PROTEIN FAMILY: MRE11/RAD32 family ORGANISM: Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) SIMILARITY: Belongs to the MRE11/RAD32 family. FUNCTION: Part of the Rad50/Mre11 complex, which is involved in the early steps of DNA double-strand break (DSB) repair. The complex may facilitate opening of the processed DNA ends to aid in the recruitment of HerA and NurA. Mre11 binds to DSB ends and has both double-stranded 3'-5' exonuclease activity and single-stranded endonuclease activity. KEYWORDS: 3D-structure;DNA damage;DNA repair;Endonuclease;Exonuclease;Hydrolase;Manganese;Metal-binding;Nuclease;Reference proteome " Q8U1Y4,"PROTEIN NAMES: Desampylase (JAMM/MPN(+) metalloprotease) (PfJAMM1) PROTEIN FAMILY: Peptidase M67B family ORGANISM: Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) SIMILARITY: Belongs to the peptidase M67B family. FUNCTION: Metalloprotease that displays desampylase (DSAMP) activity, cleaving ubiquitin-like small archaeal modifier proteins (SAMP1, SAMP2 and SAMP3) from protein conjugates (isopeptide- and linear-linked). Thus, likely regulates sampylation and the pools of 'free' SAMP available for protein modification. In vitro, is also able to cleave non-physiological ubiquitin (Ub) substrates, such as 'Met1-', 'Lys48-', and 'Lys63'-linked Ub dimers (Ub2), and to remove Ub tags from diverse proteins. KEYWORDS: 3D-structure;Disulfide bond;Hydrolase;Metal-binding;Metalloprotease;Protease;Reference proteome;Zinc " Q8VBU8,"PROTEIN NAMES: Protein BANP (Btg3-associated nuclear protein) (Scaffold/matrix-associated region-1-binding protein) PROTEIN FAMILY: BANP/SMAR1 family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the BANP/SMAR1 family. FUNCTION: Controls V(D)J recombination during T-cell development by repressing T-cell receptor (TCR) beta enhancer function. Binds to scaffold/matrix attachment region beta (S/MARbeta), an ATC-rich DNA sequence located upstream of the TCR beta enhancer. Represses cyclin D1 transcription by recruiting HDAC1 to its promoter, thereby diminishing H3K9ac, H3S10ph and H4K8ac levels. Promotes TP53 activation, which causes cell cycle arrest and inhibits tumor growth. KEYWORDS: Acetylation;Alternative splicing;Cell cycle;Chromatin regulator;Coiled coil;Developmental protein;DNA-binding;Isopeptide bond;Nucleus;Phosphoprotein;Reference proteome;Repressor;Transcription;Transcription regulation;Ubl conjugation SUBCELLULAR LOCATION: Nucleus " Q8VC66,"PROTEIN NAMES: Afadin- and alpha-actinin-binding protein (ADIP) (Afadin DIL domain-interacting protein) PROTEIN FAMILY: ADIP family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the ADIP family. FUNCTION: Belongs to an adhesion system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs). May connect the nectin-afadin and E-cadherin-catenin system through alpha-actinin and may be involved in organization of the actin cytoskeleton at AJs through afadin and alpha-actinin. Acts as a centrosome maturation factor, probably by maintaining the integrity of the pericentriolar material and proper microtubule nucleation at mitotic spindle poles. The function seems to implicate at least in part WRAP73; the SSX2IP:WRAP73 complex is proposed to act as regulator of spindle anchoring at the mitotic centrosome (By similarity). Involved in cell movement: localizes at the leading edge of moving cells in response to PDGF and is required for the formation of the leading edge and the promotion of cell movement, possibly via activation of Rac signaling. Involved in ciliogenesis (By similarity). It is required for targeted recruitment of the BBSome, CEP290, RAB8, and SSTR3 to the cilia (By similarity). KEYWORDS: Cell adhesion;Cell junction;Cell projection;Cilium biogenesis/degradation;Coiled coil;Cytoplasm;Cytoskeleton;Nucleus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cell junction, adherens junction Nucleus Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriolar satellite Cytoplasm, cytoskeleton, cilium basal body Note=Not found at cell-matrix AJs. " Q8VCC1,"PROTEIN NAMES: 15-hydroxyprostaglandin dehydrogenase [NAD(+)] (15-PGDH) (Eicosanoid/docosanoid dehydrogenase [NAD(+)]) (Prostaglandin dehydrogenase 1) PROTEIN FAMILY: Short-chain dehydrogenases/reductases (SDR) family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FUNCTION: Catalyzes the NAD-dependent dehydrogenation (oxidation) of a broad array of hydroxylated polyunsaturated fatty acids (mainly eicosanoids and docosanoids, including prostaglandins, lipoxins and resolvins), yielding their corresponding keto (oxo) metabolites (By similarity). Decreases the levels of the pro-proliferative prostaglandins such as prostaglandin E2 (whose activity is increased in cancer because of an increase in the expression of cyclooxygenase 2) and generates oxo-fatty acid products that can profoundly influence cell function by abrogating pro-inflammatory cytokine expression. Converts resolvins E1, D1 and D2 to their oxo products, which represents a mode of resolvin inactivation. Resolvin E1 plays important roles during the resolution phase of acute inflammation, while resolvins D1 and D2 have a unique role in obesity-induced adipose inflammation (By similarity). KEYWORDS: Cytoplasm;Fatty acid metabolism;Lipid metabolism;NAD;Oxidoreductase;Prostaglandin metabolism;Reference proteome;Tumor suppressor SUBCELLULAR LOCATION: Cytoplasm " Q8VCL5,"PROTEIN NAMES: Voltage-gated purine nucleotide uniporter SLC17A9 (Solute carrier family 17 member 9) (Vesicular nucleotide transporter) (VNUT) PROTEIN FAMILY: Major facilitator superfamily, Sodium/anion cotransporter family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the major facilitator superfamily. Sodium/anion cotransporter family. FUNCTION: Voltage-gated ATP nucleotide uniporter that can also transport the purine nucleotides ADP and GTP. Uses the membrane potential as the driving force to control ATP accumulation in lysosomes and secretory vesicles. By controlling ATP storage in lysosomes, regulates ATP-dependent proteins of these organelles. Also indirectly regulates the exocytosis of ATP through its import into lysosomes in astrocytes and secretory vesicles such as adrenal chromaffin granules, mucin granules and synaptic vesicles (By similarity). KEYWORDS: Cytoplasmic vesicle;Lysosome;Membrane;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, chromaffin granule membrane ; Multi-pass membrane protein Cytoplasmic vesicle, secretory vesicle membrane ; Multi-pass membrane protein Lysosome membrane ; Multi-pass membrane protein Note=Localizes to mucin granules and vesicles. " Q8VD52,"PROTEIN NAMES: Chronophin (Pyridoxal phosphate phosphatase) (PLP phosphatase) (Reg I-binding protein 1) PROTEIN FAMILY: HAD-like hydrolase superfamily ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the HAD-like hydrolase superfamily. FUNCTION: Functions as a pyridoxal phosphate (PLP) phosphatase, which also catalyzes the dephosphorylation of pyridoxine 5'-phosphate (PNP) and pyridoxamine 5'-phosphate (PMP), with order of substrate preference PLP > PNP > PMP and therefore plays a role in vitamin B6 metabolism. Also functions as a protein serine phosphatase that specifically dephosphorylates 'Ser-3' in proteins of the actin-depolymerizing factor (ADF)/cofilin family like CFL1 and DSTN. Thereby, regulates cofilin-dependent actin cytoskeleton reorganization, being required for normal progress through mitosis and normal cytokinesis. Does not dephosphorylate phosphothreonines in LIMK1. Does not dephosphorylate peptides containing phosphotyrosine. KEYWORDS: Cell membrane;Cell projection;Cytoplasm;Cytoskeleton;Direct protein sequencing;Hydrolase;Magnesium;Membrane;Metal-binding;Pyridoxal phosphate;Reference proteome SUBCELLULAR LOCATION: Cytoplasm, cytosol Cytoplasm, cytoskeleton Cell projection, ruffle membrane ; Peripheral membrane protein ; Cytoplasmic side Cell projection, lamellipodium membrane ; Peripheral membrane protein ; Cytoplasmic side Cell membrane ; Peripheral membrane protein ; Cytoplasmic side Note=Colocalizes with the actin cytoskeleton in membrane ruffles and lamellipodia. Diffusely distributed throughout the cytosol during pro-metaphase and metaphase. Detected at the dynamic cell poles during telophase. Detected at the cleavage furrow and contractile ring during cytokinesis. Transiently detected at the plasma membrane in late stages of cytokinesis. Detected at the midbody. " Q8VEE1,"PROTEIN NAMES: LIM and cysteine-rich domains protein 1 ORGANISM: Mus musculus (Mouse) FUNCTION: Transcriptional cofactor that restricts GATA6 function by inhibiting DNA-binding, resulting in repression of GATA6 transcriptional activation of downstream target genes. Represses GATA6-mediated trans activation of lung- and cardiac tissue-specific promoters. Inhibits DNA-binding by GATA4 and GATA1 to the cTNC promoter. Plays a critical role in the development of cardiac hypertrophy via activation of calcineurin/nuclear factor of activated T-cells signaling pathway. KEYWORDS: Cytoplasm;LIM domain;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Repeat;Repressor;Transcription;Transcription regulation;Zinc SUBCELLULAR LOCATION: Cytoplasm Nucleus Note=May shuttle between the cytoplasm and the nucleus. " Q8VHK5,"PROTEIN NAMES: Membrane protein MLC1 ORGANISM: Mus musculus (Mouse) FUNCTION: Transmembrane protein mainly expressed in brain atrocytes that may play a role in transport across the blood-brain and brain-cerebrospinal fluid barriers. Regulates the response of astrocytes to hypo-osmosis by promoting calcium influx. May function as regulatory protein of membrane protein complexes such as ion channels. KEYWORDS: Cell membrane;Cytoplasm;Endoplasmic reticulum;Membrane;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Multi-pass membrane protein Cell membrane ; Multi-pass membrane protein Cytoplasm, perinuclear region Endoplasmic reticulum " Q8VHQ3,"PROTEIN NAMES: Protein phosphatase 1 regulatory inhibitor subunit 16B (CAAX box protein TIMAP) (TGF-beta-inhibited membrane-associated protein) ORGANISM: Mus musculus (Mouse) FUNCTION: Regulator of protein phosphatase 1 (PP1) that acts as a positive regulator of pulmonary endothelial cell (EC) barrier function. Protects the endothelial barrier from lipopolysaccharide (LPS)-induced vascular leakage. Involved in the regulation of the PI3K/AKT signaling pathway (By similarity). Involved in the regulation of angiogenesis and endothelial cell proliferation through the control of ECE1 dephosphorylation, trafficking and activity (By similarity). Involved in the regulation of endothelial cell filopodia extension (By similarity). May be a downstream target for TGF-beta1 signaling cascade in endothelial cells (By similarity). Involved in PKA-mediated moesin dephosphorylation which is important in EC barrier protection against thrombin stimulation. Promotes the interaction of PPP1CA with RPSA/LAMR1 and in turn facilitates the dephosphorylation of RPSA/LAMR1 (By similarity). Involved in the dephosphorylation of EEF1A1 (By similarity). KEYWORDS: ANK repeat;Cell membrane;Cell projection;Coiled coil;Lipoprotein;Membrane;Methylation;Nucleus;Palmitate;Phosphoprotein;Prenylation;Reference proteome;Repeat SUBCELLULAR LOCATION: Cell membrane Cell membrane ; Lipid-anchor Nucleus Cell projection Note=Colocalizes with RPSA/LAMR1 in the cell membrane (By similarity). Localizes to the perinuclear region (By similarity). Colocalizes with PTEN at the tip of EC projections (By similarity). " Q8VIF2,"PROTEIN NAMES: Serine protease 42 (Testis serine protease 2) PROTEIN FAMILY: Peptidase S1 family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the peptidase S1 family. FUNCTION: Plays a role in spermatogenesis. Involved in germ cell survival during meiosis. Lacks protease activity in vitro. KEYWORDS: Cell membrane;Cytoplasm;Differentiation;Disulfide bond;Glycoprotein;GPI-anchor;Hydrolase;Lipoprotein;Membrane;Protease;Reference proteome;Serine protease;Signal;Spermatogenesis SUBCELLULAR LOCATION: Cytoplasm Cell membrane ; Lipid-anchor, GPI-anchor " Q8VIH3,"PROTEIN NAMES: Solute carrier family 28 member 3 (Concentrative Na(+)-nucleoside cotransporter 3) (CNT 3) (rCNT3) PROTEIN FAMILY: Concentrative nucleoside transporter (CNT) family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the concentrative nucleoside transporter (CNT) (TC 2.A.41) family. FUNCTION: Sodium-dependent, pyrimidine- and purine-selective. Involved in the homeostasis of endogenous nucleosides. Exhibits the transport characteristics of the nucleoside transport system cib or N3 subtype (N3/cib) (with marked transport of both thymidine and inosine). Employs a 2:1 sodium/nucleoside ratio. Also able to transport gemcitabine, 3'-azido-3'-deoxythymidine (AZT), ribavirin and 3-deazauridine. KEYWORDS: Cell membrane;Membrane;Reference proteome;Symport;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein " Q8VWF1,"PROTEIN NAMES: SH3 domain-containing protein 2 (AtSH3P2) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Regulator for autophaosome formation and/or maturation. Binds phosphatidylinositol-phosphate; highest affinity for vesicles containing PtdIns(3,4,5)P(3), followed by those containing PtdIns(4,5)P(2) and PtdIns(3,4)P(2), with minimal binding to phosphatidylinositol monophosphates, including PtdIns(3)P. Together with DRP1A, converts the fused vesicles to tubular structures at the cell plate during cytokinesis. KEYWORDS: Alternative splicing;Cell membrane;Coiled coil;Cytoplasm;Cytoplasmic vesicle;Endosome;Membrane;Reference proteome;SH3 domain SUBCELLULAR LOCATION: Cytoplasm Cytoplasmic vesicle, clathrin-coated vesicle Cell membrane Late endosome Cytoplasmic vesicle, autophagosome membrane ; Peripheral membrane protein Note=Transolcate from the cytosol to the phagophore assembly site/preautophagosome structure upon autophagy induction. Localized at the leading edge of the cell plate in dividing cells, especially in constricted or curved regions. Observed at the plasma membrane and in endosomal compartments in non-dividing cells. MISCELLANEOUS: Knockdown of SH3P2 is developmentally lethal and significantly suppresses autophagosome formation." Q8VWG7,"PROTEIN NAMES: TPR repeat-containing thioredoxin TDX (HSP70-interacting protein 2) (AtHIP2) (Tetratricoredoxin) (AtTDX) PROTEIN FAMILY: Thioredoxin family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the thioredoxin family. FUNCTION: Thiol-disulfide oxidoreductase that possesses insulin disulfide bonds reducing activity, disulfide reductase, foldase chaperone and holdase chaperone activities. Heat shock causes oligomerization and formation of high molecular weiht (HMW) complexes with concomitant functional switching from a disulfide reductase and foldase chaperone to a holdase chaperone. May interact with HSP70 proteins through the TPR repeats. KEYWORDS: Acetylation;Alternative splicing;Chaperone;Disulfide bond;Electron transport;Redox-active center;Reference proteome;Repeat;TPR repeat;Transport " Q8VWK0,PROTEIN NAMES: 26S proteasome non-ATPase regulatory subunit 12 homolog B (26S proteasome regulatory subunit RPN5b) (AtRPN5b) (26S proteasome regulatory subunit p55 homolog B) PROTEIN FAMILY: Proteasome subunit p55 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the proteasome subunit p55 family. FUNCTION: Acts as a regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins (By similarity). Acts redundantly with RPN5A. KEYWORDS: Alternative splicing;Coiled coil;Cytoplasm;Nucleus;Proteasome;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Nucleus Q8VY08,"PROTEIN NAMES: Leucine carboxyl methyltransferase 1 homolog (Protein SUPPRESSOR OF BRI1) ([Phosphatase 2A protein]-leucine-carboxy methyltransferase 1) PROTEIN FAMILY: Methyltransferase superfamily, LCMT family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the methyltransferase superfamily. LCMT family. FUNCTION: Methylates the carboxyl group of the C-terminal leucine residue of protein phosphatase 2A (PP2A) catalytic subunits to form alpha-leucine ester residues (Probable). Involved in brassinosteroid (BR) signaling. Plays a negative role in BR signaling pathway. Functions as a positive regulator of BRI1 receptor-kinase degradation. Methylates PP2A, thus facilitating its association with activated BRI1. This leads to receptor dephosphorylation and degradation, and thus to the termination of BR signaling. May act upstream of ASK7/BIN2. Involved in methylation of PP2A during environmental stress responses. KEYWORDS: Cytoplasm;Membrane;Methyltransferase;Reference proteome;S-adenosyl-L-methionine;Stress response;Transferase SUBCELLULAR LOCATION: Cytoplasm Membrane ; Peripheral membrane protein. " Q8VY26,"PROTEIN NAMES: Carotenoid cleavage dioxygenase 8, chloroplastic (AtCCD8) (AtNCED8) (Protein MORE AXILLARY BRANCHING 4) (Protein MORE AXILLARY GROWTH 4) PROTEIN FAMILY: Carotenoid oxygenase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the carotenoid oxygenase family. FUNCTION: Involved in strigolactones biosynthesis by cleaving the C(27) 9-cis-10'-apo-beta-carotenal produced by CCD7. Produces the C(19) carlactone and a C(8) hydroxyaldehyde. Also shows lower activity with all-trans-10'-apo-beta-carotenal producing a C(9) dialdehyde and the C(18) 13-apo-beta-carotenone. Strigolactones are hormones that inhibit tillering and shoot branching through the MAX-dependent pathway, contribute to the regulation of shoot architectural response to phosphate-limiting conditions and function as rhizosphere signal that stimulates hyphal branching of arbuscular mycorrhizal fungi and trigger seed germination of root parasitic weeds. Also active on other carotenoid substrates like licopene or zeaxanthin. KEYWORDS: Chloroplast;Dioxygenase;Iron;Metal-binding;Oxidoreductase;Plastid;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast MISCELLANEOUS: The branching phenotypes of the max1, ccd7/max3 and ccd8/max4 mutants can be rescued by exogenous treatment with the synthetic strigolactone analogs GR24 and 4BD (Probable). The ccd8/max4 mutant responds also to carlactone (CL) and methyl carlactonoate (MeCLA), but not the lbo ccd8/max4 double mutant (Probable)." Q8VY27,"PROTEIN NAMES: Exocyst complex component EXO70H1 (AtExo70h1) (Exocyst subunit Exo70 family protein H1) PROTEIN FAMILY: EXO70 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the EXO70 family. FUNCTION: Component of an exocyst subcomplex specifically involved in autophagy-related, Golgi-independent membrane traffic to the vacuole. Regulates autophagosome formation and autophagy-related Golgi-independent import into the vacuole (By similarity). Involved in defense responses to pathogenic bacteria (e.g. P.syringae pv. maculicola). KEYWORDS: Cytoplasm;Cytoplasmic vesicle;Membrane;Nucleus;Plant defense;Reference proteome;Transport SUBCELLULAR LOCATION: Cytoplasmic vesicle, phagosome Cytoplasm Nucleus Endomembrane system Note=Localized in the vesicle-like structures in a peri-nuclear and nuclear pattern. " Q8VYC8,"PROTEIN NAMES: E3 ubiquitin protein ligase RIN2 (AMF receptor-like protein 1A) (RING-type E3 ubiquitin transferase RIN2) (RPM1-interacting protein 2) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: E3 ubiquitin protein ligase that acts as a positive regulator of RPM1- and RPS2-dependent hypersensitive response (HR), in association with RIN3. Probably not required for RPM1 degradation during HR. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Hypersensitive response;Membrane;Metal-binding;Plant defense;Reference proteome;Transferase;Transmembrane;Transmembrane helix;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Membrane ; Multi-pass membrane protein " Q8VYP5,"PROTEIN NAMES: Probable protein S-acyltransferase 14 (Probable palmitoyltransferase At3g60800) (Zinc finger DHHC domain-containing protein At3g60800) PROTEIN FAMILY: DHHC palmitoyltransferase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the DHHC palmitoyltransferase family. FUNCTION: Palmitoyl acyltransferase. KEYWORDS: Acyltransferase;Golgi apparatus;Lipoprotein;Membrane;Palmitate;Reference proteome;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus, trans-Golgi network membrane ; Multi-pass membrane protein " Q8VYY5,"PROTEIN NAMES: Receptor-like serine/threonine-protein kinase NCRK PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. KEYWORDS: ATP-binding;Cell membrane;Endosome;Glycoprotein;Kinase;Membrane;Nucleotide-binding;Phosphoprotein;Receptor;Reference proteome;Serine/threonine-protein kinase;Signal;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein Prevacuolar compartment membrane Endosome " Q8VZ20,"PROTEIN NAMES: Trihelix transcription factor ASR3 (Protein ARABIDOPSIS SH4-RELATED3) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Transcriptional repressor that binds DNA and plays a negative role in regulating microbe-associated molecular patterns-(MAMPs, e.g. flg22, elf18, chitin, and LPS) triggered immunity (PTI) by negatively regulating immune gene expression. KEYWORDS: Alternative splicing;DNA-binding;Nucleus;Phosphoprotein;Plant defense;Reference proteome;Repeat;Repressor;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " Q8VZ80,"PROTEIN NAMES: Polyol transporter 5 (Protein POLYOL TRANSPORTER 5) (AtPLT5) (Sugar-proton symporter PLT5) PROTEIN FAMILY: Major facilitator superfamily, Sugar transporter family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. FUNCTION: Plasma membrane broad-spectrum sugar-proton symporter. Mediates the uptake of linear polyols such as sorbitol, xylitol, erythritol or glycerol. Can transport the cyclic polyol myo-inositol and different hexoses, pentoses (including ribose), tetroses and sugar alcohols. KEYWORDS: Cell membrane;Membrane;Reference proteome;Sugar transport;Symport;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein " Q8VZA0,"PROTEIN NAMES: Protein SIEL (Protein SHORT-ROOT INTERACTING EMBRYONIC LETHAL) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Intracellular shuttle that promotes movement of SHR from the stele into the endodermis. Required for SHR association to endosomes and localization, and for intercellular movement of SHR. KEYWORDS: Cytoplasm;Endosome;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus Endosome Cytoplasm, cell cortex Note=Intact microtubules promote the localization to endosomes and the cell cortex. MISCELLANEOUS: Hypomorphs (siel-3 and siel-4) have reduced movement of SHR and cause defects in root patterning. Inhibition of microtubules results in mis-localization of SIEL." Q8VZA5,"PROTEIN NAMES: Probable 3-deoxy-D-manno-octulosonic acid transferase, mitochondrial (AtKdtA) (Kdo transferase A) (Bifunctional Kdo transferase) (Kdo-lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase) (Lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase) PROTEIN FAMILY: Glycosyltransferase group 1 family, Glycosyltransferase 30 subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 30 subfamily. FUNCTION: Involved in the biosynthesis of lipid A, a phosphorylated glycolipid that in bacteria anchors the lipopolysaccharide to the outer membrane of the cell. Catalyzes the transfer of two 3-deoxy-D-manno-octulosonate (Kdo) residues from CMP-Kdo to lipid IV(A), the tetraacyldisaccharide-1,4'-bisphosphate precursor of lipid A. Lipid A-like molecules in plants may serve as structural components of the outer membranes of mitochondria and/or chloroplasts, or may be involved in signal transduction or plant defense responses. PATHWAY: Glycolipid biosynthesis; KDO(2)-lipid A biosynthesis; KDO(2)-lipid A from CMP-3-deoxy-D-manno-octulosonate and lipid IV(A): step 1/4.; PATHWAY: Glycolipid biosynthesis; KDO(2)-lipid A biosynthesis; KDO(2)-lipid A from CMP-3-deoxy-D-manno-octulosonate and lipid IV(A): step 2/4. KEYWORDS: Glycosyltransferase;Lipid A biosynthesis;Lipid biosynthesis;Lipid metabolism;Mitochondrion;Reference proteome;Transferase;Transit peptide SUBCELLULAR LOCATION: Mitochondrion " Q8VZH2,PROTEIN NAMES: Aminopeptidase M1 (Alpha-aminoacylpeptide hydrolase) PROTEIN FAMILY: Peptidase M1 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the peptidase M1 family. FUNCTION: Metallopeptidase that binds to the auxin transport inhibitor N-1-naphthylphthalamic acid (NPA). Required for embryonic and seedling development as well as cell cycle progression. Homodimerization is required to proper localization and activity. May play a negative role in the regulation of PIN auxin transport proteins. KEYWORDS: Aminopeptidase;Cytoplasm;Direct protein sequencing;Endoplasmic reticulum;Hydrolase;Membrane;Metal-binding;Metalloprotease;Microsome;Protease;Reference proteome;Zinc SUBCELLULAR LOCATION: Membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein. Cytoplasm. Note=The dileucine internalization motif may be involved in intracellular sequestration. Q8VZJ1,"PROTEIN NAMES: Histone-lysine N-methyltransferase ATXR5 (Protein SET DOMAIN GROUP 15) (Trithorax-related protein 5) (TRX-related protein 5) PROTEIN FAMILY: Class V-like SAM-binding methyltransferase superfamily, Histone-lysine methyltransferase family, TRX/MLL subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. TRX/MLL subfamily. FUNCTION: Histone methyltransferase that specifically monomethylates 'Lys-27' of histone H3 (H3K27me1). Has much higher activity on nucleosomes containing H3.1 than H3.3. Involved in the formation of constitutive heterochromatin and the silencing of heterochromatic elements. Influences which sets of rRNA gene variants are expressed or silenced. KEYWORDS: Alternative splicing;Chloroplast;Chromatin regulator;Metal-binding;Methyltransferase;Nucleus;Plastid;Reference proteome;S-adenosyl-L-methionine;Transferase;Transit peptide;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Plastid, chloroplast Note=Never found in plastids and the nucleus within the same cell. MISCELLANEOUS: The binding to histone H3.2 is unaffected by mono-, di, or trimethylation at H3K9, but is strongly reduced by increasing levels of H3K4 methylation.; MISCELLANEOUS: [Isoform 1]: Major isoform." Q8VZS3,"PROTEIN NAMES: Transcription factor HHO2 (MYB-domain transcription factor HHO2) (Protein HRS1 HOMOLOG 2) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Probable transcription factor involved in phosphate homeostasis. Involved in the regulation of the developmental response of lateral roots, acquisition and/or mobilization of phosphate and expression of a subset of genes involved in phosphate sensing and signaling pathway. Is a target of the transcription factor PHR1. KEYWORDS: DNA-binding;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " Q8VZS8,PROTEIN NAMES: Abscisic acid receptor PYL1 (ABI1-binding protein 6) (PYR1-like protein 1) (Regulatory components of ABA receptor 9) PROTEIN FAMILY: PYR/PYL/RCAR abscisic acid intracellular receptor family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the PYR/PYL/RCAR abscisic acid intracellular receptor family. FUNCTION: Receptor for abscisic acid (ABA) required for ABA-mediated responses such as stomatal closure and germination inhibition. Inhibits the activity of group-A protein phosphatases type 2C (PP2Cs) when activated by ABA. Can be activated by both (-)-ABA and (+)-ABA. KEYWORDS: 3D-structure;Abscisic acid signaling pathway;Acetylation;Cell membrane;Cytoplasm;Membrane;Nucleus;Protein phosphatase inhibitor;Receptor;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Nucleus Cell membrane Note=Localizes at the plasma membrane in the presence of a CAR protein (e.g. CAR1 and CAR4). MISCELLANEOUS: The synthetic growth inhibitor pyrabactin inhibits ABA-binding and subsequent PP2Cs inhibitor properties. Q8VZV9,"PROTEIN NAMES: Protein PLASTID TRANSCRIPTIONALLY ACTIVE 7 (pTAC7) (PEP-associated protein 12) (Protein PIGMENT DEFECTIVE 225) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Essential for chloroplast development, especially for thylakoid formation. Involved in plastid gene expression, probably by maintaining plastid-encoded RNA polymerase (PEP) activity. KEYWORDS: Alternative splicing;Chloroplast;Plastid;Reference proteome;Transcription;Transcription regulation;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast " Q8W033,"PROTEIN NAMES: Aldehyde dehydrogenase family 3 member I1, chloroplastic (AtALDH3) (Ath-ALDH3) PROTEIN FAMILY: Aldehyde dehydrogenase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the aldehyde dehydrogenase family. FUNCTION: Involved in oxidative stress tolerance by detoxifying reactive aldehydes derived from lipid peroxidation. Medium- to long-chain saturated aldehydes are preferred substrates, while the short-chain aldehyde propanal is a weak substrate. Can use both NAD(+) and NADP(+), but the coenzyme preference is substrate dependent. KEYWORDS: Alternative splicing;Chloroplast;Disulfide bond;NAD;Oxidoreductase;Plastid;Reference proteome;Stress response;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast MISCELLANEOUS: Plants overexpressing ALDH3I1 show improved tolerance when exposed to dehydration, salt stress, heavy metals and H(2)O(2)." Q8W104,"PROTEIN NAMES: F-box/LRR-repeat protein 17 (F-box-like protein 17) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Essential protein for male fertility. Component of the SCF(ASK-cullin-F-box) E3 ubiquitin ligase complex SCF(FBL17), which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Enables the switch in cell cycle control leading to male germ cell lineage formation from microspores after meiosis. Targets CDKA-1 inhibitors the degradation specifically in male germ cells (e.g. KRP6 and KRP7) and thus enables CDKA-1 activation and germ cell S-phase progression. Promotes twin sperm cell production and double fertilization. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Cell cycle;Developmental protein;Leucine-rich repeat;Nucleus;Reference proteome;Repeat;Ubl conjugation pathway SUBCELLULAR LOCATION: Nucleus Note=Confined to the male germ cell nucleus of early to mid bicellular pollen. " Q8W1L6,"PROTEIN NAMES: Peroxisomal fatty acid beta-oxidation multifunctional protein (MFP) [Includes: Enoyl-CoA hydratase/3-2-trans-enoyl-CoA isomerase/3-hydroxybutyryl-CoA epimerase ; 3-hydroxyacyl-CoA dehydrogenase ] PROTEIN FAMILY: Enoyl-CoA hydratase/isomerase family; 3-hydroxyacyl-CoA dehydrogenase family ORGANISM: Oryza sativa subsp. japonica (Rice) SIMILARITY: In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.; SIMILARITY: In the central section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family. FUNCTION: Multifunctional enzyme involved in fatty acid beta-oxidation. Also binds to RNA and microtubules. Possible role in subcellular mRNA localization and RNA-cytoskeleton interactions. PATHWAY: Lipid metabolism; fatty acid beta-oxidation. KEYWORDS: Cytoplasm;Cytoskeleton;Direct protein sequencing;Fatty acid metabolism;Isomerase;Lipid metabolism;Lyase;Microtubule;Multifunctional enzyme;NAD;Oxidoreductase;Peroxisome;Reference proteome;RNA-binding SUBCELLULAR LOCATION: Peroxisome Cytoplasm, cytoskeleton " Q8W3L1,"PROTEIN NAMES: NADPH:adrenodoxin oxidoreductase, mitochondrial (Adrenodoxin reductase) (Mitochondrial ferredoxin reductase) (AtMFDR) PROTEIN FAMILY: Ferredoxin--NADP reductase type 1 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the ferredoxin--NADP reductase type 1 family. FUNCTION: Associates in vitro with the adrenodoxin-like protein MFDX1 to form an efficient low potential electron transfer chain that is able to reduce cytochrome C. Functions as accessory mitochondrial protein involved with BIO2 in the plant biotin synthase reaction. KEYWORDS: Biotin biosynthesis;Electron transport;FAD;Flavoprotein;Mitochondrion;NADP;Oxidoreductase;Reference proteome;Transit peptide;Transport SUBCELLULAR LOCATION: Mitochondrion " Q8W468,"PROTEIN NAMES: Probable E3 ubiquitin-protein ligase ARI8 (ARIADNE-like protein ARI8) (Protein ariadne homolog 8) (RING-type E3 ubiquitin transferase ARI8) PROTEIN FAMILY: RBR family, Ariadne subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the RBR family. Ariadne subfamily. FUNCTION: Might act as an E3 ubiquitin-protein ligase, or as part of E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes and then transfers it to substrates. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Metal-binding;Reference proteome;Repeat;Transferase;Ubl conjugation pathway;Zinc;Zinc-finger " Q8W4B2,"PROTEIN NAMES: Protein APEM9 (ABERRANT PEROXISOME MORPHOLOGY 9) (Protein DAYU) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Involved in peroxisome biogenesis and matrix protein import. Required for pollen maturation and in vivo germination via its role in peroxisomal function, which partially involves jasmonic acid biosynthesis. Transported to peroxisomes via the interaction with PEX19-1. Required for peroxisomal protein import by acting as an anchoring protein for the AAA ATPase complex, which consists of PEX1 and PEX6. KEYWORDS: Membrane;Peroxisome;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Peroxisome membrane ; Multi-pass membrane protein " Q8W4D6,"PROTEIN NAMES: Protein HIGH CHLOROPHYLL FLUORESCENCE PHENOTYPE 173, chloroplastic PROTEIN FAMILY: NmrA-type oxidoreductase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the NmrA-type oxidoreductase family. FUNCTION: Auxiliary factor required, together with HCF244, for the biogenesis of photosystem II (PSII), especially for the synthesis of the reaction center proteins (e.g. D1), via the regulation of the corresponding mRNA (e.g. psbA) translation initiation (ribosomal loading) and stabilization. KEYWORDS: Chloroplast;Membrane;Photosynthesis;Photosystem II;Plastid;Reference proteome;Thylakoid;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast membrane Plastid, chloroplast thylakoid membrane ; Peripheral membrane protein ; Stromal side Plastid, chloroplast stroma Note=Predominantly present at thylakoid membranes. " Q8W4E7,"PROTEIN NAMES: Solute carrier family 40 member 3, chloroplastic (Ferroportin-3) (Iron-regulated transporter 3) (AtIREG3) (Protein MULTIPLE ANTIBIOTIC RESISTANCE 1) PROTEIN FAMILY: Ferroportin (FP) family, SLC40A subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the ferroportin (FP) (TC 2.A.100) family. SLC40A subfamily. FUNCTION: Probable plastid transporter that may play a role in iron chelation, storage or sequestration under limiting iron conditions. In presence of exogenous antibiotics, may allow opportunistic entry of multiple aminoglycoside antibiotics into the chloroplast. KEYWORDS: Chloroplast;Ion transport;Membrane;Plastid;Reference proteome;Transit peptide;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Membrane ; Multi-pass membrane protein Plastid, chloroplast envelope. " Q8W4I6,"PROTEIN NAMES: GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic (Protein BRZ-INSENSITIVE-PALE GREEN 2) PROTEIN FAMILY: TRAFAC class YlqF/YawG GTPase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the TRAFAC class YlqF/YawG GTPase family. FUNCTION: Required for brassinosteroid- (BR) mediated post-transcriptional and translational regulation in the chloroplast, including accumulation of chloroplast rRNA. Involved in chloroplast differentiation. KEYWORDS: Brassinosteroid signaling pathway;Chloroplast;GTP-binding;Hydrolase;Nucleotide-binding;Plastid;Reference proteome;RNA-binding;rRNA processing;rRNA-binding;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast stroma " Q8W4L5,"PROTEIN NAMES: B3 domain-containing transcription repressor VAL1 (Protein HIGH-LEVEL EXPRESSION OF SUGAR-INDUCIBLE 2) (Protein VP1/ABI3-LIKE 1) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Transcriptional repressor of gene expression involved in embryonic pathways, such as LEC1, ABI3, and FUS3. Repressor of the sugar-inducible genes involved in the seed maturation program in seedlings. Plays an essential role in regulating the transition from seed maturation to seedling growth. Functionally redundant with VAL2/HSL1. KEYWORDS: 3D-structure;Coiled coil;DNA-binding;Metal-binding;Nucleus;Reference proteome;Repressor;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus " Q8W5B1,"PROTEIN NAMES: Polycomb group protein VERNALIZATION 2 PROTEIN FAMILY: VEFS (VRN2-EMF2-FIS2-SU(Z)12) family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the VEFS (VRN2-EMF2-FIS2-SU(Z)12) family. FUNCTION: Polycomb group (PcG) protein. Plays a central role in vernalization by maintaining repressed the homeotic gene FLC, a floral repressor, after a cold treatment. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development. PcG proteins are not required to initiate repression, but to maintain it during later stages of development. They probably act via the methylation of histones, rendering chromatin heritably changed in its expressibility. Associates constitutively along the whole FLC locus. KEYWORDS: Alternative splicing;Metal-binding;Nucleus;Reference proteome;Repressor;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus. " Q8WP15,"PROTEIN NAMES: Rhodopsin kinase GRK7 (G protein-coupled receptor kinase 7) (G protein-coupled receptor kinase GRK7) PROTEIN FAMILY: Protein kinase superfamily, AGC Ser/Thr protein kinase family, GPRK subfamily ORGANISM: Sus scrofa (Pig) SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily. FUNCTION: Retina-specific kinase involved in the shutoff of the photoresponse and adaptation to changing light conditions via cone opsin phosphorylation, including rhodopsin (RHO). KEYWORDS: ATP-binding;Kinase;Lipoprotein;Membrane;Methylation;Nucleotide-binding;Phosphoprotein;Prenylation;Reference proteome;Sensory transduction;Serine/threonine-protein kinase;Transferase;Vision SUBCELLULAR LOCATION: Membrane ; Lipid-anchor MISCELLANEOUS: Although the protein is present in a diversity of vertebrates ranging from bony fish to mammals, the mouse and rat orthologous proteins do not exist." Q8WPJ2,"PROTEIN NAMES: Mannan endo-1,4-beta-mannosidase (Beta-mannanase) (Endo-beta-1,4-mannanase) (Man5A) (ManA) PROTEIN FAMILY: Glycosyl hydrolase 5 (cellulase A) family ORGANISM: Mytilus edulis (Blue mussel) SIMILARITY: Belongs to the glycosyl hydrolase 5 (cellulase A) family. FUNCTION: Hydrolyzes 1,4-beta linked polysaccharide backbones of mannans. Hydrolyzes mannohexaose (M6) preferentially to mannotriose (M4) and less preferentially to mannotetraose (M3), mannopentaose (M5), and mannobiose (M2); hydrolyzes M5 preferentially to M2, and M3, and less preferentially to mannotetraose M4; hydrolyzes M4 preferentially to M3, and less preferentially to mannose (M1), plus very little M2. Does not hydrolyze mannobiose or mannotriose. Does not hydrolyze xlyan, starch, cellulose or galactose. KEYWORDS: 3D-structure;Direct protein sequencing;Disulfide bond;Glycosidase;Hydrolase;Signal " Q8WQ86,"PROTEIN NAMES: Microtubule-associated protein RP/EB family member 1 (DdEB1) PROTEIN FAMILY: MAPRE family ORGANISM: Dictyostelium discoideum (Social amoeba) SIMILARITY: Belongs to the MAPRE family. FUNCTION: Involved in microtubule polymerization, and spindle function by stabilizing microtubules and anchoring them at centrosomes. KEYWORDS: Cell cycle;Cell division;Cell projection;Centromere;Chromosome;Coiled coil;Cytoplasm;Cytoskeleton;Kinetochore;Microtubule;Mitosis;Reference proteome SUBCELLULAR LOCATION: Cell projection Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Cytoplasm Chromosome, centromere, kinetochore Note=Associated with the microtubule network. Accumulates at the plus end of microtubules. " Q8WQA4,"PROTEIN NAMES: Chloride intracellular channel exc-4 (Excretory canal abnormal protein 4) PROTEIN FAMILY: Chloride channel CLIC family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the chloride channel CLIC family. FUNCTION: May insert into membranes and form chloride ion channels. Involved in the formation of the excretory canal. Required to prevent cystic lumenal expansions in the excretory cell. Not required for formation of the initial tube, but is required for regulating the size of the tube lumen as it grows. KEYWORDS: 3D-structure;Chloride;Chloride channel;Cytoplasm;Ion channel;Ion transport;Membrane;Reference proteome;Transmembrane;Transmembrane helix;Transport;Voltage-gated channel SUBCELLULAR LOCATION: Cytoplasm. Membrane ; Single-pass membrane protein Note=Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain. Localizes to various tubular membranes in distinct cell types, including the lumenal membrane of the excretory tubes. " Q8WR51,"PROTEIN NAMES: GDP-fucose protein O-fucosyltransferase 2 (Patterning defective protein 2) (Peptide-O-fucosyltransferase 2) (O-FucT-2) PROTEIN FAMILY: Glycosyltransferase 68 family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the glycosyltransferase 68 family. FUNCTION: Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue in the consensus sequence C1-X-X-S/T-C2 of thrombospondin type I repeats (TSRs) where C1 and C2 are the first and second cysteines of the repeat, respectively. O-fucosylates members of several protein families including the ADAMTS superfamily and the thrombospondin (TSP) and spondin families (By similarity). PATHWAY: Protein modification; protein glycosylation. KEYWORDS: 3D-structure;Carbohydrate metabolism;Disulfide bond;Endoplasmic reticulum;Fucose metabolism;Glycoprotein;Glycosyltransferase;Golgi apparatus;Reference proteome;Signal;Transferase SUBCELLULAR LOCATION: Endoplasmic reticulum Golgi apparatus Note=Mainly located in the endoplasmic reticulum. " Q8WTT0,PROTEIN NAMES: C-type lectin domain family 4 member C (Blood dendritic cell antigen 2) (BDCA-2) (C-type lectin superfamily member 7) (Dendritic lectin) (CD antigen CD303) ORGANISM: Homo sapiens (Human) FUNCTION: Lectin-type cell surface receptor which may play a role in antigen capturing by dendritic cells. Specifically recognizes non-sialylated galactose-terminated biantennary glycans containing the trisaccharide epitope Gal(beta1-3/4)GlcNAc(beta1-2)Man. Binds to serum IgG. Efficiently targets ligand into antigen-processing and peptide-loading compartments for presentation to T-cells. May mediate potent inhibition of induction of IFN-alpha/beta expression in plasmacytoid dendritic cells. May act as a signaling receptor that activates protein-tyrosine kinases and mobilizes intracellular calcium. KEYWORDS: 3D-structure;Adaptive immunity;Alternative splicing;Calcium;Cell membrane;Disulfide bond;Glycoprotein;Immunity;Innate immunity;Lectin;Membrane;Metal-binding;Reference proteome;Signal-anchor;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type II membrane protein Q8WU03,"PROTEIN NAMES: Glycine N-acyltransferase-like protein 2 (Acyl-CoA:glycine N-acyltransferase-like protein 2) PROTEIN FAMILY: Glycine N-acyltransferase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the glycine N-acyltransferase family. FUNCTION: Mitochondrial acyltransferase which transfers the acyl group to the N-terminus of glycine. Conjugates numerous substrates, such as arachidonoyl-CoA and saturated medium and long-chain acyl-CoAs ranging from chain-length C8:0-CoA to C18:0-CoA, to form a variety of N-acylglycines. Shows a preference for monounsaturated fatty acid oleoyl-CoA (C18:1-CoA) as an acyl donor. Does not exhibit any activity toward C22:6-CoA and chenodeoxycholoyl-CoA, nor toward serine or alanine. KEYWORDS: Acetylation;Acyltransferase;Endoplasmic reticulum;Lipid metabolism;Reference proteome;Transferase SUBCELLULAR LOCATION: Endoplasmic reticulum " Q8WUA7,PROTEIN NAMES: TBC1 domain family member 22A ORGANISM: Homo sapiens (Human) FUNCTION: May act as a GTPase-activating protein for Rab family protein(s). KEYWORDS: 3D-structure;Acetylation;Alternative splicing;Direct protein sequencing;GTPase activation;Phosphoprotein;Reference proteome Q8WUF5,"PROTEIN NAMES: RelA-associated inhibitor (Inhibitor of ASPP protein) (Protein iASPP) (NFkB-interacting protein 1) (PPP1R13B-like protein) PROTEIN FAMILY: IASPP family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the iASPP family. FUNCTION: Regulator that plays a central role in regulation of apoptosis and transcription via its interaction with NF-kappa-B and p53/TP53 proteins. Blocks transcription of HIV-1 virus by inhibiting the action of both NF-kappa-B and SP1. Also inhibits p53/TP53 function, possibly by preventing the association between p53/TP53 and ASPP1 or ASPP2, and therefore suppressing the subsequent activation of apoptosis. Is involved in NF-kappa-B dependent negative regulation of inflammatory response. KEYWORDS: 3D-structure;Acetylation;ANK repeat;Apoptosis;Cardiomyopathy;Cytoplasm;Disease variant;Ectodermal dysplasia;Methylation;Nucleus;Phosphoprotein;Reference proteome;Repeat;Repressor;SH3 domain;Transcription;Transcription regulation SUBCELLULAR LOCATION: Cytoplasm Nucleus Note=Predominantly cytoplasmic but also nuclear. " Q8WUU4,PROTEIN NAMES: Zinc finger protein 296 (ZFP296) (Zinc finger protein 342) PROTEIN FAMILY: Krueppel C2H2-type zinc-finger protein family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein family. FUNCTION: May be a transcriptional corepressor with KLF4. KEYWORDS: Isopeptide bond;Metal-binding;Nucleus;Reference proteome;Repeat;Transcription;Transcription regulation;Ubl conjugation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Q8WVF1,PROTEIN NAMES: Protein OSCP1 (hOSCP1) (Organic solute transport protein 1) (Oxidored-nitro domain-containing protein 1) ORGANISM: Homo sapiens (Human) FUNCTION: May be involved in drug clearance in the placenta. KEYWORDS: Alternative splicing;Cell membrane;Membrane;Reference proteome;Transport SUBCELLULAR LOCATION: Basal cell membrane. Note=Syncytiotrophoblast in placenta. MISCELLANEOUS: May be involved in the development and/or progression of nosopharyngeal carcinoma. Q8WVZ9,"PROTEIN NAMES: Kelch repeat and BTB domain-containing protein 7 ORGANISM: Homo sapiens (Human) FUNCTION: As part of the CUL3(KBTBD6/7) E3 ubiquitin ligase complex functions as a substrate adapter for the RAC1 guanine exchange factor (GEF) TIAM1, mediating its 'Lys-48' ubiquitination and proteasomal degradation. By controlling this ubiquitination, regulates RAC1 signal transduction and downstream biological processes including the organization of the cytoskeleton, cell migration and cell proliferation. Ubiquitination of TIAM1 requires the membrane-associated protein GABARAP which may restrict locally the activity of the complex. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Cytoplasm;Kelch repeat;Nucleus;Phosphoprotein;Reference proteome;Repeat;Signal transduction inhibitor;Ubl conjugation pathway SUBCELLULAR LOCATION: Cytoplasm Nucleus " Q8WWB7,PROTEIN NAMES: Glycosylated lysosomal membrane protein (Lysosomal protein NCU-G1) PROTEIN FAMILY: GLMP family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the GLMP family. FUNCTION: Required to protect lysosomal transporter MFSD1 from lysosomal proteolysis and for MFSD1 lysosomal localization. KEYWORDS: Alternative splicing;Glycoprotein;Lysosome;Membrane;Reference proteome;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Lysosome membrane ; Single-pass type I membrane protein ; Lumenal side Q8WWI5,"PROTEIN NAMES: Choline transporter-like protein 1 (CDw92) (Solute carrier family 44 member 1) (CD antigen CD92) PROTEIN FAMILY: CTL (choline transporter-like) family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the CTL (choline transporter-like) family. FUNCTION: Choline/H+ antiporter. Also acts as a high-affinity ethanolamine/H+ antiporter, regulating the supply of extracellular ethanolamine (Etn) for the CDP-Etn pathway, redistribute intracellular Etn and balance the CDP-Cho and CDP-Etn arms of the Kennedy pathway. Involved in membrane synthesis and myelin production. KEYWORDS: 3D-structure;Alternative splicing;Antiport;Cell membrane;Lipoprotein;Membrane;Mitochondrion;Mitochondrion outer membrane;Myristate;Neurodegeneration;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Mitochondrion outer membrane ; Multi-pass membrane protein " Q8WWM9,"PROTEIN NAMES: Cytoglobin (Histoglobin) (HGb) (Nitric oxygen dioxygenase CYGB) (NOD) (Nitrite reductase CYGB) (Pseudoperoxidase CYGB) (Stellate cell activation-associated protein) (Superoxide dismutase CYGB) PROTEIN FAMILY: Globin family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the globin family. FUNCTION: Probable multifunctional globin with a hexacoordinated heme iron required for the catalysis of various reactions depending on redox condition of the cell as well as oxygen availability. Has a nitric oxide dioxygenase (NOD) activity and is most probably involved in cell-mediated and oxygen-dependent nitric oxide consumption. By scavenging this second messenger may regulate several biological processes including endothelium-mediated vasodilation and vascular tone. Under normoxic conditions functions as a nitric oxide dioxygenase (NOD) but under hypoxic conditions the globin may switch its function to that of a nitrite (NO2) reductase (NiR), generating nitric oxide. Could also have peroxidase and superoxide dismutase activities, detoxifying reactive oxygen species and protecting cells against oxidative stress. Also binds dioxygen with low affinity and could function as an oxygen sensor but has probably no function as a respiratory oxygen carrier. KEYWORDS: 3D-structure;Cytoplasm;Disulfide bond;Heme;Iron;Metal-binding;Nucleus;Oxidoreductase;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Nucleus " Q8WWP7,"PROTEIN NAMES: GTPase IMAP family member 1 (Immunity-associated protein 1) (hIMAP1) PROTEIN FAMILY: TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily, AIG1/Toc34/Toc159-like paraseptin GTPase family, IAN subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. AIG1/Toc34/Toc159-like paraseptin GTPase family. IAN subfamily. FUNCTION: May regulate lymphocyte survival. Required for normal levels of mature T-lymphocytes and mature B-cells (By similarity). KEYWORDS: 3D-structure;Endoplasmic reticulum;Golgi apparatus;GTP-binding;Membrane;Nucleotide-binding;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Single-pass type IV membrane protein Golgi apparatus membrane ; Single-pass type IV membrane protein " Q8WWR8,"PROTEIN NAMES: Sialidase-4 (N-acetyl-alpha-neuraminidase 4) PROTEIN FAMILY: Glycosyl hydrolase 33 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the glycosyl hydrolase 33 family. FUNCTION: Exo-alpha-sialidase that catalyzes the hydrolytic cleavage of the terminal sialic acid (N-acetylneuraminic acid, Neu5Ac) of a glycan moiety in the catabolism of glycolipids, glycoproteins and oligosacharides. Efficiently hydrolyzes gangliosides including alpha-(2->3)-sialylated GD1a and GM3 and alpha-(2->8)-sialylated GD3. Hydrolyzes poly-alpha-(2->8)-sialylated neural cell adhesion molecule NCAM1 likely at growth cones, suppressing neurite outgrowth in hippocampal neurons (By similarity). May desialylate sialyl Lewis A and X antigens at the cell surface, down-regulating these glycan epitopes recognized by SELE/E selectin in the initiation of cell adhesion and extravasation. Has sialidase activity toward mucin, fetuin and sialyllactose. KEYWORDS: Alternative splicing;Carbohydrate metabolism;Cell membrane;Cell projection;Endoplasmic reticulum;Glycosidase;Hydrolase;Lipid degradation;Lipid metabolism;Lysosome;Membrane;Microsome;Mitochondrion;Mitochondrion inner membrane;Mitochondrion outer membrane;Reference proteome;Repeat SUBCELLULAR LOCATION: [Isoform 1]: Cell membrane ; Peripheral membrane protein. Endoplasmic reticulum membrane ; Peripheral membrane protein. Microsome membrane ; Peripheral membrane protein. Mitochondrion membrane ; Peripheral membrane protein. Cell projection, neuron projection Note=Predominantly associates with endoplasmic reticulum membranes. Only a small fraction associates with mitochondrial and plasma membranes.; SUBCELLULAR LOCATION: [Isoform 2]: Mitochondrion inner membrane ; Peripheral membrane protein. Mitochondrion outer membrane ; Peripheral membrane protein. Lysosome lumen Note=According to previous research is soluble, N-glycosylated and found in the lumen of lysosomes. However, no signal sequence nor N-glycosylation site is predicted from the sequence. " Q8WXD5,"PROTEIN NAMES: Gem-associated protein 6 (Gemin-6) (SIP2) ORGANISM: Homo sapiens (Human) FUNCTION: The SMN complex catalyzes the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome, and thereby plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP (Sm core). In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. To assemble core snRNPs, the SMN complex accepts the trapped 5Sm proteins from CLNS1A forming an intermediate. Binding of snRNA inside 5Sm triggers eviction of the SMN complex, thereby allowing binding of SNRPD3 and SNRPB to complete assembly of the core snRNP. KEYWORDS: 3D-structure;Cytoplasm;Direct protein sequencing;mRNA processing;mRNA splicing;Nucleus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Nucleus, nucleoplasm Nucleus, gem Cytoplasm Note=Found both in the nucleoplasm and in nuclear bodies called gems (Gemini of Cajal bodies) that are often in proximity to Cajal (coiled) bodies. Also found in the cytoplasm. " Q8WXE1,"PROTEIN NAMES: ATR-interacting protein (ATM and Rad3-related-interacting protein) PROTEIN FAMILY: ATRIP family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the ATRIP family. FUNCTION: Required for checkpoint signaling after DNA damage. Required for ATR expression, possibly by stabilizing the protein. KEYWORDS: 3D-structure;Alternative splicing;Coiled coil;Direct protein sequencing;DNA damage;DNA repair;Nucleus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Nucleus Note=Redistributes to discrete nuclear foci upon DNA damage. " Q8WXI4,PROTEIN NAMES: Acyl-coenzyme A thioesterase 11 (Acyl-CoA thioesterase 11) (Acyl-CoA thioester hydrolase 11) (Adipose-associated thioesterase) (Brown fat-inducible thioesterase) (BFIT) (Palmitoyl-coenzyme A thioesterase) ORGANISM: Homo sapiens (Human) FUNCTION: Has an acyl-CoA thioesterase activity with a preference for the long chain fatty acyl-CoA thioesters hexadecanoyl-CoA/palmitoyl-CoA and tetradecanoyl-CoA/myristoyl-CoA which are the main substrates in the mitochondrial beta-oxidation pathway. PATHWAY: Lipid metabolism; fatty acid metabolism. KEYWORDS: 3D-structure;Alternative splicing;Cytoplasm;Fatty acid metabolism;Hydrolase;Lipid metabolism;Mitochondrion;Phosphoprotein;Reference proteome;Repeat;Serine esterase;Transit peptide SUBCELLULAR LOCATION: Mitochondrion matrix Cytoplasm Q8WXK4,PROTEIN NAMES: Ankyrin repeat and SOCS box protein 12 (ASB-12) PROTEIN FAMILY: Ankyrin SOCS box (ASB) family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the ankyrin SOCS box (ASB) family. FUNCTION: Probable substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Alternative splicing;ANK repeat;Reference proteome;Repeat;Ubl conjugation pathway Q8WYR4,"PROTEIN NAMES: Radial spoke head 1 homolog (Cancer/testis antigen 79) (CT79) (Male meiotic metaphase chromosome-associated acidic protein) (Meichroacidin) (Testis-specific gene A2 protein) ORGANISM: Homo sapiens (Human) FUNCTION: Functions as part of axonemal radial spoke complexes that play an important part in the motility of sperm and cilia. KEYWORDS: 3D-structure;Alternative splicing;Cell projection;Chromosome;Ciliopathy;Cilium;Cytoplasm;Cytoskeleton;Disease variant;Flagellum;Meiosis;Primary ciliary dyskinesia;Reference proteome;Repeat SUBCELLULAR LOCATION: Cytoplasm Chromosome Cytoplasm, cytoskeleton, cilium axoneme Cytoplasm, cytoskeleton, flagellum axoneme Note=Cytoplasmic in late spermatocytes, secondary spermatocytes and round spermatids. Gathered around metaphase chromosomes during meiotic divisions. " Q8X0Z1,"PROTEIN NAMES: Bifunctional lycopene cyclase/phytoene synthase [Includes: Lycopene beta-cyclase (Carotene cyclase) (Lycopene cyclase); Phytoene synthase ] PROTEIN FAMILY: Lycopene beta-cyclase family; Phytoene/squalene synthase family ORGANISM: Gibberella fujikuroi (Bakanae and foot rot disease fungus) (Fusarium fujikuroi) SIMILARITY: In the N-terminal section; belongs to the lycopene beta-cyclase family.; SIMILARITY: In the C-terminal section; belongs to the phytoene/squalene synthase family. FUNCTION: Bifunctional enzyme; part of the car gene cluster that mediates the biosynthesis of neurosporaxanthin, a carboxylic apocarotenoid acting as an essential protective pigments and leading to orange pigmentation. CarAR catalyzes the first step of the pathway by converting geranylgeranyl diphosphate to phytoene, as well as the later cyclization step that transforms the carB product lycopene into gamma-carotene. CarAR also converts part of gamma-carotene into beta-carotene. Neurosporaxanthin is synthesized from geranyl-geranyl pyrophosphate (GGPP) through several enzymatic activities. Phytoene synthase activity performed by the bifunctional enzyme carAR first produces phytoene from geranyl-geranyl pyrophosphate (GGPP). The phytoene dehydrogenase carB then introduces 4 desaturations to lead to lycopene which is substrate of the carotene cyclase activity of carAR that leads to the production of gamma-carotene. CarB then performs a 5th desaturation reaction to yield torulene. Torulene is the substrate of the dioxidase carT that breaks the molecule, removing five carbon atoms to yield beta-apo-4'-carotenal, whereas the aldehyde dehydrogenase carD mediates the last step by converting beta-apo-4'-carotenal into neurosporaxanthin (Probable). PATHWAY: Carotenoid biosynthesis; beta-carotene biosynthesis.; PATHWAY: Carotenoid biosynthesis; phytoene biosynthesis; all-trans-phytoene from geranylgeranyl diphosphate: step 1/1. KEYWORDS: Carotenoid biosynthesis;Isomerase;Membrane;Multifunctional enzyme;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Multi-pass membrane protein " Q8X1D8,"PROTEIN NAMES: Nitroalkane oxidase (NAO) PROTEIN FAMILY: Acyl-CoA dehydrogenase family ORGANISM: Fusarium oxysporum (Fusarium vascular wilt) SIMILARITY: Belongs to the acyl-CoA dehydrogenase family. FUNCTION: Catalyzes the oxidative denitrification of neutral nitroalkanes, including 3-nitro-2-pentanol, 1-nitropropane, 2-nitropropane, nitroethane and nitrocyclohexane, and may thereby protect the organism against toxic compounds. Has no detectable acyl-CoA dehydrogenase activity. KEYWORDS: 3D-structure;Direct protein sequencing;FAD;Flavoprotein;Nucleotide-binding;Oxidoreductase " Q8X225,"PROTEIN NAMES: Histone-lysine N-methyltransferase, H3 lysine-9 specific dim-5 (Histone H3-K9 methyltransferase dim-5) (H3-K9-HMTase dim-5) (HKMT) PROTEIN FAMILY: Class V-like SAM-binding methyltransferase superfamily, Histone-lysine methyltransferase family, Suvar3-9 subfamily ORGANISM: Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) SIMILARITY: Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. Suvar3-9 subfamily. FUNCTION: Histone methyltransferase that specifically trimethylates histone H3 to form H3K9me3. H3K9me3 marks chromatin regions for DNA methylation. Dim-5 recognizes Arg-8 to Gly-12 of the H3 tail with Thr-11 and Gly-12 being the most important specificity determinants, the recognition of whcih is important to distinguish H3K9 from H3K27 and H4K20. KEYWORDS: 3D-structure;Chromosome;Metal-binding;Methyltransferase;Nucleus;Reference proteome;S-adenosyl-L-methionine;Transferase;Zinc SUBCELLULAR LOCATION: Nucleus Chromosome " Q8ZKR2,"PROTEIN NAMES: Aminoimidazole riboside kinase (AIRs kinase) PROTEIN FAMILY: Carbohydrate kinase PfkB family ORGANISM: Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) SIMILARITY: Belongs to the carbohydrate kinase PfkB family. FUNCTION: Phosphorylates 5-amino-1-(beta-D-ribosyl)imidazole (AIRs) to form 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole (AIR), an important intermediate in the purine and thiamine biosynthetic pathways. It allows the use of exogenous aminoimidazole riboside (AIRs) to satisfy the cellular requirement for purines and thiamine. KEYWORDS: 3D-structure;ATP-binding;Kinase;Metal-binding;Nucleotide-binding;Potassium;Reference proteome;Transferase " Q8ZL58,PROTEIN NAMES: L-talarate/galactarate dehydratase (TalrD/GalrD) (StTGD) PROTEIN FAMILY: Mandelate racemase/muconate lactonizing enzyme family ORGANISM: Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) SIMILARITY: Belongs to the mandelate racemase/muconate lactonizing enzyme family. FUNCTION: Catalyzes the efficient dehydration of both L-talarate (also called L-altrarate) and galactarate to 5-keto-4-deoxy-D-glucarate (5-KDG). Also catalyzes the epimerization of L-talarate to galactarate; epimerization occurs in competition with dehydration. Is required for the utilization of L-talarate as a carbon source. Also functions in galactarate utilization. Is not active on other acid sugars. KEYWORDS: 3D-structure;Lyase;Magnesium;Metal-binding;Reference proteome MISCELLANEOUS: The enzyme product is the enantiomer of the product obtained in the galactarate dehydratase reaction catalyzed by OB2843; the enzymes thus differ in their regiochemistry of dehydration. Q8ZPX9,"PROTEIN NAMES: Aminoglycoside (3'') (9) adenylyltransferase (AadA) (Aminoglycoside nucleotidyltransferase (3'') (9) adenyltransferase) (ANT(3'') (9) adenyltransferase) ORGANISM: Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) FUNCTION: Mediates bacterial resistance to the antibiotics streptomycin and spectinomycin, does not confer resistance to kanamycin. Binds ATP first, then antibiotic. KEYWORDS: 3D-structure;Antibiotic resistance;ATP-binding;Magnesium;Metal-binding;Nucleotide-binding;Nucleotidyltransferase;Reference proteome;Transferase " Q90460,"PROTEIN NAMES: CD166 antigen homolog A (Activated leukocyte cell adhesion molecule A) (DM-GRASP homolog) (Neurolin) (CD antigen CD166) ORGANISM: Danio rerio (Zebrafish) (Brachydanio rerio) FUNCTION: Cell adhesion molecule that mediates both heterotypic cell-cell contacts via its interaction with CD6, as well as homotypic cell-cell contacts. Promotes T-cell activation and proliferation via its interactions with CD6 (By similarity). Contributes to the formation and maturation of the immunological synapse via its interactions with CD6 (By similarity). Mediates homotypic interactions with cells that express ALCAM. Mediates attachment of dendritic cells onto endothelial cells via homotypic interaction. Inhibits endothelial cell migration and promotes endothelial tube formation via homotypic interactions. Required for normal organization of the lymph vessel network. Required for normal hematopoietic stem cell engraftment in the bone marrow. Plays a role in hematopoiesis; required for normal numbers of hematopoietic stem cells in bone marrow. Promotes in vitro osteoblast proliferation and differentiation (By similarity). Promotes neurite extension, axon growth and axon guidance; axons grow preferentially on surfaces that contain ALCAM (By similarity). Mediates outgrowth and pathfinding for retinal ganglion cell axons (By similarity). KEYWORDS: Adaptive immunity;Cell adhesion;Cell membrane;Cell projection;Developmental protein;Disulfide bond;Glycoprotein;Immunity;Immunoglobulin domain;Membrane;Reference proteome;Repeat;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein Cell projection, axon Cell projection, dendrite Note=Detected at the immunological synapse, i.e, at the contact zone between antigen-presenting dendritic cells and T-cells. Colocalizes with CD6 and the TCR/CD3 complex at the immunological synapse. " Q90611,"PROTEIN NAMES: 72 kDa type IV collagenase (72 kDa gelatinase) (Gelatinase A) (Matrix metalloproteinase-2) (MMP-2) PROTEIN FAMILY: Peptidase M10A family ORGANISM: Gallus gallus (Chicken) SIMILARITY: Belongs to the peptidase M10A family. KEYWORDS: Calcium;Collagen degradation;Direct protein sequencing;Disulfide bond;Extracellular matrix;Hydrolase;Metal-binding;Metalloprotease;Protease;Reference proteome;Repeat;Secreted;Signal;Zinc;Zymogen SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix " Q90YM5,"PROTEIN NAMES: Organic solute transporter subunit alpha (OST-alpha) (Solute carrier family 51 subunit alpha) PROTEIN FAMILY: OST-alpha family ORGANISM: Leucoraja erinacea (Little skate) (Raja erinacea) SIMILARITY: Belongs to the OST-alpha family. FUNCTION: Essential component of the Ost-alpha/Ost-beta complex, a heterodimer that acts as the intestinal basolateral transporter responsible for the translocation of bile acids (such as taurocholate), steroids (such as estrone sulfate), and eicosanoids (such as prostaglandin E2). KEYWORDS: Cell membrane;Endoplasmic reticulum;Glycoprotein;Lipid transport;Membrane;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Endoplasmic reticulum membrane ; Multi-pass membrane protein " Q90Z80,"PROTEIN NAMES: F-box only protein 5-A (Early mitotic inhibitor 1-A) ORGANISM: Xenopus laevis (African clawed frog) FUNCTION: Regulates progression through early mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). Binds to the APC activator cdc20 to prevent APC activation. Can also bind directly to the APC to inhibit substrate-binding. Required to arrest unfertilized eggs at metaphase of meiosis II, by preventing their release from metaphase of meiosis II, through inhibition of APC-dependent cyclin B destruction leading to stabilization of cyclin B-cdk1 complex activity. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Cell cycle;Cell division;Cytoplasm;Cytoskeleton;Metal-binding;Mitosis;Nucleus;Reference proteome;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Cytoplasm Cytoplasm, cytoskeleton, spindle Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Note=In interphase, localizes in a punctate manner in the nucleus and cytoplasm with some perinuclear concentration. In mitotic cells, localizes throughout the cell, particularly at the spindle. At metaphase, localized at mitotic centrosomes. Decreases centrosome localization as cells progressed through telophase. " Q90ZE2,"PROTEIN NAMES: Zinc finger protein 703 (NocA-like zinc finger protein 1) (NocA-related zinc finger protein 1) PROTEIN FAMILY: Elbow/Noc family ORGANISM: Danio rerio (Zebrafish) (Brachydanio rerio) SIMILARITY: Belongs to the Elbow/Noc family. FUNCTION: Transcriptional corepressor which does not bind directly to DNA and may regulate transcription through recruitment of histone deacetylases to gene promoters. Required for segmental gene expression during hindbrain development. May regulate cell adhesion, migration and proliferation. KEYWORDS: Alternative initiation;Cytoplasm;Developmental protein;Metal-binding;Nucleus;Reference proteome;Repressor;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Cytoplasm " Q91049,"PROTEIN NAMES: Occludin PROTEIN FAMILY: ELL/occludin family ORGANISM: Gallus gallus (Chicken) SIMILARITY: Belongs to the ELL/occludin family. FUNCTION: May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. Interacts with ZO-1. KEYWORDS: Cell junction;Cell membrane;Coiled coil;Disulfide bond;Membrane;Phosphoprotein;Reference proteome;Tight junction;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Cell junction, tight junction " Q91619,"PROTEIN NAMES: Nodal homolog (Nodal-related protein 1) (Xnr-1) (Xnr1) (nr-1) PROTEIN FAMILY: TGF-beta family ORGANISM: Xenopus laevis (African clawed frog) SIMILARITY: Belongs to the TGF-beta family. FUNCTION: Cooperation and regulatory loops of multiple nodals are essential for mesendoderm patterning in early embryos. Essential for mesoderm formation and axial patterning during embryonic development. Activates the activin-like signaling pathway to induce dorsal and ventral mesoderm in animal cap ectoderm. In addition, also dorsalizes ventral marginal zone (VMZ) tissues during gastrulation. Acts in a downstream signaling cascade via cripto and cer1 to mediate cardiogenesis in embryonic mesoderm. Directs the orientation of the left-right axis by driving the left-specific gene cascade in the left lateral plate mesoderm. KEYWORDS: Cleavage on pair of basic residues;Cytokine;Developmental protein;Disulfide bond;Glycoprotein;Growth factor;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted " Q91VW9,"PROTEIN NAMES: Zinc finger protein with KRAB and SCAN domains 3 (SCAN-KRAB-zinc finger protein) (Zinc finger protein 306) (Zinc finger protein 307) (Zinc finger protein 47 homolog) (Zf47) (Zfp-47) PROTEIN FAMILY: Krueppel C2H2-type zinc-finger protein family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein family. FUNCTION: Transcriptional factor that binds to the consensus sequence 5'-[GT][AG][AGT]GGGG-3' and acts as a repressor of autophagy. Specifically represses expression of genes involved in autophagy and lysosome biogenesis/function such as MAP1LC3B, ULK1 or WIPI2. Associates with chromatin at the ITGB4 and VEGF promoters (By similarity). KEYWORDS: Activator;Autophagy;Cytoplasm;DNA-binding;Isopeptide bond;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Repeat;Repressor;Transcription;Transcription regulation;Ubl conjugation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Cytoplasm Note=Mainly localizes in the nucleus. Under starvation conditions translocates to the cytoplasm, allowing expression of target genes involved in autophagy and lysosome biogenesis/function (By similarity). " Q91W36,"PROTEIN NAMES: Ubiquitin carboxyl-terminal hydrolase 3 (Deubiquitinating enzyme 3) (Ubiquitin thioesterase 3) (Ubiquitin-specific-processing protease 3) PROTEIN FAMILY: Peptidase C19 family, USP3 subfamily ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the peptidase C19 family. USP3 subfamily. FUNCTION: Deubiquitinase that plays a role in several cellular processes including transcriptional regulation, cell cycle progression or innate immunity. In response to DNA damage, deubiquitinates monoubiquitinated target proteins such as histone H2A and H2AX and thereby counteracts RNF168- and RNF8-mediated ubiquitination. In turn, participates in the recruitment of DNA damage repair factors to DNA break sites. Required for proper progression through S phase and subsequent mitotic entry. Acts as a positive regulator of TP53 by deubiquitinating and stabilizing it to promote normal cell proliferation and transformation. Participates in establishing tolerance innate immune memory through non-transcriptional feedback. Mechanistically, negatively regulates TLR-induced NF-kappa-B signaling by targeting and removing the 'Lys-63'-linked polyubiquitin chains on MYD88. Negatively regulates the activation of type I interferon signaling by mediating 'Lys-63'-linked polyubiquitin chains on RIGI and IFIH1. Deubiquitinates also ASC/PYCARD, the central adapter mediating the assembly and activation of most inflammasomes, and thereby promotes inflammasome activation. KEYWORDS: Acetylation;Cell cycle;Chromatin regulator;Cytoplasm;DNA damage;Hydrolase;Metal-binding;Nucleus;Protease;Reference proteome;Thiol protease;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Cytoplasm Note=Localizes preferentially with monoubiquitinated H2A to chromatin. Upon NF-kappa-B signaling activation, exits the nucleus. " Q91X56,"PROTEIN NAMES: Sphingosine 1-phosphate receptor 5 (S1P receptor 5) (S1P5) (Endothelial differentiation G-protein-coupled receptor 8) (Lysophospholipid receptor B4) (Sphingosine 1-phosphate receptor Edg-8) (S1P receptor Edg-8) PROTEIN FAMILY: G-protein coupled receptor 1 family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the G-protein coupled receptor 1 family. FUNCTION: Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. Is coupled to both the G(i/0)alpha and G(12) subclass of heteromeric G-proteins (By similarity). S1P activation on oligodendroglial cells modulates two distinct functional pathways mediating either process retraction or cell survival. S1P activation on O4-positive pre-oligodendrocytes induces process retraction via a Rho kinase/collapsin response-mediated protein signaling pathway. The S1P-induced survival of mature oligodendrocytes is mediated through a pertussis toxin-sensitive, Akt-dependent pathway. S1P activation on oligodendroglial cells modulates two distinct functional pathways mediating either process retraction or cell survival. These effects depend on the developmental stage of the cell. KEYWORDS: Cell membrane;G-protein coupled receptor;Glycoprotein;Lipoprotein;Membrane;Palmitate;Phosphoprotein;Receptor;Reference proteome;Transducer;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. " Q91Y44,"PROTEIN NAMES: Bromodomain testis-specific protein (Bromodomain-containing female sterile homeotic-like protein) (RING3-like protein) PROTEIN FAMILY: BET family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the BET family. FUNCTION: Testis-specific chromatin protein that specifically binds histone H4 acetylated at 'Lys-5' and 'Lys-8' (H4K5ac and H4K8ac, respectively) and plays a key role in spermatogenesis. Required in late pachytene spermatocytes: plays a role in meiotic and post-meiotic cells by binding to acetylated histones at the promoter of specific meiotic and post-meiotic genes, facilitating their activation at the appropriate time. In the post-meiotic phase of spermatogenesis, binds to hyperacetylated histones and participates in their general removal from DNA. Also recognizes and binds a subset of butyrylated histones: able to bind histone H4 butyrylated at 'Lys-8' (H4K8ac), while it is not able to bind H4 butyrylated at 'Lys-5' (H4K5ac). Also acts as a component of the splicing machinery in pachytene spermatocytes and round spermatids and participates in 3'-UTR truncation of specific mRNAs in post-meiotic spermatids. Required for chromocenter organization, a structure comprised of peri-centromeric heterochromatin. KEYWORDS: 3D-structure;Activator;Alternative splicing;Bromodomain;Chromatin regulator;Coiled coil;Differentiation;Meiosis;mRNA processing;mRNA splicing;Nucleus;Phosphoprotein;Reference proteome;Repeat;Spermatogenesis;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus Note=Detected on chromatin. Excluded from the chromocenter. MISCELLANEOUS: Brdt is a promising target for male contraception. Inhibition by thienodiazepine inhibitor (+)-JQ1 that binds Asn-108, prevents recognition of acetylated histone H4. Treatment of mice with JQ1 reduces seminiferous tubule area, testis size and spermatozoa number and motility without affecting hormone levels. JQ1 causes a complete and reversible contraceptive effect in male mice." Q91YD3,"PROTEIN NAMES: mRNA-decapping enzyme 1A (MAD homolog 4-interacting transcription coactivator 1) (Smad4-interacting transcriptional co-activator) (Transcription factor SMIF) PROTEIN FAMILY: DCP1 family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the DCP1 family. FUNCTION: Necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Contributes to the transactivation of target genes after stimulation by TGFB1 (By similarity). Essential for embryonic development. KEYWORDS: Cytoplasm;Hydrolase;Methylation;Nonsense-mediated mRNA decay;Nucleus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cytoplasm, P-body Nucleus Note=Predominantly cytoplasmic, in processing bodies (PB). Nuclear, after TGFB1 treatment. Translocation to the nucleus depends on interaction with SMAD4 (By similarity). Colocalizes with NANOS3 in the processing bodies. " Q91ZP3,"PROTEIN NAMES: Phosphatidate phosphatase LPIN1 (Fatty liver dystrophy protein) (Lipin-1) PROTEIN FAMILY: Lipin family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the lipin family. FUNCTION: Acts as a magnesium-dependent phosphatidate phosphatase enzyme which catalyzes the conversion of phosphatidic acid to diacylglycerol during triglyceride, phosphatidylcholine and phosphatidylethanolamine biosynthesis and therefore controls the metabolism of fatty acids at different levels. Is involved in adipocyte differentiation. Acts also as nuclear transcriptional coactivator for PPARGC1A/PPARA regulatory pathway to modulate lipid metabolism gene expression.; FUNCTION: [Isoform 1]: Recruited at the mitochondrion outer membrane and is involved in mitochondrial fission by converting phosphatidic acid to diacylglycerol. KEYWORDS: 3D-structure;Acetylation;Alternative splicing;Cytoplasm;Direct protein sequencing;Disease variant;Endoplasmic reticulum;Hydrolase;Isopeptide bond;Membrane;Mitochondrion;Mitochondrion outer membrane;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation;Ubl conjugation SUBCELLULAR LOCATION: [Isoform 1]: Mitochondrion outer membrane Cytoplasm Nucleus membrane Note=Recruited at the mitochondrion outer membrane following phosphatidic acid formation mediated by PLD6. In neuronals cells, isoform 1 is exclusively cytoplasmic. In 3T3-L1 pre-adipocytes, it primarily located in the cytoplasm.; SUBCELLULAR LOCATION: [Isoform 2]: Nucleus Cytoplasm Endoplasmic reticulum membrane Note=Nuclear localization requires both CNEP1R1 and CTDNEP1. In neuronals cells, localized in both the cytoplasm and the nucleus. In 3T3-L1 pre-adipocytes, it is predominantly nuclear. Translocates from the cytosol to the endoplasmic reticulum following acetylation by KAT5 (By similarity). " Q92265,"PROTEIN NAMES: Peroxisome biogenesis factor 10 (Peroxin-10) (Peroxisomal biogenesis factor 10) (Peroxisome assembly protein 10) (Peroxisome assembly protein PAS7) PROTEIN FAMILY: Pex2/pex10/pex12 family ORGANISM: Komagataella pastoris (Yeast) (Pichia pastoris) SIMILARITY: Belongs to the pex2/pex10/pex12 family. FUNCTION: E3 ubiquitin-protein ligase component of a retrotranslocation channel required for peroxisome organization by mediating export of the PEX5 and/or PEX20 receptors from peroxisomes to the cytosol, thereby promoting PEX5 and PEX20 recycling. The retrotranslocation channel is composed of PEX2, PEX10 and PEX12; each subunit contributing transmembrane segments that coassemble into an open channel that specifically allows the passage of PEX5 and/or PEX20 through the peroxisomal membrane (By similarity). PEX10 also regulates PEX5 and/or PEX20 recycling by acting as a E3 ubiquitin-protein ligase (By similarity). When PEX5 or PEX20 recycling is compromised, PEX10 catalyzes polyubiquitination of PEX5 and/or PEX20 during their passage through the retrotranslocation channel, leading to its degradation (By similarity). PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Membrane;Metal-binding;Peroxisome;Peroxisome biogenesis;Protein transport;Transferase;Transmembrane;Transmembrane helix;Transport;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Peroxisome membrane ; Multi-pass membrane protein " Q922H1,"PROTEIN NAMES: Protein arginine N-methyltransferase 3 (Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 3) PROTEIN FAMILY: Class I-like SAM-binding methyltransferase superfamily, Protein arginine N-methyltransferase family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N-methyltransferase family. FUNCTION: Protein-arginine N-methyltransferase that catalyzes both the monomethylation and asymmetric dimethylation of the guanidino nitrogens of arginine residues in target proteins, and therefore falls into the group of type I methyltransferases (By similarity). May regulate retinoic acid synthesis and signaling by inhibiting ALDH1A1 retinal dehydrogenase activity (By similarity). KEYWORDS: 3D-structure;Acetylation;Cytoplasm;Metal-binding;Methyltransferase;Phosphoprotein;Reference proteome;S-adenosyl-L-methionine;Transferase;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cytoplasm " Q92331,PROTEIN NAMES: Vacuolar protein sorting-associated protein 5 (Carboxypeptidase Y-deficient protein 10) (Vacuolar protein-targeting protein 5) PROTEIN FAMILY: Sorting nexin family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the sorting nexin family. FUNCTION: Plays a role in vesicular protein sorting. Required for retention of late Golgi membrane proteins and vacuolar biogenesis. Component of the membrane-associated retromer complex which is essential in endosome-to-Golgi retrograde transport. The VPS5-VPS17 subcomplex may assemble onto the membrane to promote vesicle formation. KEYWORDS: Cytoplasm;Endosome;Golgi apparatus;Membrane;Phosphoprotein;Protein transport;Reference proteome;Transport SUBCELLULAR LOCATION: Cytoplasm. Golgi apparatus membrane ; Peripheral membrane protein ; Cytoplasmic side Endosome membrane ; Peripheral membrane protein ; Cytoplasmic side MISCELLANEOUS: Present with 6120 molecules/cell in log phase SD medium. Q92340,"PROTEIN NAMES: High affinity heme transporter ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) FUNCTION: High affinity heme transporter involved in the assimilation of exogenous heme during conditions of low cellular iron. KEYWORDS: Cell membrane;Glycoprotein;GPI-anchor;Lipoprotein;Membrane;Reference proteome;Signal;Vacuole SUBCELLULAR LOCATION: Cell membrane ; Lipid-anchor, GPI-anchor Vacuole membrane ; Lipid-anchor, GPI-anchor Note=Localizes from the cell membrane to the vacuolar membrane in the presence of heme. " Q92358,"PROTEIN NAMES: Telomere bouquet protein 1 (Meiotic chromosome segregation protein bqt1) (Meiotic recombination protein rec26) (Meiotically up-regulated gene 23 protein) ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) FUNCTION: Involved in chromosome segregation. During meiotic prophase, connects telomeres to the spindle pole body by forming a bridge between the telomere protein rap1 and the spindle pole body protein sad1. KEYWORDS: Cell cycle;Cell division;Chromosome;Chromosome partition;Cytoplasm;Cytoskeleton;Meiosis;Reference proteome;Telomere SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body. Chromosome, telomere. Note=Colocalizes with the telomere cluster during the 'horsetail' stage and then disappears before the first meiotic division. " Q923B3,"PROTEIN NAMES: Neurotrophin receptor-interacting factor 1 (Neurotrophin receptor-interacting factor) (Zinc finger protein 110) PROTEIN FAMILY: Krueppel C2H2-type zinc-finger protein family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein family. FUNCTION: Transcription regulator involved in NGFR/p75(NTR)-mediated apoptosis. Essential component of the NGFR/p75(NTR) apoptotic pathway: upon ligand-binding and subsequent cleavage of NGFR/p75(NTR), binds to the intracellular domain (ICD) cleavage product of NGFR/p75(NTR), translocates to the nucleus and induces apoptosis, possibly by regulating expression of key regulators of apoptosis. Induces NGFR/p75(NTR)-mediated apoptosis in retina and sympathetic neurons. May also regulate expression of neuronal cholesterol biosynthesis genes. Probably acts as a transcription repressor: specifically binds to the 3'-end of zinc-finger coding genes and recruiting chromatin-modifying proteins such as SETDB1 and TRIM28/KAP1, leading to transcription repression. KEYWORDS: Apoptosis;Cytoplasm;DNA-binding;Isopeptide bond;Metal-binding;Nucleus;Reference proteome;Repeat;Repressor;Transcription;Transcription regulation;Ubl conjugation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Translocates into the nucleus following binding to TRAF6 and subsequent ubiquitination at Lys-15. " Q923Z0,PROTEIN NAMES: G-protein coupled receptor family C group 5 member B (Retinoic acid-induced gene 2 protein) (RAIG-2) PROTEIN FAMILY: G-protein coupled receptor 3 family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the G-protein coupled receptor 3 family. FUNCTION: G-protein coupled receptor involved in the regulation of cell volume. KEYWORDS: Cell membrane;Cytoplasmic vesicle;G-protein coupled receptor;Glycoprotein;Membrane;Phosphoprotein;Receptor;Reference proteome;Signal;Transducer;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Cytoplasmic vesicle membrane ; Multi-pass membrane protein Note=Localized in the plasma membrane and perinuclear vesicles. Q92484,"PROTEIN NAMES: Cyclic GMP-AMP phosphodiesterase SMPDL3A (2',3'-cGAMP phosphodiesterase SMPDL3A) (Acid sphingomyelinase-like phosphodiesterase 3a) (ASM-like phosphodiesterase 3a) PROTEIN FAMILY: Acid sphingomyelinase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the acid sphingomyelinase family. FUNCTION: Cyclic-nucleotide phosphodiesterase that acts as a negative regulator of innate immunity by mediating degradation of 2',3'-cGAMP, thereby inhibiting the cGAS-STING signaling. Specifically linearizes 2',3'-cGAMP into 2'5'-bond pGpA and further hydrolyzes pGpA to produce GpA. Also has in vitro nucleotide phosphodiesterase activity with nucleoside triphosphates, such as ATP. Has in vitro activity with p-nitrophenyl-TMP. Has lower activity with nucleoside diphosphates, and no activity with nucleoside monophosphates. Has in vitro activity with CDP-choline, giving rise to CMP and phosphocholine. Has in vitro activity with CDP-ethanolamine. Does not have sphingomyelin phosphodiesterase activity. KEYWORDS: 3D-structure;Alternative splicing;Disulfide bond;Glycoprotein;Hydrolase;Metal-binding;Reference proteome;Secreted;Signal;Zinc SUBCELLULAR LOCATION: Secreted " Q924N9,PROTEIN NAMES: Serine protease 28 (Implantation serine proteinase 1) (ISP-1) (Strypsin) (Tryptase-like proteinase) PROTEIN FAMILY: Peptidase S1 family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the peptidase S1 family. FUNCTION: Involved in embryo hatching and implantation. KEYWORDS: Disulfide bond;Glycoprotein;Hydrolase;Protease;Reference proteome;Secreted;Serine protease;Signal SUBCELLULAR LOCATION: Secreted Note=Secretion into the glandular and uterine lumen may occur as a consequence of progesterone-induced epithelial differentiation. Q924V4,"PROTEIN NAMES: Solute carrier family 15 member 3 (Peptide transporter 3) (Peptide/histidine transporter 2) PROTEIN FAMILY: Major facilitator superfamily, Proton-dependent oligopeptide transporter (POT/PTR) family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the major facilitator superfamily. Proton-dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family. FUNCTION: Proton-coupled amino-acid transporter that transports free histidine and certain di- and tripeptides, and is involved in innate immune response. Also able to transport carnosine (By similarity). Involved in the detection of microbial pathogens by toll-like receptors (TLRs) and NOD-like receptors (NLRs), probably by mediating transport of bacterial peptidoglycans across the endolysosomal membrane: catalyzes the transport of certain bacterial peptidoglycans, such as muramyl dipeptide (MDP), the NOD2 ligand (By similarity). KEYWORDS: Endosome;Glycoprotein;Immunity;Innate immunity;Lysosome;Membrane;Peptide transport;Protein transport;Reference proteome;Symport;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Lysosome membrane ; Multi-pass membrane protein Endosome membrane ; Multi-pass membrane protein " Q92506,"PROTEIN NAMES: (3R)-3-hydroxyacyl-CoA dehydrogenase (17-beta-hydroxysteroid dehydrogenase 8) (17-beta-HSD 8) (HSD17B8) (3-ketoacyl-[acyl-carrier-protein] reductase alpha subunit) (KAR alpha subunit) (3-oxoacyl-[acyl-carrier-protein] reductase) (Estradiol 17-beta-dehydrogenase 8) (Protein Ke6) (Ke6) (Short chain dehydrogenase/reductase family 30C member 1) (Testosterone 17-beta-dehydrogenase 8) PROTEIN FAMILY: Short-chain dehydrogenases/reductases (SDR) family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FUNCTION: Required for the solubility and assembly of the heterotetramer 3-ketoacyl-[acyl carrier protein] (ACP) reductase functional complex (KAR or KAR1) that forms part of the mitochondrial fatty acid synthase (mtFAS). Alpha-subunit of the KAR complex that acts as a scaffold protein required for the stability of carbonyl reductase type-4 (CBR4, beta-subunit of the KAR complex) and for its 3-ketoacyl-ACP reductase activity, thereby participating in mitochondrial fatty acid biosynthesis. Catalyzes the NAD-dependent conversion of (3R)-3-hydroxyacyl-CoA into 3-ketoacyl-CoA (3-oxoacyl-CoA) with no chain length preference; this enzymatic activity is not needed for the KAR function. Prefers (3R)-3-hydroxyacyl-CoA over (3S)-3-hydroxyacyl-CoA and displays enzymatic activity only in the presence of NAD(+). Cooperates with enoyl-CoA hydratase 1 in mitochondria, together they constitute an alternative route to the auxiliary enzyme pathways for the breakdown of Z-PUFA (cis polyunsaturated fatty acid) enoyl-esters (Probable). NAD-dependent 17-beta-hydroxysteroid dehydrogenase with highest activity towards estradiol (17beta-estradiol or E2). Has very low activity towards testosterone and dihydrotestosterone (17beta-hydroxy-5alpha-androstan-3-one). Primarily an oxidative enzyme, it can switch to a reductive mode determined in the appropriate physiologic milieu and catalyze the reduction of estrone (E1) to form biologically active 17beta-estradiol. PATHWAY: Steroid biosynthesis; estrogen biosynthesis.; PATHWAY: Lipid metabolism; fatty acid biosynthesis.; PATHWAY: Lipid metabolism; mitochondrial fatty acid beta-oxidation. KEYWORDS: 3D-structure;Fatty acid biosynthesis;Fatty acid metabolism;Lipid biosynthesis;Lipid metabolism;Mitochondrion;NAD;Oxidoreductase;Phosphoprotein;Reference proteome;Steroid biosynthesis SUBCELLULAR LOCATION: Mitochondrion matrix MISCELLANEOUS: The fatty acyl-CoA dehydrogenase activity is several thousand times higher than the estradiol and testosterone 17beta-hydroxysteroid dehydrogenase conversion." Q92522,PROTEIN NAMES: Histone H1.10 (Histone H1x) PROTEIN FAMILY: Histone H1/H5 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the histone H1/H5 family. FUNCTION: Histones H1 are necessary for the condensation of nucleosome chains into higher-order structures. KEYWORDS: 3D-structure;Acetylation;Chromosome;Citrullination;Direct protein sequencing;DNA-binding;Nucleus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Nucleus. Chromosome. Q92542,"PROTEIN NAMES: Nicastrin PROTEIN FAMILY: Nicastrin family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the nicastrin family. FUNCTION: Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (amyloid-beta precursor protein). The gamma-secretase complex plays a role in Notch and Wnt signaling cascades and regulation of downstream processes via its role in processing key regulatory proteins, and by regulating cytosolic CTNNB1 levels. KEYWORDS: 3D-structure;Alternative splicing;Cytoplasmic vesicle;Disease variant;Disulfide bond;Glycoprotein;Membrane;Notch signaling pathway;Reference proteome;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Single-pass type I membrane protein Cytoplasmic vesicle membrane ; Single-pass type I membrane protein Melanosome Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. " Q92563,"PROTEIN NAMES: Testican-2 (SPARC/osteonectin, CWCV, and Kazal-like domains proteoglycan 2) ORGANISM: Homo sapiens (Human) FUNCTION: May participate in diverse steps of neurogenesis. Binds calcium. KEYWORDS: Alternative splicing;Calcium;Direct protein sequencing;Disulfide bond;Extracellular matrix;Glycoprotein;Heparan sulfate;Phosphoprotein;Proteoglycan;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix " Q92599,"PROTEIN NAMES: Septin-8 PROTEIN FAMILY: TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily, Septin GTPase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family. FUNCTION: Filament-forming cytoskeletal GTPase (By similarity). May play a role in platelet secretion. Seems to participate in the process of SNARE complex formation in synaptic vesicles (By similarity).; FUNCTION: [Isoform 4]: Stabilizes BACE1 protein levels and promotes the sorting and accumulation of BACE1 to the recycling or endosomal compartments, modulating the beta-amyloidogenic processing of APP. KEYWORDS: 3D-structure;Acetylation;Alternative splicing;Cell projection;Coiled coil;Cytoplasm;Cytoplasmic vesicle;Cytoskeleton;GTP-binding;Membrane;Nucleotide-binding;Phosphoprotein;Reference proteome;Synapse SUBCELLULAR LOCATION: Cytoplasm Cytoplasm, cytoskeleton Synapse Cell projection, axon Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane Presynapse Note=Expressed in axons of immature neurons, localizes to synapses in mature neurons (By similarity). In platelets, found in areas surrounding alpha-granules. MISCELLANEOUS: [Isoform 2]: KIAA0202a differs from KIAA0202c at the level of the 3'-UTR." Q925K9,"PROTEIN NAMES: Testis-specific serine/threonine-protein kinase 6 (TSK-6) (TSSK-6) (Testis-specific kinase 6) (Serine/threonine-protein kinase SSTK) (Small serine/threonine kinase) PROTEIN FAMILY: Protein kinase superfamily, CAMK Ser/Thr protein kinase family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. FUNCTION: Required for sperm production and function. Plays a role in DNA condensation during postmeiotic chromatin remodeling. KEYWORDS: 3D-structure;ATP-binding;Developmental protein;Differentiation;Kinase;Magnesium;Metal-binding;Nucleotide-binding;Phosphoprotein;Reference proteome;Serine/threonine-protein kinase;Spermatogenesis;Transferase;Ubl conjugation " Q92611,"PROTEIN NAMES: ER degradation-enhancing alpha-mannosidase-like protein 1 PROTEIN FAMILY: Glycosyl hydrolase 47 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the glycosyl hydrolase 47 family. FUNCTION: Extracts misfolded glycoproteins, but not glycoproteins undergoing productive folding, from the calnexin cycle. It is directly involved in endoplasmic reticulum-associated degradation (ERAD) and targets misfolded glycoproteins for degradation in an N-glycan-independent manner, probably by forming a complex with SEL1L. It has low mannosidase activity, catalyzing mannose trimming from Man8GlcNAc2 to Man7GlcNAc2. KEYWORDS: Alternative splicing;Endoplasmic reticulum;Glycoprotein;Membrane;Reference proteome;Signal-anchor;Transmembrane;Transmembrane helix;Unfolded protein response SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Single-pass type II membrane protein " Q92622,"PROTEIN NAMES: Run domain Beclin-1-interacting and cysteine-rich domain-containing protein (Rubicon) (Beclin-1 associated RUN domain containing protein) (Baron) ORGANISM: Homo sapiens (Human) FUNCTION: Inhibits PIK3C3 activity; under basal conditions negatively regulates PI3K complex II (PI3KC3-C2) function in autophagy. Negatively regulates endosome maturation and degradative endocytic trafficking and impairs autophagosome maturation process. Can sequester UVRAG from association with a class C Vps complex (possibly the HOPS complex) and negatively regulates Rab7 activation.; FUNCTION: Involved in regulation of pathogen-specific host defense of activated macrophages. Following bacterial infection promotes NADH oxidase activity by association with CYBA thereby affecting TLR2 signaling and probably other TLR-NOX pathways. Stabilizes the CYBA:CYBB NADPH oxidase heterodimer, increases its association with TLR2 and its phagosome trafficking to induce antimicrobial burst of ROS and production of inflammatory cytokines. Following fungal or viral infection (implicating CLEC7A (dectin-1)-mediated myeloid cell activation or RIGI-dependent sensing of RNA viruses) negatively regulates pro-inflammatory cytokine production by association with CARD9 and sequestering it from signaling complexes. KEYWORDS: 3D-structure;Alternative splicing;Autophagy;Endocytosis;Endosome;Immunity;Lysosome;Neurodegeneration;Phagocytosis;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Late endosome Lysosome Early endosome Note=Predominantly located in late endosomes/lysosomes, only partially detected in early endosome and not at all in the Golgi apparatus. " Q92738,PROTEIN NAMES: USP6 N-terminal-like protein (Related to the N-terminus of tre) (RN-tre) ORGANISM: Homo sapiens (Human) FUNCTION: Acts as a GTPase-activating protein for RAB5A and RAB43. Involved in receptor trafficking. In complex with EPS8 inhibits internalization of EGFR. Involved in retrograde transport from the endocytic pathway to the Golgi apparatus. Involved in the transport of Shiga toxin from early and recycling endosomes to the trans-Golgi network. Required for structural integrity of the Golgi complex. KEYWORDS: Acetylation;Alternative splicing;Cytoplasmic vesicle;Golgi apparatus;GTPase activation;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Golgi apparatus. Cytoplasmic vesicle. Q92908,"PROTEIN NAMES: Transcription factor GATA-6 (GATA-binding factor 6) ORGANISM: Homo sapiens (Human) FUNCTION: Transcriptional activator. Regulates SEMA3C and PLXNA2. Involved in gene regulation specifically in the gastric epithelium. May regulate genes that protect epithelial cells from bacterial infection. Involved in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression (By similarity). Binds to BMP response element (BMPRE) DNA sequences within cardiac activating regions (By similarity). In human skin, controls several physiological processes contributing to homeostasis of the upper pilosebaceous unit. Triggers ductal and sebaceous differentiation as well as limits cell proliferation and lipid production to prevent hyperseborrhoea. Mediates the effects of retinoic acid on sebocyte proliferation, differentiation and lipid production. Also contributes to immune regulation of sebocytes and antimicrobial responses by modulating the expression of anti-inflammatory genes such as IL10 and pro-inflammatory genes such as IL6, TLR2, TLR4, and IFNG. Activates TGFB1 signaling which controls the interfollicular epidermis fate. KEYWORDS: Activator;Alternative initiation;Atrial septal defect;Disease variant;DNA-binding;Isopeptide bond;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Repeat;Transcription;Transcription regulation;Ubl conjugation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: [Isoform 2]: Produced by alternative initiation at Met-147 of isoform 1." Q92985,"PROTEIN NAMES: Interferon regulatory factor 7 (IRF-7) PROTEIN FAMILY: IRF family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the IRF family. FUNCTION: Key transcriptional regulator of type I interferon (IFN)-dependent immune responses and plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Can efficiently activate both the IFN-beta (IFNB) and the IFN-alpha (IFNA) genes and mediate their induction via both the virus-activated, MyD88-independent pathway and the TLR-activated, MyD88-dependent pathway. Induces transcription of ubiquitin hydrolase USP25 mRNA in response to lipopolysaccharide (LPS) or viral infection in a type I IFN-dependent manner (By similarity). Required during both the early and late phases of the IFN gene induction but is more critical for the late than for the early phase. Exists in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, becomes phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization where along with other coactivators it can activate transcription of the type I IFN and ISG genes. Can also play a role in regulating adaptive immune responses by inducing PSMB9/LMP2 expression, either directly or through induction of IRF1. Binds to the Q promoter (Qp) of EBV nuclear antigen 1 a (EBNA1) and may play a role in the regulation of EBV latency. Can activate distinct gene expression programs in macrophages and regulate the anti-tumor properties of primary macrophages (By similarity). KEYWORDS: 3D-structure;Acetylation;Activator;Alternative splicing;Antiviral defense;Cytoplasm;Disease variant;DNA-binding;Host-virus interaction;Immunity;Innate immunity;Isopeptide bond;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation;Ubl conjugation SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=The phosphorylated and active form accumulates selectively in the nucleus. MISCELLANEOUS: [Isoform C]: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay." Q92994,"PROTEIN NAMES: Transcription factor IIIB 90 kDa subunit (TFIIIB90) (hTFIIIB90) (B-related factor 1) (BRF-1) (hBRF) (TAF3B2) (TATA box-binding protein-associated factor, RNA polymerase III, subunit 2) PROTEIN FAMILY: TFIIB family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the TFIIB family. FUNCTION: General activator of RNA polymerase which utilizes different TFIIIB complexes at structurally distinct promoters. The isoform 1 is involved in the transcription of tRNA, adenovirus VA1, 7SL and 5S RNA. Isoform 2 is required for transcription of the U6 promoter. KEYWORDS: Activator;Alternative splicing;Direct protein sequencing;Disease variant;Dwarfism;Intellectual disability;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Repeat;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus " Q92UV7,PROTEIN NAMES: Phosphonoacetaldehyde dehydrogenase PROTEIN FAMILY: Aldehyde dehydrogenase family ORGANISM: Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti) SIMILARITY: Belongs to the aldehyde dehydrogenase family. FUNCTION: Plays an important role in phosphonate degradation by catalyzing the NAD-dependent conversion of phosphonoacetaldehyde (PnAA) to phosphonoacetate (PnA). Has low in vitro activity with the related compounds phosphonopropionaldehyde (3-oxopropyl phosphonate) and glyceraldehyde 3-phosphate. PATHWAY: Phosphorus metabolism. KEYWORDS: 3D-structure;NAD;Nucleotide-binding;Oxidoreductase;Plasmid;Reference proteome Q93038,"PROTEIN NAMES: Tumor necrosis factor receptor superfamily member 25 (Apo-3) (Apoptosis-inducing receptor AIR) (Apoptosis-mediating receptor DR3) (Apoptosis-mediating receptor TRAMP) (Death receptor 3) (Lymphocyte-associated receptor of death) (LARD) (Protein WSL) (Protein WSL-1) ORGANISM: Homo sapiens (Human) FUNCTION: Receptor for TNFSF12/APO3L/TWEAK. Interacts directly with the adapter TRADD. Mediates activation of NF-kappa-B and induces apoptosis. May play a role in regulating lymphocyte homeostasis. KEYWORDS: 3D-structure;Alternative splicing;Apoptosis;Cell membrane;Disulfide bond;Glycoprotein;Membrane;Receptor;Reference proteome;Repeat;Secreted;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: [Isoform 1]: Cell membrane; Single-pass type I membrane protein.; SUBCELLULAR LOCATION: [Isoform 2]: Cell membrane; Single-pass type I membrane protein.; SUBCELLULAR LOCATION: [Isoform 9]: Cell membrane; Single-pass type I membrane protein.; SUBCELLULAR LOCATION: [Isoform 11]: Cell membrane; Single-pass type I membrane protein.; SUBCELLULAR LOCATION: [Isoform 3]: Secreted.; SUBCELLULAR LOCATION: [Isoform 4]: Secreted.; SUBCELLULAR LOCATION: [Isoform 5]: Secreted.; SUBCELLULAR LOCATION: [Isoform 6]: Secreted.; SUBCELLULAR LOCATION: [Isoform 7]: Secreted.; SUBCELLULAR LOCATION: [Isoform 8]: Secreted.; SUBCELLULAR LOCATION: [Isoform 10]: Secreted.; SUBCELLULAR LOCATION: [Isoform 12]: Secreted. MISCELLANEOUS: [Isoform 3]: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.; MISCELLANEOUS: [Isoform 4]: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.; MISCELLANEOUS: [Isoform 5]: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.; MISCELLANEOUS: [Isoform 6]: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.; MISCELLANEOUS: [Isoform 7]: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.; MISCELLANEOUS: [Isoform 12]: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay." Q93070,"PROTEIN NAMES: Ecto-ADP-ribosyltransferase 4 (ADP-ribosyltransferase C2 and C3 toxin-like 4) (ARTC4) (Dombrock blood group carrier molecule) (Mono(ADP-ribosyl)transferase 4) (NAD(P)(+)--arginine ADP-ribosyltransferase 4) (CD antigen CD297) PROTEIN FAMILY: Arg-specific ADP-ribosyltransferase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the Arg-specific ADP-ribosyltransferase family. KEYWORDS: Blood group antigen;Cell membrane;Disulfide bond;Glycoprotein;Glycosyltransferase;GPI-anchor;Lipoprotein;Membrane;NAD;NADP;Nucleotidyltransferase;Reference proteome;Signal;Transferase SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor, GPI-anchor. " Q93097,"PROTEIN NAMES: Protein Wnt-2b (Protein Wnt-13) PROTEIN FAMILY: Wnt family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the Wnt family. FUNCTION: Ligand for members of the frizzled family of seven transmembrane receptors. Functions in the canonical Wnt/beta-catenin signaling pathway. Plays a redundant role in embryonic lung development. KEYWORDS: Alternative splicing;Developmental protein;Disease variant;Disulfide bond;Extracellular matrix;Glycoprotein;Lipoprotein;Reference proteome;Secreted;Signal;Wnt signaling pathway SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix Secreted " Q93212,PROTEIN NAMES: Suppressor of lurcher protein 1 ORGANISM: Caenorhabditis elegans FUNCTION: Accessory protein required for glutamate-gated currents. May participate in the gating of non-NMDA (N-methyl-D-aspartate) ionotropic glutamate receptors such as glr-1. KEYWORDS: Cell membrane;Disulfide bond;Glycoprotein;Membrane;Postsynaptic cell membrane;Reference proteome;Repeat;Signal;Synapse;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Postsynaptic cell membrane ; Single-pass type I membrane protein Synapse Note=Colocalizes with glr-1 at the cell surface. Enriched at postsynaptic membranes. Q93246,"PROTEIN NAMES: Homeobox protein dsc-1 (Defecation suppressor of clk-1) ORGANISM: Caenorhabditis elegans FUNCTION: Transcriptional regulator which plays a role in the expulsion step of defecation by controlling enteric muscle-specific expression of exp-1 which is required for enteric muscle contraction. Not required for exp-1 expression in the PDA neuron. Also involved in controlling the length of the defecation cycle. KEYWORDS: Cell projection;Cytoplasm;Differentiation;DNA-binding;Homeobox;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus Cell projection, axon Cytoplasm " Q93367,"PROTEIN NAMES: Messenger RNA-binding inhibitor of apoptosis 1 ORGANISM: Caenorhabditis elegans FUNCTION: RNA-binding protein which binds to its own mRNA and target mRNAs to negatively regulate gene expression to modulate apoptosis and differentiation in the germline. Negatively regulates the expression of the argonaute protein wago-4, and may thus play a role in RNA-mediated gene silencing (RNAi) in the germline. KEYWORDS: 3D-structure;Cytoplasm;Reference proteome;RNA-binding SUBCELLULAR LOCATION: Cytoplasm, perinuclear region " Q93373,"PROTEIN NAMES: Leucine-rich repeat-containing protein let-4 (Lethal protein 4) ORGANISM: Caenorhabditis elegans FUNCTION: Required for apical extracellular matrix organization and epithelial junction maintenance. KEYWORDS: Cell membrane;Leucine-rich repeat;Membrane;Reference proteome;Repeat;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Apical cell membrane ; Single-pass type I membrane protein Note=In the excretory canal cell at the 1.5-fold embryonic stage, localized uniformly at the cell membrane. " Q93571,PROTEIN NAMES: Ubiquitin-conjugating enzyme E2 25 (E2 ubiquitin-conjugating enzyme 25) PROTEIN FAMILY: Ubiquitin-conjugating enzyme family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the ubiquitin-conjugating enzyme family. FUNCTION: Catalyzes the covalent attachment of ubiquitin to other proteins (Potential). Required for the maintenance of neuromuscular function. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: ATP-binding;Cytoplasm;Nucleotide-binding;Nucleus;Reference proteome;Transferase;Ubl conjugation pathway SUBCELLULAR LOCATION: Cytoplasm Nucleus Q93796,"PROTEIN NAMES: Nose resistant to fluoxetine protein 5 (Protein nrf-5) PROTEIN FAMILY: BPI/LBP/Plunc superfamily, BPI/LBP family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the BPI/LBP/Plunc superfamily. BPI/LBP family. FUNCTION: Plays a role in the uptake of a range of molecules including phosphatidylserine, lipids and xenobiotic compounds from the intestine to surrounding tissues. Possesses lipid transfer activity. Mediates transport of lipids from intestine to reproductive tract. Binds phosphatidylserine. Plays a role in efficient clearance of cell corpses by mediating phosphatidylserine appearance on phagocytic cells, thus promoting phagocytic engulfment of apoptotic cells. Vital for embryonic development. KEYWORDS: Developmental protein;Disulfide bond;Lipid transport;Lipid-binding;Reference proteome;Secreted;Signal;Transport SUBCELLULAR LOCATION: Secreted " Q93890,"PROTEIN NAMES: UDP-galactose/UDP-N-acetylglucosamine transporter srf-3 (Surface antigenicity abnormal 3) PROTEIN FAMILY: Nucleotide-sugar transporter family, SLC35A subfamily ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the nucleotide-sugar transporter family. SLC35A subfamily. FUNCTION: Acts as a transporter of both UDP-galactose and UDP-N-acetylglucosamine into the Golgi lumen. Apparently transports UDP-galactose and UDP-N-acetylglucosamine simultaneously, and independently, by an unknown mechanism. Functions redundantly with nucleotide sugar transporter nstp-4. May be involved in gonadal development. KEYWORDS: Golgi apparatus;Membrane;Reference proteome;Sugar transport;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Golgi apparatus membrane ; Multi-pass membrane protein " Q93LQ8,"PROTEIN NAMES: Beta-glucoside kinase PROTEIN FAMILY: ROK (NagC/XylR) family ORGANISM: Klebsiella pneumoniae SIMILARITY: Belongs to the ROK (NagC/XylR) family. FUNCTION: Catalyzes the ATP-dependent phosphorylation of a wide variety of beta-D-glucosides, to produce 6-phospho-beta-D-glucosides including cellobiose-6'-P, gentiobiose-6'-P, cellobiitol-6-P, salicin-6-P, and arbutin-6-P. Is not able to phosphorylate alpha-D-glucosides. May have a dual role of kinase and transcriptional regulator of the cellobiose-PTS operon. KEYWORDS: ATP-binding;Carbohydrate metabolism;Direct protein sequencing;Kinase;Nucleotide-binding;Transferase " Q93NG3,"PROTEIN NAMES: 2,6-dihydroxypyridine 3-monooxygenase (2,6-DHPH) ORGANISM: Paenarthrobacter nicotinovorans (Arthrobacter nicotinovorans) FUNCTION: Catalyzes the conversion of 2,6-dihydroxypyridine into 2,3,6-trihydroxypyridine in the nicotine degradation pathway. PATHWAY: Alkaloid degradation; nicotine degradation. KEYWORDS: 3D-structure;FAD;Flavoprotein;NAD;Nucleotide-binding;Oxidoreductase;Plasmid " Q93P60,"PROTEIN NAMES: Alpha-monoglucosyldiacylglycerol synthase (Alpha-MGS) (MGlcDAG synthase) (1,2-Diacylglycerol 3-glucosyltransferase) (UDP-glucose:1,2-diacylglycerol 3-alpha-D-glucosyltransferase) PROTEIN FAMILY: Glycosyltransferase group 1 family, Glycosyltransferase 4 subfamily ORGANISM: Acholeplasma laidlawii SIMILARITY: Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 4 subfamily. FUNCTION: Glucosyltransferase involved in the biosynthesis of the non-bilayer-prone membrane lipid alpha-monoglucosyldiacylglycerol. This is a major component for maintaining a certain anionic lipid surface charge density, for balancing the bilayer to non-bilayer phase equilibria and for keeping a constant lipid bilayer spontaneous curvature (curvature packing stress). Catalyzes the transfer of a glucosyl residue from UDP-Glc to diacylglycerol (DAG) acceptor to form the corresponding alpha-glucosyl-DAG (1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol). It can only use UDP-Glc as sugar donor and DAG is the preferred substrate. KEYWORDS: 3D-structure;Carbohydrate metabolism;Cell membrane;Direct protein sequencing;Glycerol metabolism;Glycosyltransferase;Lipid biosynthesis;Lipid metabolism;Magnesium;Membrane;Transferase SUBCELLULAR LOCATION: Cell membrane ; Peripheral membrane protein ; Cytoplasmic side Note=The enzyme binds the membrane surface via electrostatic association with anionic lipids and is accompanied by hydrophobic interactions and by a conformational change induced by nonbilayer-prone lipids. The enzyme is supposed to be located on the cytosolic side of the membrane. MISCELLANEOUS: PG could also protect the enzyme from proteolytic digestion of the proteinase K, possibly by orienting a lysine-rich face toward the membrane." Q93QX0,PROTEIN NAMES: Bifunctional aspartate aminotransferase and L-aspartate beta-decarboxylase (Aspartate 4-decarboxylase) (ASD) (AsdA) PROTEIN FAMILY: Class-I pyridoxal-phosphate-dependent aminotransferase family ORGANISM: Comamonas testosteroni (Pseudomonas testosteroni) SIMILARITY: Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. FUNCTION: Bifunctional enzyme that has both L-aspartate decarboxylase and transaminase activity. KEYWORDS: 3D-structure;Amino-acid biosynthesis;Aminotransferase;Decarboxylase;Lyase;Pyridoxal phosphate;Transferase Q93TJ5,"PROTEIN NAMES: 4-hydroxyacetophenone monooxygenase (HAPMO) (Baeyer-Villiger monooxygenase) (BVMO) PROTEIN FAMILY: FAD-binding monooxygenase family ORGANISM: Pseudomonas fluorescens SIMILARITY: Belongs to the FAD-binding monooxygenase family. FUNCTION: Catalyzes a Baeyer-Villiger oxidation reaction, i.e. the insertion of an oxygen atom into a carbon-carbon bond adjacent to a carbonyl, which converts ketones to esters. Can oxidize a wide range of acetophenone derivatives. Highest activity occurs with compounds bearing an electron-donating substituent at the para position of the aromatic ring, e.g. 4-hydroxyacetophenone and 4-aminoacetophenone, leading to the formation of 4-hydroxyphenyl acetate and 4-aminophenyl acetate, respectively. Is also able to oxidize sulfides. KEYWORDS: Direct protein sequencing;FAD;Flavoprotein;Monooxygenase;NADP;Oxidoreductase " Q93W54,"PROTEIN NAMES: Protein-S-isoprenylcysteine O-methyltransferase B (AtICMTB) (Isoprenylcysteine carboxylmethyltransferase B) (Prenylated protein carboxyl methyltransferase B) (Prenylcysteine carboxyl methyltransferase 14 B) (AtSTE14B) PROTEIN FAMILY: Class VI-like SAM-binding methyltransferase superfamily, Isoprenylcysteine carboxyl methyltransferase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the class VI-like SAM-binding methyltransferase superfamily. Isoprenylcysteine carboxyl methyltransferase family. FUNCTION: Catalyzes the post-translational methylation of isoprenylated C-terminal cysteine residues, resulting in the modulation of the function of prenylated proteins. Involved in negative regulation of abscisic acid signaling. Carboxyl methylation is a reversible and potentially regulated step in the post-translational modification of prenylated proteins. KEYWORDS: Endoplasmic reticulum;Membrane;Methyltransferase;Reference proteome;S-adenosyl-L-methionine;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein MISCELLANEOUS: ICMTB is more widely expressed and has a higher catalytic activity than ICMTA." Q93W95,"PROTEIN NAMES: Probable pectin methyltransferase QUA3 (Protein QUASIMODO 3) PROTEIN FAMILY: Methyltransferase superfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the methyltransferase superfamily. FUNCTION: S-adenosyl-L-methionine (SAM)-dependent methyltransferase (MTase) which mediates the methylesterification of the pectin homogalacturonan (HG) and thus regulates cell wall biosynthesis, at least in suspension-cultured cells. PATHWAY: Glycan metabolism; pectin biosynthesis. KEYWORDS: Glycoprotein;Golgi apparatus;Membrane;Methyltransferase;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus membrane ; Single-pass type II membrane protein " Q93WC9,"PROTEIN NAMES: Adenylate isopentenyltransferase 3, chloroplastic (AtIPT3) (Adenylate dimethylallyltransferase 3) (Cytokinin synthase 3) PROTEIN FAMILY: IPP transferase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the IPP transferase family. FUNCTION: Involved in cytokinin biosynthesis. Catalyzes the transfer of an isopentenyl group from dimethylallyl diphosphate (DMAPP) to ATP and ADP. KEYWORDS: ATP-binding;Chloroplast;Cytokinin biosynthesis;Cytoplasm;Lipoprotein;Membrane;Methylation;Nucleotide-binding;Nucleus;Plastid;Prenylation;Reference proteome;Transferase;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast Nucleus membrane ; Lipid-anchor Cytoplasm Note=Farnesylation directs most of the protein to the nucleus/cytoplasm despite the presence of a chloroplast transit peptide. " Q93WE4,"PROTEIN NAMES: Probable inactive E3 ubiquitin-protein ligase SINAT6 (Protein SEVEN IN ABSENTIA 2) (Seven in absentia homolog 6) PROTEIN FAMILY: SINA (Seven in absentia) family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the SINA (Seven in absentia) family. FUNCTION: Probable inactive E3 ubiquitin-protein ligase that plays a role in regulation of autophagy. Upon starvation, involved in maintaining ATG6 homeostasis by competitively associating with ATG6, a component of the autophagosome complex. Acts as a positive regulator of drought stress response. Functions as a positive regulator of abscisic acid-mediated stomatal closure. KEYWORDS: Cytoplasm;Metal-binding;Nucleus;Reference proteome;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cytoplasm Nucleus " Q93WL3,"PROTEIN NAMES: ATP-dependent Clp protease ATP-binding subunit CLPT1, chloroplastic (nClpC-like protein) PROTEIN FAMILY: ClpA/ClpB family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the ClpA/ClpB family. FUNCTION: Accessory protein regulating the assembly of the plastidial Clp protease system. CLPT1 first binds to the heptameric P-ring containing the CLP3-6 subunits followed by CLPT2, and only then does the P-ring combine with the R-ring composed of the clpP1 and CLPR1-4 subunits. Once the core complex is fully assembled, it then associates to the CLPC chaperone partner to form the functional protease. CLPT1 and CLPT2 are partially redundant. KEYWORDS: 3D-structure;Chloroplast;Plastid;Reference proteome;Repeat;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast " Q93WP4,"PROTEIN NAMES: Phosphoenolpyruvate phosphatase (PEP phosphatase) PROTEIN FAMILY: Metallophosphoesterase superfamily, Purple acid phosphatase family ORGANISM: Allium cepa (Onion) SIMILARITY: Belongs to the metallophosphoesterase superfamily. Purple acid phosphatase family. FUNCTION: Phosphoenolpyruvate phosphatase that probably operates in the vacuole to release phosphate from phosphoenolpyruvate (PEP) under phosphorus starvation. KEYWORDS: Glycoprotein;Hydrolase;Iron;Metal-binding;Protein phosphatase;Signal;Vacuole;Zinc SUBCELLULAR LOCATION: Vacuole lumen " Q93WU8,"PROTEIN NAMES: Probable WRKY transcription factor 54 (WRKY DNA-binding protein 54) PROTEIN FAMILY: WRKY group III family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the WRKY group III family. FUNCTION: Transcription factor. Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element (By similarity). Together with WRKY70, negative regulator of developmental senescence, probably via the regulation of several senescence-associated markers genes. Positive regulator of EDS1-dependent defense against E.amylovora. In collaboration with WRKY70, prevents stomatal closure and, consequently, osmotic stress tolerance. Together with WRKY46 and WRKY70, promotes brassinosteroid (BR)-regulated plant growth but prevent drought response by modulating gene expression. Negative regulator of SA biosynthesis. Prevents defense response to the necrotrophic pathogens P.carotovorum and B.cinerea, but promotes defense against biotrophic/hemibiotrophic pathogens P.syringae pv. tomato (Pst) DC3000, probably by regulating negatively the jasmonic acid (JA)/ethylene (ET) and positively the salicylic acid (SA) signaling pathways. KEYWORDS: Brassinosteroid signaling pathway;DNA-binding;Ethylene signaling pathway;Jasmonic acid signaling pathway;Nucleus;Phosphoprotein;Plant defense;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " Q93Y37,"PROTEIN NAMES: Mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS3 PROTEIN FAMILY: Glycosyl hydrolase 47 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the glycosyl hydrolase 47 family. FUNCTION: Class I alpha-mannosidase essential for early N-glycan processing. Removes preferentially alpha-1,2-linked mannose residues from Man(9)GlcNAc(2) to produce Man(8)GlcNAc(2). Involved in root development and cell wall biosynthesis. PATHWAY: Protein modification; protein glycosylation. KEYWORDS: Calcium;Disulfide bond;Glycoprotein;Golgi apparatus;Hydrolase;Magnesium;Manganese;Membrane;Metal-binding;Reference proteome;Signal-anchor;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus, cis-Golgi network membrane ; Single-pass type II membrane protein " Q93YN9,"PROTEIN NAMES: Inositol-pentakisphosphate 2-kinase (Inositol-1,3,4,5,6-pentakisphosphate 2-kinase) (Ins(1,3,4,5,6)P5 2-kinase) (AtIPK1) (InsP5 2-kinase) PROTEIN FAMILY: IPK1 type 2 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the IPK1 type 2 family. FUNCTION: Phosphorylates Ins(1,3,4,5,6)P5 at position 2 to form Ins(1,2,3,4,5,6)P6 (InsP6 or phytate). Phytate is a regulator of intracellular signaling, a highly abundant animal antinutrient, and a phosphate store in plant seeds. Also phosphorylates Ins(1,3,4,6)P4 and Ins(1,4,5,6)P4 to produce Ins(1,2,3,4,6)P5 and Ins(1,2,4,5,6)P5. KEYWORDS: 3D-structure;Acetylation;ATP-binding;Kinase;Metal-binding;Nucleotide-binding;Reference proteome;Transferase;Zinc " Q93YV0,"PROTEIN NAMES: (E,E)-geranyllinalool synthase (AtGES) (Terpenoid synthase 4) (AtTPS04) PROTEIN FAMILY: Terpene synthase family, Tpsf subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the terpene synthase family. Tpsf subfamily. FUNCTION: Involved in the biosynthesis of homoterpenes, attractants of herbivores parasitoids and predators (e.g. predatory mites and parasitoid wasps). Involved in diterpene (C20) biosynthesis. Catalyzes the conversion of geranylgeranyl diphosphate to (E,E)-geranyllinalool, the precursor of the insect-induced volatile C16-homoterpene TMTT. PATHWAY: Secondary metabolite biosynthesis; terpenoid biosynthesis. KEYWORDS: Cytoplasm;Lyase;Magnesium;Manganese;Metal-binding;Reference proteome SUBCELLULAR LOCATION: Cytoplasm " Q93YZ7,"PROTEIN NAMES: Acetolactate synthase small subunit 1, chloroplastic (ALS-interacting protein 1) (Acetohydroxyacid synthase small subunit 1) PROTEIN FAMILY: Acetolactate synthase small subunit family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the acetolactate synthase small subunit family. FUNCTION: Regulatory subunit of acetohydroxy-acid synthase. Involved in the feed-back inhibition by branched-chain amino acids. Contains 2 repeats, each of them being able to activate partially the catalytic subunit. The enzyme reconstituted with the first repeat is inhibited by leucine, but not by valine or isoleucine and the enzyme reconstituted with the second repeat is not inhibited by any branched-chain amino acid. In vitro, inhibited by valine and leucine, but not isoleucine. Involved in valine and leucine homeostasis. Required for reproductive development and sodium homeostasis. May play a role under prolonged flooding or oxygen deficiency. PATHWAY: Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 1/4.; PATHWAY: Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 1/4. KEYWORDS: 3D-structure;Alternative splicing;Amino-acid biosynthesis;Branched-chain amino acid biosynthesis;Chloroplast;Peroxisome;Plastid;Reference proteome;Repeat;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast Peroxisome MISCELLANEOUS: Plants overexpressing AHASS1 exhibit tolerance to low-oxygen stress." Q93ZV7,"PROTEIN NAMES: La protein 1 (AtLa1) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Binds to the 3' poly(U) terminus of nascent RNA polymerase III transcripts, protecting them from exonuclease digestion and facilitating their folding and maturation. KEYWORDS: Alternative splicing;Nucleus;Reference proteome;RNA-binding SUBCELLULAR LOCATION: Nucleus, nucleoplasm Nucleus, nucleolus " Q940D0,"PROTEIN NAMES: Two-component response regulator ARR1 PROTEIN FAMILY: ARR family, Type-B subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the ARR family. Type-B subfamily. FUNCTION: Transcriptional activator that binds specifically to the DNA sequence 5'-[AG]GATT-3'. Functions as a response regulator involved in His-to-Asp phosphorelay signal transduction system. Phosphorylation of the Asp residue in the receiver domain activates the ability of the protein to promote the transcription of target genes. Could directly activate some type-A response regulators in response to cytokinins. Regulates SHY2 by binding to its promoter. Involved in the root-meristem size determination through the regulation of cell differentiation. KEYWORDS: 3D-structure;Activator;Alternative splicing;Cytokinin signaling pathway;DNA-binding;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation;Two-component regulatory system SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: [Isoform 2]: May be due to an intron retention." Q940E8,"PROTEIN NAMES: Leucine-rich repeat receptor-like protein FASCIATED EAR2 (CLAVATA2-like protein) ORGANISM: Zea mays (Maize) FUNCTION: Receptor-like protein that regulates shoot meristem proliferation. Based on additive and synergistic phenotypes of double mutants, it is probable that unlike CLV1 and CLV2 in A.thaliana, FAE2 and TD1 do not function exclusively in a single pathway. KEYWORDS: Cell membrane;Developmental protein;Differentiation;Glycoprotein;Leucine-rich repeat;Membrane;Receptor;Reference proteome;Repeat;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein " Q940G0,PROTEIN NAMES: Transmembrane 9 superfamily member 1 (Endomembrane protein 12) (Transmembrane nine protein 1) (AtTMN1) PROTEIN FAMILY: Nonaspanin (TM9SF) family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the nonaspanin (TM9SF) (TC 9.A.2) family. KEYWORDS: Endosome;Golgi apparatus;Membrane;Reference proteome;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endosome membrane ; Multi-pass membrane protein Golgi apparatus membrane ; Multi-pass membrane protein Q941I6,"PROTEIN NAMES: DNA mismatch repair protein PMS1 (Postmeiotic segregation protein 1) (Protein POSTMEIOTIC SEGREGATION 1) PROTEIN FAMILY: DNA mismatch repair MutL/HexB family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the DNA mismatch repair MutL/HexB family. FUNCTION: Required for DNA mismatch repair (MMR), correcting base-base mismatches and insertion-deletion loops (IDLs) resulting from DNA replication, DNA damage or from recombination events between non-identical sequences during meiosis. Component of the MutLalpha heterodimer that forms a ternary complex with the MutS heterodimers, which initially recognize the DNA mismatches. This complex is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. Plays a major role in maintaining the genetic stability of simple sequence repeats and in the repair of heteroduplex sites present in meiotic recombination intermediates. Does not seem to be required for homologous somatic recombination. KEYWORDS: Alternative splicing;DNA damage;DNA repair;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: [Isoform 2]: May be due to a competing acceptor splice site and an intron retention." Q941T1,"PROTEIN NAMES: Delta-1-pyrroline-5-carboxylate synthase 2 (OsP5CS2) [Includes: Glutamate 5-kinase (GK) (Gamma-glutamyl kinase); Gamma-glutamyl phosphate reductase (GPR) (Glutamate-5-semialdehyde dehydrogenase) (Glutamyl-gamma-semialdehyde dehydrogenase)] PROTEIN FAMILY: Glutamate 5-kinase family; Gamma-glutamyl phosphate reductase family ORGANISM: Oryza sativa subsp. japonica (Rice) SIMILARITY: In the N-terminal section; belongs to the glutamate 5-kinase family.; SIMILARITY: In the C-terminal section; belongs to the gamma-glutamyl phosphate reductase family. FUNCTION: P5CS plays a key role in proline biosynthesis, leading to osmoregulation in plants. Involved in abiotic stress tolerance. PATHWAY: Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2.; PATHWAY: Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2. KEYWORDS: Amino-acid biosynthesis;ATP-binding;Kinase;Multifunctional enzyme;NADP;Nucleotide-binding;Oxidoreductase;Proline biosynthesis;Reference proteome;Stress response;Transferase MISCELLANEOUS: Tobacco plants over-expressing P5CS1 and P5CS2 have elevated proline levels and display enhanced abiotic stress tolerance." Q94252,"PROTEIN NAMES: Lipase lipl-4 (Lipase-like 4) PROTEIN FAMILY: AB hydrolase superfamily, Lipase family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the AB hydrolase superfamily. Lipase family. FUNCTION: Lysosomal lipase that regulates the metabolism of long-chain fatty acids, primarily in response to nutrient availability. The production of these lipid signaling mediators regulates various processes, including lipolysis, autophagy, mitochondrial beta-oxidation, which in turn control lifespan and adaptation to starvation. In response to fasting, promotes the production of omega-6 polyunsaturated fatty acids (PUFAs), which in turn activates autophagy resulting in lifespan extension. Produces oleoylethanolamide (OEA), a ligand for the lysosomal lipid chaperone lbp-8 which translocates into the nucleus where it activates the transcription of genes promoting longevity and activation of mitochondrial beta oxidation. KEYWORDS: Alternative splicing;Glycoprotein;Hydrolase;Lipid degradation;Lipid metabolism;Lysosome;Reference proteome;Signal SUBCELLULAR LOCATION: Lysosome lumen " Q94361,"PROTEIN NAMES: E3 SUMO-protein ligase gei-17 (E3 SUMO-protein transferase gei-17) (Gex-3-interacting protein 17) PROTEIN FAMILY: PIAS family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the PIAS family. FUNCTION: Functions as an E3-type smo-1 ligase. Mediates smo-1 conjugation to air-2 in vitro and is required for proper chromosome alignment. In the early embryo, specifically suppresses checkpoint activation in response to DNA damage, maybe by promoting mus-101 sumoylation. In embryos, plays a role in determining telomere localization in the nucleus. PATHWAY: Protein modification; protein sumoylation. KEYWORDS: Alternative splicing;DNA damage;DNA repair;Metal-binding;Reference proteome;Transferase;Ubl conjugation pathway;Zinc;Zinc-finger " Q94420,"PROTEIN NAMES: Protein maternal effect lethal 26 ORGANISM: Caenorhabditis elegans FUNCTION: Probable substrate-specific adapter of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Controls degradation of microtubule severing protein mei-1 after meiosis. Controls degradation of ppfr-1, the regulatory subunit of PP4 complex, after meiosis. In body wall muscles, involved in the organization of myosin thick filaments, likely by regulating the degradation of mei-1 downstream of unc-89. May also activate the TORC1 pathway. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Cytoplasm;Reference proteome;Ubl conjugation pathway SUBCELLULAR LOCATION: Cytoplasm, myofibril, sarcomere, M line Cytoplasm, myofibril, sarcomere, I band Note=Colocalizes with unc-89 to the M line. " Q944G5,"PROTEIN NAMES: Protein NRT1/ PTR FAMILY 2.10 (AtNPF2.10) (Protein GLUCOSINOLATE TRANSPORTER-1) PROTEIN FAMILY: Major facilitator superfamily, Proton-dependent oligopeptide transporter (POT/PTR) family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the major facilitator superfamily. Proton-dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family. FUNCTION: High-affinity, proton-dependent glucosinolate-specific transporter. Involved in the distribution of glucosinolates within the leaf, including import into the glucosinolate-rich S-cells located adjacent to the phloem. Involved in bidirectional long-distance transport of aliphatic but not indole glucosinolates. May be involved in removal of glucosinolates from the xylem in roots. KEYWORDS: Cell membrane;Membrane;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein " Q94502,"PROTEIN NAMES: Neutral alpha-glucosidase AB (Alpha-glucosidase 2) (Glucosidase II subunit alpha) (Protein post-translational modification mutant A) PROTEIN FAMILY: Glycosyl hydrolase 31 family ORGANISM: Dictyostelium discoideum (Social amoeba) SIMILARITY: Belongs to the glycosyl hydrolase 31 family. FUNCTION: Cleaves sequentially the 2 innermost alpha-1,3-linked glucose residues from N-linked oligosaccharides on newly synthesized glycoproteins. PATHWAY: Glycan metabolism; N-glycan metabolism. KEYWORDS: Endoplasmic reticulum;Glycoprotein;Glycosidase;Golgi apparatus;Hydrolase;Reference proteome;Signal SUBCELLULAR LOCATION: Endoplasmic reticulum Golgi apparatus " Q94521,"PROTEIN NAMES: Arylalkylamine N-acetyltransferase 1 (Dopamine N-acetyltransferase) PROTEIN FAMILY: Acetyltransferase family, AANAT subfamily ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the acetyltransferase family. AANAT subfamily. FUNCTION: Catalyzes N-acetylation of tryptamine, tyramine, dopamine, serotonin and octopamine. In astrocytes, regulates sleep homeostasis by limiting the accumulation of serotonin and dopamine in the brain upon sleep deprivation. Is not essential for sclerotization. PATHWAY: Aromatic compound metabolism; melatonin biosynthesis; melatonin from serotonin: step 1/2. KEYWORDS: 3D-structure;Acyltransferase;Alternative splicing;Cytoplasm;Direct protein sequencing;Melatonin biosynthesis;Nucleus;Reference proteome;Transferase SUBCELLULAR LOCATION: Cytoplasm Nucleus Note=In astrocytes, is primarily cytoplasmic, but in neurons is also nuclear. " Q94527,"PROTEIN NAMES: Nuclear factor NF-kappa-B p110 subunit (Rel-p110) (Relish protein) [Cleaved into: Nuclear factor NF-kappa-B p68 subunit (Rel-p68); Nuclear factor NF-kappa-B p49 subunit (Rel-p49)] ORGANISM: Drosophila melanogaster (Fruit fly) FUNCTION: Transcription factor that plays a key role in the humoral immune response as part of the peptidoglycan recognition protein (IMD) signaling pathway. Rel-p68 subunit translocates to the nucleus where it binds to the promoter of the Cecropin A1 gene and probably other antimicrobial peptide genes. I-kappa-B kinase complex (IKKbeta and key) and PGRP-LC are essential signaling components in transmitting the lipopolysaccharide (LPS) signal leading to cact degradation for NF-kappa-B (rel) activation. Part of a Toll-related receptor pathway that functions in the apoptosis of unfit cells during cell competition. Also part of some antiviral immunity: activated downstream of Sting signaling, which detects double-stranded RNA (dsRNA) from viruses, and promotes expression of antiviral effector genes. May be part of a NF-kappa-B and Tollo signaling cascade that regulates development of the peripheral nervous system. Possibly post-transcriptionally regulates the neuron-specific genes sc and ase, by promoting the rapid turnover of their transcripts in the wing imaginal disk. KEYWORDS: Activator;Alternative splicing;ANK repeat;Antiviral defense;Cytoplasm;Direct protein sequencing;Immunity;Innate immunity;Nucleus;Phosphoprotein;Reference proteome;Repeat;Transcription;Transcription regulation SUBCELLULAR LOCATION: [Nuclear factor NF-kappa-B p68 subunit]: Nucleus.; SUBCELLULAR LOCATION: [Nuclear factor NF-kappa-B p110 subunit]: Cytoplasm.; SUBCELLULAR LOCATION: [Nuclear factor NF-kappa-B p49 subunit]: Cytoplasm. " Q94545,"PROTEIN NAMES: Set1/Ash2 histone methyltransferase complex subunit ASH2 (Absent, small, or homeotic discs protein 2) ORGANISM: Drosophila melanogaster (Fruit fly) FUNCTION: Transcriptional regulator. Regulates a number of genes involved in wing development including activation of net and bs and repression of rho and kni and controls vein-intervein patterning during wing development. Required for correct expression of a number of homeotic genes including Scr in the first leg imaginal disk and Ubx in the third leg imaginal disk and haltere disks. Required for stabilization of the histone-lysine N-methyltransferase trr and for trimethylation of 'Lys-4' of histone H3. Together with sktl probably plays a role in maintenance of transcriptionally active chromatin through down-regulation of histone H1 hyperphosphorylation. KEYWORDS: Alternative splicing;Chromosome;Developmental protein;DNA-binding;Metal-binding;Nucleus;Reference proteome;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Chromosome Note=Accumulates on salivary gland polytene chromosomes. " Q946Y7,"PROTEIN NAMES: Syntaxin-61 (AtSYP61) (Osmotic stress-sensitive mutant 1) (Protein SYNTAXIN OF PLANTS 61) PROTEIN FAMILY: Syntaxin family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the syntaxin family. FUNCTION: Vesicle trafficking protein that functions in the secretory pathway; the fusion of phospholipid vesicles containing SYP61 and VTI12 is triggered by YKT61 and YKT62. Together with VTI12, required for membrane fusion. Involved in osmotic stress tolerance and in abscisic acid (ABA) regulation of stomatal responses. Plays a role in the exocytic trafficking of cellulose synthases (CESAs) and the transport of cell wall components to the plasma membrane. Together with SYP121, regulates the post-Golgi trafficking of the aquaporin PIP2-7 to the plasma membrane, thus modulating cell membrane water permeability. KEYWORDS: Abscisic acid signaling pathway;Alternative splicing;Direct protein sequencing;Golgi apparatus;Membrane;Protein transport;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Golgi apparatus, trans-Golgi network membrane ; Single-pass type IV membrane protein Prevacuolar compartment membrane ; Single-pass type IV membrane protein Note=And along the cell wall inner surface. Intracellular trafficking is modulated by TNO1. MISCELLANEOUS: The exact location of the SYP51/SYP61 complex is unclear, but is probably on the trans-Golgi network because of the likelihood of containing chiefly VTI12." Q94901,"PROTEIN NAMES: RNA-binding protein lark ORGANISM: Drosophila melanogaster (Fruit fly) FUNCTION: Essential RNA-binding protein. May be required for circadian repression of eclosion. Also essential for nurse cell dumping during oogenesis, the process whereby the cytoplasmic contents of nurse cells are transferred to the oocyte late in it's development. KEYWORDS: Biological rhythms;Cytoplasm;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Repeat;RNA-binding;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cytoplasm Nucleus Note=The precise location within neurons varies according to the type of neuron, being cytoplasmic in CCAP neurons and nuclear in the CNS. " Q94918,"PROTEIN NAMES: Protein vein (Epidermal growth factor-like protein) (Protein defective dorsal discs) ORGANISM: Drosophila melanogaster (Fruit fly) FUNCTION: Ligand for the EGF receptor. Seems to play a role in the global proliferation of wing disc cells and the larval patterning. Shows a strong synergistic genetic interaction with spi, suggesting a molecular interdependence. Required for the development of interveins cells. KEYWORDS: Alternative splicing;Developmental protein;Disulfide bond;EGF-like domain;Glycoprotein;Growth factor;Immunoglobulin domain;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted. " Q949T8,"PROTEIN NAMES: Histone-lysine N-methyltransferase ASHR3 (ASH1-related protein 3) (Protein SET DOMAIN GROUP 4) (Protein stamen loss) PROTEIN FAMILY: Class V-like SAM-binding methyltransferase superfamily, Histone-lysine methyltransferase family, SET2 subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. SET2 subfamily. FUNCTION: Histone methyltransferase (By similarity). Involved in stamen development. KEYWORDS: Chromatin regulator;Chromosome;Developmental protein;Metal-binding;Methyltransferase;Nucleus;Reference proteome;S-adenosyl-L-methionine;Transferase;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Chromosome Note=Associated to the euchromatin. " Q949U1,"PROTEIN NAMES: Dihomomethionine N-hydroxylase (Cytochrome P450 79F1) (Protein BUSHY 1) (Protein SUPERSHOOT 1) (Trihomomethionine N-hydroxylase) PROTEIN FAMILY: Cytochrome P450 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the cytochrome P450 family. FUNCTION: Catalyzes the conversion of the short chain elongated methionines di-, tri-, and tetrahomomethionine to their respective aldoximes 5-methylthiopentanaldoxime, 6-methylthiohexanaldoxime, and 7-methylheptanaldoxime. KEYWORDS: Alternative splicing;Endoplasmic reticulum;Heme;Iron;Membrane;Metal-binding;Monooxygenase;Oxidoreductase;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Single-pass membrane protein " Q949V6,"PROTEIN NAMES: Probable E3 ubiquitin-protein ligase ARI1 (ARIADNE-like protein ARI1) (Protein ariadne homolog 1) (RING-type E3 ubiquitin transferase ARI1) PROTEIN FAMILY: RBR family, Ariadne subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the RBR family. Ariadne subfamily. FUNCTION: Might act as an E3 ubiquitin-protein ligase, or as part of E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes and then transfers it to substrates. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Alternative splicing;Metal-binding;Reference proteome;Repeat;Transferase;Ubl conjugation pathway;Zinc;Zinc-finger " Q949W8,PROTEIN NAMES: Xylulose kinase 2 (Atxk-2) (Xylulokinase 2) (1-Deoxy-D-Xylulokinase) (DXK) PROTEIN FAMILY: FGGY kinase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the FGGY kinase family. FUNCTION: Mediates 1-deoxy-D-xylulose (DX) phosphorylation in the cytoplasm prior to the translocation of 1-deoxy-D-xylulose 5-phosphate into plastids. Can also phosphorylate D-xylulose (Xyl). Uses preferentially ATP as cosubstrate. PATHWAY: Isoprenoid biosynthesis; carotenoid biosynthesis. KEYWORDS: ATP-binding;Cytoplasm;Kinase;Nucleotide-binding;Reference proteome;Transferase SUBCELLULAR LOCATION: Cytoplasm Q949X0,"PROTEIN NAMES: Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic (Acyl-lipid desaturase 3) (Fatty acid desaturase 5) (FAD5) (Fatty acid desaturase B) (FADB) (Monogalactosyldiacylglycerol-specific palmitic acid desaturase) PROTEIN FAMILY: Fatty acid desaturase type 1 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the fatty acid desaturase type 1 family. FUNCTION: Fatty acid desaturase involved in the first desaturation step leading to the formation of hexadeca 7,10,13-trienoic acid (16:3(7Z,10Z,13Z)), the major functional components of thylakoid membranes. Required for chloroplast biogenesis at low temperature. Also indirectly involved in the production of the oxylipin dinor-oxo-phyto-dienoic acid implicated in wound signaling. PATHWAY: Lipid metabolism; oxylipin biosynthesis.; PATHWAY: Lipid metabolism; polyunsaturated fatty acid biosynthesis. KEYWORDS: Chloroplast;Fatty acid biosynthesis;Fatty acid metabolism;Iron;Lipid biosynthesis;Lipid metabolism;Membrane;Oxidoreductase;Oxylipin biosynthesis;Plastid;Reference proteome;Transit peptide;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Plastid, chloroplast membrane ; Multi-pass membrane protein MISCELLANEOUS: Substrate specificity shifts from delta-7 to delta-9 desaturation when the protein is retargeted to the cytoplasm." Q949Y0,PROTEIN NAMES: Ubiquitin carboxyl-terminal hydrolase 6 (Deubiquitinating enzyme 6) (AtUBP6) (Ubiquitin thioesterase 6) (Ubiquitin-specific-processing protease 6) PROTEIN FAMILY: Peptidase C19 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the peptidase C19 family. FUNCTION: Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins. KEYWORDS: Alternative splicing;Calmodulin-binding;Hydrolase;Protease;Reference proteome;Thiol protease;Ubl conjugation pathway Q94A57,PROTEIN NAMES: Protein PHR1-LIKE 2 (Myb family transcription factor PHL2) PROTEIN FAMILY: MYB-CC family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the MYB-CC family. FUNCTION: Transcriptional activator. Acts redundantly with PHR1 as a key component of the central regulatory system controlling transcriptional responses to Pi starvation. Binds in a sequence-specific manner to phosphate starvation-regulated promoters. KEYWORDS: Alternative splicing;Coiled coil;DNA-binding;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus Q94A82,"PROTEIN NAMES: Nudix hydrolase 19, chloroplastic (AtNUDT19) (NAD-capped RNA hydrolase NUDT19) (DeNADding enzyme NUDT19) (NADH pyrophosphatase NUDT19) PROTEIN FAMILY: Nudix hydrolase family, NudC subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the Nudix hydrolase family. NudC subfamily. FUNCTION: mRNA decapping enzyme that specifically removes the nicotinamide adenine dinucleotide (NAD) cap from a subset of mRNAs by hydrolyzing the diphosphate linkage to produce nicotinamide mononucleotide (NMN) and 5' monophosphate mRNA. The NAD-cap is present at the 5'-end of some RNAs; in contrast to the canonical N7 methylguanosine (m7G) cap, the NAD cap promotes mRNA decay (By similarity). Mediates the hydrolysis of some nucleoside diphosphate derivatives. Has a high affinity for NADPH compared with that for NADH. KEYWORDS: Chloroplast;Hydrolase;Magnesium;Metal-binding;NAD;Plastid;Reference proteome;Transit peptide;Zinc SUBCELLULAR LOCATION: Plastid, chloroplast " Q94A84,"PROTEIN NAMES: UDP-glycosyltransferase 72E1 PROTEIN FAMILY: UDP-glycosyltransferase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the UDP-glycosyltransferase family. FUNCTION: UDP-glycosyltransferase that glucosylates coniferyl aldehyde to form coniferyl aldehyde 4-O-glucoside. Glucosylates sinapyl aldehyde to form sinapyl aldehyde 4-O-glucoside. Is not active in presence of coniferyl alcohol or sinapyl alcohol. Can glucosylate the phytotoxic xenobiotic compound 2,4,5-trichlorophenol (TCP). KEYWORDS: Detoxification;Glycosyltransferase;Nucleus;Reference proteome;Transferase SUBCELLULAR LOCATION: Nucleus Note=Colocalizes with SIS8 in the nucleus. " Q94AA9,"PROTEIN NAMES: Xylogalacturonan beta-1,3-xylosyltransferase (Protein XYLOGALACTURONAN DEFICIENT 1) PROTEIN FAMILY: Glycosyltransferase 47 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the glycosyltransferase 47 family. FUNCTION: Involved in pectin biosynthesis. Catalyzes the transfer of xylose from UDP-xylose onto oligogalacturonides and endogenous acceptors. KEYWORDS: Cell wall biogenesis/degradation;Glycoprotein;Glycosyltransferase;Golgi apparatus;Membrane;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus membrane; Single-pass type II membrane protein. " Q94AH1,"PROTEIN NAMES: Protein CANDIDATE G-PROTEIN COUPLED RECEPTOR 2 (AtCand2) (Protein PHYTOMELATONIN RECEPTOR 1) PROTEIN FAMILY: UPF0359 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the UPF0359 family. FUNCTION: Plays a role in plants and microbes interactions. G-protein coupled melatonin receptor involved in root growth mediated by the bacterial quorum-sensing signals N-acyl-homoserine lactones (AHLs). Binds to melatonin. Phytomelatonin receptor required, in collaboration with GPA1, for melatonin-mediated stomatal closure involving H(2)O(2) and Ca(2+) signals. Essential for melatonin-mediated plant response to osmotic stress probably by activating reactive oxygen species (ROS) scavenging ability. KEYWORDS: Cell membrane;Developmental protein;G-protein coupled receptor;Membrane;Receptor;Reference proteome;Transducer;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein " Q94AI6,"PROTEIN NAMES: Exocyst complex component SEC6 (AtSec6) PROTEIN FAMILY: SEC6 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the SEC6 family. FUNCTION: Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane during regulated or polarized secretion. Involved in polarized cell growth and organ morphogenesis. During cytokinesis, involved in cell plate initiation, cell plate maturation and formation of new primary cell wall. KEYWORDS: Acetylation;Alternative splicing;Cell membrane;Cell wall;Cytoplasm;Cytoskeleton;Exocytosis;Membrane;Reference proteome;Secreted;Transport SUBCELLULAR LOCATION: Cytoplasm, cytosol Cell membrane Cytoplasm, cytoskeleton, phragmoplast Secreted, cell wall Secreted, extracellular exosome Note=During cytokinesis, localizes to the nascent cell plate and later to the cell plate insertion site and along the post-cytokinetic wall. Shuttles from the cytoplasm to the exocyst-positive organelle (EXPO) in the presence of EXO70E2. " Q94AQ8,"PROTEIN NAMES: Photosynthetic NDH subunit of subcomplex B 2, chloroplastic (Protein PnsB2) (NAD(P)H DEHYDROGENASE SUBUNIT 45) (NDH-DEPENDENT CYCLIC ELECTRON FLOW 2) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: NDH shuttles electrons from NAD(P)H:plastoquinone, via FMN and iron-sulfur (Fe-S) centers, to quinones in the photosynthetic chain and possibly in a chloroplast respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton translocation, and thus conserves the redox energy in a proton gradient. KEYWORDS: 3D-structure;Alternative splicing;Chloroplast;Membrane;Plastid;Reference proteome;Thylakoid;Transit peptide;Transport SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane ; Peripheral membrane protein " Q94AU9,"PROTEIN NAMES: Chaperonin-like RBCX protein 1, chloroplastic (AtRBCX1) PROTEIN FAMILY: RbcX family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the RbcX family. FUNCTION: Chaperone involved in RuBisCO assembly process. KEYWORDS: 3D-structure;Carbon dioxide fixation;Chaperone;Chloroplast;Direct protein sequencing;Photosynthesis;Plastid;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast Note=Associated with the thylakoid membranes. " Q94AX4,"PROTEIN NAMES: D-lactate dehydrogenase [cytochrome], mitochondrial (AtD-LDH) (D-lactate ferricytochrome C oxidoreductase) (Glycolate dehydrogenase) PROTEIN FAMILY: FAD-binding oxidoreductase/transferase type 4 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the FAD-binding oxidoreductase/transferase type 4 family. FUNCTION: Catalyzes the stereospecific oxidation of D-lactate to pyruvate. Involved in the detoxification of methylglyoxal and D-lactate, but probably not involved in the metabolization of glycolate. KEYWORDS: FAD;Flavoprotein;Mitochondrion;Oxidoreductase;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Mitochondrion " Q94B65,"PROTEIN NAMES: UDP-galactose/UDP-glucose transporter 7 (AtUTr7) PROTEIN FAMILY: TPT transporter family, UGnT subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the TPT transporter family. UGnT (TC 2.A.7.15) subfamily. FUNCTION: Nucleotide-sugar transporter that transports UDP-glucose and UDP-galactose. Plays a role in lateral root and root hair development. KEYWORDS: Alternative splicing;Golgi apparatus;Membrane;Reference proteome;Sugar transport;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Golgi apparatus membrane ; Multi-pass membrane protein " Q94BQ3,"PROTEIN NAMES: Protein ALTERED SEED GERMINATION 2 ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: May function as a substrate adapter for CUL4-DDB1 E3 ubiquitin-protein ligase complex (Probable). Negative regulator of fatty acid biosynthetic process and accumulation. Acts as an abscisic acid (ABA) negative regulator. Involved in responses to salt (NaCl) and osmotic (e.g. in response to mannitol and PEG) stresses. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Abscisic acid signaling pathway;Alternative splicing;Coiled coil;Cytoplasm;Lipoprotein;Nucleus;Prenylation;Reference proteome;Repeat;TPR repeat;WD repeat SUBCELLULAR LOCATION: Nucleus Cytoplasm, cytosol Note=Excluded from the nucleus when farnesylated at Cys-754. " Q94CG0,"PROTEIN NAMES: CBL-interacting serine/threonine-protein kinase 21 (SNF1-related kinase 3.4) (SOS2-like protein kinase PKS23) PROTEIN FAMILY: Protein kinase superfamily, CAMK Ser/Thr protein kinase family, SNF1 subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily. FUNCTION: CIPK serine-threonine protein kinases interact with CBL proteins. Binding of a CBL protein to the regulatory NAF domain of CIPK protein lead to the activation of the kinase in a calcium-dependent manner (By similarity). KEYWORDS: ATP-binding;Kinase;Manganese;Nucleotide-binding;Phosphoprotein;Reference proteome;Serine/threonine-protein kinase;Transferase " Q94CL6,"PROTEIN NAMES: Protein NAR1 (Nuclear architecture related 1) (Protein GROWTH AT DIFFERENT OXYGEN LEVELS INFLUENCES MORPHOGENESIS) ([FeFe]-hydrogenase-like protein GOLLUM) PROTEIN FAMILY: NARF family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the NARF family. FUNCTION: Essential component of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery. Required for the maturation of extramitochondrial Fe/S proteins (By similarity). Required for expression of the imprinted FWA gene, for seed development and is involved in the oxidative stress response in vegetative tissues. Involved in the regulation of cell size, ploidy and cell cycle progression. Required for growth under normoxic conditions and necessary for recovery after hypoxic treatment but its action is reactive oxygen species (ROS) independent. KEYWORDS: 4Fe-4S;Cytoplasm;Iron;Iron-sulfur;Metal-binding;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus Cytoplasm MISCELLANEOUS: Coordinates probably two (Fe-S) clusters with different magnetic properties. Knockdown mutants have a dwarf phenotype, but are indistinguishable from wild type under hypoxic conditions." Q94CL7,"PROTEIN NAMES: Protein PHOSPHATE STARVATION RESPONSE 1 (AtPHR1) PROTEIN FAMILY: MYB-CC family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the MYB-CC family. FUNCTION: Transcription factor involved in phosphate starvation signaling. Binds as a dimer to P1BS, an imperfect palindromic sequence 5'-GNATATNC-3', to promote the expression of inorganic phosphate (Pi) starvation-responsive genes. SPX1 is a competitive inhibitor of this DNA-binding. PHR1 binding to its targets is low Pi-dependent. Regulates the expression of miR399. Regulates the expression of IPS1 (At3g09922), a non-coding RNA that mimics the target of miR399 to block the cleavage of PHO2 under Pi-deficient conditions. Regulates lipid remodeling and triacylglycerol accumulation during phosphorus starvation. Required for the shoot-specific hypoxic response. Regulates FER1 expression upon phosphate starvation, linking iron and phosphate homeostasis. Contributes to the homeostasis of both sulfate and phosphate in plants under phosphate deficiency. Required for adaptation to high light and retaining functional photosynthesis during phosphate starvation. Involved in the coregulation of Zn and Pi homeostasis. KEYWORDS: 3D-structure;Coiled coil;DNA-binding;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation;Ubl conjugation SUBCELLULAR LOCATION: Nucleus Note=The localization to the nucleus is independent of the Pi status. " Q94F30,"PROTEIN NAMES: Ubiquitin-like-specific protease ESD4 (Protein EARLY IN SHORT DAYS 4) (AtESD4) PROTEIN FAMILY: Peptidase C48 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the peptidase C48 family. FUNCTION: Protease that catalyzes two essential functions in the SUMO pathway: processing of full-length SUMOs to their mature forms and deconjugation of SUMO from targeted proteins. Cleaves precursors of SUM1 and SUM2, but not of SUM3 or SUM5. Able to release SUM1 and SUM2 from conjugates, but unable to cleave SUM3. Acts predominantly as an isopeptidase, cleaving SUMO-conjugated proteins better than SUMO peptides. Plays an important role in the control of flowering time. KEYWORDS: Coiled coil;Hydrolase;Membrane;Nucleus;Protease;Reference proteome;Thiol protease;Ubl conjugation pathway SUBCELLULAR LOCATION: Nucleus membrane ; Peripheral membrane protein Note=The nuclear envelope localization is independent of the presence of the nuclear pore anchor NUA. " Q94F58,PROTEIN NAMES: NAC domain-containing protein 89 (ANAC089) (Protein FRUCTOSE-SENSING QUANTITATIVE TRAIT LOCUS 6) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Transcription factor involved in plant cell division. KEYWORDS: Cytoplasm;DNA-binding;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Cytoplasm Nucleus Note=Relocalization from the cytoplasm into the nucleus is induced by auxin treatment and in association with PAS1. Q94FL7,"PROTEIN NAMES: Transcription factor MYB120 (Myb-related protein 120) (AtMYB120) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Transcription activator. Binds to 5'-CAACTGTC-3' and/or 5'-TAACAAA-3' motif in target gene promoter to promote their expression (By similarity). Together with MYB97 and MYB101, functions as a male factor that controls pollen tube-synergid interaction in fertilization. Required for pollen tube growth arrest and sperm cell release in the female gametophyte, probably via the regulation of pollen tube-specific gene expression. KEYWORDS: Activator;Alternative splicing;DNA-binding;Nucleus;Reference proteome;Repeat;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus Note=Detected in the vegetative nucleus of pollen tubes. " Q94FL9,"PROTEIN NAMES: Transcription factor MYB3R-4 (Myb-related protein 3R-4) (Protein ENHANCED DEFECTIVE CYTOKINESIS) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Transcription factor that binds 5'-AACGG-3' motifs in gene promoters. Involved in the regulation of cytokinesis, probably via the activation of several G2/M phase-specific genes transcription (e.g. KNOLLE). Required for the maintenance of diploidy.; FUNCTION: Involved in transcription regulation during induced endoreduplication at the powdery mildew (e.g. G.orontii) infection site, thus promoting G.orontii growth and reproduction. KEYWORDS: Activator;Alternative splicing;DNA-binding;Nucleus;Reference proteome;Repeat;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " Q94FY7,"PROTEIN NAMES: Tocopherol cyclase, chloroplastic (Sucrose export defective 1) (Vitamin E pathway gene 1 protein) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Involved in the synthesis of both tocopherols and tocotrienols (vitamin E), which presumably protect photosynthetic complexes from oxidative stress. Catalyzes the conversion of 2-methyl-6-phytyl-1,4-hydroquinone and 2,3-dimethyl-5-phytyl-1,4-hydroquinone (DMPQ) to delta- and gamma-tocopherol respectively. Converts also 2,3-dimethyl-5-geranylgeranyl-1,4-hydroquinone (DMGQ) to gamma-tocotrienol. PATHWAY: Cofactor biosynthesis; tocopherol biosynthesis. KEYWORDS: Chloroplast;Isomerase;Plastid;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast, plastoglobule " Q94ID3,"PROTEIN NAMES: Adenylate isopentenyltransferase 1, chloroplastic (AtIPT1) (Adenylate dimethylallyltransferase 1) (Cytokinin synthase 1) PROTEIN FAMILY: IPP transferase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the IPP transferase family. FUNCTION: Involved in cytokinin biosynthesis. Catalyzes the transfer of an isopentenyl group from dimethylallyl diphosphate (DMAPP) to ATP, ADP and AMP. Adenine, adenosine, isopentenylpyrophosphate and 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate (HMBDP) are not used as substrates. KEYWORDS: ATP-binding;Chloroplast;Cytokinin biosynthesis;Nucleotide-binding;Plastid;Reference proteome;Transferase;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast " Q94IG7,"PROTEIN NAMES: Protoporphyrinogen oxidase 2 (Protox II) (SO-POX2) PROTEIN FAMILY: Protoporphyrinogen/coproporphyrinogen oxidase family, Protoporphyrinogen oxidase subfamily ORGANISM: Spinacia oleracea (Spinach) SIMILARITY: Belongs to the protoporphyrinogen/coproporphyrinogen oxidase family. Protoporphyrinogen oxidase subfamily. FUNCTION: Catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX.; FUNCTION: Provides precursor for the mitochondrial and plastidic heme synthesis and the predominant chlorophyll synthesis in plastids. PATHWAY: Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; protoporphyrin-IX from protoporphyrinogen-IX: step 1/1.; PATHWAY: Porphyrin-containing compound metabolism; chlorophyll biosynthesis. KEYWORDS: Alternative initiation;Chlorophyll biosynthesis;Chloroplast;FAD;Flavoprotein;Heme biosynthesis;Membrane;Mitochondrion;Mitochondrion inner membrane;Oxidoreductase;Plastid;Plastid inner membrane;Porphyrin biosynthesis;Reference proteome;Transit peptide SUBCELLULAR LOCATION: [Isoform L]: Plastid, chloroplast inner membrane ; Peripheral membrane protein ; Stromal side.; SUBCELLULAR LOCATION: [Isoform S]: Mitochondrion inner membrane ; Peripheral membrane protein MISCELLANEOUS: [Isoform S]: Produced by alternative initiation at Met-27 of isoform S. Mitochondrial precursor. Contains a mitochondrial transit peptide from 1 to 22 which is removed in mature form." Q94KD3,"PROTEIN NAMES: Vacuolar protein sorting-associated protein 52 A (AtVPS52) (ARE1-like protein POK) (Protein POKY POLLEN TUBE) (Protein T-DNA TRANSMISSION DEFECT 8) PROTEIN FAMILY: VPS52 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the VPS52 family. FUNCTION: Acts as a component of the GARP complex that is involved in retrograde transport from early and late endosomes to the trans-Golgi network (TGN). The GARP complex facilitates tethering as well as SNARE complex assembly at the Golgi (By similarity). Required for pollen tube elongation and other polar growth. KEYWORDS: Coiled coil;Endosome;Golgi apparatus;Membrane;Protein transport;Reference proteome;Transport SUBCELLULAR LOCATION: Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein. Endosome membrane; Peripheral membrane protein. Golgi apparatus membrane; Peripheral membrane protein. Note=Localized in the GARP complex in the Golgi and post-Golgi compartments. " Q95JH0,"PROTEIN NAMES: 1-acyl-sn-glycerol-3-phosphate acyltransferase alpha (1-acylglycerol-3-phosphate O-acyltransferase 1) (1-AGP acyltransferase 1) (1-AGPAT 1) (Lysophosphatidic acid acyltransferase alpha) (LPAAT-alpha) PROTEIN FAMILY: 1-acyl-sn-glycerol-3-phosphate acyltransferase family ORGANISM: Ovis aries (Sheep) SIMILARITY: Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family. FUNCTION: Converts 1-acyl-sn-glycerol-3-phosphate (lysophosphatidic acid or LPA) into 1,2-diacyl-sn-glycerol-3-phosphate (phosphatidic acid or PA) by incorporating an acyl moiety at the sn-2 position of the glycerol backbone. PATHWAY: Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 2/3. KEYWORDS: Acyltransferase;Endoplasmic reticulum;Lipid biosynthesis;Lipid metabolism;Membrane;Phospholipid biosynthesis;Phospholipid metabolism;Reference proteome;Signal;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein " Q95NH6,PROTEIN NAMES: Attacin-C [Cleaved into: Immune-induced peptide 16 (DIM-16) (DIM16) (MPAC)] PROTEIN FAMILY: Attacin/sarcotoxin-2 family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the attacin/sarcotoxin-2 family. FUNCTION: Has antimicrobial activity in synergy with other peptides. Strongest activity observed against E.cloacae. KEYWORDS: Antibiotic;Antimicrobial;Direct protein sequencing;Glycoprotein;Immunity;Innate immunity;Phosphoprotein;Pyrrolidone carboxylic acid;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted MISCELLANEOUS: Not induced by bacterial challenge in imd mutant flies. Level of induction reduced by over 90% in 18w mutant flies. Toll loss-of-function mutation has no effect on induction by a mixture of E.coli and M.luteus; but strongly reduces induction by E.cloacae. Q95NU5,"PROTEIN NAMES: Torsin-like protein (Abnormal oocyte formation protein 5) PROTEIN FAMILY: ClpA/ClpB family, Torsin subfamily ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the ClpA/ClpB family. Torsin subfamily. FUNCTION: May serve as a molecular chaperone assisting in the proper folding of secreted and/or membrane proteins. KEYWORDS: Alternative splicing;ATP-binding;Endoplasmic reticulum;Glycoprotein;Nucleotide-binding;Reference proteome;Signal SUBCELLULAR LOCATION: Endoplasmic reticulum lumen " Q95P23,PROTEIN NAMES: Enterin neuropeptides [Cleaved into: ENa; ENb; ENc; ENd; ENe; ENf; ENg; ENh; ENi; ENj; ENk; ENl; ENm; ENn; ENo; ENp; ENq; ENr; ENs; ENt] ORGANISM: Aplysia californica (California sea hare) FUNCTION: Reduce interneurons B4/5 activity. May play a regulatory role in nonfeeding behaviors. KEYWORDS: Amidation;Cleavage on pair of basic residues;Neuropeptide;Pyrrolidone carboxylic acid;Secreted;Signal SUBCELLULAR LOCATION: Secreted. Q95PZ9,"PROTEIN NAMES: Serine/threonine-protein kinase spe-6 (Defective spermatogenesis protein 6) PROTEIN FAMILY: Protein kinase superfamily, CK1 Ser/Thr protein kinase family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. FUNCTION: Serine/threonine-protein kinase which is involved in spermatogenesis. In spermatocytes, regulates meiosis and the localization and assembly of major sperm protein (MSP) into fibrous bodies. In addition, may suppress the initiation of spermiogenesis downstream of spe-8, spe-12, spe-27 and spe-29. KEYWORDS: ATP-binding;Differentiation;Kinase;Nucleotide-binding;Reference proteome;Serine/threonine-protein kinase;Spermatogenesis;Transferase " Q95Q34,"PROTEIN NAMES: Serine/threonine-protein kinase RIO2 PROTEIN FAMILY: Protein kinase superfamily, RIO-type Ser/Thr kinase family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the protein kinase superfamily. RIO-type Ser/Thr kinase family. FUNCTION: Required for larval development. KEYWORDS: ATP-binding;Kinase;Magnesium;Metal-binding;Nucleotide-binding;Reference proteome;Serine/threonine-protein kinase;Transferase " Q95QD7,PROTEIN NAMES: Protein lin-54 (Abnormal cell lineage protein 54) PROTEIN FAMILY: Lin-54 family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the lin-54 family. FUNCTION: Synthetic multivulva class B (synMuvB) protein. SynMuvB proteins are required to repress the induction of vulval development by Ras signaling and probably act by forming the multiprotein DRM complex that repress transcription. KEYWORDS: Alternative splicing;Cell cycle;Chromosome;DNA-binding;Metal-binding;Nucleus;Reference proteome;Repressor;Transcription;Transcription regulation;Zinc SUBCELLULAR LOCATION: Nucleus Chromosome Note=Localizes to condensed chromosomes during the diakinesis phase of meiosis. Q95RJ9,"PROTEIN NAMES: F-box-like/WD repeat-containing protein ebi PROTEIN FAMILY: WD repeat EBI family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the WD repeat EBI family. FUNCTION: F-box-like component of E3 ubiquitin ligase complexes; involved in R7 photoreceptor cell differentiation, cone cell development and neuronal cell cycle control. E3 ubiquitin ligase complexes mediate ubiquitination and subsequent proteasomal degradation of target proteins. Required for specification of R7 photoreceptor cell fate in the eye by participating in the ubiquitination and subsequent proteasomal degradation of Tramtrack (ttk), a general inhibitor of photoreceptor differentiation. Required to block the S phase entry in the peripheral nervous system and central nervous system in a process that does not involve the degradation of ttk. Involved in cone cell development by preventing the transcriptional repression mediated by Su(H) on Dl, probably by participating in a E3 complex that contains sno and mediates the ubiquitination and subsequent proteasomal degradation of some component of the Su(H) repressor complex. KEYWORDS: Cell cycle;Nucleus;Reference proteome;Repeat;Sensory transduction;Ubl conjugation pathway;Vision;WD repeat SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: 'Ebi' means 'shrimp' in Japanese." Q95RR8,"PROTEIN NAMES: PAN2-PAN3 deadenylation complex subunit PAN3 (PAB1P-dependent poly(A)-specific ribonuclease) (Poly(A)-nuclease deadenylation complex subunit 3) (PAN deadenylation complex subunit 3) PROTEIN FAMILY: Protein kinase superfamily, PAN3 family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the protein kinase superfamily. PAN3 family. FUNCTION: Regulatory subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in general and miRNA-mediated mRNA turnover. PAN specifically shortens poly(A) tails of RNA and the activity is stimulated by poly(A)-binding protein (PABP). PAN deadenylation is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex. Deadenylated mRNAs are then degraded by two alternative mechanisms, namely exosome-mediated 3'-5' exonucleolytic degradation, or deadenylation-dependent mRNA decaping and subsequent 5'-3' exonucleolytic degradation by XRN1. PAN3 acts as a positive regulator for PAN activity, recruiting the catalytic subunit PAN2 to mRNA via its interaction with RNA and PABP, and to miRNA targets via its interaction with GW182 family proteins. KEYWORDS: 3D-structure;ATP-binding;Coiled coil;Cytoplasm;mRNA processing;Nucleotide-binding;Reference proteome SUBCELLULAR LOCATION: Cytoplasm, P-body " Q95RV2,"PROTEIN NAMES: Telomere-binding protein cav (Anonymous fast evolving 1G5) (HP1/ORC-associated protein) (HOAP p55) (Protein caravaggio) ORGANISM: Drosophila melanogaster (Fruit fly) FUNCTION: Part of the HipHop-HOAP complex that recruits the MTV complex to form the terminin telomere-capping complex, which binds to chromosome ends in a sequence-independent manner and prevents telomere fusion. Telomere capping is independent of the origin recognition complex (ORC). KEYWORDS: Alternative splicing;Chromosome;Direct protein sequencing;DNA-binding;Nucleus;Reference proteome;Repeat;Telomere SUBCELLULAR LOCATION: Nucleus Chromosome Chromosome, telomere Note=Enriched at interphase, polytene and mitotic chromosome telomeres. Localized predominantly at telomeres and diffusely throughout regions of pericentric heterochromatin. Colocalizes with moi/modigliani at telomeres. Telomere localization is not dependent on peo/pendolino, tea, ver/verrocchio or moi/modigliani. Telomere localization is enhanced by, but not dependent on, Su(var)205/HP1. Telomere localization is enhanced by the activity of tefu/ATM and the MRN complex. MISCELLANEOUS: Multiple telomeric associations (TAs) in the same metaphase spread often result in multicentric linear chromosomes that resemble little 'trains' of chromosomes, hence the name 'caravaggio', the name of an Italian train named after the artist." Q95SX8,"PROTEIN NAMES: N-alpha-acetyltransferase 60 (dNaa60) (NatF catalytic subunit) PROTEIN FAMILY: Acetyltransferase family, NAA60 subfamily ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the acetyltransferase family. NAA60 subfamily. FUNCTION: Displays alpha (N-terminal) acetyltransferase activity towards a range of N-terminal sequences including those starting with Met-Lys, Met-Val, Met-Ala and Met-Met. Required for normal chromosomal segregation during anaphase.; FUNCTION: [Isoform A]: Shows histone acetyltransferase activity toward free histones.; FUNCTION: [Isoform B]: Does not show histone acetyltransferase activity toward free histones. KEYWORDS: Acyltransferase;Alternative splicing;Chromatin regulator;Chromosome partition;Reference proteome;Transferase " Q95U54,"PROTEIN NAMES: MMS19 nucleotide excision repair protein PROTEIN FAMILY: MET18/MMS19 family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the MET18/MMS19 family. FUNCTION: Key component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into apoproteins specifically involved in DNA metabolism and genomic integrity (By similarity). In the CIA complex, MMS19 acts as an adapter between early-acting CIA components and a subset of cellular target iron-sulfur proteins (By similarity). Essential for diploid cell cycles, organ growth and development. Regulates mitosis by binding to Xpd and thereby competing with the Xpd-mediated repression on the Cdk-activating kinase (CAK) complex. Regulates the centrosomal localization of the MT regulator tacc, a downstream target of aurA kinase. Binds to microtubules (MT). Regulates spindle and astral MT growth, MT stability and bundling. In neuroblasts, necessary for timely and coordinated spindle assembly and orientation which is necessary for mitotic progression. In young embryos, the maternal protein is important for progression through mitosis. KEYWORDS: Cell cycle;Cell division;Chromosome partition;Cytoplasm;Cytoskeleton;DNA damage;DNA repair;Mitosis;Nucleus;Reference proteome;Repeat SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, spindle Nucleus Cytoplasm Midbody Note=Localizes to the nucleus at the beginning of metaphase. " Q95US5,"PROTEIN NAMES: Beta-1,3-galactosyltransferase bre-5 (Bacillus thuringiensis toxin-resistant protein 5) (Bt toxin-resistant protein 5) PROTEIN FAMILY: Glycosyltransferase 31 family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the glycosyltransferase 31 family. FUNCTION: Transfers N-acetylgalactosamine onto mannose groups of carbohydrate substrates. Required for susceptibility to pore-forming crystal toxins in conjunction with bre-1, bre-2, bre-3, and bre-4. Involved in resistance to the nematotoxic C.cinerea galectin Cgl2. PATHWAY: Protein modification; protein glycosylation. KEYWORDS: Glycoprotein;Glycosyltransferase;Golgi apparatus;Insecticide resistance;Membrane;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus membrane ; Single-pass type II membrane protein " Q95YI5,"PROTEIN NAMES: UDP-sugar transporter UST74c (Protein fringe connection) PROTEIN FAMILY: TPT transporter family, SLC35D subfamily ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the TPT transporter family. SLC35D subfamily. FUNCTION: Involved in the import of UDP-sugars from the cytoplasm into the Golgi lumen. KEYWORDS: Golgi apparatus;Membrane;Phosphoprotein;Reference proteome;Sugar transport;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Golgi apparatus membrane; Multi-pass membrane protein. " Q95YL4,PROTEIN NAMES: Penta-EF hand domain-containing protein 2 (Apoptosis-linked gene 2 protein homolog B) (Dd-ALG-2b) (DdPEF-2) PROTEIN FAMILY: Peflin/Sorcin family ORGANISM: Dictyostelium discoideum (Social amoeba) SIMILARITY: Belongs to the Peflin/Sorcin family. KEYWORDS: Calcium;Cytoplasm;Membrane;Metal-binding;Reference proteome;Repeat SUBCELLULAR LOCATION: Cytoplasm Membrane ; Peripheral membrane protein. Note=Membrane-associated in the presence of Ca(2+). Q95ZQ4,"PROTEIN NAMES: 5'-AMP-activated protein kinase catalytic subunit alpha-2 (AMPK subunit alpha-2) PROTEIN FAMILY: Protein kinase superfamily, CAMK Ser/Thr protein kinase family, SNF1 subfamily ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily. FUNCTION: Acts as a sensor that couples lifespan to information about energy levels and insulin-like signals. Role in motility and response to oxidative stress. Involved in the establishment of germline stem cell (GSC) quiescence during dauer development. Plays a role in axon regrowth after axotomy in PLM neurons. Plays a role in the maintenance of glycogen stores which are necessary for resistance to hyperosmotic stress. Plays a role in the regulation of flp-7 secretion from ASI neurons. Keeps the CREB-regulated transcription coactivator 1 homolog crtc-1 inactive which in turn inhibits flp-7 secretion. Following serotonin signaling, derepresses crtc-1 which stimulates flp-7 secretion and subsequent body fat loss. KEYWORDS: Alternative splicing;ATP-binding;Kinase;Nucleotide-binding;Phosphoprotein;Reference proteome;Serine/threonine-protein kinase;Stress response;Transferase " Q95ZV7,"PROTEIN NAMES: Discoidin domain-containing receptor tyrosine kinase B (Discoidin domain-containing receptor B) PROTEIN FAMILY: Protein kinase superfamily, Tyr protein kinase family, Insulin receptor subfamily ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily. FUNCTION: Tyrosine-protein kinase receptor which, together with ddr-1, is involved in axon guidance to establish the tracts for the ventral and dorsal nerve cords during nervous system development. Acts upstream of the adapter shc-1, and the tyrosine kinase receptors svh-1 and svh-2 to regulate axon regeneration following injury in D-type motor neurons. May mediate axon regeneration in association with the collagen emb-9. KEYWORDS: Alternative splicing;ATP-binding;Cell membrane;Cell projection;Disulfide bond;Glycoprotein;Kinase;Membrane;Neurogenesis;Nucleotide-binding;Receptor;Reference proteome;Repeat;Signal;Transferase;Transmembrane;Transmembrane helix;Tyrosine-protein kinase SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein Cell projection, axon Perikaryon " Q960N3,PROTEIN NAMES: Protein cortex PROTEIN FAMILY: WD repeat CORT family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the WD repeat CORT family. FUNCTION: Female meiosis-specific activator of the anaphase promoting complex/cyclosome (APC/C). Required for the completion of meiosis in oocytes. Activates the ubiquitin ligase activity and substrate specificity of APC/C and triggers the sequential degradation of mitotic cyclins in meiosis. Promotes the ubiquitination and degradation of CycA early in meiosis I and the degradation of CycB and CycB3 after egg activation. Promotes degradation of mtrm at the oocyte-to-embryo transition. KEYWORDS: Cytoplasm;Developmental protein;Differentiation;Meiosis;Oogenesis;Reference proteome;Repeat;Ubl conjugation;WD repeat SUBCELLULAR LOCATION: Cytoplasm Q960X8,"PROTEIN NAMES: Hepatocyte growth factor-regulated tyrosine kinase substrate ORGANISM: Drosophila melanogaster (Fruit fly) FUNCTION: Essential role in endosome membrane invagination and formation of multivesicular bodies, MVBs. Required during gastrulation and appears to regulate early embryonic signaling pathways. Inhibits tyrosine kinase receptor signaling by promoting degradation of the tyrosine-phosphorylated, active receptor, potentially by sorting activated receptors into MVBs. The MVBs are then trafficked to the lysosome where their contents are degraded. KEYWORDS: 3D-structure;Alternative splicing;Cytoplasm;Developmental protein;Metal-binding;Phosphoprotein;Reference proteome;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cytoplasm, cell cortex Cytoplasm, perinuclear region Note=Locates to vesicles present in the perinuclear regions of muscle cells and in the periphery of Garland cells of third-instar larvae. " Q96290,"PROTEIN NAMES: Monosaccharide-sensing protein 1 (Monosaccharide transporter 1) (Sugar transporter MSSP1) (Sugar transporter MT1) (Tonoplast monosaccharide transporter 1) (AtTMT1) PROTEIN FAMILY: Major facilitator superfamily, Sugar transporter family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. FUNCTION: Sugar proton-coupled antiporter which contributes to vacuolar sugar import (e.g. monosaccharides including glucose, sucrose and fructose), particularly during stress responses (e.g. in response to cold). Required for cytosolic glucose homeostasis. KEYWORDS: Membrane;Phosphoprotein;Reference proteome;Sugar transport;Transmembrane;Transmembrane helix;Transport;Vacuole SUBCELLULAR LOCATION: Vacuole membrane ; Multi-pass membrane protein " Q96318,PROTEIN NAMES: 12S seed storage protein CRC (Cruciferin 3) (AtCRU3) (Cruciferin C) (Legumin-type globulin Cruciferin1) (Legumin-type globulin storage protein CRU1) [Cleaved into: 12S seed storage protein CRC alpha chain (12S seed storage protein CRC acidic chain); 12S seed storage protein CRC beta chain (12S seed storage protein CRC basic chain)] PROTEIN FAMILY: 11S seed storage protein (globulins) family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the 11S seed storage protein (globulins) family. FUNCTION: Seed storage protein. KEYWORDS: Alternative splicing;Direct protein sequencing;Disulfide bond;Phosphoprotein;Reference proteome;Seed storage protein;Signal;Storage protein;Vacuole SUBCELLULAR LOCATION: Protein storage vacuole Q96327,"PROTEIN NAMES: ERBB-3 BINDING PROTEIN 1 (AtEBP1) (Proliferation-associated protein G2p) (AtG2) (Protein CELL-PROLIFERATION-RELATED) (AtCPR) PROTEIN FAMILY: Peptidase M24 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the peptidase M24 family. FUNCTION: Binds RNA. Associates with 28S, 18S and 5.8S mature rRNAs, several rRNA precursors and probably U3 small nucleolar RNA. May be involved in regulation of intermediate and late steps of rRNA processing. May be involved in ribosome assembly (By similarity). Required for expression of cell cycle genes such as CYCD3-1, RNR2A and CDKB1-1. Promotes, in a dose- and auxin-dependent manner, organ growth by stimulating both cell proliferation and expansion, via the regulation of RBR1 levels. KEYWORDS: Alternative splicing;Auxin signaling pathway;Developmental protein;Nucleus;Reference proteome;Ribonucleoprotein;RNA-binding;rRNA processing SUBCELLULAR LOCATION: Nucleus " Q96329,"PROTEIN NAMES: Acyl-coenzyme A oxidase 4, peroxisomal (AOX 4) (G6p) (Short-chain acyl-CoA oxidase) (AtCX4) (AtG6) (SAOX) PROTEIN FAMILY: Acyl-CoA dehydrogenase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the acyl-CoA dehydrogenase family. FUNCTION: Catalyzes the desaturation of short-chain acyl-CoAs to 2-trans-enoyl-CoAs. Active on butyryl-CoA (C4), hexanoyl-CoA (C6), and octanoyl-CoA (C8). Has no activity as acyl-CoA dehydrogenase or on crotonyl-CoA (an unsaturated C4:1 carbocyclic ester) or glutaryl-CoA (a dicarboxylic ester). Peroxisomal fatty acid beta-oxidation is essential for embryo development. Acts as a suppressor of transcriptional silencing. Required for nuclear histone acetylation and DNA demethylation at some endogenous genomic loci. KEYWORDS: 3D-structure;Acetylation;FAD;Fatty acid metabolism;Flavoprotein;Glyoxysome;Lipid metabolism;Oxidoreductase;Peroxisome;Reference proteome SUBCELLULAR LOCATION: Glyoxysome Peroxisome " Q96565,"PROTEIN NAMES: 3-aminomethylindole N-methyltransferase PROTEIN FAMILY: Class I-like SAM-binding methyltransferase superfamily, Cation-independent O-methyltransferase family ORGANISM: Hordeum vulgare subsp. vulgare (Domesticated barley) SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O-methyltransferase family. FUNCTION: Methylates 3-aminomethylindole (AMI) and N-methyl-3-aminomethylindole (MAMI), two substrates involved in gramine biosynthesis, a toxic indole alkaloid. Can use S-adenosyl-L-methionine (AdoMet) as a methyl donor. Unable to mediate caffeic acid O-methylation. PATHWAY: Alkaloid biosynthesis. KEYWORDS: Methyltransferase;Reference proteome;S-adenosyl-L-methionine;Transferase MISCELLANEOUS: Present and expressed only in cultivars containing gramine (e.g. cv. 5172-28:4, cv. 5172-39:9, cv. 5175-50:20, cv. Hordeum vulgare ssp. spontaneum accession 5, cv. Golf, cv. Lina, cv. Barke, cv. Sultan5, cv. Igri, cv. Salome and cv. Osiris) but not detected in cv. CI 16145, cv. CI 11506, cv. Mona, cv. Morex and cv. Maythorpe." Q968Z6,PROTEIN NAMES: Protein psiA (Prespore-cell-inducing factor A) PROTEIN FAMILY: Prespore-cell-inducing factor family ORGANISM: Dictyostelium discoideum (Social amoeba) SIMILARITY: Belongs to the prespore-cell-inducing factor family. FUNCTION: Growth factor that can induce isolated amoebae to differentiate into prespore cells. KEYWORDS: Developmental protein;Differentiation;Direct protein sequencing;Glycoprotein;Growth factor;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted Q969D9,"PROTEIN NAMES: Thymic stromal lymphopoietin ORGANISM: Homo sapiens (Human) FUNCTION: [Isoform 1]: Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c(+) dendritic cells. Can induce allergic inflammation by directly activating mast cells.; FUNCTION: [Isoform 2]: May act as an antimicrobial peptide in the oral cavity and on the skin. KEYWORDS: 3D-structure;Alternative splicing;Cytokine;Disulfide bond;Glycoprotein;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted " Q969J5,"PROTEIN NAMES: Interleukin-22 receptor subunit alpha-2 (IL-22 receptor subunit alpha-2) (IL-22R-alpha-2) (IL-22RA2) (Cytokine receptor class-II member 10) (Cytokine receptor family 2 member 10) (CRF2-10) (Cytokine receptor family type 2, soluble 1) (CRF2-S1) (Interleukin-22-binding protein) (IL-22BP) (IL22BP) (ZcytoR16) PROTEIN FAMILY: Type II cytokine receptor family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the type II cytokine receptor family. FUNCTION: Isoform 2 is a receptor for IL22. Binds to IL22, prevents interaction with the functional IL-22R complex and blocks the activity of IL22 (in vitro). May play an important role as an IL22 antagonist in the regulation of inflammatory responses.; FUNCTION: Isoform 1 may play a role in establishing and maintaining successful pregnancy. KEYWORDS: 3D-structure;Alternative splicing;Direct protein sequencing;Disulfide bond;Glycoprotein;Receptor;Reference proteome;Repeat;Secreted;Signal SUBCELLULAR LOCATION: Secreted " Q969P0,"PROTEIN NAMES: Immunoglobulin superfamily member 8 (IgSF8) (CD81 partner 3) (Glu-Trp-Ile EWI motif-containing protein 2) (EWI-2) (Keratinocytes-associated transmembrane protein 4) (KCT-4) (LIR-D1) (Prostaglandin regulatory-like protein) (PGRL) (CD antigen CD316) ORGANISM: Homo sapiens (Human) FUNCTION: May play a key role in diverse functions ascribed to CD81 and CD9 such as oocytes fertilization or hepatitis C virus function. May regulate proliferation and differentiation of keratinocytes. May be a negative regulator of cell motility: suppresses T-cell mobility coordinately with CD81, associates with CD82 to suppress prostate cancer cell migration, regulates epidermoid cell reaggregation and motility on laminin-5 with CD9 and CD81 as key linkers. May also play a role on integrin-dependent morphology and motility functions. May participate in the regulation of neurite outgrowth and maintenance of the neural network in the adult brain. KEYWORDS: Alternative splicing;Cell membrane;Direct protein sequencing;Disulfide bond;Glycoprotein;Immunoglobulin domain;Lipoprotein;Membrane;Palmitate;Phosphoprotein;Reference proteome;Repeat;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass membrane protein. " Q969S8,"PROTEIN NAMES: Polyamine deacetylase HDAC10 (Histone deacetylase 10) (HD10) PROTEIN FAMILY: Histone deacetylase family, HD type 2 subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the histone deacetylase family. HD type 2 subfamily. FUNCTION: Polyamine deacetylase (PDAC), which acts preferentially on N(8)-acetylspermidine, and also on acetylcadaverine and acetylputrescine. Exhibits attenuated catalytic activity toward N(1),N(8)-diacetylspermidine and very low activity, if any, toward N(1)-acetylspermidine. Histone deacetylase activity has been observed in vitro. Has also been shown to be involved in MSH2 deacetylation. The physiological relevance of protein/histone deacetylase activity is unclear and could be very weak. May play a role in the promotion of late stages of autophagy, possibly autophagosome-lysosome fusion and/or lysosomal exocytosis in neuroblastoma cells. May play a role in homologous recombination. May promote DNA mismatch repair. KEYWORDS: Alternative splicing;Autophagy;Cytoplasm;DNA damage;DNA recombination;DNA repair;Hydrolase;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Zinc SUBCELLULAR LOCATION: Cytoplasm Nucleus Note=Excluded from nucleoli. MISCELLANEOUS: Like some other members of the HD type 2 subfamily, such as HDAC4, inhibited by the antitumor drug trichostatin A (TSA).; MISCELLANEOUS: [Isoform 4]: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay." Q969X2,"PROTEIN NAMES: Alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase 6 (GalNAc alpha-2,6-sialyltransferase VI) (ST6GalNAc VI) (ST6GalNAcVI) (hST6GalNAc VI) (Sialyltransferase 7F) (SIAT7-F) PROTEIN FAMILY: Glycosyltransferase 29 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the glycosyltransferase 29 family. FUNCTION: Transfers the sialyl group (N-acetyl-alpha-neuraminyl or NeuAc) from CMP-NeuAc onto glycoproteins and glycolipids, forming an alpha-2,6-linkage. Produces branched type disialyl structures by transfer of a sialyl group onto the GalNAc or GlcNAc residue inside backbone core chains having a terminal sialic acid with an alpha-2,3-linkage on Gal. ST6GalNAcVI prefers glycolipids to glycoproteins, predominantly catalyzing the biosynthesis of ganglioside GD1alpha from GM1b. Besides GMb1, MSGG and other glycolipids, it shows activity towards sialyl Lc4Cer generating disialyl Lc4Cer, which can lead to the synthesis of disialyl Lewis a (Le(a)), suggested to be a cancer-associated antigen. Also has activity toward GD1a and GT1b, and can generate DSGG (disialylgalactosylgloboside) from MSGG (monosialylgalactosylgloboside) (By similarity). KEYWORDS: Alternative splicing;Disulfide bond;Glycoprotein;Glycosyltransferase;Golgi apparatus;Lipid metabolism;Membrane;Reference proteome;Sialic acid;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus membrane ; Single-pass type II membrane protein MISCELLANEOUS: The carbohydrate antigen disialyl Lewis a, which is at least partly synthesized by ST6GALNAC6, is a normal counterpart of sialyl Lewis a, better known as CA19-9, an antigen widely used as a serum marker for diagnosis of cancers in the digestive track. Disialyl Lewis a is predominantly expressed in non-malignant epithelial cells of the digestive organs, while sialyl Lewis a is preferentially expressed in cancers. Disialyl Lewis a in normal epithelial cells serves as a ligand for immunosuppressive receptors, such as SIGLEC7 and SIGLEC9, expressed on resident monocytes/macrophages and maintains immunological homeostasis of mucosal membranes in digestive organs. Sialyl Lewis a, as well as its positional isomer sialyl Lewis x, serves as a ligand for vascular cell adhesion molecule E-selectin and facilitates hematogenous metastasis through mediating adhesion of circulating cancer cells to vascular endothelium." Q96BD8,"PROTEIN NAMES: Spindle and kinetochore-associated protein 1 PROTEIN FAMILY: SKA1 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the SKA1 family. FUNCTION: Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. Required for timely anaphase onset during mitosis, when chromosomes undergo bipolar attachment on spindle microtubules leading to silencing of the spindle checkpoint. The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. Affinity for microtubules is synergistically enhanced in the presence of the ndc-80 complex and may allow the ndc-80 complex to track depolymerizing microtubules. In the complex, it mediates the interaction with microtubules. KEYWORDS: 3D-structure;Acetylation;Alternative splicing;Cell cycle;Cell division;Centromere;Chromosome;Coiled coil;Cytoplasm;Cytoskeleton;Kinetochore;Microtubule;Mitosis;Reference proteome SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, spindle Chromosome, centromere, kinetochore Note=Localizes to the outer kinetochore and spindle microtubules during mitosis in a NDC80 complex-dependent manner. Localizes to both the mitotic spindle and kinetochore-associated proteins. Associates with kinetochores following microtubule attachment from prometaphase, through mid-anaphase and then vanishes in telophase. " Q96BI1,"PROTEIN NAMES: Solute carrier family 22 member 18 (Beckwith-Wiedemann syndrome chromosomal region 1 candidate gene A protein) (Efflux transporter-like protein) (Imprinted multi-membrane-spanning polyspecific transporter-related protein 1) (Organic cation transporter-like protein 2) (ORCTL-2) (Solute carrier family 22 member 1-like) (Tumor-suppressing STF cDNA 5 protein) (Tumor-suppressing subchromosomal transferable fragment candidate gene 5 protein) (p45-Beckwith-Wiedemann region 1 A) (p45-BWR1A) PROTEIN FAMILY: Major facilitator superfamily, Organic cation transporter family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the major facilitator (TC 2.A.1) superfamily. Organic cation transporter (TC 2.A.1.19) family. FUNCTION: May act as a transporter of organic cations based on a proton efflux antiport mechanism. May play a role in the transport of chloroquine and quinidine-related compounds in kidney. KEYWORDS: Cell membrane;Disease variant;Ion transport;Membrane;Reference proteome;Symport;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Apical cell membrane ; Multi-pass membrane protein Note=Localized at the apical membrane surface of renal proximal tubules. " Q96BR6,PROTEIN NAMES: Zinc finger protein 669 PROTEIN FAMILY: Krueppel C2H2-type zinc-finger protein family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein family. FUNCTION: May be involved in transcriptional regulation. KEYWORDS: Alternative splicing;DNA-binding;Metal-binding;Nucleus;Reference proteome;Repeat;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Q96BZ4,"PROTEIN NAMES: 5'-3' exonuclease PLD4 (Choline phosphatase 4) (Phosphatidylcholine-hydrolyzing phospholipase D4) (Phospholipase D family member 4) (Phospholipase D4) (PLD 4) PROTEIN FAMILY: Phospholipase D family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the phospholipase D family. FUNCTION: 5'->3' DNA exonuclease which digests single-stranded DNA (ssDNA). Regulates inflammatory cytokine responses via the degradation of nucleic acids, by reducing the concentration of ssDNA able to stimulate TLR9, a nucleotide-sensing receptor. Involved in phagocytosis of activated microglia. KEYWORDS: Cytoplasmic vesicle;Endoplasmic reticulum;Endosome;Exonuclease;Glycoprotein;Golgi apparatus;Hydrolase;Immunity;Inflammatory response;Innate immunity;Membrane;Nuclease;Nucleus;Reference proteome;Repeat;Signal-anchor;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Single-pass type II membrane protein Golgi apparatus, trans-Golgi network membrane ; Single-pass type II membrane protein Nucleus Early endosome Cytoplasmic vesicle, phagosome Note=Activation of microglia induces translocation of PLD4 from the nucleus to the phagosomes. " Q96C10,"PROTEIN NAMES: ATP-dependent RNA helicase DHX58 (ATP-dependent helicase LGP2) (Protein D11Lgp2 homolog) (RIG-I-like receptor 3) (RLR-3) (RIG-I-like receptor LGP2) (RLR) PROTEIN FAMILY: Helicase family, RLR subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the helicase family. RLR subfamily. FUNCTION: Acts as a regulator of RIGI and IFIH1/MDA5 mediated antiviral signaling. Cannot initiate antiviral signaling as it lacks the CARD domain required for activating MAVS/IPS1-dependent signaling events. Can have both negative and positive regulatory functions related to RIGI and IFIH1/MDA5 signaling and this role in regulating signaling may be complex and could probably depend on characteristics of the infecting virus or target cells, or both. Its inhibitory action on RIG-I signaling may involve the following mechanisms: competition with RIGI for binding to the viral RNA, binding to RIGI and inhibiting its dimerization and interaction with MAVS/IPS1, competing with IKBKE in its binding to MAVS/IPS1 thereby inhibiting activation of interferon regulatory factor 3 (IRF3). Its positive regulatory role may involve unwinding or stripping nucleoproteins of viral RNA thereby facilitating their recognition by RIGI and IFIH1/MDA5. Involved in the innate immune response to various RNA viruses and some DNA viruses such as poxviruses and coronavirus SARS-CoV-2, and also to the bacterial pathogen Listeria monocytogenes. Can bind both ssRNA and dsRNA, with a higher affinity for dsRNA. Shows a preference to 5'-triphosphorylated RNA, although it can recognize RNA lacking a 5'-triphosphate. KEYWORDS: 3D-structure;Antiviral defense;ATP-binding;Coiled coil;Cytoplasm;Helicase;Host-virus interaction;Hydrolase;Immunity;Innate immunity;Metal-binding;Nucleotide-binding;Reference proteome;RNA-binding;Zinc SUBCELLULAR LOCATION: Cytoplasm " Q96C12,"PROTEIN NAMES: Armadillo repeat-containing protein 5 ORGANISM: Homo sapiens (Human) FUNCTION: Involved in fetal development, T-cell function and adrenal gland growth homeostasis (By similarity). Negatively regulates adrenal cells survival. Plays a role in steroidogenesis, modulates steroidogenic enzymes expression and cortisol production. KEYWORDS: Alternative splicing;Cushing syndrome;Cytoplasm;Disease variant;Phosphoprotein;Reference proteome;Repeat SUBCELLULAR LOCATION: Cytoplasm " Q96DT7,PROTEIN NAMES: Zinc finger and BTB domain-containing protein 10 (Zinc finger protein RIN ZF) ORGANISM: Homo sapiens (Human) FUNCTION: May be involved in transcriptional regulation. KEYWORDS: 3D-structure;Alternative splicing;DNA-binding;Isopeptide bond;Metal-binding;Methylation;Nucleus;Phosphoprotein;Reference proteome;Repeat;Transcription;Transcription regulation;Ubl conjugation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Q96E40,"PROTEIN NAMES: Sperm acrosome-associated protein 9 ORGANISM: Homo sapiens (Human) FUNCTION: Microtubule inner protein (MIP) part of the dynein-decorated doublet microtubules (DMTs) of multiciliated respiratory cells and the distal singlet microtubules of monoflagellated spermatozoa. Forms both spirals and striations within ciliary microtubules. May stabilize the protofilaments to which they are bound. KEYWORDS: 3D-structure;Alternative splicing;Cell projection;Cilium;Cytoplasm;Cytoplasmic vesicle;Cytoskeleton;Flagellum;Nucleus;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Cytoplasmic vesicle, secretory vesicle, acrosome Cytoplasm, cytoskeleton, cilium basal body Cell projection, cilium, flagellum Nucleus Cytoplasm, cytoskeleton, cilium axoneme Note=In caudal sperms localizes onto sperm head. Is also present on midpiece and principle piece of sperm tails. Acrosome and sperm tail localization is regulated by Y-chromosome. " Q96E52,"PROTEIN NAMES: Metalloendopeptidase OMA1, mitochondrial (Metalloprotease-related protein 1) (MPRP-1) (Overlapping with the m-AAA protease 1 homolog) PROTEIN FAMILY: Peptidase M48 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the peptidase M48 family. FUNCTION: Metalloprotease that is part of the quality control system in the inner membrane of mitochondria. Activated in response to various mitochondrial stress, leading to the proteolytic cleavage of target proteins, such as OPA1, UQCC3 and DELE1. Involved in the fusion of the mitochondrial inner membranes by mediating cleavage of OPA1 at S1 position, generating the soluble OPA1 (S-OPA1), which cooperates with the membrane form (L-OPA1) to coordinate the fusion of mitochondrial inner membranes. Following stress conditions that induce loss of mitochondrial membrane potential, mediates cleavage of OPA1, leading to excess production of soluble OPA1 (S-OPA1) and negative regulation of mitochondrial fusion. Involved in mitochondrial safeguard in response to transient mitochondrial membrane depolarization (flickering) by catalyzing cleavage of OPA1, leading to excess production of S-OPA1, preventing mitochondrial hyperfusion (By similarity). Also acts as a regulator of apoptosis: upon BAK and BAX aggregation, mediates cleavage of OPA1, leading to the remodeling of mitochondrial cristae and allowing the release of cytochrome c from mitochondrial cristae. In depolarized mitochondria, may also act as a backup protease for PINK1 by mediating PINK1 cleavage and promoting its subsequent degradation by the proteasome. May also cleave UQCC3 in response to mitochondrial depolarization. Also acts as an activator of the integrated stress response (ISR): in response to mitochondrial stress, mediates cleavage of DELE1 to generate the processed form of DELE1 (S-DELE1), which translocates to the cytosol and activates EIF2AK1/HRI to trigger the ISR. Its role in mitochondrial quality control is essential for regulating lipid metabolism as well as to maintain body temperature and energy expenditure under cold-stress conditions (By similarity). Binds cardiolipin, possibly regulating its protein turnover (By similarity). Required for the stability of the respiratory supercomplexes (By similarity). KEYWORDS: Alternative splicing;Autocatalytic cleavage;Disulfide bond;Hydrolase;Lipid-binding;Membrane;Metal-binding;Metalloprotease;Mitochondrion;Mitochondrion inner membrane;Protease;Reference proteome;Transit peptide;Transmembrane;Transmembrane helix;Zinc;Zymogen SUBCELLULAR LOCATION: Mitochondrion inner membrane ; Single-pass membrane protein " Q96EB1,"PROTEIN NAMES: Elongator complex protein 4 (hELP4) (PAX6 neighbor gene protein) PROTEIN FAMILY: ELP4 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the ELP4 family. FUNCTION: Component of the elongator complex which is required for multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl uridine), mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U (5-carbamoylmethyl uridine). The elongator complex catalyzes the formation of carboxymethyluridine in the wobble base at position 34 in tRNAs. PATHWAY: tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis. KEYWORDS: Alternative splicing;Cytoplasm;Nucleus;Reference proteome;tRNA processing SUBCELLULAR LOCATION: Cytoplasm Nucleus " Q96EB6,"PROTEIN NAMES: NAD-dependent protein deacetylase sirtuin-1 (hSIRT1) (NAD-dependent protein deacylase sirtuin-1) (Regulatory protein SIR2 homolog 1) (SIR2-like protein 1) (hSIR2) [Cleaved into: SirtT1 75 kDa fragment (75SirT1)] PROTEIN FAMILY: Sirtuin family, Class I subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the sirtuin family. Class I subfamily. FUNCTION: NAD-dependent protein deacetylase that links transcriptional regulation directly to intracellular energetics and participates in the coordination of several separated cellular functions such as cell cycle, response to DNA damage, metabolism, apoptosis and autophagy. Can modulate chromatin function through deacetylation of histones and can promote alterations in the methylation of histones and DNA, leading to transcriptional repression. Deacetylates a broad range of transcription factors and coregulators, thereby regulating target gene expression positively and negatively. Serves as a sensor of the cytosolic ratio of NAD(+)/NADH which is altered by glucose deprivation and metabolic changes associated with caloric restriction. Is essential in skeletal muscle cell differentiation and in response to low nutrients mediates the inhibitory effect on skeletal myoblast differentiation which also involves 5'-AMP-activated protein kinase (AMPK) and nicotinamide phosphoribosyltransferase (NAMPT) (By similarity). Component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD(+)/NADP(+) ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. Deacetylates 'Lys-266' of SUV39H1, leading to its activation. Inhibits skeletal muscle differentiation by deacetylating PCAF and MYOD1. Deacetylates H2A and 'Lys-26' of H1-4. Deacetylates 'Lys-16' of histone H4 (in vitro). Involved in NR0B2/SHP corepression function through chromatin remodeling: Recruited to LRH1 target gene promoters by NR0B2/SHP thereby stimulating histone H3 and H4 deacetylation leading to transcriptional repression. Proposed to contribute to genomic integrity via positive regulation of telomere length; however, reports on localization to pericentromeric heterochromatin are conflicting (By similarity). Proposed to play a role in constitutive heterochromatin (CH) formation and/or maintenance through regulation of the available pool of nuclear SUV39H1. Upon oxidative/metabolic stress decreases SUV39H1 degradation by inhibiting SUV39H1 polyubiquitination by MDM2. This increase in SUV39H1 levels enhances SUV39H1 turnover in CH, which in turn seems to accelerate renewal of the heterochromatin which correlates with greater genomic integrity during stress response. Deacetylates 'Lys-382' of p53/TP53 and impairs its ability to induce transcription-dependent proapoptotic program and modulate cell senescence. Deacetylates TAF1B and thereby represses rDNA transcription by the RNA polymerase I (By similarity). Deacetylates MYC, promotes the association of MYC with MAX and decreases MYC stability leading to compromised transformational capability. Deacetylates FOXO3 in response to oxidative stress thereby increasing its ability to induce cell cycle arrest and resistance to oxidative stress but inhibiting FOXO3-mediated induction of apoptosis transcriptional activity; also leading to FOXO3 ubiquitination and protesomal degradation. Appears to have a similar effect on MLLT7/FOXO4 in regulation of transcriptional activity and apoptosis. Deacetylates DNMT1; thereby impairs DNMT1 methyltransferase-independent transcription repressor activity, modulates DNMT1 cell cycle regulatory function and DNMT1-mediated gene silencing. Deacetylates RELA/NF-kappa-B p65 thereby inhibiting its transactivating potential and augments apoptosis in response to TNF-alpha. Deacetylates HIF1A, KAT5/TIP60, RB1 and HIC1. Deacetylates FOXO1 resulting in its nuclear retention and enhancement of its transcriptional activity leading to increased gluconeogenesis in liver. Inhibits E2F1 transcriptional activity and apoptotic function, possibly by deacetylation. Involved in HES1- and HEY2-mediated transcriptional repression. In cooperation with MYCN seems to be involved in transcriptional repression of DUSP6/MAPK3 leading to MYCN stabilization by phosphorylation at 'Ser-62'. Deacetylates MEF2D. Required for antagonist-mediated transcription suppression of AR-dependent genes which may be linked to local deacetylation of histone H3. Represses HNF1A-mediated transcription (By similarity). Required for the repression of ESRRG by CREBZF. Deacetylates NR1H3 and NR1H2 and deacetylation of NR1H3 at 'Lys-434' positively regulates transcription of NR1H3:RXR target genes, promotes NR1H3 proteasomal degradation and results in cholesterol efflux; a promoter clearing mechanism after reach round of transcription is proposed. Involved in lipid metabolism: deacetylates LPIN1, thereby inhibiting diacylglycerol synthesis. Implicated in regulation of adipogenesis and fat mobilization in white adipocytes by repression of PPARG which probably involves association with NCOR1 and SMRT/NCOR2 (By similarity). Deacetylates p300/EP300 and PRMT1 (By similarity). Deacetylates ACSS2 leading to its activation, and HMGCS1 deacetylation. Involved in liver and muscle metabolism. Through deacetylation and activation of PPARGC1A is required to activate fatty acid oxidation in skeletal muscle under low-glucose conditions and is involved in glucose homeostasis. Involved in regulation of PPARA and fatty acid beta-oxidation in liver. Involved in positive regulation of insulin secretion in pancreatic beta cells in response to glucose; the function seems to imply transcriptional repression of UCP2. Proposed to deacetylate IRS2 thereby facilitating its insulin-induced tyrosine phosphorylation. Deacetylates SREBF1 isoform SREBP-1C thereby decreasing its stability and transactivation in lipogenic gene expression. Involved in DNA damage response by repressing genes which are involved in DNA repair, such as XPC and TP73, deacetylating XRCC6/Ku70, and facilitating recruitment of additional factors to sites of damaged DNA, such as SIRT1-deacetylated NBN can recruit ATM to initiate DNA repair and SIRT1-deacetylated XPA interacts with RPA2. Also involved in DNA repair of DNA double-strand breaks by homologous recombination and specifically single-strand annealing independently of XRCC6/Ku70 and NBN. Promotes DNA double-strand breaks by mediating deacetylation of SIRT6. Transcriptional suppression of XPC probably involves an E2F4:RBL2 suppressor complex and protein kinase B (AKT) signaling. Transcriptional suppression of TP73 probably involves E2F4 and PCAF. Deacetylates WRN thereby regulating its helicase and exonuclease activities and regulates WRN nuclear translocation in response to DNA damage. Deacetylates APEX1 at 'Lys-6' and 'Lys-7' and stimulates cellular AP endonuclease activity by promoting the association of APEX1 to XRCC1. Catalyzes deacetylation of ERCC4/XPF, thereby impairing interaction with ERCC1 and nucleotide excision repair (NER). Increases p53/TP53-mediated transcription-independent apoptosis by blocking nuclear translocation of cytoplasmic p53/TP53 and probably redirecting it to mitochondria. Deacetylates XRCC6/Ku70 at 'Lys-539' and 'Lys-542' causing it to sequester BAX away from mitochondria thereby inhibiting stress-induced apoptosis. Is involved in autophagy, presumably by deacetylating ATG5, ATG7 and MAP1LC3B/ATG8. Deacetylates AKT1 which leads to enhanced binding of AKT1 and PDK1 to PIP3 and promotes their activation. Proposed to play role in regulation of STK11/LBK1-dependent AMPK signaling pathways implicated in cellular senescence which seems to involve the regulation of the acetylation status of STK11/LBK1. Can deacetylate STK11/LBK1 and thereby increase its activity, cytoplasmic localization and association with STRAD; however, the relevance of such activity in normal cells is unclear. In endothelial cells is shown to inhibit STK11/LBK1 activity and to promote its degradation. Deacetylates SMAD7 at 'Lys-64' and 'Lys-70' thereby promoting its degradation. Deacetylates CIITA and augments its MHC class II transactivation and contributes to its stability. Deacetylates MECOM/EVI1. Deacetylates PML at 'Lys-487' and this deacetylation promotes PML control of PER2 nuclear localization. During the neurogenic transition, represses selective NOTCH1-target genes through histone deacetylation in a BCL6-dependent manner and leading to neuronal differentiation. Regulates the circadian expression of several core clock genes, including BMAL1, RORC, PER2 and CRY1 and plays a critical role in maintaining a controlled rhythmicity in histone acetylation, thereby contributing to circadian chromatin remodeling. Deacetylates BMAL1 and histones at the circadian gene promoters in order to facilitate repression by inhibitory components of the circadian oscillator (By similarity). Deacetylates PER2, facilitating its ubiquitination and degradation by the proteasome (By similarity). Protects cardiomyocytes against palmitate-induced apoptosis (By similarity). Deacetylates XBP1 isoform 2; deacetylation decreases protein stability of XBP1 isoform 2 and inhibits its transcriptional activity. Deacetylates PCK1 and directs its activity toward phosphoenolpyruvate production promoting gluconeogenesis. Involved in the CCAR2-mediated regulation of PCK1 and NR1D1. Deacetylates CTNB1 at 'Lys-49'. In POMC (pro-opiomelanocortin) neurons, required for leptin-induced activation of PI3K signaling (By similarity). In addition to protein deacetylase activity, also acts as a protein-lysine deacylase by mediating protein depropionylation and decrotonylation. Mediates depropionylation of Osterix (SP7) (By similarity). Catalyzes decrotonylation of histones; it however does not represent a major histone decrotonylase. Deacetylates SOX9; promoting SOX9 nuclear localization and transactivation activity (By similarity). Involved in the regulation of centrosome duplication. Deacetylates CENATAC in G1 phase, allowing for SASS6 accumulation on the centrosome and subsequent procentriole assembly. Deacetylates NDC80/HEC1.; FUNCTION: [Isoform 2]: Deacetylates 'Lys-382' of p53/TP53, however with lower activity than isoform 1. In combination, the two isoforms exert an additive effect. Isoform 2 regulates p53/TP53 expression and cellular stress response and is in turn repressed by p53/TP53 presenting a SIRT1 isoform-dependent auto-regulatory loop.; FUNCTION: [SirtT1 75 kDa fragment]: Catalytically inactive 75SirT1 may be involved in regulation of apoptosis. May be involved in protecting chondrocytes from apoptotic death by associating with cytochrome C and interfering with apoptosome assembly.; FUNCTION: (Microbial infection) In case of HIV-1 infection, interacts with and deacetylates the viral Tat protein. The viral Tat protein inhibits SIRT1 deacetylation activity toward RELA/NF-kappa-B p65, thereby potentiates its transcriptional activity and SIRT1 is proposed to contribute to T-cell hyperactivation during infection. KEYWORDS: 3D-structure;Acetylation;Alternative splicing;Apoptosis;Biological rhythms;Cytoplasm;Developmental protein;Differentiation;Host-virus interaction;Metal-binding;Methylation;Mitochondrion;Myogenesis;NAD;Nucleus;Phosphoprotein;Reference proteome;S-nitrosylation;Transcription;Transcription regulation;Transferase;Ubl conjugation;Zinc SUBCELLULAR LOCATION: Nucleus, PML body Cytoplasm Nucleus Note=Recruited to the nuclear bodies via its interaction with PML. Colocalized with APEX1 in the nucleus. May be found in nucleolus, nuclear euchromatin, heterochromatin and inner membrane. Shuttles between nucleus and cytoplasm (By similarity). Colocalizes in the nucleus with XBP1 isoform 2.; SUBCELLULAR LOCATION: [SirtT1 75 kDa fragment]: Cytoplasm Mitochondrion MISCELLANEOUS: Red wine, which contains resveratrol, may participate in activation of sirtuin proteins, and may therefore participate in an extended lifespan as it has been observed in yeast.; MISCELLANEOUS: Calf histone H1 is used as substrate in the in vitro deacetylation assay. As, in vivo, interaction occurs between SIRT1 with H1-4, deacetylation has been validated only for H1-4.; MISCELLANEOUS: The reported ADP-ribosyltransferase activity of sirtuins is likely some inefficient side reaction of the deacetylase activity and may not be physiologically relevant." Q96EG3,PROTEIN NAMES: Zinc finger protein 837 PROTEIN FAMILY: Krueppel C2H2-type zinc-finger protein family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein family. FUNCTION: May be involved in transcriptional regulation. KEYWORDS: DNA-binding;Metal-binding;Nucleus;Reference proteome;Repeat;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Q96EP5,"PROTEIN NAMES: DAZ-associated protein 1 (Deleted in azoospermia-associated protein 1) ORGANISM: Homo sapiens (Human) FUNCTION: RNA-binding protein, which may be required during spermatogenesis. KEYWORDS: 3D-structure;Acetylation;Alternative splicing;Cytoplasm;Developmental protein;Differentiation;Direct protein sequencing;Methylation;Nucleus;Reference proteome;Repeat;RNA-binding;Spermatogenesis SUBCELLULAR LOCATION: Cytoplasm Nucleus Note=Predominantly cytoplasmic (By similarity). Nuclear at some stages of spermatozoides development. In midpachytene spermatocytes, it is localized in both the cytoplasm and the nuclei and is clearly excluded from the sex vesicles. In round spermatids, it localizes mainly in the nuclei, whereas in elongated spermatids, it localizes to the cytoplasm (By similarity). " Q96ER9,"PROTEIN NAMES: Mitochondrial potassium channel (MITOK) (Coiled-coil domain-containing protein 51) ORGANISM: Homo sapiens (Human) FUNCTION: Pore-forming subunit of the mitochondrial ATP-gated potassium channel (mitoK(ATP)). Together with ATP-binding subunit ABCB8/MITOSUR of the mitoK(ATP) channel, mediates ATP-dependent K(+) currents across the mitochondrial inner membrane. An increase in ATP intracellular levels closes the channel, inhibiting K(+) transport, whereas a decrease in ATP levels enhances K(+) uptake in the mitochondrial matrix. May contribute to the homeostatic control of cellular metabolism under stress conditions by regulating the mitochondrial matrix volume. KEYWORDS: Alternative splicing;Coiled coil;Ion channel;Ion transport;Membrane;Mitochondrion;Mitochondrion inner membrane;Phosphoprotein;Potassium;Potassium channel;Potassium transport;Reference proteome;Transit peptide;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Mitochondrion inner membrane ; Multi-pass membrane protein.; SUBCELLULAR LOCATION: [Isoform 1]: Mitochondrion inner membrane ; Multi-pass membrane protein " Q96EW2,PROTEIN NAMES: HSPB1-associated protein 1 (27 kDa heat shock protein-associated protein 1) (Protein associated with small stress protein 1) ORGANISM: Homo sapiens (Human) FUNCTION: May play a role in cellular stress response. KEYWORDS: Alternative splicing;Chromosomal rearrangement;Cytoplasm;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Q96EY1,"PROTEIN NAMES: DnaJ homolog subfamily A member 3, mitochondrial (DnaJ protein Tid-1) (hTid-1) (Hepatocellular carcinoma-associated antigen 57) (Tumorous imaginal discs protein Tid56 homolog) ORGANISM: Homo sapiens (Human) FUNCTION: Modulates apoptotic signal transduction or effector structures within the mitochondrial matrix. Affect cytochrome C release from the mitochondria and caspase 3 activation, but not caspase 8 activation. Isoform 1 increases apoptosis triggered by both TNF and the DNA-damaging agent mytomycin C; in sharp contrast, isoform 2 suppresses apoptosis. Can modulate IFN-gamma-mediated transcriptional activity. Isoform 2 may play a role in neuromuscular junction development as an effector of the MUSK signaling pathway. KEYWORDS: 3D-structure;Acetylation;Alternative splicing;Apoptosis;Cell membrane;Chaperone;Cytoplasm;Membrane;Metal-binding;Methylation;Mitochondrion;Phosphoprotein;Postsynaptic cell membrane;Reference proteome;Repeat;Synapse;Transit peptide;Zinc;Zinc-finger SUBCELLULAR LOCATION: Mitochondrion matrix. Cytoplasm, cytosol Postsynaptic cell membrane ; Peripheral membrane protein Note=Recruited to the postsynaptic cell membrane of the neuromuscular junction through interaction with MUSK. " Q96FC9,"PROTEIN NAMES: ATP-dependent DNA helicase DDX11 (CHL1-related protein 1) (hCHLR1) (DEAD/H-box protein 11) (Keratinocyte growth factor-regulated gene 2 protein) (KRG-2) PROTEIN FAMILY: DEAD box helicase family, DEAH subfamily, DDX11/CHL1 sub-subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the DEAD box helicase family. DEAH subfamily. DDX11/CHL1 sub-subfamily. FUNCTION: DNA-dependent ATPase and ATP-dependent DNA helicase that participates in various functions in genomic stability, including DNA replication, DNA repair and heterochromatin organization as well as in ribosomal RNA synthesis. Its double-stranded DNA helicase activity requires either a minimal 5'-single-stranded tail length of approximately 15 nt (flap substrates) or 10 nt length single-stranded gapped DNA substrates of a partial duplex DNA structure for helicase loading and translocation along DNA in a 5' to 3' direction. The helicase activity is capable of displacing duplex regions up to 100 bp, which can be extended up to 500 bp by the replication protein A (RPA) or the cohesion CTF18-replication factor C (Ctf18-RFC) complex activities. Shows also ATPase- and helicase activities on substrates that mimic key DNA intermediates of replication, repair and homologous recombination reactions, including forked duplex, anti-parallel G-quadruplex and three-stranded D-loop DNA molecules. Plays a role in DNA double-strand break (DSB) repair at the DNA replication fork during DNA replication recovery from DNA damage. Recruited with TIMELESS factor upon DNA-replication stress response at DNA replication fork to preserve replication fork progression, and hence ensure DNA replication fidelity. Cooperates also with TIMELESS factor during DNA replication to regulate proper sister chromatid cohesion and mitotic chromosome segregation. Stimulates 5'-single-stranded DNA flap endonuclease activity of FEN1 in an ATP- and helicase-independent manner; and hence it may contribute in Okazaki fragment processing at DNA replication fork during lagging strand DNA synthesis. Its ability to function at DNA replication fork is modulated by its binding to long non-coding RNA (lncRNA) cohesion regulator non-coding RNA DDX11-AS1/CONCR, which is able to increase both DDX11 ATPase activity and binding to DNA replicating regions. Also plays a role in heterochromatin organization. Involved in rRNA transcription activation through binding to active hypomethylated rDNA gene loci by recruiting UBTF and the RNA polymerase Pol I transcriptional machinery. Plays a role in embryonic development and prevention of aneuploidy (By similarity). Involved in melanoma cell proliferation and survival. Associates with chromatin at DNA replication fork regions. Binds to single- and double-stranded DNAs.; FUNCTION: (Microbial infection) Required for bovine papillomavirus type 1 regulatory protein E2 loading onto mitotic chromosomes during DNA replication for the viral genome to be maintained and segregated. KEYWORDS: 4Fe-4S;Activator;Alternative splicing;ATP-binding;Chromosome;Cytoplasm;Cytoskeleton;Developmental protein;Disease variant;DNA damage;DNA repair;DNA replication;DNA-binding;Helicase;Host-virus interaction;Hydrolase;Iron;Iron-sulfur;Metal-binding;Nucleotide-binding;Nucleus;Phosphoprotein;Reference proteome;RNA-binding;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus Nucleus, nucleolus Cytoplasm, cytoskeleton, spindle pole Midbody Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Note=During the early stages of mitosis, localizes to condensed chromatin and is released from the chromatin with progression to metaphase. Also localizes to the spindle poles throughout mitosis and at the midbody at later stages of mitosis (metaphase to telophase). In interphase, colocalizes with nucleolin in the nucleolus.; SUBCELLULAR LOCATION: Chromosome Note=(Microbial infection) Colocalizes with bovine papillomavirus type 1 regulatory protein E2 on mitotic chromosomes at early stages of mitosis. " Q96G46,"PROTEIN NAMES: tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like (mRNA-dihydrouridine synthase DUS3L) (tRNA-dihydrouridine synthase 3-like) PROTEIN FAMILY: Dus family, Dus3 subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the Dus family. Dus3 subfamily. FUNCTION: Catalyzes the synthesis of dihydrouridine, a modified base, in various RNAs, such as tRNAs, mRNAs and some long non-coding RNAs (lncRNAs). Mainly modifies the uridine in position 47 (U47) in the D-loop of most cytoplasmic tRNAs. Also able to mediate the formation of dihydrouridine in some mRNAs, thereby regulating their translation. KEYWORDS: Acetylation;Alternative splicing;Flavoprotein;FMN;Isopeptide bond;Metal-binding;mRNA processing;NAD;NADP;Oxidoreductase;Phosphoprotein;Reference proteome;Repeat;tRNA processing;Ubl conjugation;Zinc;Zinc-finger MISCELLANEOUS: [Isoform 2]: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay." Q96GJ1,"PROTEIN NAMES: tRNA (uracil-5-)-methyltransferase homolog B (TRM2 homolog B) (rRNA (uracil-5-)-methyltransferase TRMT2B) PROTEIN FAMILY: Class I-like SAM-binding methyltransferase superfamily, RNA M5U methyltransferase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family. FUNCTION: Mitochondrial S-adenosyl-L-methionine-dependent methyltransferase that catalyzes the formation of 5-methyl-uridine in tRNAs and 12S rRNA. Catalyzes the methylation of uridine at position 54 (m5U54) in all tRNAs. Specifically methylates the uridine in position 429 of 12S rRNA (m5U429). Does not affect RNA stability or mitochondrial translation. KEYWORDS: Alternative splicing;Methyltransferase;Mitochondrion;Reference proteome;rRNA processing;S-adenosyl-L-methionine;Transferase;Transit peptide;tRNA processing SUBCELLULAR LOCATION: Mitochondrion.; SUBCELLULAR LOCATION: [Isoform 1]: Mitochondrion matrix " Q96GN5,"PROTEIN NAMES: Cell division cycle-associated 7-like protein (Protein JPO2) (Transcription factor RAM2) ORGANISM: Homo sapiens (Human) FUNCTION: Plays a role in transcriptional regulation as a repressor that inhibits monoamine oxidase A (MAOA) activity and gene expression by binding to the promoter. Plays an important oncogenic role in mediating the full transforming effect of MYC in medulloblastoma cells. Involved in apoptotic signaling pathways; May act downstream of P38-kinase and BCL-2, but upstream of CASP3/caspase-3 as well as CCND1/cyclin D1 and E2F1. KEYWORDS: 3D-structure;Alternative splicing;Cytoplasm;Isopeptide bond;Nucleus;Phosphoprotein;Reference proteome;Repressor;Transcription;Transcription regulation;Ubl conjugation SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Associates with chromatin. Translocates from cytoplasm to nucleus under dexamethasone induction. MISCELLANEOUS: Cells lacking CDCA7L display a reduction of 25-30% of colony formation in medulloblastoma cell lines. CDCA7L overexpression induces colony formation." Q96GQ7,"PROTEIN NAMES: Probable ATP-dependent RNA helicase DDX27 (DEAD box protein 27) PROTEIN FAMILY: DEAD box helicase family, DDX27/DRS1 subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the DEAD box helicase family. DDX27/DRS1 subfamily. FUNCTION: Probable ATP-dependent RNA helicase. Component of the nucleolar ribosomal RNA (rRNA) processing machinery that regulates 3' end formation of ribosomal 47S rRNA. KEYWORDS: ATP-binding;Chromosome;Helicase;Hydrolase;Nucleotide-binding;Nucleus;Phosphoprotein;Reference proteome;Ribosome biogenesis;rRNA processing SUBCELLULAR LOCATION: Nucleus, nucleolus Chromosome Note=Associates with 60S and 90S pre-ribosomal particles. " Q96H15,"PROTEIN NAMES: T-cell immunoglobulin and mucin domain-containing protein 4 (TIMD-4) (T-cell immunoglobulin mucin receptor 4) (TIM-4) (T-cell membrane protein 4) PROTEIN FAMILY: Immunoglobulin superfamily, TIM family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the immunoglobulin superfamily. TIM family. FUNCTION: Phosphatidylserine receptor that plays different role in immune response including phagocytosis of apoptotic cells and T-cell regulation. Controls T-cell activation in a bimodal fashion, decreasing the activation of naive T-cells by inducing cell cycle arrest, while increasing proliferation of activated T-cells by activating AKT1 and ERK1/2 phosphorylations and subsequent signaling pathways (By similarity). Also plays a role in efferocytosis which is the process by which apoptotic cells are removed by phagocytic cells. Mechanistically, promotes the engulfment of apoptotic cells or exogenous particles by securing them to phagocytes through direct binding to phosphatidylserine present on apoptotic cells, while other engulfment receptors such as MERTK efficiently recognize apoptotic cells and mediate their ingestion. Additionally, promotes autophagy process by suppressing NLRP3 inflammasome activity via activation of LKB1/PRKAA1 pathway in a phosphatidylserine-dependent mechanism (By similarity).; FUNCTION: (Microbial infection) Plays a positive role in exosome-mediated trafficking of HIV-1 virus and its entry into immune cells. KEYWORDS: 3D-structure;Alternative splicing;Cell membrane;Disulfide bond;Glycoprotein;Immunoglobulin domain;Membrane;Phosphoprotein;Reference proteome;Secreted;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein Secreted, extracellular exosome " Q96H86,"PROTEIN NAMES: Zinc finger protein 764 PROTEIN FAMILY: Krueppel C2H2-type zinc-finger protein family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein family. FUNCTION: Zinc finger protein that functions as a cofactor for steroid hormone receptors, such as NR3C1/GR. Directs NR3C1/GR transcriptional activity toward specific biologic pathways by changing NR3C1/GR binding and transcriptional activity on the glucocorticoid-responsive genes. KEYWORDS: Alternative splicing;DNA-binding;Metal-binding;Nucleus;Reference proteome;Repeat;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus " Q96I34,"PROTEIN NAMES: Protein phosphatase 1 regulatory subunit 16A (Myosin phosphatase-targeting subunit 3) ORGANISM: Homo sapiens (Human) FUNCTION: Inhibits protein phosphatase 1 activity toward phosphorylase, myosin light chain and myosin substrates. KEYWORDS: ANK repeat;Cell membrane;Coiled coil;Lipoprotein;Membrane;Methylation;Palmitate;Phosphoprotein;Prenylation;Reference proteome;Repeat SUBCELLULAR LOCATION: Cell membrane ; Lipid-anchor " Q96JB8,"PROTEIN NAMES: MAGUK p55 subfamily member 4 (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 5 protein) (Discs large homolog 6) PROTEIN FAMILY: MAGUK family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the MAGUK family. FUNCTION: May play a role in retinal photoreceptors development. KEYWORDS: Alternative splicing;Coiled coil;Cytoplasm;Reference proteome;Repeat;SH3 domain SUBCELLULAR LOCATION: Cytoplasm Note=Detected at the outer limiting membrane (OLM) and in the outer plexiform layer (OPL) of the retina. At the OLM, detected apical to the adherens junction (AJ). " Q96JY6,"PROTEIN NAMES: PDZ and LIM domain protein 2 (PDZ-LIM protein mystique) ORGANISM: Homo sapiens (Human) FUNCTION: Probable adapter protein located at the actin cytoskeleton that promotes cell attachment. Necessary for the migratory capacity of epithelial cells. Overexpression enhances cell adhesion to collagen and fibronectin and suppresses anchorage independent growth. May contribute to tumor cell migratory capacity. KEYWORDS: 3D-structure;Alternative splicing;Cytoplasm;Cytoskeleton;LIM domain;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Zinc SUBCELLULAR LOCATION: Cytoplasm Nucleus Note=May be partially nuclear.; SUBCELLULAR LOCATION: [Isoform 1]: Cytoplasm, cytoskeleton. Note=Colocalizes with beta-1 integrin (ITGB1) and alpha-actinin but not with paxillin (PXN).; SUBCELLULAR LOCATION: [Isoform 2]: Cytoplasm, cytoskeleton.; SUBCELLULAR LOCATION: [Isoform 3]: Nucleus. " Q96K21,"PROTEIN NAMES: Abscission/NoCut checkpoint regulator (ANCHR) (MLL partner containing FYVE domain) (Zinc finger FYVE domain-containing protein 19) ORGANISM: Homo sapiens (Human) FUNCTION: Key regulator of abscission step in cytokinesis: part of the cytokinesis checkpoint, a process required to delay abscission to prevent both premature resolution of intercellular chromosome bridges and accumulation of DNA damage. Together with CHMP4C, required to retain abscission-competent VPS4 (VPS4A and/or VPS4B) at the midbody ring until abscission checkpoint signaling is terminated at late cytokinesis. Deactivation of AURKB results in dephosphorylation of CHMP4C followed by its dissociation from ZFYVE19/ANCHR and VPS4 and subsequent abscission. KEYWORDS: Alternative splicing;Cell cycle;Cell division;Chromosomal rearrangement;Ciliopathy;Coiled coil;Cytoplasm;Cytoskeleton;Disease variant;Intrahepatic cholestasis;Isopeptide bond;Lipid-binding;Metal-binding;Phosphoprotein;Proto-oncogene;Reference proteome;Ubl conjugation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Cleavage furrow Midbody, Midbody ring Note=Localizes mainly on centrosomes in interphase and early mitosis. Localizes at the cleavage furrow and midbody ring in late mitosis and cytokinesis. " Q96KN2,"PROTEIN NAMES: Beta-Ala-His dipeptidase (CNDP dipeptidase 1) (Carnosine dipeptidase 1) (Glutamate carboxypeptidase-like protein 2) (Serum carnosinase) PROTEIN FAMILY: Peptidase M20A family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the peptidase M20A family. FUNCTION: Catalyzes the peptide bond hydrolysis in Xaa-His dipeptides, displaying the highest activity toward carnosine (beta-alanyl-L-histidine) and anserine (beta-alanyl-3-methyl-histidine). KEYWORDS: 3D-structure;Carboxypeptidase;Glycoprotein;Hydrolase;Metal-binding;Metalloprotease;Phosphoprotein;Protease;Reference proteome;Secreted;Signal;Zinc SUBCELLULAR LOCATION: Secreted " Q96KR6,"PROTEIN NAMES: Protein FAM210B, mitochondrial PROTEIN FAMILY: FAM210 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the FAM210 family. FUNCTION: Plays a role in erythroid differentiation. Involved in cell proliferation and tumor cell growth suppression. Involved in the metabolic reprogramming of cancer cells in a PDK4-dependent manner. KEYWORDS: Differentiation;Erythrocyte maturation;Membrane;Mitochondrion;Mitochondrion outer membrane;Reference proteome;Transit peptide;Transmembrane;Transmembrane helix;Tumor suppressor SUBCELLULAR LOCATION: Mitochondrion Mitochondrion outer membrane ; Multi-pass membrane protein " Q96L58,"PROTEIN NAMES: Beta-1,3-galactosyltransferase 6 (Beta-1,3-GalTase 6) (Beta3Gal-T6) (Beta3GalT6) (GAG GalTII) (Galactosyltransferase II) (Galactosylxylosylprotein 3-beta-galactosyltransferase) (UDP-Gal:betaGal beta 1,3-galactosyltransferase polypeptide 6) PROTEIN FAMILY: Glycosyltransferase 31 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the glycosyltransferase 31 family. FUNCTION: Beta-1,3-galactosyltransferase that transfers galactose from UDP-galactose to substrates with a terminal beta-linked galactose residue. Has a preference for galactose-beta-1,4-xylose that is found in the linker region of glycosaminoglycans, such as heparan sulfate and chondroitin sulfate. Has no activity towards substrates with terminal glucosamine or galactosamine residues. PATHWAY: Glycan metabolism; chondroitin sulfate biosynthesis.; PATHWAY: Glycan metabolism; heparan sulfate biosynthesis. KEYWORDS: Disease variant;Dwarfism;Ehlers-Danlos syndrome;Glycoprotein;Glycosyltransferase;Golgi apparatus;Manganese;Membrane;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus, Golgi stack membrane ; Single-pass type II membrane protein " Q96LA5,PROTEIN NAMES: Fc receptor-like protein 2 (FcR-like protein 2) (FcRL2) (Fc receptor homolog 2) (FcRH2) (IFGP family protein 4) (Immunoglobulin receptor translocation-associated protein 4) (SH2 domain-containing phosphatase anchor protein 1) (CD antigen CD307b) ORGANISM: Homo sapiens (Human) FUNCTION: May have an regulatory role in normal and neoplastic B cell development. KEYWORDS: Alternative splicing;Cell membrane;Direct protein sequencing;Disulfide bond;Glycoprotein;Immunoglobulin domain;Membrane;Receptor;Reference proteome;Repeat;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein Q96LJ7,"PROTEIN NAMES: Dehydrogenase/reductase SDR family member 1 (Short chain dehydrogenase/reductase family 19C member 1) (Protein SDR19C1) PROTEIN FAMILY: Short-chain dehydrogenases/reductases (SDR) family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FUNCTION: NADPH-dependent oxidoreductase which catalyzes the reduction of steroids (estrone, androstene-3,17-dione and cortisone) as well as prostaglandin E1, isatin and xenobiotics in vitro. May have a role in steroid and/or xenobiotic metabolism. KEYWORDS: 3D-structure;Acetylation;Endoplasmic reticulum;Methylation;NAD;Oxidoreductase;Reference proteome SUBCELLULAR LOCATION: Endoplasmic reticulum Note=May be attached to the ER membrane by its C-terminus segment. " Q96LL9,"PROTEIN NAMES: DnaJ homolog subfamily C member 30, mitochondrial (Williams-Beuren syndrome chromosomal region 18 protein) ORGANISM: Homo sapiens (Human) FUNCTION: Mitochondrial protein enriched in neurons that acts as a regulator of mitochondrial respiration (By similarity). Associates with the ATP synthase complex and facilitates ATP synthesis (By similarity). May be a chaperone protein involved in the turnover of the subunits of mitochondrial complex I N-module. It facilitates the degradation of N-module subunits damaged by oxidative stress, and contributes to complex I functional efficiency. KEYWORDS: 3D-structure;ATP synthesis;Chaperone;Disease variant;Leber hereditary optic neuropathy;Membrane;Mitochondrion;Mitochondrion inner membrane;Primary mitochondrial disease;Reference proteome;Transit peptide;Transmembrane;Transmembrane helix;Williams-Beuren syndrome SUBCELLULAR LOCATION: Mitochondrion inner membrane ; Single-pass membrane protein " Q96LX8,PROTEIN NAMES: Zinc finger protein 597 PROTEIN FAMILY: Krueppel C2H2-type zinc-finger protein family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein family. FUNCTION: May be involved in transcriptional regulation. KEYWORDS: DNA-binding;Isopeptide bond;Metal-binding;Nucleus;Reference proteome;Repeat;Transcription;Transcription regulation;Ubl conjugation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Q96M27,PROTEIN NAMES: Protein PRRC1 (Proline-rich and coiled-coil-containing protein 1) PROTEIN FAMILY: PRRC1 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the PRRC1 family. FUNCTION: May act as a regulator of the protein kinase A (PKA) activity during embryonic development. KEYWORDS: Alternative splicing;Cytoplasm;Golgi apparatus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Golgi apparatus Cytoplasm MISCELLANEOUS: [Isoform 2]: Non-canonical splice sites for exon 9 and exon 10. Q96M32,"PROTEIN NAMES: Adenylate kinase 7 (AK 7) (ATP-AMP transphosphorylase 7) PROTEIN FAMILY: Adenylate kinase family; Dpy-30 family ORGANISM: Homo sapiens (Human) SIMILARITY: In the central section; belongs to the adenylate kinase family.; SIMILARITY: In the C-terminal section; belongs to the dpy-30 family. FUNCTION: Nucleoside monophosphate (NMP) kinase that catalyzes the reversible transfer of the terminal phosphate group between nucleoside triphosphates and monophosphates. Has highest activity toward AMP, and weaker activity toward dAMP, CMP and dCMP. Also displays broad nucleoside diphosphate kinase activity. Involved in maintaining ciliary structure and function. KEYWORDS: ATP-binding;Cell projection;Cilium;Cilium biogenesis/degradation;Coiled coil;Cytoplasm;Disease variant;Flagellum;Kinase;Nucleotide-binding;Reference proteome;Transferase SUBCELLULAR LOCATION: Cytoplasm, cytosol Cell projection, cilium, flagellum Note=Detected along the full length of sperm flagellum, where it colocalizes with alpha-tubulin. " Q96MF2,"PROTEIN NAMES: SH3 and cysteine-rich domain-containing protein 3 ORGANISM: Homo sapiens (Human) FUNCTION: Required for normal excitation-contraction coupling in skeletal muscle and for normal muscle contraction in response to membrane depolarization. Required for normal Ca(2+) release from the sarcplasmic reticulum, which ultimately leads to muscle contraction. Probably functions via its effects on muscle calcium channels. Increases CACNA1S channel activity, in addition to its role in enhancing the expression of CACNA1S at the cell membrane. Has a redundant role in promoting the expression of the calcium channel CACNA1S at the cell membrane (By similarity). Slows down the inactivation rate of the calcium channel CACNA1C. KEYWORDS: 3D-structure;Alternative splicing;Cell membrane;Cytoplasm;Disease variant;Membrane;Metal-binding;Reference proteome;Repeat;SH3 domain;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cytoplasm Cell membrane, sarcolemma ; Peripheral membrane protein ; Cytoplasmic side Cell membrane, sarcolemma, T-tubule Note=Co-localizes with CACNA1S and CACNA1C on T-tubules. " Q96MM3,"PROTEIN NAMES: Zinc finger protein 42 homolog (Zfp-42) (Reduced expression protein 1) (REX-1) (hREX-1) (Zinc finger protein 754) PROTEIN FAMILY: Krueppel C2H2-type zinc-finger protein family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein family. FUNCTION: Involved in the reprogramming of X-chromosome inactivation during the acquisition of pluripotency. Required for efficient elongation of TSIX, a non-coding RNA antisense to XIST. Binds DXPas34 enhancer within the TSIX promoter. Involved in ES cell self-renewal (By similarity). KEYWORDS: Activator;DNA-binding;Isopeptide bond;Metal-binding;Nucleus;Reference proteome;Repeat;Transcription;Transcription regulation;Ubl conjugation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus " Q96N19,PROTEIN NAMES: Integral membrane protein GPR137 (Transmembrane 7 superfamily member 1-like 1 protein) PROTEIN FAMILY: GPR137 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the GPR137 family. FUNCTION: Lysosomal integral membrane protein that may regulate MTORC1 complex translocation to lysosomes. May play a role in autophagy.; FUNCTION: May activate Wnt/beta-catenin signaling to modulate epithelial cell function. KEYWORDS: Alternative splicing;Autophagy;Glycoprotein;Lysosome;Membrane;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Lysosome membrane ; Multi-pass membrane protein Q96N77,PROTEIN NAMES: Zinc finger protein 641 PROTEIN FAMILY: Krueppel C2H2-type zinc-finger protein family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein family. FUNCTION: Transcriptional activator. Activates transcriptional activities of SRE and AP-1. KEYWORDS: Alternative splicing;DNA-binding;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Repeat;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Q96NC0,PROTEIN NAMES: Zinc finger matrin-type protein 2 ORGANISM: Homo sapiens (Human) FUNCTION: Involved in pre-mRNA splicing as a component of the spliceosome. KEYWORDS: 3D-structure;Acetylation;DNA-binding;Isopeptide bond;Metal-binding;mRNA processing;mRNA splicing;Nucleus;Reference proteome;Spliceosome;Ubl conjugation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Q96NG3,"PROTEIN NAMES: Outer dynein arm-docking complex subunit 4 (Tetratricopeptide repeat protein 25) (TPR repeat protein 25) ORGANISM: Homo sapiens (Human) FUNCTION: Component of the outer dynein arm-docking complex (ODA-DC) that mediates outer dynein arms (ODA) binding onto the doublet microtubule. Plays an essential role for the assembly of ODA-DC and for the docking of ODA in ciliary axoneme. KEYWORDS: 3D-structure;Alternative splicing;Cell projection;Ciliopathy;Cytoplasm;Cytoskeleton;Kartagener syndrome;Primary ciliary dyskinesia;Reference proteome;Repeat;TPR repeat SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, cilium axoneme " Q96P15,"PROTEIN NAMES: Serpin B11 PROTEIN FAMILY: Serpin family, Ov-serpin subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the serpin family. Ov-serpin subfamily. FUNCTION: Has no serine protease inhibitory activity, probably due to variants in the scaffold impairing conformational change. KEYWORDS: Alternative splicing;Cytoplasm;Protease inhibitor;Reference proteome;Serine protease inhibitor SUBCELLULAR LOCATION: Cytoplasm " Q96P71,"PROTEIN NAMES: N-terminal EF-hand calcium-binding protein 3 (Amyloid-beta A4 protein-binding family A member 2-binding protein) (Nek2-interacting protein 1) (Neuronal calcium-binding protein 3) (X11L-binding protein 51) ORGANISM: Homo sapiens (Human) FUNCTION: Inhibits the interaction of APBA2 with amyloid-beta precursor protein (APP), and hence allows formation of amyloid-beta. May enhance the activity of HIF1A and thus promote glycolysis under normoxic conditions; the function requires its ABM domain and may implicate the stabilization of the interaction between HIF1AN and APBA3. KEYWORDS: Alternative splicing;Calcium;Golgi apparatus;Metal-binding;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Golgi apparatus MISCELLANEOUS: [Isoform 3]: May result from the retention of an intron in the cDNA." Q96PJ5,PROTEIN NAMES: Fc receptor-like protein 4 (FcR-like protein 4) (FcRL4) (Fc receptor homolog 4) (FcRH4) (IFGP family protein 2) (hIFGP2) (Immune receptor translocation-associated protein 1) (CD antigen CD307d) ORGANISM: Homo sapiens (Human) FUNCTION: May function as an inhibitor of the B-cell receptor signaling. May function in the B-cell-mediated immune response. KEYWORDS: Adaptive immunity;Alternative splicing;Cell membrane;Chromosomal rearrangement;Disulfide bond;Glycoprotein;Immunity;Immunoglobulin domain;Membrane;Phosphoprotein;Receptor;Reference proteome;Repeat;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein Q96Q40,"PROTEIN NAMES: Cyclin-dependent kinase 15 (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 7 protein) (Cell division protein kinase 15) (Serine/threonine-protein kinase ALS2CR7) (Serine/threonine-protein kinase PFTAIRE-2) PROTEIN FAMILY: Protein kinase superfamily, CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. FUNCTION: Serine/threonine-protein kinase that acts like an antiapoptotic protein that counters TRAIL/TNFSF10-induced apoptosis by inducing phosphorylation of BIRC5 at 'Thr-34'. KEYWORDS: Alternative splicing;ATP-binding;Kinase;Magnesium;Metal-binding;Nucleotide-binding;Reference proteome;Serine/threonine-protein kinase;Transferase MISCELLANEOUS: [Isoform 5]: May be due to competing acceptor splice site." Q96RD9,PROTEIN NAMES: Fc receptor-like protein 5 (FcR-like protein 5) (FcRL5) (BXMAS1) (Fc receptor homolog 5) (FcRH5) (Immune receptor translocation-associated protein 2) (CD antigen CD307e) ORGANISM: Homo sapiens (Human) FUNCTION: May be involved in B-cell development and differentiation in peripheral lymphoid organs and may be useful markers of B-cell stages. May have an immunoregulatory role in marginal zone B-cells. May play a role in fertilization (By similarity). KEYWORDS: Alternative splicing;Cell membrane;Disulfide bond;Fertilization;Glycoprotein;Immunoglobulin domain;Membrane;Receptor;Reference proteome;Repeat;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein Q96RL6,"PROTEIN NAMES: Sialic acid-binding Ig-like lectin 11 (Sialic acid-binding lectin 11) (Siglec-11) PROTEIN FAMILY: Immunoglobulin superfamily, SIGLEC (sialic acid binding Ig-like lectin) family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family. FUNCTION: Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,8-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. KEYWORDS: Alternative splicing;Cell adhesion;Disulfide bond;Glycoprotein;Immunoglobulin domain;Lectin;Membrane;Phosphoprotein;Reference proteome;Repeat;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. " Q96RP7,"PROTEIN NAMES: Galactose-3-O-sulfotransferase 4 (Gal3ST-4) (Beta-galactose-3-O-sulfotransferase 4) (Gal-beta-1,3-GalNAc 3'-sulfotransferase) PROTEIN FAMILY: Galactose-3-O-sulfotransferase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the galactose-3-O-sulfotransferase family. FUNCTION: Catalyzes the transfer of sulfate to beta-1,3-linked galactose residues in O-linked glycoproteins. Good substrates include asialofetuin, Gal-beta-1,3-GalNAc and Gal-beta-1,3 (GlcNAc-beta-1,6)GalNAc. PATHWAY: Protein modification; carbohydrate sulfation. KEYWORDS: Alternative splicing;Glycoprotein;Golgi apparatus;Membrane;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus, Golgi stack membrane ; Single-pass type II membrane protein " Q96RQ3,"PROTEIN NAMES: Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial (MCCase subunit alpha) (3-methylcrotonyl-CoA carboxylase 1) (3-methylcrotonyl-CoA carboxylase biotin-containing subunit) (3-methylcrotonyl-CoA:carbon dioxide ligase subunit alpha) ORGANISM: Homo sapiens (Human) FUNCTION: Biotin-attachment subunit of the 3-methylcrotonyl-CoA carboxylase, an enzyme that catalyzes the conversion of 3-methylcrotonyl-CoA to 3-methylglutaconyl-CoA, a critical step for leucine and isovaleric acid catabolism. PATHWAY: Amino-acid degradation; L-leucine degradation; (S)-3-hydroxy-3-methylglutaryl-CoA from 3-isovaleryl-CoA: step 2/3. KEYWORDS: 3D-structure;Acetylation;ATP-binding;Biotin;Direct protein sequencing;Disease variant;Ligase;Mitochondrion;Nucleotide-binding;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Mitochondrion matrix " Q96S59,"PROTEIN NAMES: Ran-binding protein 9 (RanBP9) (BPM-L) (BPM90) (Ran-binding protein M) (RanBPM) (RanBP7) PROTEIN FAMILY: RANBP9/10 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the RANBP9/10 family. FUNCTION: May act as scaffolding protein, and as adapter protein to couple membrane receptors to intracellular signaling pathways (Probable). Acts as a mediator of cell spreading and actin cytoskeleton rearrangement. Core component of the CTLH E3 ubiquitin-protein ligase complex that selectively accepts ubiquitin from UBE2H and mediates ubiquitination and subsequent proteasomal degradation of the transcription factor HBP1. May be involved in signaling of ITGB2/LFA-1 and other integrins. Enhances HGF-MET signaling by recruiting Sos and activating the Ras pathway. Enhances dihydrotestosterone-induced transactivation activity of AR, as well as dexamethasone-induced transactivation activity of NR3C1, but not affect estrogen-induced transactivation. Stabilizes TP73 isoform Alpha, probably by inhibiting its ubiquitination, and increases its proapoptotic activity. Inhibits the kinase activity of DYRK1A and DYRK1B. Inhibits FMR1 binding to RNA. KEYWORDS: 3D-structure;Acetylation;Alternative splicing;Cell membrane;Cytoplasm;Membrane;Nucleus;Phosphoprotein;Reference proteome;Ubl conjugation SUBCELLULAR LOCATION: Cytoplasm Nucleus Cell membrane ; Peripheral membrane protein Note=The unphosphorylated form is predominantly cytoplasmic. A phosphorylated form is associated with the plasma membrane. " Q96SC8,PROTEIN NAMES: Doublesex- and mab-3-related transcription factor A2 (Doublesex- and mab-3-related transcription factor 5) PROTEIN FAMILY: DMRT family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the DMRT family. FUNCTION: May be involved in sexual development. KEYWORDS: DNA-binding;Metal-binding;Nucleus;Reference proteome;Zinc SUBCELLULAR LOCATION: Nucleus Q96SZ5,PROTEIN NAMES: 2-aminoethanethiol dioxygenase (Cysteamine dioxygenase) ORGANISM: Homo sapiens (Human) FUNCTION: Plays a vital role in regulating thiol metabolism and preserving oxygen homeostasis by oxidizing the sulfur of cysteamine and N-terminal cysteine-containing proteins to their corresponding sulfinic acids using O2 as a cosubstrate. Catalyzes the oxidation of cysteamine (2-aminoethanethiol) to hypotaurine. Catalyzes the oxidation of regulators of G-protein signaling 4 (RGS4) and 5 (RGS5) and interleukin-32 (IL32). KEYWORDS: 3D-structure;Dioxygenase;Iron;Metal-binding;Oxidoreductase;Reference proteome;Thioether bond Q96T21,"PROTEIN NAMES: Selenocysteine insertion sequence-binding protein 2 (SECIS-binding protein 2) ORGANISM: Homo sapiens (Human) FUNCTION: mRNA-binding protein that binds to the SECIS (selenocysteine insertion sequence) element present in the 3'-UTR of mRNAs encoding selenoproteins and facilitates the incorporation of the rare amino acid selenocysteine. Insertion of selenocysteine at UGA codons is mediated by SECISBP2 and EEFSEC: SECISBP2 (1) specifically binds the SECIS sequence once the 80S ribosome encounters an in-frame UGA codon and (2) contacts the RPS27A/eS31 of the 40S ribosome before ribosome stalling. (3) GTP-bound EEFSEC then delivers selenocysteinyl-tRNA(Sec) to the 80S ribosome and adopts a preaccommodated state conformation. (4) After GTP hydrolysis, EEFSEC dissociates from the assembly, selenocysteinyl-tRNA(Sec) accommodates, and peptide bond synthesis and selenoprotein elongation occur. KEYWORDS: 3D-structure;Alternative splicing;Disease variant;Mitochondrion;Nucleus;Protein biosynthesis;Reference proteome;RNA-binding;Transit peptide SUBCELLULAR LOCATION: [Isoform 1]: Nucleus.; SUBCELLULAR LOCATION: [Isoform 2]: Mitochondrion MISCELLANEOUS: [Isoform 2]: Contains a transit peptide at positions 1-15." Q96T55,"PROTEIN NAMES: Potassium channel subfamily K member 16 (2P domain potassium channel Talk-1) (TWIK-related alkaline pH-activated K(+) channel 1) (TALK-1) PROTEIN FAMILY: Two pore domain potassium channel family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the two pore domain potassium channel (TC 1.A.1.8) family. FUNCTION: Outward rectifying potassium channel. Produces rapidly activating and non-inactivating outward rectifier K(+) currents. KEYWORDS: Alternative splicing;Ion channel;Ion transport;Membrane;Potassium;Potassium channel;Potassium transport;Reference proteome;Transmembrane;Transmembrane helix;Transport;Voltage-gated channel SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. MISCELLANEOUS: Inhibited by Ba(2+), quinine, quinidine, chloroform and halothane. Activated at alkaline pH." Q96T68,"PROTEIN NAMES: Histone-lysine N-methyltransferase SETDB2 (Chronic lymphocytic leukemia deletion region gene 8 protein) (Lysine N-methyltransferase 1F) (SET domain bifurcated 2) PROTEIN FAMILY: Class V-like SAM-binding methyltransferase superfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the class V-like SAM-binding methyltransferase superfamily. FUNCTION: Histone methyltransferase involved in left-right axis specification in early development and mitosis. Specifically trimethylates 'Lys-9' of histone H3 (H3K9me3). H3K9me3 is a specific tag for epigenetic transcriptional repression that recruits HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Contributes to H3K9me3 in both the interspersed repetitive elements and centromere-associated repeats. Plays a role in chromosome condensation and segregation during mitosis. KEYWORDS: 3D-structure;Alternative splicing;Cell cycle;Cell division;Chromatin regulator;Chromosome;Developmental protein;Metal-binding;Methyltransferase;Mitosis;Nucleus;Reference proteome;S-adenosyl-L-methionine;Transferase;Zinc SUBCELLULAR LOCATION: Nucleus Chromosome " Q96WV9,"PROTEIN NAMES: Probable cyclin-dependent kinase 9 (Cell division protein kinase 9) PROTEIN FAMILY: Protein kinase superfamily, CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. FUNCTION: Component of the positive transcription elongation factor b (P-TEFb) which consists of cdk9 and pch1, and which phosphorylates the C-terminal domain (CTD) of RNA polymerase II and spt5. KEYWORDS: ATP-binding;Kinase;Nucleotide-binding;Nucleus;Phosphoprotein;Reference proteome;Serine/threonine-protein kinase;Transcription;Transcription regulation;Transferase SUBCELLULAR LOCATION: Nucleus. " Q96YK1,"PROTEIN NAMES: Malonyl-CoA reductase PROTEIN FAMILY: Aspartate-semialdehyde dehydrogenase family ORGANISM: Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) SIMILARITY: Belongs to the aspartate-semialdehyde dehydrogenase family. FUNCTION: Catalyzes the reduction of malonyl-CoA to malonate semialdehyde, a key step in the 3-hydroxypropanoate and the 3-hydroxypropanoate/4-hydroxybutyrate cycles. Can also use succinyl-CoA and succinate semialdehyde as substrates but at a lower rate than malonyl-CoA. KEYWORDS: 3D-structure;NADP;Oxidoreductase;Reference proteome;RNA-binding MISCELLANEOUS: This enzyme contains bound RNA, but the physiological role is not known." Q970S6,"PROTEIN NAMES: Threonylcarbamoyl-AMP synthase (TC-AMP synthase) (L-threonylcarbamoyladenylate synthase) (t(6)A37 threonylcarbamoyladenosine biosynthesis protein Sua5) (tRNA threonylcarbamoyladenosine biosynthesis protein Sua5) PROTEIN FAMILY: SUA5 family ORGANISM: Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) SIMILARITY: Belongs to the SUA5 family. FUNCTION: Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine (By similarity). Probably catalyzes the conversion of L-threonine, HCO(3)(-)/CO(2) and ATP to give threonylcarbamoyl-AMP (TC-AMP) as the acyladenylate intermediate, with the release of diphosphate. Shows ATP hydrolysis activity in vitro, producing AMP. KEYWORDS: 3D-structure;ATP-binding;Cytoplasm;Direct protein sequencing;Nucleotide-binding;Nucleotidyltransferase;Reference proteome;Transferase;tRNA processing SUBCELLULAR LOCATION: Cytoplasm " Q972D2,"PROTEIN NAMES: L-aspartate oxidase (LAO) (LASPO) (Quinolinate synthase B) PROTEIN FAMILY: FAD-dependent oxidoreductase 2 family, NadB subfamily ORGANISM: Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) SIMILARITY: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily. FUNCTION: Catalyzes the oxidation of L-aspartate to iminoaspartate, the first step in the de novo biosynthesis of NAD(+). Can also use L-asparagine, but not L-phenylalanine, L-glutamate, glycine, L-proline, L-alanine and D-aspartate. PATHWAY: Cofactor biosynthesis; NAD(+) biosynthesis; iminoaspartate from L-aspartate (oxidase route): step 1/1. KEYWORDS: 3D-structure;Cytoplasm;FAD;Flavoprotein;Nucleotide-binding;Oxidoreductase;Pyridine nucleotide biosynthesis;Reference proteome SUBCELLULAR LOCATION: Cytoplasm " Q97TX9,"PROTEIN NAMES: CRISPR-associated exonuclease Cas4 PROTEIN FAMILY: CRISPR-associated exonuclease Cas4 family ORGANISM: Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus) SIMILARITY: Belongs to the CRISPR-associated exonuclease Cas4 family. FUNCTION: CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (By similarity). This protein is a 5' to 3' partially processive exonuclease that cleaves off single mononucleotides. Has a marked preference for ssDNA, although in vitro it also acts on dsDNA and ssRNA. Has low endonuclease activity with circular ssDNA. Binds ssDNA and can unwind dsDNA; unwinding does not require ATP. KEYWORDS: 3D-structure;4Fe-4S;Antiviral defense;DNA-binding;Exonuclease;Hydrolase;Iron;Iron-sulfur;Manganese;Metal-binding;Nuclease;Reference proteome " Q97U96,PROTEIN NAMES: Arabinonate dehydratase PROTEIN FAMILY: Mandelate racemase/muconate lactonizing enzyme family ORGANISM: Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus) SIMILARITY: Belongs to the mandelate racemase/muconate lactonizing enzyme family. FUNCTION: Catalyzes the dehydration of D-arabinonate to 2-keto-3-deoxy-D-arabinonate. Participates in a pentose oxidation pathway that converts D-arabinonate to 2-oxoglutarate. KEYWORDS: Carbohydrate metabolism;Lyase;Magnesium;Metal-binding;Reference proteome Q97VT7,"PROTEIN NAMES: Aryldialkylphosphatase (Paraoxonase) (SsoPox) (Phosphotriesterase-like lactonase) PROTEIN FAMILY: Metallo-dependent hydrolases superfamily, Phosphotriesterase family ORGANISM: Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus) SIMILARITY: Belongs to the metallo-dependent hydrolases superfamily. Phosphotriesterase family. FUNCTION: Has a low paraoxonase activity. Also active, but with a lower activity, against other organo-phosphorus insecticides such as Dursban, Coumaphos, pNP-butanoate or parathion. KEYWORDS: 3D-structure;Cobalt;Direct protein sequencing;Hydrolase;Iron;Metal-binding;Reference proteome " Q97WG8,PROTEIN NAMES: DNA double-strand break repair helicase HerA PROTEIN FAMILY: HerA family ORGANISM: Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus) SIMILARITY: Belongs to the HerA family. FUNCTION: Involved in DNA double-strand break (DSB) repair. Acts probably with NurA to stimulate resection of the 5' strand and produce the long 3' single-strand that is required for RadA loading (By similarity). Exhibits DNA-dependent ATPase activity and DNA helicase activity. KEYWORDS: 3D-structure;ATP-binding;DNA damage;DNA repair;Helicase;Hydrolase;Nucleotide-binding;Reference proteome Q97YD4,"PROTEIN NAMES: CRISPR-associated exonuclease Csa1 PROTEIN FAMILY: CRISPR-associated protein Csa1 family ORGANISM: Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus) SIMILARITY: Belongs to the CRISPR-associated protein Csa1 family. FUNCTION: CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (By similarity). A ssDNA exonuclease that has both 5' to 3' and 3' to 5' activity, yielding 5'-OH and 3'-phosphate groups. Has Mn(2+)-dependent endonuclease activity on circular ssDNA. Can unwind dsDNA; unwinding does not require ATP. KEYWORDS: 4Fe-4S;Antiviral defense;Exonuclease;Hydrolase;Iron;Iron-sulfur;Manganese;Metal-binding;Nuclease;Reference proteome " Q988B8,PROTEIN NAMES: Pyridoxamine--pyruvate transaminase (Pyridoxamine-pyruvate aminotransferase) PROTEIN FAMILY: Class-V pyridoxal-phosphate-dependent aminotransferase family ORGANISM: Mesorhizobium japonicum (strain LMG 29417 / CECT 9101 / MAFF 303099) (Mesorhizobium loti (strain MAFF 303099)) SIMILARITY: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. FUNCTION: Catalyzes a reversible transamination reaction between pyridoxamine and pyruvate to form pyridoxal and L-alanine. KEYWORDS: 3D-structure;Aminotransferase;Chloride;Direct protein sequencing;Pyridoxal phosphate;Transferase Q988B9,"PROTEIN NAMES: 4-pyridoxolactonase PROTEIN FAMILY: Metallo-beta-lactamase superfamily ORGANISM: Mesorhizobium japonicum (strain LMG 29417 / CECT 9101 / MAFF 303099) (Mesorhizobium loti (strain MAFF 303099)) SIMILARITY: Belongs to the metallo-beta-lactamase superfamily. FUNCTION: Involved in the degradation of pyridoxine or pyridoxamine (free, phosphate-unbound, forms of vitamin B6). Hydrolyzes 4-pyridoxolactone to 4-pyridoxic acid. Has lower activity toward N-hexanoyl-D,L-homoserine lactone, but is not active toward 5-pyridoxolactone and gamma-butyrolactone. PATHWAY: Cofactor degradation; B6 vitamer degradation; 4-pyridoxate from pyridoxal: step 2/2. KEYWORDS: 3D-structure;Direct protein sequencing;Hydrolase;Metal-binding;Zinc " Q988C8,"PROTEIN NAMES: 5-formyl-3-hydroxy-2-methylpyridine 4-carboxylate 5-dehydrogenase (FHMPC dehydrogenase) PROTEIN FAMILY: 3-hydroxyacyl-CoA dehydrogenase family ORGANISM: Mesorhizobium japonicum (strain LMG 29417 / CECT 9101 / MAFF 303099) (Mesorhizobium loti (strain MAFF 303099)) SIMILARITY: Belongs to the 3-hydroxyacyl-CoA dehydrogenase family. FUNCTION: Involved in the degradation of pyridoxine (vitamin B(6)). Catalyzes the oxidation of 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate (FHMPC) by NAD(+) to 5-hydroxy-6-methylpyridine-3,4-dicarboxylate (HMPDC). Can also catalyze the reduction of FHMPC by NADH to 4-pyridoxic acid. PATHWAY: Cofactor degradation; B6 vitamer degradation. KEYWORDS: 3D-structure;Direct protein sequencing;NAD;Nucleotide-binding;Oxidoreductase " Q988D4,"PROTEIN NAMES: 2-(acetamidomethylene)succinate hydrolase (alpha-(N-acetylaminomethylene)succinic acid amidohydrolase) (AAMS amidohydrolase) PROTEIN FAMILY: AB hydrolase superfamily ORGANISM: Mesorhizobium japonicum (strain LMG 29417 / CECT 9101 / MAFF 303099) (Mesorhizobium loti (strain MAFF 303099)) SIMILARITY: Belongs to the AB hydrolase superfamily. FUNCTION: Catalyzes the final reaction in the degradation of vitamin B6 from (E)-2-(acetamidomethylene)succinate (E-2AMS) to produce succinic semialdehyde, acetate, ammonia and carbon dioxide. PATHWAY: Cofactor degradation; B6 vitamer degradation. KEYWORDS: 3D-structure;Chloride;Direct protein sequencing;Hydrolase " Q98FW0,PROTEIN NAMES: L-ribulose 3-epimerase (L-RE) (D-tagatose 3-epimerase) (DTE) (Ketose 3-epimerase) PROTEIN FAMILY: Hyi family ORGANISM: Mesorhizobium japonicum (strain LMG 29417 / CECT 9101 / MAFF 303099) (Mesorhizobium loti (strain MAFF 303099)) SIMILARITY: Belongs to the hyi family. FUNCTION: Catalyzes the epimerization of various ketoses at the C(3) position. It is able to interconvert L-ribulose with high efficiency. The enzyme can also accept other ketopentoses such as D-psicose and D-tagatose with lower efficiency. KEYWORDS: Isomerase;Manganese;Metal-binding Q99034,"PROTEIN NAMES: Acetylxylan esterase PROTEIN FAMILY: Cutinase family, Acetylxylan esterase subfamily ORGANISM: Hypocrea jecorina (Trichoderma reesei) SIMILARITY: Belongs to the cutinase family. Acetylxylan esterase subfamily. FUNCTION: Degrades acetylated xylans by cleaving acetyl side groups from the hetero-xylan backbone. PATHWAY: Glycan degradation; xylan degradation. KEYWORDS: 3D-structure;Carbohydrate metabolism;Cellulose degradation;Cleavage on pair of basic residues;Direct protein sequencing;Disulfide bond;Glycoprotein;Hydrolase;Polysaccharide degradation;Pyrrolidone carboxylic acid;Secreted;Serine esterase;Signal SUBCELLULAR LOCATION: Secreted. " Q99190,"PROTEIN NAMES: Very-long-chain enoyl-CoA reductase (Enoyl reductase TSC13) (Temperature-sensitive CSG2 suppressor protein 13) (Trans-2-enoyl-CoA reductase) PROTEIN FAMILY: Steroid 5-alpha reductase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the steroid 5-alpha reductase family. FUNCTION: Catalyzes the last of the four reactions of the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process, allows the addition of 2 carbons to the chain of long- and very long-chain fatty acids/VLCFAs per cycle. This enzyme reduces the trans-2,3-enoyl-CoA fatty acid intermediate to an acyl-CoA that can be further elongated by entering a new cycle of elongation. Thereby, it participates in the production of VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators. VLCFAs serve for instance as precursors for ceramide and sphingolipids. Required for normal biogenesis of piecemeal microautophagy of the nucleus (PMN) bleps and vesicles during nutrient stress. PATHWAY: Lipid metabolism; fatty acid biosynthesis. KEYWORDS: Endoplasmic reticulum;Fatty acid biosynthesis;Fatty acid metabolism;Lipid biosynthesis;Lipid metabolism;Membrane;NADP;Oxidoreductase;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein Note=Accumulates at nucleus-vacuole (NV) junctions. Sequestred to NV junctions by NVJ1. Accumulates in nuclear PMN bleps and vesicles during stationary phase and nitrogen starvation. MISCELLANEOUS: Present with 23600 molecules/cell in log phase SD medium." Q99210,"PROTEIN NAMES: dTTP/UTP pyrophosphatase (dTTPase/UTPase) (Maf-like protein YOR111W) (Nucleoside triphosphate pyrophosphatase) (Nucleotide pyrophosphatase) (Nucleotide PPase) PROTEIN FAMILY: Maf family, YhdE subfamily ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the Maf family. YhdE subfamily. FUNCTION: Nucleoside triphosphate pyrophosphatase that hydrolyzes dTTP and UTP. Can also hydrolyze the modified nucleotides 5-methyl-UTP (m(5)UTP) and pseudo-UTP. Has weak activity with CTP. May have a dual role in cell division arrest and in preventing the incorporation of modified nucleotides into cellular nucleic acids. KEYWORDS: Cytoplasm;Hydrolase;Nucleotide metabolism;Reference proteome SUBCELLULAR LOCATION: Cytoplasm MISCELLANEOUS: Present with 1940 molecules/cell in log phase SD medium." Q99222,"PROTEIN NAMES: ARF3-interacting protein 1 PROTEIN FAMILY: AFI1/mesA family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the AFI1/mesA family. FUNCTION: Involved in actin patch polarization. Required for maintaining a proper budding pattern in yeast cells. Required for proper polarized localization of the ADP-ribosylation factor ARF3 at the plasma membrane. KEYWORDS: Coiled coil;Cytoplasm;Reference proteome SUBCELLULAR LOCATION: Cytoplasm, perinuclear region. Cytoplasm, cell cortex. Note=Enriched at the nuclear envelope and at the plasma membrane, especially in daughter cells and at the bud neck. MISCELLANEOUS: Present with 768 molecules/cell in log phase SD medium." Q99257,PROTEIN NAMES: mRNA export factor MEX67 PROTEIN FAMILY: NXF family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the NXF family. FUNCTION: Involved in the export of mRNA from the nucleus to the cytoplasm. KEYWORDS: 3D-structure;Acetylation;Cytoplasm;Leucine-rich repeat;mRNA transport;Nucleus;Reference proteome;Repeat;Transport SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=Localizes at both the nuclear and cytoplasmic site of the pores. Shuttles between the nucleus and the cytoplasm. MISCELLANEOUS: Present with 2830 molecules/cell in log phase SD medium. Q99312,PROTEIN NAMES: IMP-specific 5'-nucleotidase 1 PROTEIN FAMILY: ISN1 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the ISN1 family. FUNCTION: IMP-specific 5'-nucleotidase involved in IMP (inosine 5'-phosphate) degradation. KEYWORDS: ATP-binding;Hydrolase;Magnesium;Metal-binding;Nucleotide metabolism;Nucleotide-binding;Reference proteome MISCELLANEOUS: Present with 1590 molecules/cell in log phase SD medium. Q99325,"PROTEIN NAMES: Autophagy-related protein 40 ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Acts as a receptor for reticulophagy. Directs autophagic sequestration of folded tubules/sheets derived from the cortical endoplasmic reticulum (cER) and the cytoplasmic endoplasmic reticulum (cytoER) into autophagosomes. Is not required for the cytoplasm-to-vacuole targeting pathway, mitophagy, pexophagy, and non-selective autophagy. KEYWORDS: 3D-structure;Autophagy;Endoplasmic reticulum;Membrane;Reference proteome;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Single-pass membrane protein Preautophagosomal structure membrane ; Single-pass membrane protein MISCELLANEOUS: Present with 1890 molecules/cell in log phase SD medium." Q99394,"PROTEIN NAMES: Trafficking protein particle complex subunit 33 (TRAPP subunit 33) (Transport protein particle 33 kDa subunit) PROTEIN FAMILY: TRAPP small subunits family, BET3 subfamily ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the TRAPP small subunits family. BET3 subfamily. FUNCTION: Component of the TRAPP I, TRAPP II and TRAPP III complexes which act as guanine nucleotide exchange factors (GEF) for YPT1. TRAPP I plays a key role in the late stages of endoplasmic reticulum to Golgi traffic. TRAPP II plays a role in intra-Golgi transport. TRAPP III plays a role in autophagosome formation. Required for sporulation. Has a role late in meiosis following DNA replication. KEYWORDS: 3D-structure;Acetylation;Autophagy;Endoplasmic reticulum;ER-Golgi transport;Golgi apparatus;Reference proteome;Transport SUBCELLULAR LOCATION: Golgi apparatus, cis-Golgi network. Endoplasmic reticulum. Preautophagosomal structure. " Q99501,"PROTEIN NAMES: GAS2-like protein 1 (GAS2-related protein on chromosome 22) (Growth arrest-specific protein 2-like 1) PROTEIN FAMILY: GAS2 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the GAS2 family. FUNCTION: Involved in the cross-linking of microtubules and microfilaments. Regulates microtubule dynamics and stability by interacting with microtubule plus-end tracking proteins, such as MAPRE1, to regulate microtubule growth along actin stress fibers. KEYWORDS: Acetylation;Alternative splicing;Cytoplasm;Cytoskeleton;Methylation;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton Cytoplasm, cytoskeleton, stress fiber Note=Colocalizes with the tips of microtubule plus ends. " Q99607,"PROTEIN NAMES: ETS-related transcription factor Elf-4 (E74-like factor 4) (Myeloid Elf-1-like factor) PROTEIN FAMILY: ETS family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the ETS family. FUNCTION: Transcriptional activator that binds to DNA sequences containing the consensus 5'-WGGA-3'. Transactivates promoters of the hematopoietic growth factor genes CSF2, IL3, IL8, and of the bovine lysozyme gene. Acts synergistically with RUNX1 to transactivate the IL3 promoter (By similarity). Transactivates the PRF1 promoter in natural killer (NK) cells and CD8+ T cells. Plays a role in the development and function of NK and NK T-cells and in innate immunity. Controls the proliferation and homing of CD8+ T-cells via the Kruppel-like factors KLF4 and KLF2 (By similarity). Controls cell senescence in a p53-dependent manner. Can also promote cellular transformation through inhibition of the p16 pathway. Is a transcriptional regulator of inflammation, controlling T-helper 17 (Th17) cells and macrophage inflammatory responses. Required for sustained transcription of anti-inflammatory genes, including IL1RN. Is a negative regulator of pro-inflammatory cytokines expression including IL17A, IL1B, IL6, TNFA and CXCL1. Down-regulates expression of TREM1, a cell surface receptor involved in the amplification of inflammatory responses (By similarity). KEYWORDS: Activator;Chromosomal rearrangement;Disease variant;DNA-binding;Nucleus;Phosphoprotein;Proto-oncogene;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus, PML body Note=Accumulation into PML nuclear bodies is mediated by PML. " Q99935,"PROTEIN NAMES: Opiorphin prepropeptide (Basic proline-rich lacrimal protein) (Proline-rich protein 1) (PRL1) [Cleaved into: Opiorphin] PROTEIN FAMILY: PROL1/PROL3 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the PROL1/PROL3 family. FUNCTION: Opiorphin is an endogenous inhibitor of neprilysin and aminopeptidase N. Inhibits the breakdown of substance P, Mca-BK2 and Met-enkephalin by neprilysin in vitro with IC(50) values of 29 uM, 33 uM and 33 uM respectively. Inhibits the breakdown of Ala-pNA by aminopeptidase N in vitro with an IC(50) of 65 uM. Has a potent analgesic effect when administered to rats by intravenous injection. KEYWORDS: Direct protein sequencing;Glycoprotein;Protease inhibitor;Pyrrolidone carboxylic acid;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted " Q99952,"PROTEIN NAMES: Tyrosine-protein phosphatase non-receptor type 18 (Brain-derived phosphatase) PROTEIN FAMILY: Protein-tyrosine phosphatase family, Non-receptor class 4 subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the protein-tyrosine phosphatase family. Non-receptor class 4 subfamily. FUNCTION: Differentially dephosphorylate autophosphorylated tyrosine kinases which are known to be overexpressed in tumor tissues. KEYWORDS: 3D-structure;Alternative splicing;Cytoplasm;Hydrolase;Nucleus;Phosphoprotein;Protein phosphatase;Reference proteome SUBCELLULAR LOCATION: Nucleus Cytoplasm " Q99958,PROTEIN NAMES: Forkhead box protein C2 (Forkhead-related protein FKHL14) (Mesenchyme fork head protein 1) (MFH-1 protein) (Transcription factor FKH-14) ORGANISM: Homo sapiens (Human) FUNCTION: Transcriptional activator. KEYWORDS: 3D-structure;Activator;Developmental protein;Disease variant;DNA-binding;Isopeptide bond;Methylation;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation;Ubl conjugation SUBCELLULAR LOCATION: Nucleus Q99J93,"PROTEIN NAMES: Interferon-induced transmembrane protein 2 (Dispanin subfamily A member 2c) (DSPA2c) (Fragilis protein 3) PROTEIN FAMILY: CD225/Dispanin family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the CD225/Dispanin family. FUNCTION: IFN-induced antiviral protein which inhibits the entry of viruses to the host cell cytoplasm, permitting endocytosis, but preventing subsequent viral fusion and release of viral contents into the cytosol. Active against multiple viruses, including influenza A virus, SARS coronavirus (SARS-CoV), Marburg virus (MARV) and Ebola virus (EBOV), Dengue virus (DNV) and West Nile virus (WNV). Can inhibit: influenza virus hemagglutinin protein-mediated viral entry, MARV and EBOV GP1,2-mediated viral entry and SARS-CoV S protein-mediated viral entry. Induces cell cycle arrest and mediates apoptosis by caspase activation and in p53-independent manner. KEYWORDS: Acetylation;Antiviral defense;Cell membrane;Endosome;Immunity;Innate immunity;Lipoprotein;Lysosome;Membrane;Palmitate;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type II membrane protein Lysosome membrane ; Single-pass type II membrane protein Late endosome membrane ; Single-pass type II membrane protein " Q99JA4,"PROTEIN NAMES: Cysteinyl leukotriene receptor 1 (CysLTR1) (Cysteinyl leukotriene D4 receptor) (LTD4 receptor) PROTEIN FAMILY: G-protein coupled receptor 1 family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the G-protein coupled receptor 1 family. FUNCTION: Receptor for cysteinyl leukotrienes mediating constriction of the microvascular smooth muscle during an inflammatory response. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. The rank order of affinities for the leukotrienes is LTD4 >> LTE4 = LTC4 >> LTB4. KEYWORDS: Alternative splicing;Cell membrane;Disulfide bond;G-protein coupled receptor;Glycoprotein;Membrane;Receptor;Reference proteome;Transducer;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. MISCELLANEOUS: MK-571, a selective antagonist, was shown to inhibit eosinophilia, bronchial hyperreactivity and microvascular leakage. Zafirlukast (Accolate) and pranlukast (Onon) were also shown to be selective antagonists." Q99K30,"PROTEIN NAMES: Epidermal growth factor receptor kinase substrate 8-like protein 2 (EPS8-like protein 2) (Epidermal growth factor receptor pathway substrate 8-related protein 2) (EPS8-related protein 2) PROTEIN FAMILY: EPS8 family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the EPS8 family. FUNCTION: Stimulates guanine exchange activity of SOS1. May play a role in membrane ruffling and remodeling of the actin cytoskeleton (By similarity). In the cochlea, is required for stereocilia maintenance in adult hair cells. KEYWORDS: Alternative splicing;Cell projection;Cytoplasm;Phosphoprotein;Reference proteome;SH3 domain SUBCELLULAR LOCATION: Cytoplasm Cell projection, stereocilium Note=Localizes at the tips of the stereocilia of the inner and outer hair cells. " Q99K82,"PROTEIN NAMES: Spermine oxidase (Polyamine oxidase 1) (PAO-1) (PAOh1) PROTEIN FAMILY: Flavin monoamine oxidase family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the flavin monoamine oxidase family. FUNCTION: Flavoenzyme which catalyzes the oxidation of spermine to spermidine. Can also use N(1)-acetylspermine and spermidine as substrates, with different affinity depending on the isoform (isozyme) and on the experimental conditions. Plays an important role in the regulation of polyamine intracellular concentration and has the potential to act as a determinant of cellular sensitivity to the antitumor polyamine analogs. May contribute to beta-alanine production via aldehyde dehydrogenase conversion of 3-amino-propanal. PATHWAY: Amine and polyamine degradation; spermine degradation. KEYWORDS: Alternative splicing;Cytoplasm;FAD;Flavoprotein;Nucleus;Oxidoreductase;Reference proteome SUBCELLULAR LOCATION: Cytoplasm.; SUBCELLULAR LOCATION: [Isoform 2]: Cytoplasm. Nucleus. MISCELLANEOUS: [Isoform 1]: Major isoform.; MISCELLANEOUS: [Isoform 2]: Active. Nuclear and cytoplasmic.; MISCELLANEOUS: [Isoform 7]: No detectable activity. Cytoplasmic.; MISCELLANEOUS: [Isoform 10]: No detectable activity. Cytoplasmic." Q99LY2,"PROTEIN NAMES: Lysophospholipase D GDPD3 (Glycerophosphodiester phosphodiesterase 7) (Glycerophosphodiester phosphodiesterase domain-containing protein 3) PROTEIN FAMILY: Glycerophosphoryl diester phosphodiesterase family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the glycerophosphoryl diester phosphodiesterase family. FUNCTION: Hydrolyzes lysoglycerophospholipids to produce lysophosphatidic acid (LPA) and the corresponding amines. Shows a preference for 1-O-alkyl-sn-glycero-3-phosphocholine (lyso-PAF), lysophosphatidylcholine (lyso-PC) and N-acylethanolamine lysophospholipids. Does not display glycerophosphodiester phosphodiesterase activity, since it cannot hydrolyze either glycerophosphoinositol or glycerophosphocholine. KEYWORDS: Cytoplasm;Endoplasmic reticulum;Hydrolase;Lipid metabolism;Membrane;Metal-binding;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Multi-pass membrane protein Cytoplasm, perinuclear region Endoplasmic reticulum membrane ; Multi-pass membrane protein Note=Partially colocalized with CANX. " Q99M15,PROTEIN NAMES: Proline-serine-threonine phosphatase-interacting protein 2 (PEST phosphatase-interacting protein 2) (Macrophage actin-associated tyrosine-phosphorylated protein) (pp37) ORGANISM: Mus musculus (Mouse) FUNCTION: Binds to F-actin. May be involved in regulation of the actin cytoskeleton. KEYWORDS: Coiled coil;Cytoplasm;Direct protein sequencing;Membrane;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cytoplasm. Membrane; Peripheral membrane protein. Q99M75,"PROTEIN NAMES: Reticulon-4 receptor (Nogo receptor) (NgR) (Nogo-66 receptor) PROTEIN FAMILY: Nogo receptor family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the Nogo receptor family. FUNCTION: Receptor for RTN4, OMG and MAG. Functions as a receptor for the sialylated gangliosides GT1b and GM1. Besides, functions as a receptor for chondroitin sulfate proteoglycans. Can also bind heparin. Intracellular signaling cascades are triggered via the coreceptor NGFR (By similarity). Signaling mediates activation of Rho and downstream reorganization of the actin cytoskeleton. Mediates axonal growth inhibition. May play a role in regulating axon regeneration and neuronal plasticity in the adult central nervous system. Plays a role in postnatal brain development. Required for normal axon migration across the brain midline and normal formation of the corpus callosum (By similarity). Protects motoneurons against apoptosis; protection against apoptosis is probably mediated via interaction with MAG. Acts in conjunction with RTN4 and LINGO1 in regulating neuronal precursor cell motility during cortical development. Like other family members, plays a role in restricting the number dendritic spines and the number of synapses that are formed during brain development. KEYWORDS: 3D-structure;Cell membrane;Cell projection;Disulfide bond;Glycoprotein;GPI-anchor;Leucine-rich repeat;Lipoprotein;Membrane;Receptor;Reference proteome;Repeat;Signal SUBCELLULAR LOCATION: Cell membrane ; Lipid-anchor, GPI-anchor Membrane raft Cell projection, dendrite Perikaryon Cell projection, axon Note=Detected along dendrites and axons, close to synapses, but clearly excluded from synapses. " Q99MS8,"PROTEIN NAMES: Tubulin polyglutamylase complex subunit 1 (PGs1) (p32) ORGANISM: Mus musculus (Mouse) FUNCTION: Subunit of the tubulin polyglutamylase complex (TPGC). The complex mediates cilia and flagella polyglutamylation which is essential for their biogenesis and motility. May act in the targeting of the tubulin polyglutamylase complex. Required for the development of the spermatid flagellum. KEYWORDS: Cell projection;Cilium;Cytoplasm;Cytoskeleton;Developmental protein;Differentiation;Flagellum;Microtubule;Phosphoprotein;Reference proteome;Spermatogenesis SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, cilium axoneme Cytoplasm, cytoskeleton, flagellum axoneme Cytoplasm, cytoskeleton, cilium basal body Cytoplasm, cytoskeleton, flagellum basal body Cell projection, axon Cell projection, dendrite Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Note=Associated with microtubules from neurites, centrosomes, basal bodies and axonemes. " Q99MT2,PROTEIN NAMES: MutS protein homolog 4 (mMsh4) PROTEIN FAMILY: DNA mismatch repair MutS family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the DNA mismatch repair MutS family. FUNCTION: Involved in meiotic recombination. Required for reciprocal recombination and proper segregation of homologous chromosomes at meiosis. KEYWORDS: ATP-binding;Chromosome;DNA-binding;Meiosis;Nucleotide-binding;Reference proteome SUBCELLULAR LOCATION: Chromosome Q99N34,PROTEIN NAMES: DNA fragmentation factor subunit beta (Caspase-activated deoxyribonuclease) (CAD) (Caspase-activated DNase) (DNA fragmentation factor 40 kDa subunit) (DFF-40) ORGANISM: Rattus norvegicus (Rat) FUNCTION: Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology. KEYWORDS: Apoptosis;Cytoplasm;Hydrolase;Nuclease;Nucleus;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Nucleus Q99NA2,"PROTEIN NAMES: Transcription factor Atoh8 (Helix-loop-helix protein mATH-6) (mATH6) (Okadin) (Protein atonal homolog 8) ORGANISM: Mus musculus (Mouse) FUNCTION: Transcription factor that binds a palindromic (canonical) core consensus DNA sequence 5'-CANNTG- 3' known as an E-box element, possibly as a heterodimer with other bHLH proteins (By similarity). Regulates endothelial cell proliferation, migration and tube-like structures formation (By similarity). Modulates endothelial cell differentiation through NOS3 (By similarity). May be implicated in specification and differentiation of neuronal cell lineages in the brain. May participate in kidney development and may be involved in podocyte differentiation. During early embryonic development is involved in tissue-specific differentiation processes that are dependent on class II bHLH factors and namely modulates the differentiation program initiated by the pro-endocrine factor NEUROG3. During myogenesis, may play a role during the transition of myoblasts from the proliferative phase to the differentiation phase. Positively regulates HAMP transcription in two ways, firstly by acting directly on the HAMP promoter via E-boxes binding and indirectly through increased phosphorylation of SMAD protein complex (By similarity). Repress NEUROG3-dependent gene activation in a gene-specific manner through at least two mechanisms; requires only either the sequestering of a general partner such as TCF3 through heterodimerization, either also requires binding of the bHLH domain to DNA via a basic motif. KEYWORDS: Cytoplasm;Developmental protein;Differentiation;DNA-binding;Neurogenesis;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus Nucleus speckle Cytoplasm " Q99NB7,"PROTEIN NAMES: Acetyl-coenzyme A thioesterase (Acyl-CoA thioester hydrolase 12) (Acyl-coenzyme A thioesterase 12) (Acyl-CoA thioesterase 12) (Cytoplasmic acetyl-CoA hydrolase 1) (CACH-1) (rACH) (rCACH-1) ORGANISM: Rattus norvegicus (Rat) FUNCTION: Catalyzes the hydrolysis of acyl-CoAs into free fatty acids and coenzyme A (CoASH), regulating their respective intracellular levels. Preferentially hydrolyzes acetyl-CoA. PATHWAY: Lipid metabolism; fatty acid metabolism. KEYWORDS: Allosteric enzyme;Cytoplasm;Direct protein sequencing;Fatty acid metabolism;Hydrolase;Lipid metabolism;Reference proteome;Repeat;Serine esterase SUBCELLULAR LOCATION: Cytoplasm, cytosol " Q99P91,PROTEIN NAMES: Transmembrane glycoprotein NMB (DC-HIL) (Dendritic cell-associated transmembrane protein) (Osteoactivin) PROTEIN FAMILY: PMEL/NMB family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the PMEL/NMB family. FUNCTION: Could be a melanogenic enzyme. KEYWORDS: Cell membrane;Endosome;Glycoprotein;Membrane;Phosphoprotein;Reference proteome;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein Melanosome membrane; Single-pass type I membrane protein Early endosome membrane; Single-pass type I membrane protein Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Q99PE7,"PROTEIN NAMES: ATP-binding cassette sub-family G member 5 (Sterolin-1) PROTEIN FAMILY: ABC transporter superfamily, ABCG family, Eye pigment precursor importer subfamily ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily. FUNCTION: ABCG5 and ABCG8 form an obligate heterodimer that mediates Mg(2+)- and ATP-dependent sterol transport across the cell membrane. Plays an essential role in the selective transport of dietary plant sterols and cholesterol in and out of the enterocytes and in the selective sterol excretion by the liver into bile. Required for normal sterol homeostasis. The heterodimer with ABCG8 has ATPase activity. KEYWORDS: ATP-binding;Cell membrane;Glycoprotein;Lipid transport;Magnesium;Membrane;Metal-binding;Nucleotide-binding;Reference proteome;Translocase;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Apical cell membrane ; Multi-pass membrane protein " Q99PJ2,"PROTEIN NAMES: E3 ubiquitin-protein ligase TRIM8 (Glioblastoma-expressed RING finger protein) (RING finger protein 27) (RING-type E3 ubiquitin transferase TRIM8) (Tripartite motif-containing protein 8) PROTEIN FAMILY: TRIM/RBCC family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the TRIM/RBCC family. FUNCTION: E3 ubiquitin-protein ligase that participates in multiple biological processes including cell survival, differentiation, apoptosis, and in particular, the innate immune response. Participates in the activation of interferon-gamma signaling by promoting proteasomal degradation of the repressor SOCS1. Plays a positive role in the TNFalpha and IL-1beta signaling pathways. Mechanistically, induces the 'Lys-63'-linked polyubiquitination of MAP3K7/TAK1 component leading to the activation of NF-kappa-B (By similarity). Modulates also STAT3 activity through negative regulation of PIAS3, either by degradation of PIAS3 through the ubiquitin-proteasome pathway or exclusion of PIAS3 from the nucleus (By similarity). Negatively regulates TLR3/4-mediated innate immune response by catalyzing 'Lys-6'- and 'Lys-33'-linked polyubiquitination of TICAM1 and thereby disrupting the TICAM1-TBK1 interaction. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Coiled coil;Immunity;Innate immunity;Metal-binding;Reference proteome;Repeat;Transferase;Ubl conjugation pathway;Zinc;Zinc-finger " Q99PM3,PROTEIN NAMES: Transcription initiation factor IIA subunit 1 (General transcription factor IIA subunit 1) [Cleaved into: Transcription initiation factor IIA alpha chain (TFIIA p35 subunit); Transcription initiation factor IIA beta chain (TFIIA p19 subunit)] PROTEIN FAMILY: TFIIA subunit 1 family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the TFIIA subunit 1 family. FUNCTION: TFIIA is a component of the transcription machinery of RNA polymerase II and plays an important role in transcriptional activation. TFIIA in a complex with TBP mediates transcriptional activity (By similarity). KEYWORDS: Acetylation;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus Q99PT3,"PROTEIN NAMES: INO80 complex subunit B (High mobility group AT-hook 1-like 4) (PAP-1-associated protein 1) (PAPA-1) (Zinc finger HIT domain-containing protein 4) ORGANISM: Mus musculus (Mouse) FUNCTION: Proposed core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair. KEYWORDS: Coiled coil;DNA damage;DNA recombination;DNA repair;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Nucleus, nucleolus " Q9A1S2,"PROTEIN NAMES: Pilin (Lancefield T antigen) (Pilus backbone structural protein) PROTEIN FAMILY: Streptococcus pilin family ORGANISM: Streptococcus pyogenes serotype M1 SIMILARITY: Belongs to the Streptococcus pilin family. FUNCTION: Major component of the pilus. A stack of the pilin subunits, joined by intermolecular isopeptide bonds, forms the pilus. The pilus is required for bacterial adhesion to host cells, for bacterial aggregation, and for biofilm formation. KEYWORDS: 3D-structure;Cell wall;Fimbrium;Isopeptide bond;Peptidoglycan-anchor;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted, cell wall; Peptidoglycan-anchor. Fimbrium. Note=Attached to the cell wall by a peptidoglycan anchor. " Q9AI65,"PROTEIN NAMES: Alpha-glucosidase PROTEIN FAMILY: Glycosyl hydrolase 4 family ORGANISM: Erwinia rhapontici (Pectobacterium rhapontici) SIMILARITY: Belongs to the glycosyl hydrolase 4 family. FUNCTION: Alpha-glucosidase with broad specificity. Hydrolyzes maltose, palatinose, maltulose, trehalose, trehalulose, turanose, leucrose, sucrose and maltitol. Is not active against alpha-galactosides, e.g. melibiose, and alpha-mannosides. Shows an obligate requirement for an O-alpha-glycosidic linkage, since it is not able to cleave beta-glycosidic bonds (cellobiose, gentiobiose, lactose, sophorose or laminaribiose). Cannot hydrolyze phosphorylated alpha-glucosides derivatives. Seems to be involved in the degradation of palatinose, a sucrose isomer that is formed as a reserve material under conditions of excess carbon availability, sequestered in a form unavailable to competitors such as fungi or the host plant, and whose consumption appears to be postponed until the preferentially metabolized carbon source (e.g. sucrose) is depleted. PATHWAY: Glycan degradation; palatinose degradation. KEYWORDS: Calcium;Carbohydrate metabolism;Cobalt;Direct protein sequencing;Glycosidase;Hydrolase;Iron;Magnesium;Manganese;Metal-binding;NAD;Nickel " Q9ASS2,"PROTEIN NAMES: Protein FREE1 (FYVE domain protein required for endosomal sorting 1) (FYVE domain-containing protein 1) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Endosomal sorting complex required for transport (ESCRT) component regulating multivesicular body (MVB) protein sorting and plant growth. Required for the formation of intra-luminal vesicles (ILVs)in MVBs. Binds to phosphatidylinositol-3-phosphate (PI3P) and ubiquitin. Controls IRT1 recycling to the plasma membrane and impacts the polar delivery of this transporter to the outer plasma membrane domain. Regulates ubiquitin-dependent membrane protein degradation, vacuolar transport, autophagy, and vacuole biogenesis. ESCRT component that binds ubiquitin and regulates vacuolar sorting of proteins. Attenuates abscisic acid (ABA) signaling through RSL1-triggered degradation of the ABA receptors PYR1 and PYL4. Interacts with PYL4 and PYR1, and delivers the ubiquitinated ABA receptors as cargo to the vacuolar degradation pathway. In response to ABA, is phosphorylated by SnRK2 kinases which mediate FREE1 nuclear import. In the nucleus, interacts with the ABA-responsive transcription factors ABF4 and ABI5 to reduce their ability to bind to their cis-regulatory sequences of downstream genes, thus leading to transcriptional inhibition of ABA signaling pathway. Negatively regulates salt stress tolerance via a negative feedback loop involving ABA signaling pathway. KEYWORDS: Abscisic acid signaling pathway;Coiled coil;Cytoplasm;Endosome;Membrane;Metal-binding;Nucleus;Phosphoprotein;Protein transport;Reference proteome;Transport;Ubl conjugation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cytoplasm Prevacuolar compartment membrane ; Peripheral membrane protein Late endosome Endosome, multivesicular body Nucleus Note=In response to abscisic acid (ABA), SnRK2 kinases phosphorylate FREE1, a step requisite for ABA-induced FREE1 nuclear import. MISCELLANEOUS: Reduction-of-function FREE1 alleles exhibit enhanced sensitivity to abscisic acid-mediated inhibition of seedling establishment. Seedlings overexpressing FREE1 exhibit increased sensitivity to salt stress." Q9BKJ9,PROTEIN NAMES: Lysosome membrane protein 2-B (Lysosome membrane protein II-2) (LIMP II-2) PROTEIN FAMILY: CD36 family ORGANISM: Dictyostelium discoideum (Social amoeba) SIMILARITY: Belongs to the CD36 family. FUNCTION: May act as a lysosomal receptor (By similarity). May be involved in macropinocytosis and fluid phase exocytosis. KEYWORDS: Glycoprotein;Lysosome;Membrane;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Lysosome membrane ; Multi-pass membrane protein Note=Localizes to membranes of endolysosomal vesicles and macropinosomes. Q9BQA5,PROTEIN NAMES: Histone H4 transcription factor (Histone nuclear factor P) (HiNF-P) (MBD2-interacting zinc finger protein) (Methyl-CpG-binding protein 2-interacting zinc finger protein) ORGANISM: Homo sapiens (Human) FUNCTION: Transcriptional repressor that binds to the consensus sequence 5'-CGGACGTT-3' and to the RB1 promoter. Transcriptional activator that promotes histone H4 gene transcription at the G1/S phase transition in conjunction with NPAT. Also activates transcription of the ATM and PRKDC genes. Autoregulates its expression by associating with its own promoter. KEYWORDS: Activator;Alternative splicing;DNA-binding;Metal-binding;Nucleus;Reference proteome;Repeat;Repressor;Transcription;Transcription regulation;Ubl conjugation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Note=Associated with discrete nuclear foci. Q9BRA0,"PROTEIN NAMES: N-alpha-acetyltransferase 38, NatC auxiliary subunit (LSM domain-containing protein 1) (Phosphonoformate immuno-associated protein 2) PROTEIN FAMILY: SnRNP Sm proteins family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the snRNP Sm proteins family. FUNCTION: Auxillary component of the N-terminal acetyltransferase C (NatC) complex which catalyzes acetylation of N-terminal methionine residues. KEYWORDS: 3D-structure;Acetylation;Alternative splicing;Coiled coil;Cytoplasm;Nucleus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Nucleus " Q9BRQ0,PROTEIN NAMES: Pygopus homolog 2 ORGANISM: Homo sapiens (Human) FUNCTION: Involved in signal transduction through the Wnt pathway. KEYWORDS: 3D-structure;Acetylation;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Wnt signaling pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Q9BRS2,"PROTEIN NAMES: Serine/threonine-protein kinase RIO1 (RIO kinase 1) PROTEIN FAMILY: Protein kinase superfamily, RIO-type Ser/Thr kinase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the protein kinase superfamily. RIO-type Ser/Thr kinase family. FUNCTION: Involved in the final steps of cytoplasmic maturation of the 40S ribosomal subunit. Involved in processing of 18S-E pre-rRNA to the mature 18S rRNA. Required for the recycling of NOB1 and PNO1 from the late 40S precursor. The association with the very late 40S subunit intermediate may involve a translation-like checkpoint point cycle preceeding the binding to the 60S ribosomal subunit (By similarity). Despite the protein kinase domain is proposed to act predominantly as an ATPase (By similarity). The catalytic activity regulates its dynamic association with the 40S subunit (By similarity). In addition to its role in ribosomal biogenesis acts as an adapter protein by recruiting NCL/nucleolin the to PRMT5 complex for its symmetrical methylation. KEYWORDS: 3D-structure;ATP-binding;Cytoplasm;Direct protein sequencing;Hydrolase;Kinase;Magnesium;Metal-binding;Nucleotide-binding;Phosphoprotein;Reference proteome;Ribosome biogenesis;Serine/threonine-protein kinase;Transferase SUBCELLULAR LOCATION: Cytoplasm, cytosol " Q9BT88,"PROTEIN NAMES: Synaptotagmin-11 (Synaptotagmin XI) (SytXI) PROTEIN FAMILY: Synaptotagmin family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the synaptotagmin family. FUNCTION: Synaptotagmin family member involved in vesicular and membrane trafficking which does not bind Ca(2+). Inhibits clathrin-mediated and bulk endocytosis, functions to ensure precision in vesicle retrieval. Plays an important role in dopamine transmission by regulating endocytosis and the vesicle-recycling process. Essential component of a neuronal vesicular trafficking pathway that differs from the synaptic vesicle trafficking pathway but is crucial for development and synaptic plasticity. In macrophages and microglia, inhibits the conventional cytokine secretion, of at least IL6 and TNF, and phagocytosis. In astrocytes, regulates lysosome exocytosis, mechanism required for the repair of injured astrocyte cell membrane (By similarity). Required for the ATP13A2-mediated regulation of the autophagy-lysosome pathway. KEYWORDS: Calcium;Cell projection;Cytoplasmic vesicle;Endosome;Golgi apparatus;Lysosome;Membrane;Metal-binding;Phosphoprotein;Reference proteome;Repeat;Synapse;Transmembrane;Transmembrane helix;Ubl conjugation SUBCELLULAR LOCATION: Cytoplasmic vesicle membrane ; Single-pass membrane protein Perikaryon Golgi apparatus, trans-Golgi network membrane ; Single-pass membrane protein Recycling endosome membrane ; Single-pass membrane protein Lysosome membrane ; Single-pass membrane protein Cytoplasmic vesicle, phagosome Cell projection, axon Cell projection, dendrite Postsynaptic density Recycling endosome membrane ; Single-pass membrane protein Cytoplasmic vesicle, clathrin-coated vesicle membrane ; Single-pass membrane protein Perikaryon Note=Localized in vesicles that travels in axonal and dendritic shafts in both anterograde and retrograde directions. In macrophages and microglia, recruited in phagosomes at early stages of phagocytosis (By similarity). Found in the core of the Lewy bodies in the brain of sporadic Parkinson disease patients. " Q9BU64,"PROTEIN NAMES: Centromere protein O (CENP-O) (Interphase centromere complex protein 36) PROTEIN FAMILY: CENP-O/MCM21 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the CENP-O/MCM21 family. FUNCTION: Component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. May be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex. Modulates the kinetochore-bound levels of NDC80 complex. KEYWORDS: 3D-structure;Alternative splicing;Centromere;Chromosome;Coiled coil;Kinetochore;Nucleus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Nucleus. Chromosome, centromere. Chromosome, centromere, kinetochore. Note=The CENPA-CAD complex is probably recruited on centromeres by the CENPA-NAC complex. " Q9BUB7,"PROTEIN NAMES: Transmembrane protein 70, mitochondrial PROTEIN FAMILY: TMEM70 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the TMEM70 family. FUNCTION: Scaffold protein that participates in the c-ring assembly of mitochondrial ATP synthase (F(1)F(0) ATP synthase or complex V) by facilitating the membrane insertion and oligomer formation of the subunit c/ATP5MC1 through its interaction. Therefore, participates in the early stage of mitochondrial ATP synthase biogenesis and also protects subunit c/ATP5MC1 against intramitochondrial proteolysis. In addition, binds the mitochondrial proton-transporting ATP synthase complexes I and may play a role in the stability of its membrane-bound subassemblies. KEYWORDS: Alternative splicing;Membrane;Mitochondrion;Mitochondrion inner membrane;Primary mitochondrial disease;Reference proteome;Transit peptide;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Mitochondrion inner membrane ; Multi-pass membrane protein Note=Mostly located within the inner cristae membrane. " Q9BV44,PROTEIN NAMES: tRNA (guanine(6)-N2)-methyltransferase THUMP3 (THUMP domain-containing protein 3) PROTEIN FAMILY: Methyltransferase superfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the methyltransferase superfamily. FUNCTION: Methyltransferase which catalyzes the formation of N(2)-methylguanosine at position 6 in a broad range of tRNA substrates containing the characteristic 3'-CCA terminus of mature tRNAs. Also catalyzes the formation of N(2)-methylguanosine at position 7 of tRNA(Trp). Requires the methyltransferase adapter protein TRM112 for tRNA methyltransferase activity. KEYWORDS: Cytoplasm;Methyltransferase;Reference proteome;RNA-binding;S-adenosyl-L-methionine;Transferase;tRNA processing;tRNA-binding SUBCELLULAR LOCATION: Cytoplasm Q9BVG8,"PROTEIN NAMES: Kinesin-like protein KIFC3 PROTEIN FAMILY: TRAFAC class myosin-kinesin ATPase superfamily, Kinesin family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. FUNCTION: Minus-end microtubule-dependent motor protein. Involved in apically targeted transport (By similarity). Required for zonula adherens maintenance. KEYWORDS: 3D-structure;Alternative splicing;ATP-binding;Cell junction;Coiled coil;Cytoplasm;Cytoplasmic vesicle;Cytoskeleton;Membrane;Microtubule;Motor protein;Nucleotide-binding;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cell junction, adherens junction Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Cytoplasmic vesicle membrane ; Peripheral membrane protein Note=Apical cell membrane. On membrane organelles immediately beneath the apical plasma membrane of renal tubular epithelial cells. Localized in the distal tubules and loops of Henle in the kidney, but not in the proximal tubules or the glomeruli, with stronger staining in the apical area of these epithelial cells (By similarity). Localizes along zonula adherens only at mature cell-cell contacts. MISCELLANEOUS: [Isoform 3]: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay." Q9BVH7,"PROTEIN NAMES: Alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase 5 (GD1 alpha synthase) (GalNAc alpha-2,6-sialyltransferase V) (ST6GalNAc V) (ST6GalNAcV) (Sialyltransferase 7E) (SIAT7-E) PROTEIN FAMILY: Glycosyltransferase 29 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the glycosyltransferase 29 family. FUNCTION: Predominantly catalyzes the biosynthesis of ganglioside GD1alpha from GM1b in the brain, by transferring the sialyl group (N-acetyl-alpha-neuraminyl or NeuAc) from CMP-NeuAc to the GalNAc residue on the NeuAc-alpha-2,3-Gal-beta-1,3-GalNAc sequence of GM1b. GD1alpha is a critical molecule in the communication and interaction between neuronal cells and their supportive cells, particularly in brain tissues, and functions as an adhesion molecule in the process of metastasis (By similarity). Also shows activity towards sialyl Lc4Cer (N-acetyl-alpha-neuraminosyl-(2->3)-beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1')-N-acyl-sphing-4-enine) generating disialyl Lc4Cer, which can lead to the synthesis of disialyl Lewis a (Le(a)), suggested to be a cancer-associated antigen. PATHWAY: Glycolipid biosynthesis. KEYWORDS: Disulfide bond;Glycoprotein;Glycosyltransferase;Golgi apparatus;Lipid metabolism;Membrane;Reference proteome;Sialic acid;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus membrane ; Single-pass type II membrane protein " Q9BVV7,"PROTEIN NAMES: Mitochondrial import inner membrane translocase subunit Tim21 (TIM21-like protein, mitochondrial) PROTEIN FAMILY: TIM21 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the TIM21 family. FUNCTION: Participates in the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. Also required for assembly of mitochondrial respiratory chain complex I and complex IV as component of the MITRAC (mitochondrial translation regulation assembly intermediate of cytochrome c oxidase complex) complex. Probably shuttles between the presequence translocase and respiratory-chain assembly intermediates in a process that promotes incorporation of early nuclear-encoded subunits into these complexes. KEYWORDS: Membrane;Mitochondrion;Protein transport;Reference proteome;Transit peptide;Translocation;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Mitochondrion membrane ; Single-pass membrane protein " Q9BVW5,"PROTEIN NAMES: TIMELESS-interacting protein PROTEIN FAMILY: CSM3 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the CSM3 family. FUNCTION: Plays an important role in the control of DNA replication and the maintenance of replication fork stability. Important for cell survival after DNA damage or replication stress. May be specifically required for the ATR-CHEK1 pathway in the replication checkpoint induced by hydroxyurea or ultraviolet light. Forms a complex with TIMELESS and this complex regulates DNA replication processes under both normal and stress conditions, stabilizes replication forks and influences both CHEK1 phosphorylation and the intra-S phase checkpoint in response to genotoxic stress. KEYWORDS: 3D-structure;Cell cycle;Cell division;Cytoplasm;DNA damage;Mitosis;Nucleus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Nucleus " Q9BWE0,"PROTEIN NAMES: Replication initiator 1 (60 kDa origin-specific DNA-binding protein) (60 kDa replication initiation region protein) (ATT-binding protein) (DHFR oribeta-binding protein RIP60) (Zinc finger protein 464) ORGANISM: Homo sapiens (Human) FUNCTION: Sequence-specific double-stranded DNA-binding protein required for initiation of chromosomal DNA replication. Binds on 5'-ATT-3' reiterated sequences downstream of the origin of bidirectional replication (OBR) and a second, homologous ATT sequence of opposite orientation situated within the OBR zone. Facilitates DNA bending. KEYWORDS: Acetylation;Alternative splicing;Direct protein sequencing;DNA replication;DNA-binding;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Repeat;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus " Q9BWQ6,"PROTEIN NAMES: Protein YIPF2 (YIP1 family member 2) PROTEIN FAMILY: YIP1 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the YIP1 family. KEYWORDS: Acetylation;Endosome;Golgi apparatus;Membrane;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus, cis-Golgi network membrane ; Multi-pass membrane protein Golgi apparatus, trans-Golgi network membrane Late endosome membrane Note=Mainly localizes within medial-/trans-Golgi and trans-Golgi network (TGN), while less so within cis-Golgi. " Q9BWU0,PROTEIN NAMES: Kanadaptin (Human lung cancer oncogene 3 protein) (HLC-3) (Kidney anion exchanger adapter protein) (Solute carrier family 4 anion exchanger member 1 adapter protein) ORGANISM: Homo sapiens (Human) KEYWORDS: 3D-structure;Alternative initiation;Coiled coil;Cytoplasm;Isopeptide bond;Nucleus;Phosphoprotein;Reference proteome;Ubl conjugation SUBCELLULAR LOCATION: Nucleus Cytoplasm Note=Mainly nuclear. Small amounts are found in the cytoplasm. MISCELLANEOUS: Isoform 2 is a prediction based on conservation with orthologs. Q9BX70,PROTEIN NAMES: BTB/POZ domain-containing protein 2 ORGANISM: Homo sapiens (Human) KEYWORDS: 3D-structure;Alternative splicing;Cytoplasm;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Note=Localizes to punctate or elongated cytoplasmic bodies. Q9BXF6,"PROTEIN NAMES: Rab11 family-interacting protein 5 (Rab11-FIP5) (Gamma-SNAP-associated factor 1) (Gaf-1) (Phosphoprotein pp75) (Rab11-interacting protein Rip11) ORGANISM: Homo sapiens (Human) FUNCTION: Rab effector involved in protein trafficking from apical recycling endosomes to the apical plasma membrane. Involved in insulin granule exocytosis. May regulate V-ATPase intracellular transport in response to extracellular acidosis. KEYWORDS: Cytoplasm;Cytoplasmic vesicle;Endosome;Golgi apparatus;Host-virus interaction;Membrane;Mitochondrion;Phosphoprotein;Protein transport;Reference proteome;Transport SUBCELLULAR LOCATION: Cytoplasm. Recycling endosome membrane; Peripheral membrane protein. Early endosome membrane ; Peripheral membrane protein Golgi apparatus membrane ; Peripheral membrane protein Cytoplasmic vesicle, secretory vesicle membrane ; Peripheral membrane protein Mitochondrion membrane; Peripheral membrane protein. MISCELLANEOUS: Antibodies against RIP11 are found in sera from patients with autoimmune diseases such as systemic lupus erythematosus (SLE) or Sjoegren syndrome (SS). It is also found in the sera from mothers of children with neonatal lupus erythematosus (NLE)." Q9BXJ3,"PROTEIN NAMES: Complement C1q tumor necrosis factor-related protein 4 (C1q/TNF-related protein 4) ORGANISM: Homo sapiens (Human) FUNCTION: May be involved in the regulation of the inflammatory network. Its role as pro- or anti-inflammatory seems to be context dependent. Seems to have some role in regulating food intake and energy balance when administered in the brain. This effect is sustained over a two-day period, and it is accompanied by decreased expression of orexigenic neuropeptides in the hypothalamus 3 hours post-injection (By similarity). KEYWORDS: Cytokine;Direct protein sequencing;Disease variant;Reference proteome;Repeat;Secreted;Signal SUBCELLULAR LOCATION: Secreted " Q9BXK5,PROTEIN NAMES: Bcl-2-like protein 13 (Bcl2-L-13) (Bcl-rambo) (Protein Mil1) PROTEIN FAMILY: Bcl-2 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the Bcl-2 family. FUNCTION: May promote the activation of caspase-3 and apoptosis. KEYWORDS: Alternative splicing;Apoptosis;Membrane;Mitochondrion;Nucleus;Phosphoprotein;Reference proteome;Repeat;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: [Isoform 2]: Mitochondrion membrane ; Single-pass membrane protein Nucleus.; SUBCELLULAR LOCATION: [Isoform 1]: Nucleus Q9BXN1,"PROTEIN NAMES: Asporin (Periodontal ligament-associated protein 1) (PLAP-1) PROTEIN FAMILY: Small leucine-rich proteoglycan (SLRP) family, SLRP class I subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily. FUNCTION: Negatively regulates periodontal ligament (PDL) differentiation and mineralization to ensure that the PDL is not ossified and to maintain homeostasis of the tooth-supporting system. Inhibits BMP2-induced cytodifferentiation of PDL cells by preventing its binding to BMPR1B/BMP type-1B receptor, resulting in inhibition of BMP-dependent activation of SMAD proteins (By similarity). Critical regulator of TGF-beta in articular cartilage and plays an essential role in cartilage homeostasis and osteoarthritis (OA) pathogenesis. Negatively regulates chondrogenesis in the articular cartilage by blocking the TGF-beta/receptor interaction on the cell surface and inhibiting the canonical TGF-beta/Smad signal. Binds calcium and plays a role in osteoblast-driven collagen biomineralization activity. KEYWORDS: Biomineralization;Calcium;Direct protein sequencing;Disulfide bond;Extracellular matrix;Glycoprotein;Leucine-rich repeat;Reference proteome;Repeat;Secreted;Signal;Triplet repeat expansion SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix " Q9BY15,"PROTEIN NAMES: Adhesion G protein-coupled receptor E3 (EGF-like module receptor 3) (EGF-like module-containing mucin-like hormone receptor-like 3) PROTEIN FAMILY: G-protein coupled receptor 2 family, Adhesion G-protein coupled receptor (ADGR) subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily. FUNCTION: Orphan receptor that may play a role myeloid-myeloid interactions during immune and inflammatory responses. A ligand for the soluble form of this receptor is present at the surface of monocytes-derived macrophages and activated neutrophils. KEYWORDS: Alternative splicing;Calcium;Cell membrane;Disulfide bond;EGF-like domain;G-protein coupled receptor;Glycoprotein;Membrane;Receptor;Reference proteome;Repeat;Secreted;Signal;Transducer;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.; SUBCELLULAR LOCATION: [Isoform 3]: Secreted. MISCELLANEOUS: Has no murine ortholog.; MISCELLANEOUS: [Isoform 3]: Due to a 40-nucleotide deletion (nucleotides 439-479) resulting in a frameshift leading to a premature stop codon and the production of a truncated soluble form." Q9BY84,"PROTEIN NAMES: Dual specificity protein phosphatase 16 (Mitogen-activated protein kinase phosphatase 7) (MAP kinase phosphatase 7) (MKP-7) PROTEIN FAMILY: Protein-tyrosine phosphatase family, Non-receptor class dual specificity subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily. FUNCTION: Dual specificity protein phosphatase involved in the inactivation of MAP kinases. Dephosphorylates MAPK10 bound to ARRB2. KEYWORDS: 3D-structure;Acetylation;Alternative splicing;Cytoplasm;Cytoplasmic vesicle;Hydrolase;Nucleus;Phosphoprotein;Protein phosphatase;Reference proteome SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Cytoplasmic vesicle. Note=After dissociation upon AGTR stimulation, re-associates with ARRB2 on endocytic vesicles. " Q9BYN7,PROTEIN NAMES: Zinc finger protein 341 PROTEIN FAMILY: Krueppel C2H2-type zinc-finger protein family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein family. FUNCTION: Transcriptional activator of STAT3 involved in the regulation of immune homeostasis. Also able to activate STAT1 transcription. KEYWORDS: Alternative splicing;Disease variant;DNA-binding;Metal-binding;Nucleus;Reference proteome;Repeat;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Q9BYV6,"PROTEIN NAMES: Tripartite motif-containing protein 55 (Muscle-specific RING finger protein 2) (MuRF-2) (MuRF2) (RING finger protein 29) ORGANISM: Homo sapiens (Human) FUNCTION: E3 ubiquitin ligase that plays an important role in regulating cardiac development and contractility, muscle growth, metabolism, and fiber-type differentiation. Acts as a critical factor that regulates cardiomyocyte size during development in concert with TRIM63 by regulating E2F1-mediated gene expression (By similarity). Plays a role in apoptosis induction in cardiomyocytes by promoting ubiquitination of the DUSP1 phosphatase. Promotes non-canonical NF-kappa-B signaling and B-cell-mediated immune responses by mediating NFKB2 'Lys-48'-linked ubiquitination and processing. In turn, NFKB2 is further processed by valosin-containing protein/VCP, an ATPase that mediates ubiquitin-dependent protein degradation by the proteasome. May play a role in preventing macrophages from producing inflammatory factors and migrating by downregulating the level of nuclear NF-kappa-B subunit RELA. Modifies also PPARG via polyubiquitination and accelerates PPARG proteasomal degradation to inhibit its activity. KEYWORDS: Alternative splicing;Coiled coil;Cytoplasm;Metal-binding;Muscle protein;Nucleus;Reference proteome;Transferase;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Cytoplasm Note=TLR4 signaling pathway promotes nuclear translocation. " Q9BYV7,"PROTEIN NAMES: Carotenoid-cleaving dioxygenase, mitochondrial (B-diox-II) (Beta,beta-carotene 9',10'-oxygenase) (Beta-carotene dioxygenase 2) PROTEIN FAMILY: Carotenoid oxygenase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the carotenoid oxygenase family. FUNCTION: Broad specificity mitochondrial dioxygenase that mediates the asymmetric oxidative cleavage of carotenoids. Cleaves carotenes (pure hydrocarbon carotenoids) such as all-trans-beta-carotene and lycopene as well as xanthophylls (oxygenated carotenoids) such as zeaxanthin, lutein and beta-cryptoxanthin at both the 9,10 and the 9',10' carbon-carbon double bond. Through its function in carotenoids metabolism regulates oxidative stress and the production of important signaling molecules. KEYWORDS: Alternative splicing;Dioxygenase;Iron;Lipid metabolism;Metal-binding;Mitochondrion;Oxidoreductase;Reference proteome SUBCELLULAR LOCATION: Mitochondrion " Q9BYV9,"PROTEIN NAMES: Transcription regulator protein BACH2 (BTB and CNC homolog 2) PROTEIN FAMILY: BZIP family, CNC subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the bZIP family. CNC subfamily. FUNCTION: Transcriptional regulator that acts as a repressor or activator (By similarity). Binds to Maf recognition elements (MARE) (By similarity). Plays an important role in coordinating transcription activation and repression by MAFK (By similarity). Induces apoptosis in response to oxidative stress through repression of the antiapoptotic factor HMOX1. Positively regulates the nuclear import of actin (By similarity). Is a key regulator of adaptive immunity, crucial for the maintenance of regulatory T-cell function and B-cell maturation. KEYWORDS: 3D-structure;Activator;Cytoplasm;Disease variant;Disulfide bond;DNA-binding;Isopeptide bond;Nucleus;Phosphoprotein;Reference proteome;Repressor;Transcription;Transcription regulation;Ubl conjugation SUBCELLULAR LOCATION: Cytoplasm Nucleus Note=Nucleocytoplasmic shuttling is controlled by phosphorylation. " Q9BYW1,"PROTEIN NAMES: Solute carrier family 2, facilitated glucose transporter member 11 (Glucose transporter type 11) (GLUT-11) PROTEIN FAMILY: Major facilitator superfamily, Sugar transporter family, Glucose transporter subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily. FUNCTION: Facilitative glucose transporter. KEYWORDS: Alternative splicing;Cell membrane;Glycoprotein;Membrane;Reference proteome;Sugar transport;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein " Q9BZ11,PROTEIN NAMES: Disintegrin and metalloproteinase domain-containing protein 33 (ADAM 33) ORGANISM: Homo sapiens (Human) KEYWORDS: 3D-structure;Alternative splicing;Asthma;Cleavage on pair of basic residues;Disulfide bond;EGF-like domain;Glycoprotein;Hydrolase;Membrane;Metal-binding;Metalloprotease;Protease;Reference proteome;Signal;Transmembrane;Transmembrane helix;Zinc;Zymogen SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. MISCELLANEOUS: [Isoform 3]: By similarity with mouse isoform. Q9BZ23,"PROTEIN NAMES: Pantothenate kinase 2, mitochondrial (hPanK2) (Pantothenic acid kinase 2) [Cleaved into: Pantothenate kinase 2, mitochondrial intermediate form (iPanK2); Pantothenate kinase 2, mitochondrial mature form (mPanK2)] PROTEIN FAMILY: Type II pantothenate kinase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the type II pantothenate kinase family. FUNCTION: [Isoform 1]: Mitochondrial isoform that catalyzes the phosphorylation of pantothenate to generate 4'-phosphopantothenate in the first and rate-determining step of coenzyme A (CoA) synthesis. Required for angiogenic activity of umbilical vein of endothelial cells (HUVEC).; FUNCTION: [Isoform 4]: Cytoplasmic isoform that catalyzes the phosphorylation of pantothenate to generate 4'-phosphopantothenate in the first and rate-determining step of coenzyme A (CoA) synthesis. PATHWAY: Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 1/5. KEYWORDS: 3D-structure;Alternative initiation;Alternative splicing;Angiogenesis;ATP-binding;Coenzyme A biosynthesis;Cytoplasm;Direct protein sequencing;Disease variant;Kinase;Mitochondrion;Neurodegeneration;Nucleotide-binding;Nucleus;Phosphoprotein;Reference proteome;Transferase;Transit peptide SUBCELLULAR LOCATION: [Isoform 1]: Mitochondrion Mitochondrion intermembrane space Nucleus Note=Localizes predominantly to the mitochondria and to a lesser extent to the nucleus. Found in both the mitochondria and the nucleus throughout the cell cycle, with the exception of the G2/M phase when it is restricted to mitochdondria.; SUBCELLULAR LOCATION: [Isoform 2]: Cytoplasm.; SUBCELLULAR LOCATION: [Isoform 3]: Cytoplasm.; SUBCELLULAR LOCATION: [Isoform 4]: Cytoplasm MISCELLANEOUS: The HSS syndrome has been proposed to be renamed because of the unethical activities of Julius Hallervorden and Hugo Spatz during world war II.; MISCELLANEOUS: [Isoform 2]: Produced by alternative initiation at Met-124 of isoform 1.; MISCELLANEOUS: [Isoform 4]: May be produced by alternative initiation at Leu-111 of isoform 1." Q9BZE9,PROTEIN NAMES: Tether containing UBX domain for GLUT4 (Alveolar soft part sarcoma chromosomal region candidate gene 1 protein) (Alveolar soft part sarcoma locus) (Renal papillary cell carcinoma protein 17) (UBX domain-containing protein 9) ORGANISM: Homo sapiens (Human) FUNCTION: Tethering protein that sequesters GLUT4-containing vesicles in the cytoplasm in the absence of insulin. Modulates the amount of GLUT4 that is available at the cell surface (By similarity). Enhances VCP methylation catalyzed by VCPKMT. KEYWORDS: 3D-structure;Acetylation;Alternative splicing;Chromosomal rearrangement;Cytoplasm;Membrane;Nucleus;Phosphoprotein;Proto-oncogene;Reference proteome SUBCELLULAR LOCATION: Endomembrane system ; Peripheral membrane protein Endoplasmic reticulum-Golgi intermediate compartment membrane ; Peripheral membrane protein. Cytoplasm Nucleus Q9BZG2,PROTEIN NAMES: Testicular acid phosphatase (Acid phosphatase 4) PROTEIN FAMILY: Histidine acid phosphatase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the histidine acid phosphatase family. FUNCTION: May dephosphorylate receptor tyrosine-protein kinase ERBB4 and inhibits its ligand-induced proteolytic cleavage. May play a role in odontogenesis. KEYWORDS: Alternative splicing;Amelogenesis imperfecta;Disease variant;Disulfide bond;Glycoprotein;Hydrolase;Membrane;Reference proteome;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Single-pass type I membrane protein Q9BZJ7,PROTEIN NAMES: G-protein coupled receptor 62 (G-protein coupled receptor GPCR8) (hGPCR8) (G-protein coupled receptor KPG_005) PROTEIN FAMILY: G-protein coupled receptor 1 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the G-protein coupled receptor 1 family. FUNCTION: Orphan G-protein coupled receptor. Constitutively activates the G(q/11)/inositol phosphate and the G(s)-alpha/cAMP signaling pathways. Has spontaneous activity for beta-arrestin recruitment. Shows a reciprocal modulation of signaling functions with the melatonin receptor MTNR1B most likely through receptor heteromerization. KEYWORDS: Cell membrane;Endosome;G-protein coupled receptor;Glycoprotein;Membrane;Receptor;Reference proteome;Transducer;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Endosome membrane ; Multi-pass membrane protein Note=Colocalizes with ARRB2 in the endosome. Q9BZQ2,"PROTEIN NAMES: Testicular spindle-associated protein SHCBP1L (SHC SH2 domain-binding protein 1-like protein) ORGANISM: Homo sapiens (Human) FUNCTION: Testis-specific spindle-associated factor that plays a role in spermatogenesis. In association with HSPA2, participates in the maintenance of spindle integrity during meiosis in male germ cells. KEYWORDS: Acetylation;Alternative splicing;Coiled coil;Cytoplasm;Cytoskeleton;Differentiation;Phosphoprotein;Reference proteome;Repeat;Spermatogenesis SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, spindle Note=Colocalizes with alpha tubulin during meiosis. Colocalizes with HSPA2 at spindle during the meiosis process. " Q9BZY9,"PROTEIN NAMES: E3 ubiquitin-protein ligase TRIM31 (Tripartite motif-containing protein 31) PROTEIN FAMILY: TRIM/RBCC family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the TRIM/RBCC family. FUNCTION: E3 ubiquitin-protein ligase that acts as a regulator of antiviral immune response and inflammation by mediating ubiquitination of substrates. Acts as a regulator of innate immune defense against viruses by mediating 'Lys-63'-linked ubiquitination of MAVS, promoting MAVS polymerization and formation of three-stranded helical filaments on mitochondria. Acts as a negative regulator of the NLRP3 inflammasome by catalyzing 'Lys-48'-linked ubiquitination of NLRP3, leading to its degradation. Regulator of Src-induced anchorage independent cell growth (By similarity). PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: 3D-structure;Alternative splicing;Antiviral defense;Coiled coil;Cytoplasm;Immunity;Inflammatory response;Innate immunity;Metal-binding;Mitochondrion;Reference proteome;Transferase;Ubl conjugation;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cytoplasm Mitochondrion Note=Predominantly expressed in the cytoplasm but a fraction is associated with the mitochondria. Recruited t omitochondria after viral infection. MISCELLANEOUS: [Isoform Beta]: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay." Q9C029,"PROTEIN NAMES: E3 ubiquitin-protein ligase TRIM7 (Glycogenin-interacting protein) (RING finger protein 90) (Tripartite motif-containing protein 7) PROTEIN FAMILY: TRIM/RBCC family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the TRIM/RBCC family. FUNCTION: E3 ubiquitin-protein ligase that have both tumor-promoting and tumor-suppressing activities and functions in several biological processes including innate immunity, regulation of ferroptosis as well as cell proliferation and migration. Acts as an antiviral effector against multiple viruses by targeting specific viral proteins for ubiquitination and degradation including norovirus NTPase protein or SARS-CoV-2 NSP5 and NSP8 proteins. Mechanistically, recognizes the C-terminal glutamine-containing motif usually generated by viral proteases that process the polyproteins and trigger their ubiquitination and subsequent degradation. Mediates 'Lys-63'-linked polyubiquitination and stabilization of the JUN coactivator RNF187 in response to growth factor signaling via the MEK/ERK pathway, thereby regulating JUN transactivation and cellular proliferation. Promotes the TLR4-mediated signaling activation through its E3 ligase domain leading to production of pro-inflammatory cytokines and type I interferon (By similarity). Also plays a negative role in the regulation of exogenous cytosolic DNA virus-triggered immune response. Mechanistically, enhances the 'Lys-48'-linked ubiquitination of STING1 leading to its proteasome-dependent degradation. Mediates the ubiquitination of the SIN3-HDAC chromatin remodeling complex component BRMS1. Modulates NCOA4-mediated ferritinophagy and ferroptosis in glioblastoma cells by ubiquitinating NCOA4, leading to its degradation.; FUNCTION: (Microbial infection) Promotes Zika virus replication by mediating envelope protein E ubiquitination. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: 3D-structure;Alternative splicing;Antiviral defense;Coiled coil;Cytoplasm;Golgi apparatus;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Transferase;Ubl conjugation;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Cytoplasm Golgi apparatus " Q9C037,"PROTEIN NAMES: E3 ubiquitin-protein ligase TRIM4 (RING finger protein 87) (RING-type E3 ubiquitin transferase TRIM4) (Tripartite motif-containing protein 4) PROTEIN FAMILY: TRIM/RBCC family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the TRIM/RBCC family. FUNCTION: E3 ubiquitin-protein ligase. Mediates 'Lys-63'-linked polyubiquitination of the innate immune receptor RIGI, this linkage doesn't lead to proteasomal degradation but seems to enhance IFN induction. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Alternative splicing;Coiled coil;Cytoplasm;Immunity;Innate immunity;Metal-binding;Reference proteome;Transferase;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cytoplasm " Q9C0U7,PROTEIN NAMES: Vacuolar protein sorting-associated protein vps5 PROTEIN FAMILY: Sorting nexin family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the sorting nexin family. FUNCTION: Required for efficient sporulation target of PtdIns(3)P in vesicle transport required for onset of the forespore membrane formation.; FUNCTION: Plays a role in vesicular protein sorting. Required for the endosome-to-Golgi retrieval of the vacuolar protein sorting receptor pep1/vps10. Component of the membrane-associated retromer complex which is essential in endosome-to-Golgi retrograde transport. The vps29-vps26-vps35 subcomplex may be involved in cargo selection. KEYWORDS: Cytoplasm;Golgi apparatus;Membrane;Phosphoprotein;Protein transport;Reference proteome;Sporulation;Transport SUBCELLULAR LOCATION: Cytoplasm Golgi apparatus Membrane ; Peripheral membrane protein ; Cytoplasmic side Q9C103,"PROTEIN NAMES: Crossover junction endonuclease eme1 (Essential meiotic endonuclease 1) PROTEIN FAMILY: EME1/MMS4 family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the EME1/MMS4 family. FUNCTION: Interacts with mus81 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, D-loops, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication fork intermediates. May be required in meiosis for the repair of meiosis-specific double strand breaks subsequent to single-end invasion (SEI). KEYWORDS: Direct protein sequencing;DNA damage;DNA recombination;DNA repair;Endonuclease;Hydrolase;Magnesium;Meiosis;Metal-binding;Nuclease;Nucleus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: S.pombe appears to be critically dependent on the mus81-eme1 endonuclease for the resolution of meiotic crossovers. This may be due to the absence of an alternate pathway for crossover resolution such as the MSH4-MSH5 pathway which exists in S.cerevisiae and other eukaryotes." Q9C107,"PROTEIN NAMES: Minichromosome loss protein 1 (DNA polymerase alpha accessory factor Mcl1) ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) FUNCTION: Has a role in regulating DNA replication complexes. Acts as a regulator of post DNA replication initiation. Associates with chromatin during G1 and S phases of mitosis. Required for the transcriptional repression of the outer repeats of the centromeric region. Acts as a polymerase alpha replication accessory factor and is important for S-phase DNA damage survival. Plays a role in lagging-strand synthesis and Ozaki fragment processing, in addition to DNA repair. KEYWORDS: Chromosome;DNA damage;DNA repair;DNA replication;Nucleus;Reference proteome;Repeat;Transcription;Transcription regulation;WD repeat SUBCELLULAR LOCATION: Nucleus. Chromosome. " Q9C1W3,"PROTEIN NAMES: Squalene epoxidase erg1 (SE) (Ergosterol biosynthetic protein 1) (Squalene monooxygenase erg1) PROTEIN FAMILY: Squalene monooxygenase family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the squalene monooxygenase family. FUNCTION: Squalene epoxidase; part of the third module of ergosterol biosynthesis pathway that includes by the late steps of the pathway. Erg1 catalyzes the epoxidation of squalene into 2,3-epoxysqualene. The third module or late pathway involves the ergosterol synthesis itself through consecutive reactions that mainly occur in the endoplasmic reticulum (ER) membrane. Firstly, the squalene synthase erg9 catalyzes the condensation of 2 farnesyl pyrophosphate moieties to form squalene, which is the precursor of all steroids. Secondly, squalene is converted into lanosterol by the consecutive action of the squalene epoxidase erg1 and the lanosterol synthase erg7. The lanosterol 14-alpha-demethylase erg11/cyp1 catalyzes C14-demethylation of lanosterol to produce 4,4'-dimethyl cholesta-8,14,24-triene-3-beta-ol. In the next steps, a complex process involving various demethylation, reduction and desaturation reactions catalyzed by the C-14 reductase erg24 and the C-4 demethylation complex erg25-erg26-erg27 leads to the production of zymosterol. Erg28 likely functions in the C-4 demethylation complex reaction by tethering erg26 and Erg27 to the endoplasmic reticulum or to facilitate interaction between these proteins. Then, the sterol 24-C-methyltransferase erg6 catalyzes the methyl transfer from S-adenosyl-methionine to the C-24 of zymosterol to form fecosterol. The C-8 sterol isomerase erg2 catalyzes the reaction which results in unsaturation at C-7 in the B ring of sterols and thus converts fecosterol to episterol. The sterol-C5-desaturases erg31 and erg32 then catalyze the introduction of a C-5 double bond in the B ring to produce 5-dehydroepisterol. The C-22 sterol desaturase erg5 further converts 5-dehydroepisterol into ergosta-5,7,22,24(28)-tetraen-3beta-ol by forming the C-22(23) double bond in the sterol side chain. Finally, ergosta-5,7,22,24(28)-tetraen-3beta-ol is substrate of the C-24(28) sterol reductase erg4 to produce ergosterol (Probable). In the genus Schizosaccharomyces, a second route exists between lanosterol and fecosterol, via the methylation of lanosterol to eburicol by erg6, followed by C14-demethylation by erg11/cyp1 and C4-demethylation by the demethylation complex erg25-erg26-erg27 (Probable). PATHWAY: Terpene metabolism; lanosterol biosynthesis; lanosterol from farnesyl diphosphate: step 2/3.; PATHWAY: Steroid metabolism; ergosterol biosynthesis. KEYWORDS: Endoplasmic reticulum;FAD;Flavoprotein;Membrane;Microsome;Oxidoreductase;Reference proteome;Transmembrane;Transmembrane helix;Vacuole SUBCELLULAR LOCATION: Microsome membrane ; Multi-pass membrane protein Endoplasmic reticulum membrane ; Multi-pass membrane protein Vacuole membrane ; Multi-pass membrane protein MISCELLANEOUS: In Aspergillus, the biosynthesis pathway of the sterol precursors leading to the prevalent sterol ergosterol differs from yeast. The ringsystem of lanosterol in S.cerevisiae is firstly demethylised in three enzymatic steps leading to the intermediate zymosterol and secondly a methyl group is added to zymosterol by the sterol 24-C-methyltransferase to form fecosterol. In Aspergillus, lanosterol is firstly transmethylated by the sterol 24-C-methyltransferase leading to the intermediate eburicol and secondly demethylated in three steps to form fecosterol. In the genus Schizosaccharomyces, 2 routes exist from lanosterol to erposterol: the classical one via zymosterol and the second one via the formation of eburicol followed by demethylation." Q9C550,"PROTEIN NAMES: 2-isopropylmalate synthase 2, chloroplastic (2-isopropylmalate synthase 1) (Methylthioalkylmalate synthase-like 3) PROTEIN FAMILY: Alpha-IPM synthase/homocitrate synthase family, LeuA type 1 subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily. FUNCTION: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate). Involved in Leu biosynthesis, but does not participate in the chain elongation of glucosinolates. PATHWAY: Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 1/4. KEYWORDS: Amino-acid biosynthesis;Branched-chain amino acid biosynthesis;Chloroplast;Leucine biosynthesis;Lyase;Metal-binding;Plastid;Reference proteome;Transferase;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast " Q9C566,"PROTEIN NAMES: Peptidyl-prolyl cis-trans isomerase CYP40 (PPIase CYP40) (Cyclophilin of 40 kDa) (AtCYP40) (Cyclophilin-40) (Protein SQUINT) (Rotamase CYP40) PROTEIN FAMILY: Cyclophilin-type PPIase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the cyclophilin-type PPIase family. FUNCTION: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Involved in promoting the expression of the juvenile phase of vegetative development, and, to a lower extent, in regulating the positioning of floral buds, floral morphogenesis and the expression of HSPs. Collaboratively with RBL and ULT1, influences floral meristem (FM) determinacy in an AGAMOUS and SUPERMAN-dependent manner, thus contributing to the floral developmental homeostasis. KEYWORDS: Chaperone;Cytoplasm;Isomerase;Reference proteome;Repeat;Rotamase;TPR repeat SUBCELLULAR LOCATION: Cytoplasm " Q9C5J9,"PROTEIN NAMES: Small GTPase LIP1 (Protein LIGHT INSENSITIVE PERIOD 1) PROTEIN FAMILY: Small GTPase superfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the small GTPase superfamily. FUNCTION: Functional small GTPase that acts as a negative factor controlling the light-dependent period shortening of circadian rhythms and light-induced phase resetting during the subjective night. May protect the clock from excessive or mistimed light. Suppresses red and blue light-mediated photomorphogenesis and is required for light-controlled inhibition of endoreplication and tolerance to salt stress. The entrainment of the circadian clock is independent from the other pleiotropic effects. Could be a regulator of seedling establishment. KEYWORDS: Cytoplasm;GTP-binding;Nucleotide-binding;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus Cytoplasm Note=The localization is not affected by light, but the nuclear localization is essential for the circadian function. MISCELLANEOUS: Has a GTPase activity despite the replacement of the highly conserved Glu-94 for His." Q9C5Q9,PROTEIN NAMES: Protein PHLOEM PROTEIN 2-LIKE A5 (AtPP2-A5) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) KEYWORDS: Alternative splicing;Hydrolase;NAD;Reference proteome Q9C5T4,"PROTEIN NAMES: WRKY transcription factor 18 (WRKY DNA-binding protein 18) (AtWRKY18) PROTEIN FAMILY: WRKY group II-a family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the WRKY group II-a family. FUNCTION: Transcription factor. Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element. Positively modulates defense-related gene expression and disease resistance. KEYWORDS: 3D-structure;Alternative splicing;DNA-binding;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: Constitutive expression at high level causes severe abnormality in plant growth." Q9C5W7,"PROTEIN NAMES: Tobamovirus multiplication protein 2A (AtTOM2A) PROTEIN FAMILY: Tetraspanin (TM4SF) family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the tetraspanin (TM4SF) family. FUNCTION: Necessary for the efficient intracellular multiplication of tobamoviruses, being a component of the replication complex. KEYWORDS: Membrane;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix;Vacuole SUBCELLULAR LOCATION: Vacuole membrane ; Multi-pass membrane protein " Q9C5Z2,"PROTEIN NAMES: Eukaryotic translation initiation factor 3 subunit H (eIF3h) (Eukaryotic translation initiation factor 3 subunit 3) (eIF-3-gamma) (eIF3 p38 subunit) PROTEIN FAMILY: EIF-3 subunit H family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the eIF-3 subunit H family. FUNCTION: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation (Potential). Regulates translation initiation of specific 5' mRNAs harboring multiple upstream open reading frames (uORFs) in their 5' leader sequence (e.g. BETA-OHASE 2 and LHY). KEYWORDS: Abscisic acid signaling pathway;Alternative splicing;Cytoplasm;Initiation factor;Phosphoprotein;Protein biosynthesis;Reference proteome;Translation regulation SUBCELLULAR LOCATION: Cytoplasm " Q9C658,"PROTEIN NAMES: Protein decapping 5 PROTEIN FAMILY: LSM14 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the LSM14 family. FUNCTION: As a component of the decapping complex, involved in the degradation of mRNAs. Promotes P-body formation. Translational repressor. KEYWORDS: Alternative splicing;Cytoplasm;mRNA processing;Reference proteome;Repressor;Translation regulation SUBCELLULAR LOCATION: Cytoplasm, P-body " Q9C667,"PROTEIN NAMES: Chaperonin 60 subunit beta 4, chloroplastic (CPN-60 beta 4) PROTEIN FAMILY: Chaperonin (HSP60) family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the chaperonin (HSP60) family. FUNCTION: Involved specifically in the folding of NDHH, a subunit of the chloroplast NADH dehydrogenase-like complex (NDH). KEYWORDS: Alternative splicing;ATP-binding;Chaperone;Chloroplast;Coiled coil;Nucleotide-binding;Plastid;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast stroma MISCELLANEOUS: CPN60B1, CPN60B2 or CPN60B3 cannot complement the function of CPN60B4.; MISCELLANEOUS: Assisted protein folding requires ATP hydrolysis, but not K(+) ions." Q9C778,"PROTEIN NAMES: Elongator complex protein 4 (AtELP4) (Elongator component 4) (Protein ELONGATA 1) PROTEIN FAMILY: ELP4 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the ELP4 family. FUNCTION: Component of the elongator complex which is required for multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl uridine), mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U (5-carbamoylmethyl uridine) (By similarity). The elongator complex catalyzes the formation of carboxymethyluridine in the wobble base at position 34 in tRNAs (By similarity). Promotes organ development by modulating cell division rate. May regulate mechanisms producing carbon assimilates or importing sucrose. Involved in the repression of the abscisic acid (ABA) signaling pathway during seed germination and seedling growth. Required for auxin distribution or signaling. Involved in oxidative stress signaling. Prevents anthocyanin accumulation. PATHWAY: tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis. KEYWORDS: Abscisic acid signaling pathway;Alternative splicing;Auxin signaling pathway;Cytoplasm;Developmental protein;Nucleus;Reference proteome;tRNA processing SUBCELLULAR LOCATION: Cytoplasm Nucleus " Q9C7C4,"PROTEIN NAMES: Protein EMSY-LIKE 1 (AtEML1) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Probably involved in the regulation of chromatin states (Probable). Contributes to RPP7-mediated and basal immunity, especially against Hyaloperonospora arabidopsidis isolate Hiks1. Regulates negatively EDM2-dependent floral transition. KEYWORDS: Alternative splicing;Coiled coil;Nucleus;Phosphoprotein;Plant defense;Reference proteome SUBCELLULAR LOCATION: Nucleus " Q9C7F7,"PROTEIN NAMES: Non-specific lipid transfer protein GPI-anchored 1 (AtLTPG-1) (Protein LTP-GPI-ANCHORED 1) PROTEIN FAMILY: Plant LTP family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the plant LTP family. FUNCTION: Lipid transfer protein that, together with LTPG2, binds to lipids and functions as a component of the cuticular lipid export machinery that performs extensive export of intracellular lipids (e.g. C29 alkane) from epidermal cells to the surface to build the cuticular wax layer and silique walls. Involved in the establishment of resistance to the necrotrophic fungal pathogen Alternaria brassicicola. Contributes to pre-invasive defense against some non-host powdery mildew pathogens by preventing the penetration of the epidermal cell wall by the fungal agents (e.g. Blumeria graminis f. sp. hordei (Bgh)). Maybe involved in seed and ovule maturation and development, probably by regulating the fatty acids homeostasis during suberin and sporopollenin biosynthesis or deposition (By similarity). KEYWORDS: Cell membrane;Cell wall;Disulfide bond;Endoplasmic reticulum;Glycoprotein;Golgi apparatus;GPI-anchor;Lipid-binding;Lipoprotein;Membrane;Plant defense;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Cell membrane ; Lipid-anchor, GPI-anchor Secreted, cell wall Endoplasmic reticulum Golgi apparatus Note=Targeted to the plasma membrane via the vesicular trafficking system. Localized to the plasma membrane on all faces of epidermal cells. Also detected in the periclinal cell wall. In young meristematic cells, observed in plasma membrane and in puncta resembling the Golgi. Localized to papillae at the sites of Blumeria graminis f. sp. hordei (Bgh) penetration (Bgh). " Q9C7T7,"PROTEIN NAMES: Receptor protein-tyrosine kinase CEPR2 (Protein C-TERMINALLY ENCODED PEPTIDE RECEPTOR 2) PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. FUNCTION: Receptor kinase involved in the perception of C-terminally encoded plant signaling peptide (CEP) and subsequent regulation of root and shoot development. Together with CEPR1, mediates systemic nitrogen (N)-demand signaling upon the perception of root-derived peptides (e.g. CEP1) via the up-regulation of genes involved in N uptake and assimilation pathways. KEYWORDS: ATP-binding;Cell membrane;Glycoprotein;Kinase;Leucine-rich repeat;Membrane;Nucleotide-binding;Phosphoprotein;Receptor;Reference proteome;Repeat;Serine/threonine-protein kinase;Signal;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass membrane protein " Q9C7U7,"PROTEIN NAMES: Transcription factor MYB20 (Myb-related protein 20) (AtMYB20) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Transcription factor that acts as a positive regulator of abscisic acid (ABA) signaling in response to salt stress. Acts as a negative regulator ABI1, ABI2 and PP2CA, which are protein phosphatases 2C acting as negative regulator of ABA signaling. Binds to the DNA specific sequence and core element 5'-ACGT-3' found in the promoters of ABI1 and PP2CA to negatively regulate their expression during ABA-dependent salt stress response. KEYWORDS: DNA-binding;Nucleus;Reference proteome;Repeat;Stress response;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: Plants overexpressing MYB20 display increased tolerance to salt stress." Q9C895,"PROTEIN NAMES: E3 ubiquitin-protein ligase BRE1-like 2 (Protein HISTONE MONOUBIQUITINATION 2) (AtHUB2) (RING-type E3 ubiquitin transferase BRE1-like 2) PROTEIN FAMILY: BRE1 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the BRE1 family. FUNCTION: E3 ubiquitin-protein ligase that monoubiquitinates H2B to form H2BK143ub1. H2BK143ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for H3K4me and maybe H3K79me. It thereby plays a central role in histone code and gene regulation. Forms a ubiquitin ligase complex in cooperation with the E2 enzyme UBC2/RAD6. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Alternative splicing;Chromatin regulator;Coiled coil;Metal-binding;Nucleus;Reference proteome;Transferase;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: HUB1 and HUB2 are involved in the same processes, but are weakly or not redundant.; MISCELLANEOUS: [Isoform 2]: May be due to an intron retention." Q9C8L4,"PROTEIN NAMES: Persulfide dioxygenase ETHE1 homolog, mitochondrial (Glyoxalase II) (Glx II) (Sulfur dioxygenase ETHE1) PROTEIN FAMILY: Metallo-beta-lactamase superfamily, Glyoxalase II family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family. FUNCTION: Sulfur dioxygenase that plays an essential role in hydrogen sulfide catabolism in the mitochondrial matrix. Hydrogen sulfide (H(2)S) gives rise to cysteine persulfide residues. ETHE1 consumes molecular oxygen to catalyze the oxidation of the persulfide, once it has been transferred to a thiophilic acceptor, such as glutathione (R-SSH). Plays an important role in metabolic homeostasis in mitochondria by metabolizing hydrogen sulfide and preventing the accumulation of supraphysiological H(2)S levels that have toxic effects, due to the inhibition of cytochrome c oxidase. Required for normal endosperm development in seed, and thereby also required for normal embryo development. KEYWORDS: 3D-structure;Acetylation;Alternative splicing;Dioxygenase;Iron;Metal-binding;Mitochondrion;Oxidoreductase;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Mitochondrion " Q9C9C0,"PROTEIN NAMES: Serine protease SPPA, chloroplastic (Signal peptide peptidase SPPA) PROTEIN FAMILY: Peptidase S49 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the peptidase S49 family. FUNCTION: Serine protease that may be involved in the light-dependent degradation of antenna and photosystem II in chloroplasts. May function during high light acclimation in plastids. KEYWORDS: Chloroplast;Hydrolase;Membrane;Plastid;Protease;Reference proteome;Serine protease;Stress response;Thylakoid;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast stroma Plastid, chloroplast thylakoid membrane Note=Mostly exposed to the stroma but behaves as an intrinsic membrane protein. " Q9C9C9,"PROTEIN NAMES: Cytosolic sulfotransferase 18 (AtSOT18) (Desulfo-glucosinolate sulfotransferase B) (Sulfotransferase 5B) (AtST5b) PROTEIN FAMILY: Sulfotransferase 1 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the sulfotransferase 1 family. FUNCTION: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of desulfo-glucosinolates (dsGSs), the final step in the biosynthesis of the glucosinolate core structure. Preferred substrate are the long-chain desulfo-glucosinolates, 7-methylthioheptyl and 8-methylthiooctyl, derived from methionine. Substrate preference is desulfo-benzyl glucosinolate > desulfo-4-methylthiobutyl glucosinolate > desulfo-6-methylthiohexyl glucosinolate > desulfo-3-methylthiopropyl glucosinolate > desulfo-indol-3-yl methyl glucosinolate > desulfo-singrin > desulfo-3-butenyl glucosinolate. KEYWORDS: 3D-structure;Acetylation;Cytoplasm;Reference proteome;Transferase SUBCELLULAR LOCATION: Cytoplasm MISCELLANEOUS: Strong differences in the kinetic behavior between the same protein from different cultivars." Q9C9Q8,PROTEIN NAMES: Probable pectin methyltransferase QUA2 (Protein OVERSENSITIVE TO SUGAR 1) (Protein QUASIMODO 2) (Protein TUMOROUS SHOOT DEVELOPMENT 2) PROTEIN FAMILY: Methyltransferase superfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the methyltransferase superfamily. FUNCTION: May be involved in the synthesis of homogalacturonan. Required for normal cell adhesion and plant development. PATHWAY: Glycan metabolism; pectin biosynthesis. KEYWORDS: Cell adhesion;Cell wall biogenesis/degradation;Glycoprotein;Golgi apparatus;Membrane;Methyltransferase;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus membrane ; Single-pass type II membrane protein MISCELLANEOUS: Co-expressed with the galacturonosyltransferase GAUT8/QUASIMODO1. Q9C9T2,"PROTEIN NAMES: Protein SAR DEFICIENT 1 PROTEIN FAMILY: Plant ACBP60 protein family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the plant ACBP60 protein family. FUNCTION: Transcription activator that binds DNA in a sequence-specific manner, 5'-GAAATTTTGG-3', to promote the expression of target genes. Recruited to the promoter of ICS1 and other defense-related genes (e.g. PR1 and SID2) in response to both biotic (e.g. Pseudomonas syringae pv. maculicola ES4326) and abiotic stresses (e.g. UV-B), thus triggering slow defense responses by stimulating salicylic acid (SA) biosynthesis. Required for basal and systemic acquired resistance to P. syringae pv. maculicola and Hyaloperonospora arabidopsidis. KEYWORDS: Activator;DNA-binding;Hypersensitive response;Nucleus;Plant defense;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " Q9C9Y6,"PROTEIN NAMES: Thioredoxin H9 (AtTrxh9) (Thioredoxin 9) (AtTRX9) PROTEIN FAMILY: Thioredoxin family, Plant H-type subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the thioredoxin family. Plant H-type subfamily. FUNCTION: Probable thiol-disulfide oxidoreductase that may play a role in intercellular communication due to its ability to move from cell to cell. KEYWORDS: Cell membrane;Disulfide bond;Electron transport;Lipoprotein;Membrane;Myristate;Palmitate;Phosphoprotein;Redox-active center;Reference proteome;Transport SUBCELLULAR LOCATION: Cell membrane " Q9CA67,"PROTEIN NAMES: Geranylgeranyl diphosphate reductase, chloroplastic (Geranylgeranyl reductase) PROTEIN FAMILY: Geranylgeranyl reductase family, ChlP subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the geranylgeranyl reductase family. ChlP subfamily. FUNCTION: Catalyzes the reduction of geranylgeranyl diphosphate to phytyl diphosphate, providing phytol for both tocopherol and chlorophyll synthesis. PATHWAY: Porphyrin-containing compound metabolism; chlorophyll biosynthesis.; PATHWAY: Cofactor biosynthesis; tocopherol biosynthesis. KEYWORDS: Chlorophyll biosynthesis;Chloroplast;Membrane;NADP;Oxidoreductase;Photosynthesis;Plastid;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast membrane " Q9CAC4,"PROTEIN NAMES: Myosin-binding protein 2 ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Membrane-anchored myosin receptors that define a distinct, plant-specific transport vesicle compartment. KEYWORDS: Coiled coil;Membrane;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endomembrane system ; Single-pass membrane protein " Q9CAE4,PROTEIN NAMES: U11/U12 small nuclear ribonucleoprotein 31 kDa protein (U11/U12 snRNP 31 kDa protein) (U11/U12-31K) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: RNA chaperone required for proper U12 intron splicing and for normal growth and development of plants. Mainly responsible for meristem activity. Plays a role in regulating cell division. KEYWORDS: Metal-binding;mRNA processing;mRNA splicing;Nucleus;Reference proteome;Ribonucleoprotein;RNA-binding;Spliceosome;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Q9CAF5,"PROTEIN NAMES: ABC transporter I family member 6, chloroplastic (ABC transporter ABCI.6) (AtABCI6) (ABC transporter ATPase) (Non-intrinsic ABC protein 7) (AtNAP7) (Plastid SufC-like protein) PROTEIN FAMILY: ABC transporter superfamily, ABCI family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the ABC transporter superfamily. ABCI family. FUNCTION: Essential protein. Required during embryo development, especially at early stages. Involved in chloroplast differentiation. KEYWORDS: ATP-binding;Chloroplast;Nucleotide-binding;Plastid;Reference proteome;Transit peptide;Transport SUBCELLULAR LOCATION: Plastid, chloroplast " Q9CAT6,"PROTEIN NAMES: Organic cation/carnitine transporter 1 (AtOCT1) PROTEIN FAMILY: Major facilitator superfamily, Organic cation transporter family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the major facilitator (TC 2.A.1) superfamily. Organic cation transporter (TC 2.A.1.19) family. FUNCTION: High affinity carnitine transporter involved in the active cellular uptake of carnitine. Also transports organic cations. Regulates lateral root development. KEYWORDS: ATP-binding;Cell membrane;Developmental protein;Ion transport;Membrane;Nucleotide-binding;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein " Q9CQ76,PROTEIN NAMES: Nephrocan PROTEIN FAMILY: Small leucine-rich proteoglycan (SLRP) family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the small leucine-rich proteoglycan (SLRP) family. FUNCTION: May inhibit TGF-beta signaling. KEYWORDS: Glycoprotein;Leucine-rich repeat;Reference proteome;Repeat;Secreted;Signal SUBCELLULAR LOCATION: Secreted Q9CQM9,"PROTEIN NAMES: Glutaredoxin-3 (PKC-interacting cousin of thioredoxin) (PICOT) (PKC-theta-interacting protein) (PKCq-interacting protein) (Thioredoxin-like protein 2) ORGANISM: Mus musculus (Mouse) FUNCTION: Together with BOLA2, acts as a cytosolic iron-sulfur (Fe-S) cluster assembly factor that facilitates [2Fe-2S] cluster insertion into a subset of cytosolic proteins (By similarity). Acts as a critical negative regulator of cardiac hypertrophy and a positive inotropic regulator. Required for hemoglobin maturation. Does not possess any thyoredoxin activity since it lacks the conserved motif that is essential for catalytic activity (By similarity). KEYWORDS: 3D-structure;Acetylation;Cytoplasm;Direct protein sequencing;Iron;Iron-sulfur;Metal-binding;Phosphoprotein;Reference proteome;Repeat SUBCELLULAR LOCATION: Cytoplasm, cytosol Cytoplasm, cell cortex Cytoplasm, myofibril, sarcomere, Z line Note=Under the plasma membrane. After PMA stimulation, GLRX3 and PRKCQ/PKC-theta translocate to a more extended submembrane area. In the Z line, found associated with CSRP3. MISCELLANEOUS: Transgenic mice with cardiac-specific Glrx3 overexpression show that it is a potent inhibitor of cardiac hypertrophy induced by pressure overload (transverse aortic constriction). In addition, overexpression dramatically increases the ventricular function and cardiomyocyte contractility." Q9CR14,"PROTEIN NAMES: E3 ubiquitin-protein ligase FANCL (Fanconi anemia group L protein homolog) (Proliferation of germ cells protein) (RING-type E3 ubiquitin transferase FANCL) ORGANISM: Mus musculus (Mouse) FUNCTION: Ubiquitin ligase protein that mediates monoubiquitination of FANCD2, a key step in the DNA damage pathway. Also mediates monoubiquitination of FANCI. May stimulate the ubiquitin release from UBE2W. May be required for proper primordial germ cell proliferation in the embryonic stage, whereas it is probably not needed for spermatogonial proliferation after birth. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Alternative splicing;Cytoplasm;DNA damage;DNA repair;Metal-binding;Nucleus;Reference proteome;Transferase;Ubl conjugation;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cytoplasm Nucleus Note=In the nucleus, colocalizes with UBE2W. " Q9CWI3,"PROTEIN NAMES: BRCA2 and CDKN1A-interacting protein PROTEIN FAMILY: BCP1 family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the BCP1 family. FUNCTION: During interphase, required for microtubule organizing and anchoring activities. During mitosis, required for the organization and stabilization of the spindle pole. May promote cell cycle arrest by enhancing the inhibition of CDK2 activity by CDKN1A. May be required for repair of DNA damage by homologous recombination in conjunction with BRCA2. May not be involved in non-homologous end joining (NHEJ) (By similarity). KEYWORDS: Cell cycle;Cytoplasm;Cytoskeleton;DNA damage;DNA repair;Nucleus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Nucleus Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole Cytoplasm, cytoskeleton, spindle pole Note=Colocalizes with BRCA2 in discrete nuclear foci (By similarity). In interphase, preferential localizes to the mother centriole. Recruited to the spindle pole matrix and centrosome by microtubules and dynein/dynactin activity (By similarity). " Q9CWR0,"PROTEIN NAMES: Rho guanine nucleotide exchange factor 25 (Guanine nucleotide exchange factor GEFT) (Rac/Cdc42/Rho exchange factor GEFT) (RhoA/Rac/Cdc42 guanine nucleotide exchange factor GEFT) (p63RhoGEF) ORGANISM: Mus musculus (Mouse) FUNCTION: May play a role in actin cytoskeleton reorganization in different tissues since its activation induces formation of actin stress fibers. It works as a guanine nucleotide exchange factor for Rho family of small GTPases. Links specifically G alpha q/11-coupled receptors to RHOA activation (By similarity). May be an important regulator of processes involved in axon and dendrite formation. In neurons seems to be an exchange factor primarily for RAC1. Involved in skeletal myogenesis. KEYWORDS: Alternative splicing;Cell membrane;Cytoplasm;Guanine-nucleotide releasing factor;Membrane;Reference proteome SUBCELLULAR LOCATION: Cytoplasm, myofibril, sarcomere Cell membrane Cytoplasm, myofibril Note=Highly colocalizes with actin regions. " Q9CWW7,"PROTEIN NAMES: CXXC-type zinc finger protein 1 (CpG-binding protein) (PHD finger and CXXC domain-containing protein 1) ORGANISM: Mus musculus (Mouse) FUNCTION: Transcriptional activator that exhibits a unique DNA binding specificity for CpG unmethylated motifs with a preference for CpGG. KEYWORDS: Acetylation;Activator;Coiled coil;DNA-binding;Isopeptide bond;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation;Ubl conjugation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus speckle Nucleus Note=Associated with euchromatin. During mitosis, excluded from condensed chromosomes (By similarity). " Q9CYC6,"PROTEIN NAMES: m7GpppN-mRNA hydrolase (mRNA-decapping enzyme 2) PROTEIN FAMILY: Nudix hydrolase family, DCP2 subfamily ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the Nudix hydrolase family. DCP2 subfamily. FUNCTION: Decapping metalloenzyme that catalyzes the cleavage of the cap structure on mRNAs. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay (By similarity). Plays a role in replication-dependent histone mRNA degradation. Has higher activity towards mRNAs that lack a poly(A) tail. Has no activity towards a cap structure lacking an RNA moiety. The presence of a N(6)-methyladenosine methylation at the second transcribed position of mRNAs (N(6),2'-O-dimethyladenosine cap; m6A(m)) provides resistance to DCP2-mediated decapping (By similarity). Blocks autophagy in nutrient-rich conditions by repressing the expression of ATG-related genes through degradation of their transcripts (By similarity). KEYWORDS: Cytoplasm;Hydrolase;Manganese;Metal-binding;Nonsense-mediated mRNA decay;Nucleus;Phosphoprotein;Reference proteome;RNA-binding SUBCELLULAR LOCATION: Cytoplasm, P-body Nucleus Note=Predominantly cytoplasmic, in processing bodies (PB) (By similarity). A minor amount is nuclear (By similarity). " Q9CZW5,PROTEIN NAMES: Mitochondrial import receptor subunit TOM70 (Mitochondrial precursor proteins import receptor) (Translocase of outer membrane 70 kDa subunit) (Translocase of outer mitochondrial membrane protein 70) PROTEIN FAMILY: Tom70 family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the Tom70 family. FUNCTION: Acts as a receptor of the preprotein translocase complex of the outer mitochondrial membrane (TOM complex). Recognizes and mediates the translocation of mitochondrial preproteins from the cytosol into the mitochondria in a chaperone dependent manner (By similarity). Mediates TBK1 and IRF3 activation induced by MAVS in response to virus infection and promotes host antiviral responses during virus infection. KEYWORDS: Acetylation;Direct protein sequencing;Isopeptide bond;Membrane;Methylation;Mitochondrion;Mitochondrion outer membrane;Phosphoprotein;Reference proteome;Repeat;TPR repeat;Transmembrane;Transmembrane helix;Ubl conjugation SUBCELLULAR LOCATION: Mitochondrion outer membrane ; Single-pass membrane protein Q9D1N2,"PROTEIN NAMES: Glycosylphosphatidylinositol-anchored high density lipoprotein-binding protein 1 (GPI-HBP1) (GPI-anchored HDL-binding protein 1) (High density lipoprotein-binding protein 1) ORGANISM: Mus musculus (Mouse) FUNCTION: Mediates the transport of lipoprotein lipase LPL from the basolateral to the apical surface of endothelial cells in capillaries. Anchors LPL on the surface of endothelial cells in the lumen of blood capillaries. Thereby, plays an important role in lipolytic processing of chylomicrons by LPL, triglyceride metabolism and lipid homeostasis. Binds chylomicrons and phospholipid particles that contain APOA5. Binds high-density lipoprotein (HDL) and plays a role in the uptake of lipids from HDL. KEYWORDS: Cell membrane;Disulfide bond;Glycoprotein;GPI-anchor;Hyperlipidemia;Lipid-binding;Lipoprotein;Membrane;Reference proteome;Signal;Sulfation;Transport SUBCELLULAR LOCATION: Apical cell membrane ; Lipid-anchor, GPI-anchor Basolateral cell membrane ; Lipid-anchor, GPI-anchor Cell membrane ; Lipid-anchor, GPI-anchor " Q9D2N9,"PROTEIN NAMES: Vacuolar protein sorting-associated protein 33A PROTEIN FAMILY: STXBP/unc-18/SEC1 family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the STXBP/unc-18/SEC1 family. FUNCTION: Plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport and autophagic pathways. Believed to act as a core component of the putative HOPS and CORVET endosomal tethering complexes which are proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion. The HOPS complex is proposed to be recruited to Rab7 on the late endosomal membrane and to regulate late endocytic, phagocytic and autophagic traffic towards lysosomes. The CORVET complex is proposed to function as a Rab5 effector to mediate early endosome fusion probably in specific endosome subpopulations. Required for fusion of endosomes and autophagosomes with lysosomes; the function is dependent on its association with VPS16 but not VIPAS39. The function in autophagosome-lysosome fusion implicates STX17 but not UVRAG. KEYWORDS: Autophagy;Cytoplasmic vesicle;Endosome;Lysosome;Membrane;Protein transport;Reference proteome;Transport SUBCELLULAR LOCATION: Cytoplasmic vesicle Late endosome membrane ; Peripheral membrane protein ; Cytoplasmic side Lysosome membrane ; Peripheral membrane protein ; Cytoplasmic side Early endosome Cytoplasmic vesicle, autophagosome Cytoplasmic vesicle, clathrin-coated vesicle " Q9D4G2,"PROTEIN NAMES: Heat shock factor 2-binding protein ORGANISM: Mus musculus (Mouse) FUNCTION: Meiotic recombination factor component of recombination bridges involved in meiotic double-strand break repair. Modulates the localization of recombinases DMC1:RAD51 to meiotic double-strand break (DSB) sites through the interaction with BRCA2 and its recruitment during meiotic recombination. Indispensable for the DSB repair, homologous synapsis, and crossover formation that are needed for progression past metaphase I, is essential for spermatogenesis and male fertility. Required for proper recombinase recruitment in female meiosis. Inhibits BNC1 transcriptional activity during spermatogenesis, probably by sequestering it in the cytoplasm. May be involved in modulating HSF2 activation in testis (By similarity). KEYWORDS: 3D-structure;Chromosome;Coiled coil;Cytoplasm;Reference proteome;Ubl conjugation SUBCELLULAR LOCATION: Cytoplasm Chromosome Note=Localizes on double-strand breaks (DSBs) in mitotic and meiotic chromosomes. " Q9D5V6,"PROTEIN NAMES: Synapse-associated protein 1 (BSD domain-containing signal transducer and Akt interactor protein) (BSTA) ORGANISM: Mus musculus (Mouse) FUNCTION: Plays a role in adipocyte differentiation by promoting mTORC2-mediated phosphorylation of AKT1 at 'Ser-473' after growth factor stimulation. KEYWORDS: Cell membrane;Cell projection;Cytoplasm;Differentiation;Golgi apparatus;Membrane;Phosphoprotein;Postsynaptic cell membrane;Reference proteome;Synapse SUBCELLULAR LOCATION: Cytoplasm, perinuclear region Golgi apparatus Perikaryon Cell projection, axon Cell projection, dendrite Cell projection, growth cone Presynaptic cell membrane Postsynaptic cell membrane Membrane Note=Localizes to cholinergic neuromuscular junctions and in actin-rich growth cone regions. Membrane-associated in a epidermal growth factor (EGF)-dependent manner (By similarity). " Q9D7I8,"PROTEIN NAMES: Protein FAM83D PROTEIN FAMILY: FAM83 family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the FAM83 family. FUNCTION: Through the degradation of FBXW7, may act indirectly on the expression and downstream signaling of MTOR, JUN and MYC (By similarity). May play also a role in cell proliferation through activation of the ERK1/ERK2 signaling cascade (By similarity). May also be important for proper chromosome congression and alignment during mitosis through its interaction with KIF22 (By similarity). KEYWORDS: Alternative splicing;Cell cycle;Cell division;Cytoplasm;Cytoskeleton;Mitosis;Phosphoprotein;Proto-oncogene;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Cytoplasm, cytoskeleton, spindle Cytoplasm, cytoskeleton, spindle pole Note=Primarily cytoplasmic during interphase, but at prophase, associates with spindle microtubules, with a clear concentration toward the spindle poles. It persists on spindle microtubules through metaphase and anaphase. " Q9D7S0,"PROTEIN NAMES: Ly6/PLAUR domain-containing protein 8 PROTEIN FAMILY: CNF-like-inhibitor family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the CNF-like-inhibitor family. FUNCTION: Secreted protein specifically required to prevent invasion of Gram-negative bacteria in the inner mucus layer of the colon epithelium, a portion of the large intestine which is free of commensal microbiota. Prevents invasion of flagellated microbiota by binding to the flagellum of bacteria, such as P.mirabilis, thereby inhibiting bacterial motility in the intestinal lumen. Segregation of intestinal bacteria and epithelial cells in the colon is required to preserve intestinal homeostasis. KEYWORDS: Cell membrane;Glycoprotein;GPI-anchor;Lipoprotein;Membrane;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Cell membrane ; Lipid-anchor, GPI-anchor Secreted Note=Secreted into the lumen of the colon following cleavage of the GPI-anchor. " Q9D8B1,"PROTEIN NAMES: Androgen-induced gene 1 protein (AIG-1) (Fatty acid esters of hydroxy fatty acids hydrolase AIG1) (FAHFA hydrolase AIG1) PROTEIN FAMILY: AIG1 family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the AIG1 family. FUNCTION: Hydrolyzes bioactive fatty-acid esters of hydroxy-fatty acids (FAHFAs), but not other major classes of lipids (By similarity). Shows a preference for FAHFAs with branching distal from the carboxylate head group of the lipids (By similarity). KEYWORDS: Alternative splicing;Cell membrane;Hydrolase;Lipid metabolism;Membrane;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein " Q9D8N3,"PROTEIN NAMES: Lysoplasmalogenase TMEM86A (Transmembrane protein 86A) PROTEIN FAMILY: TMEM86 family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the TMEM86 family. FUNCTION: Catalyzes the hydrolysis of the vinyl ether bond of choline or ethanolamine lysoplasmalogens, forming fatty aldehyde and glycerophosphocholine or glycerophosphoethanolamine, respectively and is specific for the sn-2-deacylated (lyso) form of plasmalogen. Plays an important role in lysoplasmalogen metabolism in the adipocyte tissue and macrophages. KEYWORDS: Endoplasmic reticulum;Hydrolase;Lipid metabolism;Membrane;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein " Q9D9J7,"PROTEIN NAMES: Izumo sperm-egg fusion protein 1 (Oocyte binding/fusion factor) (OBF) (Sperm-specific protein izumo) PROTEIN FAMILY: Izumo family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the Izumo family. FUNCTION: Essential sperm cell-surface protein required for fertilization by acting as a ligand for IZUMO1R/JUNO receptor on egg. The IZUMO1:IZUMO1R/JUNO interaction is a necessary adhesion event between sperm and egg that is required for fertilization but is not sufficient for cell fusion. The ligand-receptor interaction probably does not act as a membrane 'fusogen'. KEYWORDS: 3D-structure;Cell membrane;Cytoplasmic vesicle;Disulfide bond;Fertilization;Glycoprotein;Immunoglobulin domain;Membrane;Phosphoprotein;Reference proteome;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein Cytoplasmic vesicle, secretory vesicle, acrosome membrane ; Single-pass type I membrane protein Note=Localizes initially to the acrosome membrane of the sperm head (both outer and inner acrosomal membranes). During the acrosome reaction, translocates to the plasma membrane. MISCELLANEOUS: Izumo is the name of a Japanese shrine to marriage." Q9D9T8,"PROTEIN NAMES: EF-hand domain-containing protein 1 (Myoclonin-1) ORGANISM: Mus musculus (Mouse) FUNCTION: Microtubule-associated protein which regulates cell division and neuronal migration during cortical development. Necessary for mitotic spindle organization. Necessary for radial and tangential cell migration during brain development, possibly acting as a regulator of cell morphology and process formation during migration (By similarity). May enhance calcium influx through CACNA1E and stimulate programmed cell death. Overexpression of EFHC1 in hippocampal primary culture neurons induced apoptosis. Microtubule inner protein (MIP) part of the dynein-decorated doublet microtubules (DMTs) in cilia axoneme, which is required for motile cilia beating (By similarity). KEYWORDS: 3D-structure;Cell projection;Cytoplasm;Cytoskeleton;Reference proteome;Repeat SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Cytoplasm, cytoskeleton, spindle Cytoplasm, cytoskeleton, spindle pole Cytoplasm, cytoskeleton, cilium axoneme " Q9D9V2,"PROTEIN NAMES: Equatorin (Acrosome formation-associated factor) (MN9 antigen) ORGANISM: Mus musculus (Mouse) FUNCTION: Acrosomal membrane-anchored protein involved in the process of fertilization and in acrosome biogenesis. KEYWORDS: Alternative splicing;Cytoplasm;Cytoplasmic vesicle;Glycoprotein;Membrane;Nucleus;Phosphoprotein;Reference proteome;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: [Isoform 1]: Cytoplasmic vesicle, secretory vesicle, acrosome membrane; Single-pass type I membrane protein. Cytoplasmic vesicle, secretory vesicle, acrosome inner membrane; Single-pass type I membrane protein. Cytoplasmic vesicle, secretory vesicle, acrosome outer membrane; Single-pass type I membrane protein. Note=In the anterior acrosome region, enriched on the inner acrosomal membrane but minimal on the outer acrosomal membrane; in contrast in the posterior acrosome region enriched on both the inner and outer acrosomal membranes.; SUBCELLULAR LOCATION: [Isoform 2]: Nucleus. Cytoplasm. " Q9D9X8,"PROTEIN NAMES: Sperm acrosome membrane-associated protein 3 (Lysozyme-like protein 3) (Sperm lysozyme-like protein 1) (mSLLP1) [Cleaved into: Sperm acrosome membrane-associated protein 3, membrane form; Sperm acrosome membrane-associated protein 3, processed form] PROTEIN FAMILY: Glycosyl hydrolase 22 family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the glycosyl hydrolase 22 family. FUNCTION: Sperm surface membrane protein that may be involved in sperm-egg plasma membrane adhesion and fusion during fertilization. It could be a potential receptor for the egg oligosaccharide residue N-acetylglucosamine, which is present in the extracellular matrix over the egg plasma membrane. The processed form has no detectable bacteriolytic activity in vitro (By similarity). KEYWORDS: 3D-structure;Alternative splicing;Cytoplasmic vesicle;Disulfide bond;Membrane;Reference proteome;Secreted;Signal-anchor;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, acrosome membrane ; Single-pass type II membrane protein Note=Anterior acrosome in non-capacitated spermatozoa and retained in the equatorial segment and in the luminal face of both the inner and outer acrosomal membranes following capacitation and the acrosome reaction.; SUBCELLULAR LOCATION: [Isoform 2]: Secreted " Q9DA79,"PROTEIN NAMES: Dipeptidase 3 (Membrane-bound dipeptidase 3) (MBD-3) (Protein expressed in male leptotene and zygotene spermatocytes 136) (MLZ-136) PROTEIN FAMILY: Metallo-dependent hydrolases superfamily, Peptidase M19 family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the metallo-dependent hydrolases superfamily. Peptidase M19 family. FUNCTION: Lacks dipeptidase activity and is unable to hydrolyze cystinyl-bis-glycine. The absence of activity may be due to the inability of serine (instead of aspartate found in DPEP1/2) at position 356 to function as the acid/base catalyst and activate the nucleophilic water/hydroxide. Does not hydrolyze leukotriene D4 (LTD4) into leukotriene E4 (LTE4). Does not hydrolyze the beta-lactam antibiotic imipenem (By similarity). KEYWORDS: Disulfide bond;Glycoprotein;GPI-anchor;Lipoprotein;Membrane;Reference proteome;Signal SUBCELLULAR LOCATION: Membrane ; Lipid-anchor, GPI-anchor " Q9DAA4,"PROTEIN NAMES: Probable inactive serine protease 37 (Probable inactive trypsin-X2) PROTEIN FAMILY: Peptidase S1 family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the peptidase S1 family. FUNCTION: Plays a role in male fertility. May have a role in sperm migration or binding to zona-intact eggs. Involved in the activation of the proacrosin/acrosin system (By similarity). KEYWORDS: Cytoplasmic vesicle;Disulfide bond;Fertilization;Metal-binding;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, acrosome Secreted " Q9DAG6,"PROTEIN NAMES: GLIPR1-like protein 1 PROTEIN FAMILY: CRISP family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the CRISP family. FUNCTION: Required for optimal fertilization at the stage of sperm-oocyte fusion, plays a role in optimizing acrosome function, the translocation of IZUMO1 during the acrosome reaction and the fertilization process. Component of epididymosomes, one type of membranous microvesicules which mediate the transfer of lipids and proteins to spermatozoa plasma membrane during epididymal maturation. Also component of the CD9-positive microvesicules found in the cauda region. KEYWORDS: Cell membrane;Cytoplasmic vesicle;Fertilization;Glycoprotein;Membrane;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, acrosome Cell membrane ; Peripheral membrane protein ; Extracellular side Membrane raft Secreted Note=Located in the connecting piece of elongated spermatids and sperm. Also located in the apical region of the sperm head after sperm capacitation. Weakly attached to the cell membrane and later secreted into the extracellular space. Located on sperm equatorial segment and neck (By similarity). Associated with epididymosomes from the caput and cauda epididymis (By similarity). " Q9DBR4,"PROTEIN NAMES: Amyloid beta precursor protein binding family B member 2 (Amyloid-beta (A4) precursor protein-binding family B member 2) (Protein Fe65-like 1) ORGANISM: Mus musculus (Mouse) FUNCTION: Plays a role in the maintenance of lens transparency, and may also play a role in muscle cell strength. Involved in hippocampal neurite branching and neuromuscular junction formation, as a result plays a role in spatial memory functioning. Activates transcription of APP (By similarity). KEYWORDS: 3D-structure;Alternative splicing;Endoplasmic reticulum;Endosome;Golgi apparatus;Phosphoprotein;Reference proteome;Repeat SUBCELLULAR LOCATION: Endoplasmic reticulum Golgi apparatus Early endosome " Q9DBY0,PROTEIN NAMES: Forkhead box protein P4 (Fork head-related protein-like A) (mFKHLA) ORGANISM: Mus musculus (Mouse) FUNCTION: Transcriptional repressor that represses lung-specific expression. KEYWORDS: Alternative splicing;DNA-binding;Isopeptide bond;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Repressor;Transcription;Transcription regulation;Ubl conjugation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Q9DDE3,PROTEIN NAMES: Acetylcholinesterase (AChE) PROTEIN FAMILY: Type-B carboxylesterase/lipase family ORGANISM: Danio rerio (Zebrafish) (Brachydanio rerio) SIMILARITY: Belongs to the type-B carboxylesterase/lipase family. FUNCTION: Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. KEYWORDS: Cell membrane;Disulfide bond;Glycoprotein;Hydrolase;Membrane;Neurotransmitter degradation;Reference proteome;Secreted;Serine esterase;Signal;Synapse SUBCELLULAR LOCATION: Synapse. Secreted. Cell membrane ; Peripheral membrane protein MISCELLANEOUS: No other isoforms exist. This protein corresponds to the T isoform in other species. Q9EPK8,"PROTEIN NAMES: Transient receptor potential cation channel subfamily V member 4 (TrpV4) (Osm-9-like TRP channel 4) (OTRPC4) (Transient receptor potential protein 12) (TRP12) (Vanilloid receptor-like channel 2) (Vanilloid receptor-like protein 2) (Vanilloid receptor-related osmotically-activated channel) (VR-OAC) PROTEIN FAMILY: Transient receptor family, TrpV subfamily, TRPV4 sub-subfamily ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV4 sub-subfamily. FUNCTION: Non-selective calcium permeant cation channel involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. Also activated by heat, low pH, citrate and phorbol esters. Increase of intracellular Ca(2+) potentiates currents. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism (By similarity). Acts as a regulator of intracellular Ca(2+) in synoviocytes (By similarity). Plays an obligatory role as a molecular component in the nonselective cation channel activation induced by 4-alpha-phorbol 12,13-didecanoate and hypotonic stimulation in synoviocytes and also regulates production of IL-8 (By similarity). Together with PKD2, forms mechano- and thermosensitive channels in cilium. Promotes cell-cell junction formation in skin keratinocytes and plays an important role in the formation and/or maintenance of functional intercellular barriers. Negatively regulates expression of PPARGC1A, UCP1, oxidative metabolism and respiration in adipocytes. Regulates expression of chemokines and cytokines related to pro-inflammatory pathway in adipocytes. Together with AQP5, controls regulatory volume decrease in salivary epithelial cells. Required for normal development and maintenance of bone and cartilage (By similarity). In its inactive state, may sequester DDX3X at the plasma membrane. When activated, the interaction between both proteins is affected and DDX3X relocalizes to the nucleus (By similarity). In neurons of the central nervous system, could play a role in triggering voluntary water intake in response to increased sodium concentration in body fluid. KEYWORDS: 3D-structure;ANK repeat;ATP-binding;Calcium;Calcium channel;Calcium transport;Calmodulin-binding;Cell junction;Cell membrane;Cell projection;Cilium;Glycoprotein;Ion channel;Ion transport;Lipid-binding;Membrane;Metal-binding;Nucleotide-binding;Phosphoprotein;Reference proteome;Repeat;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane Apical cell membrane ; Multi-pass membrane protein Cell junction, adherens junction Cell projection, cilium Note=Assembly of the putative homotetramer occurs primarily in the endoplasmic reticulum. Localization to the cell membrane is inhibited by WNK kinases (WNK1, WNK2, WNK3 or WNK4) in a kinase-independent mechanism. " Q9EQ32,"PROTEIN NAMES: Phosphoinositide 3-kinase adapter protein 1 (B-cell adapter for phosphoinositide 3-kinase) (B-cell phosphoinositide 3-kinase adapter protein 1) ORGANISM: Mus musculus (Mouse) FUNCTION: Signaling adapter that contributes to B-cell development by linking B-cell receptor (BCR) signaling to the phosphoinositide 3-kinase (PI3K)-Akt signaling pathway. Has a complementary role to the BCR coreceptor CD19, coupling BCR and PI3K activation by providing a docking site for the PI3K subunit PIK3R1. Alternatively, links Toll-like receptor (TLR) signaling to PI3K activation, a process preventing excessive inflammatory cytokine production. Also involved in the activation of PI3K in natural killer cells. May be involved in the survival of mature B-cells via activation of REL. KEYWORDS: Alternative splicing;Cell membrane;Cytoplasm;Membrane;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Cell membrane ; Peripheral membrane protein " Q9EQJ0,"PROTEIN NAMES: Two pore calcium channel protein 1 (Voltage-dependent calcium channel protein TPC1) PROTEIN FAMILY: Calcium channel alpha-1 subunit family, Two pore calcium channel subfamily ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. Two pore calcium channel subfamily. FUNCTION: Intracellular channel initially characterized as a non-selective Ca(2+)-permeable channel activated by NAADP (nicotinic acid adenine dinucleotide phosphate), it is also a voltage-gated highly-selective Na(+) channel activated directly by PI(3,5)P2 (phosphatidylinositol 3,5-bisphosphate) that senses pH changes and confers electrical excitability to organelles. Localizes to the early and recycling endosomes membranes where it plays a role in the uptake and processing of proteins and regulates organellar membrane excitability, membrane trafficking and pH homeostasis. Ion selectivity is not fixed but rather agonist-dependent and under defined ionic conditions, can be readily activated by both NAADP and PI(3,5)P2 (By similarity). Required for mTOR-dependent nutrient sensing. KEYWORDS: 3D-structure;Alternative splicing;Calcium;Calcium channel;Calcium transport;Coiled coil;Endosome;Glycoprotein;Ion channel;Ion transport;Lysosome;Membrane;Reference proteome;Repeat;Transmembrane;Transmembrane helix;Transport;Voltage-gated channel SUBCELLULAR LOCATION: Lysosome membrane ; Multi-pass membrane protein Endosome membrane ; Multi-pass membrane protein Early endosome membrane ; Multi-pass membrane protein Recycling endosome membrane ; Multi-pass membrane protein " Q9EQN9,PROTEIN NAMES: Thiamine transporter 1 (ThTr-1) (Solute carrier family 19 member 2) PROTEIN FAMILY: Reduced folate carrier (RFC) transporter family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the reduced folate carrier (RFC) transporter (TC 2.A.48) family. FUNCTION: High-affinity transporter for the intake of thiamine. Essential for spermatogenesis. Mediates H(+)-dependent pyridoxine transport. KEYWORDS: Acetylation;Alternative splicing;Cell membrane;Differentiation;Glycoprotein;Membrane;Phosphoprotein;Reference proteome;Spermatogenesis;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Q9ER62,PROTEIN NAMES: Tumor necrosis factor receptor superfamily member 22 (Decoy TRAIL receptor 2) (TNF receptor family member SOBa) (TNF receptor homolog 2) (Tumor necrosis factor receptor p60 homolog 2) ORGANISM: Mus musculus (Mouse) FUNCTION: Receptor for the cytotoxic ligand TNFSF10/TRAIL. Lacks a cytoplasmic death domain and hence is not capable of inducing apoptosis. Protects cells against TRAIL mediated apoptosis possibly through ligand competition. Cannot induce the NF-kappa-B pathway. KEYWORDS: Alternative splicing;Cell membrane;Disulfide bond;Glycoprotein;Membrane;Receptor;Reference proteome;Repeat;Secreted;Signal-anchor;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: [Isoform 1]: Cell membrane ; Single-pass type II membrane protein.; SUBCELLULAR LOCATION: [Isoform 2]: Secreted Q9ERQ6,"PROTEIN NAMES: Chondroitin sulfate proteoglycan 5 (Acidic leucine-rich EGF-like domain-containing brain protein) (Neuroglycan C) ORGANISM: Rattus norvegicus (Rat) FUNCTION: May function as a growth and differentiation factor involved in neuritogenesis. May induce ERBB3 activation. KEYWORDS: Alternative splicing;Cell membrane;Developmental protein;Differentiation;Direct protein sequencing;Disulfide bond;EGF-like domain;Endoplasmic reticulum;Glycoprotein;Golgi apparatus;Growth regulation;Membrane;Neurogenesis;Phosphoprotein;Proteoglycan;Reference proteome;Secreted;Signal;Synapse;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein Synaptic cell membrane ; Single-pass type I membrane protein Endoplasmic reticulum membrane ; Single-pass type I membrane protein Golgi apparatus membrane ; Single-pass type I membrane protein Cell surface Secreted Note=In neurons, localizes to synaptic junctions. Also detected in the endoplasmic reticulum and the Golgi (By similarity). Partially enriched in lipid rafts. MISCELLANEOUS: Different forms of various molecular weight have been observed. Such forms are possibly due to different levels of glycosylation, phosphorylation and/or protein cleavage (By similarity)." Q9ERV7,"PROTEIN NAMES: p53-induced death domain-containing protein 1 (Leucine-rich repeat and death domain-containing protein) [Cleaved into: PIDD-N; PIDD-C; PIDD-CC] ORGANISM: Mus musculus (Mouse) FUNCTION: Component of the DNA damage/stress response pathway that functions downstream of p53/TP53 and can either promote cell survival or apoptosis. Associated with CRADD and the CASP2 caspase, it forms the PIDDosome a complex that activates CASP2 and triggers apoptosis. Associated with IKBKG and RIPK1, it enhances sumoylation and ubiquitination of IKBKG which is important for activation of the transcription factor NF-kappa-B (By similarity). KEYWORDS: Acetylation;Apoptosis;Cytoplasm;Hydrolase;Leucine-rich repeat;Nucleus;Phosphoprotein;Reference proteome;Repeat SUBCELLULAR LOCATION: Cytoplasm Nucleus Note=Enriched in the nucleus upon DNA damage. " Q9ES64,"PROTEIN NAMES: Harmonin (PDZ domain-containing protein) (Usher syndrome type-1C protein homolog) ORGANISM: Mus musculus (Mouse) FUNCTION: Anchoring/scaffolding protein that is a part of the functional network formed by USH1C, USH1G, CDH23 and MYO7A that mediates mechanotransduction in cochlear hair cells. Required for normal development and maintenance of cochlear hair cell bundles. As part of the intermicrovillar adhesion complex/IMAC plays a role in brush border differentiation, controlling microvilli organization and length. Probably plays a central regulatory role in the assembly of the complex, recruiting CDHR2, CDHR5 and MYO7B to the microvilli tips. KEYWORDS: 3D-structure;Alternative splicing;Cell projection;Coiled coil;Cytoplasm;Cytoskeleton;Differentiation;Hearing;Phosphoprotein;Reference proteome;Repeat SUBCELLULAR LOCATION: Cytoplasm, cytosol Cytoplasm, cytoskeleton Cell projection, microvillus Note=Colocalizes with F-actin. Detected at the tip of cochlear hair cell stereocilia. Enriched in microvilli of the intestinal brush border. " Q9ESE2,"PROTEIN NAMES: Tumor necrosis factor ligand superfamily member 11 (Osteoclast differentiation factor) (ODF) (Osteoprotegerin ligand) (OPGL) (Receptor activator of nuclear factor kappa-B ligand) (RANKL) (TNF-related activation-induced cytokine) (TRANCE) (CD antigen CD254) [Cleaved into: Tumor necrosis factor ligand superfamily member 11, membrane form; Tumor necrosis factor ligand superfamily member 11, soluble form] PROTEIN FAMILY: Tumor necrosis factor family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the tumor necrosis factor family. FUNCTION: Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy. Induces osteoclastogenesis by activating multiple signaling pathways in osteoclast precursor cells, chief among which is induction of long lasting oscillations in the intracellular concentration of Ca (2+) resulting in the activation of NFATC1, which translocates to the nucleus and induces osteoclast-specific gene transcription to allow differentiation of osteoclasts. During osteoclast differentiation, in a TMEM64 and ATP2A2-dependent manner induces activation of CREB1 and mitochondrial ROS generation necessary for proper osteoclast generation. KEYWORDS: Cell membrane;Cytokine;Developmental protein;Differentiation;Glycoprotein;Membrane;Receptor;Reference proteome;Secreted;Signal-anchor;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type II membrane protein.; SUBCELLULAR LOCATION: [Tumor necrosis factor ligand superfamily member 11, soluble form]: Secreted " Q9ESL4,"PROTEIN NAMES: Mitogen-activated protein kinase kinase kinase 20 (Leucine zipper- and sterile alpha motif kinase ZAK) (Leucine zipper- and sterile alpha motif-containing kinase) (MLK-like mitogen-activated protein triple kinase) (Mitogen-activated protein kinase kinase kinase MLT) (Mixed lineage kinase-related kinase) (MLK-related kinase) (MRK) (Sterile alpha motif- and leucine zipper-containing kinase AZK) PROTEIN FAMILY: Protein kinase superfamily, STE Ser/Thr protein kinase family, MAP kinase kinase kinase subfamily ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily. FUNCTION: Stress-activated component of a protein kinase signal transduction cascade that promotes programmed cell death in response to various stress, such as ribosomal stress, osmotic shock and ionizing radiation. Acts by catalyzing phosphorylation of MAP kinase kinases, leading to activation of the JNK (MAPK8/JNK1, MAPK9/JNK2 and/or MAPK10/JNK3) and MAP kinase p38 (MAPK11, MAPK12, MAPK13 and/or MAPK14) pathways. Activates JNK through phosphorylation of MAP2K4/MKK4 and MAP2K7/MKK7, and MAP kinase p38 gamma (MAPK12) via phosphorylation of MAP2K3/MKK3 and MAP2K6/MKK6. Involved in stress associated with adrenergic stimulation: contributes to cardiac decompensation during periods of acute cardiac stress (By similarity). May be involved in regulation of S and G2 cell cycle checkpoint by mediating phosphorylation of CHEK2 (By similarity).; FUNCTION: [Isoform ZAKalpha]: Key component of the stress-activated protein kinase signaling cascade in response to ribotoxic stress or UV-B irradiation. Acts as the proximal sensor of ribosome collisions during the ribotoxic stress response (RSR). Directly binds to the ribosome by inserting its flexible C-terminus into the ribosomal intersubunit space, thereby acting as a sentinel for colliding ribosomes (By similarity). Upon ribosome collisions, activates either the stress-activated protein kinase signal transduction cascade or the integrated stress response (ISR), leading to programmed cell death or cell survival, respectively (By similarity). Dangerous levels of ribosome collisions trigger the autophosphorylation and activation of MAP3K20, which dissociates from colliding ribosomes and phosphorylates MAP kinase kinases, leading to activation of the JNK and MAP kinase p38 pathways that promote programmed cell death (By similarity). Less dangerous levels of ribosome collisions trigger the integrated stress response (ISR): MAP3K20 activates EIF2AK4/GCN2 independently of its protein-kinase activity, promoting EIF2AK4/GCN2-mediated phosphorylation of EIF2S1/eIF-2-alpha (By similarity). Also acts as a histone kinase by phosphorylating histone H3 at 'Ser-28' (H3S28ph) (By similarity).; FUNCTION: [Isoform ZAKbeta]: Isoform that lacks the C-terminal region that mediates ribosome-binding: does not act as a sensor of ribosome collisions in response to ribotoxic stress. May act as an antagonist of isoform ZAKalpha: interacts with isoform ZAKalpha, leading to decrease the expression of isoform ZAKalpha. KEYWORDS: Acetylation;Alternative splicing;ATP-binding;Cell cycle;Cytoplasm;Direct protein sequencing;Kinase;Magnesium;Metal-binding;Nucleotide-binding;Nucleus;Phosphoprotein;Reference proteome;RNA-binding;rRNA-binding;Serine/threonine-protein kinase;Transferase SUBCELLULAR LOCATION: Cytoplasm Nucleus Note=Appears to shuttle between nucleus and cytoplasm. " Q9ET22,PROTEIN NAMES: Dipeptidyl peptidase 2 (Dipeptidyl aminopeptidase II) (Dipeptidyl peptidase 7) (Dipeptidyl peptidase II) (DPP II) (Quiescent cell proline dipeptidase) PROTEIN FAMILY: Peptidase S28 family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the peptidase S28 family. FUNCTION: Plays an important role in the degradation of some oligopeptides. KEYWORDS: Aminopeptidase;Cytoplasmic vesicle;Glycoprotein;Hydrolase;Lysosome;Protease;Reference proteome;Secreted;Serine protease;Signal;Zymogen SUBCELLULAR LOCATION: Lysosome Cytoplasmic vesicle Secreted Q9FEQ8,"PROTEIN NAMES: Peroxidase 2 (Plasma membrane-bound peroxidase 2) (pmPOX2) PROTEIN FAMILY: Peroxidase family, Classical plant (class III) peroxidase subfamily ORGANISM: Zea mays (Maize) SIMILARITY: Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily. FUNCTION: Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue. KEYWORDS: Calcium;Disulfide bond;Glycoprotein;Heme;Hydrogen peroxide;Iron;Metal-binding;Oxidoreductase;Peroxidase;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted " Q9FF81,"PROTEIN NAMES: Vacuolar protein sorting-associated protein 36 (AtVPS36) (ESCRT-II complex subunit VPS36) PROTEIN FAMILY: VPS36 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the VPS36 family. FUNCTION: Component of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex (By similarity). KEYWORDS: Coiled coil;Endosome;Protein transport;Reference proteome;Transport SUBCELLULAR LOCATION: Endosome " Q9FFH0,"PROTEIN NAMES: Transcription activator GLK2 (GBF'S PRO-RICH REGION-INTERACTING factor 2) (Golden2-like protein 2) (AtGLK2) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Transcriptional activator that functions with GLK1 to promote chloroplast development. Acts as an activator of nuclear photosynthetic genes involved in chlorophyll biosynthesis, light harvesting, and electron transport. Acts in a cell-autonomous manner to coordinate and maintain the photosynthetic apparatus within individual cells. May function in photosynthetic capacity optimization by integrating responses to variable environmental and endogenous cues. Prevents premature senescence. KEYWORDS: DNA-binding;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: Plants overexpressing GLK2 have a delay in flowering under long days." Q9FFZ1,"PROTEIN NAMES: Pheophytinase, chloroplastic (Pheophytin pheophorbide hydrolase) (Protein CO-REGULATED WITH NYE1) PROTEIN FAMILY: AB hydrolase superfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the AB hydrolase superfamily. FUNCTION: Alpha/beta hydrolase dephytylating specifically the Mg-free chlorophyll pigment (pheophytin), yielding pheophorbide. No activity on chlorophyll. Belongs to the chlorophyll catabolic enzymes (CCEs). KEYWORDS: Chlorophyll catabolism;Chloroplast;Hydrolase;Membrane;Plastid;Reference proteome;Thylakoid;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane. Plastid, chloroplast stroma. " Q9FG01,PROTEIN NAMES: Splicing factor SF3a60 homolog (Protein ATROPOS) (Splicing factor ATO) PROTEIN FAMILY: SF3A3 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the SF3A3 family. FUNCTION: Splicing factor homolog to SF3a60 that may be involved in pre-spliceosome formation. Is necessary for gametic cell fate determination. KEYWORDS: Acetylation;Metal-binding;mRNA processing;mRNA splicing;Nucleus;Phosphoprotein;Reference proteome;Spliceosome;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Q9FG38,"PROTEIN NAMES: Sorting nexin 1 (AtSNX1) (Vacuolar protein sorting-associated protein 5 homolog) PROTEIN FAMILY: Sorting nexin family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the sorting nexin family. FUNCTION: Plays a role in vesicular protein sorting. Acts at the crossroads between the secretory and endocytic pathways. Is involved in the endosome to vacuole protein transport via its interaction with the BLOS1/2 proteins and, as component of the membrane-associated retromer complex, is also involved in endosome-to-Golgi retrograde transport. Required for the auxin-carrier protein PIN2 sorting to the lytic vacuolar pathway and the trafficking of several plasma membrane proteins. Also involved in the efficient sorting of seed storage protein globulin 12S. KEYWORDS: Cytoplasm;Endosome;Golgi apparatus;Lipid-binding;Membrane;Phosphoprotein;Protein transport;Reference proteome;Transport SUBCELLULAR LOCATION: Cytoplasm. Endosome membrane; Peripheral membrane protein; Cytoplasmic side. Prevacuolar compartment membrane; Peripheral membrane protein; Cytoplasmic side. Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein; Cytoplasmic side. " Q9FG77,"PROTEIN NAMES: Probable WRKY transcription factor 2 (WRKY DNA-binding protein 2) PROTEIN FAMILY: WRKY group I family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the WRKY group I family. FUNCTION: Transcription factor. Regulates WOX8 and WOX9 expression and basal cell division patterns during early embryogenesis. Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element. Required to repolarize the zygote from a transient symmetric state. KEYWORDS: 3D-structure;Developmental protein;DNA-binding;Metal-binding;Nucleus;Reference proteome;Repeat;Transcription;Transcription regulation;Zinc SUBCELLULAR LOCATION: Nucleus " Q9FGI6,"PROTEIN NAMES: NADH dehydrogenase [ubiquinone] iron-sulfur protein 1, mitochondrial (Protein EMBRYO DEFECTIVE 1467) PROTEIN FAMILY: Complex I 75 kDa subunit family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the complex I 75 kDa subunit family. FUNCTION: Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized (By similarity). KEYWORDS: 2Fe-2S;3D-structure;4Fe-4S;Alternative splicing;Direct protein sequencing;Electron transport;Iron;Iron-sulfur;Membrane;Metal-binding;Mitochondrion;Mitochondrion inner membrane;NAD;Oxidoreductase;Reference proteome;Respiratory chain;Transit peptide;Translocase;Transport;Ubiquinone SUBCELLULAR LOCATION: Mitochondrion inner membrane Note=Matrix and cytoplasmic side of the mitochondrial inner membrane. " Q9FGN0,"PROTEIN NAMES: Conserved oligomeric Golgi complex subunit 7 (COG complex subunit 7) (Component of oligomeric Golgi complex 7) (Protein EMBRYO YELLOW) PROTEIN FAMILY: COG7 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the COG7 family. FUNCTION: Required for normal Golgi function. Necessary for embryo development and pigmentation, especially for the expansion of cells and organs, and for the formation of the organized shoot apical meristem (SAM). Probably involved in the generation of the extra-cellular matrix. KEYWORDS: Coiled coil;Golgi apparatus;Membrane;Protein transport;Reference proteome;Transport SUBCELLULAR LOCATION: Golgi apparatus membrane ; Peripheral membrane protein " Q9FGQ6,"PROTEIN NAMES: Microtubule-associated protein RP/EB family member 1C (APC-binding protein EB1C) (End-binding protein 1C) (AtEB1C) (Protein ATEB1 homolog 1) (AtEB1H1) PROTEIN FAMILY: MAPRE family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the MAPRE family. FUNCTION: Plant-specific EB1 subtype that functions preferentially at early stages of plant mitosis by regulating spindle positioning and chromosome segregation. Accumulates in the prophase nucleus and is required to maintain spindle bipolarity during premetaphase and/or metaphase and for efficient segregation of chromosomes at anaphase. May play a role in the dynamics of microtubule network in elongating pollen tubes. KEYWORDS: Cell cycle;Cell division;Cytoplasm;Cytoskeleton;Microtubule;Mitosis;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus. Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, phragmoplast. Note=During mitosis, accumulates in the prophase nucleus, and after the nuclear envelope disintegration is associated with whole spindle microtubules, plus end of microtubules, phragmoplast and finally is actively recruited to the nucleus. Localizes in the microtubule network in elongating pollen tubes. MISCELLANEOUS: Plant microtubules behave differently from those of other eukaryotes in mitosis: they lack centrosomes and spindles are barrel-shaped with unfocused poles and no astral microtubules." Q9FGS5,PROTEIN NAMES: High-affinity nitrate transporter 3.1 (Protein WOUND-RESPONSIVE 3) PROTEIN FAMILY: NAR2 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the NAR2 family. FUNCTION: Acts as a dual component transporter with NTR2.1. Required for high-affinity nitrate transport. Acts as a repressor of lateral root initiation. May be involved in targeting NRT2 proteins to the plasma membrane. KEYWORDS: Cell membrane;Membrane;Nitrate assimilation;Reference proteome;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass membrane protein Q9FGT1,"PROTEIN NAMES: Protein REPRESSOR OF SILENCING 3 ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: RNA-binding protein required for DNA demethylation and to eluviate siRNA-mediated transcriptional gene silencing (TGS), probably by guiding ROS1. Can bind specifically single stranded G-rich RNAs of 21-, 24- or 26-nt corresponding to promoter sequence of target genes; this interaction directs demethylation of target sequences. KEYWORDS: Nucleus;Reference proteome;RNA-binding;RNA-mediated gene silencing SUBCELLULAR LOCATION: Nucleus Nucleus, nucleolus Nucleus, nucleoplasm Note=Localized in discrete foci dispersed throughout the nucleus. " Q9FH36,"PROTEIN NAMES: Probable galacturonosyltransferase 12 (Like glycosyl transferase 6) (Protein IRREGULAR XYLEM 8) PROTEIN FAMILY: Glycosyltransferase 8 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the glycosyltransferase 8 family. FUNCTION: Involved in pectin assembly and/or distribution, and in the synthesis of secondary wall glucuronoxylan. Probably involved in the synthesis of the glycosyl sequence at the glucuronoxylan reducing end. May be involved in synthesis of a complex glycan primer for xylan synthesis. PATHWAY: Glycan metabolism; pectin biosynthesis. KEYWORDS: Cell wall biogenesis/degradation;Glycoprotein;Glycosyltransferase;Golgi apparatus;Membrane;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus membrane ; Single-pass type II membrane protein " Q9FHM4,"PROTEIN NAMES: 65-kDa microtubule-associated protein 3 (AtMAP65-3) (Protein PLEIADE) PROTEIN FAMILY: MAP65/ASE1 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the MAP65/ASE1 family. FUNCTION: Microtubule-associated protein that plays a critical role in organizing the mitotic microtubule array during both early and late mitosis in all plant organs. Essential for the cytokinesis, especially in roots, by maintaining the integrity of the overlapped microtubules in the phragmoplast. Required during root morphogenesis. Needed for giant cell development during root knot nematode infection, where cytokinesis is initiated but not completed. KEYWORDS: Cell cycle;Cell division;Coiled coil;Cytoplasm;Cytoskeleton;Microtubule;Mitosis;Nucleus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Cytoplasm, cytoskeleton, phragmoplast. Note=Locates only to the mitotic MT arrays. Present in MT cortical arrays just before mitosis. Associates to MT in preprophase band, during anaphase, and in phragmoplast, including midzone. Distributed diffusely through the cytoplasm during metaphase only. At the end of cytokinesis, present only at the cell periphery, forming a ring around the newly formed cell plate. During root knot nematode infection, targeted to the giant cell mini cell plate. " Q9FIL7,"PROTEIN NAMES: Calmodulin-binding receptor-like cytoplasmic kinase 1 PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. KEYWORDS: ATP-binding;Cytoplasm;Kinase;Nucleotide-binding;Phosphoprotein;Reference proteome;Serine/threonine-protein kinase;Transferase SUBCELLULAR LOCATION: Cytoplasm " Q9FIU5,"PROTEIN NAMES: Calcium/calmodulin-regulated receptor-like kinase 1 (AtCRLK1) PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. FUNCTION: Required for cold tolerance, via the activation of MAP kinases activity. Phosphorylates and activates MEKK1 in response to cold in a calcium-dependent manner. KEYWORDS: Alternative splicing;ATP-binding;Calmodulin-binding;Cell membrane;Endosome;Kinase;Membrane;Nucleotide-binding;Phosphoprotein;Receptor;Reference proteome;Serine/threonine-protein kinase;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass membrane protein Endosome membrane ; Single-pass membrane protein " Q9FJG9,"PROTEIN NAMES: RCC1 domain-containing protein RUG3, mitochondrial (RCC1/UVR8/GEF-like protein 3, mitochondrial) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Regulates DNA damage response (DDR) synergistically with ATM. Together with ATM, involved in the splicing of the ND2/NAD2 mRNA. Required for the accumulation of mitochondrial respiratory chain complex I. Negative regulator of plant responses to abscisic acid (ABA). May have a pivotal role in vegetative growth and the phase transition from vegetative to reproductive growth. KEYWORDS: Abscisic acid signaling pathway;Alternative splicing;DNA damage;Mitochondrion;mRNA processing;mRNA splicing;Reference proteome;Repeat;Transit peptide SUBCELLULAR LOCATION: Mitochondrion " Q9FJR9,"PROTEIN NAMES: Nuclear intron maturase 2, mitochondrial (AtnMat2) (Nuclear intron maturase 1 b) (AtnMat1b) PROTEIN FAMILY: Plant nuclear intron maturase (nMat) family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the plant nuclear intron maturase (nMat) family. FUNCTION: Nuclear-encoded maturase required for splicing of group-II introns in mitochondria. Involved in the splicing of mitochondrial COX2, NAD1 and NAD7 transcripts. Necessary for mitochondrial biogenesis during early developmental stages. KEYWORDS: Endonuclease;Hydrolase;Intron homing;Mitochondrion;Nuclease;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Mitochondrion " Q9FK51,PROTEIN NAMES: ADP-glucose phosphorylase (ADP-glucose:phosphate adenylyltransferase) PROTEIN FAMILY: Galactose-1-phosphate uridylyltransferase type 1 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the galactose-1-phosphate uridylyltransferase type 1 family. FUNCTION: Catalyzes the conversion of ADP-glucose and inorganic phosphate (Pi) into glucose-1-phosphate and ADP. Does not possess galactose-1-phosphate uridylyltransferase activity. KEYWORDS: 3D-structure;Carbohydrate metabolism;Glucose metabolism;Metal-binding;Nucleotidyltransferase;Reference proteome;Transferase;Zinc MISCELLANEOUS: Functions by a double-displacement chemical mechanism and ping-pong kinetics through a covalent nucleotidyl-enzyme intermediate. Q9FKL2,"PROTEIN NAMES: Transcription factor MYB36 (Myb-related protein 36) (AtMYB36) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Transcription factors that activates genes required for endodermal differentiation but represses genes involved in proliferative divisions, thus regulating the transition from proliferation to differentiation in root endodermis. Required for Casparian strip formation by positively regulating the expression of the Casparian strip genes CASP1, PER64 and ESB1 and other endodermis-specific genes, thus triggering correct localized lignin biosynthesis in root endodermis and subsequently regulating global ion homeostasis. KEYWORDS: Activator;DNA-binding;Nucleus;Reference proteome;Repeat;Repressor;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " Q9FLZ5,"PROTEIN NAMES: Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and protein-tyrosine-phosphatase PTEN1 (Protein PHOSPHATASE AND TENSIN HOMOLOG 1) (AtPTEN1) PROTEIN FAMILY: PTEN phosphatase protein family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the PTEN phosphatase protein family. FUNCTION: Protein tyrosine phosphatase that exhibits also lipid phosphatase activity. Can use phosphatidylinositol substrates such as PtdIns(3,4,5)P(3) as substrate. Pollen-specific phosphatase required for pollen development. KEYWORDS: Hydrolase;Lipid metabolism;Phospholipid metabolism;Protein phosphatase;Reference proteome " Q9FM03,"PROTEIN NAMES: Dof zinc finger protein DOF5.6 (AtDOF5.6) (Protein HIGH CAMBIAL ACTIVITY 2) (Protein PHLOEM EARLY DOF HCA2) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Transcription factor that binds specifically to a 5'-AA[AG]G-3' consensus core sequence (By similarity). Promotes expression. The PEAR proteins (e.g. DOF2.4, DOF5.1, DOF3.2, DOF1.1, DOF5.6 and DOF5.3) activate gene expression that promotes radial growth of protophloem sieve elements. Involved in the regulation of interfascicular cambium formation and vascular tissue development, particularly at a very early stage during inflorescence stem development; promotes both cambium activity and phloem specification, but prevents xylem specification. KEYWORDS: Activator;DNA-binding;Metal-binding;Nucleus;Reference proteome;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus " Q9FM79,"PROTEIN NAMES: Pectinesterase QRT1 (PE QRT1) (Pectin methylesterase 62) (AtPME62) (Pectin methylesterase QRT1) (Protein QUARTET 1) (AtQRT1) PROTEIN FAMILY: Pectinesterase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the pectinesterase family. FUNCTION: Pectinesterase required for cell type-specific pectin degradation to separate microspores. PATHWAY: Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5. KEYWORDS: Aspartyl esterase;Cell wall;Cell wall biogenesis/degradation;Disulfide bond;Glycoprotein;Hydrolase;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted, cell wall " Q9FME4,"PROTEIN NAMES: Small ribosomal subunit protein mL104 (rPPR9) (PPR PROTEIN LOCALIZED TO THE NUCLEUS AND MITOCHONDRIA 1) (Pentatricopeptide repeat-containing protein PNM1, mitochondrial) PROTEIN FAMILY: PPR family, P subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the PPR family. P subfamily. FUNCTION: RNA-binding protein that functions in both mitochondrion and nucleus. In mitochondrion, it is associated with polysomes and may play a role in translation. Required during embryogenesis. In nucleus, might be involved in the regulation of its own gene expression. KEYWORDS: 3D-structure;Mitochondrion;Nucleus;Reference proteome;Repeat;Ribonucleoprotein;Ribosomal protein;RNA-binding;Transcription;Transcription regulation;Transit peptide;Translation regulation SUBCELLULAR LOCATION: Mitochondrion matrix Nucleus Note=Can localize to both mitochondrion and nucleus. " Q9FMK7,PROTEIN NAMES: BTB/POZ and TAZ domain-containing protein 1 (BTB and TAZ domain protein 1) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: May act as a substrate-specific adapter of an E3 ubiquitin-protein ligase complex (CUL3-RBX1-BTB) which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Also targeted for degradation by the 26S proteasome pathway. May be involved in gametophyte development. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Cytoplasm;Metal-binding;Nucleus;Reference proteome;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Q9FMN2,"PROTEIN NAMES: Phosphatidate phosphatase PAH2 (Phosphatidic acid phosphohydrolase 2) (AtPAH2) PROTEIN FAMILY: Lipin family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the lipin family. FUNCTION: Magnesium-dependent phosphatidate phosphatase which catalyzes the dephosphorylation of phosphatidate to yield diacylglycerol. Acts redundantly with PAH1 to repress phospholipid biosynthesis at the endoplasmic reticulum (ER). May function indirectly as repressor of multiple enzymes involved in phospholipid biosynthesis. Is involved in the pathway of galactolipid synthesis in the ER, which is required for the membrane lipid remodeling, an essential adaptation mechanism to cope with phosphate starvation. KEYWORDS: Alternative splicing;Cytoplasm;Hydrolase;Lipid biosynthesis;Lipid metabolism;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cytoplasm, cytosol. " Q9FMP3,"PROTEIN NAMES: Dihydropyrimidinase (Dihydropyrimidine amidohydrolase) (Protein PYRIMIDINE 2) PROTEIN FAMILY: Metallo-dependent hydrolases superfamily, Hydantoinase/dihydropyrimidinase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the metallo-dependent hydrolases superfamily. Hydantoinase/dihydropyrimidinase family. FUNCTION: Catalyzes the second step of the reductive pyrimidine degradation, the reversible hydrolytic ring opening of dihydropyrimidines. Can catalyze the ring opening of 5,6-dihydrouracil to N-carbamoyl-alanine and of 5,6-dihydrothymine to N-carbamoyl-amino isobutyrate. Involved in the recycling of nitrogen from nucleobases to general nitrogen metabolism. PATHWAY: Amino-acid biosynthesis; beta-alanine biosynthesis. KEYWORDS: Endoplasmic reticulum;Hydrolase;Metal-binding;Reference proteome;Zinc SUBCELLULAR LOCATION: Endoplasmic reticulum " Q9FMT1,"PROTEIN NAMES: 3-isopropylmalate dehydrogenase 1, chloroplastic (3-IPM-DH 1) (AtIMDH1) (IMDH 1) (Beta-IPM dehydrogenase 1) (Isopropylmalate dehydrogenase 1) (AtIMD1) (Methylthioalkylmalate dehydrogenase 1) PROTEIN FAMILY: Isocitrate and isopropylmalate dehydrogenases family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the isocitrate and isopropylmalate dehydrogenases family. FUNCTION: Involved in both glucosinolate and leucine biosynthesis; catalyzes the oxidative decarboxylation step in both leucine biosynthesis (primary metabolism) and methionine chain elongation of glucosinolates (specialized metabolism). Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate, 3-IPM) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate. Required during pollen development and involved in embryo sac development. More active on 3-isopropylmalate and NAD(+) than towards D-malate. PATHWAY: Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 3/4.; PATHWAY: Secondary metabolite biosynthesis. KEYWORDS: Alternative splicing;Amino-acid biosynthesis;Branched-chain amino acid biosynthesis;Chloroplast;Leucine biosynthesis;Magnesium;Manganese;Metal-binding;NAD;Oxidoreductase;Phosphoprotein;Plastid;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast stroma " Q9FMT4,PROTEIN NAMES: SWI/SNF complex component SNF12 homolog PROTEIN FAMILY: SMARCD family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the SMARCD family. FUNCTION: Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). KEYWORDS: 3D-structure;Chromatin regulator;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus Q9FMY3,"PROTEIN NAMES: Serine/threonine-protein kinase-like protein At5g23170 PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. KEYWORDS: ATP-binding;Coiled coil;Kinase;Nucleotide-binding;Reference proteome;Serine/threonine-protein kinase;Transferase MISCELLANEOUS: This gene is linked with a growth rate QTL (quantitative trait locus)." Q9FNQ0,PROTEIN NAMES: Protein LEO1 homolog (Protein VERNALIZATION INDEPENDENCE 4) PROTEIN FAMILY: LEO1 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the LEO1 family. FUNCTION: Component of the PAF1 complex (PAF1C) which is involved in histone modifications such as methylation on histone H3 'Lys-4' (H3K4me3). Involved in regulation of flowering time. Required for the expression of the flowering repressor and MADS box gene FLC. Involved in the control of seed dormancy and germination. KEYWORDS: Alternative splicing;Coiled coil;Flowering;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus Q9FNR3,"PROTEIN NAMES: Exocyst complex component EXO70E2 (AtExo70e2) (Exocyst subunit Exo70 family protein E2) PROTEIN FAMILY: EXO70 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the EXO70 family. FUNCTION: Influences the subcellular localization patterns of other exocyst complex proteins (e.g. SEC5A, SEC15A, SEC15B and EXO84B) leading to their recruitment to exocyst, well-defined large punctate structures throughout the cytosol. Essential component for the formation and the recruitment of exocyst subunits to the exocyst-positive organelle (EXPO), a secreted double membrane structure also called extracellular exosome, that acts as a sequester for cytosolic proteins to release them into the apoplast. KEYWORDS: Cell membrane;Cytoplasm;Exocytosis;Membrane;Protein transport;Reference proteome;Secreted;Transport SUBCELLULAR LOCATION: Secreted, extracellular exosome Secreted Cell membrane Cytoplasm Endomembrane system Note=Localized to well-defined large punctate structures throughout the cytosol. Component of the secreted double membrane structure exocyst-positive organelle (EXPO). Locates to the plasma membrane as discrete punctae and secreted outside of the cells. " Q9FNX8,"PROTEIN NAMES: Lipoxygenase 4, chloroplastic (AtLOX4) (LOX3-like protein) PROTEIN FAMILY: Lipoxygenase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the lipoxygenase family. FUNCTION: Plant lipoxygenases may be involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence or responses to wounding. Catalyzes the hydroperoxidation of lipids containing a cis,cis-1,4-pentadiene structure (By similarity). 13S-lipoxygenase that can use linolenic acid as substrates. PATHWAY: Lipid metabolism; oxylipin biosynthesis. KEYWORDS: Chloroplast;Dioxygenase;Fatty acid biosynthesis;Fatty acid metabolism;Iron;Lipid biosynthesis;Lipid metabolism;Metal-binding;Oxidoreductase;Oxylipin biosynthesis;Plastid;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast " Q9FRL5,PROTEIN NAMES: Zinc-finger homeodomain protein 5 (AtZHD5) (Homeobox protein 33) (AtHB-33) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Putative transcription factor. Binds DNA at 5'-ATTA-3' consensus promoter regions. Regulates floral architecture and leaf development. Regulators in the abscisic acid (ABA) signal pathway that confers sensitivity to ABA in an ARF2-dependent manner. KEYWORDS: Abscisic acid signaling pathway;DNA-binding;Homeobox;Metal-binding;Nucleus;Reference proteome;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Note=Interactions with MIF proteins prevent nuclear subcellular location and leads to a scattered repartition throughout the cytoplasm. Q9FSH9,"PROTEIN NAMES: Gamma conglutin 1 (Conglutin gamma 32) (allergen Lup a gamma-conglutin) [Cleaved into: Gamma conglutin 1 beta subunit (Gamma conglutin 1 17 kDa subunit) (Gamma conglutin 1 small subunit); Gamma conglutin 1 alpha subunit (Gamma conglutin 1 29 kDa subunit) (Gamma conglutin 1 large subunit)] PROTEIN FAMILY: Peptidase A1 family ORGANISM: Lupinus albus (White lupine) (Lupinus termis) SIMILARITY: Belongs to the peptidase A1 family. FUNCTION: Sulfur-rich seed storage protein that remains undegraded at germination. The uncleaved form exhibits some inhibitory activity against GH11 xylanase from T.longibrachiatum, more at pH 7 than at pH 5.3, but not against GH12 xyloglucan-specific endoglucanase (XEG) from A.aculeatus. Binds to model phospholipid membranes containing dimyristoyl phosphatidylglycerol (DMPG), dioleoyl phosphatidic acid (DOPA) or mixture of dimyristoyl phosphatidylcholine and dimyristoyl phosphatidylglycerol (DMPC:DMPG), or mixture of dioleoyl phosphatidic acid and dioleoyl phosphatidylcholine (DOPC:DOPA). KEYWORDS: Allergen;Direct protein sequencing;Disulfide bond;Glycoprotein;Secreted;Signal SUBCELLULAR LOCATION: Secreted, extracellular space Note=Present in the extracellular spaces of germinating cotyledons and in the young roots. MISCELLANEOUS: Resistant to pancreatin-mediated digestion.; MISCELLANEOUS: Mediates a dose-dependent decrease of blood plasma glucose and increased Ins1 expression and pancreatic insulin levels in hyperglycaemic/diabetic rats daily fed with gamma conglutin 1 or supplemented pasta. Involved in reducing rat hepatic glucose production, mainly through G6pc inhibition in impaired glucose metabolism disorders, but without leading to hypoglycemia. Triggers an increased glucose consumption of human liver hepatocellular HepG2 cells and potentiates the activity of insulin and metformin in cell glucose consumption. Internalized by and subsequently phosphorylated at Thr-69, Thr-182, Thr-331, Tyr-354, Ser-360 and Ser-442 in HepG2 cells cytoplasm. Exhibits hypoglycemic effect when orally administered 30 minutes before carbohydrate supply in human trials, but insulin concentrations are not affected." Q9FT72,"PROTEIN NAMES: ATP-dependent DNA helicase Q-like 3 (DNA 3'-5' helicase RecQ3) (RecQ-like protein 3) (AtRecQ3) (AtRecQl3) PROTEIN FAMILY: Helicase family, RecQ subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the helicase family. RecQ subfamily. FUNCTION: 3'-5' DNA helicase that may play a role in the repair of DNA. Exhibits an ATP or dATP-dependent DNA-helicase activity. Cannot use GTP/dGTP, CTP/dCTP or UTP/dUTP as nucleotide cofactors. Catalyzes DNA strand annealing. On nicked Holliday junctions, unwinds the lagging strand. Cannot act on intact Holliday junctions. KEYWORDS: Alternative splicing;ATP-binding;Coiled coil;DNA-binding;Helicase;Hydrolase;Isomerase;Magnesium;Manganese;Metal-binding;Nucleotide-binding;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus " Q9FT77,"PROTEIN NAMES: Disease resistance protein RLM3 (Protein RESISTANCE TO LEPTOSPHAERIA MACULANS 3) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: TIR-NB-LRR receptor-like protein that confers resistance to the pathogens Leptosphaeria maculans (blackleg disease), Botrytis cinerea, Alternaria brassicicola and Alternaria brassicae. Required for efficient callose deposition downstream of RLM1 during infection with L.maculans. KEYWORDS: Alternative splicing;ATP-binding;Hydrolase;NAD;Nucleotide-binding;Plant defense;Reference proteome;Repeat " Q9FV70,"PROTEIN NAMES: Transcription factor E2FC (E2F transcription factor-2) (AtE2F2) PROTEIN FAMILY: E2F/DP family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the E2F/DP family. FUNCTION: Involved in transcriptional repression. May act by repressing E2F-regulated genes in mature differentiated cells, but is not an antagonist of E2FA. Restricts cell division and is involved in the coordination between cell proliferation and endoreduplication during development. May play a role during the transition from skotomorphogenesis to photomorphogenesis. Regulated by phosphorylation-dependent proteolysis via the protein-ubiquitin ligase SCF(SKP2A) complex. KEYWORDS: Alternative splicing;Cell cycle;Coiled coil;Cytoplasm;DNA-binding;Reference proteome;Repressor;Transcription;Transcription regulation SUBCELLULAR LOCATION: Cytoplasm Note=Interaction with either DPA or DPB are unable to induce a nuclear localization. " Q9FVI6,"PROTEIN NAMES: Homeobox-leucine zipper protein HDG6 (HD-ZIP protein HDG6) (Homeobox protein FWA) (Homeodomain GLABRA 2-like protein 6) (Homeodomain transcription factor HDG6) (Protein HOMEODOMAIN GLABROUS 6) PROTEIN FAMILY: HD-ZIP homeobox family, Class IV subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the HD-ZIP homeobox family. Class IV subfamily. FUNCTION: Probable transcription factor involved in the regulation of time of flowering through the photoperiod flowering pathway. May repress FT. KEYWORDS: Coiled coil;Developmental protein;DNA-binding;Homeobox;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: Under epigenetic control. In wild-type plant, FWA is silenced in the sporophyte. The epi-allele fwa mutants (which do not have a change in the nucleotide sequence of FWA) cause a late-flowering phenotype due to ectopic FWA expression in sporophytic tissues. Repression of FWA is dependent on histone H3 'Lys-4' methylation and cytosine methylation which are partly controlled by the lysine-specific demthylase 1 (LSD1) homologs, LDL1 and LDL2." Q9FX43,"PROTEIN NAMES: Mitogen-activated protein kinase kinase 9 (AtMKK9) (MAP kinase kinase 9) PROTEIN FAMILY: Protein kinase superfamily, STE Ser/Thr protein kinase family, MAP kinase kinase subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily. FUNCTION: MKK9-MPK3/MPK6 module phosphorylates and activates EIN3, leading to the promotion of EIN3-mediated transcription in ethylene signaling. Autophosphorylates and also phosphorylates MPK3 and MPK6. Plays an important role in ethylene and camalexin biosynthesis and in salt stress response. MKK9-MPK6 module positively regulates leaf senescence. KEYWORDS: ATP-binding;Cytoplasm;Ethylene biosynthesis;Kinase;Nucleotide-binding;Nucleus;Phosphoprotein;Reference proteome;Serine/threonine-protein kinase;Stress response;Transferase SUBCELLULAR LOCATION: Cytoplasm Nucleus Note=Translocated into the nucleus by ACC treatment. " Q9FYE1,"PROTEIN NAMES: Metacaspase-9 (AtMC9) [Cleaved into: Metacaspase-9 subunit p20; Metacaspase-9 subunit p10 (Metacaspase 2f) (AtMCP2f)] PROTEIN FAMILY: Peptidase C14B family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the peptidase C14B family. FUNCTION: Cysteine protease that cleaves specifically after arginine or lysine residues. Does not cleave caspase-specific substrates. Required for proteolytic processing of GRI. KEYWORDS: 3D-structure;Apoplast;Autocatalytic cleavage;Direct protein sequencing;Glycoprotein;Hydrolase;Protease;Reference proteome;S-nitrosylation;Secreted;Thiol protease SUBCELLULAR LOCATION: Secreted, extracellular space, apoplast " Q9FYS5,"PROTEIN NAMES: HMG-Y-related protein A (ZmHMGA) (High mobility group A protein) PROTEIN FAMILY: Histone H1/H5 family ORGANISM: Zea mays (Maize) SIMILARITY: Belongs to the histone H1/H5 family. FUNCTION: Binds A/T-rich DNA (e.g. present in the storage gamma-zein gene promoter) with a highly dynamic distribution into the nucleus. Probably involved in endosperm development, during cells shift from a mitotic cycle to endoreduplication leading to massive synthesis of storage proteins (zeins) and starch. KEYWORDS: Acetylation;DNA-binding;Nucleus;Phosphoprotein;Reference proteome;Repeat SUBCELLULAR LOCATION: Nucleus, nucleolus Note=Follows a highly dynamic speckled distribution pattern throughout the chromatin of interphase nuclei. MISCELLANEOUS: Confers resistance to nickel NiSO(4) when expressed in yeast (e.g. S.cerevisiae) by counterbalancing nickel impact on cell cycle associated with an increased chromatin condensation." Q9FZ33,PROTEIN NAMES: Protein AUXIN RESPONSE 4 ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Required for the auxin influx facilitator AUX1 polar trafficking and its asymmetric localization within the plasma membrane. Not involved in the PIN proteins localization. KEYWORDS: Endoplasmic reticulum;Membrane;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Single-pass membrane protein Q9FZ97,"PROTEIN NAMES: Probable hexosyltransferase MUCI70 (Protein MUCILAGE-RELATED 70) PROTEIN FAMILY: Glycosyltransferase 8 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the glycosyltransferase 8 family. FUNCTION: Probable glycosyltransferase involved in pectin and/or xylans biosynthesis in cell walls (By similarity). Together with IRX14, required for xylan and pectin synthesis in seed coat epidermal (SCE) cells. Collaboratively with GAUT11, essential for the accumulation of seed mucilage, a gelatinous wall rich in unbranched rhamnogalacturonan I (RG I), and for shaping the surface morphology of seeds. PATHWAY: Glycan metabolism; pectin biosynthesis. KEYWORDS: Glycoprotein;Golgi apparatus;Membrane;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus membrane ; Single-pass type II membrane protein " Q9FZA2,"PROTEIN NAMES: Non-classical arabinogalactan protein 31 (Hydroxyproline-rich arabinogalactan protein 31) PROTEIN FAMILY: Non-classical AGP family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the non-classical AGP family. FUNCTION: Proteoglycan that may contribute to the strengthening of cell walls. KEYWORDS: Alternative splicing;Cell wall;Glycoprotein;Hydroxylation;Proteoglycan;Reference proteome;Repeat;Secreted;Signal SUBCELLULAR LOCATION: Secreted, cell wall " Q9FZF1,"PROTEIN NAMES: Peroxisomal membrane protein 11A (Peroxin-11A) (AtPEX11a) PROTEIN FAMILY: Peroxin-11 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the peroxin-11 family. FUNCTION: Involved in peroxisomal proliferation. Promotes peroxisomal duplication, aggregation or elongation without fission. KEYWORDS: Membrane;Peroxisome;Peroxisome biogenesis;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Peroxisome membrane ; Multi-pass membrane protein " Q9GNK5,"PROTEIN NAMES: Peptidoglycan-recognition protein LC (Immune response deficient 7 protein) PROTEIN FAMILY: N-acetylmuramoyl-L-alanine amidase 2 family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family. FUNCTION: Major activator of the imd/Relish pathway and is likely to encode a pattern recognition molecule for the humoral immune response. Required for Relish processing and nuclear translocation following proteolytic cleavage. Involved in the response to lipopolysaccharide (LPS) and peptidoglycan of Gram-negative bacteria. The different isoforms probably display different recognition capabilities to various microbial patterns.; FUNCTION: [Isoform a]: Mediates the response to LPS and Gram-negative bacteria.; FUNCTION: [Isoform x]: Mediates the response to LPS, peptidoglycan and Gram-negative bacteria. KEYWORDS: 3D-structure;Alternative splicing;Disulfide bond;Glycoprotein;Immunity;Innate immunity;Membrane;Reference proteome;Signal-anchor;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Single-pass type II membrane protein " Q9GPS0,"PROTEIN NAMES: Rho-related protein racG PROTEIN FAMILY: Small GTPase superfamily, Rho family ORGANISM: Dictyostelium discoideum (Social amoeba) SIMILARITY: Belongs to the small GTPase superfamily. Rho family. KEYWORDS: Cell membrane;GTP-binding;Lipoprotein;Membrane;Methylation;Nucleotide-binding;Prenylation;Reference proteome SUBCELLULAR LOCATION: Cell membrane ; Lipid-anchor ; Cytoplasmic side " Q9GRT1,"PROTEIN NAMES: Putative mitogen-activated protein kinase kinase 4 (LmxPK4) PROTEIN FAMILY: Protein kinase superfamily, STE Ser/Thr protein kinase family, MAP kinase kinase subfamily ORGANISM: Leishmania mexicana SIMILARITY: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily. FUNCTION: Protein kinase which regulates promastigote proliferation in host macrophages, thereby playing an essential role in parasite virulence. KEYWORDS: ATP-binding;Cell projection;Cilium;Cytoplasm;Flagellum;Kinase;Magnesium;Metal-binding;Nucleotide-binding;Nucleus;Transferase SUBCELLULAR LOCATION: Cytoplasm, cytosol Cell projection, cilium, flagellum Nucleus " Q9GRU1,"PROTEIN NAMES: Mitogen-activated protein kinase 4 (LmxMPK4) PROTEIN FAMILY: Protein kinase superfamily, CMGC Ser/Thr protein kinase family, MAP kinase subfamily ORGANISM: Leishmania mexicana SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. FUNCTION: Essential for the two main proliferating life stages, the promastigotes and amastigotes, of the parasite. KEYWORDS: ATP-binding;Kinase;Magnesium;Metal-binding;Nucleotide-binding;Phosphoprotein;Serine/threonine-protein kinase;Transferase " Q9GRW0,"PROTEIN NAMES: Phenoloxidase-activating factor 2 (45 KDa PPAF) (Hd-45) (Prophenoloxidase-activating factor II) (Serine protease-like PPAF-2) [Cleaved into: Phenoloxidase-activating factor 2 light chain; Phenoloxidase-activating factor 2 heavy chain] PROTEIN FAMILY: Peptidase S1 family, CLIP subfamily ORGANISM: Holotrichia diomphalia (Korean black chafer) SIMILARITY: Belongs to the peptidase S1 family. CLIP subfamily. FUNCTION: Binds and activates processed prophenoloxidases PPO1 and PPO2 and thus is involved in the activation of the prophenoloxidase cascade probably following the recognition of pathogen-derived products. KEYWORDS: 3D-structure;Antibiotic;Antimicrobial;Calcium;Direct protein sequencing;Disulfide bond;Glycoprotein;Immunity;Innate immunity;Metal-binding;Secreted;Serine protease homolog;Signal SUBCELLULAR LOCATION: Secreted Note=Secreted in the hemolymph. MISCELLANEOUS: Stabilized by calcium." Q9GRX6,"PROTEIN NAMES: Apoptosis-inducing factor 1, mitochondrial (Ddaif) PROTEIN FAMILY: FAD-dependent oxidoreductase family ORGANISM: Dictyostelium discoideum (Social amoeba) SIMILARITY: Belongs to the FAD-dependent oxidoreductase family. FUNCTION: Probable NADH oxidoreductase that acts as a caspase-independent mitochondrial effector of apoptotic cell death. KEYWORDS: Apoptosis;Cytoplasm;FAD;Flavoprotein;Mitochondrion;NAD;Nucleus;Oxidoreductase;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Mitochondrion Cytoplasm Nucleus Note=Translocates from mitochondria to the cytoplasm and secondary to the nucleus after the onset of cell death. " Q9GS23,"PROTEIN NAMES: ATP synthase subunit alpha, mitochondrial (ATP synthase F1 subunit alpha) PROTEIN FAMILY: ATPase alpha/beta chains family ORGANISM: Trypanosoma brucei brucei SIMILARITY: Belongs to the ATPase alpha/beta chains family. FUNCTION: Mitochondrial membrane ATP synthase (F(1)F(o) ATP synthase) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(o) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits (Probable). Subunit alpha does not bear the catalytic high-affinity ATP-binding sites. Contrary to the procyclic, insect form that requires F(1)F(o) ATP synthase for ATP synthesis, the bloodstream form relies on ATP hydrolysis by F(1)F(o) ATP synthase to maintain its mitochondrial membrane potential. KEYWORDS: 3D-structure;ATP synthesis;ATP-binding;CF(1);Direct protein sequencing;Hydrogen ion transport;Ion transport;Membrane;Mitochondrion;Mitochondrion inner membrane;Nucleotide-binding;Transit peptide;Transport SUBCELLULAR LOCATION: Mitochondrion. Mitochondrion inner membrane ; Peripheral membrane protein ; Matrix side " Q9GYI0,"PROTEIN NAMES: Lysine-specific demethylase 7 homolog (ceKDM7A) (JmjC domain-containing protein 1.2) (PHD finger protein 8 homolog) (PHF8 homolog) PROTEIN FAMILY: JHDM1 histone demethylase family, JHDM1D subfamily ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the JHDM1 histone demethylase family. JHDM1D subfamily. FUNCTION: Histone demethylase required for nervous system development. Specifically demethylates dimethylated 'Lys-9', 'Lys-23' and 'Lys-27' (H3K9me2, H3K23me2 and H3K27me2, respectively) of histone H3, thereby playing a central role in histone code. Promotes mitochondrial stress-induced longevity. KEYWORDS: 3D-structure;Alternative splicing;Chromatin regulator;Dioxygenase;Iron;Metal-binding;Nucleus;Oxidoreductase;Reference proteome;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus " Q9GYV5,"PROTEIN NAMES: NF-kappa-B essential modulator (Inhibitor of nuclear factor kappa B kinase subunit gamma) (DmIKKgamma) (NEMO homolog) ORGANISM: Drosophila melanogaster (Fruit fly) FUNCTION: Essential signaling component in transmitting the lipopolysaccharide (LPS) signal leading to cact degradation, which is required for NF-kappa-B (Rel) activation. Required for antibacterial immune response. KEYWORDS: Coiled coil;Cytoplasm;Immunity;Innate immunity;Metal-binding;Nucleus;Reference proteome;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cytoplasm Nucleus " Q9GZM5,"PROTEIN NAMES: Protein YIPF3 (Killer lineage protein 1) (Natural killer cell-specific antigen KLIP1) (YIP1 family member 3) [Cleaved into: Protein YIPF3, 36 kDa form III] PROTEIN FAMILY: YIP1 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the YIP1 family. FUNCTION: Involved in the maintenance of the Golgi structure. May play a role in hematopoiesis. KEYWORDS: Acetylation;Cell membrane;Cytoplasm;Differentiation;Glycoprotein;Golgi apparatus;Membrane;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Cytoplasm. Golgi apparatus, cis-Golgi network membrane ; Multi-pass membrane protein. Note=Localization to the cytoplasm or to the cell membrane is developmentally and ontogenetically regulated. " Q9GZN4,PROTEIN NAMES: Brain-specific serine protease 4 (BSSP-4) (Serine protease 22) (Serine protease 26) (Tryptase epsilon) PROTEIN FAMILY: Peptidase S1 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the peptidase S1 family. FUNCTION: Preferentially cleaves the synthetic substrate H-D-Leu-Thr-Arg-pNA compared to tosyl-Gly-Pro-Arg-pNA. KEYWORDS: Disulfide bond;Glycoprotein;Hydrolase;Protease;Reference proteome;Secreted;Serine protease;Signal SUBCELLULAR LOCATION: Secreted Q9GZQ4,PROTEIN NAMES: Neuromedin-U receptor 2 (NMU-R2) (G-protein coupled receptor FM-4) (G-protein coupled receptor TGR-1) PROTEIN FAMILY: G-protein coupled receptor 1 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the G-protein coupled receptor 1 family. FUNCTION: Receptor for the neuromedin-U and neuromedin-S neuropeptides. KEYWORDS: 3D-structure;Cell membrane;Disulfide bond;G-protein coupled receptor;Glycoprotein;Membrane;Receptor;Reference proteome;Transducer;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Q9GZS9,"PROTEIN NAMES: Carbohydrate sulfotransferase 5 (Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 4-alpha) (GST4-alpha) (Intestinal N-acetylglucosamine-6-O-sulfotransferase) (I-GlcNAc6ST) (Intestinal GlcNAc-6-sulfotransferase) (hIGn6ST) (N-acetylglucosamine 6-O-sulfotransferase 3) (GlcNAc6ST-3) (Gn6st-3) PROTEIN FAMILY: Sulfotransferase 1 family, Gal/GlcNAc/GalNAc subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the sulfotransferase 1 family. Gal/GlcNAc/GalNAc subfamily. FUNCTION: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues and O-linked sugars of mucin-type acceptors. Acts on the non-reducing terminal GlcNAc of short carbohydrate substrates. However, it does not transfer sulfate to longer carbohydrate substrates that have poly-N-acetyllactosamine structures. Has no activity toward keratan. Not involved in generating HEV-expressed ligands for SELL. Its substrate specificity may be influenced by its subcellular location. KEYWORDS: Alternative splicing;Carbohydrate metabolism;Glycoprotein;Golgi apparatus;Membrane;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus membrane ; Single-pass type II membrane protein Note=Golgi membrane, early secretory pathway. " Q9GZU5,"PROTEIN NAMES: Nyctalopin PROTEIN FAMILY: Small leucine-rich proteoglycan (SLRP) family, SLRP class IV subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class IV subfamily. KEYWORDS: Congenital stationary night blindness;Disease variant;Extracellular matrix;Glycoprotein;Leucine-rich repeat;Proteoglycan;Reference proteome;Repeat;Secreted;Sensory transduction;Signal;Vision SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix " Q9GZV1,"PROTEIN NAMES: Ankyrin repeat domain-containing protein 2 (Skeletal muscle ankyrin repeat protein) (hArpp) ORGANISM: Homo sapiens (Human) FUNCTION: Functions as a negative regulator of myocyte differentiation. May interact with both sarcoplasmic structural proteins and nuclear proteins to regulate gene expression during muscle development and in response to muscle stress. KEYWORDS: Alternative splicing;ANK repeat;Cytoplasm;Nucleus;Phosphoprotein;Reference proteome;Repeat SUBCELLULAR LOCATION: Cytoplasm, myofibril, sarcomere, I band Cytoplasm, cytosol. Nucleus. Nucleus, PML body. Note=In the sarcoplasm of differentiated striated muscle cells, where it is cytosolic and enriched in the I band. In nucleus and PML bodies of proliferating and undifferentiated myoblasts. Associates with the euchromatin in the nucleus of myocytes upon muscle stress. " Q9GZV8,"PROTEIN NAMES: PR domain zinc finger protein 14 (PR domain-containing protein 14) PROTEIN FAMILY: Class V-like SAM-binding methyltransferase superfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the class V-like SAM-binding methyltransferase superfamily. FUNCTION: Transcription factor that has both positive and negative roles on transcription. Required for the maintenance of embryonic stem cell identity and the reacquisition of pluripotency in somatic cells. May play an essential role in germ cell development at 2 levels: the reacquisition of potential pluripotency, including SOX2 up-regulation, and successful epigenetic reprogramming, characterized by EHMT1 repression. Its association with CBFA2T2 is required for the functions in pluripotency and germ cell formation (By similarity). Directly up-regulates the expression of pluripotency gene POU5F1 through its proximal enhancer. Binds to the DNA consensus sequence 5'-GGTC[TC]CTAA-3'. KEYWORDS: DNA-binding;Metal-binding;Methyltransferase;Nucleus;Phosphoprotein;Reference proteome;Repeat;S-adenosyl-L-methionine;Transcription;Transcription regulation;Transferase;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus " Q9GZW8,PROTEIN NAMES: Membrane-spanning 4-domains subfamily A member 7 (CD20 antigen-like 4) (CD20/FC-epsilon-RI-beta family member 4) (Four-span transmembrane protein 2) PROTEIN FAMILY: MS4A family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the MS4A family. FUNCTION: May be involved in signal transduction as a component of a multimeric receptor complex. KEYWORDS: Alternative splicing;Membrane;Receptor;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. Q9H079,"PROTEIN NAMES: KATNB1-like protein 1 (Katanin p80 subunit B-like 1) ORGANISM: Homo sapiens (Human) FUNCTION: Regulates microtubule-severing activity of KATNAL1 in a concentration-dependent manner in vitro. KEYWORDS: Cytoplasm;Cytoskeleton;Nucleus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Nucleus Cytoplasm, cytoskeleton, spindle pole Note=Localizes to the spindle poles only during mitosis. Sequestered to the nucleus during interphase. " Q9H0C1,"PROTEIN NAMES: Zinc finger MYND domain-containing protein 12 ORGANISM: Homo sapiens (Human) FUNCTION: Required for sperm flagellum function and male fertility. KEYWORDS: 3D-structure;Cell projection;Cilium;Disease variant;Flagellum;Metal-binding;Reference proteome;Repeat;TPR repeat;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cell projection, cilium, flagellum Note=Found along the full length of the sperm flagellum. " Q9H0P0,PROTEIN NAMES: Cytosolic 5'-nucleotidase 3A (7-methylguanosine phosphate-specific 5'-nucleotidase) (7-methylguanosine nucleotidase) (Cytosolic 5'-nucleotidase 3) (Cytosolic 5'-nucleotidase III) (cN-III) (Pyrimidine 5'-nucleotidase 1) (P5'N-1) (P5N-1) (PN-I) (Uridine 5'-monophosphate hydrolase 1) (p36) PROTEIN FAMILY: Pyrimidine 5'-nucleotidase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the pyrimidine 5'-nucleotidase family. FUNCTION: Nucleotidase which shows specific activity towards cytidine monophosphate (CMP) and 7-methylguanosine monophosphate (m(7)GMP). CMP seems to be the preferred substrate. KEYWORDS: 3D-structure;Alternative splicing;Cytoplasm;Direct protein sequencing;Disease variant;Endoplasmic reticulum;Hydrolase;Magnesium;Metal-binding;Nucleotide metabolism;Nucleotide-binding;Phosphoprotein;Reference proteome;Transferase SUBCELLULAR LOCATION: Cytoplasm.; SUBCELLULAR LOCATION: [Isoform 2]: Endoplasmic reticulum. Q9H116,"PROTEIN NAMES: GDNF-inducible zinc finger protein 1 (Zinc finger and BTB domain-containing protein 23) (Zinc finger protein 336) PROTEIN FAMILY: Krueppel C2H2-type zinc-finger protein family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein family. FUNCTION: Transcriptional repressor that binds the GZF1 responsive element (GRE) (consensus: 5'-TGCGCN[TG][CA]TATA-3'). May be regulating VSX2/HOX10 expression. KEYWORDS: Alternative splicing;Cytoplasm;Disease variant;DNA-binding;Dwarfism;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Repeat;Repressor;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cytoplasm Nucleus, nucleoplasm Nucleus, nucleolus Note=Nuclear localization depends upon NCL. " Q9H161,"PROTEIN NAMES: Homeobox protein aristaless-like 4 PROTEIN FAMILY: Paired homeobox family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the paired homeobox family. FUNCTION: Transcription factor involved in skull and limb development. Plays an essential role in craniofacial development, skin and hair follicle development. KEYWORDS: 3D-structure;Activator;Craniosynostosis;Developmental protein;Disease variant;DNA-binding;Homeobox;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " Q9H1B7,"PROTEIN NAMES: Probable E3 ubiquitin-protein ligase IRF2BPL (Enhanced at puberty protein 1) (Interferon regulatory factor 2-binding protein-like) PROTEIN FAMILY: IRF2BP family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the IRF2BP family. FUNCTION: Probable E3 ubiquitin protein ligase involved in the proteasome-mediated ubiquitin-dependent degradation of target proteins. Through the degradation of CTNNB1, functions downstream of FOXF2 to negatively regulate the Wnt signaling pathway. Probably plays a role in the development of the central nervous system and in neuronal maintenance (Probable). Also acts as a transcriptional regulator of genes controlling female reproductive function. May play a role in gene transcription by transactivating GNRH1 promoter and repressing PENK promoter (By similarity). PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: 3D-structure;Coiled coil;Disease variant;Epilepsy;Intellectual disability;Isopeptide bond;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Transferase;Triplet repeat expansion;Ubl conjugation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus " Q9H1J1,"PROTEIN NAMES: Regulator of nonsense transcripts 3A (Nonsense mRNA reducing factor 3A) (Up-frameshift suppressor 3 homolog A) (hUpf3) PROTEIN FAMILY: RENT3 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the RENT3 family. FUNCTION: Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by associating with the nuclear exon junction complex (EJC) and serving as link between the EJC core and NMD machinery. Recruits UPF2 at the cytoplasmic side of the nuclear envelope and the subsequent formation of an UPF1-UPF2-UPF3 surveillance complex (including UPF1 bound to release factors at the stalled ribosome) is believed to activate NMD. However, UPF3A is shown to be only marginally active in NMD as compared to UPF3B. Binds spliced mRNA upstream of exon-exon junctions. In vitro, weakly stimulates translation. KEYWORDS: 3D-structure;Alternative splicing;Cytoplasm;mRNA transport;Nonsense-mediated mRNA decay;Nucleus;Phosphoprotein;Reference proteome;RNA-binding;Transport SUBCELLULAR LOCATION: Nucleus Cytoplasm Note=Shuttling between the nucleus and the cytoplasm. " Q9H1K0,PROTEIN NAMES: Rabenosyn-5 (110 kDa protein) (FYVE finger-containing Rab5 effector protein rabenosyn-5) (RAB effector RBSN) (Zinc finger FYVE domain-containing protein 20) ORGANISM: Homo sapiens (Human) FUNCTION: Rab4/Rab5 effector protein acting in early endocytic membrane fusion and membrane trafficking of recycling endosomes. Required for endosome fusion either homotypically or with clathrin coated vesicles. Plays a role in the lysosomal trafficking of CTSD/cathepsin D from the Golgi to lysosomes. Also promotes the recycling of transferrin directly from early endosomes to the plasma membrane. Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate (PtdInsP3). Plays a role in the recycling of transferrin receptor to the plasma membrane. KEYWORDS: 3D-structure;Acetylation;Alternative splicing;Cell membrane;Coiled coil;Endosome;Lipoprotein;Membrane;Metal-binding;Phosphoprotein;Protein transport;Reference proteome;Transport;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor; Cytoplasmic side. Early endosome membrane ; Lipid-anchor. Note=Enriched in endosomes that are in close proximity to clathrin-enriched regions at the cell surface. Q9H1Y3,"PROTEIN NAMES: Opsin-3 (Encephalopsin) (Panopsin) PROTEIN FAMILY: G-protein coupled receptor 1 family, Opsin subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the G-protein coupled receptor 1 family. Opsin subfamily. FUNCTION: G-protein coupled receptor which selectively activates G proteins via ultraviolet A (UVA) light-mediated activation in the skin. Binds both 11-cis retinal and all-trans retinal. Regulates melanogenesis in melanocytes via inhibition of alpha-MSH-induced MC1R-mediated cAMP signaling, modulation of calcium flux, regulation of CAMK2 phosphorylation, and subsequently phosphorylation of CREB, p38, ERK and MITF in response to blue light. Plays a role in melanocyte survival through regulation of intracellular calcium levels and subsequent BCL2/RAF1 signaling. Additionally regulates apoptosis via cytochrome c release and subsequent activation of the caspase cascade. Required for TYR and DCT blue light-induced complex formation in melanocytes. Involved in keratinocyte differentiation in response to blue-light. Required for the UVA-mediated induction of calcium and mitogen-activated protein kinase signaling resulting in the expression of MMP1, MMP2, MMP3, MMP9 and TIMP1 in dermal fibroblasts. Plays a role in light-mediated glucose uptake, mitochondrial respiration and fatty acid metabolism in brown adipocyte tissues (By similarity). May be involved in photorelaxation of airway smooth muscle cells, via blue-light dependent GPCR signaling pathways (By similarity). KEYWORDS: Alternative splicing;Cell membrane;Chromophore;Cytoplasm;Disulfide bond;G-protein coupled receptor;Glycoprotein;Lipoprotein;Membrane;Palmitate;Photoreceptor protein;Receptor;Reference proteome;Retinal protein;Sensory transduction;Transducer;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Cytoplasm " Q9H237,"PROTEIN NAMES: Protein-serine O-palmitoleoyltransferase porcupine (Protein MG61) PROTEIN FAMILY: Membrane-bound acyltransferase family, Porcupine subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the membrane-bound acyltransferase family. Porcupine subfamily. FUNCTION: Protein-serine O-palmitoleoyltransferase that acts as a key regulator of the Wnt signaling pathway by mediating the attachment of palmitoleate, a 16-carbon monounsaturated fatty acid (C16:1(9Z)), to Wnt proteins. Serine palmitoleoylation of WNT proteins is required for efficient binding to frizzled receptors. KEYWORDS: 3D-structure;Acyltransferase;Alternative splicing;Disease variant;Endoplasmic reticulum;Lipoprotein;Membrane;Palmitate;Reference proteome;Transferase;Transmembrane;Transmembrane helix;Wnt signaling pathway SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein " Q9H239,"PROTEIN NAMES: Matrix metalloproteinase-28 (MMP-28) (Epilysin) PROTEIN FAMILY: Peptidase M10A family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the peptidase M10A family. FUNCTION: Can degrade casein. Could play a role in tissues homeostasis and repair. KEYWORDS: Alternative splicing;Calcium;Cleavage on pair of basic residues;Disulfide bond;Extracellular matrix;Glycoprotein;Hydrolase;Metal-binding;Metalloprotease;Protease;Reference proteome;Repeat;Secreted;Signal;Zinc;Zymogen SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix. " Q9H2W1,PROTEIN NAMES: Membrane-spanning 4-domains subfamily A member 6A (CD20 antigen-like 3) (Four-span transmembrane protein 3) PROTEIN FAMILY: MS4A family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the MS4A family. FUNCTION: May be involved in signal transduction as a component of a multimeric receptor complex. KEYWORDS: Alternative splicing;Membrane;Receptor;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. Q9H2W2,"PROTEIN NAMES: Homeobox protein MIXL1 (Homeodomain protein MIX) (hMix) (MIX1 homeobox-like protein 1) (Mix.1 homeobox-like protein) PROTEIN FAMILY: Paired homeobox family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the paired homeobox family. FUNCTION: Transcription factor that play a central role in proper axial mesendoderm morphogenesis and endoderm formation. Required for efficient differentiation of cells from the primitive streak stage to blood, by acting early in the recruitment and/or expansion of mesodermal progenitors to the hemangioblastic and hematopoietic lineages. Also involved in the morphogenesis of the heart and the gut during embryogenesis. Acts as a negative regulator of brachyury expression (By similarity). KEYWORDS: Alternative splicing;Developmental protein;Differentiation;DNA-binding;Homeobox;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " Q9H2X0,"PROTEIN NAMES: Chordin PROTEIN FAMILY: Chordin family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the chordin family. FUNCTION: Dorsalizing factor. Key developmental protein that dorsalizes early vertebrate embryonic tissues by binding to ventralizing TGF-beta family bone morphogenetic proteins (BMPs) and sequestering them in latent complexes (By similarity). KEYWORDS: Alternative splicing;Developmental protein;Glycoprotein;Reference proteome;Repeat;Secreted;Signal SUBCELLULAR LOCATION: Secreted MISCELLANEOUS: [Isoform 2]: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.; MISCELLANEOUS: [Isoform 3]: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.; MISCELLANEOUS: [Isoform 4]: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay." Q9H3R2,PROTEIN NAMES: Mucin-13 (MUC-13) (Down-regulated in colon cancer 1) ORGANISM: Homo sapiens (Human) FUNCTION: Epithelial and hemopoietic transmembrane mucin that may play a role in cell signaling. KEYWORDS: Cell membrane;Disulfide bond;EGF-like domain;Glycoprotein;Membrane;Reference proteome;Repeat;Secreted;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein Apical cell membrane Secreted Note=Also exists as a soluble form. Q9H3T3,PROTEIN NAMES: Semaphorin-6B (Semaphorin-Z) (Sema Z) PROTEIN FAMILY: Semaphorin family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the semaphorin family. FUNCTION: Functions as a cell surface repellent for mossy fibers of developing neurons in the hippocampus where it plays a role in axon guidance. May function through the PLXNA4 receptor expressed by mossy cell axons.; FUNCTION: (Microbial infection) Acts as a receptor for P.sordellii toxin TcsL in the in the vascular endothelium. KEYWORDS: Alternative splicing;Cell membrane;Developmental protein;Differentiation;Direct protein sequencing;Disulfide bond;Epilepsy;Glycoprotein;Membrane;Methylation;Neurodegeneration;Neurogenesis;Reference proteome;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein Q9H467,"PROTEIN NAMES: CUE domain-containing protein 2 PROTEIN FAMILY: CUEDC2 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the CUEDC2 family. FUNCTION: Down-regulates ESR1 protein levels through the ubiquitination-proteasome pathway, regardless of the presence of 17 beta-estradiol. Also involved in 17 beta-estradiol-induced ESR1 degradation. Controls PGR protein levels through a similar mechanism. KEYWORDS: Cytoplasm;Nucleus;Phosphoprotein;Reference proteome;Ubl conjugation pathway SUBCELLULAR LOCATION: Cytoplasm Nucleus " Q9H497,"PROTEIN NAMES: Torsin-3A (ATP-dependent interferon-responsive protein) (Torsin family 3 member A) PROTEIN FAMILY: ClpA/ClpB family, Torsin subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the ClpA/ClpB family. Torsin subfamily. KEYWORDS: Alternative splicing;ATP-binding;Cytoplasm;Endoplasmic reticulum;Glycoprotein;Nucleotide-binding;Reference proteome;Signal SUBCELLULAR LOCATION: Cytoplasm. Endoplasmic reticulum lumen. " Q9H4Q4,PROTEIN NAMES: PR domain zinc finger protein 12 (PR domain-containing protein 12) PROTEIN FAMILY: Class V-like SAM-binding methyltransferase superfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the class V-like SAM-binding methyltransferase superfamily. FUNCTION: Involved in the positive regulation of histone H3-K9 dimethylation. KEYWORDS: 3D-structure;Disease variant;DNA-binding;Metal-binding;Methyltransferase;Neurodegeneration;Neuropathy;Nucleus;Reference proteome;Repeat;S-adenosyl-L-methionine;Transcription;Transcription regulation;Transferase;Triplet repeat expansion;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Q9H5Y7,PROTEIN NAMES: SLIT and NTRK-like protein 6 PROTEIN FAMILY: SLITRK family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the SLITRK family. FUNCTION: Regulator of neurite outgrowth required for normal hearing and vision. KEYWORDS: Cell membrane;Deafness;Hearing;Leucine-rich repeat;Membrane;Reference proteome;Repeat;Sensory transduction;Signal;Transmembrane;Transmembrane helix;Vision SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein Q9H6E5,"PROTEIN NAMES: Speckle targeted PIP5K1A-regulated poly(A) polymerase (Star-PAP) (RNA-binding motif protein 21) (RNA-binding protein 21) (U6 snRNA-specific terminal uridylyltransferase 1) (U6-TUTase) PROTEIN FAMILY: DNA polymerase type-B-like family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the DNA polymerase type-B-like family. FUNCTION: Poly(A) polymerase that creates the 3'-poly(A) tail of specific pre-mRNAs. Localizes to nuclear speckles together with PIP5K1A and mediates polyadenylation of a select set of mRNAs, such as HMOX1. In addition to polyadenylation, it is also required for the 3'-end cleavage of pre-mRNAs: binds to the 3'UTR of targeted pre-mRNAs and promotes the recruitment and assembly of the CPSF complex on the 3'UTR of pre-mRNAs. In addition to adenylyltransferase activity, also has uridylyltransferase activity. However, the ATP ratio is higher than UTP in cells, suggesting that it functions primarily as a poly(A) polymerase. Acts as a specific terminal uridylyltransferase for U6 snRNA in vitro: responsible for a controlled elongation reaction that results in the restoration of the four 3'-terminal UMP-residues found in newly transcribed U6 snRNA. Not involved in replication-dependent histone mRNA degradation. KEYWORDS: 3D-structure;ATP-binding;Direct protein sequencing;Magnesium;Manganese;Metal-binding;mRNA processing;Nucleotide-binding;Nucleotidyltransferase;Nucleus;Phosphoprotein;Reference proteome;RNA-binding;Transferase;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus, nucleolus Nucleus speckle " Q9H6K1,"PROTEIN NAMES: Protein ILRUN (Inflammation and lipid regulator with UBA-like and NBR1-like domains protein) ORGANISM: Homo sapiens (Human) FUNCTION: Negative regulator of innate antiviral response. Blocks IRF3-dependent cytokine production such as IFNA, IFNB and TNF. Interacts with IRF3 and inhibits IRF3 recruitment to type I IFN promoter sequences while also reducing nuclear levels of the coactivators EP300 and CREBBP. KEYWORDS: 3D-structure;Alternative splicing;Cytoplasm;Immunity;Innate immunity;Nucleus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Nucleus " Q9H6X2,"PROTEIN NAMES: Anthrax toxin receptor 1 (Tumor endothelial marker 8) PROTEIN FAMILY: ATR family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the ATR family. FUNCTION: Plays a role in cell attachment and migration. Interacts with extracellular matrix proteins and with the actin cytoskeleton. Mediates adhesion of cells to type 1 collagen and gelatin, reorganization of the actin cytoskeleton and promotes cell spreading. Plays a role in the angiogenic response of cultured umbilical vein endothelial cells.; FUNCTION: (Microbial infection) Acts as a receptor for protective antigen (PA) of B.anthracis. KEYWORDS: 3D-structure;Alternative splicing;Cell membrane;Cell projection;Disulfide bond;Glycoprotein;Hypotrichosis;Membrane;Metal-binding;Phosphoprotein;Receptor;Reference proteome;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein Cell projection, lamellipodium membrane ; Single-pass type I membrane protein Cell projection, filopodium membrane ; Single-pass type I membrane protein Note=At the membrane of lamellipodia and at the tip of actin-enriched filopodia. Colocalizes with actin at the base of lamellipodia. MISCELLANEOUS: [Isoform 1]: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.; MISCELLANEOUS: [Isoform 6]: Prostate-specific." Q9H7D7,"PROTEIN NAMES: WD repeat-containing protein 26 (CUL4- and DDB1-associated WDR protein 2) (Myocardial ischemic preconditioning up-regulated protein 2) ORGANISM: Homo sapiens (Human) FUNCTION: G-beta-like protein involved in cell signal transduction. Acts as a negative regulator in MAPK signaling pathway. Functions as a scaffolding protein to promote G beta:gamma-mediated PLCB2 plasma membrane translocation and subsequent activation in leukocytes. Core component of the CTLH E3 ubiquitin-protein ligase complex that selectively accepts ubiquitin from UBE2H and mediates ubiquitination and subsequent proteasomal degradation of the transcription factor HBP1. Acts as a negative regulator of the canonical Wnt signaling pathway through preventing ubiquitination of beta-catenin CTNNB1 by the beta-catenin destruction complex, thus negatively regulating CTNNB1 degradation. Serves as a scaffold to coordinate PI3K/AKT pathway-driven cell growth and migration. Protects cells from oxidative stress-induced apoptosis via the down-regulation of AP-1 transcriptional activity as well as by inhibiting cytochrome c release from mitochondria. Protects also cells by promoting hypoxia-mediated autophagy and mitophagy (By similarity). KEYWORDS: Alternative splicing;Cytoplasm;Disease variant;Intellectual disability;Mitochondrion;Nucleus;Phosphoprotein;Reference proteome;Repeat;WD repeat SUBCELLULAR LOCATION: Cytoplasm Nucleus Mitochondrion " Q9H7M9,"PROTEIN NAMES: V-type immunoglobulin domain-containing suppressor of T-cell activation (Platelet receptor Gi24) (Stress-induced secreted protein-1) (Sisp-1) (V-set domain-containing immunoregulatory receptor) (V-set immunoregulatory receptor) ORGANISM: Homo sapiens (Human) FUNCTION: Immunoregulatory receptor which inhibits the T-cell response. May promote differentiation of embryonic stem cells, by inhibiting BMP4 signaling (By similarity). May stimulate MMP14-mediated MMP2 activation. KEYWORDS: 3D-structure;Cell membrane;Direct protein sequencing;Disulfide bond;Glycoprotein;Immunoglobulin domain;Membrane;Phosphoprotein;Receptor;Reference proteome;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein " Q9H808,"PROTEIN NAMES: Transducin-like enhancer protein 6 PROTEIN FAMILY: WD repeat Groucho/TLE family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the WD repeat Groucho/TLE family. FUNCTION: Regulates spermatogonia proliferation and cell cycle progression, potentially via regulation of cell cycle regulatory genes such as; CEBPB, CEBPA, CSF3, PCNA, and CDK4 (By similarity). Suppresses FOXG1/BF-1-mediated transcriptional repression by inhibiting interaction of the transcriptional corepressor TLE1 with FOXG1 which promotes cortical neuron differentiation (By similarity). Acts as a transcriptional corepressor of NFATC1-mediated gene expression by contributing to PAX6-mediated repression (By similarity).; FUNCTION: [Isoform 1]: As a member of the subcortical maternal complex (SCMC), plays an essential role for zygotes to progress beyond the first embryonic cell divisions via regulation of actin dynamics. Required for the formation of F-actin cytoplasmic lattices in oocytes which in turn are responsible for symmetric division of zygotes via the regulation of mitotic spindle formation and positioning (By similarity). KEYWORDS: Alternative splicing;Cytoplasm;Disease variant;Nucleus;Phosphoprotein;Reference proteome;Repeat;Transcription;Transcription regulation;WD repeat SUBCELLULAR LOCATION: Nucleus.; SUBCELLULAR LOCATION: [Isoform 1]: Cytoplasm.; SUBCELLULAR LOCATION: [Isoform 2]: Cytoplasm " Q9H825,"PROTEIN NAMES: tRNA N(3)-methylcytidine methyltransferase METTL8, mitochondrial (Methyltransferase-like protein 8) (mRNA N(3)-methylcytidine methyltransferase METTL8) PROTEIN FAMILY: Methyltransferase superfamily, METL family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the methyltransferase superfamily. METL family. FUNCTION: Mitochondrial S-adenosyl-L-methionine-dependent methyltransferase that mediates N(3)-methylcytidine modification of residue 32 of the tRNA anticodon loop of mitochondrial tRNA(Ser)(UCN) and tRNA(Thr). N(3)-methylcytidine methylation modification regulates mitochondrial translation efficiency and is required for activity of the respiratory chain. N(3)-methylcytidine methylation of mitochondrial tRNA(Ser)(UCN) requires the formation of N(6)-dimethylallyladenosine(37) (i6A37) by TRIT1 as prerequisite. May also mediate N(3)-methylcytidine modification of mRNAs. The existence of N(3)-methylcytidine modification on mRNAs is however unclear, and additional evidences are required to confirm the role of the N(3)-methylcytidine-specific mRNA methyltransferase activity of METTL8 in vivo. KEYWORDS: Alternative splicing;Isopeptide bond;Methyltransferase;Mitochondrion;Reference proteome;S-adenosyl-L-methionine;Transferase;Transit peptide;tRNA processing;Ubl conjugation SUBCELLULAR LOCATION: Mitochondrion Note=Mitochondrial protein: the cytoplasmic or nuclear localization observed by some groups is either the result of an incorrect localization caused by N-terminal tagging that interferes with mitochondrial targeting, or splice isoforms that lack the N-terminal mitochondrial transit sequence. " Q9H840,"PROTEIN NAMES: Gem-associated protein 7 (Gemin-7) (SIP3) PROTEIN FAMILY: Gemin-7 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the gemin-7 family. FUNCTION: The SMN complex catalyzes the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome, and thereby plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP (Sm core). In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. To assemble core snRNPs, the SMN complex accepts the trapped 5Sm proteins from CLNS1A forming an intermediate. Binding of snRNA inside 5Sm triggers eviction of the SMN complex, thereby allowing binding of SNRPD3 and SNRPB to complete assembly of the core snRNP. KEYWORDS: 3D-structure;Acetylation;Cytoplasm;Direct protein sequencing;mRNA processing;mRNA splicing;Nucleus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Nucleus, nucleoplasm Nucleus, gem Cytoplasm Note=Found both in the nucleoplasm and in nuclear bodies called gems (Gemini of Cajal bodies) that are often in proximity to Cajal (coiled) bodies. Also found in the cytoplasm. " Q9H892,"PROTEIN NAMES: Tetratricopeptide repeat protein 12 (TPR repeat protein 12) ORGANISM: Homo sapiens (Human) FUNCTION: Cytoplasmic protein that plays a role in the proper assembly of dynein arm complexes in motile cilia in both respiratory cells and sperm flagella. KEYWORDS: Alternative splicing;Ciliopathy;Cilium biogenesis/degradation;Cytoplasm;Disease variant;Phosphoprotein;Primary ciliary dyskinesia;Reference proteome;Repeat;TPR repeat SUBCELLULAR LOCATION: Cytoplasm MISCELLANEOUS: [Isoform 2]: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay." Q9H8H2,"PROTEIN NAMES: ATP-dependent DNA helicase DDX31 (DEAD box protein 31) (Helicain) (Probable ATP-dependent RNA helicase DDX31) PROTEIN FAMILY: DEAD box helicase family, DDX31/DBP7 subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the DEAD box helicase family. DDX31/DBP7 subfamily. FUNCTION: May have DNA helicase activity and RNA helicase activity. Probably have ssDNA and RNA dependent ATPase activity (By similarity). Plays a role in ribosome biogenesis and TP53/p53 regulation through its interaction with NPM1. KEYWORDS: Alternative initiation;Alternative splicing;ATP-binding;Helicase;Hydrolase;Methylation;Nucleotide-binding;Nucleus;Reference proteome;RNA-binding SUBCELLULAR LOCATION: Nucleus, nucleolus Note=Colocalized with NPM1 in the nucleoli. " Q9H8K7,"PROTEIN NAMES: ATPase PAAT (Protein associated with ABC transporters) (PAAT) ORGANISM: Homo sapiens (Human) FUNCTION: ATPase that regulates mitochondrial ABC transporters ABCB7, ABCB8/MITOSUR and ABCB10. Regulates mitochondrial ferric concentration and heme biosynthesis and plays a role in the maintenance of mitochondrial homeostasis and cell survival. KEYWORDS: Cytoplasm;Hydrolase;Mitochondrion;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Mitochondrion " Q9H8X2,"PROTEIN NAMES: Inositol-pentakisphosphate 2-kinase (IPK1 homolog) (Inositol-1,3,4,5,6-pentakisphosphate 2-kinase) (Ins(1,3,4,5,6)P5 2-kinase) (InsP5 2-kinase) PROTEIN FAMILY: IPK1 type 2 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the IPK1 type 2 family. FUNCTION: Phosphorylates Ins(1,3,4,5,6)P5 at position 2 to form Ins(1,2,3,4,5,6)P6 (InsP6 or phytate). InsP6 is involved in many processes such as mRNA export, non-homologous end-joining, endocytosis, ion channel regulation. It also protects cells from TNF-alpha-induced apoptosis. KEYWORDS: ATP-binding;Cytoplasm;Kinase;Nucleotide-binding;Nucleus;Phosphoprotein;Reference proteome;Transferase SUBCELLULAR LOCATION: Cytoplasm Nucleus " Q9H987,"PROTEIN NAMES: Synaptopodin 2-like protein PROTEIN FAMILY: Synaptopodin family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the synaptopodin family. FUNCTION: Actin-associated protein that may play a role in modulating actin-based shape. KEYWORDS: Actin-binding;Alternative splicing;Cytoplasm;Cytoskeleton;Methylation;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton " Q9H9C1,"PROTEIN NAMES: Spermatogenesis-defective protein 39 homolog (hSPE-39) (VPS33B-interacting protein in apical-basolateral polarity regulator) (VPS33B-interacting protein in polarity and apical restriction) PROTEIN FAMILY: SPE39 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the SPE39 family. FUNCTION: Proposed to be involved in endosomal maturation implicating in part VPS33B. In epithelial cells, the VPS33B:VIPAS39 complex may play a role in the apical RAB11A-dependent recycling pathway and in the maintenance of the apical-basolateral polarity. May play a role in lysosomal trafficking, probably via association with the core HOPS complex in a discrete population of endosomes; the functions seems to be independent of VPS33B. May play a role in vesicular trafficking during spermatogenesis (By similarity). May be involved in direct or indirect transcriptional regulation of E-cadherin (By similarity). KEYWORDS: Alternative splicing;Cytoplasm;Cytoplasmic vesicle;Differentiation;Disease variant;Endosome;Phosphoprotein;Protein transport;Reference proteome;Spermatogenesis;Transcription;Transcription regulation;Transport SUBCELLULAR LOCATION: Cytoplasm Cytoplasmic vesicle Early endosome Recycling endosome Late endosome Note=Colocalizes in clusters with VPS33B at cytoplasmic organelles. " Q9H9D4,PROTEIN NAMES: Zinc finger protein 408 (PR domain zinc finger protein 17) ORGANISM: Homo sapiens (Human) FUNCTION: May be involved in transcriptional regulation. KEYWORDS: Disease variant;DNA-binding;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Repeat;Retinitis pigmentosa;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Q9H9S5,"PROTEIN NAMES: Ribitol 5-phosphate transferase FKRP (Fukutin-related protein) (Ribitol-5-phosphate transferase) PROTEIN FAMILY: LicD transferase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the LicD transferase family. FUNCTION: Catalyzes the transfer of a ribitol 5-phosphate from CDP-L-ribitol to the ribitol 5-phosphate previously attached by FKTN/fukutin to the phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-3-N-acetylglucosamine-beta-4-(phosphate-6-)mannose), a carbohydrate structure present in alpha-dystroglycan (DAG1). This constitutes the second step in the formation of the ribose 5-phosphate tandem repeat which links the phosphorylated O-mannosyl trisaccharide to the ligand binding moiety composed of repeats of 3-xylosyl-alpha-1,3-glucuronic acid-beta-1. PATHWAY: Protein modification; protein glycosylation. KEYWORDS: 3D-structure;Cardiomyopathy;Cell membrane;Congenital muscular dystrophy;Cytoplasm;Disease variant;Disulfide bond;Dystroglycanopathy;Endoplasmic reticulum;Glycoprotein;Golgi apparatus;Limb-girdle muscular dystrophy;Lissencephaly;Magnesium;Membrane;Metal-binding;Reference proteome;Secreted;Signal-anchor;Transferase;Transmembrane;Transmembrane helix;Zinc SUBCELLULAR LOCATION: Golgi apparatus membrane ; Single-pass type II membrane protein Secreted Cell membrane, sarcolemma Rough endoplasmic reticulum Cytoplasm Note=According to some studies the N-terminal hydrophobic domain is cleaved after translocation to the Golgi apparatus and the protein is secreted. Localization at the cell membrane may require the presence of dystroglycan (By similarity). At the Golgi apparatus localizes to the middle-to-trans-cisternae, as assessed by MG160 colocalization. Detected in rough endoplasmic reticulum in myocytes. In general, mutants associated with severe clinical phenotypes are retained within the endoplasmic reticulum. " Q9H9V9,PROTEIN NAMES: 2-oxoglutarate and iron-dependent oxygenase JMJD4 (JmjC domain-containing protein 4) (Jumonji domain-containing protein 4) (Lysyl-hydroxylase JMJD4) ORGANISM: Homo sapiens (Human) FUNCTION: Catalyzes the 2-oxoglutarate and iron-dependent C4-lysyl hydroxylation of ETF1 at 'Lys-63' thereby promoting the translational termination efficiency of ETF1. KEYWORDS: Alternative initiation;Alternative splicing;Cytoplasm;Dioxygenase;Iron;Metal-binding;Oxidoreductase;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Q9HA65,"PROTEIN NAMES: TBC1 domain family member 17 ORGANISM: Homo sapiens (Human) FUNCTION: Probable RAB GTPase-activating protein that inhibits RAB8A/B function. Reduces Rab8 recruitment to tubules emanating from the endocytic recycling compartment (ERC) and inhibits Rab8-mediated endocytic trafficking, such as that of transferrin receptor (TfR). Involved in regulation of autophagy. KEYWORDS: Alternative splicing;Autophagy;Cytoplasm;Cytoplasmic vesicle;Endosome;GTPase activation;Phosphoprotein;Protein transport;Reference proteome;Transport SUBCELLULAR LOCATION: Cytoplasmic vesicle, autophagosome Cytoplasm Recycling endosome Note=In the presence of optineurin/OPTN, may be recruited to recycling endosomes. " Q9HAS0,"PROTEIN NAMES: Protein Njmu-R1 ORGANISM: Homo sapiens (Human) FUNCTION: As component of the WDR11 complex acts together with TBC1D23 to facilitate the golgin-mediated capture of vesicles generated using AP-1. May have a role in spermatogenesis. KEYWORDS: Cytoplasmic vesicle;Golgi apparatus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Golgi apparatus, trans-Golgi network Cytoplasmic vesicle " Q9HB29,"PROTEIN NAMES: Interleukin-1 receptor-like 2 (IL-36 receptor) (IL-36R) (Interleukin-1 receptor-related protein 2) (IL-1Rrp2) (IL1R-rp2) PROTEIN FAMILY: Interleukin-1 receptor family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the interleukin-1 receptor family. FUNCTION: Receptor for interleukin-36 (IL36A, IL36B and IL36G). After binding to interleukin-36 associates with the coreceptor IL1RAP to form the interleukin-36 receptor complex which mediates interleukin-36-dependent activation of NF-kappa-B, MAPK and other pathways (By similarity). The IL-36 signaling system is thought to be present in epithelial barriers and to take part in local inflammatory response; it is similar to the IL-1 system. Seems to be involved in skin inflammatory response by induction of the IL-23/IL-17/IL-22 pathway. KEYWORDS: 3D-structure;Alternative splicing;Disulfide bond;Glycoprotein;Hydrolase;Immunity;Immunoglobulin domain;Inflammatory response;Innate immunity;Membrane;NAD;Receptor;Reference proteome;Repeat;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein " Q9HB65,"PROTEIN NAMES: RNA polymerase II elongation factor ELL3 PROTEIN FAMILY: ELL/occludin family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the ELL/occludin family. FUNCTION: Enhancer-binding elongation factor that specifically binds enhancers in embryonic stem cells (ES cells), marks them, and is required for their future activation during stem cell specification. Does not only bind to enhancer regions of active genes, but also marks the enhancers that are in a poised or inactive state in ES cells and is required for establishing proper RNA polymerase II occupancy at developmentally regulated genes in a cohesin-dependent manner. Probably required for priming developmentally regulated genes for later recruitment of the super elongation complex (SEC), for transcriptional activation during differentiation. Required for recruitment of P-TEFb within SEC during differentiation. Probably preloaded on germ cell chromatin, suggesting that it may prime gene activation by marking enhancers as early as in the germ cells. Promoting epithelial-mesenchymal transition (EMT) (By similarity). Elongation factor component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. Component of the little elongation complex (LEC), a complex required to regulate small nuclear RNA (snRNA) gene transcription by RNA polymerase II and III. KEYWORDS: Alternative splicing;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " Q9HBE4,"PROTEIN NAMES: Interleukin-21 (IL-21) (Za11) PROTEIN FAMILY: IL-15/IL-21 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the IL-15/IL-21 family. FUNCTION: Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG(1) and IgG(3) in B-cells (By similarity). Implicated in the generation and maintenance of T follicular helper (Tfh) cells and the formation of germinal-centers. Together with IL6, control the early generation of Tfh cells and are critical for an effective antibody response to acute viral infection (By similarity). May play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15. May regulate proliferation of mature B- and T-cells in response to activating stimuli. In synergy with IL15 and IL18 stimulates interferon gamma production in T-cells and NK cells. During T-cell mediated immune response may inhibit dendritic cells (DC) activation and maturation (By similarity). KEYWORDS: 3D-structure;Alternative splicing;Cytokine;Disease variant;Disulfide bond;Glycoprotein;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted " Q9HBE5,"PROTEIN NAMES: Interleukin-21 receptor (IL-21 receptor) (IL-21R) (Novel interleukin receptor) (CD antigen CD360) PROTEIN FAMILY: Type I cytokine receptor family, Type 4 subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the type I cytokine receptor family. Type 4 subfamily. FUNCTION: This is a receptor for interleukin-21. KEYWORDS: 3D-structure;Chromosomal rearrangement;Direct protein sequencing;Disease variant;Disulfide bond;Glycoprotein;Membrane;Receptor;Reference proteome;Repeat;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. " Q9HBU6,PROTEIN NAMES: Ethanolamine kinase 1 (EKI 1) PROTEIN FAMILY: Choline/ethanolamine kinase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the choline/ethanolamine kinase family. FUNCTION: Highly specific for ethanolamine phosphorylation. May be a rate-controlling step in phosphatidylethanolamine biosynthesis. PATHWAY: Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from ethanolamine: step 1/3. KEYWORDS: Alternative splicing;ATP-binding;Cytoplasm;Kinase;Lipid biosynthesis;Lipid metabolism;Nucleotide-binding;Phospholipid biosynthesis;Phospholipid metabolism;Reference proteome;Transferase SUBCELLULAR LOCATION: Cytoplasm Q9HC36,"PROTEIN NAMES: rRNA methyltransferase 3, mitochondrial (16S rRNA (guanosine(1370)-2'-O)-methyltransferase) (16S rRNA [Gm1370] 2'-O-methyltransferase) (RNA methyltransferase-like protein 1) PROTEIN FAMILY: Class IV-like SAM-binding methyltransferase superfamily, RNA methyltransferase TrmH family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family. FUNCTION: S-adenosyl-L-methionine-dependent 2'-O-ribose methyltransferase that catalyzes the formation of 2'-O-methylguanosine at position 1370 (Gm1370) in the 16S mitochondrial large subunit ribosomal RNA (mtLSU rRNA), a conserved modification in the peptidyl transferase domain of the mtLSU rRNA. KEYWORDS: 3D-structure;Methyltransferase;Mitochondrion;Reference proteome;rRNA processing;S-adenosyl-L-methionine;Transferase;Transit peptide SUBCELLULAR LOCATION: Mitochondrion " Q9HC52,"PROTEIN NAMES: Chromobox protein homolog 8 (Polycomb 3 homolog) (Pc3) (hPc3) (Rectachrome 1) ORGANISM: Homo sapiens (Human) FUNCTION: Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. KEYWORDS: 3D-structure;Chromatin regulator;Nucleus;Phosphoprotein;Reference proteome;Repressor;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: The human orthologuous proteins of Drosophila Polycomb group protein Pc, CBX2, CBX4, CBX6, CBX7 and CBX8, show distinct nuclear localizations, contribute differently to transcriptional repression, and appear to be part of distinct PRC1-like protein complexes. The hPRC-H complex purification reported by previous research probably presents a mixture of different complexes." Q9HC73,"PROTEIN NAMES: Cytokine receptor-like factor 2 (Cytokine receptor-like 2) (IL-XR) (Thymic stromal lymphopoietin protein receptor) (TSLP receptor) PROTEIN FAMILY: Type I cytokine receptor family, Type 5 subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the type I cytokine receptor family. Type 5 subfamily. FUNCTION: Receptor for thymic stromal lymphopoietin (TSLP). Forms a functional complex with TSLP and IL7R which is capable of stimulating cell proliferation through activation of STAT3 and STAT5. Also activates JAK2 (By similarity). Implicated in the development of the hematopoietic system. KEYWORDS: 3D-structure;Alternative splicing;Cell membrane;Disulfide bond;Glycoprotein;Membrane;Receptor;Reference proteome;Secreted;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: [Isoform 1]: Cell membrane ; Single-pass type I membrane protein.; SUBCELLULAR LOCATION: [Isoform 2]: Secreted MISCELLANEOUS: The gene coding for this protein is located in the pseudoautosomal region 1 (PAR1) of X and Y chromosomes." Q9HCG7,"PROTEIN NAMES: Non-lysosomal glucosylceramidase (NLGase) (Beta-glucocerebrosidase 2) (Beta-glucosidase 2) (Bile acid beta-glucosidase GBA2) (Bile acid glucosyl transferase GBA2) (Cholesterol glucosyltransferase GBA2) (Cholesteryl-beta-glucosidase GBA2) (Glucosylceramidase 2) (Non-lysosomal cholesterol glycosyltransferase) (Non-lysosomal galactosylceramidase) (Non-lysosomal glycosylceramidase) PROTEIN FAMILY: Non-lysosomal glucosylceramidase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the non-lysosomal glucosylceramidase family. FUNCTION: Non-lysosomal glucosylceramidase that catalyzes the hydrolysis of glucosylceramides/GlcCers (such as beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine) to free glucose and ceramides (such as N-acylsphing-4-enine). GlcCers are membrane glycosphingolipids that have a wide intracellular distribution (By similarity). They are the main precursors of more complex glycosphingolipids that play a role in cellular growth, differentiation, adhesion, signaling, cytoskeletal dynamics and membrane properties (By similarity). Involved in the transglucosylation of cholesterol, transfers glucose from GlcCer to cholesterol, thereby modifying its water solubility and biological properties. Under specific conditions, may catalyze the reverse reaction, transferring glucose from cholesteryl-3-beta-D-glucoside to ceramide (such as N-acylsphing-4-enine) (Probable). May play a role in the metabolism of bile acids. Able to hydrolyze bile acid 3-O-glucosides as well as to produce bile acid-glucose conjugates thanks to a bile acid glucosyl transferase activity. Catalyzes the hydrolysis of galactosylceramides/GalCers (such as beta-D-galactosyl-(1<->1')-N-acylsphing-4-enine), as well as the galactosyl transfer between GalCers and cholesterol in vitro with lower activity compared with their activity against GlcCers. PATHWAY: Lipid metabolism; sphingolipid metabolism.; PATHWAY: Steroid metabolism; cholesterol metabolism. KEYWORDS: Alternative splicing;Cholesterol metabolism;Direct protein sequencing;Disease variant;Endoplasmic reticulum;Glycosidase;Glycosyltransferase;Golgi apparatus;Hereditary spastic paraplegia;Hydrolase;Lipid metabolism;Membrane;Neurodegeneration;Reference proteome;Sphingolipid metabolism;Steroid metabolism;Sterol metabolism;Transferase SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Peripheral membrane protein ; Cytoplasmic side Golgi apparatus membrane ; Peripheral membrane protein ; Cytoplasmic side Note=Localization to the plasma membrane and alternative topologies have also been reported. " Q9HCK0,PROTEIN NAMES: Zinc finger and BTB domain-containing protein 26 (Zinc finger protein 481) (Zinc finger protein Bioref) ORGANISM: Homo sapiens (Human) FUNCTION: May be involved in transcriptional regulation. KEYWORDS: DNA-binding;Isopeptide bond;Metal-binding;Nucleus;Reference proteome;Repeat;Transcription;Transcription regulation;Ubl conjugation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Q9HCM4,"PROTEIN NAMES: Band 4.1-like protein 5 (Erythrocyte membrane protein band 4.1-like 5) ORGANISM: Homo sapiens (Human) FUNCTION: Plays a role in the formation and organization of tight junctions during the establishment of polarity in epithelial cells. KEYWORDS: Alternative splicing;Cell junction;Cell membrane;Cytoplasm;Membrane;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Cell junction, adherens junction Cell membrane ; Peripheral membrane protein Photoreceptor inner segment " Q9HCU5,"PROTEIN NAMES: Guanine nucleotide-exchange factor SEC12 (Prolactin regulatory element-binding protein) ORGANISM: Homo sapiens (Human) FUNCTION: Guanine nucleotide exchange factor (GEF) that regulates the assembly of the coat protein complex II/COPII in endoplasmic reticulum (ER) to Golgi vesicle-mediated transport. Selectively activates SAR1A and SAR1B by promoting the exchange of guanosine diphosphate (GDP) for guanosine triphosphate (GTP) in these small GTPases. In their activated GTP-bound state, SAR1A and SAR1B insert into the membrane of the endoplasmic reticulum where they recruit the remainder of the coat protein complex II/COPII which is responsible for both the sorting of proteins and the deformation and budding of membranes into vesicles destined to the Golgi.; FUNCTION: Was first identified based on its probable role in the regulation of pituitary gene transcription. Binds to the prolactin gene (PRL) promoter and seems to activate transcription. KEYWORDS: 3D-structure;Activator;DNA-binding;Endoplasmic reticulum;ER-Golgi transport;Membrane;Nitration;Nucleus;Protein transport;Reference proteome;Repeat;Transcription;Transcription regulation;Transmembrane;Transmembrane helix;Transport;WD repeat SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Single-pass membrane protein Nucleus Note=Concentrates at endoplasmic reticulum exit sites (ERES), also known as transitional endoplasmic reticulum (tER). " Q9HGK9,"PROTEIN NAMES: Inner kinetochore subunit cnp20 (CENP-T homolog) (Centromere protein 20) (Constitutive centromere-associated network protein cnp20) PROTEIN FAMILY: CENP-T/CNN1 family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the CENP-T/CNN1 family. FUNCTION: Component of the kinetochore, a multiprotein complex that assembles on centromeric DNA and attaches chromosomes to spindle microtubules, mediating chromosome segregation and sister chromatid segregation during meiosis and mitosis. Component of the inner kinetochore constitutive centromere-associated network (CCAN), which serves as a structural platform for outer kinetochore assembly. KEYWORDS: Chromosome;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus. Chromosome. " Q9HGQ3,"PROTEIN NAMES: Probable zinc transporter cis4 PROTEIN FAMILY: Cation diffusion facilitator (CDF) transporter family, SLC30A subfamily ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. SLC30A subfamily. FUNCTION: Probable zinc transporter involved in Golgi membrane trafficking through the regulation of zinc homeostasis. KEYWORDS: Endoplasmic reticulum;Golgi apparatus;Ion transport;Membrane;Reference proteome;Transmembrane;Transmembrane helix;Transport;Zinc;Zinc transport SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus, cis-Golgi network membrane; Multi-pass membrane protein. " Q9HHB6,"PROTEIN NAMES: Galactokinase (Galactose kinase) PROTEIN FAMILY: GHMP kinase family, GalK subfamily ORGANISM: Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) SIMILARITY: Belongs to the GHMP kinase family. GalK subfamily. FUNCTION: Catalyzes the transfer of the gamma-phosphate of ATP to D-galactose to form alpha-D-galactose-1-phosphate (Gal-1-P). Is very specific for its substrate, since it is not able to use D-glucose, D-fructose, D-mannose, 2-deoxy-D-glucose, and D-glucosamine as substrates. PATHWAY: Carbohydrate metabolism; galactose metabolism. KEYWORDS: 3D-structure;ATP-binding;Carbohydrate metabolism;Cytoplasm;Galactose metabolism;Kinase;Magnesium;Metal-binding;Nucleotide-binding;Reference proteome;Transferase SUBCELLULAR LOCATION: Cytoplasm " Q9HIC2,PROTEIN NAMES: Bifunctional phosphoglucose/phosphomannose isomerase (Glucose-6-phosphate isomerase) (GPI) (Mannose-6-phosphate isomerase) (Phosphoglucose isomerase) (PGI) (Phosphomannose isomerase) (PMI) PROTEIN FAMILY: PGI/PMI family ORGANISM: Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165) SIMILARITY: Belongs to the PGI/PMI family. FUNCTION: Catalyzes the isomerization of both glucose 6-phosphate and epimeric mannose 6-phosphate at a similar catalytic efficiency. KEYWORDS: Isomerase;Multifunctional enzyme;Reference proteome Q9HIN1,"PROTEIN NAMES: Mevalonate 3-kinase (M3K) (ATP:(R)-mevalonate 3-phosphotransferase) (ATP:(R)-MVA 3 phosphotransferase) PROTEIN FAMILY: GHMP kinase family ORGANISM: Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165) SIMILARITY: Belongs to the GHMP kinase family. FUNCTION: Catalyzes the phosphorylation of mevalonate (MVA) to yield mevalonate-3-phosphate. Functions in an alternative mevalonate pathway, only present in extreme acidophiles of the Thermoplasmatales order, which passes through mevalonate 3-phosphate rather than mevalonate 5-phosphate. PATHWAY: Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via mevalonate pathway. KEYWORDS: 3D-structure;ATP-binding;Isoprene biosynthesis;Kinase;Lipid biosynthesis;Lipid metabolism;Nucleotide-binding;Reference proteome;Transferase " Q9HJT3,"PROTEIN NAMES: Nucleoside kinase (NK) (Adenosine kinase) (Broad specificity nucleoside kinase) (Cytidine kinase) (Guanosine-inosine kinase) PROTEIN FAMILY: Carbohydrate kinase PfkB family ORGANISM: Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165) SIMILARITY: Belongs to the carbohydrate kinase PfkB family. FUNCTION: Nucleoside kinase with broad substrate specificity. Catalyzes the phosphorylation of a variety of nucleosides to the corresponding nucleoside 5'-mono-phosphate in the presence of phosphate donors and divalent cations. Displays the most efficient activity with guanosine, followed by inosine, cytidine, and adenosine. Negligible enzymatic activity is detected with thymidine, uridine, and 2-deoxyadenosine. ATP is the most efficient phosphate donor, but can also use GTP and ITP. Shows no sugar kinase activity, since it is unable to phosphorylate ribose, fructose-1-phosphate, or fructose-6-phosphate. KEYWORDS: 3D-structure;ATP-binding;Cobalt;GTP-binding;Kinase;Magnesium;Nucleotide-binding;Reference proteome;Transferase " Q9HKT1,"PROTEIN NAMES: Lipoate-protein ligase A subunit 1 (Lipoate--protein ligase subunit 1) PROTEIN FAMILY: LplA family ORGANISM: Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165) SIMILARITY: Belongs to the LplA family. FUNCTION: Part of a lipoate-protein ligase complex that catalyzes both the ATP-dependent activation of exogenously supplied lipoate to lipoyl-AMP and the transfer of the activated lipoyl onto the lipoyl domains of lipoate-dependent enzymes. Can also use octanoate as substrate. PATHWAY: Protein modification; protein lipoylation via exogenous pathway; protein N(6)-(lipoyl)lysine from lipoate: step 1/2.; PATHWAY: Protein modification; protein lipoylation via exogenous pathway; protein N(6)-(lipoyl)lysine from lipoate: step 2/2. KEYWORDS: 3D-structure;ATP-binding;Cytoplasm;Direct protein sequencing;Ligase;Magnesium;Metal-binding;Nucleotide-binding;Reference proteome SUBCELLULAR LOCATION: Cytoplasm MISCELLANEOUS: In contrast to E.coli, where the lipoate-protein ligase is encoded by a single gene product (LplA) with a large N-terminal domain and a small C-terminal domain, the same activity in T.acidophilum is dependent on two separate proteins, corresponding to the two domains of E.coli LplA, respectively." Q9HTC0,"PROTEIN NAMES: D-rhamnosyltransferase WbpZ (GDP-D-Man:GlcNAc-diphosphate-lipid alpha-1,3-D-mannosyltransferase) (GDP-D-Man:GlcNAc/GalNAc-diphosphate-lipid alpha-1,3-D-mannosyltransferase) (GDP-D-rhamnose:GlcNAc/GalNAc-diphosphate-lipid alpha-1,3-D-rhamnosyltransferase) PROTEIN FAMILY: Glycosyltransferase group 1 family, Glycosyltransferase 4 subfamily ORGANISM: Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) SIMILARITY: Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 4 subfamily. FUNCTION: Non-processive alpha-1,3-D-rhamnosyltransferase. Catalyzes the transfer of one D-rhamnose (D-Rha) residue from donor substrate GDP-D-Rha in alpha-1-3 linkage to both GlcNAc- and GalNAc-diphosphate-lipid acceptor substrates. Is also able to transfer D-mannose (D-Man) to these acceptors at a lower level. Nucleotide sugars GDP-D-Rha, GDP-Fuc, UDP-Gal, UDP-GalNAc, UDP-GlcNAc and CMP-sialic acid cannot act as donor substrates. Only compounds with a diphosphate as the aglycone group can act as acceptor substrates. No activity is detected with compounds containing a diphosphate mimic. Fluorescent undecyl-anthracenyl group-containing compounds, such as GlcNAc-PO(3)-PO(3)-AnthrU and GalNAc-PO(3)-PO(3)-AnthrU, are also good acceptor substrates. Involved in the biosynthesis of the common polysaccharide antigen (CPA), also called A band, which is one of the two major cell surface O-antigens of the P.aeruginosa lipopolysaccharide. Involved in susceptibility to antibiotic colistin. PATHWAY: Lipopolysaccharide biosynthesis; LPS oligosaccharide biosynthesis. KEYWORDS: Antibiotic resistance;Cytoplasm;Glycosyltransferase;Lipopolysaccharide biosynthesis;Reference proteome;Transferase SUBCELLULAR LOCATION: Cytoplasm " Q9HV27,"PROTEIN NAMES: Cyclic di-GMP phosphodiesterase PA4781 ORGANISM: Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) FUNCTION: Phosphodiesterase (PDE) that catalyzes the hydrolysis of cyclic diguanylate (c-di-GMP) to GMP. Hydrolyzes c-di-GMP to GMP in a two-step reaction, via the linear intermediate 5'-phosphoguanylyl(3'->5')guanosine (pGpG). In vitro, can use pGpG as an alternative substrate and hydrolyze it into GMP. Acts in regulation of motility, synthesis of virulence determinants and biofilm architecture. May act preferentially as a pGpG binding protein. KEYWORDS: 3D-structure;c-di-GMP;Hydrolase;Metal-binding;Phosphoprotein;Reference proteome " Q9HXE3,"PROTEIN NAMES: FAD-dependent catabolic D-arginine dehydrogenase DauA (D-arginine dehydrogenase) (DADH) (D-arginine utilization protein A) (Dau) PROTEIN FAMILY: FAD-dependent glycerol-3-phosphate dehydrogenase family ORGANISM: Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) SIMILARITY: Belongs to the FAD-dependent glycerol-3-phosphate dehydrogenase family. FUNCTION: DauA is highly expressed within the cystic fibrosis (CF) lung, and it is required for virulence via the optimal production of hydrogen cyanide, pyocyanine, pyoverdine, rhamnolipid and alginate during biofilm formation. Involved in the catabolism of D-lysine and D-arginine. Under aerobic conditions, the arginine succinyltransferase (AST) and arginine transaminase (ATA) pathways are 2 major routes for L-arginine utilization as the sole source of carbon and nitrogen. The D-to-L racemization of arginine by DauA and DauB is necessary, before to be channeled into the AST and/or ATA pathways. DauA catalyzes the flavin-dependent oxidative deamination of D-arginine into 2-ketoarginine (2-KA) and ammonia. It has also dehydrogenase activity towards D-lysine, D-tyrosine, D-methionine, D-phenylalanine, D-ornithine, D-histidine and D-leucine as substrates. KEYWORDS: 3D-structure;FAD;Flavoprotein;Nucleotide-binding;Oxidoreductase;Reference proteome;Virulence MISCELLANEOUS: In vitro, it is essential to include phenazine methosulfate (PMS) or iodonitrotetrazolium chloride (INT) as the artificial electron acceptor in the reaction to ensure DauA remains active with FAD." Q9HXM1,"PROTEIN NAMES: Histone deacetylase-like amidohydrolase (HDAH) (Acetylated lysine deacetylase) (Histone deacetylase homolog PA3774) PROTEIN FAMILY: Histone deacetylase family ORGANISM: Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) SIMILARITY: Belongs to the histone deacetylase family. FUNCTION: Probable protein deacetylase that catalyzes deacetylation of acetylated lysine residues. In vitro, exhibits high activity against artificial HDAC (histone deacetylase) substrates containing acetylated and trifluoroacetylated lysine residues. Is not able to deacetylate acetylated polyamines. KEYWORDS: 3D-structure;Hydrolase;Metal-binding;Reference proteome;Zinc " Q9I1K1,"PROTEIN NAMES: Probable peptidoglycan D,D-transpeptidase PbpC (PBP3x) (Penicillin-binding protein 3 homolog) (PBP3 homolog) PROTEIN FAMILY: Transpeptidase family, FtsI subfamily ORGANISM: Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) SIMILARITY: Belongs to the transpeptidase family. FtsI subfamily. FUNCTION: Catalyzes cross-linking of the peptidoglycan cell wall at the division septum (By similarity). Binds penicillin. PATHWAY: Cell wall biogenesis; peptidoglycan biosynthesis. KEYWORDS: Carboxypeptidase;Cell cycle;Cell division;Cell inner membrane;Cell membrane;Cell shape;Cell wall biogenesis/degradation;Direct protein sequencing;Hydrolase;Membrane;Peptidoglycan synthesis;Protease;Reference proteome;Septation;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell inner membrane ; Single-pass membrane protein " Q9I3T5,"PROTEIN NAMES: Acetylpolyamine amidohydrolase 1 (APAH 1) (Acetylcadaverine deacetylase) (Acetylpolyamine deacetylase) (Acetylputrescine deacetylase) PROTEIN FAMILY: Histone deacetylase family ORGANISM: Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) SIMILARITY: Belongs to the histone deacetylase family. FUNCTION: Catalyzes the deacetylation of acetylated polyamines such as N-acetylputrescine, N-acetylcadaverine, N(1)-acetylspermine and N(1)-acetylspermidine. Plays an important role in the metabolism of acetylated polyamines in P.aeruginosa. Is involved in the degradation pathways of N-acetylputrescine and N-acetylcadaverine, that allow P.aeruginosa to utilize these acetylpolyamines as a carbon source under glucose starvation. In vitro, can also hydrolyze artificial trifluoroacetylated and acetylated lysine-derivatives. PATHWAY: Amine and polyamine metabolism. KEYWORDS: Hydrolase;Metal-binding;Reference proteome;Zinc " Q9I5W4,"PROTEIN NAMES: Immunomodulating metalloprotease (IMPa) PROTEIN FAMILY: Peptidase M88 family ORGANISM: Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) SIMILARITY: Belongs to the peptidase M88 family. FUNCTION: Protease that degrades several proteins of the host immune system. Cleaves P-selectin glycoprotein ligand-1 (PSGL-1), leading to its functional inhibition; PSGL-1 is a leukocyte cell-surface receptor essential for leukocyte recruitment to the site of infection. Next to PSGL-1, targets host CD43 and CD44 that are also involved in leukocyte homing. Thus, prevents neutrophil extravasation and thereby protects P.aeruginosa from neutrophil attack. Is also able to inhibit the decay accelerating factor (CD55), but not the cell-surface receptors CD46 and CD31. KEYWORDS: 3D-structure;Hydrolase;Metal-binding;Metalloprotease;Protease;Reference proteome;Secreted;Signal;Zinc SUBCELLULAR LOCATION: Secreted " Q9I8N6,"PROTEIN NAMES: Macrophage colony-stimulating factor 1 receptor (CSF-1 receptor) (CSF-1-R) (CSF-1R) (M-CSF-R) (Proto-oncogene c-Fms homolog) PROTEIN FAMILY: Protein kinase superfamily, Tyr protein kinase family, CSF-1/PDGF receptor subfamily ORGANISM: Danio rerio (Zebrafish) (Brachydanio rerio) SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily. FUNCTION: Tyrosine-protein kinase that acts as a cell-surface receptor for CSF1 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Activates several signaling pathways in response to ligand binding (By similarity). KEYWORDS: ATP-binding;Cell membrane;Disulfide bond;Glycoprotein;Immunity;Immunoglobulin domain;Inflammatory response;Innate immunity;Kinase;Membrane;Nucleotide-binding;Phosphoprotein;Receptor;Reference proteome;Repeat;Signal;Transferase;Transmembrane;Transmembrane helix;Tyrosine-protein kinase;Ubl conjugation SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein Note=The autophosphorylated receptor is ubiquitinated and internalized, leading to its degradation. " Q9IA88,"PROTEIN NAMES: Serine/threonine-protein kinase SIK2 (Qin-induced kinase) (Salt-inducible kinase 2) (SIK-2) (Serine/threonine-protein kinase SNF1-like kinase 2) PROTEIN FAMILY: Protein kinase superfamily, CAMK Ser/Thr protein kinase family, SNF1 subfamily ORGANISM: Gallus gallus (Chicken) SIMILARITY: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily. FUNCTION: Phosphorylates IRS1 in insulin-stimulated adipocytes, potentially modulating the efficiency of insulin signal transduction. Inhibits CREB activity by phosphorylating and repressing the CREB-specific coactivators, CRTC1-3 (By similarity). KEYWORDS: ATP-binding;Cytoplasm;Kinase;Magnesium;Metal-binding;Nucleotide-binding;Phosphoprotein;Reference proteome;Serine/threonine-protein kinase;Transferase SUBCELLULAR LOCATION: Cytoplasm " Q9JHJ8,"PROTEIN NAMES: ICOS ligand (B7 homolog 2) (B7-H2) (B7-like protein Gl50) (B7-related protein 1) (B7RP-1) (LICOS) (CD antigen CD275) PROTEIN FAMILY: Immunoglobulin superfamily, BTN/MOG family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the immunoglobulin superfamily. BTN/MOG family. FUNCTION: Ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells. Could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function. During pregnancy, may function to skew the cytokine of maternal T-cells toward immunoprotective Th2 phenotype. KEYWORDS: Adaptive immunity;Alternative splicing;B-cell activation;Cell membrane;Disulfide bond;Glycoprotein;Immunity;Immunoglobulin domain;Membrane;Reference proteome;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein " Q9JHX4,"PROTEIN NAMES: Caspase-8 (CASP-8) [Cleaved into: Caspase-8 subunit p18; Caspase-8 subunit p10] PROTEIN FAMILY: Peptidase C14A family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the peptidase C14A family. FUNCTION: Thiol protease that plays a key role in programmed cell death by acting as a molecular switch for apoptosis, necroptosis and pyroptosis, and is required to prevent tissue damage during embryonic development and adulthood (By similarity). Initiator protease that induces extrinsic apoptosis by mediating cleavage and activation of effector caspases responsible for FAS/CD95-mediated and TNFRSF1A-induced cell death. Cleaves and activates effector caspases CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10 (By similarity). Binding to the adapter molecule FADD recruits it to either receptor FAS/CD95 or TNFRSF1A. The resulting aggregate called the death-inducing signaling complex (DISC) performs CASP8 proteolytic activation (By similarity). The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases (By similarity). Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC (By similarity). In addition to extrinsic apoptosis, also acts as a negative regulator of necroptosis: acts by cleaving RIPK1 at 'Asp-325', which is crucial to inhibit RIPK1 kinase activity, limiting TNF-induced apoptosis, necroptosis and inflammatory response (By similarity). Also able to initiate pyroptosis by mediating cleavage and activation of gasdermin-C and -D (GSDMC and GSDMD, respectively): gasdermin cleavage promotes release of the N-terminal moiety that binds to membranes and forms pores, triggering pyroptosis (By similarity). Initiates pyroptosis following inactivation of MAP3K7/TAK1 (By similarity). Also acts as a regulator of innate immunity by mediating cleavage and inactivation of N4BP1 downstream of TLR3 or TLR4, thereby promoting cytokine production (By similarity). May participate in the Granzyme B (GZMB) cell death pathways (By similarity). Cleaves PARP1 and PARP2 (By similarity). KEYWORDS: Apoptosis;Cytoplasm;Hydrolase;Nucleus;Phosphoprotein;Protease;Reference proteome;Repeat;Thiol protease;Zymogen SUBCELLULAR LOCATION: Cytoplasm Nucleus " Q9JI44,"PROTEIN NAMES: DNA methyltransferase 1-associated protein 1 (DNMAP1) (DNMT1-associated protein 1) (MAT1-mediated transcriptional repressor) ORGANISM: Mus musculus (Mouse) FUNCTION: Involved in transcription repression and activation. Its interaction with HDAC2 may provide a mechanism for histone deacetylation in heterochromatin following replication of DNA at late firing origins. Can also repress transcription independently of histone deacetylase activity. May specifically potentiate DAXX-mediated repression of glucocorticoid receptor-dependent transcription. Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Participates in the nuclear localization of URI1 and increases its transcriptional corepressor activity (By similarity). KEYWORDS: Alternative splicing;Chromatin regulator;Coiled coil;Cytoplasm;Growth regulation;Isopeptide bond;Nucleus;Phosphoprotein;Reference proteome;Repressor;Transcription;Transcription regulation;Ubl conjugation SUBCELLULAR LOCATION: Nucleus. Cytoplasm Note=Targeted to replication foci throughout S phase by DNMT1. MISCELLANEOUS: [Isoform 2]: May be due to intron retention." Q9JI76,PROTEIN NAMES: Disintegrin and metalloproteinase domain-containing protein 21 (ADAM 21) (Disintegrin and metalloproteinase domain-containing protein 31) (ADAM 31) ORGANISM: Mus musculus (Mouse) FUNCTION: May be involved in sperm maturation and/or fertilization. May also be involved in epithelia functions associated with establishing and maintaining gradients of ions or nutrients. KEYWORDS: Disulfide bond;EGF-like domain;Glycoprotein;Hydrolase;Membrane;Metal-binding;Metalloprotease;Protease;Reference proteome;Signal;Transmembrane;Transmembrane helix;Zinc;Zymogen SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. Q9JI90,"PROTEIN NAMES: E3 ubiquitin-protein ligase RNF14 (RING finger protein 14) PROTEIN FAMILY: RBR family, RNF14 subfamily ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the RBR family. RNF14 subfamily. FUNCTION: E3 ubiquitin-protein ligase that plays a key role in the RNF14-RNF25 translation quality control pathway, a pathway that takes place when a ribosome has stalled during translation, and which promotes ubiquitination and degradation of translation factors on stalled ribosomes (By similarity). Recruited to stalled ribosomes by the ribosome collision sensor GCN1 and mediates 'Lys-6'-linked ubiquitination of target proteins, leading to their degradation (By similarity). Mediates ubiquitination of EEF1A1/eEF1A and ETF1/eRF1 translation factors on stalled ribosomes, leading to their degradation (By similarity). Also catalyzes ubiquitination of ribosomal proteins RPL0, RPL1, RPL12, RPS13 and RPS17 (By similarity). Specifically required to resolve RNA-protein cross-links caused by reactive aldehydes, which trigger translation stress by stalling ribosomes: acts by catalying 'Lys-6'-linked ubiquitination of RNA-protein cross-links, leading to their removal by the ATP-dependent unfoldase VCP and subsequent degradation by the proteasome (By similarity). Independently of its function in the response to stalled ribosomes, acts as a regulator of transcription in Wnt signaling via its interaction with TCF transcription factors (TCF7/TCF1, TCF7L1/TCF3 and TCF7L2/TCF4) (By similarity). May also play a role as a coactivator for androgen- and, to a lesser extent, progesterone-dependent transcription. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Alternative splicing;Cytoplasm;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Repeat;Transcription;Transcription regulation;Transferase;Ubl conjugation;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cytoplasm Nucleus " Q9JJ48,"PROTEIN NAMES: Zinc finger CCCH domain-containing protein 8 (Fetal liver zinc finger protein 1) ORGANISM: Mus musculus (Mouse) FUNCTION: Component of the little elongation complex (LEC), a complex required to regulate small nuclear RNA (snRNA) gene transcription by RNA polymerase II and III. Acts as a transcriptional repressor of the GATA3 promoter. Induces thymocyte apoptosis when overexpressed, which may indicate a role in regulation of thymocyte homeostasis (By similarity). Sequence-specific DNA-binding factor that binds to the 5'-AGGTCTC-3' sequence within the negative cis-acting element intronic regulatory region (IRR) of the GATA3 gene. KEYWORDS: Apoptosis;Isopeptide bond;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Repeat;Repressor;RNA-binding;Transcription;Transcription regulation;Ubl conjugation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Note=Colocalizes with coilin in subnuclear cajal and histone locus bodies. Translocates in the LEC complex to cajal and histone locus bodies at snRNA genes in a ICE1-dependent manner. Associates to transcriptionally active chromatin at snRNA genes (By similarity). " Q9JJF9,"PROTEIN NAMES: Signal peptide peptidase-like 2A (SPP-like 2A) (SPPL2a) (Intramembrane protease 3) (IMP-3) (Presenilin-like protein 2) PROTEIN FAMILY: Peptidase A22B family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the peptidase A22B family. FUNCTION: Intramembrane-cleaving aspartic protease (I-CLiP) that cleaves type II membrane signal peptides in the hydrophobic plane of the membrane. Functions in FASLG, ITM2B and TNF processing. Catalyzes the intramembrane cleavage of the anchored fragment of shed TNF-alpha (TNF), which promotes the release of the intracellular domain (ICD) for signaling to the nucleus. Also responsible for the intramembrane cleavage of Fas antigen ligand FASLG, which promotes the release of the intracellular FasL domain (FasL ICD). Essential for degradation of the invariant chain CD74 that plays a central role in the function of antigen-presenting cells in the immune system. Plays a role in the regulation of innate and adaptive immunity. KEYWORDS: Endosome;Glycoprotein;Hydrolase;Lysosome;Membrane;Protease;Reference proteome;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Late endosome membrane ; Multi-pass membrane protein Lysosome membrane ; Multi-pass membrane protein Membrane ; Multi-pass membrane protein ; Lumenal side Note=Colocalizes with palmitoylated and myristoylated proteins at the plasma membrane. " Q9JJZ6,"PROTEIN NAMES: Krueppel-like factor 13 (Basic transcription element-binding protein 3) (BTE-binding protein 3) (Erythroid transcription factor FKLF-2) (RANTES factor of late activated T-lymphocytes 1) (RFLAT-1) (Transcription factor BTEB3) PROTEIN FAMILY: Sp1 C2H2-type zinc-finger protein family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the Sp1 C2H2-type zinc-finger protein family. FUNCTION: Transcription factor that activates expression from GC-rich minimal promoter regions, including genes in the cells of the erythroid lineage. Represses transcription by binding to the BTE site, a GC-rich DNA element, in competition with the activator SP1. It also represses transcription by interacting with the corepressor Sin3A and HDAC1. Activates RANTES and CCL5 expression in T-cells (By similarity). KEYWORDS: Activator;DNA-binding;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Repeat;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus " Q9JL16,"PROTEIN NAMES: Interferon-stimulated gene 20 kDa protein (Promyelocytic leukemia nuclear body-associated protein ISG20) (Protein DnaQL) PROTEIN FAMILY: Exonuclease superfamily ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the exonuclease superfamily. FUNCTION: Interferon-induced antiviral exoribonuclease that acts mainly on single-stranded RNA. Inhibition of several viruses does not involve the degradation of viral RNAs, but rather the inhibition of translation of viral proteins. Exerts a translational control over a large panel of non-self RNA substrates while sparing endogenous transcripts. This activity correlates with the protein's ability to localize in cytoplasmic processing bodies. May also act as master regulator of over hundred interferon stimulated genes leading to viral genome translation inhibition. May play additional roles in the maturation of snRNAs and rRNAs, and in ribosome biogenesis (By similarity). KEYWORDS: Alternative splicing;Antiviral defense;Cytoplasm;Exonuclease;Hydrolase;Immunity;Innate immunity;Manganese;Metal-binding;Nuclease;Nucleus;Reference proteome;RNA-binding;rRNA processing SUBCELLULAR LOCATION: Nucleus Nucleus, nucleolus Cytoplasm Nucleus, Cajal body Cytoplasm, P-body " Q9JL99,"PROTEIN NAMES: C-type lectin domain family 1 member B (C-type lectin-like receptor 2) (CLEC-2) ORGANISM: Mus musculus (Mouse) FUNCTION: C-type lectin-like receptor that functions as a platelet receptor for the lymphatic endothelial marker, PDPN. After ligand activation, signals via sequential activation of SRC and SYK tyrosine kinases leading to activation of PLCG2. KEYWORDS: Alternative splicing;Disulfide bond;Glycoprotein;Lectin;Membrane;Phosphoprotein;Receptor;Reference proteome;Signal-anchor;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Single-pass type II membrane protein MISCELLANEOUS: Acts as a receptor for the platelet-aggregating snake venom protein rhodocytin. Rhodocytin binding leads to tyrosine phosphorylation and this promotes the binding of spleen tyrosine kinase (Syk) and initiation of downstream tyrosine phosphorylation events and activation of PLC-gamma-2." Q9JLF1,"PROTEIN NAMES: Gamma-aminobutyric acid receptor subunit theta (GABA(A) receptor subunit theta) (GABAAR subunit theta) PROTEIN FAMILY: Ligand-gated ion channel family, Gamma-aminobutyric acid receptor subfamily, GABRQ sub-subfamily ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRQ sub-subfamily. FUNCTION: Theta subunit of the heteropentameric ligand-gated chloride channel gated by gamma-aminobutyric acid (GABA), a major inhibitory neurotransmitter in the brain (By similarity). GABA-gated chloride channels, also named GABA(A) receptors (GABAAR), consist of five subunits arranged around a central pore and contain GABA active binding site(s) located at the alpha and beta subunit interfaces (By similarity). When activated by GABA, GABAARs selectively allow the flow of chloride anions across the cell membrane down their electrochemical gradient (By similarity). KEYWORDS: Cell membrane;Chloride;Chloride channel;Disulfide bond;Glycoprotein;Ion channel;Ion transport;Membrane;Postsynaptic cell membrane;Reference proteome;Signal;Synapse;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Postsynaptic cell membrane; Multi-pass membrane protein Cell membrane; Multi-pass membrane protein " Q9JLF7,"PROTEIN NAMES: Toll-like receptor 5 PROTEIN FAMILY: Toll-like receptor family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the Toll-like receptor family. FUNCTION: Pattern recognition receptor (PRR) located on the cell surface that participates in the activation of innate immunity and inflammatory response. Recognizes small molecular motifs named pathogen-associated molecular pattern (PAMPs) expressed by pathogens and microbe-associated molecular patterns (MAMPs) usually expressed by resident microbiota. Upon ligand binding such as bacterial flagellins, recruits intracellular adapter proteins MYD88 and TRIF leading to NF-kappa-B activation, cytokine secretion and induction of the inflammatory response. Plays thereby an important role in the relationship between the intestinal epithelium and enteric microbes and contributes to the gut microbiota composition throughout life. KEYWORDS: Disulfide bond;Glycoprotein;Immunity;Inflammatory response;Innate immunity;Leucine-rich repeat;Membrane;Phosphoprotein;Receptor;Reference proteome;Repeat;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Single-pass type I membrane protein " Q9JLN5,"PROTEIN NAMES: Erythroid membrane-associated protein PROTEIN FAMILY: Immunoglobulin superfamily, BTN/MOG family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the immunoglobulin superfamily. BTN/MOG family. FUNCTION: Possible role as a cell-adhesion or receptor molecule of erythroid cells. KEYWORDS: Alternative splicing;Cell membrane;Cytoplasm;Disulfide bond;Glycoprotein;Immunoglobulin domain;Membrane;Phosphoprotein;Reference proteome;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein Cytoplasm " Q9JLV6,"PROTEIN NAMES: Bifunctional polynucleotide phosphatase/kinase (DNA 5'-kinase/3'-phosphatase) (Polynucleotide kinase-3'-phosphatase) [Includes: Polynucleotide 3'-phosphatase (2'(3')-polynucleotidase); Polynucleotide 5'-hydroxyl-kinase ] PROTEIN FAMILY: DNA 3' phosphatase family ORGANISM: Mus musculus (Mouse) SIMILARITY: In the N-terminal section; belongs to the DNA 3' phosphatase family. FUNCTION: Plays a key role in the repair of DNA damage, functioning as part of both the non-homologous end-joining (NHEJ) and base excision repair (BER) pathways. Through its two catalytic activities, PNK ensures that DNA termini are compatible with extension and ligation by either removing 3'-phosphates from, or by phosphorylating 5'-hydroxyl groups on, the ribose sugar of the DNA backbone. KEYWORDS: 3D-structure;Acetylation;Alternative splicing;ATP-binding;DNA damage;DNA repair;Hydrolase;Kinase;Multifunctional enzyme;Nucleotide-binding;Nucleus;Phosphoprotein;Reference proteome;Transferase SUBCELLULAR LOCATION: Nucleus " Q9JM58,"PROTEIN NAMES: Cytokine receptor-like factor 1 (Cytokine receptor-like molecule 3) (CRLM-3) (Cytokine-like factor 1) (CLF-1) (Novel cytokine receptor 6) (NR6) PROTEIN FAMILY: Type I cytokine receptor family, Type 3 subfamily ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the type I cytokine receptor family. Type 3 subfamily. FUNCTION: In complex with CLCF1, forms a heterodimeric neurotropic cytokine that plays a crucial role during neuronal development (By similarity). Plays a role in the initiation and/or maintenance of suckling in neonatal mice. May also play a regulatory role in the immune system (By similarity). KEYWORDS: Disulfide bond;Glycoprotein;Immunoglobulin domain;Phosphoprotein;Receptor;Reference proteome;Repeat;Secreted;Signal SUBCELLULAR LOCATION: Secreted " Q9JM90,PROTEIN NAMES: Signal-transducing adaptor protein 1 (STAP-1) (Stem cell adaptor protein 1) ORGANISM: Mus musculus (Mouse) FUNCTION: May function as an adapter molecule downstream of KIT in the proliferation or differentiation of hematopoietic stem cells. KEYWORDS: Alternative splicing;Cytoplasm;Mitochondrion;Nucleus;Phosphoprotein;Reference proteome;SH2 domain SUBCELLULAR LOCATION: Nucleus Cytoplasm Mitochondrion Q9JMD0,"PROTEIN NAMES: BUB3-interacting and GLEBS motif-containing protein ZNF207 (BuGZ) (49 kDa zinc finger protein) (Zinc finger protein 207) ORGANISM: Mus musculus (Mouse) FUNCTION: Kinetochore- and microtubule-binding protein that plays a key role in spindle assembly. ZNF207/BuGZ is mainly composed of disordered low-complexity regions and undergoes phase transition or coacervation to form temperature-dependent liquid droplets. Coacervation promotes microtubule bundling and concentrates tubulin, promoting microtubule polymerization and assembly of spindle and spindle matrix by concentrating its building blocks. Also acts as a regulator of mitotic chromosome alignment by mediating the stability and kinetochore loading of BUB3. Mechanisms by which BUB3 is protected are unclear: according to a first report, ZNF207/BuGZ may act by blocking ubiquitination and proteasomal degradation of BUB3. According to another report, the stabilization is independent of the proteasome (By similarity). KEYWORDS: Alternative splicing;Cell cycle;Cell division;Centromere;Chromosome;Chromosome partition;Cytoplasm;Cytoskeleton;Kinetochore;Metal-binding;Microtubule;Mitosis;Nucleus;Reference proteome;Repeat;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Chromosome, centromere, kinetochore Cytoplasm, cytoskeleton, spindle Note=Localizes primarily to the nucleus in interphase, concentrates at kinetochores prior to nuclear envelope breakdown and during early prometaphase, and disappears from kinetochores upon microtubule-binding. " Q9K0V0,"PROTEIN NAMES: Transferrin-binding protein B (TbpB) (Surface lipoprotein TbpB) (Transferrin-binding protein 2) (TBP-2) PROTEIN FAMILY: TbpB family, Isotype II subfamily ORGANISM: Neisseria meningitidis serogroup B (strain MC58) SIMILARITY: Belongs to the TbpB family. Isotype II subfamily. FUNCTION: Neisseria acquires iron by extracting it from serum transferrin (TF) in its human host. Acts as a TF receptor and is required for TF utilization. Involved in the initial capture of TF. Helps select only those TF molecules that can be used as an iron source and concentrates them on the cell surface, maintaining the iron-loaded status of the TF C-terminal lobe until its delivery to TbpA. KEYWORDS: 3D-structure;Cell outer membrane;Lipoprotein;Membrane;Palmitate;Receptor;Reference proteome;Signal;Virulence SUBCELLULAR LOCATION: Cell outer membrane ; Lipid-anchor Cell surface Note=Requires Slam1 for surface expression in E.coli. MISCELLANEOUS: N.meningitidis cells will only bind to human TF, not bovine or porcine TF, explaining at least in part the bacteria's inability to cause infection in non-human hosts." Q9K498,"PROTEIN NAMES: Bifunctional albaflavenone monooxygenase/terpene synthase (Cytochrome P450 170A1) (CYP170A1) [Includes: Epi-isozizaene 5-monooxygenase ; Beta-farnesene synthase ] PROTEIN FAMILY: Cytochrome P450 family ORGANISM: Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) SIMILARITY: Belongs to the cytochrome P450 family. FUNCTION: Involved in the biosynthesis of the sesquiterpenoid antibiotic albaflavenone. Catalyzes the two-step allylic oxidation of epi-isozizaene to albaflavenone. First carries out a non-stereo-specific oxidation of epi-isozizaene to give a mixture of the albaflavenol epimers ((5R)-albaflavenol and (5S)-albaflavenol), each of which can serve as substrate for the second oxidation to yield albaflavenone. This is quite different from most other P450s which catalyze regio- and stereospecific oxidation. In addition, displays a farnesene synthase activity with farnesyl diphosphate (FPP) as substrate. KEYWORDS: 3D-structure;Heme;Iron;Lyase;Magnesium;Metal-binding;Monooxygenase;Multifunctional enzyme;NADP;Oxidoreductase;Reference proteome MISCELLANEOUS: Both enzymatic and structural data demonstrate that CYP170A1 is a rare bifunctional enzyme having two distinct active sites within a traditional P450 polypeptide chain that individually catalyze two apparently unrelated biochemical reactions." Q9KK62,"PROTEIN NAMES: Bile salt hydrolase/transferase (BSH/T) (Bile acid amine N-acyltransferase) (Bile salt hydrolase) (BSH) (Chenodeoxycholoyltaurine hydrolase) (Choloylglycine hydrolase) (Conjugated bile acid hydrolase) PROTEIN FAMILY: Peptidase C59 family ORGANISM: Bifidobacterium longum SIMILARITY: Belongs to the peptidase C59 family. FUNCTION: Possesses dual functions in bile acid metabolism (By similarity). Acts as a bile salt hydrolase that catalyzes the deconjugation of glycine- and taurine-linked bile salts, which occurs naturally in the intestines of humans, releasing amino acid residues and deconjugated bile salts (bile acids). Can hydrolyze the amide bond in all six major human conjugated bile salts, namely glycocholate (GCA), glycodeoxycholate (GDCA), glycochenodeoxycholate (GCDCA), taurocholate (TCA), taurodeoxycholate (TDCA) and taurochenodeoxycholate (TCDCA). Shows a slight preference for glycine-conjugated bile acids as substrates. Also acts as an amine N-acyltransferase that conjugates a wide variety of amino acids to conjugated and non-conjugated bile acids, thus producing bacterial bile acid amidates (BBAAs) - also named microbially conjugated bile acids (MCBAs) - in the gastrointestinal tract. These BBAAs may facilitate communication between the microbiota and host through the activation of human ligand-activated transcription factors (By similarity). Is totally inactive toward penicillin V. PATHWAY: Lipid metabolism; bile acid biosynthesis. KEYWORDS: 3D-structure;Direct protein sequencing;Hydrolase;Lipid metabolism;Transferase " Q9KLR1,"PROTEIN NAMES: 3'3'-cGAMP-specific phosphodiesterase 1 (3'3'-cGAMP PDE 1) (V-cGAP1) (5'-pApG 5'-nucleotidase) ORGANISM: Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) FUNCTION: Phosphodiesterase (PDE) that catalyzes the hydrolysis of 3'3'-cyclic GMP-AMP (3'3'-cGAMP), leading to linear 5'-pApG. Also displays 5'-nucleotidase activity, further hydrolyzing 5'-pApG to 5'-ApG. Counteracts the function of the 3'3'-cGAMP synthase DncV, and is involved in the modulation of intracellular 3'3'-cGAMP levels. Enhances bacterial chemotaxis and inhibits intestinal colonization in vivo. Thus exerts a crucial role in regulating bacterial infectivity through catalyzing 3'3'-cGAMP degradation. Is specific for 3'3'-cGAMP since it cannot degrade other cGAMP linkage isomers (3'2'-, 2'3'-, and 2'2'-cGAMPs). Is also able to hydrolyze c-di-GMP but not c-di-AMP. KEYWORDS: Calcium;Hydrolase;Magnesium;Metal-binding;Reference proteome " Q9KVG7,"PROTEIN NAMES: Cyclic GMP-AMP synthase (c-GAMP synthase) (cGAS) (3',3'-cGAMP synthase) (Cyclic AMP-GMP synthase) (c-AMP-GMP synthase) (Dinucleotide cyclase DncV) (CD-NTase) PROTEIN FAMILY: CD-NTase family, A01 subfamily ORGANISM: Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) SIMILARITY: Belongs to the CD-NTase family. A01 subfamily. FUNCTION: Cyclic nucleotide synthase (second messenger synthase) of a CBASS antivirus system. CBASS (cyclic oligonucleotide-based antiphage signaling system) provides immunity against bacteriophages. The CD-NTase protein (DncV, this protein) synthesizes cyclic nucleotides in response to infection; these serve as specific second messenger signals. The signals activate a diverse range of effectors, leading to bacterial cell death and thus abortive phage infection. A type II-A(GA) CBASS system.; FUNCTION: Catalyzes the synthesis of 3',3'-cyclic GMP-AMP (cGAMP), a second messenger in cell signal transduction, from GTP and ATP in response to phage infection. Also able to produce c-di-AMP and c-di-GMP from ATP and GTP, respectively; however, cGAMP is the dominant molecule produced by DncV in vivo, contrary to the 2'3'-cGAMP produced by eukaryotes. Is required for efficient V.cholerae intestinal colonization, and down-regulates the colonization-influencing process of chemotaxis. Is not active with dATP, TTP, UTP or CTP. Its product controls the activity of cGAMP-activated phospholipase CapV, a patatin-like lipase that is a direct cGAMP receptor encoded in the dncV operon.; FUNCTION: Protects E.coli against phage infection. When the CBASS operon (capV-dncV-cap2-cap3) is introduced in E.coli MG1655 there is about 100-fold protection against phages P1 and T2. When the operon is introduced in E.coli MG1655 there is a more than 10(3) decrease in the efficiency of T2 plaque formation. Protects 100-fold against phage T5, offers no protection against T7. When the operon is introduced in E.coli MG1655 it protects against phages T2, T4, T5 and T6. Another paper shows the operon confers protection against phages P1, T2, T5 and T6 but not T4 or lambda. KEYWORDS: 3D-structure;Antiviral defense;ATP-binding;GTP-binding;Isopeptide bond;Magnesium;Metal-binding;Nucleotide metabolism;Nucleotide-binding;Nucleotidyltransferase;Reference proteome;Transferase;Virulence MISCELLANEOUS: Overproduction of the cGAMP synthase DncV leads to an increase in intracellular cGAMP, and induces the formation of cells more translucent than normal with a defect in cell membrane integrity, planktonic growth arrest and a small colony phenotype in El Tor V.cholerae." Q9LCV9,"PROTEIN NAMES: N(2)-(2-carboxyethyl)arginine synthase (CEA synthetase) (CEAS) PROTEIN FAMILY: TPP enzyme family ORGANISM: Streptomyces clavuligerus SIMILARITY: Belongs to the TPP enzyme family. FUNCTION: Involved in the biosynthesis of the beta-lactamase inhibitor, clavulanic acid. Catalyzes the thiamine diphosphate (ThDP) dependent condensation of D-glyceraldehyde-3-phosphate (D-G3P) with L-arginine to yield the beta-amino acid, N2-(2-carboxyethyl)arginine (CEA) via a beta-elimination resulting in the formation of an enol which undergoes a second elimination to generate the alpha,beta-unsaturated acryloyl-ThDP. KEYWORDS: 3D-structure;Magnesium;Metal-binding;Thiamine pyrophosphate;Transferase " Q9LD44,"PROTEIN NAMES: NAC transcription factor 56 (AtNAC056) (NAC domain-containing protein 2) (AtNAC2) (Protein NAC-REGULATED SEED MORPHOLOGY 1) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Transcription factor of the NAC family (Probable). Together with NAC018/NARS2, regulates embryogenesis by regulating the development and degeneration of ovule integuments, a process required for intertissue communication between the embryo and the maternal integument. KEYWORDS: DNA-binding;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " Q9LD47,"PROTEIN NAMES: Copper chaperone for superoxide dismutase, chloroplastic/cytosolic (AtCCS) (Superoxide dismutase copper chaperone) PROTEIN FAMILY: Cu-Zn superoxide dismutase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: In the C-terminal section; belongs to the Cu-Zn superoxide dismutase family. FUNCTION: Copper chaperone for the superoxide dismutases CSD1, CSD2 and CSD3. Binds copper ions and delivers them specifically to CSDs. Is required for assistance in CSDs disulfide bond formation and thereby activation of CSDs. May be involved in the negative regulation of heat stress-responsive genes and thermotolerance. KEYWORDS: Alternative splicing;Chaperone;Chloroplast;Copper;Cytoplasm;Metal-binding;Plastid;Reference proteome;Stress response;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast Cytoplasm, cytosol Note=It is unclear whether the chloroplastic and cytosolic proteins result from 2 different transcripts or from alternative translation initiation from the same transcript. " Q9LE33,"PROTEIN NAMES: Glyoxylate/hydroxypyruvate reductase HPR3 (NAD(P)H-dependent hydroxypyruvate reductase 3) (AtHPR3) (HPR 3) PROTEIN FAMILY: D-isomer specific 2-hydroxyacid dehydrogenase family, GyaR subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GyaR subfamily. FUNCTION: Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate (HP) into glycolate and glycerate. Mostly active in the presence of NADPH and glyoxylate. KEYWORDS: Glycolate pathway;NAD;NADP;Oxidoreductase;Photorespiration;Pyruvate;Reference proteome " Q9LE89,"PROTEIN NAMES: Chlorophyllase type 0 (CaCLH0) (Chlorophyll-chlorophyllido hydrolase 0) (Chlase 0) PROTEIN FAMILY: AB hydrolase superfamily, Lipase family ORGANISM: Chenopodium album (Fat hen) SIMILARITY: Belongs to the AB hydrolase superfamily. Lipase family. FUNCTION: Catalyzes the hydrolysis of ester bond in chlorophyll to yield chlorophyllide and phytol. PATHWAY: Porphyrin-containing compound metabolism; chlorophyll degradation. KEYWORDS: Chlorophyll catabolism;Direct protein sequencing;Glycoprotein;Hydrolase;Signal MISCELLANEOUS: It has been proposed that CaCLH0 is transported to vacuole via the endoplasmic reticulum where it might be glycosylated." Q9LEX1,PROTEIN NAMES: Calcium-dependent lipid-binding protein (AtCLB) (CaLB protein) (Protein NTMC 2 TYPE 4) (NTMC2TYPE4) PROTEIN FAMILY: Synaptotagmin family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the synaptotagmin family. FUNCTION: May be involved in membrane trafficking (By similarity). Acts as a repressor of abiotic stress (e.g. drought and salt) responses by binding specifically to the promoter of THAS1 to regulate its transcription. Binds to membrane lipid ceramides. KEYWORDS: Calcium;Coiled coil;Lipid transport;Lipid-binding;Membrane;Metal-binding;Nucleus;Reference proteome;Repeat;Repressor;Stress response;Transcription;Transcription regulation;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Nucleus membrane ; Single-pass membrane protein Note=Localized in the nucleus membrane of root tips cells. Q9LHF5,"PROTEIN NAMES: VIN3-like protein 1 (Protein VERNALIZATION 5) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Involved in both the vernalization and photoperiod pathways by regulating expression of the related floral repressors FLOWERING LOCUS C (FLC) and FLOWERING LOCUS M (FLM). Together with VIN3, required during vernalization for the modifications of FLC and FLM chromatin that are associated with an epigenetically silenced state (e.g. chromatin modifications, histone deacetylation, and trimethylated H3 'Lys-4' H3K4me3 and 'Lys-27' H3K27me3) and with acquisition of competence to flower. Promotes flowering in short days (SD=8 hours light/16 hours dark). Associates dynamically at FLC locus; during vernalization, binds to specific sites, but when in warm conditions, distributed along the whole locus. KEYWORDS: Flowering;Metal-binding;Nucleus;Reference proteome;Repressor;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Nucleus speckle Note=Probably DNA-associated. " Q9LHG8,"PROTEIN NAMES: Protein ELC (AtELC) (ESCRT-I complex subunit VPS23 homolog 1) (Protein VACUOLAR PROTEIN SORTING 23A) (Vacuolar protein-sorting-associated protein 23 homolog 1) PROTEIN FAMILY: Ubiquitin-conjugating enzyme family, UEV subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the ubiquitin-conjugating enzyme family. UEV subfamily. FUNCTION: Component of the ESCRT-I complex (endosomal sorting complex required for transport I), a regulator of vesicular trafficking process. Required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). May control nuclear division through the microtubule cytoskeleton. KEYWORDS: Coiled coil;Endosome;Protein transport;Reference proteome;Transport;Ubl conjugation SUBCELLULAR LOCATION: Early endosome Late endosome Prevacuolar compartment " Q9LIE5,"PROTEIN NAMES: Protein FAR-RED ELONGATED HYPOCOTYL 3 PROTEIN FAMILY: FHY3/FAR1 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the FHY3/FAR1 family. FUNCTION: Transcription activator that recognizes and binds to the DNA consensus sequence 5'-CACGCGC-3'. Activates the expression of FHY1 and FHL involved in light responses. When associated with PHYA, protects it from being recognized and degraded by the COP1/SPA complex. Positive regulator of chlorophyll biosynthesis via the activation of HEMB1 gene expression. KEYWORDS: Metal-binding;Nucleus;Phytochrome signaling pathway;Reference proteome;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus " Q9LIN3,PROTEIN NAMES: Glycine-rich RNA-binding protein RZ1A (AtRZ-1a) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Binds RNA and DNA sequences with a preference to single-stranded nucleic acids. Displays strong affinity to poly(G) and poly(U) sequences. May be involved in tolerance to cold stress. KEYWORDS: Acetylation;Cytoplasm;DNA-binding;Metal-binding;Nucleus;Reference proteome;RNA-binding;Stress response;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Cytoplasm MISCELLANEOUS: Plants over-expressing RZ1A have enhanced freezing tolerance. Q9LIQ6,"PROTEIN NAMES: PHD finger protein ING1 (Protein INHIBITOR OF GROWTH 1) (Protein AtING1) PROTEIN FAMILY: ING family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the ING family. FUNCTION: Histone-binding component that specifically recognizes H3 tails trimethylated on 'Lys-4' (H3K4me3), which mark transcription start sites of virtually all active genes. KEYWORDS: Chromatin regulator;Growth regulation;Metal-binding;Nucleus;Reference proteome;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus " Q9LIR4,"PROTEIN NAMES: Dihydroxy-acid dehydratase, chloroplastic (AthDHAD) (DAD) PROTEIN FAMILY: IlvD/Edd family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the IlvD/Edd family. FUNCTION: Functions in the biosynthesis of branched-chain amino acids. Catalyzes the dehydration of (2R,3R)-2,3-dihydroxy-3-methylpentanoate (2,3-dihydroxy-3-methylvalerate) into 2-oxo-3-methylpentanoate (2-oxo-3-methylvalerate) and of (2R)-2,3-dihydroxy-3-methylbutanoate (2,3-dihydroxyisovalerate) into 2-oxo-3-methylbutanoate (2-oxoisovalerate), the penultimate precursor to L-isoleucine and L-valine, respectively. PATHWAY: Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4.; PATHWAY: Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. KEYWORDS: 2Fe-2S;3D-structure;Acetylation;Alternative splicing;Amino-acid biosynthesis;Branched-chain amino acid biosynthesis;Chloroplast;Iron;Iron-sulfur;Lyase;Magnesium;Metal-binding;Plastid;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast " Q9LJ87,PROTEIN NAMES: Protein CDC73 homolog (Protein PLANT HOMOLOGOUS TO PARAFIBROMIN) PROTEIN FAMILY: CDC73 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the CDC73 family. FUNCTION: Component of the PAF1 complex (PAF1C) which is involved in histone modifications such as methylation on histone H3 'Lys-4' (H3K4me3). Involved in regulation of flowering time. Required for the expression of the flowering repressors FLC and MADS-box genes of the MAF family. Required for histone H3 trimethylation on 'Lys-4' (H3K4me3) at the FLC locus. Prevents trimethylation on 'Lys-27' (H3K27me3) at the same locus. KEYWORDS: Flowering;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus Q9LJB7,"PROTEIN NAMES: B-box zinc finger protein 32 (EIP6, EMF1-INTERACTING PROTEIN 6) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Repressor of light-mediated regulation of seedling development. Functions by suppressing the activities of positive cofactors like BBX21 and HY5 involved in modulating light-regulated gene expression and growth. KEYWORDS: Metal-binding;Nucleus;Reference proteome;Repressor;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus " Q9LJE2,"PROTEIN NAMES: Lysine--tRNA ligase, chloroplastic/mitochondrial (Lysyl-tRNA synthetase) (LysRS) (Lysyl-tRNA synthetase 2) (AtKRS-2) (Protein OVULE ABORTION 5) PROTEIN FAMILY: Class-II aminoacyl-tRNA synthetase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family. FUNCTION: Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. KEYWORDS: Aminoacyl-tRNA synthetase;ATP-binding;Calcium;Chloroplast;DNA-binding;Ligase;Metal-binding;Mitochondrion;Nucleotide-binding;Plastid;Protein biosynthesis;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast Mitochondrion " Q9LJK3,"PROTEIN NAMES: Ceramide synthase LOH2 (CS2) (CSI) (Protein LONGEVITY ASSURANCE GENE ONE HOMOLOG 2) (LAG One Homolog 2) (LAG1 homolog 2) (LAG1 longevity assurance homolog 2) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Prevents cell division in root meristems and promotes salicylic acid (SA) production and hypersensitive response (HR). Catalyzes the biosynthesis of ceramide sphingolipids with C(16) fatty acids, structural membrane lipids involved in membrane trafficking (e.g. early endosomes) and cell polarity (e.g. polar auxin transport related proteins); accepts only C16:0 fatty acids, but with a wide range of d18 sphingoid bases, such as sphinganine (d18:0) and palmitoyl-CoA. Mediates resistance to sphinganine-analog mycotoxins (SAMs, e.g. fumonisin B(1)) by restoring the sphingolipid biosynthesis. Could salvage the transport of GPI-anchored proteins from the endoplasmic reticulum to the Golgi apparatus in ceramides-depleted cells after SAM exposure (By similarity). Contributes to hypoxic conditions tolerance (e.g. submergences), especially in the dark, by promoting the formation of very-long-chain (VLC) ceramide species (22:1, 24:1 and 26:1) and of VLC unsaturated ceramides, which are modulating CTR1-mediated ethylene signaling leading to endoplasmic reticulum (ER)-to-nucleus translocation of EIN2 and EIN3. PATHWAY: Sphingolipid metabolism. KEYWORDS: Endoplasmic reticulum;Lipid biosynthesis;Lipid metabolism;Membrane;Phosphoprotein;Reference proteome;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein " Q9LJZ5,"PROTEIN NAMES: Ubiquitin-conjugating enzyme E2 19 (E2 ubiquitin-conjugating enzyme 19) (Ubiquitin carrier protein 19) PROTEIN FAMILY: Ubiquitin-conjugating enzyme family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the ubiquitin-conjugating enzyme family. FUNCTION: Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Part of the anaphase-promoting complex (APC). May have a key function during cell cycle and be involved in cyclin B1 degradation. Triggers OR ubiquitination that mediates its subsequent nuclear localization. Involved in the repression of early light-induced proteins (ELIPs, e.g. ELIP1 and ELIP2) expression, probably via OR nuclear relocalization. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Alternative splicing;ATP-binding;Cytoplasm;Nucleotide-binding;Nucleus;Reference proteome;Transferase;Ubl conjugation pathway SUBCELLULAR LOCATION: Cytoplasm Nucleus " Q9LK43,"PROTEIN NAMES: Receptor-like kinase TMK4 (BAK1-associating receptor-like kinase 1) (Leucine-rich repeat receptor-like kinases TMK4) (Transmembrane kinase 4) PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. FUNCTION: Involved in auxin signal transduction and cell expansion and proliferation regulation. May be involved in brassinosteroid-mediated plant growth and development via auxin regulation. May be involved in microspore and pollen development. KEYWORDS: ATP-binding;Disulfide bond;Glycoprotein;Kinase;Leucine-rich repeat;Membrane;Nucleotide-binding;Receptor;Reference proteome;Repeat;Serine/threonine-protein kinase;Signal;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Single-pass membrane protein " Q9LK50,"PROTEIN NAMES: ABC transporter G family member 26 (ABC transporter ABCG.26) (AtABCG26) (Putative white-brown complex homolog protein 27) (AtWBC27) PROTEIN FAMILY: ABC transporter superfamily, ABCG family, Eye pigment precursor importer subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily. FUNCTION: Mediates the transport of sporopollenin precursors (e.g. polyketides) across the tapetum plasma membrane into the anther locule for polymerization on developing microspore walls, thus being required for male fertility and pollen exine formation and patterning prior to tapetum programmed cell death. KEYWORDS: ATP-binding;Cell membrane;Endoplasmic reticulum;Membrane;Nucleotide-binding;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Endoplasmic reticulum membrane ; Multi-pass membrane protein " Q9LK94,"PROTEIN NAMES: Monodehydroascorbate reductase 4, peroxisomal (AtMDAR4) PROTEIN FAMILY: FAD-dependent oxidoreductase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the FAD-dependent oxidoreductase family. FUNCTION: Catalyzes the conversion of monodehydroascorbate to ascorbate, oxidizing NADH in the process. Involved in the detoxification of H(2)O(2) that escapes the peroxisome and causes oxidative damage to oil bodies. KEYWORDS: FAD;Flavoprotein;Membrane;NAD;NADP;Oxidoreductase;Peroxisome;Redox-active center;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Peroxisome membrane ; Multi-pass membrane protein " Q9LK95,"PROTEIN NAMES: Transcription factor MYB21 (Myb homolog 3) (AtMyb3) (Myb-related protein 21) (AtMYB21) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Transcription factor involved in photomorphogenesis in the light. May act downstream of the light receptor network and directly affects transcription of light-induced genes. In darkness, its probable degradation prevent the activation of light-induced genes. Required to activate expression of PAL. Acts redundantly with MYB24 and MYB57 to control stamen filament elongation in the late developed flowers. Contributes with MYB24 to induction of MYB108 by jasmonate. Repressed at the transcript levels by DELLA proteins. KEYWORDS: Activator;DNA-binding;Nucleus;Phytochrome signaling pathway;Reference proteome;Repeat;Transcription;Transcription regulation;Ubl conjugation SUBCELLULAR LOCATION: Nucleus " Q9LKI5,"PROTEIN NAMES: DNA repair endonuclease UVH1 (DNA excision repair protein XP-F homolog) (Ultraviolet hypersensitive 1) (AtRAD1) PROTEIN FAMILY: XPF family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the XPF family. FUNCTION: Seems to be involved in nucleotide excision repair (NER) of damaged DNA (dark repair mechanism). Involved in repair of UV light, and probably oxidative damage. The UVH1/RAD1-ERCC1/RAD10 complex may act as an endonuclease making DNA incision 5' to the lesion site. In vitro, is implicated in double strand breaks (DSBs) repair and is required for homologous recombination in the presence of non-homologous overhangs. May mediate the induction of a DNA-damage sensitive cell-cycle checkpoint during the G2 phase. KEYWORDS: Alternative splicing;DNA damage;DNA excision;DNA recombination;DNA repair;DNA-binding;Endonuclease;Hydrolase;Nuclease;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: [Isoform 2]: Inactive.; MISCELLANEOUS: [Isoform 3]: Inactive." Q9LKL2,"PROTEIN NAMES: Two-component response regulator-like APRR1 (ABI3-interacting protein 1) (Pseudo-response regulator 1) (Timing of CAB expression 1) PROTEIN FAMILY: ARR-like family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the ARR-like family. FUNCTION: Controls photoperiodic flowering response. Component of the circadian clock. Expression of several members of the ARR-like family is controlled by circadian rhythm. The particular coordinated sequential expression of APRR9, APRR7, APRR5, APRR3 and APPR1 result to circadian waves that may be at the basis of the endogenous circadian clock. Positive regulator of CCA1 and LHY expression. KEYWORDS: Biological rhythms;Coiled coil;Flowering;Nucleus;Reference proteome;Transcription;Transcription regulation;Two-component regulatory system SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: Subject of targeted degradation by the 26S proteasome. ZEITLUPE (ADO1/ZTL) is the F-box protein that associates with the SCF (for Skp/Cullin/F-box) E3 ubiquitin ligase that is responsible for marking APRR1/TOC1 for turnover. CUL1 is the functional cullin for the SCF(ZTL) complex. APRR3 binding competitively inhibits the ADO1/ZTL interaction." Q9LMG7,"PROTEIN NAMES: Probable inactive purple acid phosphatase 2 PROTEIN FAMILY: Metallophosphoesterase superfamily, Purple acid phosphatase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the metallophosphoesterase superfamily. Purple acid phosphatase family. KEYWORDS: Glycoprotein;Iron;Metal-binding;Reference proteome;Secreted;Signal;Zinc SUBCELLULAR LOCATION: Secreted " Q9LMN8,"PROTEIN NAMES: Wall-associated receptor kinase 3 PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. FUNCTION: Serine/threonine-protein kinase that may function as a signaling receptor of extracellular matrix component. Binding to pectin may have significance in the control of cell expansion, morphogenesis and development. KEYWORDS: ATP-binding;Calcium;Disulfide bond;EGF-like domain;Glycoprotein;Kinase;Membrane;Nucleotide-binding;Phosphoprotein;Reference proteome;Repeat;Serine/threonine-protein kinase;Signal;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Single-pass type I membrane protein " Q9LNE6,"PROTEIN NAMES: Flavonol 7-O-rhamnosyltransferase (UDP-glycosyltransferase 89C1) (UDP-rhamnose: flavonol 7-O-rhamnosyltransferase) PROTEIN FAMILY: UDP-glycosyltransferase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the UDP-glycosyltransferase family. FUNCTION: Flavonol 7-O-rhamnosyltransferase that catalyzes the transfer of rhamnose from UDP-rhamnose to the 7-OH position of 3-O-glycosylated flavonols, such as kaempferol 3-O-rhamnoside, kaempferol 3-O-glucoside, quercetin 3-O-glucoside, quercetin 3-O-galactoside, quercetin 3-O-rhamnoside and isorhamnetin 3-O-glucoside. Is able to glycosylate the flavonols quercetin and kaempferol to yield quercetin 7-O-rhamnoside and kaempferol 7-O-rhamnoside. Shows a strict specificity for UDP-rhamnose as sugar donor. Does not act on 3-O-glycosylated anthocyanins. The accumulation of kaempferol 3-O-rhamnoside-7-O-rhamnoside inhibits basipetal auxin transport, which influences auxin distribution and plant organ development. PATHWAY: Flavonoid metabolism. KEYWORDS: 3D-structure;Glycosyltransferase;Reference proteome;Transferase " Q9LNW3,"PROTEIN NAMES: Protein phosphatase 2C 3 (AtPP2C03) (Protein AKT1-INTERACTING 1) (Protein HIGHLY ABA-INDUCED PP2C 2) (Protein HONSU) (Protein phosphatase 2C AIP1) (PP2C AIP1) PROTEIN FAMILY: PP2C family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the PP2C family. FUNCTION: Involved in the negative regulation of the K(+) potassium channel AKT1 by its dephosphorylation, antagonistically to CIPK proteins (e.g. CIPK23). Functions as a positive regulator of abscisic acid-mediated cell signaling during seedling growth. Involved in the regulation of seed dormancy. Acts as a negative regulator of seed dormancy by inhibiting abscisic signaling and subsequently activating gibberellic acid signaling. KEYWORDS: Cell membrane;Cytoplasm;Growth regulation;Hydrolase;Magnesium;Manganese;Membrane;Metal-binding;Nucleus;Protein phosphatase;Reference proteome SUBCELLULAR LOCATION: Cell membrane Cytoplasm Nucleus Note=Probably associated to the plasma membrane when interacting with AKT1 and CIPK23 (Probable). Localizes to nucleus when interacting with PYL8/RCAR3. MISCELLANEOUS: Honsu means abnormal drowsiness in Korean." Q9LPT1,"PROTEIN NAMES: Pollen receptor-like kinase 5 (AtPRK5) PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. FUNCTION: Receptor-like kinase involved in the control of pollen germination and pollen tube polar growth. The extracellular domain serves as a sensor for peptides derived from GRI. May act as a downstream element for ROS-dependent cell death induced by GRI. KEYWORDS: ATP-binding;Cell membrane;Glycoprotein;Kinase;Leucine-rich repeat;Membrane;Nucleotide-binding;Phosphoprotein;Receptor;Reference proteome;Repeat;Serine/threonine-protein kinase;Signal;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein " Q9LQ55,"PROTEIN NAMES: Dynamin-2B (Dynamin-like protein 3) (Dynamin-related protein 2B) PROTEIN FAMILY: TRAFAC class dynamin-like GTPase superfamily, Dynamin/Fzo/YdjA family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family. FUNCTION: Putative microtubule-associated force-producing protein, able to bind and hydrolyze GTP (By similarity). Collaboratively with DRP1A, participates in clathrin-coated vesicle formation during endocytosis. With DRP1A and PIP5K3, required for the precise coordination of polar ARAC3/ROP6 and ARAC4/ROP2 placement and subsequent root hair positioning during planar polarity formation in root hair-forming cells. KEYWORDS: Acetylation;Cell membrane;Coiled coil;Cytoplasm;Cytoplasmic vesicle;Cytoskeleton;GTP-binding;Hydrolase;Membrane;Microtubule;Motor protein;Nucleotide-binding;Reference proteome SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton Cytoplasmic vesicle, clathrin-coated vesicle Cell membrane Note=Accumulates in a sterol-enriched, polar membrane domain during root hair initiation. " Q9LQX5,"PROTEIN NAMES: Transcription factor MYB117 (MYB-domain transcription factor LOF1) (Myb-related protein 117) (AtMYB117) (Protein CONSTRICTED FRUIT) (Protein LATERAL ORGAN FUSION 1) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Probable transcription factor that involved in boundary specification, meristem initiation and maintenance, and organ patterning. Functions in both lateral organ separation and axillary meristem formation, in part through genetic interaction with the NAC domain genes CUC2 and CUC3 and the homeobox gene STM. May be recruited by a variety of developmental programs for the development of floral organs and the initiation of ovule outgrowth. KEYWORDS: Alternative splicing;Developmental protein;DNA-binding;Nucleus;Reference proteome;Repeat;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " Q9LQZ5,"PROTEIN NAMES: Protein SHI RELATED SEQUENCE 5 PROTEIN FAMILY: SHI protein family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the SHI protein family. FUNCTION: Transcription activator that binds DNA on 5'-ACTCTAC-3' and promotes auxin homeostasis-regulating gene expression (e.g. YUC genes), as well as genes affecting stamen development, cell expansion and timing of flowering. Synergistically with other SHI-related proteins, regulates gynoecium, stamen and leaf development in a dose-dependent manner, controlling apical-basal patterning. Promotes style and stigma formation, and influences vascular development during gynoecium development. May also have a role in the formation and/or maintenance of the shoot apical meristem (SAM). KEYWORDS: Activator;Auxin biosynthesis;Auxin signaling pathway;Developmental protein;DNA-binding;Metal-binding;Nucleus;Reference proteome;Zinc SUBCELLULAR LOCATION: Nucleus " Q9LQZ7,PROTEIN NAMES: B-box zinc finger protein 21 (AtBBX21) (Protein LONG HYPOCOTYL UNDER SHADE) (Protein SALT TOLERANCE HOMOLOG 2) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Transcription activator that acts as a positive regulator of seedling photomorphogenesis. Acts downstream of COP1 and play an important role in early and long-term adjustment of the shade avoidance syndrome (SAS) responses in natural environments. KEYWORDS: Activator;Metal-binding;Nucleus;Reference proteome;Repeat;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Q9LR44,"PROTEIN NAMES: UDP-glycosyltransferase 75B1 ((Uridine 5'-diphosphate-glucose:indol-3-ylacetyl)-beta-D-glucosyl transferase 1) (IAA-Glu synthase 1) (Indole-3-acetate beta-glucosyltransferase 1) PROTEIN FAMILY: UDP-glycosyltransferase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the UDP-glycosyltransferase family. FUNCTION: Possesses low catalytic activity on indole-3-acetic acid (IAA) in vitro. May transfer UDP-glucose from sucrose synthase to callose synthase for the synthesis of callose at the forming cell plate during cytokinesis. Has high affinity for 4-aminobenzoate. Catalyzes the formation of 4-aminobenzoate glucose ester which represents a storage form of 4-aminobenzoate in the vacuole. Is the major source of this activity in the plant. Also active in vitro on benzoates and benzoate derivatives. PATHWAY: Plant hormone metabolism; auxin conjugation. KEYWORDS: Cytoplasm;Cytoskeleton;Glycosyltransferase;Reference proteome;Transferase SUBCELLULAR LOCATION: Cytoplasm, perinuclear region Cytoplasm, cytoskeleton, phragmoplast Note=During interphase, distributed in a punctate pattern in the perinuclear region. Localized in the forming cell plate during cytokinesis. " Q9LR47,"PROTEIN NAMES: Type IV inositol polyphosphate 5-phosphatase 6 (At5PTase6) (Protein COTYLEDON VASCULAR PATTERN 2) PROTEIN FAMILY: Inositol polyphosphate 5-phosphatase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the inositol polyphosphate 5-phosphatase family. FUNCTION: Has phosphatase activity toward PtdIns(4,5)P2 and PtdIns(3,4,5)P3. Required for the patterning of procambium and during the differentiation of vascular tissues. Acts before the acquisition of preprocambial identity. Seems to be also involved in the abscisic acid (ABA) signaling pathway. Acts redundantly with CVL1 for maintaining vascular continuity. Regulates phosphoinositide-dependent VAN3 localization. KEYWORDS: Abscisic acid signaling pathway;Alternative splicing;Differentiation;Hydrolase;Reference proteome MISCELLANEOUS: CVP2 overexpression impairs protophloeme sieve element differentiation and overall root growth. Cvp1 and cvp2 double mutant displays high PtdIns(4,5)P2 levels.; MISCELLANEOUS: [Isoform 2]: May be due to a competing donor splice site." Q9LRB7,"PROTEIN NAMES: E3 ubiquitin-protein ligase EL5 (Protein ELICITOR 5) (RING-type E3 ubiquitin transferase EL5) ORGANISM: Oryza sativa subsp. japonica (Rice) FUNCTION: Functions as an E3 ubiquitin-protein ligase in cooperation with the E2 ubiquitin conjugating enzymes UBC5A and UBC5B. Involved in root development. Required for the maintenance of cell viability after the initiation of root primordial formation. May mediate the degradation of cytotoxic proteins produced in root cells after the actions of auxin, cytokinin and jasmonic acid. Mediates 'Lys-48'-linked polyubiquitination of MBP in vitro. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: 3D-structure;Cell membrane;Membrane;Metal-binding;Reference proteome;Transferase;Transmembrane;Transmembrane helix;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cell membrane ; Single-pass membrane protein " Q9LRH6,"PROTEIN NAMES: GATA transcription factor 25 (Protein TIFY 1) (Protein ZIM) PROTEIN FAMILY: Type IV zinc-finger family, Class C subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the type IV zinc-finger family. Class C subfamily. FUNCTION: Transcriptional activator that specifically binds 5'-GATA-3' or 5'-GAT-3' motifs within gene promoters. KEYWORDS: Activator;Alternative splicing;DNA-binding;Metal-binding;Nucleus;Reference proteome;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: [Isoform 2]: May be due to intron retention." Q9LS40,PROTEIN NAMES: Protein ASPARTIC PROTEASE IN GUARD CELL 1 (AtASPG1) PROTEIN FAMILY: Peptidase A1 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the peptidase A1 family. FUNCTION: Aspartic protease involved in drought avoidance through abscisic acid signaling. KEYWORDS: Aspartyl protease;Disulfide bond;DNA-binding;Endoplasmic reticulum;Hydrolase;Protease;Reference proteome;Signal SUBCELLULAR LOCATION: Endoplasmic reticulum Q9LSI7,PROTEIN NAMES: Transcription factor MYB35 (Myb-related protein 35) (AtMYB35) (Protein DEFECTIVE IN TAPETAL DEVELOPMENT AND FUNCTION 1) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Required for anther development and early tapetal function during microspore maturation. Regulates callose dissolution required for microspores release from the tetrads. KEYWORDS: Developmental protein;DNA-binding;Nucleus;Reference proteome;Repeat;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus Q9LSI9,"PROTEIN NAMES: Inactive LRR receptor-like serine/threonine-protein kinase BIR2 (Protein BAK1-INTERACTING RECEPTOR-LIKE KINASE 2) PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. FUNCTION: Pseudokinases lacking protein kinase activity and unable to bind ATP-analogs. Negative regulator of pathogen-associated molecular patterns- (PAMP-) triggered immunity by limiting BAK1-receptor complex formation in the absence of ligands. KEYWORDS: 3D-structure;ATP-binding;Cell membrane;Glycoprotein;Leucine-rich repeat;Membrane;Nucleotide-binding;Phosphoprotein;Plant defense;Receptor;Reference proteome;Repeat;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein " Q9LSL5,"PROTEIN NAMES: L-type lectin-domain containing receptor kinase IX.2 (LecRK-IX.2) PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family; Leguminous lectin family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: In the C-terminal section; belongs to the protein kinase superfamily. Ser/Thr protein kinase family.; SIMILARITY: In the N-terminal section; belongs to the leguminous lectin family. FUNCTION: Promotes hydrogen peroxide H(2)O(2) production and cell death.; FUNCTION: Involved in resistance response to the pathogenic oomycetes Phytophthora infestans and Phytophthora capsici. KEYWORDS: ATP-binding;Cell membrane;Glycoprotein;Kinase;Lectin;Membrane;Nucleotide-binding;Plant defense;Receptor;Reference proteome;Serine/threonine-protein kinase;Signal;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein MISCELLANEOUS: Both lectin domain and kinase activity are required for resistance to oomycetes, but only the lectin domain is required to trigger cell death." Q9LSP7,PROTEIN NAMES: Ubiquitin-conjugating enzyme E2 32 (E2 ubiquitin-conjugating enzyme 32) (Ubiquitin carrier protein 32) PROTEIN FAMILY: Ubiquitin-conjugating enzyme family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the ubiquitin-conjugating enzyme family. FUNCTION: Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: ATP-binding;Membrane;Nucleotide-binding;Reference proteome;Transferase;Transmembrane;Transmembrane helix;Ubl conjugation pathway SUBCELLULAR LOCATION: Membrane ; Single-pass membrane protein Q9LTA6,PROTEIN NAMES: E3 ubiquitin-protein ligase WAV3 (Protein WAVY GROWTH 3) (RING-type E3 ubiquitin transferase WAV3) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: E3 ubiquitin-protein ligase involved in the regulation of root growth. Acts as a positive regulator of root gravitropism. Possesses E3 protein ligase activity in vitro. KEYWORDS: Metal-binding;Reference proteome;Transferase;Ubl conjugation pathway;Zinc;Zinc-finger Q9LTJ6,"PROTEIN NAMES: WD repeat-containing protein RUP1 (Protein EARLY FLOWERING BY OVEREXPRESSION 1) (Protein REPRESSOR OF UV-B PHOTOMORPHOGENESIS 1) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Functions in association with RUP2 as repressor of UV-B-induced photomorphogenesis mediated by UVR8 and HY5, likely in coordination with DHU1. Plays a crucial negative feedback regulatory role downstream of UVR8-COP1 to inhibit UVR8 function, balance UV-B-specific responses and ensure normal plant growth. Is involved in the regulation of photoperiodic flowering and vegetative development. KEYWORDS: Cytoplasm;Flowering;Nucleus;Reference proteome;Repeat;WD repeat SUBCELLULAR LOCATION: Nucleus Cytoplasm, cytosol MISCELLANEOUS: Overexpression of RUP1 confers an early flowering phenotype." Q9LTQ0,PROTEIN NAMES: Ribonuclease 3-like protein 2 (Ribonuclease III-like protein 2) (RNase III-like protein 2) (Ribonuclease three-like protein 2) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Ribonuclease that cleaves double-stranded RNA (dsRNA). Required for 3'-external transcribed spacer (ETS) cleavage of the pre-rRNA precursors. May promote the production of 21 nucleotide small interfering RNA (siRNA) during post-transcriptional gene silencing (PTGS). KEYWORDS: Cytoplasm;Disulfide bond;Endonuclease;Hydrolase;Magnesium;Manganese;Metal-binding;Nuclease;Nucleus;Reference proteome;Repeat;RNA-binding;rRNA processing SUBCELLULAR LOCATION: Nucleus Cytoplasm Q9LU36,"PROTEIN NAMES: 4-coumarate--CoA ligase 4 (4CL 4) ((E)-ferulate--CoA ligase) (4-coumarate--CoA ligase isoform 5) (At4CL5) (4-coumaroyl-CoA synthase 4) (Sinapate--CoA ligase) PROTEIN FAMILY: ATP-dependent AMP-binding enzyme family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the ATP-dependent AMP-binding enzyme family. FUNCTION: Produces CoA thioesters of a variety of hydroxy- and methoxy-substituted cinnamic acids, which are used to synthesize several phenylpropanoid-derived compounds, including anthocyanins, flavonoids, isoflavonoids, coumarins, lignin, suberin and wall-bound phenolics. Follows a two-step reaction mechanism, wherein the carboxylate substrate first undergoes adenylation by ATP, followed by a thioesterification in the presence of CoA to yield the final CoA thioesters (By similarity). PATHWAY: Phytoalexin biosynthesis; 3,4',5-trihydroxystilbene biosynthesis; 3,4',5-trihydroxystilbene from trans-4-coumarate: step 1/2. KEYWORDS: ATP-binding;Ligase;Magnesium;Nucleotide-binding;Phenylpropanoid metabolism;Reference proteome MISCELLANEOUS: Activates efficiently sinapate, besides the usual 4CL substrates (4-coumarate, caffeate, and ferulate)." Q9LU39,"PROTEIN NAMES: Glutamyl-tRNA reductase-binding protein, chloroplastic (AtGluTRBP) (GluTR-binding protein) (Protein PROTON GRADIENT REGULATION 7) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Involved in the regulation of glutamyl-tRNA reductase (GluTR) which is important for the synthesis and distribution of 5-aminolevulinate, a precursor in heme and chlorophyll biosynthesis. Stimulates GluTR activity and regulates glutamate-1-semialdehyde release. May play a role in heme metabolism. Necessary for efficient photosynthetic electron transport in chloroplasts. KEYWORDS: 3D-structure;Chlorophyll biosynthesis;Chloroplast;Heme biosynthesis;Plastid;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast stroma Note=Detected at low levels in thylakoids. " Q9LU44,PROTEIN NAMES: Splicing factor-like protein 1 (AtSF1) (SF1 homolog protein) PROTEIN FAMILY: BBP/SF1 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the BBP/SF1 family. FUNCTION: Necessary for the splicing of pre-mRNA. Required during development and for abscisic acid (ABA) responses. KEYWORDS: Abscisic acid signaling pathway;Metal-binding;mRNA processing;mRNA splicing;Nucleus;Phosphoprotein;Reference proteome;Repeat;RNA-binding;Spliceosome;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Q9LU63,"PROTEIN NAMES: Probable pterin-4-alpha-carbinolamine dehydratase, chloroplastic (4-alpha-hydroxy-tetrahydropterin dehydratase) (PCD/DCoH-like protein 1) (Protein AIRP2 TARGET PROTEIN 1) (Protein SDIR1-INTERACTING PROTEIN 1) PROTEIN FAMILY: Pterin-4-alpha-carbinolamine dehydratase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the pterin-4-alpha-carbinolamine dehydratase family. FUNCTION: Involved in tetrahydrobiopterin biosynthesis (By similarity). Interacts with and acts downstream of the E3 ubiquitin-protein ligase SDIR1 in abscisic acid (ABA) and salt stress signaling. Regulates the expression of the bZIP transcription factor ABI5, which mediates responses to ABA during seed germination and salt stress. The SDIR1-ATP1/SDIRIP1 complex plays an important role in ABA signaling through the ubiquitination pathway. Acts downstream of AIRP2 in regulation of ABA signaling during drought stress. KEYWORDS: Cell membrane;Chloroplast;Lyase;Membrane;Nucleus;Plastid;Reference proteome;Tetrahydrobiopterin biosynthesis;Transit peptide;Ubl conjugation SUBCELLULAR LOCATION: Plastid, chloroplast Cell membrane ; Peripheral membrane protein Nucleus " Q9LUJ3,"PROTEIN NAMES: Protein RDM1 (Protein RNA-directed DNA methylation 1) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Regulator of RNA-directed DNA methylation (RdDM). Binds to single-stranded methyl DNA. Involved in the assembly of RNA polymerase V (Pol V) transcription initiation or elongation complexes at the chromatin, as a component of the DDR complex. KEYWORDS: 3D-structure;DNA-binding;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus, nucleoplasm Note=Peri-nucleolar. " Q9LUL2,"PROTEIN NAMES: Serine/threonine-protein kinase WAG2 PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. FUNCTION: Serine/threonine-protein kinase involved in the regulation of auxin signaling. Acts as a positive regulator of cellular auxin efflux and regulates organ development by enhancing PIN-mediated polar auxin transport. Phosphorylates conserved serine residues in the PIN auxin efflux carriers. Phosphorylation of PIN proteins is required and sufficient for apical-basal PIN polarity that enables directional intercellular auxin fluxes, which mediate differential growth, tissue patterning and organogenesis. Acts as a suppressor of root waving. KEYWORDS: ATP-binding;Auxin signaling pathway;Cytoplasm;Developmental protein;Growth regulation;Kinase;Nucleotide-binding;Reference proteome;Serine/threonine-protein kinase;Transferase SUBCELLULAR LOCATION: Cytoplasm, cytosol Note=Targeted to the cell periphery. MISCELLANEOUS: Over-expression of WAG1 induces a basal-to-apical shift in PIN1, PIN2 and PIN4 localization, resulting in the loss of auxin gradients and strong defects in embryo and seedling roots." Q9LVA9,"PROTEIN NAMES: Small ribosomal subunit protein mS75 (Protein GAMETE CELL DEFECTIVE 1, mitochondrial) (AtGCD1) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Essential for fertility (male and female gametophyte functions and development). Required for the integrity of female gametic mitochondria. Modulates male gametophyte functions, including pollen tube growth and style penetration. Involved in mitochondrial-driven cell-to-cell communication in embryo sacs during female gametes maturation (including embryogenesis initiation and endosperm development), especially for reciprocal signaling between central and egg cells which regulates reciprocal development. KEYWORDS: 3D-structure;Alternative splicing;Developmental protein;Mitochondrion;Reference proteome;Ribonucleoprotein;Ribosomal protein;Transit peptide SUBCELLULAR LOCATION: Mitochondrion " Q9LVE2,"PROTEIN NAMES: Serine/threonine protein phosphatase 2A 59 kDa regulatory subunit B' zeta isoform (AtB' zeta) (PP2A, B' subunit, zeta isoform) PROTEIN FAMILY: Phosphatase 2A regulatory subunit B56 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the phosphatase 2A regulatory subunit B56 family. FUNCTION: The B regulatory subunit may modulate substrate selectivity and catalytic activity, and may also direct the localization of the catalytic enzyme to a particular subcellular compartment (By similarity). The holoenzyme composed of PP2AA1, PP2A4 and B'ZETA acts as negative regulator of plant innate immunity by controlling BAK1 phosphorylation state and activation in surface-localized immune receptor complexes. Required for the formation of the PP2A holoenzyme that negatively regulates brassinosteroid signaling by dephosphorylating and inactivating BRI1 in the cytoplasm. Involved in growth regulation and stress signaling. Involved in the regulation of reactive oxygen species (ROS) signaling. KEYWORDS: Brassinosteroid signaling pathway;Cytoplasm;Mitochondrion;Plant defense;Reference proteome;Stress response SUBCELLULAR LOCATION: Cytoplasm, cytosol Mitochondrion " Q9LVG2,"PROTEIN NAMES: AP2-like ethylene-responsive transcription factor TOE2 (Protein TARGET OF EAT 2) PROTEIN FAMILY: AP2/ERF transcription factor family, AP2 subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the AP2/ERF transcription factor family. AP2 subfamily. FUNCTION: Probably acts as a transcriptional activator. Binds to the GCC-box pathogenesis-related promoter element. May be involved in the regulation of gene expression by stress factors and by components of stress signal transduction pathways (By similarity). Regulates negatively the transition to flowering time and confers flowering time delay. KEYWORDS: Activator;Alternative splicing;DNA-binding;Ethylene signaling pathway;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " Q9LVG4,"PROTEIN NAMES: Two-component response regulator-like APRR3 (Pseudo-response regulator 3) PROTEIN FAMILY: ARR-like family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the ARR-like family. FUNCTION: Controls photoperiodic flowering response. Component of the circadian clock. Controls the degradation of APRR1/TOC1 by the SCF(ZTL) complex. Expression of several members of the ARR-like family is controlled by circadian rhythm. The particular coordinated sequential expression of APRR9, APRR7, APRR5, APRR3 and APPR1 result to circadian waves that may be at the basis of the endogenous circadian clock. KEYWORDS: Alternative splicing;Biological rhythms;Coiled coil;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation;Two-component regulatory system SUBCELLULAR LOCATION: Nucleus " Q9LVI9,"PROTEIN NAMES: Dihydropyrimidine dehydrogenase (NADP(+)), chloroplastic (DHPDH) (DPD) (Dihydroorotate dehydrogenase-like protein) (Dihydrothymine dehydrogenase) (Dihydrouracil dehydrogenase) (Protein PYRIMIDINE 1) PROTEIN FAMILY: Dihydropyrimidine dehydrogenase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the dihydropyrimidine dehydrogenase family. FUNCTION: Involved in pyrimidine base degradation. Catalyzes the reduction of uracil to 5,6-dihydrouracil (DHU) by using NADH as a specific cosubstrate and the reduction of thymine to 5,6-dihydrothymine (DHT). Involved in the recycling of nitrogen from nucleobases to general nitrogen metabolism. PATHWAY: Amino-acid biosynthesis; beta-alanine biosynthesis. KEYWORDS: Acetylation;Chloroplast;NADP;Oxidoreductase;Plastid;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast " Q9LVQ0,"PROTEIN NAMES: Pectinesterase 31 (PE 31) (Pectin methylesterase 31) (AtPME31) PROTEIN FAMILY: Pectinesterase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the pectinesterase family. FUNCTION: Acts in the modification of cell walls via demethylesterification of cell wall pectin. Acts in a blockwise manner, resulting in a cell wall rigidification. PATHWAY: Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5. KEYWORDS: Aspartyl esterase;Hydrolase;Reference proteome MISCELLANEOUS: This is the only member of the pectinesterase family that do not contain a transmembrane or a signal peptide." Q9LVS0,"PROTEIN NAMES: Transcription factor KUA1 (Myb-related protein H) (AtMYBH) (AtMYBS3) (MYBS3-homolog protein) (Protein KUODA1) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Transcriptional repressor. Direct regulator of the transcription of peroxidase (Prxs) and reactive oxygen species (ROS)-related genes via the recognition of 5'-ATCACA-3' motif. Binds to 5'-TATCCA-3' motif (TA box) and represses the activity of corresponding promoters (e.g. sugar response genes). Regulates hypocotyl elongation in response to darkness by enhancing auxin accumulation in a phytochrome-interacting factor (PIF) proteins-dependent manner. Promotes lateral roots formation. Promotes cell expansion during leaves development via the modulation of cell wall-located Prxs. Plays a critical role in developmentally regulated and dark-induced onset of leaf senescence by repressing the transcription of several genes involved in chloroplast function and responses to light and auxin. Promotes responses to auxin, abscisic acid (ABA), and ethylene. KEYWORDS: DNA-binding;Metal-binding;Nucleus;Reference proteome;Repressor;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: 'Kuoda' means 'enlarge' or 'expand' in Chinese." Q9LVW4,"PROTEIN NAMES: Transcription factor MYB118 (Myb-related protein 118) (AtMYB118) (Protein PLANT GROWTH ACTIVATOR 37) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Transcription activator that recognizes the motif 5'-TAACGG-3' in the promoter of endosperm-induced genes. Promotes vegetative-to-embryonic transition and the formation of somatic embryos from root explants in a WUS-independent manner but via the expression of embryonic genes (e.g. LEC1, LEC2, FUS3 and WUS). May play an important role during embryogenesis and seed maturation. Together with MYB115, activates the transcription of S-ACP-DES2/AAD2 and S-ACP-DES3/AAD3 thus promoting the biosynthesis of omega-7 monounsaturated fatty acid in seed endosperm. Negatively regulates maturation genes in the endosperm. KEYWORDS: Activator;DNA-binding;Nucleus;Reference proteome;Repeat;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " Q9LX14,"PROTEIN NAMES: Protein SOSEKI 2 (AtSOK2) (Protein UPSTREAM OF FLC) PROTEIN FAMILY: SOSEKI family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the SOSEKI family. FUNCTION: Part of a three-gene cluster containing FLC, UFC and DFC, which is coordinately regulated in response to vernalization. Also regulated by FLX. SOSEKI proteins (SOK1-5) locally interpret global polarity cues and can influence cell division orientation to coordinate cell polarization relative to body axes, probably by guiding ANGUSTIFOLIA (AN) polarized localization. KEYWORDS: Cell cycle;Cell division;Cell membrane;Developmental protein;Membrane;Reference proteome SUBCELLULAR LOCATION: Cell membrane ; Peripheral membrane protein ; Cytoplasmic side Note=SOSEKI proteins integrate apical-basal and radial organismal axes to localize to polar cell edges, pointing towards the epidermis, mainly to inner basal edges. MISCELLANEOUS: 'Soseki' means cornerstone in Japanese." Q9LXT4,"PROTEIN NAMES: Plant cysteine oxidase 5 (AtPCO5) PROTEIN FAMILY: Cysteine dioxygenase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the cysteine dioxygenase family. FUNCTION: Catalyzes the oxidation of N-terminal cysteine residues (N-Cys), thus preparing the protein for N-end rule pathway-mediated proteasomal degradation, upstream of the N-end rule enzymes ATE1, ATE2 and PRT6 (Probable). Controls the preparation of the group VII ethylene response factor (ERF-VII) proteins for degradation via the 26S proteasome N-end rule pathway (Probable). Acts as an oxygen sensor that controls the stability of ERF-VII proteins, which are stabilized in flooding-induced hypoxia, and regulate transcriptional adaptation to these adverse conditions (Probable). KEYWORDS: 3D-structure;Cytoplasm;Iron;Metal-binding;Nucleus;Oxidoreductase;Reference proteome SUBCELLULAR LOCATION: Nucleus Cytoplasm " Q9LY00,"PROTEIN NAMES: Probable WRKY transcription factor 70 (WRKY DNA-binding protein 70) PROTEIN FAMILY: WRKY group III family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the WRKY group III family. FUNCTION: Transcription factor involved in senescence, biotic and abiotic stress responses by modulating various phytohormones signaling pathways. Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element (By similarity). Binds to the 5'-[CT]GACTTTT-3' motif in promoters of target genes to induce their expression. Plays an important but not indispensable role in jasmonate and salicylic acid signaling. Positively regulates the salicylic acid (SA)-mediated signal pathway, but negatively the jasmonic acid (JA)-mediated signal pathway, thus determining the balance between these mutually antagonistic pathways. Together with WRKY46, WRKY53 and WRKY54, prevents defense response to the necrotrophic pathogens P.carotovorum and B.cinerea, but promotes defense responses (including SA-induced pathogenesis-related (PR) genes expression) against biotrophic/hemibiotrophic SA-monitored pathogens (e.g. P.syringae, E.carotovora subsp. carotovora SCC3193 and E.cichoracearum), probably by regulating negatively the JA/ET and positively the SA signaling pathways. Contributes to the suppression of jasmonic acid (MeJA)-induced expression of JA-responsive genes (e.g. PDF1.2). Promotes susceptibility to JA-monitored pathogens (e.g. A.brassicicola), probably by facilitating SA-controlled suppression of JA-mediated defense. Represses the biosynthesis of the phytoalexin camalexin and indol-3-ylmethyl glucosinolate (IGS). Represses both SA and JA/ethylene (ET) mediated defense marker genes expression. Negative regulator of SA biosynthesis. Negative regulator of EDS1-dependent defense against E.amylovora. Required for RPP4-mediated disease resistance and basal defense against H.parasitica, probably via late up-regulation (LURP) of resistance genes (e.g. CML10/CaBP22 and LURP1). Probably involved in defense responses toward insects (e.g. P.xylostella and B.brassicae). Together with WRKY54, negative regulator of developmental senescence, probably via the regulation of several senescence-associated markers genes. Together with WRKY46 and WRKY54, promotes brassinosteroid (BR)-regulated plant growth but prevent drought response by modulating gene expression. In collaboration with WRKY54, prevents stomatal closure and, consequently, osmotic stress tolerance. Regulates rhizobacterium B.cereus AR156-induced systemic resistance (ISR) to P.syringae pv. tomato DC3000. KEYWORDS: Brassinosteroid signaling pathway;DNA-binding;Ethylene signaling pathway;Jasmonic acid signaling pathway;Nucleus;Plant defense;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " Q9LY46,"PROTEIN NAMES: Xylan O-acetyltransferase 1 (Protein ESKIMO 1) (Protein trichome birefringence-like 29) PROTEIN FAMILY: PC-esterase family, TBL subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the PC-esterase family. TBL subfamily. FUNCTION: Xylan acetyltransferase required for 2-O- and 3-O-monoacetylation of xylosyl residues in xylan. Catalyzes the 2-O-acetylation of xylan, followed by nonenzymatic acetyl migration to the O-3 position, resulting in products that are monoacetylated at both O-2 and O-3 positions. Is necessary for the formation of the functional xylem, which is required for water transport to aerial tissues. Acts as a negative regulator of cold acclimation. Involved in water economy as well as salt tolerance. Regulated at the transcriptional level by NAC012/SND1 (Probable). KEYWORDS: 3D-structure;Acetylation;Disulfide bond;Glycoprotein;Golgi apparatus;Membrane;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus membrane ; Single-pass type II membrane protein MISCELLANEOUS: Contains 2 motifs that are conserved in esterases, but it is unlikely that this protein belongs to the catalytically active pectin esterases." Q9LY75,"PROTEIN NAMES: Peptidyl-prolyl cis-trans isomerase CYP63 (AtCYP63) (PPIase CYP63) (Cyclophilin-63) (Cyclophilin-like protein CypRS64) PROTEIN FAMILY: Cyclophilin-type PPIase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the cyclophilin-type PPIase family. FUNCTION: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. May be implicated in the folding, transport, and assembly of proteins. Probably involved in early steps of spliceosomal assembly. KEYWORDS: Alternative splicing;Isomerase;Nucleus;Phosphoprotein;Reference proteome;Rotamase SUBCELLULAR LOCATION: Nucleus, nucleoplasm Nucleus speckle Note=Moves from nuclear bodies to nuclear speckles upon interaction with SR proteins. " Q9LYA9,"PROTEIN NAMES: Chloroplast stem-loop binding protein of 41 kDa a, chloroplastic (CSP41-a) PROTEIN FAMILY: NAD(P)-dependent epimerase/dehydratase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the NAD(P)-dependent epimerase/dehydratase family. FUNCTION: Binds and cleaves RNA, particularly in stem-loops. Associates with pre-ribosomal particles in chloroplasts, and participates in chloroplast ribosomal RNA metabolism, probably during the final steps of 23S rRNA maturation. May enhance transcription by the plastid-encoded polymerase and translation in plastid via the stabilization of ribosome assembly intermediates. Required for chloroplast integrity. Involved in the regulation of the circadian system. KEYWORDS: Chloroplast;Phosphoprotein;Plastid;Reference proteome;RNA-binding;rRNA processing;rRNA-binding;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast, plastoglobule Note=Present in stromules. " Q9LYC1,"PROTEIN NAMES: Gibberellin receptor GID1B (AtCXE14) (Carboxylesterase 14) (GID1-like protein 2) (Protein GA INSENSITIVE DWARF 1B) (AtGID1B) PROTEIN FAMILY: 'GDXG' lipolytic enzyme family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the 'GDXG' lipolytic enzyme family. FUNCTION: Functions as a soluble gibberellin (GA) receptor. GA is an essential hormone that regulates growth and development in plants. Binds with high affinity the biologically active gibberellin GA4, but has no affinity for the biologically inactive GAs. In response to GA, interacts with specific DELLA proteins, known as repressors of GA-induced growth, and targets them for degradation via proteasome. Seems to be required for GA signaling that controls root growth, seed germination and flower development. May function as a dominant GA receptor at low GA concentrations in germination. Partially redundant with GID1A and GID1C. KEYWORDS: Acetylation;Gibberellin signaling pathway;Hydrolase;Nucleus;Receptor;Reference proteome SUBCELLULAR LOCATION: Nucleus " Q9LYU3,"PROTEIN NAMES: Ethylene-responsive transcription factor ERF113 (Protein RELATED TO AP2 6L) PROTEIN FAMILY: AP2/ERF transcription factor family, ERF subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the AP2/ERF transcription factor family. ERF subfamily. FUNCTION: Transcriptional activator involved in the regulation of plant development and tolerance to abiotic stresses. Acts as positive regulator of tolerance to waterlogging stress. Delays waterlogging-induced premature senescence by regulating stomatal closure and antioxidant enzyme activity. May function through ABI1-mediated abscisic acid (ABA) signaling pathway. Involved in tissue reunion of wounded inflorescence stems. Required for the division of pith cells in the reunion process, which is dependent on polar-transported auxin and the wound-inducible hormones ethylene and jasmonate. Binds to the GCC-box pathogenesis-related promoter element. May be involved in the regulation of gene expression by stress factors and by components of stress signal transduction pathways (By similarity). KEYWORDS: Activator;DNA-binding;Ethylene signaling pathway;Nucleus;Reference proteome;Stress response;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " Q9LYU7,"PROTEIN NAMES: Inactive leucine-rich repeat receptor-like protein kinase CORYNE (Protein SUPPRESSOR OF OVEREXPRESSION OF LLP1 2) PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. FUNCTION: Involved in the perception of CLV3 and CLV3-like (CLE) peptides, that act as extracellular signals regulating meristem maintenance. Modulates root, shoot and flower apical meristem maintenance and floral organ development regulation, probably via CLAVATA (CLV)-like pathways involving at least CLV3 and CLE19. In complex with CLV2, perceives secreted CLV3-like effector proteins from plant-parasitic cyst nematodes as ligand mimics of the plant CLE signaling pathway. This recognition is required for proper feeding structure (syncytium) development and ultimately successful nematode infection. CLE14 perception by CLV2/CRN complex triggers root meristem differentiation. Required for the sensing of the root CLE peptides (e.g. CLE8, CLE9/CLE10, CLE11, CLE13, CLE14, CLE16, CLE17, CLE18, CLE20, CLE21, CLE25, CLE26, CLE40, CLE41/CLE44 and CLE45), which also involves CLV2 and leads to root growth regulation, mostly in the phloem and protophloem. Promotes the accumulation of BAM3, especially at later stages of protophloem development. KEYWORDS: Alternative splicing;ATP-binding;Cell membrane;Endoplasmic reticulum;Membrane;Nucleotide-binding;Reference proteome;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein Endoplasmic reticulum membrane ; Single-pass type I membrane protein Note=Requires CLV2 for export from the endoplasmic reticulum and localization to the plasma membrane. " Q9LZ82,"PROTEIN NAMES: Protein BTR1 (Binding to ToMV RNA 1) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Negative regulator of tomato mosaic virus (ToMV) multiplication, but has no effect on the multiplication of cucumber mosaic virus (CMV). Limits the spreading of the virus. Isoform BTR1S: binds preferentially and directly to the 5'terminal region of ToMV genomic RNA, and affects the efficiency of translation rather than mRNA stability. KEYWORDS: Acetylation;Alternative splicing;Cytoplasm;Phosphoprotein;Reference proteome;Repeat;RNA-binding SUBCELLULAR LOCATION: Cytoplasm " Q9LZD0,"PROTEIN NAMES: Purine-uracil permease NCS1 (Nucleobase cation symporter 1) (AtNCS1) (Plastidic nucleobase transporter) (Uracil/purine transport protein NCS1) PROTEIN FAMILY: Purine-cytosine permease (2.A.39) family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the purine-cytosine permease (2.A.39) family. FUNCTION: Nucleobase-proton symporter that facilitates the uptake of nucleobases in the cells. Can transport adenine, guanine and uracil. Contributes to uracil import into plastids for plastidic uracil salvage which is essential for plant growth and development. KEYWORDS: Chloroplast;Membrane;Plastid;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Plastid, chloroplast envelope Plastid, chloroplast membrane ; Multi-pass membrane protein " Q9LZD3,"PROTEIN NAMES: Exocyst complex component EXO70A1 (AtExo70a1) (Exocyst subunit Exo70 family protein A1) PROTEIN FAMILY: EXO70 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the EXO70 family. FUNCTION: Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane during regulated or polarized secretion. Involved in polarized cell growth and organ morphogenesis. Involved in polarized cell growth and organ morphogenesis. During cytokinesis, involved in cell plate initiation, cell plate maturation and formation of new primary cell wall. Participates in polarized pectin delivery required for the polarized development of the mucilage-producing volcano cells of the seed coat. Involved in the recycling and localization of auxin efflux carriers PIN1 and PIN2, and thus in polar auxin transport regulation. Functions in vesicle trafficking in tracheary elements to regulate patterned secondary cell wall (SCW) thickening. KEYWORDS: 3D-structure;Alternative splicing;Cell membrane;Cell wall;Cytoplasm;Cytoskeleton;Exocytosis;Membrane;Reference proteome;Secreted;Transport SUBCELLULAR LOCATION: Cytoplasm, cytosol Cytoplasm, cytoskeleton, phragmoplast Cell membrane Secreted, cell wall Note=During cytokinesis, localizes to the nascent cell plate and later to the cell plate insertion site and along the post-cytokinetic wall. Localized at vesicle-like small compartments at cortical microtubules, especially in the presence of FPP3/VETH1, FPP2/VETH2 and COG2. Confined to helical/annular plasma membrane (PM) domains in protoxylem of roots before the secondary cell wall (SCW) is deposited. After the induction of xylem differentiation, first associated with plasma membrane (PM) foci and later co-localizes with microtubules (MT) organized into regular bundles, especially at the cell cortex. " Q9LZI2,"PROTEIN NAMES: UDP-glucuronic acid decarboxylase 2 (UDP-XYL synthase 2) (UDP-glucuronate decarboxylase 2) (UGD) (UXS-2) (dTDP-glucose 4-6-dehydratase homolog D18) PROTEIN FAMILY: NAD(P)-dependent epimerase/dehydratase family, UDP-glucuronic acid decarboxylase subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the NAD(P)-dependent epimerase/dehydratase family. UDP-glucuronic acid decarboxylase subfamily. FUNCTION: Catalyzes the NAD-dependent decarboxylation of UDP-glucuronic acid to UDP-xylose. Necessary for the biosynthesis of the core tetrasaccharide in glycosaminoglycan biosynthesis. PATHWAY: Nucleotide-sugar biosynthesis; UDP-alpha-D-xylose biosynthesis; UDP-alpha-D-xylose from UDP-alpha-D-glucuronate: step 1/1. KEYWORDS: Acetylation;Decarboxylase;Golgi apparatus;Lyase;Membrane;NAD;Reference proteome;Signal-anchor;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus, Golgi stack membrane ; Single-pass type II membrane protein " Q9LZL3,PROTEIN NAMES: Aspartic proteinase PCS1 (Aspartic protease 38) (AtASP38) (Protein EMBRYO DEFECTIVE 24) (Protein PROMOTION OF CELL SURVIVAL 1) PROTEIN FAMILY: Peptidase A1 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the peptidase A1 family. FUNCTION: Embryo-specific aspartic protease that limits programmed cell death during reproductive development. Possesses peptidase activity toward casein in vitro. KEYWORDS: Aspartyl protease;Endoplasmic reticulum;Glycoprotein;Hydrolase;Protease;Reference proteome;Signal;Zymogen SUBCELLULAR LOCATION: Endoplasmic reticulum. MISCELLANEOUS: Ectopic expression of PCS1 induces survival of cells of the anther wall including stomium and septum cells. This leads to a failure in anther dehiscence and subsequent male sterility. Q9LZM8,"PROTEIN NAMES: BEL1-like homeodomain protein 9 (BEL1-like protein 9) (Protein BELLRINGER) (Protein LARSON) (Protein PENNYWISE) (Protein REPLUMLESS) (Protein VAAMANA) PROTEIN FAMILY: TALE/BELL homeobox family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the TALE/BELL homeobox family. FUNCTION: Transcription factor that is involved in the preservation of the spiral phyllotactic arrangement leading to a regular pattern of organ initiation. Required for maintenance of stem cell fate in the shoot apical meristem, and is essential for specifying floral primordia and establishing early internode patterning events during inflorescence development. Acts as a transcription repressor of AG expression in floral and inflorescence meristems. Is also responsive of the nuclear import of SHOOT MERISTEMLESS (STM). In the fruit, plays a central role in patterning by negatively regulating SHP expression in order to prevent replum cells from adopting a valve margin cell fate. KEYWORDS: DNA-binding;Homeobox;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " Q9LZS0,"PROTEIN NAMES: Trihelix transcription factor PTL (Trihelix DNA-binding protein PETAL LOSS) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Transcription factor that prevents growth. Regulates perianth architecture in flower, mostly in the second whorl, probably by suppressing growth between initiating sepals, ensuring that they remain separate, and by modulating organ shapes. Required for the establishment of auxin flux. KEYWORDS: Developmental protein;DNA-binding;Growth regulation;Nucleus;Reference proteome;Repeat;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus. " Q9M084,"PROTEIN NAMES: Aspartate--tRNA ligase 2, cytoplasmic (Aspartyl-tRNA synthetase) (AspRS) (Protein IMPAIRED IN BABA-INDUCED DISEASE IMMUNITY 1) PROTEIN FAMILY: Class-II aminoacyl-tRNA synthetase family, Type 2 subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family. Type 2 subfamily. FUNCTION: Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA (By similarity). Involved in the perception of beta-aminobutyric acid (BABA) and required for BABA priming effect in disease resistance. KEYWORDS: Acetylation;Aminoacyl-tRNA synthetase;ATP-binding;Cytoplasm;DNA-binding;Endoplasmic reticulum;Ligase;Magnesium;Metal-binding;Nucleotide-binding;Protein biosynthesis;Reference proteome SUBCELLULAR LOCATION: Cytoplasm, cytosol Endoplasmic reticulum " Q9M0G0,PROTEIN NAMES: Glutathione hydrolase 3 (Gamma-glutamyltransferase 3) (Gamma-glutamyltranspeptidase 3) (Gamma-glutamyltranspeptidase 4) PROTEIN FAMILY: Gamma-glutamyltransferase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the gamma-glutamyltransferase family. FUNCTION: May play a role in protecting plants from some xenobiotic chemicals by degrading vacuolar glutathione conjugates into cysteine conjugates. PATHWAY: Sulfur metabolism; glutathione metabolism. KEYWORDS: Acyltransferase;Alternative splicing;Detoxification;Glycoprotein;Hydrolase;Membrane;Reference proteome;Transferase;Transmembrane;Transmembrane helix;Vacuole SUBCELLULAR LOCATION: Vacuole membrane ; Single-pass membrane protein Q9M0K4,"PROTEIN NAMES: Transcription factor LAF1 (Myb-related protein 18) (AtMYB18) (Protein LONG AFTER FAR-RED LIGHT 1) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Transcription factor that promotes photomorphogenesis in the light by participating in the transmission of phytochrome A (phyA) signals to downstream responses. Probably acts by activating expression of light-induced genes. In darkness, its degradation prevents the activation of light-induced genes. KEYWORDS: Activator;DNA-binding;Nucleus;Phytochrome signaling pathway;Reference proteome;Repeat;Transcription;Transcription regulation;Ubl conjugation SUBCELLULAR LOCATION: Nucleus speckle " Q9M1S3,PROTEIN NAMES: Probable RNA-binding protein ARP1 (ABA-regulated RNA-binding protein 1) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Probable RNA-binding protein involved in the regulation of abscisic acid (ABA) response during seed germination. May regulate transcript levels of several germination-responsive genes under ABA. KEYWORDS: Alternative splicing;Nucleus;Reference proteome;RNA-binding;Stress response SUBCELLULAR LOCATION: Nucleus Q9M2G7,PROTEIN NAMES: Lipid phosphate phosphatase delta (AtLPPD) (Phosphatidic acid phosphatase delta) (Sphingoid phosphate phosphatase 1) (AtSSP1) (Sphingosine-1-phosphate phosphatase) (AtSPPASE) PROTEIN FAMILY: Type 2 lipid phosphate phosphatase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the type 2 lipid phosphate phosphatase family. FUNCTION: Functions as a sphingoid long-chain base phosphate (LCBP) phosphatase. May play a role in the regulation of LCBP levels and be involved in stomatal responses through LCBP-mediated ABA signaling. KEYWORDS: Alternative splicing;Endoplasmic reticulum;Hydrolase;Lipid metabolism;Membrane;Reference proteome;Sphingolipid metabolism;Stress response;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein Q9M2Z3,"PROTEIN NAMES: Single-strand DNA endonuclease 1 (AtSEND1) (Flap endonuclease GEN-like 2) (XPG-like endonuclease 2) PROTEIN FAMILY: XPG/RAD2 endonuclease family, GEN subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the XPG/RAD2 endonuclease family. GEN subfamily. FUNCTION: Endonuclease which cleaves flap structures at the junction between single-stranded DNA and double-stranded DNA with a specific cleavage site in the 5' overhang strand exactly one nucleotide 3' of the branch point. Structure- and sequence-specific nuclease that resolves holliday junctions (HJs) by symmetrically oriented incisions in two opposing strands near the junction point, thus leading to ligatable products; HJs are physical links between homologous DNA molecules that arise as central intermediary structures during homologous recombination and repair in meiotic and somatic cells. Structure-specific nuclease with 5'-flap endonuclease activity, preferentially cleaving static flaps 5' overhang strand exactly one nucleotide in the 3' direction of the branch point and, to lower extent, on the two neighboring positions. Also able to cleave double-stranded flap strand 1 one nucleotide in the 3' direction of the branch point. Together with MUS81, essential for the resolution of toxic replication structures to ensure genome stability, and to maintain telomere integrity and replication. KEYWORDS: Alternative splicing;DNA damage;DNA repair;Endonuclease;Hydrolase;Magnesium;Metal-binding;Nuclease;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: [Isoform 2]: May be due to an intron retention." Q9M336,"PROTEIN NAMES: Uracil phosphoribosyltransferase, chloroplastic (UPRTase) (UMP pyrophosphorylase) PROTEIN FAMILY: UPRTase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the UPRTase family. FUNCTION: Uracil phosphoribosyltransferase (UPRT) that catalyzes the conversion of uracil and 5-phospho-alpha-D-ribose 1-diphosphate (PRPP) to UMP and diphosphate. Is probably the only functional UPRT, since the dual-domain proteins of the UKL family seem to lack this activity. PATHWAY: Pyrimidine metabolism; UMP biosynthesis via salvage pathway; UMP from uracil: step 1/1. KEYWORDS: Acetylation;Allosteric enzyme;Alternative splicing;Chloroplast;Glycosyltransferase;GTP-binding;Nucleotide-binding;Plastid;Reference proteome;Transferase;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast " Q9M3B0,PROTEIN NAMES: Probable pectinesterase/pectinesterase inhibitor 34 [Includes: Pectinesterase inhibitor 34 (Pectin methylesterase inhibitor 34); Pectinesterase 34 (PE 34) (Pectin methylesterase 34) (AtPME34)] PROTEIN FAMILY: PMEI family; Pectinesterase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: In the N-terminal section; belongs to the PMEI family.; SIMILARITY: In the C-terminal section; belongs to the pectinesterase family. FUNCTION: Acts in the modification of cell walls via demethylesterification of cell wall pectin. PATHWAY: Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5. KEYWORDS: Alternative splicing;Aspartyl esterase;Cell wall biogenesis/degradation;Disulfide bond;Hydrolase;Membrane;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Single-pass membrane protein MISCELLANEOUS: The PMEI region may act as an autoinhibitory domain and prevent untimely PME activity during transport. Q9M3H5,"PROTEIN NAMES: Probable cadmium/zinc-transporting ATPase HMA1, chloroplastic (Protein HEAVY METAL ATPASE 1) PROTEIN FAMILY: Cation transport ATPase (P-type) family, Type IB subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily. FUNCTION: Involved in cadmium/zinc transport. KEYWORDS: ATP-binding;Cadmium;Chloroplast;Magnesium;Membrane;Metal-binding;Nucleotide-binding;Plastid;Plastid inner membrane;Reference proteome;Transit peptide;Translocase;Transmembrane;Transmembrane helix;Zinc SUBCELLULAR LOCATION: Plastid, chloroplast inner membrane ; Multi-pass membrane protein " Q9M651,"PROTEIN NAMES: RAN GTPase-activating protein 2 (AtRanGAP2) (RanGAP2) PROTEIN FAMILY: RNA1 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the RNA1 family. FUNCTION: GTPase activator for the nuclear Ras-related regulatory protein Ran, converting it to the putatively inactive GDP-bound state. KEYWORDS: Cytoplasm;Cytoskeleton;GTPase activation;Leucine-rich repeat;Membrane;Nucleus;Reference proteome;Repeat SUBCELLULAR LOCATION: Cytoplasm. Nucleus membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, phragmoplast. Note=Localized in patchy areas at the nuclear envelope of interphase cells. During mitosis, associates with mitotic spindles at the anaphase. Associated to the microtubular phragmoplast and the surface of the daughter nuclei at the telophase. " Q9M7Q3,"PROTEIN NAMES: ABSCISIC ACID-INSENSITIVE 5-like protein 6 (Abscisic acid responsive elements-binding factor 3) (ABRE-binding factor 3) (Dc3 promoter-binding factor 5) (AtDPBF5) (bZIP transcription factor 37) (AtbZIP37) PROTEIN FAMILY: BZIP family, ABI5 subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the bZIP family. ABI5 subfamily. FUNCTION: Binds to the ABA-responsive element (ABRE). Mediates stress-responsive ABA signaling. KEYWORDS: Abscisic acid signaling pathway;Activator;Alternative splicing;DNA-binding;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: [Isoform 2]: May be due to intron retention." Q9M8R4,"PROTEIN NAMES: Protein DJ-1 homolog D (AtDJ1D) (Lactoylglutathione lyase DJ1D) PROTEIN FAMILY: Peptidase C56 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the peptidase C56 family. FUNCTION: Possesses glyoxalase I activity. Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione. May be involved in oxidative stress response. KEYWORDS: 3D-structure;Lyase;Oxidation;Reference proteome;Repeat;Stress response " Q9M8Y0,"PROTEIN NAMES: Probable UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase SEC (Protein SECRET AGENT) PROTEIN FAMILY: Glycosyltransferase 41 family, O-GlcNAc transferase subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the glycosyltransferase 41 family. O-GlcNAc transferase subfamily. FUNCTION: O-linked N-acetylglucosamine transferase (OGT) that mediates O-glycosylation of capsid protein (CP) of virus in case of infection by Plum pox virus. OGTs catalyze the addition of nucleotide-activated sugars directly onto the polypeptide through O-glycosidic linkage with the hydroxyl of serine or threonine. Probably acts by adding O-linked sugars to yet unknown proteins. Its OGT activity has been proved in vitro but not in vivo. Required with SPY for gamete and seed development. Mediates O-glycosylation of the DELLA protein RGA, a repressor of the gibberellin (GA) signaling pathway. O-glycosylation by SEC inhibits RGA binding to four of its interactors PIF3, PIF4, JAZ1, and BZR1 that are key regulators in light, jasmonate, and brassinosteroid signaling pathways, respectively. Activates ATX1 through O-GlcNAc modification to augment ATX1-mediated H3K4me3 histone epigenetic modification at FLC locus, thus preventing premature flowering. PATHWAY: Protein modification; protein glycosylation. KEYWORDS: Developmental protein;Glycosyltransferase;Reference proteome;Repeat;TPR repeat;Transferase " Q9M8Z5,"PROTEIN NAMES: Guanine nucleotide-binding protein-like NSN1 (Nucleolar GTP-binding protein NSN1) (DAR GTPase 4) (Protein nucleostemin-like 1) PROTEIN FAMILY: TRAFAC class YlqF/YawG GTPase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the TRAFAC class YlqF/YawG GTPase family. FUNCTION: Involved in the differentiation of epidermal cells, probably via the regulation of the expression of meristem-related genes (e.g. CLV3, STM, KNAT1, CUC2 and AG) and of leaf polarity-related genes (e.g. YAB5, FIL, AS2, PHB and PHV). May play a role in regulating cellular proliferation (By similarity). Necessary for flower development, probably by preventing apical dominance through the down-regulation of AG expression. Required for embryogenesis, leaf and cotyledon development, as well as for leaf polarity establishment. Plays an important role in plant growth and senescence by modulating ribosome biogenesis in nucleolus. Possesses GTPAse activity in vitro. Possesses RNA binding activity in vitro. Associates with ribosomes. KEYWORDS: Coiled coil;Developmental protein;GTP-binding;Nucleotide-binding;Nucleus;Reference proteome;Repeat SUBCELLULAR LOCATION: Nucleus Nucleus, nucleolus Note=Shuttles between the nucleus and nucleolus. " Q9M8Z7,"PROTEIN NAMES: Sterol 3-beta-glucosyltransferase UGT80A2 (UDP-glucose:sterol glucosyltransferase 80A2) PROTEIN FAMILY: Glycosyltransferase 28 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the glycosyltransferase 28 family. FUNCTION: Involved in the biosynthesis of sterol glucosides. Catalyzes the synthesis of steryl glycosides (SGs) and acyl steryl glycosides (ASGs) which are the most abundant sterol derivatives in higher plants. Can act on several sterols like sitosterol, campesterol and stigmasterol. Both UGT80A2 and UGT80B1 are required for the normal production of SGs and ASGs in seeds. KEYWORDS: Alternative splicing;Glycosyltransferase;Lipid biosynthesis;Lipid metabolism;Reference proteome;Steroid biosynthesis;Steroid metabolism;Sterol biosynthesis;Sterol metabolism;Transferase " Q9M903,"PROTEIN NAMES: Protein TRIGALACTOSYLDIACYLGLYCEROL 4, chloroplastic (Protein PIGMENT DEFECTIVE 320) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Involved in lipid transfer from the endoplasmic reticulum (ER) to plastids. Specifically binds phosphatidic acid (PtdOH). KEYWORDS: Alternative splicing;Chloroplast;Endoplasmic reticulum;Lipid transport;Membrane;Plastid;Plastid outer membrane;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Plastid, chloroplast outer membrane ; Single-pass membrane protein Endoplasmic reticulum Note=According to previous research localizes to the ER (GFP experiment), but previous research found that TDG4 was located in the chloroplast outer envelope using cell fractionation. " Q9M9C5,"PROTEIN NAMES: Probable leucine-rich repeat receptor-like protein kinase At1g68400 PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. KEYWORDS: ATP-binding;Cell membrane;Glycoprotein;Kinase;Leucine-rich repeat;Membrane;Nucleotide-binding;Phosphoprotein;Receptor;Reference proteome;Repeat;Serine/threonine-protein kinase;Signal;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein " Q9M9E0,"PROTEIN NAMES: L-type lectin-domain containing receptor kinase S.1 (LecRK-S.1) PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family; Leguminous lectin family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: In the C-terminal section; belongs to the protein kinase superfamily. Ser/Thr protein kinase family.; SIMILARITY: In the N-terminal section; belongs to the leguminous lectin family. FUNCTION: Involved in resistance response to the pathogenic oomycetes Phytophthora infestans and Phytophthora capsici and to the pathogenic bacteria Pseudomonas syringae. KEYWORDS: ATP-binding;Cell membrane;Glycoprotein;Kinase;Lectin;Membrane;Nucleotide-binding;Plant defense;Receptor;Reference proteome;Serine/threonine-protein kinase;Signal;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein " Q9M9F5,"PROTEIN NAMES: 9-cis-epoxycarotenoid dioxygenase NCED9, chloroplastic (AtNCED9) PROTEIN FAMILY: Carotenoid oxygenase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the carotenoid oxygenase family. FUNCTION: Has a 11,12(11',12') 9-cis epoxycarotenoid cleavage activity. Catalyzes the first step of abscisic-acid biosynthesis from carotenoids. Contributes probably to abscisic acid synthesis for the induction of seed dormancy. KEYWORDS: Abscisic acid biosynthesis;Chloroplast;Dioxygenase;Iron;Metal-binding;Oxidoreductase;Plastid;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast stroma " Q9M9G6,"PROTEIN NAMES: Cleavage stimulating factor 64 (AtCstF-64) (AtCstF64) (CF-1 64 kDa subunit) (Cleavage stimulation factor 64 kDa subunit) (CSTF 64 kDa subunit) (Protein ENHANCED SILENCING PHENOTYPE 1) (Protein SUPPRESSORS OF OVEREXPRESSED FCA 19) (SOF19) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: One of the multiple factors required for polyadenylation and 3'-end cleavage of pre-mRNAs (By similarity). This subunit is directly involved in the binding to pre-mRNAs, especially on the 3' non-coding region. Required for the targeted 3' processing of antisense transcripts that triggers transcriptional silencing of the corresponding sense gene. KEYWORDS: mRNA processing;Nucleus;Reference proteome;RNA-binding;RNA-mediated gene silencing SUBCELLULAR LOCATION: Nucleus " Q9M9H7,PROTEIN NAMES: Carboxypeptidase SOL1 (Protein SUPPRESSOR OF LLP1 1) PROTEIN FAMILY: Peptidase M14 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the peptidase M14 family. FUNCTION: Possesses in vitro carboxypeptidase activity against the C-terminal arginine and lysine residues. Involved in the maturation of CLE19. Removes the C-terminal arginine residue of CLE19 proprotein. The cleavage of the C-terminal arginine residue is necessary for CLE19 activity in vivo. Is not involved in generating active CLV3. Is not involved in CLE19 or CLV3 perception. KEYWORDS: Alternative splicing;Carboxypeptidase;Endosome;Glycoprotein;Hydrolase;Membrane;Metal-binding;Protease;Reference proteome;Signal;Transmembrane;Transmembrane helix;Zinc SUBCELLULAR LOCATION: Endosome membrane ; Single-pass type I membrane protein Q9M9S0,PROTEIN NAMES: Zinc-finger homeodomain protein 4 (AtZHD4) (Homeobox protein 31) (AtHB-31) (Protein FLORAL TRANSITION AT THE MERISTEM 2) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Putative transcription factor. Probably involved in the regulation of floral induction. KEYWORDS: DNA-binding;Homeobox;Metal-binding;Nucleus;Reference proteome;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Q9MA15,"PROTEIN NAMES: Protein ACTIVITY OF BC1 COMPLEX KINASE 3, chloroplastic (ABC1-LIKE KINASE 3) (Protein REPRESSOR OF BDR1) PROTEIN FAMILY: Protein kinase superfamily, ADCK protein kinase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the protein kinase superfamily. ADCK protein kinase family. FUNCTION: Kinase that can phosphorylate the tocopherol cyclase VTE1, a key enzyme of tocopherol (vitamin E) metabolism and involved in the recycling of oxidated alpha-tocopherol quinone, possibly stabilizing it at plastoglobules. Regulates also membrane prenylquinone composition. Required for photooxidative stress responses to prevent photosystem II core and chlorophyll degradations. Together with ABC1K1, contributes to plastoglobule (PG) function in prenyl-lipid metabolism, stress response, and thylakoid remodeling. Promotes photodamage of chloroplasts under continuous red light, thus working in opposition to ABC1K1. KEYWORDS: Alternative splicing;ATP-binding;Chloroplast;Kinase;Nucleotide-binding;Plastid;Reference proteome;Stress response;Transferase;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast, plastoglobule Plastid, chloroplast Note=Colocalizes with the chlorophyll autofluorescence of mature chloroplasts. In cotyledons of seedlings grown in continuous red light, only observed in the chloroplasts losing chlorophyll autofluorescence. " Q9MA17,"PROTEIN NAMES: Protein SOMBRERO (NAC domain-containing protein 33) (ANAC033) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Transcription regulator. Together with BRN1 and BRN2, regulates cellular maturation of root cap. Represses stem cell-like divisions in the root cap daughter cells, and thus promotes daughter cell fate. Inhibits expression of its positive regulator FEZ in a feedback loop for controlled switches in cell division plane. Promotes the expression of genes involved in secondary cell walls (SCW) biosynthesis. KEYWORDS: Developmental protein;DNA-binding;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " Q9MAT5,"PROTEIN NAMES: Protein arginine N-methyltransferase PRMT10 PROTEIN FAMILY: Class I-like SAM-binding methyltransferase superfamily, Protein arginine N-methyltransferase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N-methyltransferase family. FUNCTION: Methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in some proteins. Essential for regulating flowering time. KEYWORDS: 3D-structure;Alternative splicing;Methyltransferase;Reference proteome;S-adenosyl-L-methionine;Transferase MISCELLANEOUS: [Isoform 2]: May be due to a competing acceptor splice site." Q9MAU3,PROTEIN NAMES: Transcription initiation factor TFIID subunit 6 (Protein EMBRYO DEFECTIVE 2781) (TATA box associated factor II 59) (TBP-associated factor 6) (AtTAF6) (Transcription initiation factor TFIID subunit D5) PROTEIN FAMILY: TAF6 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the TAF6 family. FUNCTION: TAFs are components of the transcription factor IID (TFIID) complex that is essential for mediating regulation of RNA polymerase transcription. Required for proper pollen function. May stabilize the interaction of TFIID with selected promoters. Not redundant with TAF6B. KEYWORDS: Activator;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus Q9MBA2,"PROTEIN NAMES: Septum site-determining protein minD homolog, chloroplastic (AtMinD1) (Protein ACCUMULATION AND REPLICATION OF CHLOROPLASTS 11) (Septum site-determining protein MinD1) PROTEIN FAMILY: ParA family, MinD subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the ParA family. MinD subfamily. FUNCTION: Together with ARC3 and MCD1, regulates FtsZ ring positioning in chloroplasts in an ARC6-dependent manner. Calcium-dependent ATPase required for the correct placement of the plastid division site. Inhibits FtsZ filament and ring formation in the plastid. Mediates inhibition of plastid division. In cooperation with MINE1, prevents FtsZ ring formation anywhere outside of the mid-plastids. KEYWORDS: ATP-binding;Chloroplast;Membrane;Nucleotide-binding;Plastid;Plastid inner membrane;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast inner membrane ; Peripheral membrane protein Note=Attached to membranes in punctate structures dispersed on the inner envelope and at the mid-chloroplast division site in an ATPase activity-dependent manner. Also present at the chloroplast to a ring structure at the constriction site. " Q9MCI8,"PROTEIN NAMES: Immunoglobulin-binding protein EibD (Trimeric autotransporter adhesin EibD) (TAA EibD) (Type 5 secretion system autotransporter EibD) PROTEIN FAMILY: Autotransporter-2 (AT-2) family, Eib subfamily ORGANISM: Escherichia coli SIMILARITY: Belongs to the autotransporter-2 (AT-2) (TC 1.B.40) family. Eib subfamily. FUNCTION: Binds (in a non-immune fashion) to the Fc portion of human IgA and IgG; binding occurs on the cell surface. Confers the ability to survive exposure to human serum exposure. Binds to the Fc portion of human IgG, IgA and to whole mouse antibodies also via Fc. Upon overexpression cells acquire an extra cell surface layer that forms a zipper-like contact between cells; cells autoagglutinate and form biofilm more readily, suggesting it may play a role in defense against a host. KEYWORDS: 3D-structure;Cell outer membrane;Coiled coil;Membrane;Protein transport;Signal;Transmembrane;Transmembrane beta strand;Transport SUBCELLULAR LOCATION: Cell surface Cell outer membrane ; Multi-pass membrane protein Note=The C-terminal translocator domain is localized in the outer membrane and the passenger domain is at the cell surface. MISCELLANEOUS: Encoded in a prophage region of strain ECOR 9, upon UV treatment bacteriophage containing this gene can be isolated." Q9N0N3,"PROTEIN NAMES: Transcription factor AP-2-alpha (AP2-alpha) (AP-2 transcription factor) (Activating enhancer-binding protein 2-alpha) (Activator protein 2) (AP-2) PROTEIN FAMILY: AP-2 family ORGANISM: Ovis aries (Sheep) SIMILARITY: Belongs to the AP-2 family. FUNCTION: Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-alpha is the only AP-2 protein required for early morphogenesis of the lens vesicle. Together with the CITED2 coactivator, stimulates the PITX2 P1 promoter transcription activation. Associates with chromatin to the PITX2 P1 promoter region (By similarity). KEYWORDS: Activator;Alternative splicing;DNA-binding;Isopeptide bond;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation;Ubl conjugation SUBCELLULAR LOCATION: Nucleus " Q9N363,"PROTEIN NAMES: mRNA-decapping enzyme 1 PROTEIN FAMILY: DCP1 family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the DCP1 family. FUNCTION: Component of the decapping complex necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay (By similarity). In contrast to orthologs, does not possess decapping activity and does not remove the 7-methyl guanine cap structure from mRNA molecules. In the nervous system, negatively regulates the expression of insulin-like peptide ins-7, which in turn promotes longevity. This may in part be through promoting the activity of daf-16 in distal tissues. Required for the developmental axon guidance and regrowth of PLM touch receptor neurons. In ADL sensory neurons, plays a role in ciliary shape formation. Acts in neurons to promote larval survival at high temperatures by negatively regulating lin-14 expression (Probable). KEYWORDS: Cytoplasm;mRNA processing;Nonsense-mediated mRNA decay;Reference proteome;RNA-binding SUBCELLULAR LOCATION: Cytoplasm Cytoplasmic granule Note=Diffusely localized in the cytoplasm, but accumulates in puncta during pronuclear migration in embryos. After pronuclear migration, accumulates in P-granules which segregated to the posterior cell, and smaller puncta scattered in the cytoplasm. Localizes to cytoplasmic puncta in neuronal cell bodies of neurons such as touch receptor neurons. In some cytoplasmic puncta in touch receptor neurons, co-localizes with car-1. " Q9N373,"PROTEIN NAMES: E3 ubiquitin-protein ligase makorin (Leptoderan male tail protein 2) (RING-type E3 ubiquitin transferase makorin) ORGANISM: Caenorhabditis elegans FUNCTION: E3 ubiquitin ligase which catalyzes the covalent attachment of ubiquitin moieties onto substrate proteins (By similarity). Promotes the larval to adult transition by binding to the long non-coding RNA lep-5 to target the heterochronic protein lin-28 for degradation by the proteasome. This association and degradation of lin-28 also controls the timing of the sexual differentiation of individual neurons in males including the AIM, AWA, ADF, ASJ and CEM neurons. Plays a role in governing the developmental timing of male tail tip morphogenesis. Plays a role in two aspects of male mating behavior: response to hermaphrodite contact and vulva location. May play a role in the detection of preferred food sources. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Alternative splicing;Cytoplasm;Differentiation;Metal-binding;Reference proteome;Repeat;RNA-binding;Sexual differentiation;Transferase;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cytoplasm " Q9N3Q8,"PROTEIN NAMES: Dauer abnormal formation protein 25 (daf-25) (suppressor of che-2 small body size 3) (Chb-3) ORGANISM: Caenorhabditis elegans FUNCTION: May be involved in the trafficking and dendritic transport of signaling proteins, such as the receptor-type guanylate cyclases gcy-12 and daf-11, to the cilia. In ciliated sensory neurons, required for the calcium flux to the cytoplasm in response to onset and removal of a nitric oxide (NO) stimulus and is thereby required for the behavioral avoidance response to NO-producing organisms like P.aeruginosa. KEYWORDS: ANK repeat;Cell projection;Cilium;Metal-binding;Reference proteome;Repeat;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cell projection, cilium " Q9N3Z3,"PROTEIN NAMES: Serine/threonine-protein kinase chk-1 PROTEIN FAMILY: Protein kinase superfamily, CAMK Ser/Thr protein kinase family, NIM1 subfamily ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. NIM1 subfamily. FUNCTION: Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest and activation of DNA repair in response to the presence of DNA damage or unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. Required for checkpoint mediated cell cycle arrest in response to DNA damage in germline cells. Delays cell-cycle reentry of the Z2 and Z3 primordial germ cells in response to transcription-induced DNA damage as they emerge from cell cycle arrest in L1 larvae. Essential for embryogenesis. KEYWORDS: Alternative splicing;ATP-binding;Cell cycle;Cytoplasm;Developmental protein;DNA damage;Kinase;Nucleotide-binding;Nucleus;Phosphoprotein;Reference proteome;Serine/threonine-protein kinase;Transferase SUBCELLULAR LOCATION: Cytoplasm Nucleus Cytoplasm, perinuclear region Note=The Ser-344 phosphorylated form colocalizes with P granules in a perinuclear manner in embryonic germline precursor cells and in Z2/Z3 primordial germ cells in L1 stage larvae. " Q9N491,"PROTEIN NAMES: Beta-1,3-galactosyltransferase sqv-2 (Squashed vulva protein 2) PROTEIN FAMILY: Glycosyltransferase 31 family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the glycosyltransferase 31 family. FUNCTION: Beta-1,3-galactosyltransferase that transfers galactose from UDP-galactose to substrates with a terminal beta-linked galactose residue. Required for vulval morphogenesis and zygotic cytokinesis, suggesting that glycosaminoglycans play a central role in vulval morphogenesis. PATHWAY: Glycan metabolism; chondroitin sulfate biosynthesis.; PATHWAY: Glycan metabolism; heparan sulfate biosynthesis. KEYWORDS: Glycoprotein;Glycosyltransferase;Golgi apparatus;Manganese;Membrane;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus membrane ; Single-pass type II membrane protein " Q9N4A7,"PROTEIN NAMES: Protein SEC13 homolog (CeSEH13R) (GATOR complex protein SEC13) (Nuclear pore complex protein 20) PROTEIN FAMILY: WD repeat SEC13 family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the WD repeat SEC13 family. FUNCTION: Functions as a component of the nuclear pore complex (NPC) and the COPII coat (By similarity). Required for the nuclear import of hcp-4 during mitotic prophase, this step is essential for centrosome assembly and resolution.; FUNCTION: As a component of the GATOR complex may function in the amino acid-sensing branch of the TORC1 signaling pathway. KEYWORDS: Alternative splicing;Cell cycle;Cell division;Cytoplasmic vesicle;Endoplasmic reticulum;ER-Golgi transport;Lysosome;Membrane;Mitosis;mRNA transport;Nuclear pore complex;Nucleus;Protein transport;Reference proteome;Repeat;Translocation;Transport;WD repeat SUBCELLULAR LOCATION: Cytoplasmic vesicle, COPII-coated vesicle membrane ; Peripheral membrane protein ; Cytoplasmic side Endoplasmic reticulum membrane ; Peripheral membrane protein ; Cytoplasmic side Nucleus, nuclear pore complex Lysosome membrane Note=Localizes close to endoplasmic reticulum exit sites (ERES) and the nuclear envelopes of proliferating germ nuclei. " Q9N4X8,"PROTEIN NAMES: Glutathione S-transferase P 10 (GST 5.4) (GST class-pi) (GSTP2-2) PROTEIN FAMILY: GST superfamily, Pi family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the GST superfamily. Pi family. FUNCTION: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Responsible for approximately one-third of 4-hydroxy-2-nonenal conjugation. May play a role in the detoxification of reactive oxygen species produced during pathogenic bacterial infection. KEYWORDS: Direct protein sequencing;Reference proteome;Transferase " Q9N589,"PROTEIN NAMES: Myotubularin-related protein 1 (Phosphatidylinositol-3,5-bisphosphate 3-phosphatase) (Phosphatidylinositol-3-phosphate phosphatase) PROTEIN FAMILY: Protein-tyrosine phosphatase family, Non-receptor class myotubularin subfamily ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the protein-tyrosine phosphatase family. Non-receptor class myotubularin subfamily. FUNCTION: Dephosphorylates phosphatidylinositol 3-phosphate (PI3P) and phosphatidylinositol 3,5-bisphosphate (PI(3,5)P2). Negatively regulates accumulation of PI3P on intracellular vesicles. Negatively regulates phagocytosis of apoptotic cells probably by limiting the recruitment and/or the activation of ced-5, ced-2 and ced-12 complex. In addition, may positively regulate phagosome maturation by promoting recycling of apoptotic receptor ced-1 back to the plasma membrane. Essential for embryonic and larval development. May promote migration of distal tip cells. KEYWORDS: Cell membrane;Cell projection;Coiled coil;Hydrolase;Lipid metabolism;Membrane;Phagocytosis;Reference proteome SUBCELLULAR LOCATION: Cell membrane ; Peripheral membrane protein Cell projection, phagocytic cup Apical cell membrane ; Peripheral membrane protein Cytoplasmic granule membrane Note=Transiently co-localizes with phagocytic receptor ced-1 at the pseudopods during phagocytosis of apoptotic cells. " Q9NAS8,"PROTEIN NAMES: CLIP domain-containing serine protease B14 [Cleaved into: CLIP domain-containing serine protease B14 light chain; CLIP domain-containing serine protease B14 heavy chain] PROTEIN FAMILY: Peptidase S1 family, CLIP subfamily ORGANISM: Anopheles gambiae (African malaria mosquito) SIMILARITY: Belongs to the peptidase S1 family. CLIP subfamily. FUNCTION: Serine protease (By similarity). Plays a role in innate immunity against infections by parasite P.berghei and by Gram-negative bacteria such as E.coli. In response to P.berghei infection, contributes to the clearing of parasite ookinetes independent of melanization, an innate immune response which consists in the deposition of melanin pigments on invading pathogens and parasites. May play a role in non-septic wound healing. KEYWORDS: Disulfide bond;Glycoprotein;Hydrolase;Immunity;Innate immunity;Protease;Reference proteome;Secreted;Serine protease;Signal SUBCELLULAR LOCATION: Secreted Note=Secreted in the hemolymph. " Q9NDE8,"PROTEIN NAMES: MIP-related peptides (AMRPs) [Cleaved into: GAAPKFF-amide; GQAPRFF-amide; AMAPKFF-amide; GSPRFF-amide; AAPRFF-amide; QAPRFF-amide; GSPHFI-amide; SDPFFM-amide; GAPRFL-amide; GAPRFI-amide; GAPRFV-amide; GPPRFI-amide; QAPRFI-amide; LWVPGMV-amide] ORGANISM: Aplysia californica (California sea hare) FUNCTION: Has some structural and functional features similar to vertebrate opioid peptides. AMRPs are inhibitory on Aplysia esophagus, penis retractor muscle, and body wall muscle. KEYWORDS: Amidation;Cleavage on pair of basic residues;Neuropeptide;Pyrrolidone carboxylic acid;Secreted;Signal SUBCELLULAR LOCATION: Secreted " Q9NDH7,"PROTEIN NAMES: Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase (GlcNAc-TV) (Glycosylation-related protein 2) (N-acetylglucosaminyltransferase gly-2) PROTEIN FAMILY: Glycosyltransferase 18 family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the glycosyltransferase 18 family. FUNCTION: Catalyzes the addition of N-acetylglucosamine (GlcNAc) in beta 1-6 linkage to the alpha-linked mannose of biantennary N-linked oligosaccharides. PATHWAY: Protein modification; protein glycosylation. KEYWORDS: Glycoprotein;Glycosyltransferase;Golgi apparatus;Membrane;Metal-binding;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus membrane ; Single-pass type II membrane protein " Q9NDM2,"PROTEIN NAMES: Protein trapped in endoderm-1 PROTEIN FAMILY: G-protein coupled receptor 1 family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the G-protein coupled receptor 1 family. FUNCTION: Essential for the first active step of germ cell migration: transepithelial migration of germ cells through the posterior midgut (PMG) epithelium. KEYWORDS: Cell membrane;G-protein coupled receptor;Glycoprotein;Membrane;Phosphoprotein;Receptor;Reference proteome;Transducer;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein MISCELLANEOUS: Overexpression of the Tre1 gene restores the taste sensitivity to trehalose in a Tre1 mutant. This experiment cannot be explained given that other authors demonstrate that trehalose sensitivity maps to the adjacent gene, Gr5a." Q9NEQ0,"PROTEIN NAMES: Omega-3 fatty acid desaturase fat-1 (FAT-1) (FAT-1 N3 desaturase) (Fatty acid desaturase 1) (Fatty acid metabolism 1) PROTEIN FAMILY: Fatty acid desaturase type 1 family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the fatty acid desaturase type 1 family. FUNCTION: Omega-3 fatty acid desaturase that recognizes a range of 18- and 20-carbon omega-6 substrates. Introduces a double bond in the fatty acid chain three carbons away from terminal methyl group to biosynthesize n-3 (omega-3) polyunsaturated fatty acids (PUFAs) endogenously (PUFAs are essential for membrane structure and many cellular and physiological processes). Acts on a number of substrates like linoleoyl-CoA ((9Z,12Z)-octadecadienoyl-CoA, 18:2n-6), dihomo-gamma-linolenoyl-CoA ((8Z,11Z,14Z)-eicosatrienoyl-CoA, 20:3n-6), and arachidonoyl-CoA ((5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA, 20:4n-6), to generate alpha-linolenoyl-CoA ((9Z,12Z,15Z)-octadecatrienoyl-CoA, 18:3n-3), (8Z,11Z,14Z,17Z)-eicosatetraenoyl-CoA (20:4n-3) and (5Z,8Z,11Z,14Z,17Z)-eicosapentaenoyl-CoA (20:5n-3) respectively. Unlike plants, Caenorhabditis elegans desaturases seem to use fatty acyl-CoAs as substrates (By similarity). PATHWAY: Lipid metabolism; polyunsaturated fatty acid biosynthesis. KEYWORDS: Fatty acid biosynthesis;Fatty acid metabolism;Lipid biosynthesis;Lipid metabolism;Membrane;Oxidoreductase;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Multi-pass membrane protein MISCELLANEOUS: HPO-19 and T05H4.4 are cytochrome b5 reductases required for PUFA desaturation in Caenorhabditis elegans. HPO-19 knockdown or mutation alters FAT-1 desaturase activity. Although FAT-1 lacks a cytochrome b5 domain, its N-terminal contains a DUF3474 domain that may possess some oxidoreductase activity for electron transfer, obviating the need for an extra cytochrome b5 enzyme, but still requiring cytochrome b5 reductase HPO-19/T05H4.4 to become activated." Q9NEZ7,"PROTEIN NAMES: Bardet-Biedl syndrome 1 protein homolog ORGANISM: Caenorhabditis elegans FUNCTION: Component of the BBSome complex (By similarity). The BBSome complex is thought to function as a coat complex required for sorting of specific membrane proteins to the primary cilia (By similarity). The BBSome complex is required for ciliogenesis but is dispensable for centriolar satellite function (By similarity). Required for proper BBSome complex assembly and its ciliary localization. Required for cilia biogenesis and both the assembly and movement of intraflagellar transport proteins along the ciliary axoneme. KEYWORDS: Cell projection;Cilium;Cilium biogenesis/degradation;Cytoplasm;Cytoskeleton;Protein transport;Reference proteome;Transport SUBCELLULAR LOCATION: Cell projection, cilium Cytoplasm, cytoskeleton, cilium basal body Cytoplasm, cytoskeleton, cilium axoneme " Q9NFT7,PROTEIN NAMES: Hexokinase type 2 PROTEIN FAMILY: Hexokinase family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the hexokinase family. FUNCTION: Catalyzes the phosphorylation of various hexoses to hexose 6-phosphate. PATHWAY: Carbohydrate metabolism; hexose metabolism.; PATHWAY: Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 1/4. KEYWORDS: ATP-binding;Glycolysis;Kinase;Nucleotide-binding;Reference proteome;Transferase Q9NFU0,"PROTEIN NAMES: Fragile X messenger ribonucleoprotein 1 homolog (FMRP) (dFMR1) PROTEIN FAMILY: FMR1 family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the FMR1 family. FUNCTION: Polyribosome-associated RNA-binding protein that plays a role in neuronal development and synaptic plasticity through the regulation of protein synthesis of mRNAs. Acts as an mRNA regulator by mediating formation of some phase-separated membraneless compartment: undergoes liquid-liquid phase separation upon binding to target mRNAs, leading to assemble mRNAs into cytoplasmic ribonucleoprotein granules that concentrate mRNAs with associated regulatory factors. Mainly acts as an activator of mRNA translation: promotes translation of stored mRNAs in oocytes. Can also act as a negative translational regulator of specific mRNAs. Represses translation of the microtubule-associated protein futsch mRNA to regulate microtubule-dependent synaptic growth and function. Localizes to specific N6-methyladenosine (m6A)-containing RNAs as part of a complex with the m6A reader Ythdf and thereby regulates axonal growth in the mushroom bodies and neuromuscular junctions. Specifically recognizes and binds a subset of N6-methyladenosine (m6A)-containing mRNAs in embryos, promoting formation of a phase-separated membraneless compartment that mediates degradation of maternal mRNAs. May also be involved in microRNA (miRNA)-mediated translational suppression as part of the RNA-induced silencing complex (RISC). Required for stability of the central pair of microtubules in the spermatid axoneme. Regulates photoreceptor structure and neuromuscular junction (NMJ) neurotransmission in the eye. During embryogenesis, involved in germline fate determination. KEYWORDS: Alternative splicing;Cell projection;Cytoplasm;Neurogenesis;Phosphoprotein;Reference proteome;Repeat;Repressor;Ribonucleoprotein;RNA-binding;RNA-mediated gene silencing;Sensory transduction;Synapse;Translation regulation;Vision SUBCELLULAR LOCATION: Cytoplasm, Cytoplasmic ribonucleoprotein granule Cytoplasm Perikaryon Cell projection, neuron projection Synapse Cytoplasm, Stress granule Note=Localizes in the neuronal soma and cell processes, and in pre- and postsynaptic neurons, as well as in postsynaptic muscles. " Q9NHV9,PROTEIN NAMES: Protein vav (DroVav) (dVAV) ORGANISM: Drosophila melanogaster (Fruit fly) FUNCTION: Couples tyrosine kinase signals with the activation of the Rho/Rac GTPases. Probably plays a pivotal role as a signal transducer protein during fruit fly development. KEYWORDS: Alternative splicing;Guanine-nucleotide releasing factor;Metal-binding;Phosphoprotein;Reference proteome;Repeat;SH2 domain;SH3 domain;Zinc;Zinc-finger Q9NKV0,PROTEIN NAMES: Myeloid leukemia factor (Myelodysplasia-myeloid leukemia factor) (dMLF) PROTEIN FAMILY: MLF family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the MLF family. KEYWORDS: Alternative splicing;Cytoplasm;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Q9NKX1,"PROTEIN NAMES: Endoplasmin homolog (92 kDa phosphoprotein) (Glucose-regulated protein 94 homolog) (GRP-94 homolog) PROTEIN FAMILY: Heat shock protein 90 family ORGANISM: Dictyostelium discoideum (Social amoeba) SIMILARITY: Belongs to the heat shock protein 90 family. FUNCTION: May play a role in late differentiation as well as in starvation response. When overexpressed, suppresses the ability to form normal fruiting bodies and impairs prespore differentiation as well as maturation into spores. KEYWORDS: ATP-binding;Calcium;Chaperone;Endoplasmic reticulum;Glycoprotein;Golgi apparatus;Nucleotide-binding;Phosphoprotein;Reference proteome;Signal SUBCELLULAR LOCATION: Endoplasmic reticulum. Golgi apparatus. Note=In prespore cells, preferentially localizes in Golgi vesicles and cisternae. Colocalizes with trap1 in the prespore-specific vacuole. " Q9NLA3,"PROTEIN NAMES: Nucleoplasmin-like protein ANO39 (39 kDa oocyte-expressed nucleolar protein) (Nucleic acid-associated protein 36) (Nucleic acid-binding nuclear protein) (NAAP) (NAAP1) (NAAP2) PROTEIN FAMILY: Nucleoplasmin family ORGANISM: Patiria pectinifera (Starfish) (Asterina pectinifera) SIMILARITY: Belongs to the nucleoplasmin family. FUNCTION: Binds double-stranded RNA and both single-stranded and double-stranded DNA. KEYWORDS: Acetylation;Cytoplasm;Direct protein sequencing;DNA-binding;Glycoprotein;Nucleus;Phosphoprotein;RNA-binding SUBCELLULAR LOCATION: Nucleus, nucleolus Cytoplasm Note=In oocytes, expression is limited to the germinal vesicle with highest levels in the nucleolus although a considerable portion is present in other regions of the germinal vesicle. In oocytes that progress to the first meiotic metaphase, diffuse expression is observed throughout the egg cytoplasm. " Q9NP31,PROTEIN NAMES: SH2 domain-containing protein 2A (SH2 domain-containing adapter protein) (T cell-specific adapter protein) (TSAd) (VEGF receptor-associated protein) ORGANISM: Homo sapiens (Human) FUNCTION: Could be a T-cell-specific adapter protein involved in the control of T-cell activation. May play a role in the CD4-p56-LCK-dependent signal transduction pathway. Could also play an important role in normal and pathological angiogenesis. Could be an adapter protein that facilitates and regulates interaction of KDR with effector proteins important to endothelial cell survival and proliferation. KEYWORDS: Alternative splicing;Angiogenesis;Cytoplasm;Developmental protein;Differentiation;Phosphoprotein;Reference proteome;SH2 domain;SH3-binding SUBCELLULAR LOCATION: Cytoplasm. Q9NP59,"PROTEIN NAMES: Solute carrier family 40 member 1 (Ferroportin-1) (Iron-regulated transporter 1) PROTEIN FAMILY: Ferroportin (FP) family, SLC40A subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the ferroportin (FP) (TC 2.A.100) family. SLC40A subfamily. FUNCTION: Transports Fe(2+) from the inside of a cell to the outside of the cell, playing a key role for maintaining systemic iron homeostasis. Transports iron from intestinal, splenic, hepatic cells, macrophages and erythrocytes into the blood to provide iron to other tissues (By similarity). Controls therefore dietary iron uptake, iron recycling by macrophages and erythrocytes, and release of iron stores in hepatocytes (By similarity). When iron is in excess in serum, circulating HAMP/hepcidin levels increase resulting in a degradation of SLC40A1, thus limiting the iron efflux to plasma. KEYWORDS: 3D-structure;Cell membrane;Disease variant;Glycoprotein;Ion transport;Iron;Iron transport;Membrane;Metal-binding;Reference proteome;Transmembrane;Transmembrane helix;Transport;Ubl conjugation SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Basolateral cell membrane ; Multi-pass membrane protein Note=Localized to the basolateral membrane of hepatocytoma WIF-B cells. " Q9NP94,"PROTEIN NAMES: Zinc transporter ZIP2 (6A1) (Eti-1) (Solute carrier family 39 member 2) (Zrt- and Irt-like protein 2) (ZIP-2) (hZIP2) PROTEIN FAMILY: ZIP transporter family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the ZIP transporter (TC 2.A.5) family. FUNCTION: Transporter for the divalent cation Zn(2+). Mediates the influx of Zn(2+) into cells from extracellular space. The Zn(2+) uniporter activity is independent of H(+)-driving force, but is modulated by extracellular pH and membrane potential. Transports also other divalent cations Zn(2+), Cd2(+), Cu2(+), Co2(+) in the order of decreasing affinity, respectively. In the skin, aids in the differentiation of keratinocytes in the epidermis (By similarity). KEYWORDS: Alternative splicing;Cell membrane;Ion transport;Membrane;Reference proteome;Transmembrane;Transmembrane helix;Transport;Zinc;Zinc transport SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein " Q9NPA1,"PROTEIN NAMES: Calcium-activated potassium channel subunit beta-3 (BK channel subunit beta-3) (BKbeta3) (Hbeta3) (Calcium-activated potassium channel, subfamily M subunit beta-3) (Charybdotoxin receptor subunit beta-3) (K(VCA)beta-3) (Maxi K channel subunit beta-3) (Slo-beta-3) PROTEIN FAMILY: KCNMB family, KCNMB3 subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the KCNMB (TC 8.A.14.1) family. KCNMB3 subfamily. FUNCTION: Regulatory subunit of the calcium activated potassium KCNMA1 (maxiK) channel. Modulates the calcium sensitivity and gating kinetics of KCNMA1, thereby contributing to KCNMA1 channel diversity. Alters the functional properties of the current expressed by the KCNMA1 channel. Isoform 2, isoform 3 and isoform 4 partially inactivate the current of KCNBMA. Isoform 4 induces a fast and incomplete inactivation of KCNMA1 channel that is detectable only at large depolarizations. In contrast, isoform 1 does not induce detectable inactivation of KCNMA1. Two or more subunits of KCNMB3 are required to block the KCNMA1 tetramer. KEYWORDS: Alternative splicing;Disulfide bond;Glycoprotein;Ion channel;Ion transport;Membrane;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. " Q9NQ25,"PROTEIN NAMES: SLAM family member 7 (CD2 subset 1) (CD2-like receptor-activating cytotoxic cells) (CRACC) (Membrane protein FOAP-12) (Novel Ly9) (Protein 19A) (CD antigen CD319) ORGANISM: Homo sapiens (Human) FUNCTION: Self-ligand receptor of the signaling lymphocytic activation molecule (SLAM) family. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. Activities are controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2. Isoform 1 mediates NK cell activation through a SH2D1A-independent extracellular signal-regulated ERK-mediated pathway. Positively regulates NK cell functions by a mechanism dependent on phosphorylated SH2D1B. Downstream signaling implicates PLCG1, PLCG2 and PI3K. In addition to heterotypic NK cells-target cells interactions also homotypic interactions between NK cells may contribute to activation. However, in the absence of SH2D1B, inhibits NK cell function. Acts also inhibitory in T-cells (By similarity). May play a role in lymphocyte adhesion. In LPS-activated monocytes negatively regulates production of pro-inflammatory cytokines.; FUNCTION: Isoform 3 does not mediate any NK cell activation. KEYWORDS: Adaptive immunity;Alternative splicing;Direct protein sequencing;Disulfide bond;Glycoprotein;Immunity;Immunoglobulin domain;Innate immunity;Membrane;Receptor;Reference proteome;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. MISCELLANEOUS: Proposed to be involved in systemic lupus erythematosus (SLE) disease process." Q9NQ79,"PROTEIN NAMES: Cartilage acidic protein 1 (68 kDa chondrocyte-expressed protein) (CEP-68) (ASPIC) ORGANISM: Homo sapiens (Human) KEYWORDS: Alternative splicing;Disulfide bond;EGF-like domain;Extracellular matrix;Glycoprotein;Reference proteome;Repeat;Secreted;Signal SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix MISCELLANEOUS: [Isoform 1]: Shares an exon with the neighboring tail-to-tail oriented gene GOLGA7B." Q9NQG7,"PROTEIN NAMES: BLOC-3 complex member HPS4 (Hermansky-Pudlak syndrome 4 protein) (Light-ear protein homolog) ORGANISM: Homo sapiens (Human) FUNCTION: Component of the BLOC-3 complex, a complex that acts as a guanine exchange factor (GEF) for RAB32 and RAB38, promotes the exchange of GDP to GTP, converting them from an inactive GDP-bound form into an active GTP-bound form. The BLOC-3 complex plays an important role in the control of melanin production and melanosome biogenesis and promotes the membrane localization of RAB32 and RAB38. KEYWORDS: Albinism;Alternative splicing;Guanine-nucleotide releasing factor;Hermansky-Pudlak syndrome;Phosphoprotein;Reference proteome " Q9NQQ7,"PROTEIN NAMES: Solute carrier family 35 member C2 (Ovarian cancer-overexpressed gene 1 protein) PROTEIN FAMILY: TPT transporter family, SLC35C subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the TPT transporter family. SLC35C subfamily. FUNCTION: May play an important role in the cellular response to tissue hypoxia. May be either a GDP-fucose transporter that competes with SLC35C1 for GDP-fucose, or a factor that otherwise enhances the fucosylation of Notch and is required for optimal Notch signaling in mammalian cells. KEYWORDS: Alternative splicing;Glycoprotein;Golgi apparatus;Membrane;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Golgi apparatus, cis-Golgi network membrane ; Multi-pass membrane protein Endoplasmic reticulum-Golgi intermediate compartment membrane ; Multi-pass membrane protein " Q9NQR1,"PROTEIN NAMES: N-lysine methyltransferase KMT5A (H4-K20-HMTase KMT5A) (Histone-lysine N-methyltransferase KMT5A) (Lysine N-methyltransferase 5A) (Lysine-specific methylase 5A) (PR/SET domain-containing protein 07) (PR-Set7) (PR/SET07) (SET domain-containing protein 8) PROTEIN FAMILY: Class V-like SAM-binding methyltransferase superfamily, Histone-lysine methyltransferase family, PR/SET subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. PR/SET subfamily. FUNCTION: Protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins. Specifically monomethylates 'Lys-20' of histone H4 (H4K20me1). H4K20me1 is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. Required for cell proliferation, probably by contributing to the maintenance of proper higher-order structure of DNA during mitosis. Involved in chromosome condensation and proper cytokinesis. Nucleosomes are preferred as substrate compared to free histones. Mediates monomethylation of p53/TP53 at 'Lys-382', leading to repress p53/TP53-target genes. Plays a negative role in TGF-beta response regulation and a positive role in cell migration. KEYWORDS: 3D-structure;Acetylation;Alternative splicing;Cell cycle;Cell division;Chromatin regulator;Chromosome;Coiled coil;Direct protein sequencing;Methyltransferase;Mitosis;Nucleus;Phosphoprotein;Reference proteome;Repressor;S-adenosyl-L-methionine;Transcription;Transcription regulation;Transferase;Ubl conjugation SUBCELLULAR LOCATION: Nucleus Chromosome Note=Specifically localizes to mitotic chromosomes. Colocalized with SIRT2 at mitotic foci. Associates with chromosomes during mitosis; association is increased in a H(2)O(2)-induced oxidative stress-dependent manner. Associates with silent chromatin on euchromatic arms. Not associated with constitutive heterochromatin. " Q9NR21,"PROTEIN NAMES: Protein mono-ADP-ribosyltransferase PARP11 (ADP-ribosyltransferase diphtheria toxin-like 11) (ARTD11) (Poly [ADP-ribose] polymerase 11) (PARP-11) PROTEIN FAMILY: ARTD/PARP family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the ARTD/PARP family. FUNCTION: Mono-ADP-ribosyltransferase that mediates mono-ADP-ribosylation of target proteins. Plays a role in nuclear envelope stability and nuclear remodeling during spermiogenesis (By similarity). KEYWORDS: 3D-structure;ADP-ribosylation;Alternative splicing;Differentiation;Glycosyltransferase;mRNA transport;NAD;Nuclear pore complex;Nucleotidyltransferase;Nucleus;Protein transport;Reference proteome;Spermatogenesis;Transferase;Translocation;Transport SUBCELLULAR LOCATION: Nucleus, nuclear pore complex Note=Colocalizes with NUP153 at nuclear pores. MISCELLANEOUS: [Isoform 4]: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay." Q9NRH2,"PROTEIN NAMES: SNF-related serine/threonine-protein kinase (SNF1-related kinase) PROTEIN FAMILY: Protein kinase superfamily, CAMK Ser/Thr protein kinase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. FUNCTION: May play a role in hematopoietic cell proliferation or differentiation. Potential mediator of neuronal apoptosis. KEYWORDS: 3D-structure;Alternative splicing;ATP-binding;Kinase;Magnesium;Metal-binding;Methylation;Nucleotide-binding;Nucleus;Phosphoprotein;Reference proteome;Serine/threonine-protein kinase;Transferase SUBCELLULAR LOCATION: Nucleus " Q9NRI5,"PROTEIN NAMES: Disrupted in schizophrenia 1 protein ORGANISM: Homo sapiens (Human) FUNCTION: Involved in the regulation of multiple aspects of embryonic and adult neurogenesis. Required for neural progenitor proliferation in the ventrical/subventrical zone during embryonic brain development and in the adult dentate gyrus of the hippocampus (By similarity). Participates in the Wnt-mediated neural progenitor proliferation as a positive regulator by modulating GSK3B activity and CTNNB1 abundance. Plays a role as a modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including neuron positioning, dendritic development and synapse formation (By similarity). Inhibits the activation of AKT-mTOR signaling upon interaction with CCDC88A (By similarity). Regulates the migration of early-born granule cell precursors toward the dentate gyrus during the hippocampal development. Inhibits ATF4 transcription factor activity in neurons by disrupting ATF4 dimerization and DNA-binding (By similarity). Plays a role, together with PCNT, in the microtubule network formation. KEYWORDS: 3D-structure;Alternative splicing;Chromosomal rearrangement;Coiled coil;Cytoplasm;Cytoskeleton;Developmental protein;Isopeptide bond;Microtubule;Mitochondrion;Neurogenesis;Reference proteome;Schizophrenia;Synapse;Ubl conjugation;Wnt signaling pathway SUBCELLULAR LOCATION: Cytoplasm Cytoplasm, cytoskeleton Mitochondrion Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Postsynaptic density Note=Colocalizes with NDEL1 in the perinuclear region and the centrosome (By similarity). Localizes to punctate cytoplasmic foci which overlap in part with mitochondria. Colocalizes with PCNT at the centrosome. MISCELLANEOUS: [Isoform 1]: Non-canonical donor and acceptor splice sites for the last 2 exons.; MISCELLANEOUS: [Isoform 2]: Non-canonical donor and acceptor splice sites for the last 2 exons." Q9NRM6,"PROTEIN NAMES: Interleukin-17 receptor B (IL-17 receptor B) (IL-17RB) (Cytokine receptor-like 4) (IL-17 receptor homolog 1) (IL-17Rh1) (IL17Rh1) (Interleukin-17B receptor) (IL-17B receptor) ORGANISM: Homo sapiens (Human) FUNCTION: Receptor for the pro-inflammatory cytokines IL17B and IL17E. May play a role in controlling the growth and/or differentiation of hematopoietic cells. KEYWORDS: 3D-structure;Alternative splicing;Cell membrane;Direct protein sequencing;Glycoprotein;Membrane;Receptor;Reference proteome;Secreted;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: [Isoform 1]: Cell membrane; Single-pass type I membrane protein.; SUBCELLULAR LOCATION: [Isoform 2]: Secreted. MISCELLANEOUS: [Isoform 2]: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay." Q9NRQ2,"PROTEIN NAMES: Phospholipid scramblase 4 (PL scramblase 4) (Ca(2+)-dependent phospholipid scramblase 4) (Cell growth-inhibiting gene 43 protein) (TRA1) PROTEIN FAMILY: Phospholipid scramblase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the phospholipid scramblase family. FUNCTION: May mediate accelerated ATP-independent bidirectional transbilayer migration of phospholipids upon binding calcium ions that results in a loss of phospholipid asymmetry in the plasma membrane. May play a central role in the initiation of fibrin clot formation, in the activation of mast cells and in the recognition of apoptotic and injured cells by the reticuloendothelial system. KEYWORDS: 3D-structure;Alternative splicing;Calcium;Lipoprotein;Membrane;Palmitate;Phosphoprotein;Reference proteome;Repeat;SH3-binding;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Single-pass type II membrane protein " Q9NRR2,"PROTEIN NAMES: Tryptase gamma (Serine protease 31) (Transmembrane tryptase) [Cleaved into: Tryptase gamma light chain; Tryptase gamma heavy chain] PROTEIN FAMILY: Peptidase S1 family, Tryptase subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the peptidase S1 family. Tryptase subfamily. KEYWORDS: Disulfide bond;Glycoprotein;Hydrolase;Membrane;Protease;Reference proteome;Serine protease;Signal;Transmembrane;Transmembrane helix;Zymogen SUBCELLULAR LOCATION: Membrane ; Single-pass membrane protein " Q9NRS4,"PROTEIN NAMES: Transmembrane protease serine 4 (Channel-activating protease 2) (CAPH2) (Membrane-type serine protease 2) (MT-SP2) [Cleaved into: Transmembrane protease serine 4 catalytic chain] PROTEIN FAMILY: Peptidase S1 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the peptidase S1 family. FUNCTION: Plasma membrane-anchored serine protease that directly induces processing of pro-uPA/PLAU into the active form through proteolytic activity. Seems to be capable of activating ENaC (By similarity).; FUNCTION: (Microbial infection) In gut epithelial cells, facilitates human coronavirus SARS-CoV-2 infection through, at least, the cleavage of coronavirus spike glycoproteins which activates the glycoprotein for host cell entry. KEYWORDS: Alternative splicing;Cell membrane;Disulfide bond;Glycoprotein;Host-virus interaction;Hydrolase;Membrane;Protease;Reference proteome;Secreted;Serine protease;Signal-anchor;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type II membrane protein.; SUBCELLULAR LOCATION: [Transmembrane protease serine 4 catalytic chain]: Secreted Note=Activated by cleavage and secreted. " Q9NRZ9,"PROTEIN NAMES: Lymphoid-specific helicase (Proliferation-associated SNF2-like protein) (SWI/SNF2-related matrix-associated actin-dependent regulator of chromatin subfamily A member 6) PROTEIN FAMILY: SNF2/RAD54 helicase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the SNF2/RAD54 helicase family. FUNCTION: Plays an essential role in normal development and survival. Involved in regulation of the expansion or survival of lymphoid cells. Required for de novo or maintenance DNA methylation. May control silencing of the imprinted CDKN1C gene through DNA methylation. May play a role in formation and organization of heterochromatin, implying a functional role in the regulation of transcription and mitosis (By similarity). KEYWORDS: Alternative splicing;ATP-binding;Cell cycle;Cell division;Coiled coil;Developmental protein;Disease variant;Helicase;Hydrolase;Mitosis;Nucleotide-binding;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus Note=Closely associated with pericentric heterochromatin. " Q9NS68,PROTEIN NAMES: Tumor necrosis factor receptor superfamily member 19 (TRADE) (Toxicity and JNK inducer) ORGANISM: Homo sapiens (Human) FUNCTION: Can mediate activation of JNK and NF-kappa-B. May promote caspase-independent cell death. KEYWORDS: Alternative splicing;Apoptosis;Direct protein sequencing;Disulfide bond;Glycoprotein;Membrane;Receptor;Reference proteome;Repeat;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Single-pass type I membrane protein Q9NS84,"PROTEIN NAMES: Carbohydrate sulfotransferase 7 (Chondroitin 6-sulfotransferase 2) (C6ST-2) (Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 5) (GST-5) (N-acetylglucosamine 6-O-sulfotransferase 4) (GlcNAc6ST-4) (Gn6st-4) PROTEIN FAMILY: Sulfotransferase 1 family, Gal/GlcNAc/GalNAc subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the sulfotransferase 1 family. Gal/GlcNAc/GalNAc subfamily. FUNCTION: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues. Preferentially acts on mannose-linked GlcNAc. Also able to catalyze the transfer of sulfate to position 6 of the N-acetylgalactosamine (GalNAc) residue of chondroitin. Also acts on core 2 mucin-type oligosaccharide and N-acetyllactosamine oligomer with a lower efficiency. Has weak or no activity toward keratan sulfate and oligosaccharides containing the Galbeta1-4GlcNAc. Catalyzes 6-O-sulfation of beta-benzyl GlcNAc but not alpha- or beta-benzyl GalNAc. KEYWORDS: Carbohydrate metabolism;Glycoprotein;Golgi apparatus;Membrane;Phosphoprotein;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus membrane ; Single-pass type II membrane protein " Q9NSC7,"PROTEIN NAMES: Alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase 1 (GalNAc alpha-2,6-sialyltransferase I) (ST6GalNAc I) (ST6GalNAc-I) (ST6GalNAcI) (hST6GalNAc-I) (Sialyltransferase 7A) (SIAT7-A) PROTEIN FAMILY: Glycosyltransferase 29 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the glycosyltransferase 29 family. FUNCTION: Protein sialyltransferase specifically expressed in goblet cells that plays a key role in intestinal host-commensal homeostasis. Conjugates sialic acid with an alpha-2-6 linkage to N-acetylgalactosamine (GalNAc) glycan chains linked to serine or threonine in glycoproteins. Catalyzes the formation of the sialyl-Tn (S-Tn) antigen, an antigen found in intestinal goblet cells, as well as ulcerative colitis (UC) and various cancers. Protein sialylation in globlet cells is essential for mucus integrity and is required to protect the intestinal mucus against excessive bacterial proteolytic degradation. PATHWAY: Protein modification; protein glycosylation. KEYWORDS: Disease variant;Disulfide bond;Glycoprotein;Glycosyltransferase;Golgi apparatus;Membrane;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus membrane ; Single-pass type II membrane protein " Q9NSE2,"PROTEIN NAMES: Cytokine-inducible SH2-containing protein (CIS) (CIS-1) (Protein G18) (Suppressor of cytokine signaling) (SOCS) ORGANISM: Homo sapiens (Human) FUNCTION: SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. CIS is involved in the negative regulation of cytokines that signal through the JAK-STAT5 pathway such as erythropoietin, prolactin and interleukin 3 (IL3) receptor. Inhibits STAT5 trans-activation by suppressing its tyrosine phosphorylation. May be a substrate-recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (By similarity). PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Alternative splicing;Growth regulation;Reference proteome;SH2 domain;Signal transduction inhibitor;Ubl conjugation;Ubl conjugation pathway " Q9NST1,"PROTEIN NAMES: 1-acylglycerol-3-phosphate O-acyltransferase PNPLA3 (Acylglycerol transacylase) (Adiponutrin) (ADPN) (Calcium-independent phospholipase A2-epsilon) (iPLA2-epsilon) (Lysophosphatidic acid acyltransferase) (Patatin-like phospholipase domain-containing protein 3) ORGANISM: Homo sapiens (Human) FUNCTION: Specifically catalyzes coenzyme A (CoA)-dependent acylation of 1-acyl-sn-glycerol 3-phosphate (2-lysophosphatidic acid/LPA) to generate phosphatidic acid (PA), an important metabolic intermediate and precursor for both triglycerides and glycerophospholipids. Does not esterify other lysophospholipids. Acyl donors are long chain (at least C16) fatty acyl-CoAs: arachidonoyl-CoA, linoleoyl-CoA, oleoyl-CoA and at a lesser extent palmitoyl-CoA. Additionally possesses low triacylglycerol lipase and CoA-independent acylglycerol transacylase activities and thus may play a role in acyl-chain remodeling of triglycerides. In vitro may express hydrolytic activity against glycerolipids triacylglycerol, diacylglycerol and monoacylglycerol, with a strong preference for oleic acid as the acyl moiety. However, the triacylglycerol hydrolase activity is controversial and may be very low. Possesses phospholipase A2 activity. PATHWAY: Phospholipid metabolism.; PATHWAY: Glycerolipid metabolism. KEYWORDS: Acyltransferase;Alternative splicing;Disease variant;Glycoprotein;Hydrolase;Lipid biosynthesis;Lipid droplet;Lipid metabolism;Membrane;Obesity;Phospholipid biosynthesis;Phospholipid metabolism;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Single-pass type II membrane protein Lipid droplet " Q9NTG7,"PROTEIN NAMES: NAD-dependent protein deacetylase sirtuin-3, mitochondrial (hSIRT3) (Regulatory protein SIR2 homolog 3) (SIR2-like protein 3) PROTEIN FAMILY: Sirtuin family, Class I subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the sirtuin family. Class I subfamily. FUNCTION: NAD-dependent protein deacetylase. Activates or deactivates mitochondrial target proteins by deacetylating key lysine residues. Known targets include ACSS1, IDH, GDH, SOD2, PDHA1, LCAD, SDHA and the ATP synthase subunit ATP5PO. Contributes to the regulation of the cellular energy metabolism. Important for regulating tissue-specific ATP levels. In response to metabolic stress, deacetylates transcription factor FOXO3 and recruits FOXO3 and mitochondrial RNA polymerase POLRMT to mtDNA to promote mtDNA transcription. Acts as a regulator of ceramide metabolism by mediating deacetylation of ceramide synthases CERS1, CERS2 and CERS6, thereby increasing their activity and promoting mitochondrial ceramide accumulation (By similarity). Regulates hepatic lipogenesis. Uses NAD(+) substrate imported by SLC25A47, triggering downstream activation of PRKAA1/AMPK-alpha signaling cascade that ultimately downregulates sterol regulatory element-binding protein (SREBP) transcriptional activities and ATP-consuming lipogenesis to restore cellular energy balance. KEYWORDS: 3D-structure;Alternative splicing;Metal-binding;Mitochondrion;NAD;Reference proteome;Transferase;Transit peptide;Zinc SUBCELLULAR LOCATION: Mitochondrion matrix MISCELLANEOUS: Has some ability to deacetylate histones in vitro, but seeing its subcellular location, this is unlikely in vivo." Q9NTN9,PROTEIN NAMES: Semaphorin-4G PROTEIN FAMILY: Semaphorin family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the semaphorin family. FUNCTION: Cell surface receptor for PLXNB2. May play a role in axon guidance (By similarity). KEYWORDS: Alternative splicing;Cell membrane;Developmental protein;Differentiation;Disulfide bond;Glycoprotein;Immunoglobulin domain;Membrane;Neurogenesis;Phosphoprotein;Reference proteome;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. Q9NTW7,PROTEIN NAMES: Zinc finger protein 64 (Zfp-64) (Zinc finger protein 338) PROTEIN FAMILY: Krueppel C2H2-type zinc-finger protein family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein family. FUNCTION: May be involved in the regulation of mesenchymal cell differentiation through transactivation of NOTCH1 target genes. KEYWORDS: 3D-structure;Alternative splicing;DNA-binding;Isopeptide bond;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Repeat;Transcription;Transcription regulation;Ubl conjugation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Q9NU63,"PROTEIN NAMES: Zinc finger protein 57 homolog (Zfp-57) (Zinc finger protein 698) PROTEIN FAMILY: Krueppel C2H2-type zinc-finger protein family, ZFP57 subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein family. ZFP57 subfamily. FUNCTION: Transcription regulator required to maintain maternal and paternal gene imprinting, a process by which gene expression is restricted in a parent of origin-specific manner by epigenetic modification of genomic DNA and chromatin, including DNA methylation. Acts by controlling DNA methylation during the earliest multicellular stages of development at multiple imprinting control regions (ICRs). Acts together with ZNF445, but ZNF445 seems to be the major factor in human early embryonic imprinting maintenance. In contrast, in mice, ZFP57 plays the predominant role in imprinting maintenance. Required for the establishment of maternal methylation imprints at SNRPN locus. Acts as a transcriptional repressor in Schwann cells. Binds to a 5'-TGCCGC-3' consensus sequence and recognizes the methylated CpG within this element (By similarity). KEYWORDS: Alternative splicing;Developmental protein;Diabetes mellitus;Disease variant;DNA-binding;Metal-binding;Nucleus;Reference proteome;Repeat;Repressor;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Note=Binds various differentially methylated regions (DMR). " Q9NUL5,"PROTEIN NAMES: Shiftless antiviral inhibitor of ribosomal frameshifting protein (SFL) (SHFL) (Interferon-regulated antiviral protein) (IRAV) (Repressor of yield of DENV protein) (RyDEN) PROTEIN FAMILY: SHFL family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the SHFL family. FUNCTION: Inhibits programmed -1 ribosomal frameshifting (-1PRF) of a variety of mRNAs from viruses, such as HIV1, and cellular genes, such as PEG10. Interacts with the -1PRF signal of target mRNA and translating ribosomes and causes premature translation termination at the frameshifting site. Regulates HIV1 GAG-POL expression by inhibiting -1PRF. Exhibits antiviral activity against dengue virus (DENV) and can inhibit the replication of all DENV serotypes. May block the protein translation of DENV RNA via its association with cellular mRNA-binding proteins and viral RNA. Interrupts also Zika virus replication by promoting viral NS3 degradation via a lysosome-dependent pathway. Can also limit the replication of hepatitis C virus (HCV) by restricting formation of viral replication organelle, West Nile virus (WNV), Chikungunya virus (CHIKV), herpes simplex virus type 1 (HHV-1), herpes virus type 8 (HHV-8) and human adenovirus. Binds nucleic acids with a higher affinity for ssRNA and ssDNA than for dsDNA.; FUNCTION: Isoform 4 does not inhibit programmed ribosomal frameshifting (-1PRF). Does not bind to ribosomes. KEYWORDS: Acetylation;Alternative splicing;Antiviral defense;Cytoplasm;Nucleus;Reference proteome;RNA-binding SUBCELLULAR LOCATION: Cytoplasm Nucleus Cytoplasm, P-body Note=Predominantly found in the cytoplasm. After infection, relocalizes to the DENV replication complex in perinuclear regions. " Q9NUT2,"PROTEIN NAMES: Mitochondrial potassium channel ATP-binding subunit (ATP-binding cassette sub-family B member 8, mitochondrial) (ABCB8) (Mitochondrial ATP-binding cassette 1) (M-ABC1) (Mitochondrial sulfonylurea-receptor) (MITOSUR) PROTEIN FAMILY: ABC transporter superfamily, ABCB family, Multidrug resistance exporter subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily. FUNCTION: ATP-binding subunit of the mitochondrial ATP-gated potassium channel (mitoK(ATP)). Together with pore-forming subunit CCDC51/MITOK of the mitoK(ATP) channel, mediates ATP-dependent potassium currents across the mitochondrial inner membrane. An increase in ATP intracellular levels closes the channel, inhibiting K(+) transport, whereas a decrease in ATP levels enhances K(+) uptake in the mitochondrial matrix. Plays a role in mitochondrial iron transport. Required for maintenance of normal cardiac function, possibly by influencing mitochondrial iron export and regulating the maturation of cytosolic iron sulfur cluster-containing enzymes (By similarity). KEYWORDS: 3D-structure;Alternative splicing;ATP-binding;Ion transport;Membrane;Mitochondrion;Mitochondrion inner membrane;Nucleotide-binding;Potassium;Potassium transport;Reference proteome;Transit peptide;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Mitochondrion inner membrane ; Multi-pass membrane protein " Q9NUU6,"PROTEIN NAMES: Inactive ubiquitin thioesterase OTULINL PROTEIN FAMILY: Peptidase C65 family, Otulin subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the peptidase C65 family. Otulin subfamily. FUNCTION: Lacks deubiquitinase activity. KEYWORDS: 3D-structure;Cytoplasm;Endoplasmic reticulum;Membrane;Nucleus;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Endoplasmic reticulum membrane ; Peripheral membrane protein Nucleus envelope " Q9NUV9,"PROTEIN NAMES: GTPase IMAP family member 4 (Immunity-associated nucleotide 1 protein) (IAN-1) (hIAN1) (Immunity-associated protein 4) PROTEIN FAMILY: TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily, AIG1/Toc34/Toc159-like paraseptin GTPase family, IAN subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. AIG1/Toc34/Toc159-like paraseptin GTPase family. IAN subfamily. FUNCTION: During thymocyte development, may play a role in the regulation of apoptosis (By similarity). GTPase which exhibits a higher affinity for GDP than for GTP. KEYWORDS: 3D-structure;Coiled coil;Cytoplasm;GTP-binding;Nucleotide-binding;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cytoplasm, cytosol " Q9NUY8,"PROTEIN NAMES: TBC1 domain family member 23 (HCV non-structural protein 4A-transactivated protein 1) ORGANISM: Homo sapiens (Human) FUNCTION: Putative Rab GTPase-activating protein which plays a role in vesicular trafficking. Involved in endosome-to-Golgi trafficking. Acts as a bridging protein by binding simultaneously to golgins, including GOLGA1 and GOLGA4, located at the trans-Golgi, and to the WASH complex, located on endosome-derived vesicles. Together with WDR11 complex facilitates the golgin-mediated capture of vesicles generated using AP-1. Plays a role in brain development, including in cortical neuron positioning (By similarity). May also be important for neurite outgrowth, possibly through its involvement in membrane trafficking and cargo delivery, 2 processes that are essential for axonal and dendritic growth (By similarity). May act as a general inhibitor of innate immunity signaling, strongly inhibiting multiple TLR and dectin/CLEC7A-signaling pathways. Does not alter initial activation events, but instead affects maintenance of inflammatory gene expression several hours after bacterial lipopolysaccharide (LPS) challenge (By similarity). KEYWORDS: 3D-structure;Alternative splicing;Cytoplasmic vesicle;Developmental protein;Disease variant;Golgi apparatus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Golgi apparatus, trans-Golgi network Cytoplasmic vesicle Note=Localization to the trans-Golgi is regulated by ARL1 and ARL5B/ARL8. ARL1 increases Golgi localization, while ARL5B decreases it. Recruitment to the trans-Golgi network requires the presence of GOLGA1 and GOLGA4, but not that of FAM91A1. Recruited on AP-1-derived vesicles by WDR11 complex. " Q9NVD3,"PROTEIN NAMES: SET domain-containing protein 4 PROTEIN FAMILY: Class V-like SAM-binding methyltransferase superfamily, SETD4 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the class V-like SAM-binding methyltransferase superfamily. SETD4 family. FUNCTION: Histone-lysine N-methyltransferase that acts as a regulator of cell proliferation, cell differentiation and inflammatory response. Regulates the inflammatory response by mediating mono- and dimethylation of 'Lys-4' of histone H3 (H3K4me1 and H3K4me2, respectively), leading to activate the transcription of pro-inflammatory cytokines IL6 and TNF-alpha (By similarity). Also involved in the regulation of stem cell quiescence by catalyzing the trimethylation of 'Lys-20' of histone H4 (H4K20me3), thereby promoting heterochromatin formation. Involved in proliferation, migration, paracrine and myogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) (By similarity). KEYWORDS: Alternative splicing;Cytoplasm;Inflammatory response;Methyltransferase;Nucleus;Reference proteome;S-adenosyl-L-methionine;Transferase SUBCELLULAR LOCATION: Cytoplasm, cytosol Nucleus MISCELLANEOUS: [Isoform B]: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay." Q9NVN8,"PROTEIN NAMES: Guanine nucleotide-binding protein-like 3-like protein PROTEIN FAMILY: TRAFAC class YlqF/YawG GTPase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the TRAFAC class YlqF/YawG GTPase family. FUNCTION: Stabilizes TERF1 telomeric association by preventing TERF1 recruitment by PML. Stabilizes TERF1 protein by preventing its ubiquitination and hence proteasomal degradation. Does so by interfering with TERF1-binding to FBXO4 E3 ubiquitin-protein ligase. Required for cell proliferation. By stabilizing TRF1 protein during mitosis, promotes metaphase-to-anaphase transition. Stabilizes MDM2 protein by preventing its ubiquitination, and hence proteasomal degradation. By acting on MDM2, may affect TP53 activity. Required for normal processing of ribosomal pre-rRNA. Binds GTP. KEYWORDS: Coiled coil;GTP-binding;Isopeptide bond;Nucleotide-binding;Nucleus;Reference proteome;Ribosome biogenesis;Ubl conjugation SUBCELLULAR LOCATION: Nucleus, nucleolus " Q9NVR5,"PROTEIN NAMES: Protein kintoun (Dynein assembly factor 2, axonemal) PROTEIN FAMILY: PIH1 family, Kintoun subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the PIH1 family. Kintoun subfamily. FUNCTION: Required for cytoplasmic pre-assembly of axonemal dyneins, thereby playing a central role in motility in cilia and flagella. Involved in pre-assembly of dynein arm complexes in the cytoplasm before intraflagellar transport loads them for the ciliary compartment. KEYWORDS: Alternative splicing;Ciliopathy;Cytoplasm;Kartagener syndrome;Phosphoprotein;Primary ciliary dyskinesia;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Dynein axonemal particle Note=Localizes in the apical cytoplasm around the gamma-tubulin-positive pericentriolar region, not in the cilia. MISCELLANEOUS: [Isoform 2]: May be due to exon skipping." Q9NW13,"PROTEIN NAMES: RNA-binding protein 28 (RNA-binding motif protein 28) ORGANISM: Homo sapiens (Human) FUNCTION: Nucleolar component of the spliceosomal ribonucleoprotein complexes. KEYWORDS: Acetylation;Alternative splicing;Direct protein sequencing;Disease variant;Hypotrichosis;Intellectual disability;Isopeptide bond;mRNA processing;mRNA splicing;Nucleus;Phosphoprotein;Reference proteome;Repeat;RNA-binding;Spliceosome;Ubl conjugation SUBCELLULAR LOCATION: Nucleus, nucleolus " Q9NW15,PROTEIN NAMES: Anoctamin-10 (Transmembrane protein 16K) PROTEIN FAMILY: Anoctamin family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the anoctamin family. FUNCTION: Does not exhibit calcium-activated chloride channel (CaCC) activity. Can inhibit the activity of ANO1. KEYWORDS: 3D-structure;Alternative splicing;Cell membrane;Disease variant;Membrane;Neurodegeneration;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Note=Shows predominantly an intracellular localization with a weak expression in the cell membrane. MISCELLANEOUS: The term 'anoctamin' was coined because these channels are anion selective and have eight (OCT) transmembrane segments. There is some dissatisfaction in the field with the Ano nomenclature because it is not certain that all the members of this family are anion channels or have the 8-transmembrane topology. Q9NWK9,"PROTEIN NAMES: Box C/D snoRNA protein 1 (Serologically defined breast cancer antigen NY-BR-75) (Zinc finger HIT domain-containing protein 6) PROTEIN FAMILY: BCD1 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the BCD1 family. FUNCTION: Required for box C/D snoRNAs accumulation involved in snoRNA processing, snoRNA transport to the nucleolus and ribosome biogenesis. KEYWORDS: Alternative splicing;Isopeptide bond;Metal-binding;Phosphoprotein;Reference proteome;Ribosome biogenesis;Ubl conjugation;Zinc;Zinc-finger " Q9NWZ8,"PROTEIN NAMES: Gem-associated protein 8 (Gemin-8) (Protein FAM51A1) ORGANISM: Homo sapiens (Human) FUNCTION: The SMN complex catalyzes the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome, and thereby plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP (Sm core). In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. To assemble core snRNPs, the SMN complex accepts the trapped 5Sm proteins from CLNS1A forming an intermediate. Binding of snRNA inside 5Sm triggers eviction of the SMN complex, thereby allowing binding of SNRPD3 and SNRPB to complete assembly of the core snRNP. KEYWORDS: 3D-structure;Coiled coil;Cytoplasm;mRNA processing;mRNA splicing;Nucleus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Nucleus, gem Cytoplasm Note=Found in nuclear bodies called gems (Gemini of Cajal bodies) that are often in proximity to Cajal (coiled) bodies. Also found in the cytoplasm. " Q9NX78,"PROTEIN NAMES: Protein O-mannosyl-transferase TMEM260 (Transmembrane protein 260) PROTEIN FAMILY: Glycosyltransferase 117 (GT117) family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the glycosyltransferase 117 (GT117) family. FUNCTION: O-mannosyl-transferase that transfers mannosyl residues to the hydroxyl group of serine or threonine residues of proteins. Specifically glycosylates the IPT/TIG domain of target proteins, such as MET and MST1R/RON. TMEM260-mediated O-mannosylated residues are composed of single mannose glycans that are not elongated or modified. KEYWORDS: Alternative splicing;Disease variant;Endoplasmic reticulum;Glycoprotein;Glycosyltransferase;Membrane;Reference proteome;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein.; SUBCELLULAR LOCATION: [Isoform 3]: Membrane Note=Shows perinuclear localization. " Q9NXV6,"PROTEIN NAMES: CDKN2A-interacting protein (Collaborator of ARF) PROTEIN FAMILY: CARF family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the CARF family. FUNCTION: Regulates DNA damage response in a dose-dependent manner through a number of signaling pathways involved in cell proliferation, apoptosis and senescence. KEYWORDS: Acetylation;Direct protein sequencing;Isopeptide bond;Nucleus;Phosphoprotein;Reference proteome;RNA-binding;Ubl conjugation SUBCELLULAR LOCATION: Nucleus, nucleoplasm " Q9NY97,"PROTEIN NAMES: N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase 2 (Beta-1,3-N-acetylglucosaminyltransferase 1) (BGnT-1) (Beta-1,3-Gn-T1) (Beta3Gn-T1) (Beta-1,3-galactosyltransferase 7) (Beta-1,3-GalTase 7) (Beta3Gal-T7) (Beta3GalT7) (b3Gal-T7) (Beta-3-Gx-T7) (UDP-Gal:beta-GlcNAc beta-1,3-galactosyltransferase 7) (UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2) (BGnT-2) (Beta-1,3-Gn-T2) (Beta-1,3-N-acetylglucosaminyltransferase 2) (Beta3Gn-T2) (UDP-galactose:beta-N-acetylglucosamine beta-1,3-galactosyltransferase 7) PROTEIN FAMILY: Glycosyltransferase 31 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the glycosyltransferase 31 family. FUNCTION: Beta-1,3-N-acetylglucosaminyltransferase involved in the synthesis of poly-N-acetyllactosamine. Catalyzes the initiation and elongation of poly-N-acetyllactosamine chains. Shows a marked preference for Gal(beta1-4)Glc(NAc)-based acceptors. Probably constitutes the main polylactosamine synthase. PATHWAY: Protein modification; protein glycosylation. KEYWORDS: 3D-structure;Alternative splicing;Glycoprotein;Glycosyltransferase;Golgi apparatus;Manganese;Membrane;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus membrane ; Single-pass type II membrane protein MISCELLANEOUS: [Isoform 2]: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay." Q9NYA1,"PROTEIN NAMES: Sphingosine kinase 1 (SK 1) (SPK 1) (Acetyltransferase SPHK1) ORGANISM: Homo sapiens (Human) FUNCTION: Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol. In contrast to proapoptotic SPHK2, has a negative effect on intracellular ceramide levels, enhances cell growth and inhibits apoptosis. Involved in the regulation of inflammatory response and neuroinflammation. Via the product sphingosine 1-phosphate, stimulates TRAF2 E3 ubiquitin ligase activity, and promotes activation of NF-kappa-B in response to TNF signaling leading to IL17 secretion. In response to TNF and in parallel to NF-kappa-B activation, negatively regulates RANTES induction through p38 MAPK signaling pathway. Involved in endocytic membrane trafficking induced by sphingosine, recruited to dilate endosomes, also plays a role on later stages of endosomal maturation and membrane fusion independently of its kinase activity. In Purkinje cells, seems to be also involved in the regulation of autophagosome-lysosome fusion upon VEGFA.; FUNCTION: Has serine acetyltransferase activity on PTGS2/COX2 in an acetyl-CoA dependent manner. The acetyltransferase activity increases in presence of the kinase substrate, sphingosine. During neuroinflammation, through PTGS2 acetylation, promotes neuronal secretion of specialized preresolving mediators (SPMs), especially 15-R-lipoxin A4, which results in an increase of phagocytic microglia. KEYWORDS: 3D-structure;Alternative splicing;ATP-binding;Calmodulin-binding;Cell membrane;Coated pit;Cytoplasm;Endosome;Kinase;Lipid metabolism;Membrane;Nucleotide-binding;Nucleus;Phosphoprotein;Reference proteome;Synapse;Transferase SUBCELLULAR LOCATION: Cytoplasm Nucleus Cell membrane Endosome membrane ; Peripheral membrane protein Membrane, clathrin-coated pit Synapse Note=Translocated from the cytoplasm to the plasma membrane in a CIB1-dependent manner. Binds to membranes containing negatively charged lipids but not neutral lipids. Recruited to endocytic membranes by sphingosine where promotes membrane fusion (By similarity). " Q9NZ20,"PROTEIN NAMES: Group 3 secretory phospholipase A2 (Group III secretory phospholipase A2) (GIII sPLA2) (sPLA2-III) (Phosphatidylcholine 2-acylhydrolase 3) PROTEIN FAMILY: Phospholipase A2 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the phospholipase A2 family. FUNCTION: Secretory calcium-dependent phospholipase A2 that primarily targets extracellular phospholipids. Hydrolyzes the ester bond of the fatty acyl group attached at sn-2 position of phospholipids without apparent head group selectivity. Contributes to phospholipid remodeling of low-density lipoprotein (LDL) and high-density lipoprotein (HDL) particles. Hydrolyzes LDL phospholipids releasing unsaturated fatty acids that regulate macrophage differentiation toward foam cells. May act in an autocrine and paracrine manner. Secreted by immature mast cells, acts on nearby fibroblasts upstream to PTDGS to synthesize prostaglandin D2 (PGD2), which in turn promotes mast cell maturation and degranulation via PTGDR. Secreted by epididymal epithelium, acts on immature sperm cells within the duct, modulating the degree of unsaturation of the fatty acyl components of phosphatidylcholines required for acrosome assembly and sperm cell motility. Facilitates the replacement of fatty acyl chains in phosphatidylcholines in sperm membranes from omega-6 and omega-9 to omega-3 polyunsaturated fatty acids (PUFAs). Coupled to lipoxygenase pathway, may process omega-6 PUFAs to generate oxygenated lipid mediators in the male reproductive tract (By similarity). At pericentrosomal preciliary compartment, negatively regulates ciliogenesis likely by regulating endocytotic recycling of ciliary membrane protein. Coupled to cyclooxygenase pathway provides arachidonate to generate prostaglandin E2 (PGE2), a potent immunomodulatory lipid in inflammation and tumorigenesis. At colonic epithelial barrier, preferentially hydrolyzes phospholipids having arachidonate and docosahexaenoate at sn-2 position, contributing to the generation of oxygenated metabolites involved in colonic stem cell homeostasis. Releases C16:0 and C18:0 lysophosphatidylcholine subclasses from neuron plasma membranes and promotes neurite outgrowth and neuron survival. KEYWORDS: Calcium;Cell membrane;Cilium biogenesis/degradation;Cytoplasm;Cytoskeleton;Disulfide bond;Endosome;Glycoprotein;Hydrolase;Lipid metabolism;Mast cell degranulation;Membrane;Metal-binding;Phospholipid metabolism;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted Cell membrane Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole Recycling endosome Note=Localized at pericentrosomal preciliary compartment. " Q9NZD1,PROTEIN NAMES: G-protein coupled receptor family C group 5 member D PROTEIN FAMILY: G-protein coupled receptor 3 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the G-protein coupled receptor 3 family. KEYWORDS: Alternative splicing;Cell membrane;G-protein coupled receptor;Membrane;Receptor;Reference proteome;Transducer;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Q9NZV7,PROTEIN NAMES: Zinc finger imprinted 2 (Zinc finger protein 656) PROTEIN FAMILY: Krueppel C2H2-type zinc-finger protein family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein family. FUNCTION: May be involved in transcriptional regulation. KEYWORDS: DNA-binding;Metal-binding;Nucleus;Reference proteome;Repeat;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Q9P0G3,"PROTEIN NAMES: Kallikrein-14 (hK14) (Kallikrein-like protein 6) (KLK-L6) PROTEIN FAMILY: Peptidase S1 family, Kallikrein subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the peptidase S1 family. Kallikrein subfamily. FUNCTION: Serine-type endopeptidase with a dual trypsin-like and chymotrypsin-like substrate specificity. May activate/inactivate the proteinase-activated receptors F2R, F2RL1 and F2RL3 and other kallikreins including KLK1, KLK3, KLK5 and KLK11. May function in seminal clot liquefaction through direct cleavage of the semenogelin SEMG1 and SEMG2 and activation of KLK3. May function through desmoglein DSG1 cleavage in epidermal desquamation a process by which the most superficial corneocytes are shed from the skin surface. May be involved in several aspects of tumor progression including growth, invasion and angiogenesis. KEYWORDS: Autocatalytic cleavage;Direct protein sequencing;Disulfide bond;Hydrolase;Protease;Reference proteome;Secreted;Serine protease;Signal;Zymogen SUBCELLULAR LOCATION: Secreted, extracellular space " Q9P0K8,"PROTEIN NAMES: Forkhead box protein J2 (Fork head homologous X) ORGANISM: Homo sapiens (Human) FUNCTION: [Isoform FOXJ2.L]: Transcriptional activator. Able to bind to two different type of DNA binding sites. More effective than isoform FOXJ2.S in transcriptional activation. Plays an important role in spermatogenesis, especially in spermatocyte meiosis (By similarity).; FUNCTION: [Isoform FOXJ2.S]: Transcriptional activator. KEYWORDS: Acetylation;Activator;Alternative splicing;Differentiation;DNA-binding;Meiosis;Nucleus;Phosphoprotein;Reference proteome;Spermatogenesis;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus. " Q9P0K9,PROTEIN NAMES: DOMON domain-containing protein FRRS1L (Brain protein CG-6) (Ferric-chelate reductase 1-like protein) ORGANISM: Homo sapiens (Human) FUNCTION: Important modulator of glutamate signaling pathway. KEYWORDS: Cell membrane;Disease variant;Epilepsy;Membrane;Reference proteome;Signal;Synapse;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane Synapse Q9P0W8,"PROTEIN NAMES: Spermatogenesis-associated protein 7 (HSD-3.1) (Spermatogenesis-associated protein HSD3) ORGANISM: Homo sapiens (Human) FUNCTION: Involved in the maintenance of both rod and cone photoreceptor cells (By similarity). It is required for recruitment and proper localization of RPGRIP1 to the photoreceptor connecting cilium (CC), as well as photoreceptor-specific localization of proximal CC proteins at the distal CC (By similarity). Maintenance of protein localization at the photoreceptor-specific distal CC is essential for normal microtubule stability and to prevent photoreceptor degeneration (By similarity). KEYWORDS: Alternative splicing;Cell projection;Cytoplasm;Cytoskeleton;Leber congenital amaurosis;Reference proteome;Retinitis pigmentosa;Sensory transduction;Vision SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, cilium axoneme Cytoplasm, cytoskeleton, cilium basal body Cytoplasm, cytoskeleton Cell projection, cilium, photoreceptor outer segment Note=Localizes to the microtubule network. " Q9P1W3,PROTEIN NAMES: Calcium permeable stress-gated cation channel 1 (Transmembrane protein 63C) PROTEIN FAMILY: CSC1 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the CSC1 (TC 1.A.17) family. FUNCTION: Acts as an osmosensitive calcium-permeable cation channel. Required for the functional integrity of the kidney glomerular filtration barrier (By similarity). KEYWORDS: Calcium;Cell membrane;Disease variant;Hereditary spastic paraplegia;Ion channel;Ion transport;Membrane;Neurodegeneration;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Q9P209,"PROTEIN NAMES: Centrosomal protein of 72 kDa (Cep72) PROTEIN FAMILY: CEP72 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the CEP72 family. FUNCTION: Involved in the recruitment of key centrosomal proteins to the centrosome. Provides centrosomal microtubule-nucleation activity on the gamma-tubulin ring complexes (gamma-TuRCs) and has critical roles in forming a focused bipolar spindle, which is needed for proper tension generation between sister chromatids. Required for localization of KIZ, AKAP9 and gamma-tubulin ring complexes (gamma-TuRCs). Involved in centriole duplication. Required for CDK5RAP22, CEP152, WDR62 and CEP63 centrosomal localization and promotes the centrosomal localization of CDK2. KEYWORDS: Alternative splicing;Coiled coil;Cytoplasm;Cytoskeleton;Leucine-rich repeat;Phosphoprotein;Reference proteome;Repeat SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriolar satellite Note=Localizes to the centrosome and centrosome-surrounding particles throughout the cell cycle. These particles disappear after microtubules are depolymerized using nocodazole, suggesting that CEP72-associating particles localize in a microtubule- dependent manner. " Q9P2F9,PROTEIN NAMES: Zinc finger protein 319 PROTEIN FAMILY: Krueppel C2H2-type zinc-finger protein family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein family. FUNCTION: May be involved in transcriptional regulation. KEYWORDS: DNA-binding;Isopeptide bond;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Repeat;Transcription;Transcription regulation;Ubl conjugation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Q9P2X3,"PROTEIN NAMES: Protein IMPACT (Imprinted and ancient gene protein homolog) PROTEIN FAMILY: IMPACT family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the IMPACT family. FUNCTION: Translational regulator that ensures constant high levels of translation upon a variety of stress conditions, such as amino acid starvation, UV-C irradiation, proteasome inhibitor treatment and glucose deprivation. Plays a role as a negative regulator of the EIF2AK4/GCN2 kinase activity; impairs GCN1-mediated EIF2AK4/GCN2 activation, and hence EIF2AK4/GCN2-mediated eIF-2-alpha phosphorylation and subsequent down-regulation of protein synthesis. May be required to regulate translation in specific neuronal cells under amino acid starvation conditions by preventing GCN2 activation and therefore ATF4 synthesis. Through its inhibitory action on EIF2AK4/GCN2, plays a role in differentiation of neuronal cells by stimulating neurite outgrowth. KEYWORDS: Actin-binding;Alternative splicing;Cytoplasm;Differentiation;Neurogenesis;Phosphoprotein;Reference proteome;Repressor;Stress response;Translation regulation SUBCELLULAR LOCATION: Cytoplasm MISCELLANEOUS: In contrast to the mouse or rabbit ortholog, the IMPACT locus is not imprinted in human." Q9P371,PROTEIN NAMES: AMSH-like protease sst2 (Suppressor of ste12 deletion protein 2) PROTEIN FAMILY: Peptidase M67C family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the peptidase M67C family. FUNCTION: Zinc metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. Does not cleave 'Lys-48'-linked polyubiquitin chains (By similarity). Plays a role in the multivesicular body (MVB) sorting pathway. Required for ubiquitin-dependent sorting of proteins into the endosome and subsequent trafficking to the vacuole. May regulate MVB sorting through deubiquitination of ubiquitinated ESCRT proteins. KEYWORDS: 3D-structure;Cytoplasm;Endosome;Hydrolase;Metal-binding;Metalloprotease;Phosphoprotein;Protease;Reference proteome;Ubl conjugation pathway;Zinc SUBCELLULAR LOCATION: Cytoplasm Endosome Q9P376,PROTEIN NAMES: RNA polymerase II subunit A C-terminal domain phosphatase (CTD phosphatase fcp1) ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) FUNCTION: Processively dephosphorylates 'Ser-2' and 'Ser-5' of the heptad repeats YSPTSPS in the C-terminal domain of the largest RNA polymerase II subunit. This promotes the activity of RNA polymerase II. KEYWORDS: 3D-structure;Cobalt;Hydrolase;Magnesium;Manganese;Metal-binding;Nucleus;Protein phosphatase;Reference proteome SUBCELLULAR LOCATION: Nucleus Q9P6I7,"PROTEIN NAMES: Adenine deaminase (ADE) (Adenine aminohydrolase) (AAH) PROTEIN FAMILY: Metallo-dependent hydrolases superfamily, Adenosine and AMP deaminases family, Adenine deaminase type 2 subfamily ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the metallo-dependent hydrolases superfamily. Adenosine and AMP deaminases family. Adenine deaminase type 2 subfamily. FUNCTION: Catalyzes the hydrolytic deamination of adenine to hypoxanthine. Plays an important role in the purine salvage pathway and in nitrogen catabolism. Also exhibits a low activity towards N(6)-substituted adenines that are commonly known as the plant hormones cytokinins. KEYWORDS: Cytoplasm;Hydrolase;Metal-binding;Nucleotide metabolism;Nucleus;Reference proteome;Zinc SUBCELLULAR LOCATION: Cytoplasm Nucleus " Q9P6N1,"PROTEIN NAMES: Autophagy-related protein 21 (Meiotically up-regulated gene 179 protein) PROTEIN FAMILY: WD repeat PROPPIN family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the WD repeat PROPPIN family. FUNCTION: Required for cytoplasm to vacuole transport (Cvt) vesicles formation and autophagy (By similarity). Has a role in sporulation. KEYWORDS: Autophagy;Cytoplasm;Golgi apparatus;Membrane;Protein transport;Reference proteome;Repeat;Sporulation;Transport;Vacuole;WD repeat SUBCELLULAR LOCATION: Cytoplasm. Golgi apparatus, Golgi stack membrane; Peripheral membrane protein. Vacuole membrane; Peripheral membrane protein. Preautophagosomal structure membrane; Peripheral membrane protein. " Q9P7C3,"PROTEIN NAMES: Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase (ER alpha-1,2-mannosidase) (Man(9)-alpha-mannosidase) PROTEIN FAMILY: Glycosyl hydrolase 47 family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the glycosyl hydrolase 47 family. FUNCTION: Involved in glycoprotein quality control as it is important for the targeting of misfolded glycoproteins for degradation. It trims a single alpha-1,2-linked mannose residue from Man(9)GlcNAc(2) to produce Man(8)GlcNAc(2) with low efficiency. PATHWAY: Protein modification; protein glycosylation. KEYWORDS: Calcium;Disulfide bond;Glycoprotein;Glycosidase;Hydrolase;Membrane;Metal-binding;Reference proteome;Signal-anchor;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Single-pass type II membrane protein " Q9P7V9,PROTEIN NAMES: Probable ubiquitin carboxyl-terminal hydrolase 9 (Deubiquitinating enzyme 9) (Ubiquitin thioesterase 9) (Ubiquitin-specific-processing protease 9) PROTEIN FAMILY: Peptidase C19 family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the peptidase C19 family. FUNCTION: Ubiquitin C-terminal hydrolase involved in regulating actin dynamics and/or endocytosis at cell tips and septa. KEYWORDS: Cytoplasm;Hydrolase;Nucleus;Phosphoprotein;Protease;Reference proteome;Thiol protease;Ubl conjugation pathway SUBCELLULAR LOCATION: Nucleus Cytoplasm Cell tip Q9P7Y8,"PROTEIN NAMES: Septin ring organizing protein mid2 PROTEIN FAMILY: BUD4 family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the BUD4 family. FUNCTION: Responsible for the proper stability and function of septins during cytokinesis. Required for the correct formation of the medial septin ring structure in mitosis and for the proper localization of endo-glucanases agn1 and eng1, which are needed for efficient cell separation. May act as a landmark for the localization of hydrolytic proteins to the medial region. KEYWORDS: Cell cycle;Cell division;Cytoplasm;Cytoskeleton;Mitosis;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cytoplasm, cell cortex Cytoplasm, cytoskeleton Note=Localizes to the medial ring at the cell cortex of dividing cells. Initially forms a single ring, which subsequently splits into two distinct rings as the septum forms, and disappears as cells separate. Requires septins for proper localization. " Q9PTN2,"PROTEIN NAMES: Vitamin D3 receptor A (VDR-A) (1,25-dihydroxyvitamin D3 receptor A) (Nuclear receptor subfamily 1 group I member 1-A) PROTEIN FAMILY: Nuclear hormone receptor family, NR1 subfamily ORGANISM: Danio rerio (Zebrafish) (Brachydanio rerio) SIMILARITY: Belongs to the nuclear hormone receptor family. NR1 subfamily. FUNCTION: Nuclear receptor for calcitriol, the active form of vitamin D3 which mediates the action of this vitamin on cells. Enters the nucleus upon vitamin D3 binding where it forms heterodimers with the retinoid X receptor/RXR. The VDR-RXR heterodimers bind to specific response elements on DNA and activate the transcription of vitamin D3-responsive target genes. Recruited to promoters via its interaction with BAZ1B/WSTF which mediates the interaction with acetylated histones, an essential step for VDR-promoter association. Plays a central role in calcium homeostasis. KEYWORDS: 3D-structure;Cytoplasm;DNA-binding;Metal-binding;Nucleus;Receptor;Reference proteome;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Cytoplasm Note=Localizes mainly to the nucleus. Translocated into the nucleus via both ligand-dependent and ligand-independent pathways; ligand-independent nuclear translocation is mediated by IPO4. " Q9PTT2,PROTEIN NAMES: Jeltraxin (Egg jelly pentraxin) PROTEIN FAMILY: Pentraxin family ORGANISM: Lepidobatrachus laevis (Budgett's frog) SIMILARITY: Belongs to the pentraxin family. FUNCTION: Calcium-dependent beta-galactose specific lectin. KEYWORDS: Calcium;Direct protein sequencing;Disulfide bond;Glycoprotein;Lectin;Metal-binding;Secreted;Signal SUBCELLULAR LOCATION: Secreted Q9PUQ1,"PROTEIN NAMES: ETS translocation variant 4 (Polyomavirus enhancer activator 3 homolog) (Protein PEA3) PROTEIN FAMILY: ETS family ORGANISM: Danio rerio (Zebrafish) (Brachydanio rerio) SIMILARITY: Belongs to the ETS family. FUNCTION: Transcriptional activator that binds to the (5'-CCGGA[AT]-3') motif. May control the acquisition of specific cell fates at an early stage during development of the somites and nervous system. May mediate the cellular effects of the fibroblast growth factors on embryogenesis. KEYWORDS: Activator;DNA-binding;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: Transcriptional activation activity is enhanced by Raf1, ERK and PKA." Q9QWG5,"PROTEIN NAMES: Type II inositol 3,4-bisphosphate 4-phosphatase (Inositol polyphosphate 4-phosphatase type II) PROTEIN FAMILY: Inositol 3,4-bisphosphate 4-phosphatase family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the inositol 3,4-bisphosphate 4-phosphatase family. FUNCTION: Catalyzes the hydrolysis of the 4-position phosphate of phosphatidylinositol 3,4-bisphosphate, inositol 1,3,4-trisphosphate and inositol 3,4-bisphosphate. Plays a role in the late stages of macropinocytosis by dephosphorylating phosphatidylinositol 3,4-bisphosphate in membrane ruffles (By similarity). The lipid phosphatase activity is critical for tumor suppressor function. Antagonizes the PI3K-AKT/PKB signaling pathway by dephosphorylating phosphoinositides and thereby modulating cell cycle progression and cell survival (By similarity). PATHWAY: Signal transduction; phosphatidylinositol signaling pathway. KEYWORDS: Alternative splicing;Hydrolase;Lipid metabolism;Reference proteome MISCELLANEOUS: [Isoform 2]: Inactive." Q9QXK2,"PROTEIN NAMES: E3 ubiquitin-protein ligase RAD18 (Postreplication repair protein RAD18) (mRAD18Sc) (RING-type E3 ubiquitin transferase RAD18) PROTEIN FAMILY: RAD18 family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the RAD18 family. FUNCTION: E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. Has ssDNA binding activity. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Acetylation;Cytoplasm;Cytoskeleton;DNA damage;DNA repair;DNA-binding;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Transferase;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Note=Associates with chromatin. Colocalizes with SLF1 in the nucleus and to centrosomes. Relocalizes with SLF1 to nuclear foci in response to DNA damage. Accumulates with the SLF1-SLF2 and SMC5-SMC6 complexes at replication-coupled DNA interstrand repair and DNA double-strand breaks (DSBs) sites on chromatin in a ubiquitin-dependent manner. " Q9QXL7,"PROTEIN NAMES: Nucleoside diphosphate kinase homolog 7 (NDK 7) (NDP kinase homolog 7) (3'-5' exonuclease NME7) (Protein kinase NME7) (nm23-H7) PROTEIN FAMILY: NDK family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the NDK family. FUNCTION: Possesses an intrinsic kinase activity. Displays 3'-5' exonuclease activity with a preference for single-stranded DNA. Does not seem to have nucleoside diphosphate kinase activity. Functional component of the gamma-tubulin ring complex, implicated in the regulation of the microtubule-nucleating activity of the gamma-tubulin ring complex in centrosomes, in a kinase activity-dependent manner. Microtubule inner protein (MIP) part of the dynein-decorated doublet microtubules (DMTs) in cilia axoneme, which is required for motile cilia beating. Functionnel component of the gamma-tubulin ring complex, implicated in the regulation of the microtubule-nucleating activity of the gamma-tubulin ring complex in centrosomes, in a kinase activity-dependent manner. KEYWORDS: Cell projection;Cilium;Cytoplasm;Cytoskeleton;Flagellum;Hydrolase;Kinase;Nucleus;Reference proteome;Transferase SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Nucleus Cytoplasm Cytoplasm, cytoskeleton, spindle Cytoplasm, cytoskeleton, cilium axoneme Cytoplasm, cytoskeleton, flagellum axoneme Note=Localizes to centrosomes through its assembly into gamma-tubulin ring complex. The centrosomal content of NME7 varies during the cell cycle, being highest in mitosis and lowest in early G1. " Q9QYJ6,"PROTEIN NAMES: cAMP and cAMP-inhibited cGMP 3',5'-cyclic phosphodiesterase 10A PROTEIN FAMILY: Cyclic nucleotide phosphodiesterase family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the cyclic nucleotide phosphodiesterase family. FUNCTION: Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. Can hydrolyze both cAMP and cGMP, but has higher affinity for cAMP and is more efficient with cAMP as substrate. PATHWAY: Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.; PATHWAY: Purine metabolism; 3',5'-cyclic GMP degradation; GMP from 3',5'-cyclic GMP: step 1/1. KEYWORDS: 3D-structure;Allosteric enzyme;Alternative splicing;cAMP;cAMP-binding;cGMP;cGMP-binding;Cytoplasm;Hydrolase;Metal-binding;Nucleotide-binding;Reference proteome;Repeat SUBCELLULAR LOCATION: Cytoplasm, cytosol " Q9QYY0,PROTEIN NAMES: GRB2-associated-binding protein 1 (GRB2-associated binder 1) (Growth factor receptor bound protein 2-associated protein 1) PROTEIN FAMILY: GAB family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the GAB family. FUNCTION: Adapter protein that plays a role in intracellular signaling cascades triggered by activated receptor-type kinases. Plays a role in FGFR1 signaling. Probably involved in signaling by the epidermal growth factor receptor (EGFR) and the insulin receptor (INSR). Involved in the MET/HGF-signaling pathway. KEYWORDS: Acetylation;Phosphoprotein;Reference proteome Q9QZ85,"PROTEIN NAMES: Interferon-inducible GTPase 1 PROTEIN FAMILY: TRAFAC class dynamin-like GTPase superfamily, IRG family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the TRAFAC class dynamin-like GTPase superfamily. IRG family. FUNCTION: GTPase with low activity. Has higher affinity for GDP than for GTP. Plays a role in resistance to intracellular pathogens. Required for disruption of the parasitophorous vacuole formed following T.gondii infection and subsequent killing of the parasite. Mediates resistance to C.trachomatis infection by targeting bacterial inclusions to autophagosomes for subsequent lysosomal destruction. KEYWORDS: 3D-structure;Cytoplasm;Endoplasmic reticulum;Golgi apparatus;GTP-binding;Hydrolase;Immunity;Innate immunity;Lipoprotein;Membrane;Myristate;Nucleotide-binding;Nucleus;Reference proteome SUBCELLULAR LOCATION: Cytoplasm. Nucleus membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein. Golgi apparatus, Golgi stack membrane; Peripheral membrane protein. Parasitophorous vacuole membrane. Note=Localizes to the bacterial inclusions formed following C.trachomatis infection. Accumulates in a GTP-bound form on the parasitophorous vacuole membranes formed following T.gondii infection but exists in a GDP-bound form in uninfected cells. " Q9QZE2,"PROTEIN NAMES: Cytokine-dependent hematopoietic cell linker (Mast cell immunoreceptor signal transducer) ORGANISM: Mus musculus (Mouse) FUNCTION: An adapter protein which plays a role in the regulation of immunoreceptor signaling, including PLC-gamma-mediated B-cell antigen receptor (BCR) signaling and FC-epsilon R1-mediated mast cell degranulation. Together with FGR, it acts as a negative regulator of natural killer cell-activating receptors and inhibits interferon-gamma production. Acts as a positive regulator of both T-cell receptor and natural killer T (NKT) cell receptor signaling in CD4-positive NKT cells. Together with MAP4K1, it enhances CD3-triggered activation of T-cells and subsequent IL2 production. May be involved in tumor necrosis factor induced cell death by promoting reactive oxidative species generation, and MLKL oligomerization, ultimately leading to necrosis. Involved in phosphorylation of LAT. May be involved in high affinity immunoglobulin epsilon receptor signaling in mast cells. KEYWORDS: Alternative splicing;Cytoplasm;Phosphoprotein;Reference proteome;SH2 domain SUBCELLULAR LOCATION: Cytoplasm " Q9QZL6,"PROTEIN NAMES: Ubiquitin carboxyl-terminal hydrolase 21 (Deubiquitinating enzyme 21) (Ubiquitin thioesterase 21) (Ubiquitin-specific-processing protease 21) PROTEIN FAMILY: Peptidase C19 family, USP21 subfamily ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the peptidase C19 family. USP21 subfamily. FUNCTION: Deubiquitinates histone H2A, a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A releaves the repression of di- and trimethylation of histone H3 at 'Lys-4', resulting in regulation of transcriptional initiation. Regulates gene expression via histone H2A deubiquitination. Deubiquitinates BAZ2A/TIP5 leading to its stabilization (By similarity). Also capable of removing NEDD8 from NEDD8 conjugates but has no effect on Sentrin-1 conjugates (By similarity). Also acts as a negative regulator of the ribosome quality control (RQC) by mediating deubiquitination of 40S ribosomal proteins RPS10/eS10 and RPS20/uS10, thereby antagonizing ZNF598-mediated 40S ubiquitination (By similarity). KEYWORDS: Activator;Chromatin regulator;Cytoplasm;Hydrolase;Metal-binding;Nucleus;Protease;Reference proteome;Thiol protease;Transcription;Transcription regulation;Ubl conjugation pathway;Zinc SUBCELLULAR LOCATION: Cytoplasm Nucleus " Q9QZM4,PROTEIN NAMES: Tumor necrosis factor receptor superfamily member 10B (Death receptor 5) (MK) (CD antigen CD262) ORGANISM: Mus musculus (Mouse) FUNCTION: Receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF-kappa-B. Essential for ER stress-induced apoptosis. KEYWORDS: Apoptosis;Disulfide bond;Glycoprotein;Membrane;Receptor;Reference proteome;Repeat;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. Q9QZN1,"PROTEIN NAMES: F-box/LRR-repeat protein 17 (F-box and leucine-rich repeat protein 17) (F-box only protein 13) PROTEIN FAMILY: FBXL17 family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the FBXL17 family. FUNCTION: Substrate-recognition component of the SCF(FBXL17) E3 ubiquitin ligase complex, a key component of a quality control pathway required to ensure functional dimerization of BTB domain-containing proteins (dimerization quality control, DQC). FBXL17 specifically recognizes and binds a conserved degron of non-consecutive residues present at the interface of BTB dimers of aberrant composition: aberrant BTB dimer are then ubiquitinated by the SCF(FBXL17) complex and degraded by the proteasome (By similarity). The ability of the SCF(FBXL17) complex to eliminate compromised BTB dimers is required for the differentiation and survival of neural crest and neuronal cells (By similarity). The SCF(FBXL17) complex mediates ubiquitination and degradation of BACH1 (By similarity). The SCF(FBXL17) complex is also involved in the regulation of the hedgehog/smoothened (Hh) signaling pathway by mediating the ubiquitination and degradation of SUFU, allowing the release of GLI1 from SUFU for proper Hh signal transduction. The SCF(FBXL17) complex mediates ubiquitination and degradation of PRMT1. KEYWORDS: Alternative splicing;Cytoplasm;Nucleus;Reference proteome;Ubl conjugation pathway SUBCELLULAR LOCATION: Cytoplasm Nucleus Note=Present in the cytoplasm and nucleus; more abundant in the cytoplasm. " Q9QZS3,"PROTEIN NAMES: Protein numb homolog (m-Nb) (m-Numb) ORGANISM: Mus musculus (Mouse) FUNCTION: Regulates clathrin-mediated receptor endocytosis (By similarity). Plays a role in the process of neurogenesis. Required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate. Not required for the proliferation of neural progenitor cells before the onset of neurogenesis. Also involved postnatally in the subventricular zone (SVZ) neurogenesis by regulating SVZ neuroblasts survival and ependymal wall integrity. May also mediate local repair of brain ventricular wall damage. KEYWORDS: 3D-structure;Alternative splicing;Cell membrane;Developmental protein;Endosome;Membrane;Neurogenesis;Phosphoprotein;Reference proteome;Ubl conjugation SUBCELLULAR LOCATION: Cell membrane ; Peripheral membrane protein ; Cytoplasmic side Endosome membrane ; Peripheral membrane protein ; Cytoplasmic side Note=Localizes to perinuclear endosomes in an AAK1-dependent manner. " Q9R0A5,"PROTEIN NAMES: Serine/threonine-protein kinase Nek3 (Never in mitosis A-related kinase 3) (NimA-related protein kinase 3) PROTEIN FAMILY: Protein kinase superfamily, NEK Ser/Thr protein kinase family, NIMA subfamily ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily. FUNCTION: Protein kinase which influences neuronal morphogenesis and polarity through effects on microtubules. Regulates microtubule acetylation in neurons. Contributes to prolactin-mediated phosphorylation of PXN and VAV2. KEYWORDS: Acetylation;ATP-binding;Cell cycle;Cell division;Cell projection;Cytoplasm;Kinase;Magnesium;Metal-binding;Mitosis;Nucleotide-binding;Phosphoprotein;Reference proteome;Serine/threonine-protein kinase;Transferase SUBCELLULAR LOCATION: Cytoplasm Cell projection, axon " Q9R1K7,"PROTEIN NAMES: Tubulin delta chain (Delta-tubulin) PROTEIN FAMILY: Tubulin family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the tubulin family. FUNCTION: Acts as a positive regulator of hedgehog signaling and regulates ciliary function. KEYWORDS: Cell projection;Cilium biogenesis/degradation;Cytoplasm;Cytoskeleton;Developmental protein;GTP-binding;Microtubule;Nucleotide-binding;Nucleus;Reference proteome SUBCELLULAR LOCATION: Cell projection, cilium Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole Cytoplasm Nucleus Note=Associated with centrioles. Both cytoplasmic and nuclear. In the elongating spermatid it is associated with the manchette, a specialized microtubule system present during reshaping of the sperm head. " Q9R233,PROTEIN NAMES: Tapasin (TPN) (TPSN) (TAP-associated protein) (TAP-binding protein) ORGANISM: Mus musculus (Mouse) FUNCTION: Involved in the association of MHC class I with transporter associated with antigen processing (TAP) and in the assembly of MHC class I with peptide (peptide loading). KEYWORDS: 3D-structure;Alternative splicing;Disulfide bond;Endoplasmic reticulum;Glycoprotein;Immunoglobulin domain;Membrane;Reference proteome;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Single-pass type I membrane protein Q9RBS0,PROTEIN NAMES: Protein PopA1 [Cleaved into: Protein PopA2; Protein PopA3] ORGANISM: Ralstonia nicotianae (strain GMI1000) (Ralstonia solanacearum) FUNCTION: Acts as a specific hypersensitive response (HR) elicitor. Has activity on tobacco (non-host plant) and petunia but is without activity on tomato (host plant); PopA3 seems to be more active than a PopA1-PopA2 mixture. KEYWORDS: Direct protein sequencing;Hypersensitive response elicitation;Plasmid;Reference proteome;Secreted SUBCELLULAR LOCATION: Secreted Note=Probably secreted via a type III secretion system (T3SS). Q9RC92,"PROTEIN NAMES: Unsaturated glucuronyl hydrolase (UGL) (Glycosaminoglycan hydrolase) (Glycuronidase) (Unsaturated uronic acid hydrolase) PROTEIN FAMILY: Glycosyl hydrolase 88 family ORGANISM: Bacillus sp. (strain GL1) SIMILARITY: Belongs to the glycosyl hydrolase 88 family. FUNCTION: Catalyzes the hydrolysis of oligosaccharides with unsaturated glucuronyl residues at the non-reducing terminal, to a sugar or an amino sugar, and an unsaturated D-glucuronic acid (GlcA), which is nonenzymatically converted immediately to alpha-keto acid. KEYWORDS: 3D-structure;Carbohydrate metabolism;Cytoplasm;Direct protein sequencing;Glycosidase;Hydrolase;Polysaccharide degradation SUBCELLULAR LOCATION: Cytoplasm. " Q9RCG0,"PROTEIN NAMES: Methanol:N,N-dimethyl-4-nitrosoaniline oxidoreductase (MNO) (Methanol dehydrogenase (nicotinoprotein)) (Methanol:NDMA oxidoreductase) PROTEIN FAMILY: Iron-containing alcohol dehydrogenase family ORGANISM: Amycolatopsis methanolica SIMILARITY: Belongs to the iron-containing alcohol dehydrogenase family. FUNCTION: Catalyzes the oxidation of methanol to yield formaldehyde. While the in vivo electron acceptor is not known, N,N-dimethyl-4-nitrosoaniline (NDMA) can serve this function in vitro and is reduced to 4-(hydroxylamino)-N,N-dimethylaniline. It can also use various other primary alcohols, polyols and formaldehyde. In addition, MNO is able to produce methylformate from methanol plus formaldehyde, and possesses a formaldehyde dismutase and a NADH-dependent formaldehyde reductase activity. KEYWORDS: Direct protein sequencing;Magnesium;Methanol utilization;NADP;Oxidoreductase;Zinc " Q9RGX8,"PROTEIN NAMES: Beta-agarase B PROTEIN FAMILY: Glycosyl hydrolase 16 family ORGANISM: Zobellia galactanivorans (strain DSM 12802 / CCUG 47099 / CIP 106680 / NCIMB 13871 / Dsij) SIMILARITY: Belongs to the glycosyl hydrolase 16 family. FUNCTION: Cleaves the beta-1,4-linkages between beta-D-galactose and alpha-L-3,6-anhydro-galactose residues in agarose. Cleaves agarose in a random manner with retention of the anomeric-bond configuration, producing beta-anomers that give rise progressively to alpha-anomers when mutarotation takes place. Also tolerant to hybrid substrates containing C6-sulfate groups at the -4, +1, and +3 positions. KEYWORDS: 3D-structure;Cell outer membrane;Glycosidase;Hydrolase;Lipoprotein;Membrane;Palmitate;Reference proteome;Signal SUBCELLULAR LOCATION: Cell outer membrane ; Lipid-anchor " Q9RRH3,"PROTEIN NAMES: DNA damage-responsive serine/threonine-protein kinase RqkA (Radiation and pyrroloquinoline quinone inducible protein kinase) PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family ORGANISM: Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / CCUG 27074 / LMG 4051 / NBRC 15346 / NCIMB 9279 / VKM B-1422 / R1) SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. FUNCTION: Plays an important role in radiation resistance and DNA double-strand break (DSB) repair. Involved in transcriptional regulation of genes important for bacterial stress response. Phosphorylates PprA in vitro. KEYWORDS: ATP-binding;Kinase;Nucleotide-binding;Phosphoprotein;Reference proteome;Serine/threonine-protein kinase;Stress response;Transferase " Q9RY80,PROTEIN NAMES: Single-stranded DNA-binding protein DdrB (DNA damage response protein B) ORGANISM: Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / CCUG 27074 / LMG 4051 / NBRC 15346 / NCIMB 9279 / VKM B-1422 / R1) FUNCTION: ssDNA-binding protein that contributes to the ionizing radiation resistance of D.radiodurans. Plays a role in DNA repair and genome reconstitution in a RecA-independent process. Required for recovery from severe genomic fragmentation as a result of exposure to severe levels of ionizing radiation. Binds ssDNA but not dsDNA. Stimulates annealing of complementary ssDNA. Does not complement an ssb disruption. KEYWORDS: 3D-structure;DNA damage;DNA repair;DNA-binding;Reference proteome;Stress response Q9RYE6,"PROTEIN NAMES: DNA damage response protein C ORGANISM: Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / CCUG 27074 / LMG 4051 / NBRC 15346 / NCIMB 9279 / VKM B-1422 / R1) FUNCTION: Appears to contribute to D.radiodurans capacity to survive exposure to ionizing radiation. Likely functions as a DNA damage-induced nucleoid-associated protein (NAP) that contributes to the enhanced level of nucleoid compaction after irradiation by bridging DNA duplexes, thereby limiting the dispersion of the fragmented genome immediately after irradiation to facilitate subsequent DNA repair. In vitro, binds both ssDNA and dsDNA, and is able to compact circular DNA, circularize linear DNA, anneal complementary DNA strands and protect DNA from nucleases. KEYWORDS: 3D-structure;Cytoplasm;DNA damage;DNA repair;Reference proteome;Stress response SUBCELLULAR LOCATION: Cytoplasm, nucleoid. Note=Is distributed all over the nucleoid shortly after irradiation, but after 2-3 hours, it forms discrete foci located at the sites of septal closure in between the newly segregated chromosomes of D.radiodurans. " Q9S3V1,"PROTEIN NAMES: Flavin-dependent L-tryptophan oxidase VioA PROTEIN FAMILY: Flavin monoamine oxidase family ORGANISM: Chromobacterium violaceum (strain ATCC 12472 / DSM 30191 / JCM 1249 / NBRC 12614 / NCIMB 9131 / NCTC 9757) SIMILARITY: Belongs to the flavin monoamine oxidase family. FUNCTION: The enzyme generates the imine form of indole 3-pyruvate (IPA) from L-tryptophan (L-Trp), with concomitant two-electron reduction of O(2) to H(2)O(2). PATHWAY: Pigment biosynthesis; violacein biosynthesis. KEYWORDS: 3D-structure;Antibiotic biosynthesis;FAD;Flavoprotein;Magnesium;Metal-binding;Oxidoreductase;Reference proteome " Q9S4K6,PROTEIN NAMES: Autotransporter heptosyltransferase TibC PROTEIN FAMILY: Glycosyltransferase 9 family ORGANISM: Escherichia coli O78:H11 (strain H10407 / ETEC) SIMILARITY: Belongs to the glycosyltransferase 9 family. FUNCTION: Glycosylates adhesin TibA. Specifically adds anomer D-glycero-beta-D-manno-heptose. Can not use ADP-L-glycero-beta-D-manno-heptose as a sugar donnor. KEYWORDS: 3D-structure;Glycosyltransferase;Iron;Metal-binding;Transferase MISCELLANEOUS: Able to functionally replace the autotransporter adhesin heptosyltransferase (AAH) of the AIDA system in fully restoring activity to the AIDA-I adhesin depending on the modification of AIDA-I with heptose residues. Q9S5X0,"PROTEIN NAMES: K(+)-stimulated pyrophosphate-energized sodium pump (Membrane-bound sodium-translocating pyrophosphatase) (Pyrophosphate-energized inorganic pyrophosphatase) (Na(+)-PPase) (Tm-PPase) PROTEIN FAMILY: H(+)-translocating pyrophosphatase family, K(+)-stimulated subfamily ORGANISM: Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) SIMILARITY: Belongs to the H(+)-translocating pyrophosphatase (TC 3.A.10) family. K(+)-stimulated subfamily. FUNCTION: Sodium pump that utilizes the energy of pyrophosphate hydrolysis as the driving force for Na(+) movement across the membrane. KEYWORDS: 3D-structure;Calcium;Cell inner membrane;Cell membrane;Ion transport;Magnesium;Membrane;Metal-binding;Potassium;Reference proteome;Sodium;Sodium transport;Translocase;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell inner membrane ; Multi-pass membrane protein " Q9S745,"PROTEIN NAMES: Lipase-like PAD4 (Protein ENHANCED DISEASE SUSCEPTIBILITY 9) (Protein PHYTOALEXIN DEFICIENT 4) (AtPAD4) PROTEIN FAMILY: AB hydrolase superfamily, Lipase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the AB hydrolase superfamily. Lipase family. FUNCTION: Probable lipase required downstream of MPK4 for accumulation of the plant defense-potentiating molecule, salicylic acid, thus contributing to the plant innate immunity against invasive biotrophic pathogens and to defense mechanisms upon recognition of microbe-associated molecular patterns (MAMPs). Participates in the regulation of various molecular and physiological processes that influence fitness. Together with SG101, required for programmed cell death (PCD) triggered by NBS-LRR resistance proteins (e.g. RPS4, RPW8.1 and RPW8.2) in response to the fungal toxin fumonisin B1 (FB1) and avirulent pathogens (e.g. P.syringae pv. tomato strain DC3000 avrRps4 and pv. maculicola, turnip crinkle virus (TCV), and H.arabidopsidis isolates CALA2, EMOY2, EMWA1 and HIND4). Together with EDS1, confers a basal resistance by restricting the growth of virulent pathogens (e.g. H.arabidopsidis isolates NOCO2 and EMCO5, E.orontii isolate MGH, and P.syringae pv. tomato strain DC3000 or expressing HopW1-1 (HopPmaA)). Necessary for the salicylic acid-(SA-) dependent systemic acquired resistance (SAR) response that involves expression of multiple defense responses, including synthesis of the phytoalexin camalexin and expression of pathogenesis-related genes (e.g. PR1, ALD1, BGL2 and PR5) in response to pathogens, triggering a signal amplification loop that increases SA levels via EDS5 and SID2, but, together with EDS1, seems to repress the ethylene/jasmonic acid (ET/JA) defense pathway. May also function in response to abiotic stresses such as UV-C light and LSD1-dependent acclimatization to light conditions that promote excess excitation energy (EEE), probably by transducing redox signals and modulating stomatal conductance. Regulates the formation of lysigenous aerenchyma in hypocotyls in response to hypoxia, maybe via hydrogen peroxide production. Modulates leaf senescence in insect-infested tissue and triggers a phloem-based defense mechanism including antibiosis (e.g. green peach aphid (GPA), M.persicae) to limit phloem sap uptake and insect growth, thus providing an EDS1-independent basal resistance to insects. Also involved in regulation of root meristematic zone-targeted growth arrest together with EDS1 and in a VICTR-dependent manner. KEYWORDS: 3D-structure;Cytoplasm;Ethylene signaling pathway;Hydrolase;Hypersensitive response;Jasmonic acid signaling pathway;Lipid degradation;Lipid metabolism;Nucleus;Plant defense;Reference proteome;Transferase SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=Can move to the cytoplasm when in complex with EDS1. " Q9S7B5,"PROTEIN NAMES: Threonine synthase 1, chloroplastic (Protein METHIONINE OVER-ACCUMULATOR 2) PROTEIN FAMILY: Threonine synthase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the threonine synthase family. FUNCTION: Catalyzes the gamma-elimination of phosphate from L-phosphohomoserine and the beta-addition of water to produce L-threonine. PATHWAY: Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 5/5. KEYWORDS: 3D-structure;Allosteric enzyme;Amino-acid biosynthesis;Chloroplast;Lyase;Plastid;Pyridoxal phosphate;Reference proteome;S-adenosyl-L-methionine;Threonine biosynthesis;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast. MISCELLANEOUS: Binds 4 S-adenosyl-L-methionine (SAM) molecules per dimer. Although SAM3 and SAM4 have equivalent positions, their interactions with the protein are not identical. SAM3 interacts with Lys-181 and Asn-187 of monomer B, whereas SAM4 interacts only with Lys-181 of monomer A.; MISCELLANEOUS: Much more active than TS2 at physiological concentrations of S-adenosyl-L-methionine (20 uM)." Q9S7D1,"PROTEIN NAMES: Digalactosyldiacylglycerol synthase 1, chloroplastic PROTEIN FAMILY: Glycosyltransferase group 1 family, Glycosyltransferase 4 subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 4 subfamily. FUNCTION: Involved in the synthesis of diacylglycerol galactolipids that are specifically found in thylakoid membranes. Specific for alpha-glycosidic linkages. Responsible for the final assembly of galactolipids in photosynthetic membranes. Digalactosyldiacylglycerol (DGDG) provides stability to the photosystem I (PSI) complex, especially to the PsaA, PsaB, PsaC, PsaL and PsaH subunits. KEYWORDS: Alternative splicing;Chloroplast;Glycosyltransferase;Membrane;Plastid;Plastid outer membrane;Reference proteome;Transferase;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast outer membrane " Q9S7L2,PROTEIN NAMES: Transcription factor MYB98 (Myb-related protein 98) (AtMYB98) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Transcription factor that binds to the motif 5'-GTAACNT-3' in the promoter of target genes (e.g. DD11 and DD18) and promotes their expression within synergid cells (e.g. in the filiform apparatus) in ovules. Required for the formation of the filiform apparatus during synergid cell differentiation in the female gametophyte. Involved in pollen tube guidance to the micropyle. KEYWORDS: DNA-binding;Nucleus;Reference proteome;Repeat;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus Note=Localized to the nuclei of the synergid cells. Q9S834,"PROTEIN NAMES: ATP-dependent Clp protease proteolytic subunit 5, chloroplastic (Endopeptidase ClpP5) (nClpP5) (nClpP1) PROTEIN FAMILY: Peptidase S14 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the peptidase S14 family. FUNCTION: Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins (By similarity). KEYWORDS: Acetylation;Chloroplast;Direct protein sequencing;Hydrolase;Plastid;Protease;Reference proteome;Serine protease;Transit peptide;Ubl conjugation SUBCELLULAR LOCATION: Plastid, chloroplast stroma " Q9S850,PROTEIN NAMES: Sulfite oxidase (Moco-containing protein AtMCP) (At-SO) (AtSOX) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Probably involved in sulfite oxidative detoxification. PATHWAY: Energy metabolism; sulfur metabolism. KEYWORDS: 3D-structure;Alternative splicing;Metal-binding;Molybdenum;Oxidoreductase;Peroxisome;Reference proteome SUBCELLULAR LOCATION: Peroxisome Q9S9N9,"PROTEIN NAMES: Cinnamoyl-CoA reductase 1 (AtCCR1) (Protein IRREGULAR XYLEM 4) PROTEIN FAMILY: NAD(P)-dependent epimerase/dehydratase family, Dihydroflavonol-4-reductase subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the NAD(P)-dependent epimerase/dehydratase family. Dihydroflavonol-4-reductase subfamily. FUNCTION: Involved in the latter stages of lignin biosynthesis. Catalyzes one of the last steps of monolignol biosynthesis, the conversion of cinnamoyl-CoAs into their corresponding cinnamaldehydes. PATHWAY: Aromatic compound metabolism; phenylpropanoid biosynthesis. KEYWORDS: Alternative splicing;Disulfide bond;Lignin biosynthesis;NADP;Oxidoreductase;Phosphoprotein;Reference proteome " Q9SA41,PROTEIN NAMES: Metacaspase-8 (AtMC8) (Metacaspase 2e) (AtMCP2e) PROTEIN FAMILY: Peptidase C14B family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the peptidase C14B family. FUNCTION: Cysteine protease that cleaves specifically after arginine residues. Does not cleave caspase-specific substrates. May be involved in the modulation of programmed cell death activated by oxidative stress. KEYWORDS: Autocatalytic cleavage;Hydrolase;Protease;Reference proteome;S-nitrosylation;Thiol protease Q9SAJ6,"PROTEIN NAMES: Glyceraldehyde-3-phosphate dehydrogenase GAPCP1, chloroplastic (Glyceraldehyde-3-phosphate dehydrogenase of plastid 1) (NAD-dependent glyceraldehydephosphate dehydrogenase chloroplastic 1) PROTEIN FAMILY: Glyceraldehyde-3-phosphate dehydrogenase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family. FUNCTION: Involved in plastidial glycolytic pathway and plays a specific role in glycolytic energy production in non-green plastids and chloroplasts. Essential for breakdown of starch to form sucrose for export to non-photosynthetic tissues, and to generate primary metabolites for anabolic pathways such as fatty acid and amino acid synthesis. Plays an important role in plant development by providing substrates for the phosphorylated pathway of serine biosynthesis in roots. Plays a crucial role in pollen development. Functionally redundant with GAPCP2. KEYWORDS: Acetylation;Chloroplast;Glycolysis;NAD;Oxidoreductase;Plastid;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast stroma MISCELLANEOUS: Plants contain three types of GAPDH: NAD-dependent cytosolic forms which participate in glycolysis, NAD-dependent chloroplastic forms which participate in plastidic glycolysis and NADP-dependent chloroplastic forms which participate in the photosynthetic reductive pentose phosphate pathway (Calvin-Benson cycle). All the forms are encoded by distinct genes." Q9SB64,PROTEIN NAMES: Protein NBR1 homolog (AtNBR1) (At4g24690) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Autophagic substrate degraded in the vacuole by non-selective autophagy. Requires ATG8 protein expression to be recognized as an autophagic substrate. Acts probably as a receptor for autophagosomal degradation of ubiquitinated proteins. Targets ubiquitinated protein aggregates derived from denatured or damaged non-native proteins generated under stress conditions. Functions additively with the E3 ubiquitin-protein ligase CHIP for autophagosomal degradation of proteotoxic aggregates formed under stress conditions. KEYWORDS: 3D-structure;Acetylation;Autophagy;Cytoplasm;Metal-binding;Protein transport;Reference proteome;Stress response;Transport;Vacuole;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cytoplasm Vacuole Note=Forms large punctated cytoplasmic structures. Recruited to the central vacuole by non-selective autophagy. Q9SB81,"PROTEIN NAMES: Peroxidase 42 (Atperox P42) (ATP1a/ATP1b) (PRXR1) PROTEIN FAMILY: Peroxidase family, Classical plant (class III) peroxidase subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily. FUNCTION: Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.; FUNCTION: Might function as heat shock-like defense protein. KEYWORDS: Biological rhythms;Calcium;Disulfide bond;Glycoprotein;Heme;Hydrogen peroxide;Iron;Metal-binding;Oxidoreductase;Peroxidase;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted MISCELLANEOUS: There are 73 peroxidase genes in A.thaliana." Q9SB92,"PROTEIN NAMES: Protein WUSCHEL (AtWUS) (Plant growth activator 6) PROTEIN FAMILY: WUS homeobox family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the WUS homeobox family. FUNCTION: Transcription factor that plays a central role during early embryogenesis, oogenesis and flowering, probably by regulating expression of specific genes. Required to specify stem cell identity in meristems, such as shoot apical meristem (SAM). May induce shoot stem cells activity in order to maintain the stem cell identity. Involved in the developmental root meristem. In shoot apices, it is sufficient to induce the expression of CLV3, a putative ligand of the CLV signaling pathway. Also required to sustain organogenesis in the floral meristem by contributing to the expression of its own repressor, the AGAMOUS (AG) gene at the end of flower development. Binds directly to the 5'-TTAAT[GC][GC]-3' DNA sequence in the regulatory sequence of AG and activates its expression directly. Regulates one important step in ovule development to induce integument formation from the underlying chalazal domain. Participates in the promotion of vegetative to embryonic transition. Required to repress LEC1 expression. KEYWORDS: 3D-structure;Activator;Developmental protein;Differentiation;DNA-binding;Flowering;Homeobox;Nucleus;Oogenesis;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " Q9SBL1,"PROTEIN NAMES: Cyanohydrin beta-glucosyltransferase (UDP-glucose-p-hydroxymandelonitrile glucosyltransferase) PROTEIN FAMILY: UDP-glycosyltransferase family ORGANISM: Sorghum bicolor (Sorghum) (Sorghum vulgare) SIMILARITY: Belongs to the UDP-glycosyltransferase family. FUNCTION: Involved in the biosynthesis of the cyanogenic glucoside dhurrin. Prevents the dissociation and release of toxic hydrogen cyanide. Mandelonitrile, p-hydroxymandelonitrile, benzyl alcohol, benzoic acid and geraniol, but not hydroquinone(1,4-benzenediol), alpha-terpinol, linalool or farnesol are utilized as acceptor substrates. UDP-glucose, but not UDP-xylose or UDP-glucuronic acid can be used as sugar donor. PATHWAY: Secondary metabolite biosynthesis; dhurrin biosynthesis; dhurrin from L-tyrosine: step 3/3. KEYWORDS: 3D-structure;Cytoplasm;Direct protein sequencing;Endoplasmic reticulum;Glycosyltransferase;Membrane;Transferase SUBCELLULAR LOCATION: Cytoplasm Endoplasmic reticulum membrane ; Peripheral membrane protein ; Cytoplasmic side Note=In the presence of CYP79A1 and CYP71E1, moves toward the surface of the ER membranes in order to form a metabolon. " Q9SD71,"PROTEIN NAMES: E3 ubiquitin-protein ligase SGIP1 (SGS3-INTERACTING PROTEIN 1) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: E3 ubiquitin-protein ligase which triggers the ubiquitination and subsequent degradation of SGS3 in response to heat. Involved in the mechanisms necessary for quick response to heat and subsequent heritable transgenerational memory of heat acclimation (global warming) such as early flowering and attenuated immunity; this process includes epigenetic regulation as well as post-transcriptional gene silencing (PTGS). In response to heat, HSFA2 is activated and promotes the expression of REF6 which in turn derepresses HSFA2, thus establishing an heritable feedback loop able to trigger SGIP1 and subsequent SGIP1-mediated SGS3 degradation; this prevents the biosynthesis of trans-acting siRNA (tasiRNA) and leads to the release of HTT5, which drives early flowering but attenuates immunity. PATHWAY: Protein degradation; proteasomal ubiquitin-dependent pathway.; PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Reference proteome;Stress response;Transferase;Ubl conjugation pathway SUBCELLULAR LOCATION: Cytoplasmic granule Note=Co-localizes with SGS3 in cytoplasmic granules. " Q9SEE5,"PROTEIN NAMES: Galactokinase (Galactose kinase) PROTEIN FAMILY: GHMP kinase family, GalK subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the GHMP kinase family. GalK subfamily. FUNCTION: Sugar-1-kinase with a very high substrate specificity for the alpha-anomeric configuration of D-galacose (D-Gal). Converts also efficiently 2-deoxy-D-Gal to 2-deoxy-D-al-1-phosphate. PATHWAY: Carbohydrate metabolism; galactose metabolism. KEYWORDS: Acetylation;ATP-binding;Calcium;Carbohydrate metabolism;Galactose metabolism;Kinase;Magnesium;Manganese;Metal-binding;Nucleotide-binding;Reference proteome;Transferase " Q9SFV2,PROTEIN NAMES: FHA domain-containing protein FHA2 (Protein FORKHEAD-ASSOCIATED DOMAIN 2) (AtFHA2) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: May play a role in the control of plant organ development and specifically in the regulation of stamen development. Does not show transactivation activity in yeast. KEYWORDS: Acetylation;Developmental protein;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus Note=Detected at discrete loci in the nucleus. Q9SG92,"PROTEIN NAMES: Methylesterase 17 (AtMES17) (Methyl indole-3-acetic acid esterase) PROTEIN FAMILY: AB hydrolase superfamily, Methylesterase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the AB hydrolase superfamily. Methylesterase family. FUNCTION: Methylesterase that efficiently and specifically hydrolyzes methyl indole-3-acetic acid (MeIAA) to IAA (auxin). MeIAA is believed to be an inactive form of auxin that needs to be demethylated to exert a biological effect. PATHWAY: Plant hormone biosynthesis. KEYWORDS: Hydrolase;Reference proteome " Q9SGH4,"PROTEIN NAMES: Photosynthetic NDH subunit of lumenal location 3, chloroplastic (PsbQ-like protein 2) PROTEIN FAMILY: PsbQ family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the PsbQ family. FUNCTION: NDH shuttles electrons from NAD(P)H:plastoquinone, via FMN and iron-sulfur (Fe-S) centers, to quinones in the photosynthetic chain and possibly in a chloroplast respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton translocation, and thus conserves the redox energy in a proton gradient (Probable). Required for both formation and activity of the chloroplast NAD(P)H dehydrogenase (NDH) complex. KEYWORDS: 3D-structure;Chloroplast;Direct protein sequencing;Membrane;Plastid;Reference proteome;Thylakoid;Transit peptide;Transport SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane ; Peripheral membrane protein ; Lumenal side Note=Associated with the chloroplast NAD(P)H dehydrogenase/photosystem I (NDH/PSI) supercomplex. " Q9SGH6,"PROTEIN NAMES: Alpha-dioxygenase 1 (Alpha DOX1) (Fatty acid dioxygenase AlphaDOX1) (Pathogen-induced oxygenase) (Plant alpha dioxygenase 1) PROTEIN FAMILY: Peroxidase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the peroxidase family. FUNCTION: Alpha-dioxygenase that catalyzes the primary oxygenation step of a variety of 14-20 carbon fatty acids, containing up to three unsaturated bonds, into their corresponding 2R-hydroperoxides. Involved in the production of oxylipins that function in cell signaling, wound healing, and protection from infection. Mediates protection against oxidative stress and cell death, probably by generating some lipid-derived molecules. Promotes local and systemic plant defense in a salicylic acid (SA)-dependent manner, including the establishment of systemic acquired resistance (SAR) in response to incompatible interaction. Involved in a negative regulation of abscisic acid (ABA)-mediated signaling pathway. KEYWORDS: 3D-structure;Dioxygenase;Fatty acid biosynthesis;Fatty acid metabolism;Heme;Hypersensitive response;Iron;Lipid biosynthesis;Lipid droplet;Lipid metabolism;Metal-binding;Oxidoreductase;Oxylipin biosynthesis;Peroxidase;Plant defense;Reference proteome SUBCELLULAR LOCATION: Lipid droplet Note=Localizes on the surface of leaf oil bodies. " Q9SGU3,"PROTEIN NAMES: Transcription factor MYB72 (Myb-related protein 72) (AtMYB72) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Involved in metal ions homeostasis, including iron ions (Fe) acquisition, via the regulation of NAS4 and NAS2 genes expression. Necessary for plant survival in alkaline soil where iron availability is greatly restricted. Involved in the up-regulation of several biosynthesis genes of secondary metabolites involved in iron uptake under conditions of iron deficiency. Triggers tolerance to nickel (Ni) and zinc (Zn) ions. Required in the roots during early signaling steps of rhizobacteria-mediated (e.g. P.fluorescens WCS417r) and beneficial fungi-mediated (e.g. T.asperellum T34) broad-spectrum induced systemic resistance (ISR) against several pathogens (e.g. P.syringae pv tomato, H.parasitica, P.cucumerina, A.brassicicola and B.cinerea) and implying enhanced callose deposition. Required for the induction of some genes (e.g. BGLU42) upon rhizobacteria-mediated ISR. KEYWORDS: DNA-binding;Nucleus;Plant defense;Reference proteome;Repeat;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " Q9SGW3,PROTEIN NAMES: 26S proteasome non-ATPase regulatory subunit 8 homolog A (26S proteasome regulatory subunit RPN12a) (AtRPN12a) (26S proteasome regulatory subunit S14 homolog A) PROTEIN FAMILY: Proteasome subunit S14 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the proteasome subunit S14 family. FUNCTION: Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins. May help to control the degradation of one or more factors that repress cytokinin signaling. Plays an important role for balancing cell expansion with cell proliferation rates during shoot development. KEYWORDS: Acetylation;Cytokinin signaling pathway;Proteasome;Reference proteome;Ubl conjugation Q9SHG6,"PROTEIN NAMES: Septin and tuftelin-interacting protein 1 homolog 1 (Nineteen complex-related protein 1 homolog) (AtNTR1) (Protein SPLICEOSOMAL TIMEKEEPER LOCUS 1) PROTEIN FAMILY: TFP11/STIP family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the TFP11/STIP family. FUNCTION: Involved in pre-mRNA splicing, specifically in spliceosome disassembly during late-stage splicing events (By similarity). Involved in snRNPs recycling. Required for efficient splicing of genes that act within the plant circadian clock. Part of a transcription elongation checkpoint at alternative exons. Required for correct expression and splicing of DOG1, a regulator of seed dormancy. May induce transient transcriptional pausing of polymerase II at slices sites. KEYWORDS: Coiled coil;DNA-binding;mRNA processing;mRNA splicing;Nucleus;Reference proteome;Spliceosome SUBCELLULAR LOCATION: Nucleus Note=Excluded from the nucleolus. Co-localizes with polymerase II. MISCELLANEOUS: Physically located at the target splice sites." Q9SHL8,PROTEIN NAMES: Protein PIN-LIKES 5 (Auxin efflux carrier-like protein 5) PROTEIN FAMILY: Auxin efflux carrier family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the auxin efflux carrier (TC 2.A.69.2) family. FUNCTION: Involved in cellular auxin homeostasis by regulating auxin metabolism. Regulates intracellular auxin accumulation at the endoplasmic reticulum and thus auxin availability for nuclear auxin signaling. KEYWORDS: Auxin signaling pathway;Endoplasmic reticulum;Membrane;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein Q9SHU7,"PROTEIN NAMES: Protein TIC 21, chloroplastic (Protein CHLOROPLAST IMPORT APPARATUS 5) (AtCIA5) (Protein PERMEASE IN CHLOROPLASTS 1) (AtPIC1) (Translocon at the inner envelope membrane of chloroplasts 21) (AtTIC21) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Involved in chloroplast protein import across the inner envelope membrane. Acts also as a chloroplast permease regulating the iron transport and homeostasis. Involved in the uptake and sequestration of iron in plastids. KEYWORDS: Chloroplast;Direct protein sequencing;Membrane;Plastid;Plastid inner membrane;Protein transport;Reference proteome;Transit peptide;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Plastid, chloroplast inner membrane ; Multi-pass membrane protein " Q9SI37,"PROTEIN NAMES: WRKY transcription factor 1 (Transcription factor ZAP1) (WRKY DNA-binding protein 1) (Zinc-dependent activator protein 1) PROTEIN FAMILY: WRKY group I family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the WRKY group I family. FUNCTION: Transcription factor. Binds to a 5'-CGTTGACCGAG-3' consensus core sequence which contains a W box, a frequently occurring elicitor-responsive cis-acting element. KEYWORDS: 3D-structure;Activator;Alternative splicing;DNA-binding;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Repeat;Transcription;Transcription regulation;Zinc SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: Binding to target DNA is mediated mainly by the C-terminal WRKY domain, while part of the activation domain is located between positions 210 and 285." Q9SIK7,"PROTEIN NAMES: Presenilin-like protein At2g29900 PROTEIN FAMILY: Peptidase A22A family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the peptidase A22A family. FUNCTION: Probable subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors. KEYWORDS: Endoplasmic reticulum;Golgi apparatus;Membrane;Notch signaling pathway;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein Golgi apparatus membrane ; Multi-pass membrane protein " Q9SIS3,"PROTEIN NAMES: 65-kDa microtubule-associated protein 6 (AtMAP65-6) PROTEIN FAMILY: MAP65/ASE1 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the MAP65/ASE1 family. FUNCTION: Microtubule-associated protein that mediates the formation of a mesh-like stable and dense network formed by individual microtubules (MT). Confers MT resistance to high concentration of NaCl. KEYWORDS: Alternative splicing;Cell cycle;Cell division;Coiled coil;Cytoplasm;Cytoskeleton;Microtubule;Mitochondrion;Mitosis;Nucleus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Nucleus Cytoplasm. Mitochondrion. Cytoplasm, cytoskeleton, phragmoplast. Note=Associated with mitochondria. Binds to MT in preprophase band, during anaphase, and in phragmoplast. " Q9SIT1,"PROTEIN NAMES: Receptor-like kinase TMK3 (BARK1-like kinase 2) (Leucine-rich repeat receptor-like kinases TMK3) (Transmembrane kinase 3) PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. FUNCTION: Involved in auxin signal transduction and cell expansion and proliferation regulation. KEYWORDS: 3D-structure;ATP-binding;Disulfide bond;Glycoprotein;Kinase;Leucine-rich repeat;Membrane;Nucleotide-binding;Receptor;Reference proteome;Repeat;Serine/threonine-protein kinase;Signal;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Single-pass membrane protein " Q9SIV3,"PROTEIN NAMES: Transcription activator GLK1 (GBF'S PRO-RICH REGION-INTERACTING factor 1) (Golden2-like protein 1) (AtGLK1) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Transcriptional activator that functions with GLK2 to promote chloroplast development. Acts as an activator of nuclear photosynthetic genes involved in chlorophyll biosynthesis, light harvesting, and electron transport. Acts in a cell-autonomous manner to coordinate and maintain the photosynthetic apparatus within individual cells. May function in photosynthetic capacity optimization by integrating responses to variable environmental and endogenous cues. Prevents premature senescence. KEYWORDS: Activator;Alternative splicing;DNA-binding;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: Plants overexpressing GLK1 have a delay in flowering under long days, show high constitutive expression of genes encoding disease defense related proteins and are resistant to the pathogen F.graminearum." Q9SIW2,"PROTEIN NAMES: Protein CHROMATIN REMODELING 35 (Protein DEFECTIVE IN MERISTEM SILENCING 1) (Protein DEFECTIVE IN RNA-DIRECTED DNA METHYLATION 1) PROTEIN FAMILY: Helicase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the helicase family. FUNCTION: Subunit of the chromatin-remodeling complex (DDR complex) that mediates RNA polymerases IV and V (Pol IV and Pol V) recruitment to chromatin. Cooperates with Pol IV and Pol V to regulates RNA- and RNAi- (RNA interference) directed non-CpG de novo DNA methylation on cytosine of genes targeted for silencing and enhancers, also known as siRNA-directed DNA methylation (RdDM), thus leading to epigenetic modification of the genome and promoting/maintaining heterochromatin. In collaboration with Pol V, mediates/maintains, in cis, methylation-associated self-silencing of exogenous transgene transcribing inverted-repeat (exo-IR) silencer (exo-Pdsi) to restrain exo-IR dsRNA accumulation and subsequent inappropriate silencing of active protein-coding genes (e.g. PDS) by exo-IR-derivating 24-nt siRNAs. Also required to mediate loss of CpG methylation when the silencing inducer is withdrawn. Required for the maintenance of retrotransposon large terminal repeats (LTRs) and transposable elements (TE) edges silencing mediated by cytosine methylation. Required for transcriptional repression of specific classes of pericentromeric 180-bp repeats by promoting condensation and histone H3 lysine 9 dimethylation (H3K9me2) at chromocenters. Required for basal resistance against the necrotrophic fungal pathogen Plectosphaerella cucumerina. KEYWORDS: 3D-structure;ATP-binding;DNA-binding;Helicase;Hydrolase;Nucleotide-binding;Nucleus;Plant defense;Reference proteome;RNA-mediated gene silencing SUBCELLULAR LOCATION: Nucleus Note=Excluded from the nucleolus. Colocalizes with endogenous repeat loci. " Q9SIY3,"PROTEIN NAMES: Triphosphate tunnel metalloenzyme 3 (Adenosinetriphosphatase) (ATPase) (Triphosphatase) (PPPase) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Involved in the hydrolysis of the beta-gamma-phosphoanhydride linkage of triphosphate-containing substrates (inorganic or nucleoside-linked). Catalyzes the hydrolysis of inorganic triphosphate (PPPi), however it does not display significant activity towards long-chain polyphosphates. The existence of PPPi in living cells is still unclear, and PPPase activity might be the ancestral function of CYTH domain. It also has gamma-phosphatase activity on NTP substrates, but no adenylate cyclase or RNA triphosphatase activity. KEYWORDS: 3D-structure;Alternative splicing;ATP-binding;Hydrolase;Nucleotide-binding;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus " Q9SJ05,"PROTEIN NAMES: Shikimate kinase 1, chloroplastic (AtSK1) PROTEIN FAMILY: Shikimate kinase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the shikimate kinase family. FUNCTION: Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate. PATHWAY: Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 5/7. KEYWORDS: Alternative splicing;Amino-acid biosynthesis;Aromatic amino acid biosynthesis;ATP-binding;Chloroplast;Disulfide bond;Kinase;Magnesium;Metal-binding;Nucleotide-binding;Plastid;Reference proteome;Stress response;Transferase;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast MISCELLANEOUS: SK1 forms a homodimer in solution, which may facilitate its relative thermostability when exposed at 37 degrees Celsius." Q9SJJ3,PROTEIN NAMES: BEL1-like homeodomain protein 8 (BEL1-like protein 8) (Protein POUND-FOOLISH) PROTEIN FAMILY: TALE/BELL homeobox family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the TALE/BELL homeobox family. FUNCTION: Required for specifying floral primordia and establishing early internode patterning events during inflorescence development. KEYWORDS: DNA-binding;Homeobox;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus Q9SJR5,"PROTEIN NAMES: Growth-regulating factor 3 (AtGRF3) (Transcription activator GRF3) PROTEIN FAMILY: GRF family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the GRF family. FUNCTION: Transcription activator that plays a role in the regulation of cell expansion in leaf and cotyledons tissues. Component of a network formed by miR396, the GRFs and their interacting factors (GIFs) acting in the regulation of meristem function, at least partially through the control of cell proliferation. microRNA396-GRF1/GRF3 regulatory module acts as a developmental regulator in the reprogramming of root cells during cyst nematode infection, leading to the formation of the syncytium. KEYWORDS: Activator;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " Q9SJT1,PROTEIN NAMES: SUMO-activating enzyme subunit 2 (Protein EMBRYO DEFECTIVE 2764) (Ubiquitin-like 1-activating enzyme E1B) PROTEIN FAMILY: Ubiquitin-activating E1 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the ubiquitin-activating E1 family. FUNCTION: The dimeric enzyme acts as an E1 ligase for SUMO1 and SUMO2. It mediates ATP-dependent activation of SUMO proteins and formation of a thioester with a conserved cysteine residue on SAE2. PATHWAY: Protein modification; protein sumoylation. KEYWORDS: 3D-structure;Acetylation;Alternative splicing;ATP-binding;Metal-binding;Nucleotide-binding;Nucleus;Phosphoprotein;Reference proteome;Transferase;Ubl conjugation pathway;Zinc SUBCELLULAR LOCATION: Nucleus. Q9SJZ2,"PROTEIN NAMES: Peroxidase 17 (Atperox P17) (ATP25a) PROTEIN FAMILY: Peroxidase family, Classical plant (class III) peroxidase subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily. FUNCTION: Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue. KEYWORDS: Calcium;Disulfide bond;Glycoprotein;Heme;Hydrogen peroxide;Iron;Metal-binding;Oxidoreductase;Peroxidase;Reference proteome;Secreted;Signal;Vacuole SUBCELLULAR LOCATION: Secreted Vacuole Note=Carboxy-terminal extension appears to target the protein to vacuoles. MISCELLANEOUS: There are 73 peroxidase genes in A.thaliana." Q9SKB3,PROTEIN NAMES: Poly(ADP-ribose) glycohydrolase 1 PROTEIN FAMILY: Poly(ADP-ribose) glycohydrolase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the poly(ADP-ribose) glycohydrolase family. FUNCTION: Poly(ADP-ribose) synthesized after DNA damage is only present transiently and is rapidly degraded by poly(ADP-ribose) glycohydrolase (By similarity). Involved in establishing period length of the circadian oscillator. May regulate post-translational poly(ADP-ribosyl)ation of an oscillator component. KEYWORDS: Alternative splicing;Biological rhythms;Hydrolase;Reference proteome Q9SKD9,"PROTEIN NAMES: Probable WRKY transcription factor 46 (WRKY DNA-binding protein 46) PROTEIN FAMILY: WRKY group III family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the WRKY group III family. FUNCTION: Transcription factor involved in the regulation of osmotic stress responses and stomatal movement. Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element (By similarity). Positive regulator of EDS1-dependent defense against E.amylovora. Together with WRKY70 and WRKY53, promotes resistance to P.syringae, probably by enhancing salicylic acid (SA)- dependent genes. Contributes to the suppression of jasmonic acid (MeJA)-induced expression of PDF1.2. Together with WRKY54 and WRKY70, promotes brassinosteroid (BR)-regulated plant growth but prevent drought response by modulating gene expression. KEYWORDS: Brassinosteroid signaling pathway;DNA-binding;Jasmonic acid signaling pathway;Nucleus;Plant defense;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " Q9SKT0,"PROTEIN NAMES: Protein THYLAKOID FORMATION 1, chloroplastic PROTEIN FAMILY: THF1 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the THF1 family. FUNCTION: Involved in a dynamic process of vesicle-mediated thylakoid membrane biogenesis. Required for the normal organization of vesicles into mature thylakoid stacks and ultimately for leaf development. Also involved in a sugar-signaling mechanism in roots by mediating signaling between the plasma membrane and the plastid. Probably acts downstream of the plasma membrane-delimited heterotrimeric G-protein GPA1 in a D-glucose signaling pathway. KEYWORDS: Chloroplast;Coiled coil;Membrane;Plastid;Plastid outer membrane;Reference proteome;Transit peptide;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Plastid, chloroplast outer membrane; Single-pass membrane protein. Plastid, chloroplast stroma. " Q9SMN1,"PROTEIN NAMES: Gamma carbonic anhydrase-like 2, mitochondrial (AtCAL2) (GAMMA CAL2) PROTEIN FAMILY: Gamma-class carbonic anhydrase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the gamma-class carbonic anhydrase family. FUNCTION: Involved in complex I assembly in mitochondria and respiration. KEYWORDS: 3D-structure;Direct protein sequencing;Membrane;Metal-binding;Mitochondrion;Reference proteome;Transit peptide;Zinc SUBCELLULAR LOCATION: Mitochondrion membrane ; Peripheral membrane protein ; Matrix side Note=Probably integral to the membrane. " Q9SN38,"PROTEIN NAMES: Receptor-like protein 51 (AtRLP51) (Protein PUTATIVE DEVELOPMENTAL ORTHOLOG 1) (AtPDO1) (Protein SUPPRESSOR OF NPR1-1, CONSTITUTIVE 2) PROTEIN FAMILY: RLP family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the RLP family. FUNCTION: Involved in plant defense. Required for basal resistance against P.syringae pv. tomato DC3000. KEYWORDS: Cell membrane;Glycoprotein;Leucine-rich repeat;Membrane;Plant defense;Receptor;Reference proteome;Repeat;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein " Q9SN86,"PROTEIN NAMES: Malate dehydrogenase, chloroplastic (Chloroplastic malate dehydrogenase) (Chloroplastic MDH) (cpNAD-MDH) (Plastidic NAD-dependent malate dehydrogenase) (pNAD-MDH) PROTEIN FAMILY: LDH/MDH superfamily, MDH type 1 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the LDH/MDH superfamily. MDH type 1 family. FUNCTION: Catalyzes a reversible NAD-dependent dehydrogenase reaction involved in central metabolism and redox homeostasis between organelle compartments (Probable). Plays a key role in the metabolism of dark chloroplasts and non-green plastids. Essential for embryo viability. Plays an essential role in heterotrophic metabolism in embryos, and autotrophic metabolism in photosynthetic tissues as well. KEYWORDS: 3D-structure;Chloroplast;NAD;Oxidoreductase;Plastid;Reference proteome;Transit peptide;Tricarboxylic acid cycle SUBCELLULAR LOCATION: Plastid, chloroplast stroma " Q9SNB4,"PROTEIN NAMES: Transcription factor LUX (Protein LUX ARRHYTHMO) (Protein PHYTOCLOCK 1) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Transcription factor that is essential for the generation of the circadian clock oscillation. Is necessary for activation of CCA1 and LHY expression. Is coregulated with TOC1 and seems to be repressed by CCA1 and LHY by direct binding of these proteins to the evening element in the LUX promoter. Directly regulates the expression of PRR9, a major component of the morning transcriptional feedback circuit, by binding specific sites on PRR9 promoter. Binds to its own promoter, inducing a negative auto-regulatory feedback loop within the core clock. Binds to ELF3 and associates with ELF4 in a diurnal complex which is required for the expression of the growth-promoting transcription factors PIF4 and PIF5 and subsequent hypocotyl growth in the early evening. KEYWORDS: 3D-structure;Alternative splicing;Biological rhythms;DNA-binding;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " Q9SNB7,"PROTEIN NAMES: Protein LOW PHOTOSYNTHETIC EFFICIENCY 1, chloroplastic PROTEIN FAMILY: PPR family, P subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the PPR family. P subfamily. FUNCTION: Required for light-regulated photosystem II (PSII) biogenesis and grana thylakoids formation by binding to the 5' UTR of PSII subunit mRNAs (e.g. psbJ, psbN and psbA) in a light-dependent manner through a redox-based mechanism, and facilitating the association of HCF173 with target mRNAs, which encodes PSII reaction center proteins (e.g. J, N and D1), thus regulating its expression by modulating ribosome loading. KEYWORDS: Chloroplast;Membrane;Plastid;Reference proteome;Repeat;RNA-binding;Thylakoid;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane ; Peripheral membrane protein ; Stromal side Plastid, chloroplast stroma Note=Predominantly present at thylakoid membranes. " Q9SPG2,"PROTEIN NAMES: Transcription factor MYB28 (Myb-related protein 28) (AtMYB28) (Protein HIGH ALIPHATIC GLUCOSINOLATE 1) (Protein PRODUCTION OF METHIONINE-DERIVED GLUCOSINOLATE 1) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Major regulator of short-chained aliphatic glucosinolates (GLSs) biosynthesis. Together with MYB29/HAG3 and MYB76/HAG2, promotes aliphatic glucosinolate biosynthesis but represses indolic glucosinolate biosynthesis. Prevents insect performance (e.g. lepidopteran insect Mamestra brassicae and Spodoptera exigua) by promoting glucosinolates. KEYWORDS: Alternative splicing;DNA-binding;Nucleus;Reference proteome;Repeat;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " Q9SPK4,"PROTEIN NAMES: Phosphoenolpyruvate carboxylase kinase 1 (AtPPCK1) PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. FUNCTION: Calcium-independent kinase involved in light-dependent phosphoenolpyruvate carboxylase phosphorylation. KEYWORDS: Alternative splicing;ATP-binding;Kinase;Nucleotide-binding;Pyruvate;Reference proteome;Serine/threonine-protein kinase;Transferase MISCELLANEOUS: Lacks the autoinhibitory region and EF hands found in calcium-dependent protein kinases." Q9SQH6,"PROTEIN NAMES: Auxin efflux carrier component 6 (AtPIN6) PROTEIN FAMILY: Auxin efflux carrier family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the auxin efflux carrier (TC 2.A.69.1) family. FUNCTION: Component of the intracellular auxin-transport pathway. Regulates auxin transport and auxin homeostasis. Directly involved in the regulation of nectar production. Involved in unfolded protein response (UPR) activation. Involved in the control of vein patterning. Redundantly with PIN8, inhibits the vein-formation-promoting functions of PIN5. PIN5, PIN6, and PIN8 control vein network geometry, but they are expressed in mutually exclusive domains of leaf vascular cells. KEYWORDS: Auxin signaling pathway;Endoplasmic reticulum;Glycoprotein;Membrane;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein " Q9SQT8,"PROTEIN NAMES: Bifunctional 3-dehydroquinate dehydratase/shikimate dehydrogenase, chloroplastic (DHQ-SDH protein) (DHQase-SORase) (Protein EMBRYO DEFECTIVE 3004) [Includes: Dehydroquinate dehydratase (DHQ) ; Shikimate dehydrogenase (SDH) ] PROTEIN FAMILY: Type-I 3-dehydroquinase family; Shikimate dehydrogenase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: In the N-terminal section; belongs to the type-I 3-dehydroquinase family.; SIMILARITY: In the C-terminal section; belongs to the shikimate dehydrogenase family. FUNCTION: Bifunctional dehydroquinate dehydratase-shikimate dehydrogenase enzyme that catalyzes two steps in the chorismate biosynthesis pathway. PATHWAY: Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 3/7.; PATHWAY: Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 4/7. KEYWORDS: 3D-structure;Amino-acid biosynthesis;Aromatic amino acid biosynthesis;Chloroplast;Lyase;Multifunctional enzyme;NADP;Oxidoreductase;Plastid;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast " Q9SQY0,"PROTEIN NAMES: NAC domain containing protein 52 (ANAC052) (NAC domain containing protein 51) (ANAC051) (Protein POLLEN DEVELOPMENT DEFECTIVE 1) (Protein SUPPRESSOR OF GENE SILENCING 1) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Transcriptional repressor that binds to the motif 5'-(C/T)A(C/A)G-3' in the promoter of target genes. Binds also to the 5'-CTTGNNNNNCAAG-3' consensus sequence in chromatin. Can bind to the mitochondrial dysfunction motif (MDM) present in the upstream regions of mitochondrial dysfunction stimulon (MDS) genes involved in mitochondrial retrograde regulation (MRR). Together with NAC050 and JMJ14, regulates gene expression and flowering time by associating with the histone demethylase JMJ14, probably by the promotion of RNA-mediated gene silencing. Regulates siRNA-dependent post-transcriptional gene silencing (PTGS) through SGS3 expression modulation. Required during pollen development. KEYWORDS: Alternative splicing;Coiled coil;DNA-binding;Flowering;Nucleus;Plant defense;Reference proteome;Repressor;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " Q9SRK5,"PROTEIN NAMES: Phosphoglucan phosphatase LSF2, chloroplastic (Phosphoglucan phosphatase like sex Four2) (Protein LIKE SEX4 2) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Starch-associated phosphoglucan phosphatase that selectively dephosphorylates the glucan C3 position. Probably participates in the regulation of starch degradation. KEYWORDS: 3D-structure;Carbohydrate metabolism;Chloroplast;Hydrolase;Plastid;Protein phosphatase;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast " Q9SRX9,"PROTEIN NAMES: E3 ubiquitin-protein ligase NLA (Protein BENZOIC ACID HYPERSENSITIVE 1) (Protein NITROGEN LIMITATION ADAPTATION) (RING-type E3 ubiquitin transferase BAH1) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: E3 ubiquitin-protein ligase that mediates E2-dependent protein ubiquitination. Plays a role in salicylic acid-mediated negative feedback regulation of salicylic acid (SA) accumulation. May be involved in the overall regulation of SA, benzoic acid and phenylpropanoid biosynthesis. Involved in defense response. May act as negative regulator of resistance to the necrotrophic fungal pathogen Plectosphaerella cucumerina by modulating the accumulation of the phytoalexin camalexin and the salicylic acid- and jasmonate- dependent defense pathways. Controls the adaptability to nitrogen limitation by channeling the phenylpropanoid metabolic flux to the induced anthocyanin synthesis. Involved in the regulation of inorganic phosphate (Pi) homeostasis in a nitrate-dependent fashion. Directs the ubiquitination and subsequent degradation of the plasma membrane-localized inorganic phosphate transporters PHT1-1 and PHT1-4, to maintain phosphate homeostasis. The ubiquitination of PHTs triggers their clathrin-dependent endocytosis and trafficking to the vacuole through the endosomal pathway for degradation. Functions cooperatively with UBC24/PHO2 to regulate the abundance of PHT1-1, PHT1-2 and PHT1-3 in different subcellular compartments. Regulates Pi homeostasis by mediating, cooperatively with UBC24/PHO2, polyubiquitination of PHT1-4 and its targeting for degradation. Directs the polyubiquitination and subsequent degradation of the plasma membrane-localized nitrate transporter NPF2.13/NRT1.7, to help plants to adapt to nitrogen deficiency by regulating the source-to-sink remobilization of nitrate. Regulates leaf senescence during nitrogen deficiency by mediating, cooperatively with UBC24/PHO2, polyubiquitination of NAC92/ORE1 and its targeting for degradation. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Alternative splicing;Cell membrane;Membrane;Metal-binding;Nucleus;Reference proteome;Transferase;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus speckle Nucleus Cell membrane Note=Localizes at the plasma membrane, where it interacts with PHT1-1 and PHT1-4. Localizes at the plasma membrane, where it interacts with NPF2.13/NRT1.7. Localizes in the nucleus, where it interacts with NAC92/ORE1. MISCELLANEOUS: Is the target of miR827, a Pi starvation-induced microRNA." Q9SS43,PROTEIN NAMES: Xylan glycosyltransferase MUCI21 (Protein MUCILAGE-MODIFIED 5) (Protein MUCILAGE-RELATED 21) (Putative xylan xylosyltransgerase MUCI21) PROTEIN FAMILY: Glycosyltransferase 61 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the glycosyltransferase 61 family. FUNCTION: Glycosyletransferase required for the proper composition and structural properties of released seed coat mucilage. Required for the production of highly branched xylan polymers in seed coat mucilage. Facilitates the addition of xylose residues directly to the xylan backbone. Xylan with xylose side chains seems to be necessary for pectin attachment to the seed surface. Essential for xylan synthesis in seed coat epidermal (SCE) cells. KEYWORDS: Cell wall biogenesis/degradation;Glycoprotein;Glycosyltransferase;Golgi apparatus;Membrane;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus membrane ; Single-pass type II membrane protein Q9SS80,"PROTEIN NAMES: Protein OCTOPUS PROTEIN FAMILY: OCTOPUS family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the OCTOPUS family. FUNCTION: Potentiates primary root protophloem differentiation. Required, together with VCC, for embryo provasculature development and cotyledon vascular complexity and connectivity. Regulates roots architecture. Mediates the recruitment of ASK7/BIN2 to the plasma membrane. KEYWORDS: Cell membrane;Coiled coil;Cytoplasm;Developmental protein;Differentiation;Membrane;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cell membrane ; Peripheral membrane protein ; Cytoplasmic side Cytoplasm Note=Polar localization at the apical side of the plasma membrane, shootward oriented, in developing root protophloem cells. Also found in the cytoplasm in the early, dividing. protophloem cells. " Q9ST48,"PROTEIN NAMES: DELLA protein DWARF8 (Protein dwarf-8) PROTEIN FAMILY: GRAS family, DELLA subfamily ORGANISM: Zea mays (Maize) SIMILARITY: Belongs to the GRAS family. DELLA subfamily. FUNCTION: Probable transcriptional regulator that acts as a repressor of the gibberellin (GA) signaling pathway. Probably acts by participating in large multiprotein complexes that repress transcription of GA-inducible genes. Upon GA application, it is degraded by the proteasome, allowing the GA signaling pathway. KEYWORDS: Gibberellin signaling pathway;Nucleus;Phosphoprotein;Reference proteome;Repressor;Transcription;Transcription regulation;Ubl conjugation SUBCELLULAR LOCATION: Nucleus " Q9STF2,"PROTEIN NAMES: Protein PLASTID TRANSCRIPTIONALLY ACTIVE 16, chloroplastic (pTAC16) PROTEIN FAMILY: NAD(P)-dependent epimerase/dehydratase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the NAD(P)-dependent epimerase/dehydratase family. FUNCTION: Probably involved in the regulation of plastid gene expression. KEYWORDS: Alternative splicing;Chloroplast;Coiled coil;Membrane;NADP;Phosphoprotein;Plastid;Reference proteome;Thylakoid;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast stroma, chloroplast nucleoid Plastid, chloroplast thylakoid membrane ; Peripheral membrane protein ; Stromal side " Q9STN8,"PROTEIN NAMES: E3 ubiquitin-protein ligase SINAT4 (RING-type E3 ubiquitin transferase SINAT4) (Seven in absentia homolog 4) PROTEIN FAMILY: SINA (Seven in absentia) family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the SINA (Seven in absentia) family. FUNCTION: E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. It probably triggers the ubiquitin-mediated degradation of different substrates. Modulates directly the ubiquitination and proteasomal-dependent degradation of FREE1, a component of the ESCRT-I complex. Modulates directly the ubiquitination and proteasomal-dependent degradation of ELC/VPS23A, a component of the ESCRT-I complex. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Cytoplasmic vesicle;Endosome;Metal-binding;Reference proteome;Transferase;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Endosome, multivesicular body Cytoplasmic vesicle, autophagosome " Q9STP8,"PROTEIN NAMES: Acyl-CoA-binding domain-containing protein 2 (Acyl-CoA binding protein 2) PROTEIN FAMILY: ACBP family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the ACBP family. FUNCTION: Binds medium- and long-chain acyl-CoA esters with very high affinity. Can interact in vitro with palmitoyl-CoA, but not with oleoyl-CoA. Binds to lead ions (Pb). May function as an intracellular carrier of acyl-CoA esters. Required for proper phospholipid and, to a lower extent, galactolipid composition. KEYWORDS: ANK repeat;Cell membrane;Endoplasmic reticulum;Lead;Lipid-binding;Membrane;Metal-binding;Peroxisome;Reference proteome;Repeat;Signal-anchor;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein. Peroxisome membrane; Single-pass membrane protein. " Q9STY0,"PROTEIN NAMES: Peroxisomal membrane protein 11B (Peroxin-11B) (AtPEX11b) PROTEIN FAMILY: Peroxin-11 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the peroxin-11 family. FUNCTION: Involved in peroxisomal proliferation. Promotes peroxisomal duplication, aggregation or elongation without fission. KEYWORDS: Membrane;Peroxisome;Peroxisome biogenesis;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Peroxisome membrane ; Multi-pass membrane protein " Q9SW18,"PROTEIN NAMES: Magnesium protoporphyrin IX methyltransferase, chloroplastic PROTEIN FAMILY: Class I-like SAM-binding methyltransferase superfamily, Magnesium protoporphyrin O-methyltransferase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase superfamily. Magnesium protoporphyrin O-methyltransferase family. FUNCTION: Converts Mg-protoporphyrin IX to Mg-protoporphyrin IX methylester using S-adenosyl-L-methionine as a cofactor. Involved in chloroplast-to-nucleus signaling by acting as a negative effector of nuclear photosynthetic gene expression. PATHWAY: Porphyrin-containing compound metabolism; chlorophyll biosynthesis. KEYWORDS: Alternative splicing;Chlorophyll biosynthesis;Chloroplast;Membrane;Methyltransferase;Plastid;Reference proteome;S-adenosyl-L-methionine;Thylakoid;Transferase;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast membrane ; Peripheral membrane protein Plastid, chloroplast thylakoid membrane ; Peripheral membrane protein " Q9SWA6,"PROTEIN NAMES: Negative regulator of systemic acquired resistance SNI1 (Non-structural maintenance of chromosome element 5) (Non-SMC element 5) (Protein SUPPRESSOR OF NPR1, INDUCIBLE 1) (Suppressor of npr1-1) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Component of the SMC5-SMC6 complex, a complex involved in repair of DNA double-strand breaks by homologous recombination. Transcription repressor that prevents expression of pathogenesis-related genes (PR) via histone modifications and binding negative cis-acting elements at their promoters. Negative regulator of hypersensitive response (HR) and systemic acquired resistance (SAR) required to dampen the basal expression of pathogenesis related (PR) genes. Functions synergistically with NTL9/CBNAC as negative regulator of pathogen-induced PR1 expression and basal resistance to a virulent strain of P.syringae. Binds to the PR1 gene promoter to suppress defense response in the absence of pathogen challenge and is removed in response to induction. Negatively regulates both gene expression and DNA recombination during pathogen infection, thus being involved in short-term defense response and a long-term survival strategy. Prevents effective immune responses that involve activation of DNA damage responses, probably by negatively regulating the DNA damage sensors RAD17 and ATR. Negative regulator of defenses against the beet cyst nematode H.schachtii. KEYWORDS: DNA damage;DNA recombination;DNA repair;DNA-binding;Hypersensitive response;Nucleus;Plant defense;Reference proteome;Repressor;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus Note=Also detectable in some fluorescent loci peripheral to the nucleus. " Q9SWG3,PROTEIN NAMES: Protein FAR-RED IMPAIRED RESPONSE 1 PROTEIN FAMILY: FHY3/FAR1 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the FHY3/FAR1 family. FUNCTION: Transcription activator that recognizes and binds to the DNA consensus sequence 5'-CACGCGC-3'. Activates the expression of FHY1 and FHL involved in light responses. Positive regulator of chlorophyll biosynthesis via the activation of HEMB1 gene expression. KEYWORDS: Metal-binding;Nucleus;Reference proteome;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Q9SX85,"PROTEIN NAMES: Exocyst complex component SEC3A (AtSec3a) PROTEIN FAMILY: SEC3 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the SEC3 family. FUNCTION: Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane during regulated or polarized secretion. Involved in polarized cell growth and organ morphogenesis. During cytokinesis, involved in cell plate initiation, cell plate maturation and formation of new primary cell wall. During cytokinesis, involved in cell plate initiation, cell plate maturation and formation of new primary cell wall. KEYWORDS: Alternative splicing;Cell membrane;Coiled coil;Cytoplasm;Cytoskeleton;Exocytosis;Membrane;Reference proteome;Secreted;Transport SUBCELLULAR LOCATION: Cytoplasm, cytosol Cell membrane ; Peripheral membrane protein Cytoplasm, cytoskeleton, phragmoplast Secreted, extracellular exosome Note=In interphase, SEC3A localizes to the cytoplasm and plasma membrane, where it forms immobile, punctate structures. Localizes to the early cell plate and completed division wall during cytokinesis. Shuttles from the cytoplasm to the exocyst-positive organelle (EXPO) in the presence of EXO70E2. " Q9SX99,"PROTEIN NAMES: Protein OSB1, mitochondrial (Organellar single-stranded DNA-binding protein 1) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Regulates mitochondrial DNA recombination. Represses homologous recombination, preventing mitochondrial genome instability and unbalanced transmission of alternative mtDNA configurations. Binds preferentially single-stranded DNA. Does not bind to RNA. KEYWORDS: DNA-binding;Mitochondrion;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Mitochondrion " Q9SXA6,"PROTEIN NAMES: Endonuclease 1 (AtENDO1) (Bifunctional nuclease I) (AtBFN1) (Deoxyribonuclease ENDO1) (Single-stranded-nucleate endonuclease ENDO1) PROTEIN FAMILY: Nuclease type I family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the nuclease type I family. FUNCTION: Endonuclease that can use RNA, single-stranded and double-stranded DNA as substrates. Hydrolyzes single-stranded DNA and RNA without apparent specificity for bases during senescence. Endonuclease that recognizes and cleaves all types of mismatches with high efficiency, including heteroduplex double-stranded DNA. Maybe involved in programmed cell death (PCD) and senescence. KEYWORDS: Calcium;Disulfide bond;Endonuclease;Glycoprotein;Hydrolase;Manganese;Metal-binding;Nuclease;Reference proteome;Signal " Q9SXC4,"PROTEIN NAMES: Probable beta-1,4-xylosyltransferase IRX9H (Protein IRREGULAR XYLEM 9 homolog) (Xylan xylosyltransferase IRX9H) PROTEIN FAMILY: Glycosyltransferase 43 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the glycosyltransferase 43 family. FUNCTION: Involved in the synthesis of the hemicellulose glucuronoxylan, a major component of secondary cell walls. Probably involved in the elongation of glucuronoxylan xylosyl backbone. KEYWORDS: Cell wall biogenesis/degradation;Glycoprotein;Glycosyltransferase;Golgi apparatus;Membrane;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus membrane ; Single-pass type II membrane protein " Q9SXY0,"PROTEIN NAMES: Chromatin assembly factor 1 subunit FAS1 (CAF-1 subunit FAS1) (CAF-1 p150 homolog) (Protein FASCIATA 1) PROTEIN FAMILY: CHAF1A family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the CHAF1A family. FUNCTION: Component of the chromatin assembly factor complex (CAF-1) involved in chromatin assembly following DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. Required for several aspects of development, including seedling growth and leaf hair differentiation. Plays a critical role in the organization of shoot apical meristem (SAM) and root apical meristem (RAM) during postembryonic development by facilitating stable maintenance of gene expression states. Seems not required to maintain transcriptional repression of heterochromatic genes. Involved in heterologous recombination. May repress endocycle. KEYWORDS: Alternative splicing;Chromatin regulator;Coiled coil;DNA damage;DNA repair;DNA replication;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " Q9SYJ8,"PROTEIN NAMES: Protein SOSEKI 1 (AtSOK1) PROTEIN FAMILY: SOSEKI family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the SOSEKI family. FUNCTION: SOSEKI proteins (SOK1-5) locally interpret global polarity cues and can influence cell division orientation to coordinate cell polarization relative to body axes, probably by guiding ANGUSTIFOLIA (AN) polarized localization. KEYWORDS: Cell cycle;Cell division;Cell membrane;Developmental protein;Membrane;Reference proteome SUBCELLULAR LOCATION: Cell membrane ; Peripheral membrane protein ; Cytoplasmic side Note=SOSEKI proteins (SOK1-5) integrate apical-basal and radial organismal axes to localize to polar cell edges in roots, pointing towards the endodermis, mainly to inner apical edges. MISCELLANEOUS: 'Soseki' means cornerstone in Japanese." Q9SYL0,PROTEIN NAMES: BTB/POZ and TAZ domain-containing protein 3 (BTB and TAZ domain protein 3) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: May act as a substrate-specific adapter of an E3 ubiquitin-protein ligase complex (CUL3-RBX1-BTB) which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Essential for female and male gametophyte development. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Alternative splicing;Metal-binding;Reference proteome;Ubl conjugation pathway;Zinc;Zinc-finger MISCELLANEOUS: [Isoform 2]: May be due to an intron retention. Q9SYM0,"PROTEIN NAMES: Protein VTE6, chloroplastic (Phytyl-P kinase) (Vitamin E deficient 6) PROTEIN FAMILY: TMEM19 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the TMEM19 family. FUNCTION: Phytyl-phosphate kinase catalyzing the conversion of phytyl-monophosphate to phytyl-diphosphate. Involved in the activation and reutilization of phytol from chlorophyll degradation in plant metabolism, including tocopherol (vitamin E) biosynthesis. Involved in the biosynthesis of phylloquinone (vitamin K), which is required for the photosystem I (PSI) complex stability. PATHWAY: Cofactor biosynthesis; tocopherol biosynthesis. KEYWORDS: Alternative splicing;Chloroplast;Membrane;Plastid;Reference proteome;Transferase;Transit peptide;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Plastid, chloroplast membrane ; Multi-pass membrane protein MISCELLANEOUS: Overexpression of VTE6 results in increased phytyl-PP and tocopherol levels in seeds." Q9SYM5,"PROTEIN NAMES: Trifunctional UDP-glucose 4,6-dehydratase/UDP-4-keto-6-deoxy-D-glucose 3,5-epimerase/UDP-4-keto-L-rhamnose-reductase RHM1 (Protein REPRESSOR OF LRX1 1) (Rhamnose biosynthetic enzyme 1) (AtRHM1) [Includes: UDP-glucose 4,6-dehydratase ; UDP-4-keto-6-deoxy-D-glucose 3,5-epimerase/UDP-4-keto-L-rhamnose 4-keto-reductase ] PROTEIN FAMILY: NAD(P)-dependent epimerase/dehydratase family, dTDP-glucose dehydratase subfamily; DTDP-4-dehydrorhamnose reductase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: In the N-terminal section; belongs to the NAD(P)-dependent epimerase/dehydratase family. dTDP-glucose dehydratase subfamily.; SIMILARITY: In the C-terminal section; belongs to the dTDP-4-dehydrorhamnose reductase family. FUNCTION: Trifunctional enzyme involved in UDP-beta-L-rhamnose biosynthesis, a precursor of the primary cell wall components rhamnogalacturonan I (RG-I) and rhamnogalacturonan II (RG-II). Plays a major role in supplying UDP-rhamnose for flavonol biosynthesis. Catalyzes the dehydration of UDP-glucose to form UDP-4-dehydro-6-deoxy-D-glucose followed by the epimerization of the C3' and C5' positions of UDP-4-dehydro-6-deoxy-D-glucose to form UDP-4-keto-beta-L-rhamnose and the reduction of UDP-4-keto-beta-L-rhamnose to yield UDP-beta-L-rhamnose (By similarity). PATHWAY: Carbohydrate biosynthesis. KEYWORDS: Cell wall biogenesis/degradation;Cytoplasm;Isomerase;Lyase;Multifunctional enzyme;NAD;NADP;Oxidoreductase;Reference proteome SUBCELLULAR LOCATION: Cytoplasm, cytosol MISCELLANEOUS: The increased accumulation of auxin in rol1-2 seedlings appears to be caused by a flavonol-induced modification of auxin transport. In bacteria, TDP-L-rhamnose is formed by the successive action of three different enzymes on TDP-D-glucose. In plants, on the other hand, a single polypeptide probably catalyzes all three reactions that lead to the conversion of UDP-D-glucose to UDP-L-rhamnose." Q9SYU4,"PROTEIN NAMES: Peroxisome biogenesis factor 10 (PER10) (Peroxin-10) (Peroxisomal biogenesis factor 10) (AtPEX10) (AthPEX10) (Peroxisome assembly protein 10) (Pex10p) PROTEIN FAMILY: Pex2/pex10/pex12 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the pex2/pex10/pex12 family. FUNCTION: E3 ubiquitin-protein ligase component of a retrotranslocation channel required for peroxisome organization by mediating export of the PEX5 receptor from peroxisomes to the cytosol, thereby promoting PEX5 recycling. The retrotranslocation channel is composed of PEX2, PEX10 and PEX12; each subunit contributing transmembrane segments that coassemble into an open channel that specifically allows the passage of PEX5 through the peroxisomal membrane (By similarity). PEX10 also regulates PEX5 recycling by acting as a E3 ubiquitin-protein ligase. When PEX5 recycling is compromised, PEX10 catalyzes polyubiquitination of PEX5 during its passage through the retrotranslocation channel, leading to its degradation (By similarity). PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Membrane;Metal-binding;Peroxisome;Peroxisome biogenesis;Protein transport;Reference proteome;Transferase;Transmembrane;Transmembrane helix;Transport;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Peroxisome membrane ; Multi-pass membrane protein " Q9SYX1,"PROTEIN NAMES: Light-harvesting complex-like protein 3 isotype 1, chloroplastic (LHC-like protein 3 isoform 1) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Light-harvesting-like protein required for biosynthesis of phytylated chlorophylls and alpha-tocopherol in green seedlings. Functions by anchoring geranylgeranyl reductase (GGR) in the thylakoid membrane, leading to the stabilization of GGR activity. Binds chlorophyll a in the thylakoid membrane (By similarity). Plays a role in the regulation of chlorophyll biosynthesis under light stress and under standard growth conditions. KEYWORDS: Chlorophyll;Chloroplast;Chromophore;Membrane;Photosynthesis;Photosystem II;Plastid;Reference proteome;Thylakoid;Transit peptide;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane ; Multi-pass membrane protein Note=Associates with subcomplexes of LHC antenna of photosystem II. " Q9SZ45,"PROTEIN NAMES: Calcium uptake protein, mitochondrial (Mitochondrial calcium uniporter) PROTEIN FAMILY: MICU1 family, MICU1 subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the MICU1 family. MICU1 subfamily. FUNCTION: Calcium-binding protein maintaining matrix calcium levels at low concentration. Regulates mitochondrial calcium dynamics in planta by restricting influx. KEYWORDS: Alternative splicing;Calcium;Calcium transport;Ion transport;Membrane;Metal-binding;Mitochondrion;Mitochondrion inner membrane;Reference proteome;Repeat;Transit peptide;Transport SUBCELLULAR LOCATION: Mitochondrion inner membrane Mitochondrion intermembrane space Note=Located at the inner mitochondrial membrane and/or in the intermembrane space. " Q9SZ58,"PROTEIN NAMES: Brassinosteroid-related acyltransferase 1 (BR-related acyltransferase 1) (Protein DWARF AND ROUND LEAF 1) (Protein PIZZA) PROTEIN FAMILY: Plant acyltransferase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the plant acyltransferase family. FUNCTION: Brassinosteroids (BR) acyltransferase with acyl-CoA ligase activity toward brassinolide (BL), castasterone (CS), typhasterol (TY), 6-deoxotyphasterol (6-deoxoTY), and 6-deoxocastasterone (6-deoxoCS) and thus converts them to corresponding lauroyl esters. Regulates BR homeostasis and promotes BR-mediated cell growth regulation. Involved in vascular bundle development. PATHWAY: Plant hormone biosynthesis; brassinosteroid biosynthesis. KEYWORDS: Acyltransferase;Brassinosteroid biosynthesis;Endoplasmic reticulum;Lipid biosynthesis;Lipid metabolism;Nucleus;Reference proteome;Steroid biosynthesis;Transferase SUBCELLULAR LOCATION: Endoplasmic reticulum Nucleus Note=Localizes in the endoplasmic reticulum in young tissues and the phloem of vascular bundles. " Q9SZ63,"PROTEIN NAMES: Nudix hydrolase 14, chloroplastic (AtNUDT14) (ADP-sugar diphosphatase) (AtASPP) PROTEIN FAMILY: Nudix hydrolase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the Nudix hydrolase family. FUNCTION: Mediates the hydrolysis of some nucleoside diphosphate derivatives. Can use ADP-glucose, ADP-mannose and ADP-ribose as substrates. Regulates the intracellular ADP-glucose levels linked to starch biosynthesis. KEYWORDS: Chloroplast;Hydrolase;Magnesium;Manganese;Metal-binding;Plastid;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast " Q9SZH4,"PROTEIN NAMES: RNA-binding KH domain-containing protein PEPPER ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Regulates vegetative and gynoecium development. In concert with HUA2, antagonizes FLK by positively regulating FLC probably at transcriptional and post-transcriptional levels, and thus acts as a negative regulator of flowering. KEYWORDS: Nucleus;Reference proteome;Repeat;RNA-binding;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " Q9SZP1,"PROTEIN NAMES: Transcription repressor MYB4 (Myb-related protein 4) (AtMYB4) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Transcription repressor involved in regulation of protection against UV. Mediates transcriptional repression of CYP73A5, the gene encoding trans-cinnamate 4-monooxygenase, thereby regulating the accumulation of the UV-protectant compound sinapoylmalate. KEYWORDS: DNA-binding;Nucleus;Reference proteome;Repeat;Repressor;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " Q9SZV3,"PROTEIN NAMES: Folate synthesis bifunctional protein, mitochondrial [Includes: 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase (HPPK) (2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase) (7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase); Dihydropteroate synthase (DHPS) ] PROTEIN FAMILY: HPPK family; DHPS family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: In the N-terminal section; belongs to the HPPK family.; SIMILARITY: In the C-terminal section; belongs to the DHPS family. FUNCTION: Catalyzes the first two consecutive steps of tetrahydrofolate biosynthesis. PATHWAY: Cofactor biosynthesis; tetrahydrofolate biosynthesis; 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate from 7,8-dihydroneopterin triphosphate: step 4/4.; PATHWAY: Cofactor biosynthesis; tetrahydrofolate biosynthesis; 7,8-dihydrofolate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate and 4-aminobenzoate: step 1/2. KEYWORDS: Alternative splicing;ATP-binding;Folate biosynthesis;Kinase;Magnesium;Metal-binding;Mitochondrion;Multifunctional enzyme;Nucleotide-binding;Reference proteome;Transferase;Transit peptide SUBCELLULAR LOCATION: Mitochondrion " Q9T003,"PROTEIN NAMES: Acetylserotonin O-methyltransferase (N-acetylserotonin O-methyltransferase) (AtASMT) PROTEIN FAMILY: Class I-like SAM-binding methyltransferase superfamily, Cation-independent O-methyltransferase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O-methyltransferase family. FUNCTION: Methyltransferase which catalyzes the transfer of a methyl group onto N-acetylserotonin, producing melatonin (N-acetyl-5-methoxytryptamine). Does not seem to possess caffeate O-methyltransferase activity. Implicated in melatonin-dependent circadian dynamics of stomatal aperture to minimize night water loss and promote drought tolerance. Prevents seed germination by promoting melatonin biosynthesis. Promotes melatonin-triggered defense responses to the necrotrophic fungus Botrytis cinerea.; FUNCTION: (Microbial infection) Promotes melatonin-triggered defense responses to the necrotrophic fungus Botrytis cinerea. PATHWAY: Aromatic compound metabolism; melatonin biosynthesis; melatonin from serotonin: step 1/2. KEYWORDS: Cytoplasm;Melatonin biosynthesis;Methyltransferase;Reference proteome;S-adenosyl-L-methionine;Transferase SUBCELLULAR LOCATION: Cytoplasm " Q9T071,PROTEIN NAMES: Probable hexokinase-like 2 protein PROTEIN FAMILY: Hexokinase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the hexokinase family. FUNCTION: Fructose and glucose phosphorylating enzyme. PATHWAY: Carbohydrate metabolism; hexose metabolism.; PATHWAY: Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 1/4. KEYWORDS: ATP-binding;Glycolysis;Kinase;Membrane;Nucleotide-binding;Reference proteome;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Single-pass membrane protein Q9T0H9,"PROTEIN NAMES: Protein PAM71-homolog, chloroplastic (GDT1-like protein 2) (PHOTOSYNTHESIS AFFECTED MUTANT71-homolog) PROTEIN FAMILY: GDT1 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the GDT1 family. FUNCTION: Probable chloroplast-localized Mn(2+)/H(+) and/or Ca(2+)/H(+) antiporter regulating Ca(2+), Mn(2+) and pH homeostasis. KEYWORDS: Alternative splicing;Chloroplast;Membrane;Plastid;Reference proteome;Transit peptide;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Plastid, chloroplast membrane ; Multi-pass membrane protein " Q9TVL3,"PROTEIN NAMES: Probable cyclin-dependent kinase 9 (Cell division protein kinase 9) PROTEIN FAMILY: Protein kinase superfamily, CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. FUNCTION: Essential member of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor B (P-TEFb), which is proposed to facilitate the transition from abortive to production elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II) and spt-5. KEYWORDS: Alternative splicing;ATP-binding;Kinase;Nucleotide-binding;Nucleus;Reference proteome;Serine/threonine-protein kinase;Transcription;Transcription regulation;Transferase SUBCELLULAR LOCATION: Nucleus " Q9TVY6,"PROTEIN NAMES: Sperm transmembrane protein 9 (Fertilization defective 9) (Spermatogenesis defective 9) ORGANISM: Caenorhabditis elegans FUNCTION: Required for fertilization. May be required for cell adhesion and/or function as a signaling molecule. KEYWORDS: Alternative splicing;Cell adhesion;Cytoplasm;Disulfide bond;EGF-like domain;Fertilization;Glycoprotein;Membrane;Reference proteome;Repeat;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cytoplasm Membrane ; Single-pass type I membrane protein MISCELLANEOUS: Removing the EGF-like repeats in any combination completely abolishes fertilization.; MISCELLANEOUS: Modifying each EGF repeat by changing a central cysteine residue to a tyrosine disrupts the secondary structure as the specific disulfide bridges responsible for this overall structure are not formed. The cysteine to tyrosine mutation when located in EGF-like domains 1,3,4 and 5, causes a complete loss of function. The same mutation in additional EGF-like domains leads to a temperature sensitive sterile phenotype." Q9TYT3,"PROTEIN NAMES: SHC-transforming protein homolog 1 (Src homology 2 domain adapter homolog 1) ORGANISM: Caenorhabditis elegans FUNCTION: Scaffold protein which plays an important role in the activation of the JNK pathway composed of mlk-1, mek-1 and kgb-1; by bringing together mek-1 and mlk-1, promotes mlk-1-mediated phosphorylation and activation of mek-1 which in turn phosphorylates kgb-1. In addition, negatively modulates the activation of the insulin/IGF-1-like signaling (IIS) probably by inhibiting the insulin receptor daf-2. Positively regulates the activity of the transcription factor daf-16/FOXO by both inhibiting IIS and activating the JNK pathway. Plays a role in maintaining gonadal basement membrane integrity through activation of the JNK pathway components mek-1 and jnk-1. Involved in the response to several environmental stresses including heavy metal ions (Cu(2+) and Cd(2+)), heat, oxidative and protein misfolding (ER) stresses. Plays a role in gonad and germline development following the L1 diapause. Plays a role in life span and egg laying. Plays a role in axon regeneration after injury. KEYWORDS: Alternative splicing;Cell membrane;Cytoplasm;Membrane;Nucleus;Reference proteome;SH2 domain;Stress response SUBCELLULAR LOCATION: Cytoplasm Nucleus Cell membrane ; Peripheral membrane protein Note=In intestinal cells, enriched in the nucleus. " Q9TYV5,"PROTEIN NAMES: 26S rRNA (cytosine-C(5))-methyltransferase nsun-1 (5-methylcytosine rRNA methyltransferase nsun-1) (NOL1/NOP2/Sun domain family member 1) (RNA cytosine C(5)-methyltransferase nsun-1) (rRNA cytosine C(5)-methyltransferase nsun-1) PROTEIN FAMILY: Class I-like SAM-binding methyltransferase superfamily, RsmB/NOP family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family. FUNCTION: Methyltransferase which methylates the carbon-5 position of cytosine 2982 to 5-methylcytosine (m5C2982) in 26S rRNA. May play a role in the translation of leucine and proline codons (Probable). May be required for the translation of specific mRNAs such as mRNAs involved in gonad development, collagen production and cuticle integrity. Plays a role in ensuring the correct localization of the germline-specific protein gld-1 during development. Not required for pre-rRNA processing, the production of mature 5S, 5.8S, 18S or 26S rRNAs or global translation. Plays a role in positively regulating fertility. KEYWORDS: Alternative splicing;Methyltransferase;Nucleus;Reference proteome;Ribosome biogenesis;RNA-binding;rRNA processing;S-adenosyl-L-methionine;Transferase SUBCELLULAR LOCATION: Nucleus, nucleolus " Q9TZP5,"PROTEIN NAMES: Putative inactive caspase B [Cleaved into: Putative inactive caspase B subunit p31; Putative inactive caspase B subunit p17; Putative inactive caspase subunit p14] PROTEIN FAMILY: Peptidase C14A family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the peptidase C14A family. FUNCTION: [Isoform b]: Putative inactive caspase. In the germline, binds caspase ced-3 zymogen and prevents ced-3 autoactivation. Does not affect the caspase activity of mature ced-3 and ced-4-mediated mature ced-3 activation. Negatively regulates germline apoptosis by inhibiting autocleavage of caspase ced-3. Involved in fertility.; FUNCTION: [Isoform a]: Putative inactive caspase. Dispensable for the inhibition of germline apoptosis. KEYWORDS: Alternative splicing;Cytoplasm;Reference proteome SUBCELLULAR LOCATION: [Isoform b]: Cytoplasm " Q9U1H9,"PROTEIN NAMES: Nuclear RNA export factor 1 (Protein small bristles) (Protein tip-associating) PROTEIN FAMILY: NXF family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the NXF family. FUNCTION: Mediates the export of the majority of mRNAs from the nucleus to the cytoplasm. In ovarian follicle cells, plays a role in transposable element silencing regulation by enabling the nuclear export of flamenco (flam) transcripts and subsequent piRNA biogenesis. KEYWORDS: 3D-structure;Cytoplasm;Leucine-rich repeat;mRNA transport;Nucleus;Phosphoprotein;Reference proteome;Repeat;RNA-binding;Transport SUBCELLULAR LOCATION: Nucleus, nucleoplasm Cytoplasm Nucleus envelope Note=Localized in the nucleoplasm and at both the nucleoplasmic and cytoplasmic faces of the nuclear pore complex. Shuttles between the nucleus and the cytoplasm. MISCELLANEOUS: Mutations affect the morphogenesis of embryonic neurons, embryonic muscle and adult sensory bristles. This is thought to be due to reduced rate of protein synthesis as the mRNA is not efficiently being exported out of the nucleus." Q9U1M9,"PROTEIN NAMES: Dynamin-B PROTEIN FAMILY: TRAFAC class dynamin-like GTPase superfamily, Dynamin/Fzo/YdjA family ORGANISM: Dictyostelium discoideum (Social amoeba) SIMILARITY: Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family. FUNCTION: Enzyme hydrolyzing GTP. KEYWORDS: Coiled coil;Cytoplasm;GTP-binding;Hydrolase;Nucleotide-binding;Reference proteome SUBCELLULAR LOCATION: Cytoplasm " Q9U221,"PROTEIN NAMES: Uracil-DNA glycosylase (UDG) PROTEIN FAMILY: Uracil-DNA glycosylase (UDG) superfamily, UNG family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the uracil-DNA glycosylase (UDG) superfamily. UNG family. FUNCTION: Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine. KEYWORDS: Alternative splicing;DNA damage;DNA repair;Hydrolase;Mitochondrion;Nucleus;Reference proteome SUBCELLULAR LOCATION: Mitochondrion Nucleus " Q9U2M5,"PROTEIN NAMES: G1/S-specific cyclin-D PROTEIN FAMILY: Cyclin family, Cyclin D subfamily ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the cyclin family. Cyclin D subfamily. FUNCTION: In association with cdk-4, regulates the progression through the G1 phase of the cell cycle during postembryonic development. Regulates proliferation of the coelomocyte lineage and intestinal cells during late embryogenesis. In complex with cdk-4, involved in sex determination during gonadogenesis by regulating the asymmetric division of the somatic gonadal precursor cell (SGP). KEYWORDS: Cell cycle;Cell division;Cyclin;Differentiation;Gonadal differentiation;Reference proteome " Q9U3D6,"PROTEIN NAMES: A-to-I RNA editing regulator adr-1 ORGANISM: Caenorhabditis elegans FUNCTION: Required for the hydrolytic deamination of adenosine to inosine in double-stranded RNA (dsRNA) referred to as A-to-I RNA editing. Does not have deaminase activity but binds to dsRNA and regulates A-to-I RNA editing, possibly by modulating the accessibility of the double-stranded RNA-specific adenosine deaminase adr-2 for target adenosines. Regulates editing efficiency of specific adenosines in 3' untranslated regions of target mRNAs. Represses editing efficiency of the Y75B8A.8 3' UTR in neurons. Required for correct expression and A-to-I editing of clec-41. Required for normal chemotaxis. Also required for normal vulva development. Plays a role in determining lifespan. Not required for RNA interference. Likely to play a role in determining whether a dsRNA enters the RNAi pathway. KEYWORDS: Alternative splicing;Chemotaxis;Cytoplasm;mRNA processing;Nucleus;Reference proteome;RNA-binding SUBCELLULAR LOCATION: Nucleus Cytoplasm Note=Detected both in the nuclear fraction and in the ribosome-containing pellet of the cytoplasm. MISCELLANEOUS: Can prevent somatic transgenes from inducing gene silencing via the RNA interference pathway. This may occur due to A-to-I editing of transgene-derived dsRNA, preventing transgene RNAi." Q9U405,PROTEIN NAMES: Transcription factor grauzone ORGANISM: Drosophila melanogaster (Fruit fly) FUNCTION: Transcription factor essential for the completion of meiosis in oocytes. Grauzone binds to the promoter region of cort via the zf-AD domain and activates cort expression in ovaries. KEYWORDS: 3D-structure;Activator;DNA-binding;Meiosis;Metal-binding;Nucleus;Reference proteome;Repeat;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Q9U6A0,"PROTEIN NAMES: Sodium/potassium/calcium exchanger Nckx30C (Na(+)/K(+)/Ca(2+)-exchange protein Nckx30C) PROTEIN FAMILY: Ca(2+):cation antiporter (CaCA) family, SLC24A subfamily ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19) family. SLC24A subfamily. FUNCTION: May function in the removal and maintenance of calcium homeostasis during signaling in the adult and in signaling events during embryogenesis and patterning of imaginal disks. Transports one Ca(2+) and 1 K(+) in exchange for 4 Na(+). KEYWORDS: Alternative splicing;Antiport;Calcium;Calcium transport;Glycoprotein;Ion transport;Membrane;Potassium;Potassium transport;Reference proteome;Repeat;RNA editing;Sodium;Sodium transport;Symport;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. " Q9U6M1,"PROTEIN NAMES: Thymine dioxygenase JBP1 (J-binding protein 1) (Thymidine hydroxylase JBP1) PROTEIN FAMILY: TET family, JBP1 subfamily ORGANISM: Leishmania tarentolae (Sauroleishmania tarentolae) SIMILARITY: Belongs to the TET family. JBP1 subfamily. FUNCTION: Dioxygenase that catalyzes the first step of DNA base J (beta-d-glucosyl-HOMedU) biosynthesis by converting thymine to 5-hydroxymethyluracil (HOMedU). DNA base J is a hypermodified thymidine residue found in the genome of kinetoplastid parasites, which is localized primarily to repetitive DNA, namely the telomeres, and is implicated in the regulation of antigenic variation. Also specifically binds to base J-containing DNA (J-DNA). Involved in propagation and maintenance of DNA base J synthesis initiated by JBP2 by specifically binding already synthesized DNA base J and propagating J synthesis. Thymine dioxygenase activity and J-DNA-binding are independent functions. KEYWORDS: 3D-structure;Dioxygenase;DNA-binding;Iron;Metal-binding;Nucleus;Oxidoreductase SUBCELLULAR LOCATION: Nucleus Note=Localizes to discrete spots in the nucleus, probably the J-rich telomeres. " Q9U8W8,"PROTEIN NAMES: Techylectin-5A ORGANISM: Tachypleus tridentatus (Japanese horseshoe crab) FUNCTION: Lectin involved in innate immunity. Agglutinates all types of human erythrocytes, Gram-positive and Gram-negative bacteria. Has a stronger agglutinating activity towards Gram-negative bacteria than towards Gram-positive bacteria. Specifically recognizes acetyl group-containing substances on agglutinated cells. The hemagglutinating activity was inhibited by EDTA, acetyl group-containing mono- and disaccharides, N-acetyl derivatives of amino acids, other acetyl group-containing substances, propionamide and benzamide. Enhances the antimicrobial activity of big defensin against Gram-positive bacteria but not against Gram-negative bacteria. KEYWORDS: 3D-structure;Calcium;Direct protein sequencing;Disulfide bond;Glycoprotein;Lectin;Metal-binding;Pyrrolidone carboxylic acid;Secreted;Signal SUBCELLULAR LOCATION: Secreted. " Q9UAS6,PROTEIN NAMES: Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 2 (Cap methyltransferase 2 homolog) (Cap2 2'O-ribose methyltransferase 2 homolog) (MTr2) (FtsJ methyltransferase domain-containing protein 1 homolog) (Protein Adrift) ORGANISM: Drosophila melanogaster (Fruit fly) FUNCTION: Probable S-adenosyl-L-methionine-dependent methyltransferase that mediates mRNA cap2 2'-O-ribose methylation to the 5'-cap structure of mRNAs. May methylate the ribose of the second nucleotide of a m(7)GpppG-capped mRNA (cap0) to produce m(7)GpppRmpNm (cap2) (By similarity). Regulates expression of tracheal genes required for pathfinding on the segmental nerve. KEYWORDS: Methyltransferase;mRNA capping;mRNA processing;Nucleus;Reference proteome;S-adenosyl-L-methionine;Transferase SUBCELLULAR LOCATION: Nucleus Q9UBD0,"PROTEIN NAMES: Heat shock transcription factor, X-linked PROTEIN FAMILY: HSF family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the HSF family. KEYWORDS: Cytoplasm;DNA-binding;Isopeptide bond;Nucleus;Reference proteome;Transcription;Transcription regulation;Ubl conjugation SUBCELLULAR LOCATION: Nucleus Cytoplasm " Q9UBV8,"PROTEIN NAMES: Peflin (PEF protein with a long N-terminal hydrophobic domain) (Penta-EF hand domain-containing protein 1) ORGANISM: Homo sapiens (Human) FUNCTION: Calcium-binding protein that acts as an adapter that bridges unrelated proteins or stabilizes weak protein-protein complexes in response to calcium. Together with PDCD6, acts as a calcium-dependent adapter for the BCR(KLHL12) complex, a complex involved in endoplasmic reticulum (ER)-Golgi transport by regulating the size of COPII coats. In response to cytosolic calcium increase, the heterodimer formed with PDCD6 interacts with, and bridges together the BCR(KLHL12) complex and SEC31 (SEC31A or SEC31B), promoting monoubiquitination of SEC31 and subsequent collagen export, which is required for neural crest specification. Its role in the heterodimer formed with PDCD6 is however unclear: some evidence shows that PEF1 and PDCD6 work together and promote association between PDCD6 and SEC31 in presence of calcium. Other reports show that PEF1 dissociates from PDCD6 in presence of calcium, and may act as a negative regulator of PDCD6. Also acts as a negative regulator of ER-Golgi transport; possibly by inhibiting interaction between PDCD6 and SEC31 (By similarity). KEYWORDS: Calcium;Cytoplasm;Cytoplasmic vesicle;Endoplasmic reticulum;Isopeptide bond;Membrane;Metal-binding;Reference proteome;Repeat;Ubl conjugation SUBCELLULAR LOCATION: Cytoplasm Endoplasmic reticulum Membrane ; Peripheral membrane protein Cytoplasmic vesicle, COPII-coated vesicle membrane ; Peripheral membrane protein Note=Membrane-associated in the presence of Ca(2+). Localizes to endoplasmic reticulum exit site (ERES) (By similarity). " Q9UBW5,"PROTEIN NAMES: Bridging integrator 2 (Breast cancer-associated protein 1) ORGANISM: Homo sapiens (Human) FUNCTION: Promotes cell motility and migration, probably via its interaction with the cell membrane and with podosome proteins that mediate interaction with the cytoskeleton. Modulates membrane curvature and mediates membrane tubulation. Plays a role in podosome formation. Inhibits phagocytosis. KEYWORDS: 3D-structure;Alternative splicing;Cell junction;Cell membrane;Cell projection;Coiled coil;Cytoplasm;Membrane;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Cell projection, podosome membrane ; Peripheral membrane protein ; Cytoplasmic side Cytoplasm, cell cortex Cell projection, phagocytic cup Note=Associates with membranes enriched in phosphoinositides. Detected in the actin-rich cell cortex at the leading edge of migrating cells. Detected at podosomes, at an actin-rich ring-like structure. " Q9UBX1,PROTEIN NAMES: Cathepsin F (CATSF) PROTEIN FAMILY: Peptidase C1 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the peptidase C1 family. FUNCTION: Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis. KEYWORDS: 3D-structure;Disease variant;Disulfide bond;Glycoprotein;Hydrolase;Lysosome;Neurodegeneration;Neuronal ceroid lipofuscinosis;Protease;Reference proteome;Signal;Thiol protease;Zymogen SUBCELLULAR LOCATION: Lysosome. Q9UDV6,PROTEIN NAMES: Zinc finger protein 212 (Zinc finger protein C2H2-150) PROTEIN FAMILY: Krueppel C2H2-type zinc-finger protein family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein family. FUNCTION: May be involved in transcriptional regulation. KEYWORDS: DNA-binding;Metal-binding;Nucleus;Reference proteome;Repeat;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Q9UDX3,"PROTEIN NAMES: SEC14-like protein 4 (Tocopherol-associated protein 3) ORGANISM: Homo sapiens (Human) FUNCTION: Probable hydrophobic ligand-binding protein; may play a role in the transport of hydrophobic ligands like tocopherol, squalene and phospholipids. KEYWORDS: 3D-structure;Alternative splicing;Lipid-binding;Reference proteome;Transport " Q9UF12,"PROTEIN NAMES: Hydroxyproline dehydrogenase (HYPDH) (Kidney and liver proline oxidase 1) (HsPOX1) (Probable proline dehydrogenase 2) (Probable proline oxidase 2) PROTEIN FAMILY: Proline oxidase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the proline oxidase family. FUNCTION: Dehydrogenase that converts trans-4-L-hydroxyproline to delta-1-pyrroline-3-hydroxy-5-carboxylate (Hyp) using ubiquinone-10 as the terminal electron acceptor. Can also use proline as a substrate but with a very much lower efficiency. Does not react with other diastereomers of Hyp: trans-4-D-hydroxyproline and cis-4-L-hydroxyproline. Ubiquininone analogs such as menadione, duroquinone and ubiquinone-1 react more efficiently than oxygen as the terminal electron acceptor during catalysis. KEYWORDS: Acetylation;FAD;Flavoprotein;Oxidoreductase;Proline metabolism;Reference proteome " Q9UGN5,"PROTEIN NAMES: Poly [ADP-ribose] polymerase 2 (PARP-2) (hPARP-2) (ADP-ribosyltransferase diphtheria toxin-like 2) (ARTD2) (DNA ADP-ribosyltransferase PARP2) (NAD(+) ADP-ribosyltransferase 2) (ADPRT-2) (Poly[ADP-ribose] synthase 2) (pADPRT-2) (Protein poly-ADP-ribosyltransferase PARP2) PROTEIN FAMILY: ARTD/PARP family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the ARTD/PARP family. FUNCTION: Poly-ADP-ribosyltransferase that mediates poly-ADP-ribosylation of proteins and plays a key role in DNA repair. Mediates glutamate, aspartate or serine ADP-ribosylation of proteins: the ADP-D-ribosyl group of NAD(+) is transferred to the acceptor carboxyl group of target residues and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units. Serine ADP-ribosylation of proteins constitutes the primary form of ADP-ribosylation of proteins in response to DNA damage. Mediates glutamate and aspartate ADP-ribosylation of target proteins in absence of HPF1. Following interaction with HPF1, catalyzes serine ADP-ribosylation of target proteins; HPF1 conferring serine specificity by completing the PARP2 active site. PARP2 initiates the repair of double-strand DNA breaks: recognizes and binds DNA breaks within chromatin and recruits HPF1, licensing serine ADP-ribosylation of target proteins, such as histones, thereby promoting decompaction of chromatin and the recruitment of repair factors leading to the reparation of DNA strand breaks. HPF1 initiates serine ADP-ribosylation but restricts the polymerase activity of PARP2 in order to limit the length of poly-ADP-ribose chains. Specifically mediates formation of branched poly-ADP-ribosylation. Branched poly-ADP-ribose chains are specifically recognized by some factors, such as APLF. In addition to proteins, also able to ADP-ribosylate DNA: preferentially acts on 5'-terminal phosphates at DNA strand breaks termini in nicked duplex. KEYWORDS: 3D-structure;Acetylation;ADP-ribosylation;Allosteric enzyme;Alternative splicing;Chromosome;DNA damage;DNA repair;DNA-binding;Glycosyltransferase;NAD;Nucleotidyltransferase;Nucleus;Phosphoprotein;Reference proteome;Transferase SUBCELLULAR LOCATION: Nucleus Chromosome Note=Recruited to DNA damage sites in a PARP1-dependent process: recognizes and binds poly-ADP-ribose chains produced by PARP1 at DNA damage sites via its N-terminus, leading to its recruitment. " Q9UGP4,"PROTEIN NAMES: LIM domain-containing protein 1 PROTEIN FAMILY: Zyxin/ajuba family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the zyxin/ajuba family. FUNCTION: Adapter or scaffold protein which participates in the assembly of numerous protein complexes and is involved in several cellular processes such as cell fate determination, cytoskeletal organization, repression of gene transcription, cell-cell adhesion, cell differentiation, proliferation and migration. Positively regulates microRNA (miRNA)-mediated gene silencing and is essential for P-body formation and integrity. Acts as a hypoxic regulator by bridging an association between the prolyl hydroxylases and VHL enabling efficient degradation of HIF1A. Acts as a transcriptional corepressor for SNAI1- and SNAI2/SLUG-dependent repression of E-cadherin transcription. Negatively regulates the Hippo signaling pathway and antagonizes phosphorylation of YAP1. Inhibits E2F-mediated transcription, and suppresses the expression of the majority of genes with E2F1-responsive elements. Regulates osteoblast development, function, differentiation and stress osteoclastogenesis. Enhances the ability of TRAF6 to activate adapter protein complex 1 (AP-1) and negatively regulates the canonical Wnt receptor signaling pathway in osteoblasts. May act as a tumor suppressor by inhibiting cell proliferation. KEYWORDS: Cell junction;Cytoplasm;LIM domain;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Repeat;Repressor;RNA-mediated gene silencing;Transcription;Transcription regulation;Tumor suppressor;Zinc SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Cytoplasm, P-body. Cell junction, adherens junction. Cell junction, focal adhesion. Note=Shuttles between cytoplasm and nucleus but is localized predominantly to the cytoplasm. Found in the nucleus but not nucleoli. Colocalizes with VCL in the focal adhesions. Down-regulation and/or elimination of its expression from the nucleus of neoplastic cells correlates strongly with poor patient prognosis and aggressive forms of breast carcinoma. Conversely, strong nuclear localization correlates with low-tumor grade and better patient prognosis. " Q9UGU5,PROTEIN NAMES: HMG domain-containing protein 4 (HMG box-containing protein 4) (High mobility group protein 2-like 1) (Protein HMGBCG) ORGANISM: Homo sapiens (Human) FUNCTION: Negatively regulates Wnt/beta-catenin signaling during development. KEYWORDS: DNA-binding;Isopeptide bond;Nucleus;Phosphoprotein;Reference proteome;Ubl conjugation;Wnt signaling pathway SUBCELLULAR LOCATION: Nucleus Q9UHD2,"PROTEIN NAMES: Serine/threonine-protein kinase TBK1 (NF-kappa-B-activating kinase) (T2K) (TANK-binding kinase 1) PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family, I-kappa-B kinase subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily. FUNCTION: Serine/threonine kinase that plays an essential role in regulating inflammatory responses to foreign agents. Following activation of toll-like receptors by viral or bacterial components, associates with TRAF3 and TANK and phosphorylates interferon regulatory factors (IRFs) IRF3 and IRF7 as well as DDX3X. This activity allows subsequent homodimerization and nuclear translocation of the IRFs leading to transcriptional activation of pro-inflammatory and antiviral genes including IFNA and IFNB. In order to establish such an antiviral state, TBK1 form several different complexes whose composition depends on the type of cell and cellular stimuli. Plays a key role in IRF3 activation: acts by first phosphorylating innate adapter proteins MAVS, STING1 and TICAM1 on their pLxIS motif, leading to recruitment of IRF3, thereby licensing IRF3 for phosphorylation by TBK1. Phosphorylated IRF3 dissociates from the adapter proteins, dimerizes, and then enters the nucleus to induce expression of interferons. Thus, several scaffolding molecules including FADD, TRADD, MAVS, AZI2, TANK or TBKBP1/SINTBAD can be recruited to the TBK1-containing-complexes. Under particular conditions, functions as a NF-kappa-B effector by phosphorylating NF-kappa-B inhibitor alpha/NFKBIA, IKBKB or RELA to translocate NF-Kappa-B to the nucleus. Restricts bacterial proliferation by phosphorylating the autophagy receptor OPTN/Optineurin on 'Ser-177', thus enhancing LC3 binding affinity and antibacterial autophagy. Phosphorylates SMCR8 component of the C9orf72-SMCR8 complex, promoting autophagosome maturation. Phosphorylates ATG8 proteins MAP1LC3C and GABARAPL2, thereby preventing their delipidation and premature removal from nascent autophagosomes. Phosphorylates and activates AKT1. Seems to play a role in energy balance regulation by sustaining a state of chronic, low-grade inflammation in obesity, wich leads to a negative impact on insulin sensitivity (By similarity). Attenuates retroviral budding by phosphorylating the endosomal sorting complex required for transport-I (ESCRT-I) subunit VPS37C. Phosphorylates Borna disease virus (BDV) P protein. Plays an essential role in the TLR3- and IFN-dependent control of herpes virus HSV-1 and HSV-2 infections in the central nervous system. Acts both as a positive and negative regulator of the mTORC1 complex, depending on the context: activates mTORC1 in response to growth factors by catalyzing phosphorylation of MTOR, while it limits the mTORC1 complex by promoting phosphorylation of RPTOR. KEYWORDS: 3D-structure;Amyotrophic lateral sclerosis;Antiviral defense;ATP-binding;Coiled coil;Cytoplasm;Disease variant;Glaucoma;Host-virus interaction;Immunity;Innate immunity;Isopeptide bond;Kinase;Neurodegeneration;Nucleotide-binding;Phosphoprotein;Reference proteome;Serine/threonine-protein kinase;Transferase;Ubl conjugation SUBCELLULAR LOCATION: Cytoplasm Note=Upon mitogen stimulation or triggering of the immune system, TBK1 is recruited to the exocyst by EXOC2. MISCELLANEOUS: In cancer cells, pathological TBK1 activation promotes oncogenic transformation by suppressing programmed cell death. Mechanistically, the RALB-SEC5/EXOC2-TBK1 signaling cascade seems to participate in both innate immune signaling and cell transformation. Additionally, TBK1 supports oncogenesis by directly phosphorylating and activating AKT1 at the exocyst." Q9UHF4,"PROTEIN NAMES: Interleukin-20 receptor subunit alpha (IL-20 receptor subunit alpha) (IL-20R-alpha) (IL-20RA) (Cytokine receptor class-II member 8) (Cytokine receptor family 2 member 8) (CRF2-8) (IL-20R1) (ZcytoR7) PROTEIN FAMILY: Type II cytokine receptor family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the type II cytokine receptor family. FUNCTION: The IL20RA/IL20RB dimer is a receptor for IL19, IL20 and IL24. The IL20RA/IL10RB dimer is a receptor for IL26. KEYWORDS: 3D-structure;Alternative splicing;Direct protein sequencing;Disulfide bond;Glycoprotein;Membrane;Receptor;Reference proteome;Repeat;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Single-pass type I membrane protein " Q9UHJ6,PROTEIN NAMES: Sedoheptulokinase (SHK) (Carbohydrate kinase-like protein) PROTEIN FAMILY: FGGY kinase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the FGGY kinase family. FUNCTION: Acts as a modulator of macrophage activation through control of glucose metabolism. KEYWORDS: ATP-binding;Cytoplasm;Kinase;Nucleotide-binding;Reference proteome;Transferase SUBCELLULAR LOCATION: Cytoplasm Q9UHP6,"PROTEIN NAMES: Radial spoke head 14 homolog (Rhabdoid tumor deletion region protein 1) PROTEIN FAMILY: Flagellar radial spoke RSP14 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the flagellar radial spoke RSP14 family. FUNCTION: Functions as part of axonemal radial spoke complexes that play an important part in the motility of sperm and cilia. KEYWORDS: 3D-structure;Cell projection;Cilium;Cytoplasm;Cytoskeleton;Flagellum;Reference proteome;Repeat SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, flagellum axoneme " Q9UIB8,"PROTEIN NAMES: SLAM family member 5 (Cell surface antigen MAX.3) (Hly9-beta) (Leukocyte differentiation antigen CD84) (Signaling lymphocytic activation molecule 5) (CD antigen CD84) ORGANISM: Homo sapiens (Human) FUNCTION: Self-ligand receptor of the signaling lymphocytic activation molecule (SLAM) family. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. Activities are controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2. Can mediate natural killer (NK) cell cytotoxicity dependent on SH2D1A and SH2D1B (By similarity). Increases proliferative responses of activated T-cells and SH2D1A/SAP does not seem be required for this process. Homophilic interactions enhance interferon gamma/IFNG secretion in lymphocytes and induce platelet stimulation via a SH2D1A-dependent pathway. May serve as a marker for hematopoietic progenitor cells Required for a prolonged T-cell:B-cell contact, optimal T follicular helper function, and germinal center formation. In germinal centers involved in maintaining B-cell tolerance and in preventing autoimmunity (By similarity). In mast cells negatively regulates high affinity immunoglobulin epsilon receptor signaling; independent of SH2D1A and SH2D1B but implicating FES and PTPN6/SHP-1. In macrophages enhances LPS-induced MAPK phosphorylation and NF-kappaB activation and modulates LPS-induced cytokine secretion; involving ITSM 2 (By similarity). Positively regulates macroautophagy in primary dendritic cells via stabilization of IRF8; inhibits TRIM21-mediated proteasomal degradation of IRF8. KEYWORDS: 3D-structure;Adaptive immunity;Alternative splicing;Autophagy;Cell adhesion;Cell membrane;Direct protein sequencing;Disulfide bond;Glycoprotein;Immunity;Immunoglobulin domain;Innate immunity;Membrane;Phosphoprotein;Receptor;Reference proteome;Repeat;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein " Q9UIC8,"PROTEIN NAMES: Leucine carboxyl methyltransferase 1 (Protein-leucine O-methyltransferase) ([Phosphatase 2A protein]-leucine-carboxy methyltransferase 1) PROTEIN FAMILY: Methyltransferase superfamily, LCMT family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the methyltransferase superfamily. LCMT family. FUNCTION: Methylates the carboxyl group of the C-terminal leucine residue of protein phosphatase 2A catalytic subunits to form alpha-leucine ester residues. KEYWORDS: 3D-structure;Alternative splicing;Methyltransferase;Reference proteome;S-adenosyl-L-methionine;Transferase " Q9UIF7,PROTEIN NAMES: Adenine DNA glycosylase (MutY homolog) (hMYH) PROTEIN FAMILY: Nth/MutY family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the Nth/MutY family. FUNCTION: Involved in oxidative DNA damage repair. Initiates repair of A*oxoG to C*G by removing the inappropriately paired adenine base from the DNA backbone. Possesses both adenine and 2-OH-A DNA glycosylase activities. KEYWORDS: 3D-structure;4Fe-4S;Alternative splicing;Disease variant;DNA damage;DNA repair;Glycosidase;Hydrolase;Iron;Iron-sulfur;Metal-binding;Mitochondrion;Nucleus;Reference proteome;Tumor suppressor SUBCELLULAR LOCATION: Nucleus Mitochondrion Q9UIU6,"PROTEIN NAMES: Homeobox protein SIX4 (Sine oculis homeobox homolog 4) PROTEIN FAMILY: SIX/Sine oculis homeobox family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the SIX/Sine oculis homeobox family. FUNCTION: Transcriptional regulator which can act as both a transcriptional repressor and activator by binding a DNA sequence on these target genes and is involved in processes like cell differentiation, cell migration and cell survival. Transactivates gene expression by binding a 5'-[CAT]A[CT][CT][CTG]GA[GAT]-3' motif present in the Trex site and a 5'-TCA[AG][AG]TTNC-3' motif present in the MEF3 site of the muscle-specific genes enhancer. Acts cooperatively with EYA proteins to transactivate their target genes through interaction and nuclear translocation of EYA protein. Acts synergistically with SIX1 to regulate target genes involved in formation of various organs, including muscle, kidney, gonad, ganglia, olfactory epithelium and cranial skeleton. Plays a role in several important steps of muscle development. Controls the genesis of hypaxial myogenic progenitors in the dermomyotome by transactivating PAX3 and the delamination and migration of the hypaxial precursors from the ventral lip to the limb buds through the transactivation of PAX3, MET and LBX1. Controls myoblast determination by transactivating MYF5, MYOD1 and MYF6. Controls somitic differentiation in myocyte through MYOG transactivation. Plays a role in synaptogenesis and sarcomere organization by participating in myofiber specialization during embryogenesis by activating fast muscle program in the primary myotome resulting in an up-regulation of fast muscle genes, including ATP2A1, MYL1 and TNNT3. Simultaneously, is also able to activate inhibitors of slow muscle genes, such as SOX6, HRASLS, and HDAC4, thereby restricting the activation of the slow muscle genes. During muscle regeneration, negatively regulates differentiation of muscle satellite cells through down-regulation of MYOG expression. During kidney development regulates the early stages of metanephros development and ureteric bud formation through regulation of GDNF, SALL1, PAX8 and PAX2 expression. Plays a role in gonad development by regulating both testis determination and size determination. In gonadal sex determination, transactivates ZFPM2 by binding a MEF3 consensus sequence, resulting in SRY up-regulation. In gonadal size determination, transactivates NR5A1 by binding a MEF3 consensus sequence resulting in gonadal precursor cell formation regulation. During olfactory development mediates the specification and patterning of olfactory placode through fibroblast growth factor and BMP4 signaling pathways and also regulates epithelial cell proliferation during placode formation. Promotes survival of sensory neurons during early trigeminal gangliogenesis. In the developing dorsal root ganglia, up-regulates SLC12A2 transcription. Regulates early thymus/parathyroid organogenesis through regulation of GCM2 and FOXN1 expression. Forms gustatory papillae during development of the tongue. Also plays a role during embryonic cranial skeleton morphogenesis. KEYWORDS: Acetylation;Cytoplasm;Developmental protein;DNA-binding;Homeobox;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus Cytoplasm " Q9UJ68,PROTEIN NAMES: Mitochondrial peptide methionine sulfoxide reductase (Peptide-methionine (S)-S-oxide reductase) (Peptide Met(O) reductase) (Protein-methionine-S-oxide reductase) (PMSR) PROTEIN FAMILY: MsrA Met sulfoxide reductase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the MsrA Met sulfoxide reductase family. FUNCTION: Has an important function as a repair enzyme for proteins that have been inactivated by oxidation. Catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine. KEYWORDS: Acetylation;Alternative initiation;Alternative promoter usage;Alternative splicing;Cytoplasm;Disulfide bond;Lipoprotein;Membrane;Mitochondrion;Myristate;Nucleus;Oxidoreductase;Redox-active center;Reference proteome;Transit peptide SUBCELLULAR LOCATION: [Isoform 1]: Mitochondrion.; SUBCELLULAR LOCATION: [Isoform 2]: Cytoplasm.; SUBCELLULAR LOCATION: [Isoform 3]: Cytoplasm. Nucleus.; SUBCELLULAR LOCATION: [Isoform 5]: Cytoplasm. Membrane ; Lipid-anchor MISCELLANEOUS: [Isoform 1]: Mitochondrial. Produced by alternative splicing.; MISCELLANEOUS: [Isoform 2]: Cytoplasmic. Produced by alternative promoter usage.; MISCELLANEOUS: [Isoform 3]: Cytoplasmic and nuclear. Produced by alternative promoter usage.; MISCELLANEOUS: [Isoform 4]: Produced by alternative splicing.; MISCELLANEOUS: [Isoform 5]: Cytoplasmic. Produced by alternative initiation. Q9UK05,PROTEIN NAMES: Growth/differentiation factor 2 (GDF-2) (Bone morphogenetic protein 9) (BMP-9) PROTEIN FAMILY: TGF-beta family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the TGF-beta family. FUNCTION: Potent circulating inhibitor of angiogenesis. Signals through the type I activin receptor ACVRL1 but not other Alks. Signaling through SMAD1 in endothelial cells requires TGF-beta coreceptor endoglin/ENG. KEYWORDS: 3D-structure;Angiogenesis;Cleavage on pair of basic residues;Cytokine;Disease variant;Disulfide bond;Glycoprotein;Growth factor;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted Q9UKG1,"PROTEIN NAMES: DCC-interacting protein 13-alpha (Dip13-alpha) (Adapter protein containing PH domain, PTB domain and leucine zipper motif 1) ORGANISM: Homo sapiens (Human) FUNCTION: Multifunctional adapter protein that binds to various membrane receptors, nuclear factors and signaling proteins to regulate many processes, such as cell proliferation, immune response, endosomal trafficking and cell metabolism. Regulates signaling pathway leading to cell proliferation through interaction with RAB5A and subunits of the NuRD/MeCP1 complex. Functions as a positive regulator of innate immune response via activation of AKT1 signaling pathway by forming a complex with APPL1 and PIK3R1 (By similarity). Inhibits Fc-gamma receptor-mediated phagocytosis through PI3K/Akt signaling in macrophages (By similarity). Regulates TLR4 signaling in activated macrophages (By similarity). Involved in trafficking of the TGFBR1 from the endosomes to the nucleus via microtubules in a TRAF6-dependent manner. Plays a role in cell metabolism by regulating adiponecting and insulin signaling pathways. Required for fibroblast migration through HGF cell signaling (By similarity). Positive regulator of beta-catenin/TCF-dependent transcription through direct interaction with RUVBL2/reptin resulting in the relief of RUVBL2-mediated repression of beta-catenin/TCF target genes by modulating the interactions within the beta-catenin-reptin-HDAC complex. KEYWORDS: 3D-structure;Cell cycle;Cell projection;Coiled coil;Cytoplasm;Cytoplasmic vesicle;Diabetes mellitus;Disease variant;Endosome;Membrane;Nucleus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Early endosome membrane ; Peripheral membrane protein Nucleus Cytoplasm Endosome Cell projection, ruffle Cytoplasmic vesicle, phagosome Note=Early endosomal membrane-bound and nuclear. Translocated into the nucleus upon release from endosomal membranes following internalization of EGF. " Q9UKN8,"PROTEIN NAMES: General transcription factor 3C polypeptide 4 (TF3C-delta) (Transcription factor IIIC 90 kDa subunit) (TFIIIC 90 kDa subunit) (TFIIIC90) (Transcription factor IIIC subunit delta) PROTEIN FAMILY: TFIIIC subunit 4 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the TFIIIC subunit 4 family. FUNCTION: Essential for RNA polymerase III to make a number of small nuclear and cytoplasmic RNAs, including 5S RNA, tRNA, and adenovirus-associated (VA) RNA of both cellular and viral origin. Has histone acetyltransferase activity (HAT) with unique specificity for free and nucleosomal H3. May cooperate with GTF3C5 in facilitating the recruitment of TFIIIB and RNA polymerase through direct interactions with BRF1, POLR3C and POLR3F. May be localized close to the A box. KEYWORDS: 3D-structure;Acetylation;Acyltransferase;Direct protein sequencing;DNA-binding;Isopeptide bond;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transferase;Ubl conjugation SUBCELLULAR LOCATION: Nucleus. " Q9UKT4,"PROTEIN NAMES: F-box only protein 5 (Early mitotic inhibitor 1) ORGANISM: Homo sapiens (Human) FUNCTION: Regulator of APC activity during mitotic and meiotic cell cycle. During mitotic cell cycle plays a role as both substrate and inhibitor of APC-FZR1 complex. During G1 phase, plays a role as substrate of APC-FZR1 complex E3 ligase. Then switches as an inhibitor of APC-FZR1 complex during S and G2 leading to cell-cycle commitment. As APC inhibitor, prevents the degradation of APC substrates at multiple levels: by interacting with APC and blocking access of APC substrates to the D-box coreceptor, formed by FZR1 and ANAPC10; by suppressing ubiquitin ligation and chain elongation by APC by preventing the UBE2C and UBE2S activities. Plays a role in genome integrity preservation by coordinating DNA replication with mitosis through APC inhibition in interphase to stabilize CCNA2 and GMNN in order to promote mitosis and prevent rereplication and DNA damage-induced cellular senescence. During oocyte maturation, plays a role in meiosis through inactivation of APC-FZR1 complex. Inhibits APC through RPS6KA2 interaction that increases FBXO5 affiniy for CDC20 leading to the metaphase arrest of the second meiotic division before fertilization (By similarity). Controls entry into the first meiotic division through inactivation of APC-FZR1 complex (By similarity). Promotes migration and osteogenic differentiation of mesenchymal stem cells. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: 3D-structure;Alternative splicing;Cell cycle;Cell division;Cytoplasm;Cytoskeleton;Metal-binding;Mitosis;Nucleus;Phosphoprotein;Reference proteome;Ubl conjugation;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Cytoplasm Cytoplasm, cytoskeleton, spindle Note=In interphase, localizes in a punctate manner in the nucleus and cytoplasm with some perinuclear concentration. In mitotic cells, localizes throughout the cell, particularly at the spindle. " Q9UKW6,PROTEIN NAMES: ETS-related transcription factor Elf-5 (E74-like factor 5) (Epithelium-restricted ESE-1-related Ets factor) (Epithelium-specific Ets transcription factor 2) (ESE-2) PROTEIN FAMILY: ETS family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the ETS family. FUNCTION: Transcriptionally activator that may play a role in regulating the later stages of keratinocytes terminal differentiation.; FUNCTION: Isoform 2 binds to DNA sequences containing the consensus nucleotide core sequence GGA[AT]. Transcriptionally activates SPRR2A and the parotid gland-specific PSP promoters. KEYWORDS: 3D-structure;Activator;Alternative splicing;DNA-binding;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus Q9UKZ1,"PROTEIN NAMES: CCR4-NOT transcription complex subunit 11 PROTEIN FAMILY: CNOT11 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the CNOT11 family. FUNCTION: Component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Is required for the association of CNOT10 with the CCR4-NOT complex. Seems not to be required for complex deadenylase function. KEYWORDS: 3D-structure;Cytoplasm;Methylation;Nucleus;Reference proteome;RNA-mediated gene silencing;Transcription;Transcription regulation;Translation regulation SUBCELLULAR LOCATION: Cytoplasm Nucleus " Q9UKZ9,PROTEIN NAMES: Procollagen C-endopeptidase enhancer 2 (Procollagen COOH-terminal proteinase enhancer 2) (PCPE-2) (Procollagen C-proteinase enhancer 2) ORGANISM: Homo sapiens (Human) FUNCTION: Binds to the C-terminal propeptide of types I and II procollagens and may enhance the cleavage of that propeptide by BMP1. KEYWORDS: Direct protein sequencing;Disulfide bond;Glycoprotein;Heparin-binding;Reference proteome;Repeat;Secreted;Signal SUBCELLULAR LOCATION: Secreted Q9UL33,"PROTEIN NAMES: Trafficking protein particle complex subunit 2-like protein PROTEIN FAMILY: TRAPP small subunits family, Sedlin subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the TRAPP small subunits family. Sedlin subfamily. FUNCTION: Plays a role in vesicular transport from endoplasmic reticulum to Golgi. KEYWORDS: Alternative splicing;Cytoplasm;Disease variant;Endoplasmic reticulum;Epilepsy;ER-Golgi transport;Golgi apparatus;Reference proteome;Transport SUBCELLULAR LOCATION: Cytoplasm, perinuclear region Endoplasmic reticulum Golgi apparatus " Q9ULA0,PROTEIN NAMES: Aspartyl aminopeptidase PROTEIN FAMILY: Peptidase M18 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the peptidase M18 family. FUNCTION: Aminopeptidase with specificity towards an acidic amino acid at the N-terminus. Likely to play an important role in intracellular protein and peptide metabolism. KEYWORDS: 3D-structure;Acetylation;Alternative initiation;Aminopeptidase;Cytoplasm;Hydrolase;Metal-binding;Metalloprotease;Phosphoprotein;Protease;Reference proteome;Zinc SUBCELLULAR LOCATION: Cytoplasm Q9ULD5,PROTEIN NAMES: Zinc finger protein 777 PROTEIN FAMILY: Krueppel C2H2-type zinc-finger protein family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein family. FUNCTION: May be involved in transcriptional repression. Inhibits cell proliferation through CDKN1A/p21 induction by down-regulation of NIBAN1/FAM129A at low cell density. KEYWORDS: DNA-binding;Isopeptide bond;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Repeat;Transcription;Transcription regulation;Ubl conjugation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Q9ULX6,"PROTEIN NAMES: A-kinase anchor protein 8-like (AKAP8-like protein) (Helicase A-binding protein 95) (HAP95) (Homologous to AKAP95 protein) (HA95) (Neighbor of A-kinase-anchoring protein 95) (Neighbor of AKAP95) PROTEIN FAMILY: AKAP95 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the AKAP95 family. FUNCTION: Could play a role in constitutive transport element (CTE)-mediated gene expression by association with DHX9. Increases CTE-dependent nuclear unspliced mRNA export. Proposed to target PRKACA to the nucleus but does not seem to be implicated in the binding of regulatory subunit II of PKA. May be involved in nuclear envelope breakdown and chromatin condensation. May be involved in anchoring nuclear membranes to chromatin in interphase and in releasing membranes from chromating at mitosis. May regulate the initiation phase of DNA replication when associated with TMPO isoform Beta. Required for cell cycle G2/M transition and histone deacetylation during mitosis. In mitotic cells recruits HDAC3 to the vicinity of chromatin leading to deacetylation and subsequent phosphorylation at 'Ser-10' of histone H3; in this function seems to act redundantly with AKAP8. May be involved in regulation of pre-mRNA splicing.; FUNCTION: (Microbial infection) In case of EBV infection, may target PRKACA to EBNA-LP-containing nuclear sites to modulate transcription from specific promoters.; FUNCTION: (Microbial infection) Can synergize with DHX9 to activate the CTE-mediated gene expression of type D retroviruses.; FUNCTION: (Microbial infection) In case of HIV-1 infection, involved in the DHX9-promoted annealing of host tRNA(Lys3) to viral genomic RNA as a primer in reverse transcription; in vitro negatively regulates DHX9 annealing activity. KEYWORDS: Acetylation;Alternative splicing;Cytoplasm;Host-virus interaction;Metal-binding;Methylation;mRNA processing;mRNA splicing;Nucleus;Phosphoprotein;Reference proteome;Repeat;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Nucleus matrix Nucleus speckle Nucleus, PML body Cytoplasm Note=Colocalizes with PRPF40A in the nuclear matrix. Nuclear at steady state but shuttles between the nucleus and cytoplasm. The shuttling property has been questioned. Colocalizes with EBNA-LP in PML bodies. " Q9UM11,"PROTEIN NAMES: Fizzy-related protein homolog (Fzr) (CDC20-like protein 1) (Cdh1/Hct1 homolog) (hCDH1) PROTEIN FAMILY: WD repeat CDC20/Fizzy family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the WD repeat CDC20/Fizzy family. FUNCTION: Substrate-specific adapter for the anaphase promoting complex/cyclosome (APC/C) E3 ubiquitin-protein ligase complex. Associates with the APC/C in late mitosis, in replacement of CDC20, and activates the APC/C during anaphase and telophase. The APC/C remains active in degrading substrates to ensure that positive regulators of the cell cycle do not accumulate prematurely. At the G1/S transition FZR1 is phosphorylated, leading to its dissociation from the APC/C. Following DNA damage, it is required for the G2 DNA damage checkpoint: its dephosphorylation and reassociation with the APC/C leads to the ubiquitination of PLK1, preventing entry into mitosis. Acts as an adapter for APC/C to target the DNA-end resection factor RBBP8/CtIP for ubiquitination and subsequent proteasomal degradation. Through the regulation of RBBP8/CtIP protein turnover, may play a role in DNA damage response, favoring DNA double-strand repair through error-prone non-homologous end joining (NHEJ) over error-free, RBBP8-mediated homologous recombination (HR). PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: 3D-structure;Acetylation;Alternative splicing;Cell cycle;Cell division;Cytoplasm;Disease variant;DNA damage;DNA repair;Epilepsy;Intellectual disability;Mitosis;Nucleus;Phosphoprotein;Reference proteome;Repeat;Ubl conjugation;Ubl conjugation pathway;WD repeat SUBCELLULAR LOCATION: [Isoform 2]: Nucleus.; SUBCELLULAR LOCATION: [Isoform 3]: Cytoplasm. MISCELLANEOUS: [Isoform 2]: Major.; MISCELLANEOUS: [Isoform 3]: Minor." Q9UM44,"PROTEIN NAMES: HERV-H LTR-associating protein 2 (Human endogenous retrovirus-H long terminal repeat-associating protein 2) ORGANISM: Homo sapiens (Human) FUNCTION: Through interaction with TMIGD2, costimulates T-cells in the context of TCR-mediated activation. Enhances T-cell proliferation and cytokine production via an AKT-dependent signaling cascade. KEYWORDS: Alternative splicing;Disulfide bond;Glycoprotein;Immunoglobulin domain;Membrane;Reference proteome;Repeat;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Single-pass type I membrane protein " Q9UMX1,"PROTEIN NAMES: Suppressor of fused homolog (SUFUH) PROTEIN FAMILY: SUFU family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the SUFU family. FUNCTION: Negative regulator in the hedgehog/smoothened signaling pathway. Down-regulates GLI1-mediated transactivation of target genes. Down-regulates GLI2-mediated transactivation of target genes. Part of a corepressor complex that acts on DNA-bound GLI1. May also act by linking GLI1 to BTRC and thereby targeting GLI1 to degradation by the proteasome. Sequesters GLI1, GLI2 and GLI3 in the cytoplasm, this effect is overcome by binding of STK36 to both SUFU and a GLI protein. Negative regulator of beta-catenin signaling (By similarity). Regulates the formation of either the repressor form (GLI3R) or the activator form (GLI3A) of the full-length form of GLI3 (GLI3FL). GLI3FL is complexed with SUFU in the cytoplasm and is maintained in a neutral state. Without the Hh signal, the SUFU-GLI3 complex is recruited to cilia, leading to the efficient processing of GLI3FL into GLI3R. When Hh signaling is initiated, SUFU dissociates from GLI3FL and the latter translocates to the nucleus, where it is phosphorylated, destabilized, and converted to a transcriptional activator (GLI3A). Required for normal embryonic development (By similarity). Required for the proper formation of hair follicles and the control of epidermal differentiation (By similarity). KEYWORDS: 3D-structure;Acetylation;Alternative splicing;Ciliopathy;Cytoplasm;Developmental protein;Disease variant;Isopeptide bond;Joubert syndrome;Nucleus;Phosphoprotein;Reference proteome;Tumor suppressor;Ubl conjugation SUBCELLULAR LOCATION: Cytoplasm Nucleus MISCELLANEOUS: [Isoform 1]: Major isoform." Q9UN81,"PROTEIN NAMES: LINE-1 retrotransposable element ORF1 protein (L1ORF1p) (LINE retrotransposable element 1) (LINE1 retrotransposable element 1) PROTEIN FAMILY: Transposase 22 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the transposase 22 family. FUNCTION: Nucleic acid-binding protein which is essential for retrotransposition of LINE-1 elements in the genome. Functions as a nucleic acid chaperone binding its own transcript and therefore preferentially mobilizing the transcript from which they are encoded. KEYWORDS: 3D-structure;Coiled coil;Cytoplasm;Nucleotide-binding;Nucleus;Reference proteome;Ubl conjugation SUBCELLULAR LOCATION: Nucleus, nucleolus Cytoplasm Cytoplasm, Cytoplasmic ribonucleoprotein granule Cytoplasm, Stress granule Note=Colocalizes with its encoding RNA in cytoplasmic ribonucleoprotein particle. Mainly cytoplasmic, rarely detected in the nucleus, possibly within the nucleolus. MISCELLANEOUS: Long interspersed element-1/LINE-1/L1 retrotransposons are present in more than 500'000 full (6 kb) or truncated copies in the human genome. Most of them are inactive but 80 to 100 of those elements could be transcribed, translated and active in any individual. An active LINE-1 encodes for 2 proteins translated from a single RNA containing 2 non-overlapping ORFs, ORF1 and ORF2. ORF1p is described in this entry as a representative of all ORF1p potentially expressed by active elements. ORF2p is described in the related entry AC O00370." Q9UNN5,PROTEIN NAMES: FAS-associated factor 1 (hFAF1) (UBX domain-containing protein 12) (UBX domain-containing protein 3A) ORGANISM: Homo sapiens (Human) FUNCTION: Ubiquitin-binding protein. Required for the progression of DNA replication forks by targeting DNA replication licensing factor CDT1 for degradation. Potentiates but cannot initiate FAS-induced apoptosis (By similarity). KEYWORDS: 3D-structure;Alternative splicing;Apoptosis;Nucleus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Nucleus Q9UNX4,"PROTEIN NAMES: WD repeat-containing protein 3 PROTEIN FAMILY: WD repeat WDR3/UTP12 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the WD repeat WDR3/UTP12 family. FUNCTION: Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome. KEYWORDS: 3D-structure;Isopeptide bond;Nucleus;Phosphoprotein;Reference proteome;Repeat;Ubl conjugation;WD repeat SUBCELLULAR LOCATION: Nucleus, nucleolus " Q9UNY5,PROTEIN NAMES: Zinc finger protein 232 (Zinc finger and SCAN domain-containing protein 11) PROTEIN FAMILY: Krueppel C2H2-type zinc-finger protein family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein family. FUNCTION: May be involved in transcriptional regulation. KEYWORDS: Alternative splicing;DNA-binding;Metal-binding;Nucleus;Reference proteome;Repeat;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus. Q9UP38,"PROTEIN NAMES: Frizzled-1 (Fz-1) (hFz1) (FzE1) PROTEIN FAMILY: G-protein coupled receptor Fz/Smo family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the G-protein coupled receptor Fz/Smo family. FUNCTION: Receptor for Wnt proteins. Activated by WNT3A, WNT3, WNT1 and to a lesser extent WNT2, but apparently not by WNT4, WNT5A, WNT5B, WNT6, WNT7A or WNT7B. Contradictory results showing activation by WNT7B have been described for mouse (By similarity). Functions in the canonical Wnt/beta-catenin signaling pathway. The canonical Wnt/beta-catenin signaling pathway leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues (Probable).; FUNCTION: (Microbial infection) Acts as a receptor for C.difficile toxin TcdB in the colonic epithelium. KEYWORDS: 3D-structure;Cell membrane;Developmental protein;Disulfide bond;G-protein coupled receptor;Glycoprotein;Membrane;Receptor;Reference proteome;Signal;Transducer;Transmembrane;Transmembrane helix;Ubl conjugation;Wnt signaling pathway SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein " Q9UQC2,"PROTEIN NAMES: GRB2-associated-binding protein 2 (GRB2-associated binder 2) (Growth factor receptor bound protein 2-associated protein 2) (pp100) PROTEIN FAMILY: GAB family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the GAB family. FUNCTION: Adapter protein which acts downstream of several membrane receptors including cytokine, antigen, hormone, cell matrix and growth factor receptors to regulate multiple signaling pathways. Regulates osteoclast differentiation mediating the TNFRSF11A/RANK signaling. In allergic response, it plays a role in mast cells activation and degranulation through PI-3-kinase regulation. Also involved in the regulation of cell proliferation and hematopoiesis. KEYWORDS: 3D-structure;Alternative splicing;Cell membrane;Cytoplasm;Membrane;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Cell membrane Membrane raft " Q9UQR0,"PROTEIN NAMES: Sex comb on midleg-like protein 2 PROTEIN FAMILY: SCM family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the SCM family. FUNCTION: Putative Polycomb group (PcG) protein. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development (By similarity). KEYWORDS: 3D-structure;Alternative splicing;Isopeptide bond;Nucleus;Phosphoprotein;Reference proteome;Repeat;Repressor;Transcription;Transcription regulation;Ubl conjugation SUBCELLULAR LOCATION: Nucleus " Q9UQY9,"PROTEIN NAMES: Cell cycle protein kinase spo4 (Sporulation-specific protein 4) PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family, CDC7 subfamily ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC7 subfamily. FUNCTION: Required for the initiation of meiosis II and progression through anaphase II. KEYWORDS: ATP-binding;Cell cycle;Cell division;Kinase;Meiosis;Nucleotide-binding;Nucleus;Phosphoprotein;Reference proteome;Serine/threonine-protein kinase;Sporulation;Transferase SUBCELLULAR LOCATION: Nucleus " Q9UR39,"PROTEIN NAMES: NAD-dependent protein deacetylase hst4 (Homologous to SIR2 protein 4) (Regulatory protein SIR2 homolog 4) PROTEIN FAMILY: Sirtuin family, Class I subfamily ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the sirtuin family. Class I subfamily. FUNCTION: NAD-dependent histone deacetylase, which contributes to both telomeric and centromeric silencing, proper cell cycle progression, DNA damage control, recombination, and genomic maintenance. KEYWORDS: Metal-binding;NAD;Nucleus;Reference proteome;Repressor;Transcription;Transcription regulation;Transferase;Zinc SUBCELLULAR LOCATION: Nucleus, nucleolus " Q9URZ4,PROTEIN NAMES: Cationic amino acid transporter 1 PROTEIN FAMILY: Amino acid-polyamine-organocation (APC) superfamily ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the amino acid-polyamine-organocation (APC) superfamily. FUNCTION: Major permease specifically involved in arginine and lysine uptake. KEYWORDS: Amino-acid transport;Cell membrane;Membrane;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Cell tip Note=enriched toward the growing ends of the cells. Q9US04,"PROTEIN NAMES: Glutathione hydrolase proenzyme 1 (Gamma-glutamyltransferase 1) (Gamma-glutamyltranspeptidase 1) [Cleaved into: Glutathione hydrolase 1 heavy chain; Glutathione hydrolase 1 light chain] PROTEIN FAMILY: Gamma-glutamyltransferase family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the gamma-glutamyltransferase family. FUNCTION: Catalyzes the transfer of the gamma-glutamyl moiety of glutathione (GSH) and other gamma-glutamyl compounds to amino acids and peptides. Major GSH-degrading enzyme, catalyzing the hydrolytic release of L-glutamate from GSH. PATHWAY: Sulfur metabolism; glutathione metabolism. KEYWORDS: Acyltransferase;Endoplasmic reticulum;Glycoprotein;Hydrolase;Membrane;Protease;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Single-pass type II membrane protein " Q9US26,"PROTEIN NAMES: Inner kinetochore subunit fta2 (CENP-P homolog) (Constitutive centromere-associated network protein fta2) (Sim4 complex subunit fta2) (Sim4-mal2-associated protein 2) PROTEIN FAMILY: CENP-P/CTF19 family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the CENP-P/CTF19 family. FUNCTION: Component of the kinetochore, a multiprotein complex that assembles on centromeric DNA and attaches chromosomes to spindle microtubules, mediating chromosome segregation and sister chromatid segregation during meiosis and mitosis. Component of the inner kinetochore COMA complex, which connects centromere-associated proteins and the outer kinetochore. COMA interacts with other inner kinetochore proteins to form the inner kinetochore constitutive centromere-associated network (CCAN), which serves as a structural platform for outer kinetochore assembly. Fta2, fta3 and fta4 associate with the central core (cnt) and inner repeat (inr) region of the centromere. KEYWORDS: Cell cycle;Cell division;Centromere;Chromosome;Chromosome partition;Kinetochore;Microtubule;Mitosis;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus. Chromosome, centromere, kinetochore. " Q9US60,"PROTEIN NAMES: Kinesin-like protein 3 (Kinesin-related protein 1) PROTEIN FAMILY: TRAFAC class myosin-kinesin ATPase superfamily, Kinesin family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. FUNCTION: Cytoplasmic motor that could play a role in Golgi membrane recycling. KEYWORDS: ATP-binding;Coiled coil;Cytoplasm;Cytoskeleton;Microtubule;Motor protein;Nucleotide-binding;Reference proteome SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton " Q9USK2,"PROTEIN NAMES: Histone-lysine N-methyltransferase set9 (Lysine N-methyltransferase 5) (SET domain protein 9) PROTEIN FAMILY: Class V-like SAM-binding methyltransferase superfamily, Histone-lysine methyltransferase family, Suvar4-20 subfamily ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. Suvar4-20 subfamily. FUNCTION: Histone methyltransferase that specifically trimethylates 'Lys-20' of histone H4 to form H4K20me3. H4 'Lys-20' methylation is apparently not involved in the regulation of gene expression or heterochromatin function but participates in DNA damage response by giving a 'histone mark' required for the recruitment of the checkpoint protein Crb2 to sites of DNA damage. KEYWORDS: Chromatin regulator;Chromosome;Methyltransferase;Nucleus;Reference proteome;S-adenosyl-L-methionine;Transferase SUBCELLULAR LOCATION: Nucleus Chromosome " Q9USM3,"PROTEIN NAMES: Chromatin structure-remodeling complex subunit sfh1 (RSC complex subunit sfh1) (SNF5 homolog 1) PROTEIN FAMILY: SNF5 family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the SNF5 family. FUNCTION: Component of the chromatin structure remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. Controls particularly membrane and organelle development genes. KEYWORDS: Chromatin regulator;Cytoplasm;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus Cytoplasm " Q9USP0,"PROTEIN NAMES: Translation initiation factor eIF2B subunit alpha (eIF2B GDP-GTP exchange factor subunit alpha) PROTEIN FAMILY: EIF-2B alpha/beta/delta subunits family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the eIF-2B alpha/beta/delta subunits family. FUNCTION: Acts as a component of the translation initiation factor 2B (eIF2B) complex, which catalyzes the exchange of GDP for GTP on the eukaryotic initiation factor 2 (eIF2) complex gamma subunit. Its guanine nucleotide exchange factor activity is repressed when bound to eIF2 complex phosphorylated on the alpha subunit, thereby limiting the amount of methionyl-initiator methionine tRNA available to the ribosome and consequently global translation is repressed. KEYWORDS: 3D-structure;Cytoplasm;Initiation factor;Protein biosynthesis;Reference proteome SUBCELLULAR LOCATION: Cytoplasm, cytosol " Q9USP2,PROTEIN NAMES: Golgi apyrase (ATP-diphosphatase) (ATP-diphosphohydrolase) (Adenosine diphosphatase) (ADPase) (Golgi nucleoside diphosphatase) PROTEIN FAMILY: GDA1/CD39 NTPase family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the GDA1/CD39 NTPase family. FUNCTION: Catalyzes the hydrolysis of phosphoanhydride bonds of nucleoside tri- and di-phosphates. Required for Golgi glycosylation and cell wall integrity. Involved in N-mannosylation of proteins in Golgi. PATHWAY: Protein modification; protein glycosylation. KEYWORDS: ATP-binding;Golgi apparatus;Hydrolase;Membrane;Nucleotide-binding;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus Membrane ; Single-pass membrane protein Q9USP8,"PROTEIN NAMES: Isocitrate dehydrogenase [NAD] subunit 2, mitochondrial (Isocitric dehydrogenase) (NAD(+)-specific ICDH) PROTEIN FAMILY: Isocitrate and isopropylmalate dehydrogenases family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the isocitrate and isopropylmalate dehydrogenases family. FUNCTION: Performs an essential role in the oxidative function of the citric acid cycle and is involved in glutamate biosynthesis. Also binds RNA; specifically to the 5'-untranslated leaders of mitochondrial mRNAs. KEYWORDS: Amino-acid biosynthesis;Magnesium;Manganese;Metal-binding;Mitochondrion;NAD;Oxidoreductase;Reference proteome;RNA-binding;Transit peptide;Tricarboxylic acid cycle SUBCELLULAR LOCATION: Mitochondrion " Q9USQ4,"PROTEIN NAMES: NASP-related protein sim3 (CENP-A escort protein sim3) (Silencing in the middle of the centromere protein 3) PROTEIN FAMILY: NASP family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the NASP family. FUNCTION: Histone H3 and H3-like CENP-A-specific chaperone. Promotes delivery and incorporation of CENP-A in centromeric chromatin, probably by escorting nascent CENP-A to CENP-A chromatin assembly factors. Required for central core silencing and normal chromosome segregation. KEYWORDS: Coiled coil;Nucleus;Reference proteome;Repeat;TPR repeat SUBCELLULAR LOCATION: Nucleus " Q9USR9,"PROTEIN NAMES: Inner kinetochore subunit cnp3 (CENP-C homolog) (Centromere protein 3) (Constitutive centromere-associated network protein cnp3) PROTEIN FAMILY: CENP-C/MIF2 family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the CENP-C/MIF2 family. FUNCTION: Component of the kinetochore, a multiprotein complex that assembles on centromeric DNA and attaches chromosomes to spindle microtubules, mediating chromosome segregation and sister chromatid segregation during meiosis and mitosis. Component of the inner kinetochore constitutive centromere-associated network (CCAN), which serves as a structural platform for outer kinetochore assembly. KEYWORDS: 3D-structure;DNA-binding;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus, nucleoplasm " Q9UT72,PROTEIN NAMES: E3 ubiquitin-protein ligase complex slx8-rfp subunit rfp2 (RING finger protein 2) (RING-type E3 ubiquitin transferase rfp2) ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) FUNCTION: Mediates ubiquitination and subsequent desumoylation/degradation of sumoylated proteins and proteins containing SUMO-like domains. Involved in maintaining genome stability where it acts in the cellular response to DNA damage. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Metal-binding;Nucleus;Reference proteome;Transferase;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Q9UTI7,PROTEIN NAMES: Probable thymidylate synthase (TS) (TSase) PROTEIN FAMILY: HFCD (homo-oligomeric flavin containing Cys decarboxylase) superfamily; Thymidylate synthase family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: In the N-terminal section; belongs to the HFCD (homo-oligomeric flavin containing Cys decarboxylase) superfamily.; SIMILARITY: In the C-terminal section; belongs to the thymidylate synthase family. FUNCTION: Required for both nuclear and mitochondrial DNA synthesis. PATHWAY: Pyrimidine metabolism; dTTP biosynthesis. KEYWORDS: Cytoplasm;Methyltransferase;Nucleotide biosynthesis;Reference proteome;Transferase SUBCELLULAR LOCATION: Cytoplasm Q9UTK7,PROTEIN NAMES: DSC E3 ubiquitin ligase complex subunit 2 (Defective for SREBP cleavage protein 2) (RING-type E3 ubiquitin transferase DSC2) (UBA domain-containing protein 14) ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) FUNCTION: Component of the DSC E3 ubiquitin ligase complex which is required for the sre1 transcriptional activator proteolytic cleavage to release the soluble transcription factor from the membrane in low oxygen or sterol conditions. The complex also plays an important role in the multivesicular body (MVB) pathway and functions in a post-endoplasmic reticulum pathway for protein degradation. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Golgi apparatus;Membrane;Phosphoprotein;Reference proteome;Transferase;Transmembrane;Transmembrane helix;Ubl conjugation pathway SUBCELLULAR LOCATION: Golgi apparatus membrane ; Multi-pass membrane protein Q9UTN3,"PROTEIN NAMES: Poly(A) RNA polymerase cid14 (PAP) (Caffeine-induced death protein 14) (Polynucleotide adenylyltransferase cid14) PROTEIN FAMILY: DNA polymerase type-B-like family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the DNA polymerase type-B-like family. FUNCTION: Required for 3' polyadenylation of the 5.8S and 25S rRNAs as a prelude to their degradation in the exosome. Involved in the nucleolar organization to ensure faithful chromosome segregation during mitosis. KEYWORDS: ATP-binding;Magnesium;Manganese;Metal-binding;mRNA processing;Nucleotide-binding;Nucleotidyltransferase;Nucleus;Reference proteome;RNA-binding;Transferase SUBCELLULAR LOCATION: Nucleus, nucleolus " Q9UTR6,"PROTEIN NAMES: Initiation-specific alpha-1,6-mannosyltransferase PROTEIN FAMILY: Glycosyltransferase 32 family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the glycosyltransferase 32 family. FUNCTION: Mannosyltransferase involved in outer chain elongation of asparagine-linked oligosaccharides of the type Man(9)GlcNAc(2). May otherwise add the first alpha-1,6-mannose to the Man(8)GlcNAc(2) core oligosaccharide from the ER. Represents the first enzymatic event required for synthesis of outer chain mannose linkages on yeast secretory proteins. KEYWORDS: Endoplasmic reticulum;Glycoprotein;Glycosyltransferase;Golgi apparatus;Membrane;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Single-pass type II membrane protein Golgi apparatus membrane ; Single-pass type II membrane protein Note=Is recycled between the trans-Golgi network and a late compartment of the endoplasmic reticulum. " Q9UTR8,"PROTEIN NAMES: NURS complex subunit red1 ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) FUNCTION: Promotes the exosome-mediated degradation of mRNAs containing a DSR (determinant of selective removal) signal sequence from mitotic cells. KEYWORDS: 3D-structure;Coiled coil;Metal-binding;Nucleus;Reference proteome;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Note=Localizes to distinct foci in the nucleus in mitotic cells, which disassemble during meiosis, although the protein is still present. " Q9UU96,"PROTEIN NAMES: Polynucleotide 5'-hydroxyl-kinase grc3 PROTEIN FAMILY: Clp1 family, NOL9/GRC3 subfamily ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the Clp1 family. NOL9/GRC3 subfamily. FUNCTION: Polynucleotide 5'-kinase required for both rRNA processing and heterochromatic gene silencing. KEYWORDS: ATP-binding;Chromosome;Kinase;Nucleotide-binding;Nucleus;Reference proteome;rRNA processing;Transcription;Transcription regulation;Transferase SUBCELLULAR LOCATION: Nucleus, nucleolus Nucleus Chromosome Note=Nuclear dots. " Q9UUA2,"PROTEIN NAMES: ATP-dependent DNA helicase pfh1 (DNA repair and recombination helicase pfh1) (PIF1 helicase homolog) (RRM3/PIF1 homolog 1) PROTEIN FAMILY: Helicase family, PIF1 subfamily ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the helicase family. PIF1 subfamily. FUNCTION: DNA-dependent ATPase and 5'-3' DNA helicase required for the maintenance of both mitochondrial and nuclear genome stability. Involved in the maintenance of mitochondrial (mtDNA). Required for both repair of mitochondrial DNA and recognition of a recombinogenic signal characterized by a 26-bp palindromic at sequence in the ery region of mitochondrial DNA. May have a general role in chromosomal replication by affecting Okazaki fragment maturation. Required for the completion of S-phase. KEYWORDS: Alternative initiation;ATP-binding;DNA damage;DNA recombination;DNA repair;DNA-binding;Helicase;Hydrolase;Mitochondrion;Nucleotide-binding;Nucleus;Phosphoprotein;Reference proteome;Transit peptide SUBCELLULAR LOCATION: [Isoform Nuclear]: Nucleus, nucleolus Note=Mainly concentrated in the nucleolus, and occasionally redistributes to single nuclear foci outside the nucleolus, probably sites of DNA repair.; SUBCELLULAR LOCATION: [Isoform Mitochondrial]: Mitochondrion MISCELLANEOUS: [Isoform Nuclear]: Produced by alternative initiation at Met-40 of isoform Mitochondrial." Q9UUG5,PROTEIN NAMES: Myosin regulatory light chain 1 ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) KEYWORDS: Calcium;Cytoplasm;Metal-binding;Motor protein;Myosin;Phosphoprotein;Reference proteome;Repeat SUBCELLULAR LOCATION: Cytoplasm. MISCELLANEOUS: This chain binds calcium. Q9UUI6,"PROTEIN NAMES: Replication termination factor 1 ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) FUNCTION: Mediates site-specific replication termination at the polar replication barrier RTS1, a barrier which ensures that replication of the mat1 locus in S.pombe occurs in the centromere-proximal direction. KEYWORDS: DNA replication;DNA replication inhibitor;DNA-binding;Nucleus;Reference proteome;Repeat SUBCELLULAR LOCATION: Nucleus " Q9UUJ6,PROTEIN NAMES: Nuclear elongation and deformation protein 1 PROTEIN FAMILY: Lipin family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the lipin family. FUNCTION: May have a role in the maintenance of the nuclear envelope structure and in minichromosome stability. KEYWORDS: Phosphoprotein;Reference proteome Q9UUL2,PROTEIN NAMES: DNA repair protein rhp57 (RAD57 homolog) PROTEIN FAMILY: RecA family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the RecA family. FUNCTION: Involved in recombination DNA repair and in the repair of gamma-ray-induced damage. KEYWORDS: ATP-binding;DNA damage;DNA repair;Nucleotide-binding;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus Q9UVC3,"PROTEIN NAMES: Lanosterol 14-alpha demethylase (CYPLI) (Cytochrome P450 51) (Cytochrome P450-14DM) (Cytochrome P450-LIA1) (Sterol 14-alpha demethylase) PROTEIN FAMILY: Cytochrome P450 family ORGANISM: Cunninghamella elegans SIMILARITY: Belongs to the cytochrome P450 family. FUNCTION: Sterol 14alpha-demethylase that plays a critical role in the third module of ergosterol biosynthesis pathway, being ergosterol the major sterol component in fungal membranes that participates in a variety of functions (By similarity). The third module or late pathway involves the ergosterol synthesis itself through consecutive reactions that mainly occur in the endoplasmic reticulum (ER) membrane (By similarity). In filamentous fungi, during the initial step of this module, lanosterol (lanosta-8,24-dien-3beta-ol) can be metabolized to eburicol (By similarity). Sterol 14alpha-demethylase catalyzes the three-step oxidative removal of the 14alpha-methyl group (C-32) of both these sterols in the form of formate, and converts eburicol and lanosterol to 14-demethyleburicol (4,4,24-trimethylergosta-8,14,24(28)-trienol) and 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol, respectively, which are further metabolized by other enzymes in the pathway to ergosterol (By similarity). Can also use substrates not intrinsic to fungi, such as 24,25-dihydrolanosterol (DHL), producing 4,4-dimethyl-8,14-cholestadien-3-beta-ol, but at lower rates than the endogenous substrates (By similarity). PATHWAY: Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 1/6. KEYWORDS: Heme;Iron;Lipid biosynthesis;Lipid metabolism;Membrane;Metal-binding;Monooxygenase;Oxidoreductase;Steroid biosynthesis;Steroid metabolism;Sterol biosynthesis;Sterol metabolism SUBCELLULAR LOCATION: Membrane " Q9UWR6,"PROTEIN NAMES: Geranylfarnesyl diphosphate synthase (GFPS) (Farnesylgeranyl diphosphate synthase) (FGPP synthase) PROTEIN FAMILY: FPP/GGPP synthase family ORGANISM: Aeropyrum pernix SIMILARITY: Belongs to the FPP/GGPP synthase family. FUNCTION: Probably involved in biosynthesis of the precursor for C25 (sesterterpanyl chain) moiety of C25-C25 diether (2,3-di-O-sesterterpanyl-sn-glycero) membrane lipid. Catalyzes the condensation of isopentenyl pyrophosphate with the allylic pyrophosphates to yield all-trans geranylfarnesyl diphosphate (GFPP). Geranylgeranyl diphosphate (GGPP) is the preferred substrate, however methylallyl diphosphate (DMAPP), farnesyl diphosphate (FPP) and geranyl diphosphate (GPP) can also be used as allylic substrate. KEYWORDS: Lipid biosynthesis;Lipid metabolism;Magnesium;Metal-binding;Transferase " Q9V076,"PROTEIN NAMES: Ribonuclease J (RNase J) (Pab-RNase J) PROTEIN FAMILY: Metallo-beta-lactamase superfamily, RNA-metabolizing metallo-beta-lactamase-like family, Archaeal RNase J subfamily ORGANISM: Pyrococcus abyssi (strain GE5 / Orsay) SIMILARITY: Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. Archaeal RNase J subfamily. FUNCTION: A highly processive 5'-3' exoribonuclease; no evidence has been seen for endonuclease activity. Prefers 5'-phosphate or 5'-hydroxyl ends; 5'-triphosphate substrates are very poorly degraded, does not degrade circular RNA. Does not degrade pre-tRNA(Trp) suggesting it is inhibited by strong secondary structures. Also degrades ssNDA but not dsDNA. KEYWORDS: Cytoplasm;Exonuclease;Hydrolase;Metal-binding;Nuclease;RNA-binding;Zinc SUBCELLULAR LOCATION: Cytoplasm MISCELLANEOUS: Mutagenesis of His-410 to Val in the T.kodakaraensis homolog (AC Q5JH57) reduces exoribonuclease activity to 1%." Q9V345,"PROTEIN NAMES: COP9 signalosome complex subunit 4 (Dch4) (Signalosome subunit 4) PROTEIN FAMILY: CSN4 family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the CSN4 family. FUNCTION: Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of the SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF. The CSN complex plays an essential role in oogenesis and embryogenesis and is required for proper photoreceptor R cell differentiation and promote lamina glial cell migration or axon targeting. It also promotes Ubl-dependent degradation of cyclin E (CycE) during early oogenesis. KEYWORDS: Cytoplasm;Developmental protein;Differentiation;Nucleus;Oogenesis;Reference proteome;Signalosome SUBCELLULAR LOCATION: Cytoplasm Nucleus " Q9V3C1,"PROTEIN NAMES: Protein Vhl PROTEIN FAMILY: VHL family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the VHL family. FUNCTION: Involved in development of tracheal vasculature. Probably involved in halting cell migration at the end of vascular tube outgrowth. Possesses E3 ubiquitin ligase activity when in complex with Elongin BC complex, Cul2 and Rox1a/Rbx1, and can target sima/Hif1a for ubiquitination. May play a critical role in promoting microtubule stabilization when tubulins are correctly folded by the prefoldin complex. If tubulin is incorrectly folded, may promote its degradation. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Angiogenesis;Developmental protein;Differentiation;Reference proteome;Ubl conjugation pathway " Q9V3I8,"PROTEIN NAMES: N-glycosylase/DNA lyase (dOgg1) [Includes: 8-oxoguanine DNA glycosylase ; DNA-(apurinic or apyrimidinic site) lyase (AP lyase) ] PROTEIN FAMILY: Type-1 OGG1 family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the type-1 OGG1 family. FUNCTION: DNA repair enzyme that incises DNA at 8-oxoG residues. Excises 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine (FAPY) from damaged DNA. Has a beta-lyase activity that nicks DNA 3' to the lesion. Efficiently incises DNA duplexes containing 8-hydroxyguanine (8-OH-Gua), 8-hydroxyadenine (8-OH-Ade) and abasic (AP) sites placed opposite to a cytosine. KEYWORDS: Cytoplasm;DNA damage;DNA repair;Glycosidase;Hydrolase;Lyase;Multifunctional enzyme;Nuclease;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus Cytoplasm Note=Nuclear and cytoplasmic in nurse cells and oocyte. " Q9V3N1,"PROTEIN NAMES: Serine protease inhibitor 27A (Serpin 27A) PROTEIN FAMILY: Serpin family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the serpin family. FUNCTION: Serine protease inhibitor that functions in embryonic dorsoventral patterning and the melanization immune response. Regulates dorsoventral axis formation during early development by inhibiting the serine protease easter, and is therefore important for restricting activity of the Toll signaling pathway to the ventral part of the embryo. Also plays an essential role in the melanization immune response to both physical wounding and septic infection using certain bacteria and fungi. Negatively regulates the Hayan-dependent prophenoloxidase 1 (PPO1)-activating cascade in the hemolymph by inhibiting the serine proteases MP1 and Sp7. May be involved in negatively regulating the melanotic encapsulation around eggs of the parasite L. boulardi. KEYWORDS: Glycoprotein;Protease inhibitor;Reference proteome;Secreted;Serine protease inhibitor;Signal SUBCELLULAR LOCATION: Secreted Note=Secreted into the hemolymph. Detected in the perivitelline fluid of embryos. " Q9V3Q6,"PROTEIN NAMES: Mitogen-activated protein kinase kinase kinase 7 (TGF-beta-activated kinase 1) (dTAK1) PROTEIN FAMILY: Protein kinase superfamily, STE Ser/Thr protein kinase family, MAP kinase kinase kinase subfamily ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily. FUNCTION: Component of a protein kinase signal transduction cascade. Mediator of TGF-beta signal transduction. Responsible for activation of the JNK MAPK pathway (basket, bsk and hemipterous, hep) in response to LPS. Component of the NF-kappa-B pathway; relish-mediated JNK inhibition involves proteasomal degradation of Tak1; certain targets of Relish that are induced during immune responses may facilitate destruction of Tak1 and switch off the JNK cascade. Participates in diverse roles such as control of cell shape and regulation of apoptosis. KEYWORDS: ATP-binding;Immunity;Innate immunity;Kinase;Magnesium;Metal-binding;Nucleotide-binding;Reference proteome;Serine/threonine-protein kinase;Transferase " Q9V3R8,"PROTEIN NAMES: UbiA prenyltransferase domain-containing protein 1 homolog (Protein heixuedian) PROTEIN FAMILY: UbiA prenyltransferase family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the UbiA prenyltransferase family. FUNCTION: Prenyltransferase that mediates the formation of menaquinone-4 (MK-4), a vitamin K2 isoform, thereby acting as a mitochondrial electron carrier. Mediates the conversion of phylloquinone (PK) into MK-4, probably by cleaving the side chain of phylloquinone (PK) to release 2-methyl-1,4-naphthoquinone (menadione; K3) and then prenylating it with geranylgeranyl pyrophosphate (GGPP) to form MK-4. MK-4 acts as a membrane electron carrier downstream of a electron transport chain complex, improving mitochondrial oxygen consumption. PATHWAY: Quinol/quinone metabolism; menaquinone biosynthesis. KEYWORDS: Membrane;Menaquinone biosynthesis;Mitochondrion;Prenyltransferase;Reference proteome;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Mitochondrion membrane ; Multi-pass membrane protein " Q9V3T9,"PROTEIN NAMES: NADPH:adrenodoxin oxidoreductase, mitochondrial (AR) (Adrenodoxin reductase) (Ferredoxin--NADP(+) reductase) (Ferredoxin reductase) PROTEIN FAMILY: Ferredoxin--NADP reductase type 1 family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the ferredoxin--NADP reductase type 1 family. FUNCTION: Required for synthesis of steroid hormones, for olfactory sensory behavior and completion of the second larval molt (a steroid mediated developmental transition) and pupariation. PATHWAY: Steroid metabolism; cholesterol metabolism. KEYWORDS: Electron transport;FAD;Flavoprotein;Membrane;Mitochondrion;Mitochondrion inner membrane;NADP;Oxidoreductase;Reference proteome;Transit peptide;Transport SUBCELLULAR LOCATION: Mitochondrion inner membrane ; Peripheral membrane protein " Q9V3U0,"PROTEIN NAMES: Long-chain-fatty-acid--CoA ligase heimdall (Very long-chain-fatty-acid--CoA ligase double bubble) PROTEIN FAMILY: ATP-dependent AMP-binding enzyme family, Bubblegum subfamily ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the ATP-dependent AMP-binding enzyme family. Bubblegum subfamily. FUNCTION: Mediates activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Probably by regulating lipid storage and catabolism, plays a role in neuronal function. KEYWORDS: ATP-binding;Fatty acid metabolism;Ligase;Lipid metabolism;Nucleotide-binding;Reference proteome MISCELLANEOUS: In Norse mythology, Heimdall is the fictional character that for ages guarded the bridge to Asgard without sleeping and without the consequences of sleep deprivation." Q9V3Z1,"PROTEIN NAMES: Tribbles PROTEIN FAMILY: Protein kinase superfamily, CAMK Ser/Thr protein kinase family, Tribbles subfamily ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. Tribbles subfamily. FUNCTION: Adapter protein that negatively regulates different signaling pathways to coordinate cell differentiation, proliferation, migration and growth. Functions by binding to key regulatory proteins and either blocks their activity or regulates their turnover by the proteasome. In various developing tissues functions as a cell cycle regulator that mediates cell proliferation according to the requirements of the developmental program. Acts by inducing the proteasomal degradation of the CD25 mitotic activators stg and twe at critical stages of development to delay entry into mitosis and thus mediate cell proliferation. During gastrulation, negatively regulates stg to delay mitosis in the ventral region of the embryonic mesoderm thus allowing invagination to be completed before cell division takes place. Delaying stg-dependent mitosis during bristle development and in migrating germline pole cells also arrests their cell divisions, whereas in cystocytes it promotes their cell divisions. Involved in the regulation of the mid-blastula transition; promotes the destruction of twe resulting in the cell cycle arrest in G2 of cycle 14 which delays mitosis and thus reduces cell proliferation allowing cell fate specification and morphogenesis to take place. In germline cells, blocks border cell migration during oogenesis by binding to slbo/C/EBP and promoting its ubiquitination and degradation by the proteasome. May function in a negative feedback loop with slbo to coordinate proper border cell migration. During tissue growth negatively regulates insulin signaling by binding to Akt1 and blocking its phosphorylation-dependent activation. However it may also function downstream in the insulin signaling pathway, acting with Akt1 to direct foxo degradation. Essential for the proper formation of operant place and aversive olfactory memories. KEYWORDS: Cell cycle;Cytoplasm;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus Cytoplasm Cytoplasm, cell cortex Note=Weakly cytoplasmic. In the main body follicle cells, strong nuclear accumulation at stage 10 that decreases to low levels in the cytoplasm by stage 12. In border cells, high levels of expression detected prior to border cell (BC) delamination (from stages 7 to 8). At stage 9, expression levels remains high in BC as their migration begins but decreases throughout migration. By stage 10 levels are low in BC nuclei when they arrive at the nurse cell/oocyte boundary. MISCELLANEOUS: 'tribbles' is named after fictional small round organisms from the Star Trek universe that proliferate uncontrollably." Q9V3Z2,"PROTEIN NAMES: Serine protease 7 (Melanization protease 2) PROTEIN FAMILY: Peptidase S1 family, CLIP subfamily ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the peptidase S1 family. CLIP subfamily. FUNCTION: Serine protease which, by cleaving and activating prophenoloxidase (PPO1) after immune challenge, plays an essential role in the melanization immune response to septic wounding. May function in diverse Hayan-dependent PPO1-activating cascades that are negatively controlled by different serpin proteins; Spn27A in the hemolymph and Spn77BA in the trachea. Important for the innate immune response to fungi. Regulation of melanization and PPO1 activation appears to be largely independent of the Toll signaling pathway. KEYWORDS: Calcium;Disulfide bond;Glycoprotein;Hydrolase;Metal-binding;Protease;Reference proteome;Secreted;Serine protease;Signal;Zymogen SUBCELLULAR LOCATION: Secreted " Q9V407,PROTEIN NAMES: Axin (Axis inhibition protein) (d-Axin) (dAxin) ORGANISM: Drosophila melanogaster (Fruit fly) FUNCTION: Inhibitor of the WG signaling pathway. Down-regulates beta-catenin (armadillo=ARM). Probably facilitate the phosphorylation of beta-catenin and APC by GSK3B (zeste-white 3=ZW3). KEYWORDS: Cytoplasm;Developmental protein;Phosphoprotein;Reference proteome;Wnt signaling pathway SUBCELLULAR LOCATION: Cytoplasm Q9V4S8,"PROTEIN NAMES: COP9 signalosome complex subunit 7 (Dch7) (Signalosome subunit 7) PROTEIN FAMILY: CSN7/EIF3M family, CSN7 subfamily ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the CSN7/EIF3M family. CSN7 subfamily. FUNCTION: Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of the SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF. The CSN complex plays an essential role in oogenesis and embryogenesis and is required for proper photoreceptor R cell differentiation and promote lamina glial cell migration or axon targeting. It also promotes Ubl-dependent degradation of cyclin E (CycE) during early oogenesis. KEYWORDS: Cytoplasm;Developmental protein;Differentiation;Nucleus;Oogenesis;Phosphoprotein;Reference proteome;Signalosome SUBCELLULAR LOCATION: Cytoplasm Nucleus " Q9V4Z9,"PROTEIN NAMES: Protein lines PROTEIN FAMILY: Protein lines family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the protein lines family. FUNCTION: Has a dual role as a segment polarity protein and as a modulator of the Abd-B protein. Required for Abd-B to activate the transcription of genes (including ems, cut and sal) that are involved in posterior spiracle morphogenesis. Also required for Abd-B to form an eighth abdominal denticle belt. Acts in a hierarchy downstream of drm and upstream of bowl during foregut and hindgut patterning and morphogenesis. Involved in cell rearrangement during elongation of the embryonic hindgut. Required to regulate expression of embryonic hindgut patterning genes in order to establish the large intestine and at least some rectum, and to repress small intestine fate. Required for late wingless (wg)-dependent cell fate specification in the dorsal embryonic epidermis. Acts in concert with wg to regulate expression of wg itself and also to regulate wg-target genes. May have a role in ventral epidermal patterning, independent of wg signaling. KEYWORDS: Activator;Cytoplasm;Developmental protein;Nucleus;Reference proteome;Repressor;Segmentation polarity protein;Transcription;Transcription regulation;Wnt signaling pathway SUBCELLULAR LOCATION: Cytoplasm Nucleus Note=Cytoplasmic in the half of the parasegment, posterior to the engrailed (en) domain, and nuclear in the half of the parasegment anterior to the en domain. " Q9V576,"PROTEIN NAMES: Putative phosphatidate phosphatase (Germ cell guidance factor) (Phosphatidic acid phosphatase type 2) (Protein wunen) PROTEIN FAMILY: PA-phosphatase related phosphoesterase family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the PA-phosphatase related phosphoesterase family. FUNCTION: Responsible for guiding the germ cells early in the process of migration from the lumen of the developing gut towards the overlying mesoderm, where the germ cells enter the gonads. May be involved in lipid metabolism. KEYWORDS: Alternative splicing;Developmental protein;Glycoprotein;Hydrolase;Membrane;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Multi-pass membrane protein " Q9V629,"PROTEIN NAMES: Protein cutoff PROTEIN FAMILY: DXO/Dom3Z family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the DXO/Dom3Z family. FUNCTION: Involved in the piRNA pathway in germline tissues. Part of the Rhino-Deadlock-Cutoff (RDC) complex that stimulates piRNA biogenesis from chromatin regions corresponding to dual-strand, but not single-stranded, piRNA clusters. Promotes transcription of long piRNA precursors by preventing termination at canonical poly(A) sites. As part of the RDC complex, is recruited to chromatin enriched in histone modification H3K9me3 and might contribute to complex interaction by binding nascent transcript nucleic acid chains. Associates with chromatin upon exposure to homologous piRNA. Suppresses cleavage at canonical poly(A) sites by blocking recruitment of the cleavage and polyadenylation specificity factor (CPSF) complex and prevents transcriptional termination by RNA polymerase II, facilitating transcriptional read-through. As part of the RDC complex, involved in suppression of splicing. Catalytically inactive, lacking 5'-3' exonuclease and pyrophosphohydrolase activities. Stabilizes uncapped piRNA precursors in the nucleus, probably by sequestering or blocking the exonuclease activity of Rat1. May also be involved in siRNA biogenesis from dual-strand piRNA clusters. KEYWORDS: Chromosome;Cytoplasm;Nucleus;Reference proteome SUBCELLULAR LOCATION: Cytoplasm. Nucleus Chromosome Note=Localized to perinuclear puncta in the nurse cells of younger egg chambers. Accumulates at centromeric/pericentromeric positions in germ-cell nuclei and strongly colocalizes with the major heterochromatic domains. Localizes to nuclear foci corresponding to dual-strand, but not single-strand, piRNA clusters. All components of the Rhino-Deadlock-Cutoff (RDC) Complex associate with these nuclear foci and are required for their formation. " Q9V6G5,"PROTEIN NAMES: Tafazzin (TAZ) (dTAZ) PROTEIN FAMILY: Taffazin family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the taffazin family. FUNCTION: Acyltransferase required to remodel newly synthesized phospholipid cardiolipin (1',3'-bis-[1,2-diacyl-sn-glycero-3-phospho]-glycerol or CL), a key component of the mitochondrial inner membrane, with tissue specific acyl chains necessary for adequate mitochondrial function. Its role in cellular physiology is to improve mitochondrial performance (By similarity). CL is critical for the coassembly of lipids and proteins in mitochondrial membranes. For instance, remodeling of the acyl groups of CL in the mitochondrial inner membrane affects the assembly and stability of respiratory chain complex IV and its supercomplex forms (By similarity). Catalyzes the transacylation between phospholipids and lysophospholipids, with the highest rate being between phosphatidylcholine (1,2-diacyl-sn-glycero-3-phosphocholine or PC) and CL. Catalyzes both 1-acyl-sn-glycero-3-phosphocholine (lysophosphatidylcholine or LPC) reacylation and PC-CL transacylation, that means, it exchanges acyl groups between CL and PC by a combination of forward and reverse transacylations. Also catalyzes transacylations between other phospholipids such as phosphatidylethanolamine (1,2-diacyl-sn-glycero-3-phosphoethanolamine or PE) and CL, between PC and PE, and between PC and phosphatidate (1,2-diacyl-sn-glycero-3-phosphate or PA), although at lower rate. Not regiospecific, it transfers acyl groups into any of the sn-1 and sn-2 positions of the monolysocardiolipin (MLCL), which is an important prerequisite for uniformity and symmetry in CL acyl distribution. Cannot transacylate dilysocardiolipin (DLCL), thus, the role of MLCL is limited to that of an acyl acceptor. CoA-independent, it can reshuffle molecular species within a single phospholipid class. Redistributes fatty acids between MLCL, CL, and other lipids, which prolongs the half-life of CL. Its action is completely reversible, which allows for cyclic changes, such as fission and fusion or bending and flattening of the membrane. Hence, by contributing to the flexibility of the lipid composition, it plays an important role in the dynamics of mitochondria membranes. Essential for the final stage of spermatogenesis, spermatid individualization. Required for the initiation of mitophagy (By similarity). PATHWAY: Phospholipid metabolism. KEYWORDS: Acyltransferase;Alternative splicing;Endoplasmic reticulum;Golgi apparatus;Lipid metabolism;Membrane;Mitochondrion;Mitochondrion inner membrane;Mitochondrion outer membrane;Phospholipid metabolism;Reference proteome;Transferase SUBCELLULAR LOCATION: Mitochondrion outer membrane ; Peripheral membrane protein ; Intermembrane side Mitochondrion inner membrane ; Peripheral membrane protein ; Intermembrane side Mitochondrion.; SUBCELLULAR LOCATION: [Isoform A]: Mitochondrion membrane.; SUBCELLULAR LOCATION: [Isoform B]: Mitochondrion membrane Golgi apparatus membrane Endoplasmic reticulum membrane.; SUBCELLULAR LOCATION: [Isoform C]: Mitochondrion membrane MISCELLANEOUS: The enzyme was named after a masochistic character Tafazzi, once popular on Italian television, apparently due to the difficulty encountered for its identification and characterization." Q9V730,"PROTEIN NAMES: Exostosin-1 (Protein tout-velu) (TTV) PROTEIN FAMILY: Glycosyltransferase 47 family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the glycosyltransferase 47 family. FUNCTION: Glycosyltransferase required for the biosynthesis of heparan-sulfate and responsible for the alternating addition of beta-1-4-linked glucuronic acid (GlcA) and alpha-1-4-linked N-acetylglucosamine (GlcNAc) units to nascent heparan sulfate chains. Botv is the trigger of heparan sulfate chain initiation and polymerization takes place by a complex of ttv and sotv. Plays a central role in the diffusion of morphogens hedgehog (hh), wingless (wg) and decapentaplegic (dpp) via its role in heparan sulfate proteoglycans (HSPGs) biosynthesis which are required for movement of hh, dpp and wg morphogens. PATHWAY: Protein modification; protein glycosylation.; PATHWAY: Glycan metabolism; heparan sulfate biosynthesis.; PATHWAY: Glycan metabolism; heparin biosynthesis. KEYWORDS: Developmental protein;Disulfide bond;Endoplasmic reticulum;Glycoprotein;Glycosyltransferase;Golgi apparatus;Manganese;Membrane;Metal-binding;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix;Wnt signaling pathway SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Single-pass type II membrane protein. Golgi apparatus membrane; Single-pass type II membrane protein. Note=Localization to the Golgi may be regulated by sau. MISCELLANEOUS: 'Tout velu' means 'very hairy' in French." Q9V785,PROTEIN NAMES: SH3 domain-binding protein 5 homolog (Protein parcase) PROTEIN FAMILY: SH3BP5 family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the SH3BP5 family. KEYWORDS: Coiled coil;Phosphoprotein;Reference proteome;SH3-binding Q9V7P1,"PROTEIN NAMES: U3 small nucleolar RNA-associated protein 18 homolog (Protein wicked) (WD repeat protein l(2)k07824) PROTEIN FAMILY: WD repeat UTP18 family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the WD repeat UTP18 family. FUNCTION: Component of a nucleolar small nuclear ribonucleoprotein particle (snoRNP) thought to participate in the processing and modification of pre-ribosomal RNA. Regulation of cell size by ribosome synthesis is an important parameter for stem cell maintenance and function. KEYWORDS: Developmental protein;Nucleus;Phosphoprotein;Reference proteome;Repeat;rRNA processing;WD repeat SUBCELLULAR LOCATION: Nucleus, nucleolus Note=Asymmetric segregation of expression upon mitosis both in germline stem cells (GSCs) and dividing larval neural stem cells (NSCs) (at protein level). " Q9V7Y2,"PROTEIN NAMES: Sphingosine-1-phosphate lyase (S1PL) (SP-lyase) (SPL) (Sphingosine-1-phosphate aldolase) PROTEIN FAMILY: Group II decarboxylase family, Sphingosine-1-phosphate lyase subfamily ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the group II decarboxylase family. Sphingosine-1-phosphate lyase subfamily. FUNCTION: Cleaves phosphorylated sphingoid bases (PSBs), such as sphingosine-1-phosphate, into fatty aldehydes and phosphoethanolamine. Sphingolipid catabolism is required for normal development including viability, reproduction and muscle development. PATHWAY: Lipid metabolism; sphingolipid metabolism. KEYWORDS: Developmental protein;Endoplasmic reticulum;Lipid metabolism;Lyase;Membrane;Pyridoxal phosphate;Reference proteome;Signal-anchor;Sphingolipid metabolism;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Single-pass type III membrane protein " Q9V9V9,"PROTEIN NAMES: Alpha/beta-tubulin-N-acetyltransferase 9 (Microtubule-associated Nat9) PROTEIN FAMILY: Acetyltransferase family, GNAT subfamily ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the acetyltransferase family. GNAT subfamily. FUNCTION: N-acetyltransferase that mediates the acetylation of the N-terminal residues of alpha- and beta-tubulin. Required for microtubule stability and inhibition of JNK signaling to promote cell survival during development, possibly acting independently of its N-acetyltransferase activity. Necessary for the stabilization of spindle microtubules and for mitosis progression. Regulates microtubule stability by inhibiting Spastin-mediated depolymerization and promoting Eb1-mediated polymerization. KEYWORDS: Acyltransferase;Cytoplasm;Cytoskeleton;Nucleus;Reference proteome;Transferase SUBCELLULAR LOCATION: Nucleus Cytoplasm, cytoskeleton, spindle Cytoplasm, cytoskeleton, spindle pole Note=In syncytial embryos, localization varies during mitosis. During interphase and prophase, detected around the nucleus including surrounding tubulins. During metaphase and anaphase, highly enriched at the spindle poles and around the spindle microtubules. During telophase, detected in the midbody region containing spindle microtubules. " Q9V9Y4,"PROTEIN NAMES: pH-sensitive chloride channel 2 (Ligand-gated chloride channel protein hodor) PROTEIN FAMILY: Ligand-gated ion channel family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the ligand-gated ion channel (TC 1.A.9) family. FUNCTION: Ligand and pH-gated channel that mediates chloride transport primarily in the mid-gut and thereby functions in larval metabolism and fluid homeostasis. Channel opening is triggered by zinc binding or, to a lesser extent, an increase in extracellular pH. Zinc-dependent activity in the mid-gut is required for modulating Tor-dependent metabolic programs that promote larval feeding and systematic growth. It may therefore act as an intestinal zinc sensor that mediates larval growth and metabolism in response to micronutrient availability. Activates Tor signaling via its activity in maintaining lysosome homeostasis in interstitial cells and/or by its role in activating the release of insulin-like peptides in the brain after feeding, via an unknown mechanism. Functions in lysosome homeostasis by regulating chloride transport into enterocyte lysosomes to sustain V-ATPase function which maintains lysosomal acidification and consequently promotes Tor activation at the lysosome membrane. Also appears to play a role in regulating fluid secretion and osmotic homeostasis in Malpighian tubules in response to the pH of extracellular urine. This function is important for proper urine production during diuresis. KEYWORDS: Cell membrane;Cell projection;Chloride;Chloride channel;Endosome;Glycoprotein;Ion channel;Ion transport;Ligand-gated ion channel;Lysosome;Membrane;Reference proteome;Signal;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Apical cell membrane ; Multi-pass membrane protein Cell projection, microvillus membrane ; Multi-pass membrane protein Late endosome membrane ; Multi-pass membrane protein Lysosome membrane ; Multi-pass membrane protein Note=Enriched on the apical side of gut interstitial cells; on the brush-border and on the lysosomes. MISCELLANEOUS: The name 'hodor' is an acronym for 'hold on, don't rush', referring to the developmental delay phenotype in mutants." Q9VA00,"PROTEIN NAMES: Protein zwilch PROTEIN FAMILY: ZWILCH family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the ZWILCH family. FUNCTION: Essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. Required for the assembly of the dynein-dynactin, Mad2 complexes and spindly/CG15415 onto kinetochores. Its function related to the spindle assembly machinery is proposed to depend on its association in the RZZ complex. Failure to assemble the complex due to the absence of any one of its components, results in the incorrect redistribution of the remaining components to diverse membrane compartments. KEYWORDS: Cell cycle;Cell division;Centromere;Chromosome;Cytoplasm;Cytoskeleton;Kinetochore;Mitosis;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Chromosome, centromere, kinetochore Cytoplasm, cytoskeleton, spindle Note=Dynamic pattern of localization during the cell cycle. In most cells at interphase, present diffusely in the cytoplasm. At metaphase, detected at the kinetochores and kinetochore microtubules, and in late anaphase and telophase accumulates at the spindle envelope midzone. " Q9VAJ3,"PROTEIN NAMES: Pickpocket protein 19 (PPK19) PROTEIN FAMILY: Amiloride-sensitive sodium channel family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family. FUNCTION: Part of a complex that plays a role in tracheal liquid clearance. In both larvae and adults, contributes to the behavioral response to salt. Probable role in sodium transport. KEYWORDS: Ion channel;Ion transport;Membrane;Reference proteome;Sodium;Sodium channel;Sodium transport;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Membrane ; Multi-pass membrane protein " Q9VB68,"PROTEIN NAMES: Serine protease grass (Gram-positive specific serine protease) PROTEIN FAMILY: Peptidase S1 family, CLIP subfamily ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the peptidase S1 family. CLIP subfamily. FUNCTION: Endopeptidase (By similarity). Plays a key role in innate immunity by activating the Toll pathway in response to fungal and Gram-positive bacterial infections, presumably downstream of pattern-recognition receptors (PRR), such as PGRP-SA, GNBP1 and GNBP3, and upstream of spz processing enzyme SPE. KEYWORDS: 3D-structure;Alternative splicing;Calcium;Direct protein sequencing;Disulfide bond;Glycoprotein;Hydrolase;Immunity;Innate immunity;Metal-binding;Protease;Reference proteome;Secreted;Serine protease;Signal;Zymogen SUBCELLULAR LOCATION: Secreted Note=Probably secreted in the hemolymph. " Q9VBP9,"PROTEIN NAMES: Nuclear protein localization protein 4 homolog PROTEIN FAMILY: NPL4 family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the NPL4 family. FUNCTION: May be part of a complex that binds ubiquitinated proteins and that is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. PATHWAY: Protein degradation; proteasomal ubiquitin-dependent pathway. KEYWORDS: 3D-structure;Alternative splicing;Metal-binding;Phosphoprotein;Reference proteome;Ubl conjugation pathway;Zinc;Zinc-finger " Q9VCC0,"PROTEIN NAMES: Cysteine and histidine-rich domain-containing protein morgana ORGANISM: Drosophila melanogaster (Fruit fly) FUNCTION: Regulates centrosome duplication and mitotic spindle dynamics. Also involved in controlling the size of dendritic arbors. May act as co-chaperone for Hsp83. During mitotic spindle assembly, regulates microtubule (MT) dynamics by binding to MTs and promoting MT polymerisation. Promotes the elongation and retraction of terminal branches in response to changes in body size, possibly acting downstream of the TORC2 pathway to enable proportional scaling of dendritic arbors. KEYWORDS: Cytoplasm;Cytoskeleton;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Repeat;Zinc SUBCELLULAR LOCATION: Cytoplasm Nucleus Cytoplasm, cytoskeleton, spindle Note=In syncytial embryos, mostly cytoplasmic during interphase, with enrichment in the perinuclear area and a weak nuclear localization. Upon nuclear envelope breakdown, localizes to the spindles and remains associated with the spindle microtubules throughout mitosis. " Q9VCP1,"PROTEIN NAMES: DNA repair protein Rad60 ORGANISM: Drosophila melanogaster (Fruit fly) FUNCTION: Required for repair of DNA double strand breaks which occur during replication or are induced by ionizing radiation (IR). Functions with dgrn and downstream of the SMC5-SMC6 complex to regulate strand break repair. Likely functions by stabilizing the association of heterochromatic double strand breaks (DSBs) with the nuclear periphery as part of the homologous recombination (HR) repair process. KEYWORDS: Alternative splicing;Nucleus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Nucleus Nucleus, nucleoplasm Note=Associates with the nuclear pores and localizes to the nuclear periphery. " Q9VCR6,"PROTEIN NAMES: Scaffold protein salvador (Shar-pei) ORGANISM: Drosophila melanogaster (Fruit fly) FUNCTION: Plays a key role in the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein Hippo (Hpo), in complex with its regulatory protein Salvador (Sav), phosphorylates and activates Warts (Wts) in complex with its regulatory protein Mats, which in turn phosphorylates and inactivates the Yorkie (Yki) oncoprotein. The Hippo/SWH signaling pathway inhibits the activity of the transcriptional complex formed by Scalloped (sd) and Yki and the target genes of this pathway include cyclin-E (cycE), diap1 and bantam. Required for cell cycle exit in eye imaginal disk and hid-induced apoptotic cell deaths that are part of normal retinal development. Activation of Drice in eye imaginal disk by either Hid or Rpr is almost completely blocked by Sav expression. KEYWORDS: 3D-structure;Apoptosis;Coiled coil;Phosphoprotein;Reference proteome;Repeat " Q9VCY8,"PROTEIN NAMES: Adiponectin receptor protein PROTEIN FAMILY: ADIPOR family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the ADIPOR family. FUNCTION: Adiponectin receptor. In insulin-producing cells, regulates insulin secretion and controls glucose and lipid metabolism. KEYWORDS: Alternative splicing;Cell membrane;Fatty acid metabolism;Lipid metabolism;Membrane;Metal-binding;Receptor;Reference proteome;Transmembrane;Transmembrane helix;Zinc SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein " Q9VDE6,"PROTEIN NAMES: Exocyst complex component 6 (Exocyst complex component Sec15) PROTEIN FAMILY: SEC15 family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the SEC15 family. FUNCTION: Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane. KEYWORDS: 3D-structure;Cell projection;Coiled coil;Exocytosis;Protein transport;Reference proteome;Transport SUBCELLULAR LOCATION: Cell projection, rhabdomere " Q9VDS5,PROTEIN NAMES: Rho GTPase-activating protein 92B ORGANISM: Drosophila melanogaster (Fruit fly) FUNCTION: GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state. KEYWORDS: Coiled coil;GTPase activation;Phosphoprotein;Reference proteome Q9VEI3,"PROTEIN NAMES: Adenosine 3'-phospho 5'-phosphosulfate transporter 1 (PAPS transporter 1) (Protein slalom) PROTEIN FAMILY: Nucleotide-sugar transporter family, SLC35B subfamily ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the nucleotide-sugar transporter family. SLC35B subfamily. FUNCTION: Mediates the transport of adenosine 3'-phospho 5'-phosphosulfate (PAPS), from cytosol into Golgi. PAPS is a universal sulfuryl donor for sulfation events that take place in the Golgi. Required for the dorsoventral patterning, suggesting that it mediates the transport of the sulfate donor required for the sulfotransferase activity of pip (pipe). KEYWORDS: Developmental protein;Golgi apparatus;Membrane;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Golgi apparatus membrane ; Multi-pass membrane protein " Q9VER6,"PROTEIN NAMES: Modular serine protease [Cleaved into: Modular serine protease non-catalytic chain; Modular serine protease catalytic chain] PROTEIN FAMILY: Peptidase S1 family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the peptidase S1 family. FUNCTION: Serine protease that plays a key role in innate immunity by activating the Toll pathway in response to infection with Gram-positive bacteria and fungi. During Gram-positive infection, acts downstream of PGRP-SA and upstream of Grass and Spz, and therefore appears to function in a pathway that links detection of Gram-positive lysine-type peptidoglycans to Toll activation. Functions in a separate pathway to the psh-mediated activation of the Toll pathway. KEYWORDS: Autocatalytic cleavage;Disulfide bond;Glycoprotein;Hydrolase;Immunity;Innate immunity;Protease;Reference proteome;Repeat;Secreted;Serine protease;Signal;Sushi;Zymogen SUBCELLULAR LOCATION: Secreted Note=Localizes at the membrane of lipid vesicles. Secreted from the fat body into the hemolymph at the surface of these lipid vesicles. " Q9VFC2,PROTEIN NAMES: Serine protease inhibitor 88Ea (Serpin 88Ea) PROTEIN FAMILY: Serpin family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the serpin family. FUNCTION: Serine protease inhibitor with activity toward trypsin. Negatively regulates the Toll signaling pathway and suppresses the expression of the antifungal peptide drosomycin. Its negative regulation of the Toll signaling pathway also results in the inhibition of the melanization immune response via the phenoloxidase (PPO1) cascade. Essential for unfolding and expansion of the wings after emergence from the pupal case. May regulate the Toll pathway by blocking the proteolysis of the Toll ligand spz. KEYWORDS: Glycoprotein;Immunity;Melanin biosynthesis;Protease inhibitor;Reference proteome;Secreted;Serine protease inhibitor;Signal SUBCELLULAR LOCATION: Secreted Q9VFP2,"PROTEIN NAMES: Protein roadkill (Hh-induced MATH and BTB domain-containing protein) PROTEIN FAMILY: Tdpoz family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the Tdpoz family. FUNCTION: Involved in segment polarity. In complex with gft/CUL3, promotes ubiquitination of ci and its subsequent degradation by the proteasome, which results in hh signaling attenuation. This regulation may be important during eye formation for proper packing of ommatidia into a hexagonal array. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Alternative splicing;Developmental protein;Nucleus;Reference proteome;Segmentation polarity protein;Ubl conjugation pathway SUBCELLULAR LOCATION: Nucleus " Q9VFS2,"PROTEIN NAMES: Carotenoid isomerooxygenase (Beta-carotene 15,15'-monooxygenase and retinoid isomerase) (Beta-carotene dioxygenase and retinoid isomerase) (Neither inactivation nor afterpotential mutant B) PROTEIN FAMILY: Carotenoid oxygenase family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the carotenoid oxygenase family. FUNCTION: Catalyzes the oxidative cleavage at the 15,15'-double bond of carotenoids and the simultaneous all-trans to 11-cis isomerization of one cleavage product. Carotenoids like 11-cis retinal can promote visual pigment biogenesis in the dark. Essential for the biosynthesis of the 3-hydroxyretinal chromophore of rhodopsin from zeaxanthin and for proper photoreceptor development. Also essential for larval light perception. PATHWAY: Cofactor metabolism; retinol metabolism. KEYWORDS: Iron;Metal-binding;Monooxygenase;Oxidoreductase;Reference proteome " Q9VGH1,"PROTEIN NAMES: Cytochrome P450 315a1, mitochondrial (CYPCCCXVA1) (Protein shadow) PROTEIN FAMILY: Cytochrome P450 family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the cytochrome P450 family. FUNCTION: Required for CNS development: midline glial cells. Involved in the metabolism of insect hormones: responsible for ecdysteroid C2-hydroxylase activity. May be involved in the breakdown of synthetic insecticides. PATHWAY: Steroid biosynthesis; ecdysteroid biosynthesis. KEYWORDS: Heme;Iron;Membrane;Metal-binding;Mitochondrion;Monooxygenase;Oxidoreductase;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Mitochondrion membrane MISCELLANEOUS: Member of the Halloween gene group." Q9VGL8,"PROTEIN NAMES: TBC1 domain family member whacked ORGANISM: Drosophila melanogaster (Fruit fly) FUNCTION: Essential for ensuring the polarized growth of tracheal seamless tubes. During seamless tube morphogenesis, likely to act as a GTPase-activating protein (GAP) for Rab35 to regulate vesicle trafficking from the recycling endosomes to the lumenal apical membrane to ensure the polarized dynein motor complex-dependent growth of seamless tubes along the proximodistal axis in tracheal terminal cells. When the terminal branch lumen is growing, Rab35-GTP is active and likely directs the transport of apical membrane vesicles from the soma to the distal tip of elongating terminal cell branches thus providing a continuous supply of apical membrane components as the lumen grows. Whereas when Rab35-GDP is inactivated, presumably by this GAP, apical membrane vesicles are transported to a central location adjacent to the terminal cell nucleus. KEYWORDS: Cell membrane;Cell projection;Cytoplasmic vesicle;GTPase activation;Membrane;Reference proteome SUBCELLULAR LOCATION: Apical cell membrane; Peripheral membrane protein Cytoplasmic vesicle Cell projection, filopodium Note=Restricted to the lumenal membrane of tracheal terminal cells with increased accumulation at the growing tips of seamless tubes. Colocalizes with Rab35 at the apical membrane, cytoplasmic puncta (likely to be vesicles) and filopodia. " Q9VGN7,PROTEIN NAMES: Exonuclease 3'-5' domain-containing protein 2 (dEXD2) PROTEIN FAMILY: EXD2 family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the EXD2 family. FUNCTION: 3'-5' exoribonuclease required for mitochondrial metabolism. KEYWORDS: Exonuclease;Hydrolase;Membrane;Metal-binding;Mitochondrion;Nuclease;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Mitochondrion membrane Q9VH14,"PROTEIN NAMES: Tissue inhibitor of metalloproteinase PROTEIN FAMILY: Protease inhibitor I35 (TIMP) family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the protease inhibitor I35 (TIMP) family. FUNCTION: Metalloproteinase inhibitor that acts on both matrix metalloproteinases Mmp1 and Mmp2 in vitro. Complexes with metalloproteinases and irreversibly inactivates them by binding to their catalytic zinc cofactor (By similarity). Required for wing maturation which is the final step in morphogenesis of the adult fly. Involved in the negative regulation of developmental tissue invasion for imaginal disk eversion during metamorphosis by inhibiting Mmp-mediated basement membrane (BM) degradation. Required for oogenesis and for the long-term maintainance of germarial structure and shape in the adult ovaries. Required for maintaining composition and biophysical properties of the extracellular matrix (ECM), and for the normal organization and cyst production of the germline stem cell (GSC) niche. KEYWORDS: Developmental protein;Differentiation;Direct protein sequencing;Disulfide bond;Metal-binding;Metalloenzyme inhibitor;Metalloprotease inhibitor;Oogenesis;Protease inhibitor;Reference proteome;Secreted;Signal;Zinc SUBCELLULAR LOCATION: Secreted " Q9VH90,"PROTEIN NAMES: Ubiquitin thioesterase trabid (dTrbd) PROTEIN FAMILY: Peptidase C64 family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the peptidase C64 family. FUNCTION: Positive regulator of the Wnt signaling pathway. Specifically cleaves 'Lys-63'-linked ubiquitin chains. May act by deubiquitinating APC protein, a negative regulator of Wnt-mediated transcription (By similarity). Required for an efficient wg response, but not for other signaling responses, in the eye. KEYWORDS: Hydrolase;Metal-binding;Phosphoprotein;Protease;Reference proteome;Repeat;Thiol protease;Ubl conjugation pathway;Wnt signaling pathway;Zinc;Zinc-finger " Q9VHR8,PROTEIN NAMES: Dipeptidyl peptidase 3 (Dipeptidyl aminopeptidase III) (Dipeptidyl arylamidase III) (Dipeptidyl peptidase III) (DPP III) PROTEIN FAMILY: Peptidase M49 family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the peptidase M49 family. FUNCTION: Degrades neuropeptide proctolin (RYLPT) by cleavage between Tyr and Leu residues. KEYWORDS: Alternative splicing;Aminopeptidase;Cell membrane;Cytoplasm;Hydrolase;Membrane;Metal-binding;Metalloprotease;Protease;Reference proteome;Transmembrane;Transmembrane helix;Zinc SUBCELLULAR LOCATION: [Isoform 1]: Membrane ; Multi-pass membrane protein.; SUBCELLULAR LOCATION: [Isoform 2]: Cell membrane ; Multi-pass membrane protein Cytoplasm Q9VI58,"PROTEIN NAMES: Terminal uridylyltransferase Tailor (TUTase Tailor) ORGANISM: Drosophila melanogaster (Fruit fly) FUNCTION: Uridylyltransferase which mediates terminal uridylation of miRNAs, leading to their degradation. Has high specificity for splicing-derived miRNAs (mirtrons) and other miRNA substrates containing a 3'-G terminal nucleotide. Appears to be a major suppressor of mirtron biogenesis. KEYWORDS: 3D-structure;ATP-binding;Cytoplasm;Magnesium;Metal-binding;Nucleotide-binding;Nucleotidyltransferase;Reference proteome;RNA-mediated gene silencing;Transferase SUBCELLULAR LOCATION: Cytoplasm " Q9VIF1,"PROTEIN NAMES: Exonuclease mut-7 homolog (Exonuclease 3'-5' domain-containing protein 3 homolog) (Protein nibbler) PROTEIN FAMILY: Mut-7 family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the mut-7 family. FUNCTION: Possesses 3'-5' exoribonuclease activity. Required for 3'-end trimming of AGO1-bound miRNAs, in particular multiple-isoform miRNAs, which represents a critical step in miRNA maturation. KEYWORDS: 3D-structure;Exonuclease;Hydrolase;Magnesium;Metal-binding;Nuclease;Phosphoprotein;Reference proteome " Q9VIF5,"PROTEIN NAMES: Protein deadlock ORGANISM: Drosophila melanogaster (Fruit fly) FUNCTION: Developmental protein involved in oogenesis. Required for germline maintenance, stability of mitotic spindles, localization of patterning determinants, oocyte growth and fusome biogenesis in males and females. Also required for dorso-ventral and antero-posterior patterning of oocyte and eggshell. May be involved in microtubule function during oogenesis. Part of a rhi-dependent transcription machinery that enables the generation of piRNA precursors from heterochromatin while maintaining the suppression of transposon-encoded promoters and enhancers. Component of the RDC complex (rhi, del and cuff) which binds to repressive H3K9me3 marks in the piRNA clusters. RDC promotes the bidirectional transcription of piRNA clusters at these sites by interacting with Moonshiner which forms a complex with the transcription initiation factors TfIIA-S and Trf2. This mechanism allows transcription to occur in piRNA clusters despite the lack of proper promoter elements and in the presence of the repressive H3K9me3 mark. As part of the RDC complex, involved in suppression of splicing. KEYWORDS: 3D-structure;Chromosome;Cytoplasm;Cytoskeleton;Developmental protein;Differentiation;Nucleus;Oogenesis;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Chromosome Note=Colocalizes with the oocyte nucleus at midstages of oogenesis and with the centrosomes of early embryos. Expressed in nuclear foci. All components of the Rhino-Deadlock-Cutoff (RDC) Complex associate with these nuclear foci and are required for their formation. " Q9VIH7,"PROTEIN NAMES: GTPase-activating protein skywalker ORGANISM: Drosophila melanogaster (Fruit fly) FUNCTION: GTPase-activating protein (GAP) for Rab35 which regulates synaptic vesicle (SV) protein recycling and turnover at the neuromuscular junction boutons and possibly ventral nerve cord via endosomal trafficking. Inhibits Rab35-mediated endosomal sorting which traffics old or dysfunctional SV proteins through a degradative endolysosomal route that involves the ESCRT pathway and the HOPS complex members dor, vps39 and rab7. This function is essential for preventing excessive degradation and turnover of vesicles from the readily releasable pool which leads to increased neurotransmission and eventually neurodegeneration. Preferentially binds phosphoinositides phosphorylated at the D5 position of the inositol ring, such as phosphatidylinositol 4,5-bisphosphate (PIP2) and phosphatidylinositol 3,4,5-trisphosphate (PIP3). Binding to phosphoinositides and thus membrane-association, is required for its function in regulating the turnover of synaptic-vesicle proteins. It is therefore likely that it is recruited to vesicle membranes with high phosphoinositide content and thereby selectively prevents endolysosomal degradation of these vesicles. KEYWORDS: 3D-structure;Alternative splicing;Cytoplasmic vesicle;Endosome;Lipid-binding;Membrane;Phosphoprotein;Reference proteome;Synapse SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane ; Peripheral membrane protein Endosome membrane ; Peripheral membrane protein Note=Detected at the synapses of neuromuscular junction boutons, where it displays co-localization with Rab35. Associates with certain types of membrane phosphoinositides such as phosphatidylinositol 4,5-bisphosphate (PIP2) and phosphatidylinositol 3,4,5-trisphosphate (PIP3). " Q9VIP8,PROTEIN NAMES: Protein valois (MEP50 homolog) ORGANISM: Drosophila melanogaster (Fruit fly) FUNCTION: Involved in specific localization of cytoplasmic proteins during the formation of pole plasm. Required for synthesis and/or stability of oskar protein (osk) and localization of tudor (tud) in both the nuage and posterior pole of the oocyte. Required for normal posterior localization of osk in later stages of oogenesis and for posterior localization of the vasa (vas) protein during the entire process of pole plasm assembly. May act by regulating the complex that contains the arginine N-methyltransferase csul. KEYWORDS: Cytoplasm;Developmental protein;Differentiation;Oogenesis;Reference proteome;Repeat;WD repeat SUBCELLULAR LOCATION: Cytoplasm Q9VIW3,"PROTEIN NAMES: Ran GTPase-activating protein (RanGAP) (Protein segregation distorter) PROTEIN FAMILY: RNA1 family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the RNA1 family. FUNCTION: GTPase activator for the nuclear Ras-related regulatory protein Ran, converting it to the putatively inactive GDP-bound state (By similarity). Trans-acting factor necessary for meiotic distortion. Distortion is only seen in individuals that carry the RanGAP tandem duplication and express a RanGAP truncated protein. Binding of truncated RanGAP product to the Responder(RSP) locus initiates events that lead to sperm dysfunction. During oogenesis, plays a role in the biogenesis of annulate lamellae containing nuclear pore complex components. KEYWORDS: Cytoplasm;GTPase activation;Leucine-rich repeat;Membrane;Nucleus;Phosphoprotein;Reference proteome;Repeat SUBCELLULAR LOCATION: Cytoplasm Nucleus membrane ; Peripheral membrane protein ; Cytoplasmic side Note=Association to cytoplasmic side of the nuclear pore complex is promoted by Nup214. Co-localizes with Nup358/RanBP2 to annulate lamellae. " Q9VJS8,PROTEIN NAMES: Zinc finger protein Elbow PROTEIN FAMILY: Elbow/Noc family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the Elbow/Noc family. FUNCTION: May negatively regulate Notch-induced cell proliferation in the eye-head primordium. May act in leg and wing primordia to negatively regulate body-wall specifying genes and thereby promote appendage formation. Required for tracheal development. KEYWORDS: Alternative splicing;Developmental protein;Metal-binding;Notch signaling pathway;Reference proteome;Zinc;Zinc-finger Q9VJV8,"PROTEIN NAMES: DNA polymerase subunit gamma-2, mitochondrial (DNA polymerase beta subunit) (DNA polymerase gamma 35kD subunit) (DNA polymerase gamma subunit 2) ORGANISM: Drosophila melanogaster (Fruit fly) FUNCTION: As accessory component of the DNA polymerase gamma complex is involved in the replication of mitochondrial DNA. Does not bind DNA. Essential for mitochondrial DNA maintenance and larval development. KEYWORDS: Direct protein sequencing;DNA replication;Mitochondrion;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Mitochondrion " Q9VK34,"PROTEIN NAMES: NAD-dependent histone deacetylase sirtuin-1 (Silent information regulator 2) PROTEIN FAMILY: Sirtuin family, Class I subfamily ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the sirtuin family. Class I subfamily. FUNCTION: NAD-dependent histone deacetylase involved in heterochromatic silencing. Mildly suppresses the heterochromatin-mediated silencing phenomenon known as position-effect variegation (PEV). Required for epigenetic silencing of the polycomb group proteins. Has histone H4 deacetylase activity in vitro. Required maternally for establishing proper segmentation of the embryo. Involved in sex determination. May be involved in the regulation of life span. KEYWORDS: Chromosome;Cytoplasm;Metal-binding;NAD;Nucleus;Phosphoprotein;Reference proteome;Repressor;Transcription;Transcription regulation;Transferase;Zinc SUBCELLULAR LOCATION: Cytoplasm Nucleus Chromosome " Q9VKD7,"PROTEIN NAMES: Mitochondrial cardiolipin hydrolase (Mitochondrial phospholipase) (MitoPLD) (Protein zucchini) PROTEIN FAMILY: Phospholipase D family, MitoPLD/Zucchini subfamily ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the phospholipase D family. MitoPLD/Zucchini subfamily. FUNCTION: Cardiolipin hydrolase present at the mitochondrial outer membrane required for piRNA metabolic process. Acts by catalyzing the hydrolysis of cardiolipin (diphosphatidylglycerol) to form phosphatidate (phosphatidic acid or PA) at the mitochondrial outer membrane surface, promoting the piRNA metabolic process. Plays a key role in primary biogenesis of piRNAs and is required during oogenesis to repress transposable elements and prevent their mobilization. piRNAs mediate the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Involved in trans-silencing effect (TSE), a homology-dependent repression mechanism by which a P-transgene inserted in subtelomeric heterochromatin via its role in piRNA biogenesis. KEYWORDS: 3D-structure;Differentiation;Hydrolase;Lipid degradation;Lipid metabolism;Meiosis;Membrane;Mitochondrion;Mitochondrion outer membrane;Oogenesis;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Mitochondrion outer membrane ; Single-pass membrane protein Note=Was initially reported to localize to the meiotic nuage, also named P granule. However, it was later shown that the EGFP tag at N-terminus used in initial experiments interfered with mitochondrial localization. " Q9VMG0,"PROTEIN NAMES: Arylalkylamine N-acetyltransferase-like 2 (AANAT2) (Arylalkylamine N-acyltransferase-like 2) PROTEIN FAMILY: Acetyltransferase family, AANAT subfamily ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the acetyltransferase family. AANAT subfamily. FUNCTION: Catalyzes the formation of long-chain N-acylserotonins and N-acyldopamines, which are important cellular signaling lipids. Catalyzes in vitro the formation of various N-acetyl-2-arylethylamines such as N-acetyltryptamine and melatonin. PATHWAY: Aromatic compound metabolism; melatonin biosynthesis; melatonin from serotonin: step 1/2.; PATHWAY: Lipid metabolism; fatty acid metabolism. KEYWORDS: Acyltransferase;Direct protein sequencing;Reference proteome;Transferase " Q9VML1,"PROTEIN NAMES: Protein dissatisfaction (Nuclear receptor subfamily 2 group E member 4) PROTEIN FAMILY: Nuclear hormone receptor family, NR2 subfamily ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the nuclear hormone receptor family. NR2 subfamily. FUNCTION: Orphan receptor that binds DNA as a monomer to hormone response elements (HRE) containing an extended core motif half-site sequence 5'-AAGTCA-3'. Acts as a transcriptional repressor. In both females and males, functions downstream of tra2 to regulate the development and function of certain sex-specific abdominal neurons, and likely regulates the development and/or function of the nervous system that controls courtship behaviors involved in the recognition and response of adults to appropriate sexual partners. In males, promotes abdominal motor neuronal innervation of the ventral muscles of abdominal segment 5 which is required for correct abdominal curling during copulation. In females, also promotes abdominal motor neuronal innervation but on the uterine muscles which is required for egg laying. KEYWORDS: DNA-binding;Metal-binding;Nucleus;Receptor;Reference proteome;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus " Q9VMU8,"PROTEIN NAMES: UDP-galactose transporter senju PROTEIN FAMILY: Nucleotide-sugar transporter family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the nucleotide-sugar transporter family. FUNCTION: UDP-galactose transporter involved in the synthesis of galactose-containing glycans. Plays a role in quiescence of the innate immune response, possibly by regulating glycosylation of the Toll pathway ligand spz. KEYWORDS: Golgi apparatus;Membrane;Reference proteome;Sugar transport;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Golgi apparatus membrane ; Multi-pass membrane protein MISCELLANEOUS: The gene name means 'Buddha with a thousand hands manipulating tools to protect us' in Japanese." Q9VMY9,"PROTEIN NAMES: Guanine deaminase (Dihydropterin deaminase) PROTEIN FAMILY: Metallo-dependent hydrolases superfamily, ATZ/TRZ family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the metallo-dependent hydrolases superfamily. ATZ/TRZ family. FUNCTION: Catalyzes the hydrolytic deamination of guanine, producing xanthine and ammonia. Also has 7,8-dihydropterin deaminase activity, which plays a role in synthesis of the red eye pigment aurodrosopterin. PATHWAY: Purine metabolism; guanine degradation; xanthine from guanine: step 1/1. KEYWORDS: Hydrolase;Metal-binding;Reference proteome;Zinc " Q9VN45,"PROTEIN NAMES: Protein spartin ORGANISM: Drosophila melanogaster (Fruit fly) FUNCTION: During postembryonic development, functions with endocytic adapter Eps-15 in neurons to restrain synaptic growth, by inhibiting BMP signaling, and to control synaptic endocytosis. Required presynaptically for neuromuscular junction (NMJ) neurotransmission. Inhibits neuronal BMP signaling by promoting endocytic internalization and subsequent endosomal trafficking of the BMP receptor wit. In this way, regulates the Fmr1 translational regulator controlling Futsch expression to modulate neuronal microtubule stability, which controls both synaptogenesis and neuronal survival. KEYWORDS: Cell membrane;Cell projection;Developmental protein;Endosome;Lipid droplet;Membrane;Neurodegeneration;Neurogenesis;Reference proteome;RNA editing;Synapse SUBCELLULAR LOCATION: Presynaptic cell membrane Early endosome Lipid droplet Note=Colocalizes with Eps-15 at presynaptic cell membrane. " Q9VNM1,"PROTEIN NAMES: Neuropeptide F receptor (DmNPFR1) PROTEIN FAMILY: G-protein coupled receptor 1 family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the G-protein coupled receptor 1 family. FUNCTION: Receptor for NPF. Integral part of the sensory system that mediates food signaling, providing the neural basis for the regulation of food response; coordinates larval foraging and social behavior changes during development. Required in dopaminergic (DA) neurons that innervate the mushroom body for satiety to suppress appetitive memory performance; a key factor in the internal state of hunger in the brain. NPF neurons coordinately modulate diverse sensory and motor neurons important for feeding, flight, and locomotion. NPF/NPFR pathway exerts its suppressive effect on larval aversion to diverse stressful stimuli (chemical stress and noxious heat) through attenuation of TRP channel-induced neuronal excitation. NPF neural signaling system plays a physiological role in acute modulation of alcohol sensitivity in adults, rather than a general response to intoxication by sedative agents. Activation and inhibition of the NPF system reduces and enhances ethanol preference, respectively. Sexual experience, the NPF system activity and ethanol consumption are all linked; sexual deprivation is a major contributor to enhanced ethanol preference. KEYWORDS: Alternative splicing;Behavior;Disulfide bond;G-protein coupled receptor;Membrane;Neuropeptide;Receptor;Reference proteome;Stress response;Transducer;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Multi-pass membrane protein " Q9VNX1,"PROTEIN NAMES: DNA polymerase eta PROTEIN FAMILY: DNA polymerase type-Y family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the DNA polymerase type-Y family. FUNCTION: DNA polymerase specifically involved in the DNA repair by translesion synthesis (TLS). Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Inserts one or 2 nucleotide(s) opposite the lesion. During homologous recombination (HR) repair, has a overlapping role with the error-prone translesion polymerase PolZ1/DNApol-zeta to initiate repair synthesis that is completed by end joining or another polymerase that can bind and reinitiate synthesis. Particularly important for the repair of UV-induced pyrimidine dimers and for hydroxyurea (HU)-induced DNA damage. Although inserts the correct base, may cause base transitions and transversions depending upon the context (By similarity). Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have any lyase activity, preventing the release of the 5'-deoxyribose phosphate (5'-dRP) residue (By similarity). This covalent trapping of the enzyme by the 5'-dRP residue inhibits its DNA synthetic activity during base excision repair, thereby avoiding high incidence of mutagenesis (By similarity). KEYWORDS: DNA damage;DNA repair;Magnesium;Manganese;Metal-binding;Nucleotidyltransferase;Nucleus;Reference proteome;Repeat;Transferase;Ubl conjugation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Note=Localizes to interphase nuclei but undetectable during mitosis. " Q9VPT5,"PROTEIN NAMES: Peroxisome assembly protein 12 (Peroxin-12) PROTEIN FAMILY: Pex2/pex10/pex12 family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the pex2/pex10/pex12 family. FUNCTION: Component of a retrotranslocation channel required for peroxisome organization by mediating export of the PEX5 receptor from peroxisomes to the cytosol, thereby promoting PEX5 recycling. The retrotranslocation channel is composed of PEX2, PEX10 and PEX12; each subunit contributing transmembrane segments that coassemble into an open channel that specifically allows the passage of PEX5 through the peroxisomal membrane (By similarity). PEX12 also regulates PEX5 recycling by activating the E3 ubiquitin-protein ligase activity of PEX10 (By similarity). When PEX5 recycling is compromised, PEX12 stimulates PEX10-mediated polyubiquitination of PEX5, leading to its subsequent degradation (By similarity). PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Membrane;Metal-binding;Peroxisome;Protein transport;Reference proteome;Transmembrane;Transmembrane helix;Transport;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Peroxisome membrane ; Multi-pass membrane protein " Q9VQ37,PROTEIN NAMES: Mitochondrial carrier protein Rim2 PROTEIN FAMILY: Mitochondrial carrier family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the mitochondrial carrier (TC 2.A.29) family. FUNCTION: Mitochondrial transporter that imports purine and pyrimidine nucleotides into the mitochondria. Essential for maintaining mitochondrial structure and function. Appears to be important for mitochondrial gene transcription and mitochondrial respiration. KEYWORDS: Membrane;Mitochondrion;Mitochondrion inner membrane;Reference proteome;Repeat;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Mitochondrion Mitochondrion inner membrane ; Multi-pass membrane protein Q9VQ56,"PROTEIN NAMES: Fez family zinc finger protein erm (Earmuff) PROTEIN FAMILY: Krueppel C2H2-type zinc-finger protein family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein family. FUNCTION: Zinc-finger transcriptional repressor. In larval brain, involved in the maintenance of cell fate of intermediate neural progenitors (INPs) that derive from type II neuroblasts. Restricts INP developmental potential and dedifferentiation by interacting with HDAC3 and the chromatin remodeling Brahma-associated protein (BAP) complex. Restricts INP proliferation by regulating neuroblast specific factors such as prospero, pnt and grh, and by antagonizing the function of self-renewal factors, such as klu, dpn and E(spl)mgamma-HLH. In the optic lobe, essential for coordinating the innervation/ targeting of the L3 and R8 axons to the M3 layer of the medulla. Early in medulla development, functions in parallel to CadN and Sema1a pathways to promote targeting of the L3 growth cones to the proximal domain of the outer medulla possibly by controlling the expression of various cell surface genes such as dpr1 and dpr17 which function in L3 growth cone targeting. Then once L3 growth cones segregate into the developing M3 layer, it activates the expression of netrins NetA and NetB which act locally to promote the attachment of R8 growth cones within the M3 layer. KEYWORDS: Developmental protein;Metal-binding;Neurogenesis;Nucleus;Reference proteome;Repeat;Repressor;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus " Q9VQ62,"PROTEIN NAMES: NPC intracellular cholesterol transporter 2 homolog a (Niemann Pick type C2 protein homolog) PROTEIN FAMILY: NPC2 family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the NPC2 family. FUNCTION: Functions redundantly with Npc2b in regulating sterol homeostasis and ecdysteroid biosynthesis, probably by controlling the availability of sterol substrate. KEYWORDS: Disulfide bond;Glycoprotein;Lipid biosynthesis;Lipid metabolism;Reference proteome;Secreted;Signal;Steroid biosynthesis SUBCELLULAR LOCATION: Secreted " Q9VQ91,"PROTEIN NAMES: Tudor and KH domain-containing protein homolog (Partner of PIWIs protein) PROTEIN FAMILY: Tdrkh family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the Tdrkh family. FUNCTION: Involved in the piwi-interacting RNA (piRNA) metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins, and governs the methylation and subsequent repression of transposons which is essential for germline integrity. Likely to act by recruiting Piwi proteins such as AGO3 and piwi to the piRNA biogenesis machinery in the nuage. Required for the final steps of primary piRNA biogenesis by participating in the 3' end-trimming of piwi-bound intermediates into mature piRNAs. KEYWORDS: 3D-structure;Cytoplasm;Nucleus;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Nucleus Cytoplasm, P-body Note=Component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon activity during meiosis. Expressed in the cytoplasm of germline stem cells, cyst cells, nurse cells and oocytes, with strong accumulation in region IIb germline cysts in the germarium. In post-germarium egg chambers accumulates in the perinuclear loci that appear to be the nuage. " Q9VQD6,PROTEIN NAMES: Early boundary activity protein 2 ORGANISM: Drosophila melanogaster (Fruit fly) FUNCTION: The heterotrimeric Elba complex is required for chromatin domain boundary function during early embryogenesis. It binds to a 8-bp sequence 5'-CCAATAAG-3' in the Fab-7 insulator or boundary element in the bithorax complex and contributes to its insulator or boundary activity. Elba2 can act as a transcriptional repressor and binds the palindromic sequence 5'-CCAATTGG-3' to mediate transcriptional repression. KEYWORDS: DNA-binding;Nucleus;Reference proteome;Repressor;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus Q9VQM4,"PROTEIN NAMES: Probable tyrosyl-DNA phosphodiesterase (Tyr-DNA phosphodiesterase) (Protein glaikit) PROTEIN FAMILY: Tyrosyl-DNA phosphodiesterase family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the tyrosyl-DNA phosphodiesterase family. FUNCTION: DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 3'-phosphodiester bond, giving rise to DNA with a free 3' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase I active site tyrosine residue. Hydrolyzes 3'-phosphoglycolates on protruding 3' ends on DNA double-strand breaks due to DNA damage by radiation and free radicals. Acts on blunt-ended double-strand DNA breaks and on single-stranded DNA. May have low 3'exonuclease activity and may be able to remove a single nucleoside from the 3'end of DNA and RNA molecules with 3'hydroxyl groups. Has no exonuclease activity towards DNA or RNA with a 3'phosphate (By similarity). Required for normal polarization of epidermal cells, correct subcellular location of the Crb complex to the apical lateral membrane, and for normal neuronal development during embryonic development. KEYWORDS: Cytoplasm;DNA damage;DNA repair;Exonuclease;Hydrolase;Nuclease;Nucleus;Reference proteome;Repeat SUBCELLULAR LOCATION: Nucleus Cytoplasm " Q9VRM7,PROTEIN NAMES: Cytochrome P450 307a1 (CYPCCCVIIA1) (Protein spook) PROTEIN FAMILY: Cytochrome P450 family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the cytochrome P450 family. FUNCTION: Required for correct development of the embryonic midline glial cells which are necessary for the formation of distinct segmental commissures. KEYWORDS: Developmental protein;Endoplasmic reticulum;Heme;Iron;Membrane;Metal-binding;Microsome;Monooxygenase;Oxidoreductase;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Peripheral membrane protein Microsome membrane ; Peripheral membrane protein MISCELLANEOUS: Member of the Halloween gene group. Q9VRQ7,PROTEIN NAMES: DNA polymerase epsilon subunit 2 (DNA polymerase II subunit 2) (DNA polymerase epsilon 58kD subunit) (DNA polymerase epsilon subunit) PROTEIN FAMILY: DNA polymerase epsilon subunit B family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the DNA polymerase epsilon subunit B family. FUNCTION: Accessory component of the DNA polymerase epsilon complex (By similarity). Participates in DNA repair and in chromosomal DNA replication (By similarity). Has a role in the entrance and progression through S phase. Has a role in endoreplication. Essential for viability and tissue development. KEYWORDS: DNA replication;DNA-binding;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus Q9VS05,"PROTEIN NAMES: Forkhead box protein biniou (Forkhead box protein FoxF) (DmFoxF) ORGANISM: Drosophila melanogaster (Fruit fly) FUNCTION: Component of a regulatory network controlling visceral mesoderm development and midgut morphogenesis. Transcriptional regulator involved in the activation of a large number of genes in the visceral mesoderm including betaTub60D, dpp and Hand. Binds to and regulates a number of enhancers driving expression in the visceral mesoderm in a temporally and spatially restricted manner. Also to binds to enhancers cooperatively with activators, such as bap or HLH54F, to coregulate expression of shared target genes in the visceral mesoderm. Binds to the Ndg enhancer and drives expression of Ndg in the late visceral musculature. May be involved in the transcriptional regulation of wupA in the visceral mesoderm. Plays an indirect role in the later stages of salivary gland positioning. KEYWORDS: Developmental protein;DNA-binding;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " Q9VSB9,PROTEIN NAMES: Transmembrane protein 43 homolog PROTEIN FAMILY: TMEM43 family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the TMEM43 family. FUNCTION: Involved in lipid metabolism and utilization. KEYWORDS: Endoplasmic reticulum;Membrane;Nucleus;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein Nucleus envelope Q9VSL3,"PROTEIN NAMES: Pyrimidodiazepine synthase (Protein sepia) PROTEIN FAMILY: GST superfamily, Omega family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the GST superfamily. Omega family. FUNCTION: Mediates the conversion of 2-amino-4-oxo-6-pyruvoyl-5,6,7,8-tetrahydropteridine (6-PTP; also named 6-pyruvoyltetrahydropterin) to 2-amino-6-acetyl-3,7,8,9-tetrahydro-3H-pyrimido(4,5-b)[1,4]diazepin-4-one (pyrimidodiazepine or PDA), a key intermediate in red eye pigment drosopterin biosynthesis. KEYWORDS: Oxidoreductase;Reference proteome " Q9VSR3,"PROTEIN NAMES: Translational regulator orb2 ORGANISM: Drosophila melanogaster (Fruit fly) FUNCTION: RNA-binding protein involved in translational regulation and required for long-term memory. Required in mushroom body gamma neurons for long-term memory in male courtship. Binds to mRNA 3'-UTRs. In its monomeric form, acts as a translational repressor of genes involved in neuronal growth, synapse formation and protein turnover. In its amyloid-like oligomeric form, acts as a translational activator. The monomeric form reduces poly(A) tail length and destabilizes mRNA while the oligomeric form protects and elongates the poly(A) tail and stabilizes mRNA. Involved in asymmetric cell division in the central nervous system. Plays a role in synapse formation and morphology at neuromuscular junctions by modulating the translation of the tumor suppressor brat. Required for the progression of spermatogenesis through meiosis and for sperm differentiation. During sperm differentiation, required to asymmetrically localize and activate the translation of protein kinase aPKC mRNAs which is necessary for spermatid cyst polarization. Also required during spermatid cyst polarization for localization and translation of its own mRNA.; FUNCTION: [Isoform A]: Required for initial memory acquisition. Following subsequent late dopaminergic pathway activation, recruits isoform B into a complex to activate translation of CaMKII which is required for long-term memory consolidation. KEYWORDS: 3D-structure;Activator;Alternative splicing;Amyloid;Cell cycle;Cell division;Cell projection;Cytoplasm;Differentiation;Phosphoprotein;Reference proteome;Repeat;Repressor;RNA-binding;Spermatogenesis;Synapse;Translation regulation SUBCELLULAR LOCATION: Perikaryon Cell projection, axon Cell projection, dendrite Synapse Cytoplasm Cytoplasm, perinuclear region Note=In embryonic and larval nervous system, concentrated in the perikaryon. In the adult central nervous system, localizes to synaptic terminals. In the ovary, localizes to the cytoplasm of nurse cells. Localizes to neuromuscular junctions. In spermatocytes, localizes throughout the cytoplasm with higher levels concentrated in a ring around the nucleus. The oligomeric form is enriched in the synaptic region.; SUBCELLULAR LOCATION: [Isoform A]: Synapse.; SUBCELLULAR LOCATION: [Isoform B]: Perikaryon Cell projection, axon Synapse MISCELLANEOUS: Lowering pH destabilizes the protein filaments." Q9VT65,PROTEIN NAMES: Calpain-B (Calcium-activated neutral proteinase B) (CANP B) [Cleaved into: Calpain-B catalytic subunit 1; Calpain-B catalytic subunit 2] PROTEIN FAMILY: Peptidase C2 family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the peptidase C2 family. FUNCTION: Calcium-regulated non-lysosomal thiol-protease. KEYWORDS: Autocatalytic cleavage;Calcium;Cytoplasm;Direct protein sequencing;Hydrolase;Membrane;Metal-binding;Protease;Reference proteome;Repeat;Thiol protease SUBCELLULAR LOCATION: Cytoplasm Membrane Note=Translocates to intracellular membranes when calcium levels are increased. Q9VT92,"PROTEIN NAMES: Odorant receptor 67d PROTEIN FAMILY: Insect chemoreceptor superfamily, Heteromeric odorant receptor channel family, Or67d subfamily ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the insect chemoreceptor superfamily. Heteromeric odorant receptor channel (TC 1.A.69) family. Or67d subfamily. FUNCTION: Plays a role in detection and sensitivity to pheromones and signal transduction of the fatty-acid-derived male pheromone 11-cis vaccenyl acetate (cVA). Acts in concert with Snmp and lush to capture cVA molecules on the surface of Or67d expressing olfactory dendrites and facilitate their transfer to the odorant-receptor Orco complex. Necessary to mediate behavioral responses to cVA by regulating both male and female mating behavior. Activation of Or67d neurons by cVA inhibits courtship of other males, whereas in females their activation promotes receptivity to other males. May form a complex with Orco to form odorant-sensing units, providing sensitive and prolonged odorant signaling and calcium permeability. KEYWORDS: Cell membrane;Membrane;Olfaction;Receptor;Reference proteome;Sensory transduction;Transducer;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein MISCELLANEOUS: The atypical heteromeric and topological design of the odorant receptors appears to be an insect-specific solution for odor recognition, making the OR/Orco complex an attractive target for the development of highly selective insect repellents to disrupt olfactory-mediated host-seeking behaviors of insect disease vectors. Odor-evoked OR currents are independent of known G-protein-coupled second messenger pathways." Q9VTH0,"PROTEIN NAMES: Procollagen-lysine,2-oxoglutarate 5-dioxygenase (Procollagen lysyl hydroxylase) ORGANISM: Drosophila melanogaster (Fruit fly) FUNCTION: Forms hydroxylysine residues in collagen type IV (By similarity). Required for the secretion of collagen type IV (vkg) from haemocytes, fat body and follicle cells. KEYWORDS: Dioxygenase;Endoplasmic reticulum;Glycoprotein;Iron;Metal-binding;Oxidoreductase;Reference proteome;Secreted;Signal;Vitamin C SUBCELLULAR LOCATION: Endoplasmic reticulum Secreted, extracellular space Note=Detected in the ER of fat body cells and haemocytes. Also detected in small puncta in the extracellular space, particularly in the region surrounding the haemocytes. " Q9VTL1,"PROTEIN NAMES: PCI domain-containing protein 2 homolog (CSN12-like protein) PROTEIN FAMILY: CSN12 family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the CSN12 family. FUNCTION: Required for the export of nuclear mRNAs and involved in mRNA trafficking in the cytoplasm. Component of the nuclear pore complex (NPC)-associated TREX-2/AMEX complex (anchoring and mRNA export complex) which functions in docking export-competent ribonucleoprotein particles (mRNPs) to the nuclear entrance of the nuclear pore complex (nuclear basket), thereby enabling the export of mRNAs to the cytoplasm through the nuclear pores. Within the complex, specifically promotes the association of factors involved in regulating nuclear mRNA export, such as Moe, sbr/NXF1 and the ORC complex, to the mRNPs particles. In the cytoplasm, functions independently of its role in the TREX-2/AMEX complex, to promote cytoplasmic mRNA trafficking together with nudC. Associates with translationally active polysomes. KEYWORDS: Cytoplasm;Cytoskeleton;Membrane;mRNA transport;Nucleus;Protein transport;Reference proteome;Translation regulation;Transport;Ubl conjugation SUBCELLULAR LOCATION: Nucleus Cytoplasm Nucleus membrane Cytoplasm, cytoskeleton Note=Shuttles in and out of the nucleus by a emb/Crm1-dependent mechanism. The ubiquitinated forms are localized to the cytoplasm, the nonubiquitinated forms are localized to the nucleus, and both forms are associated with the nuclear membrane. Associated with cytoplasmic microtubules. Associates with mRNA in the nucleus and cytoplasm. " Q9VTU3,PROTEIN NAMES: Rho GTPase-activating protein 68F ORGANISM: Drosophila melanogaster (Fruit fly) FUNCTION: Functions as a GTPase-activating protein (GAP) for RhoA/Rho1 during gastrulation by converting it to an inactive GDP-bound state. KEYWORDS: Developmental protein;Gastrulation;GTPase activation;Phosphoprotein;Reference proteome Q9VU68,"PROTEIN NAMES: Actin-interacting protein 1 (AIP1) (Protein flare) PROTEIN FAMILY: WD repeat AIP1 family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the WD repeat AIP1 family. FUNCTION: Induces disassembly of actin filaments in conjunction with ADF/cofilin family proteins. Together with GMF, promotes Arp2/3-nucleated actin filament array disassembly. Essential for organismal and cell viability. Required for the development of normal wing cell planar polarity. In egg chambers and together with GMF, plays an important role in directional migration of border cell clusters. KEYWORDS: Actin-binding;Alternative splicing;Cytoplasm;Cytoskeleton;Developmental protein;Reference proteome;Repeat;WD repeat SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton Note=At the cell periphery of wing cells. " Q9VUB4,"PROTEIN NAMES: GATOR complex protein NPRL3 (Nitrogen permease regulator 3-like protein) PROTEIN FAMILY: NPR3 family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the NPR3 family. FUNCTION: An essential component of the GATOR subcomplex GATOR1 which functions as an inhibitor of the amino acid-sensing branch of the TORC1 signaling pathway. The two GATOR subcomplexes, GATOR1 and GATOR2, regulate the TORC1 pathway in order to mediate metabolic homeostasis, female gametogenesis and the response to amino acid limitation and complete starvation. The function of GATOR1 in negatively regulating the TORC1 pathway is essential for maintaining baseline levels of TORC1 activity under nutrient rich conditions, and for promoting survival during amino acid or complete starvation by inhibiting TORC1-dependent cell growth and promoting catabolic metabolism and autophagy. In addition, this inhibition of TORC1 is necessary to maintain female fertility under normal conditions and during periods of nutrient stress. GATOR1 and GATOR2 act at different stages of oogenesis to regulate TORC1 in order to control meiotic entry and promote oocyte growth and development. After exactly four mitotic cyst divisions, the GATOR1 complex members (Iml1, Nprl2 and Nprl3) down-regulate TORC1 to slow cellular metabolism and promote the mitotic/meiotic transition. At later stages of oogenesis, the mio and Nup44A components of the GATOR2 complex inhibit GATOR1 and thus activate TORC1 to promote meiotic progression, and drive oocyte growth and development. KEYWORDS: Cell cycle;Cell division;Cytoplasm;Lysosome;Meiosis;Mitosis;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Lysosome Note=Localizes primarily to the autolysosomes during amino-acid starvation. " Q9VUF8,"PROTEIN NAMES: Ecdysone 20-monooxygenase (E20MO) (CYPCCCXIVA1) (Cytochrome P450 314a1, mitochondrial) (Protein shade) PROTEIN FAMILY: Cytochrome P450 family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the cytochrome P450 family. FUNCTION: Required for CNS development; midline glial cells. Involved in the metabolism of insect hormones; responsible for all ecdysone 20-monooxygenase activity during embryonic, larval and adult stages. May be involved in the breakdown of synthetic insecticides. PATHWAY: Steroid biosynthesis; ecdysteroid biosynthesis. KEYWORDS: Alternative splicing;Heme;Iron;Membrane;Metal-binding;Mitochondrion;Monooxygenase;Oxidoreductase;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Mitochondrion membrane MISCELLANEOUS: Member of the Halloween gene group." Q9VUK8,"PROTEIN NAMES: Glycine--tRNA ligase (Diadenosine tetraphosphate synthetase) (Glycyl-tRNA synthetase) PROTEIN FAMILY: Class-II aminoacyl-tRNA synthetase family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family. FUNCTION: Catalyzes the ATP-dependent ligation of glycine to the 3'-end of its cognate tRNA, via the formation of an aminoacyl-adenylate intermediate (Gly-AMP). Also produces diadenosine tetraphosphate (Ap4A), a universal pleiotropic signaling molecule needed for cell regulation pathways, by direct condensation of 2 ATPs. Thereby, may play a special role in Ap4A homeostasis (By similarity). Required for terminal arborization of both dendrites and axons during development. KEYWORDS: Alternative initiation;Aminoacyl-tRNA synthetase;ATP-binding;Cell projection;Cytoplasm;Ligase;Mitochondrion;Nucleotide-binding;Protein biosynthesis;Reference proteome;Transferase;Transit peptide SUBCELLULAR LOCATION: [Isoform B]: Mitochondrion.; SUBCELLULAR LOCATION: [Isoform A]: Cytoplasm Cell projection, axon " Q9VUT6,"PROTEIN NAMES: Polypeptide N-acetylgalactosaminyltransferase 8 (pp-GaNTase 8) (Protein-UDP acetylgalactosaminyltransferase 8) (UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 8) PROTEIN FAMILY: Glycosyltransferase 2 family, GalNAc-T subfamily ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily. FUNCTION: Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. It can both act as a peptide transferase that transfers GalNAc onto unmodified peptide substrates, and as a glycopeptide transferase that requires the prior addition of a GalNAc on a peptide before adding additional GalNAc moieties. Prefers both EA2 and the diglycosylated Muc5AC-3/13 as substrates, albeit at very low levels fro Muc5AC-3/13. PATHWAY: Protein modification; protein glycosylation. KEYWORDS: Disulfide bond;Glycoprotein;Glycosyltransferase;Golgi apparatus;Lectin;Manganese;Membrane;Metal-binding;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus membrane ; Single-pass type II membrane protein " Q9VV72,"PROTEIN NAMES: Multiple inositol polyphosphate phosphatase 1 (2,3-bisphosphoglycerate 3-phosphatase) (2,3-BPG phosphatase) PROTEIN FAMILY: Histidine acid phosphatase family, MINPP1 subfamily ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the histidine acid phosphatase family. MINPP1 subfamily. FUNCTION: Probable multiple inositol polyphosphate phosphatase that hydrolyzes 1D-myo-inositol 1,3,4,5,6-pentakisphosphate (InsP5[2OH]) and 1D-myo-inositol hexakisphosphate (InsP6) to a range of less phosphorylated inositol phosphates. This regulates the availability of these various small molecule second messengers and metal chelators which control many aspects of cell physiology. May have a dual substrate specificity, and function as a 2,3-bisphosphoglycerate 3-phosphatase hydrolyzing 2,3-bisphosphoglycerate to 2-phosphoglycerate. 2,3-bisphosphoglycerate (BPG) is formed as part of the Rapoport-Luebering glycolytic bypass (By similarity). Has a role in embryonic tracheal development where it localizes to the leading edge of actively migrating branches. In these leading cells, enhances formation and/or maintenance of filopodia which may drive branch migration and elongation by cell-cell intercalation. The function in tracheal morphogenesis is dependent on its inositol polyphosphate phosphatase activity. KEYWORDS: Cell junction;Cell membrane;Cell projection;Developmental protein;Glycoprotein;GPI-anchor;Hydrolase;Lipoprotein;Membrane;Reference proteome;Signal SUBCELLULAR LOCATION: Cell membrane ; Lipid-anchor, GPI-anchor Apical cell membrane Basolateral cell membrane Cell projection, filopodium Cell junction Note=Has a dynamic localization pattern during tracheal development. During early tracheal invagination (embryonic stage 10), localizes mainly to the apical cell membrane. During the primary tracheal branching stage (embryonic stage 11), found mainly at the basolateral cell membrane. At later stages (embryonic stage 13-15), also localizes to cell-cell junctions and filopodia. " Q9VVH9,"PROTEIN NAMES: Solute carrier organic anion transporter family member 74D (Ecdysone Importer) (EcI) (Organic anion transporting polypeptide 74D) PROTEIN FAMILY: Organo anion transporter family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the organo anion transporter (TC 2.A.60) family. FUNCTION: Transporter that mediates the cellular uptake of ecdysteroids, including ecdysone, from the hemolymph. KEYWORDS: Cell membrane;Disulfide bond;Glycoprotein;Ion transport;Membrane;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein " Q9VVW5,"PROTEIN NAMES: Dual specificity protein phosphatase Mpk3 (Drosophila MKP3) (DMKP3) (Mitogen-activated protein kinase phosphatase 3) (MAP kinase phosphatase 3) (MKP-3) PROTEIN FAMILY: Protein-tyrosine phosphatase family, Non-receptor class dual specificity subfamily ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily. FUNCTION: Negatively regulates the activity of members of the MAP kinase family in response to changes in the cellular environment. Has a specificity for the ERK family. Acts as a negative regulator in a variety of developmental processes including cell differentiation and proliferation controlled by the Ras/ERK pathway. Suppresses the photoreceptor cell differentiation and wing vein formation. Required for proper oogenesis and early embryogenesis. Functions autonomously in a subset of photoreceptor progenitor cells in eye imaginal disks. Appears also to be required in surrounding non-neuronal cells for ommatidial patterning and photoreceptor differentiation. Plays a role in the maintenance of epithelial integrity during tracheal development. KEYWORDS: Alternative splicing;Cytoplasm;Hydrolase;Protein phosphatase;Reference proteome SUBCELLULAR LOCATION: Cytoplasm " Q9VVX0,"PROTEIN NAMES: Protein Gemin2 PROTEIN FAMILY: Gemin-2 family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the gemin-2 family. FUNCTION: The SMN complex catalyzes the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome, and thereby plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP (Sm core) (By similarity). In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG (5Sm) are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP (By similarity). To assemble core snRNPs, the SMN complex accepts the trapped 5Sm proteins from CLNS1A (By similarity). Binding of snRNA inside 5Sm ultimately triggers eviction of the SMN complex, thereby allowing binding of SNRPD3 and SNRPB to complete assembly of the core snRNP (By similarity). Within the SMN complex, GEMIN2 constrains the conformation of 5Sm, thereby promoting 5Sm binding to snRNA containing the snRNP code (a nonameric Sm site and a 3'-adjacent stem-loop), thus preventing progression of assembly until a cognate substrate is bound (By similarity). KEYWORDS: 3D-structure;Cytoplasm;mRNA processing;mRNA splicing;Reference proteome;Spliceosome SUBCELLULAR LOCATION: Cytoplasm Note=Component of U bodies. " Q9VWF4,"PROTEIN NAMES: Dual specificity protein phosphatase MPK-4 PROTEIN FAMILY: Protein-tyrosine phosphatase family, Non-receptor class dual specificity subfamily ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily. FUNCTION: Dual specificity phosphatase; can dephosphorylate both phosphotyrosine and phosphoserine or phosphothreonine residues. May suppress bsk/JNK activation during the immune response. KEYWORDS: Cytoplasm;Hydrolase;Nucleus;Protein phosphatase;Reference proteome SUBCELLULAR LOCATION: Nucleus Cytoplasm Note=Mainly found in the nucleus. " Q9VWR5,PROTEIN NAMES: Cytochrome P450 306a1 (CYPCCCVIA1) (Protein phantom) (Dmphm) PROTEIN FAMILY: Cytochrome P450 family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the cytochrome P450 family. FUNCTION: Involved in the metabolism of insect hormones; responsible for ecdysteroid C25-hydroxylase activity. May be involved in the breakdown of synthetic insecticides. PATHWAY: Steroid biosynthesis; ecdysteroid biosynthesis. KEYWORDS: Endoplasmic reticulum;Heme;Iron;Membrane;Metal-binding;Microsome;Monooxygenase;Oxidoreductase;Reference proteome SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Peripheral membrane protein Microsome membrane ; Peripheral membrane protein MISCELLANEOUS: Member of the Halloween gene group. Q9VWU1,"PROTEIN NAMES: Serine protease persephone PROTEIN FAMILY: Peptidase S1 family, CLIP subfamily ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the peptidase S1 family. CLIP subfamily. FUNCTION: Serine protease that plays a key role in innate immunity in response to Gram-positive bacterial and fungal proteases. Acts as a component of the Toll pathway upstream of protease spz processing enzyme SPE and Tl ligand spz. Nec regulates the cascade by inhibiting psh. KEYWORDS: Disulfide bond;Glycoprotein;Hydrolase;Immunity;Innate immunity;Protease;Reference proteome;Secreted;Serine protease;Signal SUBCELLULAR LOCATION: Secreted " Q9VWV9,"PROTEIN NAMES: Protein-serine O-palmitoleoyltransferase porcupine PROTEIN FAMILY: Membrane-bound acyltransferase family, Porcupine subfamily ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the membrane-bound acyltransferase family. Porcupine subfamily. FUNCTION: Protein-serine O-palmitoleoyltransferase that acts as a key regulator of the Wnt signaling pathway by mediating the attachment of palmitoleate, a 16-carbon monounsaturated fatty acid (C16:1(9Z)), to Wnt proteins. Serine palmitoleoylation of Wnt proteins is required for efficient binding to frizzled receptors (By similarity). Also facilitates the glycosylation of Wnt family members, including wg and Wnt5. The cotranslational disulfide bond formation of wg competes with the N-glycosylation. Porc stimulates the post-translational N-glycosylation by anchoring wg at the ER membrane, probably through acylation. KEYWORDS: Acyltransferase;Endoplasmic reticulum;Membrane;Reference proteome;Transferase;Transmembrane;Transmembrane helix;Wnt signaling pathway SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein " Q9VXA0,"PROTEIN NAMES: GATOR complex protein NPRL2 (Nitrogen permease regulator 2-like protein) PROTEIN FAMILY: NPR2 family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the NPR2 family. FUNCTION: An essential component of the GATOR subcomplex GATOR1 which functions as an inhibitor of the amino acid-sensing branch of the TORC1 signaling pathway. The two GATOR subcomplexes, GATOR1 and GATOR2, regulate the TORC1 pathway in order to mediate metabolic homeostasis, female gametogenesis and the response to amino acid limitation and complete starvation. The function of GATOR1 in negatively regulating the TORC1 pathway is essential for maintaining baseline levels of TORC1 activity under nutrient rich conditions, and for promoting survival during amino acid or complete starvation by inhibiting TORC1-dependent cell growth and promoting catabolic metabolism and autophagy. In addition, this inhibition of TORC1 is necessary to maintain female fertility under normal conditions and during periods of nutrient stress. GATOR1 and GATOR2 act at different stages of oogenesis to regulate TORC1 in order to control meiotic entry and promote oocyte growth and development. After exactly four mitotic cyst divisions, the GATOR1 complex members (Iml1, Nprl2 and Nprl3) down-regulate TORC1 to slow cellular metabolism and promote the mitotic/meiotic transition. At later stages of oogenesis, the mio and Nup44A components of the GATOR2 complex inhibit GATOR1 and thus activate TORC1 to promote meiotic progression, and drive oocyte growth and development. KEYWORDS: Cell cycle;Cell division;Cytoplasm;Lysosome;Meiosis;Mitosis;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Lysosome Note=Localizes primarily to the autolysosomes during amino-acid starvation. " Q9VXD9,"PROTEIN NAMES: Probable G-protein coupled receptor Mth-like 1 (Protein methuselah-like 1) PROTEIN FAMILY: G-protein coupled receptor 2 family, Mth subfamily ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the G-protein coupled receptor 2 family. Mth subfamily. KEYWORDS: Cell membrane;Disulfide bond;G-protein coupled receptor;Glycoprotein;Membrane;Receptor;Reference proteome;Signal;Transducer;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein " Q9VXE5,"PROTEIN NAMES: Serine/threonine-protein kinase PAK mbt (Protein mushroom bodies tiny) (p21-activated kinase-related protein) PROTEIN FAMILY: Protein kinase superfamily, STE Ser/Thr protein kinase family, STE20 subfamily ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. FUNCTION: Involved in neurogenesis of the adult central nervous system, and together with Cdc42, regulates photoreceptor cell morphogenesis. Phosphorylates exogenous substrates when activated by Cdc42. KEYWORDS: ATP-binding;Cell junction;Cell membrane;Developmental protein;Differentiation;Kinase;Magnesium;Membrane;Neurogenesis;Nucleotide-binding;Phosphoprotein;Reference proteome;Serine/threonine-protein kinase;Transferase SUBCELLULAR LOCATION: Cell junction, adherens junction Cell membrane ; Peripheral membrane protein Note=Apical membrane sites of adherens junctions of developing photoreceptor cells. " Q9VYB7,PROTEIN NAMES: Protein-tyrosine sulfotransferase (Transport and Golgi organization protein 13) (Tango-13) (Tyrosylprotein sulfotransferase) PROTEIN FAMILY: Protein sulfotransferase family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the protein sulfotransferase family. FUNCTION: Catalyzes the O-sulfation of tyrosine residues within acidic motifs of polypeptides (By similarity). Has a role in protein secretion. KEYWORDS: Alternative splicing;Disulfide bond;Glycoprotein;Golgi apparatus;Membrane;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus membrane ; Single-pass type II membrane protein Q9VYM3,"PROTEIN NAMES: Tectonic-like complex member Mks1 (Meckel syndrome type 1 protein homolog) ORGANISM: Drosophila melanogaster (Fruit fly) FUNCTION: Probable component of the tectonic-like complex (also named MKS complex), a complex localized at the transition zone of primary cilia. Required for ciliary structure and function. KEYWORDS: Cell projection;Cilium biogenesis/degradation;Cytoplasm;Cytoskeleton;Reference proteome SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, cilium basal body Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole Note=Localizes at the transition zone (TZ), a region between the basal body and the ciliary axoneme in the olfactory, auditory and speromatocyte system. In spermatocytes, localizes in the transition zone and the migrating base of the spermatid ciliary cap. Co-localizes with the tectonic-like complex. " Q9VYV3,PROTEIN NAMES: Thioredoxin domain-containing protein 5 homolog (Protein pretaporter) PROTEIN FAMILY: Protein disulfide isomerase family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the protein disulfide isomerase family. FUNCTION: Possesses thioredoxin activity (By similarity). Acts as a ligand for Drpr and is required for the phagocytosis of apoptotic cells. Binds to the extracellular region of Drpr and augments Drpr tyrosine phosphorylation. KEYWORDS: Alternative splicing;Disulfide bond;Endoplasmic reticulum;Phagocytosis;Redox-active center;Reference proteome;Repeat;Signal SUBCELLULAR LOCATION: Endoplasmic reticulum Cell surface Note=Relocates from the endoplasmic reticulum to the cell surface during apoptosis. Q9VZ49,"PROTEIN NAMES: Endoribonuclease CG2145 (Poly(U)-specific endoribonuclease homolog) (Uridylate-specific endoribonuclease homolog) PROTEIN FAMILY: ENDOU family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the ENDOU family. FUNCTION: Catalyzes RNA cleavage releasing a product with a 2',3'-cyclic phosphate at the 3'-end. KEYWORDS: Endonuclease;Hydrolase;Lyase;Manganese;Metal-binding;Nuclease;Reference proteome;RNA-binding;Secreted;Signal SUBCELLULAR LOCATION: Secreted " Q9VZL7,"PROTEIN NAMES: Gustatory and odorant receptor 63a PROTEIN FAMILY: Insect chemoreceptor superfamily, Gustatory receptor (GR) family, Gr21a subfamily ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the insect chemoreceptor superfamily. Gustatory receptor (GR) family. Gr21a subfamily. FUNCTION: Gustatory and odorant receptor which mediates acceptance or avoidance behavior, depending on its substrates. Gr21a and Gr63a together are sufficient for carbon dioxide detection and avoidance behavior. It is possible that the CO(2) receptors Gr63a and Gr21a activate the TRPC channels through Galpha49B and Plc21C. This innate olfactory avoidance behavior can be inhibited by inhibitory interactions of the odors such as 1-hexanol and 2,3-butanedione with Gr21a and Gr63a. KEYWORDS: Behavior;Cell membrane;Glycoprotein;Membrane;Olfaction;Receptor;Reference proteome;Sensory transduction;Transducer;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein " Q9VZW5,"PROTEIN NAMES: FMRFamide receptor (DFR) (DrmFMRFa-R) PROTEIN FAMILY: G-protein coupled receptor 1 family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the G-protein coupled receptor 1 family. FUNCTION: A receptor for the FMRFamide peptides. Reacts with high affinity to FMRFamide and intrinsic FMRFamide-related peptides. By stimulating intracellular calcium signaling through the inositol 1,4,5-trisphosphate receptor, Itpr, in dopaminergic neurons, may be involved in the maintenance of neuronal excitability and in the regulation of flight bout duration. KEYWORDS: Cell membrane;G-protein coupled receptor;Glycoprotein;Membrane;Receptor;Reference proteome;Transducer;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. " Q9W032,PROTEIN NAMES: Protein ecdysoneless PROTEIN FAMILY: ECD family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the ECD family. FUNCTION: Required in both the follicle cells and the germline for oocyte development. KEYWORDS: Cytoplasm;Developmental protein;Differentiation;Oogenesis;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Q9W0G1,"PROTEIN NAMES: Tyrosine-protein phosphatase non-receptor type 61F (dPTP61F) PROTEIN FAMILY: Protein-tyrosine phosphatase family, Non-receptor class 1 subfamily ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily. FUNCTION: Non-receptor protein tyrosine phosphatase. Required for maintaining dock/dreadlocks in its non-phosphorylated state. Negative regulator of InR/insulin-like receptor signaling through dephosphorylation of tyrosines when recruited by dock/dreadlocks. KEYWORDS: Alternative splicing;Cytoplasm;Hydrolase;Membrane;Nucleus;Phosphoprotein;Protein phosphatase;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: [Isoform A]: Cytoplasm. Membrane ; Single-pass membrane protein Endomembrane system. Note=Associates with the membranes of the reticular network and the mitochondria.; SUBCELLULAR LOCATION: [Isoform B]: Nucleus. " Q9W0I6,"PROTEIN NAMES: Phosphatidylinositol-3-phosphatase SAC1 (Phosphatidylinositol-4-phosphate phosphatase) (Suppressor of actin mutations 1-like protein) ORGANISM: Drosophila melanogaster (Fruit fly) FUNCTION: Phosphoinositide phosphatase which catalyzes the hydrolysis of phosphatidylinositol 3-phosphate (PtdIns(3)P) and phosphatidylinositol 4-phosphate (PtdIns(4)P) (By similarity). Has low activity towards phosphatidylinositol-3,5-bisphosphate (PtdIns(3,5)P2) (By similarity). KEYWORDS: Endoplasmic reticulum;Golgi apparatus;Hydrolase;Membrane;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein Golgi apparatus membrane ; Multi-pass membrane protein " Q9W0K7,PROTEIN NAMES: Protein bric-a-brac 1 ORGANISM: Drosophila melanogaster (Fruit fly) FUNCTION: Probably acts as a transcriptional regulator. Required for the specification of the tarsal segment. Also involved in antenna development. KEYWORDS: DNA-binding;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: 'Bric-a-brac' means 'jumble' in French (referring to the mutant ovary phenotype). Q9W0Y6,PROTEIN NAMES: Transient receptor potential cation channel protein painless PROTEIN FAMILY: Transient receptor family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the transient receptor (TC 1.A.4) family. FUNCTION: Receptor-activated non-selective cation channel involved in detection of pain sensation due to high temperature. Involved in heat nociception by being activated by noxious temperature of 38 degrees Celsius. KEYWORDS: ANK repeat;Ion channel;Ion transport;Membrane;Reference proteome;Repeat;Sensory transduction;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Membrane ; Multi-pass membrane protein Q9W197,PROTEIN NAMES: 7-methylguanosine phosphate-specific 5'-nucleotidase (7-methylguanosine nucleotidase) (Cytosolic 5'-nucleotidase IIIB) (N(7)-methylguanylate 5'-phosphatase) PROTEIN FAMILY: Pyrimidine 5'-nucleotidase family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the pyrimidine 5'-nucleotidase family. FUNCTION: Specifically hydrolyzes 7-methylguanosine monophosphate (m(7)GMP) to 7-methylguanosine and inorganic phosphate. Also able to mediate hydrolysis of diphosphate (m(7)GDP) to 7-methylguanosine and 2 inorganic phosphate with lower activity. The specific activity for m(7)GMP may protect cells against undesired salvage of m(7)GMP and its incorporation into nucleic acids. Also has weak activity for CMP. UMP and purine nucleotides are poor substrates. KEYWORDS: 3D-structure;Hydrolase;Magnesium;Metal-binding;Nucleotide metabolism;Nucleotide-binding;Reference proteome Q9W1I9,"PROTEIN NAMES: Inactive peptidyl-prolyl cis-trans isomerase shutdown PROTEIN FAMILY: FKBP6 family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the FKBP6 family. FUNCTION: Co-chaperone required during oogenesis to repress transposable elements and prevent their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Acts as a co-chaperone via its interaction with Hsp83/HSP90 and is required for the biogenesis of all three piRNA major populations. KEYWORDS: Cytoplasm;Differentiation;Meiosis;Oogenesis;Reference proteome;Repeat;RNA-mediated gene silencing;TPR repeat SUBCELLULAR LOCATION: Cytoplasm Note=Present in the cytoplasm of germ cells. Component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon activity during meiosis. Also present in Yb bodies. " Q9W354,"PROTEIN NAMES: Extracellular signal-regulated kinase 7 PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. FUNCTION: Atypical MAPK protein that regulates protein secretion in a kinase activity-dependent manner. In response to starvation regulates protein secretion by mediating transitional endoplasmic reticulum site disassembly. Mediates inhibition of insulin-like peptide secretion upon disturbed ribosome biogenesis and acts as a downstream effector of TP53. KEYWORDS: Alternative splicing;ATP-binding;Kinase;Nucleotide-binding;Reference proteome;Serine/threonine-protein kinase;Transferase " Q9W3C7,"PROTEIN NAMES: Palmitoyl-protein thioesterase 1 (PPT-1) (Palmitoyl-protein hydrolase 1) PROTEIN FAMILY: Palmitoyl-protein thioesterase family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the palmitoyl-protein thioesterase family. FUNCTION: Cleaves thioester-linked long fatty acyl groups such as palmitate from modified cysteine residues in proteins or peptides. KEYWORDS: Disulfide bond;Glycoprotein;Hydrolase;Lysosome;Reference proteome;Signal SUBCELLULAR LOCATION: Lysosome MISCELLANEOUS: Flies deficient in Ppt1 are viable and fertile, but accumulate abnormal autofluorescent storage material in the adult central nervous system and have a shorter life span." Q9W3Y4,"PROTEIN NAMES: GAS2-like protein pickled eggs PROTEIN FAMILY: GAS2 family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the GAS2 family. FUNCTION: Essential for development and viability. Required for ovary development and oogenesis, and is essential for the development of the indirect flight muscles. May act as a negative regulator of the Notch signaling pathway in certain tissues, such as the muscle precursors and ovaries. May function as a linker protein between the actin and microtubule cytoskeletons. KEYWORDS: Cytoplasm;Cytoskeleton;Reference proteome SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton Cytoplasm, cell cortex Note=In the cytoskeleton associates with both actin and the microtubules. MISCELLANEOUS: Named 'Picked eggs' based upon the mutant ovary phenotype." Q9W429,"PROTEIN NAMES: UDP-xylose and UDP-N-acetylglucosamine transporter (ER GDP-fucose transporter) (Solute carrier family 35 member B4 homolog) PROTEIN FAMILY: Nucleotide-sugar transporter family, SLC35B subfamily ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the nucleotide-sugar transporter family. SLC35B subfamily. FUNCTION: Sugar transporter that specifically mediates the transport of UDP-N-acetylglucosamine (UDP-GlcNAc), GDP-fucose and UDP-xylose. Functions redundantly with nac in the O-fucosylation of Notch, positively regulating Notch signaling. Involved in the biosynthesis of heparan sulfate-glycosaminoglycan (HS-GAG) and in Dpp signaling in the wing imaginal disk. KEYWORDS: Endoplasmic reticulum;Membrane;Reference proteome;Sugar transport;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein " Q9W462,PROTEIN NAMES: Ubiquitin carboxyl-terminal hydrolase 30 homolog (dUSP30) (Deubiquitinating enzyme 30 homolog) (Ubiquitin thioesterase 30 homolog) (Ubiquitin-specific-processing protease 30 homolog) (Ub-specific protease 30 homolog) PROTEIN FAMILY: Peptidase C19 family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the peptidase C19 family. FUNCTION: Deubiquitinating enzyme that acts as a key inhibitor of mitophagy by counteracting the action of parkin (park). KEYWORDS: Hydrolase;Membrane;Mitochondrion;Mitochondrion outer membrane;Protease;Reference proteome;Thiol protease;Transmembrane;Transmembrane helix;Ubl conjugation pathway SUBCELLULAR LOCATION: Mitochondrion outer membrane Q9W497,"PROTEIN NAMES: Gustatory receptor 5a for trehalose (Trehalose receptor) PROTEIN FAMILY: Insect chemoreceptor superfamily, Gustatory receptor (GR) family, Gr5a subfamily ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the insect chemoreceptor superfamily. Gustatory receptor (GR) family. Gr5a subfamily. FUNCTION: Gustatory receptor required for response to the sugar trehalose in taste neurons. Gr5a neurons selectively respond to sugars, in contrast to Gr66a cells which respond to bitter compounds. Flies are attracted to sugars and avoid bitter substances, suggesting that Gr5a neuron activity is sufficient to mediate acceptance behavior. Sugar signal transduction occurs through coupling with G-proteins such as Galpha49B and G-salpha60A. KEYWORDS: Cell membrane;Membrane;Receptor;Reference proteome;Transducer;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein MISCELLANEOUS: Variant Thr-218 was found to be the ancestral form in D.melanogaster, suggesting that low trehalose sensitivity was an ancestral form with respect to the receptor function." Q9W4D2,PROTEIN NAMES: RNA-binding protein 4F ORGANISM: Drosophila melanogaster (Fruit fly) FUNCTION: May be involved in gene regulation during development. Binds RNA. KEYWORDS: Alternative splicing;Cytoplasm;Developmental protein;Phosphoprotein;Reference proteome;RNA editing;RNA-binding SUBCELLULAR LOCATION: Cytoplasm MISCELLANEOUS: [Isoform A]: Intron retention event in non-edited RNA.; MISCELLANEOUS: [Isoform B]: Intron excised in non-edited RNA. Q9W4M9,"PROTEIN NAMES: tRNA (cytosine(34)-C(5))-methyltransferase (NOP2/Sun domain family member 2 ortholog) PROTEIN FAMILY: Class I-like SAM-binding methyltransferase superfamily, RsmB/NOP family, TRM4 subfamily ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family. TRM4 subfamily. FUNCTION: RNA methyltransferase that methylates tRNAs. Methylates cytosine to 5-methylcytosine (m5C) at position 34 of intron-containing tRNA(Leu)(CAA) precursors. Required for short-term memory. KEYWORDS: Methyltransferase;Nucleus;Phosphoprotein;Reference proteome;RNA-binding;S-adenosyl-L-methionine;Transferase;tRNA processing;tRNA-binding SUBCELLULAR LOCATION: Nucleus, nucleolus " Q9W4N2,"PROTEIN NAMES: Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 1 (Cap1 2'O-ribose methyltransferase 1) (MTr1) ORGANISM: Drosophila melanogaster (Fruit fly) FUNCTION: S-adenosyl-L-methionine-dependent methyltransferase that mediates mRNA cap1 2'-O-ribose methylation to the 5'-cap structure of mRNAs. Methylates the ribose of the first nucleotide of a m(7)GpppG-capped mRNA to produce m(7)GpppNmp (cap1). Positively regulates the Ago2-dependent small RNA pathway, with roles in both siRNA biogenesis and RISC assembly. Involved in facilitating conversion of pre-RISC into holo-RISC, possibly by promoting the unwinding of Ago2-bound siRNA duplexes and thus the retention of the guide strand in holo-RISC. KEYWORDS: Cytoplasm;Methyltransferase;mRNA capping;mRNA processing;Nucleus;Reference proteome;S-adenosyl-L-methionine;Transferase SUBCELLULAR LOCATION: Nucleus Cytoplasm " Q9W4X9,"PROTEIN NAMES: Centromere/kinetochore protein zw10 (Mitotic 15 protein) PROTEIN FAMILY: ZW10 family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the ZW10 family. FUNCTION: Essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. Required for the assembly of the dynein-dynactin, Mad2 complexes and spindly/CG15415 onto kinetochores. During cytokinesis in male meiotic cells it is required for completion of cleavage furrow ingression, possibly in conjunction with Rint1. Required for maintenance of Golgi stack number and morphology, and acroblast assembly. Its function related to the spindle assembly machinery is proposed to depend on its association in the RZZ complex. Failure to assemble the complex due to the absence of any one of its components, results in the incorrect redistribution of the remaining components to diverse membrane compartments. KEYWORDS: Cell cycle;Cell division;Centromere;Chromosome;Cytoplasm;Cytoskeleton;Golgi apparatus;Kinetochore;Meiosis;Mitosis;Nucleus;Reference proteome SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Chromosome, centromere, kinetochore Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, spindle Golgi apparatus Golgi apparatus, Golgi stack Note=Dynamic pattern of localization during the cell cycle. Present in structures resembling Golgi stacks prior to their migration into the nuclear zone during prometaphase. At metaphase, detected at the kinetochores and kinetochore microtubules. During anaphase and telophase accumulates at the spindle envelope midzone and broad areas at the cell poles where they often become concentrated in small structures that resemble small Golgi-derived vesicles. In late telophase they also form compact aggregates at the interior of the equatorial region of the cell. " Q9W568,PROTEIN NAMES: Protein halfway (Protein singed wings) ORGANISM: Drosophila melanogaster (Fruit fly) FUNCTION: Has a role in the ecdysone induced cascade; probably indirect control of 'late' ecdysone genes. KEYWORDS: Glycoprotein;Leucine-rich repeat;Reference proteome;Repeat;Signal Q9W589,"PROTEIN NAMES: GDP-fucose protein O-fucosyltransferase 2 (Peptide-O-fucosyltransferase 2) (O-FucT-2) PROTEIN FAMILY: Glycosyltransferase 68 family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the glycosyltransferase 68 family. FUNCTION: Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue in the consensus sequence C1-X-X-S/T-C2 of thrombospondin type I repeats (TSRs) where C1 and C2 are the first and second cysteines of the repeat, respectively. O-fucosylates members of several protein families including the ADAMTS, the thrombospondin (TSP) and spondin families. PATHWAY: Protein modification; protein glycosylation. KEYWORDS: Carbohydrate metabolism;Disulfide bond;Endoplasmic reticulum;Fucose metabolism;Glycoprotein;Glycosyltransferase;Golgi apparatus;Reference proteome;Signal;Transferase SUBCELLULAR LOCATION: Endoplasmic reticulum Golgi apparatus Note=Mainly located in the endoplasmic reticulum. " Q9W5X9,"PROTEIN NAMES: Alpha-tubulin N-acetyltransferase 2 (Alpha-TAT 2) (TAT 2) (Acetyltransferase mec-17 homolog 2) (Protein leaky) PROTEIN FAMILY: Acetyltransferase ATAT1 family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the acetyltransferase ATAT1 family. FUNCTION: Specifically acetylates 'Lys-40' in alpha-tubulin on the lumenal side of microtubules. Promotes microtubule destabilization and accelerates microtubule dynamics; this activity may be independent of acetylation activity. Acetylates alpha-tubulin with a slow enzymatic rate, due to a catalytic site that is not optimized for acetyl transfer. Enters the microtubule through each end and diffuses quickly throughout the lumen of microtubules. Acetylates only long/old microtubules because of its slow acetylation rate since it does not have time to act on dynamically unstable microtubules before the enzyme is released (By similarity). Main acetyltransferase responsible for alpha-tubulin 'Lys-40' acetylation in germline cells during the early stages of oogenesis. Required for normal egg chamber separation. KEYWORDS: Acyltransferase;Reference proteome;Transferase SUBCELLULAR LOCATION: Midbody, Midbody ring Note=Localizes to fusomes in germline cells during oogenesis. MISCELLANEOUS: In the ovaries of mutant flies lacking expression of this protein the plasma membrane barrier between germline cells and surrounding somatic follicular cells is compromised, allowing cytoplasmic material to leak from one cell into the other, thus the name leaky." Q9W6K1,"PROTEIN NAMES: Double-strand break repair protein MRE11 PROTEIN FAMILY: MRE11/RAD32 family ORGANISM: Xenopus laevis (African clawed frog) SIMILARITY: Belongs to the MRE11/RAD32 family. FUNCTION: Core component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The MRN complex is involved in the repair of DNA double-strand breaks (DSBs) via homologous recombination (HR), an error-free mechanism which primarily occurs during S and G2 phases. The complex (1) mediates the end resection of damaged DNA, which generates proper single-stranded DNA, a key initial steps in HR, and is (2) required for the recruitment of other repair factors and efficient activation of ATM and ATR upon DNA damage (By similarity). Within the MRN complex, mre11 possesses both single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity (By similarity). After DSBs, mre11 is loaded onto DSBs sites and cleaves DNA by cooperating with rbbp8/CtIP to initiate end resection (By similarity). Mre11 first endonucleolytically cleaves the 5' strand at DNA DSB ends to prevent non-homologous end joining (NHEJ) and licence HR (By similarity). It then generates a single-stranded DNA gap via 3' to 5' exonucleolytic degradation to create entry sites for exo1- and dna2-mediated 5' to 3' long-range resection, which is required for single-strand invasion and recombination (By similarity). Rbbp8/CtIP specifically promotes the endonuclease activity of mre11 to clear protein-DNA adducts and generate clean double-strand break ends (By similarity). The MRN complex is also required for DNA damage signaling via activation of the atm and atr kinases: the nuclease activity of mre11 is not required to activate ATM and ATR (By similarity). The MRN complex promotes recruitment of topbp1 to DNA damage sites. The MRN complex and rbbp8/CtIP are also required for chromosome alignment during metaphase. KEYWORDS: Chromosome;DNA damage;DNA repair;Endonuclease;Exonuclease;Hydrolase;Manganese;Meiosis;Metal-binding;Nuclease;Nucleus;Reference proteome;Telomere SUBCELLULAR LOCATION: Nucleus Chromosome Chromosome, telomere Note=Localizes to DNA double-strand breaks (DSBs). " Q9WTK8,"PROTEIN NAMES: Meiotic recombination protein SPO11 PROTEIN FAMILY: TOP6A family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the TOP6A family. FUNCTION: [Isoform 1]: Component of a topoisomerase 6 complex specifically required for meiotic recombination. Together with TOP6BL, mediates DNA cleavage that forms the double-strand breaks (DSB) that initiate meiotic recombination. The complex promotes relaxation of negative and positive supercoiled DNA and DNA decatenation through cleavage and ligation cycles. Essential for the phosphorylation of SMC3, HORMAD1 and HORMAD2.; FUNCTION: [Isoform 4]: In contrast to isoform 1, does not mediate DNA cleavage that forms the double-strand breaks (DSB) that initiate meiotic recombination. KEYWORDS: Alternative splicing;DNA-binding;Isomerase;Magnesium;Meiosis;Metal-binding;Nucleus;Reference proteome;Topoisomerase SUBCELLULAR LOCATION: Nucleus " Q9WTN6,"PROTEIN NAMES: Solute carrier family 22 member 21 (Organic cation/carnitine transporter 3) (Solute carrier family 22 member 9) PROTEIN FAMILY: Major facilitator superfamily, Organic cation transporter family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the major facilitator (TC 2.A.1) superfamily. Organic cation transporter (TC 2.A.1.19) family. FUNCTION: Sodium-ion independent, medium affinity carnitine transporter. Also transports organic cations such as tetraethylammonium (TEA) without the involvement of sodium. Relative uptake activity ratio of carnitine to TEA is 746. KEYWORDS: ATP-binding;Glycoprotein;Ion transport;Membrane;Nucleotide-binding;Peroxisome;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Peroxisome membrane ; Multi-pass membrane protein " Q9WUP0,PROTEIN NAMES: Receptor activity-modifying protein 2 PROTEIN FAMILY: RAMP family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the RAMP family. FUNCTION: Transports the calcitonin gene-related peptide type 1 receptor (CALCRL) to the plasma membrane. Acts as a receptor for adrenomedullin (AM) together with CALCRL. KEYWORDS: Disulfide bond;Glycoprotein;Membrane;Receptor;Reference proteome;Signal;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Membrane ; Single-pass type I membrane protein Q9WVG5,"PROTEIN NAMES: Endothelial lipase (Endothelial cell-derived lipase) (EDL) (Phospholipase A1) PROTEIN FAMILY: AB hydrolase superfamily, Lipase family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the AB hydrolase superfamily. Lipase family. FUNCTION: Exerts both phospholipase and triglyceride lipase activities (By similarity). More active as a phospholipase than a triglyceride lipase (By similarity). Hydrolyzes triglycerides, both with short-chain fatty acyl groups (tributyrin) and long-chain fatty acyl groups (triolein) with similar levels of activity toward both types of substrates (By similarity). Hydrolyzes high density lipoproteins (HDL) more efficiently than other lipoproteins (By similarity). KEYWORDS: Disulfide bond;Glycoprotein;Heparin-binding;Hydrolase;Lipid degradation;Lipid metabolism;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted " Q9WVH6,"PROTEIN NAMES: Angiopoietin-4 (ANG-4) (Angiopoietin-3) (ANG-3) ORGANISM: Mus musculus (Mouse) FUNCTION: Binds to TEK/TIE2, modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2. Promotes endothelial cell survival, migration and angiogenesis. KEYWORDS: Angiogenesis;Coiled coil;Disulfide bond;Glycoprotein;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted " Q9WVR1,"PROTEIN NAMES: Phosphatidylinositol polyphosphate 5-phosphatase type IV (5-phosphatase that induces arborization) (Pharbin) (72 kDa inositol polyphosphate 5-phosphatase) (Inositol polyphosphate-5-phosphatase E) (Phosphatidylinositol 4,5-bisphosphate 5-phosphatase) (Phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase) PROTEIN FAMILY: Inositol 1,4,5-trisphosphate 5-phosphatase type IV family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the inositol 1,4,5-trisphosphate 5-phosphatase type IV family. FUNCTION: Phosphatidylinositol (PtdIns) phosphatase that specifically hydrolyzes the 5-phosphate of phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3), phosphatidylinositol 4,5-bisphosphate PtdIns (4,5)P2 and phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Specific for lipid substrates, inactive towards water soluble inositol phosphates (By similarity). Plays an essential role in the primary cilium by controlling ciliary growth and phosphoinositide 3-kinase (PI3K) signaling and stability (By similarity). KEYWORDS: Cell membrane;Cell projection;Cilium;Cytoplasm;Cytoskeleton;Golgi apparatus;Hydrolase;Lipid metabolism;Lipoprotein;Membrane;Methylation;Nucleus;Phosphoprotein;Prenylation;Reference proteome;Repeat SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, cilium axoneme Golgi apparatus, Golgi stack membrane ; Peripheral membrane protein ; Cytoplasmic side Cell membrane ; Peripheral membrane protein ; Cytoplasmic side Cell projection, ruffle Cytoplasm Nucleus Note=Peripheral membrane protein associated with Golgi stacks. " Q9WXT2,"PROTEIN NAMES: Cephalosporin-C deacetylase (Acetylxylan esterase) PROTEIN FAMILY: Carbohydrate esterase 7 family ORGANISM: Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) SIMILARITY: Belongs to the carbohydrate esterase 7 family. FUNCTION: Esterase that removes acetyl groups from a number of O-acetylated small substrates, such as acetylated xylose, short xylo-oligosaccharides and cephalosporin C. Has no activity towards polymeric acetylated xylan, 4-methylumbelliferyl acetate or alpha-naphthyl acetate. Able to catalyze rapid hydrolysis of a range of substrates preferably with acetate groups, independent of the alcohol moiety. Exhibits a narrow selectivity for short chain acyl esters (C2-C3). Displays broad substrate specificity by hydrolyzing acetate at 2, 3, and 4 positions of 4-nitrophenyl-beta-D-xylopyranoside (pNP-Xyl) with similar efficiency. Cannot cleave amide linkages. KEYWORDS: 3D-structure;Calcium;Carbohydrate metabolism;Cellulose degradation;Cytoplasm;Hydrolase;Metal-binding;Polysaccharide degradation;Reference proteome;Serine esterase SUBCELLULAR LOCATION: Cytoplasm " Q9WYH1,"PROTEIN NAMES: Esterase EstD PROTEIN FAMILY: AB hydrolase superfamily, Esterase 10 family ORGANISM: Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) SIMILARITY: Belongs to the AB hydrolase superfamily. Esterase 10 family. FUNCTION: Exhibits significant esterase activity with a preference for short acyl chain esters (C4-C8) in vitro. Its physiological function is not known. Displays neither proteolytic activity using casein as substrate, nor peptidase activity when assayed with L-leucine p-nitroanilide and L-proline p-nitroanilide. KEYWORDS: Hydrolase;Reference proteome;Serine esterase;Signal " Q9WYR4,"PROTEIN NAMES: Pectate trisaccharide-lyase (Pectate lyase A) (PelA) PROTEIN FAMILY: Polysaccharide lyase 1 family ORGANISM: Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) SIMILARITY: Belongs to the polysaccharide lyase 1 family. FUNCTION: Cleaves unsaturated trigalacturonate from pectin. Activity is highest towards polygalacturonic acid, activity on methylated pectins decreases with an increasing degree of methylation. KEYWORDS: 3D-structure;Calcium;Carbohydrate metabolism;Cell wall biogenesis/degradation;Lyase;Metal-binding;Polysaccharide degradation;Reference proteome;Repeat;Secreted;Signal SUBCELLULAR LOCATION: Secreted " Q9WYW0,"PROTEIN NAMES: NAD-dependent protein deacetylase (Regulatory protein SIR2 homolog) (Sir2Tm) PROTEIN FAMILY: Sirtuin family, Class U subfamily ORGANISM: Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) SIMILARITY: Belongs to the sirtuin family. Class U subfamily. FUNCTION: NAD-dependent protein deacetylase which modulates the activities of several enzymes which are inactive in their acetylated form. Has also depropionylation activity in vitro. Also able to ADP-ribosylate peptide substrates with Arg or Lys in the +2 position. The role of this function in vivo is not clear. KEYWORDS: 3D-structure;Cytoplasm;Metal-binding;NAD;Reference proteome;Transferase;Zinc SUBCELLULAR LOCATION: Cytoplasm " Q9WZP7,"PROTEIN NAMES: Bifunctional thiamine biosynthesis protein ThiDN [Includes: Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase (Hydroxymethylpyrimidine kinase) (HMP kinase) (Hydroxymethylpyrimidine phosphate kinase) (HMP-P kinase) (HMP-phosphate kinase) (HMPP kinase); Thiamine-phosphate synthase ThiN (TP synthase) (TPS) (Thiamine-phosphate pyrophosphorylase) (TMP pyrophosphorylase) (TMP-PPase)] PROTEIN FAMILY: ThiD family; ThiN family ORGANISM: Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) SIMILARITY: In the N-terminal section; belongs to the ThiD family.; SIMILARITY: In the C-terminal section; belongs to the ThiN family. FUNCTION: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P.; FUNCTION: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP). PATHWAY: Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-amino-2-methyl-5-diphosphomethylpyrimidine from 5-amino-1-(5-phospho-D-ribosyl)imidazole.; PATHWAY: Cofactor biosynthesis; thiamine diphosphate biosynthesis; thiamine phosphate from 4-amino-2-methyl-5-diphosphomethylpyrimidine and 4-methyl-5-(2-phosphoethyl)-thiazole: step 1/1. KEYWORDS: ATP-binding;Kinase;Multifunctional enzyme;Nucleotide-binding;Reference proteome;Thiamine biosynthesis;Transferase " Q9X0X3,"PROTEIN NAMES: Phosphoribosylformylglycinamidine synthase subunit PurL (FGAM synthase) (Formylglycinamide ribonucleotide amidotransferase subunit II) (FGAR amidotransferase II) (FGAR-AT II) (Glutamine amidotransferase PurL) (Phosphoribosylformylglycinamidine synthase subunit II) PROTEIN FAMILY: FGAMS family ORGANISM: Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) SIMILARITY: Belongs to the FGAMS family. FUNCTION: Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL. PATHWAY: Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. KEYWORDS: 3D-structure;ATP-binding;Cytoplasm;Ligase;Magnesium;Metal-binding;Nucleotide-binding;Purine biosynthesis;Reference proteome SUBCELLULAR LOCATION: Cytoplasm MISCELLANEOUS: Purl possesses an auxiliary ATP Binding region which is not catalytic, but able to bind additional ATP. The conformational changes associated with its binding could have a regulatory role in formation of the PurLSQ complex." Q9X1N5,"PROTEIN NAMES: Undecaprenyl-phosphate alpha-N-acetylglucosaminyl 1-phosphate transferase (UDP-GlcNAc:undecaprenyl-phosphate GlcNAc-1-phosphate transferase) (Undecaprenyl-phosphate GlcNAc-1-phosphate transferase) PROTEIN FAMILY: Glycosyltransferase 4 family, WecA subfamily ORGANISM: Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) SIMILARITY: Belongs to the glycosyltransferase 4 family. WecA subfamily. FUNCTION: Catalyzes the transfer of the GlcNAc-1-phosphate moiety from UDP-GlcNAc onto the carrier lipid undecaprenyl phosphate (C55-P), yielding GlcNAc-pyrophosphoryl-undecaprenyl (GlcNAc-PP-C55), the lipid intermediate involved in the synthesis of various bacterial cell envelope components. The enzyme is highly active when tested with C35-P, instead of its natural C55-P lipid substrate, suggesting that at least a 35-carbon chain is required for the lipid to be a substrate of WecA. PATHWAY: Cell wall biogenesis; cell wall polysaccharide biosynthesis. KEYWORDS: Cell membrane;Glycosyltransferase;Magnesium;Manganese;Membrane;Reference proteome;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein " Q9X1Z1,"PROTEIN NAMES: Energy-coupling factor transporter ATP-binding protein EcfA1 PROTEIN FAMILY: ABC transporter superfamily, Energy-coupling factor EcfA family ORGANISM: Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) SIMILARITY: Belongs to the ABC transporter superfamily. Energy-coupling factor EcfA family. FUNCTION: ATP-binding (A) component of a common energy-coupling factor (ECF) ABC-transporter complex. Unlike classic ABC transporters this ECF transporter provides the energy necessary to transport a number of different substrates (Probable). Expression of the complex plus RibU in E.coli allows riboflavin uptake; uptake does not occur in the absence of RibU or the EcfA1A2T complex. KEYWORDS: 3D-structure;ATP-binding;Cell inner membrane;Cell membrane;Membrane;Nucleotide-binding;Reference proteome;Translocase;Transport SUBCELLULAR LOCATION: Cell inner membrane ; Peripheral membrane protein MISCELLANEOUS: Structure 4HLU is probably in the open state." Q9X5T6,"PROTEIN NAMES: Mitomycin biosynthesis 6-O-methyltransferase (Mitomycin 7-O-methyltransferase) PROTEIN FAMILY: Class I-like SAM-binding methyltransferase superfamily, Cation-independent O-methyltransferase family, COMT subfamily ORGANISM: Streptomyces lavendulae SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O-methyltransferase family. COMT subfamily. FUNCTION: Involved in the biosynthesis of the quinone methoxy group present in the mitomycin A and B, which are used as anticancer agents. In vitro, catalyzes the 6-O-methylation of both C9-beta- and C9-alpha-configured 6-hydroxymitomycins via the transfer of the S-methyl group of S-adenosyl-L-methionine (AdoMet) to the 6-demethylmitomycin A and B. It can also use hydroxyquinone as substrate. KEYWORDS: 3D-structure;Methyltransferase;S-adenosyl-L-methionine;Transferase " Q9XB60,"PROTEIN NAMES: Carboxymethylproline synthase (Carbapenem biosynthesis protein B) PROTEIN FAMILY: Enoyl-CoA hydratase/isomerase family ORGANISM: Pectobacterium carotovorum subsp. carotovorum (Erwinia carotovora subsp. carotovora) SIMILARITY: Belongs to the enoyl-CoA hydratase/isomerase family. FUNCTION: Catalyzes the formation of (2S,5S)-carboxymethylproline (t-CMP) from malonyl-CoA and (S)-1-pyrroline-5-carboxylate, the first step in the biosynthesis of (5R)-carbapen-2-em-3-carboxylate, a beta-lactam antibiotic of the carbapenem class. Also catalyzes the independent decarboxylation of malonyl-CoA and methylmalonyl-CoA and the hydrolysis of CoA esters such as acetyl-CoA and propionyl-CoA. Catalyzes the reaction with a C2 epimeric mixture of methylmalonyl-CoA to give a 55:45 mixture of (6R)- and (6S)-epimers of 6-methyl-t-CMP, under standard incubation conditions. PATHWAY: Antibiotic biosynthesis; carbapenem biosynthesis. KEYWORDS: 3D-structure;Antibiotic biosynthesis;Transferase MISCELLANEOUS: Unusual member of the enoyl-CoA hydratase/isomerase family: in addition to decarboxylation and thioester hydrolysis steps, catalyzes C-C bond formation leading to a substituted heterocycle. Glu-131 is important in both C-C bond formation and thioester hydrolysis steps while it does not play an essential role in decarboxylation." Q9XDM4,"PROTEIN NAMES: L-threonine kinase (Propanediol utilization protein PduX) PROTEIN FAMILY: GHMP kinase family, PduX subfamily ORGANISM: Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) SIMILARITY: Belongs to the GHMP kinase family. PduX subfamily. FUNCTION: L-threonine kinase that catalyzes the conversion of L-threonine to L-threonine-O-3-phosphate. Involved in the de novo synthesis of adenosylcobalamin (coenzyme B12) and the assimilation of cobyric acid. Uses ATP; the activity with CTP, GTP or UTP is 6, 11, and 3% of the activity with ATP, respectively.; FUNCTION: The 1,2-propanediol (1,2-PD)-specific bacterial microcompartment (BMC) concentrates low levels of 1,2-PD catabolic enzymes, concentrates volatile reaction intermediates thus enhancing pathway flux and keeps the level of toxic, mutagenic propionaldehyde low. This gene probably benefits from its induction via the Pdu promoter, rather than a physical interaction with the BMC. PATHWAY: Cofactor biosynthesis; adenosylcobalamin biosynthesis.; PATHWAY: Polyol metabolism; 1,2-propanediol degradation. KEYWORDS: ATP-binding;Cobalamin biosynthesis;Cytoplasm;Kinase;Nucleotide-binding;Reference proteome;Transferase SUBCELLULAR LOCATION: Cytoplasm MISCELLANEOUS: Catalysis proceeds by a steady-state ordered bi-bi complex mechanism in which ATP is the first substrate to bind." Q9XEE9,"PROTEIN NAMES: GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase (Alpha-1,2-mannosyltransferase ALG11) (Asparagine-linked glycosylation protein 11) (Protein LEAF WILTING 3) PROTEIN FAMILY: Glycosyltransferase 4 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the glycosyltransferase 4 family. FUNCTION: GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. Catalyzes, on the cytoplasmic face of the endoplasmic reticulum, the addition of the fourth and fifth mannose residues to the dolichol-linked oligosaccharide chain, to produce Man(5)GlcNAc(2)-PP-dolichol core oligosaccharide. Man(5)GlcNAc(2)-PP-dolichol is a substrate for ALG3, the following enzyme in the biosynthetic pathway. PATHWAY: Protein modification; protein glycosylation. KEYWORDS: Alternative splicing;Endoplasmic reticulum;Glycosyltransferase;Membrane;Reference proteome;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Single-pass membrane protein MISCELLANEOUS: In the absence of ALG11 activity, no N-glycans are produced and transferred to proteins." Q9XF67,"PROTEIN NAMES: 3-phosphoinositide-dependent protein kinase 1 (AtPDK1) PROTEIN FAMILY: Protein kinase superfamily, AGC Ser/Thr protein kinase family, PDPK1 subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDPK1 subfamily. FUNCTION: May couple lipid signals to the activation-loop phosphorylation of several protein kinases of the so-called AGC kinase family. Interacts via its pleckstrin homology domain with phosphatidic acid, PtdIns3P and PtdIns(3,4)P2 and to a lesser extent with PtdIns(4,5)P2 and PtdIns4P. May play a general role in signaling processes controlling the pathogen/stress response, polar auxin transport and development. Transphosphorylates the AGC protein kinases OXI1/AGC2-1, PK1/S6K1, PK19/S6K2 and PID resulting in their activation. KEYWORDS: Alternative splicing;ATP-binding;Cytoplasm;Kinase;Membrane;Nucleotide-binding;Phosphoprotein;Reference proteome;Serine/threonine-protein kinase;Transferase SUBCELLULAR LOCATION: Cytoplasm Membrane ; Peripheral membrane protein Note=Membrane-associated after cell stimulation. " Q9XF89,"PROTEIN NAMES: Chlorophyll a-b binding protein CP26, chloroplastic (LHCB5) (LHCIIc) (Light-harvesting complex II protein 5) PROTEIN FAMILY: Light-harvesting chlorophyll a/b-binding (LHC) protein family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the light-harvesting chlorophyll a/b-binding (LHC) protein family. FUNCTION: The light-harvesting complex (LHC) functions as a light receptor, it captures and delivers excitation energy to photosystems with which it is closely associated. KEYWORDS: 3D-structure;Chlorophyll;Chloroplast;Chromophore;Magnesium;Membrane;Metal-binding;Phosphoprotein;Photosynthesis;Photosystem I;Photosystem II;Plastid;Reference proteome;Thylakoid;Transit peptide;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane ; Multi-pass membrane protein. " Q9XFM0,"PROTEIN NAMES: Auxin-responsive protein IAA28 (Indoleacetic acid-induced protein 28) PROTEIN FAMILY: Aux/IAA family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the Aux/IAA family. FUNCTION: Aux/IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations. Repression is thought to result from the interaction with auxin response factors (ARFs), proteins that bind to the auxin-responsive promoter element (AuxRE). Formation of heterodimers with ARF proteins may alter their ability to modulate early auxin response genes expression. KEYWORDS: Auxin signaling pathway;Nucleus;Reference proteome;Repressor;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " Q9XGM8,"PROTEIN NAMES: Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase (N-acetylglucosaminyltransferase I) (GlcNAcT-I) (N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase I) (Protein COMPLEX GLYCAN LESS 1) PROTEIN FAMILY: Glycosyltransferase 13 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the glycosyltransferase 13 family. FUNCTION: Initiates complex N-linked carbohydrate formation. Essential for the conversion of high-mannose to hybrid and complex N-glycans. Required for normal root growth and morphology. PATHWAY: Protein modification; protein glycosylation. KEYWORDS: Alternative splicing;Coiled coil;Glycoprotein;Glycosyltransferase;Golgi apparatus;Manganese;Membrane;Metal-binding;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus membrane ; Single-pass type II membrane protein MISCELLANEOUS: Creation of a second N-glycosylation site in mutant cgl1 C5/cgl1-1 interferes with protein folding and sequesters the protein for degradation in the endoplasmic reticulum." Q9XI19,"PROTEIN NAMES: PLASTID TRANSCRIPTIONALLY ACTIVE protein 6, chloroplastic (pTAC6) (Plastid-encoded RNA polymerase-associated protein 8) (PEP-associated protein 8) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Essential protein involved in plastid gene expression and in chloroplast biogenesis. Links photomorphogenesis and chloroplast biogenesis through its dual localization; required for the formation of late photobodies in the nucleus, as well as for phytochrome B-mediated signaling cascade and subsequent reshaping of the plastid-encoded RNA polymerase (PEP) activity. Binds RNA via specific recognition motifs of viral origin. Recruited by MTERF5 to the transcriptionally paused region of psbEFLJ. Promotes leaf greening. KEYWORDS: Chloroplast;Nucleus;Plastid;Reference proteome;RNA-binding;Thylakoid;Transcription;Transcription regulation;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast Plastid, chloroplast thylakoid Nucleus Nucleus, nucleoplasm Note=Translocates to the chloroplast via its transit peptide and then take part of the plastid-encoded RNA polymerase (PEP) complex. When in the nucleus, can bind to PTAC12/HMR/PAP5. Both localization are observed in the dark or under white light, mainly present in the nucleus of etiolated seedlings while enriched in chloroplast after light exposure. " Q9XI47,"PROTEIN NAMES: Protein SAWADEE HOMEODOMAIN HOMOLOG 1 (DNA-binding transcription factor 1) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Involved in RNA-directed DNA methylation (RdDM). Required for the silencing of some endogenous RdDM targets and accumulation of 24-nt siRNAs, but not for the production of Pol V-dependent transcripts. Functions in transcriptional silencing through both DNA methylation-dependent and -independent pathways. Required for both maintenance and de-novo DNA methylation. Plays a role in the recruitment of Pol IV to genomic regions associated with K9 methylated histone H3 that are targets for RdDM. KEYWORDS: 3D-structure;Alternative splicing;Metal-binding;Nucleus;Reference proteome;Zinc SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: Associates in vivo with Pol IV but not with Pol V and this interaction is not dependent on its H3K9me binding activity. Glu-130 and Asp-141 interact with the H3K4 side chain while the H3K9me1/2/3 side chains insert into a hydrophobic aromatic cage formed by Tyr-140, Phe-162 and Phe-165." Q9XI90,"PROTEIN NAMES: Probable WRKY transcription factor 4 (WRKY DNA-binding protein 4) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Transcription factor that binds specifically to the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element. Has a positive role in resistance to necrotrophic pathogens (e.g. Botrytis cinerea), but a negative effect on plant resistance to biotrophic pathogens (e.g. Pseudomonas syringae). KEYWORDS: 3D-structure;Alternative splicing;DNA-binding;Metal-binding;Nucleus;Plant defense;Reference proteome;Repeat;Transcription;Transcription regulation;Zinc SUBCELLULAR LOCATION: Nucleus " Q9XIN7,"PROTEIN NAMES: NAC domain-containing protein 40 (ANAC040) (Protein NTM1-like 8) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Transcriptional activator activated by proteolytic cleavage through regulated intramembrane proteolysis (RIP), probably via metalloprotease activity. Regulates gibberellic acid-mediated salt-responsive repression of seed germination and flowering via FT, thus delaying seed germination under high salinity conditions. KEYWORDS: Activator;Cell membrane;DNA-binding;Flowering;Gibberellin signaling pathway;Membrane;Nucleus;Reference proteome;Stress response;Transcription;Transcription regulation;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass membrane protein Nucleus Note=Localized primarily in plasma membrane as dormant form and, upon salt stress, is processed into a transcriptionally active and nuclear form after a proteolytic cleavage through regulated intramembrane proteolysis (RIP). " Q9XIP2,"PROTEIN NAMES: OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 6 (OTU domain-containing protein 6) (Deubiquitinating enzyme OTU6) (Otubain-like deubiquitinase 1) PROTEIN FAMILY: Peptidase C85 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the peptidase C85 family. FUNCTION: Hydrolase that can remove conjugated ubiquitin from proteins in vitro and may therefore play an important regulatory role at the level of protein turnover by preventing degradation. Binds chromatin (e.g. nucleosomes and histones) and has enzymatic histone deubiquitinase activity, specific for the H2B histone. Can both repress (e.g. OSR2) and promote (e.g. AN3) the expression of target genes by associating with chromatin, deubiquitinating H2B and regulating its euchromatic histone marks (e.g. H3ac and H3K4me). In association with LDL1/KDM1C, involved in transcriptional gene repression via histone deubiquitination and demethylation. Promotes the concerted epigenetic regulation and repression (e.g. the removal of euchromatic histone acetylation, ubiquitination, and methylation marks) of a set of genes (e.g. GA20OX, WUS, OSR2, ARL and ABI5) that collectively limit plant growth thus stimulating plant growth and increasing cell size. KEYWORDS: Activator;Alternative splicing;Chromatin regulator;Cytoplasm;Hydrolase;Nucleus;Protease;Reference proteome;Repressor;Ubl conjugation pathway SUBCELLULAR LOCATION: Nucleus Cytoplasm " Q9XJ35,"PROTEIN NAMES: ATP-dependent Clp protease proteolytic subunit-related protein 1, chloroplastic (ClpR1) (Protein SUPPRESSOR OF VARIEGATION 2) (nClpP5) PROTEIN FAMILY: Peptidase S14 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the peptidase S14 family. FUNCTION: Required for chloroplast development and differentiation. KEYWORDS: Chloroplast;Direct protein sequencing;Plastid;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast stroma " Q9XJ36,"PROTEIN NAMES: ATP-dependent Clp protease proteolytic subunit-related protein 2, chloroplastic (ClpR2) (nClpP2) PROTEIN FAMILY: Peptidase S14 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the peptidase S14 family. FUNCTION: Required for chloroplast development and integrity. Involved in the regulation of plastoglobules formation. KEYWORDS: Chloroplast;Direct protein sequencing;Plastid;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast " Q9XTG1,PROTEIN NAMES: Neuroligin-1 PROTEIN FAMILY: Type-B carboxylesterase/lipase family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the type-B carboxylesterase/lipase family. FUNCTION: Probable neuronal cell surface protein thought to be involved in cell-cell-interactions by forming intercellular junctions through binding to beta-neurexins (By similarity). Plays a role in the clustering of the GABA(A) receptor unc-49 at postsynaptic sites in neuromuscular junctions (NMJs) via the interaction with madd-4 and neurexin nrx-1 and is thereby required for normal GABAergic synaptic transmission. KEYWORDS: Alternative splicing;Cell adhesion;Cell membrane;Disulfide bond;Glycoprotein;Membrane;Reference proteome;Signal;Synapse;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein Synapse Note=Localizes specifically to GABAergic synapses. Q9XTQ6,"PROTEIN NAMES: Tyramine beta-hydroxylase (Tyramine beta-monooxygenase) (TbetaM) PROTEIN FAMILY: Copper type II ascorbate-dependent monooxygenase family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the copper type II ascorbate-dependent monooxygenase family. FUNCTION: Required for the conversion of tyramine to octopamine, a precursor of octapamine but probably itself a neurotransmitter. Involved in the regulation of egg laying, which is inhibited by tyramine. Due to its involvement in octopamine biosynthesis, also required for crtc-1-dependent regulation of AMPK-mediated longevity. KEYWORDS: Alternative splicing;Copper;Disulfide bond;Glycoprotein;Membrane;Metal-binding;Monooxygenase;Neurotransmitter biosynthesis;Oxidoreductase;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: [Isoform b]: Membrane ; Single-pass membrane protein " Q9XTR1,"PROTEIN NAMES: Cyclin-dependent kinase 4 homolog (CDK4/6) (Cell division protein kinase 4) PROTEIN FAMILY: Protein kinase superfamily, CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. FUNCTION: Serine/threonine-protein kinase which, in association with cyclin D-like protein cyd-1, is required for the progression through the G1 phase of the cell cycle during postembryonic development by phosphorylating and inhibiting lin-35 and fzr-1. In complex with cyd-1, involved in sex determination during gonadogenesis by regulating the asymmetric division of the somatic gonadal precursor cell (SGP). KEYWORDS: Alternative splicing;ATP-binding;Cell cycle;Cell division;Kinase;Magnesium;Metal-binding;Nucleotide-binding;Reference proteome;Serine/threonine-protein kinase;Transferase " Q9XTY6,"PROTEIN NAMES: Numb-related protein 1 (CKA1) (Protein kinase C adapter 1) ORGANISM: Caenorhabditis elegans FUNCTION: Involved in the tethering and targeting of pkc-3 to modulate the intracellular distribution of the kinase. The complex formed with pkc-3 complexes are likely to be involved in assembly, maintenance, and/or regulation of protein complexes that execute asymmetric and/or polarized cell functions. KEYWORDS: Alternative initiation;Alternative splicing;Cytoplasm;Cytoskeleton;Developmental protein;Membrane;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cytoplasm, cell cortex. Cytoplasm, cytoskeleton. Membrane; Peripheral membrane protein. Note=Expressed at the inner surface of the plasma membrane at the cell periphery (which includes a region corresponding to plasma membrane and/or actin cortical cytoskeleton) in early embryos. Tightly associated with organelles and/or cytoskeletal structures with some diffuse expression in the cytoplasm. Differentially routed to lateral junctions between polarized cells. MISCELLANEOUS: [Isoform b]: Produced by alternative splicing.; MISCELLANEOUS: [Isoform c]: Produced by alternative initiation at Met-45 of isoform a." Q9XU42,PROTEIN NAMES: Receptor-type guanylate cyclase gcy-27 PROTEIN FAMILY: Adenylyl cyclase class-4/guanylyl cyclase family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the adenylyl cyclase class-4/guanylyl cyclase family. FUNCTION: Guanylate cyclase involved in the production of the second messenger cGMP (By similarity). May be involved in sensitivity to quinine by regulating egl-4 activity through the production of cGMP. Promotes the calcium flux to the cytoplasm in ASJ sensory neurons upon removal of a nitric oxide (NO) stimulus and is thereby involved in the behavioral avoidance response to NO-producing organisms like P.aeruginosa. KEYWORDS: Alternative splicing;Cell membrane;cGMP biosynthesis;Glycoprotein;GTP-binding;Lyase;Membrane;Nucleotide-binding;Reference proteome;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein Q9XU75,"PROTEIN NAMES: Putative carboxypeptidase suro-1 (Suppressor of roller 1) PROTEIN FAMILY: Peptidase M14 family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the peptidase M14 family. FUNCTION: May play a role in processing or organization of cuticle collagen proteins, including rol-6 and col-19. KEYWORDS: Carboxypeptidase;Cytoplasmic vesicle;Disulfide bond;Hydrolase;Metal-binding;Metalloprotease;Protease;Reference proteome;Secreted;Signal;Zinc;Zymogen SUBCELLULAR LOCATION: Cytoplasmic vesicle Secreted " Q9XUB2,"PROTEIN NAMES: Zinc finger protein mex-5 ORGANISM: Caenorhabditis elegans FUNCTION: Functions with mex-6 to affect embryonic viability, establish soma germline asymmetry in embryos and establish plk-1, pie-1, mex-1, and pos-1 asymmetry in embryos. Also affects formation of intestinal cells. Binds to mRNA in vitro, and inhibits pgl-3-mediated P-granule formation, probably by competing with pgl-3 for binding to mRNA. Required for neg-1 expression in anterior blastomeres during embryogenesis. KEYWORDS: 3D-structure;Cytoplasm;Developmental protein;DNA-binding;Metal-binding;Phosphoprotein;Reference proteome;Repeat;RNA-binding;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cytoplasm " Q9XUC2,"PROTEIN NAMES: Intraflagellar transport associated protein 2 PROTEIN FAMILY: Small GTPase superfamily, Rab family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the small GTPase superfamily. Rab family. FUNCTION: Component of the intraflagellar transport (IFT) complex B required for transport of proteins in the motile cilium. May be required for ciliary entrance and transport of specific ciliary cargo proteins such as che-3 which are related to motility. Regulates specific signaling activities in the cilia, such as the daf-2/insulin receptor-like transduction pathway. KEYWORDS: Cell projection;Cilium;Cytoplasm;Cytoskeleton;GTP-binding;Nucleotide-binding;Reference proteome SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, cilium axoneme Note=Moves along the axoneme. " Q9XUC4,"PROTEIN NAMES: Zinc transporter zipt-7.1 (Histidine-rich membrane protein KE4 homolog 1) PROTEIN FAMILY: ZIP transporter family, KE4/Catsup subfamily ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the ZIP transporter (TC 2.A.5) family. KE4/Catsup subfamily. FUNCTION: Zinc transporter which regulates intracellular zinc levels. Required for spermatogenesis in both hermaphrodites and males where it resides in an inactive form in immature sperm, spermatids, but is likely activated in response to reduced spe-4 and spe-6 function. Upon activation, mediates the release of zinc from internal stores in spermatids into the cytoplasm. The resulting increase in cytoplasmic zinc levels promotes spermatid activation and subsequent differentiation into mature motile sperm that are capable of fertilization. KEYWORDS: Differentiation;Glycoprotein;Ion transport;Membrane;Reference proteome;Spermatogenesis;Transmembrane;Transmembrane helix;Transport;Zinc;Zinc transport SUBCELLULAR LOCATION: Membrane ; Multi-pass membrane protein Note=Localizes to membranous organelles. " Q9XWF0,"PROTEIN NAMES: 3beta-hydroxysteroid dehydrogenase/Delta(5)-Delta(4) isomerase 1 (HSD-1) (3beta_HSD domain-containing protein 1) PROTEIN FAMILY: 3-beta-HSD family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the 3-beta-HSD family. FUNCTION: Hydroxysteroid dehydrogenase involved in the biosynthesis of dafrachonic acids. Catalyzes the dehydrogenation of cholesterol or its derivatives and the isomerization of the double carbon bond on the sterol ring. Modifies sterols into a Delta(4)-3-keto-sterols such as cholest-4-en-3-one, precursor of Delta(4)-dafachronic acid. Contributes to the production of Delta(7)-dafachronic acid in the XXX cells. Dafachronic acids act as ligands and bind directly to the nuclear hormone receptor (NHR) daf-12 suppressing dauer formation and inducing reproductive growth. Acts in parallel to AKT-1 to promote reproductive development via DAF-16/FoxO and DAF-12. PATHWAY: Steroid hormone biosynthesis; dafachronic acid biosynthesis. KEYWORDS: Cholesterol metabolism;Isomerase;Lipid metabolism;Membrane;NAD;Oxidoreductase;Reference proteome;Steroid metabolism;Sterol metabolism;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Multi-pass membrane protein " Q9XWN9,"PROTEIN NAMES: Doublesex- and mab-3-related transcription factor dmd-3 (Doublesex-like 3 protein homolog) PROTEIN FAMILY: DMRT family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the DMRT family. FUNCTION: Transcriptional activator which promotes male-specific development. Acts partially redundantly with the transcription factor mab-3 to coordinate tail tip cell fusion and retraction and thereby regulate male tail tip morphogenesis. This is most likely through the regulation of downstream effectors such as eff-1. May also negatively regulate the expression of other proteins implicated in male tail morphogenesis including nhr-25, vav-1 and arl-1 in tail tip cells. In males, plays a role in the development of ray A-neurons by negatively regulating the activity of the transcription factor ast-1. Plays a role in the male-specific differentiation of PHC sensory neurons into densely connected hub sensory neurons. Plays a role in male mating behavior. KEYWORDS: Alternative splicing;Developmental protein;Differentiation;DNA-binding;Metal-binding;Nucleus;Reference proteome;Repeat;Sexual differentiation;Transcription;Transcription regulation;Zinc SUBCELLULAR LOCATION: Nucleus Perikaryon " Q9XWP6,"PROTEIN NAMES: Lysine-specific histone demethylase 1 (P110b homolog) (Suppressor of presenilin 5) PROTEIN FAMILY: Flavin monoamine oxidase family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the flavin monoamine oxidase family. FUNCTION: Histone demethylase that specifically demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Demethylates both mono- and di-methylated 'Lys-4' of histone H3 (By similarity). May be involved in H3 demethylation in mitotic cells including gut and embryonic cells. Participates in the transcriptional repression of the presenilin protein hop-1. May act via the formation of a multiprotein complex that remodel or modify the chromatin. Together with met-2, set-17 and set-26, required for transgenerational fertility. Plays a role in developmental growth and lifespan regulation in response to ultraviolet-induced damage. KEYWORDS: Chromatin regulator;FAD;Flavoprotein;Nucleus;Oxidoreductase;Reference proteome;Repressor;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " Q9XXV0,"PROTEIN NAMES: Phenoloxidase-activating enzyme (Prophenoloxidase-activating enzyme) [Cleaved into: Phenoloxidase-activating enzyme light chain; Phenoloxidase-activating enzyme heavy chain] PROTEIN FAMILY: Peptidase S1 family, CLIP subfamily ORGANISM: Bombyx mori (Silk moth) SIMILARITY: Belongs to the peptidase S1 family. CLIP subfamily. FUNCTION: Endopeptidase with selective post-Arg cleavage site. Activates prophenoloxidase. Has a probable role in the melanization process as part of the innate immune response (Probable). KEYWORDS: Calcium;Direct protein sequencing;Disulfide bond;Glycoprotein;Hydrolase;Metal-binding;Protease;Pyrrolidone carboxylic acid;Reference proteome;Serine protease;Signal;Zymogen " Q9XYD3,PROTEIN NAMES: Hunchback-like protein PROTEIN FAMILY: Hunchback C2H2-type zinc-finger protein family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the hunchback C2H2-type zinc-finger protein family. FUNCTION: Required for the late stages of development. Plays a role in the developmental timing of postembryonic hypodermal seam cell fusion events and adult alae production. KEYWORDS: Developmental protein;DNA-binding;Metal-binding;Nucleus;Reference proteome;Repeat;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Q9XYF4,PROTEIN NAMES: Caspase Dronc (NEDD2-like caspase) [Cleaved into: Caspase Nc subunit 1; Caspase Nc subunit 2] PROTEIN FAMILY: Peptidase C14A family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the peptidase C14A family. FUNCTION: Involved in the activation cascade of caspases responsible for apoptosis execution. Effector of steroid-mediated apoptosis during insect metamorphosis. Overexpression promotes programmed cell death. Interaction with Diap1 is required to suppress Dronc-mediated cell death; via Diap1-mediated ubiquitination of Dronc. Rate-limiting caspase in rpr and hid death pathway. KEYWORDS: 3D-structure;Apoptosis;Cytoplasm;Hydrolase;Protease;Reference proteome;Thiol protease;Ubl conjugation;Zymogen SUBCELLULAR LOCATION: Cytoplasm MISCELLANEOUS: The promiscuous caspase inhibitor p35 is neither cleaved by Dronc in vitro nor blocks Dronc activity in vivo. Q9XYM0,"PROTEIN NAMES: Adapter molecule Crk PROTEIN FAMILY: CRK family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the CRK family. FUNCTION: Adapter protein which interacts with C-terminal portion of mbc, homolog of human DOCK180. May play a role in cellular processes throughout development. KEYWORDS: Developmental protein;Reference proteome;Repeat;SH2 domain;SH3 domain " Q9XYS3,PROTEIN NAMES: Superoxide-generating NADPH oxidase heavy chain subunit A (NADPH oxidase A) (Superoxide-generating NADPH oxidase flavocytochrome A) ORGANISM: Dictyostelium discoideum (Social amoeba) FUNCTION: Critical component of the membrane-bound oxidase that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior (By similarity). KEYWORDS: Electron transport;FAD;Flavoprotein;Heme;Iron;Membrane;Metal-binding;NADP;Oxidoreductase;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Membrane ; Multi-pass membrane protein Q9XYS8,"PROTEIN NAMES: Lysosome membrane protein 2-A (Lysosome membrane protein II-1) (LIMP II-1) PROTEIN FAMILY: CD36 family ORGANISM: Dictyostelium discoideum (Social amoeba) SIMILARITY: Belongs to the CD36 family. FUNCTION: May act as a lysosomal receptor (By similarity). May be involved in macropinocytosis and fluid phase exocytosis. Binds to the anionic phospholipid phosphoinositol 4,5-bisphosphate, but not to phosphatidylcholine and only weakly to phosphatidylserine. KEYWORDS: Glycoprotein;Lysosome;Membrane;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Lysosome membrane ; Multi-pass membrane protein Note=Localizes to membranes of endolysosomal vesicles and macropinosomes. " Q9XYT4,"PROTEIN NAMES: Orchestin ORGANISM: Cryptorchestia cavimana (Amphipod) (Orchestia cavimana) FUNCTION: Plays a role in cuticle calcification. May induce precipitation of the calcium stored in the posterior caeca as calcium carbonate. KEYWORDS: Biomineralization;Calcium;Direct protein sequencing;Secreted;Signal SUBCELLULAR LOCATION: Secreted Note=During the premolt period, localizes to microvilli-rich apical regions and to the subnuclear region. During the postmolt period, localizes to the intercellular space, basal region of cells and calcium reabsorption spherules. MISCELLANEOUS: Binds calcium." Q9XYU1,"PROTEIN NAMES: DNA replication licensing factor Mcm3 (Minichromosome maintenance 3 protein) (DmMCM3) PROTEIN FAMILY: MCM family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the MCM family. FUNCTION: Acts as a component of the Mcm2-7 complex (Mcm complex) (Mcm complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built. The active ATPase sites in the Mcm2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. KEYWORDS: 3D-structure;ATP-binding;Cell cycle;Chromosome;DNA replication;DNA-binding;Helicase;Hydrolase;Nucleotide-binding;Nucleus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Nucleus Chromosome Note=Associated with chromatin before the formation of nuclei and detaches from it as DNA replication progresses. " Q9XZ08,"PROTEIN NAMES: Exostosin-3 (Protein brother of tout-velu) PROTEIN FAMILY: Glycosyltransferase 47 family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the glycosyltransferase 47 family. FUNCTION: Glycosyltransferase required for the biosynthesis of heparan-sulfate and responsible for the alternating addition of beta-1-4-linked glucuronic acid (GlcA) and alpha-1-4-linked N-acetylglucosamine (GlcNAc) units to nascent heparan sulfate chains. Plays a central role in diffusion of morphogens hedgehog (hh), wingless (wg) and Decapentaplegic (dpp) via its role in heparan sulfate proteoglycans (HSPGs) biosynthesis, HSPGs being required for movement of Hh, Dpp and wg morphogens. PATHWAY: Glycan metabolism; heparan sulfate biosynthesis. KEYWORDS: Developmental protein;Disulfide bond;Endoplasmic reticulum;Glycoprotein;Glycosyltransferase;Golgi apparatus;Manganese;Membrane;Metal-binding;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix;Wnt signaling pathway SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Single-pass type II membrane protein Golgi apparatus membrane ; Single-pass type II membrane protein Note=Localization to the Golgi may be regulated by sau. " Q9XZI6,"PROTEIN NAMES: Phosphatidylinositol-binding clathrin assembly protein unc-11 (AP180-like adaptor protein) (Uncoordinated protein 11) PROTEIN FAMILY: PICALM/SNAP91 family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the PICALM/SNAP91 family. FUNCTION: Assembly protein recruiting clathrin and adaptor protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. May be required to determine the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. Involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction. Required for the efficient targeting of the synaptic vesicle protein synaptobrevin. KEYWORDS: Alternative splicing;Coated pit;Cytoplasmic vesicle;Endocytosis;Golgi apparatus;Membrane;Reference proteome SUBCELLULAR LOCATION: Membrane, clathrin-coated pit Golgi apparatus Cytoplasmic vesicle, clathrin-coated vesicle Note=Colocalized with clathrin in the Golgi area. " Q9Y0H4,"PROTEIN NAMES: E3 ubiquitin-protein ligase Su(dx) (HECT-type E3 ubiquitin transferase Su(dx)) (Protein suppressor of deltex) ORGANISM: Drosophila melanogaster (Fruit fly) FUNCTION: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Down-regulates Notch/N signaling pathway, probably by promoting Notch ubiquitination, endocytosis and degradation. Involved in wing growth and leg joint formation. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: 3D-structure;Developmental protein;Notch signaling pathway;Reference proteome;Repeat;Transferase;Ubl conjugation pathway " Q9Y0I1,"PROTEIN NAMES: NuA4 complex subunit EAF3 homolog (Protein MRG15) ORGANISM: Drosophila melanogaster (Fruit fly) FUNCTION: Part of the Tip60 chromatin-remodeling complex which is involved in DNA repair. Upon induction of DNA double-strand breaks, this complex acetylates phosphorylated H2AV in nucleosomes and exchanges it with unmodified H2AV. KEYWORDS: 3D-structure;Chromatin regulator;DNA damage;DNA repair;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " Q9Y117,"PROTEIN NAMES: Polypeptide N-acetylgalactosaminyltransferase 3 (pp-GaNTase 3) (Protein-UDP acetylgalactosaminyltransferase 3) (UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 3) PROTEIN FAMILY: Glycosyltransferase 2 family, GalNAc-T subfamily ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily. FUNCTION: Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. It can both act as a peptide transferase that transfers GalNAc onto unmodified peptide substrates, and as a glycopeptide transferase that requires the prior addition of a GalNAc on a peptide before adding additional GalNAc moieties. Prefers EA2 as substrate. Has weak activity toward Muc5AC-3, -13 and -3/13 substrates. Plays a critical role in the regulation of integrin-mediated cell adhesion during wing development by influencing, via glycosylation, the secretion and localization of the integrin ligand Tig to the basal cell layer interface. Might have a role in protein O-glycosylation in the Golgi and thereby in establishing and/or maintaining a proper secretory apparatus structure. Together with Pgant35A, regulates integrin levels and activity-dependent integrin signaling at the synapse in neurons and muscles. PATHWAY: Protein modification; protein glycosylation. KEYWORDS: Disulfide bond;Glycoprotein;Glycosyltransferase;Golgi apparatus;Lectin;Manganese;Membrane;Metal-binding;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus membrane ; Single-pass type II membrane protein " Q9Y194,"PROTEIN NAMES: Sphingosine-1-phosphate lyase (S1PL) (SP-lyase) (SPL) (Sphingosine-1-phosphate aldolase) PROTEIN FAMILY: Group II decarboxylase family, Sphingosine-1-phosphate lyase subfamily ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the group II decarboxylase family. Sphingosine-1-phosphate lyase subfamily. FUNCTION: Cleaves phosphorylated sphingoid bases (PSBs or S1Ps) at the C2-3 carbon bond to yield a fatty aldehyde and phosphoethanolamine. These bioactive sphingolipid metabolites are essential for normal development, intestinal integrity, growth and reproduction. PATHWAY: Lipid metabolism; sphingolipid metabolism. KEYWORDS: Endoplasmic reticulum;Lipid metabolism;Lyase;Membrane;Pyridoxal phosphate;Reference proteome;Signal-anchor;Sphingolipid metabolism;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Single-pass type III membrane protein " Q9Y1I2,"PROTEIN NAMES: Vacuolar protein sorting-associated protein 33A (Protein carnation) PROTEIN FAMILY: STXBP/unc-18/SEC1 family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the STXBP/unc-18/SEC1 family. FUNCTION: Core component of the class C core vacuole/endosome tethering (CORVET) and the homotypic fusion and vacuole protein sorting (HOPS) tethering complexes involved in endo-lysosomal vesicle trafficking and lysosome biogenesis. The CORVET complex facilitates docking and fusion of endosomal vesicles during endosome maturation, acts upstream of HOPS, but is not involved in autophagic flux. The CORVET complex may cooperate with the early endosomal tether Rbsn-5 to mediate endosomal fusion. The HOPS complex facilitates docking and fusion of lysosomes with late endosomes and several other types of vesicles. The HOPS complex is also involved in autophagy and crinophagy (the elimination of unused secretory granules through their fusion with lysosomes). The HOPS complex probably instigates autophagosome-lysosome fusion by binding autophagosome associated Syx17/syntaxin 17 and promoting assembly of the trans-SNARE complex. Independent of Syx17/syntaxin 17 HOPS is involved in biosynthetic transport to lysosomes and lysosome-related organelles such as eye-pigment granules. Required for endocytic degradation of boss/bride of sevenless and N/Notch in developing ommatidia. KEYWORDS: Autophagy;Endosome;Lysosome;Membrane;Protein transport;Reference proteome;Transport SUBCELLULAR LOCATION: Early endosome Late endosome membrane ; Peripheral membrane protein ; Cytoplasmic side Lysosome membrane ; Peripheral membrane protein ; Cytoplasmic side Note=Cytoplasmic, peripheral membrane protein associated with late endosomes/lysosomes. MISCELLANEOUS: The name 'carnation' was given due to the eye color phenotype caused by hypomorphic mutations in the car gene." Q9Y1J3,"PROTEIN NAMES: 3-phosphoinositide-dependent protein kinase 1 (Pdk-class protein kinase 1) PROTEIN FAMILY: Protein kinase superfamily, AGC Ser/Thr protein kinase family, PDPK1 subfamily ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDPK1 subfamily. FUNCTION: Involved in the daf-2/insulin receptor-like transduction pathway, which controls longevity and prevents developmental arrest at the dauer stage. Phosphorylates and activates sgk-1, akt-1 and akt-2. KEYWORDS: Alternative splicing;ATP-binding;Coiled coil;Cytoplasm;Developmental protein;Kinase;Nucleotide-binding;Reference proteome;Serine/threonine-protein kinase;Transferase SUBCELLULAR LOCATION: Cytoplasm " Q9Y232,"PROTEIN NAMES: Chromodomain Y-like protein (CDY-like) (Crotonyl-CoA hydratase) ORGANISM: Homo sapiens (Human) FUNCTION: [Isoform 2]: Chromatin reader protein that recognizes and binds histone H3 trimethylated at 'Lys-9', dimethylated at 'Lys-27' and trimethylated at 'Lys-27' (H3K9me3, H3K27me2 and H3K27me3, respectively). Part of multimeric repressive chromatin complexes, where it is required for transmission and restoration of repressive histone marks, thereby preserving the epigenetic landscape. Required for chromatin targeting and maximal enzymatic activity of Polycomb repressive complex 2 (PRC2); acts as a positive regulator of PRC2 activity by bridging the pre-existing histone H3K27me3 and newly recruited PRC2 on neighboring nucleosomes. Acts as a corepressor for REST by facilitating histone-lysine N-methyltransferase EHMT2 recruitment and H3K9 dimethylation at REST target genes for repression. Involved in X chromosome inactivation in females: recruited to Xist RNA-coated X chromosome and facilitates propagation of H3K9me2 by anchoring EHMT2 (By similarity). Promotes EZH2 accumulation and H3K27me3 methylation at DNA double strand breaks (DSBs), thereby facilitating transcriptional repression at sites of DNA damage and homology-directed repair of DSBs. Required for neuronal migration during brain development by repressing expression of RHOA (By similarity). By repressing the expression of SCN8A, contributes to the inhibition of intrinsic neuronal excitability and epileptogenesis (By similarity). In addition to acting as a chromatin reader, acts as a hydro-lyase. Shows crotonyl-coA hydratase activity by mediating the conversion of crotonyl-CoA ((2E)-butenoyl-CoA) to beta-hydroxybutyryl-CoA (3-hydroxybutanoyl-CoA), thereby acting as a negative regulator of histone crotonylation. Histone crotonylation is required during spermatogenesis; down-regulation of histone crotonylation by CDYL regulates the reactivation of sex chromosome-linked genes in round spermatids and histone replacement in elongating spermatids (By similarity). By regulating histone crotonylation and trimethylation of H3K27, may be involved in stress-induced depression-like behaviors, possibly by regulating VGF expression (By similarity).; FUNCTION: [Isoform 1]: Not able to recognize and bind histone H3K9me3, histone H3K27me2 and histone H3K27me3, due to the presence of a N-terminal extension that inactivates the chromo domain.; FUNCTION: [Isoform 3]: Not able to recognize and bind histone H3K9me3, histone H3K27me2 and histone H3K27me3, due to the absence of the chromo domain. Acts as a negative regulator of isoform 2 by displacing isoform 2 from chromatin. KEYWORDS: 3D-structure;Alternative splicing;Chromosome;Differentiation;Lyase;Methylation;Nucleus;Phosphoprotein;Reference proteome;Repressor;Spermatogenesis;Transcription;Transcription regulation SUBCELLULAR LOCATION: [Isoform 2]: Nucleus Chromosome Note=Recognizes and binds histone H3 trimethylated at 'Lys-9', dimethylated at 'Lys-27' and trimethylated at 'Lys-27' (H3K9me3, H3K27me2 and H3K27me3, respectively) on chromatin. Multimerization is required for chromatin-binding. Recruited to sites of DNA double strand breaks in a PARP1-dependent fashion. MISCELLANEOUS: [Isoform 2]: Major isoform." Q9Y252,"PROTEIN NAMES: E3 ubiquitin-protein ligase RNF6 PROTEIN FAMILY: RNF12 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the RNF12 family. FUNCTION: E3 ubiquitin-protein ligase mediating 'Lys-48'-linked polyubiquitination of LIMK1 and its subsequent targeting to the proteasome for degradation (By similarity). Negatively regulates axonal outgrowth through regulation of the LIMK1 turnover (By similarity). Mediates 'Lys-6' and 'Lys-27'-linked polyubiquitination of AR/androgen receptor thereby modulating its transcriptional activity. May also bind DNA and function as a transcriptional regulator (By similarity). Mediates polyubiquitination of QKI in macrophages, leading to its degradation (By similarity). PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Alternative splicing;Cell projection;Cytoplasm;Metal-binding;Nucleus;Reference proteome;Transferase;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Cytoplasm Cell projection, axon Nucleus, PML body Note=Localizes to the PML nuclear bodies in Sertoli cells. " Q9Y253,"PROTEIN NAMES: DNA polymerase eta (RAD30 homolog A) (Xeroderma pigmentosum variant type protein) PROTEIN FAMILY: DNA polymerase type-Y family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the DNA polymerase type-Y family. FUNCTION: DNA polymerase specifically involved in the DNA repair by translesion synthesis (TLS). Due to low processivity on both damaged and normal DNA, cooperates with the heterotetrameric (REV3L, REV7, POLD2 and POLD3) POLZ complex for complete bypass of DNA lesions. Inserts one or 2 nucleotide(s) opposite the lesion, the primer is further extended by the tetrameric POLZ complex. In the case of 1,2-intrastrand d(GpG)-cisplatin cross-link, inserts dCTP opposite the 3' guanine. Particularly important for the repair of UV-induced pyrimidine dimers. Although inserts the correct base, may cause base transitions and transversions depending upon the context. May play a role in hypermutation at immunoglobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have any lyase activity, preventing the release of the 5'-deoxyribose phosphate (5'-dRP) residue. This covalent trapping of the enzyme by the 5'-dRP residue inhibits its DNA synthetic activity during base excision repair, thereby avoiding high incidence of mutagenesis. Targets POLI to replication foci. KEYWORDS: 3D-structure;Alternative splicing;Direct protein sequencing;Disease variant;DNA damage;DNA repair;DNA replication;DNA synthesis;DNA-binding;DNA-directed DNA polymerase;Isopeptide bond;Magnesium;Metal-binding;Mutator protein;Nucleotidyltransferase;Nucleus;Reference proteome;Schiff base;Transferase;Ubl conjugation;Xeroderma pigmentosum;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Note=Binding to ubiquitinated PCNA mediates colocalization to replication foci during DNA replication and persists at sites of stalled replication forks following UV irradiation. After UV irradiation, recruited to DNA damage sites within 1 hour, to a maximum of about 80%; this recruitment may not be not restricted to cells active in DNA replication. Colocalizes with TRAIP to nuclear foci. " Q9Y2C5,"PROTEIN NAMES: Probable small intestine urate exporter (Solute carrier family 17 member 4) PROTEIN FAMILY: Major facilitator superfamily, Sodium/anion cotransporter family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the major facilitator superfamily. Sodium/anion cotransporter family. FUNCTION: Acts as a membrane potential-dependent organic anion transporter, the transport requires a low concentration of chloride ions. Mediates chloride-dependent transport of urate. Mediates sodium-independent high affinity transport of thyroid hormones including L-thyroxine (T4) and 3,3',5-triiodo-L-thyronine (T3). Can actively transport inorganic phosphate into cells via Na(+) cotransport. KEYWORDS: Alternative splicing;Cell membrane;Glycoprotein;Ion transport;Membrane;Reference proteome;Sodium;Sodium transport;Symport;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Apical cell membrane ; Multi-pass membrane protein Note=Apical in the intestinal brush border. " Q9Y2D4,PROTEIN NAMES: Exocyst complex component 6B (Exocyst complex component Sec15B) (SEC15-like protein 2) PROTEIN FAMILY: SEC15 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the SEC15 family. FUNCTION: Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane. KEYWORDS: Alternative splicing;Coiled coil;Disease variant;Dwarfism;Exocytosis;Protein transport;Reference proteome;Transport Q9Y2I9,PROTEIN NAMES: TBC1 domain family member 30 ORGANISM: Homo sapiens (Human) FUNCTION: May act as a GTPase-activating protein for Rab family protein(s). KEYWORDS: Alternative splicing;Cell membrane;Membrane;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cell membrane ; Peripheral membrane protein Q9Y2M2,PROTEIN NAMES: Protein SSUH2 homolog (Protein ssu-2 homolog) ORGANISM: Homo sapiens (Human) FUNCTION: Plays a role in odontogenesis. KEYWORDS: Alternative splicing;Cytoplasm;Disease variant;Nucleus;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Nucleus Q9Y2R4,"PROTEIN NAMES: Probable ATP-dependent RNA helicase DDX52 (ATP-dependent RNA helicase ROK1-like) (DEAD box protein 52) PROTEIN FAMILY: DEAD box helicase family, DDX52/ROK1 subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the DEAD box helicase family. DDX52/ROK1 subfamily. FUNCTION: Required for efficient ribosome biogenesis (By similarity). May control cell cycle progression by regulating translation of mRNAs that contain a terminal oligo pyrimidine (TOP) motif in their 5' UTRs, such as GTPBP4 (By similarity). KEYWORDS: 3D-structure;Acetylation;ATP-binding;Helicase;Hydrolase;Nucleotide-binding;Nucleus;Phosphoprotein;Reference proteome;RNA-binding SUBCELLULAR LOCATION: Nucleus, nucleolus " Q9Y2W1,"PROTEIN NAMES: Thyroid hormone receptor-associated protein 3 (BCLAF1 and THRAP3 family member 2) (Thyroid hormone receptor-associated protein complex 150 kDa component) (Trap150) PROTEIN FAMILY: BCLAF1/THRAP3 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the BCLAF1/THRAP3 family. FUNCTION: Involved in pre-mRNA splicing. Remains associated with spliced mRNA after splicing which probably involves interactions with the exon junction complex (EJC). Can trigger mRNA decay which seems to be independent of nonsense-mediated decay involving premature stop codons (PTC) recognition. May be involved in nuclear mRNA decay. Involved in regulation of signal-induced alternative splicing. During splicing of PTPRC/CD45 is proposed to sequester phosphorylated SFPQ from PTPRC/CD45 pre-mRNA in resting T-cells. Involved in cyclin-D1/CCND1 mRNA stability probably by acting as component of the SNARP complex which associates with both the 3'end of the CCND1 gene and its mRNA. Involved in response to DNA damage. Is excluced from DNA damage sites in a manner that parallels transcription inhibition; the function may involve the SNARP complex. Initially thought to play a role in transcriptional coactivation through its association with the TRAP complex; however, it is not regarded as a stable Mediator complex subunit. Cooperatively with HELZ2, enhances the transcriptional activation mediated by PPARG, maybe through the stabilization of the PPARG binding to DNA in presence of ligand. May play a role in the terminal stage of adipocyte differentiation. Plays a role in the positive regulation of the circadian clock. Acts as a coactivator of the CLOCK-BMAL1 heterodimer and promotes its transcriptional activator activity and binding to circadian target genes. KEYWORDS: Acetylation;Activator;ADP-ribosylation;ATP-binding;Biological rhythms;Direct protein sequencing;Isopeptide bond;Methylation;mRNA processing;mRNA splicing;Nucleotide-binding;Nucleus;Phosphoprotein;Receptor;Reference proteome;Transcription;Transcription regulation;Ubl conjugation SUBCELLULAR LOCATION: Nucleus Nucleus, nucleoplasm Nucleus speckle " Q9Y2W6,"PROTEIN NAMES: Tudor and KH domain-containing protein (Tudor domain-containing protein 2) PROTEIN FAMILY: Tdrkh family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the Tdrkh family. FUNCTION: Participates in the primary piRNA biogenesis pathway and is required during spermatogenesis to repress transposable elements and prevent their mobilization, which is essential for the germline integrity. The piRNA metabolic process mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Required for the final steps of primary piRNA biogenesis by participating in the processing of 31-37 nt intermediates into mature piRNAs. May act in pi-bodies and piP-bodies by transferring piRNA precursors or intermediates to or between these granules. KEYWORDS: 3D-structure;Alternative splicing;Cytoplasm;Differentiation;Isopeptide bond;Mitochondrion;Phosphoprotein;Reference proteome;Repeat;RNA-binding;RNA-mediated gene silencing;Spermatogenesis;Ubl conjugation SUBCELLULAR LOCATION: Cytoplasm Mitochondrion Note=Probable component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon activity during meiosis. Colocalizes with pi- and piP-bodies, a subset of the nuage which contains secondary piRNAs. Associated with mitochondria in the germline. " Q9Y2X9,"PROTEIN NAMES: Zinc finger protein 281 (GC-box-binding zinc finger protein 1) (Transcription factor ZBP-99) (Zinc finger DNA-binding protein 99) PROTEIN FAMILY: Krueppel C2H2-type zinc-finger protein family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein family. FUNCTION: Transcription repressor that plays a role in regulation of embryonic stem cells (ESCs) differentiation. Required for ESCs differentiation and acts by mediating autorepression of NANOG in ESCs: binds to the NANOG promoter and promotes association of NANOG protein to its own promoter and recruits the NuRD complex, which deacetylates histones. Not required for establishement and maintenance of ESCs (By similarity). Represses the transcription of a number of genes including GAST, ODC1 and VIM. Binds to the G-rich box in the enhancer region of these genes. KEYWORDS: Alternative splicing;Differentiation;DNA-binding;Isopeptide bond;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Repeat;Repressor;Transcription;Transcription regulation;Ubl conjugation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus " Q9Y2Y8,"PROTEIN NAMES: Proteoglycan 3 (Eosinophil major basic protein homolog) (Prepro-major basic protein homolog) (Prepro-MBPH) ORGANISM: Homo sapiens (Human) FUNCTION: Possesses similar cytotoxic and cytostimulatory activities to PRG2/MBP. In vitro, stimulates neutrophil superoxide production and IL8 release, and histamine and leukotriene C4 release from basophils. KEYWORDS: Disulfide bond;Lectin;Reference proteome;Signal SUBCELLULAR LOCATION: Cytoplasmic granule Note=Localized to the eosinophil secondary granule. " Q9Y2Z0,"PROTEIN NAMES: Protein SGT1 homolog (Protein 40-6-3) (Sgt1) (Suppressor of G2 allele of SKP1 homolog) PROTEIN FAMILY: SGT1 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the SGT1 family. FUNCTION: May play a role in ubiquitination and subsequent proteasomal degradation of target proteins. KEYWORDS: 3D-structure;Acetylation;Alternative splicing;Cytoplasm;Direct protein sequencing;Isopeptide bond;Nucleus;Phosphoprotein;Reference proteome;Repeat;TPR repeat;Ubl conjugation;Ubl conjugation pathway SUBCELLULAR LOCATION: Cytoplasm Nucleus Note=Translocates to the nucleus upon heat shock, requiring S100A6. " Q9Y2Z2,"PROTEIN NAMES: Protein MTO1 homolog, mitochondrial PROTEIN FAMILY: MnmG family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the MnmG family. FUNCTION: Involved in the 5-carboxymethylaminomethyl modification (mnm(5)s(2)U34) of the wobble uridine base in mitochondrial tRNAs. KEYWORDS: Alternative splicing;Cardiomyopathy;Disease variant;FAD;Flavoprotein;Methylation;Mitochondrion;Primary mitochondrial disease;Reference proteome;Transit peptide;tRNA processing SUBCELLULAR LOCATION: Mitochondrion " Q9Y2Z4,"PROTEIN NAMES: Tyrosine--tRNA ligase, mitochondrial (Tyrosyl-tRNA synthetase) (TyrRS) PROTEIN FAMILY: Class-I aminoacyl-tRNA synthetase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family. FUNCTION: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr). KEYWORDS: 3D-structure;Acetylation;Aminoacyl-tRNA synthetase;ATP-binding;Disease variant;Ligase;Mitochondrion;Nucleotide-binding;Primary mitochondrial disease;Protein biosynthesis;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Mitochondrion matrix " Q9Y3D2,"PROTEIN NAMES: Methionine-R-sulfoxide reductase B2, mitochondrial (MsrB2) PROTEIN FAMILY: MsrB Met sulfoxide reductase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the MsrB Met sulfoxide reductase family. FUNCTION: Methionine-sulfoxide reductase that specifically reduces methionine (R)-sulfoxide back to methionine. While in many cases, methionine oxidation is the result of random oxidation following oxidative stress, methionine oxidation is also a post-translational modification that takes place on specific residue. Upon oxidative stress, may play a role in the preservation of mitochondrial integrity by decreasing the intracellular reactive oxygen species build-up through its scavenging role, hence contributing to cell survival and protein maintenance. KEYWORDS: Metal-binding;Mitochondrion;Oxidoreductase;Reference proteome;Transit peptide;Zinc SUBCELLULAR LOCATION: Mitochondrion " Q9Y3R4,"PROTEIN NAMES: Sialidase-2 (Cytosolic sialidase) (N-acetyl-alpha-neuraminidase 2) PROTEIN FAMILY: Glycosyl hydrolase 33 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the glycosyl hydrolase 33 family. FUNCTION: Exo-alpha-sialidase that catalyzes the hydrolytic cleavage of the terminal sialic acid (N-acetylneuraminic acid, Neu5Ac) of a glycan moiety in the catabolism of glycolipids, glycoproteins and oligosacharides. Recognizes sialyl linkage positions of the glycan moiety as well as the supramolecular organization of the sialoglycoconjugate. Displays preference for alpha-(2->3)-sialylated GD1a and GT1B gangliosides over alpha-(2->8)-sialylated GD1b, in both monomeric forms and micelles. Hydrolyzes monomeric GM1 ganglioside, but has no activity toward the miscellar form. Has lower sialidase activity for glycoproteins such as fetuin and TF/transferrin that carry a mixture of alpha-(2->3) and alpha-(2->6)-sialyl linkages. Cleaves milk oligosaccharide alpha-(2->3)-sialyllactose, but is inactive toward alpha-(2->6)-sialyllactose isomer. Has no activity toward colominic acid, a homomer of alpha-(2->8)-linked Neu5Ac residues. KEYWORDS: 3D-structure;Carbohydrate metabolism;Cytoplasm;Glycosidase;Hydrolase;Lipid degradation;Lipid metabolism;Reference proteome;Repeat SUBCELLULAR LOCATION: Cytoplasm, cytosol " Q9Y4A8,"PROTEIN NAMES: Nuclear factor erythroid 2-related factor 3 (NF-E2-related factor 3) (NFE2-related factor 3) (Nuclear factor, erythroid derived 2, like 3) PROTEIN FAMILY: BZIP family, CNC subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the bZIP family. CNC subfamily. FUNCTION: Activates erythroid-specific, globin gene expression. KEYWORDS: Activator;DNA-binding;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " Q9Y4P8,"PROTEIN NAMES: WD repeat domain phosphoinositide-interacting protein 2 (WIPI-2) (WIPI49-like protein 2) PROTEIN FAMILY: WD repeat PROPPIN family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the WD repeat PROPPIN family. FUNCTION: Component of the autophagy machinery that controls the major intracellular degradation process by which cytoplasmic materials are packaged into autophagosomes and delivered to lysosomes for degradation. Involved in an early step of the formation of preautophagosomal structures. Binds and is activated by phosphatidylinositol 3-phosphate (PtdIns3P) forming on membranes of the endoplasmic reticulum upon activation of the upstream ULK1 and PI3 kinases. Mediates ER-isolation membranes contacts by interacting with the ULK1:RB1CC1 complex and PtdIns3P. Once activated, WIPI2 recruits at phagophore assembly sites the ATG12-ATG5-ATG16L1 complex that directly controls the elongation of the nascent autophagosomal membrane.; FUNCTION: [Isoform 4]: Recruits the ATG12-ATG5-ATG16L1 complex to omegasomes and preautophagosomal structures, resulting in ATG8 family proteins lipidation and starvation-induced autophagy. Isoform 4 is also required for autophagic clearance of pathogenic bacteria. Isoform 4 binds the membrane surrounding Salmonella and recruits the ATG12-5-16L1 complex, initiating LC3 conjugation, autophagosomal membrane formation, and engulfment of Salmonella. KEYWORDS: 3D-structure;Alternative splicing;Autophagy;Disease variant;Intellectual disability;Lipid-binding;Membrane;Phosphoprotein;Reference proteome;Repeat;WD repeat SUBCELLULAR LOCATION: Preautophagosomal structure membrane ; Peripheral membrane protein ; Cytoplasmic side Note=Localizes to omegasomes membranes which are endoplasmic reticulum connected structures at the origin of preautophagosomal structures. Enriched at preautophagosomal structure membranes in response to PtdIns3P. " Q9Y572,"PROTEIN NAMES: Receptor-interacting serine/threonine-protein kinase 3 (RIP-like protein kinase 3) (Receptor-interacting protein 3) (RIP-3) PROTEIN FAMILY: Protein kinase superfamily, TKL Ser/Thr protein kinase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. FUNCTION: Serine/threonine-protein kinase that activates necroptosis and apoptosis, two parallel forms of cell death. Necroptosis, a programmed cell death process in response to death-inducing TNF-alpha family members, is triggered by RIPK3 following activation by ZBP1. Activated RIPK3 forms a necrosis-inducing complex and mediates phosphorylation of MLKL, promoting MLKL localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. In addition to TNF-induced necroptosis, necroptosis can also take place in the nucleus in response to orthomyxoviruses infection: following ZBP1 activation, which senses double-stranded Z-RNA structures, nuclear RIPK3 catalyzes phosphorylation and activation of MLKL, promoting disruption of the nuclear envelope and leakage of cellular DNA into the cytosol (By similarity). Also regulates apoptosis: apoptosis depends on RIPK1, FADD and CASP8, and is independent of MLKL and RIPK3 kinase activity (By similarity). Phosphorylates RIPK1: RIPK1 and RIPK3 undergo reciprocal auto- and trans-phosphorylation. In some cell types, also able to restrict viral replication by promoting cell death-independent responses (By similarity). In response to Zika virus infection in neurons, promotes a cell death-independent pathway that restricts viral replication: together with ZBP1, promotes a death-independent transcriptional program that modifies the cellular metabolism via up-regulation expression of the enzyme ACOD1/IRG1 and production of the metabolite itaconate (By similarity). Itaconate inhibits the activity of succinate dehydrogenase, generating a metabolic state in neurons that suppresses replication of viral genomes (By similarity). RIPK3 binds to and enhances the activity of three metabolic enzymes: GLUL, GLUD1, and PYGL. These metabolic enzymes may eventually stimulate the tricarboxylic acid cycle and oxidative phosphorylation, which could result in enhanced ROS production.; FUNCTION: (Microbial infection) In case of herpes simplex virus 1/HHV-1 infection, forms heteromeric amyloid structures with HHV-1 protein RIR1/ICP6 which may inhibit RIPK3-mediated necroptosis, thereby preventing host cell death pathway and allowing viral evasion. KEYWORDS: 3D-structure;Alternative splicing;Apoptosis;ATP-binding;Cytoplasm;Host-virus interaction;Isopeptide bond;Kinase;Necrosis;Nucleotide-binding;Nucleus;Phosphoprotein;Reference proteome;Serine/threonine-protein kinase;Transferase;Ubl conjugation SUBCELLULAR LOCATION: Cytoplasm, cytosol Nucleus Note=Mainly cytoplasmic. Present in the nucleus in response to influenza A virus (IAV) infection. " Q9Y5I7,"PROTEIN NAMES: Claudin-16 (Paracellin-1) (PCLN-1) PROTEIN FAMILY: Claudin family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the claudin family. FUNCTION: Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity. Involved in paracellular magnesium reabsorption. Required for a selective paracellular conductance. May form, alone or in partnership with other constituents, an intercellular pore permitting paracellular passage of magnesium and calcium ions down their electrochemical gradients. Alternatively, it could be a sensor of magnesium concentration that could alter paracellular permeability mediated by other factors. KEYWORDS: Cell junction;Cell membrane;Disease variant;Ion transport;Magnesium;Membrane;Primary hypomagnesemia;Reference proteome;Tight junction;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell junction, tight junction. Cell membrane; Multi-pass membrane protein. " Q9Y5J1,"PROTEIN NAMES: U3 small nucleolar RNA-associated protein 18 homolog (WD repeat-containing protein 50) PROTEIN FAMILY: WD repeat UTP18 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the WD repeat UTP18 family. FUNCTION: Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome. Involved in nucleolar processing of pre-18S ribosomal RNA. KEYWORDS: 3D-structure;Isopeptide bond;Nucleus;Phosphoprotein;Reference proteome;Repeat;rRNA processing;Ubl conjugation;WD repeat SUBCELLULAR LOCATION: Nucleus, nucleolus " Q9Y5X2,PROTEIN NAMES: Sorting nexin-8 PROTEIN FAMILY: Sorting nexin family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the sorting nexin family. FUNCTION: May be involved in several stages of intracellular trafficking. May play a role in intracellular protein transport from early endosomes to the trans-Golgi network. KEYWORDS: Endosome;Lipid-binding;Membrane;Phosphoprotein;Protein transport;Reference proteome;Transport SUBCELLULAR LOCATION: Early endosome membrane ; Peripheral membrane protein ; Cytoplasmic side Note=Colocalizes with retromer components. Q9Y5X5,"PROTEIN NAMES: Neuropeptide FF receptor 2 (G-protein coupled receptor 74) (G-protein coupled receptor HLWAR77) (Neuropeptide G-protein coupled receptor) PROTEIN FAMILY: G-protein coupled receptor 1 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the G-protein coupled receptor 1 family. FUNCTION: Receptor for NPAF (A-18-F-amide) and NPFF (F-8-F-amide) neuropeptides, also known as morphine-modulating peptides. Can also be activated by a variety of naturally occurring or synthetic FMRF-amide like ligands. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. KEYWORDS: Alternative splicing;Cell membrane;Disulfide bond;G-protein coupled receptor;Glycoprotein;Membrane;Receptor;Reference proteome;Transducer;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. " Q9Y603,PROTEIN NAMES: Transcription factor ETV7 (ETS translocation variant 7) (ETS-related protein Tel2) (Tel-related Ets factor) (Transcription factor Tel-2) PROTEIN FAMILY: ETS family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the ETS family. FUNCTION: Transcriptional repressor; binds to the DNA sequence 5'-CCGGAAGT-3'. Isoform A does not seem to have a repressor activity. Isoform C does not seem to have a repressor activity. KEYWORDS: Alternative splicing;DNA-binding;Nucleus;Reference proteome;Repressor;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus. Q9Y606,PROTEIN NAMES: Pseudouridylate synthase 1 homolog (tRNA pseudouridine synthase 1) (tRNA pseudouridine(38-40) synthase) (tRNA pseudouridylate synthase I) (tRNA-uridine isomerase I) PROTEIN FAMILY: TRNA pseudouridine synthase TruA family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the tRNA pseudouridine synthase TruA family. FUNCTION: Pseudouridylate synthase that catalyzes pseudouridylation of tRNAs and mRNAs. Acts on positions 27/28 in the anticodon stem and also positions 34 and 36 in the anticodon of an intron containing tRNA. Also catalyzes pseudouridylation of mRNAs: mediates pseudouridylation of mRNAs with the consensus sequence 5'-UGUAG-3'. Acts as a regulator of pre-mRNA splicing by mediating pseudouridylation of pre-mRNAs at locations associated with alternatively spliced regions. Pseudouridylation of pre-mRNAs near splice sites directly regulates mRNA splicing and mRNA 3'-end processing. Involved in regulation of nuclear receptor activity through pseudouridylation of SRA1 mRNA. KEYWORDS: 3D-structure;Alternative splicing;Cytoplasm;Disease variant;Isomerase;Mitochondrion;mRNA processing;mRNA splicing;Nucleus;Phosphoprotein;Primary mitochondrial disease;Reference proteome;Transit peptide;tRNA processing SUBCELLULAR LOCATION: [Isoform 1]: Mitochondrion.; SUBCELLULAR LOCATION: [Isoform 2]: Nucleus Cytoplasm Q9Y692,PROTEIN NAMES: Glucocorticoid modulatory element-binding protein 1 (GMEB-1) (DNA-binding protein p96PIF) (Parvovirus initiation factor p96) (PIF p96) ORGANISM: Homo sapiens (Human) FUNCTION: Trans-acting factor that binds to glucocorticoid modulatory elements (GME) present in the TAT (tyrosine aminotransferase) promoter and increases sensitivity to low concentrations of glucocorticoids. Binds also to the transferrin receptor promoter. Essential auxiliary factor for the replication of parvoviruses. KEYWORDS: 3D-structure;Acetylation;Alternative splicing;Coiled coil;Cytoplasm;Direct protein sequencing;DNA-binding;Metal-binding;Nucleus;Reference proteome;Transcription;Transcription regulation;Zinc SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=May be also cytoplasmic. Q9Y6D9,"PROTEIN NAMES: Mitotic spindle assembly checkpoint protein MAD1 (Mitotic arrest deficient 1-like protein 1) (MAD1-like protein 1) (Mitotic checkpoint MAD1 protein homolog) (HsMAD1) (hMAD1) (Tax-binding protein 181) PROTEIN FAMILY: MAD1 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the MAD1 family. FUNCTION: Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. Forms a heterotetrameric complex with the closed conformation form of MAD2L1 (C-MAD2) at unattached kinetochores during prometaphase, recruits an open conformation of MAD2L1 (O-MAD2) and promotes the conversion of O-MAD2 to C-MAD2, which ensures mitotic checkpoint signaling.; FUNCTION: [Isoform 3]: Sequesters MAD2L1 in the cytoplasm preventing its function as an activator of the mitotic spindle assembly checkpoint (SAC) resulting in SAC impairment and chromosomal instability in hepatocellular carcinomas. KEYWORDS: 3D-structure;Acetylation;Alternative splicing;Cell cycle;Cell division;Centromere;Chromosome;Coiled coil;Cytoplasm;Cytoskeleton;Disease variant;Isopeptide bond;Kinetochore;Mitosis;Nucleus;Phosphoprotein;Reference proteome;Ubl conjugation SUBCELLULAR LOCATION: Nucleus Chromosome, centromere, kinetochore Nucleus envelope Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Cytoplasm, cytoskeleton, spindle Cytoplasm, cytoskeleton, spindle pole Note=Co-localizes with TPR at the nucleus envelope during interphase and throughout the cell cycle. From the beginning to the end of mitosis, it is seen to move from a diffusely nuclear distribution to the centrosome, to the spindle midzone and finally to the midbody. Localizes to kinetochores during prometaphase. Does not localize to kinetochores during metaphase. Colocalizes with NEK2 at the kinetochore. Colocalizes with IK at spindle poles during metaphase and anaphase.; SUBCELLULAR LOCATION: [Isoform 3]: Cytoplasm " Q9Y6F9,"PROTEIN NAMES: Protein Wnt-6 PROTEIN FAMILY: Wnt family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the Wnt family. FUNCTION: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. Together with CAV1 may promote chemoresistance of gastric cancer cells to DNA-damaging anthracycline drugs through the activation of the canonical Wnt receptor signaling pathway. KEYWORDS: Developmental protein;Disulfide bond;Extracellular matrix;Glycoprotein;Lipoprotein;Reference proteome;Secreted;Signal;Wnt signaling pathway SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix. " Q9Y6I9,"PROTEIN NAMES: Testis-expressed protein 264 (Putative secreted protein Zsig11) ORGANISM: Homo sapiens (Human) FUNCTION: Major reticulophagy (also called ER-phagy) receptor that acts independently of other candidate reticulophagy receptors to remodel subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover. The ATG8-containing isolation membrane (IM) cradles a tubular segment of TEX264-positive ER near a three-way junction, allowing the formation of a synapse of 2 juxtaposed membranes with trans interaction between the TEX264 and ATG8 proteins. Expansion of the IM would extend the capture of ER, possibly through a 'zipper-like' process involving continued trans TEX264-ATG8 interactions, until poorly understood mechanisms lead to the fission of relevant membranes and, ultimately, autophagosomal membrane closure. Also involved in the repair of covalent DNA-protein cross-links (DPCs) during DNA synthesis: acts by bridging VCP/p97 to covalent DNA-protein cross-links (DPCs) and initiating resolution of DPCs by SPRTN. KEYWORDS: 3D-structure;Autophagy;Chromosome;Cytoplasm;Cytoplasmic vesicle;DNA damage;DNA repair;Endoplasmic reticulum;Membrane;Nucleus;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Single-pass type III membrane protein Cytoplasmic vesicle, autophagosome Cytoplasm, cytosol Nucleus Chromosome Note=Is trafficked from tubular ER to growing autophagosomes via its cytosolic LIR motif. Also found in the cytosol, nucleus and chromatin. In response to formation of covalent DNA-protein cross-links (DPCs), localizes to the nuclear periphery, and associates with DNA replication forks. " Q9Y6J9,"PROTEIN NAMES: TAF6-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 6L (TAF6L) (PCAF-associated factor 65-alpha) (PAF65-alpha) PROTEIN FAMILY: TAF6 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the TAF6 family. FUNCTION: Functions as a component of the PCAF complex. The PCAF complex is capable of efficiently acetylating histones in a nucleosomal context. The PCAF complex could be considered as the human version of the yeast SAGA complex (Probable). With TAF5L, acts as an epigenetic regulator essential for somatic reprogramming. Regulates target genes through H3K9ac deposition and MYC recruitment which trigger MYC regulatory network to orchestrate gene expression programs to control embryonic stem cell state. Functions with MYC to activate target gene expression through RNA polymerase II pause release (By similarity). KEYWORDS: 3D-structure;Direct protein sequencing;Methylation;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " Q9Y738,"PROTEIN NAMES: Centromere protein mis12 (NMS complex subunit mis12) PROTEIN FAMILY: Mis12 family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the mis12 family. FUNCTION: Acts as a component of the NMS (Ndc80-MIND-Spc7) super complex which has a role in kinetochore function during late meiotic prophase and throughout the mitotic cell cycle. Required for correct segregation of chromosomes and for maintaining the inner centromere structure. KEYWORDS: 3D-structure;Cell cycle;Cell division;Centromere;Chromosome;Coiled coil;Kinetochore;Meiosis;Mitosis;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Chromosome, centromere Chromosome, centromere, kinetochore " Q9Y7J6,"PROTEIN NAMES: Serine/threonine-protein kinase ppk21 PROTEIN FAMILY: Protein kinase superfamily, AGC Ser/Thr protein kinase family, PDPK1 subfamily ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDPK1 subfamily. KEYWORDS: ATP-binding;Cytoplasm;Cytoskeleton;Kinase;Nucleotide-binding;Nucleus;Phosphoprotein;Reference proteome;Serine/threonine-protein kinase;Transferase SUBCELLULAR LOCATION: Cytoplasm Nucleus Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body Note=Located at the cell tips and septum. " Q9Y7Q6,"PROTEIN NAMES: Histone-lysine N-methyltransferase, H3 lysine-37 specific PROTEIN FAMILY: Class V-like SAM-binding methyltransferase superfamily ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the class V-like SAM-binding methyltransferase superfamily. FUNCTION: Histone lysine methyltransferase that specifically mono-, di-, and trimethylates 'Lys-37' of histone H3 to regulate sporulation. KEYWORDS: 3D-structure;Cytoplasm;Methyltransferase;Nucleus;Reference proteome;S-adenosyl-L-methionine;Sporulation;Transferase SUBCELLULAR LOCATION: Cytoplasm Nucleus " Q9Y7R4,"PROTEIN NAMES: Histone-lysine N-methyltransferase, H3 lysine-4 specific (COMPASS component set1) (Lysine N-methyltransferase 2) (SET domain-containing protein 1) (Set1 complex component set1) (Set1C component set1) (Spset1) PROTEIN FAMILY: Class V-like SAM-binding methyltransferase superfamily ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the class V-like SAM-binding methyltransferase superfamily. FUNCTION: Catalytic component of the COMPASS (Set1C) complex that specifically mono-, di- and trimethylates histone H3 to form H3K4me1/2/3. Binds RNA which might negatively affect its histone methyltransferase activity (By similarity). COMPASS recognizes ubiquitinated H2B on one face of the nucleosome which stimulates the methylation of H3 on the opposing face (By similarity). Methylation promotes maintenance of active chromatin states at euchromatic chromosomal domains and is present throughout the cell cycle. Plays a role in telomere maintenance and DNA repair in an ATM kinase rad3-dependent pathway. Required for efficient telomeric and centromeric silencing. KEYWORDS: Chromatin regulator;Chromosome;Methyltransferase;Nucleus;Reference proteome;RNA-binding;S-adenosyl-L-methionine;Transferase SUBCELLULAR LOCATION: Nucleus Chromosome " Q9Y7S9,"PROTEIN NAMES: Alpha-amylase 3 (1,4-alpha-D-glucan glucanohydrolase) PROTEIN FAMILY: Glycosyl hydrolase 13 family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the glycosyl hydrolase 13 family. FUNCTION: Has a role in cell wall biosynthesis where it is involved in maintaining cell wall strength and shape. KEYWORDS: Calcium;Carbohydrate metabolism;Cell membrane;Disulfide bond;Glycoprotein;Glycosidase;GPI-anchor;Hydrolase;Lipoprotein;Membrane;Metal-binding;Reference proteome;Signal SUBCELLULAR LOCATION: Cell membrane ; Lipid-anchor, GPI-anchor " Q9Y7Z5,PROTEIN NAMES: Tetra-spanning protein 1 PROTEIN FAMILY: PER33/POM33 family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the PER33/POM33 family. FUNCTION: Required for the correct positioning of the cellular division plane by delimiting the actomyosin ring assembly at the cell equator. KEYWORDS: Cell cycle;Cell division;Endoplasmic reticulum;Glycoprotein;Golgi apparatus;Membrane;Nucleus;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Nucleus membrane; Multi-pass membrane protein. Note=Enriched at the cell equator during mitosis. Q9Y871,PROTEIN NAMES: Feruloyl esterase B (Cinnamoyl ester hydrolase) (Esterase A) (EstA) (Ferulic acid esterase B) ORGANISM: Piromyces equi FUNCTION: Involved in degradation of plant cell walls. Hydrolyzes of the feruloyl-arabinose ester bond in arabinoxylans as well as the feruloyl-galactose and feruloyl-arabinose ester bonds in pectin. KEYWORDS: Carbohydrate metabolism;Glycoprotein;Hydrolase;Polysaccharide degradation;Repeat;Secreted;Serine esterase;Signal;Xylan degradation SUBCELLULAR LOCATION: Secreted. Q9Y899,"PROTEIN NAMES: Calcium/calmodulin-dependent protein kinase cmkB (CaMK B) (CaMKI/IV homolog) PROTEIN FAMILY: Protein kinase superfamily, CAMK Ser/Thr protein kinase family, CaMK subfamily ORGANISM: Emericella nidulans (Aspergillus nidulans) SIMILARITY: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily. FUNCTION: Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK1 signaling cascade. Required in G1-phase of the cell cycle for proper timing of the initial nuclear division after germination, but not for subsequent mitoses. Required for the normal temporal regulation of nimX activity. KEYWORDS: Allosteric enzyme;ATP-binding;Calmodulin-binding;Cell cycle;Cell division;Kinase;Nucleotide-binding;Phosphoprotein;Serine/threonine-protein kinase;Transferase " Q9Y8G3,PROTEIN NAMES: mRNA export factor mex67 PROTEIN FAMILY: NXF family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the NXF family. FUNCTION: Involved in the export of mRNA from the nucleus to the cytoplasm. KEYWORDS: Cytoplasm;Leucine-rich repeat;mRNA transport;Nucleus;Phosphoprotein;Reference proteome;Repeat;Transport SUBCELLULAR LOCATION: Nucleus Cytoplasm Note=Localizes at both the nuclear and cytoplasmic site of the pores. Shuttles between the nucleus and the cytoplasm. Q9Y948,"PROTEIN NAMES: Deoxyribose-phosphate aldolase (DERA) (2-deoxy-D-ribose 5-phosphate aldolase) (Phosphodeoxyriboaldolase) (Deoxyriboaldolase) PROTEIN FAMILY: DeoC/FbaB aldolase family, DeoC type 1 subfamily ORGANISM: Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1) SIMILARITY: Belongs to the DeoC/FbaB aldolase family. DeoC type 1 subfamily. FUNCTION: Catalyzes a reversible aldol reaction between acetaldehyde and D-glyceraldehyde 3-phosphate to generate 2-deoxy-D-ribose 5-phosphate. PATHWAY: Carbohydrate degradation; 2-deoxy-D-ribose 1-phosphate degradation; D-glyceraldehyde 3-phosphate and acetaldehyde from 2-deoxy-alpha-D-ribose 1-phosphate: step 2/2. KEYWORDS: 3D-structure;Cytoplasm;Direct protein sequencing;Lyase;Reference proteome;Schiff base SUBCELLULAR LOCATION: Cytoplasm MISCELLANEOUS: The enzyme is also highly resistant to organic solvents such as ethanol, methanol, N,N-dimethylformamide, and Me(2)SO at 50 degrees Celsius. Loss of activity is not observed in the presence of these reagents even at a concentration as high as 40%." Q9YBL2,"PROTEIN NAMES: Protein CysO (Cystathionine beta-synthase) (Cysteine synthase) (O-acetylserine sulfhydrylase) (O-phosphoserine sulfhydrylase) (Serine sulfhydrase) PROTEIN FAMILY: Cysteine synthase/cystathionine beta-synthase family ORGANISM: Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1) SIMILARITY: Belongs to the cysteine synthase/cystathionine beta-synthase family. FUNCTION: Cysteine synthase that can also catalyze the synthesis of S-sulfo-L-cysteine from thiosulfate and O(3)-acetyl-L-serine, as well as the sulfhydrylation of L-serine by sulfide. PATHWAY: Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-serine: step 2/2. KEYWORDS: 3D-structure;Amino-acid biosynthesis;Cysteine biosynthesis;Direct protein sequencing;Lyase;Pyridoxal phosphate;Reference proteome;Transferase " Q9YGW7,"PROTEIN NAMES: Deleted in azoospermia-like (DAZ-like protein) (zDazl) PROTEIN FAMILY: RRM DAZ family ORGANISM: Danio rerio (Zebrafish) (Brachydanio rerio) SIMILARITY: Belongs to the RRM DAZ family. FUNCTION: RNA-binding protein involved in gametogenesis in both males and females. Acts by binding to the 3'-UTR of mRNA, specifically recognizing GUU triplets, and promoting the translation of key transcripts. Establishes oocyte polarity through interaction with Bucky ball (BUC) (Probable). Interacts with Bucky ball (BUC) mRNA to mediate Balbiani body formation and oocyte polarity during early oogenesis (By similarity). KEYWORDS: Activator;Cytoplasm;Developmental protein;Differentiation;Oogenesis;Reference proteome;RNA-binding;Spermatogenesis;Translation regulation SUBCELLULAR LOCATION: Cytoplasm " Q9Z0E3,"PROTEIN NAMES: Autoimmune regulator (Autoimmune polyendocrinopathy candidiasis ectodermal dystrophy protein homolog) (APECED protein homolog) ORGANISM: Mus musculus (Mouse) FUNCTION: Transcription factor playing an essential role to promote self-tolerance in the thymus by regulating the expression of a wide array of self-antigens that have the commonality of being tissue-restricted in their expression pattern in the periphery, called tissue restricted antigens (TRA) (Probable). Binds to G-doublets in an A/T-rich environment; the preferred motif is a tandem repeat of 5'-. ATTGGTTA-3' combined with a 5'-TTATTA-3' box. Binds to nucleosomes (By similarity). Binds to chromatin and interacts selectively with histone H3 that is not methylated at 'Lys-4', not phosphorylated at 'Thr-3' and not methylated at 'Arg-2'. Functions as a sensor of histone H3 modifications that are important for the epigenetic regulation of gene expression. Mainly expressed by medullary thymic epithelial cells (mTECs), induces the expression of thousands of tissue-restricted proteins, which are presented on major histocompatibility complex class I (MHC-I) and MHC-II molecules to developing T-cells percolating through the thymic medulla (By similarity). Also induces self-tolerance through other mechanisms such as the regulation of the mTEC differentiation program. Controls the medullary accumulation of thymic dendritic cells and the development of regulatory T-cell through the regulation of XCL1 expression. Regulates the production of CCR4 and CCR7 ligands in medullary thymic epithelial cells and alters the coordinated maturation and migration of thymocytes. In thimic B-cells, allows the presentation of licensing-dependent endogenous self-anitgen for negative selection. In secondary lymphoid organs, induces functional inactivation of CD4(+) T-cells. Expressed by a distinct bone marrow-derived population, induces self-tolerance through a mechanism that does not require regulatory T-cells and is resitant to innate inflammatory stimuli. KEYWORDS: Activator;Alternative splicing;Cytoplasm;DNA-binding;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Repeat;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Cytoplasm Note=Predominantly nuclear but also cytoplasmic. Found in nuclear body-like structures (dots) and in a filamentous vimentin-like pattern. MISCELLANEOUS: [Isoform 3a]: Probably inactive.; MISCELLANEOUS: [Isoform 3b]: Probably inactive.; MISCELLANEOUS: [Isoform 3c]: Probably inactive.; MISCELLANEOUS: [Isoform 3d]: Probably inactive." Q9Z0F3,"PROTEIN NAMES: Bcl-2-like protein 10 (Bcl2-L-10) (Anti-apoptotic protein Boo) (Apoptosis regulator Bcl-B) (Bcl-2 homolog Diva) PROTEIN FAMILY: Bcl-2 family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the Bcl-2 family. FUNCTION: Promotes cell survival by suppressing apoptosis induced by BAX but not BAK (By similarity). Increases binding of AHCYL1/IRBIT to ITPR1 (By similarity). Reduces ITPR1-mediated calcium release from the endoplasmic reticulum cooperatively with AHCYL1/IRBIT under normal cellular conditions (By similarity). Under apoptotic stress conditions, dissociates from ITPR1 and is displaced from mitochondria-associated endoplasmic reticulum membranes, leading to increased Ca(2+) transfer to mitochondria which promotes apoptosis (By similarity). Required for the correct formation of the microtubule organizing center during oocyte cell division, potentially via regulation of protein abundance and localization of other microtubule organizing center components such as AURKA and TPX2. KEYWORDS: 3D-structure;Apoptosis;Cytoplasm;Cytoskeleton;Endoplasmic reticulum;Isopeptide bond;Membrane;Mitochondrion;Nucleus;Reference proteome;Transmembrane;Transmembrane helix;Ubl conjugation SUBCELLULAR LOCATION: Mitochondrion Nucleus membrane Endoplasmic reticulum Cytoplasm, cytoskeleton, spindle Note=Localizes to mitochondria-associated endoplasmic reticulum membranes (MAMs) (By similarity). Localization to MAMs is greatly reduced under apoptotic stress conditions (By similarity). " Q9Z123,"PROTEIN NAMES: Semaphorin-4F (Semaphorin-W) (Sema W) PROTEIN FAMILY: Semaphorin family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the semaphorin family. FUNCTION: Probable cell surface receptor that regulates oligodendroglial precursor cell migration. Might also regulate differentiation of oligodendroglial precursor cells (By similarity). Has growth cone collapse activity against retinal ganglion-cell axons (By similarity). KEYWORDS: Cell membrane;Cell projection;Developmental protein;Differentiation;Disulfide bond;Glycoprotein;Immunoglobulin domain;Membrane;Neurogenesis;Phosphoprotein;Reference proteome;Signal;Synapse;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein Postsynaptic density Perikaryon Cell projection, dendrite Note=Colocalizes with DLG4 at synapses. " Q9Z132,"PROTEIN NAMES: R-spondin-1 (Cysteine-rich and single thrombospondin domain-containing protein 3) (Cristin-3) (mCristin-3) (Roof plate-specific spondin-1) PROTEIN FAMILY: R-spondin family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the R-spondin family. FUNCTION: Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Has a essential roles in ovary determination (By similarity). Regulates Wnt signaling by antagonizing DKK1/KREM1-mediated internalization of LRP6 through an interaction with KREM1 (By similarity). KEYWORDS: Disulfide bond;Glycoprotein;Heparin-binding;Nucleus;Reference proteome;Repeat;Secreted;Sensory transduction;Signal;Wnt signaling pathway SUBCELLULAR LOCATION: Secreted Nucleus Note=Seems to mainly localize to nucleoli. " Q9Z138,"PROTEIN NAMES: Tyrosine-protein kinase transmembrane receptor ROR2 (mROR2) (Neurotrophic tyrosine kinase, receptor-related 2) PROTEIN FAMILY: Protein kinase superfamily, Tyr protein kinase family, ROR subfamily ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. ROR subfamily. FUNCTION: Tyrosine-protein kinase receptor which may be involved in the early formation of the chondrocytes. It seems to be required for cartilage and growth plate development. Phosphorylates YWHAB, leading to induction of osteogenesis and bone formation. In contrast, has also been shown to have very little tyrosine kinase activity in vitro. May act as a receptor for wnt ligand WNT5A which may result in the inhibition of WNT3A-mediated signaling (By similarity). KEYWORDS: ATP-binding;Cell membrane;Developmental protein;Disulfide bond;Glycoprotein;Immunoglobulin domain;Kinase;Kringle;Magnesium;Membrane;Metal-binding;Methylation;Nucleotide-binding;Phosphoprotein;Receptor;Reference proteome;Signal;Transferase;Transmembrane;Transmembrane helix;Wnt signaling pathway SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein " Q9Z1N6,PROTEIN NAMES: Secreted frizzled-related sequence protein 4 (FRP-4) (sFRP-4) PROTEIN FAMILY: Secreted frizzled-related protein (sFRP) family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the secreted frizzled-related protein (sFRP) family. FUNCTION: Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP4 plays a role in bone morphogenesis. May also act as a regulator of adult uterine morphology and function. May also increase apoptosis during ovulation possibly through modulation of FZ1/FZ4/WNT4 signaling (By similarity). Has phosphaturic effects by specifically inhibiting sodium-dependent phosphate uptake (By similarity). KEYWORDS: Developmental protein;Differentiation;Disulfide bond;Glycoprotein;Reference proteome;Secreted;Signal;Wnt signaling pathway SUBCELLULAR LOCATION: Secreted Q9Z1N7,PROTEIN NAMES: AT-rich interactive domain-containing protein 3B (ARID domain-containing protein 3B) (Bright and dead ringer protein) (Bright-like protein) ORGANISM: Mus musculus (Mouse) FUNCTION: Transcription factor involved in the production of cranial mesenchymal tissues. Favors nuclear targeting of ARID3A. KEYWORDS: Acetylation;Alternative splicing;Developmental protein;DNA-binding;Methylation;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus Q9Z1T5,"PROTEIN NAMES: Deformed epidermal autoregulatory factor 1 homolog (Nuclear DEAF-1-related transcriptional regulator) (NUDR) ORGANISM: Mus musculus (Mouse) FUNCTION: Transcription factor that binds to sequence with multiple copies of 5'-TTC[CG]G-3' present in its own promoter and that of the HNRPA2B1 gene. Down-regulates transcription of these genes. Binds to the retinoic acid response element (RARE) 5'-AGGGTTCACCGAAAGTTCA-3'. Activates the proenkephalin gene independently of promoter binding, probably through protein-protein interaction (By similarity). Regulates epithelial cell proliferation and side-branching in the mammary gland. Required for neural tube closure and skeletal patterning. Controls the expression of peripheral tissue antigens in pancreatic lymph nodes. Isoform 1 displays greater transcriptional activity than isoform 2. Isoform 2 may inhibit transcriptional activity of isoform 1 by interacting with it and retaining it in the cytoplasm. Transcriptional activator of EIF4G3 (By similarity). May also involved in behavior. KEYWORDS: 3D-structure;Alternative splicing;Cytoplasm;Developmental protein;DNA-binding;Metal-binding;Neurogenesis;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: [Isoform 1]: Nucleus. Cytoplasm.; SUBCELLULAR LOCATION: [Isoform 2]: Cytoplasm. Nucleus. Note=Displays some nuclear localization when expressed with isoform 1, suggesting that it may heterodimerize with isoform 1 and shuttle to the nucleus using the nuclear localization signal of isoform 1. " Q9Z204,"PROTEIN NAMES: Heterogeneous nuclear ribonucleoproteins C1/C2 (hnRNP C1/C2) PROTEIN FAMILY: RRM HNRPC family, RALY subfamily ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the RRM HNRPC family. RALY subfamily. FUNCTION: Binds pre-mRNA and nucleates the assembly of 40S hnRNP particles. Interacts with poly-U tracts in the 3'-UTR or 5'-UTR of mRNA and modulates the stability and the level of translation of bound mRNA molecules. Single HNRNPC tetramers bind 230-240 nucleotides. Trimers of HNRNPC tetramers bind 700 nucleotides. May play a role in the early steps of spliceosome assembly and pre-mRNA splicing. N6-methyladenosine (m6A) has been shown to alter the local structure in mRNAs and long non-coding RNAs (lncRNAs) via a mechanism named 'm(6)A-switch', facilitating binding of HNRNPC, leading to regulation of mRNA splicing. KEYWORDS: Acetylation;Alternative splicing;Coiled coil;Direct protein sequencing;Isopeptide bond;mRNA processing;mRNA splicing;Nucleus;Phosphoprotein;Reference proteome;Ribonucleoprotein;RNA-binding;Spliceosome;Ubl conjugation SUBCELLULAR LOCATION: Nucleus Note=Component of ribonucleosomes. " Q9Z214,"PROTEIN NAMES: Homer protein homolog 1 (PSD-Zip45) (VASP/Ena-related gene up-regulated during seizure and LTP 1) (Vesl-1) PROTEIN FAMILY: Homer family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the Homer family. FUNCTION: Postsynaptic density scaffolding protein. Binds and cross-links cytoplasmic regions of GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2, SHANK1 and SHANK3. By physically linking GRM1 and GRM5 with ER-associated ITPR1 receptors, it aids the coupling of surface receptors to intracellular calcium release. May also couple GRM1 to PI3 kinase through its interaction with AGAP2. Differentially regulates the functions of the calcium activated channel ryanodine receptors RYR1 and RYR2. Isoform 1 decreases the activity of RYR2, and increases the activity of RYR1, whereas isoform 3 counteracts the effects by competing for binding sites. Isoform 1 regulates the trafficking and surface expression of GRM5. Isoform 3 acts as a natural dominant negative, in dynamic competition with constitutively expressed isoform 1, and isoform 2 to regulate synaptic metabotropic glutamate function. Isoform 3, may be involved in the structural changes that occur at synapses during long-lasting neuronal plasticity and development. Forms a high-order complex with SHANK1, which in turn is necessary for the structural and functional integrity of dendritic spines. Negatively regulates T cell activation by inhibiting the calcineurin-NFAT pathway. Acts by competing with calcineurin/PPP3CA for NFAT protein binding, hence preventing NFAT activation by PPP3CA (By similarity). KEYWORDS: 3D-structure;Acetylation;Alternative splicing;Cell projection;Coiled coil;Cytoplasm;Phosphoprotein;Reference proteome;Synapse SUBCELLULAR LOCATION: Cytoplasm Postsynaptic density Synapse Cell projection, dendritic spine Note=Isoform 1 inhibits surface expression of GRM5 causing it to be retained in the endoplasmic reticulum. " Q9Z273,"PROTEIN NAMES: Tubby-related protein 1 (Tubby-like protein 1) PROTEIN FAMILY: TUB family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the TUB family. FUNCTION: Required for normal development of photoreceptor synapses. Required for normal photoreceptor function and for long-term survival of photoreceptor cells. Interacts with cytoskeleton proteins and may play a role in protein transport in photoreceptor cells. Binds lipids, especially phosphatidylinositol 3-phosphate, phosphatidylinositol 4-phosphate, phosphatidylinositol 5-phosphate, phosphatidylinositol 3,4-bisphosphate, phosphatidylinositol 4,5-bisphosphate, phosphatidylinositol 3,4,5-bisphosphate, phosphatidylserine and phosphatidic acid (in vitro) (By similarity). Contribute to stimulation of phagocytosis of apoptotic retinal pigment epithelium (RPE) cells and macrophages. KEYWORDS: Cell membrane;Cytoplasm;Membrane;Phagocytosis;Reference proteome;Secreted;Sensory transduction;Synapse;Vision SUBCELLULAR LOCATION: Cytoplasm. Cell membrane ; Peripheral membrane protein ; Cytoplasmic side Secreted. Synapse. Note=Detected at synapses between photoreceptor cells and second-order neurons. Does not have a cleavable signal peptide and is secreted by an alternative pathway. " Q9ZBU1,"PROTEIN NAMES: Alditol oxidase (AldO) (Xylitol oxidase) PROTEIN FAMILY: Oxygen-dependent FAD-linked oxidoreductase family ORGANISM: Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) SIMILARITY: Belongs to the oxygen-dependent FAD-linked oxidoreductase family. FUNCTION: Oxidase that performs selective oxidation of the terminal primary hydroxyl group of several alditols, with a reduction of O2 to H2O2. Shows highest activity on xylitol and D-sorbitol, and a poor efficiency with D-mannitol and L-threitol. KEYWORDS: 3D-structure;FAD;Flavoprotein;Oxidoreductase;Reference proteome " Q9ZGH1,"PROTEIN NAMES: dTDP-4-amino-4,6-dideoxy-D-glucose ammonia-lyase (dTDP-D-quinovose dehydrogenase) (dTDP-D-quinovose:S-adenosyl-L-methionine 1-oxidoreductase) PROTEIN FAMILY: Radical SAM superfamily, DesII family ORGANISM: Streptomyces venezuelae SIMILARITY: Belongs to the radical SAM superfamily. DesII family. FUNCTION: Involved in the biosynthesis of dTDP-alpha-D-desosamine, a sugar found in several bacterial macrolide antibiotics. Catalyzes the SAM-dependent deamination of dTDP-4-amino-4,6-deoxyglucose (dTDP-viosamine) to yield dTDP-3-keto-4,6-deoxyglucose. It can also catalyze the oxidative dehydrogenation of the non-physiological substrate dTDP-D-quinovose to dTDP-3-keto-6-deoxy-d-glucose. It can also deaminate dTDP-3-amino-3,6-deoxyglucose. KEYWORDS: 4Fe-4S;Antibiotic biosynthesis;Direct protein sequencing;Iron;Iron-sulfur;Lyase;Metal-binding;Oxidoreductase;S-adenosyl-L-methionine MISCELLANEOUS: The reaction starts by the transfer of an electron from the [4Fe-4S] cluster to S-adenosyl-L-methionine, spliting it into methionine and the radical 5-deoxyadenosin-5'-yl, which attacks the sugar substrate." Q9ZPY8,PROTEIN NAMES: Transcription factor ABA-INDUCIBLE bHLH-TYPE (AtAIB) (Basic helix-loop-helix protein 17) (AtbHLH17) (bHLH 17) (Transcription factor EN 35) (bHLH transcription factor bHLH017) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Transcription activator. Positively regulates abscisic acid (ABA) response. Confers drought tolerance and sensitivity to ABA. KEYWORDS: Abscisic acid signaling pathway;Activator;DNA-binding;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus Q9ZQ31,"PROTEIN NAMES: Serine/threonine-protein kinase STY13 (AtSTYPK) (Serine/threonine/tyrosine-protein kinase 13) PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. FUNCTION: Serine/threonine protein kinase that phosphorylates proteins on serine, threonine and tyrosine residues. KEYWORDS: ATP-binding;Kinase;Nucleotide-binding;Phosphoprotein;Reference proteome;Serine/threonine-protein kinase;Transferase;Tyrosine-protein kinase " Q9ZQ85,"PROTEIN NAMES: Myb family transcription factor EFM (MYB-domain transcription factor HHO4) (Protein EARLY FLOWERING MYB) (Protein HRS1 HOMOLOG 4) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Transcription factor acting as a flowering repressor, directly repressing FT expression in a dosage-dependent manner in the leaf vasculature. KEYWORDS: Coiled coil;DNA-binding;Flowering;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " Q9ZQC6,"PROTEIN NAMES: Beta-1,4-xylosyltransferase IRX9 (Protein IRREGULAR XYLEM 9) (Xylan xylosyltransferase IRX9) PROTEIN FAMILY: Glycosyltransferase 43 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the glycosyltransferase 43 family. FUNCTION: Involved in the synthesis of the hemicellulose glucuronoxylan, a major component of secondary cell walls. Xylan xylosyltransferase that acts cooperatively with IRX14 to achieve the successive addition of xylosyl residues during xylan backbone elongation. KEYWORDS: Cell wall biogenesis/degradation;Glycoprotein;Glycosyltransferase;Golgi apparatus;Membrane;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus membrane ; Single-pass type II membrane protein " Q9ZQP1,"PROTEIN NAMES: Phosphatidylglycerophosphate phosphatase PTPMT1 (Protein TYROSINE PHOSPHATASE LOCALIZED TO THE MITOCHONDRION 1) (Putative dual specificity protein phosphatase DSP8) PROTEIN FAMILY: Protein-tyrosine phosphatase family, Non-receptor class dual specificity subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily. FUNCTION: Exhibits phosphatidylglycerophosphate phosphatase activity. Involved in root growth and columella cells organization. May possess protein phosphatase activity (By similarity). PATHWAY: Phospholipid metabolism; phosphatidylglycerol biosynthesis; phosphatidylglycerol from CDP-diacylglycerol: step 2/2. KEYWORDS: Hydrolase;Lipid biosynthesis;Lipid metabolism;Phospholipid biosynthesis;Phospholipid metabolism;Protein phosphatase;Reference proteome " Q9ZS97,"PROTEIN NAMES: Iron-sulfur cluster assembly SufBD family protein ABCI8, chloroplastic (ABC transporter I family member 8) (ABC transporter ABCI.8) (AtABCI8) (Non-intrinsic ABC protein 1) (Protein ABC1) (Plastid sufB-like protein) (Protein LONG AFTER FAR-RED 6) PROTEIN FAMILY: Iron-sulfur cluster assembly SufBD family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the iron-sulfur cluster assembly SufBD family. FUNCTION: Involved in light signaling, probably by mediating the transport and correct distribution of protoporphyrin IX, a chlorophyll precursor, in response to far-red light. KEYWORDS: Chloroplast;Plastid;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast " Q9ZSA8,"PROTEIN NAMES: Protein DMR6-LIKE OXYGENASE 1 (2-oxoglutarate (2OG)-Fe(II) oxygenase-like protein DLO1) (Protein SENESCENCE-ASSOCIATED GENE 108) (Salicylate 3-hydroxylase DLO1) (S3H DLO1) (SA 3-hydroxylase DLO1) (Salicylic acid 3-hydroxylase DLO1) PROTEIN FAMILY: Iron/ascorbate-dependent oxidoreductase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the iron/ascorbate-dependent oxidoreductase family. FUNCTION: Converts salicylic acid (SA) to both 2,3-dihydroxybenzoic acid (2,3-DHBA) and 2,5-DHBA in vitro but only 2,3-DHBA in vivo. Component of a negative feedback regulation system of SA levels during senescence. Regulates both onset and progression of leaf senescence. Negative regulator of defense against Hyaloperonospora arabidopsidis.; FUNCTION: (Microbial infection) Confers susceptibility to the downy mildew pathogen Hyaloperonospora arabidopsidis. KEYWORDS: Dioxygenase;Iron;Metal-binding;NAD;Oxidoreductase;Plant defense;Reference proteome " Q9ZSD4,"PROTEIN NAMES: Syntaxin-121 (AtSYP121) (Protein SYNTAXIN OF PLANTS 121) (Syntaxin-related protein At-Syr1) PROTEIN FAMILY: Syntaxin family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the syntaxin family. FUNCTION: Vesicle trafficking protein that functions in the secretory pathway (By similarity). Together with SYP61, regulates the post-Golgi trafficking of the aquaporin PIP2-7 to the plasma membrane, thus modulating cell membrane water permeability. KEYWORDS: Acetylation;Alternative initiation;Cell membrane;Coiled coil;Golgi apparatus;Membrane;Protein transport;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane ; Single-pass type IV membrane protein Golgi apparatus, trans-Golgi network membrane ; Single-pass type IV membrane protein " Q9ZSK5,"PROTEIN NAMES: Zeatin O-glucosyltransferase (Trans-zeatin O-beta-D-glucosyltransferase) PROTEIN FAMILY: UDP-glycosyltransferase family ORGANISM: Phaseolus lunatus (Lima bean) (Phaseolus limensis) SIMILARITY: Belongs to the UDP-glycosyltransferase family. FUNCTION: May regulate active versus storage forms of cytokinins, and could have an impact on seed growth. Can also use UDP-xylose to catalyze the formation of O-xylosylzeatin but at much lower affinity. KEYWORDS: Glycosyltransferase;Transferase " Q9ZSY8,"PROTEIN NAMES: Auxin-responsive protein IAA27 (Auxin-induced protein 27) (Indoleacetic acid-induced protein 27) (Phytochrome-associated protein 2) PROTEIN FAMILY: Aux/IAA family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the Aux/IAA family. FUNCTION: Aux/IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations. Repression is thought to result from the interaction with auxin response factors (ARFs), proteins that bind to the auxin-responsive promoter element (AuxRE). Formation of heterodimers with ARF proteins may alter their ability to modulate early auxin response genes expression. KEYWORDS: 3D-structure;Auxin signaling pathway;Nucleus;Reference proteome;Repressor;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " Q9ZT42,"PROTEIN NAMES: E3 ubiquitin-protein ligase RHF2A (RING-H2 finger F2a) (RING-H2 zinc finger protein RHF2a) (RING-type E3 ubiquitin transferase RHF2A) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: E3 ubiquitin-protein ligase involved in the positive regulation of the gametogenesis progression. Required for the degradation of KRP6, a cyclin-dependent kinase inhibitor which accumulates during meiosis and blocks the progression of subsequent mitoses during gametophytes development. Functions in association with RHF1A. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Alternative splicing;Metal-binding;Reference proteome;Transferase;Ubl conjugation pathway;Zinc;Zinc-finger " Q9ZT92,"PROTEIN NAMES: RNA demethylase ALKBH10B (Alkylated DNA repair protein alkB homolog 10B) (Alpha-ketoglutarate-dependent dioxygenase alkB homolog 10B) PROTEIN FAMILY: AlkB family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the alkB family. FUNCTION: Dioxygenase that demethylates RNA by oxidative demethylation: specifically demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes. ALKBH10B-mediated mRNA m6A demethylation stabilizes the mRNA of the key flowering time regulators FT, SPL3 and SPL9, which are involved in the control of floral transition. KEYWORDS: Coiled coil;Dioxygenase;Iron;Metal-binding;Oxidoreductase;Reference proteome " Q9ZU46,"PROTEIN NAMES: Receptor protein kinase-like protein ZAR1 (Protein ZYGOTIC ARREST 1) PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. FUNCTION: Receptor protein kinase acting as an integrator for intracellular calcium and heterotrimeric G protein signaling with extracellular signals during early zygote development. Involved in modulating the asymmetric division of zygote and the cell fate determination of its daughter cells. Required for effector-triggered immunity (e.g. Pseudomonas syringae effector HopZ1a) via an activation triggered by SZE1, BKN2/SZE2 and ZED1, thus being essential for resistance against P. syringae pv. tomato DC3000 expressing HopZ1a. KEYWORDS: Cell membrane;Kinase;Leucine-rich repeat;Membrane;Phosphoprotein;Receptor;Reference proteome;Repeat;Signal;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass membrane protein " Q9ZUL3,"PROTEIN NAMES: Protein indeterminate-domain 5, chloroplastic (ID1-like zinc finger protein 2) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Transcription factor acting as a positive regulator of the starch synthase SS4. Controls chloroplast development and starch granule formation. Binds DNA via its zinc fingers. Recognizes and binds to SCL3 promoter sequence 5'-AGACAA-3' to promote its expression when in complex with RGA. KEYWORDS: Activator;Chloroplast;DNA-binding;Metal-binding;Phosphoprotein;Plastid;Reference proteome;Repeat;Transcription;Transcription regulation;Transit peptide;Zinc;Zinc-finger SUBCELLULAR LOCATION: Plastid, chloroplast " Q9ZUL5,PROTEIN NAMES: Protein CHROMATIN REMODELING 19 (AtCHR19) (AtRAD54-like protein) PROTEIN FAMILY: SNF2/RAD54 helicase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the SNF2/RAD54 helicase family. FUNCTION: DNA helicase that possesses intrinsic ATP-dependent nucleosome-remodeling activity and is both required for DNA repair and heterochromatin organization. Promotes DNA end resection of double-strand breaks (DSBs) following DNA damage: probably acts by weakening histone DNA interactions in nucleosomes flanking DSBs (By similarity). Probable chromatin remodeling factor. Probable helicase-like transcription factor involved in transcriptional gene silencing. Associates with SUVR2 and contributes to transcriptional gene silencing at RNA-directed DNA methylation (RdDM) target loci but also at RdDM-independent target loci. May be involved in nucleosome positioning to form ordered nucleosome arrays on chromatin. KEYWORDS: ATP-binding;Chromatin regulator;Coiled coil;DNA damage;DNA repair;DNA-binding;Helicase;Hydrolase;Nucleotide-binding;Nucleus;Reference proteome;RNA-mediated gene silencing;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: Induction by the mutagens rose Bengal (RB) and methyl methane sulfonate (MMS) is enhanced in progeny of Chernobyl plants exposed to ionizing radiation thus leading to a higher resistance to DNA damages. Q9ZUP3,"PROTEIN NAMES: Nucleosome assembly protein 1;2 (AtNAP1;2) (Nucleosome/chromatin assembly factor group A2) PROTEIN FAMILY: Nucleosome assembly protein (NAP) family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the nucleosome assembly protein (NAP) family. FUNCTION: May modulate chromatin structure by regulation of nucleosome assembly/disassembly (By similarity). May function in nucleotide excision repair (NER). Involved in somatic homologous recombination. KEYWORDS: Alternative splicing;Chaperone;Coiled coil;Cytoplasm;Lipoprotein;Methylation;Nucleus;Phosphoprotein;Prenylation;Reference proteome SUBCELLULAR LOCATION: Nucleus Cytoplasm Note=Predominantly located in cytoplasm. MISCELLANEOUS: Triple mutant nap1;1-nap1;2-nap1;3 has no obvious visible phenotype but exhibits hypersensitivity to DNA damage after UV-radiation, affected transcription of NER related genes , slight hypersensitive response to abscisic acid (ABA) in seedling growth and impaired somatic homologous recombination." Q9ZV04,"PROTEIN NAMES: Peroxidase 24 (Atperox P24) (ATP47) PROTEIN FAMILY: Peroxidase family, Classical plant (class III) peroxidase subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily. FUNCTION: Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue. KEYWORDS: Calcium;Disulfide bond;Glycoprotein;Heme;Hydrogen peroxide;Iron;Metal-binding;Oxidoreductase;Peroxidase;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted MISCELLANEOUS: There are 73 peroxidase genes in A.thaliana." Q9ZVF5,PROTEIN NAMES: WUSCHEL-related homeobox 6 (Protein PRETTY FEW SEEDS 2) PROTEIN FAMILY: WUS homeobox family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the WUS homeobox family. FUNCTION: Transcription factor that plays a central role in ovule patterning by regulating cell proliferation of the maternal integuments and differentiation of the maegaspore mother cell (MCC). Involved in AGAMOUS (AG) repression in leaves. KEYWORDS: Developmental protein;Differentiation;DNA-binding;Homeobox;Nucleus;Oogenesis;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus Q9ZVN2,"PROTEIN NAMES: Phytyl ester synthase 1, chloroplastic PROTEIN FAMILY: Diacylglycerol acyltransferase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the diacylglycerol acyltransferase family. FUNCTION: Acyltransferase involved in fatty acid phytyl ester synthesis in chloroplasts, a process required for the maintenance of the photosynthetic membrane integrity during abiotic stress and senescence. Exhibits phytyl ester synthesis and diacylglycerol acyltransferase activities with broad substrate specificities, and can employ acyl-CoAs, acyl carrier proteins, and galactolipids as acyl donors. KEYWORDS: Acyltransferase;Chloroplast;Plastid;Reference proteome;Stress response;Transferase;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast, plastoglobule " Q9ZVX2,"PROTEIN NAMES: Transcription factor ABORTED MICROSPORES (Basic helix-loop-helix protein 21) (AtbHLH21) (bHLH 21) (Transcription factor EN 48) (bHLH transcription factor bHLH021) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Transcription factor. Plays a crucial role in tapetum development. Required for male fertility and pollen differentiation, especially during the post-meiotic transcriptional regulation of microspore development within the developing anther. Binds E-box regions in the AHL16/TEK promoter. KEYWORDS: Developmental protein;DNA-binding;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " Q9ZW22,"PROTEIN NAMES: Methylsterol monooxygenase 2-1 (Sterol 4-alpha-methyl-oxidase 2) (AtSMO2) (Sterol 4-alpha-methyl-oxidase 2-1) PROTEIN FAMILY: Sterol desaturase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the sterol desaturase family. FUNCTION: Non-heme iron oxygenase involved in sterols biosynthesis by catalyzing the removal of the second methyl group at the C-4 position. 24-ethylidenelophenol and 24-ethyllophenol are the preferred substrates. Together with SMO2-2, required during embryogenesis, probably by maintaining sterols and auxin homeostasis. KEYWORDS: Alternative splicing;Endoplasmic reticulum;Iron;Lipid biosynthesis;Lipid metabolism;Membrane;Monooxygenase;Oxidoreductase;Reference proteome;Steroid biosynthesis;Steroid metabolism;Sterol biosynthesis;Sterol metabolism;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein MISCELLANEOUS: Requires a membrane-bound cytochrome b5 as an obligatory electron carrier from NAD(P)H to SMO." Q9ZW82,"PROTEIN NAMES: Pirin-like protein 2 (PIRIN2) (Pirin-like protein At2g43120) PROTEIN FAMILY: Pirin family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the pirin family. FUNCTION: Involved in susceptibility to the bacterial plant pathogen Ralstonia solanacearum. Stabilizes the xylem cysteine protease XCP2 by blocking its autolysis. KEYWORDS: Cytoplasm;Iron;Metal-binding;Nucleus;Plant defense;Reference proteome SUBCELLULAR LOCATION: Cytoplasm, cytosol Nucleus " Q9ZWA9,PROTEIN NAMES: 12S seed storage protein CRD (Cruciferin D) (Legumin-type globulin storage protein CRD) [Cleaved into: 12S seed storage protein CRD alpha chain (12S seed storage protein CRD acidic chain); 12S seed storage protein CRD beta chain (12S seed storage protein CRD basic chain)] PROTEIN FAMILY: 11S seed storage protein (globulins) family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the 11S seed storage protein (globulins) family. FUNCTION: Seed storage protein. KEYWORDS: Direct protein sequencing;Disulfide bond;Phosphoprotein;Reference proteome;Seed storage protein;Signal;Storage protein;Ubl conjugation;Vacuole SUBCELLULAR LOCATION: Protein storage vacuole R4ZCU1,"PROTEIN NAMES: Precursor protein UG [Cleaved into: U-actitoxin-Ugr1c (U-AITX-Ugr1c) (AnmTX Ugr 9a-3) (Ugr 9-3); U-actitoxin-Ugr1b (U-AITX-Ugr1b) (AnmTX Ugr 9a-2) (Ugr 9-2); Pi-actitoxin-Ugr1a (Pi-AITX-Ugr1a) (Pi-AnmTX Ugr 9a-1) (Ugr 9-1)] PROTEIN FAMILY: Sea anemone structural class 9a family ORGANISM: Urticina grebelnyi (Painted anemone) SIMILARITY: Belongs to the sea anemone structural class 9a family. FUNCTION: [Pi-actitoxin-Ugr1a]: Affects the ASIC3 channel (ACCN3) and produces analgesic effects. It produces a reversible inhibition effect on both the transient and the sustained current of human ASIC3 channels expressed in X.laevis oocytes. It completely blocks the transient component (IC(50)=10 uM) and partially (48%) inhibits the amplitude of the sustained component (IC(50)=1.44 uM). Using in vivo tests in mice, it reverses inflammatory and acid-induced pain.; FUNCTION: [U-actitoxin-Ugr1b]: Does not affect the ASIC3 channel. Does not cause lethality or paralysis of noble crayfish (A.astacus) at a dose of 1 mg/kg.; FUNCTION: [U-actitoxin-Ugr1c]: Does not affect the ASIC3 channel. Does not cause lethality or paralysis of noble crayfish (A.astacus) at a dose of 1 mg/kg. KEYWORDS: 3D-structure;Cleavage on pair of basic residues;Direct protein sequencing;Disulfide bond;Ion channel impairing toxin;Nematocyst;Neurotoxin;Proton-gated sodium channel impairing toxin;Secreted;Signal;Toxin SUBCELLULAR LOCATION: Secreted Nematocyst MISCELLANEOUS: [Pi-actitoxin-Ugr1a]: Negative results: shows neither agonistic nor antagonistic activity on ASIC1a/ASIC1, ASIC1b/ASIC1 and ASIC2a/ASIC2 at concentrations up to 50 uM as well as on the hKv1.3/KCNA3 channel at concentrations up to 1 uM. It does not cause lethality or paralysis of noble crayfish (A.astacus) at a dose of 1 mg/kg." S0E627,"PROTEIN NAMES: Geranylgeranyl pyrophosphate synthase 2 (GGPP synthase 2) (GGPPSase 2) (GGS2) ((2E,6E)-farnesyl diphosphate synthase) (Dimethylallyltranstransferase) (Farnesyl diphosphate synthase) (Farnesyltranstransferase) (Geranylgeranyl diphosphate synthase) (Geranyltranstransferase) (Gibberellin cluster GGPP synthase) PROTEIN FAMILY: FPP/GGPP synthase family ORGANISM: Gibberella fujikuroi (strain CBS 195.34 / IMI 58289 / NRRL A-6831) (Bakanae and foot rot disease fungus) (Fusarium fujikuroi) SIMILARITY: Belongs to the FPP/GGPP synthase family. FUNCTION: Geranylgeranyl pyrophosphate synthase; part of the gene cluster that mediates the biosynthesis of gibberellins (GAs), diterpenoids that may provide a selective advantage during infection of the preferred host plant, rice. Gibberellins (GAs) are diterpenoids and are synthesized via the mevalonate pathway. Biosynthesis of the major metabolite GA3 (gibberellic acid) from geranylgeranyl diphosphate (GGPP) requires 13 steps. The GGPP produced by the geranylgeranyl diphosphate synthase GGS2 is converted to ent-kaurene via ent-copalyldiphosphate in a two-step cyclization reaction performed by the bifunctional ent-copalyl diphosphate synthase/ent-kaurene synthase enzyme (CPS/KS). Ent-Kaurene is metabolized to GAs by a series of oxidation reactions catalyzed by cytochrome P450 monooxygenases. Cytochrome P450 monooxygenase P450-4 is an ent-kaurene oxidase that catalyzes the three oxidation steps between ent-kaurene and ent-kaurenoic acid. The highly multifunctional cytochrome P450 monooxygenase P450-1 then catalyzes four steps involving oxidation at two carbon atoms, in the main pathway from ent-kaurenoic acid to GA14 via GA12-aldehyde as well as producing kaurenolides and fujenoic acids as by-products. The cytochrome P450 monooxygenase P450-2 then converts GA14 to GA4 by removal of C-20. GA4 is further converted to GA7 by the GA4 desaturase DES via 1,2-desaturation before cytochrome P450 monooxygenase P450-3, a 13-hydroxylase, hydroxylates GA7 to GA3, the final product of the GA-biosynthetic pathway. PATHWAY: Plant hormone biosynthesis; gibberellin biosynthesis. KEYWORDS: Isoprene biosynthesis;Magnesium;Metal-binding;Reference proteome;Transferase " S4UX02,"PROTEIN NAMES: Ferruginol synthase (Cytochrome P450 76AH1) PROTEIN FAMILY: Cytochrome P450 family ORGANISM: Salvia miltiorrhiza (Chinese sage) SIMILARITY: Belongs to the cytochrome P450 family. FUNCTION: Cytochrome P450 enzyme (CYP) which catalyzes a unique two-electron oxidation cascade on abieta-8,11,13-triene to produce ferruginol, an intermediate in tanshinone biosynthesis. KEYWORDS: 3D-structure;Endoplasmic reticulum;Heme;Iron;Membrane;Metal-binding;Monooxygenase;NADP;Oxidoreductase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Single-pass membrane protein " U3KRF2,"PROTEIN NAMES: 3-dehydroquinate synthase, chloroplastic PROTEIN FAMILY: Sugar phosphate cyclases superfamily, Dehydroquinate synthase family ORGANISM: Actinidia chinensis var. chinensis (Chinese soft-hair kiwi) SIMILARITY: Belongs to the sugar phosphate cyclases superfamily. Dehydroquinate synthase family. FUNCTION: Catalyzes the second step in the shikimate pathway. PATHWAY: Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 2/7. KEYWORDS: 3D-structure;Amino-acid biosynthesis;Aromatic amino acid biosynthesis;Chloroplast;Lyase;Metal-binding;NAD;Plastid;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast " V6CIV8,"PROTEIN NAMES: Dual specificity protein phosphatase lip-1 (Lateral signaling-induced phosphatase 1) (Mitogen-activated protein kinase phosphatase lip-1) (MAP kinase phosphatase lip-1) PROTEIN FAMILY: Protein-tyrosine phosphatase family, Non-receptor class dual specificity subfamily ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily. FUNCTION: Dephosphorylates MAP kinase mpk-1. KEYWORDS: Alternative splicing;Cell cycle;Cell division;Cell membrane;Differentiation;Hydrolase;Meiosis;Membrane;Mitosis;Oogenesis;Protein phosphatase;Reference proteome SUBCELLULAR LOCATION: Cell membrane ; Peripheral membrane protein Note=Localizes in rod-like structures at the plasma membrane of pachytene germline cells. " V6F510,"PROTEIN NAMES: Magnetosome protein MamB (MM33.3) (Probable iron transporter MamB) PROTEIN FAMILY: Cation diffusion facilitator (CDF) transporter family ORGANISM: Magnetospirillum gryphiswaldense (strain DSM 6361 / JCM 21280 / NBRC 15271 / MSR-1) SIMILARITY: Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. FUNCTION: Plays a dual, essential role in magnetosome formation; required for magnetosome vesicle formation as well as biomineralization (Probable). Requires heterodimerization with MamM for stability. Probably binds and transports iron (Probable). One of 7 genes (mamLQBIEMO) able to induce magnetosome membrane biogenesis; coexpression of mamLQRBIEMO in a deletion of the 17 gene mamAB operon restores magnetosome vesicle formation but not magnetite biosynthesis. KEYWORDS: Biomineralization;Cell inner membrane;Cell membrane;Direct protein sequencing;Ion transport;Iron;Iron transport;Magnetosome;Membrane;Metal-binding;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell inner membrane ; Multi-pass membrane protein Magnetosome membrane ; Multi-pass membrane protein Note=Purified magnetosomes remain attached to each other. Tagged protein has several locations; most is in 1-3 dots in the cell, also seen as patchy membrane localization, while about 20% localizes with magnetosomes in a straight line running through the center of the cell. MISCELLANEOUS: This bacteria makes up to 60 cubo-octahedral magnetosomes of about 45 nm in diameter which contain membrane-bound crystals of magnetite (Fe(3)O(4)).; MISCELLANEOUS: Expression of just the minimal mamAB gene cluster (MGMSRv2__2365 to MGMSRv2__2381), including this gene, is sufficient to form a minimal magnetosome chain with small magnetite particles." V9VGU0,PROTEIN NAMES: Vicilin Pin k 2.0101 (7S globulin) (7S seed storage protein) (Allergen Pin k 2) (Vicilin-type seed storage protein) (allergen Pin k 2.0101) PROTEIN FAMILY: 7S seed storage protein family ORGANISM: Pinus koraiensis (Korean pine) SIMILARITY: Belongs to the 7S seed storage protein family. FUNCTION: Seed storage protein. KEYWORDS: 3D-structure;Allergen;Copper;Direct protein sequencing;Glycoprotein;Metal-binding;Seed storage protein;Signal;Storage protein W0TA43,"PROTEIN NAMES: Autophagy-related protein 13 PROTEIN FAMILY: ATG13 family, Fungi subfamily ORGANISM: Kluyveromyces marxianus (strain DMKU3-1042 / BCC 29191 / NBRC 104275) (Yeast) (Candida kefyr) SIMILARITY: Belongs to the ATG13 family. Fungi subfamily. FUNCTION: Activates the ATG1 kinase in a nutritional condition dependent manner through the TOR pathway, leading to autophagy. Involved in ATG9 and ATG23 cycling through the pre-autophagosomal structure (By similarity). Also involved in cytoplasm to vacuole transport (Cvt) and more specifically in Cvt vesicle formation. Seems to play a role in the switching machinery regulating the conversion between the Cvt pathway and autophagy (By similarity). Finally, ATG13 is also required for glycogen storage during stationary phase (By similarity). KEYWORDS: 3D-structure;Autophagy;Cytoplasm;Phosphoprotein;Protein transport;Transport SUBCELLULAR LOCATION: Cytoplasm Preautophagosomal structure MISCELLANEOUS: Kluyveromyces marxianus proteins are shorter in length and have a more ordered secondary structure than their S.cerevisiae counterparts, which might contribute to the superior thermotolerance and solubility. K.marxianus could be therefore useful as a new model organism for further elucidation of the molecular details of autophagy." W3VKA4,"PROTEIN NAMES: Lipase A (CalA) PROTEIN FAMILY: AB hydrolase superfamily, Lipase family ORGANISM: Moesziomyces aphidis (Pseudozyma aphidis) SIMILARITY: Belongs to the AB hydrolase superfamily. Lipase family. FUNCTION: Hydrolyzes triglycerides, with a preference for substrates with short-chain lengths (C4 to C8). Has the highest activity with tributyrin (C4), followed by tricaproin (C6) and tricaprylin (C8). Can also hydrolyze vinylacetate (C2) and triolein (C18), but with lower efficiency. Has no activity with tripalmitin (C16). KEYWORDS: 3D-structure;Disulfide bond;Hydrolase;Lipid degradation;Lipid metabolism;Secreted;Signal SUBCELLULAR LOCATION: Secreted " W5EP13,"PROTEIN NAMES: 2-carboxy-D-arabinitol-1-phosphatase PROTEIN FAMILY: Phosphoglycerate mutase family ORGANISM: Triticum aestivum (Wheat) SIMILARITY: Belongs to the phosphoglycerate mutase family. FUNCTION: Phosphoglycerate mutase-like protein lacking PGM activity, but having 2-carboxy-D-arabinitol 1-phosphate (CA1P) phosphatase activity. Can dephosphorylate the closely related compounds 2-carboxy-D-arabinitol 1,5-bisphosphate (CABP) and 2-carboxy-D-ribitol-1,5-bisphosphate(CRBP), and 2,3-diphosphoglycerate. Prevents the accumulation of D-glycero-2,3-pentodiulose-1,5-bisphosphate (PDBP) a potent inhibitor of ribulose-1,5-bisphosphate carboxylase (RuBisCO). PDBP is produced during the oxidation of ribulose-1,5-bisphosphate, the substrate of RuBisCO. KEYWORDS: Chloroplast;Hydrolase;Plastid;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast stroma " Q05037,"PROTEIN NAMES: Potassium voltage-gated channel subfamily A member 4 (BAK4) (Voltage-gated potassium channel subunit Kv1.4) PROTEIN FAMILY: Potassium channel family, A (Shaker) subfamily, Kv1.4/KCNA4 sub-subfamily ORGANISM: Bos taurus (Bovine) SIMILARITY: Belongs to the potassium channel family. A (Shaker) (TC 1.A.1.2) subfamily. Kv1.4/KCNA4 sub-subfamily. FUNCTION: Voltage-gated potassium channel that mediates transmembrane potassium transport in excitable membranes. Forms tetrameric potassium-selective channels through which potassium ions pass in accordance with their electrochemical gradient. The channel alternates between opened and closed conformations in response to the voltage difference across the membrane. Can form functional homotetrameric channels and heterotetrameric channels that contain variable proportions of KCNA1, KCNA2, KCNA4, KCNA5, and possibly other family members as well; channel properties depend on the type of alpha subunits that are part of the channel (By similarity). Channel properties are modulated by cytoplasmic beta subunits that regulate the subcellular location of the alpha subunits and promote rapid inactivation. In vivo, membranes probably contain a mixture of heteromeric potassium channel complexes, making it difficult to assign currents observed in intact tissues to any particular potassium channel family member. Homotetrameric KCNA4 forms a potassium channel that opens in response to membrane depolarization, followed by rapid spontaneous channel closure. Likewise, a heterotetrameric channel formed by KCNA1 and KCNA4 shows rapid inactivation (By similarity). KEYWORDS: Cell membrane;Cell projection;Glycoprotein;Ion channel;Ion transport;Membrane;Phosphoprotein;Potassium;Potassium channel;Potassium transport;Reference proteome;Transmembrane;Transmembrane helix;Transport;Voltage-gated channel SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Cell projection, axon " Q05123,"PROTEIN NAMES: Actin-like protein ARP9 (Chromatin structure-remodeling complex protein ARP9) (SWI/SNF complex component ARP9) PROTEIN FAMILY: Actin family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the actin family. FUNCTION: Component of the chromatin structure remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton. This subunit is involved in transcriptional regulation. Heterodimer of ARP9 and ARP7 functions with HMG box proteins to facilitate proper chromatin architecture. Heterodimer formation is necessary for assembly into RSC complex. Part of the SWI/SNF complex, an ATP-dependent chromatin remodeling complex, is required for the positive and negative regulation of gene expression of a large number of genes. It changes chromatin structure by altering DNA-histone contacts within a nucleosome, leading eventually to a change in nucleosome position, thus facilitating or repressing binding of gene-specific transcription factors. KEYWORDS: 3D-structure;Chromatin regulator;Direct protein sequencing;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus Note=Localizes to centromeric and flanking chromatin. Association with these loci is dependent on STH1. MISCELLANEOUS: Present with 1790 molecules/cell in log phase SD medium." Q05140,"PROTEIN NAMES: Clathrin coat assembly protein AP180 (91 kDa synaptosomal-associated protein) (Clathrin coat-associated protein AP180) PROTEIN FAMILY: PICALM/SNAP91 family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the PICALM/SNAP91 family. FUNCTION: Adaptins are components of the adapter complexes which link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration. Binding of AP180 to clathrin triskelia induces their assembly into 60-70 nm coats. KEYWORDS: Alternative splicing;Cell membrane;Coated pit;Direct protein sequencing;Glycoprotein;Membrane;Methylation;Phosphoprotein;Protein transport;Reference proteome;Transport SUBCELLULAR LOCATION: Cell membrane. Membrane, coated pit; Peripheral membrane protein; Cytoplasmic side. Note=Component of the coat surrounding the cytoplasmic face of coated vesicles in the plasma membrane. " Q05166,"PROTEIN NAMES: Nucleoporin ASM4 (Nuclear pore protein NUP59) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. Active directional transport is assured by both, a Phe-Gly (FG) repeat affinity gradient for these transport factors across the NPC and a transport cofactor concentration gradient across the nuclear envelope (GSP1 and GSP2 GTPases associated predominantly with GTP in the nucleus, with GDP in the cytoplasm). May have a mitosis control function (By similarity). KEYWORDS: 3D-structure;Cell cycle;Cell division;Coiled coil;Membrane;Mitosis;mRNA transport;Nuclear pore complex;Nucleus;Phosphoprotein;Protein transport;Reference proteome;Repeat;Translocation;Transport SUBCELLULAR LOCATION: Nucleus, nuclear pore complex Nucleus membrane; Peripheral membrane protein; Cytoplasmic side. Nucleus membrane; Peripheral membrane protein; Nucleoplasmic side. Note=Symmetric distribution. MISCELLANEOUS: Present with 2740 molecules/cell in log phase SD medium." Q05192,"PROTEIN NAMES: Nuclear hormone receptor FTZ-F1 beta (Nuclear hormone receptor HR39) (dHR39) (Nuclear receptor subfamily 5 group B member 1) PROTEIN FAMILY: Nuclear hormone receptor family, NR5 subfamily ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the nuclear hormone receptor family. NR5 subfamily. FUNCTION: Acts as a cofactor to fushi tarazu (ftz). Facilitates the binding of ftz to DNA. Binds the sequence element 5'-YCYYGGYCR-3' in the zebra element of ftz. Probably also functions as a receptor for a yet unknown ligand. KEYWORDS: Activator;Alternative splicing;DNA-binding;Metal-binding;Nucleus;Phosphoprotein;Receptor;Reference proteome;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus. " Q05209,"PROTEIN NAMES: Tyrosine-protein phosphatase non-receptor type 12 (PTP-PEST) (Protein-tyrosine phosphatase G1) (PTPG1) PROTEIN FAMILY: Protein-tyrosine phosphatase family, Non-receptor class 4 subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the protein-tyrosine phosphatase family. Non-receptor class 4 subfamily. FUNCTION: Dephosphorylates a range of proteins, and thereby regulates cellular signaling cascades. Dephosphorylates cellular tyrosine kinases, such as ERBB2 and PTK2B/PYK2, and thereby regulates signaling via ERBB2 and PTK2B/PYK2. Selectively dephosphorylates ERBB2 phosphorylated at 'Tyr-1112', 'Tyr-1196', and/or 'Tyr-1248'. KEYWORDS: 3D-structure;Acetylation;Alternative splicing;Cell junction;Cell projection;Cytoplasm;Direct protein sequencing;Disease variant;Hydrolase;Phosphoprotein;Protein phosphatase;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Cell junction, focal adhesion Cell projection, podosome Note=Partial translocation to focal adhesion sites may be mediated by interaction with SORBS2. " Q05319,"PROTEIN NAMES: Serine proteinase stubble (Protein stubble-stubbloid) [Cleaved into: Serine proteinase stubble non-catalytic chain; Serine proteinase stubble catalytic chain] PROTEIN FAMILY: Peptidase S1 family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the peptidase S1 family. FUNCTION: Hormone dependent protease required for epithelial morphogenesis, including the formation of bristles, legs, and wings. Has a dual function, detaches imaginal disk cells from extracellular matrices through its extracellular proteolytic domain and transmits an outside-to-inside signal to its intracellular domain to modify the cytoskeleton during morphogenesis. KEYWORDS: Disulfide bond;Glycoprotein;Hydrolase;Membrane;Protease;Reference proteome;Serine protease;Signal-anchor;Transmembrane;Transmembrane helix;Zymogen SUBCELLULAR LOCATION: Membrane; Single-pass type II membrane protein. " Q05521,"PROTEIN NAMES: Diacylglycerol pyrophosphate phosphatase 1 (DGPP phosphatase) (Lysophosphatidate phosphatase) (Phosphatidate phosphatase) PROTEIN FAMILY: PA-phosphatase related phosphoesterase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the PA-phosphatase related phosphoesterase family. FUNCTION: Catalyzes the dephosphorylation of diacylglycerol diphosphate (DGPP) to phosphatidate (PA) and the subsequent dephosphorylation of PA to diacylglycerol (DAG). Together with LPP1, regulates intracellular DGPP and PA levels, which are phospholipid molecules believed to play a signaling role in stress response. Can also use lysophosphatidic acid (LPA) and phosphatidylglycerophosphate as substrates. Substrate preference is DGPP > LPA > PA. Activity is independent of a divalent cation ion and insensitive to inhibition by N-ethylmaleimide. KEYWORDS: Hydrolase;Membrane;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix;Vacuole SUBCELLULAR LOCATION: Vacuole membrane ; Multi-pass membrane protein MISCELLANEOUS: Present with 3038 molecules/cell in log phase SD medium." Q05636,"PROTEIN NAMES: Exosome complex component RRP45 (Ribosomal RNA-processing protein 45) PROTEIN FAMILY: RNase PH family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the RNase PH family. FUNCTION: Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and cryptic unstable transcripts (CUTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and in RNA surveillance pathways, preventing translation of aberrant mRNAs. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. RRP45 is part of the hexameric ring of RNase PH domain-containing subunits proposed to form a central channel which threads RNA substrates for degradation. KEYWORDS: 3D-structure;Cytoplasm;Exosome;Nucleus;Reference proteome;RNA-binding;rRNA processing SUBCELLULAR LOCATION: Cytoplasm Nucleus, nucleolus MISCELLANEOUS: Present with 4800 molecules/cell in log phase SD medium." Q05652,"PROTEIN NAMES: Serine/threonine-protein kinase pelle PROTEIN FAMILY: Protein kinase superfamily, TKL Ser/Thr protein kinase family, Pelle subfamily ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily. FUNCTION: Plays an essential role in the Tl receptor signaling pathway that establishes embryonic dorsoventral polarity; the signal directs import of dl into ventral and ventrolateral nuclei, thereby establishing dorsoventral polarity. Tub recruits pll to the plasma membrane and protein-protein interaction activates pll. KEYWORDS: 3D-structure;ATP-binding;Cell membrane;Cytoplasm;Kinase;Membrane;Nucleotide-binding;Reference proteome;Serine/threonine-protein kinase;Transferase SUBCELLULAR LOCATION: Cell membrane Cytoplasm Note=Associates with the plasma membrane during interphase syncytial blastoderm embryos, more specifically at the membrane invaginations around the nuclei. " Q05777,"PROTEIN NAMES: Cell wall biogenesis protein NCW2 (New cell wall protein 2) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Cell wall biogenesis protein that participates in the organization of the beta-glucan assembly. Involved in the mechanism responsible for cell tolerance to polyhexamethylene biguanide (PHMB), an antifungal agent. KEYWORDS: Cell membrane;Cell wall biogenesis/degradation;Glycoprotein;GPI-anchor;Lipoprotein;Membrane;Reference proteome;Signal SUBCELLULAR LOCATION: Cell membrane ; Lipid-anchor, GPI-anchor MISCELLANEOUS: Present with 688 molecules/cell in log phase SD medium." Q05785,"PROTEIN NAMES: Epsin-2 PROTEIN FAMILY: Epsin family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the epsin family. FUNCTION: Binds to membranes enriched in phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2) and phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). Required for endocytosis and localization of actin. KEYWORDS: 3D-structure;Cytoplasm;Endocytosis;Isopeptide bond;Lipid-binding;Membrane;Phosphoprotein;Reference proteome;Repeat;Ubl conjugation SUBCELLULAR LOCATION: Cytoplasm. Membrane; Peripheral membrane protein. Note=Localizes in a punctate pattern. Found in the actin cortical patches, although the majority is located at the cell periphery. MISCELLANEOUS: Present with 1970 molecules/cell in log phase SD medium." Q05871,"PROTEIN NAMES: 3,2-trans-enoyl-CoA isomerase (Delta(3),Delta(2)-enoyl-CoA isomerase) (D3,D2-enoyl-CoA isomerase) (Dodecenoyl-CoA isomerase) PROTEIN FAMILY: Enoyl-CoA hydratase/isomerase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the enoyl-CoA hydratase/isomerase family. FUNCTION: Essential for the beta oxidation of unsaturated fatty acids. PATHWAY: Lipid metabolism; fatty acid beta-oxidation. KEYWORDS: 3D-structure;Fatty acid metabolism;Isomerase;Lipid metabolism;Peroxisome;Reference proteome SUBCELLULAR LOCATION: Peroxisome Note=This location is DCI1 dependent. " Q059Y8,"PROTEIN NAMES: E3 ubiquitin-protein ligase DCST1 (DC-STAMP domain-containing protein 1) (RING-type E3 ubiquitin transferase) ORGANISM: Mus musculus (Mouse) FUNCTION: E3 ubiquitin-protein ligase which mediates 'Lys-48'-linked ubiquitination of STAT2 and induces its proteasomal degradation thereby negatively regulating type-I-interferon signaling.; FUNCTION: Essential sperm cell-surface protein required for sperm-egg fusion and fertilization. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Cell membrane;Cytoplasmic vesicle;Glycoprotein;Immunity;Innate immunity;Membrane;Metal-binding;Reference proteome;Transferase;Transmembrane;Transmembrane helix;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Cytoplasmic vesicle, secretory vesicle, acrosome membrane ; Multi-pass membrane protein Note=Localizes in the anterior acrosome before the acrosome reaction and then translocates to the equatorial segment in acrosome-reacted sperm. " Q06003,PROTEIN NAMES: E3 ubiquitin-protein ligase goliath ORGANISM: Drosophila melanogaster (Fruit fly) FUNCTION: Endosomal E3 ubiquitin-protein ligase that regulates the recycling endosome pathway. May play an indirect role in regulation of gene expression during embryonic mesoderm formation. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Alternative splicing;Developmental protein;DNA-binding;Endosome;Membrane;Metal-binding;Reference proteome;Signal;Transcription;Transcription regulation;Transferase;Transmembrane;Transmembrane helix;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Endosome membrane ; Single-pass type I membrane protein Q06058,"PROTEIN NAMES: Seipin (Few lipid droplets protein 1) PROTEIN FAMILY: Seipin family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the seipin family. FUNCTION: Involved in lipid metabolism and lipid droplet (LD) morphology, number, and size. Facilitates initiation of LD formation, and ensures that vectorial budding of LDs from the ER is directed towards the cytoplasm. KEYWORDS: 3D-structure;Endoplasmic reticulum;Lipid metabolism;Membrane;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein Note=Concentrates at endoplasmic reticulum lipid droplet junctions. MISCELLANEOUS: Present with 846 molecules/cell in log phase SD medium." Q06063,"PROTEIN NAMES: tRNA-dihydrouridine(20a/20b) synthase [NAD(P)+] (mRNA-dihydrouridine synthase DUS4) (tRNA-dihydrouridine synthase 4) PROTEIN FAMILY: Dus family, Dus4 subfamily ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the Dus family. Dus4 subfamily. FUNCTION: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs. Specifically modifies U20a and U20b in cytoplasmic tRNAs. Also able to mediate dihydrouridylation of some mRNAs, thereby affecting their translation (By similarity). KEYWORDS: Flavoprotein;FMN;mRNA processing;NAD;NADP;Oxidoreductase;Reference proteome;tRNA processing " Q06067,"PROTEIN NAMES: Signal transduction histidine-protein kinase AtoS ORGANISM: Escherichia coli (strain K12) FUNCTION: Member of the two-component regulatory system AtoS/AtoC. In the presence of acetoacetate, AtoS/AtoC stimulates the expression of the atoDAEB operon, leading to short chain fatty acid catabolism and activation of the poly-(R)-3-hydroxybutyrate (cPHB) biosynthetic pathway. Also induces the operon in response to spermidine. Involved in the regulation of motility and chemotaxis, via transcriptional induction of the flagellar regulon. AtoS is a membrane-associated kinase that phosphorylates and activates AtoC in response to environmental signals. KEYWORDS: ATP-binding;Cell inner membrane;Cell membrane;Kinase;Membrane;Nucleotide-binding;Phosphoprotein;Reference proteome;Transferase;Transmembrane;Transmembrane helix;Two-component regulatory system SUBCELLULAR LOCATION: Cell inner membrane ; Multi-pass membrane protein " Q06115,"PROTEIN NAMES: Bile salt hydrolase/transferase (BSH/T) (Bile acid amine N-acyltransferase) (Bile salt hydrolase) (BSH) (Choloylglycine hydrolase) (Conjugated bile acid hydrolase) PROTEIN FAMILY: Peptidase C59 family ORGANISM: Lactiplantibacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1) (Lactobacillus plantarum) SIMILARITY: Belongs to the peptidase C59 family. FUNCTION: Possesses dual functions in bile acid metabolism (By similarity). Acts as a bile salt hydrolase that catalyzes the deconjugation of glycine- and taurine-linked bile salts, which occurs naturally in the intestines of animals, releasing amino acid residues and deconjugated bile salts (bile acids). Can hydrolyze the amide bond in the bile salts glycocholate (GCA), glycodeoxycholate (GDCA), glycochenodeoxycholate (GCDCA), taurocholate (TCA), taurodeoxycholate (TDCA) and taurochenodeoxycholate (TCDCA). Shows a preference for glycine-conjugated bile acids as substrates. Also acts as an amine N-acyltransferase that conjugates a wide variety of amino acids to conjugated and non-conjugated bile acids, thus producing bacterial bile acid amidates (BBAAs) - also named microbially conjugated bile acids (MCBAs) - in the gastrointestinal tract (By similarity). These BBAAs may facilitate communication between the microbiota and host through the activation of host ligand-activated transcription factors (By similarity). PATHWAY: Lipid metabolism; bile acid biosynthesis. KEYWORDS: Hydrolase;Lipid metabolism;Reference proteome;Transferase " Q06135,"PROTEIN NAMES: 1,3-beta-glucanosyltransferase GAS2 (Glycolipid-anchored surface protein 2) PROTEIN FAMILY: Glycosyl hydrolase 72 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the glycosyl hydrolase 72 family. FUNCTION: Splits internally a 1,3-beta-glucan molecule and transfers the newly generated reducing end (the donor) to the non-reducing end of another 1,3-beta-glucan molecule (the acceptor) forming a 1,3-beta linkage, resulting in the elongation of 1,3-beta-glucan chains in the cell wall. Involved in spore wall assembly. KEYWORDS: 3D-structure;Cell membrane;Cell wall biogenesis/degradation;Disulfide bond;Glycoprotein;GPI-anchor;Lipoprotein;Membrane;Reference proteome;Signal;Transferase SUBCELLULAR LOCATION: Cell membrane ; Lipid-anchor, GPI-anchor " Q06136,"PROTEIN NAMES: 3-ketodihydrosphingosine reductase (KDS reductase) (3-dehydrosphinganine reductase) (Follicular variant translocation protein 1) (FVT-1) (Short chain dehydrogenase/reductase family 35C member 1) PROTEIN FAMILY: Short-chain dehydrogenases/reductases (SDR) family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FUNCTION: Catalyzes the reduction of 3'-oxosphinganine (3-ketodihydrosphingosine/KDS) to sphinganine (dihydrosphingosine/DHS), the second step of de novo sphingolipid biosynthesis. PATHWAY: Lipid metabolism; sphingolipid metabolism. KEYWORDS: Alternative splicing;Chromosomal rearrangement;Disease variant;Endoplasmic reticulum;Lipid metabolism;Membrane;NADP;Nucleotide-binding;Oxidoreductase;Palmoplantar keratoderma;Proto-oncogene;Reference proteome;Signal;Sphingolipid metabolism;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein MISCELLANEOUS: Was identified as a potential cancer therapy target as its inhibition leads to proteotoxic stress in those cancer cells that overproduce its substrate 3-oxosphinganine." Q06142,"PROTEIN NAMES: Importin subunit beta-1 (Importin-95) (Karyopherin subunit beta-1) (Karyopherin-95) PROTEIN FAMILY: Importin beta family, Importin beta-1 subfamily ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the importin beta family. Importin beta-1 subfamily. FUNCTION: Importin beta subunit that functions in nuclear protein import through association with the importin alpha subunit, which binds to the classical nuclear localization signal (cNLS) in cargo substrates. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by importin beta through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, GTP-Ran binds to importin beta and the three components separate, leading to release of the cargo. Importin alpha and beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin beta. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates the nuclear import of histones H2A and H2B. Mediates the nuclear import of transcription factor GCN4. KEYWORDS: 3D-structure;Acetylation;Cytoplasm;Direct protein sequencing;mRNA transport;Nuclear pore complex;Nucleus;Phosphoprotein;Protein transport;Reference proteome;Repeat;Translocation;Transport SUBCELLULAR LOCATION: Cytoplasm Nucleus Nucleus, nuclear pore complex MISCELLANEOUS: Binds to nucleoporin FxFG but not GLFG repeat regions. Ran-GTP can disrupt the importin alpha/beta heterodimer by binding to the beta subunit and releases both subunits from the docking site.; MISCELLANEOUS: The stoichiometric complex between importin beta and Ran-GTP renders the latter inaccessible to Ran-specific GTPase activating protein (Ran-GAP) thereby inhibiting GTP hydrolysis stimulated by Ran-GAP.; MISCELLANEOUS: Present with 51700 molecules/cell in log phase SD medium." Q06147,"PROTEIN NAMES: Sphingoid long chain base kinase 5 (LCB kinase 5) (Sphinganine kinase 5) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Catalyzes the phosphorylation of the sphingoid long chain bases dihydrosphingosine (DHS or sphinganine) and phytosphingosine (PHS) to form dihydrosphingosine 1-phosphate (DHS-1P) and phytosphingosine 1-phosphate (PHS-1P) respectively. Redundant to LCB4, is only responsible for few percent of the total activity. Involved in the biosynthesis of sphingolipids and ceramides. Involved in heat-induced transient cell cycle arrest. Accumulation of phosphorylated sphingoid long chain bases (LCBPs) stimulates calcium influx and activates calcineurin signaling. Involved in heat-stress resistance. KEYWORDS: ATP-binding;Golgi apparatus;Kinase;Lipid metabolism;Lipoprotein;Membrane;Nucleotide-binding;Palmitate;Reference proteome;Sphingolipid metabolism;Transferase SUBCELLULAR LOCATION: Golgi apparatus membrane ; Peripheral membrane protein MISCELLANEOUS: Present with 1760 molecules/cell in log phase SD medium." Q06344,"PROTEIN NAMES: Pre-rRNA-processing protein ESF1 (18S rRNA factor 1) PROTEIN FAMILY: ESF1 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the ESF1 family. FUNCTION: Involved in the 18S rRNA synthesis. Required for the early cleavages at sites A0, A1 and A2. KEYWORDS: Coiled coil;Nucleus;Phosphoprotein;Reference proteome;Ribosome biogenesis;RNA-binding;rRNA processing SUBCELLULAR LOCATION: Nucleus, nucleolus MISCELLANEOUS: Present with 656 molecules/cell in log phase SD medium." Q06493,"PROTEIN NAMES: LETM1 domain-containing protein YLH47, mitochondrial (LETM1 homolog) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Involved in mitochondrial potassium homeostasis through the mitochondrial K(+)/H(+) exchange regulation. KEYWORDS: Coiled coil;Membrane;Mitochondrion;Mitochondrion inner membrane;Reference proteome;Transit peptide;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Mitochondrion inner membrane ; Single-pass membrane protein MISCELLANEOUS: Present with 11300 molecules/cell in log phase SD medium." Q06506,"PROTEIN NAMES: Ribosomal RNA-processing protein 9 PROTEIN FAMILY: WD repeat RRP9 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the WD repeat RRP9 family. FUNCTION: Involved in nucleolar processing of pre-18S ribosomal RNA. Required for efficient pre-rRNA cleavage at sites A0, A1 and A2, and biosynthesis of 18S rRNA. KEYWORDS: 3D-structure;Acetylation;Coiled coil;Nucleus;Phosphoprotein;Reference proteome;Repeat;Ribonucleoprotein;Ribosome biogenesis;rRNA processing;WD repeat SUBCELLULAR LOCATION: Nucleus, nucleolus MISCELLANEOUS: Present with 5130 molecules/cell in log phase SD medium." Q06508,"PROTEIN NAMES: Lysophosphatidic acid:oleoyl-CoA acyltransferase 1 (LPAAT) (Lysophosphatidic acid acyltransferase) (Vacuolar protein sorting-associated protein 66) PROTEIN FAMILY: 1-acyl-sn-glycerol-3-phosphate acyltransferase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family. FUNCTION: Acyl-CoA-dependent lysophosphatidic acid acyltransferase with preference for oleoyl-CoA. Involved in triacylglyceride homeostasis and lipid droplet formation. Involved in vacuolar protein sorting. KEYWORDS: Endoplasmic reticulum;Lipid droplet;Lipid metabolism;Membrane;Phospholipid metabolism;Reference proteome;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Lipid droplet Endoplasmic reticulum membrane ; Single-pass membrane protein Note=Lipid droplets consist of a surface phospholipid monolayer and a hydrophobic interior. The latter makes embedding of proteins containing transmembrane segments difficult, and these may instead adopt a hairpin or monotonic conformation when associated with lipid droplet membranes. Always localizes to lipid droplets, irrespective of whether cells are grown on glucose or oleate. MISCELLANEOUS: Present with 6630 molecules/cell in log phase SD medium." Q06510,"PROTEIN NAMES: Tafazzin (Taz) PROTEIN FAMILY: Taffazin family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the taffazin family. FUNCTION: Acyltransferase required to remodel newly synthesized phospholipid cardiolipin (1',3'-bis-[1,2-diacyl-sn-glycero-3-phospho]-glycerol or CL), a key component of the mitochondrial inner membrane, with tissue specific acyl chains necessary for adequate mitochondrial function. Its role in cellular physiology is to improve mitochondrial performance (By similarity). CL is critical for the coassembly of lipids and proteins in mitochondrial membranes, for instance, remodeling of the acyl groups of CL in the mitochondrial inner membrane affects the assembly and stability of respiratory chain complex IV and its supercomplex forms. Catalyzes the transacylation between phospholipids and lysophospholipids, with the highest rate being between phosphatidylcholine (1,2-diacyl-sn-glycero-3-phosphocholine or PC) and CL. Catalyzes both 1-acyl-sn-glycero-3-phosphocholine (lysophosphatidylcholine or LPC) reacylation and PC-CL transacylation, that means, it exchanges acyl groups between CL and PC by a combination of forward and reverse transacylations. Also catalyzes transacylations between other phospholipids such as phosphatidylethanolamine (1,2-diacyl-sn-glycero-3-phosphoethanolamine or PE) and CL, between PC and PE, and between PC and phosphatidate (1,2-diacyl-sn-glycero-3-phosphate or PA), although at lower rate. Not regiospecific, it transfers acyl groups into any of the sn-1 and sn-2 positions of the monolysocardiolipin (MLCL), which is an important prerequisite for uniformity and symmetry in CL acyl distribution. Cannot transacylate dilysocardiolipin (DLCL), thus, the role of MLCL is limited to that of an acyl acceptor (By similarity). CoA-independent, it can reshuffle molecular species within a single phospholipid class. Redistributes fatty acids between MLCL, CL, and other lipids, which prolongs the half-life of CL. Its action is completely reversible, which allows for cyclic changes, such as fission and fusion or bending and flattening of the membrane. Hence, by contributing to the flexibility of the lipid composition, it plays an important role in the dynamics of mitochondria membranes. Essential for the final stage of spermatogenesis, spermatid individualization (By similarity). Required for the initiation of mitophagy (By similarity). PATHWAY: Phospholipid metabolism. KEYWORDS: Acyltransferase;Lipid metabolism;Membrane;Mitochondrion;Mitochondrion inner membrane;Mitochondrion outer membrane;Reference proteome;Transferase SUBCELLULAR LOCATION: Mitochondrion outer membrane ; Peripheral membrane protein ; Intermembrane side Mitochondrion inner membrane ; Peripheral membrane protein ; Intermembrane side Note=Imported into mitochondria by the TOM complex and is first imported into the mitochondrion outer membrane in a TIM9-TIM10-dependent manner followed by insertion into the mitochondrion inner membrane. MISCELLANEOUS: Present with 1340 molecules/cell in log phase SD medium. The enzyme was named after a masochistic character Tafazzi, once popular on Italian television, apparently due to the difficulty encountered for its identification and characterization (By similarity)." Q06551,"PROTEIN NAMES: Palmitoyltransferase ERF2 (DHHC cysteine-rich domain-containing protein ERF2) (Ras protein acyltransferase) PROTEIN FAMILY: DHHC palmitoyltransferase family, ERF2/ZDHHC9 subfamily ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the DHHC palmitoyltransferase family. ERF2/ZDHHC9 subfamily. FUNCTION: The ERF2-SHR5 complex is a palmitoyltransferase specific for Ras proteins. Palmitoylates RAS2, which is required for its proper plasma membrane localization. KEYWORDS: 3D-structure;Acyltransferase;Endoplasmic reticulum;Lipoprotein;Membrane;Palmitate;Reference proteome;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein " Q06580,PROTEIN NAMES: Myosin light chain 2 (Calmodulin-like myosin light chain MLC2) (MYO1 light chain 2) (Myosin-1 light chain) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Regulatory light chain for the class II conventional myosin MYO1. May play a role in the disassembly of the MYO1 ring at the bud neck at the end of its contraction during cytokinesis. KEYWORDS: Calcium;Cell cycle;Cell division;Metal-binding;Motor protein;Myosin;Reference proteome;Repeat SUBCELLULAR LOCATION: Bud neck Note=Forms a ring at the bud neck in a MYO1-dependent manner. MISCELLANEOUS: Present with 1127 molecules/cell in log phase SD medium.; MISCELLANEOUS: This chain binds calcium. Q06593,PROTEIN NAMES: Oligopeptide transporter 2 PROTEIN FAMILY: Oligopeptide OPT transporter family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the oligopeptide OPT transporter family. FUNCTION: Transports tetra- and pentapeptides. Does not transport glutathione. KEYWORDS: Glycoprotein;Membrane;Peptide transport;Protein transport;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. Q06639,"PROTEIN NAMES: Chromatin structure-remodeling complex protein RSC3 (Remodel the structure of chromatin complex subunit 3) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Component of the chromatin structure-remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton. This subunit is required for transcription of ribosomal protein genes and genes involved in the integrity of the cell wall, and also for proper metaphase progression. Together with HTL1, LDB7, NPL6, RSC30 components, defines a fungal-specific module within the RSC complex that plays a role in many cellular functions including the maintenance of cell wall integrity. KEYWORDS: 3D-structure;Chromatin regulator;Direct protein sequencing;DNA-binding;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation;Zinc SUBCELLULAR LOCATION: Nucleus Note=Localizes to centromeric and flanking chromatin. Association with these loci is dependent on STH1. MISCELLANEOUS: Present with 1750 molecules/cell in log phase SD medium." Q06651,PROTEIN NAMES: E3 ubiquitin-protein ligase PIB1 (Phosphatidylinositol 3-phosphate-binding protein 1) (RING-type E3 ubiquitin transferase PIB1) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Functions as an E3 ubiquitin-protein ligase. Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Endosome;Membrane;Metal-binding;Reference proteome;Transferase;Ubl conjugation pathway;Vacuole;Zinc;Zinc-finger SUBCELLULAR LOCATION: Endosome membrane; Peripheral membrane protein. Vacuole membrane; Peripheral membrane protein. MISCELLANEOUS: Present with 195 molecules/cell in log phase SD medium. Q06679,"PROTEIN NAMES: U3 small nucleolar RNA-associated protein 4 (U3 snoRNA-associated protein 4) (U three protein 4) (U3 protein 4 required for transcription) (t-UTP4) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Involved in nucleolar processing of pre-18S ribosomal RNA. Required for optimal pre-ribosomal RNA transcription by RNA polymerase I together with a subset of U3 proteins required for transcription (t-UTPs). KEYWORDS: 3D-structure;Nucleus;Reference proteome;Repeat;Ribonucleoprotein;Ribosome biogenesis;rRNA processing;Transcription;WD repeat SUBCELLULAR LOCATION: Nucleus, nucleolus MISCELLANEOUS: Present with 5440 molecules/cell in log phase SD medium." Q06696,"PROTEIN NAMES: Vacuolar protein-sorting-associated protein 36 (ESCRT-II complex subunit VPS36) PROTEIN FAMILY: VPS36 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the VPS36 family. FUNCTION: Component of the ESCRT-II complex, which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. Involved in the trafficking of the plasma membrane ATPase. Its ability to bind ubiquitin plays a central role in endosomal sorting of ubiquitinated cargo proteins by the ESCRT complexes. KEYWORDS: 3D-structure;Cytoplasm;Endosome;Membrane;Metal-binding;Protein transport;Reference proteome;Repeat;Transport;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cytoplasm Endosome membrane ; Peripheral membrane protein MISCELLANEOUS: Present with 2470 molecules/cell in log phase SD medium." Q06697,"PROTEIN NAMES: Cell division control protein 73 (RNA polymerase-associated protein CDC73) PROTEIN FAMILY: CDC73 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the CDC73 family. FUNCTION: The PAF1 complex is a multifunctional complex. Involved in transcription initiation via genetic interactions with TATA-binding proteins. Involved in elongation. It regulates 3'-end formation of snR47 by modulating the recruitment or stable association of NRD1 and NAB3 with RNA polymerase II. Also has a role in transcription-coupled histone modification. Required for activation of RAD6 ubiquitin conjugate and the BRE1 ubiquitin ligase which ubiquitinate 'Lys-126' histone H2B. Activates the SET1 histone methyltransferase complex for methylation of 'Lys-4' of histone H3 and for methylation of 'Lys-73' of histone H3 by DOT1 and 'Lys-36' of histone H3 by SET2. KEYWORDS: 3D-structure;Activator;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus, nucleoplasm " Q06705,"PROTEIN NAMES: Phosphatidylinositol transfer protein CSR1 (CHS5 SPA2 rescue protein 1) (SEC14 homolog protein 2) PROTEIN FAMILY: PITP family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the PITP family. FUNCTION: Non-classical phosphatidylinositol (PtdIns) transfer protein (PITP), which exhibits PtdIns-binding/transfer activity in the absence of detectable PtdCho-binding/transfer activity. Activates SPO14/PLD1 (phospholipase D1) by stimulating phosphoinositide synthesis via the STT4 PtdIns 4-kinase. Modulates ArfGAP function through effects on SPO14 activity. Inhibits phosphatidylcholine degradation by PLB1 (phospholipase B1). May also regulate post-Golgi membrane-trafficking events and have a role resistance to oxidative stress. Inhibits fatty acid synthase activity in response to heme depletion and oleic acid starvation, preventing saturated fatty acid (SFA) accumulation. KEYWORDS: 3D-structure;Acetylation;Cytoplasm;Endoplasmic reticulum;Endosome;Lipid degradation;Lipid metabolism;Lipid transport;Microsome;Phospholipid degradation;Phospholipid metabolism;Phosphoprotein;Reference proteome;Transport SUBCELLULAR LOCATION: Cytoplasm Microsome Endosome MISCELLANEOUS: Present with 9600 molecules/cell in log phase SD medium." Q06945,"PROTEIN NAMES: Transcription factor SOX-4 ORGANISM: Homo sapiens (Human) FUNCTION: Transcriptional activator that binds with high affinity to the T-cell enhancer motif 5'-AACAAAG-3' motif. Required for IL17A-producing Vgamma2-positive gamma-delta T-cell maturation and development, via binding to regulator loci of RORC to modulate expression (By similarity). Involved in skeletal myoblast differentiation by promoting gene expression of CALD1. KEYWORDS: Acetylation;Activator;Disease variant;DNA-binding;Intellectual disability;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " Q06ZW3,"PROTEIN NAMES: Histone-lysine N-methyltransferase SETDB2 (SET domain bifurcated 2) PROTEIN FAMILY: Class V-like SAM-binding methyltransferase superfamily ORGANISM: Danio rerio (Zebrafish) (Brachydanio rerio) SIMILARITY: Belongs to the class V-like SAM-binding methyltransferase superfamily. FUNCTION: Histone methyltransferase involved in left-right axis specification in early development and mitosis. Specifically trimethylates 'Lys-9' of histone H3 (H3K9me3). H3K9me3 represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Contributes to H3K9me3 in both the interspersed repetitive elements and centromere-associated repeats. Plays a role in chromosome condensation and segregation during mitosis. During early development, required to specify the left-right axis by repressing expression of FGF8, leading to negatively regulate the dorsal organizer formation. KEYWORDS: Cell cycle;Cell division;Chromatin regulator;Chromosome;Developmental protein;Metal-binding;Methyltransferase;Mitosis;Nucleus;Reference proteome;S-adenosyl-L-methionine;Transferase;Zinc SUBCELLULAR LOCATION: Nucleus Chromosome " Q07071,"PROTEIN NAMES: Glucokinase regulatory protein (Glucokinase regulator) PROTEIN FAMILY: GCKR family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the GCKR family. FUNCTION: Regulates glucokinase (GCK) by forming an inactive complex with this enzyme. Acts by promoting GCK recruitment to the nucleus, possibly to provide a reserve of GCK that can be quickly released in the cytoplasm after a meal. The affinity of GCKR for GCK is modulated by fructose metabolites: GCKR with bound fructose 6-phosphate has increased affinity for GCK, while GCKR with bound fructose 1-phosphate has strongly decreased affinity for GCK and does not inhibit GCK activity. KEYWORDS: 3D-structure;Carbohydrate metabolism;Cytoplasm;Direct protein sequencing;Mitochondrion;Nucleus;Reference proteome;Repeat SUBCELLULAR LOCATION: Cytoplasm Nucleus Mitochondrion Note=Under low glucose concentrations, GCKR associates with GCK and the inactive complex is recruited to the hepatocyte nucleus. " Q07075,PROTEIN NAMES: Glutamyl aminopeptidase (EAP) (Aminopeptidase A) (AP-A) (Differentiation antigen gp160) (CD antigen CD249) PROTEIN FAMILY: Peptidase M1 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the peptidase M1 family. FUNCTION: Regulates central hypertension through its calcium-modulated preference to cleave N-terminal acidic residues from peptides such as angiotensin II. KEYWORDS: 3D-structure;Aminopeptidase;Calcium;Cell membrane;Direct protein sequencing;Disulfide bond;Glycoprotein;Hydrolase;Membrane;Metal-binding;Metalloprotease;Protease;Reference proteome;Signal-anchor;Transmembrane;Transmembrane helix;Zinc SUBCELLULAR LOCATION: Cell membrane ; Single-pass type II membrane protein. Q07084,"PROTEIN NAMES: Osmolarity two-component system protein SSK1 ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Final receptor of the SLN1-YPD1-SSK1 two-component regulatory system, which controls activity of the HOG1 pathway in response to changes in the osmolarity of the extracellular environment. Under normal osmotic conditions, maintained in a phosphorylated and inactive state by the phosphorelay intermediate protein YPD1. Under conditions of high osmolarity, the histidine kinase SLN1 is no longer active and the unphosphorylated form of SSK1 interacts with and activates SSK2 and SSK22, two MAPKKKs that further stimulate the PBS2-HOG1 MAPKK-MAPK cascade. Unphosphorylated SSK1 is subsequently degraded by the UBC7-dependent ubiquitin-proteasome system to down-regulate the HOG1 pathway after completion of the osmotic adaptation. KEYWORDS: 3D-structure;Cytoplasm;Phosphoprotein;Reference proteome;Two-component regulatory system SUBCELLULAR LOCATION: Cytoplasm MISCELLANEOUS: Present with 1200 molecules/cell in log phase SD medium." Q07104,"PROTEIN NAMES: Growth/differentiation factor 3 (GDF-3) (VG-1-related protein 2) PROTEIN FAMILY: TGF-beta family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the TGF-beta family. FUNCTION: Growth factor involved in early embryonic development and adipose-tissue homeostasis. During embryogenesis controls formation of anterior visceral endoderm and mesoderm and the establishment of anterior-posterior identity through a receptor complex comprising the receptor ACVR1B and the coreceptor CRIPTO. Regulates adipose-tissue homeostasis and energy balance under nutrient overload in part by signaling through the receptor complex based on ACVR1C and CRIPTO. KEYWORDS: Cytokine;Cytoplasm;Developmental protein;Disulfide bond;Glycoprotein;Growth factor;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted Cytoplasm Note=Mainly accumulated in the cytoplasm. MISCELLANEOUS: In contrast to other members of this family, cannot be disulfide-linked due to an atypical cysteine knot configuration, where the fourth cysteine is missing. This fourth cysteine is involved in an inter-molecular bridge to stabilize the active form of homodimeric or heterodimeric signaling molecules." Q07171,"PROTEIN NAMES: Gelsolin PROTEIN FAMILY: Villin/gelsolin family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the villin/gelsolin family. FUNCTION: Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. KEYWORDS: Actin-binding;Alternative splicing;Calcium;Cytoplasm;Cytoskeleton;Metal-binding;Phosphoprotein;Reference proteome;Repeat;Secreted;Signal SUBCELLULAR LOCATION: [Isoform 2]: Cytoplasm, cytoskeleton.; SUBCELLULAR LOCATION: [Isoform 1]: Secreted. " Q07192,"PROTEIN NAMES: Dual specificity mitogen-activated protein kinase kinase 2 (MAP kinase kinase 2) (MAPKK 2) (MAPK-ERK kinase 2) PROTEIN FAMILY: Protein kinase superfamily, STE Ser/Thr protein kinase family, MAP kinase kinase subfamily ORGANISM: Xenopus laevis (African clawed frog) SIMILARITY: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily. FUNCTION: Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. KEYWORDS: Alternative splicing;ATP-binding;Kinase;Nucleotide-binding;Phosphoprotein;Reference proteome;Serine/threonine-protein kinase;Transferase;Tyrosine-protein kinase " Q071E0,"PROTEIN NAMES: N-lysine methyltransferase KMT5A-A (Histone-lysine N-methyltransferase KMT5A-A) (Lysine-specific methylase 5A-A) (SET domain-containing protein 8-A) PROTEIN FAMILY: Class V-like SAM-binding methyltransferase superfamily, Histone-lysine methyltransferase family, PR/SET subfamily ORGANISM: Danio rerio (Zebrafish) (Brachydanio rerio) SIMILARITY: Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. PR/SET subfamily. FUNCTION: Protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins. Specifically monomethylates 'Lys-20' of histone H4 (H4K20me1). H4K20me1 is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. Required for cell proliferation, probably by contributing to the maintenance of proper higher-order structure of DNA during mitosis. Involved in chromosome condensation and proper cytokinesis. KEYWORDS: Cell cycle;Cell division;Chromatin regulator;Chromosome;Methyltransferase;Mitosis;Nucleus;Reference proteome;Repressor;S-adenosyl-L-methionine;Transcription;Transcription regulation;Transferase SUBCELLULAR LOCATION: Nucleus Chromosome Note=Specifically localizes to mitotic chromosomes. Associates with silent chromatin on euchromatic arms (By similarity). " Q07231,PROTEIN NAMES: Zinc finger and SCAN domain-containing protein 21 (CtFIN51) (Transcription factor RU49) (Zinc finger protein 38) (Zfp-38) PROTEIN FAMILY: Krueppel C2H2-type zinc-finger protein family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein family. FUNCTION: Strong transcriptional activator. Plays an important role in spermatogenesis; essential for the progression of meiotic prophase I in spermatocytes. KEYWORDS: Activator;Developmental protein;Differentiation;DNA-binding;Isopeptide bond;Meiosis;Metal-binding;Nucleus;Oogenesis;Reference proteome;Repeat;Spermatogenesis;Transcription;Transcription regulation;Ubl conjugation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Q07257,"PROTEIN NAMES: Transforming growth factor beta-2 proprotein [Cleaved into: Latency-associated peptide (LAP); Transforming growth factor beta-2 (TGF-beta-2)] PROTEIN FAMILY: TGF-beta family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the TGF-beta family. FUNCTION: [Transforming growth factor beta-2 proprotein]: Precursor of the Latency-associated peptide (LAP) and Transforming growth factor beta-2 (TGF-beta-2) chains, which constitute the regulatory and active subunit of TGF-beta-2, respectively.; FUNCTION: [Latency-associated peptide]: Required to maintain the Transforming growth factor beta-2 (TGF-beta-2) chain in a latent state during storage in extracellular matrix. Associates non-covalently with TGF-beta-2 and regulates its activation via interaction with 'milieu molecules', such as LTBP1 and LRRC32/GARP, that control activation of TGF-beta-2.; FUNCTION: [Transforming growth factor beta-2]: Multifunctional protein that regulates various processes such as angiogenesis and heart development (By similarity). Activation into mature form follows different steps: following cleavage of the proprotein in the Golgi apparatus, Latency-associated peptide (LAP) and Transforming growth factor beta-2 (TGF-beta-2) chains remain non-covalently linked rendering TGF-beta-2 inactive during storage in extracellular matrix (By similarity). At the same time, LAP chain interacts with 'milieu molecules', such as LTBP1 and LRRC32/GARP, that control activation of TGF-beta-2 and maintain it in a latent state during storage in extracellular milieus (By similarity). Once activated following release of LAP, TGF-beta-2 acts by binding to TGF-beta receptors (TGFBR1 and TGFBR2), which transduce signal (By similarity). KEYWORDS: Alternative splicing;Cleavage on pair of basic residues;Disulfide bond;Extracellular matrix;Glycoprotein;Growth factor;Mitogen;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: [Latency-associated peptide]: Secreted, extracellular space, extracellular matrix.; SUBCELLULAR LOCATION: [Transforming growth factor beta-2]: Secreted " Q07266,"PROTEIN NAMES: Drebrin (Developmentally-regulated brain protein) ORGANISM: Rattus norvegicus (Rat) FUNCTION: Actin cytoskeleton-organizing protein that plays a role in the formation of cell projections (By similarity). Required for actin polymerization at immunological synapses (IS) and for the recruitment of the chemokine receptor CXCR4 to IS (By similarity). Plays a role in dendritic spine morphogenesis and organization, including the localization of the dopamine receptor DRD1 to the dendritic spines (By similarity). Involved in memory-related synaptic plasticity in the hippocampus (By similarity). KEYWORDS: Acetylation;Actin-binding;Alternative splicing;Cell junction;Cell projection;Cytoplasm;Developmental protein;Differentiation;Neurogenesis;Phosphoprotein;Reference proteome;Ubl conjugation SUBCELLULAR LOCATION: Cytoplasm Cell projection, dendrite Cytoplasm, cell cortex Cell junction Cell projection Cell projection, growth cone Note=In the absence of antigen, evenly distributed throughout subcortical regions of the T-cell membrane and cytoplasm. In the presence of antigen, distributes to the immunological synapse forming at the T-cell-APC contact area, where it localizes at the peripheral and distal supramolecular activation clusters (SMAC). Colocalized with RUFY3 and F-actin at the transitional domain of the axonal growth cone. MISCELLANEOUS: Drebrins are classified into two forms of the embryonic type (E1 and E2) and one form of the adult type (A). The time course of their appearance are different from each other. Their structures are closely related. Adult rat brain expresses only drebrin A while drebrin E1 or E2 is observed in immature animals." Q07350,"PROTEIN NAMES: Pre-mRNA-splicing factor PRP11 PROTEIN FAMILY: SF3A2 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the SF3A2 family. FUNCTION: mRNA splicing factors, PRP9, PRP11, and PRP21, are necessary for addition of the U2 snRNP to the pre-mRNA in an early step of spliceosome assembly. KEYWORDS: 3D-structure;Metal-binding;mRNA processing;mRNA splicing;Nucleus;Reference proteome;Spliceosome;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: Present with 3460 molecules/cell in log phase SD medium." Q07412,PROTEIN NAMES: Triosephosphate isomerase (PfTIM) (Triose-phosphate isomerase) PROTEIN FAMILY: Triosephosphate isomerase family ORGANISM: Plasmodium falciparum SIMILARITY: Belongs to the triosephosphate isomerase family. FUNCTION: Catalyzes the interconversion of glyceraldehyde 3-phosphate and dihydroxyacetone phosphate in the glycolytic and gluconeogenic pathways. PATHWAY: Carbohydrate biosynthesis; gluconeogenesis.; PATHWAY: Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1. KEYWORDS: 3D-structure;Gluconeogenesis;Glycolysis;Isomerase Q07418,"PROTEIN NAMES: Peroxisomal membrane protein import receptor PEX19 (Peroxin-19) PROTEIN FAMILY: Peroxin-19 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the peroxin-19 family. FUNCTION: Required for proper post-translational import and stabilization of peroxisomal membrane proteins (PMPs). Acts as a cytosolic import receptor for PMPs and delivers them to the docking factor PEX3 at the peroxisomal membrane for subsequent insertion into the membrane. Acts as a chaperone in stabilizing or maintaining PMPs in the lipid bilayer. Directs PEX17, a peripheral component of the peroxisomal matrix protein translocation machinery, to peroxisomes. Stabilizes VPS1, a protein required for peroxisomal fission, at the peroxisomal membrane. Also acts in conjunction with PEX3 in the formation of peroxisomes from preperoxisomal compartments at the endoplasmic reticulum during de novo peroxisome synthesis, probably via the import of additional PMPs. KEYWORDS: Cytoplasm;Endoplasmic reticulum;Lipoprotein;Membrane;Methylation;Peroxisome;Peroxisome biogenesis;Phosphoprotein;Prenylation;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Peroxisome membrane ; Lipid-anchor ; Cytoplasmic side Endoplasmic reticulum membrane Note=Predominantly cytoplasmic. Concentrates in a PEX3-dependent manner to defined foci on the endoplasmic reticulum membrane, which then bud off to form newly sythesized peroxisomes. MISCELLANEOUS: Present with 5350 molecules/cell in log phase SD medium." Q07533,PROTEIN NAMES: Cytokinesis protein 3 PROTEIN FAMILY: CYK3 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the CYK3 family. FUNCTION: Involved in cytokinesis by recruiting INN1 to the bud neck. Cooperates with INN1 to stimulate the synthesis of the primary septum (PS) by CHS2. KEYWORDS: Cell cycle;Cell division;Cytoplasm;Phosphoprotein;Reference proteome;SH3 domain SUBCELLULAR LOCATION: Cytoplasm. Bud neck. Note=Found in association with the actin ring and the cortex at the mother-bud neck. MISCELLANEOUS: Present with 377 molecules/cell in log phase SD medium. Q07551,"PROTEIN NAMES: NADPH-dependent alpha-keto amide reductase (AKR-E) (YKAR) PROTEIN FAMILY: Aldo/keto reductase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the aldo/keto reductase family. FUNCTION: Reduces aromatic alpha-keto amides, aliphatic and aromatic alpha-keto esters, but not beta-keto esters. KEYWORDS: Cytoplasm;Direct protein sequencing;NADP;Nucleus;Oxidoreductase;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Nucleus MISCELLANEOUS: Present with 4030 molecules/cell in log phase SD medium." Q07560,PROTEIN NAMES: Cardiolipin synthase (CMP-forming) (CLS) PROTEIN FAMILY: CDP-alcohol phosphatidyltransferase class-I family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the CDP-alcohol phosphatidyltransferase class-I family. FUNCTION: Catalyzes the synthesis of cardiolipin (CL) (diphosphatidylglycerol) by specifically transferring a phosphatidyl group from CDP-diacylglycerol to phosphatidylglycerol (PG). CL is a key phospholipid in mitochondrial membranes and plays important roles in maintaining the functional integrity and dynamics of mitochondria under both optimal and stress conditions. KEYWORDS: Lipid biosynthesis;Lipid metabolism;Membrane;Mitochondrion;Mitochondrion inner membrane;Phospholipid biosynthesis;Phospholipid metabolism;Reference proteome;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Mitochondrion inner membrane; Multi-pass membrane protein. MISCELLANEOUS: Present with 876 molecules/cell in log phase SD medium. Q07657,"PROTEIN NAMES: Seventh homolog of septin 1 (Septation protein 7) PROTEIN FAMILY: TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily, Septin GTPase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family. FUNCTION: Septins are GTPases involved in cytokinesis that assemble early in the cell cycle as a patch at the incipient bud site and form a ring approximately 15 minutes before bud emergence, which transforms into an hour-glass shaped collar of cortical filaments that spans both sides of the mother-bud neck. This collar persists until just before cytokinesis, when it splits into two rings that occupy opposite sides of the neck. The septins at the bud neck serve as a structural scaffold that recruits different components involved in diverse processes at specific stages during the cell cycle. Many proteins bind asymmetrically to the septin collar. The septin assembly is regulated by protein kinases GIN4 and/or CLA4. May act by recruiting MYO1 and HOF1, a protein involved in septation, to the site of cleavage. Septins are also involved in cell morphogenesis, bud site selection, chitin deposition, cell cycle regulation, cell compartmentalization and spore wall formation. CDCd11 with SHS1 11 are involved in the recruitment of BNI5 and thereby ensure efficient localization at the bud neck of MYO1, the type II myosin of the actomyosin contractile ring. KEYWORDS: Acetylation;Cell cycle;Cell division;Coiled coil;GTP-binding;Isopeptide bond;Membrane;Nucleotide-binding;Phosphoprotein;Reference proteome;Ubl conjugation SUBCELLULAR LOCATION: Membrane ; Peripheral membrane protein Bud neck Note=Present at the bud neck during cell division. Probably interacts with phosphoinosides such as phosphatidylinositol 4-phosphate or phosphatidylinositol 5-phosphate. MISCELLANEOUS: Present with 5620 molecules/cell in log phase SD medium." Q07730,"PROTEIN NAMES: Extent of cell elongation protein 1 [Cleaved into: ECE1-I; ECE1-II; Candidalysin ECE1-III; ECE1-IV; ECE1-V; ECE1-VI; ECE1-VII; ECE1-VIII] ORGANISM: Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) FUNCTION: Secreted protein cleaved by KEX2 in 8 similar peptides (ECE1-I to ECE1-VIII). Stimulates biofilm formation.; FUNCTION: [Candidalysin ECE1-III]: Acts as a cytolytic peptide toxin that directly damages host epithelial membranes, triggers a danger response signaling pathway and activates epithelial immunity. Polymerizes in solution to form membrane pores to damage epithelial cells. Induces calcium influx, oxidative stress, mitochondrial dysfunction and ATP depletion in host cells, leading to epithelial necrosis. Serves as a danger signal that potentiates the immune response, and more specifically IL-17 response. Induces cytokine/chemokine secretion by host (especially CCL2/3/4, CXCL1 and S100A8), neutrophil recruitment, and promotes mortality in zebrafish and murine models of systemic fungal infection. Mediates distinct epithelial inflammatory responses through p38, EGFR-ERK and TREM-1/DAP12 pathways. Acts as one of the hypha-derived drivers of NLRP3 inflammasome responses in primary macrophages and thus contributes to the capacity to induce maturation and secretion of IL-1beta from primary macrophages. Stimulates mast cells by mediating cross-talk between signaling pathways activated by the dectin-1 receptor and MAPKs. Enables escape via the gasdermin-mediated pyroptosis, as well as a cell lysis pathway associated with macrophage extracellular trap formation termed ETosis. Acts as the main hemolytic factor of C.albicans. As an exotoxine, promotes also alcohol-associated liver disease or oral carcinogenesis. KEYWORDS: Cleavage on pair of basic residues;Host cell membrane;Host membrane;Membrane;Reference proteome;Repeat;Secreted;Signal;Toxin;Transmembrane;Transmembrane helix;Virulence SUBCELLULAR LOCATION: Secreted.; SUBCELLULAR LOCATION: [Candidalysin ECE1-III]: Host cell membrane ; Single-pass membrane protein Note=Candidalysin is delivered to the invasion pocket in which the hypha is tightly surrounded by the host membrane, to enable the full damage potential of during mucosal infection. " Q07782,"PROTEIN NAMES: Solute carrier family 13 member 1 (NaSi-1) (Renal sodium/sulfate cotransporter) (Na(+)/sulfate cotransporter) PROTEIN FAMILY: SLC13A/DASS transporter family, NADC subfamily ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the SLC13A/DASS transporter (TC 2.A.47) family. NADC subfamily. FUNCTION: Sodium:sulfate symporter that mediates sulfate reabsorption in the kidney and small intestine. Can also mediate the transport of selenate and thiosulfate. KEYWORDS: Cell membrane;Glycoprotein;Ion transport;Membrane;Reference proteome;Sodium;Sodium transport;Sulfate transport;Symport;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Apical cell membrane ; Multi-pass membrane protein " Q07821,PROTEIN NAMES: Iron-sulfur assembly protein 1 PROTEIN FAMILY: HesB/IscA family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the HesB/IscA family. FUNCTION: Involved in the assembly of mitochondrial and cytoplasmic iron-sulfur proteins. Probably involved in the binding of an intermediate of Fe/S cluster assembly. KEYWORDS: Iron;Metal-binding;Mitochondrion;Reference proteome SUBCELLULAR LOCATION: Mitochondrion matrix. MISCELLANEOUS: Present with 125 molecules/cell in log phase SD medium. Q07837,"PROTEIN NAMES: Amino acid transporter heavy chain SLC3A1 (D2h) (Neutral and basic amino acid transport protein) (NBAT) (Solute carrier family 3 member 1) (b(0,+)-type amino acid transporter-related heavy chain) (rBAT) ORGANISM: Homo sapiens (Human) FUNCTION: Acts as a chaperone that facilitates biogenesis and trafficking of functional transporter heteromers to the plasma membrane (By similarity). Associates with SLC7A9 to form a functional transporter complex that mediates the electrogenic exchange between cationic amino acids and neutral amino acids, with a stoichiometry of 1:1. SLC7A9-SLC3A1 transporter has system b(0,+)-like activity with high affinity for extracellular cationic amino acids and L-cystine and lower affinity for intracellular neutral amino acids. Substrate exchange is driven by high concentration of intracellular neutral amino acids and the intracellular reduction of L-cystine to L-cysteine. SLC7A9-SLC3A1 acts as a major transporter for reabsorption of L-cystine and dibasic amino acids across the brush border membrane in early proximal tubules. Associates with SLC7A13 to form a functional complex that transports anionic and neutral amino acids via exchange or facilitated diffusion. SLC7A13-SLC3A1 may act as a major transporter for L-cystine in late proximal tubules, ensuring its reabsorption from the luminal fluid in exchange for cytosolic L-glutamate or L-aspartate (By similarity). KEYWORDS: 3D-structure;Alternative splicing;Amino-acid transport;Cell membrane;Cystinuria;Disease variant;Disulfide bond;Glycoprotein;Membrane;Phosphoprotein;Reference proteome;Signal-anchor;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane ; Single-pass type II membrane protein Apical cell membrane ; Single-pass type II membrane protein " Q07896,"PROTEIN NAMES: Nucleolar complex-associated protein 3 PROTEIN FAMILY: CBF/MAK21 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the CBF/MAK21 family. FUNCTION: Required for synthesis of 60S ribosomal subunits and the transport of pre-ribosomes from the nucleoplasm to the cytoplasm. Also required for initiation of DNA replication. May function downstream of the origin recognition complex (ORC complex) in the loading of CDC6 and the minichromosome maintenance complex (MCM complex) onto chromatin during the G1 phase of the cell cycle. Essential for growth. KEYWORDS: 3D-structure;Cell cycle;Cell division;Coiled coil;DNA replication;Nucleus;Phosphoprotein;Reference proteome;Ribosome biogenesis SUBCELLULAR LOCATION: Nucleus, nucleolus MISCELLANEOUS: Present with 11700 molecules/cell in log phase SD medium." Q07913,PROTEIN NAMES: Non-structural maintenance of chromosomes element 1 (Non-SMC element 1) PROTEIN FAMILY: NSE1 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the NSE1 family. FUNCTION: Acts in a DNA repair pathway for removal of UV-induced DNA damage that is distinct from classical nucleotide excision repair and in repair of ionizing radiation damage. Functions in homologous recombination repair of DNA double strand breaks and in recovery of stalled replication forks. KEYWORDS: 3D-structure;DNA damage;DNA recombination;DNA repair;Metal-binding;Nucleus;Reference proteome;Transferase;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Q07950,"PROTEIN NAMES: Sterol esterase 2 (Steryl ester hydrolase 2) PROTEIN FAMILY: AB hydrolase superfamily ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the AB hydrolase superfamily. FUNCTION: Mediates the hydrolysis of steryl esters. Required for mobilization of steryl ester, thereby playing a central role in lipid metabolism. KEYWORDS: Cell membrane;Hydrolase;Lipid degradation;Lipid metabolism;Membrane;Phosphoprotein;Reference proteome;Signal-anchor;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type II membrane protein MISCELLANEOUS: Present with 1630 molecules/cell in log phase SD medium." Q08002,"PROTEIN NAMES: Neutral metalloprotease ShpI PROTEIN FAMILY: Peptidase M30 family ORGANISM: Staphylococcus hyicus SIMILARITY: Belongs to the peptidase M30 family. FUNCTION: Protease that has a low substrate specificity. Catalyzes the hydrolysis of glucagon, melittin and oxidized beta-insulin at various positions in vitro. Is not able to cleave elastin or the synthetic substrates FAGLA (a substrate for neutral proteinases) and FALGPA (a substrate for collagenase). KEYWORDS: Direct protein sequencing;Hydrolase;Metal-binding;Metalloprotease;Protease;Secreted;Signal;Zinc;Zymogen SUBCELLULAR LOCATION: Secreted " Q08004,PROTEIN NAMES: Bud site selection protein 20 PROTEIN FAMILY: ZNF593/BUD20 C2H2-type zinc-finger protein family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the ZNF593/BUD20 C2H2-type zinc-finger protein family. FUNCTION: Involved in pre-60S ribosomal particles maturation by promoting the nuclear export of the 60S ribosome. Involved in positioning the proximal bud pole signal. KEYWORDS: 3D-structure;Cytoplasm;Metal-binding;Nucleus;Reference proteome;Ribosome biogenesis;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Cytoplasm Note=Shuttles between the nucleus and the cytoplasm. MISCELLANEOUS: Present with 5630 molecules/cell in log phase SD medium. Q08050,"PROTEIN NAMES: Forkhead box protein M1 (Forkhead-related protein FKHL16) (Hepatocyte nuclear factor 3 forkhead homolog 11) (HFH-11) (HNF-3/fork-head homolog 11) (M-phase phosphoprotein 2) (MPM-2 reactive phosphoprotein 2) (Transcription factor Trident) (Winged-helix factor from INS-1 cells) ORGANISM: Homo sapiens (Human) FUNCTION: Transcription factor regulating the expression of cell cycle genes essential for DNA replication and mitosis. Plays a role in the control of cell proliferation. Also plays a role in DNA break repair, participating in the DNA damage checkpoint response. Promotes transcription of PHB2. KEYWORDS: 3D-structure;Activator;Alternative splicing;Cell cycle;DNA damage;DNA repair;DNA-binding;Isopeptide bond;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation;Ubl conjugation SUBCELLULAR LOCATION: Nucleus. " Q08096,"PROTEIN NAMES: RNA 3'-terminal phosphate cyclase-like protein PROTEIN FAMILY: RNA 3'-terminal cyclase family, Type 2 subfamily ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the RNA 3'-terminal cyclase family. Type 2 subfamily. FUNCTION: Does not have cyclase activity. Plays a role in 40S-ribosomal-subunit biogenesis in the early pre-rRNA processing steps at sites A0, A1 and A2 that are required for proper maturation of the 18S RNA. Essential for viability. KEYWORDS: 3D-structure;Acetylation;Nucleus;Reference proteome;Ribosome biogenesis SUBCELLULAR LOCATION: Nucleus, nucleolus. MISCELLANEOUS: Present with 10000 molecules/cell in log phase SD medium." Q08109,"PROTEIN NAMES: ERAD-associated E3 ubiquitin-protein ligase HRD1 (HMG-CoA reductase degradation protein 1) (RING-type E3 ubiquitin transferase HRD1) PROTEIN FAMILY: HRD1 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the HRD1 family. FUNCTION: E3 ubiquitin-protein ligase which accepts ubiquitin specifically from endoplasmic reticulum-associated UBC1 and UBC7 E2 ligases, and transfers it to substrates promoting their degradation. Mediates the degradation of endoplasmic reticulum proteins (ERQC), also called ER-associated degradation (ERAD). Component of the HRD1 ubiquitin ligase complex, which is part of the ERAD-L and ERAD-M pathways responsible for the rapid degradation of soluble lumenal and membrane proteins with misfolded lumenal domains (ERAD-L), or ER-membrane proteins with misfolded transmembrane domains (ERAD-M). In ERAD-L, facilitates retrotranslocation of misfolded proteins from the ER lumen through the ER membrane in conjunction with DER1. Both proteins have lateral gates facing each other which form a channel through the ER membrane and which distort the membrane region between the lateral gates, making it much thinner than a normal phospholipid bilayer. Substrates insert into the membrane as a hairpin loop with one strand interacting with DER1 and the other with HRD1. ERAD-L substrates are ubiquitinated through HRD1 in conjunction with the E2 ubiquitin-conjugating enzymes UBC1 and UBC7-CUE1. Ubiquitinated substrates are then removed to the cytosol via the action of the CDC48-NPL4-UFD1 ATPase complex and targeted to the proteasome. ERAD-M substrates are processed by the same HRD1-HRD3 core complex, but only a subset of the other components is required for ERAD-M. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: 3D-structure;Endoplasmic reticulum;Membrane;Metal-binding;Reference proteome;Transferase;Transmembrane;Transmembrane helix;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein MISCELLANEOUS: Present with 2660 molecules/cell in log phase SD medium." Q08144,"PROTEIN NAMES: t-SNARE affecting a late Golgi compartment protein 2 (Syntaxin TLG2) PROTEIN FAMILY: Syntaxin family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the syntaxin family. FUNCTION: t-SNARE that functions in transport from the endosome to the late Golgi and on the endocytic pathway. KEYWORDS: Coiled coil;Endosome;Golgi apparatus;Membrane;Phosphoprotein;Protein transport;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Golgi apparatus, trans-Golgi network membrane; Single-pass type IV membrane protein. Endosome membrane; Single-pass type IV membrane protein. " Q08169,PROTEIN NAMES: Hyaluronidase (Hya) (Allergen Api m II) (Hyaluronoglucosaminidase) (allergen Api m 2) PROTEIN FAMILY: Glycosyl hydrolase 56 family ORGANISM: Apis mellifera (Honeybee) SIMILARITY: Belongs to the glycosyl hydrolase 56 family. FUNCTION: Hydrolyzes high molecular weight hyaluronic acid to produce small oligosaccharides. KEYWORDS: 3D-structure;Allergen;Direct protein sequencing;Disulfide bond;Glycoprotein;Glycosidase;Hydrolase;Reference proteome;Secreted;Signal;Zymogen SUBCELLULAR LOCATION: Secreted. Q08179,"PROTEIN NAMES: Mitochondrial distribution and morphology protein 38 ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Involved in mitochondrial potassium homeostasis through the mitochondrial K(+)/H(+) exchange regulation. With MBA1, plays a role in ribosomal translation and protein insertion into the inner membrane. KEYWORDS: 3D-structure;Coiled coil;Membrane;Mitochondrion;Mitochondrion inner membrane;Reference proteome;Transit peptide;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Mitochondrion inner membrane ; Single-pass membrane protein MISCELLANEOUS: Present with 7390 molecules/cell in log phase SD medium." Q08180,PROTEIN NAMES: Irregular chiasm C-roughest protein (Protein IRREC) ORGANISM: Drosophila melanogaster (Fruit fly) FUNCTION: Required for correct axonal pathway formation in the optic lobe and for programmed cell death in the developing retina. KEYWORDS: 3D-structure;Cell adhesion;Disulfide bond;Glycoprotein;Immunoglobulin domain;Membrane;Reference proteome;Repeat;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Single-pass type I membrane protein Q08214,"PROTEIN NAMES: Endonuclease III homolog 2 (Bifunctional DNA N-glycosylase/DNA-(apurinic or apyrimidinic site) lyase 2) (DNA glycosylase/AP lyase 2) (Endonuclease III-like glycosylase 2) (Redoxyendonuclease 2) PROTEIN FAMILY: Nth/MutY family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the Nth/MutY family. FUNCTION: Bifunctional DNA N-glycosylase with associated apurinic/apyrimidinic (AP) lyase function that catalyzes the first step in base excision repair (BER), the primary repair pathway for the repair of oxidative DNA damage. The DNA N-glycosylase activity releases the damaged DNA base from DNA by cleaving the N-glycosidic bond, leaving an AP site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination. Primarily recognizes and repairs oxidative base damage of pyrimidines, but also purine-derived lesions, alkylation damage as well as abasic sites. Can also repair the oxidation products of 8-oxoguanine. KEYWORDS: 3D-structure;4Fe-4S;DNA damage;DNA repair;Glycosidase;Hydrolase;Iron;Iron-sulfur;Isopeptide bond;Lyase;Metal-binding;Nucleus;Reference proteome;Ubl conjugation SUBCELLULAR LOCATION: Nucleus Note=Exclusively nuclear and not responsive to changes in either nuclear or mitochondrial oxidative stress. MISCELLANEOUS: Present with 125 molecules/cell in log phase SD medium." Q08218,PROTEIN NAMES: Outer spore wall protein LDS2 (Lipid droplets in sporulation protein 2) PROTEIN FAMILY: LDS family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the LDS family. FUNCTION: Involved in spore wall assembly. KEYWORDS: Lipid droplet;Membrane;Reference proteome;Sporulation;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Prospore membrane ; Multi-pass membrane protein Lipid droplet Spore wall Note=Localizes to the ascal side of growing prospore membranes in mid-meiosis II and to the spore wall in post-meiotic cells. Localizes to a specific subset of lipid droplets associated with the exterior surface of the spore throughout spore wall formation. MISCELLANEOUS: Present with 155 molecules/cell in log phase SD medium. Q08220,PROTEIN NAMES: Glutathione synthetase GSH2 (GSH synthetase) (GSH-S) (Glutathione synthase) PROTEIN FAMILY: Eukaryotic GSH synthase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the eukaryotic GSH synthase family. PATHWAY: Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2. KEYWORDS: 3D-structure;ATP-binding;Glutathione biosynthesis;Ligase;Magnesium;Metal-binding;Nucleotide-binding;Reference proteome MISCELLANEOUS: Present with 3430 molecules/cell in log phase SD medium. Q08231,"PROTEIN NAMES: Nuclear mRNA export protein THP1 (Bud site selection protein 29) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Component of the SAC3-THP1 complex, which functions in transcription-coupled mRNA export from the nucleus to the cytoplasm. SAC3-THP1 functions in docking export-competent ribonucleoprotein particles (mRNPs) to the nuclear entrance of the nuclear pore complex (nuclear basket), by association with components of the nuclear mRNA export machinery (MEX67-MTR2 and SUB2) in the nucleoplasm and the nucleoporin NUP1 at the nuclear basket. THP1 binds to RNA in vitro. KEYWORDS: 3D-structure;mRNA transport;Nucleus;Reference proteome;Transport SUBCELLULAR LOCATION: Nucleus envelope Note=Localizes to the nuclear pores. MISCELLANEOUS: Present with 1140 molecules/cell in log phase SD medium." Q08285,"PROTEIN NAMES: Exosome complex component RRP40 (Ribosomal RNA-processing protein 40) PROTEIN FAMILY: RRP40 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the RRP40 family. FUNCTION: Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and cryptic unstable transcripts (CUTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and in RNA surveillance pathways, preventing translation of aberrant mRNAs. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. RRP40 as peripheral part of the Exo-9 complex is thought to stabilize the hexameric ring of RNase PH-domain subunits. KEYWORDS: 3D-structure;Cytoplasm;Exosome;Nucleus;Reference proteome;RNA-binding;rRNA processing SUBCELLULAR LOCATION: Cytoplasm Nucleus, nucleolus MISCELLANEOUS: Present with 6050 molecules/cell in log phase SD medium." Q08288,"PROTEIN NAMES: Cell growth-regulating nucleolar protein (Ly1 antibody-reactive protein) (Protein expressed in male leptotene and zygotene spermatocytes 264) (MLZ-264) ORGANISM: Mus musculus (Mouse) FUNCTION: Plays a role in the maintenance of the appropriate processing of 47S/45S pre-rRNA to 32S/30S pre-rRNAs and their subsequent processing to produce 18S and 28S rRNAs (By similarity). Also acts at the level of transcription regulation. Along with PRMT5, binds embryonic globin promoter (By similarity). Represses the expression of embryonic globin Hbb-y gene. In neuroblastoma cells, may also repress the expression of oxidative stress genes, including CHAC1, HMOX1, SLC7A11, ULBP1 and that encoding the small nucleolar RNA SNORD41 (By similarity). Preferentially binds to a DNA motif containing 5'-GGTTAT-3' (By similarity). Negatively regulates the antiviral innate immune response by targeting IRF3 and impairing its DNA-binding activity (By similarity). In addition, inhibits NF-kappa-B-mediated expression of pro-inflammatory cytokines (By similarity). Stimulates phagocytosis of photoreceptor outer segments by retinal pigment epithelial cells. Prevents NCL self-cleavage, maintaining a normal steady-state level of NCL protein in undifferentiated embryonic stem cells (ESCs), which in turn is essential for ESC self-renewal. KEYWORDS: 3D-structure;Cell projection;Coiled coil;Cytoplasm;DNA-binding;Immunity;Innate immunity;Isopeptide bond;Metal-binding;Nucleus;Reference proteome;Repeat;Repressor;Transcription;Transcription regulation;Ubl conjugation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus, nucleolus Cytoplasm Cell projection, cilium, photoreceptor outer segment Note=Component of pre-ribosomal particles, including pre-40S, pre-60S and pre-90S (By similarity). Associated with cytoplasmic ribosomes, but not polysomes, as a component of the 60S subunit. In the retina, predominantly expressed in photoreceptor outer segments. In the nucleolus, colocalizes with nucleolin/NCL, therefore may reside in the dense fibrillar component (DFC). " Q08345,"PROTEIN NAMES: Epithelial discoidin domain-containing receptor 1 (Epithelial discoidin domain receptor 1) (CD167 antigen-like family member A) (Cell adhesion kinase) (Discoidin receptor tyrosine kinase) (HGK2) (Mammary carcinoma kinase 10) (MCK-10) (Protein-tyrosine kinase 3A) (Protein-tyrosine kinase RTK-6) (TRK E) (Tyrosine kinase DDR) (Tyrosine-protein kinase CAK) (CD antigen CD167a) PROTEIN FAMILY: Protein kinase superfamily, Tyr protein kinase family, Insulin receptor subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily. FUNCTION: Tyrosine kinase that functions as a cell surface receptor for fibrillar collagen and regulates cell attachment to the extracellular matrix, remodeling of the extracellular matrix, cell migration, differentiation, survival and cell proliferation. Collagen binding triggers a signaling pathway that involves SRC and leads to the activation of MAP kinases. Regulates remodeling of the extracellular matrix by up-regulation of the matrix metalloproteinases MMP2, MMP7 and MMP9, and thereby facilitates cell migration and wound healing. Required for normal blastocyst implantation during pregnancy, for normal mammary gland differentiation and normal lactation. Required for normal ear morphology and normal hearing (By similarity). Promotes smooth muscle cell migration, and thereby contributes to arterial wound healing. Also plays a role in tumor cell invasion. Phosphorylates PTPN11. KEYWORDS: 3D-structure;Alternative splicing;ATP-binding;Calcium;Cell membrane;Direct protein sequencing;Disulfide bond;Glycoprotein;Kinase;Lactation;Membrane;Metal-binding;Nucleotide-binding;Phosphoprotein;Pregnancy;Receptor;Reference proteome;Secreted;Signal;Transferase;Transmembrane;Transmembrane helix;Tyrosine-protein kinase SUBCELLULAR LOCATION: [Isoform 1]: Cell membrane; Single-pass type I membrane protein.; SUBCELLULAR LOCATION: [Isoform 2]: Cell membrane; Single-pass type I membrane protein.; SUBCELLULAR LOCATION: [Isoform 3]: Secreted.; SUBCELLULAR LOCATION: [Isoform 4]: Cell membrane; Single-pass type I membrane protein. MISCELLANEOUS: [Isoform 3]: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay." Q08380,"PROTEIN NAMES: Galectin-3-binding protein (Basement membrane autoantigen p105) (Lectin galactoside-binding soluble 3-binding protein) (Mac-2-binding protein) (MAC2BP) (Mac-2 BP) (Tumor-associated antigen 90K) ORGANISM: Homo sapiens (Human) FUNCTION: Promotes integrin-mediated cell adhesion. May stimulate host defense against viruses and tumor cells. KEYWORDS: 3D-structure;Cell adhesion;Direct protein sequencing;Disulfide bond;Extracellular matrix;Glycoprotein;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted Secreted, extracellular space, extracellular matrix " Q08438,"PROTEIN NAMES: Phosphopantothenoylcysteine decarboxylase subunit VHS3 (Viable in a HAL3 SIT4 background protein 3) PROTEIN FAMILY: HFCD (homooligomeric flavin containing Cys decarboxylase) superfamily ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the HFCD (homooligomeric flavin containing Cys decarboxylase) superfamily. FUNCTION: Component of the phosphopantothenoylcysteine decarboxylase (PPCDC) involved in the coenzyme A synthesis. Acts as an inhibitory subunit of protein phosphatase PPZ1, which is involved in many cellular processes such as G1-S transition or salt tolerance. KEYWORDS: Cell cycle;Coenzyme A biosynthesis;Phosphoprotein;Protein phosphatase inhibitor;Reference proteome " Q08444,"PROTEIN NAMES: 20S-pre-rRNA D-site endonuclease NOB1 (NIN1-binding protein) (Pre-rRNA-processing endonuclease NOB1) PROTEIN FAMILY: NOB1 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the NOB1 family. FUNCTION: Required for the synthesis of 40S ribosome subunits. Has a role in processing 20S pre-rRNA into the mature 18S rRNA, where it is required for cleavage at the 3' end of the mature 18S rRNA (D-site). Accompanies the 20S pre-rRNA from the nucleus to the cytoplasm. In association with NIN1, may promote the recruitment of the proteasome to the ribosomal subunits stalled in maturation. KEYWORDS: 3D-structure;Cytoplasm;Endoplasmic reticulum;Hydrolase;Metal-binding;Nuclease;Nucleus;Reference proteome;Ribosome biogenesis;Zinc SUBCELLULAR LOCATION: Cytoplasm. Nucleus, nucleolus. Endoplasmic reticulum. MISCELLANEOUS: Present with 4490 molecules/cell in log phase SD medium." Q08558,"PROTEIN NAMES: Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase (Peroxisomal di-isomerase DCI1) PROTEIN FAMILY: Enoyl-CoA hydratase/isomerase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the enoyl-CoA hydratase/isomerase family. FUNCTION: Peroxisomal di-isomerase that is involved in fatty acid metabolism enzyme by converting 3,5-dienoyl-CoAs to the corresponding 2,4-dienoyl-CoAs. Required for ECI1 to be locazed to the peroxisome. PATHWAY: Lipid metabolism; fatty acid beta-oxidation. KEYWORDS: Fatty acid metabolism;Isomerase;Lipid metabolism;Peroxisome;Reference proteome SUBCELLULAR LOCATION: Peroxisome " Q08641,"PROTEIN NAMES: tRNA(Thr) (cytosine(32)-N(3))-methyltransferase (Actin-binding protein of 140 kDa) (tRNA methyltransferase of 140 kDa) PROTEIN FAMILY: Methyltransferase superfamily, METL family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the methyltransferase superfamily. METL family. FUNCTION: S-adenosyl-L-methionine-dependent methyltransferase that mediates N(3)-methylcytidine modification of residue 32 of the tRNA anticodon loop of tRNA(Thr) and tRNA(Ser). N(3)-methylcytidine methylation of tRNA(Thr) requires the N6-threonylcarbamoylation of tRNA (t6A37) by the EKC/KEOPS complex as prerequisite. N(3)-methylcytidine methylation of tRNA(Ser) requires the formation of N(6)-dimethylallyladenosine(37) (i6A37) by MOD5 as prerequisite. Methylation of tRNA(Ser) is also stimulated by SES1. Binds F-actin and shows weak F-actin cross-linking activity. KEYWORDS: 3D-structure;Actin-binding;Cytoplasm;Cytoskeleton;Direct protein sequencing;Methyltransferase;Phosphoprotein;Reference proteome;Ribosomal frameshifting;S-adenosyl-L-methionine;Transferase;tRNA processing SUBCELLULAR LOCATION: Cytoplasm Cytoplasm, cytoskeleton Note=Cytoplasmic and cortical cytoskeleton. MISCELLANEOUS: N- and C-terminal domains are encoded in separate ORFs that are translated into one protein via a +1 frameshift. ABP140 mRNA translation follows a cotranslational transport: mRNA is transported to the distal pole of the mother cell, independently of the SHE machinery, and follows a translational coupling, in which ABP140 mRNA is tethered to actin cables via its nascent protein product and is transported to the distal pole by actin retrograde flow.; MISCELLANEOUS: [Isoform 1]: Produced by ribosomal frameshifting between codon Leu-277 and Gly-278." Q08702,"PROTEIN NAMES: Aprataxin-like protein (Hit family protein 3) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: DNA-binding protein involved in single-strand DNA break repair, double-strand DNA break repair and base excision repair. Resolves abortive DNA ligation intermediates formed either at base excision sites, or when DNA ligases attempt to repair non-ligatable breaks induced by reactive oxygen species. Catalyzes the release of adenylate groups covalently linked to 5'-phosphate termini, resulting in the production of 5'-phosphate termini that can be efficiently rejoined. Likewise, catalyzes the release of 3'-linked guanosine (DNAppG) and inosine (DNAppI) from DNA, but has higher specific activity with 5'-linked adenosine (AppDNA) (By similarity). KEYWORDS: Cytoplasm;DNA damage;DNA repair;DNA-binding;Hydrolase;Metal-binding;Nucleus;Reference proteome;Zinc SUBCELLULAR LOCATION: Nucleus Cytoplasm MISCELLANEOUS: Present with 396 molecules/cell in log phase SD medium." Q08760,"PROTEIN NAMES: Bud site selection protein RAX1 (Revert to axial protein 1) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Required for the establishment of the bipolar budding pattern. Involved in selecting bud sites at both the distal and proximal poles of daughter cells as well as near previously used division sites on mother cells. Has a role in the localization of BUD8, the distal bipolar budding landmark, and of BUD9, the proximal pole landmark. KEYWORDS: Cell cycle;Cell division;Cell membrane;Membrane;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Bud neck. Bud tip. Note=Before cytokinesis, RAX1 concentrates as a ring at the mother-bud neck and to the tip of the bud. The RAX1 ring splits at cytokinesis, endowing each progeny cell with a RAX1 ring and additional RAX1 localization at the distal bud pole of the newborn daughter cell. The rings persist at the cell cortex for several generations, giving rise to cells decorated by multiple rings. " Q08775,"PROTEIN NAMES: Runt-related transcription factor 2 (Acute myeloid leukemia 3 protein) (Core-binding factor subunit alpha-1) (CBF-alpha-1) (Oncogene AML-3) (Osteoblast-specific transcription factor 2) (OSF-2) (Polyomavirus enhancer-binding protein 2 alpha A subunit) (PEA2-alpha A) (PEBP2-alpha A) (SL3-3 enhancer factor 1 alpha A subunit) (SL3/AKV core-binding factor alpha A subunit) ORGANISM: Mus musculus (Mouse) FUNCTION: Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters. Inhibits KAT6B-dependent transcriptional activation (By similarity). In osteoblasts, supports transcription activation: synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE). KEYWORDS: Alternative splicing;Cytoplasm;Differentiation;Direct protein sequencing;DNA-binding;Isopeptide bond;Methylation;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation;Ubl conjugation SUBCELLULAR LOCATION: Nucleus Cytoplasm " Q08886,"PROTEIN NAMES: Guanine nucleotide-binding protein subunit beta 1 (Gbeta mimic kelch protein 1) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Beta subunit of a guanine nucleotide-binding protein (G proteins). G proteins are involved as modulators or transducers in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction. Involved in the determination of the cAMP level according to nutritional conditions, most probably as a regulator of cAMP phosphodiesterase. Required for the control of pseudohyphal and haploid invasive growth. KEYWORDS: Cytoplasm;Kelch repeat;Phosphoprotein;Reference proteome;Repeat SUBCELLULAR LOCATION: Cytoplasm MISCELLANEOUS: Present with 1640 molecules/cell in log phase SD medium." Q08890,PROTEIN NAMES: Iduronate 2-sulfatase (Alpha-L-iduronate sulfate sulfatase) PROTEIN FAMILY: Sulfatase family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the sulfatase family. FUNCTION: Lysosomal enzyme involved in the degradation pathway of dermatan sulfate and heparan sulfate. KEYWORDS: Calcium;Disulfide bond;Glycoprotein;Hydrolase;Lysosome;Metal-binding;Reference proteome;Signal;Zymogen SUBCELLULAR LOCATION: Lysosome Q08923,"PROTEIN NAMES: Histone deacetylase complex subunit CTI6 (CYC8-TUP1-interacting protein 6) (Transcriptional regulatory protein CTI6) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Component of the RPD3C(L) histone deacetylase complex (HDAC). Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. CTI6 links the SAGA coactivator to the CYC8-TUP1 corepressor. Involved in transcription regulation of heme-regulated genes and required for GCN5 recruitment, histone H3 acetylation and SPT15/TBP binding to promoters. KEYWORDS: Chromatin regulator;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Repressor;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: Present with 1590 molecules/cell in log phase SD medium." Q08949,PROTEIN NAMES: DNA damage checkpoint protein 1 PROTEIN FAMILY: DDC1 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the DDC1 family. FUNCTION: Component of the checkpoint clamp complex involved in the surveillance mechanism that allows the DNA repair pathways to act to restore the integrity of the DNA prior to DNA synthesis or separation of the replicated chromosomes. Associates with sites of DNA damage and modulates the MEC1 signaling pathway and the activation of RAD53 in response to DNA damage at phase G1. The complex also physically regulates DNA polymerase zeta-dependent mutagenesis by controlling the access of polymerase zeta to damaged DNA. KEYWORDS: 3D-structure;Cytoplasm;DNA damage;DNA repair;DNA-binding;Nucleus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cytoplasm. Nucleus. MISCELLANEOUS: Present with 238 molecules/cell in log phase SD medium. Q08985,PROTEIN NAMES: Homocysteine S-methyltransferase 2 (S-adenosylmethionine metabolism protein 4) (S-methylmethionine:homocysteine methyltransferase 2) (SMM:Hcy S-methyltransferase 2) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Homocysteine S-methyltransferase involved in the conversion of S-adenosylmethionine (AdoMet) to methionine to control the methionine/AdoMet ratio. Converts also S-methylmethionine (SMM) to methionine. KEYWORDS: Amino-acid biosynthesis;Cytoplasm;Metal-binding;Methionine biosynthesis;Methyltransferase;Nucleus;Phosphoprotein;Reference proteome;S-adenosyl-L-methionine;Transferase;Zinc SUBCELLULAR LOCATION: Cytoplasm Nucleus MISCELLANEOUS: Present with 60300 molecules/cell in log phase SD medium. Q08986,"PROTEIN NAMES: S-adenosylmethionine permease SAM3 (S-adenosylmethionine metabolism protein 3) PROTEIN FAMILY: Amino acid-polyamine-organocation (APC) superfamily, YAT family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the amino acid-polyamine-organocation (APC) superfamily. YAT (TC 2.A.3.10) family. FUNCTION: High-affinity S-adenosylmethionine permease, required for utilization of S-adenosylmethionine as a sulfur source. KEYWORDS: Amino-acid transport;Endoplasmic reticulum;Glycoprotein;Membrane;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Membrane ; Multi-pass membrane protein Endoplasmic reticulum MISCELLANEOUS: Present with 1300 molecules/cell in log phase SD medium." Q08BB2,"PROTEIN NAMES: N-fatty-acyl-amino acid synthase/hydrolase PM20D1.2 (Peptidase M20 domain-containing protein 1.2) PROTEIN FAMILY: Peptidase M20A family ORGANISM: Danio rerio (Zebrafish) (Brachydanio rerio) SIMILARITY: Belongs to the peptidase M20A family. FUNCTION: Secreted enzyme that regulates the endogenous N-fatty acyl amino acid (NAAs) tissue and circulating levels by functioning as a bidirectional NAA synthase/hydrolase. It condenses free fatty acids and free amino acids to generate NAAs and bidirectionally catalyzes the reverse hydrolysis reaction. Some of these NAAs stimulate oxidative metabolism via mitochondrial uncoupling, increasing energy expenditure in a UPC1-independent manner. Thereby, this secreted protein may indirectly regulate whole body energy expenditure. PM20D1 circulates in tight association with both low- and high-density (LDL and HDL,respectively) lipoprotein particles. PATHWAY: Amino-acid metabolism.; PATHWAY: Energy metabolism; electron transfer.; PATHWAY: Lipid metabolism; fatty acid metabolism. KEYWORDS: Glycoprotein;Hydrolase;Lyase;Metal-binding;Protease;Reference proteome;Secreted;Signal;Zinc SUBCELLULAR LOCATION: Secreted " Q08DW9,"PROTEIN NAMES: Lysophosphatidylserine lipase ABHD12 (2-arachidonoylglycerol hydrolase ABHD12) (Abhydrolase domain-containing protein 12) (Monoacylglycerol lipase ABHD12) (Oxidized phosphatidylserine lipase ABHD12) PROTEIN FAMILY: Serine esterase family ORGANISM: Bos taurus (Bovine) SIMILARITY: Belongs to the serine esterase family. FUNCTION: Lysophosphatidylserine (LPS) lipase that mediates the hydrolysis of lysophosphatidylserine, a class of signaling lipids that regulates immunological and neurological processes (By similarity). Represents a major lysophosphatidylserine lipase in the brain, thereby playing a key role in the central nervous system (By similarity). Also able to hydrolyze oxidized phosphatidylserine; oxidized phosphatidylserine is produced in response to severe inflammatory stress and constitutes a proapoptotic 'eat me' signal. Also has monoacylglycerol (MAG) lipase activity: hydrolyzes 2-arachidonoylglycerol (2-AG), thereby acting as a regulator of endocannabinoid signaling pathways. Has a strong preference for very-long-chain lipid substrates; substrate specificity is likely due to improved catalysis and not improved substrate binding (By similarity). KEYWORDS: Endoplasmic reticulum;Glycoprotein;Hydrolase;Lipid metabolism;Membrane;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Single-pass membrane protein " Q09024,"PROTEIN NAMES: Neural/ectodermal development factor IMP-L2 ORGANISM: Drosophila melanogaster (Fruit fly) FUNCTION: Essential developmental role during embryogenesis, in particular the normal development of the nervous system. May be involved in some aspect of cell adhesion. KEYWORDS: 3D-structure;Alternative splicing;Cell adhesion;Developmental protein;Disulfide bond;Immunoglobulin domain;Reference proteome;Repeat;Secreted;Signal SUBCELLULAR LOCATION: Secreted, extracellular space. " Q09092,"PROTEIN NAMES: Putative serine/threonine-protein kinase receptor (S-receptor kinase) (SRK) PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family ORGANISM: Brassica oleracea var. viridis (Flowering kale) (Brassica oleracea var. acephala) SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. FUNCTION: Involved in sporophytic self-incompatibility system (the inability of flowering plants to achieve self-fertilization), probably acting in combination with S-locus-specific glycoproteins. Interaction with a ligand in the extracellular domain triggers the protein kinase activity of the cytoplasmic domain. KEYWORDS: ATP-binding;Disulfide bond;Glycoprotein;Kinase;Membrane;Nucleotide-binding;Receptor;Self-incompatibility;Serine/threonine-protein kinase;Signal;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. " Q09098,PROTEIN NAMES: Prostate and testis expressed protein 4 (Calcium transport inhibitor) (Caltrin) (PATE-like protein B) (PATE-B) (Seminal vesicle protein 7) (Seminal vesicle protein VII) (SVS VII) PROTEIN FAMILY: PATE family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the PATE family. FUNCTION: Enhances sperm motility. Binds to calmodulin and inhibits calcium transport into spermatozoa. May modulate the function of nicotinic acetylcholine receptors. KEYWORDS: Calcium;Calmodulin-binding;Direct protein sequencing;Disulfide bond;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted. Q09101,PROTEIN NAMES: Locomotion-related protein Hikaru genki ORGANISM: Drosophila melanogaster (Fruit fly) FUNCTION: Plays a role in the formation of functional neural circuits from the early stages of synapse formation. Has a role in the development of CNS functions involved in locomotor activity. KEYWORDS: Alternative splicing;Developmental protein;Differentiation;Disulfide bond;Glycoprotein;Immunoglobulin domain;Neurogenesis;Reference proteome;Repeat;Secreted;Signal;Sushi SUBCELLULAR LOCATION: Secreted Q09170,"PROTEIN NAMES: Serine/threonine-protein kinase cds1 (Checkpoint kinase cds1) PROTEIN FAMILY: Protein kinase superfamily, CAMK Ser/Thr protein kinase family, CHEK2 subfamily ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CHEK2 subfamily. FUNCTION: Has a role in the DNA replication-monitoring S/G2 checkpoint system. It is responsible for blocking mitosis in the S phase. It monitors DNA synthesis by interacting with DNA polymerase alpha and sends a signal to block the onset of mitosis while DNA synthesis is in progress. Phosphorylates rad60 and dna2. KEYWORDS: ATP-binding;Cell cycle;Kinase;Nucleotide-binding;Phosphoprotein;Reference proteome;Serine/threonine-protein kinase;Transferase " Q09175,"PROTEIN NAMES: Dibasic-processing endoprotease (KEX2-related protease) PROTEIN FAMILY: Peptidase S8 family, Furin subfamily ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the peptidase S8 family. Furin subfamily. FUNCTION: Membrane-bound, subtilisin-like serine protease that processes the P-factor precursor and other precursor proteins. Essential for cell viability. Cleaves substrate on the C-terminal side of dibasic residues. KEYWORDS: Calcium;Cleavage on pair of basic residues;Disulfide bond;Glycoprotein;Golgi apparatus;Hydrolase;Membrane;Protease;Reference proteome;Serine protease;Signal;Transmembrane;Transmembrane helix;Zymogen SUBCELLULAR LOCATION: Golgi apparatus, trans-Golgi network membrane ; Single-pass type I membrane protein " Q09251,"PROTEIN NAMES: E3 ubiquitin ligase rnf-121 (RING finger protein 121) PROTEIN FAMILY: RNF121 family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the RNF121 family. FUNCTION: E3 ubiquitin ligase which accepts ubiquitin and transfers it to substrates such as the beta-integrin subunit pat-3, promoting their degradation by the endoplasmic reticulum-associated degradation (ERAD) pathway which is a pathway involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins. Negatively regulates the unfolded protein response to reduce endoplasmic reticulum stress. Required for the cessation of distal tip cell migration at the end of larval morphogenesis. Plays a role in germline and gonad development. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Endoplasmic reticulum;Golgi apparatus;Membrane;Metal-binding;Reference proteome;Transferase;Transmembrane;Transmembrane helix;Zinc;Zinc-finger SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein Golgi apparatus membrane ; Multi-pass membrane protein " Q09274,PROTEIN NAMES: Degenerin-like protein unc-105 (Uncoordinated protein 105) PROTEIN FAMILY: Amiloride-sensitive sodium channel family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family. FUNCTION: Ion channel which is permeable to small monovalent cations. Shown not to be H+-ion gated. May be mechanosensitive and is required for growth and muscle development. KEYWORDS: Alternative splicing;Developmental protein;Differentiation;Glycoprotein;Ion channel;Ion transport;Membrane;Myogenesis;Reference proteome;Sodium;Sodium channel;Sodium transport;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. Q09276,"PROTEIN NAMES: Protein dyf-7 (Abnormal dye filling protein 7) ORGANISM: Caenorhabditis elegans FUNCTION: Required for permeability of amphid and phasmid neurons to external dyes, chemotaxis to ammonium chloride, avoidance of high osmotic stimuli, male mating and dauer formation. Along with dex-1, enables neurite growth and maintenance by anchoring amphid dendritic tips during neuron cell body migration in embryonic and larval development. KEYWORDS: Cell membrane;Cell projection;Disulfide bond;Membrane;Neurogenesis;Reference proteome;Secreted;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass membrane protein Cell projection, dendrite Secreted Note=Located at dendritic tips. Secreted following proteolytic cleavage in vitro. " Q09293,"PROTEIN NAMES: Embryonic developmental protein tofu-6 (21U-RNA biogenesis fouled up protein 6) (Maternal effect lethal protein 47) ORGANISM: Caenorhabditis elegans FUNCTION: Component of the pid-1 and tost-1 variants of the PETISCO complexes, which have roles in the biogenesis of a class of 21 nucleotide PIWI-interacting RNAs (piRNAs) that possess a uracil residue at the 5'-end (also called 21U-RNAs) and embryogenesis, respectively. Promotes the biogenesis of 21U-RNAs. Mediates the interaction between the PETISCO complex and the PUCH complex, the endoribonuclease complex processing the 5'-end of precursor piRNAs, thereby enhancing mature piRNA production. Required for chromosome segregation and cell division in early embryos. May have a role in DNA replication. KEYWORDS: 3D-structure;Cell cycle;Cell division;Chromosome partition;Cytoplasm;Developmental protein;Nucleus;Reference proteome;RNA-binding;RNA-mediated gene silencing SUBCELLULAR LOCATION: Cytoplasm Cytoplasm, perinuclear region Nucleus Note=Dispersedly distributes throughout the cytoplasm in early embryos. During early embryogenesis, localizes to the nucleus at prophase of cell division, and remains in the cytosol at interphase in 2- and 4-cell embryos. Does not localize to cytoplasmic granules in oocytes and embryos. Localizes to puncta in the perinuclear region in the germline syncytium. Localization to the perinuclear region in the germline is dependent on pid-1, tost-1, pics-1 and erh-2. " Q09306,"PROTEIN NAMES: Tubby protein homolog 1 PROTEIN FAMILY: TUB family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the TUB family. FUNCTION: Has a role in fat regulation independent of daf-16. Implicated in ciliar sensory function which is required for normal sensory behavior such as chemotaxis. Required for extension and growth of sensory neuronal cilia during postembryonic development, potentially via mediating signaling protein transport and localization of PI(4,5)P2 to the ciliary base. Functions in life span control via the insulin/IGF-1 pathway. Thought to be involved in neuronal trafficking. KEYWORDS: Cell projection;Chemotaxis;Cytoplasm;Lipid metabolism;Reference proteome;Sensory transduction SUBCELLULAR LOCATION: Cytoplasm Cell projection, axon Cell projection, dendrite Cell projection, cilium Note=Expressed in the periciliary membrane compartment in AWB and ASK sensory neurons. " Q09314,"PROTEIN NAMES: Pseudocleavage protein nop-1 ORGANISM: Caenorhabditis elegans FUNCTION: Required for formation of the pseudocleavage furrow during the first cleavage of the embryo and also mediates aster-induced furrowing during cytokinesis. Promotes cortical recruitment of ani-1 and nmy-2 during pseudocleavage and cytokinesis and promotes the accumulation of actin at furrowing regions. Regulates establishment of embryonic cell polarity. KEYWORDS: Alternative splicing;Cell cycle;Cell division;Cytoplasm;Developmental protein;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus Cytoplasm Cytoplasm, cell cortex Cleavage furrow Note=Highly concentrated in interphase nuclei. As well as a significant cytoplasmic pool, a distinct cortical localization is also detected, particularly at ingressing pseudocleavage and cleavage furrows. " Q09328,"PROTEIN NAMES: Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A (Alpha-mannoside beta-1,6-N-acetylglucosaminyltransferase V) (GlcNAc-T V) (GNT-V) (Mannoside acetylglucosaminyltransferase 5) (N-acetylglucosaminyl-transferase V) [Cleaved into: Secreted alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A (Secreted beta-1,6-N-acetylglucosaminyltransferase V) (Secreted GNT-V)] PROTEIN FAMILY: Glycosyltransferase 18 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the glycosyltransferase 18 family. FUNCTION: Catalyzes the addition of N-acetylglucosamine (GlcNAc) in beta 1-6 linkage to the alpha-linked mannose of biantennary N-linked oligosaccharides. Catalyzes an important step in the biosynthesis of branched, complex-type N-glycans, such as those found on EGFR, TGFR (TGF-beta receptor) and CDH2. Via its role in the biosynthesis of complex N-glycans, plays an important role in the activation of cellular signaling pathways, reorganization of the actin cytoskeleton, cell-cell adhesion and cell migration. MGAT5-dependent EGFR N-glycosylation enhances the interaction between EGFR and LGALS3 and thereby prevents rapid EGFR endocytosis and prolongs EGFR signaling. Required for efficient interaction between TGFB1 and its receptor. Enhances activation of intracellular signaling pathways by several types of growth factors, including FGF2, PDGF, IGF, TGFB1 and EGF. MGAT5-dependent CDH2 N-glycosylation inhibits CDH2-mediated homotypic cell-cell adhesion and contributes to the regulation of downstream signaling pathways. Promotes cell migration. Contributes to the regulation of the inflammatory response. MGAT5-dependent TCR N-glycosylation enhances the interaction between TCR and LGALS3, limits agonist-induced TCR clustering, and thereby dampens TCR-mediated responses to antigens. Required for normal leukocyte evasation and accumulation at sites of inflammation (By similarity). Inhibits attachment of monocytes to the vascular endothelium and subsequent monocyte diapedesis.; FUNCTION: [Secreted alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A]: Promotes proliferation of umbilical vein endothelial cells and angiogenesis, at least in part by promoting the release of the growth factor FGF2 from the extracellular matrix. PATHWAY: Protein modification; protein glycosylation. KEYWORDS: 3D-structure;Direct protein sequencing;Disulfide bond;Glycoprotein;Glycosyltransferase;Golgi apparatus;Membrane;Reference proteome;Secreted;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus membrane ; Single-pass type II membrane protein.; SUBCELLULAR LOCATION: [Secreted alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A]: Secreted " Q09330,PROTEIN NAMES: mRNA export protein mlo3 (RNA-annealing protein mlo3) ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) FUNCTION: Has a role in the mRNA export process. Interferes with mitotic chromosome segregation when overexpressed. KEYWORDS: mRNA transport;Nucleus;Reference proteome;RNA-binding;Transport SUBCELLULAR LOCATION: Nucleus Q09349,"PROTEIN NAMES: Ubiquitin conjugation factor E4 ufd-2 (E4 ubiquitin-protein ligase ufd-2) (RING-type E3 ubiquitin transferase E4) (Ubiquitin fusion degradation protein 2) PROTEIN FAMILY: Ubiquitin conjugation factor E4 family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the ubiquitin conjugation factor E4 family. FUNCTION: Acts as an E4 ubiquitin ligase mediating the assembly of polyubiquitin chains on substrates ubiquitinated by another E3 ubiquitin ligase. The elongation of preexisting ubiquitin chains preferentially targets ubiquitin 'Lys-29' and 'Lys-48' residues. Also functions as an E3 ligase in conjunction with specific E1 and E2 ligases. Probably by regulating protein ubiquitination at DNA damage repair sites, coordinates DNA double-strand-break repair and apoptosis in the germline. Required for germline apoptosis in response to DNA damage downstream of cep-1. Involved in the resolution of DNA-repair sites by promoting the release of rad-51 from DNA damage foci. In association with protein-ligase chn-1, acts as an E3/E4 ligase to poly-ubiquitinate lysine residues in the UCS domain of myosin chaperone unc-45. By targeting myosin chaperone unc-45 for proteasomal degradation, regulates myosin assembly in body wall muscles in association with cdc-48.1 and chn-1. However, in a contrasting study, acts as an E3 ligase, independently of chn-1, to poly-ubiquitinate unc-45 without promoting unc-45 proteasomal degradation. Instead, uses unc-45 as an adapter protein to recruit and poly-ubiquitinate unfolded myosin heavy chain B unc-54. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Alternative splicing;Cytoplasm;Membrane;Nucleus;Reference proteome;Transferase;Ubl conjugation pathway SUBCELLULAR LOCATION: Cytoplasm Nucleus membrane ; Peripheral membrane protein ; Cytoplasmic side Nucleus, nucleolus Note=Localizes to germline syncytium. In the late pachytene, accumulates at the nuclear periphery forming a ring. Following ionizing radiation-mediated DNA damage, localizes to foci within nucleoli where it colocalizes with cdc-48.1 and/or cdc-48.2, atx-3, proteasome alpha subunit and ubiquitinated proteins. Localization to foci is ubiquitin-dependent and regulated by E3 ligase hecd-1 and deubiquitinating enzyme atx-3. ufd-2 foci are formed following the initiation of homologous recombination (HR) and persist until HR is completed. ufd-2 foci are also formed upon cep-1 activation. " Q09353,PROTEIN NAMES: Sentrin-specific protease (SUMO protease) (SuPr) (Ubiquitin-like protease) PROTEIN FAMILY: Peptidase C48 family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the peptidase C48 family. FUNCTION: Protease that deconjugates smo-1 from targeted proteins and may catalyze the processing of smo-1 to its mature form. KEYWORDS: Hydrolase;Nucleus;Protease;Reference proteome;Thiol protease SUBCELLULAR LOCATION: Nucleus envelope Q09373,"PROTEIN NAMES: WD repeat-containing protein fzy-1 (CDC20 protein family homolog 1) (Fizzy protein 1) PROTEIN FAMILY: WD repeat CDC20/Fizzy family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the WD repeat CDC20/Fizzy family. FUNCTION: Plays a role in metaphase-anaphase transition during meiosis I. Required for embryonic anterior-posterior axis formation. KEYWORDS: Cell cycle;Cell division;Chromosome;Cytoplasm;Meiosis;Mitosis;Reference proteome;Repeat;WD repeat SUBCELLULAR LOCATION: Chromosome Cytoplasm Note=At prometaphase, localizes around condensed chromosomes. At metaphase, localizes along chromosomes, dissociates from the sister chromatid after separation and localizes to the cytoplasm at anaphase and interphase. " Q09374,"PROTEIN NAMES: Deoxynucleoside triphosphate triphosphohydrolase sahd-1 (dNTPase) (SAM domain and HD domain-containing protein 1) (SAMHD1 homolog) PROTEIN FAMILY: SAMHD1 family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the SAMHD1 family. FUNCTION: Has deoxynucleoside triphosphate (dNTPase) activity. dNTPase activity acts as a regulator of DNA precursor pools by regulating dNTP pools (By similarity). Phosphorylation acts as a switch to control dNTPase-dependent and -independent functions (By similarity). KEYWORDS: 3D-structure;Allosteric enzyme;Alternative splicing;Chromosome;GTP-binding;Hydrolase;Metal-binding;Nucleotide-binding;Nucleus;Phosphoprotein;Reference proteome;Zinc SUBCELLULAR LOCATION: Nucleus Chromosome MISCELLANEOUS: Does not hydrolyze dGMP and dGDP. Shows significantly reduced capability to hydrolyze the chain terminator triphosphates ddGTP, ddITP, ddTTP or 3'-azido-3'-deoxythymidine (AZT), in the presence of dGTP activator. Able to hydrolyze an anti-cancer agent, araCTP." Q09426,"PROTEIN NAMES: 2-hydroxyacylsphingosine 1-beta-galactosyltransferase (Ceramide UDP-galactosyltransferase) (CGalT) (Cerebroside synthase) (UDP-galactose-ceramide galactosyltransferase) PROTEIN FAMILY: UDP-glycosyltransferase family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the UDP-glycosyltransferase family. FUNCTION: Catalyzes the transfer of galactose to ceramide, a key enzymatic step in the biosynthesis of galactocerebrosides, which are abundant sphingolipids of the myelin membrane of the central nervous system and peripheral nervous system. Galactosylates both hydroxy- and non-hydroxy fatty acid-containing ceramides and diglycerides. PATHWAY: Sphingolipid metabolism; galactosylceramide biosynthesis. KEYWORDS: Endoplasmic reticulum;Glycoprotein;Glycosyltransferase;Lipid metabolism;Membrane;Reference proteome;Signal;Sphingolipid metabolism;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Single-pass membrane protein Endoplasmic reticulum " Q09476,"PROTEIN NAMES: Paxillin homolog 1 PROTEIN FAMILY: Paxillin family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the paxillin family. FUNCTION: Required for myofilament organization of the pharyngeal sarcomeres and for pharyngeal muscle contractions and hence for pharyngeal pumping. Together with lin-8, might be required for myofilament organization in the body wall muscles. KEYWORDS: Alternative splicing;Cell junction;Cell membrane;Cell projection;Cytoplasm;LIM domain;Membrane;Metal-binding;Reference proteome;Repeat;Zinc SUBCELLULAR LOCATION: [Isoform a]: Cell junction, adherens junction Cell membrane Cytoplasm, myofibril, sarcomere, M line Cell projection, podosome Note=Colocalizes with pat-3 to dense bodies, adhesion plaques and M lines in body wall muscles. Colocalizes with deb-1 in podosome-like structures in the pharyngeal muscle. Requires unc-95 for the localization to dense bodies.; SUBCELLULAR LOCATION: [Isoform c]: Cell projection, podosome " Q09477,"PROTEIN NAMES: Zinc finger protein dpff-1 PROTEIN FAMILY: Requiem/DPF family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the requiem/DPF family. FUNCTION: Probable transcription factor, involved in meiosis and stress protection. KEYWORDS: Alternative splicing;Cytoplasm;Metal-binding;Nucleus;Reference proteome;Repeat;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Cytoplasm Note=Expressed at higher level in nuclei. " Q09500,"PROTEIN NAMES: Cystinosin homolog PROTEIN FAMILY: Cystinosin family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the cystinosin family. FUNCTION: Cystine/H(+) symporter that mediates export of cystine, the oxidized dimer of cysteine, from lysosomes (By similarity). May play a role in the degradation of engulfed apoptotic cells. KEYWORDS: Alternative splicing;Cytoplasmic vesicle;Glycoprotein;Lysosome;Membrane;Reference proteome;Repeat;Symport;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Lysosome membrane ; Multi-pass membrane protein Cytoplasmic vesicle, phagosome Note=During degradation of apoptotic cells when lysosomes fuse to phagosomes, located to phagosomal surfaces until the cell corpse is fully degraded. " Q09555,"PROTEIN NAMES: RFX-like transcription factor daf-19 (Abnormal dauer formation protein 19) PROTEIN FAMILY: RFX family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the RFX family. FUNCTION: Probable transcription factor. May regulate some genes of ciliated sensory neurons. May activate the expression of the shared components of sensory cilia, but not the cell-type-specific expression. Together with transcription factor atf-7, involved in regulation of the serotonergic response of ADF neurons to pathogenic food.; FUNCTION: [Isoform c]: Involved in male mating behavior; may play a role in functional specialization of PKD ciliated sensory neurons. KEYWORDS: Activator;Alternative splicing;DNA-binding;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus.; SUBCELLULAR LOCATION: [Isoform c]: Nucleus " Q09685,PROTEIN NAMES: Pre-mRNA-splicing factor dre4 (DNA replication protein 4) (Hyphal growth protein 1) ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) FUNCTION: Component of the spliceosome involved in mRNA processing. KEYWORDS: mRNA processing;mRNA splicing;Nucleus;Reference proteome;Repeat;Spliceosome SUBCELLULAR LOCATION: Nucleus Q09702,"PROTEIN NAMES: Negative regulator of differentiation 1 (Multicopy suppressor of sporulation protein msa2) ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) FUNCTION: Negative regulator of sexual differentiation. Acts by repressing the transcription of meiosis-inducing, ste11-regulated genes until cells reach a critical level of starvation. RNA-binding protein that preferentially binds poly(U). KEYWORDS: 3D-structure;Phosphoprotein;Reference proteome;Repeat;Repressor;RNA-binding;Transcription;Transcription regulation " Q09751,"PROTEIN NAMES: Lactoylglutathione lyase (Aldoketomutase) (Glyoxalase I) (Glx I) (Ketone-aldehyde mutase) (Methylglyoxalase) (S-D-lactoylglutathione methylglyoxal lyase) PROTEIN FAMILY: Glyoxalase I family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the glyoxalase I family. FUNCTION: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione. PATHWAY: Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 1/2. KEYWORDS: Cobalt;Lyase;Manganese;Metal-binding;Nickel;Reference proteome;Repeat;Zinc " Q09823,PROTEIN NAMES: Linear element protein rec10 (Meiotic recombination protein rec10) ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) FUNCTION: Organizes linear element components on chromosomes and is thus required for meiotic DNA recombination. KEYWORDS: Chromosome;Meiosis;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus Chromosome Note=Localizes to DNA double-strand break (DSB) hotspots. Q09825,"PROTEIN NAMES: Spindle pole body-associated protein sad1 ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) FUNCTION: Associates with the spindle pole body and maintains a functional interface between the nuclear membrane and the microtubule motor proteins. Involved in chromosome segregation during meiosis where it associates with the telomeres. KEYWORDS: 3D-structure;Cell cycle;Cell division;Cytoplasm;Cytoskeleton;Membrane;Microtubule;Mitosis;Nucleus;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body. Nucleus membrane; Single-pass membrane protein. " Q09849,"PROTEIN NAMES: SWI/SNF and RSC complexes subunit arp42 (Actin-related protein 42) (Chromatin structure-remodeling complex subunit arp42) PROTEIN FAMILY: Actin family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the actin family. FUNCTION: Component of the chromatin structure remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. Controls particularly membrane and organelle development genes. Part of the SWI/SNF complex, an ATP-dependent chromatin remodeling complex, required for the positive and negative regulation of gene expression of a large number of genes. It changes chromatin structure by altering DNA-histone contacts within a nucleosome, leading eventually to a change in nucleosome position, thus facilitating or repressing binding of gene-specific transcription factors. KEYWORDS: Chromatin regulator;Cytoplasm;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Cytoplasm Nucleus " Q09851,"PROTEIN NAMES: NADPH-dependent 1-acyldihydroxyacetone phosphate reductase (ADR) (1-acyl DHAP reductase) (Acyl/alkyl DHAP reductase) (Acylglycerone-phosphate reductase) (Triacylglycerol lipase ayr1) (TAG lipase) PROTEIN FAMILY: Short-chain dehydrogenases/reductases (SDR) family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FUNCTION: Can convert acyl and alkyl dihydroxyacetone-phosphate (DHAP) into glycerolipids and ether lipids, respectively. Required for the biosynthesis of phosphatidic acid via the DHAP pathway, where it reduces 1-acyl DHAP to lysophosphatidic acid (LPA). Also has triacylglycerol (TAG) lipase activity. Involved in the mobilization of the non-polar storage lipids triacylglycerols (TAGs) from lipid particles by hydrolysis of TAGs. Lipolysis of TAG by AYR1 is essential for starvation-induced autophagy. Forms an NADPH-regulated cation-selective channel in the mitochondrial outer membrane. KEYWORDS: Cytoplasm;Endoplasmic reticulum;Golgi apparatus;Hydrolase;Lipid degradation;Lipid droplet;Lipid metabolism;Membrane;Mitochondrion;Mitochondrion outer membrane;NADP;Oxidoreductase;Reference proteome;Vacuole SUBCELLULAR LOCATION: Lipid droplet Cytoplasm Vacuole Endoplasmic reticulum Golgi apparatus Mitochondrion outer membrane " Q09898,"PROTEIN NAMES: Serine/threonine-protein kinase sid2 PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. FUNCTION: Part of a signaling pathway. Required for initiation of medial ring constriction and septation. KEYWORDS: ATP-binding;Cell cycle;Cell division;Cytoplasm;Cytoskeleton;Kinase;Mitosis;Nucleotide-binding;Phosphoprotein;Reference proteome;Septation;Serine/threonine-protein kinase;Transferase SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body. Cytoplasm. " Q09906,PROTEIN NAMES: Vacuole membrane protein hfl11 PROTEIN FAMILY: TMEM184 family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the TMEM184 family. FUNCTION: Vacuole membrane protein that recruits ATG8 to facilitate the degradation of vacuolar integral membrane proteins during early-stationary vacuole turnover (EVT) when cells enter stationary phase. KEYWORDS: 3D-structure;Membrane;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix;Vacuole SUBCELLULAR LOCATION: Vacuole membrane ; Multi-pass membrane protein Q09933,"PROTEIN NAMES: Phosphoprotein p93 ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) FUNCTION: Has a role in sister chromatid separation. KEYWORDS: 3D-structure;Cytoplasm;Cytoskeleton;Microtubule;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body Cytoplasm, cytoskeleton, spindle Note=Spindle pole body during metaphase and spindle microtubules during anaphase. " Q09JZ4,"PROTEIN NAMES: Leucine-rich repeat-containing protein ODA7 (Dynein assembly factor 1, axonemal homolog) (Leucine-rich repeat-containing protein 50 homolog) (Outer row dynein-assembly protein 7) PROTEIN FAMILY: DNAAF1 family ORGANISM: Chlamydomonas reinhardtii (Chlamydomonas smithii) SIMILARITY: Belongs to the DNAAF1 family. FUNCTION: Cilium-specific protein required for cilia structures. Axonemal dynein-associated protein that participates in a structural link between inner and outer row dyneins. KEYWORDS: Cell projection;Cilium;Coiled coil;Cytoplasm;Cytoskeleton;Leucine-rich repeat;Repeat SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, cilium axoneme " Q0C8M4,"PROTEIN NAMES: Dihydromonacolin L monooxygenase LovA (Dihydromonacolin L hydroxylase) (Lovastatin biosynthesis cluster protein A) (Monacolin L hydroxylase) PROTEIN FAMILY: Cytochrome P450 family ORGANISM: Aspergillus terreus (strain NIH 2624 / FGSC A1156) SIMILARITY: Belongs to the cytochrome P450 family. FUNCTION: Dihydromonacolin L monooxygenase; part of the gene cluster that mediates the biosynthesis of lovastatin (also known as mevinolin, mevacor or monacolin K), a hypolipidemic inhibitor of (3S)-hydroxymethylglutaryl-coenzyme A (HMG-CoA) reductase (HMGR). The first step in the biosynthesis of lovastatin is the production of dihydromonacolin L acid by the lovastatin nonaketide synthase lovB and the trans-acting enoyl reductase lovC via condensation of one acetyl-CoA unit and 8 malonyl-CoA units. Dihydromonacolin L acid is released from lovB by the thioesterase lovG. Next, dihydromonacolin L acid is oxidized by the dihydromonacolin L monooxygenase lovA twice to form monacolin J acid. The 2-methylbutyrate moiety of lovastatin is synthesized by the lovastatin diketide synthase lovF via condensation of one acetyl-CoA unit and one malonyl-CoA unit. Finally, the covalent attachment of this moiety to monacolin J acid is catalyzed by the transesterase lovD to yield lovastatin. LovD has broad substrate specificity and can also convert monacolin J to simvastatin using alpha-dimethylbutanoyl-S-methyl-3-mercaptopropionate (DMB-S-MMP) as the thioester acyl donor, and can also catalyze the reverse reaction and function as hydrolase in vitro. LovD has much higher activity with LovF-bound 2-methylbutanoate than with free diketide substrates. PATHWAY: Polyketide biosynthesis; lovastatin biosynthesis. KEYWORDS: Endoplasmic reticulum;Glycoprotein;Heme;Iron;Membrane;Metal-binding;Monooxygenase;Oxidoreductase;Reference proteome;Signal-anchor;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Single-pass membrane protein Endoplasmic reticulum membrane ; Single-pass type II membrane protein " Q0DC89,"PROTEIN NAMES: Peptide methionine sulfoxide reductase B1, chloroplastic (OsMSRB1) (Peptide-methionine (R)-S-oxide reductase) PROTEIN FAMILY: MsrB Met sulfoxide reductase family ORGANISM: Oryza sativa subsp. japonica (Rice) SIMILARITY: Belongs to the MsrB Met sulfoxide reductase family. FUNCTION: Catalyzes the reduction of methionine sulfoxide (MetSO) to methionine in proteins. Involved in abiotic stress response. Plays a protective role against oxidative stress by restoring activity to proteins that have been inactivated by methionine oxidation. MSRB family specifically reduces the MetSO R-enantiomer. KEYWORDS: Chloroplast;Electron transport;Metal-binding;Oxidoreductase;Plastid;Redox-active center;Reference proteome;Transit peptide;Transport;Zinc SUBCELLULAR LOCATION: Plastid, chloroplast MISCELLANEOUS: Lacks the conserved cysteine (here Thr-148) required for the reduction by thioredoxins (TRX) through a dithiol-disulfide exchange involving both redox-active Cys of TRX and MSRB. The reduction mechanism may occur through sulfenic acid formation on the catalytic cysteine (Cys-202)." Q0GGL7,"PROTEIN NAMES: Interleukin-6 (IL-6) PROTEIN FAMILY: IL-6 superfamily ORGANISM: Oncorhynchus mykiss (Rainbow trout) (Salmo gairdneri) SIMILARITY: Belongs to the IL-6 superfamily. FUNCTION: Cytokine with a wide variety of biological functions in immunity, tissue regeneration, and metabolism. Binds to IL6R, then the complex associates to the signaling subunit IL6ST/gp130 to trigger the intracellular IL6-signaling pathway. The interaction with the membrane-bound IL6R and IL6ST stimulates 'classic signaling', whereas the binding of IL6 and soluble IL6R to IL6ST stimulates 'trans-signaling'. Alternatively, 'cluster signaling' occurs when membrane-bound IL6:IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells. KEYWORDS: Acute phase;Cytokine;Disulfide bond;Growth factor;Secreted;Signal SUBCELLULAR LOCATION: Secreted " Q0H8D7,"PROTEIN NAMES: Nibrin homolog (Nijmegen breakage syndrome 1 protein) (AtNbs1) PROTEIN FAMILY: Nibrin family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the Nibrin family. FUNCTION: Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The MRN complex is involved in the repair of DNA double-strand breaks (DSBs) via homologous recombination (HR), an error-free mechanism which primarily occurs during S and G2 phases. The complex (1) mediates the end resection of damaged DNA, which generates proper single-stranded DNA, a key initial steps in HR, and is (2) required for the recruitment of other repair factors and efficient activation of ATM and ATR upon DNA damage (By similarity). The MRN complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11, to initiate end resection, which is required for single-strand invasion and recombination (By similarity). Within the MRN complex, NBS1 acts as a protein-protein adapter, which specifically recognizes and binds phosphorylated proteins, promoting their recruitment to DNA damage sites (By similarity). Recruits MRE11 and RAD50 components of the MRN complex to DSBs in response to DNA damage (By similarity). KEYWORDS: Alternative splicing;Cell cycle;Chromosome;DNA damage;DNA repair;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus Chromosome Note=Localizes to DNA double-strand breaks (DSBs). " Q0J7P4,"PROTEIN NAMES: Nuclear transcription factor Y subunit B-11 (OsNF-YB11) (Protein DAYS TO HEADING 8) (Protein HEADING DATE 5) (Transcriptional activator HAP3H) (OsHAP3H) PROTEIN FAMILY: NFYB/HAP3 subunit family ORGANISM: Oryza sativa subsp. japonica (Rice) SIMILARITY: Belongs to the NFYB/HAP3 subunit family. FUNCTION: Probable transcription factor involved in the regulation of flowering time under long day (LD) conditions. Functions as a repressor of flowering, independently of HD1 and GHD7. Controls flowering time by negatively regulating the expression of EHD1 and HD3A. Regulates plant height by promoting cell elongation in the internodes. Component of the NF-Y/HAP transcription factor complex (By similarity). KEYWORDS: 3D-structure;Cytoplasm;DNA-binding;Flowering;Growth regulation;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus Cytoplasm " Q0JEB7,"PROTEIN NAMES: Transcription factor BHLH6 (Basic helix-loop-helix protein 6) (OsbHLH6) (osbHLH006) (bHLH transcription factor 6) PROTEIN FAMILY: BHLH protein family ORGANISM: Oryza sativa subsp. japonica (Rice) SIMILARITY: Belongs to the bHLH protein family. FUNCTION: Transcription factor involved in phosphate starvation response. Acts as a positive regulator of phosphate homeostasis and phosphate signaling by antagonizing SPX4. Transcription factor involved jasmonate signaling. Regulates shoot growth inhibition in response to jasmonate. May play a role as transcriptional activator in the regulation of jasmonate-mediated stress-inducible gene expression. Possesses transactivation activity in vivo. KEYWORDS: Cytoplasm;DNA-binding;Jasmonic acid signaling pathway;Nucleus;Reference proteome;Stress response;Transcription;Transcription regulation SUBCELLULAR LOCATION: Cytoplasm Nucleus MISCELLANEOUS: Overexpression of BHLH6 promotes shoot and root phosphate accumulation, and root hair growth and acid phosphatase activity under phosphate sufficiency." Q0JHU7,"PROTEIN NAMES: Transcription factor MYB3R-2 (Myb-related protein MYB3R-2) (OsMYB3R-2) ORGANISM: Oryza sativa subsp. japonica (Rice) FUNCTION: Transcription factor involved in abiotic stress responses. May play a regulatory role in tolerance to salt, cold, and drought stresses. Transcriptional activator that binds specifically to a mitosis-specific activator cis-element 5'-(T/C)C(T/C)AACGG(T/C)(T/C)A-3', found in promoters of cyclin genes such as CYCB1-1 and KNOLLE (AC Q84R43). Positively regulates a subset of G2/M phase-specific genes, including CYCB1-1, CYCB2-1, CYCB2-2, and CDC20.1 in response to cold treatment. KEYWORDS: Alternative splicing;DNA-binding;Nucleus;Reference proteome;Repeat;Stress response;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: Plants over-expressing MYB3R-2 show increased tolerance to freezing. Plants over-expressing MYB3R-2 show retarded growth, increased tolerance to cold, drought and salt stresses, and have decreased sensitivity to seed germination inhibition by abscisic acid (ABA) or salt." Q0JLP9,"PROTEIN NAMES: Probable protein phosphatase 2C 6 (OsPP2C06) (ABI1-like protein 1) (OsABI-LIKE1) (OsABIL1) PROTEIN FAMILY: PP2C family ORGANISM: Oryza sativa subsp. japonica (Rice) SIMILARITY: Belongs to the PP2C family. FUNCTION: Probable protein phosphatase that may function in abscisic acid (ABA) signaling. KEYWORDS: 3D-structure;Abscisic acid signaling pathway;Cytoplasm;Hydrolase;Magnesium;Manganese;Metal-binding;Nucleus;Protein phosphatase;Reference proteome SUBCELLULAR LOCATION: Nucleus Cytoplasm, cytosol Note=Localizes predominantly in nucleus. MISCELLANEOUS: Plants overexpressing PP2C06 exhibit dramatically reduced fertility and severe pre-harvest sprouting." Q0K845,"PROTEIN NAMES: Sulfoacetaldehyde dehydrogenase (acylating) PROTEIN FAMILY: Aldehyde dehydrogenase family ORGANISM: Cupriavidus necator (strain ATCC 17699 / DSM 428 / KCTC 22496 / NCIMB 10442 / H16 / Stanier 337) (Ralstonia eutropha) SIMILARITY: Belongs to the aldehyde dehydrogenase family. FUNCTION: Involved in the degradation of sulfoacetate, a widespread natural product. Catalyzes the conversion of sulfoacetyl-CoA and NADPH to sulfoacetaldehyde, CoA and NADP(+). Specific for NADP(+) and sulfoacetaldehyde. KEYWORDS: Cytoplasm;Direct protein sequencing;NADP;Oxidoreductase;Reference proteome SUBCELLULAR LOCATION: Cytoplasm " Q0KHU5,"PROTEIN NAMES: Lysophosphatidylcholine acyltransferase (1-acylglycerophosphocholine O-acyltransferase) (Acyltransferase-like 2) PROTEIN FAMILY: 1-acyl-sn-glycerol-3-phosphate acyltransferase family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family. FUNCTION: Acetyltransferase which mediates the conversion of 1-acyl-sn-glycero-3-phosphocholine (LPC) into phosphatidylcholine (PC) (By similarity). Has a calcium-independent activity (By similarity). Displays a clear preference for saturated fatty acyl-CoAs, and 1-myristoyl or 1-palmitoyl LPC as acyl donors and acceptors, respectively (By similarity). Involved in the regulation of lipid droplet number and size. PATHWAY: Lipid metabolism; phospholipid metabolism. KEYWORDS: Acyltransferase;Calcium;Endoplasmic reticulum;Golgi apparatus;Lipid biosynthesis;Lipid droplet;Lipid metabolism;Membrane;Phospholipid biosynthesis;Phospholipid metabolism;Reference proteome;Repeat;RNA editing;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Single-pass type II membrane protein Golgi apparatus membrane ; Single-pass type II membrane protein Lipid droplet " Q0P5N6,"PROTEIN NAMES: ADP-ribosylation factor-like protein 16 PROTEIN FAMILY: Small GTPase superfamily, Arf family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the small GTPase superfamily. Arf family. FUNCTION: May suppress the RNA sensing activity of RIGI in a GTP-dependent. KEYWORDS: Cytoplasm;GTP-binding;Nucleotide-binding;Reference proteome SUBCELLULAR LOCATION: Cytoplasm " Q0PW40,"PROTEIN NAMES: Cysteine-rich receptor-like protein kinase 13 (Cysteine-rich RLK13) PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family, CRK subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CRK subfamily. KEYWORDS: Alternative splicing;ATP-binding;Glycoprotein;Kinase;Membrane;Nucleotide-binding;Phosphoprotein;Receptor;Reference proteome;Repeat;Serine/threonine-protein kinase;Signal;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Single-pass membrane protein MISCELLANEOUS: [Isoform 2]: May be due to intron retention.; MISCELLANEOUS: [Isoform 3]: May be due to intron retention." Q0V967,"PROTEIN NAMES: F-box only protein 5 (Early mitotic inhibitor 1) (Harpy protein) ORGANISM: Danio rerio (Zebrafish) (Brachydanio rerio) FUNCTION: During embryonic development, regulates the integrity of the genome and therefore the cell cycle progression by preventing rereplication through an APC-Cdh1-dependent mechanism. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Cell cycle;Cell division;Cytoplasm;Metal-binding;Mitosis;Nucleus;Reference proteome;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Cytoplasm " Q0VC00,"PROTEIN NAMES: Phospholipase ABHD3 (Abhydrolase domain-containing protein 3) PROTEIN FAMILY: AB hydrolase superfamily, AB hydrolase 4 family ORGANISM: Bos taurus (Bovine) SIMILARITY: Belongs to the AB hydrolase superfamily. AB hydrolase 4 family. FUNCTION: Phospholipase that may play a role in phospholipids remodeling. May selectively cleave myristate (C14)-containing phosphatidylcholines through its predominant phospholipase 1 activity, cleaving preferentially acyl groups in sn1 position. In parallel, may have a minor phospholipase 2 activity acting on acyl groups in position sn2. In addition to (C14)-containing phosphatidylcholines, may also act on other medium-chain-containing and oxidatively truncated phospholipids. KEYWORDS: Hydrolase;Lipid metabolism;Membrane;Phospholipid metabolism;Reference proteome;Serine esterase;Signal-anchor;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Single-pass type II membrane protein " Q0VCK9,"PROTEIN NAMES: Vacuole membrane protein 1 PROTEIN FAMILY: VMP1 family ORGANISM: Bos taurus (Bovine) SIMILARITY: Belongs to the VMP1 family. FUNCTION: Phospholipid scramblase involved in lipid homeostasis and membrane dynamics processes. Has phospholipid scramblase activity toward cholesterol and phosphatidylserine, as well as phosphatidylethanolamine and phosphatidylcholine. Required for autophagosome formation: participates in early stages of autophagosome biogenesis at the endoplasmic reticulum (ER) membrane by reequilibrating the leaflets of the ER as lipids are extracted by ATG2 (ATG2A or ATG2B) to mediate autophagosome assembly. Regulates ATP2A2 activity to control ER-isolation membrane contacts for autophagosome formation. In addition to autophagy, involved in other processes in which phospholipid scramblase activity is required. Modulates ER contacts with lipid droplets, mitochondria and endosomes. Plays an essential role in formation of cell junctions (By similarity). Upon stress such as bacterial and viral infection, promotes formation of cytoplasmic vacuoles followed by cell death. Involved in the cytoplasmic vacuolization of acinar cells during the early stage of acute pancreatitis (By similarity). KEYWORDS: Acetylation;Autophagy;Cell adhesion;Cell membrane;Endoplasmic reticulum;Lipid transport;Membrane;Reference proteome;Transmembrane;Transmembrane helix;Transport;Vacuole SUBCELLULAR LOCATION: Endoplasmic reticulum-Golgi intermediate compartment membrane ; Multi-pass membrane protein Cell membrane ; Multi-pass membrane protein Vacuole membrane ; Multi-pass membrane protein Endoplasmic reticulum membrane ; Multi-pass membrane protein " Q0VGK4,"PROTEIN NAMES: Lysophospholipase D GDPD1 (Glycerophosphodiester phosphodiesterase 4) (Glycerophosphodiester phosphodiesterase domain-containing protein 1) PROTEIN FAMILY: Glycerophosphoryl diester phosphodiesterase family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the glycerophosphoryl diester phosphodiesterase family. FUNCTION: Hydrolyzes lysoglycerophospholipids to produce lysophosphatidic acid (LPA) and the corresponding amines. Shows a preference for 1-O-alkyl-sn-glycero-3-phosphocholine (lyso-PAF), lysophosphatidylethanolamine (lyso-PE) and lysophosphatidylcholine (lyso-PC). May be involved in bioactive N-acylethanolamine biosynthesis from both N-acyl-lysoplasmenylethanolamin (N-acyl-lysoPlsEt) and N-acyl-lysophosphatidylethanolamin (N-acyl-lysoPE). In addition, hydrolyzes glycerophospho-N-acylethanolamine to N-acylethanolamine. Does not display glycerophosphodiester phosphodiesterase activity, since it cannot hydrolyze either glycerophosphoinositol or glycerophosphocholine. KEYWORDS: Cytoplasm;Endoplasmic reticulum;Hydrolase;Lipid metabolism;Membrane;Metal-binding;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cytoplasm Membrane ; Multi-pass membrane protein Cytoplasm, perinuclear region Endoplasmic reticulum Note=Concentrated at the perinuclear region and the cell periphery. " Q0VZ68,"PROTEIN NAMES: Tyrosine 2,3-aminomutase (Tyrosine ammonia-lyase) PROTEIN FAMILY: TAL/TAM family ORGANISM: Chondromyces crocatus SIMILARITY: Belongs to the TAL/TAM family. FUNCTION: Has aminomutase and, to a lesser extent, ammonia-lyase activity. Primarily, catalyzes the rearrangement of L-tyrosine to R-beta-tyrosine, which is incorporated into secondary metabolites called chondramides. The aminomutase activity mainly produces R-beta-tyrosine but also S-beta tyrosine in smaller amounts. Does not accept D-tyrosine, L-histidine or L-phenylalanine as substrates. KEYWORDS: Isomerase;Lyase MISCELLANEOUS: Chondramides are secondary metabolites with antifungal and cytotoxic activity. They are non-ribosomally produced depsipeptides consisting of a polyketide chain and 3 amino acids (alanine, N-methyltryptophan and beta-tyrosine or alpha-methoxy-beta-tyrosine)." Q0WML0,"PROTEIN NAMES: ABC transporter B family member 27 (ABC transporter ABCB.27) (AtABCB27) (Aluminum tolerance-related ATP-binding cassette transporter) (Antigen peptide transporter-like 2) (Transporter associated with antigen processing-like protein 2) (AtTAP2) PROTEIN FAMILY: ABC transporter superfamily, ABCB family, Multidrug resistance exporter subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily. FUNCTION: Probably involved in redistribution of internalized aluminum. May mediate vacuolar sequestration of a metal complex. KEYWORDS: ATP-binding;Membrane;Nucleotide-binding;Reference proteome;Transmembrane;Transmembrane helix;Transport;Vacuole SUBCELLULAR LOCATION: Vacuole membrane ; Multi-pass membrane protein " Q0WNY5,"PROTEIN NAMES: Wall-associated receptor kinase-like 18 PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. FUNCTION: Serine/threonine-protein kinase that may function as a signaling receptor of extracellular matrix component. KEYWORDS: ATP-binding;Disulfide bond;Glycoprotein;Kinase;Membrane;Nucleotide-binding;Phosphoprotein;Reference proteome;Serine/threonine-protein kinase;Signal;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Single-pass type I membrane protein " Q0WPH8,"PROTEIN NAMES: Serine/threonine-protein kinase Nek5 (NimA-related protein kinase 5) (AtNEK6) (AtNek5) PROTEIN FAMILY: Protein kinase superfamily, NEK Ser/Thr protein kinase family, NIMA subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily. FUNCTION: Involved in epidermal-cell morphogenesis in hypocotyls and roots. May act on the microtubule function. May have a secondary role in trichome branching. KEYWORDS: ATP-binding;Kinase;Nucleotide-binding;Reference proteome;Serine/threonine-protein kinase;Transferase " Q0WQ75,"PROTEIN NAMES: Vacuolar protein sorting-associated protein 51 homolog (Protein UNHINGED) PROTEIN FAMILY: VPS51 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the VPS51 family. FUNCTION: Acts as a component of the GARP complex that is involved in retrograde transport from early and late endosomes to the trans-Golgi network (TGN). The GARP complex is required for the maintenance of protein retrieval from endosomes to the TGN, acid hydrolase sorting, lysosome function, endosomal cholesterol traffic and autophagy. VPS51 participates in retrograde transport of acid hydrolase receptors, likely by promoting tethering and SNARE-dependent fusion of endosome-derived carriers to the TGN. Acts as a component of the EARP complex that is involved in endocytic recycling. The EARP complex associates with Rab4-positive endosomes and promotes recycling of internalized transferrin receptor (TFRC) to the plasma membrane (By similarity). Required for vacuolar targeting and cellular trafficking. Involved in the regulation of vascular tissue patterning, probably by regulating PIN1 expression pattern, thus modulating auxin flux. Important to prevent PIN1 accumulation within margin cells, possibly by targeting PIN1 to the lytic vacuole. Regulates PIN1 and ATHB8 expression pattern in secondary veins. KEYWORDS: Acetylation;Endosome;Golgi apparatus;Lipid transport;Protein transport;Reference proteome;Transport SUBCELLULAR LOCATION: Golgi apparatus, trans-Golgi network Recycling endosome Prevacuolar compartment Note=Localizes to the trans-Golgi network as part of the GARP complex, while it localizes to recycling endosomes as part of the EARP complex. " Q0WQE7,"PROTEIN NAMES: AUGMIN subunit 3 PROTEIN FAMILY: HAUS3 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the HAUS3 family. FUNCTION: Involved in microtubules reorganization during spindle and phragmoplast development. Required for gamma-tubulin localization during mitosis. KEYWORDS: Cell cycle;Cell division;Coiled coil;Cytoplasm;Cytoskeleton;Microtubule;Mitosis;Reference proteome SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, spindle Cytoplasm, cytoskeleton, phragmoplast Note=Preferentially localizes to microtubules minus ends. " Q0WQF4,"PROTEIN NAMES: Vacuolar protein sorting-associated protein 53 A (AtVPS53) (Protein HEAT-INTOLERANT 1) PROTEIN FAMILY: VPS53 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the VPS53 family. FUNCTION: Acts as a component of the GARP complex that is involved in retrograde transport from early and late endosomes to the trans-Golgi network (TGN). The GARP complex facilitates tethering as well as SNARE complex assembly at the Golgi (By similarity). Required for vesicle trafficking involved in plasma membrane protein composition. Probably involved in pollen tube elongation and other polar growth. Confers basal tolerance to long-term heat stress and osmotic stress, by acclimation of the plasma membrane. KEYWORDS: Alternative splicing;Coiled coil;Cytoplasm;Endosome;Golgi apparatus;Membrane;Protein transport;Reference proteome;Transport SUBCELLULAR LOCATION: Cytoplasm Golgi apparatus membrane ; Peripheral membrane protein Golgi apparatus, trans-Golgi network membrane ; Peripheral membrane protein Endosome membrane ; Peripheral membrane protein Note=Localized in the GARP complex in the Golgi and post-Golgi compartments. " Q0WRJ7,"PROTEIN NAMES: Peptidyl-prolyl cis-trans isomerase FKBP20-2, chloroplastic (PPIase FKBP20-2) (FK506-binding protein 20-2) (AtFKBP20-2) (Immunophilin FKBP20-2) (Rotamase) PROTEIN FAMILY: FKBP-type PPIase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the FKBP-type PPIase family. FUNCTION: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides (By similarity). Involved in the accumulation of the PSII complex. KEYWORDS: Chloroplast;Direct protein sequencing;Disulfide bond;Isomerase;Plastid;Reference proteome;Rotamase;Thylakoid;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid lumen MISCELLANEOUS: PPIase activity is unaffected on reduction." Q0WSH6,"PROTEIN NAMES: Pentatricopeptide repeat-containing protein At4g14850 (Protein LOVASTATIN INSENSITIVE 1) PROTEIN FAMILY: PPR family, PCMP-H subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the PPR family. PCMP-H subfamily. FUNCTION: Acts as a regulatory factor of isoprenoid biosynthesis. Could bind RNA. KEYWORDS: Nucleotide-binding;Reference proteome;Repeat;RNA-binding " Q0WSN2,"PROTEIN NAMES: Protein DA1-related 2 (Protein LATERAL ROOT DEVELOPMENT 3) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Acts redundantly with DA1 and DAR1 to regulate endoreduplication during leaf development. Together with DA1 and DAR1, modulates the protein stability of the transcription factors TCP14 and TCP15, which repress endoreduplication by directly regulating the expression of cell-cycle genes. Involved in root phloem development. Is an essential component of early phloem development, long-distance delivery of phloem content, and proper maintenance of root system architecture. Involved in the control of root meristem size. Functions genetically downstream of cytokinin and IAA3 to maintain normal auxin distribution by influencing polar auxin transport. Acts through the PLETHORA pathway, upstream of PLT1 and PLT2 to influence root stem cell niche activity and thus control root meristem size. KEYWORDS: LIM domain;Metal-binding;Reference proteome;Repeat;Ubl conjugation;Zinc MISCELLANEOUS: Exogenous treatment with auxin rescues the phloem defects of the lrd3 knockout mutant." Q0WT48,PROTEIN NAMES: DnaJ protein ERDJ2A (Chaperone protein dnaJ 21) (AtDjC21) (AtJ21) (Endoplasmic reticulum dnaJ domain-containing protein 2A) (AtERdj2A) (Translocation protein SEC63 homolog ERDJ2A) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Required for integral membrane and secreted preprotein translocation across the endoplasmic reticulum membrane. KEYWORDS: Alternative splicing;Chaperone;Endoplasmic reticulum;Glycoprotein;Membrane;Protein transport;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein Q0WUA3,"PROTEIN NAMES: 2-carboxy-1,4-naphthoquinone phytyltransferase, chloroplastic (1,4-dihydroxy-2-naphthoate phytyltransferase) (1,4-dihydroxy-2-naphthoate polyprenyltransferase) (Protein ABERRANT CHLOROPLAST DEVELOPMENT 4) (menA-like protein) (AtMENA) PROTEIN FAMILY: MenA family, Type 2 subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the MenA family. Type 2 subfamily. FUNCTION: Involved in the synthesis of phylloquinone (vitamin K1). Catalyzes the transfer of a prenyl chain to 2-carboxy-1,4-naphthoquinone. KEYWORDS: Alternative splicing;Chloroplast;Membrane;Plastid;Reference proteome;Transferase;Transit peptide;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Plastid, chloroplast membrane ; Multi-pass membrane protein " Q0WVM7,"PROTEIN NAMES: Probable transcriptional regulator SLK1 (AtSLK1) (Protein SEUSS-like 1) PROTEIN FAMILY: Adn1/SEU family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the adn1/SEU family. FUNCTION: Probable transcription regulator that functions in the development of the carpel margin meristem similarly to SEUSS (SEU). In association with SEU, supports organ development from meristematic regions by facilitating auxin response and thus organ initiation, and by sustaining meristematic potential through the maintenance of PHABULOSA expression. DNA-binding adapter subunit of the SEU-SLK1 transcriptional corepressor of abiotic stress (e.g. salt and osmotic stress) response genes. KEYWORDS: Developmental protein;Differentiation;Flowering;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " Q0WW17,"PROTEIN NAMES: Protein REDUCED WALL ACETYLATION 2 PROTEIN FAMILY: PC-esterase family, CASD1 subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the PC-esterase family. CASD1 subfamily. FUNCTION: Probable O-acetyltransferase involved in the acetylation of cell wall polymers (both pectic and nonpectic polysaccharides) and of xylan during secondary wall biosynthesis. Catalyzes the O-acetylation of xyloglucan.; FUNCTION: Seems required for infection by the necrotrophic fungal pathogen Botrytis cinerea. KEYWORDS: Alternative splicing;Endoplasmic reticulum;Glycoprotein;Golgi apparatus;Membrane;Oxidoreductase;Phosphoprotein;Reference proteome;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus membrane ; Multi-pass membrane protein Endoplasmic reticulum membrane ; Multi-pass membrane protein " Q10074,"PROTEIN NAMES: Vacuolar amino acid transporter 3 PROTEIN FAMILY: Amino acid/polyamine transporter 2 family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the amino acid/polyamine transporter 2 family. FUNCTION: Involved in amino acid efflux from the vacuole to the cytoplasm. Capable of transporting large neutral amino acids including tyrosine, glutamine, asparagine, isoleucine and leucine (By similarity). Required for spore formation. KEYWORDS: Amino-acid transport;Endoplasmic reticulum;Membrane;Phosphoprotein;Reference proteome;Sporulation;Transmembrane;Transmembrane helix;Transport;Vacuole SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein Vacuole membrane ; Multi-pass membrane protein " Q10128,"PROTEIN NAMES: Interleukin cytokine receptor-related protein 2 (Interleukin-17 receptor-like protein 2) ORGANISM: Caenorhabditis elegans FUNCTION: Forms a receptor complex together with receptor ilcr-1, which upon activation acts as a modulator of neuronal activity. Binding of the ligand ilc-17.1 to the ilcr-1/2 receptor complex triggers a signaling cascade that activates the downstream signaling components actl-1, pik-1 and nfki-1, and results in increased neuronal activity in RMG interneurons in response to input from oxygen-sensing neurons. This leads to increased animal movement and promotes aggregation behavior. KEYWORDS: Cell membrane;Glycoprotein;Membrane;Receptor;Reference proteome;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein " Q10134,"PROTEIN NAMES: Iron-sensing transcriptional repressor (Transcription factor gaf2) (Gaf-2) ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) FUNCTION: Transcriptional repressor that binds the consensus promoter sequence 5'-[AT]GATAA-3' during iron-replete conditions to down-regulate transcription of target genes. Represses the expression of the iron transporter fio1 in response to high iron concentrations. Also represses the expression of str1, str2 and str3. Represses the expression of shu1 in presence of iron. KEYWORDS: DNA-binding;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Repeat;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus " Q10146,"PROTEIN NAMES: Exosome complex exonuclease rrp6 (Ribosomal RNA-processing protein 6) PROTEIN FAMILY: Exosome component 10/RRP6 family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the exosome component 10/RRP6 family. FUNCTION: Nuclear-specific catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and cryptic unstable transcripts (CUTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. RRP6 has 3'-5' exonuclease activity which is not modulated upon association with Exo-9 suggesting that the complex inner RNA-binding path is not used to access its active site (By similarity). Involved in the processing of 5.8S rRNA, a subset of mRNAs and non-coding RNAs. Plays mostly a structural role in degrading some of its mRNA targets, such as early meiotic mRNAs bound to mmi1 and iron homeostasis mRNAs, probably by the channeling of these transcripts to dis3 for degradation. KEYWORDS: Exonuclease;Exosome;Hydrolase;Manganese;Metal-binding;Nuclease;Nucleus;Reference proteome;RNA-binding;rRNA processing;Zinc SUBCELLULAR LOCATION: Nucleus, nucleolus " Q10156,"PROTEIN NAMES: Dual specificity protein kinase lkh1 PROTEIN FAMILY: Protein kinase superfamily, CMGC Ser/Thr protein kinase family, Lammer subfamily ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. Lammer subfamily. FUNCTION: Protein kinase that may act as a negative regulator of filamentous growth and flocculation. Appears to have a role in normal cell wall and septum formation and in cell separation. May have antagonistic function in the regulation of beta-glucan distribution between the sites for cell wall and septum assembly. KEYWORDS: ATP-binding;Kinase;Nucleotide-binding;Phosphoprotein;Reference proteome;Serine/threonine-protein kinase;Transferase;Tyrosine-protein kinase " Q10198,"PROTEIN NAMES: Kinetochore protein ndc80 (NMS complex subunit ndc80) PROTEIN FAMILY: NDC80/HEC1 family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the NDC80/HEC1 family. FUNCTION: Acts as a component of the NMS (Ndc80-MIND-Spc7) super complex which has a role in kinetochore function during late meiotic prophase and throughout the mitotic cell cycle. Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. KEYWORDS: Cell cycle;Cell division;Centromere;Chromosome;Coiled coil;Kinetochore;Meiosis;Mitosis;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus. Chromosome, centromere, kinetochore. Note=Associated with kinetochores. " Q10199,"PROTEIN NAMES: Septation protein imp2 ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) FUNCTION: Required for normal septation. Involved in the disassembly of the medial ring during septation. KEYWORDS: 3D-structure;Cell cycle;Cell division;Coiled coil;Cytoplasm;Cytoskeleton;Mitosis;Phosphoprotein;Reference proteome;SH3 domain SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton Note=Associates with the medial ring during septation. " Q10256,PROTEIN NAMES: UBA domain-containing protein Mud1 (DNA-damage-inducible protein DDI1 homolog) (UBA domain-containing protein 1) PROTEIN FAMILY: DDI1 family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the DDI1 family. FUNCTION: Recognizes and binds polyubiquitin chains. Acts as a linker between the 19S proteasome and polyubiquitinated proteins via UBA domain interactions with ubiquitin for their subsequent degradation. Aspartic protease. Appears to act as negative regulator of constitutive exocytosis. May act at the level of secretory vesicle docking and fusion as a competitive inhibitor of SNARE assembly. Required for S-phase checkpoint control (By similarity). KEYWORDS: 3D-structure;Aspartyl protease;Cell membrane;Cytoplasm;Hydrolase;Membrane;Protease;Protein transport;Reference proteome;Transport SUBCELLULAR LOCATION: Cytoplasm Cell membrane ; Peripheral membrane protein ; Cytoplasmic side Q10282,"PROTEIN NAMES: Guanine nucleotide-binding protein subunit beta PROTEIN FAMILY: WD repeat G protein beta family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the WD repeat G protein beta family. FUNCTION: Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction. Required for adenylate cyclase activation. KEYWORDS: Cytoplasm;Nucleus;Reference proteome;Repeat;Sporulation;Transducer;WD repeat SUBCELLULAR LOCATION: Cytoplasm Nucleus " Q10356,PROTEIN NAMES: Glutathione-independent glyoxalase DJ-1 (Heat shock protein 31 homolog 6) (Protein DJ-1 homolog) PROTEIN FAMILY: Peptidase C56 family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the peptidase C56 family. FUNCTION: Catalyzes the conversion of methylglyoxal (MG) to D-lactate in a single glutathione (GSH)-independent step. May play a role in detoxifying endogenously produced glyoxals. Involved in protection against reactive oxygen species (ROS) (By similarity). KEYWORDS: 3D-structure;Cytoplasm;Lyase;Nucleus;Reference proteome;Stress response SUBCELLULAR LOCATION: Cytoplasm Nucleus Q10364,"PROTEIN NAMES: Serine/threonine-protein kinase sck2 PROTEIN FAMILY: Protein kinase superfamily, AGC Ser/Thr protein kinase family, PKC subfamily ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. FUNCTION: Protein kinase that is part of growth control pathway which is at least partially redundant with the cAMP pathway. KEYWORDS: ATP-binding;Kinase;Nucleotide-binding;Phosphoprotein;Reference proteome;Serine/threonine-protein kinase;Transferase " Q10423,"PROTEIN NAMES: Cruciform cutting endonuclease 1, mitochondrial (Protein ydc2) ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) FUNCTION: Capable of resolving Holliday junctions. Specific for 4-way junctions. Seems to be important for the maintenance of mitochondrial DNA. Cleaves fixed junctions at the point of strand exchange. Cleaves after 5'-CT-3' and 5'-TT-3' sequences. KEYWORDS: 3D-structure;Endonuclease;Hydrolase;Magnesium;Metal-binding;Mitochondrion;Nuclease;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Mitochondrion " Q10425,"PROTEIN NAMES: Eukaryotic translation initiation factor 3 subunit B (eIF3b) (Eukaryotic translation initiation factor 3 90 kDa subunit homolog) (eIF3 p90) (Translation initiation factor eIF3 p90 subunit homolog) (spPrt1) PROTEIN FAMILY: EIF-3 subunit B family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the eIF-3 subunit B family. FUNCTION: RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation. KEYWORDS: Coiled coil;Cytoplasm;Initiation factor;Phosphoprotein;Protein biosynthesis;Reference proteome;Repeat;RNA-binding;WD repeat SUBCELLULAR LOCATION: Cytoplasm " Q10432,"PROTEIN NAMES: Coiled-coil quantitatively-enriched protein 1 (Structural maintenance of chromosomes protein ccq1) (SMC protein ccq1) ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) FUNCTION: Component of the meiotic bouquet that facilitates meiotic nuclear reorganization of the telomeres to the centrosome. Links telomeres to the meiotic centrosome component pcp1. Essential for the formation of normal telomere clusters during meiotic prophase. Required for telomere length regulation and chromosome segregation. Required for proper positioning of nucleosomes at heterochromatic loci and for transcriptional gene silencing (TGS) function of the Snf2/Hdac-containing repressor complex (SHREC). KEYWORDS: 3D-structure;Chromosome;Chromosome partition;Coiled coil;Direct protein sequencing;Nucleus;Reference proteome;Telomere SUBCELLULAR LOCATION: Nucleus, nucleoplasm. Chromosome, telomere. Note=Associates with major heterochromatin, sub-telomeres, rDNA and the mat locus. " Q10484,"PROTEIN NAMES: Vesicle-associated membrane protein-associated protein scs22 (VAMP-associated protein scs22) PROTEIN FAMILY: VAMP-associated protein (VAP) family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the VAMP-associated protein (VAP) (TC 9.B.17) family. FUNCTION: Vesicle-associated membrane protein-associated protein (VAP) implicated in maintaining the cortical endoplasmic reticulum (ER)-plasma membrane (PM) attachment. ER-PM contacts function to modulate the distribution of contractile ring components to ensure robust ring assembly. ER-PM contacts function also in controlling exocytosis and maintenance of cell polarity regulating cell shape. VAPs play an important role in regulating eisosome assembly. VAPs also contribute to ER-phagy by tethering atg8 to the ER membrane, but also by maintaining the ER-plasma membrane contact (Ref.7). KEYWORDS: Endoplasmic reticulum;Membrane;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Single-pass type IV membrane protein Note=Localizes at the cortical endoplasmic reticulum-plasma membrane contact sites. " Q10651,"PROTEIN NAMES: Amyloid-beta-like protein PROTEIN FAMILY: APP family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the APP family. FUNCTION: Required for normal developmental progression throughout all life stages. Specifically required for the molt stage during all larval transitions and morphogenesis. Acts with heterochronic genes, including members of the let-7 family, to regulate larval stage to adult transition. Acts synergistically with acn-1 in let-7 regulated postembryonic cell division of hypodermal seam cells. Acts in multiple pathways to influence daf-12 and daf-16 activity to in turn regulate physiological and reproductive processes such as body size and egg-laying. May play a role in neurotransmission. KEYWORDS: 3D-structure;Alternative splicing;Amyloid;Developmental protein;Differentiation;Disulfide bond;Endosome;Glycoprotein;Membrane;Neurogenesis;Reference proteome;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Single-pass type I membrane protein Early endosome MISCELLANEOUS: Lacks conserved metal-binding sites and has only weak affinity for copper in vitro." Q10916,"PROTEIN NAMES: Sphingomyelin phosphodiesterase 1 (Acid sphingomyelinase 1) (ASM-1) PROTEIN FAMILY: Acid sphingomyelinase family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the acid sphingomyelinase family. FUNCTION: Sphingomyelin phosphodiesterase (sphingomyelinase) that converts sphingomyelin to ceramide (N-acyl-sphingoid base) and phosphocholine at acidic pH. Displays its enzymatic activity when secreted. May play distinct roles in signaling. PATHWAY: Lipid metabolism; sphingolipid metabolism. KEYWORDS: Disulfide bond;Glycoprotein;Glycosidase;Hydrolase;Lipid metabolism;Metal-binding;Reference proteome;Secreted;Signal;Sphingolipid metabolism;Zinc SUBCELLULAR LOCATION: Secreted MISCELLANEOUS: There are two types of sphingomyelinases: asm (acid), and nsm (neutral). Only acid sphingomyelinases have been found in worms.; MISCELLANEOUS: Does not require zinc ions as a cofactor." Q10988,PROTEIN NAMES: UV-damage endonuclease (UVDE) PROTEIN FAMILY: Uve1/UvsE family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the uve1/UvsE family. FUNCTION: Endonuclease for the repair of UV-irradiated DNA. Involved in the excision of cyclobutane pyrimidine dimers (CPD) and 6-4 pyrimidine pyrimidones (6-4PP) which forms the UV damage repair (UVDR) pathway. Functions also in oxidative damage repair in vivo. Provides back-up AP endonuclease activity to apn2 together with apn1. KEYWORDS: DNA damage;DNA excision;DNA repair;Endonuclease;Hydrolase;Nuclease;Reference proteome Q10990,"PROTEIN NAMES: Cell division cycle protein cdt2 PROTEIN FAMILY: WD repeat cdt2 family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the WD repeat cdt2 family. FUNCTION: Substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for DNA replication during mitosis and meiosis. The DCX(DTL) complex, also named CRL4(CDT2) complex, mediates the polyubiquitination and subsequent degradation of cdt1 and spd1. Involved in the regulation of mitotic and pre-meiotic S-phase progression. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Cell cycle;Cell division;Meiosis;Mitosis;Nucleus;Reference proteome;Repeat;Ubl conjugation pathway;WD repeat SUBCELLULAR LOCATION: Nucleus " Q10B19,"PROTEIN NAMES: tRNA:m(4)X modification enzyme TRM13 (tRNA methylase 13) (OsTRM13) PROTEIN FAMILY: Methyltransferase TRM13 family ORGANISM: Oryza sativa subsp. japonica (Rice) SIMILARITY: Belongs to the methyltransferase TRM13 family. FUNCTION: tRNA methylase that catalyzes 2'-O-methyladenosine (Am) nucleoside formation on tRNA(Gly)(GCC) in vitro. May 2'-O-methylate cytidine(4) in tRNA(Pro) and tRNA(Gly)(GCC), and adenosine(4) in tRNA(His). Involved in salt stress tolerance. KEYWORDS: Cytoplasm;Metal-binding;Methyltransferase;Nucleus;Reference proteome;S-adenosyl-L-methionine;Stress response;Transferase;tRNA processing;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Cytoplasm MISCELLANEOUS: Seedlings subjected to salt stress and abscisic acid (ABA) treatment exhibit a dramatic increase of 2'-O-methyladenosine (Am) nucleoside. Plants over-expressing TRM13 show improved salt stress tolerance, and plant silencing TRM13 display decreased stress tolerance." Q10F03,"PROTEIN NAMES: Protein FLOURY ENDOSPERM 6, chloroplastic (SKIPa-interacting protein 4) ORGANISM: Oryza sativa subsp. japonica (Rice) FUNCTION: Involved in compound starch granule formation and starch synthesis in endosperm. May act as a regulatory scaffolding protein and affect starch synthesis and compound starch granule formation through direct interaction with isoamylase 1 (ISA1). Binds starch, amylopectin and amylose through its C-terminal carbohydrate-binding domain (CBM) in vitro. KEYWORDS: Chloroplast;Coiled coil;Plastid;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast Note=Localizes to granule-like structures in chloroplasts. " Q10M74,"PROTEIN NAMES: Guanylate kinase 2, chloroplastic/mitochondrial (OsGKpm) (GMP kinase 2) (Protein VIRESCENT 2) PROTEIN FAMILY: Guanylate kinase family ORGANISM: Oryza sativa subsp. japonica (Rice) SIMILARITY: Belongs to the guanylate kinase family. FUNCTION: Essential for recycling GMP and indirectly, cGMP. Essential for chloroplast differentiation at early stage of leaf development. May not be involved in the synthesis and maintenance of the organellar DNA during leaf development. KEYWORDS: ATP-binding;Chloroplast;Kinase;Mitochondrion;Nucleotide-binding;Plastid;Reference proteome;Transferase;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast. Mitochondrion. " Q10QA5,"PROTEIN NAMES: Strigolactone esterase D14 (Protein DWARF 14) (Protein DWARF 88) (Protein HIGH-TILLERING DWARF 2) PROTEIN FAMILY: AB hydrolase superfamily ORGANISM: Oryza sativa subsp. japonica (Rice) SIMILARITY: Belongs to the AB hydrolase superfamily. FUNCTION: Involved in strigolactone (SL) signaling pathway. May function downstream of SL synthesis, as a component of hormone signaling or as an enzyme that participates in the conversion of SL to the bioactive form. Strigolactones are hormones that inhibit tillering and shoot branching through the MAX-dependent pathway, contribute to the regulation of shoot architectural response to phosphate-limiting conditions and function as rhizosphere signal that stimulates hyphal branching of arbuscular mycorrhizal fungi and trigger seed germination of root parasitic weeds. Strigolactone-dependent association of D14 with D3 and D53 (a repressor of SL signaling) triggers D53 ubiquitination and degradation. Hydrolyzes the butenolide ring of SLs. A reaction product D-OH is trapped in the cavity of D14, inducing the interaction with SLR1, and probably with other proteins such as D3 and D53. Contributes to the negative regulation of gibberellin signaling. KEYWORDS: 3D-structure;Cytoplasm;Hydrolase;Nucleus;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Nucleus Note=The interaction between D14 and SLR1 takes place in the nucleus. The interaction between D14 and D3 takes place in the nucleus. " Q10S44,PROTEIN NAMES: Transcription factor BHLH3 (Basic helix-loop-helix protein 3) (OsbHLH003) (Protein RAC IMMUNITY 1) (bHLH transcription factor 3) PROTEIN FAMILY: BHLH protein family ORGANISM: Oryza sativa subsp. japonica (Rice) SIMILARITY: Belongs to the bHLH protein family. FUNCTION: Transcription factor involved in defense responses that functions downstream of RAC1 and upstream of PAL1 and WRKY19 genes. KEYWORDS: Cytoplasm;DNA-binding;Nucleus;Plant defense;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus Cytoplasm Note=Localizes mainly in the nucleus. Q11098,"PROTEIN NAMES: Tetraspanin-17 PROTEIN FAMILY: Tetraspanin (TM4SF) family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the tetraspanin (TM4SF) family. FUNCTION: Protects dopaminergic neurons against oxidative stress-induced neurodegeneration. May act partly via dopamine receptor dop-2 to negatively regulate dopamine reuptake transporter dat-1 activity. Also plays a role in modulating behaviors linked to dopamine signaling. Confers protection against oxidative stress in the whole body. KEYWORDS: Alternative splicing;Cell membrane;Cell projection;Membrane;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Cell projection, dendrite Cell projection, axon Note=May localize near the nucleus or ER. " Q11131,"PROTEIN NAMES: Alpha-(1,3)-fucosyltransferase 7 (Fucosyltransferase 7) (Fucosyltransferase VII) (Fuc-TVII) (FucT-VII) (Galactoside 3-L-fucosyltransferase) PROTEIN FAMILY: Glycosyltransferase 10 family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the glycosyltransferase 10 family. FUNCTION: Catalyzes the transfer of L-fucose, from a guanosine diphosphate-beta-L-fucose, to the N-acetyl glucosamine (GlcNAc) of a distal alpha2,3 sialylated lactosamine unit of a glycoprotein or a glycolipid-linked sialopolylactosamines chain through an alpha-1,3 glycosidic linkage and participates in the final fucosylation step in the biosynthesis of the sialyl Lewis X (sLe(x)), a carbohydrate involved in cell and matrix adhesion during leukocyte trafficking and fertilization. In vitro, also synthesizes sialyl-dimeric-Lex structures, from VIM-2 structures and both di-fucosylated and trifucosylated structures from mono-fucosylated precursors (By similarity). However does not catalyze alpha 1-3 fucosylation when an internal alpha 1-3 fucosylation is present in polylactosamine chain and the fucosylation rate of the internal GlcNAc residues is reduced once fucose has been added to the distal GlcNAc (By similarity). Also catalyzes the transfer of a fucose from GDP-beta-fucose to the 6-sulfated a(2,3)sialylated substrate to produce 6-sulfo sLex mediating significant L-selectin-dependent cell adhesion. Through sialyl-Lewis(x) biosynthesis, can control SELE- and SELP-mediated cell adhesion with leukocytes and allows leukocytes tethering and rolling along the endothelial tissue thereby enabling the leukocytes to accumulate at a site of inflammation. May enhance embryo implantation through sialyl Lewis X (sLeX)-mediated adhesion of embryo cells to endometrium (By similarity). May affect insulin signaling by up-regulating the phosphorylation and expression of some signaling molecules involved in the insulin-signaling pathway through SLe(x) which is present on the glycans of the INSRR alpha subunit (By similarity). PATHWAY: Protein modification; protein glycosylation. KEYWORDS: Alternative splicing;Disulfide bond;Glycoprotein;Glycosyltransferase;Golgi apparatus;Membrane;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Note=Membrane-bound form in trans cisternae of Golgi. " Q12001,"PROTEIN NAMES: Dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase (Asparagine-linked glycosylation protein 6) (Dol-P-Glc:Man(9)GlcNAc(2)-PP-Dol alpha-1,3-glucosyltransferase) (Dolichyl-P-Glc:Man9GlcNAc2-PP-dolichyl glucosyltransferase) PROTEIN FAMILY: ALG6/ALG8 glucosyltransferase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the ALG6/ALG8 glucosyltransferase family. FUNCTION: Dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. In the lumen of the endoplasmic reticulum, adds the first glucose residue from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide intermediate Man(9)GlcNAc(2)-PP-Dol to produce Glc(1)Man(9)GlcNAc(2)-PP-Dol. PATHWAY: Protein modification; protein glycosylation. KEYWORDS: 3D-structure;Endoplasmic reticulum;Glycosyltransferase;Membrane;Reference proteome;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein " Q12003,"PROTEIN NAMES: Serine/threonine-protein kinase ENV7 (Late endosome and vacuole interface protein 7) PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. FUNCTION: Serine/threonine-protein kinase involved in vacuolar processing and morphology. KEYWORDS: ATP-binding;Kinase;Lipoprotein;Membrane;Nucleotide-binding;Palmitate;Reference proteome;Serine/threonine-protein kinase;Transferase;Vacuole SUBCELLULAR LOCATION: Vacuole membrane ; Peripheral membrane protein " Q12006,"PROTEIN NAMES: Palmitoyltransferase PFA4 (Protein S-acyltransferase) (PAT) (Protein fatty acyltransferase 4) PROTEIN FAMILY: DHHC palmitoyltransferase family, PFA4 subfamily ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the DHHC palmitoyltransferase family. PFA4 subfamily. FUNCTION: Mediates the reversible addition of palmitate to target proteins, thereby regulating their membrane association and biological function. Palmitoylates several amino acid permeases. Palmitoylates chitin synthase CHS3, which is required for its proper export from the ER. Can palmitoylate RAS2 in vitro. KEYWORDS: Acyltransferase;Endoplasmic reticulum;Lipoprotein;Membrane;Palmitate;Reference proteome;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein " Q12010,PROTEIN NAMES: Vacuolar lysine transporter YPQ1 (PQ-loop repeat-containing protein 1) PROTEIN FAMILY: Laat-1 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the laat-1 family. FUNCTION: Amino acid transporter that moves lysine into the vacuole. May also contribute to low affinity arginine import into the vacuole. Has also been suggested to mediate export of cationic amino acids from the vacuole. May function as an amino acid/proton antiporter. KEYWORDS: Amino-acid transport;Glycoprotein;Membrane;Reference proteome;Repeat;Transmembrane;Transmembrane helix;Transport;Vacuole SUBCELLULAR LOCATION: Vacuole membrane ; Multi-pass membrane protein MISCELLANEOUS: Present with 1800 molecules/cell in log phase SD medium. Q12017,PROTEIN NAMES: Phosducin-like protein 2 (Viral IAP-associated factor 1 homolog) PROTEIN FAMILY: Phosducin family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the phosducin family. FUNCTION: Essential for cell growth. Inhibits early G-protein signaling events following pheromone stimulation. Inhibits the folding activity of the chaperonin-containing T-complex (CCT) CCT2 which leads to inhibition of cytoskeletal actin folding. Plays a role in cell cycle progression in G1/S phase. KEYWORDS: 3D-structure;Cytoplasm;Pheromone response;Phosphoprotein;Reference proteome;Signal transduction inhibitor SUBCELLULAR LOCATION: Cytoplasm MISCELLANEOUS: Present with 7700 molecules/cell in log phase SD medium. Q12046,"PROTEIN NAMES: Pre-mRNA-splicing factor CWC2 (Complexed with CEF1 protein 2) (PRP19-associated complex protein 40) (Synthetic lethal with CLF1 protein 3) PROTEIN FAMILY: RRM CWC2 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the RRM CWC2 family. FUNCTION: Involved in the first step of pre-mRNA splicing. Required for cell growth and cell cycle control. Plays a role in the levels of the U1, U4, U5 and U6 snRNAs and the maintenance of the U4/U6 snRNA complex. May provide the link between the 'nineteen complex' NTC spliceosome protein complex and the spliceosome through the U6 snRNA. Associates predominantly with U6 snRNAs in assembled active spliceosomes. Binds directly to the internal stem-loop (ISL) domain of the U6 snRNA and to the pre-mRNA intron near the 5' splice site during the activation and catalytic phases of the spliceosome cycle. Binds also to U1, U4, U5 and U6 snRNAs and to pre-mRNAs, in vitro. Is not required for the Prp2-mediated remodeling of the activated spliceosome. KEYWORDS: 3D-structure;Cell cycle;Metal-binding;mRNA processing;mRNA splicing;Nucleus;Phosphoprotein;Reference proteome;RNA-binding;Spliceosome;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: Present with 2650 molecules/cell in log phase SD medium." Q12051,"PROTEIN NAMES: Geranylgeranyl pyrophosphate synthase BTS1 (GGPP synthase) (GGPPSase) ((2E,6E)-farnesyl diphosphate synthase) (BET2 suppressor protein 1) (Dimethylallyltranstransferase) (Farnesyl diphosphate synthase) (Farnesyltranstransferase) (Geranylgeranyl diphosphate synthase) (Geranyltranstransferase) PROTEIN FAMILY: FPP/GGPP synthase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the FPP/GGPP synthase family. FUNCTION: Catalyzes the trans-addition of the 3 molecules of IPP onto DMAPP to form geranylgeranyl pyrophosphate. Required for the membrane attachment of YPT1 and SEC4. May be involved in vesicle trafficking and protein sorting. PATHWAY: Isoprenoid biosynthesis; farnesyl diphosphate biosynthesis; farnesyl diphosphate from geranyl diphosphate and isopentenyl diphosphate: step 1/1.; PATHWAY: Isoprenoid biosynthesis; geranyl diphosphate biosynthesis; geranyl diphosphate from dimethylallyl diphosphate and isopentenyl diphosphate: step 1/1.; PATHWAY: Isoprenoid biosynthesis; geranylgeranyl diphosphate biosynthesis; geranylgeranyl diphosphate from farnesyl diphosphate and isopentenyl diphosphate: step 1/1. KEYWORDS: 3D-structure;Carotenoid biosynthesis;Cytoplasm;Isoprene biosynthesis;Magnesium;Metal-binding;Protein transport;Reference proteome;Transferase;Transport SUBCELLULAR LOCATION: Cytoplasm MISCELLANEOUS: Present with 2840 molecules/cell in log phase SD medium." Q12052,"PROTEIN NAMES: Trimethylguanosine synthase (Cap-specific guanine-N2 methyltransferase) (snRNA/snoRNA cap hypermethylase) PROTEIN FAMILY: Methyltransferase superfamily, Trimethylguanosine synthase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the methyltransferase superfamily. Trimethylguanosine synthase family. FUNCTION: Catalyzes the two serial methylation steps for the conversion of the 7-monomethylguanosine (m(7)G) caps of snRNAs and snoRNAs to a 2,2,7-trimethylguanosine (m(2,2,7)G) cap structure. The enzyme is specific for guanine, and N7 methylation must precede N2 methylation. Hypermethylates the m3G cap on TLC1 telomerase which affects telomere silencing and telomere length regulation. Required for pre-mRNA splicing, pre-rRNA processing and small ribosomal subunit synthesis. Involved in nucleolar structural organization. KEYWORDS: Methyltransferase;Nucleus;Reference proteome;S-adenosyl-L-methionine;Transferase SUBCELLULAR LOCATION: Nucleus, nucleolus " Q12060,"PROTEIN NAMES: Transcriptional coactivator HFI1/ADA1 ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Functions as a component of the transcription regulatory histone acetylation (HAT) complexes SAGA, SALSA and SLIK. SAGA is involved in RNA polymerase II-dependent transcriptional regulation of approximately 10% of yeast genes. At the promoters, SAGA is required for recruitment of the basal transcription machinery. It influences RNA polymerase II transcriptional activity through different activities such as TBP interaction (SPT3, SPT8 and SPT20) and promoter selectivity, interaction with transcription activators (GCN5, ADA2, ADA3 and TRA1), and chromatin modification through histone acetylation (GCN5) and deubiquitination (UBP8). SAGA acetylates nucleosomal histone H3 to some extent (to form H3K9ac, H3K14ac, H3K18ac and H3K23ac). SAGA interacts with DNA via upstream activating sequences (UASs). SALSA, an altered form of SAGA, may be involved in positive transcriptional regulation. SLIK is proposed to have partly overlapping functions with SAGA. It preferentially acetylates methylated histone H3, at least after activation at the GAL1-10 locus. HFI1/ADA1 and SPT20/ADA5 may recruit TATA binding protein (TBP) and possibly other basal factors to bind to the TATA box. KEYWORDS: 3D-structure;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: Present with 7950 molecules/cell in log phase SD medium." Q12071,"PROTEIN NAMES: Vacuolar protein sorting-associated protein 54 (CPF1 genetically-interacting protein 1) (Temperature-sensitive clathrin synthetic mutation protein 3) PROTEIN FAMILY: VPS54 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the VPS54 family. FUNCTION: Involved in retrograde transport from early and late endosomes to late Golgi by linking the vesicle through the t-SNARE TGL1 to the Golgi, leading to the membrane fusion between late Golgi and endosomal vesicles. Seems also to be involved in protein transport from Golgi to the plasma membrane and is required for the integrity of the actin cytoskeleton. KEYWORDS: Coiled coil;Endosome;Golgi apparatus;Membrane;Mitochondrion;Phosphoprotein;Protein transport;Reference proteome;Transport SUBCELLULAR LOCATION: Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein. Endosome membrane; Peripheral membrane protein. Mitochondrion membrane ; Peripheral membrane protein Note=May also be mitochondrial. MISCELLANEOUS: Present with 2540 molecules/cell in log phase SD medium." Q12094,"PROTEIN NAMES: 18S rRNA aminocarboxypropyltransferase (20S rRNA accumulation protein 3) (ScTsr3) PROTEIN FAMILY: TDD superfamily, TSR3 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the TDD superfamily. TSR3 family. FUNCTION: Aminocarboxypropyltransferase that catalyzes the aminocarboxypropyl transfer on pseudouridine at position 1191 (Psi1191) in 18S rRNA. It constitutes the last step in biosynthesis of the hypermodified N1-methyl-N3-(3-amino-3-carboxypropyl) pseudouridine (m1acp3-Psi) conserved in eukaryotic 18S rRNA. Required for processing 35S pre-rRNA at site D. KEYWORDS: Cytoplasm;Nucleus;Phosphoprotein;Reference proteome;Ribosome biogenesis;rRNA processing;S-adenosyl-L-methionine;Transferase SUBCELLULAR LOCATION: Cytoplasm Nucleus MISCELLANEOUS: Present with 3610 molecules/cell in log phase SD medium." Q12100,"PROTEIN NAMES: Probable serine/threonine-protein kinase RTK1 (Ribosome biogenesis and tRNA synthetase-associated kinase 1) PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. FUNCTION: Probable serine/threonine-protein kinase that may be involved in ribosome biogenesis. KEYWORDS: ATP-binding;Isopeptide bond;Kinase;Nucleotide-binding;Phosphoprotein;Reference proteome;Serine/threonine-protein kinase;Transferase;Ubl conjugation MISCELLANEOUS: Present with 861 molecules/cell in log phase SD medium." Q12106,PROTEIN NAMES: Mitochondrial adapter protein MCP1 (MDM10-complementing protein 1) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Recruits the lipid transfer protein Vps13 to mitochondria thereby promoting vacuole-mitochondria contacts. Involved in mitochondrial lipid homeostasis. KEYWORDS: Membrane;Mitochondrion;Mitochondrion outer membrane;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Mitochondrion outer membrane ; Multi-pass membrane protein Q12118,PROTEIN NAMES: Small glutamine-rich tetratricopeptide repeat-containing protein 2 (SGT/UBP) (Viral protein U-binding protein) PROTEIN FAMILY: SGT family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the SGT family. FUNCTION: Co-chaperone that binds to the molecular chaperone Hsp70 (SSA1 and SSA2). Regulates Hsp70 ATPase activity (By similarity). Required for recovery from heat shock. KEYWORDS: 3D-structure;Chaperone;Cytoplasm;Direct protein sequencing;Phosphoprotein;Reference proteome;Repeat;TPR repeat SUBCELLULAR LOCATION: Cytoplasm MISCELLANEOUS: Present with 9424 molecules/cell in log phase SD medium. Q12178,"PROTEIN NAMES: Cytosine deaminase (yCD) (Cytosine aminohydrolase) (Fluorocytosine resistance protein 1) PROTEIN FAMILY: Cytidine and deoxycytidylate deaminase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the cytidine and deoxycytidylate deaminase family. FUNCTION: Catalyzes the hydrolytic deamination of cytosine to uracil or 5-methylcytosine to thymine. Is involved in the pyrimidine salvage pathway, which allows the cell to utilize cytosine for pyrimidine nucleotide synthesis. PATHWAY: Pyrimidine metabolism; UMP biosynthesis via salvage pathway; uracil from cytosine: step 1/1. KEYWORDS: 3D-structure;Cytoplasm;Hydrolase;Metal-binding;Nucleus;Reference proteome;Zinc SUBCELLULAR LOCATION: Cytoplasm Nucleus MISCELLANEOUS: Present with 5180 molecules/cell in log phase SD medium." Q12198,"PROTEIN NAMES: Homocysteine/cysteine synthase (Hydrogen sulfide utilizing 1) (O-acetylserine/O-acetylhomoserine sulfhydrylase) (OAS-OAH SHLase) (OAS-OAH sulfhydrylase) PROTEIN FAMILY: Trans-sulfuration enzymes family, MET7 subfamily ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the trans-sulfuration enzymes family. MET7 subfamily. FUNCTION: Plays a role in inorganic sulfur assimilation during sulfur-limited conditions; catalyzes the conversion of O-acetyl-L-homoserine (OAH) into homocysteine in the methionine biosynthesis pathway. Also catalyzes the conversion of O-acetylserine (OAS) into cysteine, the last step in the cysteine biosynthesis pathway. However, it seems that in S.cerevisiae cysteine biosynthesis occurs exclusively through the cystathionine pathway and not via direct incorporation of sulfur into OAS (By similarity). It therefore has no metabolic role in cysteine biosynthesis and may only have a regulatory role controlling OAS levels (By similarity). PATHWAY: Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homocysteine from O-acetyl-L-homoserine. KEYWORDS: Amino-acid biosynthesis;Cytoplasm;Methionine biosynthesis;Pyridoxal phosphate;Reference proteome;Transferase SUBCELLULAR LOCATION: Cytoplasm MISCELLANEOUS: Present with 2070 molecules/cell in log phase SD medium." Q12206,PROTEIN NAMES: Transcriptional modulator WTM2 ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Transcriptional modulator with roles in meiotic regulation and silencing. KEYWORDS: Meiosis;Reference proteome;Repeat;Transcription;Transcription regulation;WD repeat MISCELLANEOUS: Present with 3750 molecules/cell in log phase SD medium. Q12216,PROTEIN NAMES: E3 SUMO-protein ligase SIZ2 (E3 SUMO-protein transferase SIZ2) (SAP and Miz-finger domain-containing protein 2) PROTEIN FAMILY: PIAS family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the PIAS family. FUNCTION: May act as an E3 ligase mediating SUMO/Smt3 attachment to septins. May be involved in chromosome maintenance. PATHWAY: Protein modification; protein sumoylation. KEYWORDS: 3D-structure;Metal-binding;Nucleus;Reference proteome;Transferase;Ubl conjugation;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: Present with 3170 molecules/cell in log phase SD medium. Q12220,"PROTEIN NAMES: U3 small nucleolar RNA-associated protein 12 (U3 snoRNA-associated protein 12) (DOM34-interacting protein 2) (U three protein 12) PROTEIN FAMILY: WD repeat WDR3/UTP12 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the WD repeat WDR3/UTP12 family. FUNCTION: Involved in nucleolar processing of pre-18S ribosomal RNA. KEYWORDS: 3D-structure;Nucleus;Reference proteome;Repeat;Ribonucleoprotein;Ribosome biogenesis;rRNA processing;WD repeat SUBCELLULAR LOCATION: Nucleus, nucleolus MISCELLANEOUS: Present with 9620 molecules/cell in log phase SD medium." Q12232,PROTEIN NAMES: Uncharacterized protein SLP1 (SUN-like protein 1) PROTEIN FAMILY: SLP1 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the SLP1 family. FUNCTION: May be involved in membrane protein folding. Required for localization of MPS3 to the nuclear envelope. KEYWORDS: Endoplasmic reticulum;Glycoprotein;Membrane;Reference proteome;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Single-pass type I membrane protein MISCELLANEOUS: Present with 3250 molecules/cell in log phase SD medium. Q12250,PROTEIN NAMES: 26S proteasome regulatory subunit RPN5 (Proteasome non-ATPase subunit 5) PROTEIN FAMILY: Proteasome subunit p55 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the proteasome subunit p55 family. FUNCTION: Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins. KEYWORDS: 3D-structure;Acetylation;Direct protein sequencing;Proteasome;Reference proteome MISCELLANEOUS: Present with 5710 molecules/cell in log phase SD medium. Q12256,"PROTEIN NAMES: Polyamine transporter 4 PROTEIN FAMILY: Major facilitator superfamily, DHA1 family, Polyamines/proton antiporter subfamily ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the major facilitator superfamily. DHA1 family. Polyamines/proton antiporter (TC 2.A.1.2.16) subfamily. FUNCTION: Cell membrane polyamine/proton antiporter, involved in the detoxification of excess polyamines in the cytoplasm. Recognizes spermidine, spermine and the antimalarial drug quinidine, but not quinine, chloroquine and mefloquine. KEYWORDS: Antiport;Cell membrane;Membrane;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein MISCELLANEOUS: Present with 21400 molecules/cell in log phase SD medium." Q12265,"PROTEIN NAMES: Ribose-phosphate pyrophosphokinase 5 (Phosphoribosyl pyrophosphate synthase 5) PROTEIN FAMILY: Ribose-phosphate pyrophosphokinase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the ribose-phosphate pyrophosphokinase family. FUNCTION: 5-phosphoribose 1-diphosphate synthase involved in nucleotide, histidine, and tryptophan biosynthesis. Active in heteromultimeric complexes with other 5-phosphoribose 1-diphosphate synthases (PRS2, PRS3, PRS4 and PRS5). PATHWAY: Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1. KEYWORDS: ATP-binding;Cytoplasm;Kinase;Magnesium;Metal-binding;Nucleotide biosynthesis;Nucleotide-binding;Phosphoprotein;Reference proteome;Transferase SUBCELLULAR LOCATION: Cytoplasm MISCELLANEOUS: Present with 3310 molecules/cell in log phase SD medium." Q12315,"PROTEIN NAMES: mRNA export factor GLE1 (Nuclear pore protein GLE1) (Nucleoporin GLE1) (RNA export factor GLE1) PROTEIN FAMILY: GLE1 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the GLE1 family. FUNCTION: Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. It is specifically involved in a terminal step of poly(A)+ mRNA transport through the NPC probably by binding the ATP-dependent RNA helicase DBP5 and GFD1 at the cytoplasmic side of the NPC. These interactions are thought to be important for the dissociation of transport proteins such as the heterogeneous nuclear ribonucleoprotein (hnRNP) NAB2 from exported mRNA. KEYWORDS: 3D-structure;Coiled coil;Membrane;mRNA processing;mRNA transport;Nuclear pore complex;Nucleus;Protein transport;Reference proteome;Translocation;Transport SUBCELLULAR LOCATION: Nucleus, nuclear pore complex Nucleus membrane ; Peripheral membrane protein ; Cytoplasmic side Nucleus membrane ; Peripheral membrane protein ; Nucleoplasmic side Note=Biased towards cytoplasmic side. MISCELLANEOUS: Present with 1040 molecules/cell in log phase SD medium." Q12324,PROTEIN NAMES: Calcium channel YVC1 (TRP homolog) (Yeast vacuolar conductance protein 1) PROTEIN FAMILY: Transient receptor family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the transient receptor (TC 1.A.4) family. FUNCTION: Required for release of calcium ions from the vacuole in response to hyperosmotic shock. KEYWORDS: 3D-structure;Calcium;Calcium channel;Calcium transport;Ion channel;Ion transport;Membrane;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix;Transport;Vacuole SUBCELLULAR LOCATION: Vacuole membrane ; Multi-pass membrane protein MISCELLANEOUS: Present with 1310 molecules/cell in log phase SD medium. Q12344,PROTEIN NAMES: GTPase-activating protein GYP5 PROTEIN FAMILY: GYP5 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the GYP5 family. FUNCTION: GTPase-activating protein which accelerates the GTP hydrolysis rate of YPT1 and SEC4. Involved in ER to Golgi trafficking and polarized exocytosis. KEYWORDS: Coiled coil;Cytoplasm;ER-Golgi transport;Exocytosis;GTPase activation;Phosphoprotein;Protein transport;Reference proteome;Transport SUBCELLULAR LOCATION: Cytoplasm. Bud. Bud neck. MISCELLANEOUS: Present with 1940 molecules/cell in log phase SD medium. Q12377,"PROTEIN NAMES: 26S proteasome regulatory subunit RPN6 (Proteasome non-ATPase subunit 4) PROTEIN FAMILY: Proteasome subunit S9 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the proteasome subunit S9 family. FUNCTION: Component of the lid subcomplex of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. In the complex, RPN6 is required for proteasome assembly. KEYWORDS: 3D-structure;Acetylation;Direct protein sequencing;Proteasome;Reference proteome MISCELLANEOUS: Present with 16800 molecules/cell in log phase SD medium." Q12383,"PROTEIN NAMES: tRNA:m(4)X modification enzyme TRM13 (tRNA methylase 13) PROTEIN FAMILY: Methyltransferase TRM13 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the methyltransferase TRM13 family. FUNCTION: tRNA methylase which 2'-O-methylates cytidine(4) in tRNA(Pro) and tRNA(Gly)(GCC), and adenosine(4) in tRNA(His). KEYWORDS: Cytoplasm;Metal-binding;Methyltransferase;Nucleus;Reference proteome;S-adenosyl-L-methionine;Transferase;tRNA processing;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cytoplasm Nucleus MISCELLANEOUS: Present with 1230 molecules/cell in log phase SD medium." Q12396,PROTEIN NAMES: Protein EMP46 (46 kDa endomembrane protein) PROTEIN FAMILY: EMP46/EMP47 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the EMP46/EMP47 family. FUNCTION: Involved in the secretion of glycoproteins and in nucleus architecture and gene silencing. KEYWORDS: 3D-structure;Direct protein sequencing;Disulfide bond;Endoplasmic reticulum;Golgi apparatus;Lectin;Membrane;Metal-binding;Potassium;Protein transport;Reference proteome;Signal;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Golgi apparatus membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Q12404,PROTEIN NAMES: Protein disulfide-isomerase MPD1 PROTEIN FAMILY: Protein disulfide isomerase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the protein disulfide isomerase family. FUNCTION: Participates in the folding of proteins containing disulfide bonds. KEYWORDS: 3D-structure;Disulfide bond;Endoplasmic reticulum;Glycoprotein;Isomerase;Redox-active center;Reference proteome;Signal SUBCELLULAR LOCATION: Endoplasmic reticulum lumen MISCELLANEOUS: Present with 830 molecules/cell in log phase SD medium. Q12417,PROTEIN NAMES: Pre-mRNA-splicing factor PRP46 (Complexed with CEF1 protein 1) (PRP nineteen-associated complex protein 50) (PRP19-associated complex protein 50) (Pre-mRNA-processing protein 46) PROTEIN FAMILY: WD repeat PRL1/PRL2 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the WD repeat PRL1/PRL2 family. FUNCTION: Involved in pre-mRNA splicing. May also be required for cell cycle progression at G2/M (By similarity). KEYWORDS: 3D-structure;Cytoplasm;Direct protein sequencing;mRNA processing;mRNA splicing;Nucleus;Reference proteome;Repeat;Spliceosome;WD repeat SUBCELLULAR LOCATION: Cytoplasm Nucleus MISCELLANEOUS: Present with 1770 molecules/cell in log phase SD medium. Q12421,"PROTEIN NAMES: Autophagy-related protein 31 (CIK1 suppressor protein 1) (Protein CIS1) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Plays a role in starvation-induced autophagy. Involved in mitophagy. Functions with ATG17 and ATG29 at the preautophagosomal structure (PAS) in order to form normal autophagosomes under starvation conditions. May be involved in microtubule function, such as chromosome segregation and karyogamy. KEYWORDS: Autophagy;Chromosome partition;Cytoplasm;Cytoskeleton;Karyogamy;Microtubule;Phosphoprotein;Protein transport;Reference proteome;Transport SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton Preautophagosomal structure. MISCELLANEOUS: Present with 486 molecules/cell in log phase SD medium." Q12425,PROTEIN NAMES: Iron-sulfur assembly protein 2 PROTEIN FAMILY: HesB/IscA family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the HesB/IscA family. FUNCTION: Involved in the assembly of mitochondrial and cytoplasmic iron-sulfur proteins. Probably involved in the binding of an intermediate of Fe/S cluster assembly. KEYWORDS: Iron;Metal-binding;Mitochondrion;Reference proteome SUBCELLULAR LOCATION: Mitochondrion matrix. MISCELLANEOUS: Present with 1560 molecules/cell in log phase SD medium. Q12445,"PROTEIN NAMES: Nucleoporin POM34 (Nuclear pore protein POM34) (Pore membrane protein POM34) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. KEYWORDS: 3D-structure;Membrane;mRNA transport;Nuclear pore complex;Nucleus;Phosphoprotein;Protein transport;Reference proteome;Translocation;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Nucleus, nuclear pore complex Nucleus membrane; Multi-pass membrane protein. Note=Central core structure of the nuclear pore complex. " Q12446,PROTEIN NAMES: Proline-rich protein LAS17 ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) KEYWORDS: 3D-structure;Phosphoprotein;Reference proteome MISCELLANEOUS: Present with 8580 molecules/cell in log phase SD medium. Q12449,"PROTEIN NAMES: Hsp90 co-chaperone AHA1 (Activator of Hsp90 ATPase protein 1) PROTEIN FAMILY: AHA1 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the AHA1 family. FUNCTION: Co-chaperone that binds to the molecular chaperone HSP82 and stimulates its ATPase activity. Binding to HSP82 promotes a conformational switch in the catalytic loop of HSP82, facilitating the interaction of the catalytic 'Arg-380' with ATP in the N-terminal nucleotide-binding domain. Although not essential, it confers thermotolerance when intracellular levels of HSP82 are limiting. KEYWORDS: 3D-structure;Chaperone;Cytoplasm;Reference proteome;Stress response SUBCELLULAR LOCATION: Cytoplasm MISCELLANEOUS: Present with 13939 molecules/cell in log phase SD medium." Q12457,"PROTEIN NAMES: RNA polymerase I termination factor (NTS1 silencing protein 1) ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUNCTION: DNA-binding protein that recognizes sequence-specific replication termini (Ter sites) within rDNA. Binds to rDNA terminator elements and mediates efficient RNA polymerase I transcription termination. Required for rDNA silencing at the non-transcribed spacer 1 (NTS1). Promotes the association of SIR2 with NTS1 and contributes to maintenance of rDNA stability. KEYWORDS: DNA-binding;Nucleus;Phosphoprotein;Reference proteome;Repeat SUBCELLULAR LOCATION: Nucleus, nucleolus MISCELLANEOUS: Present with 432 molecules/cell in log phase SD medium." Q12462,PROTEIN NAMES: Peroxisomal membrane protein PMP27 (Peroxin-11) PROTEIN FAMILY: Peroxin-11 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the peroxin-11 family. FUNCTION: Involved in peroxisomal proliferation. Promotes peroxisome division and biogenesis. KEYWORDS: Direct protein sequencing;Membrane;Peroxisome;Peroxisome biogenesis;Reference proteome SUBCELLULAR LOCATION: Peroxisome membrane ; Peripheral membrane protein MISCELLANEOUS: Present with 1630 molecules/cell in log phase SD medium. Q12476,"PROTEIN NAMES: Protein AIR2 (Arginine methyltransferase-interacting RING finger protein 2) PROTEIN FAMILY: AIR1 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the AIR1 family. FUNCTION: Component of the TRAMP (TRF4) complex which has a poly(A) RNA polymerase activity and is involved in a post-transcriptional quality control mechanism limiting inappropriate expression of genetic information. Polyadenylation is required for the degradative activity of the exosome on several of its nuclear RNA substrates like cryptic transcripts generated by RNA polymerase II and III, or hypomethylated pre-tRNAi-Met. Both complexes polyadenylate RNA processing and degradation intermediates of snRNAs, snoRNAs and mRNAs that accumulate in strains lacking a functional exosome. AIR2 also inhibits the methylation of NPL3 mediated by HMT1 through its interaction with HMT1. KEYWORDS: 3D-structure;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Repeat;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: Present with 1800 molecules/cell in log phase SD medium." Q12483,"PROTEIN NAMES: Vacuolar-sorting protein SNF8 (ESCRT-II complex subunit VPS22) (Vacuolar protein-sorting-associated protein 22) PROTEIN FAMILY: SNF8 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the SNF8 family. FUNCTION: Component of the endosomal sorting complex required for transport II (ESCRT-II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. KEYWORDS: 3D-structure;Coiled coil;Cytoplasm;Endosome;Membrane;Protein transport;Reference proteome;Transport SUBCELLULAR LOCATION: Cytoplasm Endosome membrane ; Peripheral membrane protein MISCELLANEOUS: Present with 1040 molecules/cell in log phase SD medium." Q12502,"PROTEIN NAMES: Protein LDB19 (Low dye-binding protein 19) PROTEIN FAMILY: LDB19 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the LDB19 family. FUNCTION: May be involved in protein-linked oligosaccharide phosphorylation since the deletion reduces the negative charge of the cell surface. Involved in the resistance to EDTA, cadmium chloride, cycloheximide, 6-dimethylaminopurine, methyl caffeate, beta-chloro-L-alanine, caffeine and cerulenin. KEYWORDS: Cell cycle;Cytoplasm;Golgi apparatus;Isopeptide bond;Phosphoprotein;Reference proteome;Ubl conjugation SUBCELLULAR LOCATION: Cytoplasm Golgi apparatus MISCELLANEOUS: Present with 295 molecules/cell in log phase SD medium." Q12505,"PROTEIN NAMES: Serine/threonine-protein kinase SKS1 (Suppressor kinase of SNF3) PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. FUNCTION: May have a role in glucose regulation. KEYWORDS: ATP-binding;Kinase;Nucleotide-binding;Reference proteome;Serine/threonine-protein kinase;Transferase MISCELLANEOUS: Present with 2810 molecules/cell in log phase SD medium." Q12509,"PROTEIN NAMES: Actin-like protein ARP6 PROTEIN FAMILY: Actin family, ARP6 subfamily ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the actin family. ARP6 subfamily. FUNCTION: Component of the SWR1 complex which mediates the ATP-dependent exchange of histone H2A for the H2A variant HZT1 leading to transcriptional regulation of selected genes by chromatin remodeling. Involved in chromosome stability. KEYWORDS: 3D-structure;Activator;Chromatin regulator;Cytoplasm;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Cytoplasm Nucleus MISCELLANEOUS: Present with 238 molecules/cell in log phase SD medium." Q12511,"PROTEIN NAMES: [Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 1, mitochondrial (PDP 1) (Phosphatase two C protein 5) (Protein phosphatase 2C homolog 5) (PP2C-5) (Protein phosphatase of PDH protein 1) (Pyruvate dehydrogenase complex phosphatase 1) (PDC phosphatase 1) PROTEIN FAMILY: PP2C family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the PP2C family. FUNCTION: Catalyzes the dephosphorylation and concomitant reactivation of the E1 alpha subunit (PDA1) of the pyruvate dehydrogenase complex. KEYWORDS: Hydrolase;Magnesium;Manganese;Metal-binding;Mitochondrion;Protein phosphatase;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Mitochondrion intermembrane space MISCELLANEOUS: Present with 7550 molecules/cell in log phase SD medium." Q12514,"PROTEIN NAMES: General negative regulator of transcription subunit 5 PROTEIN FAMILY: CNOT2/3/5 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the CNOT2/3/5 family. FUNCTION: Acts as a component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover. The NOT protein subcomplex negatively regulates the basal and activated transcription of many genes. Preferentially affects TC-type TATA element-dependent transcription. Could directly or indirectly inhibit component(s) of the general transcription machinery. KEYWORDS: 3D-structure;Activator;Coiled coil;Cytoplasm;Isopeptide bond;Nucleus;Phosphoprotein;Reference proteome;Repressor;Transcription;Transcription regulation;Ubl conjugation SUBCELLULAR LOCATION: Cytoplasm Nucleus. MISCELLANEOUS: Present with 5110 molecules/cell in log phase SD medium." Q12517,"PROTEIN NAMES: mRNA-decapping enzyme subunit 1 PROTEIN FAMILY: DCP1 family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the DCP1 family. FUNCTION: Component of the decapping complex necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Decapping is the major pathway of mRNA degradation in yeast. It occurs through deadenylation, decapping and subsequent 5' to 3' exonucleolytic decay of the transcript body. DCP1 is activated by the DEAD-box helicase DHH1 and destabilizes the eIF-4F cap-binding complex from the mRNA. KEYWORDS: 3D-structure;Cytoplasm;Direct protein sequencing;mRNA processing;Nonsense-mediated mRNA decay;Phosphoprotein;Reference proteome;RNA-binding SUBCELLULAR LOCATION: Cytoplasm, P-body Note=Is concentrated in several cytoplasmic foci called P bodies (or cytoplasmic processing bodies) which represent sites of mRNA decapping and 5' to 3' exonucleotidic decay. MISCELLANEOUS: Present with 2880 molecules/cell in log phase SD medium." Q12524,"PROTEIN NAMES: Peroxisomal coenzyme A diphosphatase 1, peroxisomal (Pcd1p) (8-oxo-dGTP/2-hydroxy-dATP diphosphatase) PROTEIN FAMILY: Nudix hydrolase family, PCD1 subfamily ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the Nudix hydrolase family. PCD1 subfamily. FUNCTION: Diphosphatase (pyrophosphatase) with specificity for coenzyme A and CoA derivatives. Catalyzes the hydrolysis of the diphosphate linkage in CoA to give 3',5'-ADP and 4'-phosphopantetheine. Prefers oxidized CoA disulfide (CoASSCoA) over CoA as a substrate. May be required to remove potentially toxic oxidized CoA disulfide from peroxisomes to maintain the capacity for beta-oxidation of fatty acids. Can also hydrolyze 8-oxo-dGTP and 2-OH-dATP in vitro; therefore it may function as a sanitizing enzyme for oxidized nucleotides and may contribute to prevention of spontaneous mutagenesis due to the misincorporation of these oxidized nucleotides during DNA synthesis. Shows moderate activity in vitro with several short chain acyl-CoA esters and very low activity on 3'-dephospho-CoA while is not active with (deoxy)nucleoside 5'-triphosphates, nucleoside 5'-di- or monophosphates, diadenosine polyphosphates, nucleoside 5'-diphosphosugars, cytidine 5'-diphosphoalcohols, NAD(+), NADH, or FAD. KEYWORDS: Direct protein sequencing;Hydrolase;Magnesium;Manganese;Metal-binding;Peroxisome;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Peroxisome MISCELLANEOUS: Present with 238 molecules/cell in log phase SD medium." Q12797,"PROTEIN NAMES: Aspartyl/asparaginyl beta-hydroxylase (Aspartate beta-hydroxylase) (ASP beta-hydroxylase) (Peptide-aspartate beta-dioxygenase) PROTEIN FAMILY: Aspartyl/asparaginyl beta-hydroxylase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the aspartyl/asparaginyl beta-hydroxylase family. FUNCTION: [Isoform 1]: Specifically hydroxylates an Asp or Asn residue in certain epidermal growth factor-like (EGF) domains of a number of proteins.; FUNCTION: [Isoform 8]: Membrane-bound Ca(2+)-sensing protein, which is a structural component of the ER-plasma membrane junctions. Isoform 8 regulates the activity of Ca(+2) released-activated Ca(+2) (CRAC) channels in T-cells. KEYWORDS: 3D-structure;Alternative splicing;Calcium;Dioxygenase;Disease variant;Disulfide bond;Endoplasmic reticulum;Glycoprotein;Iron;Membrane;Metal-binding;Oxidoreductase;Phosphoprotein;Reference proteome;Repeat;Sarcoplasmic reticulum;Signal-anchor;TPR repeat;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: [Isoform 1]: Endoplasmic reticulum membrane; Single-pass type II membrane protein.; SUBCELLULAR LOCATION: [Isoform 4]: Sarcoplasmic reticulum membrane; Single-pass type II membrane protein.; SUBCELLULAR LOCATION: [Isoform 8]: Endoplasmic reticulum membrane; Single-pass type II membrane protein " Q12815,PROTEIN NAMES: Tastin (Trophinin-assisting protein) (Trophinin-associated protein) ORGANISM: Homo sapiens (Human) FUNCTION: Could be involved with bystin and trophinin in a cell adhesion molecule complex that mediates an initial attachment of the blastocyst to uterine epithelial cells at the time of the embryo implantation. KEYWORDS: Alternative splicing;Cell adhesion;Cytoplasm;Phosphoprotein;Reference proteome;Repeat SUBCELLULAR LOCATION: Cytoplasm. Q12834,"PROTEIN NAMES: Cell division cycle protein 20 homolog (p55CDC) PROTEIN FAMILY: WD repeat CDC20/Fizzy family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the WD repeat CDC20/Fizzy family. FUNCTION: Involved in the metaphase/anaphase transition of cell cycle. Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and confers substrate specificity upon the complex. Is regulated by MAD2L1: in metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. CDC20-APC/C-induced degradation of NEUROD2 induces presynaptic differentiation. The CDC20-APC/C complex promotes proper dilation formation and radial migration by degrading CCDC41 (By similarity). PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: 3D-structure;Acetylation;Cell cycle;Cell division;Centromere;Chromosome;Cytoplasm;Cytoskeleton;Differentiation;Disease variant;Isopeptide bond;Kinetochore;Mitosis;Neurogenesis;Phosphoprotein;Reference proteome;Repeat;Ubl conjugation;Ubl conjugation pathway;WD repeat SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Chromosome, centromere, kinetochore Cytoplasm, cytoskeleton, spindle pole " Q12948,"PROTEIN NAMES: Forkhead box protein C1 (Forkhead-related protein FKHL7) (Forkhead-related transcription factor 3) (FREAC-3) ORGANISM: Homo sapiens (Human) FUNCTION: DNA-binding transcriptional factor that plays a role in a broad range of cellular and developmental processes such as eye, bones, cardiovascular, kidney and skin development. Acts either as a transcriptional activator or repressor. Binds to the consensus binding site 5'-[G/C][A/T]AAA[T/C]AA[A/C]-3' in promoter of target genes. Upon DNA-binding, promotes DNA bending. Acts as a transcriptional coactivator. Stimulates Indian hedgehog (Ihh)-induced target gene expression mediated by the transcription factor GLI2, and hence regulates endochondral ossification (By similarity). Acts also as a transcriptional coregulator by increasing DNA-binding capacity of GLI2 in breast cancer cells. Regulates FOXO1 through binding to a conserved element, 5'-GTAAACAAA-3' in its promoter region, implicating FOXC1 as an important regulator of cell viability and resistance to oxidative stress in the eye. Cooperates with transcription factor FOXC2 in regulating expression of genes that maintain podocyte integrity (By similarity). Promotes cell growth inhibition by stopping the cell cycle in the G1 phase through TGFB1-mediated signals. Involved in epithelial-mesenchymal transition (EMT) induction by increasing cell proliferation, migration and invasion. Involved in chemokine CXCL12-induced endothelial cell migration through the control of CXCR4 expression (By similarity). Plays a role in the gene regulatory network essential for epidermal keratinocyte terminal differentiation. Essential developmental transcriptional factor required for mesoderm-derived tissues, such as the somites, skin, bone and cartilage. Positively regulates CXCL12 and stem cell factor expression in bone marrow mesenchymal progenitor cells, and hence plays a role in the development and maintenance of mesenchymal niches for haematopoietic stem and progenitor cells (HSPC). Plays a role in corneal transparency by preventing both blood vessel and lymphatic vessel growth during embryonic development in a VEGF-dependent manner. Involved in chemokine CXCL12-induced endothelial cell migration through the control of CXCR4 expression (By similarity). May function as a tumor suppressor. KEYWORDS: Activator;Angiogenesis;Deafness;Developmental protein;Disease variant;DNA-binding;Nucleus;Peters anomaly;Phosphoprotein;Reference proteome;Repressor;Transcription;Transcription regulation;Ubl conjugation SUBCELLULAR LOCATION: Nucleus Note=Colocalizes with PITX2 isoform 3 in the nucleus at subnuclear chromatine regions. Colocalizes with CBX5 to a heterochromatin-rich region of the nucleus. Colocalizes with GLI2 in the nucleus (By similarity). " Q12XX5,"PROTEIN NAMES: Glucosyl-3-phosphoglycerate/mannosyl-3-phosphoglycerate phosphatase (GpgP) (MpgP) PROTEIN FAMILY: HAD-like hydrolase superfamily, MPGP family ORGANISM: Methanococcoides burtonii (strain DSM 6242 / NBRC 107633 / OCM 468 / ACE-M) SIMILARITY: Belongs to the HAD-like hydrolase superfamily. MPGP family. FUNCTION: Involved in the biosynthesis of glucosylglycerate. Catalyzes the dephosphorylation of glucosyl-3-phosphoglycerate (GPG) and mannosyl-3-phosphoglycerate (MPG) to glucosylglycerate (GG) and mannosylglycerate (MG), respectively. KEYWORDS: Hydrolase;Magnesium;Metal-binding " Q13105,"PROTEIN NAMES: Zinc finger and BTB domain-containing protein 17 (Myc-interacting zinc finger protein 1) (Miz-1) (Zinc finger protein 151) (Zinc finger protein 60) PROTEIN FAMILY: Krueppel C2H2-type zinc-finger protein family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein family. FUNCTION: Transcription factor that can function as an activator or repressor depending on its binding partners, and by targeting negative regulators of cell cycle progression. Plays a critical role in early lymphocyte development, where it is essential to prevent apoptosis in lymphoid precursors, allowing them to survive in response to IL7 and undergo proper lineage commitment. Has been shown to bind to the promoters of adenovirus major late protein and cyclin D1 and activate transcription. Required for early embryonic development during gastrulation. Represses RB1 transcription; this repression can be blocked by interaction with ZBTB49 isoform 3/ZNF509S1. KEYWORDS: 3D-structure;Alternative splicing;Developmental protein;DNA-binding;Isopeptide bond;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Repeat;Transcription;Transcription regulation;Ubl conjugation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus " Q13114,"PROTEIN NAMES: TNF receptor-associated factor 3 (CD40 receptor-associated factor 1) (CRAF1) (CD40-binding protein) (CD40BP) (LMP1-associated protein 1) (LAP1) (RING-type E3 ubiquitin transferase TRAF3) PROTEIN FAMILY: TNF receptor-associated factor family, A subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the TNF receptor-associated factor family. A subfamily. FUNCTION: Cytoplasmic E3 ubiquitin ligase that regulates various signaling pathways, such as the NF-kappa-B, mitogen-activated protein kinase (MAPK) and interferon regulatory factor (IRF) pathways, and thus controls a lot of biological processes in both immune and non-immune cell types. In TLR and RLR signaling pathways, acts as an E3 ubiquitin ligase promoting the synthesis of 'Lys-63'-linked polyubiquitin chains on several substrates such as ASC that lead to the activation of the type I interferon response or the inflammasome. Following the activation of certain TLRs such as TLR4, acts as a negative NF-kappa-B regulator, possibly to avoid unregulated inflammatory response, and its degradation via 'Lys-48'-linked polyubiquitination is required for MAPK activation and production of inflammatory cytokines. Alternatively, when TLR4 orchestrates bacterial expulsion, TRAF3 undergoes 'Lys-33'-linked polyubiquitination and subsequently binds to RALGDS, mobilizing the exocyst complex to rapidly expel intracellular bacteria back for clearance. Acts also as a constitutive negative regulator of the alternative NF-kappa-B pathway, which controls B-cell survival and lymphoid organ development. Required for normal antibody isotype switching from IgM to IgG. Plays a role T-cell dependent immune responses. Down-regulates proteolytic processing of NFKB2, and thereby inhibits non-canonical activation of NF-kappa-B. Promotes ubiquitination and proteasomal degradation of MAP3K14. KEYWORDS: 3D-structure;Alternative splicing;Apoptosis;Coiled coil;Cytoplasm;Disease variant;Endosome;Host-virus interaction;Immunity;Isopeptide bond;Metal-binding;Mitochondrion;Phosphoprotein;Reference proteome;Repeat;Thioester bond;Transferase;Ubl conjugation;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cytoplasm Endosome Mitochondrion Note=Undergoes endocytosis together with TLR4 upon LPS signaling (By similarity). Co-localized to mitochondria with TRIM35. " Q13145,PROTEIN NAMES: BMP and activin membrane-bound inhibitor homolog (Non-metastatic gene A protein) (Putative transmembrane protein NMA) PROTEIN FAMILY: BAMBI family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the BAMBI family. FUNCTION: Negatively regulates TGF-beta signaling. KEYWORDS: Direct protein sequencing;Glycoprotein;Membrane;Reference proteome;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Single-pass type I membrane protein Q13163,"PROTEIN NAMES: Dual specificity mitogen-activated protein kinase kinase 5 (MAP kinase kinase 5) (MAPKK 5) (MAPK/ERK kinase 5) (MEK 5) PROTEIN FAMILY: Protein kinase superfamily, STE Ser/Thr protein kinase family, MAP kinase kinase subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily. FUNCTION: Acts as a scaffold for the formation of a ternary MAP3K2/MAP3K3-MAP3K5-MAPK7 signaling complex. Activation of this pathway appears to play a critical role in protecting cells from stress-induced apoptosis, neuronal survival and cardiac development and angiogenesis. As part of the MAPK/ERK signaling pathway, acts as a negative regulator of apoptosis in cardiomyocytes via promotion of STUB1/CHIP-mediated ubiquitination and degradation of ICER-type isoforms of CREM (By similarity). KEYWORDS: 3D-structure;Acetylation;Alternative splicing;ATP-binding;Kinase;Magnesium;Metal-binding;Nucleotide-binding;Phosphoprotein;Reference proteome;Serine/threonine-protein kinase;Transferase;Tyrosine-protein kinase MISCELLANEOUS: [Isoform C]: Incomplete sequence." Q13206,"PROTEIN NAMES: Probable ATP-dependent RNA helicase DDX10 (DEAD box protein 10) PROTEIN FAMILY: DEAD box helicase family, DDX10/DBP4 subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the DEAD box helicase family. DDX10/DBP4 subfamily. FUNCTION: Putative ATP-dependent RNA helicase. KEYWORDS: 3D-structure;Acetylation;ATP-binding;Helicase;Hydrolase;Isopeptide bond;Nucleotide-binding;Phosphoprotein;Reference proteome;RNA-binding;Ubl conjugation " Q13304,PROTEIN NAMES: Uracil nucleotide/cysteinyl leukotriene receptor (UDP/CysLT receptor) (G-protein coupled receptor 17) (P2Y-like receptor) (R12) PROTEIN FAMILY: G-protein coupled receptor 1 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the G-protein coupled receptor 1 family. FUNCTION: Dual specificity receptor for uracil nucleotides and cysteinyl leukotrienes (CysLTs). Signals through G(i) and inhibition of adenylyl cyclase. May mediate brain damage by nucleotides and CysLTs following ischemia. KEYWORDS: 3D-structure;Alternative splicing;Cell membrane;Disulfide bond;G-protein coupled receptor;Glycoprotein;Membrane;Receptor;Reference proteome;Transducer;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Q13309,"PROTEIN NAMES: S-phase kinase-associated protein 2 (Cyclin-A/CDK2-associated protein p45) (F-box protein Skp2) (F-box/LRR-repeat protein 1) (p45skp2) ORGANISM: Homo sapiens (Human) FUNCTION: Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition (By similarity). Degradation of CDKN1B/p27kip also requires CKS1 (By similarity). Recognizes target proteins ORC1, CDT1, RBL2, KMT2A/MLL1, CDK9, RAG2, NBN, FOXO1, UBP43, YTHDF2, and probably MYC, TOB1 and TAL1. Degradation of TAL1 also requires STUB1. Recognizes CDKN1A in association with CCNE1 or CCNE2 and CDK2. Promotes ubiquitination and destruction of CDH1 in a CK1-dependent manner, thereby regulating cell migration. Following phosphorylation in response to DNA damage, mediates 'Lys-63'-linked ubiquitination of NBN, promoting ATM recruitment to DNA damage sites and DNA repair via homologous recombination.; FUNCTION: Through the ubiquitin-mediated proteasomal degradation of hepatitis C virus non-structural protein 5A, has an antiviral activity towards that virus. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: 3D-structure;Acetylation;Alternative splicing;Antiviral defense;Cytoplasm;Direct protein sequencing;Host-virus interaction;Immunity;Innate immunity;Leucine-rich repeat;Nucleus;Phosphoprotein;Reference proteome;Repeat;Ubl conjugation;Ubl conjugation pathway SUBCELLULAR LOCATION: Cytoplasm Nucleus " Q13325,"PROTEIN NAMES: Interferon-induced protein with tetratricopeptide repeats 5 (IFIT-5) (Interferon-induced 58 kDa protein) (Retinoic acid- and interferon-inducible 58 kDa protein) (P58) PROTEIN FAMILY: IFIT family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the IFIT family. FUNCTION: Interferon-induced RNA-binding protein involved in the human innate immune response. Has a broad and adaptable RNA structure recognition important for RNA recognition specificity in antiviral defense. Binds precursor and processed tRNAs as well as poly-U-tailed tRNA fragments. Specifically binds single-stranded RNA bearing a 5'-triphosphate group (PPP-RNA), thereby acting as a sensor of viral single-stranded RNAs. Single-stranded PPP-RNAs, which lack 2'-O-methylation of the 5' cap and bear a 5'-triphosphate group instead, are specific from viruses, providing a molecular signature to distinguish between self and non-self mRNAs by the host during viral infection. Directly binds PPP-RNA in a non-sequence-specific manner. Also recognizes and selectively binds AT-rich dsDNA. Additionally, as a mediator in innate immunity, positively regulates IKK-NFKB signaling by sinergizing the recruitment of IKK to MAP3K7. KEYWORDS: 3D-structure;Alternative splicing;Antiviral defense;Cell membrane;Cell projection;Immunity;Innate immunity;Membrane;Reference proteome;Repeat;RNA-binding;TPR repeat;tRNA-binding SUBCELLULAR LOCATION: Cell projection, ruffle membrane Note=Colocalized with RIGI at cell surface ruffles. Localizes to actin-rich protrusions from the apical cell surface. " Q13330,"PROTEIN NAMES: Metastasis-associated protein MTA1 ORGANISM: Homo sapiens (Human) FUNCTION: Transcriptional coregulator which can act as both a transcriptional corepressor and coactivator. Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin. In the NuRD complex, regulates transcription of its targets by modifying the acetylation status of the target chromatin and cofactor accessibility to the target DNA. In conjunction with other components of NuRD, acts as a transcriptional corepressor of BRCA1, ESR1, TFF1 and CDKN1A. Acts as a transcriptional coactivator of BCAS3, and SUMO2, independent of the NuRD complex. Stimulates the expression of WNT1 by inhibiting the expression of its transcriptional corepressor SIX3 (By similarity). Regulates p53-dependent and -independent DNA repair processes following genotoxic stress. Regulates the stability and function of p53/TP53 by inhibiting its ubiquitination by COP1 and MDM2 thereby regulating the p53-dependent DNA repair. Plays a role in the regulation of the circadian clock and is essential for the generation and maintenance of circadian rhythms under constant light and for normal entrainment of behavior to light-dark (LD) cycles (By similarity). Positively regulates the CLOCK-BMAL1 heterodimer mediated transcriptional activation of its own transcription and the transcription of CRY1 (By similarity). Regulates deacetylation of BMAL1 by regulating SIRT1 expression, resulting in derepressing CRY1-mediated transcription repression (By similarity). With TFCP2L1, promotes establishment and maintenance of pluripotency in embryonic stem cells (ESCs) and inhibits endoderm differentiation (By similarity).; FUNCTION: [Isoform Short]: Binds to ESR1 and sequesters it in the cytoplasm and enhances its non-genomic responses. KEYWORDS: 3D-structure;Acetylation;Activator;Alternative splicing;Biological rhythms;Cytoplasm;Cytoskeleton;DNA-binding;Isopeptide bond;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Repressor;Transcription;Transcription regulation;Ubl conjugation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus.; SUBCELLULAR LOCATION: [Isoform Short]: Cytoplasm.; SUBCELLULAR LOCATION: [Isoform Long]: Nucleus Nucleus envelope Cytoplasm Cytoplasm, cytoskeleton Note=Associated with microtubules. Localization at the nuclear envelope is TPR-dependent. " Q13360,PROTEIN NAMES: Zinc finger protein 177 PROTEIN FAMILY: Krueppel C2H2-type zinc-finger protein family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein family. FUNCTION: May be involved in transcriptional regulation. KEYWORDS: Alternative splicing;DNA-binding;Metal-binding;Nucleus;Reference proteome;Repeat;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Q13361,"PROTEIN NAMES: Microfibrillar-associated protein 5 (MFAP-5) (MP25) (Microfibril-associated glycoprotein 2) (MAGP-2) PROTEIN FAMILY: MFAP family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the MFAP family. FUNCTION: May play a role in hematopoiesis. In the cardiovascular system, could regulate growth factors or participate in cell signaling in maintaining large vessel integrity (By similarity). Component of the elastin-associated microfibrils. KEYWORDS: Alternative splicing;Aortic aneurysm;Disease variant;Disulfide bond;Extracellular matrix;Glycoprotein;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix. " Q13371,"PROTEIN NAMES: Phosducin-like protein (PHLP) PROTEIN FAMILY: Phosducin family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the phosducin family. FUNCTION: Acts as a positive regulator of hedgehog signaling and regulates ciliary function.; FUNCTION: [Isoform 1]: Functions as a co-chaperone for CCT in the assembly of heterotrimeric G protein complexes, facilitates the assembly of both Gbeta-Ggamma and RGS-Gbeta5 heterodimers.; FUNCTION: [Isoform 2]: Acts as a negative regulator of heterotrimeric G proteins assembly by trapping the preloaded G beta subunits inside the CCT chaperonin. KEYWORDS: 3D-structure;Acetylation;Alternative splicing;Cell projection;Chaperone;Cilium biogenesis/degradation;Direct protein sequencing;Phosphoprotein;Reference proteome;Sensory transduction;Vision SUBCELLULAR LOCATION: Cell projection, cilium MISCELLANEOUS: [Isoform 2]: Expressed ubiquitously, highest levels are found in neural tissues amounting to 10% of total PDCL mRNA." Q13435,"PROTEIN NAMES: Splicing factor 3B subunit 2 (Pre-mRNA-splicing factor SF3b 145 kDa subunit) (SF3b145) (Spliceosome-associated protein 145) (SAP 145) ORGANISM: Homo sapiens (Human) FUNCTION: Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs. The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing. Within the 17S U2 SnRNP complex, SF3B2 is part of the SF3B subcomplex, which is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence in pre-mRNA. Sequence independent binding of SF3A and SF3B subcomplexes upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. Also acts as a component of the minor spliceosome, which is involved in the splicing of U12-type introns in pre-mRNAs. KEYWORDS: 3D-structure;Acetylation;Coiled coil;Direct protein sequencing;Disease variant;Host-virus interaction;Isopeptide bond;Methylation;mRNA processing;mRNA splicing;Nucleus;Phosphoprotein;Reference proteome;Spliceosome;Ubl conjugation SUBCELLULAR LOCATION: Nucleus Nucleus speckle " Q13454,PROTEIN NAMES: Tumor suppressor candidate 3 (Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit TUSC3) (Oligosaccharyl transferase subunit TUSC3) (Magnesium uptake/transporter TUSC3) (Protein N33) PROTEIN FAMILY: OST3/OST6 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the OST3/OST6 family. FUNCTION: Acts as accessory component of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. Involved in N-glycosylation of STT3B-dependent substrates. Specifically required for the glycosylation of a subset of acceptor sites that are near cysteine residues; in this function seems to act redundantly with MAGT1. In its oxidized form proposed to form transient mixed disulfides with a glycoprotein substrate to facilitate access of STT3B to the unmodified acceptor site. Has also oxidoreductase-independent functions in the STT3B-containing OST complex possibly involving substrate recognition.; FUNCTION: Magnesium transporter. PATHWAY: Protein modification; protein glycosylation. KEYWORDS: 3D-structure;Alternative splicing;Disulfide bond;Endoplasmic reticulum;Intellectual disability;Magnesium;Membrane;Reference proteome;Signal;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein Q13470,"PROTEIN NAMES: Non-receptor tyrosine-protein kinase TNK1 (CD38 negative kinase 1) PROTEIN FAMILY: Protein kinase superfamily, Tyr protein kinase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. FUNCTION: Involved in negative regulation of cell growth. Has tumor suppressor properties. Plays a negative regulatory role in the Ras-MAPK pathway. May function in signaling pathways utilized broadly during fetal development and more selectively in adult tissues and in cells of the lymphohematopoietic system. Could specifically be involved in phospholipid signal transduction. KEYWORDS: 3D-structure;Alternative splicing;ATP-binding;Cytoplasm;Kinase;Membrane;Nucleotide-binding;Phosphoprotein;Reference proteome;SH3 domain;Transferase;Tyrosine-protein kinase SUBCELLULAR LOCATION: Cytoplasm Membrane ; Peripheral membrane protein " Q13505,PROTEIN NAMES: Metaxin-1 (Mitochondrial outer membrane import complex protein 1) PROTEIN FAMILY: Metaxin family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the metaxin family. FUNCTION: Involved in transport of proteins into the mitochondrion. Essential for embryonic development (By similarity). KEYWORDS: Alternative splicing;Isopeptide bond;Membrane;Mitochondrion;Mitochondrion outer membrane;Protein transport;Reference proteome;Transmembrane;Transmembrane helix;Transport;Ubl conjugation SUBCELLULAR LOCATION: Membrane ; Single-pass type I membrane protein Mitochondrion outer membrane Q13530,"PROTEIN NAMES: Serine incorporator 3 (Tumor differentially expressed protein 1) PROTEIN FAMILY: TDE1 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the TDE1 family. FUNCTION: Restriction factor required to restrict infectivity of lentiviruses, such as HIV-1: acts by inhibiting an early step of viral infection. Impairs the penetration of the viral particle into the cytoplasm. KEYWORDS: 3D-structure;Alternative splicing;Antiviral defense;Cell membrane;Cytoplasm;Glycoprotein;Golgi apparatus;Host-virus interaction;Immunity;Innate immunity;Membrane;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Golgi apparatus membrane ; Multi-pass membrane protein.; SUBCELLULAR LOCATION: Cytoplasm, perinuclear region Note=(Microbial infection) Upon HIV-1 infection, it is redirected to perinuclear region following interaction with HIV-1 Nef, excluding it from virions particles, thereby preventing subsequent antiviral defense activity (Probable). " Q13546,"PROTEIN NAMES: Receptor-interacting serine/threonine-protein kinase 1 (Cell death protein RIP) (Receptor-interacting protein 1) (RIP-1) PROTEIN FAMILY: Protein kinase superfamily, TKL Ser/Thr protein kinase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. FUNCTION: Serine-threonine kinase which is a key regulator of TNF-mediated apoptosis, necroptosis and inflammatory pathways. Exhibits kinase activity-dependent functions that regulate cell death and kinase-independent scaffold functions regulating inflammatory signaling and cell survival. Has kinase-independent scaffold functions: upon binding of TNF to TNFR1, RIPK1 is recruited to the TNF-R1 signaling complex (TNF-RSC also known as complex I) where it acts as a scaffold protein promoting cell survival, in part, by activating the canonical NF-kappa-B pathway (By similarity). Kinase activity is essential to regulate necroptosis and apoptosis, two parallel forms of cell death: upon activation of its protein kinase activity, regulates assembly of two death-inducing complexes, namely complex IIa (RIPK1-FADD-CASP8), which drives apoptosis, and the complex IIb (RIPK1-RIPK3-MLKL), which drives necroptosis (By similarity). RIPK1 is required to limit CASP8-dependent TNFR1-induced apoptosis (By similarity). In normal conditions, RIPK1 acts as an inhibitor of RIPK3-dependent necroptosis, a process mediated by RIPK3 component of complex IIb, which catalyzes phosphorylation of MLKL upon induction by ZBP1. Inhibits RIPK3-mediated necroptosis via FADD-mediated recruitment of CASP8, which cleaves RIPK1 and limits TNF-induced necroptosis. Required to inhibit apoptosis and necroptosis during embryonic development: acts by preventing the interaction of TRADD with FADD thereby limiting aberrant activation of CASP8 (By similarity). In addition to apoptosis and necroptosis, also involved in inflammatory response by promoting transcriptional production of pro-inflammatory cytokines, such as interleukin-6 (IL6). Phosphorylates RIPK3: RIPK1 and RIPK3 undergo reciprocal auto- and trans-phosphorylation. Phosphorylates DAB2IP at 'Ser-728' in a TNF-alpha-dependent manner, and thereby activates the MAP3K5-JNK apoptotic cascade. Required for ZBP1-induced NF-kappa-B activation in response to DNA damage (By similarity). KEYWORDS: 3D-structure;Alternative splicing;Apoptosis;ATP-binding;Cell membrane;Cytoplasm;Disease variant;Glycoprotein;Host-virus interaction;Inflammatory response;Isopeptide bond;Kinase;Membrane;Necrosis;Nucleotide-binding;Phosphoprotein;Reference proteome;Serine/threonine-protein kinase;Transferase;Ubl conjugation SUBCELLULAR LOCATION: Cytoplasm Cell membrane " Q13585,"PROTEIN NAMES: Melatonin-related receptor (G protein-coupled receptor 50) (H9) [Cleaved into: C-terminal domain] PROTEIN FAMILY: G-protein coupled receptor 1 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the G-protein coupled receptor 1 family. FUNCTION: G protein-coupled receptor that plays a role in numerous physiological processes including regulation of energy metabolism, neurite outgrowth or cell migration. Promotes self-renewal and neuronal differentiation of neural progenitor cells through activation of the NOTCH and WNT/beta-catenin signaling pathways (By similarity). Modulates the KAT5-dependent glucocorticoid receptor signaling by modulating KAT5 subcellular compartmentalisation. Plays also a role in the activation TGFBR1 in the absence of TGFBR2 by interfering with FKBP1A binding to TGFBR1, leading to induction of both canonical and non-canonical SMAD signaling pathways resulting in inhibition of proliferation or promotion of migration.; FUNCTION: [C-terminal domain]: Upon cleavage by CAPN1, functions as a scaffold in the nucleus for interacting partners such as GTF2I to promote FOS promoter activation. KEYWORDS: Cell membrane;Disulfide bond;G-protein coupled receptor;Membrane;Nucleus;Receptor;Reference proteome;Synapse;Transducer;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein. Postsynaptic density.; SUBCELLULAR LOCATION: [C-terminal domain]: Nucleus " Q13642,"PROTEIN NAMES: Four and a half LIM domains protein 1 (FHL-1) (Skeletal muscle LIM-protein 1) (SLIM) (SLIM-1) ORGANISM: Homo sapiens (Human) FUNCTION: May have an involvement in muscle development or hypertrophy. KEYWORDS: 3D-structure;Acetylation;Alternative splicing;Cytoplasm;Developmental protein;Differentiation;Direct protein sequencing;Disease variant;Emery-Dreifuss muscular dystrophy;Isopeptide bond;LIM domain;Metal-binding;Nucleus;Reference proteome;Repeat;Ubl conjugation;Zinc;Zinc-finger SUBCELLULAR LOCATION: [Isoform 1]: Cytoplasm.; SUBCELLULAR LOCATION: [Isoform 3]: Cytoplasm. Nucleus.; SUBCELLULAR LOCATION: [Isoform 2]: Nucleus. Cytoplasm, cytosol. Note=Predominantly nuclear in myoblasts but is cytosolic in differentiated myotubes. " Q13724,"PROTEIN NAMES: Mannosyl-oligosaccharide glucosidase (Processing A-glucosidase I) PROTEIN FAMILY: Glycosyl hydrolase 63 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the glycosyl hydrolase 63 family. FUNCTION: In the context of N-glycan degradation, cleaves the distal alpha 1,2-linked glucose residue from the Glc(3)Man(9)GlcNAc(2) oligosaccharide precursor in a highly specific manner. PATHWAY: Glycan metabolism; N-glycan degradation. KEYWORDS: Alternative splicing;Disease variant;Endoplasmic reticulum;Glycoprotein;Glycosidase;Hydrolase;Membrane;Reference proteome;Signal-anchor;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Single-pass type II membrane protein " Q13825,"PROTEIN NAMES: Methylglutaconyl-CoA hydratase, mitochondrial (3-MG-CoA hydratase) (AU-specific RNA-binding enoyl-CoA hydratase) (AU-binding protein/enoyl-CoA hydratase) (Itaconyl-CoA hydratase) PROTEIN FAMILY: Enoyl-CoA hydratase/isomerase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the enoyl-CoA hydratase/isomerase family. FUNCTION: Catalyzes the fifth step in the leucine degradation pathway, the reversible hydration of 3-methylglutaconyl-CoA (3-MG-CoA) to 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA). Can catalyze the reverse reaction but at a much lower rate in vitro. HMG-CoA is then quickly degraded by another enzyme (such as HMG-CoA lyase) to give acetyl-CoA and acetoacetate. Uses other substrates such as (2E)-glutaconyl-CoA efficiently in vitro, and to a lesser extent 3-methylcrotonyl-CoA (3-methyl-(2E)-butenoyl-CoA), crotonyl-CoA ((2E)-butenoyl-CoA) and 3-hydroxybutanoyl-CoA (the missing carboxylate reduces affinity to the active site). Originally it was identified as an RNA-binding protein as it binds to AU-rich elements (AREs) in vitro. AREs direct rapid RNA degradation and mRNA deadenylation. Might have itaconyl-CoA hydratase activity, converting itaconyl-CoA into citramalyl-CoA in the C5-dicarboxylate catabolism pathway. The C5-dicarboxylate catabolism pathway is required to detoxify itaconate, an antimicrobial metabolite and immunomodulator produced by macrophages during certain infections, that can act as a vitamin B12-poisoning metabolite. PATHWAY: Amino-acid degradation; L-leucine degradation; (S)-3-hydroxy-3-methylglutaryl-CoA from 3-isovaleryl-CoA: step 3/3. KEYWORDS: 3D-structure;Acetylation;Alternative splicing;Branched-chain amino acid catabolism;Direct protein sequencing;Disease variant;Lyase;Mitochondrion;Reference proteome;RNA-binding;Transit peptide SUBCELLULAR LOCATION: Mitochondrion " Q13887,PROTEIN NAMES: Krueppel-like factor 5 (Basic transcription element-binding protein 2) (BTE-binding protein 2) (Colon krueppel-like factor) (GC-box-binding protein 2) (Intestinal-enriched krueppel-like factor) (Transcription factor BTEB2) PROTEIN FAMILY: Krueppel C2H2-type zinc-finger protein family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein family. FUNCTION: Transcription factor that binds to GC box promoter elements. Activates the transcription of these genes. KEYWORDS: 3D-structure;Activator;Alternative splicing;DNA-binding;Isopeptide bond;Metal-binding;Nucleus;Reference proteome;Repeat;Transcription;Transcription regulation;Ubl conjugation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Q13938,PROTEIN NAMES: Calcyphosin (Calcyphosine) ORGANISM: Homo sapiens (Human) FUNCTION: Calcium-binding protein. May play a role in cellular signaling events (Potential). KEYWORDS: 3D-structure;Alternative splicing;Calcium;Cytoplasm;Metal-binding;Phosphoprotein;Reference proteome;Repeat SUBCELLULAR LOCATION: Cytoplasm. Q13946,"PROTEIN NAMES: High affinity 3',5'-cyclic-AMP phosphodiesterase 7A (HCP1) (TM22) (cAMP-specific phosphodiesterase 7A) PROTEIN FAMILY: Cyclic nucleotide phosphodiesterase family, PDE7 subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the cyclic nucleotide phosphodiesterase family. PDE7 subfamily. FUNCTION: Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May have a role in muscle signal transduction. PATHWAY: Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1. KEYWORDS: 3D-structure;Alternative splicing;cAMP;Cytoplasm;Hydrolase;Metal-binding;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: [Isoform PDE7A1]: Cytoplasm, cytosol Note=PDE7A1 (57 kDa) is located mostly to soluble cellular fractions.; SUBCELLULAR LOCATION: [Isoform PDE7A2]: Cytoplasm Note=PDE7A2 (50 kDa) is located to particulate cellular fractions. " Q14142,"PROTEIN NAMES: Tripartite motif-containing protein 14 PROTEIN FAMILY: TRIM/RBCC family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the TRIM/RBCC family. FUNCTION: Plays an essential role in the innate immune defense against viruses and bacteria. Promotes the 'Lys-48'-linked ubiquitination and subsequent degradation of hepatitis C virus NS5A leading to the inhibition of viral replication. Plays also a role in the inhibition of ebolavirus infection by enhancing IFN-beta and NF-kappa-B activation after binding to the viral protein NP. Facilitates the type I IFN response by interacting with MAVS at the outer mitochondria membrane and thereby recruiting NF-kappa-B essential modulator IKBKG/NEMO to the MAVS signalosome, leading to the activation of both the IFN regulatory factor 3/IRF3 and NF-kappa-B pathways. Positively regulates the CGAS-induced type I interferon signaling pathway by stabilizing CGAS and inhibiting its autophagic degradation. Acts as a scaffold between TBK1 and STAT3 to promote phosphorylation of STAT3 and resolve interferon-stimulated gene (ISG) expression. Inhibits the transcriptional activity of SPI1 in a dose-dependent manner (By similarity). Inhibits also OPTN-mediated selective autophagic degradation of KDM4D and thereby negatively regulates H3K9me2 and H3K9me3. Mechanistically, recruits USP14 to remove the 'Lys-63'-linked ubiquitination of KDM4D, preventing its recognition by OPTN and subsequent degradation. KEYWORDS: 3D-structure;Alternative splicing;Coiled coil;Cytoplasmic vesicle;Immunity;Innate immunity;Membrane;Metal-binding;Mitochondrion;Mitochondrion outer membrane;Nucleus;Reference proteome;Ubl conjugation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Mitochondrion outer membrane Cytoplasmic vesicle, phagosome Nucleus " Q14165,PROTEIN NAMES: Malectin PROTEIN FAMILY: Malectin family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the malectin family. FUNCTION: Carbohydrate-binding protein with a strong ligand preference for Glc2-N-glycan. May play a role in the early steps of protein N-glycosylation (By similarity). KEYWORDS: 3D-structure;Carbohydrate metabolism;Direct protein sequencing;Endoplasmic reticulum;Glycoprotein;Membrane;Reference proteome;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Single-pass type I membrane protein Q14190,PROTEIN NAMES: Single-minded homolog 2 (Class E basic helix-loop-helix protein 15) (bHLHe15) ORGANISM: Homo sapiens (Human) FUNCTION: Transcription factor that may be a master gene of CNS development in cooperation with Arnt. It may have pleiotropic effects in the tissues expressed during development. KEYWORDS: Alternative splicing;Developmental protein;Differentiation;DNA-binding;Neurogenesis;Nucleus;Reference proteome;Repeat;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus Q14296,"PROTEIN NAMES: Fas-activated serine/threonine kinase (FAST kinase) PROTEIN FAMILY: FAST protein kinase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the FAST protein kinase family. FUNCTION: Phosphorylates the splicing regulator TIA1, thereby promoting the inclusion of FAS exon 6, which leads to an mRNA encoding a pro-apoptotic form of the receptor.; FUNCTION: [Isoform 4]: Required for the biogenesis of some mitochondrial-encoded mRNAs, specifically stabilizes ND6 (NADH dehydrogenase complex subunit 6) mRNA, and regulates its levels. KEYWORDS: Alternative initiation;Alternative splicing;Apoptosis;ATP-binding;Kinase;Mitochondrion;Nucleotide-binding;Phosphoprotein;Reference proteome;RNA-binding;Serine/threonine-protein kinase;Transferase SUBCELLULAR LOCATION: [Isoform 4]: Mitochondrion matrix Note=Colocalizes with mitochondrial RNA granules. " Q14406,PROTEIN NAMES: Chorionic somatomammotropin hormone-like 1 (Chorionic somatomammotropin-like) (Lactogen-like) PROTEIN FAMILY: Somatotropin/prolactin family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the somatotropin/prolactin family. FUNCTION: May be a novel gestational hormone required to compensate for absence of other members of the GH/CS cluster during gestation. KEYWORDS: Alternative splicing;Disulfide bond;Hormone;Metal-binding;Reference proteome;Secreted;Signal;Zinc SUBCELLULAR LOCATION: Secreted Q14444,"PROTEIN NAMES: Caprin-1 (Cell cycle-associated protein 1) (Cytoplasmic activation- and proliferation-associated protein 1) (GPI-anchored membrane protein 1) (GPI-anchored protein p137) (GPI-p137) (p137GPI) (Membrane component chromosome 11 surface marker 1) (RNA granule protein 105) PROTEIN FAMILY: Caprin family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the caprin family. FUNCTION: mRNA-binding protein that acts as a regulator of mRNAs transport, translation and/or stability, and which is involved in synaptic plasticity in neurons and cell proliferation and migration in multiple cell types. Plays an essential role in cytoplasmic stress granule formation. Acts as an mRNA regulator by mediating formation of some phase-separated membraneless compartment: undergoes liquid-liquid phase separation upon binding to target mRNAs, leading to assemble mRNAs into cytoplasmic ribonucleoprotein granules that concentrate mRNAs with associated regulatory factors. Undergoes liquid-liquid phase separation following phosphorylation and interaction with FMR1, promoting formation of cytoplasmic ribonucleoprotein granules that concentrate mRNAs with factors that inhibit translation and mediate deadenylation of target mRNAs. In these cytoplasmic ribonucleoprotein granules, CAPRIN1 mediates recruitment of CNOT7 deadenylase, leading to mRNA deadenylation and degradation. Binds directly and selectively to MYC and CCND2 mRNAs. In neuronal cells, directly binds to several mRNAs associated with RNA granules, including BDNF, CAMK2A, CREB1, MAP2, NTRK2 mRNAs, as well as to GRIN1 and KPNB1 mRNAs, but not to rRNAs. KEYWORDS: 3D-structure;Acetylation;Alternative splicing;ATP-binding;Cell projection;Coiled coil;Cytoplasm;Differentiation;Direct protein sequencing;Glycoprotein;Methylation;Nucleotide-binding;Phosphoprotein;Protein synthesis inhibitor;Reference proteome;RNA-binding SUBCELLULAR LOCATION: Cytoplasm, Cytoplasmic ribonucleoprotein granule Cytoplasm, cytosol Cell projection, dendrite Cell projection, lamellipodium Note=Mediates formation and localizes to cytoplasmic ribonucleoprotein membraneless compartments. Associated with RNA granules. At the leading edge of migrating fibroblasts, colocalizes with DDX3X.; SUBCELLULAR LOCATION: Cytoplasm, cytosol. Note=(Microbial infection) In case of reovirus infection, associates with the outer peripheries of viral factories in a G3BP1 dependent manner. " Q14515,"PROTEIN NAMES: SPARC-like protein 1 (High endothelial venule protein) (Hevin) (MAST 9) PROTEIN FAMILY: SPARC family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the SPARC family. KEYWORDS: 3D-structure;Alternative splicing;Calcium;Disulfide bond;Extracellular matrix;Glycoprotein;Metal-binding;Phosphoprotein;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix " Q14526,"PROTEIN NAMES: Hypermethylated in cancer 1 protein (Hic-1) (Zinc finger and BTB domain-containing protein 29) PROTEIN FAMILY: Krueppel C2H2-type zinc-finger protein family, Hic subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein family. Hic subfamily. FUNCTION: Transcriptional repressor. Recognizes and binds to the consensus sequence '5-[CG]NG[CG]GGGCA[CA]CC-3'. May act as a tumor suppressor. Involved in development of head, face, limbs and ventral body wall (By similarity). Involved in down-regulation of SIRT1 and thereby is involved in regulation of p53/TP53-dependent apoptotic DNA-damage responses. The specific target gene promoter association seems to be depend on corepressors, such as CTBP1 or CTBP2 and MTA1. In cooperation with MTA1 (indicative for an association with the NuRD complex) represses transcription from CCND1/cyclin-D1 and CDKN1C/p57Kip2 specifically in quiescent cells. Involved in regulation of the Wnt signaling pathway probably by association with TCF7L2 and preventing TCF7L2 and CTNNB1 association with promoters of TCF-responsive genes. Seems to repress transcription from E2F1 and ATOH1 which involves ARID1A, indicative for the participation of a distinct SWI/SNF-type chromatin-remodeling complex. Probably represses transcription of ACKR3, FGFBP1 and EFNA1. KEYWORDS: Acetylation;Alternative splicing;Developmental protein;DNA-binding;Isopeptide bond;Metal-binding;Methylation;Nucleus;Phosphoprotein;Reference proteome;Repeat;Repressor;Transcription;Transcription regulation;Tumor suppressor;Ubl conjugation;Wnt signaling pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: The HIC1 gene is frequently found epigenetically silenced or deleted in different types of solid tumors." Q14587,PROTEIN NAMES: Zinc finger protein 268 (Zinc finger protein HZF3) PROTEIN FAMILY: Krueppel C2H2-type zinc-finger protein family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein family. FUNCTION: [Isoform 1]: Acts as a transcriptional repressor. Inhibits erythroid differentiation and tumor cell proliferation. Plays a role during ovarian cancer development and progression.; FUNCTION: [Isoform 2]: Contributes to cervical carcinogenesis in part through the TNF-alpha-induced NF-kappa-B signaling pathway by interacting with the I-kappa-B-kinase (IKK) core complex. KEYWORDS: 3D-structure;Alternative splicing;Cytoplasm;Differentiation;DNA-binding;Metal-binding;Nucleus;Reference proteome;Repeat;Repressor;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: [Isoform 1]: Nucleus.; SUBCELLULAR LOCATION: [Isoform 2]: Nucleus Cytoplasm Q14656,PROTEIN NAMES: Transmembrane protein 187 (Protein ITBA1) ORGANISM: Homo sapiens (Human) KEYWORDS: Membrane;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Multi-pass membrane protein Q14684,"PROTEIN NAMES: Ribosomal RNA processing protein 1 homolog B (RRP1-like protein B) PROTEIN FAMILY: RRP1 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the RRP1 family. FUNCTION: Positively regulates DNA damage-induced apoptosis by acting as a transcriptional coactivator of proapoptotic target genes of the transcriptional activator E2F1. Likely to play a role in ribosome biogenesis by targeting serine/threonine protein phosphatase PP1 to the nucleolus. Involved in regulation of mRNA splicing (By similarity). Inhibits SIPA1 GTPase activity (By similarity). Involved in regulating expression of extracellular matrix genes (By similarity). Associates with chromatin and may play a role in modulating chromatin structure.; FUNCTION: (Microbial infection) Following influenza A virus (IAV) infection, promotes viral mRNA transcription by facilitating the binding of IAV RNA-directed RNA polymerase to capped mRNA. KEYWORDS: 3D-structure;Acetylation;Activator;Alternative splicing;Apoptosis;Chromosome;Citrullination;Host-virus interaction;mRNA processing;mRNA splicing;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus, nucleolus Nucleus, nucleoplasm Chromosome Note=Predominantly located in the nucleolus with a small amount found in the nucleoplasm. Associates with the perichromatin region during metaphase and with cytoplasmic foci during telophase before reaccumulation in the nucleolus during G2. Associates with heterochromatin and euchromatin.; SUBCELLULAR LOCATION: Nucleus, nucleoplasm Note=(Microbial infection) Following infection by influenza A virus, partially translocates from the nucleolus to the nucleoplasm. " Q14722,"PROTEIN NAMES: Voltage-gated potassium channel subunit beta-1 (K(+) channel subunit beta-1) (Kv-beta-1) PROTEIN FAMILY: Shaker potassium channel beta subunit family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the shaker potassium channel beta subunit family. FUNCTION: Cytoplasmic potassium channel subunit that modulates the characteristics of the channel-forming alpha-subunits. Modulates action potentials via its effect on the pore-forming alpha subunits (By similarity). Promotes expression of the pore-forming alpha subunits at the cell membrane, and thereby increases channel activity (By similarity). Mediates closure of delayed rectifier potassium channels by physically obstructing the pore via its N-terminal domain and increases the speed of channel closure for other family members. Promotes the closure of KCNA1, KCNA2 and KCNA5 channels. Accelerates KCNA4 channel closure. Accelerates the closure of heteromeric channels formed by KCNA1 and KCNA4. Accelerates the closure of heteromeric channels formed by KCNA2, KCNA5 and KCNA6 (By similarity). Isoform KvB1.2 has no effect on KCNA1, KCNA2 or KCNB1. Enhances KCNB1 and KCNB2 channel activity (By similarity). Binds NADPH; this is required for efficient down-regulation of potassium channel activity. Has NADPH-dependent aldoketoreductase activity (By similarity). Oxidation of the bound NADPH strongly decreases N-type inactivation of potassium channel activity (By similarity). KEYWORDS: Alternative splicing;Cell membrane;Cytoplasm;Ion channel;Ion transport;Membrane;NADP;Oxidoreductase;Potassium;Potassium transport;Reference proteome;Transport;Voltage-gated channel SUBCELLULAR LOCATION: Cytoplasm Membrane ; Peripheral membrane protein ; Cytoplasmic side Cell membrane ; Peripheral membrane protein ; Cytoplasmic side Note=Recruited to the cytoplasmic side of the cell membrane via its interaction with pore-forming potassium channel alpha subunits. " Q14764,"PROTEIN NAMES: Major vault protein (MVP) (Lung resistance-related protein) ORGANISM: Homo sapiens (Human) FUNCTION: Required for normal vault structure. Vaults are multi-subunit structures that may act as scaffolds for proteins involved in signal transduction. Vaults may also play a role in nucleo-cytoplasmic transport. Down-regulates IFNG-mediated STAT1 signaling and subsequent activation of JAK. Down-regulates SRC activity and signaling through MAP kinases. KEYWORDS: 3D-structure;Acetylation;Cytoplasm;Direct protein sequencing;Isopeptide bond;mRNA transport;Nuclear pore complex;Nucleus;Phosphoprotein;Protein transport;Reference proteome;Repeat;Ribonucleoprotein;Translocation;Transport;Ubl conjugation SUBCELLULAR LOCATION: Cytoplasm Nucleus, nuclear pore complex Cytoplasm, perinuclear region Note=5% found in the nuclear pore complex. Translocates from the nucleus to the cytoplasm upon EGF treatment. " Q14807,"PROTEIN NAMES: Kinesin-like protein KIF22 (Kinesin-like DNA-binding protein) (Kinesin-like protein 4) PROTEIN FAMILY: TRAFAC class myosin-kinesin ATPase superfamily, Kinesin family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. FUNCTION: Kinesin family member that is involved in spindle formation and the movements of chromosomes during mitosis and meiosis. Binds to microtubules and to DNA (By similarity). Plays a role in congression of laterally attached chromosomes in NDC80-depleted cells. KEYWORDS: 3D-structure;Alternative splicing;ATP-binding;Coiled coil;Cytoplasm;Cytoskeleton;Disease variant;DNA-binding;Dwarfism;Isopeptide bond;Microtubule;Motor protein;Nucleotide-binding;Nucleus;Phosphoprotein;Reference proteome;Ubl conjugation SUBCELLULAR LOCATION: Nucleus Cytoplasm, cytoskeleton " Q14995,"PROTEIN NAMES: Nuclear receptor subfamily 1 group D member 2 (Orphan nuclear hormone receptor BD73) (Rev-erb alpha-related receptor) (RVR) (Rev-erb-beta) (V-erbA-related protein 1-related) (EAR-1R) PROTEIN FAMILY: Nuclear hormone receptor family, NR1 subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the nuclear hormone receptor family. NR1 subfamily. FUNCTION: Transcriptional repressor which coordinates circadian rhythm and metabolic pathways in a heme-dependent manner. Integral component of the complex transcription machinery that governs circadian rhythmicity and forms a critical negative limb of the circadian clock by directly repressing the expression of core clock components BMAL1 and CLOCK. Also regulates genes involved in metabolic functions, including lipid metabolism and the inflammatory response. Acts as a receptor for heme which stimulates its interaction with the NCOR1/HDAC3 corepressor complex, enhancing transcriptional repression. Recognizes two classes of DNA response elements within the promoter of its target genes and can bind to DNA as either monomers or homodimers, depending on the nature of the response element. Binds as a monomer to a response element composed of the consensus half-site motif 5'-[A/G]GGTCA-3' preceded by an A/T-rich 5' sequence (RevRE), or as a homodimer to a direct repeat of the core motif spaced by two nuclegotides (RevDR-2). Acts as a potent competitive repressor of ROR alpha (RORA) function and also negatively regulates the expression of NR1D1. Regulates lipid and energy homeostasis in the skeletal muscle via repression of genes involved in lipid metabolism and myogenesis including: CD36, FABP3, FABP4, UCP3, SCD1 and MSTN. Regulates hepatic lipid metabolism via the repression of APOC3. Represses gene expression at a distance in macrophages by inhibiting the transcription of enhancer-derived RNAs (eRNAs). In addition to its activity as a repressor, can also act as a transcriptional activator. Acts as a transcriptional activator of the sterol regulatory element-binding protein 1 (SREBF1) and the inflammatory mediator interleukin-6 (IL6) in the skeletal muscle (By similarity). Plays a role in the regulation of circadian sleep/wake cycle; essential for maintaining wakefulness during the dark phase or active period (By similarity). Key regulator of skeletal muscle mitochondrial function; negatively regulates the skeletal muscle expression of core clock genes and genes involved in mitochondrial biogenesis, fatty acid beta-oxidation and lipid metabolism (By similarity). May play a role in the circadian control of neutrophilic inflammation in the lung (By similarity). KEYWORDS: 3D-structure;Acetylation;Activator;Biological rhythms;Cytoplasm;Disulfide bond;DNA-binding;Heme;Iron;Metal-binding;Nucleus;Phosphoprotein;Receptor;Reference proteome;Repressor;Transcription;Transcription regulation;Ubl conjugation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Cytoplasm Note=Phosphorylation by CSNK1E enhances its cytoplasmic localization. " Q149S1,"PROTEIN NAMES: Tektin-4 (Testicular microtubules-related protein 4) PROTEIN FAMILY: Tektin family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the tektin family. FUNCTION: Microtubule inner protein (MIP) part of the dynein-decorated doublet microtubules (DMTs) in cilia and flagellar axoneme. Forms filamentous polymers in the walls of ciliary and flagellar microtubules (By similarity). Contributes to normal sperm motility. KEYWORDS: 3D-structure;Cell projection;Cilium;Cilium biogenesis/degradation;Coiled coil;Cytoplasm;Cytoskeleton;Flagellum;Reference proteome SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, cilium axoneme Cell projection, cilium, flagellum Note=Found in the abaxial (convex) surface of outer dense fibers in sperm flagella. " Q14CX7,"PROTEIN NAMES: N-alpha-acetyltransferase 25, NatB auxiliary subunit (Mitochondrial distribution and morphology protein 20) (N-terminal acetyltransferase B complex subunit MDM20) (NatB complex subunit MDM20) (N-terminal acetyltransferase B complex subunit NAA25) (p120) PROTEIN FAMILY: MDM20/NAA25 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the MDM20/NAA25 family. FUNCTION: Non-catalytic subunit of the NatB complex which catalyzes acetylation of the N-terminal methionine residues of peptides beginning with Met-Asp, Met-Glu, Met-Asn and Met-Gln. May play a role in normal cell-cycle progression. KEYWORDS: 3D-structure;Alternative splicing;Cytoplasm;Reference proteome;Repeat;TPR repeat SUBCELLULAR LOCATION: Cytoplasm " Q14CZ7,"PROTEIN NAMES: FAST kinase domain-containing protein 3, mitochondrial PROTEIN FAMILY: FAST kinase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the FAST kinase family. FUNCTION: Required for normal mitochondrial respiration. Increases steady-state levels and half-lives of a subset of mature mitochondrial mRNAs MT-ND2, MT-ND3, MT-CYTB, MT-CO2, and MT-ATP8/6. Promotes MT-CO1 mRNA translation and increases mitochondrial complex IV assembly and activity. KEYWORDS: Mitochondrion;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Mitochondrion " Q14D33,PROTEIN NAMES: Receptor-transporting protein 5 (3CxxC-type zinc finger protein 5) (CXXC-type zinc finger protein 11) ORGANISM: Homo sapiens (Human) KEYWORDS: Membrane;Metal-binding;Reference proteome;Transmembrane;Transmembrane helix;Zinc;Zinc-finger SUBCELLULAR LOCATION: Membrane ; Single-pass membrane protein Q15003,"PROTEIN NAMES: Condensin complex subunit 2 (Barren homolog protein 1) (Chromosome-associated protein H) (hCAP-H) (Non-SMC condensin I complex subunit H) (XCAP-H homolog) PROTEIN FAMILY: CND2 (condensin subunit 2) family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the CND2 (condensin subunit 2) family. FUNCTION: Regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases. Early in neurogenesis, may play an essential role to ensure accurate mitotic chromosome condensation in neuron stem cells, ultimately affecting neuron pool and cortex size. KEYWORDS: 3D-structure;Acetylation;Alternative splicing;Cell cycle;Cell division;Chromosome;Cytoplasm;Disease variant;DNA condensation;Isopeptide bond;Mitosis;Nucleus;Phosphoprotein;Primary microcephaly;Reference proteome;Ubl conjugation SUBCELLULAR LOCATION: Nucleus Cytoplasm Chromosome Note=In interphase cells, the majority of the condensin complex is found in the cytoplasm, while a minority of the complex is associated with chromatin. A subpopulation of the complex however remains associated with chromosome foci in interphase cells. During mitosis, most of the condensin complex is associated with the chromatin. At the onset of prophase, the regulatory subunits of the complex are phosphorylated by CDK1, leading to condensin's association with chromosome arms and to chromosome condensation. Dissociation from chromosomes is observed in late telophase. " Q15005,PROTEIN NAMES: Signal peptidase complex subunit 2 (Microsomal signal peptidase 25 kDa subunit) (SPase 25 kDa subunit) PROTEIN FAMILY: SPCS2 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the SPCS2 family. FUNCTION: Component of the signal peptidase complex (SPC) which catalyzes the cleavage of N-terminal signal sequences from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum. Enhances the enzymatic activity of SPC and facilitates the interactions between different components of the translocation site (By similarity). KEYWORDS: 3D-structure;Acetylation;Direct protein sequencing;Endoplasmic reticulum;Membrane;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein Q15008,"PROTEIN NAMES: 26S proteasome non-ATPase regulatory subunit 6 (26S proteasome regulatory subunit RPN7) (26S proteasome regulatory subunit S10) (Breast cancer-associated protein SGA-113M) (Phosphonoformate immuno-associated protein 4) (Proteasome regulatory particle subunit p44S10) (p42A) PROTEIN FAMILY: Proteasome subunit S10 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the proteasome subunit S10 family. FUNCTION: Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. KEYWORDS: 3D-structure;Alternative splicing;Proteasome;Reference proteome " Q15024,"PROTEIN NAMES: Exosome complex component RRP42 (Exosome component 7) (Ribosomal RNA-processing protein 42) (p8) PROTEIN FAMILY: RNase PH family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the RNase PH family. FUNCTION: Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. KEYWORDS: 3D-structure;Acetylation;Cytoplasm;Direct protein sequencing;Exosome;Nucleus;Phosphoprotein;Reference proteome;RNA-binding;rRNA processing SUBCELLULAR LOCATION: Nucleus, nucleolus Cytoplasm Nucleus " Q15042,"PROTEIN NAMES: Rab3 GTPase-activating protein catalytic subunit (RAB3 GTPase-activating protein 130 kDa subunit) (Rab3-GAP p130) (Rab3-GAP) PROTEIN FAMILY: Rab3-GAP catalytic subunit family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the Rab3-GAP catalytic subunit family. FUNCTION: Catalytic subunit of the Rab3 GTPase-activating (Rab3GAP) complex composed of RAB3GAP1 and RAB3GAP2, which has GTPase-activating protein (GAP) activity towards various Rab3 subfamily members (RAB3A, RAB3B, RAB3C and RAB3D), RAB5A and RAB43, and guanine nucleotide exchange factor (GEF) activity towards RAB18. As part of the Rab3GAP complex, acts as a GAP for Rab3 proteins by converting active RAB3-GTP to the inactive form RAB3-GDP. Rab3 proteins are involved in regulated exocytosis of neurotransmitters and hormones. The Rab3GAP complex, acts as a GEF for RAB18 by promoting the conversion of inactive RAB18-GDP to the active form RAB18-GTP. Required for recruiting and activating RAB18 at the endoplasmic reticulum (ER) membrane where it maintains proper ER structure. Required for normal eye and brain development. May participate in neurodevelopmental processes such as proliferation, migration and differentiation before synapse formation, and non-synaptic vesicular release of neurotransmitters. KEYWORDS: Alternative splicing;Cataract;Cytoplasm;Disease variant;Endoplasmic reticulum;GTPase activation;Intellectual disability;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Endoplasmic reticulum Note=In neurons, it is enriched in the synaptic soluble fraction. " Q15061,"PROTEIN NAMES: WD repeat-containing protein 43 (U3 small nucleolar RNA-associated protein 5 homolog) PROTEIN FAMILY: UTP5 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the UTP5 family. FUNCTION: Ribosome biogenesis factor that coordinates hyperactive transcription and ribogenesis. Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome. Involved in nucleolar processing of pre-18S ribosomal RNA. Required for optimal pre-ribosomal RNA transcription by RNA polymerase I. Essential for stem cell pluripotency and embryonic development. In the nucleoplasm, recruited by promoter-associated/nascent transcripts and transcription to active promoters where it facilitates releases of elongation factor P-TEFb and paused RNA polymerase II to allow transcription elongation and maintain high-level expression of its targets genes (By similarity). KEYWORDS: 3D-structure;Isopeptide bond;Nucleus;Phosphoprotein;Reference proteome;Repeat;Ribosome biogenesis;RNA-binding;rRNA processing;Transcription;Transcription regulation;Ubl conjugation;WD repeat SUBCELLULAR LOCATION: Nucleus, nucleolus Nucleus, nucleolus fibrillar center Nucleus, nucleoplasm " Q15124,"PROTEIN NAMES: Phosphoglucomutase-like protein 5 (Aciculin) (Phosphoglucomutase-related protein) (PGM-RP) PROTEIN FAMILY: Phosphohexose mutase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the phosphohexose mutase family. FUNCTION: Component of adherens-type cell-cell and cell-matrix junctions. Has no phosphoglucomutase activity in vitro. KEYWORDS: 3D-structure;Alternative splicing;Cell adhesion;Cell junction;Cell membrane;Cytoplasm;Cytoskeleton;Direct protein sequencing;Membrane;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cell junction, adherens junction Cytoplasm, cytoskeleton Cell membrane, sarcolemma Note=Concentrated in focal contacts at the ends of actin bundles, and associated with actin filaments. " Q15223,"PROTEIN NAMES: Nectin-1 (Herpes virus entry mediator C) (Herpesvirus entry mediator C) (HveC) (Herpesvirus Ig-like receptor) (HIgR) (Nectin cell adhesion molecule 1) (Poliovirus receptor-related protein 1) (CD antigen CD111) PROTEIN FAMILY: Nectin family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the nectin family. FUNCTION: Promotes cell-cell contacts by forming homophilic or heterophilic trans-dimers. Heterophilic interactions have been detected between NECTIN1 and NECTIN3 and between NECTIN1 and NECTIN4. Has some neurite outgrowth-promoting activity.; FUNCTION: (Microbial infection) Acts as a receptor for herpes simplex virus 1/HHV-1, herpes simplex virus 2/HHV-2, and pseudorabies virus/PRV. KEYWORDS: 3D-structure;Alternative splicing;Cell adhesion;Cell membrane;Cell projection;Disulfide bond;Ectodermal dysplasia;Glycoprotein;Host cell receptor for virus entry;Host-virus interaction;Immunoglobulin domain;Membrane;Phosphoprotein;Receptor;Reference proteome;Repeat;Secreted;Signal;Synapse;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: [Isoform Alpha]: Cell membrane; Single-pass type I membrane protein. Presynaptic cell membrane.; SUBCELLULAR LOCATION: [Isoform Delta]: Cell membrane; Single-pass type I membrane protein.; SUBCELLULAR LOCATION: [Isoform Gamma]: Secreted. " Q15269,"PROTEIN NAMES: Periodic tryptophan protein 2 homolog PROTEIN FAMILY: WD repeat PWP2 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the WD repeat PWP2 family. FUNCTION: Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome. KEYWORDS: 3D-structure;Nucleus;Phosphoprotein;Reference proteome;Repeat;WD repeat SUBCELLULAR LOCATION: Nucleus, nucleolus " Q15326,"PROTEIN NAMES: Zinc finger MYND domain-containing protein 11 (Adenovirus 5 E1A-binding protein) (Bone morphogenetic protein receptor-associated molecule 1) (Protein BS69) ORGANISM: Homo sapiens (Human) FUNCTION: Chromatin reader that specifically recognizes and binds histone H3.3 trimethylated at 'Lys-36' (H3.3K36me3) and regulates RNA polymerase II elongation. Does not bind other histone H3 subtypes (H3.1 or H3.2) (By similarity). Colocalizes with highly expressed genes and functions as a transcription corepressor by modulating RNA polymerase II at the elongation stage. Binds non-specifically to dsDNA. Acts as a tumor-suppressor by repressing a transcriptional program essential for tumor cell growth.; FUNCTION: (Microbial infection) Inhibits Epstein-Barr virus EBNA2-mediated transcriptional activation and host cell proliferation, through direct interaction. KEYWORDS: 3D-structure;Alternative splicing;Bromodomain;Cell cycle;Chromatin regulator;Chromosomal rearrangement;Chromosome;DNA-binding;Host-virus interaction;Intellectual disability;Isopeptide bond;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Repressor;Transcription;Transcription regulation;Tumor suppressor;Ubl conjugation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Chromosome Note=Associates with chromatin and mitotic chromosomes. " Q15327,PROTEIN NAMES: Ankyrin repeat domain-containing protein 1 (Cardiac ankyrin repeat protein) (Cytokine-inducible gene C-193 protein) (Cytokine-inducible nuclear protein) ORGANISM: Homo sapiens (Human) FUNCTION: May play an important role in endothelial cell activation. May act as a nuclear transcription factor that negatively regulates the expression of cardiac genes. Induction seems to be correlated with apoptotic cell death in hepatoma cells. KEYWORDS: ANK repeat;Coiled coil;Disease variant;Nucleus;Reference proteome;Repeat SUBCELLULAR LOCATION: Nucleus Q15361,"PROTEIN NAMES: Transcription termination factor 1 (TTF-1) (RNA polymerase I termination factor) (Transcription termination factor I) (TTF-I) ORGANISM: Homo sapiens (Human) FUNCTION: Multifunctional nucleolar protein that terminates ribosomal gene transcription, mediates replication fork arrest and regulates RNA polymerase I transcription on chromatin. Plays a dual role in rDNA regulation, being involved in both activation and silencing of rDNA transcription. Interaction with BAZ2A/TIP5 recovers DNA-binding activity. KEYWORDS: DNA replication inhibitor;DNA-binding;Isopeptide bond;Nucleus;Phosphoprotein;Reference proteome;Repeat;Transcription;Transcription regulation;Transcription termination;Ubl conjugation SUBCELLULAR LOCATION: Nucleus Nucleus, nucleolus Nucleus, nucleoplasm Note=May be localized to the nucleolus in an NPM1/B23-dependent manner. May be displaced from the nucleolus into the nucleoplasm in an CDKN2A/ARF-dependent manner. May shuttle back and forth from nucleoplasm to nucleolus. " Q15392,"PROTEIN NAMES: Delta(24)-sterol reductase (24-dehydrocholesterol reductase) (3-beta-hydroxysterol Delta-24-reductase) (Diminuto/dwarf1 homolog) (Seladin-1) PROTEIN FAMILY: FAD-binding oxidoreductase/transferase type 4 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the FAD-binding oxidoreductase/transferase type 4 family. FUNCTION: Catalyzes the reduction of the delta-24 double bond of sterol intermediates during cholesterol biosynthesis. In addition to its cholesterol-synthesizing activity, can protect cells from oxidative stress by reducing caspase 3 activity during apoptosis induced by oxidative stress. Also protects against amyloid-beta peptide-induced apoptosis. PATHWAY: Steroid biosynthesis; cholesterol biosynthesis. KEYWORDS: Alternative splicing;Cholesterol biosynthesis;Cholesterol metabolism;Disease variant;Endoplasmic reticulum;FAD;Flavoprotein;Golgi apparatus;Lipid biosynthesis;Lipid metabolism;Membrane;NADP;Oxidoreductase;Reference proteome;Signal;Steroid biosynthesis;Steroid metabolism;Sterol biosynthesis;Sterol metabolism;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Single-pass membrane protein Golgi apparatus membrane ; Single-pass membrane protein " Q15397,"PROTEIN NAMES: Pumilio homolog 3 (HBV X-transactivated gene 5 protein) (HBV XAg-transactivated protein 5) (Minor histocompatibility antigen HA-8) (HLA-HA8) ORGANISM: Homo sapiens (Human) FUNCTION: Inhibits the poly(ADP-ribosyl)ation activity of PARP1 and the degradation of PARP1 by CASP3 following genotoxic stress. Binds to double-stranded RNA or DNA without sequence specificity. Involved in development of the eye and of primordial germ cells (By similarity). KEYWORDS: 3D-structure;Acetylation;Chromosome;Direct protein sequencing;DNA-binding;Nucleus;Reference proteome;Repeat;RNA-binding SUBCELLULAR LOCATION: Nucleus, nucleolus Nucleus, nucleoplasm Chromosome Note=Localizes predominantly in the nucleolus with minor punctate signals in the nucleoplasm. " Q15465,"PROTEIN NAMES: Sonic hedgehog protein (SHH) (HHG-1) (Shh unprocessed N-terminal signaling and C-terminal autoprocessing domains) (ShhNC) [Cleaved into: Sonic hedgehog protein N-product (ShhN) (Shh N-terminal processed signaling domains) (ShhNp)] PROTEIN FAMILY: Hedgehog family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the hedgehog family. FUNCTION: [Sonic hedgehog protein]: The C-terminal part of the sonic hedgehog protein precursor displays an autoproteolysis and a cholesterol transferase activity (By similarity). Both activities result in the cleavage of the full-length protein into two parts (ShhN and ShhC) followed by the covalent attachment of a cholesterol moiety to the C-terminal of the newly generated ShhN (By similarity). Both activities occur in the reticulum endoplasmic (By similarity). Once cleaved, ShhC is degraded in the endoplasmic reticulum (By similarity).; FUNCTION: [Sonic hedgehog protein N-product]: The dually lipidated sonic hedgehog protein N-product (ShhNp) is a morphogen which is essential for a variety of patterning events during development. Induces ventral cell fate in the neural tube and somites. Involved in the patterning of the anterior-posterior axis of the developing limb bud (By similarity). Essential for axon guidance (By similarity). Binds to the patched (PTCH1) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. In the absence of SHH, PTCH1 represses the constitutive signaling activity of SMO. KEYWORDS: 3D-structure;Autocatalytic cleavage;Calcium;Cell membrane;Developmental protein;Direct protein sequencing;Disease variant;Endoplasmic reticulum;Glycoprotein;Golgi apparatus;Holoprosencephaly;Hydrolase;Lipoprotein;Membrane;Metal-binding;Microphthalmia;Palmitate;Protease;Reference proteome;Secreted;Signal;Transferase;Zinc SUBCELLULAR LOCATION: [Sonic hedgehog protein]: Endoplasmic reticulum membrane Golgi apparatus membrane Secreted Note=Co-localizes with HHAT in the ER and Golgi membrane.; SUBCELLULAR LOCATION: [Sonic hedgehog protein N-product]: Cell membrane ; Lipid-anchor Note=The dual-lipidated sonic hedgehog protein N-product (ShhNp) is firmly tethered to the cell membrane where it forms multimers. Further solubilization and release from the cell surface seem to be achieved through different mechanisms, including the interaction with DISP1 and SCUBE2, movement by lipoprotein particles, transport by cellular extensions called cytonemes or by the proteolytic removal of both terminal lipidated peptides. " Q15572,"PROTEIN NAMES: TATA box-binding protein-associated factor RNA polymerase I subunit C (RNA polymerase I-specific TBP-associated factor 110 kDa) (TAFI110) (TATA box-binding protein-associated factor 1C) (TBP-associated factor 1C) (Transcription initiation factor SL1/TIF-IB subunit C) ORGANISM: Homo sapiens (Human) FUNCTION: Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (pre-initiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1/TIF-IB with the rDNA promoter. SL1/TIF-IB is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA. Formation of SL1/TIF-IB excludes the association of TBP with TFIID subunits. Recruits RNA polymerase I to the rRNA gene promoter via interaction with RRN3. KEYWORDS: Alternative splicing;Direct protein sequencing;DNA-binding;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus, nucleolus " Q155U0,"PROTEIN NAMES: Vacuolar protein sorting-associated protein 51 homolog (Protein fat-free) PROTEIN FAMILY: VPS51 family ORGANISM: Danio rerio (Zebrafish) (Brachydanio rerio) SIMILARITY: Belongs to the VPS51 family. FUNCTION: Involved in retrograde transport from early and late endosomes to the late Golgi. The GARP complex is required for the maintenance of protein retrieval from endosomes to the TGN, acid hydrolase sorting, lysosome function, endosomal cholesterol traffic and autophagy. Acts as a component of the EARP complex that is involved in endocytic recycling (By similarity). KEYWORDS: Coiled coil;Endosome;Golgi apparatus;Lipid transport;Protein transport;Reference proteome;Transport SUBCELLULAR LOCATION: Golgi apparatus, trans-Golgi network Recycling endosome Note=Also localizes in perinuclear region. Localizes to the trans-Golgi network as part of the GARP complex, while it localizes to recycling endosomes as part of the EARP complex (By similarity). " Q15646,"PROTEIN NAMES: 2'-5'-oligoadenylate synthase-like protein (2'-5'-OAS-related protein) (2'-5'-OAS-RP) (59 kDa 2'-5'-oligoadenylate synthase-like protein) (Thyroid receptor-interacting protein 14) (TR-interacting protein 14) (TRIP-14) (p59 OASL) (p59OASL) PROTEIN FAMILY: 2-5A synthase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the 2-5A synthase family. FUNCTION: Does not have 2'-5'-OAS activity, but can bind double-stranded RNA. Displays antiviral activity against encephalomyocarditis virus (EMCV) and hepatitis C virus (HCV) via an alternative antiviral pathway independent of RNase L. KEYWORDS: 3D-structure;Acetylation;Alternative splicing;Antiviral defense;Cytoplasm;Immunity;Innate immunity;Nucleus;Reference proteome;Repeat;RNA-binding SUBCELLULAR LOCATION: [Isoform p56]: Nucleus, nucleolus. Cytoplasm.; SUBCELLULAR LOCATION: [Isoform p30]: Cytoplasm. MISCELLANEOUS: [Isoform 3]: Has antiviral activity against RNA viruses." Q15696,"PROTEIN NAMES: U2 small nuclear ribonucleoprotein auxiliary factor 35 kDa subunit-related protein 2 (CCCH type zinc finger, RNA-binding motif and serine/arginine rich protein 2) (Renal carcinoma antigen NY-REN-20) (U2(RNU2) small nuclear RNA auxiliary factor 1-like 2) (U2AF35-related protein) (URP) ORGANISM: Homo sapiens (Human) FUNCTION: Pre-mRNA-binding protein required for splicing of both U2- and U12-type introns. Selectively interacts with the 3'-splice site of U2- and U12-type pre-mRNAs and promotes different steps in U2 and U12 intron splicing. Recruited to U12 pre-mRNAs in an ATP-dependent manner and is required for assembly of the prespliceosome, a precursor to other spliceosomal complexes. For U2-type introns, it is selectively and specifically required for the second step of splicing. KEYWORDS: Isopeptide bond;Metal-binding;mRNA processing;mRNA splicing;Nucleus;Phosphoprotein;Reference proteome;Repeat;Ribonucleoprotein;RNA-binding;Spliceosome;Ubl conjugation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus " Q15697,PROTEIN NAMES: Zinc finger protein 174 (AW-1) (Zinc finger and SCAN domain-containing protein 8) PROTEIN FAMILY: Krueppel C2H2-type zinc-finger protein family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein family. FUNCTION: Transcriptional repressor. KEYWORDS: 3D-structure;Alternative splicing;DNA-binding;Isopeptide bond;Metal-binding;Nucleus;Reference proteome;Repeat;Repressor;Transcription;Transcription regulation;Ubl conjugation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus. Q15722,"PROTEIN NAMES: Leukotriene B4 receptor 1 (LTB4-R 1) (LTB4-R1) (Chemoattractant receptor-like 1) (G-protein coupled receptor 16) (P2Y purinoceptor 7) (P2Y7) PROTEIN FAMILY: G-protein coupled receptor 1 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the G-protein coupled receptor 1 family. FUNCTION: Receptor for extracellular ATP > UTP and ADP. The activity of this receptor is mediated by G proteins which activate a phosphatidylinositol-calcium second messenger system. May be the cardiac P2Y receptor involved in the regulation of cardiac muscle contraction through modulation of L-type calcium currents. Is a receptor for leukotriene B4, a potent chemoattractant involved in inflammation and immune response. KEYWORDS: 3D-structure;Cell membrane;G-protein coupled receptor;Glycoprotein;Membrane;Phosphoprotein;Receptor;Reference proteome;Transducer;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. " Q15742,PROTEIN NAMES: NGFI-A-binding protein 2 (EGR-1-binding protein 2) (Melanoma-associated delayed early response protein) (Protein MADER) PROTEIN FAMILY: NAB family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the NAB family. FUNCTION: Acts as a transcriptional repressor for zinc finger transcription factors EGR1 and EGR2. Isoform 2 lacks repression ability (By similarity). KEYWORDS: Alternative splicing;Isopeptide bond;Nucleus;Phosphoprotein;Reference proteome;Repressor;Transcription;Transcription regulation;Ubl conjugation SUBCELLULAR LOCATION: Nucleus Note=Isoform 2 is not localized to the nucleus. Q15743,PROTEIN NAMES: Ovarian cancer G-protein coupled receptor 1 (OGR-1) (G-protein coupled receptor 68) (GPR12A) (Sphingosylphosphorylcholine receptor) PROTEIN FAMILY: G-protein coupled receptor 1 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the G-protein coupled receptor 1 family. FUNCTION: Proton-sensing receptor involved in pH homeostasis. May represents an osteoblastic pH sensor regulating cell-mediated responses to acidosis in bone. Mediates its action by association with G proteins that stimulates inositol phosphate (IP) production or Ca(2+) mobilization. The receptor is almost silent at pH 7.8 but fully activated at pH 6.8. Also functions as a metastasis suppressor gene in prostate cancer (By similarity). KEYWORDS: Amelogenesis imperfecta;Cell membrane;Disease variant;Disulfide bond;G-protein coupled receptor;Glycoprotein;Membrane;Receptor;Reference proteome;Transducer;Transmembrane;Transmembrane helix;Tumor suppressor SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Q15884,"PROTEIN NAMES: Endosomal transmembrane epsin interactor 1 (Endosomal transmembrane binding with epsin) PROTEIN FAMILY: ENTREP family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the ENTREP family. FUNCTION: Functions as an activator of the E3 ubiquitin protein ligase ITCH in the ubiquitination of the CXCL12-activated CXCR4 receptor. Thereby, triggers CXCR4 endocytosis and desensitization, negatively regulating the CXCL12/CXCR4 signaling pathway. KEYWORDS: Alternative splicing;Cell membrane;Endosome;Isopeptide bond;Membrane;Phosphoprotein;Reference proteome;Signal;Transmembrane;Transmembrane helix;Ubl conjugation SUBCELLULAR LOCATION: Early endosome membrane ; Single-pass type I membrane protein Late endosome membrane ; Single-pass type I membrane protein Recycling endosome membrane ; Single-pass type I membrane protein Cell membrane ; Single-pass type I membrane protein Note=Enriched in endosomes compared to the cell membrane. " Q15904,"PROTEIN NAMES: V-type proton ATPase subunit S1 (V-ATPase subunit S1) (Protein XAP-3) (V-ATPase Ac45 subunit) (V-ATPase S1 accessory protein) (Vacuolar proton pump subunit S1) PROTEIN FAMILY: Vacuolar ATPase subunit S1 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the vacuolar ATPase subunit S1 family. FUNCTION: Accessory subunit of the proton-transporting vacuolar (V)-ATPase protein pump, which is required for luminal acidification of secretory vesicles. Guides the V-type ATPase into specialized subcellular compartments, such as neuroendocrine regulated secretory vesicles or the ruffled border of the osteoclast, thereby regulating its activity. Involved in membrane trafficking and Ca(2+)-dependent membrane fusion. May play a role in the assembly of the V-type ATPase complex (Probable). In aerobic conditions, involved in intracellular iron homeostasis, thus triggering the activity of Fe(2+) prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation. In islets of Langerhans cells, may regulate the acidification of dense-core secretory granules (By similarity). KEYWORDS: 3D-structure;Congenital disorder of glycosylation;Cytoplasmic vesicle;Disease variant;Disulfide bond;Endoplasmic reticulum;Glycoprotein;Hydrogen ion transport;Ion transport;Membrane;Phosphoprotein;Reference proteome;Signal;Synapse;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Single-pass type I membrane protein Endoplasmic reticulum-Golgi intermediate compartment membrane Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane ; Single-pass type I membrane protein Cytoplasmic vesicle, clathrin-coated vesicle membrane ; Single-pass type I membrane protein Note=Not detected in trans-Golgi network. " Q15942,"PROTEIN NAMES: Zyxin (Zyxin-2) PROTEIN FAMILY: Zyxin/ajuba family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the zyxin/ajuba family. FUNCTION: Adhesion plaque protein. Binds alpha-actinin and the CRP protein. Important for targeting TES and ENA/VASP family members to focal adhesions and for the formation of actin-rich structures. May be a component of a signal transduction pathway that mediates adhesion-stimulated changes in gene expression (By similarity). KEYWORDS: Acetylation;Alternative splicing;Cell adhesion;Cell junction;Cytoplasm;Cytoskeleton;Direct protein sequencing;Host-virus interaction;LIM domain;Metal-binding;Methylation;Nucleus;Phosphoprotein;Reference proteome;Repeat;Zinc SUBCELLULAR LOCATION: Cytoplasm. Cytoplasm, cytoskeleton. Nucleus. Cell junction, focal adhesion. Note=Associates with the actin cytoskeleton near the adhesion plaques. Enters the nucleus in the presence of HESX1. " Q16342,PROTEIN NAMES: Programmed cell death protein 2 (Zinc finger MYND domain-containing protein 7) (Zinc finger protein Rp-8) ORGANISM: Homo sapiens (Human) FUNCTION: May be a DNA-binding protein with a regulatory function. May play an important role in cell death and/or in regulation of cell proliferation. KEYWORDS: Alternative splicing;Apoptosis;DNA-binding;Metal-binding;Nucleus;Reference proteome;Ubl conjugation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Q16478,"PROTEIN NAMES: Glutamate receptor ionotropic, kainate 5 (GluK5) (Excitatory amino acid receptor 2) (EAA2) (Glutamate receptor KA-2) (KA2) PROTEIN FAMILY: Glutamate-gated ion channel family, GRIK5 subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIK5 subfamily. FUNCTION: Receptor for glutamate. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. This receptor binds kainate > quisqualate > domoate > L-glutamate >> AMPA >> NMDA = 1S,3R-ACPD. KEYWORDS: Alternative splicing;Cell membrane;Disulfide bond;Glycoprotein;Ion channel;Ion transport;Ligand-gated ion channel;Membrane;Postsynaptic cell membrane;Receptor;Reference proteome;Signal;Synapse;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Postsynaptic cell membrane; Multi-pass membrane protein. " Q16587,PROTEIN NAMES: Zinc finger protein 74 (Zinc finger protein 520) (hZNF7) PROTEIN FAMILY: Krueppel C2H2-type zinc-finger protein family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein family. FUNCTION: May play a role in RNA metabolism. KEYWORDS: Alternative splicing;DNA-binding;Isopeptide bond;Metal-binding;Nucleus;Reference proteome;Repeat;RNA-binding;Transcription;Transcription regulation;Ubl conjugation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus. Q16637,"PROTEIN NAMES: Survival motor neuron protein (Component of gems 1) (Gemin-1) PROTEIN FAMILY: SMN family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the SMN family. FUNCTION: The SMN complex catalyzes the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome, and thereby plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP (Sm core). In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. To assemble core snRNPs, the SMN complex accepts the trapped 5Sm proteins from CLNS1A forming an intermediate. Within the SMN complex, SMN1 acts as a structural backbone and together with GEMIN2 it gathers the Sm complex subunits. Binding of snRNA inside 5Sm ultimately triggers eviction of the SMN complex, thereby allowing binding of SNRPD3 and SNRPB to complete assembly of the core snRNP. Ensures the correct splicing of U12 intron-containing genes that may be important for normal motor and proprioceptive neurons development. Also required for resolving RNA-DNA hybrids created by RNA polymerase II, that form R-loop in transcription terminal regions, an important step in proper transcription termination. May also play a role in the metabolism of small nucleolar ribonucleoprotein (snoRNPs). KEYWORDS: 3D-structure;Acetylation;Alternative splicing;Cell projection;Cytoplasm;Disease variant;Isopeptide bond;mRNA processing;mRNA splicing;Neurodegeneration;Neurogenesis;Nucleus;Phosphoprotein;Reference proteome;RNA-binding;Ubl conjugation SUBCELLULAR LOCATION: Nucleus, gem Nucleus, Cajal body Cytoplasm Cytoplasmic granule Perikaryon Cell projection, neuron projection Cell projection, axon Cytoplasm, myofibril, sarcomere, Z line Note=Colocalizes with actin and at the Z-line of skeletal muscle (By similarity). Under stress conditions colocalizes with RPP20/POP7 in punctuated cytoplasmic granules. Colocalized and redistributed with ZPR1 from the cytoplasm to nuclear gems (Gemini of coiled bodies) and Cajal bodies. Colocalizes with FMR1 in cytoplasmic granules in the soma and neurite cell processes. MISCELLANEOUS: The SMN gene is present in two highly homologous and functional copies (TelSMN/SMN1 and CenSMN/SMN2). The telomeric copy of SMN gene (TelSMN/SMN1) seems to be the SMA-determining gene while the centromeric copy seems unaffected.; MISCELLANEOUS: [Isoform SMN]: Primarily derived from SMN1 gene.; MISCELLANEOUS: [Isoform SMN-delta7]: Thought to be a non-functional protein that lacks the capacity to oligomerize and thus cannot interact with Sm proteins. Primarily derived from SMN2 gene." Q16653,"PROTEIN NAMES: Myelin-oligodendrocyte glycoprotein PROTEIN FAMILY: Immunoglobulin superfamily, BTN/MOG family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the immunoglobulin superfamily. BTN/MOG family. FUNCTION: Mediates homophilic cell-cell adhesion (By similarity). Minor component of the myelin sheath. May be involved in completion and/or maintenance of the myelin sheath and in cell-cell communication.; FUNCTION: (Microbial infection) Acts as a receptor for rubella virus. KEYWORDS: Alternative splicing;Cell adhesion;Cell membrane;Disease variant;Disulfide bond;Glycoprotein;Host cell receptor for virus entry;Host-virus interaction;Immunoglobulin domain;Membrane;Receptor;Reference proteome;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: [Isoform 1]: Cell membrane ; Multi-pass membrane protein.; SUBCELLULAR LOCATION: [Isoform 5]: Cell membrane ; Multi-pass membrane protein.; SUBCELLULAR LOCATION: [Isoform 2]: Cell membrane ; Single-pass type I membrane protein.; SUBCELLULAR LOCATION: [Isoform 3]: Cell membrane ; Single-pass type I membrane protein.; SUBCELLULAR LOCATION: [Isoform 4]: Cell membrane ; Single-pass type I membrane protein.; SUBCELLULAR LOCATION: [Isoform 6]: Cell membrane ; Single-pass type I membrane protein.; SUBCELLULAR LOCATION: [Isoform 7]: Cell membrane ; Single-pass type I membrane protein.; SUBCELLULAR LOCATION: [Isoform 8]: Cell membrane ; Single-pass type I membrane protein.; SUBCELLULAR LOCATION: [Isoform 9]: Cell membrane ; Single-pass type I membrane protein MISCELLANEOUS: [Isoform 9]: Not functionally active. May be expressed at low level in the adult.; MISCELLANEOUS: [Isoform 10]: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay." Q16659,"PROTEIN NAMES: Mitogen-activated protein kinase 6 (MAP kinase 6) (MAPK 6) (Extracellular signal-regulated kinase 3) (ERK-3) (MAP kinase isoform p97) (p97-MAPK) PROTEIN FAMILY: Protein kinase superfamily, CMGC Ser/Thr protein kinase family, MAP kinase subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. FUNCTION: Atypical MAPK protein. Phosphorylates microtubule-associated protein 2 (MAP2) and MAPKAPK5. The precise role of the complex formed with MAPKAPK5 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPKAPK5, ERK3/MAPK6 is phosphorylated at Ser-189 and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK3/MAPK6. May promote entry in the cell cycle (By similarity). KEYWORDS: 3D-structure;ATP-binding;Cell cycle;Cytoplasm;Kinase;Nucleotide-binding;Nucleus;Phosphoprotein;Reference proteome;Serine/threonine-protein kinase;Transferase;Ubl conjugation SUBCELLULAR LOCATION: Cytoplasm Nucleus Note=Translocates to the cytoplasm following interaction with MAPKAPK5. " Q16698,"PROTEIN NAMES: 2,4-dienoyl-CoA reductase [(3E)-enoyl-CoA-producing], mitochondrial (2,4-dienoyl-CoA reductase [NADPH]) (4-enoyl-CoA reductase [NADPH]) (Short chain dehydrogenase/reductase family 18C member 1) PROTEIN FAMILY: Short-chain dehydrogenases/reductases (SDR) family, 2,4-dienoyl-CoA reductase subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR) family. 2,4-dienoyl-CoA reductase subfamily. FUNCTION: Auxiliary enzyme of beta-oxidation. It participates in the metabolism of unsaturated fatty enoyl-CoA esters having double bonds in both even- and odd-numbered positions in mitochondria. Catalyzes the NADP-dependent reduction of 2,4-dienoyl-CoA to yield trans-3-enoyl-CoA. KEYWORDS: 3D-structure;Acetylation;Alternative splicing;Fatty acid metabolism;Lipid metabolism;Mitochondrion;NADP;Oxidoreductase;Phosphoprotein;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Mitochondrion " Q16891,"PROTEIN NAMES: MICOS complex subunit MIC60 (Cell proliferation-inducing gene 4/52 protein) (Mitochondrial inner membrane protein) (Mitofilin) (p87/89) PROTEIN FAMILY: MICOS complex subunit Mic60 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the MICOS complex subunit Mic60 family. FUNCTION: Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane. Plays an important role in the maintenance of the MICOS complex stability and the mitochondrial cristae morphology. KEYWORDS: Acetylation;Alternative splicing;Direct protein sequencing;Host-virus interaction;Membrane;Mitochondrion;Mitochondrion inner membrane;Phosphoprotein;Reference proteome;Transit peptide;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Mitochondrion inner membrane ; Single-pass membrane protein Mitochondrion " Q16975,"PROTEIN NAMES: Calcium-independent protein kinase C (APL II) PROTEIN FAMILY: Protein kinase superfamily, AGC Ser/Thr protein kinase family, PKC subfamily ORGANISM: Aplysia californica (California sea hare) SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. FUNCTION: This is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme.; FUNCTION: PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters (By similarity). KEYWORDS: ATP-binding;Kinase;Membrane;Metal-binding;Nucleotide-binding;Phosphoprotein;Repeat;Serine/threonine-protein kinase;Transferase;Zinc;Zinc-finger SUBCELLULAR LOCATION: Membrane; Peripheral membrane protein. " Q17328,"PROTEIN NAMES: Glutamate-gated chloride channel subunit beta (Glu-Cl subunit beta) (Avermectin-sensitive glutamate-gated chloride channel subunit) PROTEIN FAMILY: Ligand-gated ion channel family, Glutamate-gated chloride channel subfamily ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the ligand-gated ion channel (TC 1.A.9) family. Glutamate-gated chloride channel (TC 1.A.9.4) subfamily. FUNCTION: Glutamate-gated chloride channel subunit; channel properties depend on the subunit composition. Glutamate binding triggers a rapidly reversible current in heteromeric channels formed by glc-1 and glc-2, while the anti-helmintic drug ivermectin and other avermectins trigger a permanently open channel configuration. Channels containing only glc-2 are activated by glutamate, but not by ivermectin (in vitro). The heteromeric channel formed by glc-1 and glc-2 is also activated by ibotenate, and it is blocked by picrotoxin and flufenamic acid. KEYWORDS: Cell membrane;Chloride;Chloride channel;Disulfide bond;Glycoprotein;Ion channel;Ion transport;Ligand-gated ion channel;Membrane;Postsynaptic cell membrane;Receptor;Reference proteome;Signal;Synapse;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Postsynaptic cell membrane ; Multi-pass membrane protein Cell membrane ; Multi-pass membrane protein " Q17381,"PROTEIN NAMES: Defective pharyngeal development protein 4 (Ce-fkh-1) (Fork head-HNF-3 homolog) ORGANISM: Caenorhabditis elegans FUNCTION: Acts as a transcription factor required for formation of the pharyngeal primordium. Binds to the consensus sequence 5'-T[AG]TT[TG][AG][TC]-3' with variations of the sequence affecting onset of target gene expression. Becomes associated with pharyngeal target promoters by the 8E cell stage, playing a role in inducing large-scale chromatin decompaction, prior to the activation of transcription. Binding with target sequences in pharyngeal cells may be repressed by the nuclear lamina protein emr-1. Activates a wide array of pharyngeal genes including ceh-22, pax-1 and myo-2 and represses ectodermal genes lin-26 and elt-3, ensuring pharyngeal cell fate. Required for recruitment of htz-1 to a subset of pharyngeal promoters to ensure gene activation and also acts synergistically with tbx-2 in pharyngeal development. Acts in the regulation of diet-restriction-mediated longevity by increasing expression of sod-1, sod-2, sod-4 and sod-5 but not sod-3. Positively regulates the expression of the transcription factor hlh-6 in the pharyngeal gland. May regulate gene expression in the intestine. KEYWORDS: Activator;Alternative splicing;Coiled coil;Developmental protein;DNA-binding;Nucleus;Reference proteome;Repressor;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus Note=Present in nuclei of all cells of larval and adult pharynx but variable in neuronal nuclei. " Q17389,PROTEIN NAMES: Cullin-1 (CUL-1) (Abnormal cell lineage protein 19) PROTEIN FAMILY: Cullin family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the cullin family. FUNCTION: Probable core component of multiple cullin-RING-based SCF (SKP1-CUL1-F-box) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Required for developmentally programmed transitions from the G1 phase of the cell cycle to the G0 phase or the apoptotic pathway. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Cell cycle;Cell division;Cytoplasm;Isopeptide bond;Reference proteome;Ubl conjugation;Ubl conjugation pathway SUBCELLULAR LOCATION: Cytoplasm Q17449,"PROTEIN NAMES: Fatty acid amide hydrolase 1 (Anandamide amidohydrolase 1) PROTEIN FAMILY: Amidase family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the amidase family. FUNCTION: Catalyzes the hydrolysis of endogenous amidated lipids like anandamide (AEA or N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-ethanolamine) and eicosapentaneoyl ethanolamide (EPEA or (5Z,8Z,11Z,14Z,17Z-eicosapentaenoyl) ethanolamine), as well as other fatty amides, to their corresponding fatty acids, thereby regulating the signaling functions of these molecules. EPEA promotes dauer formation and may constitute a signal of high nutrient availability. Breakdown of EPEA may promote lifespan extension when nutrient availability is high. Facilitates axon regeneration after injury by degradating inhibitory compounds such as AEA. FAAH cooperates with PM20D1 in the hydrolysis of amino acid-conjugated fatty acids such as N-fatty acyl glycine and N-fatty acyl-L-serine, thereby acting as a physiological regulator of specific subsets of intracellular, but not of extracellular, N-fatty acyl amino acids (By similarity). KEYWORDS: Coiled coil;Hydrolase;Lipid degradation;Lipid metabolism;Phosphoprotein;Reference proteome;Signal " Q17635,"PROTEIN NAMES: Kinetochore protein ndc-80 (CeNDC-80) (Hec1 homolog) PROTEIN FAMILY: NDC80/HEC1 family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the NDC80/HEC1 family. FUNCTION: Acts as a component of the essential kinetochore-associated ndc-80 complex, which is required for chromosome segregation in mitosis and meiosis and spindle checkpoint activity. Plays a role in kinetochore assembly and recruits the checkpoint protein mdf-2 and the spindly-like protein spdl-1 to unattached kinetochores. Mediates the formation of end-on kinetochore-microtubule attachments through recruitment of spdl-1. The ndc-80 complex synergistically enhances the affinity of the ska-1 complex for microtubules and may allow the ndc-80 complex to track depolymerizing microtubules. KEYWORDS: Cell cycle;Cell division;Centromere;Chromosome;Coiled coil;Cytoplasm;Cytoskeleton;Kinetochore;Meiosis;Mitosis;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus Chromosome, centromere, kinetochore Cytoplasm, cytoskeleton Note=Localizes to kinetochores. The NDC80 complex localizes to microtubules. " Q17CS8,"PROTEIN NAMES: Kynurenine aminotransferase (AeKAT) PROTEIN FAMILY: Class-I pyridoxal-phosphate-dependent aminotransferase family ORGANISM: Aedes aegypti (Yellowfever mosquito) (Culex aegypti) SIMILARITY: Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. FUNCTION: Catalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid (KA). Also catalyzes the irreversible transamination of several amino acids including cysteine, tyrosine, glutamine, methionine, histidine and phenylalanine. Can use various keto-acids as the amino group acceptor. PATHWAY: Amino-acid degradation; L-kynurenine degradation; kynurenate from L-kynurenine: step 1/2. KEYWORDS: 3D-structure;Aminotransferase;Glycoprotein;Mitochondrion;Pyridoxal phosphate;Reference proteome;Transferase;Transit peptide SUBCELLULAR LOCATION: Mitochondrion " Q17RS7,"PROTEIN NAMES: Flap endonuclease GEN homolog 1 PROTEIN FAMILY: XPG/RAD2 endonuclease family, GEN subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the XPG/RAD2 endonuclease family. GEN subfamily. FUNCTION: Endonuclease which resolves Holliday junctions (HJs) by the introduction of symmetrically related cuts across the junction point, to produce nicked duplex products in which the nicks can be readily ligated. Four-way DNA intermediates, also known as Holliday junctions, are formed during homologous recombination and DNA repair, and their resolution is necessary for proper chromosome segregation. Cleaves HJs by a nick and counter-nick mechanism involving dual coordinated incisions that lead to the formation of ligatable nicked duplex products. Cleavage of the first strand is rate limiting, while second strand cleavage is rapid. Largely monomeric, dimerizes on the HJ and the first nick occurs upon dimerization at the junction. Efficiently cleaves both single and double HJs contained within large recombination intermediates. Exhibits a weak sequence preference for incision between two G residues that reside in a T-rich region of DNA. Has also endonuclease activity on 5'-flap and replication fork (RF) DNA substrates. KEYWORDS: 3D-structure;DNA damage;DNA repair;Endonuclease;Hydrolase;Magnesium;Metal-binding;Nuclease;Nucleus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Nucleus " Q17RY0,"PROTEIN NAMES: Cytoplasmic polyadenylation element-binding protein 4 (CPE-BP4) (CPE-binding protein 4) (hCPEB-4) PROTEIN FAMILY: RRM CPEB family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the RRM CPEB family. FUNCTION: Sequence-specific RNA-binding protein that binds to the cytoplasmic polyadenylation element (CPE), an uridine-rich sequence element (consensus sequence 5'-UUUUUAU-3') within the mRNA 3'-UTR. RNA binding results in a clear conformational change analogous to the Venus fly trap mechanism. Regulates activation of unfolded protein response (UPR) in the process of adaptation to ER stress in liver, by maintaining translation of CPE-regulated mRNAs in conditions in which global protein synthesis is inhibited (By similarity). Required for cell cycle progression, specifically for cytokinesis and chromosomal segregation. Plays a role as an oncogene promoting tumor growth and progression by positively regulating translation of t-plasminogen activator/PLAT. Stimulates proliferation of melanocytes. In contrast to CPEB1 and CPEB3, does not play role in synaptic plasticity, learning and memory (By similarity). KEYWORDS: 3D-structure;Alternative splicing;Cell projection;Cytoplasm;Endoplasmic reticulum;Metal-binding;Phosphoprotein;Reference proteome;Repeat;RNA-binding;Synapse;Zinc SUBCELLULAR LOCATION: Cytoplasm Cell projection, dendrite Cell projection, dendritic spine Postsynaptic density Cell projection, axon Cell projection, growth cone Endoplasmic reticulum Cytoplasm, perinuclear region " Q18007,"PROTEIN NAMES: Probable muscarinic acetylcholine receptor gar-1 (G-protein-linked acetylcholine receptor 1) PROTEIN FAMILY: G-protein coupled receptor 1 family, Muscarinic acetylcholine receptor subfamily ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. FUNCTION: The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover. KEYWORDS: Alternative splicing;Cell membrane;Disulfide bond;G-protein coupled receptor;Glycoprotein;Membrane;Receptor;Reference proteome;Transducer;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. " Q18020,PROTEIN NAMES: Aspartic protease 10 (Heme transporter hrg-7) (Heme-responsive gene 7 protein) PROTEIN FAMILY: Peptidase A1 family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the peptidase A1 family. FUNCTION: Aspartic protease which plays a role in heme homeostasis and mediates inter-organ signaling between the intestine and extra-intestinal tissues when cellular heme levels are low. KEYWORDS: Alternative splicing;Aspartyl protease;Disulfide bond;Glycoprotein;Hydrolase;Protease;Reference proteome;Secreted;Signal;Zymogen SUBCELLULAR LOCATION: Secreted Q182W3,PROTEIN NAMES: Stage IV sporulation protein A (Coat morphogenetic protein SpoIVA) ORGANISM: Clostridioides difficile (strain 630) (Peptoclostridium difficile) FUNCTION: ATPase. Has a role at an early stage in the morphogenesis of the spore coat and is required for proper coat localization to the forespore. KEYWORDS: ATP-binding;Coiled coil;Cytoplasm;Hydrolase;Nucleotide-binding;Reference proteome;Sporulation SUBCELLULAR LOCATION: Cytoplasm Note=Localized on spore coat surrounding the forespore. Q183R7,"PROTEIN NAMES: Pro-Pro endopeptidase (PPEP-1) (Zinc metalloprotease Zmp1) PROTEIN FAMILY: Peptidase M34 family, Pro-Pro endopeptidase subfamily ORGANISM: Clostridioides difficile (strain 630) (Peptoclostridium difficile) SIMILARITY: Belongs to the peptidase M34 family. Pro-Pro endopeptidase subfamily. FUNCTION: Zinc-dependent endoprotease with a unique preference for proline residues surrounding the scissile bond. Exhibits a high preference for an asparagine at the P2 position and hydrophobic residues (Val, Ile, Leu) at the P3 position. Efficiently cleaves the LPXTG cell surface proteins CD630_28310 and CD630_32460 at multiple cleavage sites in vivo. Has a role in the regulation of C.difficile adhesion versus motility by cleaving surface adhesion proteins such as the collagen binding protein CD630_28310, and is important for efficient infection. Is also able to cleave fibronectin and fibrinogen in vitro; cleaves at the N-terminus of the beta-chain of fibrinogen. Destabilizes the fibronectin network produced by human fibroblasts. Therefore, may be important in key steps of clostridial pathogenesis by degrading extracellular matrix components associated with the gut epithelial cells. To a lesser extent, IgA1, IgA2, and human HSP 90-beta, but not HSP 90-alpha, are also substrates for the enzyme. Is not active on different collagen types, casein and gelatin. KEYWORDS: 3D-structure;Hydrolase;Metal-binding;Metalloprotease;Protease;Reference proteome;Secreted;Signal;Virulence;Zinc SUBCELLULAR LOCATION: Secreted " Q18425,"PROTEIN NAMES: Sphingosine kinase 1 ORGANISM: Caenorhabditis elegans FUNCTION: Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on sphinganine (By similarity). Required for neurotransmitter release from neuromuscular junctions. Acts by recruiting the synaptic vesicle priming protein unc-13 to synapses (Probable). PATHWAY: Lipid metabolism; sphingolipid metabolism. KEYWORDS: Alternative splicing;ATP-binding;Cell membrane;Cell projection;Kinase;Lipid metabolism;Membrane;Mitochondrion;Nucleotide-binding;Reference proteome;Sphingolipid metabolism;Synapse;Transferase SUBCELLULAR LOCATION: Presynaptic cell membrane Cell projection, axon Perikaryon. Mitochondrion membrane. Note=Recruitment to the presynaptic membrane may be regulated by endogenous acetylcholine, which activates a muscarinic signaling pathway acting through gar-3, egl-30 and unc-73. Muscarinic-mediated recruitment of sphk-1 to presynaptic terminals requires calcium influx and the calcium-binding protein calm-1. Primarily observed on the outer surface of mitochondria in body wall muscles. " Q18685,"PROTEIN NAMES: Protein unc-112 (Mitogen-inducible mig-2 protein-like) (Uncoordinated protein 112) PROTEIN FAMILY: Kindlin family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the kindlin family. FUNCTION: Component of an integrin containing attachment complex, which is required for muscle development and maintenance. Probable regulator of cell-extracellular matrix adhesion. Required during initial muscle assembly to form dense bodies and M-lines. KEYWORDS: Cell adhesion;Cell membrane;Cytoplasm;Membrane;Reference proteome SUBCELLULAR LOCATION: Cell membrane ; Peripheral membrane protein Cytoplasm, myofibril, sarcomere, M line Note=Colocalizes with pat-3/beta-integrin in body wall muscles. Requires unc-52/perlecan and pat-3 to be localized to the muscle cell membrane. Colocalizes with cpna-1 in M line and dense bodies. " Q18788,"PROTEIN NAMES: Mannosyl-oligosaccharide 1,2-alpha-mannosidase C52E4.5 (Processing alpha-1,2-mannosidase C52E4.5) (Alpha-1,2-mannosidase C52E4.5) PROTEIN FAMILY: Glycosyl hydrolase 47 family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the glycosyl hydrolase 47 family. FUNCTION: Involved in the maturation of Asn-linked oligosaccharides. Progressively trim alpha-1,2-linked mannose residues from Man(9)GlcNAc(2) to produce Man(5)GlcNAc(2) (By similarity). PATHWAY: Protein modification; protein glycosylation. KEYWORDS: Alternative splicing;Calcium;Disulfide bond;Glycoprotein;Glycosidase;Hydrolase;Membrane;Metal-binding;Reference proteome;Signal-anchor;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane; Single-pass type II membrane protein. " Q18806,"PROTEIN NAMES: Neuronal immunoglobulin domain-containing protein rig-3 ORGANISM: Caenorhabditis elegans FUNCTION: Cell surface protein which plays a role in the plasticity of cholinergic synapses at neuromuscular junctions and in the polarity of the mechanosensory neuron ALM, possibly by antagonizing Wnt signaling. KEYWORDS: Cell membrane;Cell projection;Disulfide bond;Glycoprotein;GPI-anchor;Immunoglobulin domain;Lipoprotein;Membrane;Reference proteome;Repeat;Signal;Synapse SUBCELLULAR LOCATION: Cell projection, axon Synapse Cell membrane ; Lipid-anchor, GPI-anchor Note=Membrane association is important for synaptic function. " Q18812,PROTEIN NAMES: Acetylcholine-gated chloride channel subunit acc-2 PROTEIN FAMILY: Ligand-gated ion channel family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the ligand-gated ion channel (TC 1.A.9) family. FUNCTION: Acetylcholine-gated chloride channel subunit. Currents in channels are triggered in response to acetylcholine. Channel properties may be modulated by the formation of homomeric and heteromeric channels. KEYWORDS: Cell membrane;Chloride;Chloride channel;Disulfide bond;Glycoprotein;Ion channel;Ion transport;Ligand-gated ion channel;Membrane;Reference proteome;Signal;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Q18825,"PROTEIN NAMES: Beta-catenin/armadillo-related protein 1 (Protruding vulva protein 1) (Suppressor of polyray 1) PROTEIN FAMILY: Beta-catenin family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the beta-catenin family. FUNCTION: Participates in the Wnt signaling pathway which affects cell fate and may regulate the stem cell divisions of seam cells during larval development. Functions as a transcriptional activator but is dependent on the interaction with pop-1. Involved in maintaining lin-39 Hox expression and regulating glr-1 abundance at the synapses. Required for mab-5 expression during Q neuroblast migration and for oxidative stress-induced daf-16 signaling. Has roles in egg laying, vulva precursor cell fate determination, Q neuroblast migration, posterior ectodermal cell P12 specification, movement, body length, male tail development and dauer induction. Functionally redundant to wrm-1 and hmp-2. In the intestine, plays a role in probiotic-mediated protection against infections by pathogens such as S.enterica. KEYWORDS: Activator;Cell junction;Cytoplasm;Developmental protein;Membrane;Nucleus;Reference proteome;Repeat;Transcription;Transcription regulation;Wnt signaling pathway SUBCELLULAR LOCATION: Cytoplasm Nucleus Membrane Cell junction Note=Mostly cytoplasmic. " Q18879,"PROTEIN NAMES: Caveolin-2 PROTEIN FAMILY: Caveolin family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the caveolin family. FUNCTION: May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can regulate their activity. Thought to have a role in the uptake of lipids and proteins in the intestinal cells; operates in the apical uptake of lipid markers and trafficking of yolk proteins. Affects fecundity and egg laying. KEYWORDS: Alternative splicing;Cell membrane;Golgi apparatus;Membrane;Reference proteome SUBCELLULAR LOCATION: Golgi apparatus membrane ; Peripheral membrane protein Cell membrane ; Peripheral membrane protein Membrane, caveola ; Peripheral membrane protein Apical cell membrane Note=Potential hairpin-like structure in the membrane. Membrane protein of caveolae (By similarity). " Q19203,"PROTEIN NAMES: Zinc finger protein unc-98 (Uncoordinated protein 98) ORGANISM: Caenorhabditis elegans FUNCTION: Probable transcription factor required for muscle structure. Its dual subcellular localization suggests that it may function both as a muscle adhesion complex protein and as a transcription factor, or work together with transcription factors, to influence gene expression. Thought to act as a molecular bridge between unc-97 and myo-3 at the M-line of muscles, possibly in a signaling role. Plays a role in the formation of muscle connections, also called muscle arm extensions, between the body wall and the motor axons in the dorsal and ventral cord. KEYWORDS: Cytoplasm;Developmental protein;Differentiation;DNA-binding;Metal-binding;Muscle protein;Myogenesis;Nucleus;Reference proteome;Repeat;Thick filament;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Cytoplasm Note=Localized in body wall muscle M-lines, dense bodies and muscle nuclei. " Q19267,"PROTEIN NAMES: Protein disulfide isomerase crld-1 (Cysteine-rich with EGF-like domain protein crld-1) PROTEIN FAMILY: CRELD family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the CRELD family. FUNCTION: Protein disulfide isomerase which associates with the unc-29 subunit of levamisole-sensitive nicotinic acetylcholine receptors (L-nAChR) to promote L-nAChR assembly in the endoplasmic reticulum at neuromuscular junctions.; FUNCTION: [Isoform a]: Promotes L-nAChR assembly in the endoplasmic reticulum at neuromuscular junctions. KEYWORDS: Alternative splicing;Calcium;Disulfide bond;EGF-like domain;Endoplasmic reticulum;Glycoprotein;Isomerase;Membrane;Redox-active center;Reference proteome;Repeat;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: [Isoform a]: Endoplasmic reticulum membrane ; Multi-pass membrane protein.; SUBCELLULAR LOCATION: [Isoform b]: Endoplasmic reticulum lumen Note=Ends with a KDEL sequence, which is an endoplasmic reticulum retention signal. " Q19325,"PROTEIN NAMES: Sister chromatid cohesion protein 1 PROTEIN FAMILY: Rad21 family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the rad21 family. FUNCTION: Cleavable component of the cohesin complex involved in chromosome cohesion during cell cycle. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped (By similarity). At metaphase-anaphase transition, this protein is cleaved and dissociates from chromatin, allowing sister chromatids to segregate (By similarity). KEYWORDS: Cell cycle;Cell division;Chromosome;Cytoplasm;Mitosis;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus Chromosome Cytoplasm Note=Shows cell-cycle dependent localization to chromosomes, with an accumulation on chromosomes apparent during mitotic interphase and telephase, but more diffuse nuclear expression during prometaphase, metaphase and anaphase. " Q19427,"PROTEIN NAMES: NAD-capped RNA hydrolase ndx-9 (DeNADding enzyme ndx-9) (NADH pyrophosphatase) (Nudix hydrolase 9) PROTEIN FAMILY: Nudix hydrolase family, NudC subfamily ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the Nudix hydrolase family. NudC subfamily. FUNCTION: mRNA decapping enzyme that specifically removes the nicotinamide adenine dinucleotide (NAD) cap from a subset of mRNAs by hydrolyzing the diphosphate linkage to produce nicotinamide mononucleotide (NMN) and 5' monophosphate mRNA. The NAD-cap is present at the 5'-end of some RNAs; in contrast to the canonical N7 methylguanosine (m7G) cap, the NAD cap promotes mRNA decay (By similarity). Mediates the hydrolysis of some nucleoside diphosphate derivatives. KEYWORDS: Hydrolase;Magnesium;Manganese;Metal-binding;NAD;Reference proteome;Zinc " Q19614,"PROTEIN NAMES: Schlafen-like protein 2 (SLFL-2) (SLFN-like 2) (21U-RNA biogenesis fouled up protein 2) (PUCH complex member slfl-2) (Protein tofu-2) PROTEIN FAMILY: Schlafen family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the Schlafen family. FUNCTION: Component of the trimeric PUCH (precursor of 21U RNA 5'-end cleavage holoenzyme) complex, that acts as an endoribonuclease processing the 5'-end of precursor Piwi-interacting RNAs (piRNAs). The PUCH complex consists of tofu-1, tofu-2 and either slfl-3 or slfl-4, with tofu-2 exhibiting endoribonuclease activity. PUCH-mediated processing strictly requires a 7-methyl-G cap (m7 G-cap) and an uracil at position three (U3). PUCH also exhibits a strict bias for piRNA precursors with an A or G at position 1. Mature piRNA production is enhanced by the interaction of PUCH with the PETISCO complex, which is stabilizing piRNA precursors and allows their processing by PUCH. KEYWORDS: Cytoplasm;Endonuclease;Hydrolase;Mitochondrion;Nuclease;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Mitochondrion Note=The localization to the mitochondrion is mediated by slfl-3. " Q19775,"PROTEIN NAMES: Protein phosphatase ppm-1.A (Protein phosphatase 2C 1) (Protein phosphatase Mg2+/Mn2+ dependent 1) PROTEIN FAMILY: PP2C family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the PP2C family. FUNCTION: Probable phosphatase which regulates axon termination in ALM and PLM neurons, and synaptic branch extension and/or stabilization in PLM neurons. Plays a role in synapse formation in GABAergic DD motor neurons probably by dephosphorylating pmk-3 thereby negatively regulating a MAP kinase pathway that includes dlk-1, mkk-4 and pmk-3. KEYWORDS: Alternative splicing;Hydrolase;Magnesium;Manganese;Metal-binding;Protein phosphatase;Reference proteome;Synapse SUBCELLULAR LOCATION: Synapse Note=Localizes to presynaptic terminals of motor neurons in the dorsal nerve cord. " Q19848,"PROTEIN NAMES: Serine/threonine-protein kinase VRK1 (Vaccinia-related kinase 1) PROTEIN FAMILY: Protein kinase superfamily, CK1 Ser/Thr protein kinase family, VRK subfamily ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. VRK subfamily. FUNCTION: Serine/threonine kinase that phosphorylates baf-1, thus regulating the association of baf-1 with chromatin and nuclear membrane proteins during nuclear envelope formation. May act through the egl-17 signaling pathway. Essential in hermaphrodites for formation of the vulva, uterus, and uterine seam cells and for development and maintenance of the somatic gonad and thus the germ line. Acts to prevent cep-1 from triggering an inappropriate cell cycle arrest, thereby promoting germ cell proliferation. Regulates anchor cell polarity and the timing of anchor cell invasion through the basement membranes separating vulval and somatic gonadal cells during the L3 larval stage. KEYWORDS: ATP-binding;Cytoplasm;Developmental protein;Kinase;Nucleotide-binding;Nucleus;Phosphoprotein;Reference proteome;Serine/threonine-protein kinase;Transferase SUBCELLULAR LOCATION: Nucleus Cytoplasm Nucleus, Cajal body Note=Nuclear during interphase, accumulates at the nuclear rim in prophase and localizes to chromatin throughout metaphase, anaphase and telophase. " Q19849,"PROTEIN NAMES: Transcription enhancer factor-like protein egl-44 (Egg-laying defective protein 44) ORGANISM: Caenorhabditis elegans FUNCTION: Transcription factor. Binds to DNA sequence motif 5'-CATNNNNAAATGCAT-3' as a heterodimer with egl-46. Represses expression of genes involved in differentiation of touch receptor neurons (TRN), probably acting as a heterodimer with egl-46, perhaps by occupying similar cis-regulatory elements as an unc-86/mec-3 heterodimer. Plays a role in cell fate specification of neurons, including the hook neuron HOB, and touch receptor neurons. Involved in male mating behavior, acting in concert with egl-46, via modulation of expression of polycystins lov-1 and pkd-2, homeodomain protein ceh-26, and neuropeptide-like protein nlp-8. Acts upstream of egl-46 to prevent touch cell differentiation in FLP neurons. Plays a role in neuron differentiation by repressing the expression of zag-1 in FLP neurons, probably acting as a heterodimer with egl-46; because zag-1 represses expression of egl-46 and egl-44, together these proteins form a bistable, negative-feedback loop that regulates the choice between neuronal fates. Also promotes HSN neuron development. In association with egl-46, regulates cell cycle exit in the neuronal Q cell lineage. Plays a role in specifying commissural dendrites of the PVD nociceptive neurons, acting in concert with egl-46. May be involved in thermal stress response downstream of yap-1. KEYWORDS: Alternative splicing;Cell cycle;Developmental protein;Differentiation;DNA-binding;Neurogenesis;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " Q1ECV4,"PROTEIN NAMES: Tubulin monoglycylase TTLL3 (Tubulin--tyrosine ligase-like protein 3) ORGANISM: Danio rerio (Zebrafish) (Brachydanio rerio) FUNCTION: Monoglycylase which modifies alpha- and beta-tubulin, adding a single glycine on the gamma-carboxyl groups of specific glutamate residues to generate monoglycine side chains within the C-terminal tail of tubulin. Not involved in elongation step of the polyglycylation reaction (By similarity). Preferentially glycylates a beta-tail peptide over the alpha-tail, although shifts its preference toward alpha-tail as beta-tail glutamylation increases (By similarity). Competes with polyglutamylases for modification site on beta-tubulin substrate, thereby creating an anticorrelation between glycylation and glutamylation reactions (By similarity). Not involved in elongation step of the polyglycylation reaction (By similarity). KEYWORDS: ATP-binding;Cell projection;Cilium;Cytoplasm;Cytoskeleton;Flagellum;Ligase;Magnesium;Metal-binding;Microtubule;Nucleotide-binding;Reference proteome SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton Cell projection, cilium Cytoplasm, cytoskeleton, cilium axoneme Cytoplasm, cytoskeleton, flagellum axoneme " Q1ECW2,"PROTEIN NAMES: Neuronal PAS domain-containing protein 4A (Neuronal PAS4A) ORGANISM: Danio rerio (Zebrafish) (Brachydanio rerio) FUNCTION: Transcription factor expressed in neurons of the brain that regulates the excitatory-inhibitory balance within neural circuits and is required for contextual memory in the hippocampus. Plays a key role in the structural and functional plasticity of neurons. Acts as an early-response transcription factor in both excitatory and inhibitory neurons, where it induces distinct but overlapping sets of late-response genes in these two types of neurons, allowing the synapses that form on inhibitory and excitatory neurons to be modified by neuronal activity in a manner specific to their function within a circuit, thereby facilitating appropriate circuit responses to sensory experience. KEYWORDS: Activator;Differentiation;DNA-binding;Neurogenesis;Nucleus;Reference proteome;Repeat;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " Q1ELU5,"PROTEIN NAMES: M-zodatoxin-Lt4a (M-ZDTX-Lt4a) [Cleaved into: Repetitive polypeptide element type 1a (Rpe 1a); Repetitive polypeptide element type 1b (Rpe 1b); M-zodatoxin-Lt4a peptide (Latarcin-4a) (Ltc-4a)] PROTEIN FAMILY: Cationic peptide 03 (latarcin) family, 04 subfamily ORGANISM: Lachesana tarabaevi (Spider) SIMILARITY: Belongs to the cationic peptide 03 (latarcin) family. 04 subfamily. FUNCTION: M-zodatoxin-Lt4a: Has antimicrobial activity against Gram-positive bacteria (A.globiformis VKM Ac-1112 (MIC=0.3 uM), and B.subtilis VKM B-501 (MIC=1.1 uM)), Gram-negative bacteria (E.coli DH5-alpha (MIC=4.5 uM), E.coli MH1 (MIC=3.2 uM), and P.aeruginosa PAO1 (MIC>35 uM)), and yeasts (P.pastoris GS115 (MIC=36 uM), and S.cerevisiae Y190 (MIC=18 uM)). Does not have hemolytic activity against rabbit erythrocytes. Causes paralysis, but is not lethal when injected into insect (M.domestica) larvae.; FUNCTION: [Repetitive polypeptide element type 1a]: Shows no antimicrobial activity against Gram-positive bacterium B.subtilis B-501 or Gram-negative bacterium E.coli DH5-alpha at concentrations up to 20 uM.; FUNCTION: [Repetitive polypeptide element type 1b]: Shows no antimicrobial activity against Gram-positive bacterium B.subtilis B-501 or Gram-negative bacterium E.coli DH5-alpha at concentrations up to 20 uM. Shows no toxicity towards insect (S.carnaria) larvae. KEYWORDS: Alternative splicing;Amidation;Antibiotic;Antimicrobial;Direct protein sequencing;Fungicide;Repeat;Secreted;Signal;Toxin SUBCELLULAR LOCATION: Secreted " Q1ERP8,"PROTEIN NAMES: CMRF35-like molecule 9 (CLM-9) (CD300 antigen-like family member G) (Nepmucin) (CD antigen CD300g) PROTEIN FAMILY: CD300 family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the CD300 family. FUNCTION: Receptor which may mediate L-selectin-dependent lymphocyte rollings. Binds SELL in a calcium dependent manner. Binds lymphocyte. KEYWORDS: Alternative splicing;Cell membrane;Disulfide bond;Endosome;Glycoprotein;Immunity;Immunoglobulin domain;Membrane;Receptor;Reference proteome;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Apical cell membrane ; Single-pass type I membrane protein Basolateral cell membrane ; Single-pass type I membrane protein Endosome, multivesicular body membrane ; Single-pass type I membrane protein Note=Exclusively localized on capillary endothelium. Transcytoses across the cytoplasm in the capillary endothelium. " Q1G3U6,"PROTEIN NAMES: Plant cysteine oxidase 3 (AtPCO3) (NIFS-like protein 2) (NifS2) PROTEIN FAMILY: Cysteine dioxygenase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the cysteine dioxygenase family. FUNCTION: Catalyzes the oxidation of N-terminal cysteine residues (N-Cys), thus preparing the protein for N-end rule pathway-mediated proteasomal degradation, upstream of the N-end rule enzymes ATE1, ATE2 and PRT6 (Probable). Controls the preparation of the group VII ethylene response factor (ERF-VII) proteins for degradation via the 26S proteasome N-end rule pathway (Probable). Acts as an oxygen sensor that controls the stability of ERF-VII proteins, which are stabilized in flooding-induced hypoxia, and regulate transcriptional adaptation to these adverse conditions (Probable). KEYWORDS: Alternative splicing;Cytoplasm;Iron;Metal-binding;Nucleus;Oxidoreductase;Reference proteome SUBCELLULAR LOCATION: Nucleus Cytoplasm " Q1H5E9,"PROTEIN NAMES: Protein PEP-RELATED DEVELOPMENT ARRESTED 1, chloroplastic (AtPRDA1) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Plays an essential role in early steps of chloroplast development. May be involved in the redox control of plastid gene expression by maintening the redox state around chloroplast nucleoids. May positively regulate plastid-encoded RNA polymerase (PEP) activity, through binding to FSD2. KEYWORDS: Chloroplast;Plastid;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast stroma, chloroplast nucleoid " Q1HAQ0,"PROTEIN NAMES: Lysophosphatidylcholine acyltransferase 1 (LPC acyltransferase 1) (LPCAT-1) (LysoPC acyltransferase 1) (1-acylglycerol-3-phosphate O-acyltransferase) (1-acylglycerophosphocholine O-acyltransferase) (1-alkenylglycerophosphocholine O-acyltransferase) (1-alkylglycerophosphocholine O-acetyltransferase) (Acetyl-CoA:lyso-platelet-activating factor acetyltransferase) (Acetyl-CoA:lyso-PAF acetyltransferase) (Lyso-PAF acetyltransferase) (LysoPAFAT) (Acyltransferase-like 2) PROTEIN FAMILY: 1-acyl-sn-glycerol-3-phosphate acyltransferase family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family. FUNCTION: Exhibits acyltransferase activity. Exhibits acetyltransferase activity (By similarity). Activity is calcium-independent (By similarity). Catalyzes the conversion of lysophosphatidylcholine (1-acyl-sn-glycero-3-phosphocholine or LPC) into phosphatidylcholine (1,2-diacyl-sn-glycero-3-phosphocholine or PC). Catalyzes the conversion 1-acyl-sn-glycerol-3-phosphate (lysophosphatidic acid or LPA) into 1,2-diacyl-sn-glycerol-3-phosphate (phosphatidic acid or PA) by incorporating an acyl moiety at the sn-2 position of the glycerol backbone. Displays a clear preference for saturated fatty acyl-CoAs, and 1-myristoyl or 1-palmitoyl LPC as acyl donors and acceptors, respectively (By similarity). Involved in platelet-activating factor (PAF) biosynthesis by catalyzing the conversion of the PAF precursor, 1-O-alkyl-sn-glycero-3-phosphocholine (lyso-PAF) into 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine (PAF) (By similarity). May synthesize phosphatidylcholine in pulmonary surfactant, thereby playing a pivotal role in respiratory physiology. Involved in the regulation of lipid droplet number and size (By similarity). PATHWAY: Lipid metabolism; phospholipid metabolism. KEYWORDS: Acyltransferase;Calcium;Cell membrane;Endoplasmic reticulum;Golgi apparatus;Lipid biosynthesis;Lipid droplet;Lipid metabolism;Membrane;Metal-binding;Phospholipid biosynthesis;Phospholipid metabolism;Reference proteome;Repeat;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Single-pass type II membrane protein Golgi apparatus membrane ; Single-pass type II membrane protein Cell membrane ; Single-pass type II membrane protein Lipid droplet Note=May adopt a monotopic topology when embedded in the lipid monolayer of the lipid droplet, with both termini exposed to the cytoplasm. " Q1HG43,PROTEIN NAMES: Dual oxidase maturation factor 1 (Dual oxidase activator 1) (Numb-interacting protein) PROTEIN FAMILY: DUOXA family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the DUOXA family. FUNCTION: May be required for the maturation and the transport from the endoplasmic reticulum to the plasma membrane of functional DUOX1. KEYWORDS: 3D-structure;Alternative splicing;Glycoprotein;Membrane;Protein transport;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Membrane ; Multi-pass membrane protein Q1JPL7,"PROTEIN NAMES: Pectinesterase/pectinesterase inhibitor 18 (AtPMEpcrA) [Cleaved into: Pectinesterase inhibitor 18 (Pectin methylesterase inhibitor 18); Bifunctional pectinesterase 18/rRNA N-glycosylase (PE 18) (Pectin methylesterase 18) (Pectin methylesterase 4) (AtPME4) (Ribosome-inactivating protein)] PROTEIN FAMILY: PMEI family; Pectinesterase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: In the N-terminal section; belongs to the PMEI family.; SIMILARITY: In the C-terminal section; belongs to the pectinesterase family. FUNCTION: Acts in the modification of cell walls via demethylesterification of cell wall pectin. Inhibits the elongation phase of protein synthesis. PATHWAY: Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5. KEYWORDS: Antimicrobial;Aspartyl esterase;Cell wall;Cell wall biogenesis/degradation;Direct protein sequencing;Fungicide;Hydrolase;Plant defense;Protein synthesis inhibitor;Reference proteome;Secreted;Signal;Toxin SUBCELLULAR LOCATION: Secreted, cell wall MISCELLANEOUS: The PMEI region may act as an autoinhibitory domain and prevent untimely PME activity during transport." Q1JRP2,"PROTEIN NAMES: Transmembrane protease serine 11C (Neurobin) [Cleaved into: Transmembrane protease serine 11C non-catalytic chain; Transmembrane protease serine 11C catalytic chain] PROTEIN FAMILY: Peptidase S1 family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the peptidase S1 family. FUNCTION: Serine protease which has a preference for Arg or Lys in position P1 and uncharged residues in positions P2 and P3. Shows specificity towards FGF2 in vitro. KEYWORDS: Autocatalytic cleavage;Cell membrane;Cell projection;Disulfide bond;Glycoprotein;Hydrolase;Membrane;Protease;Reference proteome;Serine protease;Signal-anchor;Transmembrane;Transmembrane helix;Zymogen SUBCELLULAR LOCATION: Cell membrane ; Single-pass type II membrane protein Cell projection, dendrite Perikaryon " Q1JUQ1,"PROTEIN NAMES: L-arabonate dehydratase (L-arabinonate dehydratase) PROTEIN FAMILY: IlvD/Edd family ORGANISM: Azospirillum brasilense SIMILARITY: Belongs to the IlvD/Edd family. FUNCTION: Catalyzes the dehydration of L-arabonate to L-2-keto-3-deoxyarabonate (L-KDA). Is involved in a degradation pathway of L-arabinose that allows A.brasilense to grow on L-arabinose as a sole carbon source. To a lesser extent, can also use D-xylonate as substrate, but not D-galactonate, D-arabonate, and D-gluconate. KEYWORDS: 4Fe-4S;Arabinose catabolism;Carbohydrate metabolism;Direct protein sequencing;Iron;Iron-sulfur;Lyase;Metal-binding " Q1K8E7,PROTEIN NAMES: GATA-type transcription factor sre (Siderophore uptake regulator sreA) ORGANISM: Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) FUNCTION: GATA-type transcription repressor that regulates iron- acquisition genes through specific binding the GATA sequence elements of target promoters in a zinc-dependent manner. Iron acquisition regulation is critical for survival under both iron-limiting conditions (to acquire essential iron) and iron-replete conditions (to limit iron toxicity). Represses the synthesis of siderophores in high iron conditions. KEYWORDS: Coiled coil;Metal-binding;Nucleus;Reference proteome;Repeat;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Q1L5Z9,PROTEIN NAMES: LON peptidase N-terminal domain and RING finger protein 2 (Neuroblastoma apoptosis-related protease) (RING finger protein 192) ORGANISM: Homo sapiens (Human) KEYWORDS: Alternative splicing;Metal-binding;Reference proteome;Repeat;TPR repeat;Zinc;Zinc-finger Q1LWH4,"PROTEIN NAMES: Fanconi-associated nuclease 1 (FANCD2/FANCI-associated nuclease 1) (Myotubularin-related protein 15) PROTEIN FAMILY: FAN1 family ORGANISM: Danio rerio (Zebrafish) (Brachydanio rerio) SIMILARITY: Belongs to the FAN1 family. FUNCTION: Nuclease required for the repair of DNA interstrand cross-links (ICL) recruited at sites of DNA damage by monoubiquitinated FANCD2. Specifically involved in repair of ICL-induced DNA breaks by being required for efficient homologous recombination, probably in the resolution of homologous recombination intermediates. Acts as a 5'-3' exonuclease that anchors at a cut end of DNA and cleaves DNA successively at every third nucleotide, allowing to excise an ICL from one strand through flanking incisions. Probably keeps excising with 3'-flap annealing until it reaches and unhooks the ICL. Acts at sites that have a 5'-terminal phosphate anchor at a nick or a 1- or 2-nucleotide flap and is augmented by a 3' flap. Also has endonuclease activity toward 5'-flaps. KEYWORDS: Coiled coil;DNA damage;DNA repair;Endonuclease;Exonuclease;Hydrolase;Magnesium;Manganese;Metal-binding;Nuclease;Nucleus;Reference proteome;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Note=Localizes at sites of DNA damage following recruitment by monoubiquitinated fancd2. " Q1LZB3,"PROTEIN NAMES: Solute carrier family 25 member 33 PROTEIN FAMILY: Mitochondrial carrier family ORGANISM: Bos taurus (Bovine) SIMILARITY: Belongs to the mitochondrial carrier (TC 2.A.29) family. FUNCTION: Mitochondrial transporter that imports/exports pyrimidine nucleotides into and from mitochondria. Selectively transports uridine, thymidine, guanosine, cytosine and inosine (deoxy)nucleoside di- and triphosphates by an antiport mechanism (By similarity). May import (deoxy)nucleoside triphosphates in exchange for intramitochondrial (deoxy)nucleoside diphosphates, thus providing precursors necessary for de novo synthesis of mitochondrial DNA and RNA while exporting products of their catabolism (By similarity). Participates in mitochondrial genome maintenance, regulation of mitochondrial membrane potential and mitochondrial respiration (By similarity). Upon INS or IGF1 stimulation regulates cell growth and proliferation by controlling mitochondrial DNA replication and transcription, the ratio of mitochondria-to nuclear-encoded components of the electron transport chain resulting in control of mitochondrial ROS production (By similarity). Participates in dendritic cell endocytosis and may associate with mitochondrial oxidative phosphorylation (By similarity). KEYWORDS: Membrane;Mitochondrion;Mitochondrion inner membrane;Reference proteome;Repeat;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Mitochondrion inner membrane ; Multi-pass membrane protein " Q1MTQ7,"PROTEIN NAMES: Kinesin-like motor protein 9 PROTEIN FAMILY: TRAFAC class myosin-kinesin ATPase superfamily, Kinesin family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. FUNCTION: Kinesin-like motor protein involved in anaphase B spindle elongation. KEYWORDS: ATP-binding;Coiled coil;Cytoplasm;Cytoskeleton;Microtubule;Motor protein;Nucleotide-binding;Nucleus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body. Cytoplasm, cytoskeleton. " Q1MX18,"PROTEIN NAMES: Protein inscuteable homolog ORGANISM: Homo sapiens (Human) FUNCTION: May function as an adapter linking the Par3 complex to the GPSM1/GPSM2 complex. Involved in spindle orientation during mitosis. May regulate cell proliferation and differentiation in the developing nervous system. May play a role in the asymmetric division of fibroblasts and participate in the process of stratification of the squamous epithelium (By similarity). KEYWORDS: 3D-structure;Alternative splicing;Cytoplasm;Developmental protein;Differentiation;Neurogenesis;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Cytoplasm, cell cortex Note=Uniformly distributed in the cytoplasm during interphase. During metaphase, detected in the cell cortex, adjacent to the mitotic spindle poles. " Q1PDP9,"PROTEIN NAMES: Transcription factor MYB115 (Myb-related protein 115) (AtMYB115) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Transcription activator that recognizes the motif 5'-TAACGG-3' in the promoter of target genes. Promotes vegetative-to-embryonic transition and the formation of somatic embryos from root explants in a WUS-independent manner. Together with MYB118, activates the transcription of S-ACP-DES2/AAD2 and S-ACP-DES3/AAD3 thus promoting the biosynthesis of omega-7 monounsaturated fatty acid in seed endosperm. KEYWORDS: Activator;DNA-binding;Nucleus;Reference proteome;Repeat;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " Q1RMT8,"PROTEIN NAMES: Interleukin-1 receptor-associated kinase 4 (IRAK-4) PROTEIN FAMILY: Protein kinase superfamily, TKL Ser/Thr protein kinase family, Pelle subfamily ORGANISM: Bos taurus (Bovine) SIMILARITY: Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily. FUNCTION: Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation to form the Myddosome together with IRAK2. Phosphorylates initially IRAK1, thus stimulating the kinase activity and intensive autophosphorylation of IRAK1. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates NCF1 and regulates NADPH oxidase activation after LPS stimulation suggesting a similar mechanism during microbial infections (By similarity). KEYWORDS: Acetylation;ATP-binding;Cytoplasm;Immunity;Innate immunity;Kinase;Magnesium;Nucleotide-binding;Phosphoprotein;Reference proteome;Serine/threonine-protein kinase;Transferase SUBCELLULAR LOCATION: Cytoplasm " Q1W3E4,"PROTEIN NAMES: L-cysteine S-thiosulfotransferase subunit SoxA (Cytochrome c550 subunit monoheme) (Protein SoxA) (SoxAX cytochrome complex subunit A) (Sulfur oxidizing protein A) (Thiosulfate-oxidizing multienzyme system protein SoxA) (TOMES protein SoxA) PROTEIN FAMILY: SoxA family ORGANISM: Allochromatium vinosum (Chromatium vinosum) SIMILARITY: Belongs to the SoxA family. FUNCTION: C-type monoheme cytochrome, which is part of the SoxAX cytochrome complex involved in sulfur oxidation. The SoxAX complex catalyzes the formation of a heterodisulfide bond between the conserved cysteine residue on a sulfur carrier SoxYZ complex subunit SoxY and thiosulfate or other inorganic sulfur substrates. This leads to the intermediary formation of conspicuous sulfur globules inside of the cells. KEYWORDS: Disulfide bond;Electron transport;Heme;Iron;Metal-binding;Periplasm;Signal;Transferase;Transport SUBCELLULAR LOCATION: Periplasm " Q20057,"PROTEIN NAMES: Enhancer of rudimentary homolog 2 PROTEIN FAMILY: E(R) family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the E(R) family. FUNCTION: Required for chromosome segregation and cell division in early embryos. Component of the pid-1 and tost-1 variants of the PETISCO complexes, which have roles in the biogenesis of a class of 21 nucleotide PIWI-interacting RNAs (piRNAs) that possess a uracil residue at the 5'-end (also called 21U-RNAs) and embryogenesis, respectively. Within the tost-1 variant of the PETISCO complex binds to splice leader SL1 RNA fragments to possibly play a role in their processing. Promotes the biogenesis of 21U-RNAs. KEYWORDS: 3D-structure;Cell cycle;Cell division;Chromosome partition;Cytoplasm;Nucleus;Reference proteome;RNA-mediated gene silencing SUBCELLULAR LOCATION: Cytoplasm Cytoplasm, perinuclear region Nucleus Note=Dispersedly distributes throughout the cytoplasm in early embryos. During early embryogenesis, localizes to the nucleus at prophase of cell division, and remains in the cytosol at interphase in 2- and 4-cell embryos. Localizes to puncta in the perinuclear region in the germline syncytium. " Q20085,"PROTEIN NAMES: Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase wee-1.1 (Myt1 kinase) PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family, WEE1 subfamily ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily. FUNCTION: Acts as a negative regulator of entry into mitosis (G2 to M transition) by phosphorylation of the CDK1 kinase. KEYWORDS: ATP-binding;Cell cycle;Developmental protein;Differentiation;Kinase;Magnesium;Metal-binding;Nucleotide-binding;Nucleus;Reference proteome;Serine/threonine-protein kinase;Transferase SUBCELLULAR LOCATION: Nucleus Note=Observed in the nucleus during prophase and metaphase. " Q20347,"PROTEIN NAMES: MAP kinase kinase mkk-4 PROTEIN FAMILY: Protein kinase superfamily, STE Ser/Thr protein kinase family, MAP kinase kinase subfamily ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily. FUNCTION: Activity is required in presynaptic neurons, in a dose-dependent manner, for normal presynaptic development and morphology. Plays a role in the formation of muscle connections, also called muscle arm extensions, between the body wall and the motor axons in the dorsal and ventral cord. KEYWORDS: ATP-binding;Cytoplasm;Kinase;Nucleotide-binding;Reference proteome;Serine/threonine-protein kinase;Transferase SUBCELLULAR LOCATION: Cytoplasm " Q20624,"PROTEIN NAMES: Molybdenum cofactor biosynthesis protein moc-5 [Includes: GTP 3',8-cyclase (Molybdenum cofactor biosynthesis protein A); Cyclic pyranopterin monophosphate synthase (Molybdenum cofactor biosynthesis protein C)] PROTEIN FAMILY: MoaC family; Radical SAM superfamily, MoaA family ORGANISM: Caenorhabditis elegans SIMILARITY: In the C-terminal section; belongs to the MoaC family.; SIMILARITY: In the N-terminal section; belongs to the radical SAM superfamily. MoaA family. FUNCTION: [Isoform a]: Probably forms a complex with isoform b that catalyzes the conversion of 5'-GTP to cyclic pyranopterin monophosphate (cPMP) (By similarity). Catalyzes the cyclization of GTP to (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate and mocs1b catalyzes the subsequent conversion of (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate to cPMP (By similarity).; FUNCTION: [Isoform b]: Probably forms a complex with isoform a that catalyzes the conversion of 5'-GTP to cyclic pyranopterin monophosphate (cPMP). PATHWAY: Cofactor biosynthesis; molybdopterin biosynthesis. KEYWORDS: 4Fe-4S;Alternative splicing;GTP-binding;Iron;Iron-sulfur;Lyase;Metal-binding;Molybdenum cofactor biosynthesis;Nucleotide-binding;Reference proteome;S-adenosyl-L-methionine " Q20646,"PROTEIN NAMES: Transcription factor cep-1 (C.elegans p53-like protein 1) PROTEIN FAMILY: P53 family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the p53 family. FUNCTION: Transcriptional activator that binds the same DNA consensus sequence as p53. Has a role in normal development to ensure proper meiotic chromosome segregation. Promotes apoptosis under conditions of cellular and genotoxic stress in response to DNA damage, hypoxia, or starvation. Regulates germline apoptosis in response to DNA damage. Its pro-apoptotic activity is inhibited when bound to ape-1 in vitro. Plays a role in cell cycle arrest in the germline in response to DNA damage by UV-C light. However, not required for survival in response to DNA damage induced by UV-C light, indicating that it is unlikely to be involved in DNA repair. Required for induction of ced-13 in response to DNA damage. Regulates DNA damage-induced apoptosis by inducing transcription of the programmed cell death activator egl-1. Regulates germline proliferation by activating phg-1. Modulates lifespan. KEYWORDS: 3D-structure;Activator;Alternative splicing;Apoptosis;DNA-binding;Metal-binding;Nucleus;Reference proteome;Transcription;Transcription regulation;Tumor suppressor;Zinc SUBCELLULAR LOCATION: Nucleus " Q20679,"PROTEIN NAMES: Multifunctional procollagen lysine hydroxylase and glycosyltransferase (Lethal protein 268) [Includes: Procollagen-lysine,2-oxoglutarate 5-dioxygenase (Lysyl hydroxylase) (LH); Procollagen glycosyltransferase (Galactosylhydroxylysine-glucosyltransferase) (Procollagen galactosyltransferase) (Procollagen glucosyltransferase)] ORGANISM: Caenorhabditis elegans FUNCTION: Multifunctional enzyme that catalyzes a series of post-translational modifications on Lys residues in procollagen. Catalyzes the formation of hydroxylysine residues in -Xaa-Lys-Gly- sequences in type IV collagens (By similarity). Transfers galactose onto hydroxylysine groups, giving rise to galactosyl 5-hydroxylysine (By similarity). Catalyzes the subsequent transfer of glucose moieties, giving rise to 1,2-glucosylgalactosyl-5-hydroxylysine residues. Essential for normal biosynthesis and secretion of type IV collagens. Essential for normal stability of the basement membrane. KEYWORDS: Dioxygenase;Disulfide bond;Endoplasmic reticulum;Glycoprotein;Glycosyltransferase;Iron;Manganese;Membrane;Metal-binding;Multifunctional enzyme;Oxidoreductase;Reference proteome;Secreted;Signal;Transferase;Vitamin C SUBCELLULAR LOCATION: Rough endoplasmic reticulum Endoplasmic reticulum lumen Endoplasmic reticulum membrane ; Peripheral membrane protein ; Lumenal side Secreted Secreted, extracellular space Note=The majority of the secreted protein is associated with the extracellular matrix. " Q20826,PROTEIN NAMES: Neuroligin-like protein glit-1 (Gliotactin homolog) (Inactive esterase glit-1) PROTEIN FAMILY: Type-B carboxylesterase/lipase family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the type-B carboxylesterase/lipase family. FUNCTION: Probable neuronal cell surface protein thought to be involved in cell-cell-interactions (Probable). Confers protection against oxidative stress. Plays a role in protecting dopaminergic neurons against oxidative stress-induced neurodegeneration. KEYWORDS: Cell membrane;Glycoprotein;Membrane;Reference proteome;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein Q20924,"PROTEIN NAMES: Sun domain-containing protein 1 ORGANISM: Caenorhabditis elegans FUNCTION: Involved in centrosome attachment to the nucleus. Required for zyg-12 localization to the nuclear envelope. Together with pot-1, it is required to anchor telomeres to the nuclear envelope in embryos. KEYWORDS: Coiled coil;Membrane;Nucleus;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Nucleus membrane ; Single-pass membrane protein Nucleus envelope " Q20929,"PROTEIN NAMES: G-protein coupled receptor dmsr-1 (Dromyosuppressin receptor related 1) PROTEIN FAMILY: G-protein coupled receptor 1 family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the G-protein coupled receptor 1 family. FUNCTION: G-protein coupled receptor.; FUNCTION: [Isoform a]: G-protein coupled receptor for flp-13 RFamide neuropeptides in vitro. Upon activation by flp-13 RFamide neuropeptides, promotes sleep in response to cellular stress also known as stress-induced sleep (SIS), probably by inhibiting the activity of wake-promoting neurons. KEYWORDS: Alternative splicing;Cell membrane;G-protein coupled receptor;Glycoprotein;Membrane;Receptor;Reference proteome;Transducer;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein " Q20977,"PROTEIN NAMES: Signal transducer and activator of transcription b (Zinc finger protein STAT-B) PROTEIN FAMILY: Transcription factor STAT family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the transcription factor STAT family. FUNCTION: Carries out a dual function: signal transduction and activation of transcription (By similarity). Required, in concert with transcription factor elt-3, for up-regulation of the vacuolar H(+)-ATPase and acceleration of lysosome maturation at molt. As part of the innate immune response to molting and injury of the adult epidermis, positively regulates the expression of epidermal antimicrobial peptides, such as nlp-29. Through positively modulating the expression of epidermal antimicrobial peptides, such as nlp-29, plays a role in resistance to fungal infection and in the response to physical wounding and phorbol ester PMA treatment. Functions cell autonomously in the epidermis, in concert with sodium-dependent transporter snf-12, probably acting at vesicular membranes, downstream of a p38 MAPK/pmk-1 pathway. KEYWORDS: Activator;Cytoplasm;Developmental protein;DNA-binding;Nucleus;Phosphoprotein;Reference proteome;SH2 domain;Transcription;Transcription regulation SUBCELLULAR LOCATION: Cytoplasm Nucleus Vesicle Note=Translocated into the nucleus in response to phosphorylation (By similarity). Localized to vesicles, which may be a type of endosome. " Q20992,"PROTEIN NAMES: Acyl-coenzyme A oxidase acox-3 PROTEIN FAMILY: Acyl-CoA oxidase family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the acyl-CoA oxidase family. FUNCTION: Involved in the first step of peroxisomal beta-oxidation by catalyzing the desaturation of fatty acid-derived side chains of ascaroside pheromones, which regulates development and behavior. Specifically, shortens indol-3-carbonyl(IC)-ascarosides with 7-carbon (IC-asc-C7) or 9-carbon (IC-asc-C9) side chains and contributes to the shortening of ascarosides with 13-carbon (asc-C13) and 15-carbon (asc-C15) side chains. PATHWAY: Lipid metabolism; peroxisomal fatty acid beta-oxidation. KEYWORDS: FAD;Fatty acid metabolism;Flavoprotein;Lipid metabolism;Oxidoreductase;Peroxisome;Reference proteome SUBCELLULAR LOCATION: Peroxisome " Q21086,PROTEIN NAMES: Guanine nucleotide-binding protein-like 3 homolog (Nucleostemin-1) PROTEIN FAMILY: TRAFAC class YlqF/YawG GTPase family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the TRAFAC class YlqF/YawG GTPase family. FUNCTION: May play a role in regulating cellular proliferation in both germline and somatic tissues. KEYWORDS: Coiled coil;GTP-binding;Nucleotide-binding;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus Q21121,"PROTEIN NAMES: Calcium uniporter protein, mitochondrial (cMCU) PROTEIN FAMILY: MCU family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the MCU (TC 1.A.77) family. FUNCTION: Mitochondrial inner membrane calcium uniporter that mediates calcium uptake into mitochondria. Constitutes a pore-forming and calcium-conducting subunit (By similarity). Mitochondrial calcium homeostasis plays key roles in cellular physiology and regulates cell bioenergetics, cytoplasmic calcium signals and activation of cell death pathways (By similarity). Required for rapid mitochondrial calcium uptake and mitochondrial reactive oxygen species (mtROS) production after wounding. In addition, together with mitochondrial calcium regulator micu-1, required for mitochondrial calcium uptake following axon injury in PLM touch receptor neurons. KEYWORDS: 3D-structure;Calcium;Calcium channel;Calcium transport;Coiled coil;Ion channel;Ion transport;Membrane;Mitochondrion;Mitochondrion inner membrane;Reference proteome;Transit peptide;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Mitochondrion inner membrane ; Multi-pass membrane protein " Q21221,"PROTEIN NAMES: Alpha/beta hydrolase domain-containing protein aho-3 (Abnormal hunger orientation protein 3) PROTEIN FAMILY: AB hydrolase superfamily, ABHD17 family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the AB hydrolase superfamily. ABHD17 family. FUNCTION: Hydrolyzes fatty acids from S-acylated cysteine residues in proteins (By similarity). Acts in sensory neurons including AWC to regulate starvation-induced thermotaxis plasticity and salt learning behavior. KEYWORDS: Cell membrane;Cytoplasmic vesicle;Hydrolase;Lipoprotein;Membrane;Palmitate;Reference proteome SUBCELLULAR LOCATION: Cell membrane ; Lipid-anchor ; Cytoplasmic side Cytoplasmic vesicle membrane ; Lipid-anchor ; Cytoplasmic side Note=In neurons, localizes to the sensory endings and to cytoplasmic punctate structures. " Q21222,"PROTEIN NAMES: ATPase family protein 2 homolog (Cell division cycle-related protein 48.3) PROTEIN FAMILY: AAA ATPase family, AFG2 subfamily ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the AAA ATPase family. AFG2 subfamily. FUNCTION: ATP-dependent chaperone which uses the energy provided by ATP hydrolysis to generate mechanical force to disassemble protein complexes (By similarity). Required for various steps of embryonic mitosis including centrosome duplication, spindle assembly, ER dynamics and cell cycle progression. Regulates the stability and activity of kinase air-2, a component of the chromosomal passenger complex (CPC). Inhibits air-2 kinase activity from metaphase to late telophase and negatively regulates air-2 stability during mitotic exit. Controls ER transition into sheet-like structures at the onset of mitosis, possibly by regulating homotypic membrane fusion. KEYWORDS: ATP-binding;Cell cycle;Cell division;Chaperone;Cytoplasm;Hydrolase;Mitosis;Nucleotide-binding;Reference proteome SUBCELLULAR LOCATION: Cytoplasm " Q21241,"PROTEIN NAMES: Leukocyte cell-derived chemotaxin-2 homolog (Chondromodulin II) PROTEIN FAMILY: LECT2/MIM-1 family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the LECT2/MIM-1 family. FUNCTION: Muscle-derived dendritic guidance cue, which is required for the formation of somatosensory dendritic arbors which extend from PVD and FLP sensory neurons during development. Ligand of a multi-protein dma-1 receptor-ligand complex, which is activated upon binding of lect-2, mnr-1 and sax-7 ligands to control the growth of dendrites that extend anteriorly from the PVD neuronal cell body. Enhances the binding of the mnr-1 and sax-7 ligands to the dma-1 receptor-ligand complex. Restricts the growth of secondary PVD dendritic branches and any irregularly positioned ectopic tertiary dendritic branches that originate from secondary branches, and promotes the formation of stable higher order dendritic branches. In particular, it is required for the formation of quaternary PVD dendritic branches and promotes their innervation of body wall muscles. Promotes self-avoidance of tertiary dendritic branches of PVD sensory neurons. Not required for the growth of dendrites that extend from AIY and PVQ interneurons, DVB GABergic neurons, PLM and ALM mechanosensory neurons, AFD sensory neurons and DD/VD and DA/DB motor neurons. KEYWORDS: Basement membrane;Cell junction;Cell projection;Disulfide bond;Extracellular matrix;Glycoprotein;Metal-binding;Neurogenesis;Reference proteome;Secreted;Signal;Zinc SUBCELLULAR LOCATION: Secreted Cell junction Secreted, extracellular space, extracellular matrix, basement membrane Cell projection, dendrite Perikaryon Cell surface Note=Secreted by body wall muscles and taken up by coelomocytes. Localizes to the basement membrane of hypodermal tissues, body wall muscles and along fibrous organelles in larvae and adults. Co-localizes with sax-7 at dendrites. Dendritic localization is dependent on sax-7, but not mnr-1 or dma-1. " Q21734,"PROTEIN NAMES: Putative ribosomal protein S6 kinase alpha-1 PROTEIN FAMILY: Protein kinase superfamily, AGC Ser/Thr protein kinase family, S6 kinase subfamily ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily. FUNCTION: Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of transcription (By similarity). Suppresses germline tumor formation by preventing the dedifferentiation of secondary spermatocytes probably downstream of mpk-1. KEYWORDS: Alternative splicing;ATP-binding;Kinase;Nucleotide-binding;Phosphoprotein;Reference proteome;Repeat;Serine/threonine-protein kinase;Transferase " Q21738,"PROTEIN NAMES: Partner of xrn-2 protein 1 ORGANISM: Caenorhabditis elegans FUNCTION: Plays a role in maintenance of steady-state concentration and turnover of microRNAs (miRNA) by degradation of mature miRNA in complex with the exoribonuclease xrn-2. Stabilizes and enhances the accumulation and activity of the exoribonuclease xrn-2, and thus contributes to miRNA turnover. KEYWORDS: 3D-structure;mRNA processing;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus, nucleolus Nucleus, nucleoplasm " Q21921,"PROTEIN NAMES: NAD-dependent protein deacetylase sir-2.1 (Protein sir-2.1) (Regulatory protein SIR2 homolog 1) PROTEIN FAMILY: Sirtuin family, Class I subfamily ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the sirtuin family. Class I subfamily. FUNCTION: NAD-dependent deacetylase (By similarity). Involved in metabolism, apoptosis, response to oxidative stress, response to DNA damage, and determination of lifespan. Required for a reduction of the 'Lys-16' acetylation of histone H4 (H4K16ac) on dosage-compensated X chromosomes in hermaphrodites. Plays a role in germ cell and somatic cell apoptosis in response to DNA damage. Functions upstream of daf-16/Forkhead box protein O in the Insulin/IGF-1-like signaling (IIS) mediated pathway, promoting daf-16 mediated transcriptional activation and increased lifespan. May also regulate lifespan independently of daf-16 by modulating the transcription of genes involved in the stress response of the endoplasmic reticulum (ER). Functions upstream of transcriptional coregulator hcf-1, perhaps acting independently of the IIS mediated pathway, to modulate lifespan and oxidative stress response. Acts upstream of the nicotinic acid metabolism pathway, which may be linked to the regulation of longevity. Plays a role in ascaroside-mediated longevity and stress resistance. KEYWORDS: Cytoplasm;Metal-binding;NAD;Nucleus;Reference proteome;Transferase;Zinc SUBCELLULAR LOCATION: Nucleus Cytoplasm Note=Translocates from the nucleus to the cytoplasm during apoptosis. " Q21966,"PROTEIN NAMES: Aspartic protease 4 PROTEIN FAMILY: Peptidase A1 family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the peptidase A1 family. FUNCTION: Aspartic protease, which is part of the necrosis cell death pathway. Involved in neuronal cell degeneration. Involved in heat stress response. KEYWORDS: Aspartyl protease;Cytoplasm;Disulfide bond;Glycoprotein;Hydrolase;Lysosome;Necrosis;Protease;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Cytoplasm Lysosome Secreted " Q22036,"PROTEIN NAMES: Calpain-5 (Sex-determining transformer protein 3) PROTEIN FAMILY: Peptidase C2 family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the peptidase C2 family. FUNCTION: Required for the correct female sexual development of the soma and germline in hermaphrodite animals, while being fully dispensable in males. Has calcium-dependent proteolytic activity and is involved in the cleavage of tra-2, for which it acts as a potentiator. Capable of calcium-dependent autolysis. Part of the necrosis cell death pathway. Required for necrosis of intestinal cells induced by B.thuringiensis endotoxin Cry6Aa. KEYWORDS: Autocatalytic cleavage;Calcium;Differentiation;Hydrolase;Necrosis;Protease;Reference proteome;Sexual differentiation;Thiol protease " Q22053,"PROTEIN NAMES: rRNA 2'-O-methyltransferase fibrillarin (Histone-glutamine methyltransferase) PROTEIN FAMILY: Methyltransferase superfamily, Fibrillarin family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the methyltransferase superfamily. Fibrillarin family. FUNCTION: S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins (By similarity). Involved in pre-rRNA processing. Utilizes the methyl donor S-adenosyl-L-methionine to catalyze the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA (By similarity). Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA (By similarity). Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ105me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus (By similarity). Plays a role in modulation of nucleolus size most likely through regulating the ribosomal RNA (rRNA) pool. KEYWORDS: Methylation;Methyltransferase;Nucleus;Reference proteome;Ribonucleoprotein;RNA-binding;rRNA processing;S-adenosyl-L-methionine;Transferase SUBCELLULAR LOCATION: Nucleus, nucleolus Nucleus, nucleoplasm Note=Fibrillar region of the nucleolus. Predominantly localizes to the nucleolus, but also localizes to small punctate structures throughout the nucleoplasm. Co-localizes with dao-5 in nucleoli. " Q22397,"PROTEIN NAMES: Amino acid transporter protein 6 PROTEIN FAMILY: Amino acid-polyamine-organocation (APC) superfamily ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the amino acid-polyamine-organocation (APC) superfamily. FUNCTION: Amino acid transporter that mediates the uptake of the L-enantiomers of various amino acids, including L-glutamate (Probable). May play a role in promoting fertility. KEYWORDS: Alternative splicing;Amino-acid transport;Cell membrane;Glycoprotein;Membrane;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Apical cell membrane ; Multi-pass membrane protein Note=Co-localizes with nrfl-1 at the apical cell membrane of intestinal cells. " Q22495,"PROTEIN NAMES: Tetraspanin-12 PROTEIN FAMILY: Tetraspanin (TM4SF) family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the tetraspanin (TM4SF) family. FUNCTION: Functions redundantly with tsp-14 isoform a to regulate body size, embryonic and vulva development. Functions redundantly with tsp-14 (isoforms a and b) to regulate cell fate specification in the postembryonic mesodermal M lineage and male development. May regulate BMP-like Sma/Mab signaling by mediating protease sup-17 trafficking to the cell surface. Together with tsp-14, functions redundantly to maintain cell surface levels of the BMP type II receptor daf-4 (but not BMP type I receptor sma-6), probably by regulating endosomal sorting of receptors and their targeting to degradative lysosomes. Together with tsp-14 involved in maintaining the structural and functional integrity of the endosomal network. Together with tsp-14, probably acts by modulating the activation of glp-1, a Notch-like receptor, to regulate germline maturation. Probably acts by modulating the activation of lin-12, a Notch-like receptor, to regulate cell fate specification such as the anchor cell/ventral uterine precursor cell decision. KEYWORDS: Cell membrane;Cytoplasmic vesicle;Endosome;Glycoprotein;Golgi apparatus;Membrane;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Cytoplasmic vesicle membrane ; Multi-pass membrane protein Endosome membrane ; Multi-pass membrane protein Early endosome membrane ; Multi-pass membrane protein Late endosome membrane ; Multi-pass membrane protein Recycling endosome membrane ; Multi-pass membrane protein Golgi apparatus, trans-Golgi network membrane; Multi-pass membrane protein Note=Often in close juxtaposition to the retromer but not part of it, suggesting separate microdomains of the same endosome. " Q22557,"PROTEIN NAMES: DNA-dependent metalloprotease dvc-1 (DNA damage protein targeting VCP) (DVC1) (Protein with SprT-like domain at the N terminus) (Spartan) PROTEIN FAMILY: Spartan family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the Spartan family. FUNCTION: DNA-dependent metalloendopeptidase that mediates the proteolytic cleavage of covalent DNA-protein cross-links (DPCs) during DNA synthesis, thereby playing a key role in maintaining genomic integrity. DPCs are highly toxic DNA lesions that interfere with essential chromatin transactions, such as replication and transcription, and which are induced by reactive agents, such as UV light or formaldehyde. Associates with the DNA replication machinery and specifically removes DPCs during DNA synthesis. Regulator of UV-induced DNA damage response: required to protect genome stability during DNA replication, possibly via recruitment of vcp/p97 (cdc-48.1 or cdc-48.2) recruitment. KEYWORDS: Chromosome;DNA damage;DNA repair;Hydrolase;Metal-binding;Metalloprotease;Nucleus;Protease;Reference proteome;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Chromosome Note=Localizes to nuclear foci following UV treatment. " Q22592,"PROTEIN NAMES: Beclin homolog PROTEIN FAMILY: Beclin family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the beclin family. FUNCTION: Regulates autophagy. Together with phosphatidyl-3-phosphate kinase vps-34, acts as a core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate (PtdIns3P), thereby regulating membrane trafficking. In association with sorf-1 and sorf-2, negatively regulates phosphatidylinositol 3-phosphate in early endosomes to allow for the conversion to late endosomes. Involved in the clearance of engulfed apoptotic cell corpses. Together with ced-9, negatively regulates somatic and germline apoptosis. Plays a role in endosome-to-Golgi retrograde transport of mig-14. In a daf-18/PTEN- and skn-1/Nrf-dependent manner, promotes germline stem cell proliferation during late and adult stages, probably by ensuring cell survival and cell cycle progression. Required for embryonic development and L3/L4 molting during larval development. Required for normal dauer morphogenesis and lifespan. Plays a role in male tail ray pattern formation. Required for normal survival when exposed to pathogenic bacteria S.typhimurium by promoting autophagic degradation of intracellular S.typhimurium. KEYWORDS: Alternative splicing;Autophagy;Cell projection;Coiled coil;Cytoplasm;Endocytosis;Endosome;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Perikaryon Cell projection, dendrite Cell projection, axon Endosome Note=Co-localizes with rme-8, a component of the retromer complex, on endosomes. " Q22618,"PROTEIN NAMES: Double-stranded RNA-specific adenosine deaminase adr-2 (DRADA) ORGANISM: Caenorhabditis elegans FUNCTION: Catalyzes the hydrolytic deamination of adenosine to inosine in double-stranded RNA (dsRNA) referred to as A-to-I RNA editing. Acts primarily on non-coding regions of protein-coding genes including introns and untranslated regions (UTR). Required for normal chemotaxis. Edits the 3' UTR of clec-41 which is required for normal clec-41 expression and for chemotaxis. Plays a role in determining lifespan. Not required for RNA interference. Likely to play a role in determining whether a dsRNA enters the RNAi pathway. KEYWORDS: Chemotaxis;Cytoplasm;Hydrolase;Metal-binding;mRNA processing;Nucleus;Reference proteome;RNA-binding;Zinc SUBCELLULAR LOCATION: Nucleus Cytoplasm MISCELLANEOUS: Can prevent somatic transgenes from inducing gene silencing via the RNA interference (RNAi) pathway. This may occur due to A-to-I editing of transgene-derived dsRNA, preventing transgene RNAi." Q22663,PROTEIN NAMES: Globin-like protein 26 PROTEIN FAMILY: Globin family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the globin family. FUNCTION: Plays a role in electron transport. Utilizes the bis-histidyl hexacoordinated complex with iron to transfer electrons to cytochrome c and molecular oxygen. Plays a regulatory role in the periodicity of the defecation cycle under oxidative stress conditions. Not involved in imparting protection against general conditions of oxidative stress. May participate in redox reactions under anaerobic conditions. KEYWORDS: Cell membrane;Cytoplasm;Heme;Iron;Lipoprotein;Membrane;Metal-binding;Myristate;Nucleus;Oxygen transport;Reference proteome;Transport SUBCELLULAR LOCATION: Cytoplasm Nucleus lamina Cell membrane Note=Transported to the nucleus by myristoylation of the N-terminal glycine. MISCELLANEOUS: Binds carbon monoxide (CO). Q22695,"PROTEIN NAMES: Cyclin-dependent kinase 5 activator 1 (p35) PROTEIN FAMILY: Cyclin-dependent kinase 5 activator family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the cyclin-dependent kinase 5 activator family. FUNCTION: Activator of the kinase cdk-5 (By similarity). In several motor neurons, promotes the polarized trafficking of synaptic vesicles and dense-core vesicles. In the ventral nerve cord, regulates the synaptic localization of the glutamate receptor, glr-1. In DA motor neurons, regulates axonal transport of synaptic vesicle precursors by inhibiting dynein-mediated retrograde transport. Regulates the polarized distribution of dense-core vesicles in DB motor neurons. May regulate these processes in association with cdk-5. May also play a role in GABAergic synaptic vesicle localization in the ventral nerve cord. KEYWORDS: Alternative splicing;Cell projection;Cytoplasm;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Cell projection, dendrite Cell projection, axon Note=Presynaptic localization in the axon and punctate localization in the dendrite. " Q22795,"PROTEIN NAMES: Histone-lysine N-methyltransferase set-1 PROTEIN FAMILY: Class V-like SAM-binding methyltransferase superfamily, Histone-lysine methyltransferase family, PR/SET subfamily ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. PR/SET subfamily. FUNCTION: Histone methyltransferase that specifically monomethylates 'Lys-20' of histone H4 (H4K20me1). H4K20me1 is enriched on hermaphrodite X chromosomes and during mitosis. Involved in dosage compensation by repression of X-linked gene expression in hermaphrodites. Plays a role in growth and body fat regulation downstream of the TOR complex 2 pathway. KEYWORDS: Chromatin regulator;Chromosome;Methyltransferase;Nucleus;Reference proteome;S-adenosyl-L-methionine;Transcription;Transcription regulation;Transferase SUBCELLULAR LOCATION: Nucleus Chromosome " Q23023,"PROTEIN NAMES: Serine/threonine-protein kinase unc-51 (Uncoordinated protein 51) PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family, APG1/unc-51/ULK1 subfamily ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily. FUNCTION: Protein kinase important for axonal elongation and axonal guidance. Functions in the CAN axons to direct both anterior and posterior migrations. Phosphorylates both unc-14 and vab-8. Component of the unc-51/atg-13 complex that is probably recruited by lgg-1 to preautophagosomes and is required for autophagosome formation. Interaction with autophagy related proteins such as atg-13 links it to the autophagy machinery to in turn promote P-granule degradation in somatic cells. Plays a role in mitophagy during limited food availability. Regulates cell size. Plays a role in male tail ray pattern formation. May be required for normal dauer morphogenesis. KEYWORDS: 3D-structure;ATP-binding;Developmental protein;Differentiation;Kinase;Magnesium;Metal-binding;Neurogenesis;Nucleotide-binding;Reference proteome;Serine/threonine-protein kinase;Transferase " Q23049,"PROTEIN NAMES: Tetratricopeptide repeat protein 8 (Bardet-Biedl syndrome 8 protein homolog) ORGANISM: Caenorhabditis elegans FUNCTION: Component of the BBSome complex (By similarity). The BBSome complex is thought to function as a coat complex required for sorting of specific membrane proteins to the primary cilia (By similarity). The BBSome complex is required for ciliogenesis but is dispensable for centriolar satellite function (By similarity). Required for proper BBSome complex assembly and its ciliary localization. Required for cilia biogenesis and both the assembly and movement of intraflagellar transport proteins along the ciliary axoneme. Plays a role in guanylyl cyclase localization in the ring-like structures at the base of the finger compartment in AFD sensory neurons. KEYWORDS: Cell projection;Cilium;Cilium biogenesis/degradation;Cytoplasm;Cytoskeleton;Protein transport;Reference proteome;Repeat;TPR repeat;Transport SUBCELLULAR LOCATION: Cell projection, cilium Cytoplasm, cytoskeleton, cilium basal body Cytoplasm, cytoskeleton, cilium axoneme Note=Localized at the ciliary base and also in ring-like structures between the base of the AFD sensory neuron finger compartment and the dendritic membrane. " Q230X8,"PROTEIN NAMES: Small RNA 2'-O-methyltransferase (HEN1 methyltransferase homolog 1) PROTEIN FAMILY: Methyltransferase superfamily, HEN1 family ORGANISM: Tetrahymena thermophila (strain SB210) SIMILARITY: Belongs to the methyltransferase superfamily. HEN1 family. FUNCTION: Methyltransferase that adds a 2'-O-methyl group at the 3'-end of piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. This probably protects the 3'-end of piRNAs from uridylation activity and subsequent degradation. Required for programmed DNA elimination. KEYWORDS: Magnesium;Metal-binding;Methyltransferase;Nucleus;Reference proteome;RNA-binding;RNA-mediated gene silencing;S-adenosyl-L-methionine;Transferase SUBCELLULAR LOCATION: Nucleus Note=Localizes to the macronucleus. " Q23175,PROTEIN NAMES: Homeobox protein ceh-32 PROTEIN FAMILY: SIX/Sine oculis homeobox family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the SIX/Sine oculis homeobox family. FUNCTION: Transcription factor which binds a motif with the core sequence 5'-GTATCA-3'. Plays a role in head morphogenesis. Involved in embryonic development. Required for cell specification of the RIA interneurons. May cooperate with the transcription factor vab-3 and phosphatase eya-1 to repress transcription factor ets-5 expression in non BAG neuronal cells. KEYWORDS: Developmental protein;DNA-binding;Homeobox;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus Q23229,"PROTEIN NAMES: XO lethal protein 1 PROTEIN FAMILY: GHMP kinase family, Xol-1 subfamily ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the GHMP kinase family. Xol-1 subfamily. FUNCTION: Sex-determining factor that is required for sexual differentiation and X chromosome dosage compensation to promote male development. High expression during gastrulation triggers male development, while low expression at that time triggers hermaphrodite development. Although related to GHMP kinase, its mode of action remains unclear. KEYWORDS: 3D-structure;Alternative splicing;Developmental protein;Differentiation;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: [Isoform b]: Only functional isoform. Produced by alternative splicing of intron 6 which results in the deletion of intron 6 and retention of exon 7 coding sequences.; MISCELLANEOUS: [Isoform c]: Inactive isoform. Produced by alternative splicing of intron 6 which results in the retention of intron 6 and deletion of exon 7 coding sequences. An in-frame UAA stop codon within intron 6 prematurely terminates translation. Intron 6 retention and exon 7 deletion can also be due to use of an alternative 3' splice acceptor site in the 3'UTR resulting in trans-splicing to the transcripts of unrelated genes." Q23255,"PROTEIN NAMES: Double-strand break repair protein mre-11 PROTEIN FAMILY: MRE11/RAD32 family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the MRE11/RAD32 family. FUNCTION: Core component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The MRN complex is involved in the repair of DNA double-strand breaks (DSBs) via homologous recombination (HR), an error-free mechanism which primarily occurs during S and G2 phases. The complex (1) mediates the end resection of damaged DNA, which generates proper single-stranded DNA, a key initial steps in HR, and is (2) required for the recruitment of other repair factors and efficient activation of ATM and ATR upon DNA damage (By similarity). Within the MRN complex, mre-11 possesses both single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity (By similarity). Mre-11 first endonucleolytically cleaves the 5' strand at DNA DSB ends to prevent non-homologous end joining (NHEJ) and licence HR (By similarity). It then generates a single-stranded DNA gap via 3' to 5' exonucleolytic degradation, which is required for single-strand invasion and recombination (By similarity). Required for meiotic crossing over and chiasma formation. Pachytene morphology and homolog pairing are normal. Vital in long term for maintenance of reproductive capacity of subsequent generations. KEYWORDS: Chromosome;DNA damage;DNA repair;Endonuclease;Exonuclease;Hydrolase;Manganese;Meiosis;Metal-binding;Nuclease;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus Chromosome Note=Localizes to DNA double-strand breaks (DSBs). " Q23272,"PROTEIN NAMES: Stress activated transcription factor atfs-1 PROTEIN FAMILY: BZIP family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the bZIP family. FUNCTION: Acts as a transcription factor during mitochondrial stress by activating the mitochondrial unfolded protein response (mtUPR). Induces nuclear and mitochondrial gene transcription, including genes coding for mitochondrial chaperones and proteins involved in glycolysis, amino acid catabolism and innate immunity. Following mitochondrial stress, restores mitochondrial respiratory capacity by limiting the transcription of oxidative phosphorylation (OXPHOS) machinery genes and by promoting the assembly of OXPHOS complexes via the up-regulation of chaperone and assembly factor genes. Component of a feedback loop involving atfs-1, atgl-1 and hlh-11. Acts together with flp-7 to negatively regulate the expression of the transcription regulator hlh-11, to promote expression of atgl-1, and thus atgl-1-dependent fat oxidation in response to mitochondrial stress. In addition, functions with hlh-11 to maintain lifespan. Promotes mtDNA maintenance and propagation of deleterious mtDNA. KEYWORDS: Alternative splicing;Cytoplasm;DNA-binding;Immunity;Innate immunity;Isopeptide bond;Mitochondrion;Nucleus;Reference proteome;Transcription;Transcription regulation;Transit peptide;Unfolded protein response SUBCELLULAR LOCATION: Mitochondrion matrix Cytoplasm Nucleus Note=In absence of mitochondrial stress, localizes mainly to mitochondria where it is rapidly degraded by protease lonp-1. Upon mitochondrial stress which causes a reduction in protein mitochondrial import, accumulates in the cytoplasm and translocates into the nucleus to activate transcription of mitochondrial unfolded protein response genes. Similarly, accumulates in the nucleus in response to P.aeruginosa-mediated infection. " Q23288,"PROTEIN NAMES: Spermatogenesis-defective protein 39 (SPE-39) (VPS33B-interacting protein in polarity and apical restriction) PROTEIN FAMILY: SPE39 family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the SPE39 family. FUNCTION: Proposed to be involved in endosomal maturation implicating in part vps-33.2. May play a role in epithelial polarization through stabilization of apical membrane protein content. May play a role in transcriptional regulation (By similarity). Plays a role in lysosomal trafficking, probably via association with the core HOPS complex in a discrete population of endosomes. Essential for vesicular trafficking during spermatogenesis; required during spermiogenesis or sperm activation for the morphogenesis of specialized Golgi-derived fibrous body-membranous organelle (FB-MO) complexes. Required for the processing of internalized proteins in oocytes and coelomocytes. KEYWORDS: Cytoplasm;Cytoplasmic vesicle;Differentiation;Endosome;Protein transport;Reference proteome;Spermatogenesis;Transcription;Transcription regulation;Transport SUBCELLULAR LOCATION: Cytoplasm Cytoplasmic vesicle Early endosome Recycling endosome Late endosome Note=Localizes in both residual bodies and budding spermatids. " Q23544,"PROTEIN NAMES: Protein adenylyltransferase fic-1 (De-AMPylase fic-1) PROTEIN FAMILY: Fic family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the fic family. FUNCTION: Protein that can both mediate the addition of adenosine 5'-monophosphate (AMP) to specific residues of target proteins (AMPylation), and the removal of the same modification from target proteins (de-AMPylation), depending on the context (By similarity). The side chain of Glu-274 determines which of the two opposing activities (AMPylase or de-AMPylase) will take place (By similarity). Adenylyltransferase that mediates the addition of adenosine 5'-monophosphate (AMP) to specific residues of target proteins. In vivo target proteins include the heat-shock 70 family proteins hsp-1 and hsp-3 and the translation elongation factors eef-1A, eef-1G and eef-2. Can AMPylate core histone H3 in vitro. Can also act as a phosphodiesterase by mediating removal of ATP (de-AMPylation) from target proteins (By similarity). Decreases susceptibility to P.aeruginosa-mediated killing and might therefore play a role in the innate immune response. KEYWORDS: 3D-structure;ATP-binding;Endoplasmic reticulum;Hydrolase;Membrane;Nucleotide-binding;Nucleotidyltransferase;Nucleus;Phosphoprotein;Reference proteome;Repeat;TPR repeat;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Single-pass membrane protein. Nucleus membrane ; Single-pass membrane protein. Note=Predominantly localized to the endoplasmic reticulum and to the nucleus. " Q23552,"PROTEIN NAMES: Phosphoethanolamine N-methyltransferase 1 (PEAMT-1) (PMT-1) (Methyltransf_25 domain-containing protein) (S-adenosyl-L-methionine:phosphoethanolamine N-methyltransferase) PROTEIN FAMILY: Class I-like SAM-binding methyltransferase superfamily ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase superfamily. FUNCTION: Catalyzes the first step in the synthesis of phosphocholine by converting phosphoethanolamine into phospho-monomethylethanolamine (N-methylethanolamine phosphate). Phosphocholine is a precursor for phosphatidylcholine, a major component in membranes and a precursor itself in the production of glycoconjugates secreted by parasitic nematodes to avoid host immune responses. PATHWAY: Phospholipid metabolism; phosphatidylcholine biosynthesis; phosphocholine from phosphoethanolamine. KEYWORDS: Alternative splicing;Lipid biosynthesis;Lipid metabolism;Phospholipid biosynthesis;Phospholipid metabolism;Reference proteome;S-adenosyl-L-methionine;Transferase " Q23588,PROTEIN NAMES: Uridine and thymidine phosphorylase (Pyrimidine-nucleoside phosphorylase) PROTEIN FAMILY: PNP/UDP phosphorylase family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the PNP/UDP phosphorylase family. FUNCTION: Catalyzes the reversible phosphorylytic cleavage of uridine and thymidine to uracil and ribose-phosphate or thymine and deoxyribose-1-phosphate. The produced molecules are then utilized as carbon and energy sources or in the rescue of pyrimidine bases for nucleotide synthesis (Probable). Required for normal lifespan. PATHWAY: Pyrimidine metabolism; UMP biosynthesis via salvage pathway; uracil from uridine (phosphorylase route): step 1/1.; PATHWAY: Pyrimidine metabolism; dTMP biosynthesis via salvage pathway; dTMP from thymine: step 1/2. KEYWORDS: Glycosyltransferase;Pyrimidine biosynthesis;Reference proteome;Transferase Q23997,"PROTEIN NAMES: Imaginal disk growth factor 6 PROTEIN FAMILY: Glycosyl hydrolase 18 family, IDGF subfamily ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the glycosyl hydrolase 18 family. IDGF subfamily. FUNCTION: Probably required to stimulate the proliferation, polarization and motility of imaginal disk cells. May act by stabilizing the binding of insulin-like peptides to its receptor through a simultaneous interaction with both molecules to form a multiprotein signaling complex (By similarity). KEYWORDS: Chitin-binding;Developmental protein;Direct protein sequencing;Disulfide bond;Glycoprotein;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted Note=It is transported to target tissues via hemolymph. " Q23TC2,"PROTEIN NAMES: Tubulin glycylase 3A ORGANISM: Tetrahymena thermophila (strain SB210) FUNCTION: Monoglycylase which modifies alpha- and beta-tubulin, generating side chains of glycine on the gamma-carboxyl groups of specific glutamate residues within the C-terminal tail of alpha- and beta-tubulin. Involved in the side-chain initiation step of the glycylation reaction by adding a single glycine chain to generate monoglycine side chains. Not involved in elongation step of the polyglycylation reaction. KEYWORDS: ATP-binding;Cell projection;Cilium;Cytoplasm;Cytoskeleton;Ligase;Nucleotide-binding;Reference proteome SUBCELLULAR LOCATION: Cell projection, cilium Cytoplasm, cytoskeleton, cilium axoneme Note=Mainly present in locomotory and oral cilia. " Q24009,PROTEIN NAMES: Protein bicaudal C PROTEIN FAMILY: BicC family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the BicC family. FUNCTION: RNA-binding protein that is involved in oogenesis. Required for correct targeting of the migrating anterior follicle cells and the establishment of anterior-posterior polarity in the oocyte. May act as translational repressor of oskar during oogenesis. Function seems to be sensitive to small changes in expression. KEYWORDS: Developmental protein;Differentiation;Oogenesis;Phosphoprotein;Reference proteome;Repeat;RNA-binding Q24046,"PROTEIN NAMES: Sodium/potassium-transporting ATPase subunit beta-1 (Protein nervana 1) (Sodium/potassium-dependent ATPase subunit beta-1) PROTEIN FAMILY: X(+)/potassium ATPases subunit beta family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the X(+)/potassium ATPases subunit beta family. FUNCTION: This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. KEYWORDS: Cell membrane;Disulfide bond;Glycoprotein;Ion transport;Membrane;Potassium;Potassium transport;Reference proteome;Signal-anchor;Sodium;Sodium transport;Sodium/potassium transport;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane ; Single-pass type II membrane protein " Q24087,"PROTEIN NAMES: DNA repair endonuclease XPF (Protein meiotic 9) PROTEIN FAMILY: XPF family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the XPF family. FUNCTION: Implicated in recombination events during meiosis, mostly in meiotic exchange. May directly resolve Holliday junctions within recombination intermediates leading to DNA exchange. Also required for the repair of mismatches within meiotic heteroduplex DNA and for nucleotide excision repair. KEYWORDS: DNA damage;DNA repair;DNA-binding;Endonuclease;Hydrolase;Meiosis;Nuclease;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus " Q24145,"PROTEIN NAMES: Tyrosine-protein kinase Shark PROTEIN FAMILY: Protein kinase superfamily, Tyr protein kinase family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. FUNCTION: Following axon injury, required for recruitment of drpr and glial cells to severed axons and for glial clearance of severed axons from the central nervous system. Together with Src42a and drpr, promotes the migration of macrophages to sites of wounding as part of a signaling cascade where Scr42a detects production of hydrogen peroxide at wound sites which triggers phosphorylation of drpr and subsequent recruitment and activation of shark. May be involved in signal transduction on the apical surface of ectodermal epithelial cells, regulating their polarity during invagination. Crumbs (crb) may be the intracellular signal. KEYWORDS: ANK repeat;ATP-binding;Cytoplasm;Kinase;Nucleotide-binding;Phosphoprotein;Reference proteome;Repeat;SH2 domain;Transferase;Tyrosine-protein kinase SUBCELLULAR LOCATION: Cytoplasm Note=Apical expression in cephalic furrow and tracheal cells. Limited to luminal surface and absent from the basal surface. " Q24151,PROTEIN NAMES: Signal transducer and transcription activator (d-STAT) (Protein marelle) PROTEIN FAMILY: Transcription factor STAT family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the transcription factor STAT family. FUNCTION: Might play a role in signal transduction and activation of transcription. Plays an important role in the segmental pattern formation in the early embryo by activating specific stripes of pair rule gene expression in early development as part of the Janus kinase-STAT pathway. Might play a role in male germline stem cell maintenance. KEYWORDS: Activator;Alternative splicing;Cytoplasm;Developmental protein;DNA-binding;Nucleus;Phosphoprotein;Reference proteome;SH2 domain;Transcription;Transcription regulation SUBCELLULAR LOCATION: Cytoplasm Nucleus Note=Translocated into the nucleus in response to phosphorylation. Q24157,"PROTEIN NAMES: Beta-1,3-galactosyltransferase brn (Brainiac protein) (Neurogenic secreted-signaling protein brn) PROTEIN FAMILY: Glycosyltransferase 31 family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the glycosyltransferase 31 family. FUNCTION: Neurogenic protein essential for the development and maintenance of epithelial structure. Required in the germline for establishing the follicular epithelium and for determining the dorsal-ventral polarity. Collaborates with Notch on the apical surface of follicle cells to mediate germline-follicle cell adhesion. Brn has a role in chorion formation. KEYWORDS: Developmental protein;Glycoprotein;Glycosyltransferase;Golgi apparatus;Membrane;Notch signaling pathway;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus membrane ; Single-pass type II membrane protein " Q24168,"PROTEIN NAMES: Origin recognition complex subunit 2 (DmORC2) PROTEIN FAMILY: ORC2 family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the ORC2 family. FUNCTION: Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent, however specific DNA sequences that define origins of replication have not been identified so far. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication. As part of the ORC complex, might also have a role in mRNA export (Probable). KEYWORDS: 3D-structure;Centromere;Chromosome;DNA replication;Nucleus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Nucleus Chromosome Chromosome, centromere Note=Centromeres of metaphase and anaphase chromosomes of early syncytial embryos. Later in development, found in pericentric regions of late anaphase and telophase chromosomes. " Q24174,"PROTEIN NAMES: Protein abrupt (Protein clueless) ORGANISM: Drosophila melanogaster (Fruit fly) FUNCTION: Expression is vital for development; may be involved in transcriptional regulation. In embryos, muscle specific expression is required for segmental nerve b (SNb) motoneuron target recognition within ventral longitudinal muscles. Has a role in establishing and maintaining embryonic muscle attachments, adult sensory cell formation (macrochaetae) and morphogenesis of adult appendages (legs, antenna aristae and male external genitalia). Has a role in the morphogenesis of the class I dendritic neurons: selective expression of ab in class I da neurons plays a pivotal role in forming dendritic arbors, which are characteristic of the class I cells. The development of more complex arbors of class II-IV neurons depends on the absence of ab. KEYWORDS: Alternative splicing;Developmental protein;DNA-binding;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Repeat;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus. " Q24206,PROTEIN NAMES: Broad-complex core protein isoform 6 ORGANISM: Drosophila melanogaster (Fruit fly) FUNCTION: Broad-complex proteins are required for puffing and transcription of salivary gland late genes during metamorphosis. KEYWORDS: Alternative splicing;Developmental protein;DNA-binding;Metal-binding;Nucleus;Reference proteome;Repeat;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus. Q24238,"PROTEIN NAMES: Alkaline phosphatase 4 PROTEIN FAMILY: Alkaline phosphatase family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the alkaline phosphatase family. FUNCTION: Important role in neural and renal epithelial function. KEYWORDS: Alternative splicing;Cell membrane;Disulfide bond;Glycoprotein;GPI-anchor;Hydrolase;Lipoprotein;Magnesium;Membrane;Metal-binding;Phosphoprotein;Reference proteome;Signal;Transmembrane;Transmembrane helix;Zinc SUBCELLULAR LOCATION: Cell membrane ; Lipid-anchor, GPI-anchor " Q24256,"PROTEIN NAMES: Homeobox protein B-H2 (Homeobox protein BarH2) PROTEIN FAMILY: Antp homeobox family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the Antp homeobox family. FUNCTION: B-H1 and B-H2 are regulated by members of the wg signaling pathway; wg and dpp. B-H1 and B-H2 are coexpressed and functionally required in R1 and R6 receptor cells and primary pigment cells for normal eye development. Coexpression is also required for the fate determination of external sensory organs, formation of notal microchaetae, formation of presutural macrochaetae, antennal development and for distal leg morphogenesis; segmentation and specification of tarsal segments 3-5. KEYWORDS: Developmental protein;DNA-binding;Homeobox;Nucleus;Phosphoprotein;Reference proteome;Sensory transduction;Vision;Wnt signaling pathway SUBCELLULAR LOCATION: Nucleus " Q24266,"PROTEIN NAMES: Transcription factor btd (Protein buttonhead) ORGANISM: Drosophila melanogaster (Fruit fly) FUNCTION: Required for the development of the antennal, intercalary and mandibular segments of the head. KEYWORDS: Activator;DNA-binding;Metal-binding;Nucleus;Reference proteome;Repeat;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus " Q24317,"PROTEIN NAMES: DNA primase small subunit (DNA polymerase subunit A) (DNA polymerase subunit gamma) (DNA primase 50 kDa subunit) (dPRI50) PROTEIN FAMILY: Eukaryotic-type primase small subunit family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the eukaryotic-type primase small subunit family. FUNCTION: Catalytic subunit of the DNA primase complex and component of the DNA polymerase alpha complex (also known as the alpha DNA polymerase-primase complex) which play an essential role in the initiation of DNA synthesis. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit PolA1, an accessory subunit PolA2 and two primase subunits, the catalytic subunit Prim1 and the regulatory subunit Prim2) is recruited to DNA at the replicative forks (By similarity). The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands (By similarity). These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively (By similarity). In the primase complex, both subunits are necessary for the initial di-nucleotide formation, but the extension of the primer depends only on the catalytic subunit (By similarity). Can add both ribo- and deoxynucleotides during elongation of the primers. Binds single stranded DNA (By similarity). KEYWORDS: DNA replication;DNA-directed RNA polymerase;Metal-binding;Nucleotidyltransferase;Primosome;Reference proteome;Transcription;Transferase;Zinc MISCELLANEOUS: The bound zinc ion is not a cofactor. It is bound to a zinc knuckle motif that may be involved in sequence recognition and the binding of ssDNA (By similarity)." Q24371,"PROTEIN NAMES: Protein lethal(2)denticleless (Protein DTL83) PROTEIN FAMILY: WD repeat cdt2 family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the WD repeat cdt2 family. FUNCTION: Substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control. The DCX(DTL) complex, also named CRL4(CDT2) complex, mediates the polyubiquitination and subsequent degradation of E2f during S phase. E2f degradation is necessary to ensure proper development. Substrates require their interaction with PCNA for their polyubiquitination: substrates interact with PCNA via their PIP-box, leading to recruit the DCX(DTL) complex. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Alternative splicing;Cell cycle;Cytoplasm;Phosphoprotein;Reference proteome;Repeat;Stress response;Ubl conjugation pathway;WD repeat SUBCELLULAR LOCATION: Cytoplasm " Q24423,PROTEIN NAMES: Zinc finger protein Noc (Zinc finger protein NocA) PROTEIN FAMILY: Elbow/Noc family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the Elbow/Noc family. FUNCTION: May negatively regulate Notch-induced cell proliferation in the eye-head primordium. Required for development of the supraesophageal ganglion and ocelli. May act in leg and wing primordia to negatively regulate body-wall specifying genes and thereby promote appendage formation. Plays a role in tracheal development. KEYWORDS: Developmental protein;DNA-binding;Metal-binding;Notch signaling pathway;Phosphoprotein;Reference proteome;Zinc;Zinc-finger Q24488,"PROTEIN NAMES: Tyrosine-protein kinase transmembrane receptor Ror (dRor) PROTEIN FAMILY: Protein kinase superfamily, Tyr protein kinase family, ROR subfamily ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. ROR subfamily. FUNCTION: Tyrosine-protein kinase receptor that functions during early stages of neuronal development. KEYWORDS: ATP-binding;Developmental protein;Disulfide bond;Glycoprotein;Kinase;Kringle;Membrane;Nucleotide-binding;Phosphoprotein;Receptor;Reference proteome;Signal;Transferase;Transmembrane;Transmembrane helix;Tyrosine-protein kinase SUBCELLULAR LOCATION: Membrane ; Single-pass type I membrane protein " Q24492,"PROTEIN NAMES: Replication protein A 70 kDa DNA-binding subunit (RP-A p70) (Replication factor A protein 1) (RF-A protein 1) (Single-stranded DNA-binding protein) (DmRPA1) PROTEIN FAMILY: Replication factor A protein 1 family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the replication factor A protein 1 family. FUNCTION: As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. KEYWORDS: DNA replication;DNA-binding;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus. " Q24564,"PROTEIN NAMES: Moesin/ezrin/radixin homolog 2 (Ezrin-moesin-radixin 2) (Merlin) (dMerlin) ORGANISM: Drosophila melanogaster (Fruit fly) FUNCTION: Regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein Hippo (Hpo), in complex with its regulatory protein Salvador (Sav), phosphorylates and activates Warts (Wts) in complex with its regulatory protein Mats, which in turn phosphorylates and inactivates the Yorkie (Yki) oncoprotein. Mer acts synergistically along with Ex and Kibra to regulate the Hippo signaling pathway. KEYWORDS: 3D-structure;Cell junction;Cell membrane;Cell projection;Cytoplasm;Cytoskeleton;Endocytosis;Membrane;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Cell junction, adherens junction. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytoskeleton. Apical cell membrane. Cell projection, rhabdomere Note=Membrane-associated, adherens junctions and endocytic compartments. Cytoplasmic, punctate. " Q24567,"PROTEIN NAMES: Netrin-A ORGANISM: Drosophila melanogaster (Fruit fly) FUNCTION: Netrins control guidance of CNS commissural axons at the midline and peripheral motor axons to their target muscles. KEYWORDS: Developmental protein;Differentiation;Disulfide bond;Extracellular matrix;Glycoprotein;Laminin EGF-like domain;Neurogenesis;Reference proteome;Repeat;Secreted;Signal SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix. " Q24803,PROTEIN NAMES: Aldehyde-alcohol dehydrogenase 2 [Includes: Alcohol dehydrogenase (ADH) ; Acetaldehyde dehydrogenase (ACDH) ] PROTEIN FAMILY: Aldehyde dehydrogenase family; Iron-containing alcohol dehydrogenase family ORGANISM: Entamoeba histolytica (strain ATCC 30459 / HM-1:IMSS / ABRM) SIMILARITY: In the N-terminal section; belongs to the aldehyde dehydrogenase family.; SIMILARITY: In the C-terminal section; belongs to the iron-containing alcohol dehydrogenase family. FUNCTION: This enzyme has two NAD(+)-dependent activities: ADH and ACDH. May be a critical enzyme in the fermentative pathway. KEYWORDS: Direct protein sequencing;Iron;Multifunctional enzyme;NAD;Oxidoreductase;Reference proteome Q26261,"PROTEIN NAMES: Netrin receptor unc-5 (Uncoordinated protein 5) PROTEIN FAMILY: Unc-5 family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the unc-5 family. FUNCTION: Receptor for netrin (unc-6) required for axon guidance. Mediates axon repulsion of neuronal growth cones in the developing nervous system upon ligand binding. Axon migration is mediated by the secreted unc-6, which promotes attraction of neurons and axons through binding to the unc-40 receptor, while repulsion requires both unc-5 and unc-40 receptors. Involved in the ventral-dorsal and anterior-posterior migration of distal tip cells along the body, which may be mediated by Wnt receptor mom-5, ced-10/Rac, ced-12/ELMO and mig-2/RhoG. KEYWORDS: 3D-structure;Alternative splicing;Cell membrane;Cell projection;Developmental protein;Disulfide bond;Glycoprotein;Immunoglobulin domain;Membrane;Phosphoprotein;Receptor;Reference proteome;Repeat;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein Membrane raft Cell projection, neuron projection " Q26263,"PROTEIN NAMES: Protein deadpan ORGANISM: Drosophila melanogaster (Fruit fly) FUNCTION: Transcriptional repressor of genes that require a bHLH protein for their transcription. In the larval brain, required to maintain the self-renewal and identity of type II neuroblasts by regulating the expression of the transcriptional repressor erm together with other self-renewal transcriptional repressors such as klu and E(spl)mgamma-HLH. As part of its role in neuroblasts development, has been shown to be a direct target of the Notch signaling pathway, however might work also independently of N/Notch. In the developing larval and pupal brain, required for mushroom body differentiation. Involved in sex determination and SXL transcription repression when in complex with the corepressor protein Groucho. KEYWORDS: Developmental protein;Differentiation;DNA-binding;Neurogenesis;Nucleus;Phosphoprotein;Reference proteome;Repressor;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " Q26366,"PROTEIN NAMES: Protein vestigial ORGANISM: Drosophila melanogaster (Fruit fly) FUNCTION: Involved in determining which thoracic imaginal disk cells will form wings and halteres, perhaps by interacting with other nuclear regulatory proteins. When in combination with scalloped (sd), it acts as a transcriptional activation complex that regulates gene expression in the wing. Binding to sd switches the DNA target selectivity of sd. Required and sufficient for cell proliferation at the dorsal/ventral (D/V) boundary of the wing imaginal disk. Also required for cell proliferation in the wing imaginal disk, mediated via activation of E2f. By interacting with Dhfr, may control genes involved in DNA replication. KEYWORDS: 3D-structure;Activator;Cell cycle;Developmental protein;DNA replication;DNA-binding;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus. MISCELLANEOUS: Loss of vestigial function selectively eliminates wing and haltere formation." Q26609,PROTEIN NAMES: Hexokinase PROTEIN FAMILY: Hexokinase family ORGANISM: Schistosoma mansoni (Blood fluke) SIMILARITY: Belongs to the hexokinase family. FUNCTION: Catalyzes the phosphorylation of various hexoses to hexose 6-phosphate. PATHWAY: Carbohydrate metabolism; hexose metabolism.; PATHWAY: Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 1/4. KEYWORDS: 3D-structure;ATP-binding;Glycolysis;Kinase;Nucleotide-binding;Reference proteome;Transferase Q26710,"PROTEIN NAMES: Alternative oxidase, mitochondrial PROTEIN FAMILY: Alternative oxidase family ORGANISM: Trypanosoma brucei brucei SIMILARITY: Belongs to the alternative oxidase family. FUNCTION: Catalyzes cyanide-resistant oxygen consumption. May increase respiration when the cytochrome respiratory pathway is restricted, or in response to low temperatures. KEYWORDS: 3D-structure;Disulfide bond;Electron transport;Iron;Membrane;Metal-binding;Mitochondrion;Mitochondrion inner membrane;Oxidoreductase;Respiratory chain;Transit peptide;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Mitochondrion inner membrane ; Multi-pass membrane protein Note=Mitochondrial, possibly in the inner surface of the inner mitochondrial membrane. " Q27081,"PROTEIN NAMES: Clotting factor B (Coagulation factor B) [Cleaved into: Clotting factor B light chain; Clotting factor B heavy chain] PROTEIN FAMILY: Peptidase S1 family, CLIP subfamily ORGANISM: Tachypleus tridentatus (Japanese horseshoe crab) SIMILARITY: Belongs to the peptidase S1 family. CLIP subfamily. FUNCTION: This enzyme is closely associated with an endotoxin-sensitive hemolymph coagulation system which may play important roles in both hemostasis and host defense mechanisms. Its active form catalyzes the activation of proclotting enzyme. Does not activate the mammalian coagulation factors factor IX, factor X, prothrombin, plasminogen, protein C or prekallikrein. Does not hydrolyze fibrinogen. Does not catalyze the activation of factor C or coagulogen. KEYWORDS: Direct protein sequencing;Disulfide bond;Glycoprotein;Hemolymph clotting;Hydrolase;Protease;Secreted;Serine protease;Signal;Zymogen SUBCELLULAR LOCATION: Secreted Note=Secreted in hemolymph. " Q27083,"PROTEIN NAMES: Clotting factor G beta subunit [Cleaved into: Clotting factor G beta subunit light chain; Clotting factor G beta subunit heavy chain] PROTEIN FAMILY: Peptidase S1 family ORGANISM: Tachypleus tridentatus (Japanese horseshoe crab) SIMILARITY: Belongs to the peptidase S1 family. FUNCTION: Component of the heterodimer clotting factor G which may play a role in defense mechanisms against fungi (Probable). Initiates a (1->3)-beta-glucan-sensing clotting pathway whereby the alpha subunit binds to glucans containing (1->3)-beta linkages, which are components of the fungal cell wall, and the beta subunit catalyzes the activation of proclotting enzyme. KEYWORDS: Direct protein sequencing;Disulfide bond;Glycoprotein;Hemolymph clotting;Hydrolase;Protease;Serine protease;Signal;Zymogen " Q27218,"PROTEIN NAMES: Acetylcholine receptor subunit beta-type lev-1 (Levamisole-resistant protein 1) PROTEIN FAMILY: Ligand-gated ion channel family, Acetylcholine receptor subfamily ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. FUNCTION: Non-alpha subunit of nicotinic acetylcholine receptor (nAChR). Involved in nAChR sensitivity to nicotine. KEYWORDS: Cell membrane;Disulfide bond;Glycoprotein;Ion channel;Ion transport;Ligand-gated ion channel;Membrane;Postsynaptic cell membrane;Receptor;Reference proteome;Signal;Synapse;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Postsynaptic cell membrane ; Multi-pass membrane protein Cell membrane ; Multi-pass membrane protein Note=Co-localizes with unc-29 and unc-38 at nerve cord synapses. " Q27294,PROTEIN NAMES: RNA-binding protein cabeza (P19) (Sarcoma-associated RNA-binding fly homolog) PROTEIN FAMILY: RRM TET family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the RRM TET family. FUNCTION: May participate in a function common to the expression of most genes transcribed by RNA polymerase II. KEYWORDS: Metal-binding;Nucleus;Reference proteome;RNA-binding;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: 'Cabeza' means 'head' in Spanish. Q27355,"PROTEIN NAMES: Transcription factor lin-26 (Abnormal cell lineage protein 26) ORGANISM: Caenorhabditis elegans FUNCTION: Probable transcription factor. Required to specify the fates of hypodermal and neuron-associated support cells. Functions during vulval development, playing a role in vulval precursor cell fate specification. Positively modulates expression of homeobox protein lin-39, perhaps by binding to regulatory regions of the lin-39 gene, acting in the vulval lineage. KEYWORDS: Alternative splicing;DNA-binding;Metal-binding;Nucleus;Reference proteome;Repeat;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus " Q27367,"PROTEIN NAMES: Protein croquemort (d-CD36) PROTEIN FAMILY: CD36 family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the CD36 family. FUNCTION: Macrophage receptor for apoptotic cells. Up-regulates Bfc, which in turn leads to self-up-regulation by Bfc in a positive feedback mechanism in response to detection of apoptotic cells. KEYWORDS: Apoptosis;Cell membrane;Disulfide bond;Glycoprotein;Lipoprotein;Membrane;Palmitate;Receptor;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein MISCELLANEOUS: 'Croquemort' means literally 'the one who bites dead persons' in French and is colloquial for undertaker." Q27403,PROTEIN NAMES: Transcription factor glial cells missing (Protein glide) ORGANISM: Drosophila melanogaster (Fruit fly) FUNCTION: Transcription factor that induces gliogenesis. It determines the choice between glial and neuronal fates. Also has a role in the differentiation of the plasmatocyte/macrophage lineage of hemocytes. KEYWORDS: Developmental protein;DNA-binding;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus Q27433,"PROTEIN NAMES: Mechanosensory protein 2 PROTEIN FAMILY: Band 7/mec-2 family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the band 7/mec-2 family. FUNCTION: Subunit of an amiloride-sensitive cation channel (degenerin channel complex) permeable for sodium, potassium, lithium and N-methylglucamine, and required for mechanosensory transduction (touch sensitivity). Positively regulates the activity of the putative mechanosensory transduction channel. May link the mechanosensory channel and the microtubule cytoskeleton of the touch receptor neurons. Required for the function of a set of six touch receptor neurons. KEYWORDS: Ion channel;Ion transport;Membrane;Potassium;Potassium transport;Reference proteome;Sodium;Sodium channel;Sodium transport;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Membrane ; Single-pass membrane protein " Q27651,"PROTEIN NAMES: ATP-dependent 6-phosphofructokinase (ATP-PFK) (Phosphofructokinase) (Phosphohexokinase) PROTEIN FAMILY: Phosphofructokinase type A (PFKA) family, PPi-dependent PFK group II subfamily, Atypical ATP-dependent clade 'X' sub-subfamily ORGANISM: Entamoeba histolytica (strain ATCC 30459 / HM-1:IMSS / ABRM) SIMILARITY: Belongs to the phosphofructokinase type A (PFKA) family. PPi-dependent PFK group II subfamily. Atypical ATP-dependent clade 'X' sub-subfamily. FUNCTION: Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis. PATHWAY: Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4. KEYWORDS: Allosteric enzyme;ATP-binding;Cytoplasm;Glycolysis;Kinase;Magnesium;Metal-binding;Nucleotide-binding;Reference proteome;Transferase SUBCELLULAR LOCATION: Cytoplasm " Q27705,"PROTEIN NAMES: Pyrophosphate--fructose 6-phosphate 1-phosphotransferase (6-phosphofructokinase, pyrophosphate dependent) (PPi-dependent phosphofructokinase) (PPi-PFK) (Pyrophosphate-dependent 6-phosphofructose-1-kinase) PROTEIN FAMILY: Phosphofructokinase type A (PFKA) family, PPi-dependent PFK group II subfamily, Clade 'Short' sub-subfamily ORGANISM: Naegleria fowleri (Brain eating amoeba) SIMILARITY: Belongs to the phosphofructokinase type A (PFKA) family. PPi-dependent PFK group II subfamily. Clade 'Short' sub-subfamily. FUNCTION: Catalyzes the phosphorylation of D-fructose 6-phosphate, the first committing step of glycolysis. Uses inorganic phosphate (PPi) as phosphoryl donor instead of ATP like common ATP-dependent phosphofructokinases (ATP-PFKs), which renders the reaction reversible, and can thus function both in glycolysis and gluconeogenesis. Consistently, PPi-PFK can replace the enzymes of both the forward (ATP-PFK) and reverse (fructose-bisphosphatase (FBPase)) reactions. PATHWAY: Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4. KEYWORDS: Cytoplasm;Glycolysis;Kinase;Magnesium;Metal-binding;Transferase SUBCELLULAR LOCATION: Cytoplasm " Q27886,"PROTEIN NAMES: Abnormal cell lineage protein 44 (Wnt protein) PROTEIN FAMILY: Wnt family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the Wnt family. FUNCTION: Ligand for members of the frizzled family of seven transmembrane receptors (By similarity). Affects male tail development, vulval precursor cell specification and egg laying. Involved in morphogenesis by influencing polarity of asymmetric cell divisions of the B, U, and F cells in the male, and the T cell in males and hermaphrodites. Controls spindle orientation in B-gamma cell division during male copulatory spicule development. Involved in specification of the P7.p lineage during vulval development. Has a role in providing polarity and default lin-17 localization in axon development and positioning of neuromuscular synapses in DA9 regions by negatively regulating synaptogenesis. Plays a role in motorneuron development by promoting the extension of the anterior neurite of ventral D-type GABAergic motorneurons along the anterior-posterior axis of the ventral nerve cord. Positively regulates cilium position and dendrite morphogenesis in postembryonic PQR gas-sensing neurons. This is likely through regulating the localization of grdn-1 to the distal dendrites of PQR sensory neurons. KEYWORDS: Developmental protein;Disulfide bond;Extracellular matrix;Glycoprotein;Lipoprotein;Reference proteome;Secreted;Signal;Wnt signaling pathway SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix " Q27934,"PROTEIN NAMES: Protein phyllopod ORGANISM: Drosophila melanogaster (Fruit fly) FUNCTION: Essential adapter component of E3 ubiquitin ligase complexes; involved in R7 photoreceptor cell differentiation, embryonic nervous system, external sensory organ development and specification of particular muscles. E3 ubiquitin ligase complexes mediate ubiquitination and subsequent proteasomal degradation of target proteins. Required for specification of R7 photoreceptor cell fate in the eye by participating in the ubiquitination and subsequent proteasomal degradation of Tramtrack (ttk), a general inhibitor of photoreceptor differentiation. Acts downstream of Notch signaling to specify the fate of the SOP (sensory organ precursor) cells and their progeny, probably via the sina-mediated proteasomal degradation of ttk. Its restricted pattern of expression, upon Notch and Ras signaling pathways, suggests that it acts as a key determinant in E3 complexes to trigger protein proteolysis in appropriate cells. KEYWORDS: 3D-structure;Cell cycle;Coiled coil;Notch signaling pathway;Nucleus;Reference proteome;Sensory transduction;Ubl conjugation pathway;Vision SUBCELLULAR LOCATION: Nucleus " Q28557,PROTEIN NAMES: Corticotropin-releasing factor-binding protein (CRF-BP) (CRF-binding protein) (Corticotropin-releasing hormone-binding protein) (CRH-BP) PROTEIN FAMILY: CRF-binding protein family ORGANISM: Ovis aries (Sheep) SIMILARITY: Belongs to the CRF-binding protein family. FUNCTION: Binds CRF and inactivates it. May prevent inappropriate pituitary-adrenal stimulation in pregnancy. KEYWORDS: Disulfide bond;Glycoprotein;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted Q28615,"PROTEIN NAMES: Solute carrier family 13 member 2 (Na(+)/dicarboxylate cotransporter 1) (NaDC-1) (Renal sodium/dicarboxylate cotransporter) PROTEIN FAMILY: SLC13A/DASS transporter family, NADC subfamily ORGANISM: Oryctolagus cuniculus (Rabbit) SIMILARITY: Belongs to the SLC13A/DASS transporter (TC 2.A.47) family. NADC subfamily. FUNCTION: Low-affinity sodium-dicarboxylate cotransporter, that mediates the entry of citric acid cycle intermediates, such as succinate, citrate, fumarate and alpha-ketoglutarate (2-oxoglutarate) into the small intestine and renal proximal tubule (By similarity). Transports the dicarboxylate into the cell with a probable stoichiometry of 3 Na(+) for 1 divalent dicarboxylate, rendering the process electrogenic. Citrate is transported in protonated form as a divalent anion, rather than the trivalent form which is normally found in blood. Has a critical role in renal dicarboxylate transport (By similarity). KEYWORDS: Cell membrane;Ion transport;Membrane;Reference proteome;Sodium;Sodium transport;Symport;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Apical cell membrane ; Multi-pass membrane protein " Q28719,"PROTEIN NAMES: Prostaglandin reductase 1 (PRG-1) (15-oxoprostaglandin 13-reductase) (ADRAB-F) (Dithiolethione-inducible gene 1 protein) (D3T-inducible gene 1 protein) (DIG-1) (Leukotriene B4 12-hydroxydehydrogenase) (NAD(P)H-dependent alkenal/one oxidoreductase) PROTEIN FAMILY: NADP-dependent oxidoreductase L4BD family ORGANISM: Oryctolagus cuniculus (Rabbit) SIMILARITY: Belongs to the NADP-dependent oxidoreductase L4BD family. FUNCTION: NAD(P)H-dependent oxidoreductase involved in metabolic inactivation of pro- and anti-inflammatory eicosanoids: prostaglandins (PG), leukotrienes (LT) and lipoxins (LX). Catalyzes with high efficiency the reduction of the 13,14 double bond of 15-oxoPGs, including 15-oxo-PGE1, 15-oxo-PGE2, 15-oxo-PGF1-alpha and 15-oxo-PGF2-alpha (By similarity). Catalyzes with lower efficiency the oxidation of the hydroxyl group at C12 of LTB4 and its derivatives, converting them into biologically less active 12-oxo-LTB4 metabolites (By similarity). Reduces 15-oxo-LXA4 to 13,14 dihydro-15-oxo-LXA4, enhancing neutrophil recruitment at the inflammatory site (By similarity). Plays a role in metabolic detoxification of alkenals and ketones. Reduces alpha,beta-unsaturated alkenals and ketones, particularly those with medium-chain length, showing highest affinity toward (2E)-decenal and (3E)-3-nonen-2-one (By similarity). May inactivate 4-hydroxy-2-nonenal, a cytotoxic lipid constituent of oxidized low-density lipoprotein particles (By similarity). KEYWORDS: Acetylation;Cytoplasm;Fatty acid metabolism;Hydroxylation;Lipid metabolism;NADP;Oxidoreductase;Phosphoprotein;Prostaglandin metabolism;Reference proteome SUBCELLULAR LOCATION: Cytoplasm " Q28858,"PROTEIN NAMES: Versican core protein (Chondroitin sulfate proteoglycan core protein 2) (Chondroitin sulfate proteoglycan 2) (Large fibroblast proteoglycan) PROTEIN FAMILY: Aggrecan/versican proteoglycan family ORGANISM: Macaca nemestrina (Pig-tailed macaque) SIMILARITY: Belongs to the aggrecan/versican proteoglycan family. FUNCTION: May play a role in intercellular signaling and in connecting cells with the extracellular matrix. May take part in the regulation of cell motility, growth and differentiation. Binds hyaluronan. KEYWORDS: Calcium;Cell projection;Disulfide bond;EGF-like domain;Extracellular matrix;Glycoprotein;Lectin;Phosphoprotein;Proteoglycan;Reference proteome;Repeat;Secreted SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix Cell projection, cilium, photoreceptor outer segment Secreted, extracellular space, extracellular matrix, interphotoreceptor matrix " Q2A865,"PROTEIN NAMES: Sodium-dependent neutral amino acid transporter B(0)AT1 (Solute carrier family 6 member 19) (System B(0) neutral amino acid transporter AT1) PROTEIN FAMILY: Sodium:neurotransmitter symporter (SNF) family, SLC6A19 subfamily ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A19 subfamily. FUNCTION: Transporter that mediates resorption of neutral amino acids across the apical membrane of renal and intestinal epithelial cells. This uptake is sodium-dependent and chloride-independent. Requires CLTRN in kidney or ACE2 in intestine for cell surface expression and amino acid transporter activity. KEYWORDS: Amino-acid transport;Glycoprotein;Membrane;Phosphoprotein;Reference proteome;Symport;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Membrane ; Multi-pass membrane protein " Q2CEE3,PROTEIN NAMES: Protein O-GlcNAcase (OGA) (Beta-N-acetylglucosaminidase) (Beta-N-acetylhexosaminidase) (Beta-hexosaminidase) (N-acetyl-beta-D-glucosaminidase) (N-acetyl-beta-glucosaminidase) PROTEIN FAMILY: Glycosyl hydrolase 84 family ORGANISM: Oceanicola granulosus (strain ATCC BAA-861 / DSM 15982 / KCTC 12143 / HTCC2516) SIMILARITY: Belongs to the glycosyl hydrolase 84 family. FUNCTION: Cleaves GlcNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrate (in vitro). KEYWORDS: 3D-structure;Glycosidase;Hydrolase;Reference proteome Q2HJF9,"PROTEIN NAMES: S-adenosylmethionine-dependent nucleotide dehydratase RSAD2 (SAND) (Radical S-adenosyl methionine domain-containing protein 2) (Virus inhibitory protein, endoplasmic reticulum-associated, interferon-inducible) (Viperin) PROTEIN FAMILY: Radical SAM superfamily, RSAD2 family ORGANISM: Bos taurus (Bovine) SIMILARITY: Belongs to the radical SAM superfamily. RSAD2 family. FUNCTION: Interferon-inducible antiviral protein which plays a major role in the cell antiviral state induced by type I and type II interferon. Catalyszes the conversion of cytidine triphosphate (CTP) to 3'-deoxy-3',4'-didehydro-CTP (ddhCTP) via a SAM-dependent radical mechanism. In turn, ddhCTP acts as a chain terminator for the RNA-dependent RNA polymerases from multiple viruses and directly inhibits viral replication. Therefore, inhibits a wide range of DNA and RNA viruses. Promotes also TLR7 and TLR9-dependent production of IFN-beta production in plasmacytoid dendritic cells (pDCs) by facilitating 'Lys-63'-linked ubiquitination of IRAK1 by TRAF6. Plays a role in CD4+ T-cells activation and differentiation. Facilitates T-cell receptor (TCR)-mediated GATA3 activation and optimal T-helper 2 (Th2) cytokine production by modulating NFKB1 and JUNB activities. Can inhibit secretion of soluble proteins. KEYWORDS: 4Fe-4S;Acetylation;Antiviral defense;Endoplasmic reticulum;Golgi apparatus;Immunity;Innate immunity;Iron;Iron-sulfur;Isopeptide bond;Lipid droplet;Lyase;Membrane;Metal-binding;Mitochondrion;Mitochondrion inner membrane;Mitochondrion outer membrane;Reference proteome;S-adenosyl-L-methionine;Ubl conjugation SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Peripheral membrane protein ; Cytoplasmic side Golgi apparatus Endoplasmic reticulum Lipid droplet Mitochondrion Mitochondrion inner membrane Mitochondrion outer membrane " Q2I0M5,"PROTEIN NAMES: R-spondin-4 (Roof plate-specific spondin-4) (hRspo4) PROTEIN FAMILY: R-spondin family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the R-spondin family. FUNCTION: Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. KEYWORDS: Alternative splicing;Disease variant;Disulfide bond;Glycoprotein;Heparin-binding;Phosphoprotein;Reference proteome;Secreted;Sensory transduction;Signal;Wnt signaling pathway SUBCELLULAR LOCATION: Secreted " Q2JJF6,PROTEIN NAMES: Vitamin K epoxide reductase homolog (VKOR) PROTEIN FAMILY: VKOR family ORGANISM: Synechococcus sp. (strain JA-2-3B'a(2-13)) (Cyanobacteria bacterium Yellowstone B-Prime) SIMILARITY: Belongs to the VKOR family. FUNCTION: Thiol-disulfide oxidoreductase that catalyzes vitamin K-dependent disulfide bond formation in periplasmic target proteins. KEYWORDS: 3D-structure;Disulfide bond;Membrane;Oxidoreductase;Quinone;Redox-active center;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Multi-pass membrane protein Q2KJ53,"PROTEIN NAMES: Golgi-resident adenosine 3',5'-bisphosphate 3'-phosphatase (Golgi-resident PAP phosphatase) (gPAPP) (3'(2'), 5'-bisphosphate nucleotidase 2) (Inositol monophosphatase domain-containing protein 1) (Myo-inositol monophosphatase A3) (Phosphoadenosine phosphate 3'-nucleotidase) PROTEIN FAMILY: Inositol monophosphatase superfamily ORGANISM: Bos taurus (Bovine) SIMILARITY: Belongs to the inositol monophosphatase superfamily. FUNCTION: Exhibits 3'-nucleotidase activity toward adenosine 3',5'-bisphosphate (PAP), namely hydrolyzes adenosine 3',5'-bisphosphate into adenosine 5'-monophosphate (AMP) and a phosphate. May play a role in the formation of skeletal elements derived through endochondral ossification, possibly by clearing adenosine 3',5'-bisphosphate produced by Golgi sulfotransferases during glycosaminoglycan sulfation. Has no activity toward 3'-phosphoadenosine 5'-phosphosulfate (PAPS) or inositol phosphate (IP) substrates including I(1)P, I(1,4)P2, I(1,3,4)P3, I(1,4,5)P3 and I(1,3,4,5)P4. PATHWAY: Sulfur metabolism. KEYWORDS: Acetylation;Glycoprotein;Golgi apparatus;Hydrolase;Magnesium;Membrane;Metal-binding;Reference proteome;Signal-anchor;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus Golgi apparatus, trans-Golgi network membrane ; Single-pass type II membrane protein Note=The catalytic core is predicted to reside within the Golgi lumen. " Q2L4W6,PROTEIN NAMES: Sporulation-specific protein 5 (Meiotic RNA-binding protein 1) (Meiotically up-regulated gene 12 protein) ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) FUNCTION: RNA-binding protein which plays a role in sporulation. Regulates the progression of meiosis I and may function in the vicinity of the Mei2 dot. KEYWORDS: Cytoplasm;Meiosis;Reference proteome;Repeat;RNA-binding;Sporulation SUBCELLULAR LOCATION: Cytoplasm Q2M405,"PROTEIN NAMES: Crinkler effector protein 8 PROTEIN FAMILY: Crinkler effector family; Protein kinase superfamily ORGANISM: Phytophthora infestans (Potato late blight agent) (Botrytis infestans) SIMILARITY: In the N-terminal section; belongs to the Crinkler effector family.; SIMILARITY: In the C-terminal section; belongs to the protein kinase superfamily. FUNCTION: Secreted effector that induces cell death when expressed in host plants. Acts as a kinase and is able to autophosphorylate, however its cell death inducing ability is not a direct result of its kinase activity, but rather a consequence of the phosphorylated state of the five identified serine residues in the CRN8 protein. KEYWORDS: Glycoprotein;Host nucleus;Kinase;Phosphoprotein;Secreted;Serine/threonine-protein kinase;Signal;Transferase;Virulence SUBCELLULAR LOCATION: Secreted Host nucleus " Q2PS07,PROTEIN NAMES: 2S seed storage albumin protein (16 kDa buckwheat protein) (BWp16) (2S albumin) (2S seed storage protein) (Buckwheat 16 kDa major allergen) (allergen Fag e 2.0101) PROTEIN FAMILY: 2S seed storage albumins family ORGANISM: Fagopyrum esculentum (Common buckwheat) (Polygonum fagopyrum) SIMILARITY: Belongs to the 2S seed storage albumins family. FUNCTION: Seed storage protein. KEYWORDS: Allergen;Direct protein sequencing;Disulfide bond;IgE-binding protein;Seed storage protein;Signal;Storage protein Q2QUC5,PROTEIN NAMES: Tryptamine 5-hydroxylase (Cytochrome P450 71P1) (Protein SEKIGUCHI LESION) PROTEIN FAMILY: Cytochrome P450 family ORGANISM: Oryza sativa subsp. japonica (Rice) SIMILARITY: Belongs to the cytochrome P450 family. FUNCTION: Involved in serotonin biosynthesis. Catalyzes the conversion of tryptamine to serotonin. Accumulation of serotonin may play a role in innate immunity. KEYWORDS: Endoplasmic reticulum;Heme;Iron;Membrane;Metal-binding;Monooxygenase;Oxidoreductase;Plant defense;Reference proteome;Serotonin biosynthesis;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Single-pass membrane protein Q2TAK8,PROTEIN NAMES: PWWP domain-containing DNA repair factor 3A (PWWP3A) (Mutated melanoma-associated antigen 1) (MUM-1) (PWWP domain-containing protein MUM1) (Protein expandere) PROTEIN FAMILY: PWWP3A family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the PWWP3A family. FUNCTION: Involved in the DNA damage response pathway by contributing to the maintenance of chromatin architecture. Recruited to the vicinity of DNA breaks by TP53BP1 and plays an accessory role to facilitate damage-induced chromatin changes and promoting chromatin relaxation. Required for efficient DNA repair and cell survival following DNA damage. KEYWORDS: 3D-structure;Alternative splicing;DNA damage;DNA repair;Nucleus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Nucleus Note=Recruited to DNA damage sites via its interaction with the BRCT domain of TP53BP1. MISCELLANEOUS: Acts as an antigenic peptide recognized by cytolytic T-lymphocytes in a melanoma. Q2TCH4,"PROTEIN NAMES: Transforming growth factor beta-1-induced transcript 1 protein (Androgen receptor activator of 55 kDa) (Hydrogen peroxide-inducible clone 5 protein) (Hic-5) PROTEIN FAMILY: Paxillin family ORGANISM: Xenopus laevis (African clawed frog) SIMILARITY: Belongs to the paxillin family. FUNCTION: Functions as a molecular adapter coordinating multiple protein-protein interactions at the focal adhesion complex and in the nucleus. May regulate both Wnt and steroid signaling pathways and play a role in the processes of cell growth, proliferation, migration, differentiation and senescence. May have a zinc-dependent DNA-binding activity. KEYWORDS: Activator;Cell junction;Cytoplasm;Cytoskeleton;Differentiation;LIM domain;Metal-binding;Nucleus;Reference proteome;Repeat;Wnt signaling pathway;Zinc SUBCELLULAR LOCATION: Cell junction, focal adhesion Nucleus matrix Cytoplasm, cytoskeleton Note=Associated with the actin cytoskeleton; colocalizes with stress fibers. " Q2VEQ7,"PROTEIN NAMES: D-2-hydroxyacid dehydrogenase (D2-HDH) (D-specific 2-hydroxyacid dehydrogenase) PROTEIN FAMILY: D-isomer specific 2-hydroxyacid dehydrogenase family ORGANISM: Haloferax mediterranei (strain ATCC 33500 / DSM 1411 / JCM 8866 / NBRC 14739 / NCIMB 2177 / R-4) (Halobacterium mediterranei) SIMILARITY: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. FUNCTION: Catalyzes the stereospecific NAD(P)H-dependent reduction of 2-ketocarboxylic acids into the corresponding D-2-hydroxycarboxylic acids. Can use both NADPH or NADH as reductant, displaying a marked preference for NADPH over NADH. Shows a broad substrate specificity, although it displays a marked preference for the 2-ketocarboxylic acids having an unbranched chain of 4-5 carbon atoms. KEYWORDS: 3D-structure;NAD;NADP;Oxidoreductase " Q2W8Q2,"PROTEIN NAMES: Probable membrane transporter protein MamO PROTEIN FAMILY: Peptidase S1C family; 4-toluene sulfonate uptake permease (TSUP) family ORGANISM: Paramagnetospirillum magneticum (strain ATCC 700264 / AMB-1) (Magnetospirillum magneticum) SIMILARITY: In the N-terminal section; belongs to the peptidase S1C family.; SIMILARITY: In the C-terminal section; belongs to the 4-toluene sulfonate uptake permease (TSUP) (TC 2.A.102) family. FUNCTION: Plays 2 roles; promotes magnetite nucleation/formation and activates the MamE protease (Probable). Despite its near conservation of a protease-like sequence, this is probably not a protease (Probable). Required in conjunction with MamP for proteolysis of at least MamE, itself and MamP. May transport a solute that controls MamE's protease activity. May place individual iron atoms into the magnetite lattice (Probable). KEYWORDS: 3D-structure;Biomineralization;Direct protein sequencing;Magnetosome;Membrane;Metal-binding;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Magnetosome membrane ; Multi-pass membrane protein MISCELLANEOUS: This bacteria makes up to 20 cubo-octahedral magnetosomes of about 45 nm in diameter which contain membrane-bound crystals of magnetite (Fe(3)O(4)).; MISCELLANEOUS: There is a paralogous gene with partially overlapping function in the genome (amb1004, called limO, AC Q2W8L7). LimO does not encode the C-terminal TSUP domain and does not complement a mamO deletion.; MISCELLANEOUS: Expression of just the minimal mamAB gene cluster (amb0961 to amb0978), including this gene, is sufficient to form a minimal magnetosome chain with small magnetite particles." Q2WG80,"PROTEIN NAMES: Protein ripply1 PROTEIN FAMILY: Ripply family ORGANISM: Danio rerio (Zebrafish) (Brachydanio rerio) SIMILARITY: Belongs to the ripply family. FUNCTION: Plays a role in somitogenesis. Essential for transcriptional repression of the segmental patterning genes, thus terminating the segmentation program in the presomitic mesoderm, and also required for the maintenance of rostrocaudal polarity in somites. KEYWORDS: Developmental protein;Nucleus;Reference proteome;Repressor;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: Named after the expression pattern, which decreases in a posterior to anterior direction in somites like a ripple." Q30W70,"PROTEIN NAMES: Choline trimethylamine-lyase (Choline TMA-lyase) (Choline utilization protein C) (Glycyl radical enzyme CutC) (GRE CutC) PROTEIN FAMILY: Glycyl radical enzyme (GRE) family, CutC subfamily ORGANISM: Oleidesulfovibrio alaskensis (strain ATCC BAA-1058 / DSM 17464 / G20) (Desulfovibrio alaskensis) SIMILARITY: Belongs to the glycyl radical enzyme (GRE) family. CutC subfamily. FUNCTION: Glycine radical enzyme that catalyzes the cleavage of a C-N bond in choline, producing trimethylamine (TMA) and acetaldehyde. Is involved in the anaerobic choline utilization pathway that allows D.alaskensis to grow on choline as a source of carbon and energy. Is strictly specific for choline as substrate. PATHWAY: Amine and polyamine metabolism; choline degradation. KEYWORDS: 3D-structure;Lyase;Organic radical;Reference proteome " Q31125,"PROTEIN NAMES: Zinc transporter SLC39A7 (Histidine-rich membrane protein Ke4) (Solute carrier family 39 member 7) (Zrt-, Irt-like protein 7) (ZIP7) PROTEIN FAMILY: ZIP transporter family, KE4/Catsup subfamily ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the ZIP transporter (TC 2.A.5) family. KE4/Catsup subfamily. FUNCTION: Transports Zn(2+) from the endoplasmic reticulum (ER)/Golgi apparatus to the cytosol, playing an essential role in the regulation of cytosolic zinc levels. Acts as a gatekeeper of zinc release from intracellular stores, requiring post-translational activation by phosphorylation, resulting in activation of multiple downstream pathways leading to cell growth and proliferation (By similarity). Has an essential role in B cell development and is required for proper B cell receptor signaling. Plays an important role in maintaining intestinal epithelial homeostasis and skin dermis development by regulating ER function. Controls cell signaling pathways involved in glucose metabolism in skeletal muscle. Has a protective role against ER stress in different biological contexts. Mediates Zn(2+)-induced ferroptosis (By similarity). KEYWORDS: Endoplasmic reticulum;Golgi apparatus;Ion transport;Membrane;Methylation;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix;Transport;Zinc;Zinc transport SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein Golgi apparatus, cis-Golgi network membrane ; Multi-pass membrane protein " Q32M88,PROTEIN NAMES: Protein-glucosylgalactosylhydroxylysine glucosidase (Acid trehalase-like protein 1) PROTEIN FAMILY: Glycosyl hydrolase 65 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the glycosyl hydrolase 65 family. FUNCTION: Catalyzes the hydrolysis of glucose from the disaccharide unit linked to hydroxylysine residues of collagen and collagen-like proteins. KEYWORDS: Alternative splicing;Glycosidase;Hydrolase;Reference proteome Q32MK0,"PROTEIN NAMES: Myosin light chain kinase 3 (Cardiac-MyBP-C-associated Ca/CaM kinase) (Cardiac-MLCK) PROTEIN FAMILY: Protein kinase superfamily, CAMK Ser/Thr protein kinase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. FUNCTION: Kinase that phosphorylates MYL2 in vitro. Promotes sarcomere formation in cardiomyocytes and increases cardiomyocyte contractility (By similarity). KEYWORDS: Alternative splicing;ATP-binding;Cytoplasm;Kinase;Magnesium;Nucleotide-binding;Phosphoprotein;Reference proteome;Serine/threonine-protein kinase;Transferase SUBCELLULAR LOCATION: Cytoplasm " Q6ZR37,PROTEIN NAMES: Pleckstrin homology domain-containing family G member 7 (PH domain-containing family G member 7) ORGANISM: Homo sapiens (Human) KEYWORDS: Alternative splicing;Glycoprotein;Reference proteome Q38796,PROTEIN NAMES: Homeobox protein LUMINIDEPENDENS ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Seems to play a role in the regulation of flowering time in the autonomous flowering pathway by repressing FLOWERING LOCUS C expression. KEYWORDS: Developmental protein;Differentiation;DNA-binding;Flowering;Homeobox;Nucleus;Reference proteome;Repeat SUBCELLULAR LOCATION: Nucleus. Q38897,"PROTEIN NAMES: Homeobox protein BEL1 homolog PROTEIN FAMILY: TALE/BELL homeobox family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the TALE/BELL homeobox family. FUNCTION: Plays a major role in ovule patterning and in determination of integument identity via its interaction with MADS-box factors. Formation of complex with AG-SEP dimers negatively regulates the carpel identity process and favors the maintenance of ovule identity. BEL1-STM complex maintains the indeterminacy of the inflorescence meristem. Required, with SPL, for cytokinin-induced PIN1 expression in ovules. KEYWORDS: DNA-binding;Homeobox;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " Q38906,"PROTEIN NAMES: Translocase of chloroplast 34, chloroplastic (AtToc34) (34 kDa chloroplast outer envelope protein) (GTP-binding protein OEP34) (Plastid protein import 3) PROTEIN FAMILY: TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily, AIG1/Toc34/Toc159-like paraseptin GTPase family, TOC34 subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. AIG1/Toc34/Toc159-like paraseptin GTPase family. TOC34 subfamily. FUNCTION: GTPase involved in protein precursor import into chloroplasts. Seems to recognize chloroplast-destined precursor proteins and regulate their presentation to the translocation channel through GTP hydrolysis. Probably specialized in the import of nuclear encoded non-photosynthetic preproteins from the cytoplasm to the chloroplast. KEYWORDS: Acetylation;Chloroplast;GTP-binding;Hydrolase;Magnesium;Membrane;Metal-binding;Nucleotide-binding;Plastid;Plastid outer membrane;Protein transport;Receptor;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Plastid, chloroplast outer membrane ; Single-pass membrane protein Note=May contain beta barrel transmembrane regions. " Q38920,PROTEIN NAMES: Protein farnesyltransferase subunit beta (FTase-beta) (CAAX farnesyltransferase subunit beta) (Enhanced response to abscisic acid 1) (Ras proteins prenyltransferase subunit beta) PROTEIN FAMILY: Protein prenyltransferase subunit beta family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the protein prenyltransferase subunit beta family. FUNCTION: Catalyzes the transfer of a farnesyl moiety from farnesyl diphosphate to a cysteine at the fourth position from the C-terminus of several proteins having the C-terminal sequence Cys-aliphatic-aliphatic-X (CaaX). The beta subunit is responsible for peptide-binding. Acts as an abscisic acid (ABA) negative regulator by mediating ASG2 farnesylation and consequently monitoring its subcellular localization. Involved in responses to salt (NaCl) and osmotic (e.g. in response to mannitol and PEG) stresses. KEYWORDS: Metal-binding;Prenyltransferase;Reference proteome;Repeat;Transferase;Zinc Q38944,"PROTEIN NAMES: Steroid 5-alpha-reductase DET2 ((22R,23R)-22,23-dihydroxycampest-4-en-3-one synthase) ((22S,24R)-22-hydroxy-5alpha-ergostan-3-one synthase) (Campest-3-one synthase) (Protein COMPACT ROSETTE 1) (Protein DEETIOLATED 2) (AtDET2) PROTEIN FAMILY: Steroid 5-alpha reductase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the steroid 5-alpha reductase family. FUNCTION: Involved in a reduction step in the biosynthesis of the plant steroid, brassinolide (BL); acts at the second step in brassinolide biosynthesis in the 5alpha-reduction of (24R)- 24-methylcholest-4-en-3-one, which is further modified to form campestanol. Can use progesterone, testosterone, androstenedione and campestenone as substrate. Catalyzes also the conversion of campest-4-en-3-one (campesta-4-en-3-one, 4-en-3-one) to campest-3-one (campesta-3-one, 3-one), of (22S,24R)-22-hydroxyergost-4-en-3-one (22-hydroxy-campesta-4-en-3-one, 22-OH-4-en-3-one) to (22S,24R)-22-hydroxy-5alpha-ergostan-3-one (22-hydroxy-campesta-3-one, 22-OH-3-one), and of (22R,23R)-22,23-dihydroxy-5alpha-campestan-3-one (22,23,diOH-4-en-3-one) to (22R,23R)-22,23-dihydroxycampest-4-en-3-one (6-deoxo3DT). Required for the brassinosteroid- (BR) dependent regulation of seed size and shape as well as embryo development. PATHWAY: Plant hormone biosynthesis; brassinosteroid biosynthesis. KEYWORDS: Brassinosteroid biosynthesis;Lipid biosynthesis;Lipid metabolism;Membrane;NADP;Oxidoreductase;Reference proteome;Steroid biosynthesis;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Multi-pass membrane protein " Q38954,"PROTEIN NAMES: Inorganic phosphate transporter 2-1, chloroplastic (H(+)/Pi cotransporter) (AtPht2;1) PROTEIN FAMILY: Inorganic phosphate transporter (PiT) family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20.2) family. FUNCTION: Low affinity H(+)/Pi chloroplastic cotransporter. Involved in inorganic phosphate (orthophosphate, Pi) uptake in green parts of plants in Pi-sufficient conditions. Required for Pi retranslocation during Pi deprivation. KEYWORDS: Alternative splicing;Chloroplast;Membrane;Phosphate transport;Plastid;Plastid inner membrane;Reference proteome;Symport;Transit peptide;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Plastid, chloroplast inner membrane ; Multi-pass membrane protein MISCELLANEOUS: [Isoform 2]: May be due to an intron retention." Q38BX0,"PROTEIN NAMES: Deoxyhypusine synthase PROTEIN FAMILY: Deoxyhypusine synthase family ORGANISM: Trypanosoma brucei brucei (strain 927/4 GUTat10.1) SIMILARITY: Belongs to the deoxyhypusine synthase family. FUNCTION: In association with the non-catalytic regulatory subunit DHSp, catalyzes the NAD-dependent oxidative cleavage of spermidine and the subsequent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the eIF5A precursor protein to form the intermediate deoxyhypusine residue. Regulates protein levels of its regulatory subunit DHSp. Required for cell growth and survival. PATHWAY: Protein modification; eIF5A hypusination. KEYWORDS: 3D-structure;Hypusine biosynthesis;Membrane;NAD;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Single-pass membrane protein " Q38CE9,"PROTEIN NAMES: ATP-dependent DNA helicase PIF6 (DNA repair and recombination helicase PIF6) PROTEIN FAMILY: Helicase family, PIF1 subfamily ORGANISM: Trypanosoma brucei brucei (strain 927/4 GUTat10.1) SIMILARITY: Belongs to the helicase family. PIF1 subfamily. FUNCTION: DNA-dependent ATPase and 5'-3' DNA helicase required for the maintenance of genome stability. KEYWORDS: ATP-binding;DNA damage;DNA recombination;DNA repair;DNA-binding;Helicase;Hydrolase;Nucleotide-binding;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus " Q39054,"PROTEIN NAMES: Molybdopterin biosynthesis protein CNX1 (Molybdenum cofactor biosynthesis enzyme CNX1) [Includes: Molybdopterin molybdenumtransferase (MPT Mo-transferase) (Domain E); Molybdopterin adenylyltransferase (MPT adenylyltransferase) (Domain G)] PROTEIN FAMILY: MoaB/Mog family; MoeA family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: In the N-terminal section; belongs to the MoaB/Mog family.; SIMILARITY: In the C-terminal section; belongs to the MoeA family. FUNCTION: Catalyzes two steps in the biosynthesis of the molybdenum cofactor. In the first step, molybdopterin is adenylated. Subsequently, molybdate is inserted into adenylated molybdopterin and AMP is released. PATHWAY: Cofactor biosynthesis; molybdopterin biosynthesis. KEYWORDS: 3D-structure;ATP-binding;Magnesium;Metal-binding;Molybdenum;Molybdenum cofactor biosynthesis;Multifunctional enzyme;Nucleotide-binding;Reference proteome;Transferase " Q39208,"PROTEIN NAMES: Delta(7)-sterol-C5(6)-desaturase 1 (Delta(7)-sterol-C5-desaturase 1) (Delta-7-C-5 sterol desaturase 1) (Protein DWARF 7) (Protein STEROL 1) PROTEIN FAMILY: Sterol desaturase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the sterol desaturase family. FUNCTION: Involved in the biosynthesis of sitosterol and campesterol, a precursor of growth-promoting brassinosteroids. KEYWORDS: Endoplasmic reticulum;Iron;Lipid biosynthesis;Lipid metabolism;Membrane;Oxidoreductase;Reference proteome;Steroid biosynthesis;Steroid metabolism;Sterol biosynthesis;Sterol metabolism;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein " Q39219,"PROTEIN NAMES: Ubiquinol oxidase 1a, mitochondrial (Alternative oxidase 1a) PROTEIN FAMILY: Alternative oxidase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the alternative oxidase family. FUNCTION: Catalyzes the cyanide-resistant oxidation of ubiquinol and the reduction of molecular oxygen to water, but does not translocate protons and consequently is not linked to oxidative phosphorylation. Increases respiration when the cytochrome respiratory pathway is restricted, or in response to low temperatures. KEYWORDS: Disulfide bond;Electron transport;Iron;Membrane;Metal-binding;Mitochondrion;Mitochondrion inner membrane;Oxidoreductase;Reference proteome;Respiratory chain;Transit peptide;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Mitochondrion inner membrane ; Multi-pass membrane protein Note=Mitochondrial, possibly in the inner surface of the inner mitochondrial membrane. MISCELLANEOUS: Cys-127 is involved in the sulfhydryl/disulfide regulation system, but is not required for subunit dimerization. Presence of a positive charge at this residue 127 confers activity while an uncharged substitution creates an inactive enzyme." Q39547,PROTEIN NAMES: Cucumisin (allergen Cuc m 1) PROTEIN FAMILY: Peptidase S8 family ORGANISM: Cucumis melo (Muskmelon) SIMILARITY: Belongs to the peptidase S8 family. KEYWORDS: 3D-structure;Allergen;Autocatalytic cleavage;Direct protein sequencing;Disulfide bond;Glycoprotein;Hydrolase;Protease;Reference proteome;Secreted;Serine protease;Signal;Zymogen SUBCELLULAR LOCATION: Secreted Q3E846,"PROTEIN NAMES: Cytochrome c oxidase assembly factor 6 PROTEIN FAMILY: Cytochrome c oxidase subunit 6B family ORGANISM: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIMILARITY: Belongs to the cytochrome c oxidase subunit 6B family. FUNCTION: Involved in the maturation of the mitochondrial respiratory chain complex IV subunit MT-CO2/COX2. Thereby, may regulate early steps of complex IV assembly. Mitochondrial respiratory chain complex IV or cytochrome c oxidase is the component of the respiratory chain that catalyzes the transfer of electrons from intermembrane space cytochrome c to molecular oxygen in the matrix and as a consequence contributes to the proton gradient involved in mitochondrial ATP synthesis. May also be required for efficient formation of respiratory supercomplexes comprised of complexes III and IV. KEYWORDS: Cytoplasm;Disulfide bond;Mitochondrion;Nucleus;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Nucleus Mitochondrion intermembrane space Note=Imported into the mitochondria via the mitochondrial MIA40-ERV1 machinery. MISCELLANEOUS: Present with 721 molecules/cell in log phase SD medium." Q3EBR6,"PROTEIN NAMES: Phospholipase A1-Igamma2, chloroplastic (DAD1-like lipase 3) PROTEIN FAMILY: AB hydrolase superfamily, Lipase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the AB hydrolase superfamily. Lipase family. FUNCTION: Acylhydrolase with broad specificity. Catalyzes the hydrolysis of phosphatidylcholine at the sn-1 position. Possesses moderate activity toward phosphatidylcholine (PC), monogalactosyldiacylglycerol (MGDG), digalactosyldiacylglycerol (DGDG) and triacylglycerol (TAG). KEYWORDS: Alternative splicing;Chloroplast;Hydrolase;Lipid degradation;Lipid metabolism;Plastid;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast MISCELLANEOUS: [Isoform 2]: Major isoform." Q3EDG5,PROTEIN NAMES: Protein-tyrosine sulfotransferase (Tyrosylprotein sulfotransferase) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Catalyzes the O-sulfation of tyrosine residues within acidic motifs of polypeptides. KEYWORDS: Alternative splicing;Glycoprotein;Golgi apparatus;Membrane;Reference proteome;Signal;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus membrane ; Single-pass type I membrane protein Q3IZ91,"PROTEIN NAMES: Crotonyl-CoA carboxylase/reductase PROTEIN FAMILY: Zinc-containing alcohol dehydrogenase family, Crotonyl-CoA carboxylase/reductase subfamily ORGANISM: Cereibacter sphaeroides (strain ATCC 17023 / DSM 158 / JCM 6121 / CCUG 31486 / LMG 2827 / NBRC 12203 / NCIMB 8253 / ATH 2.4.1.) (Rhodobacter sphaeroides) SIMILARITY: Belongs to the zinc-containing alcohol dehydrogenase family. Crotonyl-CoA carboxylase/reductase subfamily. FUNCTION: Catalyzes the NADPH-dependent reductive carboxylation of crotonyl-CoA ((2E)-butenoyl-CoA) to (2S)-ethylmalonyl-CoA, in the presence of CO2. This is a key reaction in the ethylmalonyl-CoA pathway for acetyl-CoA assimilation required for R.sphaeroides growth on acetate as sole carbon source. Is also able to accept acryloyl-CoA as an alternative substrate, yielding (2S)-methylmalonyl-CoA. To a lesser extent, when CO2 is absent, the enzyme also catalyzes the reduction of crotonyl-CoA to butanoyl-CoA. KEYWORDS: NADP;Oxidoreductase;Reference proteome " Q3MIT2,"PROTEIN NAMES: tRNA pseudouridine synthase Pus10 (Hup10) (Coiled-coil domain-containing protein 139) (tRNA pseudouridine 55 synthase) (Psi55 synthase) (tRNA pseudouridylate synthase) (tRNA-uridine isomerase) PROTEIN FAMILY: Pseudouridine synthase Pus10 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the pseudouridine synthase Pus10 family. FUNCTION: Protein with different functions depending on its subcellular location: involved in miRNA processing in the nucleus and acts as a tRNA pseudouridylate synthase in the cytoplasm. In the cytoplasm, acts as a pseudouridylate synthase by catalyzing synthesis of pseudouridine(54) and pseudouridine(55) from uracil-54 and uracil-55, respectively, in the psi GC loop of a subset of tRNAs. tRNA pseudouridylate synthase activity is enhanced by the presence of 1-methyladenosine at position 53-61 of tRNAs. Does not show tRNA pseudouridylate synthase activity in the nucleus. In the nucleus, promotes primary microRNAs (pri-miRNAs) processing independently of its RNA pseudouridylate synthase activity. Binds pri-miRNAs. Modulator of TRAIL/TNFSF10-induced cell death via activation of procaspase-8 and BID cleavage. Required for the progression of the apoptotic signal through intrinsic mitochondrial cell death. KEYWORDS: 3D-structure;Coiled coil;Cytoplasm;Isomerase;Metal-binding;Mitochondrion;Nucleus;Phosphoprotein;Reference proteome;tRNA processing;Zinc SUBCELLULAR LOCATION: Nucleus Cytoplasm Mitochondrion Note=Localizes mainly in the nucleus (Probable). tRNA pseudouridylate synthase activity is restricted to the cytoplasm. Translocates from nucleus to mitochondria during TRAIL-induced apoptosis. " Q3SXY8,"PROTEIN NAMES: ADP-ribosylation factor-like protein 13B (ADP-ribosylation factor-like protein 2-like 1) (ARL2-like protein 1) PROTEIN FAMILY: Small GTPase superfamily, Arf family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the small GTPase superfamily. Arf family. FUNCTION: Cilium-specific protein required to control the microtubule-based, ciliary axoneme structure. May act by maintaining the association between IFT subcomplexes A and B. Binds GTP but is not able to hydrolyze it; the GTPase activity remains unclear. Required to pattern the neural tube. Involved in cerebral cortex development: required for the initial formation of a polarized radial glial scaffold, the first step in the construction of the cerebral cortex, by regulating ciliary signaling. Regulates the migration and placement of postmitotic interneurons in the developing cerebral cortex. May regulate endocytic recycling traffic; however, additional evidence is required to confirm these data. KEYWORDS: Alternative splicing;Cell membrane;Cell projection;Ciliopathy;Cilium;Coiled coil;Disease variant;GTP-binding;Isopeptide bond;Joubert syndrome;Lipoprotein;Membrane;Nucleotide-binding;Palmitate;Reference proteome;Ubl conjugation SUBCELLULAR LOCATION: Cell projection, cilium membrane ; Lipid-anchor Cell projection, cilium Note=Associates to the cilium membrane via palmitoylation. Localizes to proximal ciliary membranes, to an inversin-like subciliary membrane compartment, excluding the transition zone. MISCELLANEOUS: Used as a ciliary marker because of its specific localization to microtubule doublets of the ciliary axoneme." Q3SY69,"PROTEIN NAMES: Mitochondrial 10-formyltetrahydrofolate dehydrogenase (Mitochondrial 10-FTHFDH) (mtFDH) (Aldehyde dehydrogenase family 1 member L2) PROTEIN FAMILY: GART family; Aldehyde dehydrogenase family, ALDH1L subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: In the N-terminal section; belongs to the GART family.; SIMILARITY: In the C-terminal section; belongs to the aldehyde dehydrogenase family. ALDH1L subfamily. FUNCTION: Mitochondrial 10-formyltetrahydrofolate dehydrogenase that catalyzes the NADP(+)-dependent conversion of 10-formyltetrahydrofolate to tetrahydrofolate and carbon dioxide. KEYWORDS: Acetylation;Alternative splicing;Mitochondrion;NADP;One-carbon metabolism;Oxidoreductase;Phosphopantetheine;Phosphoprotein;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Mitochondrion " Q3SYG4,"PROTEIN NAMES: Protein PTHB1 (Bardet-Biedl syndrome 9 protein) (Parathyroid hormone-responsive B1 gene protein) ORGANISM: Homo sapiens (Human) FUNCTION: The BBSome complex is thought to function as a coat complex required for sorting of specific membrane proteins to the primary cilia. The BBSome complex is required for ciliogenesis but is dispensable for centriolar satellite function. This ciliogenic function is mediated in part by the Rab8 GDP/GTP exchange factor, which localizes to the basal body and contacts the BBSome. Rab8(GTP) enters the primary cilium and promotes extension of the ciliary membrane. Firstly the BBSome associates with the ciliary membrane and binds to RAB3IP/Rabin8, the guanosyl exchange factor (GEF) for Rab8 and then the Rab8-GTP localizes to the cilium and promotes docking and fusion of carrier vesicles to the base of the ciliary membrane. Required for proper BBSome complex assembly and its ciliary localization. KEYWORDS: 3D-structure;Alternative splicing;Bardet-Biedl syndrome;Cell membrane;Cell projection;Chromosomal rearrangement;Ciliopathy;Cilium;Cilium biogenesis/degradation;Cytoplasm;Cytoskeleton;Disease variant;Intellectual disability;Membrane;Obesity;Protein transport;Reference proteome;Sensory transduction;Transport;Vision SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cell projection, cilium membrane. Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriolar satellite. " Q3T005,"PROTEIN NAMES: PDZ and LIM domain protein 4 ORGANISM: Bos taurus (Bovine) FUNCTION: Suppresses SRC activation by recognizing and binding to active SRC and facilitating PTPN13-mediated dephosphorylation of SRC 'Tyr-419' leading to its inactivation. Inactivated SRC dissociates from this protein allowing the initiation of a new SRC inactivation cycle. Involved in reorganization of the actin cytoskeleton (By similarity). In nonmuscle cells, binds to ACTN1 (alpha-actinin-1), increases the affinity of ACTN1 to F-actin (filamentous actin), and promotes formation of actin stress fibers. Involved in regulation of the synaptic AMPA receptor transport in dendritic spines of hippocampal pyramidal neurons directing the receptors toward an insertion at the postsynaptic membrane. Links endosomal surface-internalized GRIA1-containing AMPA receptors to the alpha-actinin/actin cytoskeleton. Increases AMPA receptor-mediated excitatory postsynaptic currents in neurons (By similarity). KEYWORDS: Cell projection;Cytoplasm;Cytoskeleton;Endosome;LIM domain;Membrane;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Synapse;Synaptosome;Zinc SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton Cell projection, dendritic spine Early endosome membrane ; Peripheral membrane protein ; Cytoplasmic side Recycling endosome membrane ; Peripheral membrane protein; Cytoplasmic side Nucleus Cytoplasm, perinuclear region Cell projection, lamellipodium Synapse, synaptosome Note=Localizes to actin stress fibers in nonmuscle cells. Colocalizes with GRIA1 in early endosomes. Enriched in numerous but not all spine-like structures along dendritic branches. Colocalizes with actin and enriched at sites containing larger amounts of actin and alpha-actinin. Targeted efficiently to spines via its PDZ domain-mediated interaction with the alpha-actinin/actin cytoskeletal complex. Localizes to synaptosomes in brain (By similarity). Colocalizes with F-actin. Colocalizes with TRIP6 at cell-cell contacts and lamellipodia. In the cytoplasm, displays a fibrillar pattern with characteristic thick fibers and occasional clusters. Colocalizes with the actin stress fibers. Oxidative stress induces redistribution from cytoskeleton to cytosol. Colocalizes with SRC at the perinuclear region, but not at focal adhesions (By similarity). " Q3T0D0,"PROTEIN NAMES: Heterogeneous nuclear ribonucleoprotein K (hnRNP K) ORGANISM: Bos taurus (Bovine) FUNCTION: One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA. Plays an important role in p53/TP53 response to DNA damage, acting at the level of both transcription activation and repression. When sumoylated, acts as a transcriptional coactivator of p53/TP53, playing a role in p21/CDKN1A and 14-3-3 sigma/SFN induction. As far as transcription repression is concerned, acts by interacting with long intergenic RNA p21 (lincRNA-p21), a non-coding RNA induced by p53/TP53. This interaction is necessary for the induction of apoptosis, but not cell cycle arrest (By similarity). As part of a ribonucleoprotein complex composed at least of ZNF827, HNRNPL and the circular RNA circZNF827 that nucleates the complex on chromatin, may negatively regulate the transcription of genes involved in neuronal differentiation (By similarity). KEYWORDS: Acetylation;Activator;Cell junction;Cell projection;Cytoplasm;DNA-binding;Glycoprotein;Isopeptide bond;Methylation;mRNA processing;mRNA splicing;Nucleus;Phosphoprotein;Reference proteome;Repeat;Repressor;Ribonucleoprotein;RNA-binding;Spliceosome;Transcription;Transcription regulation;Ubl conjugation SUBCELLULAR LOCATION: Cytoplasm Nucleus, nucleoplasm Cell projection, podosome " Q3T0T0,"PROTEIN NAMES: Glycerophosphodiester phosphodiesterase 1 (Glycerophosphoinositol glycerophosphodiesterase GDE1) (Lysophospholipase D GDE1) (Membrane-interacting protein of RGS16) PROTEIN FAMILY: Glycerophosphoryl diester phosphodiesterase family ORGANISM: Bos taurus (Bovine) SIMILARITY: Belongs to the glycerophosphoryl diester phosphodiesterase family. FUNCTION: Hydrolyzes the phosphodiester bond of glycerophosphodiesters such as glycerophosphoinositol (GroPIns) and glycerophosphoethanolamine (GroPEth), to yield a glycerol phosphate and an alcohol (By similarity). Hydrolyzes glycerophospho-N-acylethanolamines to N-acylethanolamines in the brain and participates in bioactive N-acylethanolamine biosynthesis such as anandamide (an endocannabinoid), N-palmitoylethanolamine (an anti-inflammatory), and N-oleoylethanolamine (an anorexic). In addition, has a lysophospholipase D activity by hydrolyzing N-acyl-lysoplasmenylethanolamine (N-acyl-lysoPlsEt) to N-acylethanolamine. However lysophospholipase D activity is lower than glycerophosphodiester phosphodiesterase activity (By similarity). Has little or no activity towards glycerophosphocholine (By similarity). KEYWORDS: Cell membrane;Cytoplasmic vesicle;Glycoprotein;Hydrolase;Lipid metabolism;Magnesium;Membrane;Metal-binding;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Cytoplasmic vesicle membrane ; Multi-pass membrane protein Note=Perinuclear vesicles and cell membrane. " Q3T905,PROTEIN NAMES: Carboxypeptidase B (CPBHz) PROTEIN FAMILY: Peptidase M14 family ORGANISM: Helicoverpa zea (Corn earworm moth) (Heliothis zea) SIMILARITY: Belongs to the peptidase M14 family. FUNCTION: Metalloprotease which cleaves a single amino acid from the C-terminal end of polypeptide chains. Shows a strong preference for peptides with a terminal lysine residue. KEYWORDS: 3D-structure;Carboxypeptidase;Disulfide bond;Hydrolase;Metal-binding;Metalloprotease;Protease;Secreted;Signal;Zinc SUBCELLULAR LOCATION: Secreted Q3TCN2,PROTEIN NAMES: Putative phospholipase B-like 2 (66.3 kDa protein) (76 kDa protein) (p76) (LAMA-like protein 2) (Lamina ancestor homolog 2) (Phospholipase B domain-containing protein 2) [Cleaved into: Putative phospholipase B-like 2 28 kDa form; Putative phospholipase B-like 2 40 kDa form; Putative phospholipase B-like 2 15 kDa form] PROTEIN FAMILY: Phospholipase B-like family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the phospholipase B-like family. FUNCTION: Putative phospholipase. KEYWORDS: 3D-structure;Alternative splicing;Disulfide bond;Glycoprotein;Hydrolase;Lipid degradation;Lipid metabolism;Lysosome;Reference proteome;Signal SUBCELLULAR LOCATION: Lysosome lumen Q3TDX8,"PROTEIN NAMES: Cytochrome b5 reductase 4 (Flavohemoprotein b5/b5R) (b5+b5R) (N-terminal cytochrome b5 and cytochrome b5 oxidoreductase domain-containing protein) (cb5/cb5R) PROTEIN FAMILY: Flavoprotein pyridine nucleotide cytochrome reductase family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. FUNCTION: NADH-cytochrome b5 reductase involved in endoplasmic reticulum stress response pathway. Plays a critical role in protecting pancreatic beta-cells against oxidant stress, possibly by protecting the cell from excess buildup of reactive oxygen species (ROS). KEYWORDS: Acetylation;Alternative splicing;Endoplasmic reticulum;FAD;Flavoprotein;Heme;Iron;Metal-binding;NAD;Oxidoreductase;Reference proteome SUBCELLULAR LOCATION: Endoplasmic reticulum. Note=Soluble protein. " Q3TEL6,PROTEIN NAMES: E3 ubiquitin ligase Rnf157 (RING finger protein 157) (RING-type E3 ubiquitin transferase Rnf157) ORGANISM: Mus musculus (Mouse) FUNCTION: E3 ubiquitin ligase that ubiquitinates APBB1 for its degradation by the proteasome and thus prevents apoptosis and promotes survival of neurons. Has a dual role in neurons as it is also required for dendrite growth and maintenance for which its ligase activity is not critical. May act as a scaffold molecule to regulate this process. Acts as a downstream effector of the interconnected PI3K and MAPK signaling pathways and thus participates in the regulation of the cell cycle (By similarity). KEYWORDS: Alternative splicing;Cytoplasm;Lipoprotein;Metal-binding;Myristate;Reference proteome;Transferase;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cytoplasm Q3TJD7,"PROTEIN NAMES: PDZ and LIM domain protein 7 (LIM mineralization protein) (LMP) (Protein enigma) ORGANISM: Mus musculus (Mouse) FUNCTION: May function as a scaffold on which the coordinated assembly of proteins can occur. May play a role as an adapter that, via its PDZ domain, localizes LIM-binding proteins to actin filaments of both skeletal muscle and nonmuscle tissues. Involved in both of the two fundamental mechanisms of bone formation, direct bone formation (e.g. embryonic flat bones mandible and cranium), and endochondral bone formation (e.g. embryonic long bone development). Plays a role during fracture repair. Involved in BMP6 signaling pathway (By similarity). KEYWORDS: Alternative splicing;Cytoplasm;Cytoskeleton;Developmental protein;Differentiation;LIM domain;Metal-binding;Methylation;Osteogenesis;Phosphoprotein;Reference proteome;Repeat;Zinc SUBCELLULAR LOCATION: Cytoplasm Cytoplasm, cytoskeleton Note=Colocalizes with RET to the cell periphery and in some cytoskeletal components. Colocalizes with TPM2 near the Z line in muscle. Colocalizes with TBX4 and TBX5 to actin filaments (By similarity). " Q3TWI9,"PROTEIN NAMES: CSC1-like protein 2 (Transmembrane protein 63B) PROTEIN FAMILY: CSC1 family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the CSC1 (TC 1.A.17) family. FUNCTION: Acts as an osmosensitive calcium-permeable cation channel. Mechanosensitive ion channel that converts mechanical stimuli into a flow of ion. Acts as an inner ear osmosensor, essential for normal hearing and survival of inner ear outer hair cells (OHCs). Mediates calcium-dependent regulatory volume decrease in OHCs which is necessary for their survival. Required for the maintenance of the morphological integrity of OHCs under hypotonic conditions. Mediates hypo-osmolarity-induced calcium influx, leading to activation of calcium-dependent potassium channels required for the maintenance of OHC morphology. KEYWORDS: Calcium;Cell membrane;Glycoprotein;Hearing;Ion channel;Ion transport;Membrane;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein " Q3U0B3,"PROTEIN NAMES: Dehydrogenase/reductase SDR family member 11 (17-beta-hydroxysteroid dehydrogenase) (3-beta-hydroxysteroid 3-dehydrogenase) (Estradiol 17-beta-dehydrogenase) (Short-chain dehydrogenase/reductase family 24C member 1) PROTEIN FAMILY: Short-chain dehydrogenases/reductases (SDR) family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FUNCTION: Catalyzes the conversion of the 17-keto group of estrone, 4- and 5-androstenes and 5-alpha-androstanes into their 17-beta-hydroxyl metabolites and the conversion of the 3-keto group of 3-, 3,17- and 3,20- diketosteroids into their 3-hydroxyl metabolites. Exhibits reductive 3-beta-hydroxysteroid dehydrogenase activity toward 5-beta-androstanes, 5-beta-pregnanes, 4-pregnenes and bile acids. May also reduce endogenous and exogenous alpha-dicarbonyl compounds and xenobiotic alicyclic ketones. PATHWAY: Steroid biosynthesis; estrogen biosynthesis. KEYWORDS: Lipid metabolism;NADP;Nucleotide-binding;Oxidoreductase;Reference proteome;Secreted;Signal;Steroid metabolism SUBCELLULAR LOCATION: Secreted " Q3U133,"PROTEIN NAMES: Zinc finger protein 746 PROTEIN FAMILY: Krueppel C2H2-type zinc-finger protein family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein family. FUNCTION: Transcription repressor that specifically binds to the 5'-TATTTT[T/G]-3' consensus sequence on promoters and repress transcription of PGC-1-alpha (PPARGC1A), thereby playing a role in regulation of neuron death. KEYWORDS: Coiled coil;Cytoplasm;DNA-binding;Isopeptide bond;Metal-binding;Nucleus;Reference proteome;Repeat;Repressor;Transcription;Transcription regulation;Ubl conjugation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cytoplasm Nucleus Note=Mainly localizes to the cytoplasm; probably translocates to the nucleus to repress selected genes. " Q3U507,"PROTEIN NAMES: Probable G-protein coupled receptor 174 PROTEIN FAMILY: G-protein coupled receptor 1 family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the G-protein coupled receptor 1 family. FUNCTION: G-protein-coupled receptor of lysophosphatidylserine (LysoPS) that plays different roles in immune response. Plays a negative role in regulatory T-cell accumulation and homeostasis. Under inflammatory conditions where LysoPS production increases, contributes to the down-regulation of regulatory T-cell activity to favor effector response. Mediates the suppression of IL-2 production in activated T-lymphocytes leading to inhibition of growth, proliferation and differentiation of T-cells. Mechanistically, acts via G(12)/G(13)-containing heterotrimeric G proteins to trigger elevated cyclic AMP levels and protein kinase A/PKA activity, which may in turn act to antagonize proximal TCR signaling. Plays an important role in the initial period of sepsis through the regulation of macrophage polarization and pro- and anti-inflammatory cytokine secretions. Upon testosterone treatment, acts as a receptor for CCL21 and subsequently triggers through G(q)-alpha and G(12)/G(13) proteins a calcium flux leading to chemotactic effects on activated B-cells. Signals via GNA13 and PKA to promote CD86 up-regulation by follicular B-cells. KEYWORDS: Cell membrane;Disulfide bond;G-protein coupled receptor;Glycoprotein;Membrane;Receptor;Reference proteome;Transducer;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. " Q3U6Q4,PROTEIN NAMES: Phosphoinositide 3-kinase regulatory subunit 6 (Phosphoinositide 3-kinase gamma adapter protein of 87 kDa) (p84 PI3K adapter protein) (p84 PIKAP) (p87 PI3K adapter protein) (p87PIKAP) ORGANISM: Mus musculus (Mouse) FUNCTION: Regulatory subunit of the PI3K gamma complex. Acts as an adapter to drive activation of PIK3CG by beta-gamma G protein dimers. The PIK3CG:PIK3R6 heterodimer is much less sensitive to beta-gamma G proteins than PIK3CG:PIK3R5 and its membrane recruitment and beta-gamma G protein dimer-dependent activation requires HRAS bound to PIK3CG. Recruits of the PI3K gamma complex to a PDE3B:RAPGEF3 signaling complex involved in angiogenesis; signaling seems to involve RRAS. KEYWORDS: 3D-structure;Alternative splicing;Angiogenesis;Cell membrane;Cytoplasm;Membrane;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Cell membrane ; Peripheral membrane protein Note=Translocated to the plasma membrane in a Ras-dependent manner. Q3U7U3,"PROTEIN NAMES: F-box only protein 7 ORGANISM: Mus musculus (Mouse) FUNCTION: Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins and plays a role in several biological processes such as cell cycle, cell proliferation, or maintenance of chromosome stability. Recognizes and ubiquitinates BIRC2 and the cell cycle regulator DLGAP5. Plays a role downstream of PINK1 in the clearance of damaged mitochondria via selective autophagy (mitophagy) by targeting PRKN to dysfunctional depolarized mitochondria. Promotes MFN1 ubiquitination. Mediates the ubiquitination and proteasomal degradation of UXT isoform 2, thereby impairing the NF-kappa-B signaling pathway. Inhibits NF-kappa-B pathway also by promoting the ubiquitinatioin of TRAF2 (By similarity). Affects the assembly state and activity of the proteasome in the cells including neurons by ubiquitinating the proteasomal subunit PSMA2 via 'Lys-63'-linked polyubiquitin chains. Promotes 'Lys-48'-linked polyubiquitination SIRT7, leading to the hydrogen peroxide-induced cell death (By similarity). PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Cytoplasm;Methylation;Mitochondrion;Nucleus;Reference proteome;Ubl conjugation pathway SUBCELLULAR LOCATION: Cytoplasm Nucleus Mitochondrion Cytoplasm, cytosol Note=Predominantly cytoplasmic. A minor proportion is detected in the nucleus. Relocates from the cytosol to depolarized mitochondria (By similarity). " Q3UCV8,"PROTEIN NAMES: Ubiquitin thioesterase otulin (Deubiquitinating enzyme otulin) (OTU domain-containing deubiquitinase with linear linkage specificity) (Ubiquitin thioesterase Gumby) PROTEIN FAMILY: Peptidase C65 family, Otulin subfamily ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the peptidase C65 family. Otulin subfamily. FUNCTION: Deubiquitinase that specifically removes linear ('Met-1'-linked) polyubiquitin chains to substrates and acts as a regulator of angiogenesis and innate immune response. Required during angiogenesis, craniofacial and neuronal development by regulating the canonical Wnt signaling together with the LUBAC complex. Acts as a negative regulator of NF-kappa-B by regulating the activity of the LUBAC complex (By similarity). OTULIN function is mainly restricted to homeostasis of the LUBAC complex: acts by removing 'Met-1'-linked autoubiquitination of the LUBAC complex, thereby preventing inactivation of the LUBAC complex. Acts as a key negative regulator of inflammation by restricting spontaneous inflammation and maintaining immune homeostasis. In myeloid cell, required to prevent unwarranted secretion of cytokines leading to inflammation and autoimmunity by restricting linear polyubiquitin formation. Plays a role in innate immune response by restricting linear polyubiquitin formation on LUBAC complex in response to NOD2 stimulation, probably to limit NOD2-dependent pro-inflammatory signaling (By similarity). KEYWORDS: Acetylation;Angiogenesis;Coiled coil;Cytoplasm;Hydrolase;Immunity;Innate immunity;Phosphoprotein;Protease;Reference proteome;Thiol protease;Ubl conjugation;Ubl conjugation pathway;Wnt signaling pathway SUBCELLULAR LOCATION: Cytoplasm " Q3ULA2,"PROTEIN NAMES: F-box/WD repeat-containing protein 1A (Beta-TrCP protein E3RS-IkappaB) (Beta-transducin repeat-containing protein) (Beta-TrCP) (E3RSIkappaB) (mE3RS-IkappaB) (F-box and WD repeats protein beta-TrCP) (HOS) (Ubiquitin ligase FWD1) (pIkappaB-E3 receptor subunit) ORGANISM: Mus musculus (Mouse) FUNCTION: Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes and binds to phosphorylated target proteins. SCF(BTRC) mediates the ubiquitination of phosphorylated NFKB, ATF4, CDC25A, DLG1, FBXO5, PER1, SMAD3, SMAD4, SNAI1 and probably NFKB2. SCF(BTRC) mediates the ubiquitination of CTNNB1 and participates in Wnt signaling (By similarity). SCF(BTRC) mediates the ubiquitination of NFKBIA, NFKBIB and NFKBIE; the degradation frees the associated NFKB1 to translocate into the nucleus and to activate transcription. Ubiquitination of NFKBIA occurs at 'Lys-21' and 'Lys-22'. The SCF(FBXW11) complex also regulates NF-kappa-B by mediating ubiquitination of phosphorylated NFKB1: specifically ubiquitinates the p105 form of NFKB1, leading to its degradation (By similarity). SCF(BTRC) mediates the ubiquitination of CEP68; this is required for centriole separation during mitosis (By similarity). SCF(BTRC) mediates the ubiquitination and subsequent degradation of nuclear NFE2L1. Has an essential role in the control of the clock-dependent transcription via degradation of phosphorylated PER1 and PER2. May be involved in ubiquitination and subsequent proteasomal degradation through a DBB1-CUL4 E3 ubiquitin-protein ligase (By similarity). Required for activation of NFKB-mediated transcription by IL1B, MAP3K14, MAP3K1, IKBKB and TNF (By similarity). Required for proteolytic processing of GLI3. Mediates ubiquitination of REST, thereby leading to its proteasomal degradation (By similarity). SCF(BTRC) mediates the ubiquitination and subsequent proteasomal degradation of KLF4; thereby negatively regulating cell pluripotency maintenance and embryogenesis. SCF(BTRC) acts as a regulator of mTORC1 signaling pathway by catalyzing ubiquitination and subsequent proteasomal degradation of phosphorylated DEPTOR, TFE3 and MITF (By similarity). PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Alternative splicing;Biological rhythms;Cytoplasm;Nucleus;Reference proteome;Repeat;Ubl conjugation;Ubl conjugation pathway;WD repeat;Wnt signaling pathway SUBCELLULAR LOCATION: Cytoplasm Nucleus " Q3ULW8,"PROTEIN NAMES: Protein mono-ADP-ribosyltransferase PARP3 (ADP-ribosyltransferase diphtheria toxin-like 3) (ARTD3) (DNA ADP-ribosyltransferase PARP3) (NAD(+) ADP-ribosyltransferase 3) (ADPRT-3) (Poly [ADP-ribose] polymerase 3) (PARP-3) (Poly[ADP-ribose] synthase 3) (pADPRT-3) PROTEIN FAMILY: ARTD/PARP family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the ARTD/PARP family. FUNCTION: Mono-ADP-ribosyltransferase that mediates mono-ADP-ribosylation of target proteins and plays a key role in the response to DNA damage. Mediates mono-ADP-ribosylation of glutamate, aspartate or lysine residues on target proteins (By similarity). In contrast to PARP1 and PARP2, it is not able to mediate poly-ADP-ribosylation (By similarity). Involved in DNA repair by mediating mono-ADP-ribosylation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism, such as histone H2B, XRCC5 and XRCC6 (By similarity). ADP-ribosylation follows DNA damage and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks (By similarity). Involved in single-strand break repair by catalyzing mono-ADP-ribosylation of histone H2B on 'Glu-2' (H2BE2ADPr) of nucleosomes containing nicked DNA (By similarity). Cooperates with the XRCC5-XRCC6 (Ku80-Ku70) heterodimer to limit end-resection thereby promoting accurate NHEJ (By similarity). Suppresses G-quadruplex (G4) structures in response to DNA damage (By similarity). Associates with a number of DNA repair factors and is involved in the response to exogenous and endogenous DNA strand breaks. Together with APLF, promotes the retention of the LIG4-XRCC4 complex on chromatin and accelerate DNA ligation during non-homologous end-joining (NHEJ) (By similarity). May link the DNA damage surveillance network to the mitotic fidelity checkpoint (By similarity). Acts as a negative regulator of immunoglobulin class switch recombination, probably by controlling the level of AICDA /AID on the chromatin. In addition to proteins, also able to ADP-ribosylate DNA: mediates DNA mono-ADP-ribosylation of DNA strand break termini via covalent addition of a single ADP-ribose moiety to a 5'- or 3'-terminal phosphate residues in DNA containing multiple strand breaks (By similarity). KEYWORDS: ADP-ribosylation;Alternative splicing;Chromosome;Cytoplasm;Cytoskeleton;DNA damage;DNA repair;Glycosyltransferase;NAD;Nucleotidyltransferase;Nucleus;Reference proteome;Transferase SUBCELLULAR LOCATION: Nucleus Chromosome Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole Note=Almost exclusively localized in the nucleus and appears in numerous small foci and a small number of larger foci whereas a centrosomal location has not been detected. In response to DNA damage, localizes to sites of double-strand break. Also localizes to single-strand breaks. Preferentially localized to the daughter centriole. " Q3UMY5,"PROTEIN NAMES: Echinoderm microtubule-associated protein-like 4 (EMAP-4) PROTEIN FAMILY: WD repeat EMAP family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the WD repeat EMAP family. FUNCTION: Essential for the stability of microtubules (MTs). Essential for the formation of MTs (By similarity). Required for the organization of the mitotic spindle and for the proper attachment of kinetochores to MTs (By similarity). Promotes the recruitment of NUDC to the mitotic spindle for mitotic progression (By similarity). KEYWORDS: Acetylation;Alternative splicing;Cell cycle;Cell division;Coiled coil;Cytoplasm;Cytoskeleton;Microtubule;Mitosis;Phosphoprotein;Reference proteome;Repeat;WD repeat SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton Cytoplasm, cytoskeleton, spindle Cytoplasm Cytoplasm, cytoskeleton, microtubule organizing center Midbody Note=Localizes to microtubules (MTs) during interphase with a significantly reduced affinity for MTs during mitosis. " Q3UPF5,"PROTEIN NAMES: Zinc finger CCCH-type antiviral protein 1 (ADP-ribosyltransferase diphtheria toxin-like 13) (ARTD13) (Inactive Poly [ADP-ribose] polymerase 13) (PARP13) PROTEIN FAMILY: ARTD/PARP family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the ARTD/PARP family. FUNCTION: Antiviral protein which inhibits the replication of viruses by recruiting the cellular RNA degradation machineries to degrade the viral mRNAs. Binds to a ZAP-responsive element (ZRE) present in the target viral mRNA, recruits cellular poly(A)-specific ribonuclease PARN to remove the poly(A) tail, and the 3'-5' exoribonuclease complex exosome to degrade the RNA body from the 3'-end. It also recruits the decapping complex DCP1-DCP2 through RNA helicase p72 (DDX17) to remove the cap structure of the viral mRNA to initiate its degradation from the 5'-end. Its target viruses belong to families which include retroviridae: human immunodeficiency virus type 1 (HIV-1) and moloney and murine leukemia virus (MoMLV), filoviridae: ebola virus (EBOV) and marburg virus (MARV), togaviridae: sindbis virus (SINV) and Ross river virus (RRV). Specifically targets the multiply spliced but not unspliced or singly spliced HIV-1 mRNAs for degradation. Isoform 1 is a more potent viral inhibitor than isoform 2. Isoform 2 acts as a positive regulator of RIG-I signaling resulting in activation of the downstream effector IRF3 leading to the expression of type I IFNs and IFN stimulated genes (ISGs). KEYWORDS: 3D-structure;Alternative splicing;Antiviral defense;Cytoplasm;Immunity;Innate immunity;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Repeat;RNA-binding;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cytoplasm Nucleus Note=Localizes in the cytoplasm at steady state, but shuttles between nucleus and cytoplasm in a XPO1-dependent manner. " Q3UUY6,"PROTEIN NAMES: Prominin-2 (PROM-2) (Prominin-like protein 2) (mPROML2) PROTEIN FAMILY: Prominin family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the prominin family. KEYWORDS: Alternative splicing;Cell membrane;Cell projection;Cilium;Coiled coil;Glycoprotein;Membrane;Phosphoprotein;Reference proteome;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Apical cell membrane; Multi-pass membrane protein. Basolateral cell membrane; Multi-pass membrane protein. Cell projection, microvillus membrane; Multi-pass membrane protein. Cell projection, cilium membrane; Multi-pass membrane protein. Note=Colocalizes with PROM1 (By similarity). Associates with membrane in a cholesterol-dependent manner. Localizes to the apical and basolateral membranes of epithelial cells. " Q3UV55,"PROTEIN NAMES: Nuclear receptor subfamily 1 group D member 1 (Rev-erbA-alpha) (V-erbA-related protein 1) (EAR-1) PROTEIN FAMILY: Nuclear hormone receptor family, NR1 subfamily ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the nuclear hormone receptor family. NR1 subfamily. FUNCTION: Transcriptional repressor which coordinates circadian rhythm and metabolic pathways in a heme-dependent manner. Integral component of the complex transcription machinery that governs circadian rhythmicity and forms a critical negative limb of the circadian clock by directly repressing the expression of core clock components BMAL1, CLOCK and CRY1. Also regulates genes involved in metabolic functions, including lipid and bile acid metabolism, adipogenesis, gluconeogenesis and the macrophage inflammatory response. Acts as a receptor for heme which stimulates its interaction with the NCOR1/HDAC3 corepressor complex, enhancing transcriptional repression. Recognizes two classes of DNA response elements within the promoter of its target genes and can bind to DNA as either monomers or homodimers, depending on the nature of the response element. Binds as a monomer to a response element composed of the consensus half-site motif 5'-[A/G]GGTCA-3' preceded by an A/T-rich 5' sequence (RevRE), or as a homodimer to a direct repeat of the core motif spaced by two nucleotides (RevDR-2). Acts as a potent competitive repressor of ROR alpha (RORA) function and regulates the levels of its ligand heme by repressing the expression of PPARGC1A, a potent inducer of heme synthesis. Regulates lipid metabolism by repressing the expression of APOC3 and by influencing the activity of sterol response element binding proteins (SREBPs); represses INSIG2 which interferes with the proteolytic activation of SREBPs which in turn govern the rhythmic expression of enzymes with key functions in sterol and fatty acid synthesis. Regulates gluconeogenesis via repression of G6PC1 and PEPCK and adipocyte differentiation via repression of PPARG. Regulates glucagon release in pancreatic alpha-cells via the AMPK-NAMPT-SIRT1 pathway and the proliferation, glucose-induced insulin secretion and expression of key lipogenic genes in pancreatic-beta cells. Positively regulates bile acid synthesis by increasing hepatic expression of CYP7A1 via repression of NR0B2 and NFIL3 which are negative regulators of CYP7A1. Modulates skeletal muscle oxidative capacity by regulating mitochondrial biogenesis and autophagy; controls mitochondrial biogenesis and respiration by interfering with the STK11-PRKAA1/2-SIRT1-PPARGC1A signaling pathway. Represses the expression of SERPINE1/PAI1, an important modulator of cardiovascular disease and the expression of inflammatory cytokines and chemokines in macrophages. Represses gene expression at a distance in macrophages by inhibiting the transcription of enhancer-derived RNAs (eRNAs). Plays a role in the circadian regulation of body temperature and negatively regulates thermogenic transcriptional programs in brown adipose tissue (BAT); imposes a circadian oscillation in BAT activity, increasing body temperature when awake and depressing thermogenesis during sleep. In concert with NR2E3, regulates transcriptional networks critical for photoreceptor development and function. In addition to its activity as a repressor, can also act as a transcriptional activator. In the ovarian granulosa cells acts as a transcriptional activator of STAR which plays a role in steroid biosynthesis. In collaboration with SP1, activates GJA1 transcription in a heme-independent manner. Represses the transcription of CYP2B10, CYP4A10 and CYP4A14. Represses the transcription of CES2. Represses and regulates the circadian expression of TSHB in a NCOR1-dependent manner. Negatively regulates the protein stability of NR3C1 and influences the time-dependent subcellular distribution of NR3C1, thereby affecting its transcriptional regulatory activity. Plays a critical role in the circadian control of neutrophilic inflammation in the lung; under resting, non-stress conditions, acts as a rhythmic repressor to limit inflammatory activity whereas in the presence of inflammatory triggers undergoes ubiquitin-mediated degradation thereby relieving inhibition of the inflammatory response. Plays a key role in the circadian regulation of microglial activation and neuroinflammation; suppresses microglial activation through the NF-kappaB pathway in the central nervous system. Plays a role in the regulation of the diurnal rhythms of lipid and protein metabolism in the skeletal muscle via transcriptional repression of genes controlling lipid and amino acid metabolism in the muscle. KEYWORDS: Acetylation;Activator;Biological rhythms;Cell projection;Cytoplasm;Differentiation;DNA-binding;Heme;Iron;Metal-binding;Nucleus;Phosphoprotein;Receptor;Reference proteome;Repressor;Synapse;Transcription;Transcription regulation;Ubl conjugation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Cytoplasm Cell projection, dendrite Cell projection, dendritic spine Note=Localizes to the cytoplasm, dendrites and dendritic spine in the presence of OPHN1. Localizes predominantly to the nucleus at ZT8 whereas it is cytoplasmic at ZT20. Phosphorylation by CSNK1E enhances its cytoplasmic localization. " Q3UYG8,"PROTEIN NAMES: ADP-ribose glycohydrolase MACROD2 (MACRO domain-containing protein 2) (O-acetyl-ADP-ribose deacetylase MACROD2) ([Protein ADP-ribosylaspartate] hydrolase MACROD2) ([Protein ADP-ribosylglutamate] hydrolase MACROD2) ORGANISM: Mus musculus (Mouse) FUNCTION: Removes ADP-ribose from aspartate and glutamate residues in proteins bearing a single ADP-ribose moiety. Inactive towards proteins bearing poly-ADP-ribose. Deacetylates O-acetyl-ADP ribose, a signaling molecule generated by the deacetylation of acetylated lysine residues in histones and other proteins. KEYWORDS: DNA damage;Hydrolase;Isopeptide bond;Nucleus;Reference proteome;Ubl conjugation SUBCELLULAR LOCATION: Nucleus Note=Recruited to DNA lesions, probably via mono-APD-ribosylated proteins. " Q3UZD5,"PROTEIN NAMES: Putative histone-lysine N-methyltransferase PRDM6 (PR domain zinc finger protein 6) (PR domain-containing protein 6) (PR domain-containing protein in smooth muscle) PROTEIN FAMILY: Class V-like SAM-binding methyltransferase superfamily ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the class V-like SAM-binding methyltransferase superfamily. FUNCTION: Putative histone methyltransferase that acts as a transcriptional repressor of smooth muscle gene expression. Promotes the transition from differentiated to proliferative smooth muscle by suppressing differentiation and maintaining the proliferative potential of vascular smooth muscle cells. Also plays a role in endothelial cells by inhibiting endothelial cell proliferation, survival and differentiation. It is unclear whether it has histone methyltransferase activity in vivo. According to some authors, it does not act as a histone methyltransferase by itself and represses transcription by recruiting EHMT2/G9a. According to others, it possesses histone methyltransferase activity when associated with other proteins and specifically methylates 'Lys-20' of histone H4 in vitro. 'Lys-20' methylation represents a specific tag for epigenetic transcriptional repression. KEYWORDS: Alternative splicing;Chromatin regulator;Metal-binding;Methyltransferase;Nucleus;Reference proteome;Repeat;Repressor;S-adenosyl-L-methionine;Transcription;Transcription regulation;Transferase;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus " Q3V0J1,"PROTEIN NAMES: Transmembrane protein 237 (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 4 protein homolog) PROTEIN FAMILY: TMEM237 family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the TMEM237 family. FUNCTION: Component of the transition zone in primary cilia. Required for ciliogenesis. KEYWORDS: Alternative splicing;Cell projection;Cilium;Cilium biogenesis/degradation;Membrane;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Multi-pass membrane protein Cell projection, cilium Note=Localizes at the proximal region of primary cilia were observed, consistent with localization to the transition zone. " Q3Y416,"PROTEIN NAMES: Calcium/calmodulin-dependent protein kinase kinase (CaM-KK) (CaM-kinase kinase) PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. FUNCTION: Calcium/calmodulin-dependent protein kinase which phosphorylates cmk-1. Component of a calcium-triggered signaling cascade involved in CRE-mediated transcriptional activation, probably through cmk-1-mediated crh-1/CREB phosphorylation. KEYWORDS: 3D-structure;Alternative splicing;ATP-binding;Calmodulin-binding;Kinase;Magnesium;Metal-binding;Nucleotide-binding;Reference proteome;Serine/threonine-protein kinase;Transferase " Q3Y6B8,PROTEIN NAMES: CAAX prenyl protease 1 homolog (Zinc metalloproteinase Ste24 homolog) PROTEIN FAMILY: Peptidase M48A family ORGANISM: Taenia solium (Pork tapeworm) SIMILARITY: Belongs to the peptidase M48A family. FUNCTION: Zinc-dependent metalloproteinase. Proteolytically removes the C-terminal three residues of farnesylated proteins (By similarity). KEYWORDS: Disulfide bond;Endoplasmic reticulum;Hydrolase;Membrane;Metal-binding;Metalloprotease;Protease;Transmembrane;Transmembrane helix;Zinc SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein Q3YBM2,PROTEIN NAMES: Transmembrane protein 176B (Protein LR8) PROTEIN FAMILY: TMEM176 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the TMEM176 family. FUNCTION: May play a role in the process of maturation of dendritic cells. Required for the development of cerebellar granule cells (By similarity). KEYWORDS: Alternative splicing;Differentiation;Membrane;Nucleus;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Nucleus membrane ; Multi-pass membrane protein Q3YBR2,"PROTEIN NAMES: Transforming growth factor beta regulator 1 (Nuclear interactor of ARF and Mdm2) PROTEIN FAMILY: TBRG1 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the TBRG1 family. FUNCTION: Acts as a growth inhibitor. Can activate p53/TP53, causes G1 arrest and collaborates with CDKN2A to restrict proliferation, but does not require either protein to inhibit DNA synthesis. Redistributes CDKN2A into the nucleoplasm. Involved in maintaining chromosomal stability. KEYWORDS: 3D-structure;Acetylation;Alternative splicing;Cell cycle;Nucleus;Phosphoprotein;Reference proteome;Tumor suppressor;Ubl conjugation SUBCELLULAR LOCATION: Nucleus " Q3ZAV1,"PROTEIN NAMES: Solute carrier family 22 member 12 (Urate anion exchanger 1) (URAT1) (Urate:anion antiporter SLC22A12) PROTEIN FAMILY: Major facilitator superfamily, Organic cation transporter family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the major facilitator (TC 2.A.1) superfamily. Organic cation transporter (TC 2.A.1.19) family. FUNCTION: Electroneutral antiporter that translocates urate across the apical membrane of proximal tubular cells in exchange for monovalent organic or inorganic anions. Involved in renal reabsorption of urate and helps maintaining blood levels of uric acid. Mediates urate uptake by an exchange with organic anions such as (S)-lactate and nicotinate, and inorganic anion Cl(-). Other inorganic anions such as Br(-), I(-) and NO3(-) may also act as counteranions that exchange for urate (By similarity). Also mediates orotate tubular uptake coupled with nicotinate efflux and to a lesser extent with lactate efflux, therefore displaying a potential role in orotate renal reabsorption. Orotate transport is Cl(-)-dependent (By similarity). KEYWORDS: Cell membrane;Glycoprotein;Ion transport;Membrane;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Apical cell membrane ; Multi-pass membrane protein " Q40541,"PROTEIN NAMES: Mitogen-activated protein kinase kinase kinase NPK1 (Nicotiana protein kinase 1) PROTEIN FAMILY: Protein kinase superfamily, STE Ser/Thr protein kinase family, MAP kinase kinase kinase subfamily ORGANISM: Nicotiana tabacum (Common tobacco) SIMILARITY: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily. FUNCTION: Functions in the NACK-PQR (NPK1-NQK1/MEK1-NRK1) MAP kinase signaling pathway, which is essential for somatic cell cytokinesis, especially for the cell-plate formation and its expansion, and depends on NACK1 and NACK2 kinesin-related proteins. Functions in the regulation of resistance gene-mediated resistance responses such as the N-mediated resistance to tobamovirus (TMV) and the Rx-mediated hypersensitive response (HR) to potato virus X (PVX). KEYWORDS: ATP-binding;Cell cycle;Cell division;Cytoplasm;Cytoskeleton;Kinase;Nucleotide-binding;Nucleus;Phosphoprotein;Plant defense;Reference proteome;Serine/threonine-protein kinase;Transferase SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, phragmoplast. Note=In interphase and prophase, detected in the nucleus. From prometaphase to metaphase, found in the cytoplasm in patches. At anaphase, distributed around the spindle midzone and on the phragmoplast equator at telophase and then redistributed to the newly forming daughter nuclei. MISCELLANEOUS: Plants overexpressing the NPK1 kinase domain produced seeds defective in embryo and endosperm development. Expression of kinase-negative NPK1 causes inhibition of phragmoplast expansion and multinucleate cells. Plants silencing NPK1 exhibit reduced cell size, defective cytokinesis and an overall dwarf phenotype." Q42545,"PROTEIN NAMES: Cell division protein FtsZ homolog 1, chloroplastic (AtFtsZ1) (AtFtsZ1-1) (Chloroplast FtsZ) (CpFtsZ) (Protein ACCUMULATION AND REPLICATION OF CHLOROPLASTS 10) (Protein PLASTID MOVEMENT IMPAIRED4) PROTEIN FAMILY: FtsZ family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the FtsZ family. FUNCTION: Exhibits GTPase activity. Component of the plastid division machinery that forms a contractile ring at the division site. Required for plastid division in a dose-dependent manner. Involved in epidermal plastids division in a MINE1-dependent manner. Involved in blue light-induced chloroplast movements. May regulate thylakoid development. In the vegetative shoot apex, at the shoot apical meristem (SAM), where the proplastid-to-chloroplast transition takes place, contributes equally with FTSZ2-1 in the L2 layer to plastid division. KEYWORDS: Acetylation;Cell cycle;Chloroplast;GTP-binding;Membrane;Nucleotide-binding;Plastid;Reference proteome;Thylakoid;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast stroma Plastid, chloroplast thylakoid membrane ; Peripheral membrane protein. Note=Forms a contractile ring at the chloroplast midpoint that coaligns with FTSZ2-1 rings. Exhibits a dynamic trunover in FtsZ ring facilitated by ARC3-mediated destabilization. " Q43157,"PROTEIN NAMES: Ribulose-phosphate 3-epimerase, chloroplastic (Pentose-5-phosphate 3-epimerase) (PPE) (R5P3E) (RPE) PROTEIN FAMILY: Ribulose-phosphate 3-epimerase family ORGANISM: Spinacia oleracea (Spinach) SIMILARITY: Belongs to the ribulose-phosphate 3-epimerase family. FUNCTION: Catalyzes the reversible epimerization of D-ribulose 5-phosphate to D-xylulose 5-phosphate. PATHWAY: Carbohydrate biosynthesis; Calvin cycle. KEYWORDS: Calvin cycle;Carbohydrate metabolism;Chloroplast;Cobalt;Direct protein sequencing;Iron;Isomerase;Manganese;Membrane;Metal-binding;Pentose shunt;Plastid;Reference proteome;Thylakoid;Transit peptide;Zinc SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane " Q45588,"PROTEIN NAMES: Anti-sigma-W factor RsiW (Regulator of SigW) (Sigma-W anti-sigma factor RsiW) PROTEIN FAMILY: Zinc-associated anti-sigma factor (ZAS) superfamily, Anti-sigma-W factor family ORGANISM: Bacillus subtilis (strain 168) SIMILARITY: Belongs to the zinc-associated anti-sigma factor (ZAS) superfamily. Anti-sigma-W factor family. FUNCTION: The anti-sigma factor for extracytoplasmic function (ECF) sigma factor sigma-W (SigW). Holds SigW, its cognate ECF sigma factor, in an inactive form until released by regulated intramembrane proteolysis (RIP). SigW and RsiW mediate cell response to cell wall stress. RIP occurs when an extracytoplasmic signal triggers a concerted proteolytic cascade to transmit information and elicit cellular responses. The membrane-spanning regulatory substrate protein is first cut periplasmically (site-1 protease, S1P, PrsW) , then within the membrane itself (site-2 protease, S2P, RasP) , while cytoplasmic proteases finish degrading the anti-sigma factor, liberating sigma-W. KEYWORDS: 3D-structure;Cell membrane;Membrane;Metal-binding;Reference proteome;Stress response;Transcription;Transcription regulation;Transmembrane;Transmembrane helix;Zinc SUBCELLULAR LOCATION: Cell membrane ; Single-pass membrane protein Note=Site-2 clipped RsiW is released from the membrane to the cytoplasm. " Q460N3,PROTEIN NAMES: Protein mono-ADP-ribosyltransferase PARP15 (ADP-ribosyltransferase diphtheria toxin-like 7) (ARTD7) (B-aggressive lymphoma protein 3) (Poly [ADP-ribose] polymerase 15) (PARP-15) PROTEIN FAMILY: ARTD/PARP family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the ARTD/PARP family. FUNCTION: Mono-ADP-ribosyltransferase that mediates mono-ADP-ribosylation of target proteins. Acts as a negative regulator of transcription. KEYWORDS: 3D-structure;Alternative splicing;Glycosyltransferase;NAD;Nucleotidyltransferase;Nucleus;Reference proteome;Repeat;Transcription;Transcription regulation;Transferase SUBCELLULAR LOCATION: Nucleus Q46222,"PROTEIN NAMES: 3-deoxy-D-manno-octulosonic acid transferase (Kdo transferase) (Kdo(2)-lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase) (Kdo-lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase) (Lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase) (Trifunctional Kdo transferase) PROTEIN FAMILY: Glycosyltransferase group 1 family, Glycosyltransferase 30 subfamily ORGANISM: Chlamydia pneumoniae (Chlamydophila pneumoniae) SIMILARITY: Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 30 subfamily. FUNCTION: Involved in lipopolysaccharide (LPS) biosynthesis. Catalyzes the transfer of three 3-deoxy-D-manno-octulosonate (Kdo) residues from CMP-Kdo to lipid IV(A), the tetraacyldisaccharide-1,4'-bisphosphate precursor of lipid A. Thus generates the genus-specific LPS epitope of Chlamydia, composed of the trisaccharide alpha-Kdo-(2->8)-alpha-Kdo-(2->4)-alpha-Kdo. PATHWAY: Bacterial outer membrane biogenesis; LPS core biosynthesis. KEYWORDS: Cell inner membrane;Cell membrane;Lipopolysaccharide biosynthesis;Membrane;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell inner membrane ; Single-pass membrane protein ; Cytoplasmic side " Q46389,PROTEIN NAMES: 5-methyltetrahydrofolate:corrinoid/iron-sulfur protein co-methyltransferase (5-methyltetrahydrofolate corrinoid/iron sulfur protein methyltransferase) (MeTr) PROTEIN FAMILY: Vitamin-B12 dependent methionine synthase family ORGANISM: Moorella thermoacetica (Clostridium thermoaceticum) SIMILARITY: Belongs to the vitamin-B12 dependent methionine synthase family. FUNCTION: Methyltransferase that mediates the transfer of a N5-methyl group of (6S)-methyltetrahydrofolate to the 5-methoxybenzimidazolylcobamide cofactor of a corrinoid/Fe-S protein (AcsC/AcsD) in the anaerobic acetyl-CoA pathway (Wood-Ljungdahl pathway) of carbon monoxide and carbon dioxide fixation. KEYWORDS: 3D-structure;Calcium;Carbon dioxide fixation;Cobalamin;Cobalt;Metal-binding;Methyltransferase;Transferase Q46444,PROTEIN NAMES: Quinohemoprotein alcohol dehydrogenase (QH-ADH) (Alcohol dehydrogenase (azurin)) (PQQ-containing alcohol dehydrogenase) (PQQ-dependent ADH) (Quinohaemoprotein ethanol dehydrogenase type I) (QH-EDHI) PROTEIN FAMILY: Bacterial PQQ dehydrogenase family ORGANISM: Comamonas testosteroni (Pseudomonas testosteroni) SIMILARITY: Belongs to the bacterial PQQ dehydrogenase family. FUNCTION: Catalyzes the dye-linked oxidation of primary alcohols to the corresponding aldehydes and the (subsequent) oxidation of the aldehydes to carboxylic acids. Methanol is not a substrate. KEYWORDS: 3D-structure;Calcium;Direct protein sequencing;Disulfide bond;Heme;Iron;Metal-binding;Oxidoreductase;Periplasm;PQQ;Signal SUBCELLULAR LOCATION: Periplasm Q46821,PROTEIN NAMES: Uric acid transporter UacT PROTEIN FAMILY: Nucleobase:cation symporter-2 (NCS2) family ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) family. FUNCTION: Proton-dependent high-capacity transporter for uric acid. Shows also a low capacity for transport of xanthine at 37 degrees Celsius but not at 25 degrees Celsius. KEYWORDS: Cell inner membrane;Cell membrane;Hydrogen ion transport;Ion transport;Membrane;Reference proteome;Symport;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell inner membrane ; Multi-pass membrane protein Q46897,"PROTEIN NAMES: CRISPR system Cascade subunit CasE (CasE endoRNase) (crRNA endonuclease) PROTEIN FAMILY: CRISPR-associated protein Cas6/Cse3/CasE family, Subtype I-E/Ecoli subfamily ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the CRISPR-associated protein Cas6/Cse3/CasE family. Subtype I-E/Ecoli subfamily. FUNCTION: CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA).; FUNCTION: CasE is required to process the pre-crRNA into single repeat-spacer units, with an 8-nt 5'-repeat DNA tag that may help other proteins recognize the crRNA. This subunit alone will cleave pre-crRNA, as will CasCDE or CasCE; cleavage does not require divalent metals or ATP. CasCDE alone is also able to form R-loops. Partially inhibits the cleavage of Holliday junctions by YgbT (Cas1). Yields a 5'-hydroxy group and a 2',3'-cyclic phosphate terminus.; FUNCTION: A component of Cascade, which participates in CRISPR interference, the third stage of CRISPR immunity. Cascade binds both crRNA and in a sequence-specific manner negatively supercoiled dsDNA target. This leads to the formation of an R-loop in which the crRNA binds the target DNA, displacing the noncomplementary strand. Cas3 is recruited to Cascade, nicks target DNA and then unwinds and cleaves the target, leading to DNA degradation and invader neutralization. KEYWORDS: 3D-structure;Antiviral defense;Endonuclease;Hydrolase;Nuclease;Reference proteome;RNA-binding " Q46898,"PROTEIN NAMES: CRISPR system Cascade subunit CasD PROTEIN FAMILY: CRISPR-associated protein Cas5 family, Subtype I-E/Ecoli subfamily ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the CRISPR-associated protein Cas5 family. Subtype I-E/Ecoli subfamily. FUNCTION: CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA).; FUNCTION: A component of Cascade, which participates in CRISPR interference, the third stage of CRISPR immunity. Cascade binds both crRNA and in a sequence-specific manner negatively supercoiled dsDNA target. This leads to the formation of an R-loop in which the crRNA binds the target DNA, displacing the noncomplementary strand. Cas3 is recruited to Cascade, nicks target DNA and then unwinds and cleaves the target, leading to DNA degradation and invader neutralization. CasCDE alone is also able to form R-loops. KEYWORDS: 3D-structure;Antiviral defense;Reference proteome " Q46899,"PROTEIN NAMES: CRISPR system Cascade subunit CasC ORGANISM: Escherichia coli (strain K12) FUNCTION: CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA).; FUNCTION: A component of Cascade, which participates in CRISPR interference, the third stage of CRISPR immunity. Cascade binds both crRNA and in a sequence-specific manner negatively supercoiled dsDNA target. This leads to the formation of an R-loop in which the crRNA binds the target DNA, displacing the noncomplementary strand. Cas3 is recruited to Cascade, nicks target DNA and then unwinds and cleaves the target, leading to DNA degradation and invader neutralization. CasCDE alone is also able to form R-loops. KEYWORDS: 3D-structure;Antiviral defense;Reference proteome;RNA-binding " Q46901,"PROTEIN NAMES: CRISPR system Cascade subunit CasA (CRISPR type I-E/Ecoli-associated protein CasA/Cse1) (CRISPR-associated protein CasA/Cse1) PROTEIN FAMILY: CRISPR associated protein CasA/Cse1 family, Type I-E/Ecoli subfamily ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the CRISPR associated protein CasA/Cse1 family. Type I-E/Ecoli subfamily. FUNCTION: CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA).; FUNCTION: A component of Cascade, which participates in CRISPR interference, the third stage of CRISPR immunity. Cascade binds both crRNA and in a sequence-specific manner negatively supercoiled dsDNA target. This leads to the formation of an R-loop in which the crRNA binds the target DNA, displacing the noncomplementary strand. Cas3 is recruited to Cascade, probably via interactions with CasA, nicks target DNA and then unwinds and cleaves the target, leading to DNA degradation and invader neutralization. CasA is not required for formation of Cascade, but probably enhances binding to and subsequent recognition of both target dsDNA and ssDNA. KEYWORDS: 3D-structure;Antiviral defense;DNA-binding;Reference proteome;RNA-binding " Q46927,"PROTEIN NAMES: tRNA threonylcarbamoyladenosine dehydratase (t(6)A37 dehydratase) PROTEIN FAMILY: HesA/MoeB/ThiF family ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the HesA/MoeB/ThiF family. FUNCTION: Catalyzes the ATP-dependent dehydration of threonylcarbamoyladenosine at position 37 (t(6)A37) to form cyclic t(6)A37 (ct(6)A37) in tRNAs that read codons beginning with adenine. TcdA is also part of a sulfur transfer pathway; is able to accept sulfur from CsdA directly in vitro, but CsdE might act as the sulfur donor in vivo. KEYWORDS: 3D-structure;ATP-binding;Ligase;Membrane;Nucleotide-binding;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Single-pass membrane protein MISCELLANEOUS: ct(6)A is involved in promoting decoding efficiency. It is an unstable modification that can be easily hydrolyzed and converted to t(6)A during nucleoside preparation by conventional methods. This explains why it was described as t(6)A37 in earlier studies." Q47474,PROTEIN NAMES: Pectinesterase B (PE B) (Pectin methylesterase B) PROTEIN FAMILY: Pectinesterase family ORGANISM: Dickeya dadantii (strain 3937) (Erwinia chrysanthemi (strain 3937)) SIMILARITY: Belongs to the pectinesterase family. FUNCTION: Probably involved in the degradation of methylated oligogalacturonides present in the periplasm. More active on methylated oligogalacturides than on pectin. PATHWAY: Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5. KEYWORDS: Aspartyl esterase;Cell outer membrane;Hydrolase;Lipoprotein;Membrane;Palmitate;Reference proteome;Signal SUBCELLULAR LOCATION: Cell outer membrane ; Lipid-anchor Note=Probably active in the periplasm. Q47914,"PROTEIN NAMES: Tetrachlorobenzoquinone reductase (TCBQ reductase) PROTEIN FAMILY: PDR/VanB family ORGANISM: Sphingobium chlorophenolicum SIMILARITY: Belongs to the PDR/VanB family. FUNCTION: Involved in the degradation of the xenobiocide pentachlorophenol (PCP). Catalyzes the reduction of tetrachlorobenzoquinone (TCBQ) to yield tetrachlorohydroquinone (TCHQ). Also able to reduce 2,6-dichloroindophenol (DCIP). PATHWAY: Xenobiotic degradation; pentachlorophenol degradation. KEYWORDS: 2Fe-2S;Aromatic hydrocarbons catabolism;Electron transport;Flavoprotein;FMN;Iron;Iron-sulfur;Metal-binding;NAD;Oxidoreductase;Transport " Q47KB1,"PROTEIN NAMES: Dye-decolorizing peroxidase Tfu_3078 (DyP) (Peroxidase Tfu_3078) PROTEIN FAMILY: DyP-type peroxidase family ORGANISM: Thermobifida fusca (strain YX) SIMILARITY: Belongs to the DyP-type peroxidase family. FUNCTION: Peroxidase that is able to convert a large number of compounds, but its physiological substrate is not known. Shows high reactivity towards anthraquinone dyes (e.g. Reactive Blue 19) and a modest activity towards standard peroxidase substrates (such as guaiacol and 2,6-dimethoxyphenol) and azo dyes (e.g. Reactive Blue 5). Is also able to oxidize aromatic sulfides enantioselectively, resulting in the corresponding (R)-sulfoxides, but with a poor efficiency. Does not display catalase activity. KEYWORDS: 3D-structure;Heme;Iron;Metal-binding;Oxidoreductase;Peroxidase;Secreted;Signal SUBCELLULAR LOCATION: Secreted " Q494U1,"PROTEIN NAMES: Pleckstrin homology domain-containing family N member 1 (PH domain-containing family N member 1) (Cardiolipin and phosphatidic acid-binding protein) ORGANISM: Homo sapiens (Human) FUNCTION: Controls the stability of the leptin mRNA harboring an AU-rich element (ARE) in its 3' UTR, in cooperation with the RNA stabilizer ELAVL1. Decreases the stability of the leptin mRNA by antagonizing the function of ELAVL1 by inducing its atypical recruitment from the nucleus to the cytosol (By similarity). Binds to cardiolipin (CL), phosphatidic acid (PA), phosphatidylinositol 4-phosphate (PtdIns(4)P) and phosphatidylserine (PS). Promotes apoptosis by enhancing BAX-BAK hetero-oligomerization via interaction with BID in colon cancer cells (By similarity). KEYWORDS: Alternative splicing;Cell membrane;Lipoprotein;Membrane;Mitochondrion;Myristate;Phosphoprotein;Reference proteome;Repeat SUBCELLULAR LOCATION: Cell membrane ; Lipid-anchor Mitochondrion Mitochondrion membrane Note=Interaction with C1QBP and phosphorylation is essential for its mitochondrial localization. Localizes on the microtubule in the form of small granules. " Q495C1,"PROTEIN NAMES: Probable E3 SUMO-protein ligase RNF212 (Probable E3 SUMO-protein transferase RNF212) (RING finger protein 212) ORGANISM: Homo sapiens (Human) FUNCTION: SUMO E3 ligase that acts as a regulator of crossing-over during meiosis: required to couple chromosome synapsis to the formation of crossover-specific recombination complexes. Localizes to recombination sites and stabilizes meiosis-specific recombination factors, such as MutS-gamma complex proteins (MSH4 and MSH5) and TEX11. May mediate sumoylation of target proteins MSH4 and/or MSH5, leading to enhance their binding to recombination sites. Acts as a limiting factor for crossover designation and/or reinforcement and plays an antagonist role with CCNB1IP1/HEI10 in the regulation of meiotic recombination (By similarity). PATHWAY: Protein modification; protein sumoylation. KEYWORDS: Alternative splicing;Chromosome;Coiled coil;Meiosis;Metal-binding;Nucleus;Reference proteome;Transferase;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Chromosome Note=Associates to the synaptonemal complex. Localizes to a minority of double-strand breaks (DSBs) sites. Marks crossover sites during midpachynema (By similarity). " Q495M9,"PROTEIN NAMES: pre-mRNA splicing regulator USH1G (Scaffold protein containing ankyrin repeats and SAM domain) (Usher syndrome type-1G protein) ORGANISM: Homo sapiens (Human) FUNCTION: Plays a role in pre-mRNA splicing by regulating the release and transfer of U4/U6.U5 tri-small nuclear ribonucleoprotein (tri-snRNP) complexes from their assembly site in Cajal bodies to nuclear speckles, thereby contributing to the assembly of the pre-catalytic spliceosome on target pre-mRNAs. May also participate in recycling of snRNPs back to Cajal bodies during splicing. Plays a role in regulating MAGI2-mediated endocytosis. Anchoring/scaffolding protein that is a part of the functional network formed by USH1C, USH1G, CDH23 and MYO7A that mediates mechanotransduction in cochlear hair cells. Required for normal development and maintenance of cochlear hair cell bundles. Required for normal hearing. KEYWORDS: 3D-structure;ANK repeat;Cell membrane;Cell projection;Cytoplasm;Cytoskeleton;Deafness;Disease variant;Hearing;Membrane;Nucleus;Phosphoprotein;Reference proteome;Repeat;Retinitis pigmentosa;Usher syndrome SUBCELLULAR LOCATION: Cytoplasm, cytosol. Cytoplasm, cytoskeleton. Cell membrane ; Peripheral membrane protein Cell projection, cilium Nucleus speckle Nucleus, Cajal body Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Photoreceptor inner segment Note=Detected at the tip of cochlear hair cell stereocilia. Recruited to the cell membrane via interaction with CDH23 or PCDH15 (By similarity). In photoreceptor cilia, detected predominantly at the cilium base (By similarity). Expressed in the pericentriolar region of the centrosome (By similarity). " Q498M5,"PROTEIN NAMES: E3 ubiquitin-protein ligase SH3RF2 (POSH-eliminating RING protein) (Protein phosphatase 1 regulatory subunit 39) (RING finger protein 158) (RING-type E3 ubiquitin transferase SH3RF2) (SH3 domain-containing RING finger protein 2) PROTEIN FAMILY: SH3RF family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the SH3RF family. FUNCTION: Has E3 ubiquitin-protein ligase activity. Acts as an anti-apoptotic regulator of the JNK pathway by ubiquitinating and promoting the degradation of SH3RF1, a scaffold protein that is required for pro-apoptotic JNK activation. Facilitates TNF-alpha-mediated recruitment of adapter proteins TRADD and RIPK1 to TNFRSF1A and regulates PAK4 protein stability via inhibition of its ubiquitin-mediated proteasomal degradation. Inhibits PPP1CA phosphatase activity (By similarity). PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Metal-binding;Nucleus;Phosphoprotein;Protein phosphatase inhibitor;Reference proteome;Repeat;SH3 domain;Transferase;Ubl conjugation;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus " Q498R1,"PROTEIN NAMES: Methionine synthase reductase (MSR) (Aquacobalamin reductase) (AqCbl reductase) ORGANISM: Rattus norvegicus (Rat) FUNCTION: Key enzyme in methionine and folate homeostasis responsible for the reactivation of methionine synthase (MTR/MS) activity by catalyzing the reductive methylation of MTR-bound cob(II)alamin. Cobalamin (vitamin B12) forms a complex with MTR to serve as an intermediary in methyl transfer reactions that cycles between MTR-bound methylcob(III)alamin and MTR bound-cob(I)alamin forms, and occasional oxidative escape of the cob(I)alamin intermediate during the catalytic cycle leads to the inactive cob(II)alamin species. The processing of cobalamin in the cytosol occurs in a multiprotein complex composed of at least MMACHC, MMADHC, MTRR and MTR which may contribute to shuttle safely and efficiently cobalamin towards MTR in order to produce methionine (By similarity). Also necessary for the utilization of methyl groups from the folate cycle, thereby affecting transgenerational epigenetic inheritance (By similarity). Also acts as a molecular chaperone for methionine synthase by stabilizing apoMTR and incorporating methylcob(III)alamin into apoMTR to form the holoenzyme. Also serves as an aquacob(III)alamin reductase by reducing aquacob(III)alamin to cob(II)alamin; this reduction leads to stimulation of the conversion of apoMTR and aquacob(III)alamin to MTR holoenzyme (By similarity). KEYWORDS: Alternative splicing;Amino-acid biosynthesis;Cytoplasm;FAD;Flavoprotein;FMN;Methionine biosynthesis;NADP;Oxidoreductase;Phosphoprotein;Reference proteome;S-adenosyl-L-methionine SUBCELLULAR LOCATION: Cytoplasm MISCELLANEOUS: It is debated whether the reduction of free aquacob(II)alamin occurs spontaneously or is enzyme catalyzed." Q49AM1,"PROTEIN NAMES: Transcription termination factor 2, mitochondrial (Mitochondrial transcription termination factor 2) (mTERF2) (Mitochondrial transcription termination factor-like protein) (mTERF-like) (mTERFL) (mTERF domain-containing protein 3, mitochondrial) PROTEIN FAMILY: MTERF family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the mTERF family. FUNCTION: Binds mitochondrial DNA and plays a role in the regulation of transcription of mitochondrial mRNA and rRNA species. KEYWORDS: Direct protein sequencing;Mitochondrion;Mitochondrion nucleoid;Reference proteome;Transcription;Transcription regulation;Transit peptide SUBCELLULAR LOCATION: Mitochondrion Mitochondrion matrix, mitochondrion nucleoid " Q49BZ4,"PROTEIN NAMES: C-type lectin domain family 7 member A (Dendritic cell-associated C-type lectin 1) (DC-associated C-type lectin 1) (Dectin-1) (CD antigen CD369) ORGANISM: Bos taurus (Bovine) FUNCTION: Lectin that functions as a pattern recognizing receptor (PRR) specific for beta-1,3-linked and beta-1,6-linked glucans, which constitute cell wall constituents from pathogenic bacteria and fungi. Necessary for the TLR2-mediated inflammatory response and activation of NF-kappa-B: upon beta-glucan binding, recruits SYK via its ITAM motif and promotes a signaling cascade that activates some CARD domain-BCL10-MALT1 (CBM) signalosomes, leading to the activation of NF-kappa-B and MAP kinase p38 (MAPK11, MAPK12, MAPK13 and/or MAPK14) pathways which stimulate expression of genes encoding pro-inflammatory cytokines and chemokines. Enhances cytokine production in macrophages and dendritic cells. Mediates production of reactive oxygen species in the cell. Mediates phagocytosis of C.albicans conidia. Binds T-cells in a way that does not involve their surface glycans and plays a role in T-cell activation. Stimulates T-cell proliferation. Induces phosphorylation of SCIMP after binding beta-glucans. KEYWORDS: Alternative splicing;Cell membrane;Disulfide bond;Glycoprotein;Immunity;Inflammatory response;Innate immunity;Lectin;Membrane;Metal-binding;Phosphoprotein;Reference proteome;Signal-anchor;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type II membrane protein " Q49GP3,"PROTEIN NAMES: Phosphatidylinositol 4-kinase beta (PI4K-beta) (PI4Kbeta) (PtdIns 4-kinase beta) PROTEIN FAMILY: PI3/PI4-kinase family, Type III PI4K subfamily ORGANISM: Danio rerio (Zebrafish) (Brachydanio rerio) SIMILARITY: Belongs to the PI3/PI4-kinase family. Type III PI4K subfamily. FUNCTION: Phosphorylates phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate (PIP). May play an important role the in inner ear development. KEYWORDS: ATP-binding;Endoplasmic reticulum;Kinase;Lipid metabolism;Membrane;Mitochondrion;Mitochondrion outer membrane;Nucleotide-binding;Reference proteome;Transferase SUBCELLULAR LOCATION: Endomembrane system Mitochondrion outer membrane ; Peripheral membrane protein Rough endoplasmic reticulum membrane ; Peripheral membrane protein " Q49MI3,"PROTEIN NAMES: Ceramide kinase-like protein ORGANISM: Homo sapiens (Human) FUNCTION: Has no detectable ceramide-kinase activity. Overexpression of CERKL protects cells from apoptosis in oxidative stress conditions. KEYWORDS: Alternative splicing;Cytoplasm;Disease variant;Endoplasmic reticulum;Golgi apparatus;Nucleus;Phosphoprotein;Reference proteome;Retinitis pigmentosa SUBCELLULAR LOCATION: Cytoplasm. Nucleus, nucleolus. Note=Enriched in nucleoli. May shuttle between nucleus and cytoplasm. Isoform 5 is not enriched in the nucleoli.; SUBCELLULAR LOCATION: [Isoform 2]: Cytoplasm. Nucleus, nucleolus. Golgi apparatus, trans-Golgi network. Endoplasmic reticulum. MISCELLANEOUS: [Isoform 5]: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.; MISCELLANEOUS: [Isoform 7]: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.; MISCELLANEOUS: [Isoform 8]: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay." Q49QW1,"PROTEIN NAMES: Phenoloxidase-activating enzyme 1 (Prophenoloxidase-activating enzyme 1) (Serine protease PPAE1) (Slppae1) PROTEIN FAMILY: Peptidase S1 family, CLIP subfamily ORGANISM: Spodoptera litura (Asian cotton leafworm) SIMILARITY: Belongs to the peptidase S1 family. CLIP subfamily. FUNCTION: Serine protease which, by cleaving and activating prophenoloxidase (PPO1) after immune challenge, plays an essential role in the melanization immune response to wounding. KEYWORDS: Calcium;Disulfide bond;Hydrolase;Metal-binding;Protease;Reference proteome;Secreted;Serine protease;Signal;Zymogen SUBCELLULAR LOCATION: Secreted Note=Secreted in the hemolymph. " Q49SH1,"PROTEIN NAMES: Sodium/potassium/calcium exchanger 5 (Na(+)/K(+)/Ca(2+)-exchange protein 5) (Protein golden) (Solute carrier family 24 member 5) PROTEIN FAMILY: Ca(2+):cation antiporter (CaCA) family, SLC24A subfamily ORGANISM: Danio rerio (Zebrafish) (Brachydanio rerio) SIMILARITY: Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19) family. SLC24A subfamily. FUNCTION: Calcium, potassium:sodium antiporter that transports 1 Ca(2+) and 1 K(+) to the melanosome in exchange for 4 cytoplasmic Na(+) (By similarity). Involved in pigmentation, possibly by participating in ion transport in melanosomes. Predominant sodium-calcium exchanger in melanocytes. KEYWORDS: Antiport;Calcium;Calcium transport;Golgi apparatus;Ion transport;Membrane;Potassium;Potassium transport;Reference proteome;Sensory transduction;Signal;Sodium;Sodium transport;Symport;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Golgi apparatus, trans-Golgi network membrane ; Multi-pass membrane protein Melanosome Note=Enriched in late-stage melanosomes. " Q4DA80,"PROTEIN NAMES: Proline racemase A (TcPA45-A) (TcPRACA) (rTcPA45) PROTEIN FAMILY: Proline racemase family ORGANISM: Trypanosoma cruzi (strain CL Brener) SIMILARITY: Belongs to the proline racemase family. FUNCTION: Catalyzes the interconversion of L- and D-proline. Secreted isoform 1 contributes to parasite immune evasion by acting as a B-cell mitogen. Probably involved in parasite differentiation and infectivity. KEYWORDS: 3D-structure;Alternative splicing;Cytoplasm;Developmental protein;Glycoprotein;Isomerase;Membrane;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: [Isoform 1]: Secreted Membrane Note=Membrane-bound and secreted upon differentiation of the parasite into non-dividing infective forms, suggesting that isoform 1 appears upon differentiation.; SUBCELLULAR LOCATION: [Isoform 2]: Cytoplasm Note=Cytoplasmic in replicative non-infective forms. " Q4FZD7,"PROTEIN NAMES: Inactive serine/threonine-protein kinase PLK5 (Polo-like kinase 5) (PLK-5) PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family, CDC5/Polo subfamily ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily. FUNCTION: Inactive serine/threonine-protein kinase that plays a role in cell cycle progression and neuronal differentiation. KEYWORDS: ATP-binding;Cell cycle;Cell division;Cytoplasm;Differentiation;Magnesium;Metal-binding;Nucleotide-binding;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus, nucleolus Cytoplasm " Q4G0U5,"PROTEIN NAMES: Cilia- and flagella-associated protein 221 (Primary ciliary dyskinesia protein 1) PROTEIN FAMILY: PCDP1 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the PCDP1 family. FUNCTION: May play a role in cilium morphogenesis. KEYWORDS: Alternative splicing;Calmodulin-binding;Cell projection;Cilium;Cilium biogenesis/degradation;Cytoplasm;Cytoskeleton;Reference proteome SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, cilium axoneme Cytoplasm Cytoplasm, cytoskeleton Note=Localizes to the manchette in elongating spermatids in a SPAG17-dependent manner. " Q4G163,PROTEIN NAMES: F-box only protein 43 (Endogenous meiotic inhibitor 2) ORGANISM: Homo sapiens (Human) FUNCTION: Required to establish and maintain the arrest of oocytes at the second meiotic metaphase until fertilization. Acts by inhibiting the anaphase-promoting complex/cyclosome (APC/C) ubiquitin ligase. Probably recognizes and binds to some phosphorylated proteins and promotes their ubiquitination and degradation. Plays a vital role in modulating the ubiquitilation of CCNB1 and CDK1 during gametogenesis. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Disease variant;Meiosis;Metal-binding;Phosphoprotein;Reference proteome;Ubl conjugation;Ubl conjugation pathway;Zinc;Zinc-finger Q4JA33,"PROTEIN NAMES: Digeranylgeranylglycerophospholipid reductase (DGGGPL reductase) (2,3-bis-O-geranylgeranylglyceryl phosphate reductase) (Geranylgeranyl diphosphate reductase) (GGPP reductase) (Geranylgeranyl reductase) (GGR) PROTEIN FAMILY: Geranylgeranyl reductase family ORGANISM: Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770) SIMILARITY: Belongs to the geranylgeranyl reductase family. FUNCTION: Is involved in the reduction of 2,3-digeranylgeranylglycerophospholipids (unsaturated archaeols) into 2,3-diphytanylglycerophospholipids (saturated archaeols) in the biosynthesis of archaeal membrane lipids. Catalyzes the formation of archaetidic acid (2,3-di-O-phytanyl-sn-glyceryl phosphate) from 2,3-di-O-geranylgeranylglyceryl phosphate (DGGGP) via the hydrogenation of each double bond of the isoprenoid chains. Is not active with NADPH or NADH as an electron donor; the physiological reducing agent is unknown. Is also active on the more upstream precursors of membrane lipid biosynthesis, catalyzing the complete reduction of 3-O-geranylgeranylglyceryl phosphate (GGGP) to 3-O-phytanylglyceryl phosphate, and the partial reduction of geranylgeranyl diphosphate (GGPP) to phytyl diphosphate, thus reducing three of four GGPP double bonds and preserving the allylic double bond (at position 2). This reaction product is a reactive prenyl donor, which can be used as a substrate by archaeal prenyltransferases such as GGGP synthases. PATHWAY: Membrane lipid metabolism; glycerophospholipid metabolism. KEYWORDS: 3D-structure;Disulfide bond;FAD;Flavoprotein;Lipid biosynthesis;Lipid metabolism;Oxidoreductase;Phospholipid biosynthesis;Phospholipid metabolism;Reference proteome " Q4JB16,"PROTEIN NAMES: tRNA (cytidine-2'-O-)-methyltransferase TrmJ (tRNA (cytidine(32)-2'-O)-methyltransferase) (tRNA Cm32 methyltransferase) PROTEIN FAMILY: Class IV-like SAM-binding methyltransferase superfamily, RNA methyltransferase TrmH family ORGANISM: Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770) SIMILARITY: Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family. FUNCTION: Catalyzes the formation of 2'O-methylated cytidine (Cm32) at position 32 in tRNA. Is specific for cytidine. KEYWORDS: 3D-structure;Cytoplasm;Methyltransferase;Reference proteome;S-adenosyl-L-methionine;Transferase;tRNA processing SUBCELLULAR LOCATION: Cytoplasm " Q4K418,"PROTEIN NAMES: Phloroglucinol synthase (Type III polyketide synthase PhlD) PROTEIN FAMILY: Thiolase-like superfamily, Chalcone/stilbene synthases family ORGANISM: Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5) SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene synthases family. FUNCTION: Type III polyketide synthase that catalyzes the synthesis of phloroglucinol from three molecules of malonyl-CoA. In addition to its ability to produce phloroglucinol from malonyl-CoA, it exhibits broad substrate specificity, accepting C4-C12 aliphatic acyl-CoAs and phenylacetyl-CoA as the starters to form C6-polyoxoalkylated alpha-pyrones from sequential condensation with malonyl-CoA. PATHWAY: Antibiotic biosynthesis. KEYWORDS: Acyltransferase;Antibiotic biosynthesis;Transferase " Q4KLN5,"PROTEIN NAMES: Phospholipase A and acyltransferase 5 (Ca(2+)-independent N-acyltransferase) (iNAT) (H-rev107-like protein 5) (HRAS-like suppressor 5) (HRSL5) (Rat LRAT-like protein-1) (RLP-1) PROTEIN FAMILY: H-rev107 family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the H-rev107 family. FUNCTION: Exhibits both phospholipase A1/2 and acyltransferase activities (By similarity). Shows phospholipase A1 (PLA1) and A2 (PLA2) activity, catalyzing the calcium-independent release of fatty acids from the sn-1 or sn-2 position of glycerophospholipids (By similarity). Shows N-acyltransferase activity, catalyzing the calcium-independent transfer of a fatty acyl group at the sn-1 position of phosphatidylcholine (PC) and other glycerophospholipids to the primary amine of phosphatidylethanolamine (PE), forming N-acylphosphatidylethanolamine (NAPE), which serves as precursor for N-acylethanolamines (NAEs). KEYWORDS: Acyltransferase;Cytoplasm;Hydrolase;Lipid metabolism;Reference proteome;Transferase SUBCELLULAR LOCATION: Cytoplasm, cytosol " Q4KM34,"PROTEIN NAMES: Cyclin-dependent kinase 20 (Cell cycle-related kinase) (Cell division protein kinase 20) PROTEIN FAMILY: Protein kinase superfamily, CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. FUNCTION: Required for high-level Shh responses in the developing neural tube. Together with TBC1D32, controls the structure of the primary cilium by coordinating assembly of the ciliary membrane and axoneme, allowing GLI2 to be properly activated in response to SHH signaling. Involved in cell growth. Activates CDK2, a kinase involved in the control of the cell cycle, by phosphorylating residue 'Thr-160' (By similarity). KEYWORDS: ATP-binding;Cell cycle;Cell division;Cell projection;Cilium;Cytoplasm;Developmental protein;Kinase;Nucleotide-binding;Nucleus;Reference proteome;Serine/threonine-protein kinase;Transferase SUBCELLULAR LOCATION: Nucleus Cytoplasm Cell projection, cilium " Q4KMD7,"PROTEIN NAMES: Speckle targeted PIP5K1A-regulated poly(A) polymerase (Star-PAP) (RNA-binding motif protein 21) (RNA-binding protein 21) (U6 snRNA-specific terminal uridylyltransferase 1) (U6-TUTase) PROTEIN FAMILY: DNA polymerase type-B-like family ORGANISM: Danio rerio (Zebrafish) (Brachydanio rerio) SIMILARITY: Belongs to the DNA polymerase type-B-like family. FUNCTION: Poly(A) polymerase that creates the 3'-poly(A) tail of specific pre-mRNAs. In addition to polyadenylation, it is also required for the 3'-end cleavage of pre-mRNAs: binds to the 3'UTR of targeted pre-mRNAs and promotes the recruitment and assembly of the CPSF complex on the 3'UTR of pre-mRNAs. In addition to adenylyltransferase activity, also has uridylyltransferase activity. However, the ATP ratio is higher than UTP in cells, suggesting that it functions primarily as a poly(A) polymerase. KEYWORDS: ATP-binding;Magnesium;Manganese;Metal-binding;mRNA processing;Nucleotide-binding;Nucleotidyltransferase;Nucleus;Reference proteome;RNA-binding;Transferase;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus, nucleolus Nucleus speckle " Q4KSH9,"PROTEIN NAMES: Alpha-barbatene synthase (AtBS) (Beta-chamigrene synthase) (Terpeneoid synthase 11) (AtTPS11) (Thujopsene synthase) PROTEIN FAMILY: Terpene synthase family, Tpsa subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the terpene synthase family. Tpsa subfamily. FUNCTION: Involved in the biosynthesis of over 15 sesquiterpenes (C15). The major products are (+)-alpha-barbatene (27.3%), (+)-thujopsene (17.8%) and (+)-beta-chamigrene (9.9%). Can use farnesyl diphosphate or geranyl diphosphate as substrates, but not geranylgeranyl diphosphate. PATHWAY: Secondary metabolite biosynthesis; terpenoid biosynthesis. KEYWORDS: Cytoplasm;Lyase;Magnesium;Manganese;Metal-binding;Reference proteome SUBCELLULAR LOCATION: Cytoplasm " Q4LBB9,"PROTEIN NAMES: Octopamine receptor beta-2R (DmOct-beta-12) (DmOct-beta-2R) PROTEIN FAMILY: G-protein coupled receptor 1 family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the G-protein coupled receptor 1 family. FUNCTION: Autoreceptor for octopamine (OA), which is a neurotransmitter, neurohormone, and neuromodulator in invertebrates. Essential for ovulation and fertilization. During ovulation it mediates the OA-induced relaxation of the oviduct visceral muscles, by increasing cAMP levels and activating effectors such as calmodulin-dependent kinase II (CaMKII) and cAMP-dependent protein kinase A (PKA) pathways. Positively regulates synaptic growth; an action that is antagonized by Octbeta1R. KEYWORDS: Cell membrane;G-protein coupled receptor;Glycoprotein;Membrane;Receptor;Reference proteome;Transducer;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein " Q4PIR3,PROTEIN NAMES: E3 SUMO-protein ligase nse2 (E3 SUMO-protein transferase nse2) (Non-structural maintenance of chromosomes element 2) (Non-SMC element 2) PROTEIN FAMILY: NSE2 family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the NSE2 family. FUNCTION: Acts as an E3 ligase mediating SUMO/Smt3 attachment to other proteins. Acts in a DNA repair pathway for removal of UV-induced DNA damage that is distinct from classical nucleotide excision repair and in repair of ionizing radiation damage. Functions in homologous recombination repair of DNA double strand breaks and in recovery of stalled replication forks. Plays a critical role in meiosis. PATHWAY: Protein modification; protein sumoylation. KEYWORDS: DNA damage;DNA recombination;DNA repair;Meiosis;Metal-binding;Nucleus;Reference proteome;Transferase;Ubl conjugation;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Q4PNI0,"PROTEIN NAMES: Beta-carotene 15,15'-dioxygenase PROTEIN FAMILY: Brp/Blh beta-carotene diooxygenase family ORGANISM: Uncultured marine bacterium 66A03 SIMILARITY: Belongs to the Brp/Blh beta-carotene diooxygenase family. FUNCTION: Catalyzes the cleavage of beta-carotene at its central double bond (15,15') to yield two molecules of all-trans-retinal. Exhibits the highest activity for beta-carotene, followed by beta-cryptoxanthin, beta-apo-4'-carotenal, alpha-carotene, and gamma-carotene in decreasing order, but has no activity on beta-apo-8'-carotenal, beta-apo-12'-carotenal, lutein, zeaxanthin, or lycopene, suggesting that the presence of one unsubstituted beta-ionone ring in a substrate with a molecular weight greater than C35 seems to be essential for enzyme activity. KEYWORDS: Cell membrane;Dioxygenase;Iron;Membrane;Metal-binding;Oxidoreductase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein " Q4Q0M0,"PROTEIN NAMES: Selenide, water dikinase (LmselD) (Selenophosphate synthetase 2) PROTEIN FAMILY: Selenophosphate synthase 1 family, Class I subfamily ORGANISM: Leishmania major SIMILARITY: Belongs to the selenophosphate synthase 1 family. Class I subfamily. FUNCTION: Synthesizes selenophosphate from selenide and ATP. KEYWORDS: 3D-structure;ATP-binding;Kinase;Magnesium;Metal-binding;Nucleotide-binding;Reference proteome;Selenium;Transferase " Q4QIU7,"PROTEIN NAMES: Protoporphyrinogen IX dehydrogenase [quinone] (Protoporphyrinogen IX dehydrogenase [menaquinone]) (Protoporphyrinogen IX dehydrogenase [ubiquinone]) (Protoporphyrinogen oxidase) (PPO) PROTEIN FAMILY: HemG family ORGANISM: Leishmania major SIMILARITY: Belongs to the HemG family. FUNCTION: In E.coli extracts under anerobic conditions catalyzes the 6-electron oxidation of protoporphyrinogen IX to form protoporphyrin IX, transferring electrons to fumarate reductase, presumably via menaquinone. In vitro under aerobic conditions forms protoporphyrin IX using ubiquinone as an electron acceptor. Complements an E.coli hemG deletion, allowing normal growth in vivo. PATHWAY: Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; protoporphyrin-IX from protoporphyrinogen-IX: step 1/1. KEYWORDS: Flavoprotein;FMN;Membrane;Nucleotide-binding;Oxidoreductase;Porphyrin biosynthesis;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Single-pass membrane protein MISCELLANEOUS: Leishmania is not able to completely synthesize heme; it is thought that the amastigote form localized in macrophages utilizes coproporphyrinogen from the host to produce heme via the successive action of HemF, HemG and HemH." Q4QTJ1,"PROTEIN NAMES: S-norcoclaurine synthase 2 (PsNCS2) PROTEIN FAMILY: BetVI family ORGANISM: Papaver somniferum (Opium poppy) SIMILARITY: Belongs to the BetVI family. FUNCTION: Involved in the biosynthesis of (S)-coclaurine, the common precursor of all benzylisoquinoline alkaloids such as morphine, sanguinarine, codeine or papaverine. Condenses dopamine and 4-hydroxyphenylacetaldehyde. PATHWAY: Alkaloid biosynthesis; (S)-reticuline biosynthesis. KEYWORDS: Alkaloid metabolism;Endoplasmic reticulum;Lyase;Membrane;Signal;Transmembrane;Transmembrane helix;Vacuole SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Single-pass membrane protein Vacuole membrane ; Single-pass membrane protein Note=Initially localized to the endoplasmic reticulum but subsequently sorted to the vacuole. MISCELLANEOUS: The 40 amino acid N-terminal extension should included a signal peptide targeting the mature protein to the endoplasmic reticulum." Q4R1C4,"PROTEIN NAMES: Exo-beta-D-glucosaminidase PROTEIN FAMILY: Glycosyl hydrolase 2 family ORGANISM: Hypocrea jecorina (Trichoderma reesei) SIMILARITY: Belongs to the glycosyl hydrolase 2 family. FUNCTION: Hydrolyzes chitosan and chitooligosaccharides with retention of anomeric configuration. Has no activity against beta-D-galactoside, beta-D-glucuronide, beta-D-mannoside, chitin, glycol chitosan, cellulose, N,N'-diacetylchitibiose and pNP-GlcNAc. KEYWORDS: Carbohydrate metabolism;Chitin degradation;Direct protein sequencing;Glycoprotein;Glycosidase;Hydrolase;Polysaccharide degradation;Secreted;Signal SUBCELLULAR LOCATION: Secreted, extracellular space " Q4R7L8,"PROTEIN NAMES: NAD-capped RNA hydrolase NUDT12 (DeNADding enzyme NUDT12) (NADH pyrophosphatase NUDT12) (Nucleoside diphosphate-linked moiety X motif 12) (Nudix motif 12) PROTEIN FAMILY: Nudix hydrolase family, NudC subfamily ORGANISM: Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) SIMILARITY: Belongs to the Nudix hydrolase family. NudC subfamily. FUNCTION: mRNA decapping enzyme that specifically removes the nicotinamide adenine dinucleotide (NAD) cap from a subset of mRNAs by hydrolyzing the diphosphate linkage to produce nicotinamide mononucleotide (NMN) and 5' monophosphate mRNA. The NAD-cap is present at the 5'-end of some RNAs; in contrast to the canonical N7 methylguanosine (m7G) cap, the NAD cap promotes mRNA decay. Preferentially acts on NAD-capped transcripts in response to nutrient stress (By similarity). Also acts on free nicotinamide adenine dinucleotide molecules: hydrolyzes NAD(H) into NMN(H) and AMP, and NADPH into NMNH and 2',5'-ADP. May act to regulate the concentration of peroxisomal nicotinamide nucleotide cofactors required for oxidative metabolism in this organelle (By similarity). Regulates the levels of circadian clock components PER1, PER2, PER3 and CRY2 in the liver (By similarity). KEYWORDS: ANK repeat;Cytoplasm;Hydrolase;Magnesium;Metal-binding;NAD;NADP;Peroxisome;Reference proteome;Repeat;Zinc SUBCELLULAR LOCATION: Cytoplasm Peroxisome Cytoplasmic granule Note=Localizes to cytoplasmic granules in the presence of BLMH. " Q4TT88,"PROTEIN NAMES: Puromycin-sensitive aminopeptidase (PSA) (Cytosol alanyl aminopeptidase) (AAP-S) PROTEIN FAMILY: Peptidase M1 family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the peptidase M1 family. FUNCTION: Aminopeptidase. Required for the exit from meiosis, probably upstream of cyclin cyb-3. Involved in the establishment of the anterior-posterior polarity at the embryonic 1-cell stage by regulating the dynamics of sperm-donated centrosomes. Plays a role in oocyte maturation. Required for embryonic development. KEYWORDS: Alternative splicing;Aminopeptidase;Chromosome;Cytoplasm;Cytoskeleton;Differentiation;Hydrolase;Meiosis;Metal-binding;Metalloprotease;Oogenesis;Protease;Reference proteome;Zinc SUBCELLULAR LOCATION: Cytoplasm Cytoplasm, cell cortex Chromosome Cytoplasm, cytoskeleton, spindle pole Note=During meiosis I, localizes to the cell cortex. During meiosis II, localizes to the cytoplasm. During embryonic mitosis, localizes around mitotic chromosomes at metaphase and anaphase and near the spindle poles. In spermatids, excluded from the chromosomes. " Q4U331,"PROTEIN NAMES: Delta(1)-pyrroline-2-carboxylate/Delta(1)-piperideine-2-carboxylate reductase (Pyr2C/Pip2C reductase) (N-methyl-L-amino acid dehydrogenase) PROTEIN FAMILY: LDH2/MDH2 oxidoreductase family ORGANISM: Pseudomonas syringae pv. tomato SIMILARITY: Belongs to the LDH2/MDH2 oxidoreductase family. FUNCTION: Catalyzes the reduction of both Delta(1)-pyrroline-2-carboxylate (Pyr2C) and Delta(1)-piperideine-2-carboxylate (Pip2C) to L-proline and L-pipecolate, respectively, using NADPH as the electron donor. Can catalyze the reverse oxidation reactions, albeit at a much lower rate. Is also able to catalyze in vitro the NADPH-dependent formation of N-methylalanine from pyruvate and N-methylamine; can act on other alpha-keto acids and specifically uses methylamine and not ammonia for these reductive amination reactions. Can use NADH instead of NADPH, although with much less efficiency. KEYWORDS: 3D-structure;NADP;Nucleotide-binding;Oxidoreductase " Q4UWF4,"PROTEIN NAMES: Uridine 5'-monophosphate transferase (UMP transferase) (Type III effector XopAC/AvrAC) (Uridylyl transferase) PROTEIN FAMILY: Fic family ORGANISM: Xanthomonas campestris pv. campestris (strain 8004) SIMILARITY: In the C-terminal section; belongs to the fic family. FUNCTION: Functions both as a virulence and an avirulence gene in Arabidopsis. Causes disease on the Kashmir (Kas) ecotype, but not on Columbia (Col-0) ecotype. Acts by directly uridylylating the conserved phosphorylation sites in the activation loop of a number of host receptor-like cytoplasmic protein kinases (RLCK), including BIK1, RIPK, PBL1 and PBL2, preventing the activation of these kinases and subsequent signal transduction. In susceptible Arabidopsis plants, uridylylation of BIK1 inhibits the PAMP-triggered immunity (PTI) signaling cascade and thereby promotes bacterial virulence. It also inhibits RPM1-dependent effector-triggered immunity (ETI) in mesophyll tissues by targeting RIPK. In contrast, in the resistant ecotype Col-0, xopAC is a major avirulence gene. Uridylylation of PBL2 triggers the PBL2-RKS1 interaction and thus the assembly of the PBL2-RKS1-ZAR1 complex, which, in turn, activates effector-triggered immunity (ETI) against X.campestris. KEYWORDS: Host cell membrane;Host membrane;Leucine-rich repeat;Membrane;Nucleotidyltransferase;Repeat;Secreted;Transferase;Virulence SUBCELLULAR LOCATION: Secreted Host cell Host cell membrane Note=Secreted via the type III secretion system (T3SS) (Probable). Localization to the host plasma membrane requires the LRR domain. " Q4V3D2,"PROTEIN NAMES: Aspartic proteinase 36 PROTEIN FAMILY: Peptidase A1 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the peptidase A1 family. FUNCTION: Displays aspartic proteolytic activity. Together with A39, contributes to pollen and ovule development, including the apical cell wall constitution of the growing pollen tubes. KEYWORDS: Aspartyl protease;Cell membrane;Cytoplasm;Disulfide bond;Glycoprotein;GPI-anchor;Hydrolase;Lipoprotein;Membrane;Protease;Reference proteome;Signal SUBCELLULAR LOCATION: Cell membrane ; Lipid-anchor, GPI-anchor Cytoplasm, cytosol Note=Displays punctate cytosolic localization and colocalizes with the GPI-anchored protein COBL10 at the plasma membrane and in the cytosol. In pollen grains, mainly present at the plasma membrane and in reticular structures in the cytosol. In pollen tubes, exhibits a punctate localization in the cytosol and occurs weakly at the plasma membrane. " Q4V8K1,"PROTEIN NAMES: Metalloreductase STEAP4 (Six-transmembrane epithelial antigen of prostate 4) PROTEIN FAMILY: STEAP family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the STEAP family. FUNCTION: Integral membrane protein that functions as a NADPH-dependent ferric-chelate reductase, using NADPH from one side of the membrane to reduce a Fe(3+) chelate that is bound on the other side of the membrane. Mediates sequential transmembrane electron transfer from NADPH to FAD and onto heme, and finally to the Fe(3+) chelate. Can also reduce Cu(2+) to Cu(1+). Plays a role in systemic metabolic homeostasis, integrating inflammatory and metabolic responses (By similarity). Associated with obesity and insulin-resistance (By similarity). Involved in inflammatory arthritis, through the regulation of inflammatory cytokines (By similarity). Inhibits anchorage-independent cell proliferation (By similarity). KEYWORDS: 3D-structure;Cell membrane;Copper;Endosome;FAD;Flavoprotein;Golgi apparatus;Heme;Ion transport;Iron;Iron transport;Membrane;Metal-binding;Oxidoreductase;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Golgi apparatus membrane ; Multi-pass membrane protein Early endosome membrane ; Multi-pass membrane protein " Q4VC44,"PROTEIN NAMES: FLYWCH-type zinc finger-containing protein 1 ORGANISM: Homo sapiens (Human) FUNCTION: Transcription cofactor. Negatively regulates transcription activation by catenin beta-1 CTNNB1, perhaps acting by competing with TCF4 for CTNNB1 binding. May play a role in DNA-damage response signaling. Binds specifically to DNA sequences at peri-centromeric chromatin loci. KEYWORDS: 3D-structure;Alternative splicing;Centromere;Chromosome;DNA damage;DNA-binding;Isopeptide bond;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Repeat;Transcription;Transcription regulation;Ubl conjugation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Chromosome, centromere Note=Localized to peri-centromeric, H3K9me3-marked heterochromatin. " Q4VCM1,"PROTEIN NAMES: Phospholipid--sterol O-acyltransferase (Lecithin-cholesterol acyltransferase-like 2) PROTEIN FAMILY: AB hydrolase superfamily, Lipase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the AB hydrolase superfamily. Lipase family. FUNCTION: Involved in lipid catabolism. Essential for sterol esters biosynthesis in leaves and seeds, but not in flowers. Plays a role in controlling the free sterol content of leaves. Catalyzes the transacylation of acyl groups from phospholipids to a variety of different sterols. Prefers phosphatidylethanolamine over phosphatidylcholine as an acyl donor. Not active toward neutral lipids. Highly specific for position sn-2, which in plant lipids is essentially devoid of saturated acyl groups. Broad sterol specificity (cholesterol > campesterol > sitosterol > stigmasterol), but no activity with lupeol or beta-amyrin. KEYWORDS: Acyltransferase;Endoplasmic reticulum;Lipid metabolism;Membrane;Microsome;Reference proteome;Signal-anchor;Steroid metabolism;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Microsome membrane ; Single-pass type II membrane protein " Q4WD43,"PROTEIN NAMES: Amino acid oxidase fsqB (Fumipyrrole biosynthesis protein A) (Fumisoquins biosynthesis protein B) PROTEIN FAMILY: MSOX/MTOX family ORGANISM: Aspergillus fumigatus (strain ATCC MYA-4609 / CBS 101355 / FGSC A1100 / Af293) (Neosartorya fumigata) SIMILARITY: Belongs to the MSOX/MTOX family. FUNCTION: Amino acid oxidase; part of the gene cluster that mediates the biosynthesis of the isoquinoline alkaloids fumisoquin A, fumisoquin B and fumisoquin C; as well as small amounts of fumipyrrole as a shunt metabolite. The products of the cluster lead to a brown coloration and are important for growth and conidiation. The nonribosomal peptide synthetase-like protein fsqF, which lacks a canonical condensation domain, is required for addition of a serine-derived dehydroalanine moiety to activated tyrosine but is not essential for the subsequent steps leading to isoquinoline formation. A different enzyme, most likely the ATP-grasp enzyme fsqD, is responsible for activation of tyrosine (Probable). Three additional enzymes encoded by the fsq cluster, the N-methyltransferase fsqC, the phenol 2-monooxygenase fsqG and the FAD-dependent oxidase fsqB, catalyze the formation of the isoquinoline ring system in the fumisoquins. FsqB converts the fspF thiolation domain-bound (2S,4S,5S)-2-amino-6-(3,4-dihydroxyphenyl)-4-hydroxy-5-(methylamino)hexanoyl into isoquinoline. The cyclization most likely proceeds via a two-step mechanism, beginning with FAD-dependent oxidation of the methyl group to an iminium species followed by electrophilic attack on the deprotonated phenol (Probable).; FUNCTION: Is able to convert N-methyl-3,4-dihydroxy-DL-phenylalanine (N-methyl-DOPA) directly into cyclic isoquinoline, in vitro. The absence of the meta-hydroxyl group, as in L-N-methyl-tyrosine, leads to a 25-fold lower rate of reduction and the formation of the demethylated product L-tyrosine, instead of a cyclic product. Does not accept the D-stereoisomer of N-methyltyrosine, in contrast to N-methyl-DOPA, for which both stereoisomers are oxidized with similar rates. PATHWAY: Secondary metabolite biosynthesis. KEYWORDS: 3D-structure;FAD;Flavoprotein;Oxidoreductase;Reference proteome " Q4WGL5,"PROTEIN NAMES: Secreted beta-glucosidase sun1 PROTEIN FAMILY: SUN family ORGANISM: Aspergillus fumigatus (strain ATCC MYA-4609 / CBS 101355 / FGSC A1100 / Af293) (Neosartorya fumigata) SIMILARITY: Belongs to the SUN family. FUNCTION: Cell surface beta-glucosidase involved in cell wall biosynthesis and septation, and thus required for normal growth and correct hyphal morphogenesis. Has hydrolytic activity on linear (1->3)-beta-D-glucans such as laminaribiose and other laminarioligosaccharides. Has also a minor transferase activity. KEYWORDS: Carbohydrate metabolism;Cell wall;Cell wall biogenesis/degradation;Glycoprotein;Glycosidase;Hydrolase;Polysaccharide degradation;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted, cell wall. Secreted. " Q4WLB9,"PROTEIN NAMES: GPI-anchored hemophore cfmA (GPI-anchored CFEM domain protein A) PROTEIN FAMILY: RBT5 family ORGANISM: Aspergillus fumigatus (strain ATCC MYA-4609 / CBS 101355 / FGSC A1100 / Af293) (Neosartorya fumigata) SIMILARITY: Belongs to the RBT5 family. FUNCTION: GPI-anchored cell wall protein involved in stabilizing the cell wall. Not implicated in virulence, heme uptake and biofilm formation. KEYWORDS: Cell membrane;Cell wall;Direct protein sequencing;Disulfide bond;Glycoprotein;GPI-anchor;Heme;Iron;Lipoprotein;Membrane;Metal-binding;Phosphoprotein;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted, cell wall Cell membrane ; Lipid-anchor, GPI-anchor Note=Found anchored in the cell membrane as well as a covalently-linked GPI-modified cell wall protein (GPI-CWP). " Q4WPF5,"PROTEIN NAMES: C2H2 finger domain transcription factor sebA (Stress response element-binding protein A) ORGANISM: Aspergillus fumigatus (strain ATCC MYA-4609 / CBS 101355 / FGSC A1100 / Af293) (Neosartorya fumigata) FUNCTION: Transcription factor that is involved in the response to heat shock, oxidative stress, and poor nutrient conditions. Controls expression of oxidative stress response genes such as ccp1, cat1, cat2, sod2; as well as of heat shock genes such as hsf1, hsp30 and hsp90. Negatively controls the expression of the fumiquinazoline (fmq) cluster via binding to the STRE motifs at the fmqA-D promoters. Plays a role in virulence. KEYWORDS: Cytoplasm;Metal-binding;Nucleus;Reference proteome;Repeat;Stress response;Transcription;Transcription regulation;Virulence;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Cytoplasm Note=Accumulates in the nucleus upon exposure to oxidative stress and heat shock conditions. " Q4WR75,"PROTEIN NAMES: Ferric/cupric reductase transmembrane component B (Ferric reductase B) (Ferrireductase B) (Ferric-chelate reductase B) (Metalloreductase freB) PROTEIN FAMILY: Ferric reductase (FRE) family ORGANISM: Aspergillus fumigatus (strain ATCC MYA-4609 / CBS 101355 / FGSC A1100 / Af293) (Neosartorya fumigata) SIMILARITY: Belongs to the ferric reductase (FRE) family. FUNCTION: Ferric reductase involved in adaptation to iron starvation and which is most likely part of the reductive iron assimilatory system (RIA), a siderophore-independent high affinity iron uptake mechanism. KEYWORDS: Cell membrane;Glycoprotein;Ion transport;Iron;Iron transport;Membrane;Metal-binding;Oxidoreductase;Reference proteome;Signal;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein " Q4WR83,"PROTEIN NAMES: Acyl-CoA ligase sidI (Siderophore biosynthesis protein I) PROTEIN FAMILY: ATP-dependent AMP-binding enzyme family ORGANISM: Aspergillus fumigatus (strain ATCC MYA-4609 / CBS 101355 / FGSC A1100 / Af293) (Neosartorya fumigata) SIMILARITY: Belongs to the ATP-dependent AMP-binding enzyme family. FUNCTION: Acyl-CoA ligase; part of the siderophore biosynthetic pathway. Aspergillus fumigatus produces 4 types of siderophores, low-molecular-mass iron chelators, including excreted fusarinine C (FsC) and triacetylfusarinine C (TAFC) for iron uptake and intacellular ferricrocin (FC) for hyphal and hydroxyferricrocin (HFC) for conidial iron distribution and storage. TAFC consists of 3 N(2)-acetyl-N(5)-anhydromevalonyl-N(5)-hydroxyornithine residues cyclically linked by ester bonds; FC is a cyclic hexapeptide with the structure Gly-Ser-Gly-(N(5)-acetyl-N(5)-hydroxyornithine)x3. The biosynthesis of all four siderophores depends on the hydroxylation of ornithine, catalyzed by the monooxygenase sidA. Subsequently, the pathways for biosynthesis of extra- and intracellular siderophores split. For biosynthesis of extracellular siderophores, the transacylase sidF transfers anhydromevalonyl to N(5)-hydroxyornithine. The required anhydromevalonyl-CoA moiety is derived from mevalonate by CoA ligation and dehydration catalyzed by sidI and sidH respectively. The acetylation of N(5)-hydroxyornithine for FC biosynthesis involves the constitutively expressed sidL. FC is hydroxylated to HFC by an as yet uncharacterized enzyme during conidiation. Assembly of fusarinine C (FsC) and FC is catalyzed by two different nonribosomal peptide synthetases (NRPS), sidD and sidC respectively. Subsequently, sidG catalyzes N2-acetylation of FsC for forming TAFC. Both extra- and intracellular siderophores are crucial for growth during iron limitation and virulence. PATHWAY: Siderophore biosynthesis. KEYWORDS: ATP-binding;Ligase;Nucleotide-binding;Peroxisome;Reference proteome SUBCELLULAR LOCATION: Peroxisome Note=Targeted to peroxisomes via its PTS2-type peroxisomal targeting signal and the corresponding receptor pexG. " Q4WZB3,"PROTEIN NAMES: Heptaketide hydrolyase ayg1 (Conidial pigment biosynthesis protein ayg1) PROTEIN FAMILY: AB hydrolase superfamily ORGANISM: Aspergillus fumigatus (strain ATCC MYA-4609 / CBS 101355 / FGSC A1100 / Af293) (Neosartorya fumigata) SIMILARITY: Belongs to the AB hydrolase superfamily. FUNCTION: Heptaketide hydrolyase; part of the gene cluster that mediates the biosynthesis of dihydroxynaphthalene (DHN)-melanin, a bluish-green pigment and a structural component of the conidial wall. The first step of the pathway is the production of the heptaketide naphtopyrone YWA1 by the polyketide synthase alb1 though condensation of acetyl-CoA with malonyl-CoA. The naphtopyrone YWA1 is then converted to the pentaketide 1,3,6,8-tetrahydroxynaphthalene (1,3,6,8-THN) by the heptaketide hydrolyase ayp1 though chain-length shortening. 1,3,6,8-THN is substrate of the hydroxynaphthalene reductase arp2 to yield scytalone. The scytalone dehydratase arp1 then reduces scytalone to 1,3,8-THN. 1,3,8-THN is also substrate of the hydroxynaphthalene reductase arp2 to yield vermelone. Vermelone is further converted by the multicopper oxidase abr1 to 1,8-DHN. Finally the laccase abr2 transforms 1,8-DHN to DHN-melanin. DHN-melanin biosynthesis appears to be initiated in endosomes where early enzymes (abl1, ayg1, arp1 and arp2) localize, with exocytosis leading to melanin deposition on the cell surface where late enzymes (abr1 and abr2) localize. DHN-melanin is an important structural component of the outer cell wall and is required for the presence of conidial surface hydrophobins. DHN-melanin also plays a crucial role in fungal virulence, including a protective role against the host's immune defenses. DHN-melanin protects also conidia against amoeba predation. PATHWAY: Pigment biosynthesis; melanin biosynthesis. KEYWORDS: Endosome;Hydrolase;Reference proteome SUBCELLULAR LOCATION: Endosome " Q4X1W0,"PROTEIN NAMES: 4'-phosphopantetheinyl transferase A (PPTase A) (Acyl-carrier-protein synthase pptA) (Phosphopantetheine:protein transferase pptA) PROTEIN FAMILY: P-Pant transferase superfamily ORGANISM: Aspergillus fumigatus (strain ATCC MYA-4609 / CBS 101355 / FGSC A1100 / Af293) (Neosartorya fumigata) SIMILARITY: Belongs to the P-Pant transferase superfamily. FUNCTION: Acyl-carrier-protein synthase that transfers the 4'-phosphopantetheine moiety from coenzyme A to a Ser of an acyl-carrier-protein. The 4'-phosphopantetheine (4'-PPT) portion of CoA provides the essential prosthetic group for a number of carrier proteins and multi-domain enzymes, priming them for the acceptance of acyl building blocks in fatty acid synthesis and many aspects of secondary metabolism mediated by polyketide synthases (PKSs) and non-ribosomal peptide synthetases (NRPSs). PptA is able to transfer the cofactor to a broad range of enzymes with acyl- or peptidyl-carrier protein domains and activates target enzymes involved in the synthesis of lysine, but also secondary metabolites including gliotoxin, fumigaclavine C, fumiquinazole A, fumiquinazoline C, pyripyroprene A, fumagillin, the siderophores triacetylfusarinine C (TAFC) and ferricrocin (FC), and dihydroxy naphthalene (DHN)-melanin. Plays an essential role in virulence. KEYWORDS: Reference proteome;Transferase;Virulence " Q500U8,"PROTEIN NAMES: Tetraketide alpha-pyrone reductase 1 (Protein DIHYDROFLAVONOL 4-REDUCTASE-LIKE 1) PROTEIN FAMILY: NAD(P)-dependent epimerase/dehydratase family, Dihydroflavonol-4-reductase subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the NAD(P)-dependent epimerase/dehydratase family. Dihydroflavonol-4-reductase subfamily. FUNCTION: Involved in the biosynthesis of hydroxylated tetraketide compounds that serve as sporopollenin precursors (the main constituents of exine). Is essential for pollen wall development. Acts on tetraketide alpha-pyrones and reduces the carbonyl function on the tetraketide alkyl chain to a secondary alcohol function. KEYWORDS: Cytoplasm;Endoplasmic reticulum;NADP;Nucleus;Oxidoreductase;Reference proteome SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Endoplasmic reticulum Note=According to previous research, TKPR1 is associated with the endoplasmic reticulum. " Q500V5,"PROTEIN NAMES: Protein AGENET DOMAIN (AGD)-CONTAINING P1 (Protein ONE AGENET DOMAIN-CONTAINING PROTEIN) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Heterochromatin-binding protein that preferentially occupies long transposons and specifically recognizes the histone H3 'Lys-9' methylation (H3K9me) marks, with a stronger affinity for dimethylated H3K9 (H3K9me2). Required for transcriptional silencing, non-CG DNA methylation (e.g. CHG and CHH regions), and H3K9 dimethylation (H3K9me2) at some loci. Mediates heterochromatin phase separation and chromocenter formation. KEYWORDS: 3D-structure;Chromatin regulator;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus Note=Enriched in heterochromatin, concentrated in centromeric and pericentromeric regions. " Q500Y9,"PROTEIN NAMES: NADH kinase (AtNADK-3) PROTEIN FAMILY: NAD kinase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the NAD kinase family. FUNCTION: Phosphorylates specifically NADH. Can phosphorylate NAD with a 100-fold decrease in efficiency compared to NADH. Prefers ATP as nucleoside triphosphate substrate. Can also utilize UTP, GTP and CTP. Key source of the cellular reductant NADPH which is an important antioxidant factor. KEYWORDS: ATP-binding;Cytoplasm;Kinase;NAD;NADP;Nucleotide-binding;Reference proteome;Transferase SUBCELLULAR LOCATION: Cytoplasm " Q501D5,PROTEIN NAMES: Mini-chromosome maintenance complex-binding protein (MCM-BP) (MCM-binding protein) (Protein E2F TARGET GENE 1) PROTEIN FAMILY: MCMBP family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the MCMBP family. FUNCTION: Associated component of the MCM complex that acts as a regulator of DNA replication. Binds to the MCM complex during late S phase and may act by promoting the disassembly of the MCM complex from chromatin. Required for sister chromatid cohesion. KEYWORDS: Cell cycle;Cell division;DNA replication;Mitosis;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus Note=Associates with the replisome complex. Q501Z5,"PROTEIN NAMES: tRNA modification GTPase GTPBP3, mitochondrial (GTP-binding protein 3) PROTEIN FAMILY: TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily, TrmE GTPase family ORGANISM: Danio rerio (Zebrafish) (Brachydanio rerio) SIMILARITY: Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. TrmE GTPase family. FUNCTION: GTPase involved in the 5-carboxymethylaminomethyl modification (mnm(5)s(2)U34) of the wobble uridine base in mitochondrial tRNAs. KEYWORDS: GTP-binding;Mitochondrion;Nucleotide-binding;Reference proteome;Transit peptide;tRNA processing SUBCELLULAR LOCATION: Mitochondrion " Q504J1,"PROTEIN NAMES: Caspase b (Caspase 19a) [Cleaved into: Caspase b subunit p20; Caspase b subunit p10] PROTEIN FAMILY: Peptidase C14A family ORGANISM: Danio rerio (Zebrafish) (Brachydanio rerio) SIMILARITY: Belongs to the peptidase C14A family. FUNCTION: Thiol protease which cleaves IL-1 beta (il1b), releasing the mature cytokine which is involved in a variety of inflammatory processes, and mediates apoptosis. Component of the NLRP1 inflammasome, which plays a crucial role in innate immunity and inflammation. In response to pathogens and other damage-associated signals, recruited to the NLRP1 inflammasome in its precursor form following the recruitment of caspase caspa. Its subsequent activation causes the cleavage of the midformed pro-il1b and results in il1b maturation and secretion in the extracellular milieu. Activated by direct binding to bacterial lipopolysaccharides (LPS), which causes non-canonical inflammasome activation and results in the pyroptosis of infected cells and their extrusion into the gut lumen, as well as in cytokine secretion. Plays a crucial role in the restriction of bacterial infection to intestinal sites. Pyroptosis limits bacterial replication, while cytokine secretion promotes the recruitment and activation of immune cells and triggers mucosal inflammation (By similarity). Promotes pyroptosis by bacterial infection by E.piscicida. KEYWORDS: Apoptosis;Cytoplasm;Hydrolase;Immunity;Inflammasome;Inflammatory response;Innate immunity;Necrosis;Protease;Reference proteome;Thiol protease;Zymogen SUBCELLULAR LOCATION: Inflammasome Cytoplasm Note=Co-localizes with pycard, caspa and nlrp1 in the cytoplasm. Co-localizes with pycard at large cytoplasmic aggregates, known as specks. " Q50925,PROTEIN NAMES: Hydroxylamine oxidoreductase (HAO) ORGANISM: Nitrosomonas europaea (strain ATCC 19718 / CIP 103999 / KCTC 2705 / NBRC 14298) FUNCTION: Catalyzes the oxidation of hydroxylamine to nitrite. The electrons released in the reaction are partitioned to ammonium monooxygenase and to the respiratory chain. The immediate acceptor of electrons from HAO is cytochrome c-554. KEYWORDS: 3D-structure;Direct protein sequencing;Heme;Iron;Metal-binding;Oxidoreductase;Reference proteome;Signal SUBCELLULAR LOCATION: Anammoxosome Q50L41,"PROTEIN NAMES: Cytosolic phospholipase A2 zeta (cPLA2-zeta) (Phospholipase A2 group IVF) ORGANISM: Mus musculus (Mouse) FUNCTION: Has calcium-dependent phospholipase and lysophospholipase activities with a potential role in membrane lipid remodeling and biosynthesis of lipid mediators. Preferentially hydrolyzes the ester bond of the fatty acyl group attached at sn-2 position of phospholipids (phospholipase A2 activity). Selectively hydrolyzes sn-2 arachidonoyl group from membrane phospholipids, providing the precursor for eicosanoid biosynthesis. In myocardial mitochondria, plays a major role in arachidonate release that is metabolically channeled to the formation of cardioprotective eicosanoids, epoxyeicosatrienoates (EETs) (By similarity). KEYWORDS: Calcium;Cell membrane;Cytoplasm;Hydrolase;Lipid degradation;Lipid metabolism;Membrane;Metal-binding;Mitochondrion;Phospholipid degradation;Phospholipid metabolism;Reference proteome SUBCELLULAR LOCATION: Cytoplasm, cytosol Cell membrane ; Peripheral membrane protein Mitochondrion " Q51693,"PROTEIN NAMES: Capsule biosynthesis protein CapD proenzyme [Cleaved into: Capsule biosynthesis protein CapD large chain; Capsule biosynthesis protein CapD small chain] PROTEIN FAMILY: Gamma-glutamyltransferase family ORGANISM: Bacillus anthracis SIMILARITY: Belongs to the gamma-glutamyltransferase family. FUNCTION: Transpeptidase that cleaves the poly-gamma-D-glutamate capsule and catalyzes the formation of an amide bond with the side-chain amino group of meso-diaminopimelic acid (m-DAP) in the peptidoglycan scaffold. Degradation of the high-molecular weight capsule (H-capsule) to the lower-molecular weight capsule (L-capsule), which is released from the bacterial cell surface. The production of L-capsule is essential to mediate escape from host defenses. PATHWAY: Capsule biogenesis; capsule polysaccharide biosynthesis. KEYWORDS: 3D-structure;Capsule biogenesis/degradation;Direct protein sequencing;Hydrolase;Plasmid;Protease;Reference proteome;Signal;Transferase;Virulence;Zymogen " Q51945,"PROTEIN NAMES: Tartrate dehydrogenase/decarboxylase (TDH) (D-malate dehydrogenase [decarboxylating]) PROTEIN FAMILY: Isocitrate and isopropylmalate dehydrogenases family ORGANISM: Pseudomonas putida (Arthrobacter siderocapsulatus) SIMILARITY: Belongs to the isocitrate and isopropylmalate dehydrogenases family. FUNCTION: Has multiple catalytic activities. Apart from catalyzing the oxidation of (+)-tartrate to oxaloglycolate, also converts meso-tartrate to D-glycerate and catalyzes the oxidative decarboxylation of D-malate to pyruvate. PATHWAY: Carbohydrate acid metabolism; tartrate degradation; 2-hydroxy-3-oxosuccinate from L-tartrate: step 1/1.; PATHWAY: Carbohydrate acid metabolism; tartrate degradation; 2-hydroxy-3-oxosuccinate from meso-tartrate: step 1/1.; PATHWAY: Carbohydrate acid metabolism; tartrate degradation; D-glycerate from L-tartrate: step 1/1. KEYWORDS: 3D-structure;Cytoplasm;Direct protein sequencing;Lyase;Magnesium;Manganese;Metal-binding;NAD;Oxidoreductase SUBCELLULAR LOCATION: Cytoplasm. " Q51WZ9,"PROTEIN NAMES: Autophagy-related protein 9 PROTEIN FAMILY: ATG9 family ORGANISM: Pyricularia oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast fungus) (Magnaporthe oryzae) SIMILARITY: Belongs to the ATG9 family. FUNCTION: Phospholipid scramblase involved in autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation. Cycles between the preautophagosomal structure/phagophore assembly site (PAS) and the cytoplasmic vesicle pool and supplies membrane for the growing autophagosome. Lipid scramblase activity plays a key role in preautophagosomal structure/phagophore assembly by distributing the phospholipids that arrive through ATG2 from the cytoplasmic to the luminal leaflet of the bilayer, thereby driving autophagosomal membrane expansion. Required for mitophagy. Also involved in endoplasmic reticulum-specific autophagic process and is essential for the survival of cells subjected to severe ER stress (By similarity). Different machineries are required for anterograde trafficking to the PAS during either the Cvt pathway or bulk autophagy and for retrograde trafficking. Plays a role in appressorium formation and pathogenicity. KEYWORDS: Acetylation;Autophagy;Cytoplasmic vesicle;Endoplasmic reticulum;Germination;Glycoprotein;Golgi apparatus;Lipid transport;Membrane;Phosphoprotein;Reference proteome;Stress response;Transmembrane;Transmembrane helix;Transport;Vacuole;Virulence SUBCELLULAR LOCATION: Preautophagosomal structure membrane ; Multi-pass membrane protein Cytoplasmic vesicle membrane ; Multi-pass membrane protein Vacuole membrane ; Multi-pass membrane protein Golgi apparatus membrane ; Multi-pass membrane protein Endoplasmic reticulum membrane ; Multi-pass membrane protein Note=Concurrent with the cytoplasmic punctation, localizes on the central vacuole of the submerged hyphae from the conidia cultured in rich media. ATG1, ATG2 and ATG18, but not ATG13, are required for ATG9-cycling through the multiple localization sites. " Q52085,"PROTEIN NAMES: Cell-cell adhesion glycoprotein 64 (Gp64) (Contact site 1) ORGANISM: Heterostelium pallidum (Cellular slime mold) (Polysphondylium pallidum) FUNCTION: Cell-cell adhesion during development. KEYWORDS: Cell adhesion;Cell membrane;Developmental protein;Direct protein sequencing;Disulfide bond;Glycoprotein;GPI-anchor;Lipoprotein;Membrane;Signal SUBCELLULAR LOCATION: Cell membrane ; Lipid-anchor, GPI-anchor Note=Attached to the membrane by a GPI-like-anchor that contains a phosphoceramide group. MISCELLANEOUS: Loss-of-function mutant (antisense inhibition) shows reduced cell adhesiveness and forms smaller aggregates than wild-type, though it does complete development and produce fruiting bodies." Q52430,"PROTEIN NAMES: Cysteine protease avirulence protein AvrPphB [Cleaved into: 7 kDa product; 28 kDa product] PROTEIN FAMILY: Peptidase C58 family ORGANISM: Pseudomonas savastanoi pv. phaseolicola (Pseudomonas syringae pv. phaseolicola) SIMILARITY: Belongs to the peptidase C58 family. FUNCTION: Cysteine protease avirulence protein, which is essential during infection of plant cells from cultivar-specific of beans and Arabidopsis thaliana. The autocleavage of the protein is required for virulence function. May act by affecting the plant defense system. In plants lacking R3 or RPS5 resistance genes, it probably impairs the plant defense system and leads to the bacteria multiplication. In contrast, in plants containing the R3 or RPS5 protein, it is unable to induce disease symptoms, explaining its avirulence name. The 7 kDa product is required for the type-III translocation from Pseudomonas strains to the plant, but are partially dispensable for effector recognition following in planta expression. In infected plants, it acts by cleaving the PBS1 protein, which leads to resistance or disease, depending on the presence or absence of RPS5, respectively. Targets the Arabidopsis kinases PBS1, BIK1, PBL1, PBL2, PBL3, PBL5, PBL7, PBL9 and PBL11 for cleavage in vitro. Can block recognition of AvrB avirulence factor by plant cells by cleaving Arabidopsis RIPK kinase and suppressing Arabidopsis RPM1 activation. Cannot block AvrRpm1-induced activation of RPM1. KEYWORDS: 3D-structure;Autocatalytic cleavage;Host membrane;Hydrolase;Hypersensitive response elicitation;Lipoprotein;Membrane;Myristate;Protease;Secreted;Thiol protease;Virulence SUBCELLULAR LOCATION: Secreted Host membrane Note=In infected plant cells, it is membrane-associated. " Q52472,"PROTEIN NAMES: D-threo-aldose 1-dehydrogenase (L-fucose dehydrogenase) PROTEIN FAMILY: Aldo/keto reductase family ORGANISM: Pseudomonas sp SIMILARITY: Belongs to the aldo/keto reductase family. FUNCTION: Catalyzes the oxidation of L-fucose to L-fuconolactone in the presence of NADP(+). Also active against L-galactose and, to a much lesser degree, D-arabinose. Uses NADP(+) as a hydrogen acceptor much more efficiently than NAD(+). KEYWORDS: Direct protein sequencing;NAD;NADP;Oxidoreductase " Q52EB3,"PROTEIN NAMES: Serine/threonine-protein kinase ATG1 (Autophagy-related protein 1) PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family, APG1/unc-51/ULK1 subfamily ORGANISM: Pyricularia oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast fungus) (Magnaporthe oryzae) SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily. FUNCTION: Serine/threonine protein kinase involved in the cytoplasm to vacuole transport (Cvt) and found to be essential in autophagy, where it is required for the formation of autophagosomes. Involved in the clearance of protein aggregates which cannot be efficiently cleared by the proteasome. Required for selective autophagic degradation of the nucleus (nucleophagy) as well as for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Also involved in endoplasmic reticulum-specific autophagic process, in selective removal of ER-associated degradation (ERAD) substrates. Plays a key role in ATG9 and ATG23 cycling through the pre-autophagosomal structure and is necessary to promote ATG18 binding to ATG9 through phosphorylation of ATG9. Catalyzes phosphorylation of ATG4, decreasing the interaction between ATG4 and ATG8 and impairing deconjugation of PE-conjugated forms of ATG8 (By similarity). Autophagy is essential to fungal development, production of appressorium turgor, and pathogenicity in rice blast disease. KEYWORDS: ATP-binding;Autophagy;Cytoplasm;Kinase;Membrane;Nucleotide-binding;Protein transport;Reference proteome;Serine/threonine-protein kinase;Transferase;Transport SUBCELLULAR LOCATION: Cytoplasm Preautophagosomal structure membrane ; Peripheral membrane protein " Q52S86,"PROTEIN NAMES: Podocalyxin (Gp135) (Podocalyxin-like protein 1) (PC) (PC-like protein 1) (PCLP-1) (cPCLP1) PROTEIN FAMILY: Podocalyxin family ORGANISM: Canis lupus familiaris (Dog) (Canis familiaris) SIMILARITY: Belongs to the podocalyxin family. FUNCTION: Involved in the regulation of both adhesion and cell morphology and cancer progression. Functions as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up initial epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR-dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells. KEYWORDS: Cell adhesion;Cell membrane;Cell projection;Direct protein sequencing;Glycoprotein;Membrane;Phosphoprotein;Reference proteome;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Apical cell membrane. Membrane raft. Cell projection, lamellipodium Cell projection, filopodium Cell projection, ruffle Cell projection, microvillus Membrane ; Single-pass type I membrane protein Note=Forms granular, punctuated pattern, forming patches, preferentially adopting a polar distribution, located on the migrating poles of the cell or forming clusters along the terminal ends of filipodia establishing contact with the endothelial cells. Colocalizes with the submembrane actin of lamellipodia, particularly associated with ruffles. Colocalizes with vinculin at protrusions of cells. Colocalizes with ITGB1. Colocalizes with actin filaments, ezrin and NHERF1 in a punctate pattern at the apical cell surface where microvilli form. Colocalizes with EZR and NHERF2 at the apical cell membrane of glomerular epithelium cells (By similarity). In single attached epithelial cells is restricted to a preapical pole on the free plasma membrane whereas other apical and basolateral proteins are not yet polarized. Colocalizes with NHERF2 at the apical plasma membrane during epithelial polarization. Colocalizes with NHERF1 at the trans-Golgi network (transiently) and at the apical plasma membrane. Its association with the membrane raft is transient. Colocalizes with PARD3, PRKCI, EXOC5, OCLN, RAB11A and RAB8A in apical membrane initiation sites (AMIS) during the generation of apical surface and luminogenesis (By similarity). " Q53692,"PROTEIN NAMES: O-aminophenol oxidase (Phenoxazinone synthase) (PHS) PROTEIN FAMILY: Multicopper oxidase family ORGANISM: Streptomyces antibioticus SIMILARITY: Belongs to the multicopper oxidase family. FUNCTION: Could be involved in the spore pigmentation and melanin production. Catalyzes the oxidative coupling of 2-aminophenols to form the 2-aminophenoxazinone chromophore. 2-aminophenoxazinone synthesis proceeds via a sequence of three consecutive 2-electron aminophenol oxidations. First, the o-aminophenol is oxidized by two electrons to the quinone imine, which then conjugates to a second o-aminophenol molecule while still bound to the enzyme. This product is further oxidized by two electrons to give rise to the p-quinone imine. The last two steps of the reaction, another conjugation to generate the tricyclic structure and a final two-electron oxidation to yield the 2-aminophenoxazinone product, are thought to be non-enzymatic. It can also uuse 3-hydroxyanthranilic acid (HAA), 4-methyl-3-hydroxyanthranilic acid (MHA), 3,4-dihydroxy-L-phenylalanine (L-DOPA), ferrocyanide and thiophenol as substrates. KEYWORDS: 3D-structure;Antibiotic biosynthesis;Copper;Direct protein sequencing;Metal-binding;Oxidoreductase;Repeat " Q53EK2,PROTEIN NAMES: Non-structural maintenance of chromosomes element 1 (Non-SMC element 1) PROTEIN FAMILY: NSE1 family ORGANISM: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) SIMILARITY: Belongs to the NSE1 family. FUNCTION: Acts in a DNA repair pathway for removal of UV-induced DNA damage that is distinct from classical nucleotide excision repair and in repair of ionizing radiation damage. Functions in homologous recombination repair of DNA double strand breaks and in recovery of stalled replication forks. Plays a critical role in meiosis. KEYWORDS: Direct protein sequencing;DNA damage;DNA recombination;DNA repair;Meiosis;Metal-binding;Nucleus;Reference proteome;Transferase;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Q53EL9,PROTEIN NAMES: Seizure protein 6 homolog (SEZ-6) (hSEZ-6) PROTEIN FAMILY: SEZ6 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the SEZ6 family. FUNCTION: May play a role in cell-cell recognition and in neuronal membrane signaling. Seems to be important for the achievement of the necessary balance between dendrite elongation and branching during the elaboration of a complex dendritic arbor. Involved in the development of appropriate excitatory synaptic connectivity (By similarity). KEYWORDS: Alternative splicing;Cell membrane;Disulfide bond;Glycoprotein;Membrane;Reference proteome;Repeat;Signal;Sushi;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein Note=Localized on dendrites and in the synaptic and postsynaptic fraction. Q53FA7,"PROTEIN NAMES: Quinone oxidoreductase PIG3 (NADPH:quinone reductase PIG3) (Tumor protein p53-inducible protein 3) (Protein PIG3) (p53-induced gene 3 protein) PROTEIN FAMILY: Zinc-containing alcohol dehydrogenase family, Quinone oxidoreductase subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily. FUNCTION: Catalyzes the NADPH-dependent reduction of quinones. Exhibits a low enzymatic activity with beta-naphthoquinones, with a strong preference for the ortho-quinone isomer (1,2-beta-naphthoquinone) over the para isomer (1,4-beta-naphthoquinone). Also displays a low reductase activity for non-quinone compounds such as diamine and 2,6-dichloroindophenol (in vitro). Involved in the generation of reactive oxygen species (ROS). KEYWORDS: 3D-structure;Acetylation;Alternative splicing;Direct protein sequencing;NADP;Oxidoreductase;Reference proteome MISCELLANEOUS: [Isoform 1]: Major isoform under normal light conditions.; MISCELLANEOUS: [Isoform 2]: Major isoform under UV light exposure. Undergoes rapid proteolytic degradation by the proteasome." Q53FZ2,"PROTEIN NAMES: Acyl-coenzyme A synthetase ACSM3, mitochondrial (Acyl-CoA synthetase medium-chain family member 3) (Butyrate--CoA ligase 3) (Butyryl-coenzyme A synthetase 3) (Middle-chain acyl-CoA synthetase 3) (Propionate--CoA ligase) (Protein SA homolog) PROTEIN FAMILY: ATP-dependent AMP-binding enzyme family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the ATP-dependent AMP-binding enzyme family. FUNCTION: Catalyzes the activation of fatty acids by CoA to produce an acyl-CoA, the first step in fatty acid metabolism. Capable of activating medium-chain fatty acids with a preference for isobutyrate among fatty acids with 2-6 carbon atoms (By similarity). KEYWORDS: Acetylation;Alternative splicing;ATP-binding;Fatty acid metabolism;Ligase;Lipid metabolism;Magnesium;Metal-binding;Mitochondrion;Nucleotide-binding;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Mitochondrion Mitochondrion matrix " Q53GS9,"PROTEIN NAMES: Ubiquitin carboxyl-terminal hydrolase 39 (SAD1 homolog) (U4/U6.U5 tri-snRNP-associated 65 kDa protein) PROTEIN FAMILY: Peptidase C19 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the peptidase C19 family. FUNCTION: Deubiquitinating enzyme that plays a role in many cellular processes including cellular antiviral response, epithelial morphogenesis, DNA repair or B-cell development. Plays a role in pre-mRNA splicing as a component of the U4/U6-U5 tri-snRNP, one of the building blocks of the precatalytic spliceosome. Specifically regulates immunoglobulin gene rearrangement in a spliceosome-dependent manner, which involves modulating chromatin interactions at the Igh locus and therefore plays an essential role in B-cell development (By similarity). Regulates AURKB mRNA levels, and thereby plays a role in cytokinesis and in the spindle checkpoint. Regulates apoptosis and G2/M cell cycle checkpoint in response to DNA damage by deubiquitinating and stabilizing CHK2. Plays also an important role in DNA repair by controlling the recruitment of XRCC4/LIG4 to DNA double-strand breaks for non-homologous end-joining repair. Participates in antiviral activity by affecting the type I IFN signaling by stabilizing STAT1 and decreasing its 'Lys-6'-linked ubiquitination. Contributes to non-canonical Wnt signaling during epidermal differentiation (By similarity). Acts as a negative regulator NF-kappa-B activation through deubiquitination of 'Lys-48'-linked ubiquitination of NFKBIA. KEYWORDS: 3D-structure;Alternative splicing;Cell cycle;Cell division;Hydrolase;Isopeptide bond;Metal-binding;mRNA processing;mRNA splicing;Nucleus;Phosphoprotein;Reference proteome;Spliceosome;Ubl conjugation;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus " Q53TZ2,"PROTEIN NAMES: L-arabinose 1-dehydrogenase (NAD(P)(+)) (D-galactose 1-dehydrogenase) PROTEIN FAMILY: Gfo/Idh/MocA family ORGANISM: Azospirillum brasilense SIMILARITY: Belongs to the Gfo/Idh/MocA family. FUNCTION: Catalyzes the NAD(P)(+)-dependent conversion of L-arabinose to L-arabino-gamma-lactone. Is involved in a degradation pathway of L-arabinose that allows A.brasilense to grow on L-arabinose as a sole carbon source. Prefers NADP(+) to NAD(+) as electron acceptor. Displays high catalytic efficiency for both L-arabinose and D-galactose in vitro. However, the enzyme appears to be involved in the metabolism of L-arabinose but not D-galactose in vivo. To a lesser extent, is also active on D-talose and D-xylose as substrates in vitro, but not with D-arabinose, D-glucose, D-ribose, L-xylose, L-mannose, L-lyxose, and D-fructose. PATHWAY: Carbohydrate degradation; L-arabinose degradation via L-arabinono-1,4-lactone pathway. KEYWORDS: 3D-structure;Arabinose catabolism;Carbohydrate metabolism;Direct protein sequencing;NAD;NADP;Oxidoreductase " Q53VY2,"PROTEIN NAMES: CRISPR-associated endonuclease/helicase Cas3 (CRISPR-associated ssDNA endonuclease/helicase Cas3) PROTEIN FAMILY: CRISPR-associated nuclease Cas3-HD family; CRISPR-associated helicase Cas3 family ORGANISM: Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) SIMILARITY: In the N-terminal section; belongs to the CRISPR-associated nuclease Cas3-HD family.; SIMILARITY: In the central section; belongs to the CRISPR-associated helicase Cas3 family. FUNCTION: CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Cas3 plus Cascade participate in CRISPR interference, the third stage of CRISPR immunity. The N-terminal domain (residues 6-260) acts as a ssDNA endonuclease, has no activity on dsDNA. KEYWORDS: 3D-structure;Antiviral defense;ATP-binding;Endonuclease;Helicase;Hydrolase;Metal-binding;Nuclease;Nucleotide-binding;Plasmid;Reference proteome " Q54089,"PROTEIN NAMES: Bifunctional (p)ppGpp synthase/hydrolase RelA [Includes: GTP pyrophosphokinase ((p)ppGpp synthase) (ATP:GTP 3'-pyrophosphotransferase) (Stringent response-like protein) (ppGpp synthase I); Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (Penta-phosphate guanosine-3'-pyrophosphohydrolase) ((ppGpp)ase)] PROTEIN FAMILY: RelA/SpoT family ORGANISM: Streptococcus dysgalactiae subsp. equisimilis (Streptococcus equisimilis) SIMILARITY: Belongs to the RelA/SpoT family. FUNCTION: In eubacteria ppGpp (guanosine 3'-diphosphate 5'-diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes both the formation of pppGpp which is then hydrolyzed to form ppGpp, and the hydrolysis of ppGpp. The enzyme does not simultaneously display both synthase and hydrolase activities. In the structure of residues 1-385 there are 2 conformations seen, the hydrolase-OFF/synthase-ON and hydrolase-ON/synthase-OFF, suggesting there is ligand-induced signal transmission between the 2 active sites. PATHWAY: Purine metabolism; ppGpp biosynthesis; ppGpp from GDP: step 1/1.; PATHWAY: Purine metabolism; ppGpp biosynthesis; ppGpp from GTP: step 1/2. KEYWORDS: 3D-structure;ATP-binding;GTP-binding;Hydrolase;Kinase;Magnesium;Manganese;Metal-binding;Nucleotide-binding;Transferase " Q54530,"PROTEIN NAMES: Aklavinone 12-hydroxylase RdmE (Aklavinone 11-hydroxylase) PROTEIN FAMILY: PheA/TfdB FAD monooxygenase family ORGANISM: Streptomyces purpurascens SIMILARITY: Belongs to the PheA/TfdB FAD monooxygenase family. FUNCTION: Involved in the biosynthesis of the anthracyclines carminomycin, rhodomycin and daunorubicin (daunomycin) which are aromatic polyketide antibiotics that exhibit high cytotoxicity and are widely applied in the chemotherapy of a variety of cancers. Catalyzes the incorporation of a hydroxyl group at position C-11 of aklavinone, resulting in epsilon-rhodomycinone. It cannot accept substrates glycosylated at position C-7 and is specific for the C-9R configuration of anthracyclines. It can use both NAD or NADP but it is slowly inactivated in the presence of NADH. PATHWAY: Antibiotic biosynthesis; daunorubicin biosynthesis.; PATHWAY: Antibiotic biosynthesis; carminomycin biosynthesis.; PATHWAY: Antibiotic biosynthesis; rhodomycin biosynthesis. KEYWORDS: 3D-structure;Antibiotic biosynthesis;Direct protein sequencing;FAD;Flavoprotein;NAD;NADP;Nucleotide-binding;Oxidoreductase " Q54735,"PROTEIN NAMES: Carbonic anhydrase (Carbonate dehydratase) PROTEIN FAMILY: Beta-class carbonic anhydrase family ORGANISM: Synechocystis sp. (strain PCC 6803 / Kazusa) SIMILARITY: Belongs to the beta-class carbonic anhydrase family. FUNCTION: Reversible hydration of carbon dioxide. Essential to photosynthetic carbon dioxide fixation, supplies CO(2) to RuBisCO (ribulose bisphosphate carboxylase, rbcL-rbcS) in the carboxysome. KEYWORDS: 3D-structure;Bacterial microcompartment;Carbon dioxide fixation;Carboxysome;Lyase;Metal-binding;Photosynthesis;Reference proteome;Zinc SUBCELLULAR LOCATION: Carboxysome Note=This cyanobacterium makes beta-type carboxysomes. Associates with the shell portion of carboxysomes. " Q54AX5,"PROTEIN NAMES: Leucine-rich repeat protein lrrA ORGANISM: Dictyostelium discoideum (Social amoeba) FUNCTION: Involved in cytoskeleton remodeling, which is needed for normal chemotactic aggregation and efficient cell sorting during multicellular morphogenesis. KEYWORDS: Cytoplasm;Leucine-rich repeat;Reference proteome;Repeat SUBCELLULAR LOCATION: Cytoplasm " Q54CK6,"PROTEIN NAMES: Vesicle transport through interaction with t-SNAREs homolog 1A PROTEIN FAMILY: VTI1 family ORGANISM: Dictyostelium discoideum (Social amoeba) SIMILARITY: Belongs to the VTI1 family. FUNCTION: V-SNARE that mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane. These interactions are proposed to mediate aspects of the specificity of vesicle trafficking and to promote fusion of the lipid bilayers. KEYWORDS: Coiled coil;Cytoplasmic vesicle;Direct protein sequencing;Endoplasmic reticulum;Endosome;Membrane;Protein transport;Reference proteome;Signal-anchor;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Membrane ; Single-pass type IV membrane protein Cytoplasmic vesicle, secretory vesicle membrane ; Single-pass type IV membrane protein Cytoplasmic vesicle, clathrin-coated vesicle membrane ; Single-pass type IV membrane protein Endosome membrane ; Single-pass type IV membrane protein Endoplasmic reticulum membrane ; Single-pass type IV membrane protein " Q54D73,"PROTEIN NAMES: Flavohemoprotein B (DdFHb) (Flavohemoglobin B) (Hemoglobin-like protein B) (Nitric oxide dioxygenase B) (NO oxygenase B) (NOD B) PROTEIN FAMILY: Globin family, Two-domain flavohemoproteins subfamily; Flavoprotein pyridine nucleotide cytochrome reductase family ORGANISM: Dictyostelium discoideum (Social amoeba) SIMILARITY: Belongs to the globin family. Two-domain flavohemoproteins subfamily.; SIMILARITY: In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. FUNCTION: Is involved in NO detoxification in an aerobic process, termed nitric oxide dioxygenase (NOD) reaction that utilizes O(2) and NAD(P)H to convert NO to nitrate, which protects the cell from various noxious nitrogen compounds. Therefore, plays a central role in the inducible response to nitrosative stress.; FUNCTION: In the presence of oxygen and NADH, it has NADH oxidase activity, which leads to the generation of superoxide and H(2)O(2). Under anaerobic conditions, it also exhibits nitric oxide reductase and FAD reductase activities. However, all these reactions are much lower than NOD activity (By similarity). KEYWORDS: Cytoplasm;Detoxification;FAD;Flavoprotein;Heme;Iron;Metal-binding;NAD;NADP;Oxidoreductase;Oxygen transport;Reference proteome;Transport SUBCELLULAR LOCATION: Cytoplasm " Q54DD0,"PROTEIN NAMES: AMP deaminase (AMPD1) PROTEIN FAMILY: Metallo-dependent hydrolases superfamily, Adenosine and AMP deaminases family ORGANISM: Dictyostelium discoideum (Social amoeba) SIMILARITY: Belongs to the metallo-dependent hydrolases superfamily. Adenosine and AMP deaminases family. FUNCTION: Catalyzes the conversion of adenosine monophosphate (AMP) to inosine monophosphate (IMP) and ammonia (NH4(+)). Participates in the regulation of the adenylated nucleotide pool and the interconversion to guanylated nucleotides during early morphodifferentiation. PATHWAY: Purine metabolism; IMP biosynthesis via salvage pathway; IMP from AMP: step 1/1. KEYWORDS: Cytoplasm;Developmental protein;Differentiation;Hydrolase;Metal-binding;Nucleotide metabolism;Reference proteome;Sporulation;Zinc SUBCELLULAR LOCATION: Cytoplasm " Q54DE8,"PROTEIN NAMES: Presenilin-B (PS-B) PROTEIN FAMILY: Peptidase A22A family ORGANISM: Dictyostelium discoideum (Social amoeba) SIMILARITY: Belongs to the peptidase A22A family. FUNCTION: Probable catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors. Requires the other members of the gamma-secretase complex to have a protease activity (By similarity). KEYWORDS: Endoplasmic reticulum;Glycoprotein;Golgi apparatus;Hydrolase;Membrane;Notch signaling pathway;Protease;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein Golgi apparatus membrane ; Multi-pass membrane protein " Q54ER9,"PROTEIN NAMES: Basic-leucine zipper transcription factor B PROTEIN FAMILY: BZIP family ORGANISM: Dictyostelium discoideum (Social amoeba) SIMILARITY: Belongs to the bZIP family. FUNCTION: Transcriptional regulator involved in DIF-1 signaling. DIF-1 (Differentiation Inducing Factor-1) is a signal molecule involved in the differentiation of pstO (prestalk-O) cells (By similarity). May be a direct activator of ecmA. KEYWORDS: Coiled coil;DNA-binding;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus Note=In response to DIF-1, it accumulates rapidly in the nucleus. " Q54ET2,"PROTEIN NAMES: Presenilin-A (PS-A) PROTEIN FAMILY: Peptidase A22A family ORGANISM: Dictyostelium discoideum (Social amoeba) SIMILARITY: Belongs to the peptidase A22A family. FUNCTION: Probable catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors. Requires the other members of the gamma-secretase complex to have a protease activity (By similarity). KEYWORDS: Endoplasmic reticulum;Golgi apparatus;Hydrolase;Membrane;Notch signaling pathway;Protease;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein Golgi apparatus membrane ; Multi-pass membrane protein " Q54F25,PROTEIN NAMES: Battenin (Protein CLN3 homolog) PROTEIN FAMILY: Battenin family ORGANISM: Dictyostelium discoideum (Social amoeba) SIMILARITY: Belongs to the battenin family. KEYWORDS: Glycoprotein;Lysosome;Membrane;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Lysosome membrane ; Multi-pass membrane protein Q54GT9,"PROTEIN NAMES: Autophagy protein 5 PROTEIN FAMILY: ATG5 family ORGANISM: Dictyostelium discoideum (Social amoeba) SIMILARITY: Belongs to the ATG5 family. FUNCTION: Involved in autophagic vesicle formation. Conjugation with atg12, through a ubiquitin-like conjugating system involving atg7 as an E1-like activating enzyme and atg10 as an E2-like conjugating enzyme, is essential for its function. The atg12-atg5 conjugate acts as an E3-like enzyme which is required for lipidation of atg8 and its association to the vesicle membranes (By similarity). KEYWORDS: Autophagy;Cytoplasm;Isopeptide bond;Membrane;Protein transport;Reference proteome;Transport;Ubl conjugation SUBCELLULAR LOCATION: Cytoplasm Preautophagosomal structure membrane ; Peripheral membrane protein " Q54GV7,"PROTEIN NAMES: NAD-dependent deacetylase sir2D (Silent information regulator sir2D) PROTEIN FAMILY: Sirtuin family ORGANISM: Dictyostelium discoideum (Social amoeba) SIMILARITY: Belongs to the sirtuin family. FUNCTION: NAD-dependent deacetylase, which plays an important role in the regulation of transcriptional repression. KEYWORDS: Coiled coil;Metal-binding;NAD;Reference proteome;Transferase;Zinc " Q54HH2,PROTEIN NAMES: Serine racemase (D-serine ammonia-lyase) (D-serine dehydratase) (L-serine ammonia-lyase) (L-serine dehydratase) PROTEIN FAMILY: Serine/threonine dehydratase family ORGANISM: Dictyostelium discoideum (Social amoeba) SIMILARITY: Belongs to the serine/threonine dehydratase family. FUNCTION: Catalyzes the synthesis of D-serine from L-serine (By similarity). Has dehydratase activity towards both L-serine and D-serine (By similarity). KEYWORDS: 3D-structure;Allosteric enzyme;ATP-binding;Calcium;Isomerase;Lyase;Magnesium;Manganese;Metal-binding;Nucleotide-binding;Pyridoxal phosphate;Reference proteome Q54HI5,"PROTEIN NAMES: Lamin-like protein (Protein NE81) PROTEIN FAMILY: Intermediate filament family ORGANISM: Dictyostelium discoideum (Social amoeba) SIMILARITY: Belongs to the intermediate filament family. FUNCTION: Lamins are intermediate filament proteins that assemble into a filamentous meshwork, and which constitute the major components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane. Lamins provide a framework for the nuclear envelope, bridging the nuclear envelope and chromatin, thereby playing an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics (By similarity). The structural integrity of the lamina is strictly controlled by the cell cycle, as seen by the disintegration and formation of the nuclear envelope in prophase and telophase, respectively (By similarity). Helps to maintain integrity of nuclear structures in response to mechanical stress. KEYWORDS: Coiled coil;Intermediate filament;Membrane;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus lamina Nucleus envelope Nucleus inner membrane " Q54JH4,"PROTEIN NAMES: P2X receptor E (P2XE) PROTEIN FAMILY: P2X receptor family ORGANISM: Dictyostelium discoideum (Social amoeba) SIMILARITY: Belongs to the P2X receptor family. FUNCTION: P2X receptors are ATP-gated ion channels that play a role in intracellular calcium signaling. Not required for the purinergic response to extracellular nucleotides. Not essential for osmoregulation. Inward currents evoked by intracellular ATP. ATP analog beta, gamma-imido-ATP is a weak partial agonist of p2xE. Exclusively selective for ATP over other nucleotides. Insensitive to copper and P2 receptor antagonists PPADS and suramin but strongly inhibited by sodium ions. More permeable to ammonium than either sodium or potassium ions and less permeable to choline. Permeable to calcium ions, but not chloride. KEYWORDS: Ion channel;Ion transport;Ligand-gated ion channel;Membrane;Receptor;Reference proteome;Transmembrane;Transmembrane helix;Transport;Vacuole SUBCELLULAR LOCATION: Contractile vacuole membrane Note=Ligand binding domain within the lumen of the vacuole. " Q54JH6,"PROTEIN NAMES: DNA (cytosine-5)-methyltransferase PROTEIN FAMILY: Class I-like SAM-binding methyltransferase superfamily, C5-methyltransferase family ORGANISM: Dictyostelium discoideum (Social amoeba) SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family. FUNCTION: Involved in epigenetic gene silencing. Methylates specific cytosine residues in the retrotransposons DIRS-1 and Skipper. KEYWORDS: Chromatin regulator;DNA-binding;Methyltransferase;Nucleus;Reference proteome;S-adenosyl-L-methionine;Transcription;Transcription regulation;Transferase SUBCELLULAR LOCATION: Nucleus " Q54JY7,PROTEIN NAMES: Syntaxin-7A PROTEIN FAMILY: Syntaxin family ORGANISM: Dictyostelium discoideum (Social amoeba) SIMILARITY: Belongs to the syntaxin family. FUNCTION: Involved in the targeting and/or fusion of transport vesicles to their target membrane during transport of proteins from the early endosome to the lysosome. Required for fusion of late endosomes with lysosomes and homotypic lysosomal fusion. May be involved in protein trafficking from the plasma membrane to the early endosome (EE) as well as in homotypic fusion of endocytic organelles. KEYWORDS: Coiled coil;Endosome;Membrane;Phosphoprotein;Reference proteome;Signal-anchor;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Endosome membrane ; Single-pass membrane protein Q54KQ7,"PROTEIN NAMES: Spastin PROTEIN FAMILY: AAA ATPase family, Spastin subfamily ORGANISM: Dictyostelium discoideum (Social amoeba) SIMILARITY: Belongs to the AAA ATPase family. Spastin subfamily. FUNCTION: ATP-dependent microtubule severing protein. Microtubule severing may promote reorganization of cellular microtubule arrays and the release of microtubules from the microtubule organizing center following nucleation. KEYWORDS: ATP-binding;Cytoplasm;Cytoskeleton;Isomerase;Membrane;Microtubule;Nucleotide-binding;Reference proteome SUBCELLULAR LOCATION: Membrane ; Peripheral membrane protein Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Cytoplasm, cytoskeleton Note=Forms an intramembrane hairpin-like structure in the membrane. " Q54ND5,"PROTEIN NAMES: Multiple inositol polyphosphate phosphatase 1 (Ddmipp1) (2,3-bisphosphoglycerate 3-phosphatase) (2,3-BPG phosphatase) PROTEIN FAMILY: Histidine acid phosphatase family, MINPP1 subfamily ORGANISM: Dictyostelium discoideum (Social amoeba) SIMILARITY: Belongs to the histidine acid phosphatase family. MINPP1 subfamily. FUNCTION: Probable multiple inositol polyphosphate phosphatase that hydrolyzes 1D-myo-inositol 1,3,4,5,6-pentakisphosphate (InsP5[2OH]) and 1D-myo-inositol hexakisphosphate (InsP6) to a range of less phosphorylated inositol phosphates. This regulates the availability of these various small molecule second messengers and metal chelators which control many aspects of cell physiology (By similarity). May have a dual substrate specificity, and function as a 2,3-bisphosphoglycerate 3-phosphatase hydrolyzing 2,3-bisphosphoglycerate to 2-phosphoglycerate. 2,3-bisphosphoglycerate (BPG) is formed as part of the Rapoport-Luebering glycolytic bypass. KEYWORDS: Hydrolase;Membrane;Reference proteome;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Single-pass type I membrane protein " Q54NL4,"PROTEIN NAMES: Vacuole membrane protein 1 homolog (Transmembrane protein 49 homolog) PROTEIN FAMILY: VMP1 family ORGANISM: Dictyostelium discoideum (Social amoeba) SIMILARITY: Belongs to the VMP1 family. FUNCTION: Phospholipid scramblase involved in lipid homeostasis and membrane dynamics processes (By similarity). Required for autophagosome formation: participates in early stages of autophagosome biogenesis at the endoplasmic reticulum (ER) membrane by reequilibrating the leaflets of the ER as lipids are extracted (By similarity). In addition to autophagy, involved in other processes in which phospholipid scramblase activity is required. KEYWORDS: Coiled coil;Endoplasmic reticulum;Lipid transport;Membrane;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Membrane ; Multi-pass membrane protein Endoplasmic reticulum " Q54QE6,"PROTEIN NAMES: NAD-dependent deacetylase sir2A (Silent information regulator sir2A) PROTEIN FAMILY: Sirtuin family ORGANISM: Dictyostelium discoideum (Social amoeba) SIMILARITY: Belongs to the sirtuin family. FUNCTION: NAD-dependent deacetylase, which plays an important role in the regulation of transcriptional repression. KEYWORDS: Metal-binding;NAD;Reference proteome;Transferase;Zinc;Zinc-finger " Q54RB7,"PROTEIN NAMES: Dual specificity protein kinase shkA (SH2 domain-containing protein 1) (SH2 domain-containing protein A) PROTEIN FAMILY: Protein kinase superfamily, TKL Ser/Thr protein kinase family, SH2 domain-containing protein kinase subfamily ORGANISM: Dictyostelium discoideum (Social amoeba) SIMILARITY: Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. SH2 domain-containing protein kinase subfamily. FUNCTION: Required for proper chemotaxis and phagocytosis; proper spatiotemporal control of F-actin levels in chemotaxing cells. Negative regulator of the PI3K (phosphatidylinositol 3 kinase) pathway. Predominantly phosphorylates serines and threonines and tyrosines at a lower level. KEYWORDS: ATP-binding;Kinase;Membrane;Nucleotide-binding;Reference proteome;Serine/threonine-protein kinase;Transferase;Tyrosine-protein kinase SUBCELLULAR LOCATION: Membrane " Q54RV9,"PROTEIN NAMES: Sphingosine-1-phosphate lyase (S1P lyase) (S1PL) (SP-lyase) (SPL) (Sphingosine-1-phosphate aldolase) PROTEIN FAMILY: Group II decarboxylase family, Sphingosine-1-phosphate lyase subfamily ORGANISM: Dictyostelium discoideum (Social amoeba) SIMILARITY: Belongs to the group II decarboxylase family. Sphingosine-1-phosphate lyase subfamily. FUNCTION: Cleaves phosphorylated sphingoid bases (PSBs), such as sphingosine-1-phosphate, into fatty aldehydes and phosphoethanolamine (By similarity). Sphingosine-1-phosphate (S1P) probably acts intracellularly as a second messenger perhaps by promoting cell proliferation; the absence of S1P lyase increases its concentration. This leads to increased lateral pseudopod formation as well as defects in the efficiency of chemotaxis. Overexpression of S1P lyase causes decreased growth rates, entry into stationary phase at lower cell density and increased sensitivity to the antitumor agents cisplatin and carboplatin; these effects are more pronounced in cells that express more enzyme. PATHWAY: Lipid metabolism; sphingolipid metabolism. KEYWORDS: Endoplasmic reticulum;Lipid metabolism;Lyase;Membrane;Pyridoxal phosphate;Reference proteome;Signal-anchor;Sphingolipid metabolism;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Single-pass membrane protein " Q54SS8,PROTEIN NAMES: RapA guanosine triphosphatase-activating protein B ORGANISM: Dictyostelium discoideum (Social amoeba) FUNCTION: Mediates the deactivation of rap1 during multicellular development and is required for normal morphogenesis. Also required for the correct patterning of specific subtypes of prestalk cells. KEYWORDS: GTPase activation;Reference proteome Q54U89,"PROTEIN NAMES: Metabotropic glutamate receptor-like protein L PROTEIN FAMILY: BMP lipoprotein family; G-protein coupled receptor 3 family, GABA-B receptor subfamily ORGANISM: Dictyostelium discoideum (Social amoeba) SIMILARITY: In the N-terminal section; belongs to the BMP lipoprotein family.; SIMILARITY: In the C-terminal section; belongs to the G-protein coupled receptor 3 family. GABA-B receptor subfamily. KEYWORDS: G-protein coupled receptor;Glycoprotein;Membrane;Receptor;Reference proteome;Signal;Transducer;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Multi-pass membrane protein " Q54VB6,"PROTEIN NAMES: Serine/threonine-protein phosphatase 2A regulatory subunit psrA (Protein PPP2R5A/B56 homolog) (Protein phosphatase 2A regulatory B subunit) (Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit) (protein phosphatase 2A B56 regulatory subunit homolog) PROTEIN FAMILY: Phosphatase 2A regulatory subunit B56 family ORGANISM: Dictyostelium discoideum (Social amoeba) SIMILARITY: Belongs to the phosphatase 2A regulatory subunit B56 family. FUNCTION: Involved in developmental cell fate decision. KEYWORDS: Cytoplasm;Reference proteome SUBCELLULAR LOCATION: Cytoplasm, cytosol " Q54W90,"PROTEIN NAMES: Protein SEY1 homolog PROTEIN FAMILY: TRAFAC class dynamin-like GTPase superfamily, GB1/RHD3 GTPase family, RHD3 subfamily ORGANISM: Dictyostelium discoideum (Social amoeba) SIMILARITY: Belongs to the TRAFAC class dynamin-like GTPase superfamily. GB1/RHD3 GTPase family. RHD3 subfamily. FUNCTION: Probable GTP-binding protein that may be involved in cell development. KEYWORDS: Coiled coil;Endoplasmic reticulum;GTP-binding;Hydrolase;Membrane;Nucleotide-binding;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein " Q54WT8,"PROTEIN NAMES: Aquaporin-B PROTEIN FAMILY: MIP/aquaporin family ORGANISM: Dictyostelium discoideum (Social amoeba) SIMILARITY: Belongs to the MIP/aquaporin (TC 1.A.8) family. FUNCTION: Putatively gated water-specific channel, requiring a cysteine residue within the channel. Impermeable to water, glycerol and urea when expressed in Xenopus oocytes. Not regulated by pH; channels remain impermeable to water at pH 7.4 and 5.2. KEYWORDS: Alternative initiation;Cell membrane;Cytoplasmic vesicle;Glycoprotein;Membrane;Phosphoprotein;Reference proteome;Repeat;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Cytoplasmic vesicle Note=Expressed in lamellipodia-like protrusions of the plasma membrane and intracellular vacuolar structures. " Q55080,"PROTEIN NAMES: Cytochrome P450 119 (Peroxidase) PROTEIN FAMILY: Cytochrome P450 family ORGANISM: Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770) SIMILARITY: Belongs to the cytochrome P450 family. FUNCTION: The endogenous substrate is not known. In vitro, catalyzes the H(2)O(2)-dependent epoxidation of styrene, cis-beta-methylstyrene, and cis-stilbene with retention of stereochemistry. Is able to use cumene hydroperoxide (CHP) or tert-butyl hydroperoxide (TBHP) instead of H(2)O(2) as the electron acceptor. Can also hydroxylate fatty acids such as lauric acid. KEYWORDS: 3D-structure;Cytoplasm;Heme;Iron;Metal-binding;Monooxygenase;Oxidoreductase;Peroxidase;Reference proteome SUBCELLULAR LOCATION: Cytoplasm " Q55215,"PROTEIN NAMES: Aklanonic acid methyl ester cyclase DauD (AAME cyclase) (Methyl aklanonate cyclase) PROTEIN FAMILY: Polyketide cyclase DnrD family ORGANISM: Streptomyces sp. (strain C5) SIMILARITY: Belongs to the polyketide cyclase DnrD family. FUNCTION: Involved in the biosynthesis of aklavinone which is an important precursor common to the formation of the clinically significant anthracyclines such as carminomycin, daunorubicin (daunomycin), rhodomycin, aclacinomycin T (aklavin) and aclacinomycin A (aclarubicin). These compounds are aromatic polyketide antibiotics that exhibit high cytotoxicity and are widely applied in the chemotherapy of a variety of cancers. Catalyzes the cyclization of aklanonic acid methyl ester to yield aklaviketone. PATHWAY: Antibiotic biosynthesis; daunorubicin biosynthesis.; PATHWAY: Antibiotic biosynthesis; carminomycin biosynthesis.; PATHWAY: Antibiotic biosynthesis; rhodomycin biosynthesis.; PATHWAY: Antibiotic biosynthesis; aclacinomycin biosynthesis. KEYWORDS: Antibiotic biosynthesis;Isomerase " Q552S0,"PROTEIN NAMES: Sodium/hydrogen exchanger 1 (DdNHE1) (NHE1) (Na-H exchanger 1) PROTEIN FAMILY: Monovalent cation:proton antiporter 1 (CPA1) transporter family ORGANISM: Dictyostelium discoideum (Social amoeba) SIMILARITY: Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family. FUNCTION: Regulation of intracellular pH homeostasis in response to cAMP, which is essential for chemotaxis. Necessary for F-actin localization and the kinetics of actin polymerization during chemotaxis and cell polarity but not for directional sensing. KEYWORDS: Antiport;Ion transport;Membrane;Reference proteome;Signal;Sodium;Sodium transport;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Membrane ; Multi-pass membrane protein Note=Predominantly localizes to the leading-edge of the chemotaxing polarized cells. " Q55650,"PROTEIN NAMES: (S)-8-amino-7-oxononanoate synthase BioU (8-amino-7-oxononanoate carboxylating dehydrogenase) PROTEIN FAMILY: BioU family ORGANISM: Synechocystis sp. (strain PCC 6803 / Kazusa) SIMILARITY: Belongs to the BioU family. FUNCTION: A 'suicide' enzyme that participates in biotin synthesis. Catalyzes the formation of (S)-8-amino-7-oxononanoate (DAN-carbamic acid) from (7R,8S)-8-amino-7-(carboxyamino)nonanoate (DAN), a function equivalent to the cannonical BioA reaction and the first half-reaction of BioD. The cellular requirement for biotin is thought be low enough that this single turnover enzyme supplies a sufficient amount of the cofactor. Overall it catalyzes three reactions: formation of a covalent linkage with 8-amino-7-oxononanoate to yield a BioU-DAN conjugate at the epsilon-amino group of Lys124 of BioU using NAD(P)H, carboxylation of the conjugate to form BioU-DAN-carbamic acid, and release of DAN-carbamic acid using NAD(P)+ (By similarity). A coupled Synechocystis BioU/BioD assay produces dethiobiotin from DAN. Complements a bioA deletion in E.coli but not a bioD1 deletion. PATHWAY: Cofactor biosynthesis; biotin biosynthesis. KEYWORDS: 3D-structure;Aminotransferase;Biotin biosynthesis;Direct protein sequencing;NAD;NADP;Nucleotide-binding;Reference proteome;Transferase MISCELLANEOUS: In canonical biotin synthesis a pimeloyl-conjugate is transformed into biotin by the subsequent action of BioF, BioA, BioD and BioB. This enzyme replaces BioA and performs the first half-reaction of BioD. In Synechocystis BioD acts on the product of this enzyme." Q556N5,PROTEIN NAMES: Proteasomal ubiquitin receptor ADRM1 homolog PROTEIN FAMILY: ADRM1 family ORGANISM: Dictyostelium discoideum (Social amoeba) SIMILARITY: Belongs to the ADRM1 family. FUNCTION: Functions as a proteasomal ubiquitin receptor. Recruits the deubiquitinating enzyme uchl5 at the 26S proteasome and promotes its activity (By similarity). Plays a role in the transition from growth to differentiation. KEYWORDS: Cytoplasm;Nucleus;Phosphoprotein;Proteasome;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Nucleus Q556W1,PROTEIN NAMES: D-amino-acid oxidase (DAAO) (DAMOX) (DAO) PROTEIN FAMILY: DAMOX/DASOX family ORGANISM: Dictyostelium discoideum (Social amoeba) SIMILARITY: Belongs to the DAMOX/DASOX family. FUNCTION: Catalyzes the oxidative deamination of D-amino acids with broad substrate specificity. Has low in vitro and no in vivo activity on D-serine; primary D-serine degradation is performed by the D-serine dehydratase dsd. KEYWORDS: FAD;Flavoprotein;Oxidoreductase;Peroxisome;Reference proteome SUBCELLULAR LOCATION: Peroxisome matrix Q55804,"PROTEIN NAMES: RNA helicase CrhR PROTEIN FAMILY: DEAD box helicase family ORGANISM: Synechocystis sp. (strain PCC 6803 / Kazusa) SIMILARITY: Belongs to the DEAD box helicase family. FUNCTION: An ATP-dependent bidirectional RNA helicase with RNA-dependent ATPase activity; does not unwind dsDNA, uses only (d)ATP. Also has ATP-dependent RNA annealing activity; concurrent annealing and helicase activity promote strand-exchange activity. In vitro has low helicase processivity, annealing processivity is probably higher. Required for correct cold adaptation, probably by aiding translation of mRNAs required for photosynthesis and electron transport. Probably regulates the cold-shock-inducible expression of the GroESL chaperones. May partially regulate its own expression at both the transcriptional and post-transcriptional level (experiments used a construct expressing a 25 kDa trunacted protein which might have dominant-negative effects); is probably not directly involved in the pathway responsible for mRNA degradation. KEYWORDS: ATP-binding;Cell inner membrane;Cell membrane;Cytoplasm;Helicase;Hydrolase;Membrane;Nucleotide-binding;Reference proteome;Stress response;Thylakoid SUBCELLULAR LOCATION: Cytoplasm Cell inner membrane ; Peripheral membrane protein Cellular thylakoid membrane ; Peripheral membrane protein Note=A small amount is localized to the cell inner membrane and the thylakoid membrane, and cosediments with polysomes. " Q558Z2,"PROTEIN NAMES: Sun domain-containing protein 1 ORGANISM: Dictyostelium discoideum (Social amoeba) FUNCTION: May have an important role in defining the spacing of the nuclear envelope lumen. Essential for centrosome attachment to the nucleus, maintenance of correct ploidy, proper mitosis, association of the centromere cluster with the centrosome and the maintenance of genome stability. Requires direct chromatin binding for inner nuclear membrane targeting. KEYWORDS: Cell cycle;Cell division;Coiled coil;Membrane;Mitosis;Nucleus;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Nucleus membrane ; Single-pass membrane protein ; Nucleoplasmic side " Q55BN8,"PROTEIN NAMES: Probable serine/threonine-protein kinase nek2 (Never in mitosis protein A-related protein kinase 2) (NimA-related protein kinase 2) PROTEIN FAMILY: Protein kinase superfamily, NEK Ser/Thr protein kinase family, NIMA subfamily ORGANISM: Dictyostelium discoideum (Social amoeba) SIMILARITY: Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily. FUNCTION: Involved in centrosome biogenesis. Seems to be required for recruitment of centrosomal material and might be involved in de novo centrosome formation. KEYWORDS: ATP-binding;Coiled coil;Kinase;Nucleotide-binding;Reference proteome;Serine/threonine-protein kinase;Transferase " Q55BW0,"PROTEIN NAMES: Ras-related protein rapC PROTEIN FAMILY: Small GTPase superfamily, Ras family ORGANISM: Dictyostelium discoideum (Social amoeba) SIMILARITY: Belongs to the small GTPase superfamily. Ras family. KEYWORDS: Cell membrane;GTP-binding;Hydrolase;Lipoprotein;Membrane;Methylation;Nucleotide-binding;Prenylation;Reference proteome SUBCELLULAR LOCATION: Cell membrane ; Lipid-anchor ; Cytoplasmic side " Q55C57,"PROTEIN NAMES: Probable serine/threonine-protein kinase glkA (Glycogen synthase kinase-like kinase A) PROTEIN FAMILY: Protein kinase superfamily, CMGC Ser/Thr protein kinase family, GSK-3 subfamily ORGANISM: Dictyostelium discoideum (Social amoeba) SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily. KEYWORDS: ATP-binding;Kinase;Nucleotide-binding;Reference proteome;Serine/threonine-protein kinase;Transferase " Q55CK2,PROTEIN NAMES: GTPase activating protein homolog 2 (GTPase activating factor for raC protein CC) (Rho GTPase-activating protein gacCC) ORGANISM: Dictyostelium discoideum (Social amoeba) FUNCTION: Rho GTPase-activating protein involved in the signal transduction pathway (By similarity). Regulator of the contractile vacuole network as well as involved in driving vacuole emptying. KEYWORDS: Coiled coil;Cytoplasm;GTPase activation;Reference proteome;Vacuole SUBCELLULAR LOCATION: Cytoplasm. Contractile vacuole Q55G45,PROTEIN NAMES: Probable mitochondrial Rho GTPase gemA (Miro) PROTEIN FAMILY: Mitochondrial Rho GTPase family ORGANISM: Dictyostelium discoideum (Social amoeba) SIMILARITY: Belongs to the mitochondrial Rho GTPase family. FUNCTION: Mitochondrial GTPase involved in mitochondrial trafficking. Probably involved in control of anterograde transport of mitochondria and their subcellular distribution (By similarity). KEYWORDS: Calcium;GTP-binding;Hydrolase;Membrane;Metal-binding;Mitochondrion;Mitochondrion outer membrane;Nucleotide-binding;Reference proteome;Repeat;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Mitochondrion outer membrane ; Single-pass type IV membrane protein Q55GK8,PROTEIN NAMES: Membrane-associated sulfotransferase kil1 PROTEIN FAMILY: Sulfotransferase 1 family ORGANISM: Dictyostelium discoideum (Social amoeba) SIMILARITY: Belongs to the sulfotransferase 1 family. FUNCTION: Sulfotransferase involved in intracellular killing of bacteria. KEYWORDS: Glycoprotein;Membrane;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Single-pass type II membrane protein Q56837,"PROTEIN NAMES: 2-hydroxypropyl-CoM lyase (Aliphatic epoxide carboxylation component I) (Epoxide carboxylase component I) (Epoxyalkane:CoM transferase) (EaCoMT) PROTEIN FAMILY: Vitamin-B12 independent methionine synthase family ORGANISM: Xanthobacter autotrophicus (strain ATCC BAA-1158 / Py2) SIMILARITY: Belongs to the vitamin-B12 independent methionine synthase family. FUNCTION: Involved in aliphatic epoxide carboxylation. Catalyzes the addition of coenzyme M (CoM) to either R- or S-epoxypropane to form the thioether conjugate 2-hydroxypropyl-CoM. Catalyzes the reaction of CoM with R-epoxypropane at a rate approximately twice of that with S-epoxypropane. The CoM analogs 2-mercaptopropionate, 2-mercaptoethanol and cysteine substitute poorly for CoM as the thiol substrate. PATHWAY: Alkene metabolism; propylene degradation. KEYWORDS: Direct protein sequencing;Lyase;Metal-binding;Plasmid;Reference proteome;Zinc " Q56840,PROTEIN NAMES: 2-(R)-hydroxypropyl-CoM dehydrogenase (R-HPCDH) (2-[(R)-2-hydroxypropylthio]ethanesulfonate dehydrogenase) (Aliphatic epoxide carboxylation component III) (Epoxide carboxylase component III) (RHPCDH1) PROTEIN FAMILY: Short-chain dehydrogenases/reductases (SDR) family ORGANISM: Xanthobacter autotrophicus (strain ATCC BAA-1158 / Py2) SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FUNCTION: Involved in aliphatic epoxide carboxylation. Catalyzes the reversible oxidation of (R)-2-hydroxypropyl-coenzyme M (R-HPC) to 2-oxopropyl-coenzyme M (2-KPC). The enzyme is highly specific for the R enantiomers. In vitro can also use achiral 2-propanol and short-chain (R)- and (S)-2-alkanols. PATHWAY: Alkene metabolism; propylene degradation. KEYWORDS: 3D-structure;Direct protein sequencing;NAD;Oxidoreductase;Plasmid;Reference proteome MISCELLANEOUS: Enantioselectivity is dictated largely by differences in kcat. Q568P9,"PROTEIN NAMES: Small RNA 2'-O-methyltransferase (HEN1 methyltransferase homolog 1) PROTEIN FAMILY: Methyltransferase superfamily, HEN1 family ORGANISM: Danio rerio (Zebrafish) (Brachydanio rerio) SIMILARITY: Belongs to the methyltransferase superfamily. HEN1 family. FUNCTION: Methyltransferase that adds a 2'-O-methyl group at the 3'-end of piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. This probably protects the 3'-end of piRNAs from uridylation and adenylation activities and subsequent degradation. Stabilization of piRNAs is essential for oocyte development. KEYWORDS: Cytoplasm;Differentiation;Magnesium;Metal-binding;Methyltransferase;Oogenesis;Reference proteome;RNA-binding;RNA-mediated gene silencing;S-adenosyl-L-methionine;Transferase SUBCELLULAR LOCATION: Cytoplasm Note=Component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon activity during meiosis. " Q56NI9,"PROTEIN NAMES: N-acetyltransferase ESCO2 (Establishment factor-like protein 2) (EFO2) (EFO2p) (hEFO2) (Establishment of cohesion 1 homolog 2) PROTEIN FAMILY: Acetyltransferase family, ECO subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the acetyltransferase family. ECO subfamily. FUNCTION: Acetyltransferase required for the establishment of sister chromatid cohesion. Couples the processes of cohesion and DNA replication to ensure that only sister chromatids become paired together. In contrast to the structural cohesins, the deposition and establishment factors are required only during the S phase. Acetylates the cohesin component SMC3. KEYWORDS: Acyltransferase;Alternative splicing;Cell cycle;Chromosome;Disease variant;Dwarfism;Intellectual disability;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Transferase;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Chromosome Note=Nuclear in interphase cells, excluded from chromosomes during metaphase but reassociates with chromosomes in telophase. " Q56XM9,PROTEIN NAMES: Nuclear poly(A) polymerase 3 (PAP(III)) (Poly(A) polymerase III) (Polynucleotide adenylyltransferase 3) PROTEIN FAMILY: Poly(A) polymerase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the poly(A) polymerase family. FUNCTION: Essential protein. Polymerase that creates the 3'-poly(A) tail of mRNA's. Also required for the endoribonucleolytic cleavage reaction at some polyadenylation sites. May acquire specificity through interaction with a cleavage and polyadenylation specificity factor (CPSF) at its C-terminus (By similarity). KEYWORDS: Alternative splicing;ATP-binding;Magnesium;Metal-binding;mRNA processing;Nucleotide-binding;Nucleotidyltransferase;Nucleus;Reference proteome;Transferase SUBCELLULAR LOCATION: Nucleus Q56Y52,"PROTEIN NAMES: Protection of telomeres protein 1a (AtPOT1a) (AtPot1) (Protection of telomeres protein 1) PROTEIN FAMILY: Telombin family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the telombin family. FUNCTION: Component of the telomerase ribonucleoprotein (RNP) complex that is essential for the positive regulation of telomere length. Binds RNA non-specifically. Binds specifically single-stranded telomeric DNA. Not required to recruit telomerase to telomeres, but stimulates TER1 RNP repeat addition processivity. KEYWORDS: Alternative splicing;Chromosome;Cytoplasm;DNA-binding;Nucleus;Reference proteome;RNA-binding;Telomere SUBCELLULAR LOCATION: Nucleus Chromosome, telomere Nucleus, nucleolus Cytoplasm Note=Localizes to telomeres in S-phase. Localizes to cytoplasmic foci. " Q57573,"PROTEIN NAMES: Fructose-1,6-bisphosphatase/inositol-1-monophosphatase (FBPase/IMPase) (Inositol-1-phosphatase) (I-1-Pase) PROTEIN FAMILY: Inositol monophosphatase superfamily, FBPase class 4 family ORGANISM: Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) SIMILARITY: Belongs to the inositol monophosphatase superfamily. FBPase class 4 family. FUNCTION: Phosphatase with broad specificity; it can dephosphorylate fructose 1,6-bisphosphate, both D and L isomers of inositol-1-phosphate (I-1-P), 2'-AMP, pNPP, beta-glycerol phosphate, and alpha-D-glucose-1-phosphate. Cannot hydrolyze glucose-6-phosphate, fructose-6-phosphate, NAD(+) or 5'-AMP. May be involved in the biosynthesis of a unique osmolyte, di-myo-inositol 1,1-phosphate. KEYWORDS: 3D-structure;Carbohydrate metabolism;Hydrolase;Magnesium;Metal-binding;Reference proteome " Q57688,"PROTEIN NAMES: Bifunctional thiamine biosynthesis protein ThiDN [Includes: Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase (Hydroxymethylpyrimidine kinase) (HMP kinase) (Hydroxymethylpyrimidine phosphate kinase) (HMP-P kinase) (HMP-phosphate kinase) (HMPP kinase); Thiamine-phosphate synthase ThiN (TP synthase) (TPS) (Thiamine-phosphate pyrophosphorylase) (TMP pyrophosphorylase) (TMP-PPase)] PROTEIN FAMILY: ThiD family; ThiN family ORGANISM: Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) SIMILARITY: In the N-terminal section; belongs to the ThiD family.; SIMILARITY: In the C-terminal section; belongs to the ThiN family. FUNCTION: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P.; FUNCTION: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP). PATHWAY: Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-amino-2-methyl-5-diphosphomethylpyrimidine from 5-amino-1-(5-phospho-D-ribosyl)imidazole.; PATHWAY: Cofactor biosynthesis; thiamine diphosphate biosynthesis; thiamine phosphate from 4-amino-2-methyl-5-diphosphomethylpyrimidine and 4-methyl-5-(2-phosphoethyl)-thiazole: step 1/1. KEYWORDS: 3D-structure;ATP-binding;Kinase;Multifunctional enzyme;Nucleotide-binding;Reference proteome;Thiamine biosynthesis;Transferase " Q57849,"PROTEIN NAMES: Nucleoside kinase (NK) (ATP-dependent nucleoside monophosphokinase) (Cytidine kinase) (Guanosine-inosine kinase) PROTEIN FAMILY: Carbohydrate kinase PfkB family ORGANISM: Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) SIMILARITY: Belongs to the carbohydrate kinase PfkB family. FUNCTION: Catalyzes the phosphorylation of a wide range of nucleosides to yield nucleoside monophosphates. Shows the highest activity for inosine, guanosine and cytidine, but very poor kinase activity with adenosine, thymidine, uridine and xanthosine. ATP is the best phosphate donor, but can also use ITP and GTP. Shows extremely low activity with fructose-6-phosphate. KEYWORDS: 3D-structure;ATP-binding;Direct protein sequencing;Kinase;Magnesium;Manganese;Nucleotide-binding;Reference proteome;Transferase " Q57977,"PROTEIN NAMES: Ribosomal RNA small subunit methyltransferase Nep1 (16S rRNA (pseudouridine-N1-)-methyltransferase Nep1) (16S rRNA Psi914 methyltransferase) PROTEIN FAMILY: Class IV-like SAM-binding methyltransferase superfamily, RNA methyltransferase NEP1 family ORGANISM: Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) SIMILARITY: Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase NEP1 family. FUNCTION: Methyltransferase involved in ribosomal biogenesis. Specifically catalyzes the N1-methylation of pseudouridine at position 914 (Psi914) in 16S rRNA. Is not able to methylate uridine at this position. KEYWORDS: 3D-structure;Methyltransferase;Reference proteome;Ribosome biogenesis;RNA-binding;rRNA processing;rRNA-binding;S-adenosyl-L-methionine;Transferase " Q57UZ7,"PROTEIN NAMES: PAB1-binding protein 1 (Ataxin-2 homolog) (Poly(A)-binding protein-binding protein) PROTEIN FAMILY: Ataxin-2 family ORGANISM: Trypanosoma brucei brucei (strain 927/4 GUTat10.1) SIMILARITY: Belongs to the ataxin-2 family. FUNCTION: Involved in post-transcriptional regulation of gene expression. Promotes mRNA stabilization by bridging poly(A)-binding protein to mRNAs. KEYWORDS: Cytoplasm;Reference proteome;Translation regulation SUBCELLULAR LOCATION: Cytoplasm, cytosol Cytoplasm, Stress granule Note=Localizes to polysomes. Localizes to starvation-induced stress granules but not heat shock-induced stress granules. " Q57XK8,"PROTEIN NAMES: RNA-binding protein ZC3H20 (CCCH zinc finger protein ZC3H20) ORGANISM: Trypanosoma brucei brucei (strain 927/4 GUTat10.1) FUNCTION: RNA-binding protein involved in regulation of mRNA stability. Promotes mRNA stabilization by recruiting MKT1 and PBP1 (By similarity). Stabilizes mRNA required for differentiation from the bloodstream form to the procyclic form, and maintenance of the procyclic form. Stabilizes transcripts encoding membrane and mitochondrial proteins, including AQP1 and GPEET2 procyclin. KEYWORDS: Metal-binding;Reference proteome;Repeat;RNA-binding;Translation regulation;Zinc;Zinc-finger " Q582G4,"PROTEIN NAMES: Protein arginine N-methyltransferase 7 (TbPRMT7) PROTEIN FAMILY: Class I-like SAM-binding methyltransferase superfamily, Protein arginine N-methyltransferase family, PRMT7 subfamily ORGANISM: Trypanosoma brucei brucei (strain 927/4 GUTat10.1) SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N-methyltransferase family. PRMT7 subfamily. FUNCTION: Arginine methyltransferase that specifically catalyzes the formation of omega-N monomethylarginine (MMA). Has activity toward multiple substrates in vitro. Able to mediate the arginine methylation of histones and myelin basic protein (MBP) in vitro; the relevance of such results is however unclear in vivo. KEYWORDS: 3D-structure;Cytoplasm;Methyltransferase;Reference proteome;S-adenosyl-L-methionine;Transferase SUBCELLULAR LOCATION: Cytoplasm " Q58327,"PROTEIN NAMES: Bifunctional NADP phosphatase/NAD kinase [Includes: NAD kinase (ATP-dependent NAD kinase) (Poly(P)-dependent NAD kinase) (PPNK); NADP phosphatase (NADPase) (pNPPase) ] PROTEIN FAMILY: Inositol monophosphatase superfamily; NAD kinase family ORGANISM: Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) SIMILARITY: In the N-terminal section; belongs to the inositol monophosphatase superfamily.; SIMILARITY: In the C-terminal section; belongs to the NAD kinase family. FUNCTION: Involved in the regulation of the intracellular balance between NAD(H) and NADP(H), and is a key enzyme in the biosynthesis of NADP. Catalyzes the phosphorylation and dephosphorylation of NAD and NADP, respectively. Although it shows conflicting dual activities and is able to supply NADP, it seems that its physiological role is to prevent excess accumulation of NADP. Kinase can use ATP and other nucleoside triphosphates (UTP, TTP, CTP, GTP) as well as inorganic polyphosphate (poly(P)) as phosphoryl donors, however poly(P) is not considered to be the physiological phosphoryl donor. NAD is the preferred substrate for the kinase, but NADH can also be used as phosphoryl acceptor. Phosphatase can use NADP or NADPH as phosphoryl donor, but NADP is the preferred substrate. Phosphatase also has an activity toward the terminal phosphate group at C-2 of adenosine in 2'-AMP and toward the phosphate group at C-1 of fructose 1,6-bisphosphate, but not toward inositol 1-phosphate. KEYWORDS: ATP-binding;Cytoplasm;Direct protein sequencing;Hydrolase;Kinase;Magnesium;Metal-binding;Multifunctional enzyme;NAD;NADP;Nucleotide-binding;Reference proteome;Transferase SUBCELLULAR LOCATION: Cytoplasm MISCELLANEOUS: The phosphatase is inert toward the substrates of NAD kinase (NAD, NADH, ATP, and poly(P)). This demonstrates that the phosphatase activity never interferes with the NAD kinase activity by degrading its substrates." Q58407,"PROTEIN NAMES: Coenzyme gamma-F420-2:alpha-L-glutamate ligase PROTEIN FAMILY: RimK family, CofF subfamily ORGANISM: Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) SIMILARITY: Belongs to the RimK family. CofF subfamily. FUNCTION: Catalyzes the ATP-dependent addition of one alpha-linked L-glutamate molecule to coenzyme gamma-F420-2, producing alpha-F420-3, the major form of coenzyme F420 found in M.jannaschii. Thus, caps the gamma-polyglutamate tail of coenzyme F420 with a terminal alpha-linked glutamate. Prefers ATP to other purine nucleotide triphosphates; GTP gives about 25% of the activity observed with ATP. Cannot catalyze the addition of the following amino acids or analogs: D-glutamate, beta-glutamate, L-aspartate, L-glutamine, L-alpha-aminoadipate, or D,L-2-amino-4-phosphono-butyrate. PATHWAY: Cofactor biosynthesis; coenzyme F420 biosynthesis. KEYWORDS: ATP-binding;Ligase;Manganese;Metal-binding;Nucleotide-binding;Reference proteome " Q58487,"PROTEIN NAMES: Mevalonate kinase (MK) (MVK) PROTEIN FAMILY: GHMP kinase family, Mevalonate kinase subfamily ORGANISM: Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) SIMILARITY: Belongs to the GHMP kinase family. Mevalonate kinase subfamily. FUNCTION: Catalyzes the phosphorylation of (R)-mevalonate (MVA) to (R)-mevalonate 5-phosphate (MVAP). Functions in the mevalonate (MVA) pathway leading to isopentenyl diphosphate (IPP), a key precursor for the biosynthesis of isoprenoid compounds such as archaeal membrane lipids. PATHWAY: Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via mevalonate pathway; isopentenyl diphosphate from (R)-mevalonate: step 1/3. KEYWORDS: 3D-structure;ATP-binding;Cytoplasm;Isoprene biosynthesis;Kinase;Lipid biosynthesis;Lipid metabolism;Magnesium;Nucleotide-binding;Reference proteome;Transferase SUBCELLULAR LOCATION: Cytoplasm " Q58717,"PROTEIN NAMES: Protein argonaute (MjAgo) PROTEIN FAMILY: Argonaute family, Long pAgo subfamily ORGANISM: Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) SIMILARITY: Belongs to the argonaute family. Long pAgo subfamily. FUNCTION: A DNA-guided ssDNA endonuclease that may play a role in defense against invading genetic elements. Uses short ssDNA sequences as guides (gDNA) to bind complementary target strands, resulting in slicing of the target DNA (tDNA). Endonucleolytically cleaves tDNA (the gDNA indicates where to cleave); two major and two minor products are seen which correspond to cleavage sites between nucleotides 9/10, 10/11, 13/14, and 14/15 downstream of the target residue base-paired with the 5'-end of the gDNA. Efficient guide-dependent tDNA cleavage requires a minimal length of 15 bp and is maximal at 19 bp. Prefers gDNA with 5'-phosphorylated purines and 3'-pyrimidines; changing these bases alters the cleavage activity and patterns. Also has guide-independent activity on tDNA called 'chopping'. Probably a first round of guide-independent activity on an invading plasmid or virus would generate guide DNAs for subsequent, more efficient, guide-dependent degradation of invading nucleic acids. Has no activity on substrate with a mismatch at positions 10 and 11, on ssDNA or RNA, nor on DNA:RNA hybrids. Digests longer (750 bp) dsDNA as well as circular plasmid and naked genomic DNA, but not chromatin, in a guide DNA-independent manner. Addition of endogenous histone A3 protects DNA from cleavage, while cleavage is insensitive to methylation. When plasmid encoding active or mutated protein (Ala-541) is transformed into Sulfolobus acidocaldarius about 25-fold fewer transformants are found with active protein; reduced levels of plasmid are found in wild-type transformed cells. While S.acidocaldarius grows at a similar temperature to M.jannaschii (70 to 80 degrees Celsius) it has very different histone-like proteins, which presumably do not protect against MjAgo. Binds ssDNA, dsDNA and DNA-RNA hybrids; binding is most efficient with dsDNA. KEYWORDS: 3D-structure;Antiviral defense;DNA-binding;Endonuclease;Hydrolase;Manganese;Metal-binding;Nuclease;Reference proteome " Q58991,"PROTEIN NAMES: Homoisocitrate dehydrogenase (HICDH) (Homo(2)-isocitrate/homo(3)-isocitrate dehydrogenase) (Isohomocitrate dehydrogenase) (IHDH) (NAD-dependent threo-isohomocitrate dehydrogenase) PROTEIN FAMILY: Isocitrate and isopropylmalate dehydrogenases family ORGANISM: Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) SIMILARITY: Belongs to the isocitrate and isopropylmalate dehydrogenases family. FUNCTION: Catalyzes the NAD-dependent oxidation and decarboxylation of (2R,3S)-homoisocitrate, (2R,3S)-homo(2)-isocitrate and (2R,3S)-homo(3)-isocitrate, into 2-oxoadipate, 2-oxopimelate (2-oxoheptanedioate), and 2-oxosuberate, respectively. All these substrates are intermediates in the biosynthesis of biotin and of 7-mercaptoheptanoate, a moiety of coenzyme B in methanoarchaea. Is also able to produce 2-oxoazelate from (2R,3S)-homo(4)-isocitrate in vitro, but this substrate is probably not physiologically relevant. Is unable to use any isomer of isocitrate or isopropylmalate as a substrate, and NADP as an oxidant. PATHWAY: Organic acid metabolism; 2-oxosuberate biosynthesis. KEYWORDS: Magnesium;Metal-binding;NAD;Oxidoreductase;Phosphoprotein;Reference proteome " Q58999,"PROTEIN NAMES: Bifunctional ADP-specific glucokinase/phosphofructokinase (ADP-GK) (ADP-GK/PFK) (ADP-Pfk) (ADP-dependent glucokinase) (ADP-dependent phosphofructokinase) PROTEIN FAMILY: Carbohydrate kinase PfkC family ORGANISM: Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) SIMILARITY: Belongs to the carbohydrate kinase PfkC family. FUNCTION: Catalyzes the phosphorylation of fructose 6-phosphate and D-glucose to fructose 1,6-bisphosphate and D-glucose 6-phosphate, respectively, using ADP as the phosphate donor. PATHWAY: Carbohydrate degradation; glycolysis. KEYWORDS: 3D-structure;Cytoplasm;Glycolysis;Kinase;Magnesium;Metal-binding;Multifunctional enzyme;Reference proteome;Transferase SUBCELLULAR LOCATION: Cytoplasm. " Q589Y0,"PROTEIN NAMES: Phenolic glucoside malonyltransferase 1 (NtMaT1) (Flavonol/naphthol glucoside malonyltransferase 1) (Phenolic glucoside-6'-O-malonyltransferase 1) PROTEIN FAMILY: Plant acyltransferase family, Phenolic glucoside malonyltransferase subfamily ORGANISM: Nicotiana tabacum (Common tobacco) SIMILARITY: Belongs to the plant acyltransferase family. Phenolic glucoside malonyltransferase subfamily. FUNCTION: Malonyltransferase with broad substrate specificity acting on phenolic glucosides including xenobiotic naphthols. Has activity against flavonoid 7-O-glucosides, flavonoid 3-O-glucosides and naphthol glucosides, and to a lesser extent against coumarin glucosides in vitro. Prefers malonyl-CoA as an acyl donor, but also active with succinyl-CoA and methylmalonyl-CoA, but not with acetyl-CoA. KEYWORDS: 3D-structure;Acyltransferase;Detoxification;Reference proteome;Transferase " Q58DM4,"PROTEIN NAMES: DNA oxidative demethylase ALKBH2 (Alkylated DNA repair protein alkB homolog 2) (Alpha-ketoglutarate-dependent dioxygenase alkB homolog 2) PROTEIN FAMILY: AlkB family ORGANISM: Bos taurus (Bovine) SIMILARITY: Belongs to the alkB family. FUNCTION: Dioxygenase that repairs alkylated nucleic acid bases by direct reversal oxidative dealkylation. Can process both double-stranded (ds) and single-stranded (ss) DNA substrates, with a strong preference for dsDNA (By similarity). Uses molecular oxygen, 2-oxoglutarate and iron as cofactors to oxidize the alkyl groups that are subsequently released as aldehydes, regenerating the undamaged bases. Probes the base pair stability, locates a weakened base pair and flips the damaged base to accommodate the lesion in its active site for efficient catalysis (By similarity). Repairs monoalkylated bases, specifically N1-methyladenine and N3-methylcytosine, as well as higher order alkyl adducts such as bases modified with exocyclic bridged adducts known as etheno adducts including 1,N6-ethenoadenine, 3,N4-ethenocytosine and 1,N2-ethenoguanine (By similarity). Acts as a gatekeeper of genomic integrity under alkylation stress. Efficiently repairs alkylated lesions in ribosomal DNA (rDNA). These lesions can cause ss- and dsDNA strand breaks that severely impair rDNA transcription (By similarity). In a response mechanism to DNA damage, associates with PCNA at replication forks to repair alkylated adducts prior to replication (By similarity). KEYWORDS: Dioxygenase;DNA damage;DNA repair;Iron;Magnesium;Metal-binding;Nucleus;Oxidoreductase;Reference proteome SUBCELLULAR LOCATION: Nucleus Nucleus, nucleolus Nucleus, nucleoplasm Note=Relocates to the replication foci during S-phase. " Q58DX5,"PROTEIN NAMES: Inactive N-acetylated-alpha-linked acidic dipeptidase-like protein 2 (NAALADase L2) PROTEIN FAMILY: Peptidase M28 family, M28B subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the peptidase M28 family. M28B subfamily. FUNCTION: May be catalytically inactive. KEYWORDS: Alternative splicing;Glycoprotein;Membrane;Phosphoprotein;Reference proteome;Signal-anchor;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Single-pass type II membrane protein MISCELLANEOUS: The gene maps to 3q26.31, a region associated with Cornelia de Lange syndrome. However, previous research failed to identify specific mutations in a panel of DNA samples from patients with Cornelia de Lange syndrome." Q58NB6,PROTEIN NAMES: Dehydrogenase/reductase SDR family member 9 (3-alpha hydroxysteroid dehydrogenase) (3-alpha-HSD) (Retinol dehydrogenase) (Short-chain dehydrogenase/reductase retSDR8) PROTEIN FAMILY: Short-chain dehydrogenases/reductases (SDR) family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FUNCTION: 3-alpha-hydroxysteroid dehydrogenase that converts 3-alpha-tetrahydroprogesterone (allopregnanolone) to dihydroxyprogesterone and 3-alpha-androstanediol to dihydroxyprogesterone. Also plays a role in the biosynthesis of retinoic acid. Can utilize both NADH and NADPH. KEYWORDS: Endoplasmic reticulum;Lipid metabolism;Membrane;Microsome;NAD;NADP;Oxidoreductase;Reference proteome;Signal;Steroid metabolism SUBCELLULAR LOCATION: Microsome membrane Endoplasmic reticulum membrane Q59000,PROTEIN NAMES: Glucose-6-phosphate isomerase (GPI) (Phosphoglucose isomerase) (PGI) (Phosphohexose isomerase) (PHI) PROTEIN FAMILY: GPI family ORGANISM: Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) SIMILARITY: Belongs to the GPI family. FUNCTION: Catalyzes the isomerization of glucose-6-P to fructose-6-P. PATHWAY: Carbohydrate biosynthesis; gluconeogenesis.; PATHWAY: Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 2/4. KEYWORDS: Cytoplasm;Gluconeogenesis;Glycolysis;Isomerase;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Q59288,PROTEIN NAMES: Chondroitinase-AC (Chondroitin sulfate AC lyase) (Chondroitin-AC eliminase) (Chondroitin-AC lyase) PROTEIN FAMILY: Polysaccharide lyase 8 family ORGANISM: Pedobacter heparinus (strain ATCC 13125 / DSM 2366 / CIP 104194 / JCM 7457 / NBRC 12017 / NCIMB 9290 / NRRL B-14731 / HIM 762-3) SIMILARITY: Belongs to the polysaccharide lyase 8 family. KEYWORDS: 3D-structure;Calcium;Glycoprotein;Lyase;Metal-binding;Reference proteome;Signal Q59292,"PROTEIN NAMES: Probable multifunctional siroheme biosynthesis protein HemA [Includes: Glutamyl-tRNA reductase (GluTR) ; Precorrin-2 dehydrogenase ; Sirohydrochlorin ferrochelatase ] PROTEIN FAMILY: Glutamyl-tRNA reductase family; Precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase family ORGANISM: Ruminiclostridium josui (Clostridium josui) SIMILARITY: In the N-terminal section; belongs to the glutamyl-tRNA reductase family.; SIMILARITY: In the C-terminal section; belongs to the precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase family. FUNCTION: Multifunctional enzyme that catalyzes the NADPH-dependent reduction of glutamyl-tRNA(Glu) to glutamate 1-semialdehyde (GSA), the NAD-dependent ring dehydrogenation of precorrin-2 to sirohydrochlorin and finally, the ferrochelation of sirohydrochlorin to yield siroheme. PATHWAY: Cofactor biosynthesis; adenosylcobalamin biosynthesis; sirohydrochlorin from precorrin-2: step 1/1.; PATHWAY: Porphyrin-containing compound metabolism; siroheme biosynthesis; siroheme from sirohydrochlorin: step 1/1.; PATHWAY: Porphyrin-containing compound metabolism; siroheme biosynthesis; sirohydrochlorin from precorrin-2: step 1/1.; PATHWAY: Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from L-glutamyl-tRNA(Glu): step 1/2. KEYWORDS: Cobalamin biosynthesis;Lyase;Multifunctional enzyme;NAD;NADP;Oxidoreductase;Porphyrin biosynthesis MISCELLANEOUS: During catalysis, the active site Cys acts as a nucleophile attacking the alpha-carbonyl group of tRNA-bound glutamate with the formation of a thioester intermediate between enzyme and glutamate, and the concomitant release of tRNA(Glu). The thioester intermediate is finally reduced by direct hydride transfer from NADPH, to form the product GSA." Q59465,"PROTEIN NAMES: Cadmium, zinc and cobalt-transporting ATPase PROTEIN FAMILY: Cation transport ATPase (P-type) family, Type IB subfamily ORGANISM: Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) SIMILARITY: Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily. FUNCTION: Couples the hydrolysis of ATP with the transport of cadmium, zinc and cobalt out of the cell. This ion efflux may influence the activity of urease, which is essential for the survival of the bacterium in the gastric environment. KEYWORDS: ATP-binding;Cadmium;Cell membrane;Cobalt;Magnesium;Membrane;Metal-binding;Nucleotide-binding;Phosphoprotein;Reference proteome;Translocase;Transmembrane;Transmembrane helix;Zinc SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. " Q59771,PROTEIN NAMES: Phenylalanine dehydrogenase (PheDH) PROTEIN FAMILY: Glu/Leu/Phe/Val dehydrogenases family ORGANISM: Rhodococcus sp SIMILARITY: Belongs to the Glu/Leu/Phe/Val dehydrogenases family. FUNCTION: Catalyzes the reversible NAD(+)-dependent oxidative deamination of L-phenylalanine to phenylpyruvate. PATHWAY: Amino-acid biosynthesis; L-phenylalanine biosynthesis; L-phenylalanine from phenylpyruvate (PDH route): step 1/1. KEYWORDS: 3D-structure;Direct protein sequencing;NAD;Nucleotide-binding;Oxidoreductase Q59DX8,"PROTEIN NAMES: N-alpha-acetyltransferase 80 (DmNAA80) PROTEIN FAMILY: Acetyltransferase family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the acetyltransferase family. FUNCTION: N-alpha-acetyltransferase that acetylates the amino terminal acidic residue of proteins devoid of initiator methionine. Preferentially acts on proteins starting with Asp-Asp-Asp and Glu-Glu-Glu sequences. In vitro, shows high activity towards N-terminal sequences starting with Met-Asp-Glu-Leu, Met-Glu-Glu-Glu and Met-Asp-Asp-Asp. KEYWORDS: 3D-structure;Acyltransferase;Alternative splicing;Reference proteome;Transferase " Q59E36,"PROTEIN NAMES: REST corepressor (CoREST) PROTEIN FAMILY: CoREST family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the CoREST family. FUNCTION: Essential component of a corepressor complex that represses transcription of neuron-specific genes in non-neuronal cells. The BHC complex is recruited by Ttk88 and probably acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. May serve as a molecular beacon for the recruitment of molecular machinery that imposes silencing across a chromosomal interval. KEYWORDS: Alternative splicing;Chromatin regulator;Coiled coil;Nucleus;Phosphoprotein;Reference proteome;Repeat;Repressor;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " Q59KM8,"PROTEIN NAMES: Cell cycle protein kinase DBF2 (Dumbbell forming protein 2) PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family ORGANISM: Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. FUNCTION: Ser/Thr-protein kinase involved in the mitotic exit network (MEN) and required after the metaphase to anaphase cell cycle transition. Required for proper nuclear segregation, mitotic spindle organization, actomyosin ring contraction, primary septum assembly, and normal hyphal morphogenesis. KEYWORDS: ATP-binding;Cell cycle;Cell division;Cytoplasm;Cytoskeleton;Kinase;Nucleotide-binding;Nucleus;Phosphoprotein;Reference proteome;Serine/threonine-protein kinase;Transferase SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body Cytoplasm, cytoskeleton, spindle Nucleus Bud neck Note=In small unbudded G1 cells, localizes to the spindle pole body (SPB). At the G1/S transition, remains associated with the SPB but also starts to accumulate in a linear structure in the nuclei of the cells. During mitosis, a very faint signal along the mother-bud axis is observed, suggesting association with the mitotic spindle during this part of the cell cycle. Finally, localizes to the bud neck at the end of mitosis and cytokinesis. " Q59NX5,"PROTEIN NAMES: Filamentous growth regulator 27 ORGANISM: Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) FUNCTION: Transcription factor involved in yeast cell adherence to silicone substrate, filamentous growth, and biofilm formation. KEYWORDS: Cell adhesion;Coiled coil;DNA-binding;Metal-binding;Nucleus;Reference proteome;Transcription;Transcription regulation;Zinc SUBCELLULAR LOCATION: Nucleus " Q59SF7,"PROTEIN NAMES: Cell wall mannoprotein PIR1 (Protein with internal repeats 1) PROTEIN FAMILY: PIR protein family ORGANISM: Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) SIMILARITY: Belongs to the PIR protein family. FUNCTION: Component of the outer cell wall layer required for stability of the cell wall and specifically for cell wall rigidity. KEYWORDS: Cell wall;Cell wall biogenesis/degradation;Glycoprotein;Reference proteome;Repeat;Secreted;Signal SUBCELLULAR LOCATION: Secreted, cell wall Note=Covalently attached to the cell wall. " Q59TP1,"PROTEIN NAMES: Cell wall protein RBT1 (Repressed by TUP1 protein 1) PROTEIN FAMILY: HWP1 family ORGANISM: Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) SIMILARITY: Belongs to the HWP1 family. FUNCTION: GPI-anchored cell wall protein required for mating efficiency, biofilm formation, and virulence. Involved in normal disseminated infection, but not in intestinal colonization. KEYWORDS: Cell wall;Glycoprotein;GPI-anchor;Lipoprotein;Membrane;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted, cell wall Membrane; Lipid-anchor, GPI-anchor. " Q59UP6,"PROTEIN NAMES: GPI-anchored hemophore PGA10 (GPI-anchored protein 10) (Repressed by TUP1 protein 51) (Repressed by TUP1 protein 8) PROTEIN FAMILY: RBT5 family ORGANISM: Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) SIMILARITY: Belongs to the RBT5 family. FUNCTION: Heme-binding protein involved in heme-iron utilization. The ability to acquire iron from host tissues is a major virulence factor of pathogenic microorganisms. Involved in biofilm formation. KEYWORDS: Cell membrane;Cell wall;Disulfide bond;Glycoprotein;GPI-anchor;Heme;Iron;Lipoprotein;Membrane;Metal-binding;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted, cell wall Cell membrane ; Lipid-anchor, GPI-anchor Note=Found anchored in the cell membrane as well as a covalently-linked GPI-modified cell wall protein (GPI-CWP). " Q59UT5,"PROTEIN NAMES: GPI-anchored hemophore PGA7 (Predicted GPI-anchored protein 7) (Repressed by TUP1 protein 6) PROTEIN FAMILY: RBT5 family ORGANISM: Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) SIMILARITY: Belongs to the RBT5 family. FUNCTION: GPI-linked hyphal surface heme-binding protein involved in heme-iron utilization. Heme transfer occurs between PGA7, RBT5 and CSA2 supporting a model in which the 3 CFEM proteins cooperate in a heme-acquisition system and form a cross-cell wall heme-transfer cascade. The ability to acquire iron from host tissues is a major virulence factor of pathogenic microorganisms. Required for biofilm formation. KEYWORDS: Cell membrane;Cell wall;Disulfide bond;Glycoprotein;GPI-anchor;Heme;Iron;Lipoprotein;Membrane;Metal-binding;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted, cell wall Cell membrane ; Lipid-anchor, GPI-anchor " Q59VF4,PROTEIN NAMES: Histone acetyltransferase type B catalytic subunit PROTEIN FAMILY: HAT1 family ORGANISM: Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) SIMILARITY: Belongs to the HAT1 family. FUNCTION: Catalytic component of the histone acetylase B (HAT-B) complex. Acetylates 'Lys-14' of histone H4 which is required for telomeric silencing. Has intrinsic substrate specificity that modifies lysine in recognition sequence GXGKXG. Involved in DNA double-strand break repair. KEYWORDS: Acyltransferase;Chromatin regulator;Cytoplasm;DNA damage;DNA repair;Nucleus;Reference proteome;Transferase SUBCELLULAR LOCATION: Cytoplasm Nucleus Q59VQ3,"PROTEIN NAMES: Spindle assembly checkpoint component MAD2 PROTEIN FAMILY: MAD2 family ORGANISM: Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) SIMILARITY: Belongs to the MAD2 family. FUNCTION: Central component of the spindle assembly checkpoint which is a feedback control that prevents cells with incompletely assembled spindles from leaving mitosis. Plays a key role in virulence, probably through cell cycle checkpoint functions, especially those monitoring the integrity of DNA and chromosome segregation, which might be required for the pathogen to repair damage caused by host defense. KEYWORDS: Cell cycle;Cell division;Mitosis;Nucleus;Reference proteome;Virulence SUBCELLULAR LOCATION: Nucleus " Q59VX8,"PROTEIN NAMES: Septation protein 7 (Seventh homolog of septin 1) PROTEIN FAMILY: TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily, Septin GTPase family ORGANISM: Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) SIMILARITY: Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family. FUNCTION: Septins are GTPases involved in cytokinesis that assemble early in the cell cycle as a patch at the incipient bud site and form a ring before bud emergence, which transforms into an hour-glass shaped collar of cortical filaments that spans both sides of the mother-bud neck. This collar persists until just before cytokinesis, when it splits into two rings that occupy opposite sides of the neck. The septins at the bud neck serve as a structural scaffold that recruits different components involved in diverse processes at specific stages during the cell cycle. Many proteins bind asymmetrically to the septin collar. The septin assembly is regulated by protein kinase GIN4. Septins are also involved in cell morphogenesis, chlamydospores morphogenesis, bud site selection, chitin deposition, cell cycle regulation, cell compartmentalization and spore wall formation. SEP7 is required to convert hyphal septin rings into the hyphal-specific state and is necessary for CDC10 turnover during hyphal growth. KEYWORDS: Cell cycle;Cell division;Coiled coil;GTP-binding;Hydrolase;Nucleotide-binding;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Bud neck Note=Present at the bud neck during cell division. " Q59WH0,"PROTEIN NAMES: Transcriptional adapter 2 ORGANISM: Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) FUNCTION: Functions as a component of the transcription regulatory histone acetylation (HAT) complexes SAGA, SALSA and ADA. SAGA is involved in RNA polymerase II-dependent transcriptional regulation of approximately 10% of yeast genes. At the promoters, SAGA is required for recruitment of the basal transcription machinery. SAGA acetylates nucleosomal histone H3 to some extent (to form H3K9ac, H3K14ac, H3K18ac and H3K23ac). SAGA interacts with DNA via upstream activating sequences (UASs). SALSA, an altered form of SAGA, may be involved in positive transcriptional regulation. ADA preferentially acetylates nucleosomal histones H3 (to form H3K14ac and H3K18ac) and H2B. Required for expression of many CAS5-dependent genes. Plays a key role in cell wall integrity, cell adhesion, hyphal development and pathogenesis. KEYWORDS: Cell adhesion;Cell wall biogenesis/degradation;Metal-binding;Nucleus;Reference proteome;Transcription;Transcription regulation;Virulence;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus " Q59Y20,PROTEIN NAMES: Protein DSE1 (Daughter-specific expression protein 1) PROTEIN FAMILY: WD repeat DSE1 family ORGANISM: Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) SIMILARITY: Belongs to the WD repeat DSE1 family. FUNCTION: Involved in cell wall metabolism and required for the separation of the mother and daughter cells. KEYWORDS: Cell cycle;Cell division;Cell wall biogenesis/degradation;Reference proteome;Repeat;WD repeat Q59Y31,"PROTEIN NAMES: Yeast-form wall Protein 1 (GPI-anchored protein 24) (Thiol-extractable peptide 1) (flocculation protein 1) PROTEIN FAMILY: Flocculin family ORGANISM: Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) SIMILARITY: Belongs to the flocculin family. FUNCTION: Cell wall protein which plays an anti-adhesive role and promotes dispersal of yeast forms, which allows the organism to seek new sites for colonization. KEYWORDS: Cell adhesion;Cell wall;Direct protein sequencing;Glycoprotein;GPI-anchor;Lipoprotein;Membrane;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted. Secreted, cell wall. Membrane ; Lipid-anchor, GPI-anchor Note=In yeast cultures, is linked covalently to glucans of the wall matrix, but, as cultures approach stationary phase, accumulates in the medium and is extractable from cells with disulfide-reducing agents. Removed from the cell wall by proteolytic cleavage by SAP9 and SAP10. " Q5A0E5,"PROTEIN NAMES: Transcriptional regulator NRG1 ORGANISM: Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) FUNCTION: Transcriptional repressor that binds NRG1 response elements (NRE) of target promoters. Involved in regulation of chlamydospore formation, hyphal growth, virulence, and stress response. Plays a key role in regulating true hyphal growth, but does not regulate pseudohyphal growth in the same fashion. Directs transcriptional repression of a subset of filament-specific genes such as HWP1, HYR1, ALS8, HWP1, or ECE1; via the TUP1 pathway. Functions with UME6 in a negative feedback loop to control the level and duration of filament-specific gene expression in response to inducing conditions. Plays a key role in biofilm formation and dispersion. Also plays the role of a negative regulator of virulence in mice models. Required for the expression of the cell wall genes RBR1. KEYWORDS: Metal-binding;Nucleus;Reference proteome;Repeat;Repressor;Stress response;Virulence;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus " Q5A1W9,"PROTEIN NAMES: NAD-dependent histone deacetylase HST3 (Homologous to SIR2 protein 3) (Regulatory protein SIR2 homolog 3) PROTEIN FAMILY: Sirtuin family, Class I subfamily ORGANISM: Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) SIMILARITY: Belongs to the sirtuin family. Class I subfamily. FUNCTION: NAD-dependent histone deacetylase, which could function in telomeric silencing, cell cycle progression and chromosome stability. KEYWORDS: Cytoplasm;Metal-binding;NAD;Nucleus;Reference proteome;Repressor;Transcription;Transcription regulation;Transferase;Zinc SUBCELLULAR LOCATION: Cytoplasm Nucleus " Q5A343,"PROTEIN NAMES: GPI-anchored protein 13 ORGANISM: Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) FUNCTION: Cell wall protein which contributes to cell wall synthesis and is important for acquiring normal surface properties. Required for virulence in a mouse infection model. KEYWORDS: Cell wall;Cell wall biogenesis/degradation;Glycoprotein;GPI-anchor;Lipoprotein;Membrane;Reference proteome;Secreted;Signal;Virulence SUBCELLULAR LOCATION: Secreted, cell wall Membrane ; Lipid-anchor, GPI-anchor Note=Covalently-linked GPI-modified cell wall protein (GPI-CWP). " Q5A446,"PROTEIN NAMES: Ferric/cupric reductase transmembrane component 1 (Ferric-chelate reductase 1) PROTEIN FAMILY: Ferric reductase (FRE) family ORGANISM: Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) SIMILARITY: Belongs to the ferric reductase (FRE) family. FUNCTION: Ferric reductase responsible for reducing extracellular iron and copper prior to import. Catalyzes the reductive uptake of Fe(3+)-salts and Fe(3+) bound to catecholate or hydroxamate siderophores. Fe(3+) is reduced to Fe(2+), which then dissociates from the siderophore and can be imported by the high-affinity Fe(2+) transport complex in the plasma membrane. Also participates in Cu(2+) reduction and Cu(+) uptake (By similarity). Involved in maintenance of cell wall integrity (CWI), mitochondrial function, and interaction between the pathogen and the host. KEYWORDS: Cell membrane;Copper;Copper transport;Electron transport;FAD;Flavoprotein;Glycoprotein;Heme;Ion transport;Iron;Iron transport;Membrane;Metal-binding;NADP;Oxidoreductase;Reference proteome;Signal;Transmembrane;Transmembrane helix;Transport;Virulence SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein " Q5A4E3,"PROTEIN NAMES: Initiation-specific alpha-1,6-mannosyltransferase (Outer chain elongation protein 1) PROTEIN FAMILY: Glycosyltransferase 32 family ORGANISM: Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) SIMILARITY: Belongs to the glycosyltransferase 32 family. FUNCTION: Mannosyltransferase involved in outer chain elongation of asparagine-linked oligosaccharides of the type Man(9)GlcNAc(2). Adds the first alpha-1,6-mannose to the Man(8)GlcNAc(2) and Man(9)GlcNAc(2), but not Man(5)GlcNAc(2), endoplasmic reticulum intermediates (By similarity). Represents the first enzymatic event required for synthesis of outer chain mannose linkages on yeast secretory proteins. N-glycan outer chain epitopes play a crucial role in the host-fungal interaction, virulence, and host immune response such as interleukin synthesis or phagocytosis by neutrophils. KEYWORDS: Endoplasmic reticulum;Glycosyltransferase;Golgi apparatus;Membrane;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix;Virulence SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Single-pass type II membrane protein Golgi apparatus membrane ; Single-pass type II membrane protein Note=Is recycled between the trans-Golgi network and a late compartment of the endoplasmic reticulum. " Q5A4M8,"PROTEIN NAMES: Protein SUR7 PROTEIN FAMILY: SUR7 family ORGANISM: Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) SIMILARITY: Belongs to the SUR7 family. FUNCTION: Involved in cell wall, plasma membrane, and cytoskeletal organization. Plays a role in endocytosis and hyphal morphogenesis. Required to restrict septin proteins to the bud neck and prevents intracellular growth of cell wall. Contributes to secretion, biofilm formation, and macrophage killing. Essential for resistance to stressful conditions and for invasive growth and virulence. KEYWORDS: Cell membrane;Cell wall biogenesis/degradation;Endocytosis;Glycoprotein;Membrane;Phosphoprotein;Reference proteome;Signal;Stress response;Transmembrane;Transmembrane helix;Virulence SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Note=Localizes at eisosomes, structures which colocalize with sites of protein and lipid endocytosis. " Q5A7S7,PROTEIN NAMES: Fork-head transcriptional regulator 2 ORGANISM: Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) FUNCTION: Transcription factor required for the morphogenesis of true hyphal as well as yeast cells. Contributes to virulence. KEYWORDS: DNA-binding;Nucleus;Reference proteome;Transcription;Transcription regulation;Virulence SUBCELLULAR LOCATION: Nucleus Q5AAJ8,"PROTEIN NAMES: Histone acetyltransferase RTT109 PROTEIN FAMILY: RTT109 family ORGANISM: Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) SIMILARITY: Belongs to the RTT109 family. FUNCTION: Histone chaperone-dependent acetylase that modifies 'Lys-56' of histone H3 (H3K56ac), to promote genomic stability, DNA repair and transcriptional regulation during mitotic S-phase. Plays an important role in the regulation of white-opaque genotoxin induced-switching. KEYWORDS: 3D-structure;Acetylation;DNA damage;Nucleus;Reference proteome;Transcription;Transcription regulation;Transferase;Virulence SUBCELLULAR LOCATION: Nucleus " Q5AD05,"PROTEIN NAMES: Cell surface superoxide dismutase [Cu-Zn] 4 (GPI-anchored protein 2) PROTEIN FAMILY: Cu-Zn superoxide dismutase family ORGANISM: Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) SIMILARITY: Belongs to the Cu-Zn superoxide dismutase family. FUNCTION: Superoxide dismutases serve to convert damaging superoxide radicals, a key form of ROS, to less damaging hydrogen peroxide that can be converted into water by catalase action. Degrades host-derived reactive oxygen species to escape innate immune surveillance. Involved in the occurrence of miconazole-tolerant persisters in biofilms. Persisters are cells that survive high doses of an antimicrobial agent. KEYWORDS: Antioxidant;Cell wall;Copper;Glycoprotein;GPI-anchor;Lipoprotein;Membrane;Metal-binding;Oxidoreductase;Reference proteome;Secreted;Signal;Virulence;Zinc SUBCELLULAR LOCATION: Secreted, cell wall Membrane ; Lipid-anchor, GPI-anchor. Note=Covalently-linked GPI-modified cell wall protein (GPI-CWP). " Q5AKU6,"PROTEIN NAMES: Oxidative stress response two-component system protein SSK1 ORGANISM: Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) FUNCTION: Final receptor of the SLN1-YPD1-SSK1 two-component regulatory system, which controls activity of the HOG1 pathway in response to oxidative stress and probably also to the osmolarity of the extracellular environment. Involved in cell wall biosynthesis, hyphal growth, and virulence. Regulates the expression of CHK1, as well as of a subset of genes whose functions are associated with cell wall biosynthesis and adaptation to oxidative stress. Provides at least partial adaptive functions for the survival following encounter with human neutrophils. KEYWORDS: Phosphoprotein;Reference proteome;Stress response;Two-component regulatory system;Virulence " Q5AKZ2,"PROTEIN NAMES: Membrane-bound O-acyltransferase GUP1 (Glycerol uptake protein 1) PROTEIN FAMILY: Membrane-bound acyltransferase family ORGANISM: Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) SIMILARITY: Belongs to the membrane-bound acyltransferase family. FUNCTION: Membrane-bound O-acyltransferase involved in the remodeling of glycosylphosphatidylinositol (GPI) anchors. Acts only on GPI-anchored proteins, but not on free GPI lipids. Also involved in lipid metabolism, having profound effects on sphingolipid-sterol-ordered domains integrity and assembly. Involved in cell integrity and apoptosis (By similarity). Plays a role in virulence and antifungal resistance. KEYWORDS: Acyltransferase;Cell membrane;Endoplasmic reticulum;Membrane;Mitochondrion;Reference proteome;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Endoplasmic reticulum membrane ; Multi-pass membrane protein Mitochondrion membrane ; Multi-pass membrane protein " Q5ANC9,"PROTEIN NAMES: Autophagy-related protein 9 PROTEIN FAMILY: ATG9 family ORGANISM: Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) SIMILARITY: Belongs to the ATG9 family. FUNCTION: Phospholipid scramblase involved in autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation. Cycles between the preautophagosomal structure/phagophore assembly site (PAS) and the cytoplasmic vesicle pool and supplies membrane for the growing autophagosome. Lipid scramblase activity plays a key role in preautophagosomal structure/phagophore assembly by distributing the phospholipids that arrive through atg2 from the cytoplasmic to the luminal leaflet of the bilayer, thereby driving autophagosomal membrane expansion. Required for mitophagy. Also involved in endoplasmic reticulum-specific autophagic process and is essential for the survival of cells subjected to severe ER stress. Different machineries are required for anterograde trafficking to the PAS during either the Cvt pathway or bulk autophagy and for retrograde trafficking (By similarity). KEYWORDS: Autophagy;Cytoplasmic vesicle;Endoplasmic reticulum;Golgi apparatus;Lipid transport;Membrane;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Preautophagosomal structure membrane ; Multi-pass membrane protein Cytoplasmic vesicle membrane ; Multi-pass membrane protein Golgi apparatus membrane ; Multi-pass membrane protein Endoplasmic reticulum membrane ; Multi-pass membrane protein " Q5ANJ4,"PROTEIN NAMES: Transcription activator TEC1 PROTEIN FAMILY: TEC1 family ORGANISM: Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) SIMILARITY: Belongs to the TEC1 family. FUNCTION: Transcription factor which regulates genes involved in hyphal development, cell adhesion, biofilm development, and virulence. Plays a role in the formation of 'finger' morphology, a unique multicellular morphology of C.albicans induced by carbon dioxide. Regulates gene expression during intestinal colonization. Required for the expression of the secreted aspartyl proteinases SAP4, SAP5, and SAP6; but also of BCR1, PGA4, and CDC24. Moreover, a positive feedback loop between CDC24 and TEC1 contributes to an increase in active CDC42 at the tip of the germ tube which is important for hyphae formation. Regulates also the pheromone response of the white cell phenotype. KEYWORDS: Cell adhesion;DNA-binding;Nucleus;Reference proteome;Transcription;Transcription regulation;Virulence SUBCELLULAR LOCATION: Nucleus " Q5AP52,"PROTEIN NAMES: Filamentous growth regulator 41 (GPI-anchored protein 35) ORGANISM: Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) FUNCTION: Putative adhesin which may be involved in cell adhesion and virulence (By similarity). Involved in the regulation of filamentous growth. KEYWORDS: Cell adhesion;Cell membrane;Glycoprotein;GPI-anchor;Lipoprotein;Membrane;Reference proteome;Secreted;Signal;Virulence SUBCELLULAR LOCATION: Secreted Cell membrane ; Lipid-anchor, GPI-anchor " Q5AP95,"PROTEIN NAMES: Suppressor of ferric uptake 1 ORGANISM: Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) FUNCTION: Transcriptional regulator of iron-responsive genes. Represses expression of SEF1 and genes for iron uptake if iron is present. Plays also a transcription-independent role in the direct inhibition of SEF1 function through protein complex formation and translocation to the cytoplasm, where SEF1 is destabilized. Promotes gastrointestinal commensalism in mice. KEYWORDS: Cytoplasm;Iron;Metal-binding;Nucleus;Reference proteome;Repeat;Repressor;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cytoplasm Nucleus " Q5AWI5,PROTEIN NAMES: Alpha/beta-glucosidase agdC PROTEIN FAMILY: Glycosyl hydrolase 31 family ORGANISM: Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) SIMILARITY: Belongs to the glycosyl hydrolase 31 family. FUNCTION: Glucosidase involved in the degradation of cellulosic biomass. Has both alpha- and beta-glucosidase activity. KEYWORDS: Carbohydrate metabolism;Cell wall biogenesis/degradation;Glycoprotein;Glycosidase;Hydrolase;Polysaccharide degradation;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted Q5B323,"PROTEIN NAMES: Tyrosine-protein phosphatase cdcA PROTEIN FAMILY: Protein-tyrosine phosphatase family, Non-receptor class CDC14 subfamily ORGANISM: Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) SIMILARITY: Belongs to the protein-tyrosine phosphatase family. Non-receptor class CDC14 subfamily. FUNCTION: Protein phosphatase which antagonizes mitotic cyclin-dependent kinase nimX, the inactivation of which is essential for exit from mitosis. To access its substrates, is released from nucleolar sequestration during mitosis. Plays an essential in coordinating the nuclear division cycle with cytokinesis through the cytokinesis checkpoint. Involved in chromosome segregation, where it is required for meiosis I spindle dissambly as well as for establishing two consecutive chromosome segregation phases (By similarity). Required for the transcription of the two major endoglucanase genes eglA and eglB and growth on synthetic cellulose as the sole carbon source. KEYWORDS: Cell cycle;Cell division;Cytoplasm;Hydrolase;Meiosis;Mitosis;Nucleus;Phosphoprotein;Protein phosphatase;Reference proteome SUBCELLULAR LOCATION: Nucleus Cytoplasm Cell septum Note=Does not localize to the nucleolus as its homologs do in S.cerevisiae and S.pombe. Accumulates within nuclei as they pass through interphase, displays a complex localization in mitosis, and localizes to the forming septum after mitosis is completed. " Q5BJH7,"PROTEIN NAMES: Protein YIF1B (YIP1-interacting factor homolog B) PROTEIN FAMILY: YIF1 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the YIF1 family. FUNCTION: Functions in endoplasmic reticulum to Golgi vesicle-mediated transport and regulates the proper organization of the endoplasmic reticulum and the Golgi (By similarity). Plays a key role in targeting to neuronal dendrites receptors such as HTR1A (By similarity). Plays also a role in primary cilium and sperm flagellum assembly probably through protein transport to these compartments. KEYWORDS: Acetylation;Alternative splicing;Disease variant;Endoplasmic reticulum;Golgi apparatus;Intellectual disability;Membrane;Phosphoprotein;Protein transport;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein Golgi apparatus membrane ; Multi-pass membrane protein Endoplasmic reticulum-Golgi intermediate compartment membrane ; Multi-pass membrane protein Note=Shuttles between the endoplasmic reticulum, the intermediate compartment and the Golgi apparatus. " Q5BKZ1,"PROTEIN NAMES: DBIRD complex subunit ZNF326 (Zinc finger protein 326) (Zinc finger protein interacting with mRNPs and DBC1) PROTEIN FAMILY: AKAP95 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the AKAP95 family. FUNCTION: Core component of the DBIRD complex, a multiprotein complex that acts at the interface between core mRNP particles and RNA polymerase II (RNAPII) and integrates transcript elongation with the regulation of alternative splicing: the DBIRD complex affects local transcript elongation rates and alternative splicing of a large set of exons embedded in (A + T)-rich DNA regions. May play a role in neuronal differentiation and is able to bind DNA and activate expression in vitro. KEYWORDS: Acetylation;Activator;Alternative splicing;DNA-binding;Isopeptide bond;Metal-binding;Methylation;mRNA processing;mRNA splicing;Nucleus;Phosphoprotein;Reference proteome;Repeat;Transcription;Transcription regulation;Ubl conjugation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus matrix " Q5BL29,"PROTEIN NAMES: Zinc transporter ZIP9 (Solute carrier family 39 member 9) (Zrt- and Irt-like protein 9) (ZIP-9) PROTEIN FAMILY: ZIP transporter family ORGANISM: Danio rerio (Zebrafish) (Brachydanio rerio) SIMILARITY: Belongs to the ZIP transporter (TC 2.A.5) family. FUNCTION: Has dual functions as a membrane-bound androgen receptor and as an androgen-dependent zinc transporter both of which are mediated through G protein activation and are required for the androgen-dependent apoptotic response. Upon androgen binding, mediates apoptosis by directly activating a stimulatory G protein that leads to increased cAMP levels and MAP kinase activity and which is accompanied by increased intracellular free zinc levels (By similarity). May play a role in the proper zinc modulation during egg activation. KEYWORDS: Cell membrane;Cytoplasm;Glycoprotein;Golgi apparatus;Ion transport;Membrane;Mitochondrion;Nucleus;Reference proteome;Transmembrane;Transmembrane helix;Transport;Zinc;Zinc transport SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Cytoplasm, perinuclear region Golgi apparatus, trans-Golgi network membrane Mitochondrion Nucleus " Q5CZ52,"PROTEIN NAMES: Bardet-Biedl syndrome 4 protein homolog PROTEIN FAMILY: BBS4 family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the BBS4 family. FUNCTION: Component of the BBSome complex (By similarity). The BBSome complex is thought to function as a coat complex required for sorting of specific membrane proteins to the primary cilia (By similarity). The BBSome complex is required for ciliogenesis but is dispensable for centriolar satellite function (By similarity). Required for proper BBSome complex assembly and its ciliary localization (By similarity). May be required for microtubule anchoring at the centrosome but not for microtubule nucleation (By similarity). May be required for the dynein-mediated transport of pericentriolar proteins to the centrosome (By similarity). Required, redundantly with bbs-5, for cilia biogenesis and both the assembly and movement of intraflagellar transport proteins along the ciliary axoneme. Plays a role in the removal of degraded mechanosensory receptors within the cilia. KEYWORDS: Alternative splicing;Cell membrane;Cell projection;Cilium;Cilium biogenesis/degradation;Cytoplasm;Cytoskeleton;Membrane;Protein transport;Reference proteome;Repeat;TPR repeat;Transport SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Cytoplasm, cytoskeleton Cell projection, cilium membrane " Q5EA59,"PROTEIN NAMES: (Lyso)-N-acylphosphatidylethanolamine lipase (Alpha/beta hydrolase domain-containing protein 4) (Abhydrolase domain-containing protein 4) (Alpha/beta-hydrolase 4) (Protein ABHD4) PROTEIN FAMILY: Peptidase S33 family, ABHD4/ABHD5 subfamily ORGANISM: Bos taurus (Bovine) SIMILARITY: Belongs to the peptidase S33 family. ABHD4/ABHD5 subfamily. FUNCTION: Lysophospholipase selective for N-acyl phosphatidylethanolamine (NAPE). Contributes to the biosynthesis of N-acyl ethanolamines, including the endocannabinoid anandamide by hydrolyzing the sn-1 and sn-2 acyl chains from N-acyl phosphatidylethanolamine (NAPE) generating glycerophospho-N-acyl ethanolamine (GP-NAE), an intermediate for N-acyl ethanolamine biosynthesis. Hydrolyzes substrates bearing saturated, monounsaturated, polyunsaturated N-acyl chains. Shows no significant activity towards other lysophospholipids, including lysophosphatidylcholine, lysophosphatidylethanolamine and lysophosphatidylserine. KEYWORDS: Hydrolase;Lipid degradation;Lipid metabolism;Reference proteome " Q5EAF2,"PROTEIN NAMES: Type IV inositol polyphosphate 5-phosphatase 11 (At5PTase11) PROTEIN FAMILY: Inositol polyphosphate 5-phosphatase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the inositol polyphosphate 5-phosphatase family. FUNCTION: Has phosphatase activity toward PtdIns(4,5)P2, and in vitro toward PtdIns(3,5)P2 and PtdIns(3,4,5)P3. Cannot dephosphorylate PtdIns(5)P, Ins(1,4,5)P3 and Ins(1,3,4,5)P4. KEYWORDS: Alternative splicing;Cell membrane;Hydrolase;Membrane;Reference proteome SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein " Q5EU90,"PROTEIN NAMES: Trans-2-enoyl-CoA reductase (TER1) PROTEIN FAMILY: TER reductase family ORGANISM: Euglena gracilis SIMILARITY: Belongs to the TER reductase family. FUNCTION: Catalyzes reduction of trans-2-enoyl-CoA to acyl-CoA, an important step in the fatty acid biosynthesis pathway, which is performed in mitochondria under anaerobiosis. Preferably catalyzes the reduction of short chain length substrates such as crotonyl-CoA ((2E)-butenoyl-CoA) and (2E)-hexenoyl-CoA. Can use both NADH and NADPH as electron donor, with 2-3-fold higher specific activities for NADH relative to NADPH. Originally thought to contribute to wax ester production under anaerobic conditions. Later shown to be dispensable for wax ester production under anaerobic conditions, but involved in the greening process (chlorophyll synthesis and/or chloroplast development). PATHWAY: Lipid metabolism; fatty acid metabolism. KEYWORDS: Fatty acid biosynthesis;Fatty acid metabolism;Lipid biosynthesis;Lipid metabolism;Mitochondrion;NAD;NADP;Oxidoreductase;Transit peptide SUBCELLULAR LOCATION: Mitochondrion MISCELLANEOUS: Euglena gracilis exhibits the metabolic behavior of a plant in the light and of an animal in the dark." Q5FBB7,"PROTEIN NAMES: Shugoshin 1 (Serologically defined breast cancer antigen NY-BR-85) (Shugoshin-like 1) PROTEIN FAMILY: Shugoshin family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the shugoshin family. FUNCTION: Plays a central role in chromosome cohesion during mitosis by preventing premature dissociation of cohesin complex from centromeres after prophase, when most of cohesin complex dissociates from chromosomes arms. May act by preventing phosphorylation of the STAG2 subunit of cohesin complex at the centromere, ensuring cohesin persistence at centromere until cohesin cleavage by ESPL1/separase at anaphase. Essential for proper chromosome segregation during mitosis and this function requires interaction with PPP2R1A. Its phosphorylated form is necessary for chromosome congression and for the proper attachment of spindle microtubule to the kinetochore. Necessary for kinetochore localization of PLK1 and CENPF. May play a role in the tension sensing mechanism of the spindle-assembly checkpoint by regulating PLK1 kinetochore affinity. Isoform 3 plays a role in maintaining centriole cohesion involved in controlling spindle pole integrity. Involved in centromeric enrichment of AUKRB in prometaphase. KEYWORDS: 3D-structure;Alternative splicing;Cell cycle;Cell division;Centromere;Chromosome;Chromosome partition;Coiled coil;Cytoplasm;Cytoskeleton;Disease variant;Kinetochore;Mitosis;Nucleus;Phosphoprotein;Reference proteome;Ubl conjugation SUBCELLULAR LOCATION: Nucleus Chromosome, centromere Chromosome, centromere, kinetochore Cytoplasm, cytoskeleton, spindle pole Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Note=Localizes to the inner centromere throughout prophase until metaphase and disappears at anaphase. Centromeric localization requires the presence of BUB1 and the interaction with PPP2R1A. Colocalizes with NEK2 at the kinetochore. Colocalizes with and SS18L1 at the kinetochore. Phosphorylation by AUKRB and the presence of BUB1 are required for localization to the kinetochore. Isoform 1 primarily localizes to kinetochores during G2 phase and mitotic prophase, metaphase, and anaphase and does not appear to be associated with kinetochores during late mitosis. Isoform 3 is found at the centrosome in interphase and at spindle poles in mitosis and its spindle pole localization is PLK1 dependent. Isoform 3 does not localize to kinetochores during any stages of the cell cycle. MISCELLANEOUS: Shugoshin is Japanese for guardian spirit (as it is known to be a protector of centromeric cohesin)." Q5FVN0,"PROTEIN NAMES: Lysophospholipid acyltransferase 5 (LPLAT 5) (1-acylglycerophosphocholine O-acyltransferase) (1-acylglycerophosphoethanolamine O-acyltransferase) (1-acylglycerophosphoserine O-acyltransferase) (Lysophosphatidylcholine acyltransferase 3) (Lyso-PC acyltransferase 3) (Membrane-bound O-acyltransferase domain-containing protein 5) (O-acyltransferase domain-containing protein 5) PROTEIN FAMILY: Membrane-bound acyltransferase family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the membrane-bound acyltransferase family. FUNCTION: Lysophospholipid O-acyltransferase (LPLAT) that catalyzes the reacylation step of the phospholipid remodeling process also known as the Lands cycle. Catalyzes transfer of the fatty acyl chain from fatty acyl-CoA to 1-acyl lysophospholipid to form various classes of phospholipids. Converts 1-acyl lysophosphatidylcholine (LPC) into phosphatidylcholine (PC) (LPCAT activity), 1-acyl lysophosphatidylserine (LPS) into phosphatidylserine (PS) (LPSAT activity) and 1-acyl lysophosphatidylethanolamine (LPE) into phosphatidylethanolamine (PE) (LPEAT activity). Favors polyunsaturated fatty acyl-CoAs as acyl donors compared to saturated fatty acyl-CoAs (By similarity). Has higher activity for LPC acyl acceptors compared to LPEs and LPSs. Can also transfer the fatty acyl chain from fatty acyl-CoA to 1-O-alkyl lysophospholipid or 1-O-alkenyl lysophospholipid with lower efficiency. Acts as a major LPC O-acyltransferase in liver and intestine. As a component of the liver X receptor/NR1H3 or NR1H2 signaling pathway, mainly catalyzes the incorporation of arachidonate into PCs of endoplasmic reticulum (ER) membranes, increasing membrane dynamics and enabling triacylglycerols transfer to nascent very low-density lipoprotein (VLDL) particles. Promotes processing of sterol regulatory protein SREBF1 in hepatocytes, likely by facilitating the translocation of SREBF1-SCAP complex from ER to the Golgi apparatus. Participates in mechanisms by which the liver X receptor/NR1H3 or NR1H2 signaling pathway counteracts lipid-induced ER stress response and inflammation. Down-regulates hepatic inflammation by limiting arachidonic acid availability for synthesis of inflammatory eicosanoids, such as prostaglandins. In enterocytes, acts as a component of a gut-brain feedback loop that coordinates dietary lipid absorption and food intake. Regulates the abundance of PCs containing linoleate and arachidonate in enterocyte membranes, enabling passive diffusion of fatty acids and cholesterol across the membrane for efficient chylomicron assembly. In the intestinal crypt, acts as a component of dietary-responsive phospholipid-cholesterol axis, regulating the biosynthesis of cholesterol and its mitogenic effects on intestinal stem cells (By similarity). PATHWAY: Lipid metabolism; phospholipid metabolism. KEYWORDS: Acetylation;Acyltransferase;Endoplasmic reticulum;Glycoprotein;Lipid biosynthesis;Lipid metabolism;Membrane;Phospholipid biosynthesis;Phospholipid metabolism;Reference proteome;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein " Q5FVQ0,"PROTEIN NAMES: Metal cation symporter ZIP8 (Solute carrier family 39 member 8) (Zrt- and Irt-like protein 8) (ZIP-8) PROTEIN FAMILY: ZIP transporter family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the ZIP transporter (TC 2.A.5) family. FUNCTION: Electroneutral divalent metal cation:bicarbonate symporter of the plasma membrane mediating the cellular uptake of zinc and manganese, two divalent metal cations important for development, tissue homeostasis and immunity. Transports an electroneutral complex composed of a divalent metal cation and two bicarbonate anions or alternatively a bicarbonate and a selenite anion. Thereby, it also contributes to the cellular uptake of selenium, an essential trace metal and micronutrient. Also imports cadmium a non-essential metal which is cytotoxic and carcinogenic. May also transport iron and cobalt through membranes. Through zinc import, indirectly regulates the metal-dependent transcription factor MTF1 and the expression of some metalloproteases involved in cartilage catabolism and also probably heart development. Also indirectly regulates the expression of proteins involved in cell morphology and cytoskeleton organization. Indirectly controls innate immune function and inflammatory response by regulating zinc cellular uptake which in turn modulates the expression of genes specific of these processes. Protects, for instance, cells from injury and death at the onset of inflammation (By similarity). By regulating zinc influx into monocytes also directly modulates their adhesion to endothelial cells and arteries (By similarity). Reclaims manganese from the bile at the apical membrane of hepatocytes, thereby regulating the activity of the manganese-dependent enzymes through the systemic levels of the nutrient. Also participates in manganese reabsorption in the proximal tubule of the kidney. By mediating the extracellular uptake of manganese by cells of the blood-brain barrier, may also play a role in the transport of the micronutrient to the brain. With manganese cellular uptake also participates in mitochondrial proper function (By similarity). Finally, also probably functions intracellularly, translocating zinc from lysosome to cytosol to indirectly enhance the expression of specific genes during TCR-mediated T cell activation (By similarity). KEYWORDS: Cell membrane;Glycoprotein;Ion transport;Lysosome;Membrane;Reference proteome;Signal;Symport;Transmembrane;Transmembrane helix;Transport;Zinc;Zinc transport SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Lysosome membrane ; Multi-pass membrane protein Apical cell membrane ; Multi-pass membrane protein Basolateral cell membrane ; Multi-pass membrane protein Note=Localizes to the lysosome of activated T-cells. A large fraction of the protein is found intracellularly in microvascular capillary endothelial cells that constitute the blood-brain barrier. Localized and functional at both apical and basolateral membranes of microvascular capillary endothelial cells that constitute the blood-brain barrier. " Q5GC92,PROTEIN NAMES: Maximins-S type B/C [Cleaved into: Maximin-S1; Maximin-S3; Maximin-S5; Maximin-S2; Maximin-S4] PROTEIN FAMILY: Maximin-S family ORGANISM: Bombina maxima (Giant fire-bellied toad) (Chinese red belly toad) SIMILARITY: Belongs to the maximin-S family. FUNCTION: Maximin-S1 has no antimicrobial activity. Has no hemolytic activity.; FUNCTION: Maximin-S2 has an activity against mycoplasma but has no activity against common Gram-positive and Gram-negative bacteria nor fungi. Has no hemolytic activity (By similarity).; FUNCTION: Maximin-S3 has an activity against mycoplasma but has no activity against common Gram-positive and Gram-negative bacteria nor fungi. Has no hemolytic activity (By similarity).; FUNCTION: Maximin-S4 has an activity against mycoplasma but has no activity against common Gram-positive and Gram-negative bacteria nor fungi. Has no hemolytic activity.; FUNCTION: Maximin-S5 has an activity against mycoplasma but has no activity against common Gram-positive and Gram-negative bacteria nor fungi. Has no hemolytic activity (By similarity). KEYWORDS: Alternative splicing;Amidation;Antibiotic;Antimicrobial;Cleavage on pair of basic residues;Secreted;Signal SUBCELLULAR LOCATION: Secreted. Q5HZ36,"PROTEIN NAMES: GATA transcription factor 21 (Protein GATA, NITRATE-INDUCIBLE, CARBON-METABOLISM INVOLVED) (AtGNC) PROTEIN FAMILY: Type IV zinc-finger family, Class B subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the type IV zinc-finger family. Class B subfamily. FUNCTION: Transcriptional regulator that specifically binds 5'-GATA-3' or 5'-GAT-3' motifs within gene promoters. Involved in the modulation of chloroplast development, growth and division in a cytokinin-dependent manner. Repressor of the gibberellic acid (GA) signaling pathway that represses flowering and modulates greening, in a SOC1-dependent manner. Prevents the accumulation of SOC1 during flowering. Promotes chlorophyll biosynthesis throughout the plant, by regulating chlorophyll biosynthetic genes (e.g. HEMA1 and GUN4) and chloroplast localized glutamate synthase (e.g. GLU1). Involved in the regulation of sugar-sensing genes (e.g. HXK1, HXK2, STP13 and PLT6). Regulator of germination, senescence, elongation growth and flowering time. Influences also leaf starch content. KEYWORDS: Alternative splicing;Cytokinin signaling pathway;DNA-binding;Gibberellin signaling pathway;Metal-binding;Nucleus;Reference proteome;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: [Isoform 2]: May be due to a competing acceptor splice site." Q5HZ38,"PROTEIN NAMES: Serine/threonine-protein kinase GRIK2 (Protein GEMINIVIRUS REP INTERACTING KINASE 2) (Protein GRIK2) (SnRK1-activating protein kinase 1) (AtSnAK1) PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. FUNCTION: Activates SnRK1.1/KIN10 and SnRK1.2/KIN11 by phosphorylation of their activation-loop 'Thr-198' and 'Thr-176', respectively. Required for the regulation by SnRK1 kinases of the transcription of a large set of genes, the modification the activity of metabolic enzymes, and the control of various nutrient-responsive cellular developmental processes. KEYWORDS: ATP-binding;Host-virus interaction;Kinase;Nucleotide-binding;Phosphoprotein;Reference proteome;Serine/threonine-protein kinase;Transferase MISCELLANEOUS: Functionally able to complement the yeast elm1 sak1 tos3 triple mutant." Q5HZT0,"PROTEIN NAMES: Decapping and exoribonuclease protein (DXO) (5'-3' exoribonuclease DXO) (Dom-3 homolog Z) (NAD-capped RNA hydrolase DXO) (DeNADding enzyme DXO) PROTEIN FAMILY: DXO/Dom3Z family ORGANISM: Xenopus laevis (African clawed frog) SIMILARITY: Belongs to the DXO/Dom3Z family. FUNCTION: Decapping enzyme for NAD-capped RNAs: specifically hydrolyzes the nicotinamide adenine dinucleotide (NAD) cap from a subset of RNAs by removing the entire NAD moiety from the 5'-end of an NAD-capped RNA. The NAD-cap is present at the 5'-end of some RNAs and snoRNAs. In contrast to the canonical 5'-end N7 methylguanosine (m7G) cap, the NAD cap promotes mRNA decay. Also acts as a non-canonical decapping enzyme that removes the entire cap structure of m7G capped or incompletely capped RNAs and mediates their subsequent degradation. Specifically degrades pre-mRNAs with a defective 5'-end m7G cap and is part of a pre-mRNA capping quality control. Has decapping activity toward incomplete 5'-end m7G cap mRNAs such as unmethylated 5'-end-capped RNA (cap0), while it has no activity toward 2'-O-ribose methylated m7G cap (cap1). Also has 5'-3' exoribonuclease activities: The 5'-end monophosphate RNA is then degraded by the 5'-3' exoribonuclease activity, enabling this enzyme to decap and degrade incompletely capped mRNAs. Also possesses RNA 5'-pyrophosphohydrolase activity by hydrolyzing the 5'-end triphosphate to release pyrophosphates. Exhibits decapping activity towards FAD-capped RNAs (By similarity). Exhibits decapping activity towards dpCoA-capped RNAs in vitro (By similarity). KEYWORDS: Exonuclease;Hydrolase;Magnesium;Metal-binding;Nuclease;Nucleotide-binding;Nucleus;Reference proteome;RNA-binding SUBCELLULAR LOCATION: Nucleus " Q5IW40,"PROTEIN NAMES: 2-hydroxyethylphosphonate dioxygenase (Hydroxyethylphosphonate dioxygenase) (Phosphinothricin tripeptide biosynthesis protein D) PROTEIN FAMILY: Non-heme iron-dependent dioxygenase family ORGANISM: Streptomyces viridochromogenes (strain DSM 40736 / JCM 4977 / BCRC 1201 / Tue 494) SIMILARITY: Belongs to the non-heme iron-dependent dioxygenase family. FUNCTION: Non-heme-dependent dioxygenase that catalyzes the conversion of 2-hydroxyethylphosphonate (HEP) to hydroxymethylphosphonate (HMP) in the biosynthesis of phosphinothricin tripeptide (PTT), also known as bialaphos (BA), a natural-product antibiotic and potent herbicide. PTT contains the unusual amino acid phosphinothricin attached to 2 alanine residues. Synthetic phosphinothricin (glufosinate) is a key component of commercial herbicides. PATHWAY: Secondary metabolite biosynthesis; bialaphos biosynthesis. KEYWORDS: 3D-structure;Antibiotic biosynthesis;Dioxygenase;DNA-binding;Iron;Metal-binding;Oxidoreductase;Reference proteome;Repeat MISCELLANEOUS: Mediates the cleavage of the carbon-carbon bond of 2-hydroxyethylphosphonate (HEP) to produce hydroxymethylphosphonate (HMP) and formate without input of electrons or use of any organic cofactors. Reaction was initially supposed to follow a Criegee rearrangement with a phosphorus-based migrating group. However, it was laster shown that it is not the case.; MISCELLANEOUS: Phosphinothricin tripeptide (PTT) herbicide and fosfomycin antibiotic biosynthesis pathways share early steps starting with phosphoenolpyruvate before the pathways diverge after formation of 2-hydroxyethylphosphonate (HEP). HepD is involved in phosphinothricin tripeptide (PTT) herbicide biosynthesis after divergence of the 2 pathways." Q5JDJ7,"PROTEIN NAMES: 1,4-alpha-glucan branching enzyme TK1436 (1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase) (Alpha-(1->4)-glucan branching enzyme) (Branching enzyme) (BE) PROTEIN FAMILY: Glycosyl hydrolase 57 family ORGANISM: Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1)) SIMILARITY: Belongs to the glycosyl hydrolase 57 family. FUNCTION: Catalyzes the formation of branch points in alpha-glucans by cleavage of an alpha-1,4 glycosidic bond and subsequent transfer of the cleaved-off oligosaccharide to a new alpha-1,6 position. The branch chain-length distribution of the reaction products shows degree of polymerization (DP) of 5 to 30, with two local maxima at DP 6 and DP 11. Exhibits an alpha-retaining catalytic mechanism. Does not display alpha-galactosidase or pullulanase activity, since melibiose and pullulan are not substrates. Is not able to catalyze the hydrolysis or transglycosylation of maltoheptaose, suggesting that the TK1436 protein contains neither alpha-amylase nor 4-alpha-glucanotransferase activity. KEYWORDS: 3D-structure;Carbohydrate metabolism;Glycosyltransferase;Reference proteome;Transferase " Q5JFG9,"PROTEIN NAMES: Polyamine aminopropyltransferase (Agmatine aminopropyltransferase) (Cadaverine aminopropyltransferase) (N1-aminopropylagmatine synthase) (Putrescine aminopropyltransferase) (PAPT) (Spermidine synthase) (SPDS) (SPDSY) PROTEIN FAMILY: Spermidine/spermine synthase family ORGANISM: Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1)) SIMILARITY: Belongs to the spermidine/spermine synthase family. FUNCTION: Involved in the biosynthesis of polyamines which are thought to support the growth of thermophilic microorganisms under high-temperature conditions. It seems that long-chain and branched-chain of polyamines effectively stabilize DNA and RNA, respectively. Catalyzes the irreversible transfer of a propylamine group from the amino donor S-adenosylmethioninamine (decarboxy-AdoMet) to agmatine to yield N1-aminopropylagmatine. It can also use cadaverine (1,5-diaminopentane) and putrescine (1,4-diaminobutane) as substrate with a lower activity than that of agmatine. The reaction involves a nucleophilic attack on the C-3 methylene of the propylamine moiety adjacent to the positively charged sulfur of decarboxy-AdoMet. PATHWAY: Amine and polyamine biosynthesis; spermidine biosynthesis; spermidine from putrescine: step 1/1. KEYWORDS: Cytoplasm;Polyamine biosynthesis;Reference proteome;Spermidine biosynthesis;Transferase SUBCELLULAR LOCATION: Cytoplasm MISCELLANEOUS: In T.kodakarensis, two kinds of synthetic pathways from agmatine to spermidine are predicted. One is the pathway via putrescine (pathway I), and the other is that via N1-aminopropylagmatine (pathway II)." Q5JGC2,PROTEIN NAMES: 2-dehydropantoate 2-reductase (Ketopantoate reductase) (KPR) PROTEIN FAMILY: Ketopantoate reductase family ORGANISM: Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1)) SIMILARITY: Belongs to the ketopantoate reductase family. FUNCTION: Catalyzes the NAD(P)H-dependent reduction of ketopantoate into pantoic acid. Prefers NADH rather than NADPH as the electron donor. PATHWAY: Cofactor biosynthesis; coenzyme A biosynthesis. KEYWORDS: 3D-structure;Coenzyme A biosynthesis;Cytoplasm;NAD;NADP;Nucleotide-binding;Oxidoreductase;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Q5JHF1,"PROTEIN NAMES: Pantoate kinase (PoK) PROTEIN FAMILY: GHMP kinase family, PoK subfamily ORGANISM: Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1)) SIMILARITY: Belongs to the GHMP kinase family. PoK subfamily. FUNCTION: Phosphorylates (R)-pantoate to form (R)-4-phosphopantoate in the CoA biosynthesis pathway. Displays broad nucleotide specificity and utilizes ATP, GTP, UTP, and CTP with comparable catalytic efficiencies. PATHWAY: Cofactor biosynthesis; coenzyme A biosynthesis. KEYWORDS: 3D-structure;ATP-binding;Coenzyme A biosynthesis;Kinase;Nucleotide-binding;Reference proteome;Transferase " Q5JHT1,"PROTEIN NAMES: Plant-type L-asparaginase (L-asparagine amidohydrolase) [Cleaved into: L-asparaginase subunit alpha; L-asparaginase subunit beta] PROTEIN FAMILY: Ntn-hydrolase family ORGANISM: Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1)) SIMILARITY: Belongs to the Ntn-hydrolase family. FUNCTION: Catalyzes the hydrolysis of L-asparagine into L-aspartate and ammonia. Also displays D-asparaginase activity, which is about 10% of the L-asparaginase activity. Does not exhibit glutaminase activity. KEYWORDS: Autocatalytic cleavage;Hydrolase;Protease;Reference proteome " Q5JI38,"PROTEIN NAMES: N(1)-aminopropylagmatine ureohydrolase (Agmatinase) (Protein SpeB homolog) PROTEIN FAMILY: Arginase family ORGANISM: Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1)) SIMILARITY: Belongs to the arginase family. FUNCTION: Involved in the biosynthesis of polyamines which are thought to support the growth of thermophilic microorganisms under high-temperature conditions. It seems that long-chain and branched-chain of polyamines effectively stabilize DNA and RNA, respectively. Catalyzes the decarboxylation of N1-(3-aminopropyl)agmatine to yield spermidine and urea. It can also use agmatine to yield putrescine. PATHWAY: Amine and polyamine biosynthesis; spermidine biosynthesis. KEYWORDS: Cytoplasm;Hydrolase;Manganese;Metal-binding;Polyamine biosynthesis;Reference proteome;Spermidine biosynthesis SUBCELLULAR LOCATION: Cytoplasm MISCELLANEOUS: In T.kodakarensis, two kinds of synthetic pathways from agmatine to spermidine are predicted. One is the pathway via putrescine (pathway I), and the other is that via N1-aminopropylagmatine (pathway II)." Q5JPI9,"PROTEIN NAMES: EEF1A lysine methyltransferase 2 (Methyltransferase-like protein 10) (Protein-lysine N-methyltransferase METTL10) PROTEIN FAMILY: Class I-like SAM-binding methyltransferase superfamily, EFM4 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase superfamily. EFM4 family. FUNCTION: Protein-lysine methyltransferase that selectively catalyzes the trimethylation of EEF1A at 'Lys-318'. KEYWORDS: Acetylation;Cytoplasm;Methyltransferase;Nucleus;Phosphoprotein;Reference proteome;S-adenosyl-L-methionine;Transferase SUBCELLULAR LOCATION: Cytoplasm Nucleus " Q5JQC9,"PROTEIN NAMES: A-kinase anchor protein 4 (AKAP-4) (A-kinase anchor protein 82 kDa) (AKAP 82) (hAKAP82) (Major sperm fibrous sheath protein) (HI) (Protein kinase A-anchoring protein 4) (PRKA4) PROTEIN FAMILY: AKAP110 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the AKAP110 family. FUNCTION: Major structural component of sperm fibrous sheath. Plays a role in sperm motility. KEYWORDS: Alternative splicing;Cell projection;Cilium;Cilium biogenesis/degradation;Flagellum;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cell projection, cilium, flagellum Note=Localizes to the principle piece of the sperm flagellum. " Q5JR98,"PROTEIN NAMES: Dynein light chain Tctex-type 4 (Protein N22.1) (Tctex-2-beta) (Tctex1 domain-containing protein 4) PROTEIN FAMILY: Dynein light chain Tctex-type family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the dynein light chain Tctex-type family. KEYWORDS: Cell projection;Cilium;Cytoplasm;Cytoplasmic vesicle;Cytoskeleton;Flagellum;Nucleus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cell projection, cilium, flagellum Cytoplasmic vesicle, secretory vesicle, acrosome Cytoplasm, cytoskeleton, cilium axoneme Cytoplasm Nucleus Cytoplasm, cytoskeleton, microtubule organizing center Note=Present along the entire length of the flagellum, including principal and endpiece, and more predominantly in the midpiece region. " Q5KTC7,"PROTEIN NAMES: N-acylethanolamine-hydrolyzing acid amidase (Acylsphingosine deacylase NAAA) (N-acylsphingosine amidohydrolase-like) (ASAH-like protein) [Cleaved into: N-acylethanolamine-hydrolyzing acid amidase subunit alpha; N-acylethanolamine-hydrolyzing acid amidase subunit beta] PROTEIN FAMILY: Acid ceramidase family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the acid ceramidase family. FUNCTION: Degrades bioactive fatty acid amides to their corresponding acids, with the following preference: N-palmitoylethanolamine > N-myristoylethanolamine > N-stearoylethanolamine > N-oleoylethanolamine > N-linoleoylethanolamine > N-arachidonoylethanolamine. PATHWAY: Lipid metabolism; fatty acid metabolism. KEYWORDS: Autocatalytic cleavage;Direct protein sequencing;Fatty acid metabolism;Glycoprotein;Hydrolase;Lipid degradation;Lipid metabolism;Lysosome;Membrane;Reference proteome;Signal;Zymogen SUBCELLULAR LOCATION: Lysosome Membrane ; Peripheral membrane protein " Q5LUF3,"PROTEIN NAMES: Propionyl-CoA carboxylase alpha chain ORGANISM: Ruegeria pomeroyi (strain ATCC 700808 / DSM 15171 / DSS-3) (Silicibacter pomeroyi) FUNCTION: This is one of the 2 subunits of the biotin-dependent propionyl-CoA carboxylase (PCC), the enzyme catalyzing the carboxylation of propionyl-CoA/propanoyl-CoA to D-methylmalonyl-CoA/(S)-methylmalonyl-CoA. Within the holoenzyme, the alpha subunit catalyzes the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain, while the beta subunit then tranfers the carboxyl group from carboxylated biotin to propionyl-CoA (Probable). PATHWAY: Metabolic intermediate metabolism; propanoyl-CoA degradation; succinyl-CoA from propanoyl-CoA: step 1/3. KEYWORDS: 3D-structure;ATP-binding;Biotin;Ligase;Lipid degradation;Lipid metabolism;Magnesium;Manganese;Metal-binding;Nucleotide-binding;Reference proteome " Q5LW89,"PROTEIN NAMES: Dimethylsulfonioproprionate lyase DddW (DMSP lyase) PROTEIN FAMILY: Non-heme iron-dependent dioxygenase family ORGANISM: Ruegeria pomeroyi (strain ATCC 700808 / DSM 15171 / DSS-3) (Silicibacter pomeroyi) SIMILARITY: Belongs to the non-heme iron-dependent dioxygenase family. FUNCTION: Able to cleave dimethylsulfonioproprionate (DMSP), releasing dimethyl sulfide (DMS) and acrylate. DMS is the principal form by which sulfur is transported from oceans to the atmosphere. KEYWORDS: Iron;Lyase;Metal-binding;Reference proteome " Q5M755,"PROTEIN NAMES: Fibrillin-5, chloroplastic (Plastid-lipid-associated protein 7) PROTEIN FAMILY: PAP/fibrillin family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the PAP/fibrillin family. FUNCTION: [Isoform 1]: Essential for plastoquinone-9 (PQ-9) biosynthesis. Interacts with the diphosphate synthases SPS1 and SPS2, and binds to the hydrophobic solanesyl moiety, which is generated by SPS1 and SPS2, in FBN5-SPS homodimeric complexes to stimulate the enzyme activity of SPS1 and SPS2. KEYWORDS: Alternative splicing;Chloroplast;Plastid;Reference proteome;Stress response;Transit peptide SUBCELLULAR LOCATION: [Isoform 1]: Plastid, chloroplast stroma " Q5M7Z0,"PROTEIN NAMES: E3 ubiquitin-protein ligase RNFT1 (Protein PTD016) (RING finger and transmembrane domain-containing protein 1) ORGANISM: Homo sapiens (Human) FUNCTION: E3 ubiquitin-protein ligase that acts in the endoplasmic reticulum (ER)-associated degradation (ERAD) pathway, which targets misfolded proteins that accumulate in the endoplasmic reticulum (ER) for ubiquitination and subsequent proteasome-mediated degradation. Protects cells from ER stress-induced apoptosis. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Alternative splicing;Endoplasmic reticulum;Membrane;Metal-binding;Reference proteome;Transferase;Transmembrane;Transmembrane helix;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein MISCELLANEOUS: [Isoform 3]: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay." Q5MJ70,"PROTEIN NAMES: Speedy protein A (Rapid inducer of G2/M progression in oocytes A) (RINGO A) (hSpy/Ringo A) (Speedy-1) (Spy1) PROTEIN FAMILY: Speedy/Ringo family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the Speedy/Ringo family. FUNCTION: Regulates the G1/S phase transition of the cell cycle by binding and activating CDK1 and CDK2. Contributes to CDK2 activation without promoting CDK2 phosphorylation, by inducing a conformation change of the CDK2 T-loop that obstructs the substrate-binding cleft prior to kinase activation. Mediates cell survival during the DNA damage process through activation of CDK2. KEYWORDS: 3D-structure;Alternative splicing;Cell cycle;Developmental protein;DNA damage;Nucleus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Nucleus " Q5MNZ6,"PROTEIN NAMES: WD repeat domain phosphoinositide-interacting protein 3 (WIPI-3) (WD repeat-containing protein 45-like) (WDR45-like protein) (WD repeat-containing protein 45B) (WIPI49-like protein) PROTEIN FAMILY: WD repeat PROPPIN family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the WD repeat PROPPIN family. FUNCTION: Component of the autophagy machinery that controls the major intracellular degradation process by which cytoplasmic materials are packaged into autophagosomes and delivered to lysosomes for degradation. Binds phosphatidylinositol 3-phosphate (PtdIns3P), and other phosphoinositides including PtdIns(3,5)P2, forming on membranes of the endoplasmic reticulum upon activation of the upstream ULK1 and PI3 kinases and is recruited at phagophore assembly sites where it regulates the elongation of nascent phagophores downstream of WIPI2. In the cellular response to starvation, may also function together with the TSC1-TSC2 complex and RB1CC1 in the inhibition of the mTORC1 signaling pathway. KEYWORDS: 3D-structure;Autophagy;Disease variant;Epilepsy;Lipid-binding;Lysosome;Reference proteome;Repeat;WD repeat SUBCELLULAR LOCATION: Preautophagosomal structure Lysosome " Q5NCC3,"PROTEIN NAMES: E3 ubiquitin-protein ligase TRIM41 (Tripartite motif-containing protein 41) PROTEIN FAMILY: TRIM/RBCC family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the TRIM/RBCC family. FUNCTION: E3 ligase that plays essential roles in innate antiviral response. Directly binds to influenza A virus or vesicular stomatitis virus nucleoproteins and targets them for ubiquitination and proteasomal degradation, thereby limiting viral infections (By similarity). Activates the innate antiviral response by catalyzing monoubiquitination of CGAS, thereby activating CGAS (By similarity). Also involved in innate antiviral response by mediating 'Lys-63'-linked polyubiquitylation of BCL10 which in turn hubs NEMO for activation of NF-kappa-B and IRF3 pathways. Catalyzes the ubiquitin-mediated degradation of other substrates including protein kinase C, ZSCAN21 or TOP3B suggesting additional roles besides its function in immune response. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Coiled coil;Cytoplasm;Isopeptide bond;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Transferase;Ubl conjugation;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cytoplasm Nucleus " Q5NCP0,"PROTEIN NAMES: E3 ubiquitin-protein ligase RNF43 (RING finger protein 43) (RING-type E3 ubiquitin transferase RNF43) PROTEIN FAMILY: ZNRF3 family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the ZNRF3 family. FUNCTION: E3 ubiquitin-protein ligase that acts as a negative regulator of the Wnt signaling pathway by mediating the ubiquitination, endocytosis and subsequent degradation of Wnt receptor complex components Frizzled. Acts on both canonical and non-canonical Wnt signaling pathway. Along with RSPO2 and ZNRF3, constitutes a master switch that governs limb specification (By similarity). PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Alternative splicing;Cell membrane;Developmental protein;Disulfide bond;Endoplasmic reticulum;Glycoprotein;Membrane;Metal-binding;Nucleus;Reference proteome;Signal;Transferase;Transmembrane;Transmembrane helix;Ubl conjugation;Ubl conjugation pathway;Wnt signaling pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein Endoplasmic reticulum membrane ; Single-pass type I membrane protein Nucleus envelope Note=May be secreted. " Q5NUA6,"PROTEIN NAMES: Nuclear factor erythroid 2-related factor 2 (NF-E2-related factor 2) (NFE2-related factor 2) (Nuclear factor, erythroid derived 2, like 2) PROTEIN FAMILY: BZIP family, CNC subfamily ORGANISM: Bos taurus (Bovine) SIMILARITY: Belongs to the bZIP family. CNC subfamily. FUNCTION: Transcription factor that plays a key role in the response to oxidative stress: binds to antioxidant response (ARE) elements present in the promoter region of many cytoprotective genes, such as phase 2 detoxifying enzymes, and promotes their expression, thereby neutralizing reactive electrophiles. In normal conditions, ubiquitinated and degraded in the cytoplasm by the BCR(KEAP1) complex. In response to oxidative stress, electrophile metabolites inhibit activity of the BCR(KEAP1) complex, promoting nuclear accumulation of NFE2L2/NRF2, heterodimerization with one of the small Maf proteins and binding to ARE elements of cytoprotective target genes. The NFE2L2/NRF2 pathway is also activated in response to selective autophagy: autophagy promotes interaction between KEAP1 and SQSTM1/p62 and subsequent inactivation of the BCR(KEAP1) complex, leading to NFE2L2/NRF2 nuclear accumulation and expression of cytoprotective genes (By similarity). May also be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region (By similarity). Plays also an important role in the regulation of the innate immune response. It is a critical regulator of the innate immune response and survival during sepsis by maintaining redox homeostasis and restraint of the dysregulation of pro-inflammatory signaling pathways like MyD88-dependent and -independent and TNF-alpha signaling. Suppresses macrophage inflammatory response by blocking pro-inflammatory cytokine transcription and the induction of IL6. Binds to the proximity of pro-inflammatory genes in macrophages and inhibits RNA Pol II recruitment. The inhibition is independent of the Nrf2-binding motif and reactive oxygen species level (By similarity). Represses antiviral cytosolic DNA sensing by suppressing the expression of the adapter protein STING1 and decreasing responsiveness to STING1 agonists while increasing susceptibility to infection with DNA viruses (By similarity). KEYWORDS: Acetylation;Activator;Cytoplasm;DNA-binding;Glycation;Glycoprotein;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation;Ubl conjugation SUBCELLULAR LOCATION: Cytoplasm, cytosol Nucleus Note=Cytosolic under unstressed conditions: ubiquitinated and degraded by the BCR(KEAP1) E3 ubiquitin ligase complex. Translocates into the nucleus upon induction by electrophilic agents that inactivate the BCR(KEAP1) E3 ubiquitin ligase complex. " Q5P5G5,PROTEIN NAMES: Acetophenone carboxylase delta subunit (Acetophenone carboxylase 75 kDa subunit) PROTEIN FAMILY: Oxoprolinase family ORGANISM: Aromatoleum aromaticum (strain EbN1) (Azoarcus sp. (strain EbN1)) SIMILARITY: Belongs to the oxoprolinase family. FUNCTION: Catalyzes the carboxylation of acetophenone to form 3-oxo-3-phenylpropanoate (benzoylacetate) in the anaerobic catabolism of ethylbenzene. Also carboxylates propiophenone at the same rate and 4-acetyl-pyridine at lower rates. KEYWORDS: 3D-structure;ATP-binding;Cytoplasm;Direct protein sequencing;Ligase;Nucleotide-binding;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Q5P5I4,PROTEIN NAMES: (S)-1-Phenylethanol dehydrogenase PROTEIN FAMILY: Short-chain dehydrogenases/reductases (SDR) family ORGANISM: Aromatoleum aromaticum (strain EbN1) (Azoarcus sp. (strain EbN1)) SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FUNCTION: Catalyzes the NAD-dependent stereospecific oxidation of (S)-1-phenylethanol to acetophenone in the degradation of ethylbenzene. KEYWORDS: 3D-structure;Direct protein sequencing;NAD;Nucleotide-binding;Oxidoreductase;Reference proteome Q5PQ50,"PROTEIN NAMES: Acyl-coenzyme A diphosphatase NUDT19 (Nucleoside diphosphate-linked moiety X motif 19) (Nudix motif 19) PROTEIN FAMILY: Nudix hydrolase family ORGANISM: Xenopus laevis (African clawed frog) SIMILARITY: Belongs to the Nudix hydrolase family. FUNCTION: Fatty acyl-coenzyme A (CoA) diphosphatase that hydrolyzes fatty acyl-CoA to yield acyl-4'-phosphopantetheine and adenosine 3',5'-bisphosphate (By similarity). Mediates the hydrolysis of a wide range of CoA esters, including choloyl-CoA and branched-chain fatty-acyl-CoA esters and at low substrate concentrations medium and long-chain fatty-acyl-CoA esters are the primary substrates (By similarity). Highest activity seen with medium-chain acyl-CoA esters and higher rates of activity seen with the unsaturated acyl-CoA esters compared with the saturated esters (By similarity). Exhibits decapping activity towards dpCoA-capped RNAs in vitro (By similarity). KEYWORDS: Hydrolase;Magnesium;Manganese;Metal-binding;Peroxisome;Reference proteome SUBCELLULAR LOCATION: Peroxisome " Q5PQV5,PROTEIN NAMES: Trophoblast glycoprotein (5T4 oncofetal trophoblast glycoprotein) (5T4 oncotrophoblast glycoprotein) (Wnt-activated inhibitory factor 1) (WAIF1) ORGANISM: Rattus norvegicus (Rat) FUNCTION: May function as an inhibitor of Wnt/beta-catenin signaling by indirectly interacting with LRP6 and blocking Wnt3a-dependent LRP6 internalization. KEYWORDS: Cell membrane;Disulfide bond;Glycoprotein;Leucine-rich repeat;Membrane;Phosphoprotein;Reference proteome;Repeat;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein Q5Q0E6,"PROTEIN NAMES: Type 2 DNA topoisomerase 6 subunit B-like (Meiotic topoisomerase VIB-like) (AtMTOPVIB) PROTEIN FAMILY: TOP6B-like family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the TOP6B-like family. FUNCTION: Component of a topoisomerase 6 complex specifically required for meiotic recombination. Together with SPO11 (SPO11-1 and SPO11-2), mediates DNA cleavage that forms the double-strand breaks (DSB) that initiate meiotic recombination. The complex promotes relaxation of negative and positive supercoiled DNA and DNA decatenation through cleavage and ligation cycles. KEYWORDS: Alternative splicing;Chromosome;Cytoplasm;Meiosis;Nucleus;Reference proteome SUBCELLULAR LOCATION: Chromosome Nucleus Cytoplasm Note=Localizes to meiotic chromosomes from leptotene to pachytene. Mostly present in the nucleus, but also present in the cytoplasm. " Q5QJ60,PROTEIN NAMES: Berberine bridge enzyme-like Cyn d 4 (60 kDa pollen antigen) (BG60) (Cyn d BG60) (FAD-linked oxidoreductase BG60) (Pollen isoallergen BG60) (allergen Cyn d 4) PROTEIN FAMILY: Oxygen-dependent FAD-linked oxidoreductase family ORGANISM: Cynodon dactylon (Bermuda grass) (Panicum dactylon) SIMILARITY: Belongs to the oxygen-dependent FAD-linked oxidoreductase family. KEYWORDS: 3D-structure;Allergen;Direct protein sequencing;Disulfide bond;FAD;Flavoprotein;Glycoprotein;Nucleotide-binding;Oxidoreductase;Signal Q5QJU3,"PROTEIN NAMES: Alkaline ceramidase 2 (AlkCDase 2) (Alkaline CDase 2) (haCER2) (Acylsphingosine deacylase 3-like) (N-acylsphingosine amidohydrolase 3-like) PROTEIN FAMILY: Alkaline ceramidase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the alkaline ceramidase family. FUNCTION: Golgi ceramidase that catalyzes the hydrolysis of ceramides into sphingoid bases like sphingosine and free fatty acids at alkaline pH. Ceramides, sphingosine, and its phosphorylated form sphingosine-1-phosphate are bioactive lipids that mediate cellular signaling pathways regulating several biological processes including cell proliferation, apoptosis and differentiation. Has a better catalytic efficiency towards unsaturated long-chain ceramides, including C18:1-, C20:1- and C24:1-ceramides. Saturated long-chain ceramides and unsaturated very long-chain ceramides are also good substrates, whereas saturated very long-chain ceramides and short-chain ceramides are poor substrates. Also hydrolyzes dihydroceramides to produce dihydrosphingosine. It is the ceramidase that controls the levels of circulating sphingosine-1-phosphate and dihydrosphingosine-1-phosphate in plasma through their production by hematopoietic cells (By similarity). Regulates cell proliferation, autophagy and apoptosis by the production of sphingosine and sphingosine-1-phosphate. As part of a p53/TP53-dependent pathway, promotes for instance autophagy and apoptosis in response to DNA damage. Through the production of sphingosine, may also regulate the function of the Golgi complex and regulate the glycosylation of proteins. PATHWAY: Lipid metabolism; sphingolipid metabolism. KEYWORDS: Alternative splicing;Calcium;Glycoprotein;Golgi apparatus;Hydrolase;Lipid metabolism;Membrane;Metal-binding;Reference proteome;Sphingolipid metabolism;Transmembrane;Transmembrane helix;Zinc SUBCELLULAR LOCATION: Golgi apparatus membrane ; Multi-pass membrane protein " Q5QM84,"PROTEIN NAMES: Two pore calcium channel protein 1 (OsTPC1) (Voltage-dependent calcium channel protein TPC1) PROTEIN FAMILY: Calcium channel alpha-1 subunit family, Two pore calcium channel subfamily ORGANISM: Oryza sativa subsp. japonica (Rice) SIMILARITY: Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. Two pore calcium channel subfamily. FUNCTION: May function as one of the major voltage-gated Ca(2+) channel (VDCC) across the plasma membrane. May be involved in the regulation of cytosolic Ca(2+) and in growth and development. Acts as the major ROS-responsive Ca(2+) channel and is the possible target of Al-dependent inhibition. Determines sensitivity to T.viride xylanase elicitor. Plays a regulatory role in elicitor-induced defense responses and hypersensitive cell death. KEYWORDS: Calcium;Calcium channel;Calcium transport;Glycoprotein;Ion channel;Ion transport;Membrane;Plant defense;Reference proteome;Repeat;Transmembrane;Transmembrane helix;Transport;Voltage-gated channel SUBCELLULAR LOCATION: Membrane ; Multi-pass membrane protein MISCELLANEOUS: Rescues the Ca(2+) uptake activity in yeast mutant cch1. Plants overexpressing TPC1 display reduced growth rate and dwarf phenotype, and seedlings show greening of roots under light conditions. The highest overexpressor line shows most severe phenotype including a death symptom, possibly due to Ca(2+) accumulation to toxic level." Q5R328,"PROTEIN NAMES: R-spondin-3 (Cabriolet) (Roof plate-specific spondin-3) PROTEIN FAMILY: R-spondin family ORGANISM: Danio rerio (Zebrafish) (Brachydanio rerio) SIMILARITY: Belongs to the R-spondin family. FUNCTION: Activator of the canonical Wnt signaling pathway by acting as a ligand for lgr4-6 receptors, which acts as a key regulator of angiogenesis. Upon binding to lgr4-6 (lgr4, lgr5 or lgr6), lgr4-6 associate with phosphorylated lrp6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Acts both in the canonical. Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway. Acts as a key regulator of angiogenesis by controlling vascular stability and pruning: acts by activating the non-canonical Wnt signaling pathway in endothelial cells (By similarity). Can also amplify Wnt signaling pathway independently of LGR4-6 receptors, possibly by acting as a direct antagonistic ligand to RNF43 and ZNRF3 (By similarity). KEYWORDS: Disulfide bond;Glycoprotein;Heparin-binding;Reference proteome;Repeat;Secreted;Sensory transduction;Signal;Wnt signaling pathway SUBCELLULAR LOCATION: Secreted " Q5R4L1,"PROTEIN NAMES: Serine/threonine-protein kinase PLK2 (Polo-like kinase 2) (PLK-2) PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family, CDC5/Polo subfamily ORGANISM: Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii) SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily. FUNCTION: Tumor suppressor serine/threonine-protein kinase involved in synaptic plasticity, centriole duplication and G1/S phase transition. Polo-like kinases act by binding and phosphorylating proteins that are already phosphorylated on a specific motif recognized by the POLO box domains. Phosphorylates CENPJ, NPM1, RAPGEF2, RASGRF1, SNCA, SIPA1L1 and SYNGAP1. Plays a key role in synaptic plasticity and memory by regulating the Ras and Rap protein signaling: required for overactivity-dependent spine remodeling by phosphorylating the Ras activator RASGRF1 and the Rap inhibitor SIPA1L1 leading to their degradation by the proteasome. Conversely, phosphorylates the Rap activator RAPGEF2 and the Ras inhibitor SYNGAP1, promoting their activity. Also regulates synaptic plasticity independently of kinase activity, via its interaction with NSF that disrupts the interaction between NSF and the GRIA2 subunit of AMPARs, leading to a rapid rundown of AMPAR-mediated current that occludes long term depression. Required for procentriole formation and centriole duplication by phosphorylating CENPJ and NPM1, respectively. Its induction by p53/TP53 suggests that it may participate in the mitotic checkpoint following stress (By similarity). KEYWORDS: ATP-binding;Cell projection;Cytoplasm;Cytoskeleton;Kinase;Nucleotide-binding;Phosphoprotein;Reference proteome;Repeat;Serine/threonine-protein kinase;Transferase;Tumor suppressor SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole Cell projection, dendrite Note=Localizes to centrosomes during early G1 phase where it only associates to the mother centriole and then distributes equally to both mother and daughter centrioles at the onset of S phase. " Q5RCW2,"PROTEIN NAMES: Polyribonucleotide nucleotidyltransferase 1, mitochondrial (Polynucleotide phosphorylase 1) (PNPase 1) PROTEIN FAMILY: Polyribonucleotide nucleotidyltransferase family ORGANISM: Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii) SIMILARITY: Belongs to the polyribonucleotide nucleotidyltransferase family. FUNCTION: RNA-binding protein implicated in numerous RNA metabolic processes. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'-to-5' direction. Mitochondrial intermembrane factor with RNA-processing exoribonulease activity. Component of the mitochondrial degradosome (mtEXO) complex, that degrades 3' overhang double-stranded RNA with a 3'-to-5' directionality in an ATP-dependent manner. Involved in the degradation of non-coding mitochondrial transcripts (MT-ncRNA) and tRNA-like molecules (By similarity). Required for correct processing and polyadenylation of mitochondrial mRNAs. Plays a role as a cytoplasmic RNA import factor that mediates the translocation of small RNA components like the 5S RNA, the RNA subunit of ribonuclease P and the mitochondrial RNA-processing (MRP) RNA, into the mitochondrial matrix. Plays a role in mitochondrial morphogenesis and respiration; regulates the expression of the electron transport chain (ETC) components at the mRNA and protein levels. In the cytoplasm, shows a 3'-to-5' exoribonuclease mediating mRNA degradation activity; degrades c-myc mRNA upon treatment with IFNB1/IFN-beta, resulting in a growth arrest in melanoma cells. Regulates the stability of specific mature miRNAs in melanoma cells; specifically and selectively degrades miR-221, preferentially. Also plays a role in RNA cell surveillance by cleaning up oxidized RNAs. Binds to the RNA subunit of ribonuclease P, MRP RNA and miR-221 microRNA (By similarity). KEYWORDS: Acetylation;Cytoplasm;Exonuclease;Hydrolase;Membrane;Mitochondrion;mRNA processing;Nuclease;Nucleotidyltransferase;Phosphoprotein;Reference proteome;RNA-binding;Transferase;Transit peptide;Transport SUBCELLULAR LOCATION: Cytoplasm Mitochondrion matrix Mitochondrion intermembrane space ; Peripheral membrane protein " Q5RED8,"PROTEIN NAMES: Ubiquitin carboxyl-terminal hydrolase CYLD (Deubiquitinating enzyme CYLD) (Ubiquitin thioesterase CYLD) (Ubiquitin-specific-processing protease CYLD) PROTEIN FAMILY: Peptidase C19 family ORGANISM: Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii) SIMILARITY: Belongs to the peptidase C19 family. FUNCTION: Deubiquitinase that specifically cleaves 'Lys-63'- and linear 'Met-1'-linked polyubiquitin chains and is involved in NF-kappa-B activation and TNF-alpha-induced necroptosis. Negatively regulates NF-kappa-B activation by deubiquitinating upstream signaling factors. Contributes to the regulation of cell survival, proliferation and differentiation via its effects on NF-kappa-B activation. Negative regulator of Wnt signaling. Inhibits HDAC6 and thereby promotes acetylation of alpha-tubulin and stabilization of microtubules. Plays a role in the regulation of microtubule dynamics, and thereby contributes to the regulation of cell proliferation, cell polarization, cell migration, and angiogenesis. Required for normal cell cycle progress and normal cytokinesis. Inhibits nuclear translocation of NF-kappa-B. Plays a role in the regulation of inflammation and the innate immune response, via its effects on NF-kappa-B activation (By similarity). Dispensable for the maturation of intrathymic natural killer cells, but required for the continued survival of immature natural killer cells. Negatively regulates TNFRSF11A signaling and osteoclastogenesis. Involved in the regulation of ciliogenesis, allowing ciliary basal bodies to migrate and dock to the plasma membrane; this process does not depend on NF-kappa-B activation (By similarity). Ability to remove linear ('Met-1'-linked) polyubiquitin chains regulates innate immunity and TNF-alpha-induced necroptosis: recruited to the LUBAC complex via interaction with SPATA2 and restricts linear polyubiquitin formation on target proteins. Regulates innate immunity by restricting linear polyubiquitin formation on RIPK2 in response to NOD2 stimulation (By similarity). Involved in TNF-alpha-induced necroptosis by removing linear ('Met-1'-linked) polyubiquitin chains from RIPK1, thereby regulating the kinase activity of RIPK1 (By similarity). Negatively regulates intestinal inflammation by removing 'Lys-63' linked polyubiquitin chain of NLRP6, thereby reducing the interaction between NLRP6 and PYCARD/ASC and formation of the NLRP6 inflammasome (By similarity). Removes 'Lys-63' linked polyubiquitin chain of MAP3K7, which inhibits phosphorylation and blocks downstream activation of the JNK-p38 kinase cascades (By similarity). Removes also 'Lys-63'-linked polyubiquitin chains of MAP3K1 and MA3P3K3, which inhibit their interaction with MAP2K1 and MAP2K2 (By similarity). KEYWORDS: Cell membrane;Cell projection;Cytoplasm;Cytoskeleton;Hydrolase;Immunity;Innate immunity;Membrane;Metal-binding;Microtubule;Phosphoprotein;Protease;Reference proteome;Repeat;Thiol protease;Ubl conjugation;Ubl conjugation pathway;Wnt signaling pathway;Zinc SUBCELLULAR LOCATION: Cytoplasm. Cytoplasm, perinuclear region. Cytoplasm, cytoskeleton. Cell membrane ; Peripheral membrane protein ; Cytoplasmic side Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Cytoplasm, cytoskeleton, spindle Cytoplasm, cytoskeleton, cilium basal body Note=Detected at the microtubule cytoskeleton during interphase (By similarity). Detected at the midbody during telophase (By similarity). During metaphase, it remains localized to the centrosome but is also present along the spindle (By similarity). " Q5RFL1,PROTEIN NAMES: Tripeptidyl-peptidase 1 (TPP-1) (Tripeptidyl aminopeptidase) (Tripeptidyl-peptidase I) (TPP-I) ORGANISM: Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii) FUNCTION: Lysosomal serine protease with tripeptidyl-peptidase I activity. May act as a non-specific lysosomal peptidase which generates tripeptides from the breakdown products produced by lysosomal proteinases. Requires substrates with an unsubstituted N-terminus (By similarity). KEYWORDS: Autocatalytic cleavage;Calcium;Disulfide bond;Glycoprotein;Hydrolase;Lysosome;Metal-binding;Protease;Reference proteome;Serine protease;Signal;Zymogen SUBCELLULAR LOCATION: Lysosome Melanosome Q5RGQ2,"PROTEIN NAMES: Protein arginine N-methyltransferase 8-B (Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 4) (zfL3) PROTEIN FAMILY: Class I-like SAM-binding methyltransferase superfamily, Protein arginine N-methyltransferase family, PRMT8 subfamily ORGANISM: Danio rerio (Zebrafish) (Brachydanio rerio) SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N-methyltransferase family. PRMT8 subfamily. FUNCTION: S-adenosyl-L-methionine-dependent and membrane-associated arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and asymmetrical dimethylarginine (aDMA). KEYWORDS: Cell membrane;Lipoprotein;Membrane;Methylation;Methyltransferase;Myristate;Reference proteome;Repeat;S-adenosyl-L-methionine;Transferase SUBCELLULAR LOCATION: Cell membrane ; Lipid-anchor ; Cytoplasmic side " Q5RLZ1,"PROTEIN NAMES: Halolysin-like extracellular serine protease Nep (Subtilisin-like protease Nep) PROTEIN FAMILY: Peptidase S8 family ORGANISM: Natrialba magadii SIMILARITY: Belongs to the peptidase S8 family. FUNCTION: Serine protease that hydrolyzes large proteins such as casein and gelatin. Cleaves preferentially at the carboxyl terminus of Phe, Tyr or Leu. Is also able to catalyze peptide synthesis under different salt concentrations in the presence of dimethyl sulfoxide (DMSO). KEYWORDS: Direct protein sequencing;Hydrolase;Protease;Secreted;Serine protease;Signal;Zymogen SUBCELLULAR LOCATION: Secreted " Q5S3I3,"PROTEIN NAMES: Dicamba O-demethylase, oxygenase component (Dicamba monooxygenase) (DMO) (Three-component Rieske non-heme iron oxygenase system) ORGANISM: Stenotrophomonas maltophilia (Pseudomonas maltophilia) (Xanthomonas maltophilia) FUNCTION: Component of the dicamba O-demethylase multicomponent enzyme system involved in the degradation of the herbicide dicamba. In vitro, catalyzes the O-demethylation of 2-methoxy-3,6-dichlorobenzoic acid (dicamba) to yield 3,6-dichlorosalicylic acid (DCSA) via an exocyclic monooxygenation. KEYWORDS: 2Fe-2S;3D-structure;Aromatic hydrocarbons catabolism;Direct protein sequencing;Iron;Iron-sulfur;Metal-binding;Monooxygenase;Oxidoreductase " Q5SH28,"PROTEIN NAMES: 1,4-alpha-glucan branching enzyme TTHA1902 (1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase) (Alpha-(1->4)-glucan branching enzyme) (Branching enzyme) (BE) PROTEIN FAMILY: Glycosyl hydrolase 57 family ORGANISM: Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) SIMILARITY: Belongs to the glycosyl hydrolase 57 family. FUNCTION: Catalyzes the formation of branch points in alpha-glucans by cleavage of an alpha-1,4 glycosidic bond and subsequent transfer of the cleaved-off oligosaccharide to a new alpha-1,6 position. The branch chain-length distribution of the reaction products shows degree of polymerization (DP) of 3 to 13, with two local maxima at DP 7 and DP 11. Exhibits an alpha-retaining catalytic mechanism. Is involved in glycogen biosynthesis. Shows a secondary activity, i.e. the hydrolysis of the substrate, being 4% of the total activity. Can use amylose as substrate but not alpha-1,4-linked oligosaccharides of 2-7 glucose residues, beta-cyclodextrin, 6-O-glucosyl-beta-cyclodextrin and 6-O-maltosyl-beta-cyclodextrin. Is not able to branch amylopectin further, it only hydrolyzes amylopectin. Thus, displays preference for linear and long substrates (amylose) over branched structures (amylopectin). PATHWAY: Glycan biosynthesis; glycogen biosynthesis. KEYWORDS: 3D-structure;Carbohydrate metabolism;Glycogen biosynthesis;Glycogen metabolism;Glycosyltransferase;Reference proteome;Transferase " Q5SM25,"PROTEIN NAMES: Bifunctional oligoribonuclease and PAP phosphatase NrnA (3'(2'),5'-bisphosphate nucleotidase) (3'-phosphoadenosine 5'-phosphate phosphatase) (PAP phosphatase) PROTEIN FAMILY: NrnA oligoribonuclease family ORGANISM: Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) SIMILARITY: Belongs to the NrnA oligoribonuclease family. FUNCTION: Bifunctional enzyme which has both oligoribonuclease and pAp-phosphatase activities. Degrades short RNA and DNA oligonucleotides with a length of up to 33 nucleotides, although the enzyme is most active on shorter substrates, in a 5' to 3' direction. Converts 3'(2')-phosphoadenosine 5'-phosphate (PAP) to AMP, has very low activity on cAMP and cGMP. KEYWORDS: Exonuclease;Hydrolase;Nuclease;Reference proteome " Q5SMC7,"PROTEIN NAMES: tRNA-dihydrouridine(20/20a) synthase (DusA-like U20-specific dihydrouridine synthase) (U20-specific Dus) PROTEIN FAMILY: Dus family, DusA subfamily ORGANISM: Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) SIMILARITY: Belongs to the Dus family. DusA subfamily. FUNCTION: Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines. Specifically modifies U20 and U20a in tRNAs. KEYWORDS: 3D-structure;Flavoprotein;FMN;NADP;Nucleotide-binding;Oxidoreductase;Reference proteome;RNA-binding;tRNA processing;tRNA-binding " Q5SMG8,"PROTEIN NAMES: Magnesium transporter MgtE PROTEIN FAMILY: SLC41A transporter family ORGANISM: Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) SIMILARITY: Belongs to the SLC41A transporter family. FUNCTION: Highly selective magnesium channel that plays an important role in Mg(2+) homeostasis. Functions as a Mg(2+)-dependent gating channel. Exhibits low activity with cobalt, suggesting that it might also be involved in the uptake of Co(2+) as a micronutrient. Also exhibits low activity with Ca(2+), but it shows almost no activity with Mn(2+). KEYWORDS: 3D-structure;Calcium;CBS domain;Cell inner membrane;Cell membrane;Magnesium;Manganese;Membrane;Metal-binding;Reference proteome;Repeat;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell inner membrane ; Multi-pass membrane protein " Q5SPR8,"PROTEIN NAMES: N-acetyltransferase ESCO2 (Establishment of cohesion 1 homolog 2) PROTEIN FAMILY: Acetyltransferase family, ECO subfamily ORGANISM: Danio rerio (Zebrafish) (Brachydanio rerio) SIMILARITY: Belongs to the acetyltransferase family. ECO subfamily. FUNCTION: Acetyltransferase required for the establishment of sister chromatid cohesion. Couples the processes of cohesion and DNA replication to ensure that only sister chromatids become paired together (By similarity). Essential for early development. KEYWORDS: Acyltransferase;Cell cycle;Chromosome;Metal-binding;Nucleus;Reference proteome;Transferase;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Chromosome " Q5SQN1,"PROTEIN NAMES: Synaptosomal-associated protein 47 (SNAP-47) (Epididymis luminal protein 170) (Synaptosomal-associated 47 kDa protein) PROTEIN FAMILY: SVAP1 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the SVAP1 family. FUNCTION: Plays a role in intracellular membrane fusion. KEYWORDS: Alternative splicing;Coiled coil;Cytoplasm;Membrane;Reference proteome;Repeat SUBCELLULAR LOCATION: Endomembrane system Cytoplasm, perinuclear region Note=Appears to be exclusively membrane-bound. " Q5SRR4,PROTEIN NAMES: Lymphocyte antigen 6 complex locus protein G5c ORGANISM: Homo sapiens (Human) FUNCTION: May have a role in hematopoietic cell differentiation. KEYWORDS: Alternative splicing;Disulfide bond;Glycoprotein;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted Q5SSG5,"PROTEIN NAMES: Ras-like protein family member 10B PROTEIN FAMILY: Small GTPase superfamily, Ras family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the small GTPase superfamily. Ras family. FUNCTION: May facilitate the release of atrial natriuretic peptide by cardiomyocytes and hence play a role in the regulation of arterial pressure. KEYWORDS: Cell membrane;GTP-binding;Hydrolase;Lipoprotein;Membrane;Methylation;Nucleotide-binding;Prenylation;Reference proteome SUBCELLULAR LOCATION: Cell membrane ; Lipid-anchor ; Cytoplasmic side " Q5SVD0,"PROTEIN NAMES: Refilin-B (Regulator of filamin protein B) (RefilinB) PROTEIN FAMILY: Refilin family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the Refilin family. FUNCTION: Involved in the regulation of the perinuclear actin network and nuclear shape through interaction with filamins. Plays an essential role in the formation of cartilaginous skeletal elements. KEYWORDS: Cytoplasm;Cytoskeleton;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton Note=Colocalizes with FLNA along actin bundle-like structures. " Q5SVQ0,"PROTEIN NAMES: Histone acetyltransferase KAT7 (Histone acetyltransferase binding to ORC1) (Lysine acetyltransferase 7) (MOZ, YBF2/SAS3, SAS2 and TIP60 protein 2) (MYST-2) PROTEIN FAMILY: MYST (SAS/MOZ) family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the MYST (SAS/MOZ) family. FUNCTION: Catalytic subunit of histone acetyltransferase HBO1 complexes, which specifically mediate acetylation of histone H3 at 'Lys-14' (H3K14ac), thereby regulating various processes, such as gene transcription, protein ubiquitination, immune regulation, stem cell pluripotent and self-renewal maintenance and embryonic development. Some complexes also catalyze acetylation of histone H4 at 'Lys-5', 'Lys-8' and 'Lys-12' (H4K5ac, H4K8ac and H4K12ac, respectively), regulating DNA replication initiation, regulating DNA replication initiation (By similarity). Specificity of the HBO1 complexes is determined by the scaffold subunit: complexes containing BRPF scaffold (BRPF1, BRD1/BRPF2 or BRPF3) direct KAT7/HBO1 specificity towards H3K14ac, while complexes containing JADE (JADE1, JADE2 and JADE3) scaffold direct KAT7/HBO1 specificity towards histone H4 (By similarity). H3K14ac promotes transcriptional elongation by facilitating the processivity of RNA polymerase II. Acts as a key regulator of hematopoiesis by forming a complex with BRD1/BRPF2, directing KAT7/HBO1 specificity towards H3K14ac and promoting erythroid differentiation (By similarity). H3K14ac is also required for T-cell development. KAT7/HBO1-mediated acetylation facilitates two consecutive steps, licensing and activation, in DNA replication initiation: H3K14ac facilitates the activation of replication origins, and histone H4 acetylation (H4K5ac, H4K8ac and H4K12ac) facilitates chromatin loading of MCM complexes, promoting DNA replication licensing (By similarity). Acts as a positive regulator of centromeric CENPA assembly: recruited to centromeres and mediates histone acetylation, thereby preventing centromere inactivation mediated by SUV39H1, possibly by increasing histone turnover/exchange (By similarity). Involved in nucleotide excision repair: phosphorylation by ATR in response to ultraviolet irradiation promotes its localization to DNA damage sites, where it mediates histone acetylation to facilitate recruitment of XPC at the damaged DNA sites (By similarity). Acts as an inhibitor of NF-kappa-B independently of its histone acetyltransferase activity (By similarity).; FUNCTION: Plays a central role in the maintenance of leukemia stem cells in acute myeloid leukemia (AML). Acts by mediating acetylation of histone H3 at 'Lys-14' (H3K14ac), thereby facilitating the processivity of RNA polymerase II to maintain the high expression of key genes, such as HOXA9 and HOXA10 that help to sustain the functional properties of leukemia stem cells. KEYWORDS: Acetylation;Acyltransferase;Alternative splicing;Centromere;Chromatin regulator;Chromosome;Cytoplasm;DNA damage;DNA repair;DNA replication;Isopeptide bond;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation;Transferase;Ubl conjugation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Chromosome Chromosome, centromere Cytoplasm, cytosol Note=Associates with replication origins specifically during the G1 phase of the cell cycle. Localizes to transcription start sites. Localizes to ultraviolet-induced DNA damage sites following phosphorylation by ATR. Localizes to centromeres in G1 phase. " Q5T0T0,"PROTEIN NAMES: E3 ubiquitin-protein ligase MARCHF8 (Cellular modulator of immune recognition) (c-MIR) (Membrane-associated RING finger protein 8) (Membrane-associated RING-CH protein VIII) (MARCH-VIII) (RING finger protein 178) (RING-type E3 ubiquitin transferase MARCHF8) ORGANISM: Homo sapiens (Human) FUNCTION: E3 ubiquitin-protein ligase that plays several important roles in innate immunity and adaptive immunity. Mediates ubiquitination of CD86 and MHC class II proteins, such as HLA-DR alpha and beta, and promotes their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. Possesses a very broad antiviral activity by specifically inactivating different viral fusion proteins. Targets and ubiquitinates cytoplasmic lysine residues of viral envelope glycoproteins with single transmembrane domains leading to their lysosomal degradation. Therefore, shows broad-spectrum inhibition against many viruses including retroviruses, rhabdoviruses, arenaviruses, sarbecoviruses or influenzaviruses. Strongly blocks human immunodeficiency virus type 1 envelope glycoprotein incorporation into virions by down-regulating its cell surface expression. Blocks also ebola virus glycoprotein/GP incorporation via surface down-regulation. Mediates 'Lys-63'-linked polyubiquitination of influenza M2 to target it to lysosome for degradation. Mediates the regulation of constitutive ubiquitination and trafficking of the viral restriction factor BST2 within the endocytic pathway. Plays a role in maintenance of immune tolerance to self by promoting the turnover and proteasomal degradation of PD-L1/CD274 via ubiquitination. Catalyzes the 'Lys-63'-linked polyubiquitylation of cGAS thereby inhibiting its DNA binding ability and impairing its antiviral innate immunity.; FUNCTION: (Microbial infection) Mediates 'Lys-63'-linked polyubiquitination of hepatitis C virus/HCV protein NS2 which allows its binding to HGS, an ESCRT-0 complex component, and this interaction is essential for HCV envelopment. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: 3D-structure;Adaptive immunity;Alternative splicing;Cytoplasmic vesicle;Endoplasmic reticulum;Endosome;Golgi apparatus;Immunity;Lysosome;Membrane;Metal-binding;Phosphoprotein;Reference proteome;Transferase;Transmembrane;Transmembrane helix;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Golgi apparatus membrane Endoplasmic reticulum membrane Cytoplasmic vesicle membrane ; Multi-pass membrane protein Lysosome membrane ; Multi-pass membrane protein Early endosome membrane ; Multi-pass membrane protein " Q5T124,"PROTEIN NAMES: UBX domain-containing protein 11 (Colorectal tumor-associated antigen COA-1) (Socius) (UBX domain-containing protein 5) ORGANISM: Homo sapiens (Human) FUNCTION: May be involved in the reorganization of actin cytoskeleton mediated by RND1, RND2 and RND3. Promotes RHOA activation mediated by GNA12 and GNA13 (By similarity). KEYWORDS: Alternative splicing;Coiled coil;Cytoplasm;Cytoskeleton;Reference proteome;Repeat SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton " Q5T2D3,"PROTEIN NAMES: OTU domain-containing protein 3 ORGANISM: Homo sapiens (Human) FUNCTION: Deubiquitinating enzyme that hydrolyzes 'Lys-6'- and 'Lys-11'-linked polyubiquitin. Also hydrolyzes heterotypic (mixed and branched) and homotypic chains. Important regulator of energy metabolism. Glucose and fatty acids trigger its nuclear translocation by CBP-dependent acetylation. In the nucleus, deubiquitinates and stabilizes the nuclear receptor PPARD regulating the expression of various genes involved in glucose and lipid metabolism and oxidative phosphorylation. Also acts as a negative regulator of the ribosome quality control (RQC) by mediating deubiquitination of 40S ribosomal proteins RPS10/eS10 and RPS20/uS10, thereby antagonizing ZNF598-mediated 40S ubiquitination. KEYWORDS: 3D-structure;Acetylation;Cytoplasm;Hydrolase;Nucleus;Phosphoprotein;Protease;Reference proteome;Thiol protease;Ubl conjugation pathway SUBCELLULAR LOCATION: Cytoplasm Nucleus Note=Glucose or fatty acid promote nuclear translocation upon acetylation. " Q5T4D3,"PROTEIN NAMES: Protein O-mannosyl-transferase TMTC4 (Transmembrane O-mannosyltransferase targeting cadherins 4) (Transmembrane and tetratricopeptide repeat-containing 4) PROTEIN FAMILY: TMTC family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the TMTC family. FUNCTION: Transfers mannosyl residues to the hydroxyl group of serine or threonine residues. The 4 members of the TMTC family are O-mannosyl-transferases dedicated primarily to the cadherin superfamily, each member seems to have a distinct role in decorating the cadherin domains with O-linked mannose glycans at specific regions. Also acts as O-mannosyl-transferase on other proteins such as PDIA3. PATHWAY: Protein modification; protein glycosylation. KEYWORDS: Alternative splicing;Endoplasmic reticulum;Glycoprotein;Membrane;Reference proteome;Repeat;TPR repeat;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Multi-pass membrane protein Endoplasmic reticulum " Q5T4F7,"PROTEIN NAMES: Secreted frizzled-related protein 5 (sFRP-5) (Frizzled-related protein 1b) (FRP-1b) (Secreted apoptosis-related protein 3) (SARP-3) PROTEIN FAMILY: Secreted frizzled-related protein (sFRP) family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the secreted frizzled-related protein (sFRP) family. FUNCTION: Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP5 may be involved in determining the polarity of photoreceptor, and perhaps, other cells in the retina. KEYWORDS: Developmental protein;Differentiation;Disulfide bond;Reference proteome;Secreted;Signal;Wnt signaling pathway SUBCELLULAR LOCATION: Secreted " Q5T5X7,"PROTEIN NAMES: BEN domain-containing protein 3 ORGANISM: Homo sapiens (Human) FUNCTION: Transcriptional repressor which associates with the NoRC (nucleolar remodeling complex) complex and plays a key role in repressing rDNA transcription. The sumoylated form modulates the stability of the NoRC complex component BAZ2A/TIP5 by controlling its USP21-mediated deubiquitination. Binds to unmethylated major satellite DNA and is involved in the recruitment of the Polycomb repressive complex 2 (PRC2) to major satellites (By similarity). Stimulates the ERCC6L translocase and ATPase activities. KEYWORDS: 3D-structure;Chromatin regulator;DNA-binding;Isopeptide bond;Nucleus;Phosphoprotein;Reference proteome;Repeat;Repressor;Transcription;Transcription regulation;Ubl conjugation SUBCELLULAR LOCATION: Nucleus Nucleus, nucleolus Note=In the nucleus, observed in heterochromatic foci containing CBX1, CBX3, CBX5 and histone H3 trimethylated at 'Lys-9'. Released from chromatin during decondensation. Association with heterochromatin does not depend on sumoylation. " Q5T601,"PROTEIN NAMES: Adhesion G-protein coupled receptor F1 (G protein-coupled receptor 110) (G protein-coupled receptor KPG_012) (G protein-coupled receptor PGR19) PROTEIN FAMILY: G-protein coupled receptor 2 family, Adhesion G-protein coupled receptor (ADGR) subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily. FUNCTION: Orphan receptor. KEYWORDS: 3D-structure;Alternative splicing;Cell membrane;Disulfide bond;G-protein coupled receptor;Glycoprotein;Membrane;Receptor;Reference proteome;Secreted;Signal;Transducer;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein.; SUBCELLULAR LOCATION: [Isoform 2]: Secreted " Q5T7W0,PROTEIN NAMES: Zinc finger protein 618 PROTEIN FAMILY: Krueppel C2H2-type zinc-finger protein family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein family. FUNCTION: Regulates UHRF2 function as a specific 5-hydroxymethylcytosine (5hmC) reader by regulating its chromatin localization. KEYWORDS: Acetylation;Alternative splicing;Chromosome;DNA-binding;Isopeptide bond;Metal-binding;Nucleus;Reference proteome;Repeat;Transcription;Transcription regulation;Ubl conjugation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Chromosome Note=Localizes at genomic loci that are enriched for 5-hydroxymethylcytosine (5hmC). Q5TBB1,"PROTEIN NAMES: Ribonuclease H2 subunit B (RNase H2 subunit B) (Aicardi-Goutieres syndrome 2 protein) (AGS2) (Deleted in lymphocytic leukemia 8) (Ribonuclease HI subunit B) PROTEIN FAMILY: RNase H2 subunit B family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the RNase H2 subunit B family. FUNCTION: Non catalytic subunit of RNase H2, an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. Participates in DNA replication, possibly by mediating the removal of lagging-strand Okazaki fragment RNA primers during DNA replication. Mediates the excision of single ribonucleotides from DNA:RNA duplexes. KEYWORDS: 3D-structure;Acetylation;Aicardi-Goutieres syndrome;Alternative splicing;Disease variant;Nucleus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Nucleus " Q5U349,"PROTEIN NAMES: Ubiquitin carboxyl-terminal hydrolase 2 (41 kDa ubiquitin-specific protease) (Deubiquitinating enzyme 2) (Ubiquitin thioesterase 2) (Ubiquitin-specific-processing protease 2) (Ubiquitin-specific-processing protease testis) (UBP-t) PROTEIN FAMILY: Peptidase C19 family, USP2 subfamily ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the peptidase C19 family. USP2 subfamily. FUNCTION: Hydrolase that deubiquitinates polyubiquitinated target proteins such as MDM2, MDM4 and CCND1 (By similarity). Isoform 1 and isoform 2 possess both ubiquitin-specific peptidase and isopeptidase activities. Deubiquitinates MDM2 without reversing MDM2-mediated p53/TP53 ubiquitination and thus indirectly promotes p53/TP53 degradation and limits p53 activity (By similarity). Has no deubiquitinase activity against p53/TP53 (By similarity). Prevents MDM2-mediated degradation of MDM4 (By similarity). Plays a role in the G1/S cell-cycle progression in normal and cancer cells (By similarity). Regulates the circadian clock by modulating its intrinsic circadian rhythm and its capacity to respond to external cues. Associates with clock proteins and deubiquitinates core clock component PER1 but does not affect its overall stability. Regulates the nucleocytoplasmic shuttling and nuclear retention of PER1 and its repressive role on the clock transcription factors CLOCK and BMAL1 (By similarity). Plays a role in the regulation of myogenic differentiation of embryonic muscle cells.; FUNCTION: [Isoform 2]: Circadian clock output effector that regulates Ca(2+) absorption in the small intestine. Probably functions by regulating protein levels of the membrane scaffold protein NHERF4 in a rhythmic manner, and is therefore likely to control Ca(2+) membrane permeability mediated by the Ca(2+) channel TRPV6 in the intestine. KEYWORDS: Alternative splicing;Biological rhythms;Cell cycle;Cytoplasm;Hydrolase;Membrane;Metal-binding;Myogenesis;Nucleus;Protease;Reference proteome;Thiol protease;Ubl conjugation pathway;Zinc SUBCELLULAR LOCATION: Cytoplasm.; SUBCELLULAR LOCATION: [Isoform 1]: Cytoplasm, perinuclear region Note=Localizes in the spermatid head in late-elongating spermatids in the thin area between the outer acrosomal membrane and the plasma membrane.; SUBCELLULAR LOCATION: [Isoform 2]: Nucleus Membrane ; Peripheral membrane protein Cytoplasm Note=Predominantly expressed at membranes. MISCELLANEOUS: [Isoform 1]: Stimulates both membrane fusion during myogenesis and accumulation of muscle-specific proteins.; MISCELLANEOUS: [Isoform 2]: Inhibits both membrane fusion during myogenesis and accumulation of muscle-specific proteins." Q5U5Q3,"PROTEIN NAMES: RNA-binding E3 ubiquitin-protein ligase MEX3C (RING finger and KH domain-containing protein 2) (RING finger protein 194) (RING-type E3 ubiquitin transferase MEX3C) ORGANISM: Homo sapiens (Human) FUNCTION: E3 ubiquitin ligase responsible for the post-transcriptional regulation of common HLA-A allotypes. Binds to the 3' UTR of HLA-A2 mRNA, and regulates its levels by promoting mRNA decay. RNA binding is sufficient to prevent translation, but ubiquitin ligase activity is required for mRNA degradation. KEYWORDS: 3D-structure;Cytoplasm;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Repeat;RNA-binding;Transferase;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cytoplasm Nucleus Note=Predominantly expressed in the cytoplasm and shuttles between the cytoplasm and the nucleus through the CRM1 export pathway. May act as suppressor of replication stress and chromosome missegregation. " Q5U780,"PROTEIN NAMES: Lipase (Lip 42) (Thermostable organic solvent tolerant lipase) (Triacylglycerol hydrolase) PROTEIN FAMILY: AB hydrolase superfamily ORGANISM: Bacillus sp SIMILARITY: Belongs to the AB hydrolase superfamily. FUNCTION: Triacylglycerol hydrolase that shows hydrolysis preference towards some of the natural oils such as olive, sunflower and corn oils. KEYWORDS: 3D-structure;Calcium;Hydrolase;Lipid degradation;Lipid metabolism;Metal-binding;Secreted;Signal SUBCELLULAR LOCATION: Secreted MISCELLANEOUS: Is stable in polar organic solvents such as DMSO, DMF, acetone, methanol, ethanol, heptanol and octanol, which could make it as a potential biocatalyst for the use in industrial biodiesel production." Q5UKY4,"PROTEIN NAMES: Cell surface glycoprotein CD200 receptor 3 (CD200 cell surface glycoprotein receptor-like 3) (CD200 receptor-like 3) (CD200 cell surface glycoprotein receptor-like b) (CD200RLb) (Cell surface glycoprotein OX2 receptor 3) PROTEIN FAMILY: CD200R family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the CD200R family. FUNCTION: According to previous research is a receptor for the CD200 cell surface glycoprotein. According to previous research is not a receptor for the CD200/OX2 cell surface glycoprotein. Isoform 1, isoform 2 and isoform 3 are involved in the recruitment or surface expression of the TYROBP receptor. Isoform 6, isoform 7 and isoform 8 are not involved in the recruitment or surface expression of the TYROBP receptor. KEYWORDS: Alternative splicing;Disulfide bond;Glycoprotein;Immunoglobulin domain;Membrane;Reference proteome;Repeat;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Single-pass type I membrane protein " Q5UW37,"PROTEIN NAMES: C-X-C motif chemokine 17 (6-Cys CXCL17) (13.6 kDa protein) (VEGF coregulated chemokine 1) [Cleaved into: 4-Cys CXCL17] PROTEIN FAMILY: Intercrine alpha (chemokine CxC) family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the intercrine alpha (chemokine CxC) family. FUNCTION: Chemokine that acts as a chemoattractant for monocytes, macrophages and dendritic cells. Plays a role in angiogenesis and possibly in the development of tumors. Acts as an anti-inflammatory in the stomach. May play a role in the innate defense against infections. Activates the C-X-C chemokine receptor GPR35 to induce a rapid and transient rise in the level of intracellular calcium ions. KEYWORDS: Angiogenesis;Chemotaxis;Cleavage on pair of basic residues;Developmental protein;Differentiation;Disulfide bond;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted MISCELLANEOUS: All the mice injected with NIH3T3 cells over-expressing Cxcl17 develop rapidly progressing tumors within 21 days." Q5V463,"PROTEIN NAMES: L-malyl-CoA/beta-methylmalyl-CoA lyase ((3S)-malyl-CoA/beta-methylmalyl-CoA lyase) PROTEIN FAMILY: HpcH/HpaI aldolase family ORGANISM: Haloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809) (Halobacterium marismortui) SIMILARITY: Belongs to the HpcH/HpaI aldolase family. FUNCTION: Involved in the methylaspartate cycle. Catalyzes the reversible cleavage of beta-methylmalyl-CoA to propionyl-CoA and glyoxylate, as well as the reversible cleavage of (S)-malyl-CoA to acetyl-CoA and glyoxylate. In addition, it has a small malyl-CoA thioesterase activity. It can also catalyze the cleavage of (S)-citramalyl-CoA to acetyl-CoA and pyruvate. KEYWORDS: Lyase;Magnesium;Manganese;Metal-binding;Reference proteome " Q5VW38,"PROTEIN NAMES: Protein GPR107 (Lung seven transmembrane receptor 1) PROTEIN FAMILY: LU7TM family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the LU7TM family. FUNCTION: Has been proposed to act as a receptor for neuronostatin, a peptide derived from the somatostatin/SST precursor. Involved in blood sugar regulation through the induction of glucagon in response to low glucose (By similarity).; FUNCTION: (Microbial infection) Required for intoxication by Pseudomonas aeruginosa exotoxin A and Campylobacter jejuni CDT. May contribute to the retrograde transport of bacterial toxins, including cholera toxin, from the trans-Golgi network to the endoplasmic reticulum. KEYWORDS: Alternative splicing;Cell membrane;Disulfide bond;Glycoprotein;Golgi apparatus;Membrane;Reference proteome;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Golgi apparatus, trans-Golgi network membrane " Q5W726,"PROTEIN NAMES: Gibberellin 2-beta-dioxygenase 1 (Gibberellin 2-beta-hydroxylase 1) (Gibberellin 2-oxidase 1) (GA 2-oxidase 1) (OsGA2ox1) PROTEIN FAMILY: Iron/ascorbate-dependent oxidoreductase family, GA2OX subfamily ORGANISM: Oryza sativa subsp. japonica (Rice) SIMILARITY: Belongs to the iron/ascorbate-dependent oxidoreductase family. GA2OX subfamily. FUNCTION: Catalyzes the 2-beta-hydroxylation of several biologically active gibberellins, leading to the homeostatic regulation of their endogenous level. Catabolism of gibberellins (GAs) plays a central role in plant development. Controls the level of bioactive GAs in the shoot apical meristem, which regulates the vegetative to reproductive phase transition. In vitro, converts GA1, GA4, GA9, GA20, and GA44 to the corresponding 2-beta-hydroxylated products GA8, GA34, GA51, GA29, and GA98, respectively. KEYWORDS: Dioxygenase;Iron;Metal-binding;Oxidoreductase;Reference proteome MISCELLANEOUS: Plants overexpressing GA2OX1 exhibit extremely dwarf phenotype and are unable to achieve phase transition from vegetative to reproductive growth." Q5WA76,"PROTEIN NAMES: U-box domain-containing protein 70 (OsPUB70) (Plant U-box protein 70) (Receptor-like cytoplasmic kinase 197) (OsRLCK197) [Includes: E3 ubiquitin ligase (RING-type E3 ubiquitin transferase); Serine/threonine-protein kinase ] PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family ORGANISM: Oryza sativa subsp. japonica (Rice) SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. FUNCTION: Functions as an E3 ubiquitin ligase. Is recruited by MODD to promote ubiquitination of BZIP46, a positive regulator of abscisic acid (ABA) signaling and drought stress tolerance. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: ATP-binding;Coiled coil;Kinase;Nucleotide-binding;Reference proteome;Repeat;Serine/threonine-protein kinase;TPR repeat;Transferase;Ubl conjugation pathway " Q5WRN1,"PROTEIN NAMES: Tetraspanin-21 PROTEIN FAMILY: Tetraspanin (TM4SF) family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the tetraspanin (TM4SF) family. FUNCTION: Regulates cell fate specification in the postembryonic mesodermal M lineage and body size, probably by positively modulating BMP-like Sma/Mab signaling at the ligand-receptor level. Promotes ventral fate specification in the M lineage, probably by positively modulating lin-12/Notch signaling. KEYWORDS: Cell membrane;Cytoplasmic vesicle;Glycoprotein;Membrane;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Basolateral cell membrane ; Multi-pass membrane protein Cytoplasmic vesicle membrane ; Multi-pass membrane protein Note=Specifically localizes to the basolateral side of intestinal cells. Localizes to cytoplasmic vesicles in the M mesoblast. " Q5XET5,"PROTEIN NAMES: Protein HESO1 (HEN1 suppressor 1) (RNA uridylyltransferase) PROTEIN FAMILY: DNA polymerase type-B-like family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the DNA polymerase type-B-like family. FUNCTION: Uridylates small RNAs to trigger their degradation. Catalyzes the uridylation of 5' fragments produced by AGO1-mediated cleavage of miRNA target RNAs. Acts synergistically with URT1 in unmethylated miRNA uridylation, leading to their degradation. URT1 and HESO1 prefer substrates with different 3' end nucleotides and act cooperatively to tail different forms of the same miRNAs. URT1 and HESO1 act sequentially, with URT1 mono-uridylating the miRNAs followed by their further uridylation by HESO1. URT1 and HESO1 are involved in the uridylation and clearance of RISC-generated 5' mRNA fragments. Able to act on AGO1-bound miRNAs and the uridylated species stay associated with AGO1. KEYWORDS: Cytoplasm;Nucleus;Reference proteome;Transferase SUBCELLULAR LOCATION: Cytoplasm Cytoplasm, P-body Nucleus " Q5XF07,"PROTEIN NAMES: DNA-(apurinic or apyrimidinic site) endonuclease (APEX1-like protein) (Apurinic-apyrimidinic endonuclease) PROTEIN FAMILY: DNA repair enzymes AP/exoA family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the DNA repair enzymes AP/exoA family. FUNCTION: Apurinic/apyrimidinic (AP) endonuclease involved in active DNA demethylation and gene imprinting. According to a report, also displays an in vitro 3'-phosphatase activity. According to another report, has no in vitro 3'-phosphatase activity. Catalyzes the conversion of the 3'-blocking groups 3'-phosphor-alpha,beta-unsaturated aldehyde (3'-PUA) generated by ROS1 to 3'-OH. Has a strong non-specific affinity to DNA. Redundant with APE2 and at least one functional allele is required for seed viability. KEYWORDS: DNA damage;DNA repair;Hydrolase;Magnesium;Metal-binding;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus Nucleus, nucleolus Note=Co-localizes in nucleoplasmic foci with ROS1 and ZDP, two components of the DNA demethylase machinery. " Q5XG87,"PROTEIN NAMES: Terminal nucleotidyltransferase 4A (DNA polymerase sigma) (LAK-1) (Non-canonical poly(A) RNA polymerase PAPD7) (PAP-associated domain-containing protein 7) (TRAMP-like complex polyadenylate polymerase) (Terminal guanylyltransferase) (Terminal uridylyltransferase 5) (TUTase 5) (Topoisomerase-related function protein 4-1) (TRF4-1) PROTEIN FAMILY: DNA polymerase type-B-like family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the DNA polymerase type-B-like family. FUNCTION: Terminal nucleotidyltransferase that catalyzes preferentially the transfer of ATP and GTP on RNA 3' poly(A) tail creating a heterogeneous 3' poly(A) tail leading to mRNAs stabilization by protecting mRNAs from active deadenylation. Also functions as a catalytic subunit of a TRAMP-like complex which has a poly(A) RNA polymerase activity and is involved in a post-transcriptional quality control mechanism. Polyadenylation with short oligo(A) tails is required for the degradative activity of the exosome on several of its nuclear RNA substrates. Has no terminal uridylyltransferase activity, and does not play a role in replication-dependent histone mRNA degradation via uridylation. KEYWORDS: Alternative splicing;ATP-binding;Cytoplasm;Magnesium;Manganese;Metal-binding;mRNA processing;Nucleotide-binding;Nucleotidyltransferase;Nucleus;Reference proteome;Transferase SUBCELLULAR LOCATION: Cytoplasm Nucleus, nucleoplasm Note=Excluded from nucleolus, weak staining detected in the cytoplasm. MISCELLANEOUS: [Isoform 2]: Exhibits poor nucleotidyl transferase activity." Q5XIM7,"PROTEIN NAMES: Lysine--tRNA ligase (Lysyl-tRNA synthetase) (LysRS) PROTEIN FAMILY: Class-II aminoacyl-tRNA synthetase family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family. FUNCTION: Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages (By similarity). Catalyzes the synthesis of the signaling molecule diadenosine tetraphosphate (Ap4A), and thereby mediates disruption of the complex between HINT1 and MITF and the concomitant activation of MITF transcriptional activity. KEYWORDS: Acetylation;Aminoacyl-tRNA synthetase;ATP-binding;Cell membrane;Cytoplasm;Ligase;Membrane;Nucleotide-binding;Nucleus;Phosphoprotein;Protein biosynthesis;Reference proteome;Secreted;Transferase SUBCELLULAR LOCATION: Cytoplasm, cytosol Cytoplasm Nucleus Cell membrane ; Peripheral membrane protein Secreted Note=Secretion is induced by TNF-alpha (By similarity). Cytosolic in quiescent mast cells. Translocates into the nucleus in response to mast cell activation by immunoglobulin E. MISCELLANEOUS: It is likely that the same gene provides both this cytoplasmic isoform and an additional mitochondrial isoform." Q5XM24,PROTEIN NAMES: Autocrine proliferation repressor protein A (PhoPQ-activated pathogenicity-related protein) PROTEIN FAMILY: PqaA family ORGANISM: Dictyostelium discoideum (Social amoeba) SIMILARITY: Belongs to the pqaA family. FUNCTION: Inhibitor that slows proliferation of secreting cells (also known as chalone). May function by binding to cell surface receptors. Requires cfaD for activity. Overexpression slows proliferation. KEYWORDS: Cell cycle;Direct protein sequencing;Glycoprotein;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted Q5XVF0,"PROTEIN NAMES: TATA box-binding protein-associated factor RNA polymerase I subunit B (Central cell guidance protein) (Protein MATERNAL EFFECT EMBRYO ARREST 12) (TATA box-binding protein-associated factor 1B) (TBP-associated factor 1B) PROTEIN FAMILY: RRN7/TAF1B family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the RRN7/TAF1B family. FUNCTION: Component of RNA polymerase I core factor complex that acts as a GTF2B/TFIIB-like factor and plays a key role in multiple steps during transcription initiation such as pre-initiation complex (PIC) assembly and postpolymerase recruitment events in polymerase I (Pol I) transcription. Binds rDNA promoters and plays a role in Pol I recruitment (By similarity). Required for the development of the one-cell zygote and endosperm in embryos. Required for micropylar pollen tube guidance, but has no effect on ovule development and gametophytic cell fate specification. May regulate the transcription of secreted cysteine-rich peptide (CRP) genes in the embryo sac. KEYWORDS: Alternative splicing;DNA-binding;Metal-binding;Nucleus;Reference proteome;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus, nucleolus Nucleus MISCELLANEOUS: Expression of MEE12 in the central cell alone is sufficient to restore the normal pollen tube guidance phenotype." Q5Y4Y6,"PROTEIN NAMES: Gasdermin-A3 (Gasdermin-3) [Cleaved into: Gasdermin-A3, N-terminal (GSDMA3-NT); Gasdermin-A3, C-terminal (GSDMA3-CT)] PROTEIN FAMILY: Gasdermin family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the gasdermin family. FUNCTION: [Gasdermin-A3]: Precursor of a pore-forming protein involved in the transition from catagen to telogen at the end of hair follicle morphogenesis. This form constitutes the precursor of the pore: upon cleavage, the released N-terminal moiety (Gasdermin-A3, N-terminal) binds to membranes and forms pores, triggering pyroptosis. This form acts as a sensor of infection: activation is triggered by cleavage by some bacterial effector protein, which releases the N-terminal moiety (Gasdermin-A3, N-terminal) (By similarity).; FUNCTION: [Gasdermin-A3, N-terminal]: Pore-forming protein that causes membrane permeabilization and pyroptosis. Released upon cleavage by some bacterial effector protein, and binds to membrane inner leaflet lipids (By similarity). Homooligomerizes within the membrane and forms pores of 10-15 nanometers (nm) of inner diameter, allowing the release of mature interleukin-1 (IL1B and IL18) and triggering pyroptosis. Binds to membrane inner leaflet lipids, including bisphosphorylated phosphatidylinositols, such as phosphatidylinositol (4,5)-bisphosphate, as well as phosphatidylinositol (3,4,5)-bisphosphate, and more weakly to monophosphorylated phosphatidylinositols. Also binds to bacterial and mitochondrial lipids, including cardiolipin, and exhibits bactericidal activity. Plays a role in the transition from catagen to telogen at the end of hair follicle morphogenesis, possibly by regulating hair follicle stem cell niche maintenance. Also required for mammary gland development. KEYWORDS: 3D-structure;Cell membrane;Coiled coil;Cytoplasm;Developmental protein;Membrane;Mitochondrion;Necrosis;Reference proteome;Transmembrane;Transmembrane beta strand SUBCELLULAR LOCATION: [Gasdermin-A3]: Cytoplasm, cytosol.; SUBCELLULAR LOCATION: [Gasdermin-A3, N-terminal]: Cell membrane ; Multi-pass membrane protein Mitochondrion membrane ; Multi-pass membrane protein " Q5Y5T1,"PROTEIN NAMES: Palmitoyltransferase ZDHHC20 (Acyltransferase ZDHHC20) (DHHC domain-containing cysteine-rich protein 20) (DHHC-20) (Zinc finger DHHC domain-containing protein 20) PROTEIN FAMILY: DHHC palmitoyltransferase family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the DHHC palmitoyltransferase family. FUNCTION: Palmitoyltransferase that could catalyze the addition of palmitate onto various protein substrates. Catalyzes palmitoylation of Cys residues in the cytoplasmic C-terminus of EGFR, and modulates the duration of EGFR signaling by modulating palmitoylation-dependent EGFR internalization and degradation. Has a preference for acyl-CoA with C16 fatty acid chains. Can also utilize acyl-CoA with C14 and C18 fatty acid chains (By similarity). May palmitoylate CALHM1 subunit of gustatory voltage-gated ion channels and modulate channel gating and kinetics. KEYWORDS: Acyltransferase;Alternative splicing;Cell membrane;Cytoplasm;Endoplasmic reticulum;Golgi apparatus;Lipoprotein;Membrane;Metal-binding;Palmitate;Phosphoprotein;Reference proteome;Transferase;Transmembrane;Transmembrane helix;Zinc SUBCELLULAR LOCATION: Golgi apparatus membrane ; Multi-pass membrane protein Cell membrane ; Multi-pass membrane protein Cytoplasm, perinuclear region Endoplasmic reticulum membrane ; Multi-pass membrane protein Endoplasmic reticulum-Golgi intermediate compartment membrane ; Multi-pass membrane protein " Q5YD48,"PROTEIN NAMES: APOBEC1 complementation factor (APOBEC1-stimulating protein) ORGANISM: Mus musculus (Mouse) FUNCTION: Essential component of the apolipoprotein B mRNA editing enzyme complex which is responsible for the postranscriptional editing of a CAA codon for Gln to a UAA codon for stop in APOB mRNA. Binds to APOB mRNA and is probably responsible for docking the catalytic subunit, APOBEC1, to the mRNA to allow it to deaminate its target cytosine. The complex also seems to protect the edited APOB mRNA from nonsense-mediated decay (By similarity). KEYWORDS: Alternative splicing;Cytoplasm;Endoplasmic reticulum;mRNA processing;Nucleus;Reference proteome;Repeat;RNA-binding SUBCELLULAR LOCATION: Nucleus Endoplasmic reticulum Cytoplasm Note=Predominantly nuclear where it localizes to heterochromatin. Also cytoplasmic where it is found at the outer surface of the endoplasmic reticulum. Shuttles between the nucleus and cytoplasm. May be transported into the nucleus by the nuclear import protein TNPO2/TRN2 or by APOBEC1 (By similarity). MISCELLANEOUS: [Isoform 3]: Minor isoform detected in less than 10% of cDNA clones." Q5YGP8,"PROTEIN NAMES: AP2-like ethylene-responsive transcription factor PLT1 (Protein AINTEGUMENTA-LIKE 3) (Protein PLETHORA 1) PROTEIN FAMILY: AP2/ERF transcription factor family, AP2 subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the AP2/ERF transcription factor family. AP2 subfamily. FUNCTION: Probably acts as a transcriptional activator. Binds to the GCC-box pathogenesis-related promoter element. May be involved in the regulation of gene expression by stress factors and by components of stress signal transduction pathways (By similarity). Master regulator of basal/root fate. Essential for root quiescent center (QC) and columella specification, stem cell activity, as well as for establishment of the stem cell niche during embryogenesis. Modulates the root polar auxin transport by regulating the distribution of PIN genes. Essential role in respecifying pattern and polarity in damaged roots. Direct target of the transcriptional corepressor TPL. Expression levels and patterns regulated post-transcriptionally by root meristem growth factors (RGFs). KEYWORDS: Activator;Auxin signaling pathway;Developmental protein;DNA-binding;Ethylene signaling pathway;Nucleus;Reference proteome;Repeat;Stress response;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " Q5Z807,"PROTEIN NAMES: Zinc finger CCCH domain-containing protein 46 (OsC3H46) (Protein LEAF AND TILLER ANGLE INCREASED CONTROLLER) (OsLIC) ORGANISM: Oryza sativa subsp. japonica (Rice) FUNCTION: Transcriptional activator that binds double-stranded DNA and the single-stranded RNA polymers poly(rA), poly(rU) and poly(rG), but not poly(rC). Mediates optimal plant architecture through brassinosteroid (BR) signaling. May act as a negative regulator in sterol homeostasis. Acts as a negative regulator of BR signaling. Binds to the specific DNA sequence 5'-CTCGC-3' of BZR1 promoter and negatively regulates BZR1. Acts as an antagonistic transcription factor of BZR1 to attenuate the BR signaling pathway and regulate leaf bending. Represses the expression of ILI1, and activates that of IBH1 to balance the regulation activity of BZR1. KEYWORDS: Activator;Brassinosteroid signaling pathway;Cytoplasm;DNA-binding;Metal-binding;Nucleus;Reference proteome;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Cytoplasm Note=Brassinosteroid promotes nuclear localization. Phosphorylation represses nuclear localization. MISCELLANEOUS: Plants silencing LIC are short, have increased leaf and tiller angles, and display both reduced number of rachises and seeds. Plants over-expressing LIC show erect leaves." Q5ZIZ4,"PROTEIN NAMES: Cytosolic purine 5'-nucleotidase (Cytosolic nucleoside phosphotransferase 5'N) PROTEIN FAMILY: 5'(3')-deoxyribonucleotidase family ORGANISM: Gallus gallus (Chicken) SIMILARITY: Belongs to the 5'(3')-deoxyribonucleotidase family. FUNCTION: Broad specificity cytosolic 5'-nucleotidase that catalyzes the dephosphorylation of 6-hydroxypurine nucleoside 5'-monophosphates. In addition, possesses a phosphotransferase activity by which it can transfer a phosphate from a donor nucleoside monophosphate to an acceptor nucleoside, preferably inosine, deoxyinosine and guanosine (By similarity). Has the highest activities for IMP and GMP followed by dIMP, dGMP and XMP. Could also catalyze the transfer of phosphates from pyrimidine monophosphates but with lower efficiency (By similarity). Through these activities regulates the purine nucleoside/nucleotide pools within the cell. KEYWORDS: Allosteric enzyme;ATP-binding;Cytoplasm;Hydrolase;Magnesium;Metal-binding;Nucleotide metabolism;Nucleotide-binding;Reference proteome;Transferase SUBCELLULAR LOCATION: Cytoplasm, cytosol " Q5ZL13,"PROTEIN NAMES: Acyl-coenzyme A diphosphatase NUDT19 (Nucleoside diphosphate-linked moiety X motif 19) (Nudix motif 19) PROTEIN FAMILY: Nudix hydrolase family ORGANISM: Gallus gallus (Chicken) SIMILARITY: Belongs to the Nudix hydrolase family. FUNCTION: Fatty acyl-coenzyme A (CoA) diphosphatase that hydrolyzes fatty acyl-CoA to yield acyl-4'-phosphopantetheine and adenosine 3',5'-bisphosphate (By similarity). Mediates the hydrolysis of a wide range of CoA esters, including choloyl-CoA and branched-chain fatty-acyl-CoA esters and at low substrate concentrations medium and long-chain fatty-acyl-CoA esters are the primary substrates (By similarity). Highest activity seen with medium-chain acyl-CoA esters and higher rates of activity seen with the unsaturated acyl-CoA esters compared with the saturated esters (By similarity). Exhibits decapping activity towards dpCoA-capped RNAs in vitro (By similarity). KEYWORDS: Hydrolase;Magnesium;Manganese;Metal-binding;Peroxisome;Reference proteome SUBCELLULAR LOCATION: Peroxisome " Q5ZM91,PROTEIN NAMES: cAMP-dependent protein kinase type I-alpha regulatory subunit PROTEIN FAMILY: CAMP-dependent kinase regulatory chain family ORGANISM: Gallus gallus (Chicken) SIMILARITY: Belongs to the cAMP-dependent kinase regulatory chain family. KEYWORDS: Acetylation;cAMP;cAMP-binding;Cell membrane;Disulfide bond;Membrane;Nucleotide-binding;Phosphoprotein;Reference proteome;Repeat SUBCELLULAR LOCATION: Cell membrane Q5ZPR3,"PROTEIN NAMES: CD276 antigen (4Ig-B7-H3) (B7 homolog 3) (B7-H3) (Costimulatory molecule) (CD antigen CD276) PROTEIN FAMILY: Immunoglobulin superfamily, BTN/MOG family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the immunoglobulin superfamily. BTN/MOG family. FUNCTION: May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling. KEYWORDS: Alternative splicing;Direct protein sequencing;Disulfide bond;Glycoprotein;Immunoglobulin domain;Membrane;Phosphoprotein;Reference proteome;Repeat;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Single-pass type I membrane protein MISCELLANEOUS: B7-H3 locus underwent genomic duplication leading to tandemly repeated immunoglobulin-like V and C domains (VC domains). The dominantly expressed human B7-H3 isoform contains tandemly duplicated VC domains. In contrast, mouse B7-H3 transcript contains only one single VC domain form due to an exon structure corresponding to V domain-(pseudoexon C)-(pseudoexon V)-C domain. This duplication appearing in primates is suggested to be very recent supporting a model of multiple independent emergence of tandem VC repeats within human and monkey species.; MISCELLANEOUS: [Isoform 1]: Contains tandemly repeated immunoglobulin-like V and C domains.; MISCELLANEOUS: [Isoform 2]: Minor transcript. Contains one single set of immunoglobulin-like V and C domains.; MISCELLANEOUS: [Isoform 3]: Contains tandemly repeated immunoglobulin-like V and C domains.; MISCELLANEOUS: [Isoform 4]: Contains tandemly repeated immunoglobulin-like V and C domains." Q5ZTK6,"PROTEIN NAMES: Calmodulin-dependent glutamylase SidJ ORGANISM: Legionella pneumophila subsp. pneumophila (strain Philadelphia 1 / ATCC 33152 / DSM 7513) FUNCTION: Glutamylase that mediates the covalent attachment of glutamate moieties to SdeA on one of the catalytic residues that is required for its mono-ADP-ribosyltransferase activity. In turn, inhibits SdeA ubiquitinating activity. Glutamylates also related SdeB, SdeC and SidE. Glutamylase activity only occurs in the host since it requires host calmodulin. May also reverse the SdeA-mediated substrate ubiquitination by cleaving the phosphodiester bond that links phosphoribosylated ubiquitin to protein substrates via its deubiquitinase activity. KEYWORDS: 3D-structure;Hydrolase;Ligase;Magnesium;Metal-binding;Multifunctional enzyme;NAD;Nucleotide-binding;Protease;Reference proteome;Thiol protease;Transferase;Virulence " Q60358,"PROTEIN NAMES: L-tyrosine/L-aspartate decarboxylase (TDC/ADC) PROTEIN FAMILY: Group II decarboxylase family, MfnA subfamily ORGANISM: Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) SIMILARITY: Belongs to the group II decarboxylase family. MfnA subfamily. FUNCTION: Catalyzes the decarboxylation of L-tyrosine to produce tyramine for methanofuran biosynthesis. Can also catalyze the decarboxylation of L-aspartate to produce beta-alanine for coenzyme A (CoA) biosynthesis. PATHWAY: Cofactor biosynthesis; methanofuran biosynthesis.; PATHWAY: Cofactor biosynthesis; coenzyme A biosynthesis. KEYWORDS: 3D-structure;Decarboxylase;Lyase;Pyridoxal phosphate;Reference proteome " Q60528,"PROTEIN NAMES: Mucin-1 (MUC-1) (CD antigen CD227) [Cleaved into: Mucin-1 subunit alpha (MUC1-NT) (MUC1-alpha); Mucin-1 subunit beta (MUC1-beta) (MUC1-CT)] ORGANISM: Mesocricetus auratus (Golden hamster) FUNCTION: The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack (By similarity).; FUNCTION: The beta subunit contains a C-terminal domain which is involved in cell signaling, through phosphorylations and protein-protein interactions. Modulates signaling in ERK, Src and NF-kappaB pathways. In activated T-cells, influences directly or indirectly the Ras/MAPK pathway. Promotes tumor progression. Regulates P53-mediated transcription and determines cell fate in the genotoxic stress response. Binds, together with KLF4, the PE21 promoter element of P53 and represses P53 activity (By similarity). KEYWORDS: Autocatalytic cleavage;Cell membrane;Cytoplasm;Glycoprotein;Lipoprotein;Membrane;Nucleus;Palmitate;Phosphoprotein;Reference proteome;Repeat;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Apical cell membrane; Single-pass type I membrane protein. Note=Exclusively located in the apical domain of the plasma membrane of highly polarized epithelial cells. After endocytosis, internalized and recycled to the cell membrane. Located to microvilli and to the tips of long filopodial protusions (By similarity).; SUBCELLULAR LOCATION: [Mucin-1 subunit beta]: Cell membrane. Cytoplasm. Nucleus. Note=On EGF and PDGFRB stimulation, transported to the nucleus through interaction with CTNNB1, a process which is stimulated by phosphorylation. On HRG stimulation, colocalizes with JUP/gamma-catenin at the nucleus. " Q60641,"PROTEIN NAMES: Bile acid receptor (Farnesoid X-activated receptor) (Farnesol receptor HRR-1) (Nuclear receptor subfamily 1 group H member 4) (Retinoid X receptor-interacting protein 14) (RXR-interacting protein 14) PROTEIN FAMILY: Nuclear hormone receptor family, NR1 subfamily ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the nuclear hormone receptor family. NR1 subfamily. FUNCTION: Ligand-activated transcription factor. Receptor for bile acids (BAs) such as chenodeoxycholic acid (CDCA), lithocholic acid, deoxycholic acid (DCA) and allocholic acid (ACA). Plays a essential role in BA homeostasis through the regulation of genes involved in BA synthesis, conjugation and enterohepatic circulation. Also regulates lipid and glucose homeostasis and is involved in innate immune response. The FXR-RXR heterodimer binds predominantly to farnesoid X receptor response elements (FXREs) containing two inverted repeats of the consensus sequence 5'-AGGTCA-3' in which the monomers are spaced by 1 nucleotide (IR-1) but also to tandem repeat DR1 sites with lower affinity, and can be activated by either FXR or RXR-specific ligands. It is proposed that monomeric nuclear receptors such as NR5A2/LRH-1 bound to coregulatory nuclear responsive element (NRE) halfsites located in close proximity to FXREs modulate transcriptional activity. In the liver activates transcription of the corepressor NR0B2 thereby indirectly inhibiting CYP7A1 and CYP8B1 (involved in BA synthesis) implicating at least in part histone demethylase KDM1A resulting in epigenomic repression, and SLC10A1/NTCP (involved in hepatic uptake of conjugated BAs). Activates transcription of the repressor MAFG (involved in regulation of BA synthesis). Activates transcription of SLC27A5/BACS and BAAT (involved in BA conjugation), ABCB11/BSEP (involved in bile salt export) by directly recruiting histone methyltransferase CARM1, and ABCC2/MRP2 (involved in secretion of conjugated BAs) and ABCB4 (involved in secretion of phosphatidylcholine in the small intestine). In ileal enterocytes activates FABP6/IBABP (involved in cytosolic transport), SLC51A/OSTA and SLC51B/OSTB (involved in secretion of conjugated BAs to the portal blood), and repressor NR0B2/SHP thereby indirectly inhibiting SLC10A2/ASBT (involved in BA uptake) (By similarity). In the intestine activates FGF15 expression and secretion leading to hepatic CYP7A1 repression; the function also involves the coordinated induction of hepatic KLB/beta-klotho expression. Transcriptional activation of FABP6/IBAP and SCD1 but not of ABCB11 is isoform-specific. Regulates transcription of liver UGT2B4 and SULT2A1 involved in BA detoxification; binding to the UGT2B4 promoter seems to imply a monomeric transactivation independent of RXRA (By similarity). Modulates lipid homeostasis by activating liver NR0B2/SHP-mediated repression of SREBF1 isoform SREBP-1C (involved in de novo lipogenesis), expression of PLTP (involved in HDL formation), SCARB1 (involved in HDL hepatic uptake), APOE, APOC1, APOC4, VLDLR and SDC1 (involved in the hepatic uptake of LDL and IDL remnants), and inhibiting expression of MTTP (involved in VLDL assembly). Increases expression of APOC2 (promoting lipoprotein lipase activity implicated in triglyceride clearance). Transrepresses APOA1 probably involving a monomeric competition with NR2A1 for binding to a DR1 element. Also reduces triglyceride clearance by inhibiting expression of ANGPTL3 and APOC3 (both involved in inhibition of lipoprotein lipase). Involved in glucose homeostasis by modulating hepatic gluconeogenesis through activation of NR0B2/SHP-mediated repression of respective genes. Modulates glycogen synthesis (inducing phosphorylation of glycogen synthase kinase-3). Modulates glucose-stimulated insulin secretion and is involved in insulin resistance. Involved in intestinal innate immunity. Plays a role in protecting the distal small intestine against bacterial overgrowth and preservation of the epithelial barrier. Down-regulates inflammatory cytokine expression in several types of immune cells including macrophages and mononuclear cells. Mediates transrepression of TLR4-induced cytokine expression; the function seems to require its sumoylation and prevents N-CoR nuclear receptor corepressor clearance from target genes such as IL1B and NOS2 (By similarity). Involved in the TLR9-mediated protective mechanism in intestinal inflammation. Plays a anti-inflammatory role in liver inflammation; proposed to inhibit pro-inflammatory (but not antiapoptotic) NF-kappa-B signaling.; FUNCTION: [Isoform 2]: Activates transcription of IBAP and SDC1.; FUNCTION: [Isoform 4]: Activates transcription of IBAP and SDC1. KEYWORDS: Acetylation;Activator;Alternative promoter usage;Alternative splicing;DNA-binding;Immunity;Inflammatory response;Innate immunity;Isopeptide bond;Metal-binding;Methylation;Nucleus;Phosphoprotein;Receptor;Reference proteome;Repressor;Transcription;Transcription regulation;Tumor suppressor;Ubl conjugation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: Mouse Nr1h4/FXR is less responsive to CDCA and more responsive to cholic acid (CA) than human FXR.; MISCELLANEOUS: [Isoform 1]: Produced by alternative promoter usage.; MISCELLANEOUS: [Isoform 2]: Produced by alternative splicing of isoform 1.; MISCELLANEOUS: [Isoform 3]: Produced by alternative promoter usage.; MISCELLANEOUS: [Isoform 4]: Produced by alternative splicing of isoform 3." Q60654,PROTEIN NAMES: Killer cell lectin-like receptor 7 (Lymphocyte antigen 49g) (Ly-49g) (T-cell surface glycoprotein Ly-49G) ORGANISM: Mus musculus (Mouse) FUNCTION: Receptor on natural killer (NK) cells for class I MHC. KEYWORDS: 3D-structure;Alternative splicing;Cell adhesion;Disulfide bond;Glycoprotein;Lectin;Membrane;Receptor;Reference proteome;Signal-anchor;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane; Single-pass type II membrane protein. Q60837,"PROTEIN NAMES: Interleukin-12 receptor subunit beta-1 (IL-12 receptor subunit beta-1) (IL-12R subunit beta-1) (IL-12R-beta-1) (IL-12 receptor beta component) (CD antigen CD212) PROTEIN FAMILY: Type I cytokine receptor family, Type 2 subfamily ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the type I cytokine receptor family. Type 2 subfamily. FUNCTION: Functions as an interleukin receptor which binds interleukin-12 with low affinity and is involved in IL12 transduction. Associated with IL12RB2 it forms a functional, high affinity receptor for IL12. Associates also with IL23R to form the interleukin-23 receptor which functions in IL23 signal transduction probably through activation of the Jak-Stat signaling cascade. KEYWORDS: 3D-structure;Disulfide bond;Glycoprotein;Membrane;Receptor;Reference proteome;Repeat;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. " Q60953,"PROTEIN NAMES: Protein PML ORGANISM: Mus musculus (Mouse) FUNCTION: Functions via its association with PML-nuclear bodies (PML-NBs) in a wide range of important cellular processes, including tumor suppression, transcriptional regulation, apoptosis, senescence, DNA damage response, and viral defense mechanisms. Acts as the scaffold of PML-NBs allowing other proteins to shuttle in and out, a process which is regulated by SUMO-mediated modifications and interactions. Inhibits EIF4E-mediated mRNA nuclear export by reducing EIF4E affinity for the 5' 7-methylguanosine (m7G) cap of target mRNAs (By similarity). Positively regulates p53/TP53 by acting at different levels (by promoting its acetylation and phosphorylation and by inhibiting its MDM2-dependent degradation). Regulates phosphorylation of ITPR3 and plays a role in the regulation of calcium homeostasis at the endoplasmic reticulum. Regulates RB1 phosphorylation and activity. Acts as both a negative regulator of PPARGC1A acetylation and a potent activator of PPAR signaling and fatty acid oxidation. Regulates translation of HIF1A by sequestering MTOR, and thereby plays a role in neoangiogenesis and tumor vascularization. Regulates PER2 nuclear localization and circadian function. Cytoplasmic PML is involved in the regulation of the TGF-beta signaling pathway. Required for normal development of the brain cortex during embryogenesis. Plays a role in granulopoiesis or monopoiesis of myeloid progenitor cells. May play a role regulating stem and progenitor cell fate in tissues as diverse as blood, brain and breast. Shows antiviral activity towards lymphocytic choriomeningitis virus (LCMV) and the vesicular stomatitis virus (VSV). KEYWORDS: Acetylation;Activator;Alternative splicing;Antiviral defense;Apoptosis;Biological rhythms;Coiled coil;Cytoplasm;DNA-binding;Endoplasmic reticulum;Endosome;Host-virus interaction;Immunity;Innate immunity;Isopeptide bond;Membrane;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Repeat;Transcription;Transcription regulation;Tumor suppressor;Ubl conjugation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Nucleus, nucleoplasm. Cytoplasm. Nucleus, PML body. Nucleus, nucleolus Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Early endosome membrane ; Peripheral membrane protein ; Cytoplasmic side Note=Detected in the nucleolus after DNA damage. Acetylation at Lys-497 is essential for its nuclear localization. Within the nucleus, most of PML is expressed in the diffuse nuclear fraction of the nucleoplasm and only a small fraction is found in the matrix-associated nuclear bodies (PML-NBs). The transfer of PML from the nucleoplasm to PML-NBs depends on its phosphorylation and sumoylation. The B1 box and the RING finger are also required for the localization in PML-NBs. Also found in specific membrane structures termed mitochondria-associated membranes (MAMs) which connect the endoplasmic reticulum (ER) and the mitochondria (By similarity). " Q60HE8,"PROTEIN NAMES: Mucolipin-1 (Mucolipidin) (Transient receptor potential channel mucolipin 1) (TRPML1) PROTEIN FAMILY: Transient receptor family, Polycystin subfamily, MCOLN1 sub-subfamily ORGANISM: Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) SIMILARITY: Belongs to the transient receptor (TC 1.A.4) family. Polycystin subfamily. MCOLN1 sub-subfamily. FUNCTION: Nonselective cation channel probably playing a role in the regulation of membrane trafficking events and of metal homeostasis. Proposed to play a major role in Ca(2+) release from late endosome and lysosome vesicles to the cytoplasm, which is important for many lysosome-dependent cellular events, including the fusion and trafficking of these organelles, exocytosis and autophagy. Required for efficient uptake of large particles in macrophages in which Ca(2+) release from the lysosomes triggers lysosomal exocytosis. May also play a role in phagosome-lysosome fusion. Involved in lactosylceramide trafficking indicative for a role in the regulation of late endocytic membrane fusion/fission events. By mediating lysosomal Ca(2+) release is involved in regulation of mTORC1 signaling and in mTOR/TFEB-dependent lysosomal adaptation to environmental cues such as nutrient levels. Seems to act as lysosomal active oxygen species (ROS) sensor involved in ROS-induced TFEB activation and autophagy. Functions as a Fe(2+) permeable channel in late endosomes and lysosomes. Proposed to play a role in zinc homeostasis probably implicating its association with TMEM163. In adaptive immunity, TRPML2 and TRPML1 may play redundant roles in the function of the specialized lysosomes of B cells.; FUNCTION: May contribute to cellular lipase activity within the late endosomal pathway or at the cell surface which may be involved in processes of membrane reshaping and vesiculation, especially the growth of tubular structures. However, it is not known, whether it conveys the enzymatic activity directly, or merely facilitates the activity of an associated phospholipase. KEYWORDS: Adaptive immunity;Calcium;Calcium transport;Cell membrane;Cell projection;Cytoplasmic vesicle;Disulfide bond;Endosome;Glycoprotein;Immunity;Ion channel;Ion transport;Lipid-binding;Lysosome;Membrane;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Late endosome membrane ; Multi-pass membrane protein Lysosome membrane ; Multi-pass membrane protein Cytoplasmic vesicle membrane ; Multi-pass membrane protein Cell projection, phagocytic cup Cytoplasmic vesicle, phagosome membrane ; Multi-pass membrane protein Cell membrane ; Multi-pass membrane protein Note=Delivery from the trans-Golgi to lysosomes seems to occur mainly in a direct intracellular manner without intermediate delivery to the plasma membrane. Under normal conditions, restricted to intracellular compartments so that only a very minor proportion is present at the cell membrane. " Q61066,"PROTEIN NAMES: Nuclear receptor subfamily 0 group B member 1 (Nuclear receptor DAX-1) PROTEIN FAMILY: Nuclear hormone receptor family, NR0 subfamily ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the nuclear hormone receptor family. NR0 subfamily. FUNCTION: Orphan nuclear receptor. Component of a cascade required for the development of the hypothalamic-pituitary-adrenal-gonadal axis. Acts as a coregulatory protein that inhibits the transcriptional activity of other nuclear receptors through heterodimeric interactions. May also have a role in the development of the embryo and in the maintenance of embryonic stem cell pluripotency (By similarity). KEYWORDS: 3D-structure;Cytoplasm;Nucleus;Receptor;Reference proteome;Repeat;Repressor;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus Cytoplasm Note=Shuttles between the cytoplasm and nucleus. Homodimers exits in the cytoplasm and in the nucleus (By similarity). " Q61072,"PROTEIN NAMES: Disintegrin and metalloproteinase domain-containing protein 9 (ADAM 9) (Meltrin-gamma) (Metalloprotease/disintegrin/cysteine-rich protein 9) (Myeloma cell metalloproteinase) ORGANISM: Mus musculus (Mouse) FUNCTION: Metalloprotease that cleaves and releases a number of molecules with important roles in tumorigenesis and angiogenesis, such as TEK, KDR, EPHB4, CD40, VCAM1 and CDH5. May mediate cell-cell, cell-matrix interactions and regulate the motility of cells via interactions with integrins. KEYWORDS: Cell membrane;Direct protein sequencing;Disulfide bond;EGF-like domain;Glycoprotein;Hydrolase;Membrane;Metal-binding;Metalloprotease;Phosphoprotein;Protease;Reference proteome;Signal;Transmembrane;Transmembrane helix;Zinc;Zymogen SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein " Q61210,"PROTEIN NAMES: Rho guanine nucleotide exchange factor 1 (Lbc's second cousin) (Lymphoid blast crisis-like 2) ORGANISM: Mus musculus (Mouse) FUNCTION: Seems to play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13) subunits. Acts as a GTPase-activating protein (GAP) for GNA12 and GNA13, and as guanine nucleotide exchange factor (GEF) for RhoA GTPase. Activated G alpha 13/GNA13 stimulates the RhoGEF activity through interaction with the RGS-like domain. This GEF activity is inhibited by binding to activated GNA12. Mediates angiotensin-2-induced RhoA activation. Isoform 3 and isoform 4 do not homooligomerize and show an enhanced RhoGEF activity. KEYWORDS: Alternative splicing;Coiled coil;Cytoplasm;GTPase activation;Guanine-nucleotide releasing factor;Membrane;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Membrane Note=Translocated to the membrane by activated GNA13 or LPA stimulation. " Q61220,"PROTEIN NAMES: Protein kinase C-binding protein NELL2 (MEL91 protein) (NEL-like protein 2) ORGANISM: Mus musculus (Mouse) FUNCTION: Plays multiple roles in neural tissues, regulates neuronal proliferation, survival, differentiation, polarization, as well as axon guidance and synaptic functions. Plays an important role in axon development during neuronal differentiation through the MAPK intracellular signaling pathway (By similarity). Via binding to its receptor ROBO3, plays a role in axon guidance, functioning as a repulsive axon guidance cue that contributes to commissural axon guidance to the midline. Required for neuron survival through the modulation of MAPK signaling pathways too. Involved in the regulation of hypothalamic GNRH secretion and the control of puberty (By similarity).; FUNCTION: Epididymal-secreted protein that signals through a ROS1-pathway to regulate the epididymal initial segment (IS) maturation, sperm maturation and male fertility. KEYWORDS: Calcium;Disulfide bond;EGF-like domain;Glycoprotein;Metal-binding;Reference proteome;Repeat;Secreted;Signal SUBCELLULAR LOCATION: Secreted Note=Detected in the epididymal lumen. " Q61382,"PROTEIN NAMES: TNF receptor-associated factor 4 (Cysteine-rich motif associated to RING and Traf domains protein 1) PROTEIN FAMILY: TNF receptor-associated factor family, B subfamily ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the TNF receptor-associated factor family. B subfamily. FUNCTION: Adapter protein with E3 ligase activity that is involved in many diverse biological processes including cell proliferation, migration, differentiation, DNA repair, platelet activation or apoptosis. Promotes EGFR-mediated signaling by facilitating the dimerization of EGFR and downstream AKT activation thereby promoting cell proliferation. Ubiquitinates SMURF2 through 'Lys-48'-linked ubiquitin chain leading to SMURF2 degradation through the proteasome and subsequently osteogenic differentiation. Promotes 'Lys-63'-mediated ubiquitination of CHK1 which in turn activates cell cycle arrest and activation of DNA repair. In addition, promotes an atypical 'Lys-29'-linked ubiquitination at the C-terminal end of IRS1 which is crucial for insulin-like growth factor (IGF) signal transduction (By similarity). Regulates activation of NF-kappa-B in response to signaling through Toll-like receptors. Required for normal skeleton development, and for normal development of the respiratory tract. Required for activation of RPS6KB1 in response to TNF signaling. Modulates TRAF6 functions. Inhibits adipogenic differentiation by activating pyruvate kinase PKM activity and subsequently the beta-catenin signaling pathway (By similarity). PATHWAY: Protein degradation; proteasomal ubiquitin-dependent pathway. KEYWORDS: Apoptosis;Cell junction;Cell membrane;Coiled coil;Cytoplasm;Cytoskeleton;Developmental protein;Immunity;Innate immunity;Isopeptide bond;Membrane;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Repeat;Tight junction;Transferase;Ubl conjugation;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cytoplasm Nucleus Cytoplasm, perinuclear region Cell junction, tight junction Cell membrane ; Peripheral membrane protein ; Cytoplasmic side Cytoplasm, cytoskeleton " Q61584,"PROTEIN NAMES: RNA-binding protein FXR1 (FXR1P) (mFxr1p) PROTEIN FAMILY: FMR1 family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the FMR1 family. FUNCTION: mRNA-binding protein that acts as a regulator of mRNAs translation and/or stability, and which is required for various processes, such as neurogenesis, muscle development and spermatogenesis. Specifically binds to AU-rich elements (AREs) in the 3'-UTR of target mRNAs. Promotes formation of some phase-separated membraneless compartment by undergoing liquid-liquid phase separation upon binding to AREs-containing mRNAs, leading to assemble mRNAs into cytoplasmic ribonucleoprotein granules that concentrate mRNAs with associated regulatory factors. Required to activate translation of stored mRNAs during late spermatogenesis: acts by undergoing liquid-liquid phase separation to assemble target mRNAs into cytoplasmic ribonucleoprotein granules that recruit translation initiation factor EIF4G3 to activate translation of stored mRNAs in late spermatids. Promotes translation of MYC transcripts by recruiting the eIF4F complex to the translation start site (By similarity). Acts as a negative regulator of inflammation in response to IL19 by promoting destabilization of pro-inflammatory transcripts (By similarity). Also acts as an inhibitor of inflammation by binding to TNF mRNA, decreasing TNF protein production. Acts as a negative regulator of AMPA receptor GRIA2/GluA2 synthesis during long-lasting synaptic potentiation of hippocampal neurons by binding to GRIA2/GluA2 mRNA, thereby inhibiting its translation. Regulates proliferation of adult neural stem cells by binding to CDKN1A mRNA and promoting its expression. Acts as a regulator of sleep and synaptic homeostasis by regulating translation of transcripts in neurons. Required for embryonic and postnatal development of muscle tissue by undergoing liquid-liquid phase separation to assemble target mRNAs into cytoplasmic ribonucleoprotein granules. Involved in the nuclear pore complex localization to the nuclear envelope by preventing cytoplasmic aggregation of nucleoporins: acts by preventing ectopic phase separation of nucleoporins in the cytoplasm via a microtubule-dependent mechanism (By similarity). KEYWORDS: Acetylation;Alternative splicing;Cell projection;Cytoplasm;Developmental protein;Differentiation;Isopeptide bond;Methylation;Myogenesis;Nucleus;Phosphoprotein;Reference proteome;Repeat;RNA-binding;Spermatogenesis;Synapse;Ubl conjugation SUBCELLULAR LOCATION: Cytoplasm, Cytoplasmic ribonucleoprotein granule Cytoplasm, Stress granule Cytoplasm Cell projection, dendrite Cell projection, dendritic spine Cell projection, axon Nucleus envelope Postsynapse Note=Specifically localizes to cytoplasmic ribonucleoprotein membraneless compartments. Localizes to stress granules following phosphorylation at Ser-449 by PAK1 (By similarity). Adjacent to Z-lines in muscles. " Q62233,"PROTEIN NAMES: Homeobox protein SIX3 (Sine oculis homeobox homolog 3) PROTEIN FAMILY: SIX/Sine oculis homeobox family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the SIX/Sine oculis homeobox family. FUNCTION: Transcriptional regulator which can act as both a transcriptional repressor and activator by binding a ATTA homeodomain core recognition sequence on these target genes. During forebrain development represses WNT1 expression allowing zona limitans intrathalamica formation and thereby ensuring proper anterio-posterior patterning of the diencephalon and formation of the rostral diencephalon. Acts as a direct upstream activator of SHH expression in the rostral diencephalon ventral midline and that in turn SHH maintains its expression. In addition, Six3 activity is required for the formation of the telencephalon. During postnatal stages of brain development is necessary for ependymal cell maturation by promoting the maturation of radial glia into ependymal cells through regulation of neuroblast proliferation and migration. Acts on the proliferation and differentiation of neural progenitor cells through activating transcription of CCND1 AND CCND2. During early lens formation plays a role in lens induction and specification by activating directly PAX6 in the presumptive lens ectoderm. In turn PAX6 activates SIX3 resulting in activation of PDGFRA and CCND1 promoting cell proliferation. Also is required for the neuroretina development by directly suppressing WNT8B expression in the anterior neural plate territory. Its action during retina development and lens morphogenesis is TLE5 and TLE4-dependent manner. Furthermore, during eye development regulates several genes expression. Before and during early lens development represses the CRYGF promoter by binding a SIX repressor element. Directly activates RHO transcription, or cooperates with CRX or NRL. Six3 functions also in the formation of the proximodistal axis of the optic cup , and promotes the formation of optic vesicles-like structures. During pituitary development, acts in parallel or alternatively with HESX1 to control cell proliferation through Wnt/beta-catenin pathway. Plays a role in eye development by suppressing WNT1 expression and in dorsal-ventral patterning by repressing BMP signaling pathway (By similarity). KEYWORDS: Alternative splicing;Developmental protein;DNA-binding;Homeobox;Nucleus;Reference proteome;Repressor;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " Q62312,"PROTEIN NAMES: TGF-beta receptor type-2 (TGFR-2) (TGF-beta type II receptor) (Transforming growth factor-beta receptor type II) (TGF-beta receptor type II) (TbetaR-II) PROTEIN FAMILY: Protein kinase superfamily, TKL Ser/Thr protein kinase family, TGFB receptor subfamily ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily. FUNCTION: Transmembrane serine/threonine kinase forming with the TGF-beta type I serine/threonine kinase receptor, TGFBR1, the non-promiscuous receptor for the TGF-beta cytokines TGFB1, TGFB2 and TGFB3. Transduces the TGFB1, TGFB2 and TGFB3 signal from the cell surface to the cytoplasm and is thus regulating a plethora of physiological and pathological processes including cell cycle arrest in epithelial and hematopoietic cells, control of mesenchymal cell proliferation and differentiation, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. The formation of the receptor complex composed of 2 TGFBR1 and 2 TGFBR2 molecules symmetrically bound to the cytokine dimer results in the phosphorylation and the activation of TGFRB1 by the constitutively active TGFBR2. Activated TGFBR1 phosphorylates SMAD2 which dissociates from the receptor and interacts with SMAD4. The SMAD2-SMAD4 complex is subsequently translocated to the nucleus where it modulates the transcription of the TGF-beta-regulated genes. This constitutes the canonical SMAD-dependent TGF-beta signaling cascade. Also involved in non-canonical, SMAD-independent TGF-beta signaling pathways (By similarity).; FUNCTION: [Isoform RII-1]: Has transforming growth factor beta-activated receptor activity.; FUNCTION: [Isoform RII-2]: Has transforming growth factor beta-activated receptor activity. KEYWORDS: Alternative splicing;Apoptosis;ATP-binding;Cell membrane;Differentiation;Disulfide bond;Glycoprotein;Growth regulation;Kinase;Magnesium;Manganese;Membrane;Metal-binding;Nucleotide-binding;Phosphoprotein;Receptor;Reference proteome;Serine/threonine-protein kinase;Signal;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein Membrane raft " Q62720,"PROTEIN NAMES: Proton-coupled zinc antiporter SLC30A1 (Solute carrier family 30 member 1) (Zinc transporter 1) (ZnT-1) PROTEIN FAMILY: Cation diffusion facilitator (CDF) transporter family, SLC30A subfamily ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. SLC30A subfamily. FUNCTION: Zinc ion:proton antiporter that could function at the plasma membrane mediating zinc efflux from cells against its electrochemical gradient protecting them from intracellular zinc accumulation and toxicity. Alternatively, could prevent the transport to the plasma membrane of CACNB2, the L-type calcium channels regulatory subunit, through a yet to be defined mechanism. By modulating the expression of these channels at the plasma membrane, could prevent calcium and zinc influx into cells. By the same mechanism, could also prevent L-type calcium channels-mediated heavy metal influx into cells. In some cells, could also function as a zinc ion:proton antiporter mediating zinc entry into the lumen of cytoplasmic vesicles. In macrophages, can increase zinc ions concentration into the lumen of cytoplasmic vesicles containing engulfed bacteria and could help inactivate them (By similarity). KEYWORDS: Antiport;Cell membrane;Cytoplasmic vesicle;Glycoprotein;Ion transport;Membrane;Metal-binding;Phosphoprotein;Reference proteome;Repeat;Transmembrane;Transmembrane helix;Transport;Zinc;Zinc transport SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Basolateral cell membrane ; Multi-pass membrane protein Cytoplasmic vesicle membrane ; Multi-pass membrane protein Note=Localization to the plasma membrane is regulated by cellular zinc status. Recruitment to the plasma membrane from an internal pool is stimulated by zinc while in absence of zinc the plasma membrane pool is endocytosed and degraded (By similarity). Localizes to the basolateral surface of enterocytes. Localizes to zinc-containing intracellular vesicles in macrophages (By similarity). " Q62758,"PROTEIN NAMES: 5-hydroxytryptamine receptor 4 (5-HT-4) (5-HT4) (Serotonin receptor 4) PROTEIN FAMILY: G-protein coupled receptor 1 family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the G-protein coupled receptor 1 family. FUNCTION: This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that stimulate adenylate cyclase. KEYWORDS: Alternative splicing;Cell membrane;Disulfide bond;Endosome;G-protein coupled receptor;Glycoprotein;Lipoprotein;Membrane;Palmitate;Receptor;Reference proteome;Transducer;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Endosome. Note=Interaction with SNX27 mediates recruitment to early endosomes, while interaction with NHERF1 and EZR might target the protein to specialized subcellular regions, such as microvilli. " Q62848,"PROTEIN NAMES: ADP-ribosylation factor GTPase-activating protein 1 (ARF GAP 1) (ADP-ribosylation factor 1 GTPase-activating protein) (ARF1 GAP) (ARF1-directed GTPase-activating protein) ORGANISM: Rattus norvegicus (Rat) FUNCTION: GTPase-activating protein (GAP) for the ADP ribosylation factor 1 (ARF1). Involved in membrane trafficking and /or vesicle transport. Promotes hydrolysis of the ARF1-bound GTP and thus, is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles, a prerequisite for vesicle's fusion with target compartment. Probably regulates ARF1-mediated transport via its interaction with the KDELR proteins and TMED2. Overexpression induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, as when ARF1 is deactivated. Its activity is stimulated by phosphoinosides and inhibited by phosphatidylcholine. KEYWORDS: Acetylation;Alternative splicing;Cytoplasm;Direct protein sequencing;ER-Golgi transport;Golgi apparatus;GTPase activation;Metal-binding;Phosphoprotein;Protein transport;Reference proteome;Transport;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cytoplasm. Golgi apparatus. Note=Associates with the Golgi complex. " Q62894,"PROTEIN NAMES: Extracellular matrix protein 1 (Secretory component p85) ORGANISM: Rattus norvegicus (Rat) FUNCTION: Involved in endochondral bone formation as negative regulator of bone mineralization. Stimulates the proliferation of endothelial cells and promotes angiogenesis. Inhibits MMP9 proteolytic activity (By similarity). KEYWORDS: Angiogenesis;Biomineralization;Extracellular matrix;Glycoprotein;Mineral balance;Osteogenesis;Phosphoprotein;Reference proteome;Repeat;Secreted;Signal SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix. " Q62902,"PROTEIN NAMES: Protein ERGIC-53 (ER-Golgi intermediate compartment 53 kDa protein) (Lectin mannose-binding 1) (p58) ORGANISM: Rattus norvegicus (Rat) FUNCTION: Mannose-specific lectin. May recognize sugar residues of glycoproteins, glycolipids, or glycosylphosphatidyl inositol anchors and may be involved in the sorting or recycling of proteins, lipids, or both. The LMAN1-MCFD2 complex forms a specific cargo receptor for the ER-to-Golgi transport of selected proteins (By similarity). KEYWORDS: 3D-structure;Disulfide bond;Endoplasmic reticulum;ER-Golgi transport;Golgi apparatus;Lectin;Membrane;Metal-binding;Phosphoprotein;Protein transport;Reference proteome;Signal;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Endoplasmic reticulum-Golgi intermediate compartment membrane ; Single-pass type I membrane protein Golgi apparatus membrane ; Single-pass membrane protein Endoplasmic reticulum membrane ; Single-pass membrane protein " Q63276,"PROTEIN NAMES: Bile acid-CoA:amino acid N-acyltransferase (BACAT) (BAT) (Bile acid-CoA thioesterase) (Choloyl-CoA hydrolase) (Glycine N-choloyltransferase) (Kan-1) (Long-chain fatty-acyl-CoA hydrolase) PROTEIN FAMILY: C/M/P thioester hydrolase family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the C/M/P thioester hydrolase family. FUNCTION: Catalyzes the amidation of bile acids (BAs) with the amino acids taurine and glycine. More efficient at taurine conjugation of cholyl CoA than glycine conjugation. Amidation of BAs in the liver with glycine or taurine prior to their excretion into bile is an important biochemical event in bile acid metabolism (By similarity). This conjugation (or amidation) plays several important biological roles in that it promotes the secretion of BAs and cholesterol into bile and increases the detergent properties of BAs in the intestine, which facilitates lipid and vitamin absorption (By similarity). May also act as an acyl-CoA thioesterase that regulates intracellular levels of free fatty acids (By similarity). In vitro, catalyzes the hydrolysis of long- and very long-chain saturated acyl-CoAs to the free fatty acid and coenzyme A (CoASH), and conjugates glycine to these acyl-CoAs (By similarity). KEYWORDS: Acyltransferase;Cytoplasm;Fatty acid metabolism;Hydrolase;Lipid metabolism;Peroxisome;Phosphoprotein;Reference proteome;Serine esterase;Transferase SUBCELLULAR LOCATION: Peroxisome Cytoplasm, cytosol " Q63651,"PROTEIN NAMES: Rhodopsin kinase GRK1 (RK) (G protein-coupled receptor kinase 1) PROTEIN FAMILY: Protein kinase superfamily, AGC Ser/Thr protein kinase family, GPRK subfamily ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily. FUNCTION: Retina-specific kinase involved in the signal turnoff via phosphorylation of rhodopsin (RHO), the G protein- coupled receptor that initiates the phototransduction cascade (By similarity). This rapid desensitization is essential for scotopic vision and permits rapid adaptation to changes in illumination (By similarity). May play a role in the maintenance of the outer nuclear layer in the retina (By similarity). KEYWORDS: ATP-binding;Cell projection;Direct protein sequencing;Kinase;Lipoprotein;Membrane;Methylation;Nucleotide-binding;Phosphoprotein;Prenylation;Reference proteome;Sensory transduction;Serine/threonine-protein kinase;Transferase;Vision SUBCELLULAR LOCATION: Membrane ; Lipid-anchor Cell projection, cilium, photoreceptor outer segment Note=Subcellular location is not affected by light or dark conditions. " Q63886,"PROTEIN NAMES: UDP-glucuronosyltransferase 1A1 (UGT1A1) (UDP-glucuronosyltransferase 1-1) (UDPGT 1-1) (UGT1*1) (UGT1-01) (UGT1.1) (UGTBR1) PROTEIN FAMILY: UDP-glycosyltransferase family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the UDP-glycosyltransferase family. FUNCTION: UDP-glucuronosyltransferase (UGT) that catalyzes phase II biotransformation reactions in which lipophilic substrates are conjugated with glucuronic acid to increase the metabolite's water solubility, thereby facilitating excretion into either the urine or bile (By similarity). Essential for the elimination and detoxification of drugs, xenobiotics and endogenous compounds (By similarity). Catalyzes the glucuronidation of endogenous estrogen hormones such as estradiol, estrone and estriol (By similarity). Involved in the glucuronidation of bilirubin, a degradation product occurring in the normal catabolic pathway that breaks down heme in vertebrates (By similarity). Also catalyzes the glucuronidation the isoflavones genistein, daidzein, glycitein, formononetin, biochanin A and prunetin, which are phytoestrogens with anticancer and cardiovascular properties (By similarity). Involved in the glucuronidation of the AGTR1 angiotensin receptor antagonist losartan, a drug which can inhibit the effect of angiotensin II (By similarity). Involved in the biotransformation of 7-ethyl-10-hydroxycamptothecin (SN-38), the pharmacologically active metabolite of the anticancer drug irinotecan (By similarity). KEYWORDS: Alternative splicing;Endoplasmic reticulum;Glycoprotein;Glycosyltransferase;Lipid metabolism;Membrane;Reference proteome;Signal;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Single-pass membrane protein " Q64249,"PROTEIN NAMES: Nuclear receptor subfamily 6 group A member 1 (Germ cell nuclear factor) (GCNF) (mGCNF) (Retinoid receptor-related testis-specific receptor) (RTR) PROTEIN FAMILY: Nuclear hormone receptor family, NR6 subfamily ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the nuclear hormone receptor family. NR6 subfamily. FUNCTION: Orphan nuclear receptor. Binds to a response element containing the sequence 5'-TCAAGGTCA-3'. May be involved in the regulation of gene expression in germ cell development during gametogenesis. KEYWORDS: 3D-structure;Alternative splicing;Developmental protein;Differentiation;DNA-binding;Metal-binding;Nucleus;Receptor;Reference proteome;Spermatogenesis;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus " Q64537,PROTEIN NAMES: Calpain small subunit 1 (CSS1) (Calcium-activated neutral proteinase small subunit) (CANP small subunit) (Calcium-dependent protease small subunit) (CDPS) (Calcium-dependent protease small subunit 1) (Calpain regulatory subunit) ORGANISM: Rattus norvegicus (Rat) FUNCTION: Regulatory subunit of the calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Essential for embryonic development (By similarity). KEYWORDS: 3D-structure;Acetylation;Calcium;Cell membrane;Cytoplasm;Direct protein sequencing;Membrane;Metal-binding;Phosphoprotein;Reference proteome;Repeat SUBCELLULAR LOCATION: Cytoplasm. Cell membrane. Note=Translocates to the plasma membrane upon calcium binding (By similarity). Allows the formation of the homodimer and also appears to mediate the contact between the large catalytic subunit and small regulatory subunit for the formation of the heterodimer. Q64663,PROTEIN NAMES: P2X purinoceptor 7 (P2X7) (ATP receptor) (P2Z receptor) (Purinergic receptor) PROTEIN FAMILY: P2X receptor family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the P2X receptor family. FUNCTION: Receptor for ATP that acts as a ligand-gated ion channel. Responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Could function in both fast synaptic transmission and the ATP-mediated lysis of antigen-presenting cells. KEYWORDS: 3D-structure;ADP-ribosylation;Cell membrane;Disulfide bond;Glycoprotein;Ion channel;Ion transport;Ligand-gated ion channel;Lipoprotein;Membrane;Palmitate;Phosphoprotein;Receptor;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein. Q64732,"PROTEIN NAMES: Forkhead box protein B1 (Transcription factor FKH-5) ORGANISM: Mus musculus (Mouse) FUNCTION: Transcription factor expressed by neural progenitor cells in specific regions of the embryonic neuroepithelium. Essential for the mammillary nuclei maintenance. Negatively regulates the proliferation of oligodendrocyte progenitors and promotes oligodendrocyte maturation. Also expressed in mammary glands, plays a role in lactation, controls development of mammary glands and the inferior colliculi of the midbrain in the central nervous system that regulates the milk-ejection reflex. KEYWORDS: DNA-binding;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " Q653T6,"PROTEIN NAMES: Solanesyl-diphosphate synthase 1, mitochondrial (OsSPS1) (All-trans-nonaprenyl-diphosphate synthase 1 (geranyl-diphosphate specific)) PROTEIN FAMILY: FPP/GGPP synthase family ORGANISM: Oryza sativa subsp. japonica (Rice) SIMILARITY: Belongs to the FPP/GGPP synthase family. FUNCTION: Involved in the supply of solanesyl diphosphate for ubiquinone-9 (UQ-9) biosynthesis in mitochondria. Farnesyl diphosphate is the preferred substrate. PATHWAY: Cofactor biosynthesis; ubiquinone biosynthesis. KEYWORDS: Isoprene biosynthesis;Magnesium;Metal-binding;Mitochondrion;Reference proteome;Transferase;Transit peptide SUBCELLULAR LOCATION: Mitochondrion " Q65Z91,"PROTEIN NAMES: Tsukushi (C-TSK) (Leucine-rich repeat-containing protein 54) ORGANISM: Gallus gallus (Chicken) FUNCTION: Contributes to various developmental events through its interactions with multiple signaling pathways. Dorsalizing factor involved in the induction of Hensen's node by inhibiting bone morphogenetic proteins during gastrulation and by enhancing DVR1/VG1 activity. Wnt signaling inhibitor which competes with WNT2B for binding to Wnt receptor FZD4 and represses WNT2B-dependent development of the peripheral eye.; FUNCTION: [Isoform 1]: Shows strong bone morphogenetic protein antagonistic activity.; FUNCTION: [Isoform 2]: Shows weak bone morphogenetic protein antagonistic activity. KEYWORDS: Alternative splicing;Developmental protein;Glycoprotein;Leucine-rich repeat;Reference proteome;Repeat;Secreted;Signal SUBCELLULAR LOCATION: Secreted MISCELLANEOUS: This factor is named 'Tsukushi' because its expression pattern in chick embryos is similar to the shape of the Japanese horsetail plant, tsukushi." Q66GI4,"PROTEIN NAMES: Proteinaceous RNase P 1, chloroplastic/mitochondrial (Pentatricopeptide repeat-containing protein At2g32230) PROTEIN FAMILY: PPR family, P subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the PPR family. P subfamily. FUNCTION: Endonuclease RNase P responsible for the 5' maturation of tRNA precursors. Preferentially cleaves at the unusual cleavage site, but also able to cleave at the classical cleavage site. Also involved in the maturation of mRNAs in mitochondria. KEYWORDS: 3D-structure;Chloroplast;Hydrolase;Magnesium;Manganese;Metal-binding;Mitochondrion;Nuclease;Plastid;Reference proteome;Repeat;Transit peptide;tRNA processing;Zinc SUBCELLULAR LOCATION: Mitochondrion Plastid, chloroplast " Q66GP9,"PROTEIN NAMES: NO-associated protein 1, chloroplastic/mitochondrial (AtNOA1) (Dubious mitochondrial nitric oxide synthase 1) (AtNOS1) (GTPase NOA1) (Protein RESISTANT TO INHIBITION BY FOSMIDOMYCIN 1) PROTEIN FAMILY: TRAFAC class YlqF/YawG GTPase family, NOA1 subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the TRAFAC class YlqF/YawG GTPase family. NOA1 subfamily. FUNCTION: Exhibits cGTPase activity; binds and hydrolyzes specifically GTP. May participate in ribosome assembly and stability and thus regulates protein synthesis in chloroplasts. The GTPase activity requires MgCl(2)and the presence of either KCl or (NH(4))(2)SO(4). Involved in the post-transcriptional regulation of the methylerythritol phosphate (MEP) pathway. Involved in chlorophyll-a fluorescence regulation.; FUNCTION: May mediate the production or accumulation of nitric oxide (NO) which is a messenger molecule involved in hormonal signaling and defense responses in plant. Acts as an antisenescence agent. Plays a crucial role in both extracellular calmodulin (ExtCaM)-triggered and salicylic acid (SA)-mediated H(2)O(2)-dependent stomatal closure. KEYWORDS: Alternative splicing;Chloroplast;GTP-binding;Hydrolase;Mitochondrion;NADP;Nucleotide-binding;Oxidoreductase;Plastid;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Mitochondrion Plastid, chloroplast Note=Was initially thought to be mitochondrial. In fact seems to be chloroplastic. " Q66HC3,"PROTEIN NAMES: Guanine nucleotide exchange factor C9orf72 homolog ORGANISM: Rattus norvegicus (Rat) FUNCTION: Component of the C9orf72-SMCR8 complex, a complex that has guanine nucleotide exchange factor (GEF) activity and regulates autophagy. In the complex, C9orf72 and SMCR8 probably constitute the catalytic subunits that promote the exchange of GDP to GTP, converting inactive GDP-bound RAB8A and RAB39B into their active GTP-bound form, thereby promoting autophagosome maturation. The C9orf72-SMCR8 complex also acts as a regulator of autophagy initiation by interacting with the ULK1/ATG1 kinase complex and modulating its protein kinase activity. As part of the C9orf72-SMCR8 complex, stimulates RAB8A and RAB11A GTPase activity in vitro (By similarity). Positively regulates initiation of autophagy by regulating the RAB1A-dependent trafficking of the ULK1/ATG1 kinase complex to the phagophore which leads to autophagosome formation. Acts as a regulator of mTORC1 signaling by promoting phosphorylation of mTORC1 substrates. Plays a role in endosomal trafficking. May be involved in regulating the maturation of phagosomes to lysosomes. Promotes the lysosomal localization and lysosome-mediated degradation of CARM1 which leads to inhibition of starvation-induced lipid metabolism (By similarity). Regulates actin dynamics in motor neurons by inhibiting the GTP-binding activity of ARF6, leading to ARF6 inactivation. This reduces the activity of the LIMK1 and LIMK2 kinases which are responsible for phosphorylation and inactivation of CFL1/cofilin, leading to cofilin activation. Positively regulates axon extension and axon growth cone size in spinal motor neurons. Required for SMCR8 protein expression and localization at pre- and post-synaptic compartments in the forebrain, also regulates protein abundance of RAB3A and GRIA1/GLUR1 in post-synaptic compartments in the forebrain and hippocampus (By similarity). Plays a role within the hematopoietic system in restricting inflammation and the development of autoimmunity. KEYWORDS: Autophagy;Cell projection;Cytoplasm;Cytoplasmic vesicle;Endosome;Guanine-nucleotide releasing factor;Lysosome;Nucleus;Reference proteome;Secreted SUBCELLULAR LOCATION: Nucleus Cytoplasm Cytoplasm, P-body Cytoplasm, Stress granule Endosome Lysosome Cytoplasmic vesicle, autophagosome Secreted Cell projection, axon Cell projection, growth cone Perikaryon Note=Detected in the cytoplasm of neurons from brain tissue. Detected in the nucleus in fibroblasts. Associates with cytoplasmic stress granules following cellular stress. During corticogenesis, transitions from being predominantly cytoplasmic to a more even nucleocytoplasmic distribution. " Q66HG9,"PROTEIN NAMES: Mitochondrial antiviral-signaling protein (MAVS) (Interferon beta promoter stimulator protein 1) (IPS-1) (Virus-induced-signaling adapter) (VISA) ORGANISM: Rattus norvegicus (Rat) FUNCTION: Adapter required for innate immune defense against viruses. Acts downstream of DHX33, RIGI and IFIH1/MDA5, which detect intracellular dsRNA produced during viral replication, to coordinate pathways leading to the activation of NF-kappa-B, IRF3 and IRF7, and to the subsequent induction of antiviral cytokines such as IFN-beta and RANTES (CCL5). Peroxisomal and mitochondrial MAVS act sequentially to create an antiviral cellular state. Upon viral infection, peroxisomal MAVS induces the rapid interferon-independent expression of defense factors that provide short-term protection, whereas mitochondrial MAVS activates an interferon-dependent signaling pathway with delayed kinetics, which amplifies and stabilizes the antiviral response. May activate the same pathways following detection of extracellular dsRNA by TLR3. May protect cells from apoptosis. Involved in NLRP3 inflammasome activation by mediating NLRP3 recruitment to mitochondria. KEYWORDS: Antiviral defense;Host-virus interaction;Immunity;Innate immunity;Isopeptide bond;Lipoprotein;Membrane;Methylation;Mitochondrion;Mitochondrion outer membrane;Palmitate;Peroxisome;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix;Ubl conjugation SUBCELLULAR LOCATION: Mitochondrion outer membrane ; Single-pass membrane protein Mitochondrion Peroxisome " Q66I08,"PROTEIN NAMES: Organic solute transporter subunit alpha (OST-alpha) (Solute carrier family 51 subunit alpha) PROTEIN FAMILY: OST-alpha family ORGANISM: Danio rerio (Zebrafish) (Brachydanio rerio) SIMILARITY: Belongs to the OST-alpha family. FUNCTION: Essential component of the Ost-alpha/Ost-beta complex, a heterodimer that acts as the intestinal basolateral transporter responsible for the translocation of bile acids (such as taurocholate), steroids (such as estrone sulfate), and eicosanoids (such as prostaglandin E2). KEYWORDS: Cell membrane;Endoplasmic reticulum;Glycoprotein;Lipid transport;Membrane;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Endoplasmic reticulum membrane; Multi-pass membrane protein. " Q66PF4,"PROTEIN NAMES: Cinnamate beta-D-glucosyltransferase (UDP-glucose:cinnamate glucosyltransferase) (FaGT2) PROTEIN FAMILY: UDP-glycosyltransferase family ORGANISM: Fragaria ananassa (Strawberry) (Fragaria chiloensis x Fragaria virginiana) SIMILARITY: Belongs to the UDP-glycosyltransferase family. FUNCTION: Broad spectrum multifunctional glucosyltransferase. Catalyzes the formation of cinnamic acid and p-coumaric acid glucose esters during fruit ripening. Accepted substrates range from derivatives of cinnamic acid and benzoic acid to heterocyclic and aliphatic compounds, resulting in the formation of O- and S-glucose esters and O-glucosides. May also be involved in detoxification of xenobiotics. KEYWORDS: Detoxification;Glycosyltransferase;Transferase " Q66PG2,"PROTEIN NAMES: Xylosyl- and glucuronyltransferase LARGE1 (Acetylglucosaminyltransferase-like 1A) (Glycosyltransferase-like protein) (LARGE xylosyl- and glucuronyltransferase 1) [Includes: Alpha-1,3-xylosyltransferase LARGE1 ; Beta-1,3-glucuronyltransferase LARGE1 ] PROTEIN FAMILY: Glycosyltransferase 49 family; Glycosyltransferase 8 family ORGANISM: Danio rerio (Zebrafish) (Brachydanio rerio) SIMILARITY: In the C-terminal section; belongs to the glycosyltransferase 49 family.; SIMILARITY: In the N-terminal section; belongs to the glycosyltransferase 8 family. FUNCTION: Bifunctional glycosyltransferase with both alpha-1,3-xylosyltransferase and beta-1,3-glucuronyltransferase activities involved in the maturation of alpha-dystroglycan (DAG1) by glycosylation leading to DAG1 binding to laminin G-like domain-containing extracellular proteins with high affinity. Elongates the glucuronyl-beta-1,4-xylose-beta disaccharide primer structure initiated by B4GAT1 by adding repeating units [-3-Xylose-alpha-1,3-GlcA-beta-1-] to produce a heteropolysaccharide. Requires the phosphorylation of core M3 (O-mannosyl trisaccharide) by POMK to elongate the glucuronyl-beta-1,4-xylose-beta disaccharide primer (By similarity). Plays a key role in skeletal muscle function and regeneration (By similarity). PATHWAY: Protein modification; protein glycosylation. KEYWORDS: Coiled coil;Glycoprotein;Glycosyltransferase;Golgi apparatus;Manganese;Membrane;Metal-binding;Multifunctional enzyme;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus membrane ; Single-pass type II membrane protein " Q67EQ0,"PROTEIN NAMES: C-type lectin domain family 4 member A (C-type lectin superfamily member 6) (Dendritic cell inhibitory receptor) (CD antigen CD367) ORGANISM: Rattus norvegicus (Rat) FUNCTION: C-type lectin receptor that binds carbohydrates mannose and fucose but also weakly interacts with N-acetylglucosamine (GlcNAc) in a Ca(2+)-dependent manner. Involved in regulating immune reactivity. Once triggered by antigen, it is internalized by clathrin-dependent endocytosis and delivers its antigenic cargo into the antigen presentation pathway resulting in cross-priming of CD8(+) T cells. This cross-presentation and cross-priming are enhanced by TLR7 and TLR8 agonists with increased expansion of the CD8(+) T cells, high production of IFNG and TNF with reduced levels of IL4, IL5 and IL13. In plasmacytoid dendritic cells, inhibits TLR9-mediated IFNA and TNF production. May be involved via its ITIM motif (immunoreceptor tyrosine-based inhibitory motifs) in the inhibition of B-cell-receptor-mediated calcium mobilization and protein tyrosine phosphorylation. KEYWORDS: Adaptive immunity;Calcium;Cell membrane;Disulfide bond;Glycoprotein;Immunity;Innate immunity;Lectin;Membrane;Metal-binding;Reference proteome;Signal-anchor;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type II membrane protein ; Extracellular side " Q67XG0,"PROTEIN NAMES: Two-on-two hemoglobin-3 (AtGLB3) (2-on-2 hemoglobin-3) PROTEIN FAMILY: Truncated hemoglobin family, Group II subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the truncated hemoglobin family. Group II subfamily. FUNCTION: Hemoglobin-like protein that exhibits an unusual concentration-independent binding of O(2) and CO. May promote shoot organogenesis from root explants in vitro. Inhibits RGLG3 and RGLG4 ubiquitination activity. KEYWORDS: 3D-structure;Heme;Iron;Metal-binding;Oxygen transport;Reference proteome;Transport " Q67XT3,"PROTEIN NAMES: Kinetochore protein SPC24 homolog (AtSPC24) (Protein MERISTEM UNSTRUCTURED) PROTEIN FAMILY: SPC24 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the SPC24 family. FUNCTION: Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity to ensure proper cell division. Required for the maintenance of plant architecture. KEYWORDS: Cell cycle;Cell division;Centromere;Chromosome;Coiled coil;Mitosis;Reference proteome SUBCELLULAR LOCATION: Chromosome, centromere Note=Colocalizes with CENH3 at the centromere. " Q67Y99,"PROTEIN NAMES: Clp protease adapter protein ClpF, chloroplastic ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Clp protease adapter that facilitates CLPS1 recruitment to ClpC chaperones thus forming a binary adapter for selective substrate recognition and delivery to plastid Clp protease system (CLPC). KEYWORDS: Alternative splicing;Chloroplast;Coiled coil;Plastid;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast " Q680Q4,"PROTEIN NAMES: E3 SUMO-protein ligase SIZ1 (E3 SUMO-protein transferase SIZ1) PROTEIN FAMILY: PIAS family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the PIAS family. FUNCTION: E3 SUMO protein ligase involved in regulation processes. Mediates SUMO/ attachment to PHR1, a MYB transcriptional activator controlling the phosphate deficiency responses. Functions as an upstream negative regulator of salicylic acid (SA) accumulation and subsequent SA-mediated systemic acquired resistance (SAR) signaling. Probably not involved in jasmonic acid (JA)-mediated defense response. Participates in abiotic stress-induced sumoylation. Controls heat shock-induced SUMO1 and SUMO2 conjugation and facilitates basal thermotolerance. Involved in freezing tolerance by mediating sumoylation of ICE1, a transcription activator of the cold signaling regulator CBF3/DREB1A. Acts as a positive regulator of drought stress tolerance. Acts as a floral repressor that promotes FLC expression by repressing FLD activity through sumoylation. Acts as a negative regulator of abscisic acid (ABA) signaling through ABI5 sumoylation. Mediates sumoylation of SCE1, GTE3 and GTE5. Functions as a negative regulator of SnRK1 signaling through sumoylation of several components of the SnRK1 complex. PATHWAY: Protein modification; protein sumoylation. KEYWORDS: 3D-structure;Alternative splicing;Flowering;Isopeptide bond;Metal-binding;Nucleus;Plant defense;Reference proteome;Stress response;Transferase;Ubl conjugation;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus speckle " Q682S0,PROTEIN NAMES: Root phototropism protein 2 (BTB/POZ domain-containing protein RPT2) PROTEIN FAMILY: NPH3 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the NPH3 family. FUNCTION: May act as a substrate-specific adapter of an E3 ubiquitin-protein ligase complex (CUL3-RBX1-BTB) which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (By similarity). Signal transducer of the phototropic response and photo-induced movements. Necessary for root phototropism. Involved in hypocotyl phototropism under high rate but not under low rate light. Regulates stomata opening. Seems to be not involved in chloroplast accumulation and translocation. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Alternative splicing;Phosphoprotein;Reference proteome;Transducer;Ubl conjugation pathway Q682U6,"PROTEIN NAMES: Exonuclease DPD1, chloroplastic/mitochondrial (Mg2+-dependent DNA exonuclease) (Protein DEFECTIVE IN POLLEN DNA DEGRADATION 1) PROTEIN FAMILY: Exonuclease superfamily, TREX family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the exonuclease superfamily. TREX family. FUNCTION: Exonuclease required for organelle DNA degradation during pollen development. Plays non-essential roles in maternal inheritance. May be part of the DNA salvage machinery. KEYWORDS: Chloroplast;Exonuclease;Hydrolase;Magnesium;Metal-binding;Mitochondrion;Nuclease;Plastid;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast Mitochondrion MISCELLANEOUS: DPD1 homologs are present in flowering plants but not in moss, green algae and animals." Q68CP4,"PROTEIN NAMES: Heparan-alpha-glucosaminide N-acetyltransferase (Transmembrane protein 76) ORGANISM: Homo sapiens (Human) FUNCTION: Lysosomal acetyltransferase that acetylates the non-reducing terminal alpha-glucosamine residue of intralysosomal heparin or heparan sulfate, converting it into a substrate for luminal alpha-N-acetyl glucosaminidase. KEYWORDS: 3D-structure;Acyltransferase;Alternative initiation;Disease variant;Disulfide bond;Glycoprotein;Lysosome;Membrane;Mucopolysaccharidosis;Phosphoprotein;Reference proteome;Retinitis pigmentosa;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Lysosome membrane ; Multi-pass membrane protein Note=Colocalizes with the lysosomal marker LAMP2. The signal peptide is not cleaved upon translocation into the endoplasmic reticulum; the precursor is probably targeted to the lysosomes via the adapter protein complex-mediated pathway that involves tyrosine- and/or dileucine-based conserved amino acid motifs in the last C-terminus 16-amino acid domain. MISCELLANEOUS: A signal sequence is predicted but has been shown not to be cleaved in the reticulum endoplasmic.; MISCELLANEOUS: [Isoform 1]: Intralysosomal proteolytic cleavage is faster and enzymatic activity higher than isoform 2." Q68CZ6,"PROTEIN NAMES: HAUS augmin-like complex subunit 3 PROTEIN FAMILY: HAUS3 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the HAUS3 family. FUNCTION: Contributes to mitotic spindle assembly, maintenance of centrosome integrity and completion of cytokinesis as part of the HAUS augmin-like complex. KEYWORDS: 3D-structure;Acetylation;Alternative splicing;Cell cycle;Cell division;Coiled coil;Cytoplasm;Cytoskeleton;Microtubule;Mitosis;Reference proteome SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Cytoplasm, cytoskeleton, spindle Note=Localizes to interphase centrosomes and to mitotic spindle microtubules. " Q68EM7,"PROTEIN NAMES: Rho GTPase-activating protein 17 (Rho-type GTPase-activating protein 17) (RhoGAP interacting with CIP4 homologs protein 1) (RICH-1) ORGANISM: Homo sapiens (Human) FUNCTION: Rho GTPase-activating protein involved in the maintenance of tight junction by regulating the activity of CDC42, thereby playing a central role in apical polarity of epithelial cells. Specifically acts as a GTPase activator for the CDC42 GTPase by converting it to an inactive GDP-bound state. The complex formed with AMOT acts by regulating the uptake of polarity proteins at tight junctions, possibly by deciding whether tight junction transmembrane proteins are recycled back to the plasma membrane or sent elsewhere. Participates in the Ca(2+)-dependent regulation of exocytosis, possibly by catalyzing GTPase activity of Rho family proteins and by inducing the reorganization of the cortical actin filaments. Acts as a GTPase activator in vitro for RAC1. KEYWORDS: Alternative splicing;Cell junction;Cytoplasm;GTPase activation;Membrane;Phosphoprotein;Reference proteome;SH3-binding;Tight junction SUBCELLULAR LOCATION: Membrane; Peripheral membrane protein. Cytoplasm. Cell junction, tight junction. Note=Associates with membranes and concentrates at sites of cell-cell contact. " Q68FF6,"PROTEIN NAMES: ARF GTPase-activating protein GIT1 (ARF GAP GIT1) (G protein-coupled receptor kinase-interactor 1) (GRK-interacting protein 1) ORGANISM: Mus musculus (Mouse) FUNCTION: GTPase-activating protein for ADP ribosylation factor family members, including ARF1. Multidomain scaffold protein that interacts with numerous proteins and therefore participates in many cellular functions, including receptor internalization, focal adhesion remodeling, and signaling by both G protein-coupled receptors and tyrosine kinase receptors (By similarity). Through PAK1 activation, positively regulates microtubule nucleation during interphase. Plays a role in the regulation of cytokinesis; for this function, may act in a pathway also involving ENTR1 and PTPN13 (By similarity). May promote cell motility both by regulating focal complex dynamics and by the activation of RAC1 (By similarity). May act as scaffold for MAPK1/3 signal transduction, recruiting MAPK1/3 to focal adhesions after EGF stimulation via a Src-dependent pathway, hence stimulating cell migration (By similarity). Plays a role in brain development and function. Involved in the regulation of spine density and synaptic plasticity that is required for processes involved in learning. Plays an important role in dendritic spine morphogenesis and synapse formation. In hippocampal neurons, recruits guanine nucleotide exchange factors (GEFs), such as ARHGEF7/beta-PIX, to the synaptic membrane. These in turn locally activate RAC1, which is an essential step for spine morphogenesis and synapse formation. May contribute to the organization of presynaptic active zones through oligomerization and formation of a Piccolo/PCLO-based protein network, which includes ARHGEF7/beta-PIX and FAK1 (By similarity). In neurons, through its interaction with liprin-alpha family members, may be required for AMPA receptor (GRIA2/3) proper targeting to the cell membrane (By similarity). In complex with GABA(A) receptors and ARHGEF7, plays a crucial role in regulating GABA(A) receptor synaptic stability, maintaining GPHN/gephyrin scaffolds and hence GABAergic inhibitory synaptic transmission, by locally coordinating RAC1 and PAK1 downstream effector activity, leading to F-actin stabilization (By similarity). May also be important for RAC1 downstream signaling pathway through PAK3 and regulation of neuronal inhibitory transmission at presynaptic input. Required for successful bone regeneration during fracture healing. The function in intramembranous ossification may, at least partly, exerted by macrophages in which GIT1 is a key negative regulator of redox homeostasis, IL1B production, and glycolysis, acting through the ERK1/2/NRF2/NFE2L2 axis. May play a role in angiogenesis during fracture healing. In this process, may regulate activation of the canonical NF-kappa-B signal in bone mesenchymal stem cells by enhancing the interaction between NEMO and 'Lys-63'-ubiquitinated RIPK1/RIP1, eventually leading to enhanced production of VEGFA and others angiogenic factors (By similarity). Essential for VEGF signaling through the activation of phospholipase C-gamma and ERK1/2, hence may control endothelial cell proliferation and angiogenesis. KEYWORDS: 3D-structure;ANK repeat;Cell junction;Cell projection;Coiled coil;Cytoplasm;Cytoskeleton;GTPase activation;Metal-binding;Phosphoprotein;Reference proteome;Repeat;Synapse;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cytoplasm Presynapse Postsynapse Postsynaptic density Cell junction, focal adhesion Cell projection, lamellipodium Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Cytoplasm, cytoskeleton, spindle pole Note=Cycles between at least 3 distinct intracellular compartments, including focal adhesions, cytosolic complexes, containing at least PXN/paxillin, ARHGEF7 and PAK1, and membrane protrusions. During cell migration, moves from the disassembling adhesions into the cytosol and towards the leading edge. In adherent cells, localizes to adhesions. Recruitment to adhesions may be mediated by RAC1 and active tyrosine-phosphorylated PXN (By similarity). May be present in both excitatory and inhibitory synapses. In hippocampal neurons, recruitment of GIT1 to synapses is regulated by ephrinB activation and ephrinB downstream effector GRB4/NCK2. In hippocampal neurons, partially colocalizes with PCLO (By similarity). Interaction with GRIN3A limits GIT1 synaptic localization (By similarity). Localization to the centrosome does not depend upon the presence of gamma-tubulin (By similarity). " Q68SN8,PROTEIN NAMES: Fc receptor-like protein 5 (FcR-like protein 5) (FcRL5) (BXMAS1-like protein 2) (mBXMH2) (Fc receptor homolog 3) (FcRH3) (moFcRH3) (CD antigen CD307e) ORGANISM: Mus musculus (Mouse) FUNCTION: May play a role in fertilization. KEYWORDS: Alternative splicing;Cell membrane;Disulfide bond;Fertilization;Glycoprotein;Immunoglobulin domain;Membrane;Phosphoprotein;Receptor;Reference proteome;Repeat;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein Q69SV0,"PROTEIN NAMES: Probable L-ascorbate peroxidase 8, chloroplastic (OsAPx8) PROTEIN FAMILY: Peroxidase family, Ascorbate peroxidase subfamily ORGANISM: Oryza sativa subsp. japonica (Rice) SIMILARITY: Belongs to the peroxidase family. Ascorbate peroxidase subfamily. FUNCTION: Involved in defense response and tolerance to the bacterial pathogen Xanthomonas oryzae pv. oryzae (Xoo). Plays an important role in hydrogen peroxide removal during infection by Xoo. Involved in response to abiotic stress. Plays a role in hydrogen peroxide removal durings salt stress. KEYWORDS: Calcium;Chloroplast;Heme;Hydrogen peroxide;Iron;Membrane;Metal-binding;Oxidoreductase;Peroxidase;Plant defense;Plastid;Potassium;Reference proteome;Stress response;Thylakoid;Transit peptide;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane ; Single-pass membrane protein MISCELLANEOUS: Binds one cation per subunit; probably K(+), but might also be Ca(2+) (By similarity). Plants over-expressing APX8 exhibit increased tolerance to the bacterial pathogen Xanthomonas oryzae pv. oryzae (Xoo). Plants silencing APX8 show increased sensitivity to Xoo." Q69Z69,"PROTEIN NAMES: N-acetyltransferase ESCO1 (Establishment of cohesion 1 homolog 1) PROTEIN FAMILY: Acetyltransferase family, ECO subfamily ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the acetyltransferase family. ECO subfamily. FUNCTION: Acetyltransferase required for the establishment of sister chromatid cohesion. Couples the processes of cohesion and DNA replication to ensure that only sister chromatids become paired together. In contrast to the structural cohesins, the deposition and establishment factors are required only during S phase. Acts by mediating the acetylation of cohesin component SMC3. KEYWORDS: Acyltransferase;Alternative splicing;Cell cycle;Chromosome;Isopeptide bond;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Transferase;Ubl conjugation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Chromosome Note=Nuclear at interphase, associated with chromosomes during mitosis. " Q69ZQ1,"PROTEIN NAMES: Myogenesis-regulating glycosidase (Nuclear envelope transmembrane protein 37) (Uncharacterized family 31 glucosidase KIAA1161) PROTEIN FAMILY: Glycosyl hydrolase 31 family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the glycosyl hydrolase 31 family. FUNCTION: Putative glycosidase. Promotes myogenesis by activating AKT signaling through the maturation and secretion of IGF2. KEYWORDS: Endoplasmic reticulum;Glycoprotein;Glycosidase;Hydrolase;Membrane;Nucleus;Reference proteome;Signal-anchor;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Nucleus membrane ; Single-pass type II membrane protein Endoplasmic reticulum membrane ; Single-pass type II membrane protein Note=Only a minor fraction is present in the peripheral endoplasmic reticulum. MISCELLANEOUS: The requirement of the predicted catalytic residue Asp-462 to support myogenic function strongly suggests that MYORG is an enzymatically active glycosidase in vivo, even if concrete experimental proof for enzymatic activity is still missing." Q6A2H4,"PROTEIN NAMES: Interleukin-10 (IL-10) PROTEIN FAMILY: IL-10 family ORGANISM: Gallus gallus (Chicken) SIMILARITY: Belongs to the IL-10 family. FUNCTION: Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation (By similarity). In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling (By similarity). KEYWORDS: Cytokine;Disulfide bond;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted MISCELLANEOUS: Chickens susceptible to E.maxima infection show higher constitutive and post-infection levels in spleen and higher post-infection levels in small intestine than infection-resistant animals." Q6AY25,"PROTEIN NAMES: Transmembrane emp24 domain-containing protein 3 (p24 family protein gamma-4) (p24gamma4) PROTEIN FAMILY: EMP24/GP25L family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the EMP24/GP25L family. FUNCTION: Potential role in vesicular protein trafficking, mainly in the early secretory pathway. Contributes to the coupled localization of TMED2 and TMED10 in the cis-Golgi network (By similarity). KEYWORDS: Cytoplasmic vesicle;Endoplasmic reticulum;Golgi apparatus;Membrane;Methylation;Protein transport;Reference proteome;Signal;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Endoplasmic reticulum-Golgi intermediate compartment membrane ; Single-pass type I membrane protein Golgi apparatus, cis-Golgi network membrane ; Single-pass type I membrane protein Golgi apparatus, Golgi stack membrane ; Single-pass type I membrane protein Endoplasmic reticulum membrane ; Single-pass type I membrane protein Cytoplasmic vesicle, COPI-coated vesicle membrane ; Single-pass type I membrane protein Note=Probably cycles between compartments of the early secretatory pathway. " Q6AYC2,"PROTEIN NAMES: Immunity-related GTPase family M protein PROTEIN FAMILY: TRAFAC class dynamin-like GTPase superfamily, IRG family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the TRAFAC class dynamin-like GTPase superfamily. IRG family. FUNCTION: Immunity-related GTPase that plays important roles in innate immunity and inflammatory response (By similarity). Acts as a dynamin-like protein that binds to intracellular membranes and promotes remodeling and trafficking of those membranes (By similarity). Required for clearance of acute protozoan and bacterial infections by interacting with autophagy and lysosome regulatory proteins, thereby promoting the fusion of phagosomes with lysosomes for efficient degradation of cargo including microbes (By similarity). Regulates selective autophagy, including xenophagy and mitophagy, both directly and indirectly (By similarity). Directly regulates autophagy by acting as a molecular adapter that promotes the coassembly of the core autophagy machinery to mediate antimicrobial defense: Irgm (1) activates AMPK, which in turn phosphorylates ULK1 and BECN1 to induce autophagy, (2) promotes the coassembly of ULK1 and BECN1, enhancing BECN1-interacting partners and (3) influences the composition of the BECN1 complex, by competing with the negative regulators BCL2 and RUBCN, to trigger autophagy (By similarity). Also activates autophagy by promoting recruitment of STX17 to autophagosomes (By similarity). In collaboration with ATG8 proteins, regulate lysosomal biogenesis, a fundamental process for any autophagic pathway, by promoting TFEB dephosphorylation (By similarity). Also modulates autophagy by assisting with autophagosome formation and preventing lysosomal deacidification (By similarity). Regulates autophagy by affecting mitochondrial fusion and fission (By similarity). Also involved in M1 macrophage activation for the production of proinflammatory cytokines (By similarity). While activating autophagy, acts as a key negative regulator of the inflammatory and interferon responses both by (1) promoting mitophagy and (2) mediating autophagy-dependent degradation of effectors of the inflammatory response (By similarity). Promotes degradation of damaged and IFNG/IFN-gamma-stressed mitochondria via mitophagy, preventing cytosolic release of ligands that activate inflammation (By similarity). Negatively regulates interferon-signaling in hematopoietic stem cells, preserving hematopoietic stem cell number and function (By similarity). Promotes expansion of activated CD4(+) T-cells by inhibiting IFNG/IFN-gamma signaling, thereby preventing Ifng-mediated cell death of CD4(+) T-cells (By similarity). Acts as a suppressor of inflammation by promoting recruitment of inflammation effectors, such as CGAS, RIGI/RIG-I and NLRP3, to autophagosome membranes, leading to their SQSTM1/p62-dependent autophagic degradation (By similarity). Also directly inhibits assembly of the NLRP3 inflammasome by preventing the association between NLRP3 and PYCARD (By similarity). Acts as a negative regulator of antiviral innate immune response by suppressing the RIPK2-dependent pro-inflammatory response: mediates recruitment of RIPosomes, composed of RIPK2 and NOD1 or NOD2, to autophagosome membranes, promoting their SQSTM1/p62-dependent autophagic degradation (By similarity). KEYWORDS: Autophagy;Cell membrane;Cell projection;Cytoplasmic vesicle;Endosome;Golgi apparatus;GTP-binding;Hydrolase;Immunity;Innate immunity;Isopeptide bond;Lipid-binding;Lipoprotein;Lysosome;Membrane;Mitochondrion;Nucleotide-binding;Palmitate;Phosphoprotein;Reference proteome;Ubl conjugation SUBCELLULAR LOCATION: Golgi apparatus membrane Cell membrane Cytoplasmic vesicle, phagosome membrane Cytoplasmic vesicle, autophagosome membrane Lysosome membrane Late endosome membrane Mitochondrion membrane Cell projection, phagocytic cup Note=Behaves like an integral membrane protein. Recruited to the plasma membrane around forming phagocytic cups, it remains associated with maturing phagosomes. Association with phagosomes is dependent on nucleotide-binding but is IFNG-independent. Also detected in late endosomes and lysosomes: lysosomal localization is IFN-gamma-induced during bacterial infections such as S.typhimurium infection. " Q6AZZ1,"PROTEIN NAMES: E3 ubiquitin-protein ligase TRIM68 (RING finger protein 137) (RING-type E3 ubiquitin transferase TRIM68) (SSA protein SS-56) (SS-56) (Tripartite motif-containing protein 68) PROTEIN FAMILY: TRIM/RBCC family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the TRIM/RBCC family. FUNCTION: Functions as a ubiquitin E3 ligase. Acts as a coactivator of androgen receptor (AR) depending on its ubiquitin ligase activity. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Alternative splicing;Coiled coil;Cytoplasm;Metal-binding;Nucleus;Reference proteome;Transferase;Ubl conjugation;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cytoplasm, perinuclear region. Nucleus. Note=Colocalized with AR in nucleus. MISCELLANEOUS: Antibodies against TRIM68 are found in patients with systemic lupus erythematosus (SLE) and primary Sjoegren syndrome." Q6BDI9,PROTEIN NAMES: Rab15 effector protein ORGANISM: Homo sapiens (Human) FUNCTION: Regulates transferrin receptor recycling from the endocytic recycling compartment. KEYWORDS: 3D-structure;Endosome;Lipoprotein;Membrane;Myristate;Reference proteome SUBCELLULAR LOCATION: Early endosome membrane Note=Colocalizes with RAB11 and RAB15 to the endocytic recycling compartment. Q6BF16,"PROTEIN NAMES: 2-dehydro-3-deoxy-6-phosphogalactonate aldolase (2-oxo-3-deoxygalactonate 6-phosphate aldolase) (6-phospho-2-dehydro-3-deoxygalactonate aldolase) (6-phospho-2-keto-3-deoxygalactonate aldolase) (KDPGal) PROTEIN FAMILY: KHG/KDPG aldolase family ORGANISM: Escherichia coli (strain K12) SIMILARITY: Belongs to the KHG/KDPG aldolase family. FUNCTION: Involved in the degradation of galactose via the DeLey-Doudoroff pathway. Catalyzes the reversible, stereospecific retro-aldol cleavage of 2-keto-3-deoxy-6-phosphogalactonate (KDPGal) to pyruvate and D-glyceraldehyde-3-phosphate. In the synthetic direction, it catalyzes the addition of pyruvate to electrophilic aldehydes with re-facial selectivity. It can use a limited number of aldehyde substrates, including D-glyceraldehyde-3-phosphate (natural substrate), D-glyceraldehyde, glycolaldehyde, 2-pyridinecarboxaldehyde, D-ribose, D-erythrose and D-threose. It efficiently catalyzes aldol addition only using pyruvate as the nucleophilic component and accepts both stereochemical configurations at C2 of the electrophile. PATHWAY: Carbohydrate acid metabolism; D-galactonate degradation; D-glyceraldehyde 3-phosphate and pyruvate from D-galactonate: step 3/3. KEYWORDS: 3D-structure;Lyase;Reference proteome " Q6C4A3,"PROTEIN NAMES: Decapping nuclease RAI1 (NAD-capped RNA hydrolase RAI1) (DeNADding enzyme RAI1) PROTEIN FAMILY: DXO/Dom3Z family ORGANISM: Yarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica) SIMILARITY: Belongs to the DXO/Dom3Z family. FUNCTION: Decapping enzyme for NAD-capped RNAs: specifically hydrolyzes the nicotinamide adenine dinucleotide (NAD) cap from a subset of RNAs by removing the entire NAD moiety from the 5'-end of an NAD-capped RNA (By similarity). The NAD-cap is present at the 5'-end of some RNAs and snoRNAs. In contrast to the canonical 5'-end N7 methylguanosine (m7G) cap, the NAD cap promotes mRNA decay (By similarity). Also acts as a non-canonical decapping enzyme that removes the entire cap structure of m7G capped or incompletely capped RNAs (By similarity). Has decapping activity toward incomplete 5'-end m7G cap mRNAs such as unmethylated 5'-end-capped RNA (cap0), while it has no activity toward 2'-O-ribose methylated m7G cap (cap1) (By similarity). Also possesses RNA 5'-pyrophosphohydrolase activity by hydrolyzing the 5'-end triphosphate to release pyrophosphates (By similarity). Stimulates exoribonuclease activity of Rat1, allowing it to degrade RNAs with stable secondary structure more effectively (By similarity). KEYWORDS: Hydrolase;Metal-binding;mRNA processing;Nuclease;Nucleotide-binding;Nucleus;Reference proteome;RNA-binding SUBCELLULAR LOCATION: Nucleus " Q6DBG8,"PROTEIN NAMES: Probable arabinosyltransferase ARAD1 (Arabinan alpha-1,5-arabinosyltransferase) (L-Arabinosyltransferase) (Protein ARABINAN DEFICIENT 1) PROTEIN FAMILY: Glycosyltransferase 47 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the glycosyltransferase 47 family. FUNCTION: Probable arabinosyl transferase responsible for the polymerization of arabinose into the arabinan of arabinogalactan. May function as inverting enzyme using UDP-beta-L-arabinopyranoside. May be important for arabinan side chains of rhamnogalacturonan I (RG-I), a major component of pectins. Cell wall pectic arabinans are involved in thigmomorphogenesis response of inflorescence stems to mechanical stress. KEYWORDS: Cell wall biogenesis/degradation;Glycoprotein;Glycosyltransferase;Golgi apparatus;Membrane;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus membrane ; Single-pass type II membrane protein " Q6DBH0,PROTEIN NAMES: E3 ubiquitin-protein ligase APD2 (Protein ABERRANT POLLEN DEVELOPMENT 2) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Exhibits E2-dependent E3 ligase activity. Involved in pollen mitosis II (PMII) regulation during male gametogenesis. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Membrane;Metal-binding;Reference proteome;Transferase;Transmembrane;Transmembrane helix;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Endomembrane system ; Multi-pass membrane protein Note=Associated with intracellular membranes and in the tonoplast and endosomes in the germinating pollen tubes. Q6DGB6,"PROTEIN NAMES: Coiled-coil domain-containing protein 103 (Protein schmalhans) PROTEIN FAMILY: CCDC103/PR46b family ORGANISM: Danio rerio (Zebrafish) (Brachydanio rerio) SIMILARITY: Belongs to the CCDC103/PR46b family. FUNCTION: Dynein-attachment factor required for cilia motility. KEYWORDS: Cell projection;Cilium;Cilium biogenesis/degradation;Coiled coil;Cytoplasm;Flagellum;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Cell projection, cilium, flagellum " Q6DKI7,"PROTEIN NAMES: Transmembrane protein PVRIG (CD112 receptor) (CD112R) (Poliovirus receptor-related immunoglobulin domain-containing protein) ORGANISM: Homo sapiens (Human) FUNCTION: Cell surface receptor for NECTIN2. May act as a coinhibitory receptor that suppresses T-cell receptor-mediated signals. Following interaction with NECTIN2, inhibits T-cell proliferation. Competes with CD226 for NECTIN2-binding. KEYWORDS: Cell membrane;Membrane;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein MISCELLANEOUS: Was named PVRIG for the homology observed between its second exon and the variable immunoglobulin domain of the polio virus receptor (PVR/CD155) and polio virus receptor-like (PVRL) genes." Q6DN03,"PROTEIN NAMES: Putative histone H2B type 2-C (H2B-clustered histone 20 pseudogene) (Histone H2B.t) (H2B/t) PROTEIN FAMILY: Histone H2B family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the histone H2B family. FUNCTION: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. KEYWORDS: Acetylation;Chromosome;DNA-binding;Hydroxylation;Isopeptide bond;Methylation;Nucleosome core;Nucleus;Phosphoprotein;Reference proteome;Ubl conjugation SUBCELLULAR LOCATION: Nucleus. Chromosome. MISCELLANEOUS: The mouse orthologous protein seems not to exist." Q6DRD3,"PROTEIN NAMES: DNA polymerase beta (5'-deoxyribose-phosphate lyase) (5'-dRP lyase) (AP lyase) PROTEIN FAMILY: DNA polymerase type-X family ORGANISM: Danio rerio (Zebrafish) (Brachydanio rerio) SIMILARITY: Belongs to the DNA polymerase type-X family. FUNCTION: Repair polymerase that plays a key role in base-excision repair. During this process, the damaged base is excised by specific DNA glycosylases, the DNA backbone is nicked at the abasic site by an apurinic/apyrimidic (AP) endonuclease, and POLB removes 5'-deoxyribose-phosphate from the preincised AP site acting as a 5'-deoxyribose-phosphate lyase (5'-dRP lyase); through its DNA polymerase activity, it adds one nucleotide to the 3' end of the arising single-nucleotide gap. Conducts 'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. It is also able to cleave sugar-phosphate bonds 3' to an intact AP site, acting as an AP lyase. KEYWORDS: Cytoplasm;DNA damage;DNA repair;DNA replication;DNA synthesis;DNA-binding;DNA-directed DNA polymerase;Lyase;Magnesium;Metal-binding;Methylation;Nucleotidyltransferase;Nucleus;Potassium;Reference proteome;Sodium;Transferase;Ubl conjugation SUBCELLULAR LOCATION: Nucleus Cytoplasm Note=Cytoplasmic in normal conditions. Translocates to the nucleus following DNA damage. " Q6DVA0,"PROTEIN NAMES: LEM domain-containing protein 2 (Nuclear envelope transmembrane protein 25) (NET25) ORGANISM: Mus musculus (Mouse) FUNCTION: Nuclear lamina-associated inner nuclear membrane protein that is involved in nuclear structure organization and maintenance of nuclear envelope (NE) integrity and NE reformation after mitosis. Plays a role as transmembrane adapter for the endosomal sorting complexes required for transport (ESCRT), and is thereby involved in ESCRT-mediated NE reformation (By similarity). Promotes ESCRT-mediated NE closure by recruiting CHMP7 and downstream ESCRT-III proteins IST1/CHMP8 and CHMP2A to the reforming NE during anaphase (By similarity). During nuclear reassembly, condenses into a liquid-like coating around microtubule spindles and coassembles with CHMP7 to form a macromolecular O-ring seal at the confluence between membranes, chromatin, and the spindle to facilitate early nuclear sealing (By similarity). Plays a role in the organization of heterochromatin associated with the NE and in the maintenance of NE organization under mechanical stress. Required for embryonic development and is involved in regulation of several signaling pathways such as MAPK and AKT. Required for myoblast differentiation involving regulation of ERK signaling. Essential for cardiac homeostasis and proper heart function. KEYWORDS: Acetylation;Cytoplasm;Cytoskeleton;Membrane;Nucleus;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Nucleus inner membrane ; Multi-pass membrane protein Nucleus envelope Cytoplasm, cytoskeleton, spindle Note=Lamina-associated protein residing in the inner nuclear membrane (INM) of the nuclear envelope (NE). The localization to the INM is dependent on LMNA (By similarity). Evenly distributed around the NE during interphase. During metaphase, found in a reticular network (By similarity). Recruited to the reforming NE on chromatin disks in early anaphase (By similarity). In late anaphase, concentrates at the NE core proximal to spindle microtubules, and then broadening to a distributed nuclear rim pattern (By similarity). " Q6E1M8,"PROTEIN NAMES: Acyl-CoA wax alcohol acyltransferase 2 (11-cis-specific retinyl-ester synthase) (11-cis-RE-synthase) (Acyl-CoA retinol O-fatty-acyltransferase) (ARAT) (Retinol O-fatty-acyltransferase) (Diacylglycerol O-acyltransferase 2-like protein 4) (Long-chain-alcohol O-fatty-acyltransferase 2) (Wax synthase) (mWS) PROTEIN FAMILY: Diacylglycerol acyltransferase family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the diacylglycerol acyltransferase family. FUNCTION: Acyltransferase that catalyzes the formation of ester bonds between fatty alcohols and fatty acyl-CoAs to form wax monoesters. Shows a preference for medium chain acyl-CoAs from C12 to C16 in length and fatty alcohols shorter than C20, as the acyl donor and acceptor, respectively. Also possesses fatty acyl-CoA retinol acyltransferase (ARAT) activity that preferentially esterifies 11-cis-retinol, a chromophore precursor of bleached opsin pigments in cone cells. Shows higher catalytic efficiency toward 11-cis-retinol versus 9-cis-retinol, 13- cis-retinol and all-trans-retinol substrates. KEYWORDS: Acyltransferase;Alternative splicing;Endoplasmic reticulum;Lipid biosynthesis;Lipid metabolism;Membrane;Reference proteome;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein " Q6E593,"PROTEIN NAMES: Benzyl alcohol O-benzoyltransferase (PhBEBT1) (Acetyl CoA:3-hydroxybenzyl alcohol acetyltransferase) (Acetyl CoA:benzyl alcohol acetyltransferase) (Acetyl CoA:geraniol acetyltransferase) (Benzoyl coenzyme A:benzyl alcohol benzoyl transferase) (Benzyl CoA:benzyl alcohol/phenylethanol benzoyltransferase) (PhBPBT) (Benzyl CoA:(3Z)-hex-3-en-1-ol benzoyltransferase) (Benzyl CoA:2-phenylethanol benzoyltransferase) (Benzyl CoA:3-hydroxybenzyl alcohol benzoyltransferase) (Benzyl CoA:butanol benzoyltransferase) (Benzyl CoA:geraniol benzoyltransferase) (Benzyl CoA:octan-1-ol benzoyltransferase) PROTEIN FAMILY: Plant acyltransferase family ORGANISM: Petunia hybrida (Petunia) SIMILARITY: Belongs to the plant acyltransferase family. FUNCTION: Involved in the production of volatile organic compounds (VOCs), including floral volatile benzenoids and phenylpropanoids (FVBP), in flowers of fragrant cultivars (e.g. cv. Mitchell and cv. V26), scent attracting pollinators (e.g. the night-active hawkmoth pollinator Manduca sexta). Acyltransferase that catalyzes the transfer of benzoyl and acetyl moieties to a large variety of potential substrate alcohols, and involved in the formation of volatile esters benzyl benzoate and phenylethyl benzoate from benzoyl-CoA. With acetyl-CoA, mainly active on benzyl alcohol, and, to a lower extent, on 3-hydroxybenzyl alcohol, geraniol, and 2-phenylethanol, but barely active on butanol, 1-octanol, 4-hydroxy-benzyl alcohol, 2-hexanol, cis-3-hexen-1-ol and linalool. With benzoyl-CoA, mainly active on benzyl alcohol, but also efficient on several substrates, including 3-hydroxybenzyl alcohol, 2-phenylethanol, geraniol, butanol, cis-3-hexen-1-ol and 1-octanol. PATHWAY: Aromatic compound metabolism; benzoyl-CoA degradation. KEYWORDS: Acyltransferase;Transferase " Q6EPW7,PROTEIN NAMES: Cysteine and histidine-rich domain-containing protein RAR1 (CHORD domain-containing protein RAR1) (OsRAR1) (Protein REQUIRED FOR MLA12 RESISTANCE 1) ORGANISM: Oryza sativa subsp. japonica (Rice) FUNCTION: Involved in basal disease resistance to virulent strain of bacterial blight (X.oryzae) and compatible race of rice blast fungus (M.grisea). May act as positive regulator of basal defense. Associates with HSP90 and is essential for the pathogen-associated molecular pattern (PAMP)-triggered immune responses specifically enhanced by RAC1. KEYWORDS: Cytoplasm;Metal-binding;Nucleus;Plant defense;Reference proteome;Repeat;Zinc SUBCELLULAR LOCATION: Cytoplasm Nucleus Q6EV70,"PROTEIN NAMES: GDP-fucose protein O-fucosyltransferase 1 (Peptide-O-fucosyltransferase 1) (O-FucT-1) PROTEIN FAMILY: Glycosyltransferase 65 family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the glycosyltransferase 65 family. FUNCTION: Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue found in the consensus sequence C2-X(4,5)-[S/T]-C3 of EGF domains, where C2 and C3 are the second and third conserved cysteines. Specifically uses GDP-fucose as donor substrate and proper disulfide pairing of the substrate EGF domains is required for fucose transfer. Fucosylates AGRN and determines its ability to cluster acetylcholine receptors (AChRs) (By similarity). Plays a crucial role in NOTCH signaling. Initial fucosylation of NOTCH by POFUT1 generates a substrate for FRINGE/RFNG, an acetylglucosaminyltransferase that can then extend the fucosylation on the NOTCH EGF repeats. This extended fucosylation is required for optimal ligand binding and canonical NOTCH signaling induced by DLL1 or JAGGED1. PATHWAY: Protein modification; protein glycosylation. KEYWORDS: Carbohydrate metabolism;Disulfide bond;Endoplasmic reticulum;Fucose metabolism;Glycoprotein;Glycosyltransferase;Manganese;Notch signaling pathway;Reference proteome;Signal;Transferase SUBCELLULAR LOCATION: Endoplasmic reticulum " Q6EZC2,"PROTEIN NAMES: Subtilase cytotoxin subunit A PROTEIN FAMILY: Peptidase S8 family ORGANISM: Escherichia coli SIMILARITY: Belongs to the peptidase S8 family. FUNCTION: Protease subunit of subtilase cytotoxin SubAB5. An endoprotease specific for host endoplasmic reticulum (ER) chaperone BiP/HSPA5, has no activity on human HSP70 or HSPA8. Cleaves between 'Leu-416' and 'Leu-417' of BiP/HSPA5 in the hinge between BiP's ATPase and protein-binding domains. This induces host ER stress response and eventual cell death. Culture supernatant of E.coli expressing both subA and subB are toxic for Vero cells (African green monkey kidney cell line), Chinese hamster ovary cells and Hct-8 cells (human colonic epithelial cell line); the subunits are not toxic individually. Purified SubAB5 is highly toxic, <0.1 pg is able to kill at least 50% of 30'000 Vero cells in a microtiter plate assay after 3 days; no cytotoxicity is seen at 24 hours. Preabsorption with cells expressing a ganglioside GM2 mimic reduced cytotoxicity of SubAB5 by 93% in the Vero cytotoxicity assay. Intraperitoneal injection of 200 ng of purified SubAB5 kills mice; the higher the dose the faster the mice die. Animals injected intraperitoneally with purified SubAB5 have microvascular thrombi in the brain and other organs, including the renal tubules and glomeruli. Injection induces an unfolded response in mice. Mice fed E.coli cells expressing cloned SubAB5 experience drastic weight loss and appear ill and lethargic. Protein synthesis in Vero cells is transiently inhibited by SubAB5; both subunits are required for this effect. Inhibition of protein synthesis is prevented by brefeldin A; cells are arrested in the G1 phase. SubAB5 at 100 ng/ml induced caspase-dependent apoptosis in Vero cells through mitochondrial membrane damage. KEYWORDS: 3D-structure;Direct protein sequencing;Disulfide bond;Host cytoplasm;Host endoplasmic reticulum;Hydrolase;Plasmid;Protease;Secreted;Serine protease;Signal;Toxin;Virulence SUBCELLULAR LOCATION: Secreted Host cytoplasm, host cytosol Host endoplasmic reticulum lumen Note=Colocalizes with host BiP/HSPA5 in the endoplasmic reticulum of Vero cells, its activity on BiP/HSPA5 is blocked by pretreatment with brefeldin A, which disrupts the Golgi apparatus and inhibits retrograde transport from the cell surface to the Golgi. Using different inhibitors it has been shown to be actively internalized by membrane-bound vesicles and undergoes clathrin-dependent retrograde transport, via early endosomes and the Golgi network, to the endoplasmic reticulum. Trafficking is similar in Vero cells, human HeLa cells and murine N2A cells. MISCELLANEOUS: The E.coli strain this operon was isolated from causes hemolytic uremic syndrome (HUS) and also encodes Stx, a Shigella-type toxin." Q6F3I7,"PROTEIN NAMES: Dipeptidyl aminopeptidase 4 (Dipeptidyl aminopeptidase IV) (DAP IV) PROTEIN FAMILY: Peptidase S9B family ORGANISM: Pseudoxanthomonas mexicana SIMILARITY: Belongs to the peptidase S9B family. FUNCTION: Catalyzes the sequential release of Tyr-Pro, Phe-Pro and Gly-Pro from the N-terminus of peptides and proteins. Is able to cleaves bioactive peptide beta-casomorphin. KEYWORDS: 3D-structure;Alternative initiation;Aminopeptidase;Cytoplasm;Direct protein sequencing;Hydrolase;Periplasm;Protease;Signal SUBCELLULAR LOCATION: [Isoform 2]: Cytoplasm.; SUBCELLULAR LOCATION: [Isoform 1]: Periplasm " Q6F4F5,"PROTEIN NAMES: Cytochrome P450 724B1 (OsCYP724B1) ((22S)-22-hydroxycampesterol synthase) (Dwarf protein 11) (OsDWARF11) PROTEIN FAMILY: Cytochrome P450 family ORGANISM: Oryza sativa subsp. japonica (Rice) SIMILARITY: Belongs to the cytochrome P450 family. FUNCTION: Involved in brassinosteroid biosynthesis. May catalyze a C6-oxidation step and may be involved to supply 6-deoxotyphasterol and typhasterol. Involved in internode elongation and seed development. Catalyzes the conversion of campesterol (CR) to (22S)-22-hydroxycampesterol (22-OHCR, 22-hydroxyCR). PATHWAY: Plant hormone biosynthesis; brassinosteroid biosynthesis. KEYWORDS: Brassinosteroid biosynthesis;Heme;Iron;Lipid biosynthesis;Lipid metabolism;Membrane;Metal-binding;Monooxygenase;Oxidoreductase;Reference proteome;Steroid biosynthesis;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Single-pass membrane protein " Q6F4M8,"PROTEIN NAMES: 4-nitrophenol 4-monooxygenase/4-nitrocatechol 2-monooxygenase, oxygenase component (4-NP/4-NCA monooxygenase) (PNP monooxygenase) PROTEIN FAMILY: FADH(2)-utilizing monooxygenase family ORGANISM: Rhodococcus opacus (Nocardia opaca) SIMILARITY: Belongs to the FADH(2)-utilizing monooxygenase family. FUNCTION: Involved in the degradation of para-nitrophenol (4-NP). Catalyzes both the initial hydroxylation of 4-NP to produce 4-nitrocatechol (4-NCA) and the subsequent oxidative release of the nitro group from 4-NCA to produce 2-hydroxy-1,4-benzoquinone. It can also use 4-nitroresorcinol as substrate with a rate of nitrite release similar to that observed with the two physiological substrates, 4-PN and 4-NCA. PATHWAY: Aromatic compound metabolism.; PATHWAY: Xenobiotic degradation. KEYWORDS: Aromatic hydrocarbons catabolism;FAD;Flavoprotein;Monooxygenase;NAD;Oxidoreductase " Q6FL58,"PROTEIN NAMES: Serine/threonine-protein kinase ATG1 (Autophagy-related protein 1) PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family, APG1/unc-51/ULK1 subfamily ORGANISM: Candida glabrata (strain ATCC 2001 / BCRC 20586 / JCM 3761 / NBRC 0622 / NRRL Y-65 / CBS 138) (Yeast) (Nakaseomyces glabratus) SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily. FUNCTION: Serine/threonine protein kinase involved in the cytoplasm to vacuole transport (Cvt) and found to be essential in autophagy, where it is required for the formation of autophagosomes. Involved in the clearance of protein aggregates which cannot be efficiently cleared by the proteasome (By similarity). Required for selective autophagic degradation of the nucleus (nucleophagy) as well as for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production (By similarity). Also involved in endoplasmic reticulum-specific autophagic process, in selective removal of ER-associated degradation (ERAD) substrates (By similarity). Plays a key role in ATG9 and ATG23 cycling through the pre-autophagosomal structure and is necessary to promote ATG18 binding to ATG9 through phosphorylation of ATG9 (By similarity). Catalyzes phosphorylation of ATG4, decreasing the interaction between ATG4 and ATG8 and impairing deconjugation of PE-conjugated forms of ATG8 (By similarity). Contributes to virulence by conferring resistance to unstable nutrient environments and immune defense of hosts. KEYWORDS: ATP-binding;Autophagy;Cytoplasm;Kinase;Membrane;Nucleotide-binding;Protein transport;Reference proteome;Serine/threonine-protein kinase;Transferase;Transport SUBCELLULAR LOCATION: Cytoplasm Preautophagosomal structure membrane ; Peripheral membrane protein " Q6GPL3,"PROTEIN NAMES: Aurora kinase B-B (Aurora/IPL1-related kinase 2-B) (AIRK2-B) (XAIRK2-B) (Serine/threonine-protein kinase 12-B) (Serine/threonine-protein kinase aurora-B-B) (xAurora-B-B) PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family, Aurora subfamily ORGANISM: Xenopus laevis (African clawed frog) SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily. FUNCTION: Serine/threonine-protein kinase component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Involved in the bipolar attachment of spindle microtubules to kinetochores and is a key regulator for the onset of cytokinesis during mitosis. Required for central/midzone spindle assembly and cleavage furrow formation. Key component of the cytokinesis checkpoint, a process required to delay abscission to prevent both premature resolution of intercellular chromosome bridges and accumulation of DNA damage. Phosphorylates 'Ser-10' of histone H3 during mitosis. KEYWORDS: ATP-binding;Cell cycle;Cell division;Centromere;Chromosome;Chromosome partition;Cytoplasm;Cytoskeleton;Kinase;Mitosis;Nucleotide-binding;Nucleus;Phosphoprotein;Reference proteome;Serine/threonine-protein kinase;Transferase SUBCELLULAR LOCATION: Nucleus Chromosome Chromosome, centromere Cytoplasm, cytoskeleton, spindle Midbody Note=Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Localization (and probably targeting of the CPC) to the inner centromere occurs predominantly in regions with overlapping mitosis-specific histone phosphorylations H3pT3 and H2ApT12. " Q6GTX8,"PROTEIN NAMES: Leukocyte-associated immunoglobulin-like receptor 1 (LAIR-1) (hLAIR1) (CD antigen CD305) ORGANISM: Homo sapiens (Human) FUNCTION: Functions as an inhibitory receptor that plays a constitutive negative regulatory role on cytolytic function of natural killer (NK) cells, B-cells and T-cells. Activation by Tyr phosphorylation results in recruitment and activation of the phosphatases PTPN6 and PTPN11. It also reduces the increase of intracellular calcium evoked by B-cell receptor ligation. May also play its inhibitory role independently of SH2-containing phosphatases. Modulates cytokine production in CD4+ T-cells, down-regulating IL2 and IFNG production while inducing secretion of transforming growth factor beta. Down-regulates also IgG and IgE production in B-cells as well as IL8, IL10 and TNF secretion. Inhibits proliferation and induces apoptosis in myeloid leukemia cell lines as well as prevents nuclear translocation of NF-kappa-B p65 subunit/RELA and phosphorylation of I-kappa-B alpha/CHUK in these cells. Inhibits the differentiation of peripheral blood precursors towards dendritic cells. KEYWORDS: 3D-structure;Adaptive immunity;Alternative splicing;Cell membrane;Disulfide bond;Glycoprotein;Immunity;Immunoglobulin domain;Membrane;Phosphoprotein;Receptor;Reference proteome;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein MISCELLANEOUS: [Isoform 2]: Functions as an inhibitory receptor in NK cells and T-cells." Q6GZ04,"PROTEIN NAMES: Phenylalanine aminomutase (L-beta-phenylalanine forming) (Phenylalanine ammonia-lyase) PROTEIN FAMILY: PAL/histidase family ORGANISM: Taxus canadensis (Canadian yew) SIMILARITY: Belongs to the PAL/histidase family. FUNCTION: Phenylalanine aminomutase that catalyzes the rearrangement of L-phenylalanine to R-beta-phenylalanine. Catalyzes the first committed step in the biosynthesis of the side chain of the alkaloid taxol (paclitaxel), a widely-used compound with antitumor activity. Also has low phenylalanine ammonia-lyase activity. PATHWAY: Alkaloid biosynthesis; taxol biosynthesis.; PATHWAY: Phenylpropanoid metabolism; trans-cinnamate biosynthesis; trans-cinnamate from L-phenylalanine: step 1/1. KEYWORDS: 3D-structure;Alkaloid metabolism;Cytoplasm;Isomerase;Lyase;Phenylalanine catabolism;Phenylpropanoid metabolism;Taxol biosynthesis SUBCELLULAR LOCATION: Cytoplasm " Q6H1V1,"PROTEIN NAMES: Bestrophin-3 (Vitelliform macular dystrophy 2-like protein 3) PROTEIN FAMILY: Anion channel-forming bestrophin family, Calcium-sensitive chloride channel subfamily ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the anion channel-forming bestrophin (TC 1.A.46) family. Calcium-sensitive chloride channel subfamily. FUNCTION: [Isoform 1]: Ligand-gated anion channel that allows the movement of chloride monoatomic anions across cell membranes when activated by calcium (Ca2+).; FUNCTION: [Isoform 2]: Does not function as calcium-gated chloride channel. KEYWORDS: Alternative splicing;Calcium;Cell membrane;Chloride;Chloride channel;Ion channel;Ion transport;Membrane;Metal-binding;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: [Isoform 1]: Cell membrane Note=Localized also in punctate structures in the cytosolic compartment.; SUBCELLULAR LOCATION: [Isoform 2]: Cell membrane Note=Localized also in punctate structures in the cytosolic compartment. " Q6H501,"PROTEIN NAMES: Cation transporter HKT1;3 (OsHKT1;3) (Cation transporter HKT6) (OsHKT6) PROTEIN FAMILY: TrkH potassium transport family, HKT subfamily ORGANISM: Oryza sativa subsp. japonica (Rice) SIMILARITY: Belongs to the TrkH potassium transport family. HKT (TC 2.A.38.3) subfamily. FUNCTION: Functions as a highly-selective sodium transporter. Does not seem to function as sodium-potassium cotransporter. May be involved in turgor changes for rolling and unrolling of leaves in response to environmental variations. KEYWORDS: Endoplasmic reticulum;Golgi apparatus;Ion transport;Membrane;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein Golgi apparatus membrane ; Multi-pass membrane protein " Q6H9K0,"PROTEIN NAMES: Exopolygalacturonase (Pollen allergen Pla a 2) (allergen Pla a 2.0101) PROTEIN FAMILY: Glycosyl hydrolase 28 family ORGANISM: Platanus acerifolia (London plane tree) SIMILARITY: Belongs to the glycosyl hydrolase 28 family. FUNCTION: May function in depolymerizing pectin during pollen development, germination, and tube growth. Acts as an exo-polygalacturonase. KEYWORDS: Allergen;Cell wall;Cell wall biogenesis/degradation;Direct protein sequencing;Endoplasmic reticulum;Glycoprotein;Glycosidase;Golgi apparatus;Hydrolase;Repeat;Secreted;Signal SUBCELLULAR LOCATION: Secreted Secreted, cell wall Golgi apparatus Endoplasmic reticulum Vesicle Note=After 5 minutes in germination medium, detected as a soluble protein and released from apertural and non-apertural regions of pollen grain wall. After 15 and 30 minutes, abundant in the germinal aperture. After 2 hours in germination medium, localization in these regions decreases. In mature, non-hydrated pollen grains, abundant in cisternae and vesicles of Golgi in the apertural region. Sparse in the center of the cytoplasm, associated with cisternae and vesicles of the endoplasmic reticulum (ER) and abundant in the generative cell wall. After 5 minutes of hydration, localization to these regions decreases. After 15 minutes of hydration, present around emergent pollen tube and in the center of the vegetative cell, both in the ER and Golgi. After 30 minutes of hydration, present in juncture between pollen wall and pollen tube. Abundant in the ER cisternae, which show a disorganized and interrupted concentric structure with numerous ER vesicles linked to Golgi vesicles. " Q6HA09,"PROTEIN NAMES: Astacin-like metalloendopeptidase (Oocyte astacin) (Ovastacin) (Sperm acrosomal SLLP1-binding protein) ORGANISM: Mus musculus (Mouse) FUNCTION: Oocyte-specific oolemmal receptor involved in sperm and egg adhesion and fertilization. Plays a role in the polyspermy inhibition. Probably acts as a protease for the post-fertilization cleavage of ZP2. Cleaves the sperm-binding ZP2 at the surface of the zona pellucida after fertilization and cortical granule exocytosis, rendering the zona pellucida unable to support further sperm binding. KEYWORDS: Alternative splicing;Cell membrane;Cytoplasm;Cytoplasmic vesicle;Disulfide bond;Fertilization;Hydrolase;Membrane;Metal-binding;Metalloprotease;Protease;Reference proteome;Signal;Zinc;Zymogen SUBCELLULAR LOCATION: Cytoplasm Cell membrane Cytoplasmic vesicle, secretory vesicle, Cortical granule Note=Probably exocytosed from cortical granules during post-fertilization. Detected throughout the ooplasm of germinal vesicle stage oocytes in early bilaminar secondary follicles at postnatal (PN) day 3. Detected in the microvillar domain of the oolemma in arrested ovulated secondary oocytes and in the first polar body prior to fertilization. Upon fertilization, detected in the perivitelline space (PVS) and occasionally on the oolemma in 2-cell through morulae stages. Colocalizes with SPACA3 at the microvillar domain of the oolemma and in the perivitelline space (PVS). " Q6IEG0,PROTEIN NAMES: U11/U12 small nuclear ribonucleoprotein 48 kDa protein (U11/U12 snRNP 48 kDa protein) (U11/U12-48K) ORGANISM: Homo sapiens (Human) FUNCTION: Likely involved in U12-type 5' splice site recognition. KEYWORDS: 3D-structure;Alternative splicing;Isopeptide bond;Metal-binding;mRNA processing;mRNA splicing;Nucleus;Reference proteome;Spliceosome;Ubl conjugation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Q6IMP4,PROTEIN NAMES: Pannexin-2 PROTEIN FAMILY: Pannexin family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the pannexin family. FUNCTION: Ion channel with a slight anion preference. Also able to release ATP (By similarity). Plays a role in regulating neurogenesis and apoptosis in keratinocytes. KEYWORDS: Alternative splicing;Cell membrane;Endoplasmic reticulum;Glycoprotein;Golgi apparatus;Ion channel;Ion transport;Membrane;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Golgi apparatus membrane ; Multi-pass membrane protein Endoplasmic reticulum membrane ; Multi-pass membrane protein Note=Localizes to Golgi apparatus and endoplasmic reticulum in multipotential neural stem and progenitor cells and to plasma membrane in terminally differentiated neurons. Q6IN84,"PROTEIN NAMES: rRNA methyltransferase 1, mitochondrial (16S rRNA (guanosine(1145)-2'-O)-methyltransferase) (16S rRNA [Gm1145] 2'-O-methyltransferase) PROTEIN FAMILY: Class IV-like SAM-binding methyltransferase superfamily, RNA methyltransferase TrmH family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family. FUNCTION: S-adenosyl-L-methionine-dependent 2'-O-ribose methyltransferase that catalyzes the formation of 2'-O-methylguanosine at position 1145 (Gm1145) in the 16S mitochondrial large subunit ribosomal RNA (mtLSU rRNA), a universally conserved modification in the peptidyl transferase domain of the mtLSU rRNA. KEYWORDS: Alternative splicing;Methyltransferase;Mitochondrion;Reference proteome;rRNA processing;S-adenosyl-L-methionine;Transferase;Transit peptide SUBCELLULAR LOCATION: Mitochondrion matrix " Q6IP18,"PROTEIN NAMES: Replication protein A 32 kDa subunit-A (RP-A p32) (Replication factor A protein 2) (RF-A protein 2) (Replication protein A 34 kDa subunit) (RP-A p34) PROTEIN FAMILY: Replication factor A protein 2 family ORGANISM: Xenopus laevis (African clawed frog) SIMILARITY: Belongs to the replication factor A protein 2 family. FUNCTION: As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. KEYWORDS: DNA damage;DNA recombination;DNA repair;DNA replication;DNA-binding;Nucleus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Nucleus Nucleus, PML body Note=Redistributes to discrete nuclear foci upon DNA damage in an ATR-dependent manner. " Q6IPU0,"PROTEIN NAMES: Centromere protein P (CENP-P) PROTEIN FAMILY: CENP-P/CTF19 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the CENP-P/CTF19 family. FUNCTION: Component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. May be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex. KEYWORDS: 3D-structure;Alternative splicing;Centromere;Chromosome;Coiled coil;Nucleus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Nucleus Chromosome, centromere Note=Localizes exclusively in the centromeres. The CENPA-CAD complex is probably recruited on centromeres by the CENPA-NAC complex. " Q6IQX8,"PROTEIN NAMES: Zinc finger protein 219 PROTEIN FAMILY: Krueppel C2H2-type zinc-finger protein family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein family. FUNCTION: Transcriptional regulator. Recognizes and binds 2 copies of the core DNA sequence motif 5'-GGGGG-3'. Binds to the HMGN1 promoter and may repress HMGN1 expression (By similarity). Regulates SNCA expression in primary cortical neurons (By similarity). Binds to the COL2A1 promoter and activates COL2A1 expression, as part of a complex with SOX9. Plays a role in chondrocyte differentiation. KEYWORDS: Activator;Differentiation;DNA-binding;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Repeat;Repressor;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus " Q6IS41,"PROTEIN NAMES: Solute carrier family 25 member 47 (Hepatocellular carcinoma down-regulated mitochondrial carrier homolog) (Mitochondrial NAD(+) transporter SLC25A47) PROTEIN FAMILY: Mitochondrial carrier family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the mitochondrial carrier (TC 2.A.29) family. FUNCTION: Mitochondrial NAD(+) transporter that acts as a 'metabolic gate' in hepatic lipogenesis. Provides NAD(+) substrate to mitochondrial SIRT3 deacetylase and enables its NAD(+)-dependent activities in mitochondrial energy metabolism. This triggers downstream activation of PRKAA1/AMPK-alpha signaling cascade that negatively regulates sterol regulatory element-binding protein (SREBP) transcriptional activities and ATP-consuming lipogenesis to restore cellular energy balance. May transport other mitochondrial metabolites having an aromatic nucleotide and phosphate groups, such as acetyl-CoA. Does not transport amino acids. The transport mechanism remains to be elucidated. KEYWORDS: Membrane;Mitochondrion;Mitochondrion inner membrane;Mitochondrion outer membrane;Reference proteome;Repeat;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Mitochondrion inner membrane ; Multi-pass membrane protein Mitochondrion outer membrane ; Multi-pass membrane protein MISCELLANEOUS: SLC25A47 is transcriptionally up-regulated in response to antihyperglycemic drug metformin. Promotes metformin pharmacological effects." Q6ISB3,"PROTEIN NAMES: Grainyhead-like protein 2 homolog (Brother of mammalian grainyhead) (Transcription factor CP2-like 3) PROTEIN FAMILY: Grh/CP2 family, Grainyhead subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the grh/CP2 family. Grainyhead subfamily. FUNCTION: Transcription factor playing an important role in primary neurulation and in epithelial development. Binds directly to the consensus DNA sequence 5'-AACCGGTT-3' acting as an activator and repressor on distinct target genes (By similarity). During embryogenesis, plays unique and cooperative roles with GRHL3 in establishing distinct zones of primary neurulation. Essential for closure 3 (rostral end of the forebrain), functions cooperatively with GRHL3 in closure 2 (forebrain/midbrain boundary) and posterior neuropore closure (By similarity). Regulates epithelial morphogenesis acting as a target gene-associated transcriptional activator of apical junctional complex components. Up-regulates of CLDN3 and CLDN4, as well as of RAB25, which increases the CLDN4 protein and its localization at tight junctions (By similarity). Comprises an essential component of the transcriptional machinery that establishes appropriate expression levels of CLDN4 and CDH1 in different types of epithelia. Exhibits functional redundancy with GRHL3 in epidermal morphogenetic events and epidermal wound repair (By similarity). In lung, forms a regulatory loop with NKX2-1 that coordinates lung epithelial cell morphogenesis and differentiation (By similarity). In keratinocytes, plays a role in telomerase activation during cellular proliferation, regulates TERT expression by binding to TERT promoter region and inhibiting DNA methylation at the 5'-CpG island, possibly by interfering with DNMT1 enzyme activity. In addition, impairs keratinocyte differentiation and epidermal function by inhibiting the expression of genes clustered at the epidermal differentiation complex (EDC) as well as GRHL1 and GRHL3 through epigenetic mechanisms. KEYWORDS: 3D-structure;Activator;Alternative splicing;Corneal dystrophy;Deafness;Disease variant;DNA-binding;Dwarfism;Ectodermal dysplasia;Membrane;Non-syndromic deafness;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus Membrane Note=detected at cell-cell contact areas. MISCELLANEOUS: GRHL genes (GRHL1, GRHL2 and GRHL3) show a paradoxal lack of redundancy despite their extensive sequence identity in the DNA-binding and protein dimerization domains and the fact that the core consensus DNA binding sites are identical. They have related but remarkably different functions during embryogenesis because of their differential spatiotemporal expression patterns during development." Q6IUP1,"PROTEIN NAMES: Spermatogenesis- and oogenesis-specific basic helix-loop-helix-containing protein 1 ORGANISM: Mus musculus (Mouse) FUNCTION: Transcription regulator of both male and female germline differentiation. Suppresses genes involved in spermatogonial stem cells maintenance, and induces genes important for spermatogonial differentiation. Coordinates oocyte differentiation without affecting meiosis I. KEYWORDS: Cytoplasm;Developmental protein;Differentiation;DNA-binding;Nucleus;Reference proteome;Spermatogenesis;Transcription;Transcription regulation SUBCELLULAR LOCATION: Cytoplasm Nucleus " Q6IVY4,"PROTEIN NAMES: Protein phosphatase Slingshot homolog (xSSH) (Slingshot-related protein) PROTEIN FAMILY: Protein-tyrosine phosphatase family ORGANISM: Xenopus laevis (African clawed frog) SIMILARITY: Belongs to the protein-tyrosine phosphatase family. FUNCTION: Protein phosphatase which regulates actin filament dynamics. Dephosphorylates and activates the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and stimulates their disassembly. Required for completion of the gastrulation movement and for cytokinesis. KEYWORDS: Actin-binding;Alternative splicing;Coiled coil;Cytoplasm;Cytoskeleton;Hydrolase;Protein phosphatase;Reference proteome SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton Cleavage furrow Midbody Note=Also localizes to the cleavage furrow and the midbody during cytokinesis. MISCELLANEOUS: Tyrosine phosphatase activity has not been demonstrated for this protein to date." Q6IZB5,"PROTEIN NAMES: Solute carrier family 25 member 32 (Mitochondrial FAD transporter) PROTEIN FAMILY: Mitochondrial carrier family ORGANISM: Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) SIMILARITY: Belongs to the mitochondrial carrier (TC 2.A.29) family. FUNCTION: Facilitates flavin adenine dinucleotide (FAD) translocation across the mitochondrial inner membrane into the mitochondrial matrix where it acts as a redox cofactor to assist flavoenzyme activities in fundamental metabolic processes including fatty acid beta-oxidation, amino acid and choline metabolism as well as mitochondrial electron transportation. In particular, provides FAD to DLD dehydrogenase of the glycine cleavage system, part of mitochondrial one-carbon metabolic pathway involved in neural tube closure in early embryogenesis. KEYWORDS: Membrane;Mitochondrion;Mitochondrion inner membrane;Repeat;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Mitochondrion inner membrane ; Multi-pass membrane protein " Q6J1Z6,"PROTEIN NAMES: 2-aminophenol 1,6-dioxygenase subunit beta (2-amino-5-chlorophenol 1,6-dioxygenase subunit beta) PROTEIN FAMILY: LigB/MhpB extradiol dioxygenase family ORGANISM: Comamonas testosteroni (Pseudomonas testosteroni) SIMILARITY: Belongs to the LigB/MhpB extradiol dioxygenase family. FUNCTION: Component of the 2-aminophenol 1,6-dioxygenase (APD) complex that catalyzes the ring fission of 2-aminophenol to produce 2-aminomuconic semialdehyde. CnbCb seems to be the catalytic subunit of the complex. Also active on other substrates such as 2-amino-5-chlorophenol (68% activity), protocatechuate (33% activity) and catechol (5% activity). Both 2-aminophenol and 2-amino-5-cholorophenol are likely native substrates for this dioxygenase which is involved in the reductive degradation pathway of both nitrobenzene (NB) and 4-chloronitrobenzene (4-CNB), allowing C.testosteroni strain CNB-1 to grow on these compounds as sole source of carbon, nitrogen, and energy. PATHWAY: Xenobiotic degradation; nitrobenzene degradation.; PATHWAY: Xenobiotic degradation; 4-chloronitrobenzene degradation. KEYWORDS: 3D-structure;Aromatic hydrocarbons catabolism;Dioxygenase;Direct protein sequencing;Iron;Metal-binding;Oxidoreductase;Plasmid MISCELLANEOUS: Not active on 4-methylcatechol, 4-chlorocatechol, 2,4-dihydroxybenzoate, o-nitrophenol, p-nitrophenol or 4-nitrocatechol." Q6JP77,"PROTEIN NAMES: A-kinase anchor protein 7 isoforms delta and gamma (AKAP-7 isoforms delta and gamma) (A-kinase anchor protein 18) (AKAP-18) (Protein kinase A-anchoring protein 7 isoforms delta and gamma) (PRKA7 isoforms delta and gamma) ORGANISM: Rattus norvegicus (Rat) FUNCTION: Probably targets cAMP-dependent protein kinase (PKA) to the cellular membrane or cytoskeletal structures. The membrane-associated form reduces epithelial sodium channel (ENaC) activity, whereas the free cytoplasmic form may negatively regulate ENaC channel feedback inhibition by intracellular sodium (By similarity). Isoform Delta may be involved in shuttling aquaporin-2 (AQP2) to the plasma membrane. KEYWORDS: 3D-structure;Alternative splicing;Cell membrane;Cytoplasm;Membrane;Nucleus;Reference proteome SUBCELLULAR LOCATION: [Isoform Gamma]: Nucleus Cytoplasm.; SUBCELLULAR LOCATION: [Isoform Delta]: Nucleus Cell membrane Note=Cotranslocates with AQP2 to the plasma membrane in response to arginine-vasopressin (AVP) stimulation in inner medullary collecting duct (IMCD) cells. " Q6L1T2,"PROTEIN NAMES: D-gluconate/D-galactonate dehydratase (GAD) (GNAD) PROTEIN FAMILY: Mandelate racemase/muconate lactonizing enzyme family, GaD subfamily ORGANISM: Picrophilus torridus (strain ATCC 700027 / DSM 9790 / JCM 10055 / NBRC 100828 / KAW 2/3) SIMILARITY: Belongs to the mandelate racemase/muconate lactonizing enzyme family. GaD subfamily. FUNCTION: Involved in the degradation of glucose and galactose via the nonphosphorylative variant of Entner-Doudoroff pathway. Catalyzes the dehydration of gluconate to produce 2-keto-3-deoxygluconate (KDG). It is also able to catalyze the dehydration of galactonate to produce 2-keto-3-deoxygalactonate (KDGal). PATHWAY: Carbohydrate acid metabolism; D-gluconate degradation. KEYWORDS: Carbohydrate metabolism;Lyase;Magnesium;Metal-binding " Q6L8H1,"PROTEIN NAMES: Keratin-associated protein 5-4 (Keratin-associated protein 5.4) (Ultrahigh sulfur keratin-associated protein 5.4) PROTEIN FAMILY: KRTAP type 5 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the KRTAP type 5 family. FUNCTION: In the hair cortex, hair keratin intermediate filaments are embedded in an interfilamentous matrix, consisting of hair keratin-associated protein (KRTAP), which are essential for the formation of a rigid and resistant hair shaft through their extensive disulfide bond cross-linking with abundant cysteine residues of hair keratins. The matrix proteins include the high-sulfur and high-glycine-tyrosine keratins. KEYWORDS: Keratin;Reference proteome;Repeat " Q6LZM9,PROTEIN NAMES: O-phosphoseryl-tRNA(Sec) selenium transferase (Selenocysteine synthase) (Sec synthase) (Selenocysteinyl-tRNA(Sec) synthase) (Sep-tRNA:Sec-tRNA synthase) (SepSecS) PROTEIN FAMILY: SepSecS family ORGANISM: Methanococcus maripaludis (strain DSM 14266 / JCM 13030 / NBRC 101832 / S2 / LL) SIMILARITY: Belongs to the SepSecS family. FUNCTION: Converts O-phosphoseryl-tRNA(Sec) to selenocysteinyl-tRNA(Sec) required for selenoprotein biosynthesis. PATHWAY: Aminoacyl-tRNA biosynthesis; selenocysteinyl-tRNA(Sec) biosynthesis; selenocysteinyl-tRNA(Sec) from L-seryl-tRNA(Sec) (archaeal/eukaryal route): step 2/2. KEYWORDS: 3D-structure;Protein biosynthesis;Pyridoxal phosphate;Reference proteome;RNA-binding;Selenium;Transferase;tRNA-binding Q6NKX5,"PROTEIN NAMES: Protection of telomeres protein 1b (AtPOT1b) (Protection of telomeres protein 2) (AtPot2) PROTEIN FAMILY: Telombin family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the telombin family. FUNCTION: Negatively regulates telomerase activity and participates in chromosome end protection. Binds RNA non-specifically. Associates with a regulatory Pol III-dependent lncRNA, which represses telomerase activity in response to DNA damage. Binds single-stranded telomeric DNA with weak affinity. KEYWORDS: Alternative splicing;Chromosome;DNA-binding;Nucleus;Reference proteome;RNA-binding;Telomere SUBCELLULAR LOCATION: Nucleus Chromosome, telomere " Q6NPN4,"PROTEIN NAMES: LysM domain-containing GPI-anchored protein 3 ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Required as a cell surface receptor for peptidoglycan (PGN) elicitor signaling leading to innate immunity. Plays an essential role in detecting PGNs and restricting bacterial growth (of Pseudomonas syringae pv. tomato DC3000 for example). KEYWORDS: Cell membrane;Disulfide bond;Glycoprotein;GPI-anchor;Lipoprotein;Membrane;Plant defense;Reference proteome;Repeat;Signal SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor, GPI-anchor. " Q6NPS8,"PROTEIN NAMES: NADPH-dependent diflavin oxidoreductase 1 (NADPH-dependent FMN and FAD-containing oxidoreductase) PROTEIN FAMILY: NADPH-dependent diflavin oxidoreductase NDOR1 family; Flavodoxin family; Flavoprotein pyridine nucleotide cytochrome reductase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the NADPH-dependent diflavin oxidoreductase NDOR1 family.; SIMILARITY: In the N-terminal section; belongs to the flavodoxin family.; SIMILARITY: In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. FUNCTION: NADPH-dependent reductase which is a central component of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery. Transfers electrons from NADPH via its FAD and FMN prosthetic groups to the [2Fe-2S] cluster of the anamorsin/DRE2 homolog, another key component of the CIA machinery. In turn, this reduced cluster provides electrons for assembly of cytosolic iron-sulfur cluster proteins (By similarity). Catalyzes the NADP-dependent reduction of cytochrome c, but not cytochrome P450 in vitro. Required for embryo development. KEYWORDS: Cytoplasm;FAD;Flavoprotein;FMN;NADP;Nucleus;Oxidoreductase;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Nucleus " Q6NSI4,"PROTEIN NAMES: RPA-related protein RADX (RPA-related and RAD51-antagonist, X-chromosome) ORGANISM: Homo sapiens (Human) FUNCTION: Single-stranded DNA-binding protein recruited to replication forks to maintain genome stability. Prevents fork collapse by antagonizing the accumulation of RAD51 at forks to ensure the proper balance of fork remodeling and protection without interfering with the capacity of cells to complete homologous recombination of double-strand breaks. KEYWORDS: 3D-structure;Alternative splicing;Chromosome;DNA-binding;Reference proteome SUBCELLULAR LOCATION: Chromosome Note=Recruited to replication forks. " Q6NUJ5,"PROTEIN NAMES: PWWP domain-containing protein 2B ORGANISM: Homo sapiens (Human) FUNCTION: Chromatin-binding protein that acts as an adapter between distinct nucleosome components (H3K36me3 or H2A.Z) and chromatin-modifying complexes, contributing to the regulation of the levels of histone acetylation at actively transcribed genes. Competes with CHD4 and MBD3 for interaction with MTA1 to form a NuRD subcomplex, preventing the formation of full NuRD complex (containing CHD4 and MBD3), leading to recruitment of HDACs to gene promoters resulting in turn in the deacetylation of nearby H3K27 and H2A.Z. Plays a role in facilitating transcriptional elongation through regulation of histone acetylation (By similarity). Negatively regulates brown adipocyte thermogenesis by interacting with and stabilizing HDAC1 at the UCP1 gene promoter, thereby promoting histone deacetylation at the promoter leading to the repression of UCP1 expression (By similarity). KEYWORDS: 3D-structure;Alternative splicing;Phosphoprotein;Reference proteome;Transcription;Transcription regulation " Q6NUQ1,"PROTEIN NAMES: RAD50-interacting protein 1 (RAD50 interactor 1) (HsRINT-1) (RINT-1) PROTEIN FAMILY: RINT1 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the RINT1 family. FUNCTION: Involved in regulation of membrane traffic between the Golgi and the endoplasmic reticulum (ER); the function is proposed to depend on its association in the NRZ complex which is believed to play a role in SNARE assembly at the ER. May play a role in cell cycle checkpoint control. Essential for telomere length control. KEYWORDS: Cell cycle;Coiled coil;Cytoplasm;Direct protein sequencing;Disease variant;Endoplasmic reticulum;ER-Golgi transport;Membrane;Protein transport;Reference proteome;Transport SUBCELLULAR LOCATION: Cytoplasm. Endoplasmic reticulum membrane; Peripheral membrane protein. MISCELLANEOUS: According to previous research, a longer form, which may be due to the differential initiation of translation using a non-AUG codon, may exist. However, the existence of such form has not been clearly demonstrated." Q6NWF1,"PROTEIN NAMES: Solute carrier family 2, facilitated glucose transporter member 12 (Glucose transporter type 12) (GLUT-12) PROTEIN FAMILY: Major facilitator superfamily, Sugar transporter family, Glucose transporter subfamily ORGANISM: Danio rerio (Zebrafish) (Brachydanio rerio) SIMILARITY: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily. FUNCTION: Insulin-regulated facilitative glucose transporter. KEYWORDS: Cell membrane;Cytoplasm;Glycoprotein;Membrane;Reference proteome;Sugar transport;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Endomembrane system ; Multi-pass membrane protein Cytoplasm, perinuclear region Note=Localizes primarily perinuclear region in the absence of insulin. " Q6NXW6,"PROTEIN NAMES: Cell cycle checkpoint protein RAD17 PROTEIN FAMILY: Rad17/RAD24 family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the rad17/RAD24 family. FUNCTION: Essential for sustained cell growth, maintenance of chromosomal stability, and ATR-dependent checkpoint activation upon DNA damage (By similarity). Has a weak ATPase activity required for binding to chromatin (By similarity). Participates in the recruitment of the 9-1-1 (RAD1-RAD9-HUS1) complex and RHNO1 onto chromatin, and in CHEK1 activation (By similarity). Involved in homologous recombination by mediating recruitment of the MRN complex to DNA damage sites (By similarity). May also serve as a sensor of DNA replication progression (By similarity). KEYWORDS: ATP-binding;Cell cycle;Chromosome;Developmental protein;DNA damage;Nucleotide-binding;Nucleus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Nucleus Chromosome Note=Phosphorylated form redistributes to discrete nuclear foci upon DNA damage. Localizes to DNA double-strand breaks (DSBs). " Q6NZB1,"PROTEIN NAMES: Protein arginine N-methyltransferase 6 (Histone-arginine N-methyltransferase PRMT6) PROTEIN FAMILY: Class I-like SAM-binding methyltransferase superfamily, Protein arginine N-methyltransferase family, PRMT6 subfamily ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N-methyltransferase family. PRMT6 subfamily. FUNCTION: Arginine methyltransferase that can catalyze the formation of both omega-N monomethylarginine (MMA) and asymmetrical dimethylarginine (aDMA), with a strong preference for the formation of aDMA. Preferentially methylates arginyl residues present in a glycine and arginine-rich domain and displays preference for monomethylated substrates (By similarity). Specifically mediates the asymmetric dimethylation of histone H3 'Arg-2' to form H3R2me2a (By similarity). H3R2me2a represents a specific tag for epigenetic transcriptional repression and is mutually exclusive with methylation on histone H3 'Lys-4' (H3K4me2 and H3K4me3) (By similarity). Acts as a transcriptional repressor of various genes such as HOXA2, THBS1 and TP53. Repression of TP53 blocks cellular senescence. Also methylates histone H2A and H4 'Arg-3' (H2AR3me and H4R3me, respectively) (By similarity). Acts as a regulator of DNA base excision during DNA repair by mediating the methylation of DNA polymerase beta (POLB), leading to the stimulation of its polymerase activity by enhancing DNA binding and processivity (By similarity). Methylates HMGA1 (By similarity). Regulates alternative splicing events (By similarity). Acts as a transcriptional coactivator of a number of steroid hormone receptors including ESR1, ESR2, PGR and NR3C1 (By similarity). Promotes fasting-induced transcriptional activation of the gluconeogenic program through methylation of the CRTC2 transcription coactivator. Methylates GPS2, protecting GPS2 from ubiquitination and degradation. Methylates SIRT7, inhibiting SIRT7 histone deacetylase activity and promoting mitochondria biogenesis (By similarity). KEYWORDS: 3D-structure;Chromatin regulator;DNA damage;DNA repair;Methylation;Methyltransferase;Nucleus;Reference proteome;Repressor;S-adenosyl-L-methionine;Transcription;Transcription regulation;Transferase SUBCELLULAR LOCATION: Nucleus " Q6P050,"PROTEIN NAMES: F-box and leucine-rich protein 22 ORGANISM: Homo sapiens (Human) FUNCTION: Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. Promotes ubiquitination of sarcomeric proteins alpha-actinin-2 (ACTN2) and filamin-C (FLNC). PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Cytoplasm;Reference proteome;Ubl conjugation pathway SUBCELLULAR LOCATION: Cytoplasm, myofibril, sarcomere, Z line " Q6P4A7,PROTEIN NAMES: Sideroflexin-4 (Breast cancer resistance marker 1) PROTEIN FAMILY: Sideroflexin family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the sideroflexin family. FUNCTION: Mitochondrial amino-acid transporter (By similarity). Does not act as a serine transporter: not able to mediate transport of serine into mitochondria. KEYWORDS: Acetylation;Alternative splicing;Amino-acid transport;Membrane;Mitochondrion;Mitochondrion inner membrane;Primary mitochondrial disease;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Mitochondrion inner membrane ; Multi-pass membrane protein Q6P4I2,"PROTEIN NAMES: WD repeat-containing protein 73 PROTEIN FAMILY: WD repeat WDR73 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the WD repeat WDR73 family. FUNCTION: May play a role in the regulation of microtubule organization and dynamics. KEYWORDS: Cytoplasm;Cytoskeleton;Epilepsy;Intellectual disability;Reference proteome;Repeat;WD repeat SUBCELLULAR LOCATION: Cytoplasm, cytosol. Cytoplasm, cytoskeleton, spindle Cytoplasm, cytoskeleton, spindle pole Cleavage furrow Note=During interphase, located in the cytosol. During mitosis, accumulates at the spindle poles and microtubule asters and later in the cleavage furrow. " Q6P4T1,PROTEIN NAMES: Sorting nexin-19 PROTEIN FAMILY: Sorting nexin family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the sorting nexin family. FUNCTION: Plays a role in intracellular vesicle trafficking and exocytosis. May play a role in maintaining insulin-containing dense core vesicles in pancreatic beta-cells and in preventing their degradation. May play a role in insulin secretion. Interacts with membranes containing phosphatidylinositol 3-phosphate (PtdIns(3P)). KEYWORDS: 3D-structure;Cytoplasmic vesicle;Endosome;Exocytosis;Lipid-binding;Membrane;Protein transport;Reference proteome;Transport SUBCELLULAR LOCATION: Early endosome membrane ; Peripheral membrane protein ; Cytoplasmic side Cytoplasmic vesicle membrane ; Peripheral membrane protein ; Cytoplasmic side Q6P6T5,"PROTEIN NAMES: Occludin PROTEIN FAMILY: ELL/occludin family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the ELL/occludin family. FUNCTION: May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. May be involved in the organization of actin in endothelial cells. KEYWORDS: Cell junction;Cell membrane;Coiled coil;Disulfide bond;Membrane;Phosphoprotein;Reference proteome;Tight junction;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Cell junction, tight junction " Q6P6V1,"PROTEIN NAMES: Polypeptide N-acetylgalactosaminyltransferase 11 (Polypeptide GalNAc transferase 11) (GalNAc-T11) (pp-GaNTase 11) (Protein-UDP acetylgalactosaminyltransferase 11) (UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 11) PROTEIN FAMILY: Glycosyltransferase 2 family, GalNAc-T subfamily ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily. FUNCTION: Polypeptide N-acetylgalactosaminyltransferase that catalyzes the initiation of protein O-linked glycosylation and is involved in left/right asymmetry by mediating O-glycosylation of NOTCH1. O-glycosylation of NOTCH1 promotes activation of NOTCH1, modulating the balance between motile and immotile (sensory) cilia at the left-right organiser (LRO). Polypeptide N-acetylgalactosaminyltransferases catalyze the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Displays the same enzyme activity toward MUC1, MUC4, and EA2 than GALNT1. Not involved in glycosylation of erythropoietin (EPO) (By similarity). PATHWAY: Protein modification; protein glycosylation. KEYWORDS: Disulfide bond;Glycoprotein;Glycosyltransferase;Golgi apparatus;Lectin;Manganese;Membrane;Metal-binding;Notch signaling pathway;Phosphoprotein;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus membrane ; Single-pass type II membrane protein " Q6P8X6,"PROTEIN NAMES: Ubiquitin carboxyl-terminal hydrolase 50 (Deubiquitinating enzyme 50) (Ubiquitin thioesterase 50) (Ubiquitin-specific-processing protease 50) PROTEIN FAMILY: Peptidase C19 family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the peptidase C19 family. FUNCTION: Deubiquitinating enzyme that removes conjugated ubiquitin from specific proteins to regulate different cellular processes. Regulates the inflammasome signaling pathway by deubiquitinating 'Lys-63'-linked polyubiquitination of the PYCARD/ASC adapter protein. Regulates the ubiquitination and stability of the ACE2 protein. Acts as a negative regulator of the G2/M checkpoint pathway, by preventing serine/threonine kinase WEE1 degradation, thereby repressing entry into mitosis following activation of the G2/M DNA damage checkpoint. KEYWORDS: Alternative splicing;Cell cycle;Cytoplasm;Cytoskeleton;Hydrolase;Nucleus;Protease;Reference proteome;Thiol protease;Ubl conjugation pathway SUBCELLULAR LOCATION: Cytoplasm Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Nucleus Note=Accumulates in the nucleus following DNA injury. " Q6PA06,"PROTEIN NAMES: Atlastin-2 (ADP-ribosylation factor-like protein 6-interacting protein 2) (ARL-6-interacting protein 2) (Aip-2) PROTEIN FAMILY: TRAFAC class dynamin-like GTPase superfamily, GB1/RHD3 GTPase family, GB1 subfamily ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the TRAFAC class dynamin-like GTPase superfamily. GB1/RHD3 GTPase family. GB1 subfamily. FUNCTION: GTPase tethering membranes through formation of trans-homooligomers and mediating homotypic fusion of endoplasmic reticulum membranes. Functions in endoplasmic reticulum tubular network biogenesis. KEYWORDS: Alternative splicing;Coiled coil;Endoplasmic reticulum;GTP-binding;Hydrolase;Membrane;Methylation;Nucleotide-binding;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein Note=Localizes at endoplasmic reticulum (ER) three-way tubular junctions. " Q6PCB0,"PROTEIN NAMES: von Willebrand factor A domain-containing protein 1 ORGANISM: Homo sapiens (Human) FUNCTION: Promotes matrix assembly (By similarity). Involved in the organization of skeletal muscles and in the formation of neuromuscular junctions (Probable). KEYWORDS: Alternative splicing;Basement membrane;Disease variant;Disulfide bond;Extracellular matrix;Glycoprotein;Neuropathy;Phosphoprotein;Reference proteome;Repeat;Secreted;Signal SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix, basement membrane " Q6PCT2,"PROTEIN NAMES: F-box/LRR-repeat protein 19 (F-box and leucine-rich repeat protein 19) ORGANISM: Homo sapiens (Human) FUNCTION: Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex that plays a role in different processes including cell migration, cell proliferation or cytoskeletal reorganization. Mediates RHOA ubiquitination and degradation in a ERK2-dependent manner. Induces RAC1 and RAC3 degradation by the proteasome system and thereby regulates TGFB1-induced E-cadherin down-regulation and cell migration. Mediates also ubiquitination and degradation of IL-33-induced receptor IL1RL1 and subsequently blocks IL-33-mediated apoptosis (By similarity). Within the nucleus, binds to DNA containing unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Recruits CDK-mediator to chromatin and targets CDK8 to promoters of silent developmental genes leading to induction of these genes during cell differentiation. In addition, plays a critical role in the recruitment of RNF20 to histone H2B leading to H2B mono-ubiquitination (By similarity). KEYWORDS: 3D-structure;Alternative splicing;Cytoplasm;DNA-binding;Leucine-rich repeat;Metal-binding;Nucleus;Reference proteome;Repeat;Ubl conjugation;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cytoplasm Nucleus " Q6PGY3,"PROTEIN NAMES: Tricarboxylate transport protein A, mitochondrial (Citrate transport protein A) (Solute carrier family 25 member 1a) PROTEIN FAMILY: Mitochondrial carrier family ORGANISM: Danio rerio (Zebrafish) (Brachydanio rerio) SIMILARITY: Belongs to the mitochondrial carrier (TC 2.A.29) family. FUNCTION: Mitochondrial electroneutral antiporter that exports citrate from the mitochondria into the cytosol in exchange for malate. Also able to mediate the exchange of citrate for isocitrate, phosphoenolpyruvate, cis-aconitate and to a lesser extend cis-aconitate, maleate and succinate (By similarity). Required for proper neuromuscular junction formation. KEYWORDS: Antiport;Membrane;Mitochondrion;Mitochondrion inner membrane;Reference proteome;Repeat;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Mitochondrion inner membrane ; Multi-pass membrane protein " Q6PQK2,"PROTEIN NAMES: Nitrophorin-7 (NP7) (Nitrite dismutase) PROTEIN FAMILY: Calycin superfamily, Nitrophorin family ORGANISM: Rhodnius prolixus (Triatomid bug) SIMILARITY: Belongs to the calycin superfamily. Nitrophorin family. FUNCTION: Converts nitrite as the sole substrate to form nitric oxide gas (NO). NO(2-) serves both as an electron donor and as an electron acceptor. Binds to negatively charged cell surfaces of activated platelets; binds to L-a-phosphatidyl-L-serine (PS)-bearing phospholipid membranes. Once bound on an activated platelet, NP7 releases its stored nitric oxide gas (NO) into the victim's tissues while feeding, resulting in vasodilation and inhibition of platelet aggregation. Also acts as an anticoagulant by blocking coagulation-factor binding sites. Has antihistamine activity; binds histamine with high affinity. KEYWORDS: 3D-structure;Disulfide bond;Heme;Iron;Metal-binding;Oxidoreductase;Reference proteome;Secreted;Signal;Vasoactive;Vasodilator SUBCELLULAR LOCATION: Secreted " Q6Q0C0,"PROTEIN NAMES: E3 ubiquitin-protein ligase TRAF7 (RING finger and WD repeat-containing protein 1) (RING finger protein 119) (RING-type E3 ubiquitin transferase TRAF7) (TNF receptor-associated factor 7) PROTEIN FAMILY: WD repeat TRAF7 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the WD repeat TRAF7 family. FUNCTION: E3 ubiquitin and SUMO-protein ligase that plays a role in different biological processes such as innate immunity, inflammation or apoptosis. Potentiates MAP3K3-mediated activation of JUN/AP1 and DDIT3 transcriptional regulators. Negatively regulates MYB transcriptional activity by sequestering it to the cytosol via SUMOylation (By similarity). Plays a role in the phosphorylation of MAPK1 and/or MAPK3, probably via its interaction with MAP3K3. Negatively regulates RLR-mediated innate immunity by promoting 'Lys-48'-linked ubiquitination of TBK1 through its RING domain to inhibit the cellular antiviral response. Promotes 'Lys-29'-linked polyubiquitination of NEMO/IKBKG and RELA leading to targeting these two proteins to lysosomal degradative pathways, reducing the transcriptional activity of NF-kappa-B. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: 3D-structure;Alternative splicing;Apoptosis;Cytoplasm;Cytoplasmic vesicle;Disease variant;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Repeat;Transcription;Transcription regulation;Transferase;Ubl conjugation;Ubl conjugation pathway;WD repeat;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cytoplasmic vesicle Cytoplasm Nucleus Note=Colocalizes with MAP3K3 to vesicle-like structures throughout the cytoplasm. " Q6R2K3,"PROTEIN NAMES: Protein STRUBBELIG-RECEPTOR FAMILY 3 (Leucine-rich repeat receptor kinase-like protein SRF3) PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. FUNCTION: Not essential for epidermal patterning and not redundant with STRUBBELIG. KEYWORDS: Alternative splicing;ATP-binding;Glycoprotein;Leucine-rich repeat;Membrane;Nucleotide-binding;Receptor;Reference proteome;Repeat;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Multi-pass membrane protein MISCELLANEOUS: Over-expression of SRF3 led to male-sterility in both cv. Landsberg and cv. Columbia." Q6R5N8,"PROTEIN NAMES: Toll-like receptor 13 PROTEIN FAMILY: Toll-like receptor family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the Toll-like receptor family. FUNCTION: Component of innate and adaptive immunity that recognizes and binds 23S rRNA from bacteria. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Specifically binds the 5'-CGGAAAGACC-3' sequence on bacterial 23S rRNA, a sequence also bound by MLS group antibiotics (including erythromycin). May also recognize vesicular stomatitis virus; however, these data require additional evidences. KEYWORDS: 3D-structure;Endosome;Glycoprotein;Immunity;Inflammatory response;Innate immunity;Leucine-rich repeat;Membrane;NAD;Receptor;Reference proteome;Repeat;RNA-binding;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endosome membrane ; Single-pass type I membrane protein MISCELLANEOUS: The sequence 23S rRNA from clinical isolates of erythromycin-resistant S.aureus is methylated and is not recognized by Tlr13 anymore, suggesting a link between antibiotic resistance and evasion from Tlr13 recognition. 23S rRNA modifications generating resistance toward MLS antibiotics preventing recognition of bacteria from Tlr13. These data may also explain why Tlr13 is not conserved in human: human may instead possess a related rRNA-sensing pattern recognition receptor that has evolved to recognize species that can hide from Tlr13 owing to rRNA modifications." Q6R7M4,"PROTEIN NAMES: Methyl farnesoate epoxidase (Cytochrome P450 CYP15A1) PROTEIN FAMILY: Cytochrome P450 family ORGANISM: Diploptera punctata (Pacific beetle cockroach) SIMILARITY: Belongs to the cytochrome P450 family. FUNCTION: Catalyzes the conversion of methyl farnesoate to juvenile hormone III acid (methyl (2E,6E)-(10R)-10,11-epoxy-3,7,11-trimethyl-2,6-dodecadienoate) in juvenile hormone biosynthesis. KEYWORDS: Glycoprotein;Heme;Iron;Metal-binding;Monooxygenase;Oxidoreductase;Signal MISCELLANEOUS: The enzyme is present in all insects, except in lepidoptera (moths and butterflies), and is specific for methyl farnesoate. Lepidoptera contain the farnesoate epoxidase, which is specific for farnesoate." Q6S5C2,"PROTEIN NAMES: N-acetylglucosamine-1-phosphotransferase subunit gamma (GlcNAc-1-phosphotransferase subunit gamma) (M6PR domain-containing protein 1) (UDP-N-acetylglucosamine-1-phosphotransferase subunit gamma) ORGANISM: Mus musculus (Mouse) FUNCTION: Non-catalytic subunit of the N-acetylglucosamine-1-phosphotransferase complex, an enzyme that catalyzes the formation of mannose 6-phosphate (M6P) markers on high mannose type oligosaccharides in the Golgi apparatus. Binds and presents the high mannose glycans of the acceptor to the catalytic alpha and beta subunits (GNPTAB). Enhances the rate of N-acetylglucosamine-1-phosphate transfer to the oligosaccharides of acid hydrolase acceptors. KEYWORDS: Alternative splicing;Disulfide bond;Glycoprotein;Golgi apparatus;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted. Golgi apparatus. " Q6SJ93,PROTEIN NAMES: Serine protease FAM111B (Cancer-associated nucleoprotein) PROTEIN FAMILY: FAM111 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the FAM111 family. FUNCTION: Serine protease. KEYWORDS: Acetylation;Alternative splicing;Disease variant;Hydrolase;Isopeptide bond;Protease;Reference proteome;Ubl conjugation Q6STH5,"PROTEIN NAMES: Fe-S cluster assembly factor HCF101, chloroplastic (Protein HIGH CHLOROPHYLL FLUORESCENCE 101) PROTEIN FAMILY: Mrp/NBP35 ATP-binding proteins family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the Mrp/NBP35 ATP-binding proteins family. FUNCTION: Required for photosystem I (PSI) biosynthesis and assembly. May serve as a chloroplast scaffold protein that specifically assembles iron-sulfur (4Fe-4S) clusters and transfers them to the chloroplast PSI and ferredoxin-thioredoxin (FTR) complexes. Can assemble a 4Fe-4S cluster and transfer it to apoproteins in yeast cells. Probably not required for assembly or stability of plastidic 2Fe-2S clusters. KEYWORDS: Acetylation;ATP-binding;Chloroplast;Iron;Iron-sulfur;Metal-binding;Nucleotide-binding;Plastid;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast stroma " Q6TMK4,PROTEIN NAMES: Peroxinectin A PROTEIN FAMILY: Peroxidase family ORGANISM: Dictyostelium discoideum (Social amoeba) SIMILARITY: Belongs to the peroxidase family. KEYWORDS: 3D-structure;Glycoprotein;Hydrogen peroxide;Oxidoreductase;Peroxidase;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted Q6TNA5,"PROTEIN NAMES: L-allo-isoleucine:holo-[CmaA peptidyl-carrier protein] ligase (Adenylation activation enzyme) (Coronamic acid synthetase CmaA) PROTEIN FAMILY: ATP-dependent AMP-binding enzyme family ORGANISM: Pseudomonas savastanoi pv. glycinea (Pseudomonas syringae pv. glycinea) SIMILARITY: Belongs to the ATP-dependent AMP-binding enzyme family. FUNCTION: Involved in the biosynthesis of the phytotoxin coronatine (COR) which mimics the plant hormone jasmonic acid isoleucine and promotes opening of stomata for bacterial entry, bacterial growth in the apoplast, systemic susceptibility, and disease symptoms. CmaA catalyzes the adenylation of L-allo-isoleucine (via the A domain) and the attachment of L-allo-isoleucine to the 4'-phosphopantetheine arm located within the T domain of CmaA. It can also use L-isoleucine, L-leucine and L-valine as substrates. KEYWORDS: Ligase;Phosphopantetheine;Phosphoprotein;Plasmid;Virulence " Q6U6J0,"PROTEIN NAMES: Juvenile hormone epoxide hydrolase (bommo-JHEH) PROTEIN FAMILY: Peptidase S33 family ORGANISM: Bombyx mori (Silk moth) SIMILARITY: Belongs to the peptidase S33 family. FUNCTION: Catalyzes juvenile hormone hydrolysis. Degrades juvenile hormone III (JH III) about 3 times and 5 times slower than juvenile hormone I (JH I) and II (JH II), respectively. Degrades cis-stilbene oxide and trans-stilbene oxide about 18 and 43 times slower than JH III, respectively. KEYWORDS: 3D-structure;Aromatic hydrocarbons catabolism;Endoplasmic reticulum;Hydrolase;Membrane;Microsome;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Microsome membrane ; Single-pass membrane protein Endoplasmic reticulum membrane ; Single-pass membrane protein " Q6U7R4,"PROTEIN NAMES: T-cell immunoglobulin and mucin domain-containing protein 4 (TIMD-4) (Spleen, mucin-containing, knockout of lymphotoxin protein) (SMUCKLER) (T-cell immunoglobulin mucin receptor 4) (TIM-4) (T-cell membrane protein 4) PROTEIN FAMILY: Immunoglobulin superfamily, TIM family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the immunoglobulin superfamily. TIM family. FUNCTION: Phosphatidylserine receptor that plays different role in immune response including phagocytosis of apoptotic cells and T-cell regulation. Controls T-cell activation in a bimodal fashion, decreasing the activation of naive T-cells by inducing cell cycle arrest, while increasing proliferation of activated T-cells by activating AKT1 and ERK1/2 phosphorylations and subsequent signaling pathways. Also plays a role in efferocytosis which is the process by which apoptotic cells are removed by phagocytic cells. Mechanistically, promotes the engulfment of apoptotic cells or exogenous particles by securing them to phagocytes through direct binding to phosphatidylserine present on apoptotic cells, while other engulfment receptors such as MERTK efficiently recognize apoptotic cells and mediate their ingestion (By similarity). Additionally, promotes autophagy process by suppressing NLRP3 inflammasome activity via activation of STK11/PRKAA1 pathway in a phosphatidylserine-dependent mechanism. KEYWORDS: 3D-structure;Disulfide bond;Glycoprotein;Immunoglobulin domain;Membrane;Phosphoprotein;Reference proteome;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Single-pass type I membrane protein MISCELLANEOUS: Belongs to the T-cell and airway phenotype regulator (Tapr) locus, a single chromosomal region that confers reduced T-helper type 2 responsiveness and protects against airway hyperactivity (AHR), the hallmark of human asthma.; MISCELLANEOUS: Low expression of Timd4 is associated with lymphotoxin deficiency." Q6UC88,"PROTEIN NAMES: Lymphatic vessel endothelial hyaluronic acid receptor 1 (LYVE-1) (Cell surface retention sequence-binding protein 1) (CRSBP-1) (Extracellular link domain-containing protein 1) ORGANISM: Bos taurus (Bovine) FUNCTION: Ligand-specific transporter trafficking between intracellular organelles (TGN) and the plasma membrane. Plays a role in autocrine regulation of cell growth mediated by growth regulators containing cell surface retention sequence binding (CRS). May act as a hyaluronan (HA) transporter, either mediating its uptake for catabolism within lymphatic endothelial cells themselves, or its transport into the lumen of afferent lymphatic vessels for subsequent re-uptake and degradation in lymph nodes. Binds to pericelluar hyaluronan matrices deposited on the surface of leukocytes and facilitates cell adhesion and migration through lymphatic endothelium (By similarity). KEYWORDS: Disulfide bond;Glycoprotein;Membrane;Receptor;Reference proteome;Signal;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Membrane ; Single-pass membrane protein Note=Localized to the plasma membrane and in vesicles near extranuclear membranes which may represent trans-Golgi network (TGN) and endosomes/prelysosomeal compartments. Undergoes ligand-dependent internalization and recycling at the cell surface (By similarity). " Q6UWB1,"PROTEIN NAMES: Interleukin-27 receptor subunit alpha (IL-27 receptor subunit alpha) (IL-27R subunit alpha) (IL-27R-alpha) (IL-27RA) (Cytokine receptor WSX-1) (Cytokine receptor-like 1) (Type I T-cell cytokine receptor) (TCCR) (ZcytoR1) PROTEIN FAMILY: Type I cytokine receptor family, Type 2 subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the type I cytokine receptor family. Type 2 subfamily. FUNCTION: Receptor for IL27. Requires IL6ST/GP130 to mediate signal transduction in response to IL27. This signaling system acts through STAT3 and STAT1. Acts as a receptor for the neuroprotective peptide humanin as part of a complex with IL6ST/GP130 and CNTFR. Involved in the regulation of Th1-type immune responses. Also appears to be involved in innate defense mechanisms. KEYWORDS: 3D-structure;Glycoprotein;Membrane;Receptor;Reference proteome;Repeat;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. " Q6UWB4,"PROTEIN NAMES: Serine protease 55 (Testis serine protease 1) (T-SP1) PROTEIN FAMILY: Peptidase S1 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the peptidase S1 family. FUNCTION: Probable serine protease, which plays a crucial role in the fertility of male mice including sperm migration and sperm-egg interaction. KEYWORDS: Alternative splicing;Cell membrane;Cytoplasm;Disulfide bond;Glycoprotein;GPI-anchor;Hydrolase;Lipoprotein;Membrane;Protease;Reference proteome;Serine protease;Signal SUBCELLULAR LOCATION: [Isoform 1]: Cell membrane ; Lipid-anchor, GPI-anchor Cytoplasm, cytosol Note=Mainly found in the membrane part of the cells and only in small amounts in the cytosol.; SUBCELLULAR LOCATION: [Isoform 2]: Cytoplasm, cytosol Note=Present primarily in the cytosol and only in minor amounts in the membrane fraction. " Q6UX06,"PROTEIN NAMES: Olfactomedin-4 (OLM4) (Antiapoptotic protein GW112) (G-CSF-stimulated clone 1 protein) (hGC-1) (hOLfD) ORGANISM: Homo sapiens (Human) FUNCTION: May promote proliferation of pancreatic cancer cells by favoring the transition from the S to G2/M phase. In myeloid leukemic cell lines, inhibits cell growth and induces cell differentiation and apoptosis. May play a role in the inhibition of EIF4EBP1 phosphorylation/deactivation. Facilitates cell adhesion, most probably through interaction with cell surface lectins and cadherin. KEYWORDS: Cell adhesion;Coiled coil;Disulfide bond;Glycoprotein;Mitochondrion;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted, extracellular space. Mitochondrion. Note=Subcellular location is not clearly defined: has been shown to be secreted , but also in the mitochondrion , cytoplasm and plasma membrane and in the nucleus. " Q6UX15,PROTEIN NAMES: Layilin ORGANISM: Homo sapiens (Human) FUNCTION: Receptor for hyaluronate. KEYWORDS: Alternative splicing;Disulfide bond;Glycoprotein;Lectin;Membrane;Phosphoprotein;Reference proteome;Repeat;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Single-pass type I membrane protein Note=Colocalizes with TLN1 at the membrane ruffles. Q6UX41,"PROTEIN NAMES: Butyrophilin-like protein 8 PROTEIN FAMILY: Immunoglobulin superfamily, BTN/MOG family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the immunoglobulin superfamily. BTN/MOG family. FUNCTION: May stimulate primary immune response. Acts on T-cell stimulated sub-optimally through the TCR/CD3 complex stimulating their proliferation and cytokine production. KEYWORDS: Adaptive immunity;Alternative splicing;Direct protein sequencing;Disulfide bond;Glycoprotein;Immunity;Immunoglobulin domain;Membrane;Reference proteome;Repeat;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Single-pass type I membrane protein " Q6UX46,"PROTEIN NAMES: ALK and LTK ligand 2 (Augmentor alpha) (AUG-alpha) PROTEIN FAMILY: ALKAL family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the ALKAL family. FUNCTION: Cytokine that acts as a physiological ligand for receptor tyrosine kinases LTK and ALK, leading to their activation. Cytokine-binding is sufficient to activate LTK. In contrast, ALKAL2-driven activation of ALK is coupled with heparin-binding to ALK. Stimulation of ALK signaling is involved in neural development and regulation of energy expenditure. KEYWORDS: 3D-structure;Alternative splicing;Cell membrane;Cytokine;Direct protein sequencing;Disulfide bond;Membrane;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted Cell membrane Note=Following interaction with receptor tyrosine kinase ALK, associates with the cell membrane, membrane-binding is required to activate ALK. " Q6UY09,"PROTEIN NAMES: Carcinoembryonic antigen-related cell adhesion molecule 20 PROTEIN FAMILY: Immunoglobulin superfamily, CEA family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the immunoglobulin superfamily. CEA family. FUNCTION: Together with the tyrosine-protein kinase SYK, enhances production of the cytokine CXCL8/IL-8 via the NFKB pathway and may thus have a role in the intestinal immune response. KEYWORDS: Alternative splicing;Cell membrane;Cell projection;Disulfide bond;Glycoprotein;Immunity;Immunoglobulin domain;Membrane;Phosphoprotein;Reference proteome;Repeat;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell projection, microvillus membrane ; Single-pass type I membrane protein Apical cell membrane ; Single-pass type I membrane protein Note=Colocalizes with PTPRH and CEACAM1 at the apical brush border of intestinal cells. " Q6V0L0,"PROTEIN NAMES: Cytochrome P450 26C1 (CYP26C1) PROTEIN FAMILY: Cytochrome P450 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the cytochrome P450 family. FUNCTION: A cytochrome P450 monooxygenase involved in the metabolism of retinoates (RAs), the active metabolites of vitamin A, and critical signaling molecules in animals. RAs exist as at least four different isomers: all-trans-RA (atRA), 9-cis-RA, 13-cis-RA, and 9,13-dicis-RA, where atRA is considered to be the biologically active isomer, although 9-cis-RA and 13-cis-RA also have activity (Probable). Catalyzes the oxidation of atRA primarily at C-4. Oxidation of atRA limits its biological activity and initiates a degradative process leading to its eventual elimination, thereby contributes to the regulation of atRA homeostasis and signaling (Probable). Able to metabolize other RAs such as 9-cis with high efficiency. Can oxidize all-trans-13,14-dihydroretinoate (DRA) to metabolites which could include all-trans-4-oxo-DRA, all-trans-4-hydroxy-DRA, all-trans-5,8-epoxy-DRA, and all-trans-18-hydroxy-DRA (By similarity). Shares sequence similarity with other CYP26 family members, but has higher affinity to 9-cis-RA and is much less sensitive to the inhibitory effects of ketoconazole. In cooperation with Cyp26a1, contributes to the CNS patterning and the development of regions of higher visual acuity (By similarity). KEYWORDS: Disease variant;Ectodermal dysplasia;Heme;Iron;Lipid metabolism;Membrane;Metal-binding;Monooxygenase;Oxidoreductase;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Single-pass membrane protein " Q6V1X1,"PROTEIN NAMES: Dipeptidyl peptidase 8 (DP8) (Dipeptidyl peptidase IV-related protein 1) (DPRP-1) (Dipeptidyl peptidase VIII) (DPP VIII) (Prolyl dipeptidase DPP8) PROTEIN FAMILY: Peptidase S9B family, DPPIV subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the peptidase S9B family. DPPIV subfamily. FUNCTION: Dipeptidyl peptidase that cleaves off N-terminal dipeptides from proteins having a Pro or Ala residue at position 2. Acts as a key inhibitor of caspase-1-dependent monocyte and macrophage pyroptosis in resting cells by preventing activation of NLRP1 and CARD8. Sequesters the cleaved C-terminal part of NLRP1 and CARD8, which respectively constitute the active part of the NLRP1 and CARD8 inflammasomes, in a ternary complex, thereby preventing their oligomerization and activation. The dipeptidyl peptidase activity is required to suppress NLRP1 and CARD8; however, neither NLRP1 nor CARD8 are bona fide substrates of DPP8, suggesting the existence of substrate(s) required for NLRP1 and CARD8 inhibition (By similarity). KEYWORDS: 3D-structure;Alternative splicing;Aminopeptidase;Apoptosis;Cytoplasm;Hydrolase;Protease;Reference proteome;Serine protease SUBCELLULAR LOCATION: Cytoplasm " Q6V9Y8,"PROTEIN NAMES: Glypican-3 [Cleaved into: Glypican-3 alpha subunit; Glypican-3 beta subunit] PROTEIN FAMILY: Glypican family ORGANISM: Danio rerio (Zebrafish) (Brachydanio rerio) SIMILARITY: Belongs to the glypican family. FUNCTION: Cell surface proteoglycan (By similarity). Negatively regulates the hedgehog signaling pathway (By similarity). Positively regulates the canonical and non-canonical Wnt signaling pathways (By similarity). Binds to CD81 which decreases the availability of free CD81 for binding to the transcriptional repressor HHEX, resulting in nuclear translocation of HHEX and transcriptional repression (By similarity). Inhibits the dipeptidyl peptidase activity of DPP4 (By similarity). Plays a role in limb patterning and skeletal development (By similarity). Modulates the effects of growth factors on renal branching morphogenesis (By similarity). Required for coronary vascular development (By similarity). Plays a role in regulating cell movements during gastrulation. KEYWORDS: Cell membrane;Disulfide bond;Glycoprotein;GPI-anchor;Heparan sulfate;Lipoprotein;Membrane;Proteoglycan;Reference proteome;Signal SUBCELLULAR LOCATION: Cell membrane ; Lipid-anchor, GPI-anchor ; Extracellular side " Q6VE93,"PROTEIN NAMES: Serine/threonine-protein acetyltransferase HopZ1a (Protein orf34) PROTEIN FAMILY: Acetyltransferase YopJ family ORGANISM: Pseudomonas syringae pv. syringae SIMILARITY: Belongs to the acetyltransferase YopJ family. FUNCTION: Serine/threonine-protein acetyltransferase translocated into infected cells, which impairs host microtubule network and host immunity by mediating acetylation of target proteins. Blocks secretion in host cells by mediating acetylation of host tubulin, thereby impairing host microbubule network. Impairs host cell immunity by mediating acetylation of host TIFY/JAZ transcription repressors (Arabidopsis thaliana TIFY10B/JAZ2, TIFY11A/JAZ5, TIFY11B/JAZ6, TIFY5A/JAZ8, TIFY9/JAZ10 and TIFY3B/JAZ12), thereby activating host jasmonate signaling. KEYWORDS: 3D-structure;Acetylation;Acyltransferase;Allosteric enzyme;Host cell membrane;Host cytoplasm;Host cytoskeleton;Host membrane;Host nucleus;Membrane;Plasmid;Secreted;Transferase;Virulence SUBCELLULAR LOCATION: Secreted Host cell membrane Host cytoplasm, host cytoskeleton Host nucleus Note=Secreted via type III secretion system (T3SS). " Q6WKZ7,"PROTEIN NAMES: Nostrin (Disabled homolog 2-interacting protein 2) (Dab2-interacting protein 2) (Nitric oxide synthase trafficker) (eNOS-trafficking inducer) ORGANISM: Mus musculus (Mouse) FUNCTION: Multivalent adapter protein which may decrease NOS3 activity by inducing its translocation away from the plasma membrane. KEYWORDS: Cell membrane;Coiled coil;Cytoplasm;Cytoplasmic vesicle;Cytoskeleton;Endocytosis;Membrane;Nucleus;Phosphoprotein;Reference proteome;SH3 domain SUBCELLULAR LOCATION: Cell membrane ; Peripheral membrane protein ; Cytoplasmic side Cytoplasmic vesicle Cytoplasm, cytoskeleton Cytoplasm Nucleus Note=Enriched in selected actin structures. MISCELLANEOUS: Seems to repress its own transcription." Q6WN34,"PROTEIN NAMES: Chordin-like protein 2 (Breast tumor novel factor 1) (BNF-1) (Chordin-related protein 2) ORGANISM: Homo sapiens (Human) FUNCTION: May inhibit BMPs activity by blocking their interaction with their receptors. Has a negative regulator effect on the cartilage formation/regeneration from immature mesenchymal cells, by preventing or reducing the rate of matrix accumulation (By similarity). Implicated in tumor angiogenesis. May play a role during myoblast and osteoblast differentiation, and maturation. KEYWORDS: Alternative splicing;Chondrogenesis;Cytoplasm;Developmental protein;Differentiation;Direct protein sequencing;Glycoprotein;Osteogenesis;Phosphoprotein;Reference proteome;Repeat;Secreted;Signal SUBCELLULAR LOCATION: [Isoform 1]: Secreted.; SUBCELLULAR LOCATION: [Isoform 2]: Secreted.; SUBCELLULAR LOCATION: [Isoform 3]: Cytoplasm.; SUBCELLULAR LOCATION: [Isoform 4]: Cytoplasm.; SUBCELLULAR LOCATION: [Isoform 5]: Cytoplasm " Q6WUC2,"PROTEIN NAMES: (R,S)-reticuline 7-O-methyltransferase (7OMT) PROTEIN FAMILY: Class I-like SAM-binding methyltransferase superfamily, Cation-independent O-methyltransferase family ORGANISM: Papaver somniferum (Opium poppy) SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O-methyltransferase family. FUNCTION: Catalyzes the transfer of a methyl group to reticuline to form laudanine. Methylates the simple catechols guaiacol and isovanillic acid as well as the tetrahydrobenzylisoquinolines (R)-reticuline, (S)-reticuline, (R,S)-orientaline, (R)-protosinomenine and (R,S)-isoorientaline. Involved in the production of laudanine. KEYWORDS: Alkaloid metabolism;Direct protein sequencing;Methyltransferase;S-adenosyl-L-methionine;Transferase " Q6X7J4,"PROTEIN NAMES: WUSCHEL-related homeobox 9 (Protein STIMPY) PROTEIN FAMILY: WUS homeobox family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the WUS homeobox family. FUNCTION: Homeodomain transcription factor required for meristem growth and early development. Promotes cell proliferation and prevents premature differentiation in meristematic tissues during postembryonic development. Essential for maintaining tissue growth during embryogenesis. May act by repressing TSS to promote meristematic proliferation. Involved in the transcriptional activation of a subset of cytokinin response factors. May act as a negative regulator of cytokinin signaling in the dark. KEYWORDS: Cytoplasm;Developmental protein;DNA-binding;Homeobox;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus Cytoplasm MISCELLANEOUS: The seedling growth arrest phenotype of the stip mutants likely resulted from a G2 cell cycle arrest of the meristematic tissue. Exogenous sucrose in the growth medium reactivate the stip meristems by repressing TSS and activating the expression of cell cycle regulators, and thus promoting G2 to M transition in meristematic tissues." Q6X7J5,"PROTEIN NAMES: WUSCHEL-related homeobox 8 (Protein STIMPY-LIKE) PROTEIN FAMILY: WUS homeobox family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the WUS homeobox family. FUNCTION: Probable transcription factor, which may be involved in embryonic patterning. May be required for basal embryo development after fertilization. Acts partially redundantly with STIP in promoting embryonic cell division and proliferation. Promotes cotyledon boundary formation by maintaining the symmetry in CUC genes expression domains. KEYWORDS: Developmental protein;DNA-binding;Homeobox;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " Q6XKD3,"PROTEIN NAMES: Prostaglandin D2 receptor 2 (G protein-coupled receptor 44) PROTEIN FAMILY: G-protein coupled receptor 1 family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the G-protein coupled receptor 1 family. FUNCTION: Receptor for prostaglandin D2 (PGD2). Coupled to the G(i)-protein. Receptor activation may result in pertussis toxin-sensitive decreases in cAMP levels and Ca(2+) mobilization. PI3K signaling is also implicated in mediating PTGDR2 effects. PGD2 induced receptor internalization. CRTH2 internalization can be regulated by diverse kinases such as, PKC, PKA, GRK2, GPRK5/GRK5 and GRK6. Receptor activation is responsible, at least in part, in immune regulation and allergic/inflammation responses (By similarity). KEYWORDS: Cell membrane;Disulfide bond;G-protein coupled receptor;Glycoprotein;Membrane;Phosphoprotein;Receptor;Reference proteome;Transducer;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Note=Internalized receptors colocalized with RAB11A. " Q6XR72,"PROTEIN NAMES: Calcium/manganese antiporter SLC30A10 (Solute carrier family 30 member 10) (Zinc transporter 10) (ZnT-10) PROTEIN FAMILY: Cation diffusion facilitator (CDF) transporter family, SLC30A subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. SLC30A subfamily. FUNCTION: Calcium:manganese antiporter of the plasma membrane mediating the efflux of intracellular manganese coupled to an active extracellular calcium exchange. Required for intracellular manganese homeostasis, an essential cation for the function of several enzymes, including some crucially important for the metabolism of neurotransmitters and other neuronal metabolic pathways. Manganese can also be cytotoxic and induce oxidative stress, mitochondrial dysfunction and apoptosis. Could also have an intracellular zinc ion transporter activity, directly regulating intracellular zinc ion homeostasis and more indirectly various signaling pathway and biological processes. KEYWORDS: Alternative splicing;Antiport;Cell membrane;Disease variant;Dystonia;Endosome;Golgi apparatus;Ion transport;Manganese;Membrane;Neurodegeneration;Parkinsonism;Reference proteome;Transmembrane;Transmembrane helix;Transport;Zinc;Zinc transport SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Golgi apparatus membrane ; Multi-pass membrane protein Recycling endosome membrane Early endosome membrane ; Multi-pass membrane protein Note=Localization to the Golgi and plasma membrane is regulated by zinc. MISCELLANEOUS: [Isoform 2]: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay." Q6XZB0,"PROTEIN NAMES: Lipase member I (LIPI) (Cancer/testis antigen 17) (CT17) (LPD lipase) (Membrane-associated phosphatidic acid-selective phospholipase A1-beta) (mPA-PLA1 beta) PROTEIN FAMILY: AB hydrolase superfamily, Lipase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the AB hydrolase superfamily. Lipase family. FUNCTION: Hydrolyzes specifically phosphatidic acid (PA) to produce 2-acyl lysophosphatidic acid (LPA; a potent bioactive lipid mediator) and fatty acid. Does not hydrolyze other phospholipids, like phosphatidylserine (PS), phosphatidylcholine (PC) and phosphatidylethanolamine (PE) or triacylglycerol (TG). KEYWORDS: Alternative splicing;Cell membrane;Disease variant;Disulfide bond;Glycoprotein;Heparin-binding;Hydrolase;Lipid degradation;Lipid metabolism;Membrane;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: [Isoform 1]: Cell membrane Secreted Note=May associate with lipid draft.; SUBCELLULAR LOCATION: [Isoform 2]: Cell membrane Secreted Note=May associate with lipid draft. " Q6Z955,"PROTEIN NAMES: Cytokinin dehydrogenase 11 (Cytokinin oxidase 11) (OsCKX11) PROTEIN FAMILY: Oxygen-dependent FAD-linked oxidoreductase family ORGANISM: Oryza sativa subsp. japonica (Rice) SIMILARITY: Belongs to the oxygen-dependent FAD-linked oxidoreductase family. FUNCTION: Catalyzes the oxidation of cytokinins, a family of N(6)-substituted adenine derivatives that are plant hormones, where the substituent is an isopentenyl group. Catalyzes the oxidation of various types of cytokinins in vitro with high efficiency toward trans-zeatin (tZ) and cis-zeatin (cZ), and lower efficiency toward isopentenyladenine (iP), cis-zeatin riboside (cZR), dihydrozeatin (DHZ), and isopentenyladenine riboside (iPR). Involved in the degradation of cytosolic cytokinins. Involved in the regulation of photosynthesis and grain number, thereby coordinating the source-sink relationship simultaneously. KEYWORDS: Cytoplasm;FAD;Flavoprotein;Oxidoreductase;Reference proteome SUBCELLULAR LOCATION: Cytoplasm, cytosol " Q6ZJK7,"PROTEIN NAMES: Tryptophan decarboxylase 1 (5-hydroxytryptophan decarboxylase TDC1) PROTEIN FAMILY: Group II decarboxylase family ORGANISM: Oryza sativa subsp. japonica (Rice) SIMILARITY: Belongs to the group II decarboxylase family. FUNCTION: Involved in serotonin biosynthesis. Catalyzes the decarboxylation of L-tryptophan to produce tryptamine, which is converted to serotonin by tryptamine 5-hydroxylase. May play a major role in serotonin biosynthesis during senescence. Accumulation of serotonin attenuates leaf senescence. Catalyzes the decarboxylation of 5-hydroxy-L-tryptophan to produce serotonin. KEYWORDS: 3D-structure;Decarboxylase;Lyase;Pyridoxal phosphate;Reference proteome;Serotonin biosynthesis " Q6ZJW8,"PROTEIN NAMES: Polycomb group protein FIE1 (Protein FERTILIZATION-INDEPENDENT ENDOSPERM 1) (OsFIE1) (WD40 repeat-containing protein 154) (OsWD40-154) PROTEIN FAMILY: WD repeat ESC family ORGANISM: Oryza sativa subsp. japonica (Rice) SIMILARITY: Belongs to the WD repeat ESC family. FUNCTION: Polycomb group (PcG) protein. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development. PcG proteins are not required to initiate repression, but to maintain it during later stages of development. They act via the methylation of histones, rendering chromatin heritably changed in its expressibility. Together with EZ1 and CLF forms a complex that is involved in gene transcriptional repression by trimethylation on histone H3 'Lys-27' (H3K27me3) of target genes. Involved in the regulation of embryo and seed endosperm development. FIE1-containing PcG complex in seed endosperm regulates the expression of various transcription factors by trimethylation on histone H3 'Lys-27' (H3K27me3) of target genes. Involved in the overall expression regulation of nutrient metabolism genes, such as prolamin synthesis and seed storage protein synthesis genes. Can regulate valine, leucine and isoleucine metabolism-related genes. KEYWORDS: Chromatin regulator;Developmental protein;Differentiation;Reference proteome;Repeat;Transcription;Transcription regulation;WD repeat MISCELLANEOUS: The FIE1 locus is imprinted. Maternal inherited gene is expressed in the ovule (the egg and the central cell), while the paternal inherited gene is silenced in the pollen. After fertilization, only the maternal inherited allele is expressed. The gain-of-function epi-allele (Epi-df) plants exhibit dwarf phenotype, defect in flower development and very poor seed set. Plants silencing FIE1 have delayed embryo development, reduced seed set, and reduced grain length, width and weight." Q6ZMI3,"PROTEIN NAMES: Gliomedin [Cleaved into: Gliomedin shedded ectodomain] ORGANISM: Homo sapiens (Human) FUNCTION: Ligand for NRCAM and NFASC/neurofascin that plays a role in the formation and maintenance of the nodes of Ranvier on myelinated axons. Mediates interaction between Schwann cell microvilli and axons via its interactions with NRCAM and NFASC. Nodes of Ranvier contain clustered sodium channels that are crucial for the saltatory propagation of action potentials along myelinated axons. During development, nodes of Ranvier are formed by the fusion of two heminodes. Required for normal clustering of sodium channels at heminodes; not required for the formation of mature nodes with normal sodium channel clusters. Required, together with NRCAM, for maintaining NFASC and sodium channel clusters at mature nodes of Ranvier. KEYWORDS: 3D-structure;Alternative splicing;Cell membrane;Cell projection;Collagen;Developmental protein;Differentiation;Disease variant;Extracellular matrix;Glycoprotein;Membrane;Neurogenesis;Reference proteome;Repeat;Secreted;Signal-anchor;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type II membrane protein Cell projection, axon Note=Detected at the nodes of Ranvier. Detected at immature heminodes.; SUBCELLULAR LOCATION: [Gliomedin shedded ectodomain]: Secreted Secreted, extracellular space, extracellular matrix Note=Proteolytic processing gives rise to a soluble extracellular domain that is secreted. The gliomedin shedded ectodomain localizes to the nodes of Ranvier. " Q6ZMM2,"PROTEIN NAMES: ADAMTS-like protein 5 (ADAMTSL-5) (Thrombospondin type-1 domain-containing protein 6) ORGANISM: Homo sapiens (Human) FUNCTION: May play a role in modulation of fibrillin microfibrils in the extracellular matrix (ECM). KEYWORDS: Alternative splicing;Disulfide bond;Extracellular matrix;Glycoprotein;Heparin-binding;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted Secreted, extracellular space, extracellular matrix Note=Colocalized with fibrillin microfibrils. Predominantly distributed in baso-lateral regions of fibroblast extracellular matrix. MISCELLANEOUS: [Isoform 1]: Major.; MISCELLANEOUS: [Isoform 2]: Minor." Q6ZMT1,"PROTEIN NAMES: SH3 and cysteine-rich domain-containing protein 2 (24b2/STAC2) (Src homology 3 and cysteine-rich domain-containing protein 2) ORGANISM: Homo sapiens (Human) FUNCTION: Plays a redundant role in promoting the expression of calcium channel CACNA1S at the cell membrane, and thereby contributes to increased channel activity. Slows down the inactivation rate of the calcium channel CACNA1C. KEYWORDS: 3D-structure;Alternative splicing;Cell membrane;Cytoplasm;Membrane;Metal-binding;Phosphoprotein;Reference proteome;Repeat;SH3 domain;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cytoplasm, cytosol Cell membrane ; Peripheral membrane protein ; Cytoplasmic side Cell membrane, sarcolemma ; Peripheral membrane protein ; Cytoplasmic side Note=Colocalizes with CACNA1C at the plasma membrane of transfected cells. " Q6ZN18,"PROTEIN NAMES: Zinc finger protein AEBP2 (Adipocyte enhancer-binding protein 2) (AE-binding protein 2) PROTEIN FAMILY: AEBP2/jing C2H2-type zinc-finger family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the AEBP2/jing C2H2-type zinc-finger family. FUNCTION: Acts as an accessory subunit for the core Polycomb repressive complex 2 (PRC2), which mediates histone H3K27 (H3K27me3) trimethylation on chromatin leading to transcriptional repression of the affected target gene. Plays a role in nucleosome localization of the PRC2 complex. KEYWORDS: 3D-structure;Acetylation;Alternative splicing;Chromatin regulator;DNA-binding;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Repeat;Repressor;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Note=Localizes to chromatin as part of the PRC2 complex. " Q6ZNA4,"PROTEIN NAMES: E3 ubiquitin-protein ligase Arkadia (RING finger protein 111) (hRNF111) (RING-type E3 ubiquitin transferase Arkadia) PROTEIN FAMILY: Arkadia family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the Arkadia family. FUNCTION: E3 ubiquitin-protein ligase. Required for mesoderm patterning during embryonic development (By similarity). Acts as an enhancer of the transcriptional responses of the SMAD2/SMAD3 effectors, which are activated downstream of BMP. Acts by mediating ubiquitination and degradation of SMAD inhibitors such as SMAD7, inducing their proteasomal degradation and thereby enhancing the transcriptional activity of TGF-beta and BMP. In addition to enhance transcription of SMAD2/SMAD3 effectors, also regulates their turnover by mediating their ubiquitination and subsequent degradation, coupling their activation with degradation, thereby ensuring that only effectors 'in use' are degraded (By similarity). Activates SMAD3/SMAD4-dependent transcription by triggering signal-induced degradation of SNON isoform of SKIL. Associates with UBE2D2 as an E2 enzyme. Specifically binds polysumoylated chains via SUMO interaction motifs (SIMs) and mediates ubiquitination of sumoylated substrates. Catalyzes 'Lys-63'-linked ubiquitination of sumoylated XPC in response to UV irradiation, promoting nucleotide excision repair. Mediates ubiquitination and degradation of sumoylated PML (By similarity). The regulation of the BMP-SMAD signaling is however independent of sumoylation and is not dependent of SUMO interaction motifs (SIMs) (By similarity). PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: 3D-structure;Alternative splicing;Cytoplasm;Developmental protein;DNA damage;DNA repair;Isopeptide bond;Metal-binding;Nucleus;Reference proteome;Transferase;Ubl conjugation;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Cytoplasm Nucleus, PML body Note=Upon TGF-beta treatment, translocates from nucleus to cytosol. " Q6ZQM0,"PROTEIN NAMES: E3 ubiquitin-protein ligase rififylin (RING finger and FYVE-like domain-containing protein 1) (Fring) (RING-type E3 ubiquitin transferase rififylin) ORGANISM: Mus musculus (Mouse) FUNCTION: E3 ubiquitin-protein ligase that regulates several biological processes through the ubiquitin-mediated proteasomal degradation of various target proteins. Mediates 'Lys-48'-linked polyubiquitination of PRR5L and its subsequent proteasomal degradation thereby indirectly regulating cell migration through the mTORC2 complex. Also ubiquitinates the caspases CASP8 and CASP10, promoting their proteasomal degradation, to negatively regulate apoptosis downstream of death domain receptors. Also negatively regulates the tumor necrosis factor-mediated signaling pathway through targeting of RIPK1 to ubiquitin-mediated proteasomal degradation. Negatively regulates p53/TP53 through its direct ubiquitination and targeting to proteasomal degradation. Indirectly, may also negatively regulate p53/TP53 through ubiquitination and degradation of SFN. May also play a role in endocytic recycling. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Alternative splicing;Apoptosis;Cell membrane;Cytoplasm;Endosome;Lipoprotein;Membrane;Metal-binding;Palmitate;Phosphoprotein;Reference proteome;Repeat;Transferase;Ubl conjugation;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cytoplasm, cytosol Cell membrane ; Peripheral membrane protein Recycling endosome membrane ; Peripheral membrane protein Note=The FYVE-type zinc finger may mediate phosphatidylinositol phosphate-binding and control subcellular localization. " Q6ZS10,"PROTEIN NAMES: C-type lectin domain family 17, member A (Prolectin) ORGANISM: Homo sapiens (Human) FUNCTION: Cell surface receptor which may be involved in carbohydrate-mediated communication between cells in the germinal center. Binds glycans with terminal alpha-linked mannose or fucose residues. KEYWORDS: Alternative splicing;Calcium;Disulfide bond;Glycoprotein;Lectin;Mannose-binding;Membrane;Metal-binding;Receptor;Reference proteome;Signal-anchor;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Single-pass type II membrane protein Note=In fibroblasts, expressed on the cell surface. MISCELLANEOUS: [Isoform 2]: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay." Q6ZSY5,PROTEIN NAMES: Protein phosphatase 1 regulatory subunit 3F (R3F) ORGANISM: Homo sapiens (Human) FUNCTION: Glycogen-targeting subunit for protein phosphatase 1 (PP1). KEYWORDS: Alternative splicing;Membrane;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Single-pass membrane protein Q6ZTQ4,PROTEIN NAMES: Cadherin-related family member 3 (Cadherin-like protein 28) ORGANISM: Homo sapiens (Human) FUNCTION: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.; FUNCTION: (Microbial infection) Acts as a receptor for human rhinovirus C. KEYWORDS: 3D-structure;Alternative splicing;Asthma;Calcium;Cell adhesion;Cell membrane;Glycoprotein;Host cell receptor for virus entry;Host-virus interaction;Membrane;Receptor;Reference proteome;Repeat;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein Q6ZVN8,"PROTEIN NAMES: Hemojuvelin (Hemochromatosis type 2 protein) (Hemojuvelin BMP coreceptor) (RGM domain family member C) PROTEIN FAMILY: Repulsive guidance molecule (RGM) family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the repulsive guidance molecule (RGM) family. FUNCTION: Acts as a bone morphogenetic protein (BMP) coreceptor. Through enhancement of BMP signaling regulates hepcidin (HAMP) expression and regulates iron homeostasis. KEYWORDS: 3D-structure;Alternative splicing;Autocatalytic cleavage;Cell membrane;Disease variant;Disulfide bond;Glycoprotein;GPI-anchor;Lipoprotein;Membrane;Phosphoprotein;Reference proteome;Signal SUBCELLULAR LOCATION: Cell membrane ; Lipid-anchor, GPI-anchor Note=Also released in the extracellular space. " Q6ZWT7,PROTEIN NAMES: Lysophospholipid acyltransferase 2 (LPLAT 2) (1-acylglycerophosphate O-acyltransferase MBOAT2) (1-acylglycerophosphocholine O-acyltransferase MBOAT2) (1-acylglycerophosphoethanolamine MBOAT2 O-acyltransferase) (Lysophosphatidic acid acyltransferase) (LPAAT) (Lyso-PA acyltransferase) (Lysophosphatidylcholine acyltransferase) (LPCAT) (Lyso-PC acyltransferase) (Lysophosphatidylcholine acyltransferase 4) (Lyso-PC acyltransferase 4) (Lysophosphatidylethanolamine acyltransferase) (LPEAT) (Lyso-PE acyltransferase) (Membrane-bound O-acyltransferase domain-containing protein 2) (O-acyltransferase domain-containing protein 2) PROTEIN FAMILY: Membrane-bound acyltransferase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the membrane-bound acyltransferase family. FUNCTION: Acyltransferase which catalyzes the transfer of an acyl group from an acyl-CoA to a lysophospholipid leading to the production of a phospholipid and participates in the reacylation step of the phospholipid remodeling pathway also known as the Lands cycle. Catalyzes preferentially the acylation of lysophosphatidylethanolamine (1-acyl-sn-glycero-3-phosphoethanolamine or LPE) and lysophosphatidic acid (LPA) and to a lesser extend lysophosphatidylcholine (LPC) and lysophosphatidylserine (LPS). Prefers oleoyl-CoA as the acyl donor. May be involved in chondrocyte differentiation (By similarity). PATHWAY: Lipid metabolism; phospholipid metabolism. KEYWORDS: Acyltransferase;Endoplasmic reticulum;Lipid biosynthesis;Lipid metabolism;Membrane;Phospholipid biosynthesis;Phospholipid metabolism;Reference proteome;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Multi-pass membrane protein Endoplasmic reticulum Q700C7,"PROTEIN NAMES: Transcription factor SPEECHLESS (Basic helix-loop-helix protein 98) (AtbHLH98) (bHLH 98) (Transcription factor EN 19) (bHLH transcription factor bHLH098) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Transcription factor acting as an integration node for stomata and brassinosteroid (BR) signaling pathways to control stomatal initiation and development. Activates transcription when in the presence of SCRM/ICE1. Functions as a dimer with SCRM or SCRM2 during stomatal initiation. Required for the initiation, the spacing and the formation of stomata, by promoting the first asymmetric cell divisions. Together with FMA and MUTE, modulates the stomata formation. Involved in the regulation of growth reduction under osmotic stress (e.g. mannitol), associated with a quick decrease of meristemoid mother cells (MMCs) number lower stomatal index and density. KEYWORDS: Activator;Developmental protein;DNA-binding;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " Q700D2,"PROTEIN NAMES: Zinc finger protein JACKDAW (ID1-like zinc finger protein 3) (Protein indeterminate-domain 10) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Transcription factor that, together with BIB, regulates tissue boundaries and asymmetric cell division by a rapid up-regulation of 'SCARECROW' (SCR), thus controlling the nuclear localization of 'SHORT-ROOT' (SHR) and restricting its action. Binds DNA via its zinc fingers. Recognizes and binds to SCL3 promoter sequence 5'-AGACAA-3' to promote its expression when in complex with RGA. Confines CYCD6 expression to the cortex-endodermis initial/daughter (CEI/CEID) tissues. Required for radial patterning and stem cell maintenance. Counteracted by 'MAGPIE' (MGP). Binds to the SCR and MGP promoter sequences. Controls position-dependent signals that regulate epidermal-cell-type patterning. KEYWORDS: 3D-structure;Activator;DNA-binding;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Repeat;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: Early initiation of expression in ground tissue is SHR- and SCR-independent but later maintenance becomes dependent on both." Q70BM6,"PROTEIN NAMES: Organic anion transporter 3 (pOAT3) (Organic anion/dicarboxylate exchanger) (Solute carrier family 22 member 8) PROTEIN FAMILY: Major facilitator superfamily, Organic cation transporter family ORGANISM: Sus scrofa (Pig) SIMILARITY: Belongs to the major facilitator (TC 2.A.1) superfamily. Organic cation transporter (TC 2.A.1.19) family. FUNCTION: Functions as an organic anion/dicarboxylate exchanger that couples organic anion uptake indirectly to the sodium gradient (By similarity). Transports organic anions such as estrone 3-sulfate (E1S) and urate in exchange for dicarboxylates such as glutarate or ketoglutarate (2-oxoglutarate) (Probable). Plays an important role in the excretion of endogenous and exogenous organic anions, especially from the kidney and the brain. E1S transport is pH- and chloride-dependent and may also involve E1S/cGMP exchange (By similarity). Responsible for the transport of prostaglandin E2 (PGE2) and prostaglandin F2(alpha) (PGF2(alpha)) in the basolateral side of the renal tubule. Involved in the transport of neuroactive tryptophan metabolites kynurenate and xanthurenate. Functions as a biopterin transporters involved in the uptake and the secretion of coenzymes tetrahydrobiopterin (BH4), dihydrobiopterin (BH2) and sepiapterin to urine, thereby determining baseline levels of blood biopterins. May be involved in the basolateral transport of steviol, a metabolite of the popular sugar substitute stevioside. May participate in the detoxification/ renal excretion of drugs and xenobiotics, such as the histamine H(2)-receptor antagonists fexofenadine and cimetidine, the antibiotic benzylpenicillin (PCG), the anionic herbicide 2,4-dichloro-phenoxyacetate (2,4-D), the diagnostic agent p-aminohippurate (PAH), the antiviral acyclovir (ACV), and the mycotoxin ochratoxin (OTA), by transporting these exogenous organic anions across the cell membrane in exchange for dicarboxylates such as 2-oxoglutarate (By similarity). Contributes to the renal uptake of potent uremic toxins (indoxyl sulfate (IS), indole acetate (IA), hippurate/N-benzoylglycine (HA) and 3-carboxy-4-methyl-5-propyl-2-furanpropionate (CMPF)), pravastatin, PCG, E1S and dehydroepiandrosterone sulfate (DHEAS), and is partly involved in the renal uptake of temocaprilat (an angiotensin-converting enzyme (ACE) inhibitor) (By similarity). May contribute to the release of cortisol in the adrenals (By similarity). Involved in one of the detoxification systems on the choroid plexus (CP), removes substrates such as E1S or taurocholate (TC), PCG, 2,4-D and PAH, from the cerebrospinal fluid (CSF) to the blood for eventual excretion in urine and bile (By similarity). Also contributes to the uptake of several other organic compounds such as the prostanoids prostaglandin E(2) and prostaglandin F(2-alpha), L-carnitine, and the therapeutic drugs allopurinol, 6-mercaptopurine (6-MP) and 5-fluorouracil (5-FU) (By similarity). Mediates the transport of PAH, PCG, and the statins pravastatin and pitavastatin, from the cerebrum into the blood circulation across the blood-brain barrier (BBB). In summary, plays a role in the efflux of drugs and xenobiotics, helping reduce their undesired toxicological effects on the body (By similarity). KEYWORDS: Cell membrane;Detoxification;Glycoprotein;Ion transport;Lipid transport;Membrane;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Basolateral cell membrane ; Multi-pass membrane protein Note=Localizes on the brush border membrane of the choroid epithelial cells. Localizes to the basolateral membrane of the proximal tubular cells. Localizes on the abluminal and possibly, luminal membrane of the brain capillary endothelial cells (BCEC) (By similarity). " Q70IA6,"PROTEIN NAMES: MOB kinase activator 2 (HCCA2) (Mob2 homolog) (Mps one binder kinase activator-like 2) PROTEIN FAMILY: MOB1/phocein family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the MOB1/phocein family. FUNCTION: Stimulates the autophosphorylation and kinase activity of STK38 and STK38L. KEYWORDS: Alternative splicing;Cytoplasm;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Zinc SUBCELLULAR LOCATION: Nucleus Cytoplasm, perinuclear region " Q70KY3,PROTEIN NAMES: Laccase-1 (Benzenediol:oxygen oxidoreductase 1) (Diphenol oxidase 1) (Ligninolytic phenoloxidase) (Urishiol oxidase 1) PROTEIN FAMILY: Multicopper oxidase family ORGANISM: Melanocarpus albomyces SIMILARITY: Belongs to the multicopper oxidase family. FUNCTION: Lignin degradation and detoxification of lignin-derived products. KEYWORDS: 3D-structure;Copper;Direct protein sequencing;Disulfide bond;Glycoprotein;Lignin degradation;Metal-binding;Oxidoreductase;Signal Q70US9,"PROTEIN NAMES: Volkensin (allergen Ade v RIP) [Cleaved into: Volkensin A chain (VKA) (Ribosome-inactivating protein volkensin) (RIP VK) (rRNA N-glycosidase); Volkensin B chain (VKB)] PROTEIN FAMILY: Ribosome-inactivating protein family, Type 2 RIP subfamily ORGANISM: Adenia volkensii (Kilyambiti plant) SIMILARITY: In the N-terminal section; belongs to the ribosome-inactivating protein family. Type 2 RIP subfamily. FUNCTION: [Volkensin A chain]: Has N-glycosidase activity and is responsible for inhibiting protein synthesis through the catalytic inactivation of 60S ribosomal subunits by removing a specific adenine of 28S rRNA. Inhibits GTP-dependent binding of EF2 (elongation factor 2) to ribosomes.; FUNCTION: [Volkensin B chain]: Binds to cell receptors and probably facilitates the entry into the cell of the A chain (By similarity). Acts also as a galactose-specific lectin responsible for cell agglutination. KEYWORDS: Allergen;Direct protein sequencing;Disulfide bond;Glycoprotein;Glycosidase;Hydrolase;Lectin;Plant defense;Protein synthesis inhibitor;Toxin " Q70UZ7,PROTEIN NAMES: von Willebrand factor A domain-containing protein 2 (A domain-containing protein similar to matrilin and collagen) (AMACO) ORGANISM: Mus musculus (Mouse) KEYWORDS: Alternative splicing;Disulfide bond;EGF-like domain;Glycoprotein;Reference proteome;Repeat;Secreted;Signal SUBCELLULAR LOCATION: Secreted. Q719N1,"PROTEIN NAMES: Spastin PROTEIN FAMILY: AAA ATPase family, Spastin subfamily ORGANISM: Sus scrofa (Pig) SIMILARITY: Belongs to the AAA ATPase family. Spastin subfamily. FUNCTION: ATP-dependent microtubule severing protein that specifically recognizes and cuts microtubules that are polyglutamylated. Preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold. Severing activity is not dependent on tubulin acetylation or detyrosination. Microtubule severing promotes reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. It is critical for the biogenesis and maintenance of complex microtubule arrays in axons, spindles and cilia. SPAST is involved in abscission step of cytokinesis and nuclear envelope reassembly during anaphase in cooperation with the ESCRT-III complex. Recruited at the midbody, probably by IST1, and participates in membrane fission during abscission together with the ESCRT-III complex. Recruited to the nuclear membrane by IST1 and mediates microtubule severing, promoting nuclear envelope sealing and mitotic spindle disassembly during late anaphase. Required for membrane traffic from the endoplasmic reticulum (ER) to the Golgi and endosome recycling. Recruited by IST1 to endosomes and regulates early endosomal tubulation and recycling by mediating microtubule severing. Probably plays a role in axon growth and the formation of axonal branches. KEYWORDS: Allosteric enzyme;ATP-binding;Cell cycle;Cell division;Cell projection;Cytoplasm;Cytoskeleton;Developmental protein;Differentiation;Endoplasmic reticulum;Isomerase;Membrane;Microtubule;Neurogenesis;Nucleotide-binding;Nucleus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Membrane ; Peripheral membrane protein Endoplasmic reticulum Midbody Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Cytoplasm, cytoskeleton Cytoplasm, perinuclear region Nucleus Cytoplasm, cytoskeleton, spindle Cytoplasm Cell projection, axon Note=Forms an intramembrane hairpin-like structure in the membrane. Localization to the centrosome is independent of microtubules. Localizes to the midbody of dividing cells, and this requires CHMP1B. Enriched in the distal axons and branches of postmitotic neurons. Localizes to endoplasmic reticulum tubular network. Mainly nuclear in interphase cells and becomes associated with the centrosomes, spindle microtubules, midzone and finally the midbody during cell division (By similarity). " Q71DJ5,"PROTEIN NAMES: Triacylglycerol lipase 1 PROTEIN FAMILY: AB hydrolase superfamily, Lipase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the AB hydrolase superfamily. Lipase family. FUNCTION: Triacylglycerol (TAG) lipase active on triolein, trioctanoin, tributyrin and 1,3-Diolein, but not on phospho- and galactolipids. Involved but dispensable for TAG storage breakdown during seed germination. PATHWAY: Lipid metabolism; glycerolipid metabolism. KEYWORDS: Direct protein sequencing;Gibberellin signaling pathway;Glycoprotein;Hydrolase;Lipid degradation;Lipid metabolism;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted " Q71EW5,"PROTEIN NAMES: Acetylene hydratase (Ahy) PROTEIN FAMILY: Prokaryotic molybdopterin-containing oxidoreductase family ORGANISM: Syntrophotalea acetylenica (Pelobacter acetylenicus) SIMILARITY: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. FUNCTION: Catalyzes the hydration of acetylene to form acetaldehyde. Ethylene cannot act as a substrate. KEYWORDS: 3D-structure;4Fe-4S;Direct protein sequencing;Iron;Iron-sulfur;Lyase;Metal-binding;Tungsten MISCELLANEOUS: The tungsten center binds a water molecule that is activated by Asp-13 residue, enabling it to attack acetylene bound in a distinct hydrophobic pocket." Q71F23,"PROTEIN NAMES: Centromere protein U (CENP-U) (Centromere protein of 50 kDa) (CENP-50) (Interphase centromere complex protein 24) (KSHV latent nuclear antigen-interacting protein 1) (MLF1-interacting protein) (Polo-box-interacting protein 1) PROTEIN FAMILY: CENP-U/AME1 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the CENP-U/AME1 family. FUNCTION: Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Plays an important role in the correct PLK1 localization to the mitotic kinetochores. A scaffold protein responsible for the initial recruitment and maintenance of the kinetochore PLK1 population until its degradation. Involved in transcriptional repression. KEYWORDS: 3D-structure;Alternative splicing;Centromere;Chromosome;Coiled coil;Cytoplasm;Host-virus interaction;Isopeptide bond;Kinetochore;Nucleus;Phosphoprotein;Reference proteome;Repressor;Transcription;Transcription regulation;Ubl conjugation SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Chromosome, centromere, kinetochore. Note=Localizes in the kinetochore domain of centromeres. Colocalizes with PLK1 at the interzone between the inner and the outer kinetochore plates. " Q72EF3,"PROTEIN NAMES: Cytochrome c nitrite reductase subunit NrfA (cNiR subunit NrfA) (Cytochrome c-type protein NrfA) PROTEIN FAMILY: Cytochrome c-552 family ORGANISM: Nitratidesulfovibrio vulgaris (strain ATCC 29579 / DSM 644 / CCUG 34227 / NCIMB 8303 / VKM B-1760 / Hildenborough) (Desulfovibrio vulgaris) SIMILARITY: Belongs to the cytochrome c-552 family. FUNCTION: Catalytic subunit of the cytochrome c nitrite reductase holocomplex NrfHA. Has both nitrite and sulfite reductase activities. Catalyzes the reduction of nitrite to ammonia, consuming six electrons acquired by the electron donor subunit NrfH from the menaquinone pool, in an anaerobic respiratory process of nitrite. The other biological function of the NrfHA holocomplex is to detoxify nitrite. This function is essential for the survival of this organism as it enables it to overcome inhibition by nitrite, which is produced by other organisms living in the same environment (Probable). KEYWORDS: 3D-structure;Calcium;Cell inner membrane;Cell membrane;Heme;Iron;Membrane;Metal-binding;Oxidoreductase;Reference proteome;Signal;Stress response SUBCELLULAR LOCATION: Cell inner membrane ; Peripheral membrane protein ; Periplasmic side " Q72EF4,"PROTEIN NAMES: Cytochrome c nitrite reductase subunit NrfH (cNiR subunit NrfH) (Cytochrome c quinol dehydrogenase NrfH) (Cytochrome c-type protein NrfH) PROTEIN FAMILY: NapC/NirT/NrfH family ORGANISM: Nitratidesulfovibrio vulgaris (strain ATCC 29579 / DSM 644 / CCUG 34227 / NCIMB 8303 / VKM B-1760 / Hildenborough) (Desulfovibrio vulgaris) SIMILARITY: Belongs to the NapC/NirT/NrfH family. FUNCTION: Electron donor subunit of the cytochrome c nitrite reductase holocomplex NrfHA. Acquires electrons from the menaquinone pool and mediates their transfer to the catalytic subunit NrfA in an anaerobic respiratory process of nitrite. The other biological function of the NrfHA holocomplex is to detoxify nitrite. This function is essential for the survival of this organism as it enables it to overcome inhibition by nitrite, which is produced by other organisms living in the same environment (Probable). KEYWORDS: 3D-structure;Cell inner membrane;Cell membrane;Direct protein sequencing;Electron transport;Heme;Iron;Membrane;Metal-binding;Reference proteome;Signal-anchor;Stress response;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell inner membrane ; Single-pass type II membrane protein " Q72IB8,PROTEIN NAMES: Proline dehydrogenase (PRODH) (Proline oxidase) (TtPRODH) PROTEIN FAMILY: Proline dehydrogenase family ORGANISM: Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27) SIMILARITY: Belongs to the proline dehydrogenase family. FUNCTION: Converts proline to delta-1-pyrroline-5-carboxylate. Has significant activity against O(2) producing superoxide during proline oxidation catalytic cycle. PATHWAY: Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 1/2. KEYWORDS: 3D-structure;FAD;Flavoprotein;Nucleotide-binding;Oxidoreductase;Proline metabolism Q72LF3,"PROTEIN NAMES: tRNA-5-methyluridine(54) 2-sulfurtransferase (2-thiouridine synthetase TtuA) (tRNA two-thiouridine-synthesizing protein A) PROTEIN FAMILY: TtcA family, TtuA subfamily ORGANISM: Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27) SIMILARITY: Belongs to the TtcA family. TtuA subfamily. FUNCTION: Catalyzes the ATP-dependent 2-thiolation of 5-methyluridine residue at position 54 in the T loop of tRNAs, leading to 5-methyl-2-thiouridine (m(5)s(2)U or s(2)T). This modification allows thermal stabilization of tRNAs in thermophilic microorganisms, and is required for cell growth at high temperatures. TtuA transfers the S atom from the thiocarboxylated C-terminus of TtuB to tRNA. PATHWAY: tRNA modification. KEYWORDS: 3D-structure;4Fe-4S;ATP-binding;Iron;Iron-sulfur;Isopeptide bond;Magnesium;Metal-binding;Nucleotide-binding;RNA-binding;Transferase;tRNA processing;tRNA-binding;Ubl conjugation;Zinc MISCELLANEOUS: The thiolation reaction consists of two steps: a first activation step by ATP to form an adenylated intermediate of the target base of tRNA, and a second nucleophilic substitution step of the sulfur (S) atom supplied by thiocarboxylated TtuB for the adenyl group. However, it is not clear if the S-transfer mechanism is direct or indirect." Q74K42,"PROTEIN NAMES: Inulosucrase (IS) PROTEIN FAMILY: Glycosyl hydrolase 68 family ORGANISM: Lactobacillus johnsonii (strain CNCM I-12250 / La1 / NCC 533) SIMILARITY: Belongs to the glycosyl hydrolase 68 family. FUNCTION: Fructosyltransferase that catalyzes the polymerization of the fructose moiety of sucrose to produce inulin polymer and inulin oligosaccharides such as 1-kestose and nystose. KEYWORDS: 3D-structure;Calcium;Carbohydrate metabolism;Cell wall;Glycosyltransferase;Metal-binding;Peptidoglycan-anchor;Secreted;Signal;Transferase SUBCELLULAR LOCATION: Secreted, cell wall ; Peptidoglycan-anchor " Q752Z9,"PROTEIN NAMES: Inosine triphosphate pyrophosphatase (ITPase) (Inosine triphosphatase) (Non-canonical purine NTP pyrophosphatase) (Non-standard purine NTP pyrophosphatase) (Nucleoside-triphosphate diphosphatase) (Nucleoside-triphosphate pyrophosphatase) (NTPase) PROTEIN FAMILY: HAM1 NTPase family ORGANISM: Eremothecium gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Ashbya gossypii) SIMILARITY: Belongs to the HAM1 NTPase family. FUNCTION: Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as inosine triphosphate (ITP), deoxyinosine triphosphate (dITP) or xanthosine 5'-triphosphate (XTP) to their respective monophosphate derivatives. The enzyme does not distinguish between the deoxy- and ribose forms. Probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions. KEYWORDS: Cytoplasm;Hydrolase;Magnesium;Manganese;Metal-binding;Nucleotide metabolism;Nucleotide-binding;Nucleus;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Nucleus " Q75HE5,PROTEIN NAMES: NAC domain-containing protein 2 (ONAC002) (OsNAC19) (Protein STRESS-RESPONSIVE NAC 1) ORGANISM: Oryza sativa subsp. japonica (Rice) FUNCTION: Transcription factor that possesses transactivation activity. Transcription activator involved in response to abiotic stresses. Plays a positive role during dehydration and salt stress. Binds specifically to the 5'-CATGTG-3' motif found in promoters of stress-responsive genes. KEYWORDS: 3D-structure;Activator;DNA-binding;Nucleus;Reference proteome;Stress response;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: Plants overexpressing NAC002 exhibit improved tolerance to drought and salt stresses. Q75JI3,"PROTEIN NAMES: Vesicle-fusing ATPase (N-ethylmaleimide-sensitive factor A) (NEM-sensitive fusion protein A) (Vesicular-fusion protein nfsA) PROTEIN FAMILY: AAA ATPase family ORGANISM: Dictyostelium discoideum (Social amoeba) SIMILARITY: Belongs to the AAA ATPase family. FUNCTION: Required for vesicle-mediated transport. Involved in endocytosis and endosome-endosome fusion. May be required for transport from the endoplasmic reticulum to the Golgi stack, and for the fusion of transport vesicles within the Golgi cisternae. Required for cell polarity, locomotion and chemotaxis. KEYWORDS: ATP-binding;Chemotaxis;Cytoplasmic vesicle;Endosome;ER-Golgi transport;Hydrolase;Magnesium;Membrane;Metal-binding;Nucleotide-binding;Protein transport;Reference proteome;Repeat;Transport SUBCELLULAR LOCATION: Cytoplasmic vesicle membrane ; Peripheral membrane protein ; Cytoplasmic side Endosome membrane ; Peripheral membrane protein ; Cytoplasmic side " Q75N35,"PROTEIN NAMES: Dioscorin DB3L (Tuber storage protein DB3L) PROTEIN FAMILY: Alpha-class carbonic anhydrase family ORGANISM: Dioscorea polystachya (Chinese yam) SIMILARITY: Belongs to the alpha-class carbonic anhydrase family. FUNCTION: Maltose-binding lectin. No affinity is detected toward glucose. Has hemagglutinating activity against rabbit erythrocytes at 3.9 ug/ml. No carbonate dehydratase or trypsin inhibitor activity detected by measuring the hydrolysis of 4-nitrophenyl acetate or the inhibition of bovine trypsin-catalyzed hydrolysis of N-benzoyl-L-arginine ethyl ester, respectively. KEYWORDS: Direct protein sequencing;Disulfide bond;Lectin;Signal " Q76FS5,"PROTEIN NAMES: Solanesyl diphosphate synthase 2, chloroplastic (AtSPS2) (All-trans-nonaprenyl-diphosphate synthase 2 (geranylgeranyl-diphosphate specific)) PROTEIN FAMILY: FPP/GGPP synthase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the FPP/GGPP synthase family. FUNCTION: Involved in providing solanesyl diphosphate for plastoquinone-9 (PQ-9) formation in plastids (Probable). Catalyzes the elongation of the prenyl side chain of PQ-9 in plastids. Contributes to the biosynthesis of plastochromanol-8 (PC-8) in plastids. Does not contribute to the synthesis of tocopherol or ubiquinone. PQ-9 and PC-8 are lipophilic antioxidants that act as protectant against photooxidative stress under high light stress conditions. Prefers geranylgeranyl diphosphate to farnesyl diphosphate as substrate. No activity with geranyl diphosphate or dimethylallyl diphosphate as substrate. KEYWORDS: Chloroplast;Isoprene biosynthesis;Magnesium;Metal-binding;Plastid;Reference proteome;Stress response;Transferase;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast " Q76HL1,"PROTEIN NAMES: Serine protease 43 PROTEIN FAMILY: Peptidase S1 family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the peptidase S1 family. FUNCTION: Plays a role in spermatogenesis. Involved in germ cell survival during meiosis. Lacks protease activity in vitro. KEYWORDS: Cell membrane;Differentiation;Disulfide bond;Glycoprotein;GPI-anchor;Hydrolase;Lipoprotein;Membrane;Protease;Reference proteome;Signal;Spermatogenesis;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Lipid-anchor, GPI-anchor " Q76L36,"PROTEIN NAMES: NADPH-dependent conjugated polyketone reductase C2 (CPR-C2) (2-dehydropantolactone reductase) (Ketopantoyl-lactone reductase) PROTEIN FAMILY: Aldo/keto reductase family ORGANISM: Candida parapsilosis (Yeast) SIMILARITY: Belongs to the aldo/keto reductase family. FUNCTION: NADPH-dependent conjugated polyketone reductase with broad substrate specificity and strict stereospecificity. Reduces ketopantoyl lactone (2-dehydropantolactone), isatin and camphorquinone. Does not act on menadione, p-nitrobenzaldehyde and pyridine-3-aldehyde. KEYWORDS: 3D-structure;Direct protein sequencing;NADP;Oxidoreductase " Q78T81,"PROTEIN NAMES: Early estrogen-induced gene 1 protein (EEIG1) PROTEIN FAMILY: EEIG family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the EEIG family. FUNCTION: Key component of TNFSF11/RANKL- and TNF-induced osteoclastogenesis pathways, thereby mediates bone resorption in pathological bone loss conditions. Required for TNFSF11/RANKL-induced osteoclastogenesis via its interaction with TNFRSF11A/RANK, thereby facilitates the downsteam transcription of NFATC1 and activation of PLCG2. Facilitates recruitment of the transcriptional repressor PRDM1/BLIMP1 to the promoter of the anti-osteoclastogenesis gene IRF8, thereby resulting in transcription of osteoclast differentiation factors. May play a role in estrogen action (By similarity). KEYWORDS: Cytoplasm;Membrane;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus Cytoplasm Membrane raft " Q79FV4,"PROTEIN NAMES: S-sulfocysteine synthase (O-phospho-L-serine-dependent S-sulfocysteine synthase) (OPS-dependent S-sulfocysteine synthase) (O-phosphoserine sulfhydrylase) PROTEIN FAMILY: Cysteine synthase/cystathionine beta-synthase family ORGANISM: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) SIMILARITY: Belongs to the cysteine synthase/cystathionine beta-synthase family. FUNCTION: Catalyzes the synthesis of S-sulfocysteine, utilizing O-phosphoserine (OPS) and thiosulfate as substrates. To a lesser extent, can also use sulfide as donor substrate, producing L-cysteine. CysK2 thus provides a third metabolic route to cysteine, either directly using sulfide as donor or indirectly via S-sulfocysteine. S-sulfocysteine might also act as a signaling molecule triggering additional responses in redox defense in the pathogen upon exposure to reactive oxygen species during intracellular survival or dormancy. Cannot utilize thiocarboxylated CysO as a sulfur donor and O-acetylserine (OAS) as acceptor substrate. PATHWAY: Amino-acid biosynthesis; L-cysteine biosynthesis. KEYWORDS: Amino-acid biosynthesis;Cysteine biosynthesis;Pyridoxal phosphate;Reference proteome;Transferase MISCELLANEOUS: Was identified as a high-confidence drug target.; MISCELLANEOUS: The enzyme uses a mechanism via a central aminoacrylate intermediate that is similar to that of other members of this pyridoxal phosphate-dependent enzyme family." Q79FW5,"PROTEIN NAMES: PE-PGRS family protein PE_PGRS11 (PE-PGRS phosphoglycerate mutase) PROTEIN FAMILY: Mycobacterial PE family, PGRS subfamily; Phosphoglycerate mutase family ORGANISM: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) SIMILARITY: In the N-terminal section; belongs to the mycobacterial PE family. PGRS subfamily.; SIMILARITY: In the C-terminal section; belongs to the phosphoglycerate mutase family. FUNCTION: Induces maturation and activation of human dendritic cells (DCs), via TLR2-dependent activation of ERK1/2, p38 MAPK, and NF-kappa-B signaling pathways, and enhances the ability of DCs to stimulate CD4(+) T cells. By activating DCs, could potentially contribute to the initiation of innate immune responses during tuberculosis infection and hence regulate the clinical course of tuberculosis. Involved in resistance to oxidative stress, via TLR2-dependent activation of the PI3K-ERK1/2-NF-kappa-B signaling pathway and expression of COX-2 and Bcl2. Also abolishes H(2)O(2)-triggered activation of p38 MAPK. KEYWORDS: Cell wall;Isomerase;Magnesium;Reference proteome;Secreted;Stress response;Virulence SUBCELLULAR LOCATION: Secreted, cell wall Cell surface MISCELLANEOUS: Could be an immunodominant antigen." Q7F8T6,"PROTEIN NAMES: Tricin synthase 2 (Caffeoyl-CoA 3-O-methyltransferase ROMT17) PROTEIN FAMILY: Class I-like SAM-binding methyltransferase superfamily, Cation-dependent O-methyltransferase family, CCoAMT subfamily ORGANISM: Oryza sativa subsp. japonica (Rice) SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-dependent O-methyltransferase family. CCoAMT subfamily. FUNCTION: Catalyzes the stepwise methylation of tricetin to its 3'-mono- and 3',5'-dimethyl ethers. No 3',4',5'-trimethylated ester derivatives are produced. Can use caffeoyl CoA, 5-hydroxyferulic acid, luteolin, tricetin, quercetin, myrcetin and 7,8-dihydroxyflavone as substrates, but not naringenin, apigenin or kaempferol. The 2,3-double bond and the O-dihydroxyl group of the substrate are both required for catalytic activity of the enzyme. KEYWORDS: Alternative splicing;Magnesium;Metal-binding;Methyltransferase;Reference proteome;S-adenosyl-L-methionine;Transferase " Q7FZF1,PROTEIN NAMES: Calcineurin B-like protein 5 (SOS3-like calcium-binding protein 4) PROTEIN FAMILY: Calcineurin regulatory subunit family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the calcineurin regulatory subunit family. FUNCTION: Acts as a calcium sensor. CBL proteins interact with CIPK serine-threonine protein kinases. Binding of a CBL protein to the regulatory NAF domain of a CIPK protein lead to the activation of the kinase in a calcium-dependent manner. May function as a positive regulator of salt or drought responses. KEYWORDS: Cytoplasm;Lipoprotein;Myristate;Nucleus;Reference proteome;Repeat SUBCELLULAR LOCATION: Cytoplasm Nucleus Note=Targeted to the cell membrane when interacting with CIPK24. Q7JQD3,"PROTEIN NAMES: Gelsolin-like protein 1 (Actin-modulator) (EWAM) (EWAM-P1) PROTEIN FAMILY: Villin/gelsolin family ORGANISM: Lumbricus terrestris (Common earthworm) SIMILARITY: Belongs to the villin/gelsolin family. FUNCTION: Calcium-regulated protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). Can promote the assembly of monomers into filaments (nucleation) as well as sever existing filaments. KEYWORDS: Actin capping;Actin-binding;Calcium;Cytoplasm;Cytoskeleton;Developmental protein;Repeat SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton " Q7JUX9,"PROTEIN NAMES: Mitochondrial ribonuclease P protein 1 homolog (Mitochondrial RNase P protein 1) (Protein roswell) (RNA (guanine-9-)-methyltransferase domain-containing protein 1) (tRNA methyltransferase 10 homolog C) PROTEIN FAMILY: Class IV-like SAM-binding methyltransferase superfamily, TRM10 family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the class IV-like SAM-binding methyltransferase superfamily. TRM10 family. FUNCTION: Mitochondrial tRNA N1-methyltransferase involved in mitochondrial tRNA maturation. Component of mitochondrial ribonuclease P, a complex composed of rswl/MRPP1, scu/MRPP2 and mldr/MRPP3., which cleaves tRNA molecules in their 5'-ends. Essential for the structural and functional integrity of mitochondria. Function is essential for pupal development. KEYWORDS: Coiled coil;Methyltransferase;Mitochondrion;Phosphoprotein;Reference proteome;S-adenosyl-L-methionine;Transferase;Transit peptide;tRNA processing SUBCELLULAR LOCATION: Mitochondrion " Q7JYX0,"PROTEIN NAMES: Glutathione S-transferase E14 (Protein noppera-bo) PROTEIN FAMILY: GST superfamily, Epsilon family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the GST superfamily. Epsilon family. FUNCTION: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Essential for ecdysteroid biosynthesis. May be involved in detoxification. KEYWORDS: 3D-structure;Detoxification;Lipid biosynthesis;Lipid metabolism;Reference proteome;Steroid biosynthesis;Transferase MISCELLANEOUS: Member of the Halloween gene group." Q7K1U0,"PROTEIN NAMES: Activity-regulated cytoskeleton associated protein 1 (dArc1) PROTEIN FAMILY: ARC/ARG3.1 family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the ARC/ARG3.1 family. FUNCTION: Master regulator of synaptic plasticity that self-assembles into virion-like capsids that encapsulate RNAs and mediate intercellular RNA transfer from motorneurons to muscles. Arc1 protein is released from motorneurons in extracellular vesicles that mediate the transfer of Arc1 mRNA into muscle cells, where Arc1 mRNA can undergo activity-dependent translation. Intercellular transfer od Arc1 mRNA is required for synaptic plasticity at the neuromuscular junction. May play a role in energy balance: required for regulation of body fat by a specific population of brain neurons, named E347, that are necessary and sufficient for proper body fat storage. KEYWORDS: 3D-structure;Membrane;Reference proteome;RNA-binding;Synapse;Transport SUBCELLULAR LOCATION: Extracellular vesicle membrane Synapse Note=Forms virion-like extracellular vesicles that are released from neurons. Present at pre- and postsynaptic sites of the neuromuscular junction. " Q7K3L1,"PROTEIN NAMES: Guanylate kinase-associated protein mars PROTEIN FAMILY: SAPAP family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the SAPAP family. FUNCTION: Cell cycle regulator. KEYWORDS: Cell cycle;Cell membrane;Cytoplasm;Cytoskeleton;Membrane;Nucleus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cell membrane ; Peripheral membrane protein Nucleus, nucleoplasm Cytoplasm Cytoplasm, cytoskeleton, spindle Note=Localizes to the cell membrane of epithelial cells. Its subcellular localization dynamically changes during cell-cycle progression. At the prophase, it concentrates at the nucleoplasm. In the metaphase cells, it becomes diffusive in the cytoplasm and some is concentrated at the mitotic spindles and spindle poles. " Q7K5M0,"PROTEIN NAMES: Inactive serine protease scarface PROTEIN FAMILY: Peptidase S1 family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the peptidase S1 family. FUNCTION: Inactive serine protease that plays a role in germ-band retraction and dorsal closure morphogenesis in embryogenesis; contributes to amnioserosa attachment and epithelial apico-basal polarity by regulating the localization of laminin LanA on the apical side of the amnioserosa epithelium. Contributes to epithelial morphogenesis probably by regulating the bsk/JNK pathway, as part of a negative-feedback loop, and by modulating the cross-talk between the Egfr, bsk/JNK and dpp signal transduction pathways. In larval development, antagonizes the morphogenetic movements controlled by the bsk/JNK signaling including male genitalia formation and thorax development. KEYWORDS: Developmental protein;Disulfide bond;Reference proteome;Secreted;Serine protease homolog;Signal SUBCELLULAR LOCATION: Secreted " Q7KMJ6,"PROTEIN NAMES: RNA-binding protein spenito PROTEIN FAMILY: RRM Spen family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the RRM Spen family. FUNCTION: RNA-binding protein that acts as an associated component of the WMM complex, a complex that mediates N6-methyladenosine (m6A) methylation of mRNAs. M6a modification plays a role in the efficiency of mRNA splicing and is required for sex determination. In the WMM complex, may act by binding target RNAs and recruiting the WMM complex. Required for sex determination and dosage compensation via Sxl alternative splicing: m6A methylation acts as a key regulator of Sxl pre-mRNA and promotes female-specific alternative splicing of Sxl, which determines female physiognomy. M6A methylation is also required for neuronal functions. Acts as a positive regulator of canonical Wg signaling during wing disk and eye development. KEYWORDS: Developmental protein;Differentiation;mRNA processing;mRNA splicing;Nucleus;Phosphoprotein;Reference proteome;RNA-binding;Sexual differentiation SUBCELLULAR LOCATION: Nucleus " Q7KPA5,"PROTEIN NAMES: Meiotic recombination protein W68 (SPO11 protein homolog) PROTEIN FAMILY: TOP6A family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the TOP6A family. FUNCTION: Required for meiotic recombination. Together with mei-P22, mediates DNA cleavage that forms the double-strand breaks (DSB) that initiate meiotic recombination (By similarity). KEYWORDS: DNA-binding;Isomerase;Magnesium;Meiosis;Metal-binding;Nucleus;Reference proteome;Topoisomerase SUBCELLULAR LOCATION: Nucleus " Q7KVW5,"PROTEIN NAMES: Small conductance calcium-activated potassium channel protein (Protein SK) (dSK) PROTEIN FAMILY: Potassium channel KCNN family, SK subfamily ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the potassium channel KCNN family. SK subfamily. FUNCTION: Forms a voltage-independent potassium channel activated by intracellular calcium. Activation is followed by membrane hyperpolarization. Thought to regulate neuronal excitability by contributing to the slow component of synaptic afterhyperpolarization. The channel is blocked by apamin (By similarity). KEYWORDS: Alternative splicing;Calmodulin-binding;Ion channel;Ion transport;Membrane;Reference proteome;RNA editing;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Membrane ; Multi-pass membrane protein " Q7KVY7,PROTEIN NAMES: Syntaxin-4 PROTEIN FAMILY: Syntaxin family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the syntaxin family. FUNCTION: Potentially involved in docking of synaptic vesicles at presynaptic active zones. KEYWORDS: Coiled coil;Membrane;Neurotransmitter transport;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Membrane ; Single-pass type IV membrane protein Q7L190,PROTEIN NAMES: Developmental pluripotency-associated protein 4 ORGANISM: Homo sapiens (Human) FUNCTION: May be involved in the maintenance of active epigenetic status of target genes. May inhibit differentiation of embryonic cells into a primitive ectoderm lineage. KEYWORDS: Developmental protein;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus Note=Associated with transcriptionally active chromatin. Q7L273,"PROTEIN NAMES: BTB/POZ domain-containing protein KCTD9 ORGANISM: Homo sapiens (Human) FUNCTION: Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex, which mediates the ubiquitination of target proteins, leading to their degradation by the proteasome. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: 3D-structure;Phosphoprotein;Reference proteome;Repeat;Ubl conjugation pathway " Q7L4P6,PROTEIN NAMES: BEN domain-containing protein 5 ORGANISM: Homo sapiens (Human) FUNCTION: Acts as a transcriptional repressor. KEYWORDS: Acetylation;Alternative splicing;Coiled coil;DNA-binding;Isopeptide bond;Reference proteome;Repressor;Transcription;Transcription regulation;Ubl conjugation Q7L775,PROTEIN NAMES: EPM2A-interacting protein 1 (Laforin-interacting protein) ORGANISM: Homo sapiens (Human) KEYWORDS: Endoplasmic reticulum;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Endoplasmic reticulum Q7LFX5,"PROTEIN NAMES: Carbohydrate sulfotransferase 15 (B-cell RAG-associated gene protein) (hBRAG) (N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase) (GalNAc4S-6ST) PROTEIN FAMILY: Sulfotransferase 1 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the sulfotransferase 1 family. FUNCTION: Sulfotransferase that transfers sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to the C-6 hydroxyl group of the GalNAc 4-sulfate residue of chondroitin sulfate A and forms chondroitin sulfate E containing GlcA-GalNAc(4,6-SO(4)) repeating units. It also transfers sulfate to a unique non-reducing terminal sequence, GalNAc(4SO4)-GlcA(2SO4)-GalNAc(6SO4), to yield a highly sulfated structure similar to the structure found in thrombomodulin chondroitin sulfate. May also act as a B-cell receptor involved in BCR ligation-mediated early activation that mediate regulatory signals key to B-cell development and/or regulation of B-cell-specific RAG expression; however such results are unclear in vivo. KEYWORDS: Alternative splicing;Disulfide bond;Glycoprotein;Golgi apparatus;Membrane;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus membrane ; Single-pass type II membrane protein Note=A small fraction may also be present at the cell surface, where it acts as a B-cell receptor. " Q7LGC8,"PROTEIN NAMES: Carbohydrate sulfotransferase 3 (Chondroitin 6-O-sulfotransferase 1) (C6ST-1) (Chondroitin 6-sulfotransferase) (C6ST) (Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 0) (GST-0) PROTEIN FAMILY: Sulfotransferase 1 family, Gal/GlcNAc/GalNAc subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the sulfotransferase 1 family. Gal/GlcNAc/GalNAc subfamily. FUNCTION: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of the N-acetylgalactosamine (GalNAc) residue of chondroitin. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage and is distributed on the surfaces of many cells and extracellular matrices. Catalyzes with a lower efficiency the sulfation of Gal residues of keratan sulfate, another glycosaminoglycan. Can also catalyze the sulfation of the Gal residues in sialyl N-acetyllactosamine (sialyl LacNAc) oligosaccharides (By similarity). May play a role in the maintenance of naive T-lymphocytes in the spleen (By similarity). KEYWORDS: Carbohydrate metabolism;Disease variant;Glycoprotein;Golgi apparatus;Membrane;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus membrane ; Single-pass type II membrane protein " Q7M418,PROTEIN NAMES: Extracellular giant hemoglobin major globin subunit B2 (Major globin chain c) PROTEIN FAMILY: Globin family ORGANISM: Oligobrachia mashikoi (Beard worm) SIMILARITY: Belongs to the globin family. FUNCTION: The extracellular giant hemoglobin is able to bind and transport oxygen and hydrosulfide simultaneously and reversibly at two different sites. KEYWORDS: 3D-structure;Direct protein sequencing;Disulfide bond;Heme;Iron;Metal-binding;Oxygen transport;Secreted;Signal;Transport SUBCELLULAR LOCATION: Secreted. Q7M6Y3,"PROTEIN NAMES: Phosphatidylinositol-binding clathrin assembly protein (Clathrin assembly lymphoid myeloid leukemia) (CALM) PROTEIN FAMILY: PICALM/SNAP91 family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the PICALM/SNAP91 family. FUNCTION: Cytoplasmic adapter protein that plays a critical role in clathrin-mediated endocytosis which is important in processes such as internalization of cell receptors, synaptic transmission or removal of apoptotic cells. Recruits AP-2 and attaches clathrin triskelions to the cytoplasmic side of plasma membrane leading to clathrin-coated vesicles (CCVs) assembly. Furthermore, regulates clathrin-coated vesicle size and maturation by directly sensing and driving membrane curvature. In addition to binding to clathrin, mediates the endocytosis of small R-SNARES (Soluble NSF Attachment Protein REceptors) between plasma membranes and endosomes including VAMP2, VAMP3, VAMP4, VAMP7 or VAMP8. In turn, PICALM-dependent SNARE endocytosis is required for the formation and maturation of autophagic precursors. Modulates thereby autophagy and the turnover of autophagy substrates such as MAPT/TAU or amyloid precursor protein cleaved C-terminal fragment (APP-CTF). KEYWORDS: Acetylation;Alternative splicing;Cell membrane;Coated pit;Cytoplasmic vesicle;Developmental protein;Endocytosis;Golgi apparatus;Isopeptide bond;Membrane;Nucleus;Phosphoprotein;Reference proteome;Ubl conjugation SUBCELLULAR LOCATION: Cell membrane Membrane, clathrin-coated pit Golgi apparatus Cytoplasmic vesicle, clathrin-coated vesicle Nucleus Note=Colocalized with clathrin in the Golgi area. Interaction with PIMREG may target PICALM to the nucleus in some cells. " Q7MGG3,"PROTEIN NAMES: Riboflavin biosynthesis protein VVA0006 [Includes: Riboflavin biosynthesis intermediates N-glycosidase ; GTP cyclohydrolase-2 (GTP cyclohydrolase II)] PROTEIN FAMILY: YbiA family; GTP cyclohydrolase II family ORGANISM: Vibrio vulnificus (strain YJ016) SIMILARITY: In the N-terminal section; belongs to the YbiA family.; SIMILARITY: In the C-terminal section; belongs to the GTP cyclohydrolase II family. FUNCTION: Catalyzes the hydrolysis of the N-glycosidic bond in the first two intermediates of riboflavin biosynthesis, which are highly reactive metabolites, yielding relatively innocuous products. Thus, can divert a surplus of harmful intermediates into relatively harmless products and pre-empt the damage these intermediates would otherwise do. Has no activity against GTP, nucleoside monophosphates or ADP-ribose.; FUNCTION: Catalyzes the conversion of GTP to 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate. PATHWAY: Cofactor biosynthesis; riboflavin biosynthesis; 5-amino-6-(D-ribitylamino)uracil from GTP: step 1/4. KEYWORDS: Glycosidase;GTP-binding;Hydrolase;Metal-binding;Multifunctional enzyme;Nucleotide-binding;Riboflavin biosynthesis;Zinc " Q7NDN8,"PROTEIN NAMES: Proton-gated ion channel (GLIC) (Ligand-gated ion channel) (LGIC) PROTEIN FAMILY: Ligand-gated ion channel family ORGANISM: Gloeobacter violaceus (strain ATCC 29082 / PCC 7421) SIMILARITY: Belongs to the ligand-gated ion channel (TC 1.A.9) family. FUNCTION: Cationic channel with similar permeabilities for Na(+) and K(+), that is activated by an increase of the proton concentration on the extracellular side. Displays no permeability for chloride ions. Shows slow kinetics of activation, no desensitization and a single channel conductance of 8 pS. Might contribute to adaptation to external pH change. KEYWORDS: 3D-structure;Cell inner membrane;Cell membrane;Ion channel;Ion transport;Ligand-gated ion channel;Membrane;Potassium;Potassium channel;Potassium transport;Receptor;Reference proteome;Signal;Sodium;Sodium channel;Sodium transport;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell inner membrane ; Multi-pass membrane protein MISCELLANEOUS: The homologous nature of GLIC to eukaryotic nicotinic acetylcholine receptors and other eukaryotic pentameric ligand-gated ion channels, and its sensitivity to general anesthetics, define GLIC as a structural and functional model of signal transduction in the nervous system, also relevant for exploring the molecular basis of anesthetic action." Q7Q2B7,"PROTEIN NAMES: Histone-arginine methyltransferase CARMER (Coactivator-associated arginine methyltransferase 1) (AgCARM1) PROTEIN FAMILY: Class I-like SAM-binding methyltransferase superfamily, Protein arginine N-methyltransferase family ORGANISM: Anopheles gambiae (African malaria mosquito) SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N-methyltransferase family. FUNCTION: Methylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in proteins. May methylate histone H3 at 'Arg-17' and activate transcription via chromatin remodeling. KEYWORDS: Chromatin regulator;Cytoplasm;Methylation;Methyltransferase;Nucleus;Reference proteome;S-adenosyl-L-methionine;Transcription;Transcription regulation;Transferase SUBCELLULAR LOCATION: Cytoplasm Nucleus " Q7RAH3,"PROTEIN NAMES: Calcium-dependent protein kinase 1 PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family, CDPK subfamily ORGANISM: Plasmodium yoelii yoelii SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily. FUNCTION: Calcium-dependent protein kinase which acts as a sensor and effector of intracellular Ca(2+) levels probably in part downstream of cGMP-activated PKG kinase (By similarity). During the liver stage, involved in sporozoite motility and thus in sporozoite invasion of host hepatocytes, probably together with CDPK4 and CDPK5. In the mosquito midgut and during the last stage of male gamete exflagellation, may play a role in the rupture of the host erythrocyte membrane. In the mosquito midgut, required for the differentiation of the zygote into the ookinete by promoting the translational activation of a subset of repressed mRNAs; these mRNAs are kept repressed in the zygote by the DOZI- or CITH-containing mRNP complexes. Dispensable during the asexual blood stage (By similarity). KEYWORDS: ATP-binding;Calcium;Cell membrane;Cell projection;Cilium;Cytoplasm;Flagellum;Host cell membrane;Host membrane;Kinase;Lipoprotein;Membrane;Metal-binding;Myristate;Nucleotide-binding;Palmitate;Phosphoprotein;Reference proteome;Repeat;Serine/threonine-protein kinase;Transferase SUBCELLULAR LOCATION: Membrane ; Lipid-anchor Cell membrane ; Lipid-anchor ; Cytoplasmic side Parasitophorous vacuole membrane ; Lipid-anchor Cytoplasm Cell projection, cilium, flagellum Host cell membrane ; Lipid-anchor Note=Calcium and/or autophosphorylation does not affect membrane localization. " Q7RTM1,"PROTEIN NAMES: Proton channel OTOP1 (Otopetrin-1) (hOtop1) PROTEIN FAMILY: Otopetrin family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the otopetrin family. FUNCTION: Proton-selective ion channel. Biphasically modulated by acid and alkali, mediating proton influx and efflux in response to extracellular acid and base stimulation, respectively. Sour taste receptor, which carries inward currents in response to extracellular acidification (By similarity). Sensor for ammonium chloride (NH(4)Cl) in taste receptor cells. NH(4)Cl acts by increasing the intracellular pH, thereby generating a driving force for proton entry through OTOP1 channel. Might also participate in alkaline sensation. Plays a role in the regulation of Ca(2+) flux in response to purigenic (ATP, ADP and UDP) stimuli, leading to increase in cytosolic Ca(2+) due to influx of extracellular calcium. May play this role by inhibiting P2Y purinoceptor-mediated Ca(2+) release in a Ca(2+)-dependent manner and promote an influx of Ca(2+) in response to ATP. Through this mechanism and possibly others, plays a role in the formation and function of calcium carbonate-based structures in the vestibular system of the inner ear, called otoconia, that sense gravity and linear acceleration. In obesity, may attenuate adipose tissue inflammation, through the negative regulation of IFNG signaling, hence may play an adaptive role in the maintainance of metabolic homeostasis. Following alkali activation, may also be permeable Na(+), K(+), Cs(+) and Li(+) (By similarity). KEYWORDS: Biomineralization;Cell membrane;Cell projection;Hydrogen ion transport;Ion channel;Ion transport;Membrane;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Cell projection, microvillus Note=Found in the gelatinous membrane overlying the inner ear macular epithelium. Also detected in the apical microvilli in inner ear supporting cells. " Q7RTX7,"PROTEIN NAMES: Cation channel sperm-associated protein 4 (CatSper4) PROTEIN FAMILY: Cation channel sperm-associated family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the cation channel sperm-associated (TC 1.A.1.19) family. FUNCTION: Pore-forming subunit of the CatSper complex, a sperm-specific voltage-gated calcium channel that plays a central role in calcium-dependent physiological responses essential for successful fertilization, such as sperm hyperactivation, acrosome reaction and chemotaxis towards the oocyte. KEYWORDS: Alternative splicing;Calcium;Calcium channel;Calcium transport;Cell membrane;Cell projection;Cilium;Developmental protein;Differentiation;Flagellum;Ion channel;Ion transport;Membrane;Reference proteome;Spermatogenesis;Transmembrane;Transmembrane helix;Transport;Voltage-gated channel SUBCELLULAR LOCATION: Cell projection, cilium, flagellum membrane ; Multi-pass membrane protein Note=Specifically located in the principal piece of the sperm tail. " Q7SIG4,"PROTEIN NAMES: Diisopropyl-fluorophosphatase (DFPase) ORGANISM: Loligo vulgaris (Common European squid) FUNCTION: Biological function and substrate unknown. However, it is capable of acting on phosphorus anhydride bonds (such as phosphorus-halide and phosphorus-cyanide) in organophosphorus compounds (including nerve gases). KEYWORDS: 3D-structure;Calcium;Hydrolase;Metal-binding " Q7T1H5,"PROTEIN NAMES: Dead end protein 1 ORGANISM: Danio rerio (Zebrafish) (Brachydanio rerio) FUNCTION: RNA-binding factor that positively regulates gene expression by prohibiting miRNA-mediated gene suppression. Relieves miRNA repression in germline cells. Prohibits the function of several miRNAs by blocking the accessibility of target mRNAs (By similarity). Sequence-specific RNA-binding factor that binds to U-rich regions (URRs) in the 3'untranslated region (3'-UTR) of several mRNAs (By similarity). Does not bind to miRNAs (By similarity). Germline-specific protein required for the primordial germ cell (PGC) survival and migration during early embryonic development. KEYWORDS: Cytoplasm;Developmental protein;Nucleus;Reference proteome;RNA-binding SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=Perinuclear germ granules, also called germ plasm or chromatoid body. " Q7T2B0,PROTEIN NAMES: Choline transporter-like protein 4 (Solute carrier family 44 member 4) PROTEIN FAMILY: CTL (choline transporter-like) family ORGANISM: Danio rerio (Zebrafish) (Brachydanio rerio) SIMILARITY: Belongs to the CTL (choline transporter-like) family. FUNCTION: Choline transporter that seems to play a role in the choline-acetylcholine system and is required to the efferent innervation of hair cells in the olivocochlear bundle for the maintenance of physiological function of outer hair cells and the protection of hair cells from acoustic injury. Also described as a thiamine pyrophosphate transporter (By similarity). KEYWORDS: Antiport;Cell membrane;Glycoprotein;Membrane;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Membrane ; Multi-pass membrane protein Apical cell membrane Q7T384,"PROTEIN NAMES: Sodium-coupled monocarboxylate transporter 2 (Electroneutral sodium monocarboxylate cotransporter) (zSMCTn) (Low-affinity sodium-lactate cotransporter) (Solute carrier family 5 member 12) (zSLC5A12) PROTEIN FAMILY: Sodium:solute symporter (SSF) family ORGANISM: Danio rerio (Zebrafish) (Brachydanio rerio) SIMILARITY: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family. FUNCTION: Acts as an electroneutral and low-affinity sodium (Na(+))-dependent sodium-coupled solute transporter. Catalyzes the transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, nicotinate, propionate, butyrate and beta-D-hydroxybutyrate. KEYWORDS: Cell membrane;Glycoprotein;Ion transport;Membrane;Reference proteome;Sodium;Sodium transport;Symport;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Apical cell membrane ; Multi-pass membrane protein " Q7T3T8,"PROTEIN NAMES: Zygote arrest protein 1 PROTEIN FAMILY: ZAR1 family ORGANISM: Danio rerio (Zebrafish) (Brachydanio rerio) SIMILARITY: Belongs to the ZAR1 family. FUNCTION: mRNA-binding protein required for maternal mRNA storage, translation and degradation during oocyte maturation. Probably promotes formation of some phase-separated membraneless compartment that stores maternal mRNAs in oocytes: acts by undergoing liquid-liquid phase separation upon binding to maternal mRNAs (By similarity). Binds to the 3'-UTR of zona pellucida mRNAs, inhibiting their translation. KEYWORDS: Cytoplasm;Developmental protein;Differentiation;Metal-binding;Oogenesis;Reference proteome;RNA-binding;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cytoplasm, Cytoplasmic ribonucleoprotein granule " Q7TNC4,"PROTEIN NAMES: Putative RNA-binding protein Luc7-like 2 (CGI-74 homolog) PROTEIN FAMILY: Luc7 family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the Luc7 family. FUNCTION: May bind to RNA via its Arg/Ser-rich domain. KEYWORDS: Alternative splicing;Coiled coil;Hydroxylation;Nucleus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Nucleus speckle Nucleus, nucleoplasm Note=Colocalizes with SCNM1 and SNRNP70 in nuclear speckles. " Q7TNJ0,"PROTEIN NAMES: Dendritic cell-specific transmembrane protein (DC-STAMP) (mDC-STAMP) (Dendrocyte-expressed seven transmembrane protein) (Transmembrane 7 superfamily member 4) ORGANISM: Mus musculus (Mouse) FUNCTION: Probable cell surface receptor that plays several roles in cellular fusion, cell differentiation, bone and immune homeostasis. Plays a role in TNFSF11-mediated osteoclastogenesis. Cooperates with OCSTAMP in modulating cell-cell fusion in both osteoclasts and foreign body giant cells (FBGCs). Participates in osteoclast bone resorption. Involved in inducing the expression of tartrate-resistant acid phosphatase in osteoclast precursors. Plays a role in haematopoietic stem cell differentiation of bone marrow cells toward the myeloid lineage. Inhibits the development of neutrophilic granulocytes. Plays also a role in the regulation of dendritic cell (DC) antigen presentation activity by controlling phagocytic activity. Involved in the maintenance of immune self-tolerance and avoidance of autoimmune reactions. KEYWORDS: Alternative splicing;Cell membrane;Differentiation;Endoplasmic reticulum;Endosome;Immunity;Membrane;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Endoplasmic reticulum membrane; Multi-pass membrane protein. Endoplasmic reticulum-Golgi intermediate compartment membrane; Multi-pass membrane protein. Endosome. Note=Localized to the cell surface in osteoclasts and undifferentiated monocytes. Intracellular internalized DCSTAMP is detected in a fraction of RANKL-induced osteoclast precursor. Colocalizes with OS9 in the endoplasmic reticulum (ER) of immature dendritic cell (DC). Translocates from the endoplasmic reticulum to the intermediate/Golgi compartment upon maturation of DC in a OS9-dependent manner. Colocalizes with LAMP1 in endosomes. " Q7TNK7,"PROTEIN NAMES: Ammonium transporter Rh type A (Erythrocyte membrane glycoprotein Rh50) (Rhesus blood group family type A glycoprotein) (Rh family type A glycoprotein) (Rh type A glycoprotein) (CD antigen CD241) PROTEIN FAMILY: Ammonium transporter family, Rh subfamily ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the ammonium transporter (TC 2.A.49) family. Rh subfamily. FUNCTION: Component of the ankyrin-1 complex, a multiprotein complex involved in the stability and shape of the erythrocyte membrane. Heterotrimer with RHCE (RHAG)2(RHCE), that transports ammonium and its related derivative methylammonium, in both neutral and ionic forms, across the erythrocyte membrane. The transport of NH4(+) is electrogenic and masks the NH3 transport. Also, may act as a CO2 channel. Moreover in erythrocyte, regulates RHD membrane expression and is associated with rhesus blood group antigen expression. KEYWORDS: Ammonia transport;Glycoprotein;Membrane;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Membrane ; Multi-pass membrane protein Note=Localization at the plasma membrane is regulated by ANK1. " Q7TNV1,"PROTEIN NAMES: Ceramide synthase (Protein FAM57B) (TLC domain-containing protein 3B) ORGANISM: Mus musculus (Mouse) FUNCTION: Involved in ceramide synthesis. In vitro, isoform 3 stimulates the production of C16-, C18- and C20-ceramides, isoform 1 slightly increases the levels of C18- and C20-ceramides, while isoform 2 exhibits only minimal activity. May interfere with adipogenesis by stimulating ceramide synthesis. KEYWORDS: Alternative promoter usage;Endoplasmic reticulum;Golgi apparatus;Lipid biosynthesis;Lipid metabolism;Membrane;Reference proteome;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: [Isoform 1]: Golgi apparatus membrane ; Multi-pass membrane protein.; SUBCELLULAR LOCATION: [Isoform 2]: Endoplasmic reticulum membrane ; Multi-pass membrane protein.; SUBCELLULAR LOCATION: [Isoform 3]: Endoplasmic reticulum membrane ; Multi-pass membrane protein " Q7UMW2,"PROTEIN NAMES: Bifunctional enzyme CysN/CysC [Includes: Sulfate adenylyltransferase subunit 1 (ATP-sulfurylase large subunit) (Sulfate adenylate transferase) (SAT); Adenylyl-sulfate kinase (APS kinase) (ATP adenosine-5'-phosphosulfate 3'-phosphotransferase)] PROTEIN FAMILY: APS kinase family; TRAFAC class translation factor GTPase superfamily, Classic translation factor GTPase family, CysN/NodQ subfamily ORGANISM: Rhodopirellula baltica (strain DSM 10527 / NCIMB 13988 / SH1) SIMILARITY: In the C-terminal section; belongs to the APS kinase family.; SIMILARITY: In the N-terminal section; belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. CysN/NodQ subfamily. FUNCTION: With CysD forms the ATP sulfurylase (ATPS) that catalyzes the adenylation of sulfate producing adenosine 5'-phosphosulfate (APS) and diphosphate, the first enzymatic step in sulfur assimilation pathway. APS synthesis involves the formation of a high-energy phosphoric-sulfuric acid anhydride bond driven by GTP hydrolysis by CysN coupled to ATP hydrolysis by CysD.; FUNCTION: APS kinase catalyzes the synthesis of activated sulfate. PATHWAY: Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 1/3.; PATHWAY: Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 2/3. KEYWORDS: ATP-binding;GTP-binding;Kinase;Multifunctional enzyme;Nucleotide-binding;Nucleotidyltransferase;Reference proteome;Transferase " Q7X9I0,PROTEIN NAMES: Agamous-like MADS-box protein AGL65 ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Probable transcription factor that forms a heterodimer with the MADS-box protein AGL104 and is involved in the regulation of pollen maturation at the late stages of pollen development and pollen tube growth. KEYWORDS: Alternative splicing;Coiled coil;DNA-binding;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus Q7XA07,"PROTEIN NAMES: Cytoplasmic dynein 2 light intermediate chain 1 (Dynein 1b light intermediate chain) PROTEIN FAMILY: Dynein light intermediate chain family ORGANISM: Chlamydomonas reinhardtii (Chlamydomonas smithii) SIMILARITY: Belongs to the dynein light intermediate chain family. FUNCTION: Functions as a motor for intraflagellar retrograde transport. Functions in flagellar biogenesis. KEYWORDS: Cell membrane;Cell projection;Cilium;Cilium biogenesis/degradation;Cytoplasm;Cytoskeleton;Developmental protein;Dynein;Flagellum;Membrane;Microtubule;Motor protein SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, flagellum basal body. Cell projection, cilium, flagellum membrane; Peripheral membrane protein. Cytoplasm. " Q7XA66,"PROTEIN NAMES: Zinc finger protein ZOP1 (Zinc-finger and OCRE domain-containing protein 1) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Nucleic acid-binding protein that promotes Pol IV-dependent small interfering RNA (siRNA) accumulation, DNA methylation and transcriptional silencing. May possess both RNA-directed DNA methylation (RdDM)-dependent and -independent roles in transcriptional silencing. Acts as a pre-mRNA splicing factor that associates with several typical components of the splicing machinery as well as with Pol II. KEYWORDS: Alternative splicing;Coiled coil;DNA-binding;Metal-binding;mRNA processing;mRNA splicing;Nucleus;Reference proteome;RNA-binding;RNA-mediated gene silencing;Spliceosome;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Nucleus, Cajal body " Q7XJ98,"PROTEIN NAMES: Xyloglucan galactosyltransferase MUR3 (Protein KATAMARI) (Protein MURUS 3) (AtMUR3) (Protein SHORT ROOT IN SALT MEDIUM 3) PROTEIN FAMILY: Glycosyltransferase 47 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the glycosyltransferase 47 family. FUNCTION: Involved in the attachment of the Gal residue on the third xylosyl unit within the XXXG core structure of xyloglucan, the principal glycan that interlaces the cellulose microfibrils in plant cell wall. Associates with other xyloglucan-synthesizing enzymes to form multiprotein complexes for xyloglucan synthesis in the Golgi. Interacts with actin and is required for the proper endomembrane organization and for the cell elongation. Not involved in the trafficking from the endoplasmic reticulum to the vacuoles. Involved in salt stress tolerance. Participates in the control of the expression of genes encoding for proteins involved in reactive oxygen species (ROS) detoxification under salt stress. May contribute to the maintenance of the proper organization of actin microfilaments during salt stress-induced ROS production. KEYWORDS: Glycoprotein;Glycosyltransferase;Golgi apparatus;Membrane;Reference proteome;Signal-anchor;Stress response;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus, Golgi stack membrane ; Single-pass type II membrane protein Golgi apparatus membrane ; Single-pass type II membrane protein " Q7XJM2,"PROTEIN NAMES: Carotenoid cleavage dioxygenase 7, chloroplastic (AtCCD7) (AtNCED7) (Beta,beta-carotene 9',10'-oxygenase) (Protein MORE AXILLARY BRANCHING 3) (Protein MORE AXILLARY GROWTH 3) PROTEIN FAMILY: Carotenoid oxygenase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the carotenoid oxygenase family. FUNCTION: Involved in strigolactones biosynthesis by cleaving asymmetrically a variety of linear and cyclic carotenoids at the 9-10 double bond. Produces one C(13) beta-ionone and the C(27) 10'-apo-beta-carotenal. Strigolactones are hormones that inhibit tillering and shoot branching through the MAX-dependent pathway, contribute to the regulation of shoot architectural response to phosphate-limiting conditions and function as rhizosphere signal that stimulates hyphal branching of arbuscular mycorrhizal fungi and trigger seed germination of root parasitic weeds. No activity on lycopene, lutein, zeaxanthin, violaxanthin or neoxanthin. Probably not involved in abscisic acid biosynthesis. KEYWORDS: Chloroplast;Dioxygenase;Iron;Metal-binding;Oxidoreductase;Plastid;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Plastid, chloroplast MISCELLANEOUS: The branching phenotypes of the max1, ccd7/max3 and ccd8/max4 mutants can be rescued by exogenous treatment with the synthetic strigolactone analogs GR24 and 4BD." Q7XJT7,"PROTEIN NAMES: Probable inactive receptor-like kinase BSK12 (Brassinosteroid-signaling kinase 12) (Protein SHORT SUSPENSOR) PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. FUNCTION: Probable inactive protein kinase that activates the YODA MAP kinase cascade, which regulates the asymmetric first division and embryo polarity, by promoting the elongation of the zygote and the development of its basal daughter cell into the extra-embryonic suspensor. Acts as an adapter at the plasma membrane, possibly by recruiting and binding an activator. KEYWORDS: ATP-binding;Brassinosteroid signaling pathway;Cell membrane;Developmental protein;Kinase;Lipoprotein;Membrane;Myristate;Nucleotide-binding;Palmitate;Reference proteome;Serine/threonine-protein kinase;Transferase SUBCELLULAR LOCATION: Cell membrane ; Lipid-anchor MISCELLANEOUS: BSK12 exerts a paternal effect on embryonic patterning. Transcripts produced but not translated in the sperm cells are delivered to the seed where they become translated, resulting in a transient accumulation of the protein in both products of the double fertilization, the zygote and the central cell." Q7XYY2,"PROTEIN NAMES: Mediator of RNA polymerase II transcription subunit 25 (AtMED25) (Phytochrome and flowering time 1 protein) (Phytochrome and flowering time regulatory protein 1) PROTEIN FAMILY: Mediator complex subunit 25 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the Mediator complex subunit 25 family. FUNCTION: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Positive regulator of shade avoidance and of jasmonate signaling. Acts in repression of PhyB-mediated light signaling and regulates the expression of FLOWERING LOCUS T (FT) and of CONSTANS (CO). KEYWORDS: Alternative splicing;Coiled coil;Flowering;Jasmonic acid signaling pathway;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus MISCELLANEOUS: The affinity between DREB2A and MED25 is reduced when DREB2A is pre-bound to DNA." Q7Y0B9,"PROTEIN NAMES: Serine/threonine-protein kinase SAPK8 (Osmotic stress/abscisic acid-activated protein kinase 8) (stress-activated protein kinase 8) (OsSAPK8) PROTEIN FAMILY: Protein kinase superfamily, Ser/Thr protein kinase family ORGANISM: Oryza sativa subsp. japonica (Rice) SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. FUNCTION: May play a role in signal transduction of hyperosmotic response (Probable). Together with PYL10, PP2C53 and SAPK10, may form an abscisic acid (ABA) signaling module involved in stress response. KEYWORDS: Abscisic acid signaling pathway;ATP-binding;Cytoplasm;Kinase;Nucleotide-binding;Nucleus;Phosphoprotein;Reference proteome;Serine/threonine-protein kinase;Transferase SUBCELLULAR LOCATION: Cytoplasm, cytosol Nucleus " Q7Y1W1,"PROTEIN NAMES: Protein TIC 56, chloroplastic (Translocon at the inner envelope membrane of chloroplasts 56) (AtTIC56) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Involved in protein precursor import into chloroplasts. May be part of an intermediate translocation complex acting as a protein-conducting channel at the inner envelope. KEYWORDS: Chloroplast;Membrane;Plastid;Plastid inner membrane;Protein transport;Reference proteome;Transit peptide;Transport SUBCELLULAR LOCATION: Plastid, chloroplast inner membrane " Q7YRJ7,"PROTEIN NAMES: Transcription factor SOX-9 ORGANISM: Canis lupus familiaris (Dog) (Canis familiaris) FUNCTION: Transcription factor that plays a key role in chondrocytes differentiation and skeletal development. Specifically binds the 5'-ACAAAG-3' DNA motif present in enhancers and super-enhancers and promotes expression of genes important for chondrogenesis, including cartilage matrix protein-coding genes COL2A1, COL4A2, COL9A1, COL11A2 and ACAN, SOX5 and SOX6. Also binds to some promoter regions. Plays a central role in successive steps of chondrocyte differentiation. Absolutely required for precartilaginous condensation, the first step in chondrogenesis during which skeletal progenitors differentiate into prechondrocytes. Together with SOX5 and SOX6, required for overt chondrogenesis when condensed prechondrocytes differentiate into early stage chondrocytes, the second step in chondrogenesis. Later, required to direct hypertrophic maturation and block osteoblast differentiation of growth plate chondrocytes: maintains chondrocyte columnar proliferation, delays prehypertrophy and then prevents osteoblastic differentiation of chondrocytes by lowering beta-catenin (CTNNB1) signaling and RUNX2 expression. Also required for chondrocyte hypertrophy, both indirectly, by keeping the lineage fate of chondrocytes, and directly, by remaining present in upper hypertrophic cells and transactivating COL10A1 along with MEF2C. Low lipid levels are the main nutritional determinant for chondrogenic commitment of skeletal progenitor cells: when lipids levels are low, FOXO (FOXO1 and FOXO3) transcription factors promote expression of SOX9, which induces chondrogenic commitment and suppresses fatty acid oxidation. Mechanistically, helps, but is not required, to remove epigenetic signatures of transcriptional repression and deposit active promoter and enhancer marks at chondrocyte-specific genes. Acts in cooperation with the Hedgehog pathway-dependent GLI (GLI1 and GLI3) transcription factors. In addition to cartilage development, also acts as a regulator of proliferation and differentiation in epithelial stem/progenitor cells: involved in the lung epithelium during branching morphogenesis, by balancing proliferation and differentiation and regulating the extracellular matrix. Controls epithelial branching during kidney development. KEYWORDS: Acetylation;Activator;Differentiation;DNA-binding;Isopeptide bond;Nucleus;Phosphoprotein;Reference proteome;Transcription;Transcription regulation;Ubl conjugation SUBCELLULAR LOCATION: Nucleus " Q7YSW8,"PROTEIN NAMES: Serine/threonine-protein phosphatase 2B catalytic subunit (Calcineurin subunit A) PROTEIN FAMILY: PPP phosphatase family, PP-2B subfamily ORGANISM: Dictyostelium discoideum (Social amoeba) SIMILARITY: Belongs to the PPP phosphatase family. PP-2B subfamily. FUNCTION: Catalytic subunit of calcineurin, a calcium-dependent, calmodulin stimulated protein phosphatase which plays an essential role in the transduction of intracellular Ca(2+)-mediated signals. KEYWORDS: Calmodulin-binding;Coiled coil;Hydrolase;Iron;Metal-binding;Protein phosphatase;Reference proteome;Zinc " Q7YTB0,"PROTEIN NAMES: Ecdysteroid-phosphate phosphatase (EPPase) ORGANISM: Bombyx mori (Silk moth) FUNCTION: Steroid phosphatase which catalyzes the conversion of inactive phosphorylated ecdysteroids into their active forms. Shows high activity towards ecdysone 22-phosphate (E22P). Has lower activity towards other ecdysteriod phosphates including 20-hydroxyecdysone 22-phosphate (20E22P) and 2-deoxyecdysone 22-phosphate (2dE22P). Also has protein tyrosine phosphatase activity. KEYWORDS: 3D-structure;Alternative splicing;Cytoplasm;Direct protein sequencing;Hydrolase;Protein phosphatase;Reference proteome;SH3 domain SUBCELLULAR LOCATION: Cytoplasm, cytosol Note=In 72 hr non-diapause eggs, detected mainly in the cystol surrounding the nucleus of yolk cells. " Q7YXU4,"PROTEIN NAMES: Copine-A PROTEIN FAMILY: Copine family ORGANISM: Dictyostelium discoideum (Social amoeba) SIMILARITY: Belongs to the copine family. FUNCTION: Required for cytokinesis, contractile vacuole function and development. KEYWORDS: Calcium;Cytoplasm;Membrane;Metal-binding;Reference proteome;Repeat SUBCELLULAR LOCATION: Cytoplasm Membrane ; Peripheral membrane protein Note=In starved cells it binds transiently and in a calcium-dependent manner to the plasma membrane and intracellular vacuoles. In some cells, the transient membrane localization is observed to occur multiple times in an oscillatory manner over several minutes. Also found to be associated to the plasma membrane, contractile vacuoles, organelles of the endolysosomal pathway and phagosomes. " Q7Z139,"PROTEIN NAMES: Ceramide synthase hyl-2 (HYL-2) PROTEIN FAMILY: Sphingosine N-acyltransferase family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the sphingosine N-acyltransferase family. FUNCTION: Catalyzes the acylation of sphingoid bases to form ceramides. Sphingolipids from Caenorhabditis elegans contain exclusively isosphingoid bases. Exhibits substrate preference for fatty acyl-coA chains containing 20 to 22 carbons. Required for adaptation of the nematode to anoxia. Anoxia tolerance may require one or more of the ceramide species that are either specifically or preferentially synthesized by HYL-2, and seems to be affected by a pathway that is parallel to that involving daf-2. PATHWAY: Lipid metabolism; sphingolipid metabolism. KEYWORDS: Glycoprotein;Lipid biosynthesis;Lipid metabolism;Membrane;Reference proteome;Sphingolipid metabolism;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Multi-pass membrane protein " Q7Z2K6,"PROTEIN NAMES: Endoplasmic reticulum metallopeptidase 1 (Felix-ina) PROTEIN FAMILY: Peptidase M28 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the peptidase M28 family. FUNCTION: Within the ovary, required for the organization of somatic cells and oocytes into discrete follicular structures. KEYWORDS: Acetylation;Alternative splicing;Disulfide bond;Endoplasmic reticulum;Glycoprotein;Hydrolase;Membrane;Metal-binding;Metalloprotease;Protease;Reference proteome;Transmembrane;Transmembrane helix;Zinc SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein " Q7Z3E5,"PROTEIN NAMES: LisH domain-containing protein ARMC9 (Armadillo repeat-containing protein 9) (Melanoma/melanocyte-specific tumor antigen KU-MEL-1) (NS21) ORGANISM: Homo sapiens (Human) FUNCTION: Involved in ciliogenesis. It is required for appropriate acetylation and polyglutamylation of ciliary microtubules, and regulation of cilium length. Acts as a positive regulator of hedgehog (Hh)signaling (By similarity). May participate in the trafficking and/or retention of GLI2 and GLI3 proteins at the ciliary tip (By similarity). KEYWORDS: Alternative splicing;Cell projection;Ciliopathy;Cilium biogenesis/degradation;Coiled coil;Cytoplasm;Cytoskeleton;Joubert syndrome;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, cilium basal body Cell projection, cilium Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole Note=Localized to the proximal region in cilia. Stimulation of Hh signaling leads to redistribution of ARMC9 toward the ciliary tip within 6 hours, follow by a gradual return to its original proximal location (By similarity). Localizes to the daughter centriole of the primary cilium in RPE1 cells. " Q7Z3H0,"PROTEIN NAMES: Photoreceptor ankyrin repeat protein (Ankyrin repeat domain-containing protein 33) ORGANISM: Homo sapiens (Human) FUNCTION: Acts as a transcriptional repressor for CRX-activated photoreceptor gene regulation. KEYWORDS: Alternative splicing;ANK repeat;Cytoplasm;Nucleus;Reference proteome;Repeat SUBCELLULAR LOCATION: Cytoplasm, cytosol Nucleus " Q7Z429,"PROTEIN NAMES: Protein lifeguard 1 (Glutamate [NMDA] receptor-associated protein 1) (NMDA receptor glutamate-binding subunit) (Putative MAPK-activating protein PM02) (Transmembrane BAX inhibitor motif-containing protein 3) PROTEIN FAMILY: BI1 family, LFG subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the BI1 family. LFG subfamily. FUNCTION: Potential apoptotic regulator. KEYWORDS: Membrane;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Multi-pass membrane protein " Q7Z465,PROTEIN NAMES: Bcl-2/adenovirus E1B 19 kDa-interacting protein 2-like protein ORGANISM: Homo sapiens (Human) FUNCTION: May be a bridge molecule between BCL2 and ARHGAP1/CDC42 in promoting cell death. KEYWORDS: Alternative splicing;Apoptosis;Reference proteome Q7Z4H7,"PROTEIN NAMES: HAUS augmin-like complex subunit 6 PROTEIN FAMILY: HAUS6 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the HAUS6 family. FUNCTION: Contributes to mitotic spindle assembly, maintenance of centrosome integrity and completion of cytokinesis as part of the HAUS augmin-like complex. Promotes the nucleation of microtubules from the spindle through recruitment of NEDD1 and gamma-tubulin. KEYWORDS: 3D-structure;Alternative splicing;Cell cycle;Cell division;Coiled coil;Cytoplasm;Cytoskeleton;Microtubule;Mitosis;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton Cytoplasm, cytoskeleton, spindle Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Note=Localizes to interphase centrosomes and to mitotic spindle microtubules. " Q7Z5L7,"PROTEIN NAMES: Podocan PROTEIN FAMILY: Small leucine-rich proteoglycan (SLRP) family, SLRP class V subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class V subfamily. FUNCTION: Negatively regulates cell proliferation and cell migration. KEYWORDS: Alternative splicing;Cytoplasm;Extracellular matrix;Glycoprotein;Leucine-rich repeat;Reference proteome;Repeat;Secreted;Signal SUBCELLULAR LOCATION: [Isoform 1]: Secreted, extracellular space, extracellular matrix.; SUBCELLULAR LOCATION: [Isoform 2]: Cytoplasm.; SUBCELLULAR LOCATION: [Isoform 3]: Cytoplasm.; SUBCELLULAR LOCATION: [Isoform 4]: Cytoplasm MISCELLANEOUS: [Isoform 2]: No signal peptide.; MISCELLANEOUS: [Isoform 3]: No signal peptide.; MISCELLANEOUS: [Isoform 4]: No signal peptide." Q7Z628,PROTEIN NAMES: Neuroepithelial cell-transforming gene 1 protein (Proto-oncogene p65 Net1) (Rho guanine nucleotide exchange factor 8) ORGANISM: Homo sapiens (Human) FUNCTION: Acts as a guanine nucleotide exchange factor (GEF) for RhoA GTPase. May be involved in activation of the SAPK/JNK pathway Stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death. KEYWORDS: 3D-structure;Acetylation;Alternative splicing;Cytoplasm;Guanine-nucleotide releasing factor;Nucleus;Phosphoprotein;Proto-oncogene;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Nucleus Q7Z699,"PROTEIN NAMES: Sprouty-related, EVH1 domain-containing protein 1 (Spred-1) (hSpred1) ORGANISM: Homo sapiens (Human) FUNCTION: Tyrosine kinase substrate that inhibits growth-factor-mediated activation of MAP kinase (By similarity). Negatively regulates hematopoiesis of bone marrow (By similarity). Inhibits fibroblast growth factor (FGF)-induced retinal lens fiber differentiation, probably by inhibiting FGF-mediated phosphorylation of ERK1/2 (By similarity). Attenuates actin stress fiber formation via inhibition of TESK1-mediated phosphorylation of cofilin. Inhibits TGFB-induced epithelial-to-mesenchymal transition in lens epithelial cells (By similarity). KEYWORDS: 3D-structure;Acetylation;Cell membrane;Disease variant;Lipoprotein;Membrane;Methylation;Nucleus;Palmitate;Phosphoprotein;Reference proteome;Ubl conjugation SUBCELLULAR LOCATION: Cell membrane ; Peripheral membrane protein Membrane, caveola ; Peripheral membrane protein Nucleus Note=Localized in cholesterol-rich membrane raft/caveola fractions. " Q7Z6M4,"PROTEIN NAMES: Transcription termination factor 4, mitochondrial (Mitochondrial transcription termination factor 4) (mTERF domain-containing protein 2) [Cleaved into: mTERF domain-containing protein 2 processed] PROTEIN FAMILY: MTERF family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the mTERF family. FUNCTION: Regulator of mitochondrial ribosome biogenesis and translation. Binds to mitochondrial ribosomal RNAs 16S, 12S and 7S and targets NSUN4 RNA methyltransferase to the mitochondrial large ribosomal subunit (39S). KEYWORDS: 3D-structure;Direct protein sequencing;Mitochondrion;Reference proteome;Repeat;RNA-binding;rRNA processing;Transit peptide SUBCELLULAR LOCATION: Mitochondrion " Q7Z7M8,"PROTEIN NAMES: UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 8 (BGnT-8) (Beta-1,3-Gn-T8) (Beta-1,3-N-acetylglucosaminyltransferase 8) (Beta3Gn-T8) PROTEIN FAMILY: Glycosyltransferase 31 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the glycosyltransferase 31 family. FUNCTION: Beta-1,3-N-acetylglucosaminyltransferase that plays a role in the elongation of specific branch structures of multiantennary N-glycans. Has strong activity towards tetraantennary N-glycans and 2,6 triantennary glycans. PATHWAY: Protein modification; protein glycosylation. KEYWORDS: Glycoprotein;Glycosyltransferase;Golgi apparatus;Membrane;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus membrane ; Single-pass type II membrane protein " Q7Z7M9,"PROTEIN NAMES: Polypeptide N-acetylgalactosaminyltransferase 5 (Polypeptide GalNAc transferase 5) (GalNAc-T5) (pp-GaNTase 5) (Protein-UDP acetylgalactosaminyltransferase 5) (UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 5) PROTEIN FAMILY: Glycosyltransferase 2 family, GalNAc-T subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily. FUNCTION: Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Has activity toward EA2 peptide substrate, but has a weak activity toward Muc2 or Muc1b substrates (By similarity). PATHWAY: Protein modification; protein glycosylation. KEYWORDS: Disulfide bond;Glycoprotein;Glycosyltransferase;Golgi apparatus;Lectin;Manganese;Membrane;Metal-binding;Phosphoprotein;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus membrane ; Single-pass type II membrane protein " Q7Z7N9,PROTEIN NAMES: Transmembrane protein 179B PROTEIN FAMILY: TMEM179 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the TMEM179 family. KEYWORDS: Membrane;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Multi-pass membrane protein Q7ZWK8,"PROTEIN NAMES: Proton channel OTOP1 (Otopetrin-1) PROTEIN FAMILY: Otopetrin family ORGANISM: Danio rerio (Zebrafish) (Brachydanio rerio) SIMILARITY: Belongs to the otopetrin family. FUNCTION: Proton-selective ion channel. Biphasically modulated by acid and alkali, mediating proton influx and efflux in response to extracellular acid and base stimulation, respectively (By similarity). May be involved in acid and base perception (By similarity). Sensor for ammonium chloride (NH(4)Cl) in taste receptor cells. NH(4)Cl acts by increasing the intracellular pH, thereby generating a driving force for proton entry through OTOP1 channel. Plays a role in the regulation of Ca(2+) flux in response to purigenic (ATP, ADP and UDP) stimuli, leading to increase in cytosolic Ca(2+) due to influx of extracellular calcium. May play this role by inhibiting P2Y purinoceptor-mediated Ca(2+) release in a Ca(2+)-dependent manner and promote an influx of Ca(2+) in response to ATP. Through this mechanism and possibly others, plays a role in the formation and function of calcium carbonate-based structures in the vestibular system of the inner ear, called otoconia, that sense gravity and linear acceleration. KEYWORDS: Biomineralization;Cell membrane;Cell projection;Hydrogen ion transport;Ion channel;Ion transport;Membrane;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Cell projection, microvillus Note=Found in the gelatinous membrane overlying the inner ear macular epithelium. Also detected in the apical microvilli in inner ear supporting cells. " Q7ZY18,"PROTEIN NAMES: Zygotic DNA replication licensing factor mcm6-B (Zygotic minichromosome maintenance protein 6-B) (zMCM6-B) (zMCM6b) PROTEIN FAMILY: MCM family ORGANISM: Xenopus laevis (African clawed frog) SIMILARITY: Belongs to the MCM family. FUNCTION: Acts as a component of the mcm2-7 complex (mcm complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the mcm2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. The existence of maternal and zygotic forms of mcm3 and mcm6 suggests that specific forms of mcm2-7 complexes may be used during different stages of development. May replace mmcm6 in the mcm2-7 complex. KEYWORDS: ATP-binding;Cell cycle;DNA replication;DNA-binding;Helicase;Hydrolase;Metal-binding;Nucleotide-binding;Nucleus;Reference proteome;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Note=Associated with chromatin before the formation of nuclei and detaches from it as DNA replication progresses. " Q801G2,"PROTEIN NAMES: Membrane progestin receptor alpha-B (mPR alpha) (Progestin and adipoQ receptor family member VII, b) PROTEIN FAMILY: ADIPOR family ORGANISM: Danio rerio (Zebrafish) (Brachydanio rerio) SIMILARITY: Belongs to the ADIPOR family. FUNCTION: Steroid membrane receptor. Signals upon progestin binding, resulting in rapid activation of MAPK and down-regulation of adenylyl cyclase activity. Interacts with steroids with varying degrees of affinity, showing specificity for activation by the maturation-inducing steroid (MIS) 4-pregnen-17,20beta-diol-3-one (17,20beta-DHP). Capable of mediating progestin-induced oocyte maturation. KEYWORDS: Cell membrane;Developmental protein;Differentiation;Lipid-binding;Membrane;Oogenesis;Receptor;Reference proteome;Steroid-binding;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein " Q801X7,PROTEIN NAMES: Galactose-binding lectin l-1 (Galectin) (Ajl-1) ORGANISM: Anguilla japonica (Japanese eel) FUNCTION: Involved in host defense at the body surface. Causes agglutination of the Gram-positive bacterium S.difficile. Possesses calcium-independent hemagglutinating activity. KEYWORDS: Direct protein sequencing;Glycoprotein;Hemagglutinin;Lectin;Secreted SUBCELLULAR LOCATION: Secreted MISCELLANEOUS: Binds beta-galactoside inhibiting its haemagglutinating activity. Q802V6,"PROTEIN NAMES: Monoacylglycerol lipase ABHD2 (2-arachidonoylglycerol hydrolase) (Abhydrolase domain-containing protein 2-A) (Acetylesterase) (Triacylglycerol lipase) PROTEIN FAMILY: AB hydrolase superfamily, AB hydrolase 4 family ORGANISM: Danio rerio (Zebrafish) (Brachydanio rerio) SIMILARITY: Belongs to the AB hydrolase superfamily. AB hydrolase 4 family. FUNCTION: Progesterone-dependent acylglycerol lipase that catalyzes hydrolysis of endocannabinoid arachidonoylglycerol (AG) from cell membrane. Acts as a progesterone receptor: progesterone-binding activates the acylglycerol lipase activity, mediating degradation of 1-arachidonoylglycerol (1AG) and 2-arachidonoylglycerol (2AG) to glycerol and arachidonic acid (AA). Also displays an ester hydrolase activity against acetyl ester, butanoate ester and hexadecanoate ester. Plays a key role in sperm capacitation in response to progesterone by mediating degradation of 2AG, an inhibitor of the sperm calcium channel CatSper, leading to calcium influx via CatSper and sperm activation (By similarity). May also play a role in smooth muscle cells migration (By similarity). KEYWORDS: Cell membrane;Glycoprotein;Hydrolase;Lipid degradation;Lipid metabolism;Membrane;Reference proteome;Serine esterase;Signal-anchor;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type II membrane protein " Q80TN5,"PROTEIN NAMES: Palmitoyltransferase ZDHHC17 (Acyltransferase ZDHHC17) (DHHC domain-containing cysteine-rich protein 17) (DHHC-17) (Huntingtin-interacting protein 14) (Zinc finger DHHC domain-containing protein 17) PROTEIN FAMILY: DHHC palmitoyltransferase family, AKR/ZDHHC17 subfamily ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the DHHC palmitoyltransferase family. AKR/ZDHHC17 subfamily. FUNCTION: Palmitoyltransferase that catalyzes the addition of palmitate onto various protein substrates and is involved in a variety of cellular processes. Has no stringent fatty acid selectivity and in addition to palmitate can also transfer onto target proteins myristate from tetradecanoyl-CoA and stearate from octadecanoyl-CoA. Palmitoyltransferase specific for a subset of neuronal proteins, including SNAP25, DLG4/PSD95, GAD2, SYT1 and HTT. Also palmitoylates neuronal protein GPM6A as well as SPRED1 and SPRED3 (By similarity). Could also play a role in axonogenesis through the regulation of NTRK1 and the downstream ERK1/ERK2 signaling cascade. May be involved in the sorting or targeting of critical proteins involved in the initiating events of endocytosis at the plasma membrane (By similarity). May play a role in Mg(2+) transport (By similarity). Could also palmitoylate DNAJC5 and regulate its localization to the Golgi membrane. Palmitoylates CASP6, thereby preventing its dimerization and subsequent activation. KEYWORDS: Acyltransferase;Alternative splicing;ANK repeat;Cell membrane;Cell projection;Cytoplasmic vesicle;Golgi apparatus;Lipoprotein;Membrane;Palmitate;Reference proteome;Repeat;Synapse;Transferase;Transmembrane;Transmembrane helix;Tumor suppressor SUBCELLULAR LOCATION: Golgi apparatus membrane ; Multi-pass membrane protein Cytoplasmic vesicle membrane ; Multi-pass membrane protein Presynaptic cell membrane ; Multi-pass membrane protein Note=Low extracellular Mg(2+) induces increase in Golgi and in post-Golgi membrane vesicles. " Q80UN9,"PROTEIN NAMES: tRNA dimethylallyltransferase (Isopentenyl-diphosphate:tRNA isopentenyltransferase) (IPP transferase) (IPPT) (tRNA isopentenyltransferase) (IPTase) PROTEIN FAMILY: IPP transferase family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the IPP transferase family. FUNCTION: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 of both cytosolic and mitochondrial tRNAs, leading to the formation of N6-(dimethylallyl)adenosine (i6A37). Mediates modification of a limited subset of tRNAs: tRNA(Ser)(AGA), tRNA(Ser)(CGA), tRNA(Ser)(UGA), as well as partial modification of the selenocysteine tRNA(Ser)(UCA) (By similarity). TRIT1 is therefore required for selenoprotein expression. KEYWORDS: Alternative splicing;ATP-binding;Cytoplasm;Metal-binding;Mitochondrion;Nucleotide-binding;Nucleus;Phosphoprotein;Reference proteome;Transferase;Transit peptide;tRNA processing;Zinc;Zinc-finger SUBCELLULAR LOCATION: [Isoform 1]: Mitochondrion.; SUBCELLULAR LOCATION: [Isoform 2]: Cytoplasm Nucleus " Q80UW0,PROTEIN NAMES: Heparan-sulfate 6-O-sulfotransferase 2 (HS6ST-2) (mHS6ST-2) PROTEIN FAMILY: Sulfotransferase 6 family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the sulfotransferase 6 family. FUNCTION: 6-O-sulfation enzyme which catalyzes the transfer of sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to position 6 of the N-sulfoglucosamine residue (GlcNS) of heparan sulfate. KEYWORDS: Alternative splicing;Glycoprotein;Membrane;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Single-pass type II membrane protein Q80V63,PROTEIN NAMES: PR domain zinc finger protein 4 (PR domain-containing protein 4) PROTEIN FAMILY: Class V-like SAM-binding methyltransferase superfamily ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the class V-like SAM-binding methyltransferase superfamily. FUNCTION: May function as a transcription factor involved in cell differentiation. KEYWORDS: DNA-binding;Metal-binding;Methyltransferase;Nucleus;Reference proteome;Repeat;S-adenosyl-L-methionine;Transcription;Transcription regulation;Transferase;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Q80V72,"PROTEIN NAMES: Inositol hexakisphosphate kinase 2 (InsP6 kinase 2) (P(i)-uptake stimulator) (PiUS) PROTEIN FAMILY: Inositol phosphokinase (IPK) family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the inositol phosphokinase (IPK) family. FUNCTION: Converts inositol hexakisphosphate (InsP6) to diphosphoinositol pentakisphosphate (InsP7/PP-InsP5) (By similarity). May play a role in the regulation of Na(+)-dependent phosphate cotransport, possibly via its role in diphosphoinositol pentakisphosphate (InsP7/PP-InsP5) biosynthesis (By similarity). PATHWAY: Phospholipid metabolism; phosphatidylinositol metabolism. KEYWORDS: ATP-binding;Kinase;Lipid metabolism;Nucleotide-binding;Nucleus;Phospholipid metabolism;Reference proteome;Transferase SUBCELLULAR LOCATION: Nucleus " Q80V85,"PROTEIN NAMES: E3 ubiquitin-protein ligase TRIM62 (Tripartite motif-containing protein 62) PROTEIN FAMILY: TRIM/RBCC family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the TRIM/RBCC family. FUNCTION: E3 ubiquitin ligase that plays a role in antifungal immunity by mediating 'Lys-27'-linked ubiquitination of CARD9 downstream of C-type lectin receptors; leading to CARD9 activation, followed by activation of NF-kappa-B and MAP kinase p38 pathways (By similarity). E3 ubiquitin ligase activity is dependent on E2 ubiquitin-conjugating enzyme UBE2D2 (By similarity). PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Coiled coil;Cytoplasm;Immunity;Innate immunity;Metal-binding;Reference proteome;Transferase;Ubl conjugation;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cytoplasm " Q80VJ8,"PROTEIN NAMES: Protein KASH5 (Coiled-coil domain-containing protein 155) (KASH domain-containing protein 5) ORGANISM: Mus musculus (Mouse) FUNCTION: As a component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex, involved in the connection between the nuclear lamina and the cytoskeleton. The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning. Required for telomere attachment to nuclear envelope in the prophase of meiosis and for rapid telomere prophase movements implicating a SUN1/2:KASH5 LINC complex in which SUN1 and SUN2 seem to act at least partial redundantly. Required for homolog pairing during meiotic prophase in spermatocytes and probably oocytes. Essential for male and female gametogenesis. Recruits cytoplasmic dynein to telomere attachment sites at the nuclear envelope in spermatocytes. In oocytes is involved in meiotic resumption and spindle formation. KEYWORDS: Chromosome;Coiled coil;Meiosis;Membrane;Nucleus;Reference proteome;Telomere;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Nucleus outer membrane ; Single-pass type IV membrane protein ; Cytoplasmic side Nucleus Chromosome, telomere Nucleus envelope Note=Localized exclusively at telomeres from the leptotene to diplotene stages. Colocalizes with SUN2 at sites of telomere attachment in meiocytes. At oocyte MI stage localized around the spindle, at MII stage localized to the spindle poles. " Q80WD0,"PROTEIN NAMES: Reticulon-4 receptor-like 1 (Nogo receptor-like 2) (Nogo-66 receptor homolog 2) (Nogo-66 receptor-related protein 3) (NgR3) PROTEIN FAMILY: Nogo receptor family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the Nogo receptor family. FUNCTION: Cell surface receptor. Plays a functionally redundant role in postnatal brain development and in regulating axon regeneration in the adult central nervous system. Contributes to normal axon migration across the brain midline and normal formation of the corpus callosum. Protects motoneurons against apoptosis; protection against apoptosis is probably mediated by MAG. Plays a role in inhibiting neurite outgrowth and axon regeneration via its binding to neuronal chondroitin sulfate proteoglycans. Binds heparin (By similarity). Like other family members, plays a role in restricting the number dendritic spines and the number of synapses that are formed during brain development. Signaling mediates activation of Rho and downstream reorganization of the actin cytoskeleton. KEYWORDS: Alternative splicing;Cell membrane;Cell projection;Glycoprotein;GPI-anchor;Leucine-rich repeat;Lipoprotein;Membrane;Receptor;Reference proteome;Repeat;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Lipid-anchor, GPI-anchor Membrane raft Perikaryon Cell projection Note=Localized to the surface of neurons, including axons. " Q80WM9,"PROTEIN NAMES: Tumor necrosis factor receptor superfamily member 14 (Herpes virus entry mediator A) (Herpesvirus entry mediator A) (HveA) (Tumor necrosis factor receptor-like 2) (TR2) (CD antigen CD270) PROTEIN FAMILY: Tumor necrosis factor receptor superfamily ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the tumor necrosis factor receptor superfamily. FUNCTION: Receptor for four distinct ligands: The TNF superfamily members TNFSF14/LIGHT and homotrimeric LTA/lymphotoxin-alpha and the immunoglobulin superfamily members BTLA and CD160, altogether defining a complex stimulatory and inhibitory signaling network (By similarity). Signals via the TRAF2-TRAF3 E3 ligase pathway to promote immune cell survival and differentiation. Participates in bidirectional cell-cell contact signaling between antigen presenting cells and lymphocytes. In response to ligation of TNFSF14/LIGHT, delivers costimulatory signals to T cells, promoting cell proliferation and effector functions (By similarity). Interacts with CD160 on NK cells, enhancing IFNG production and anti-tumor immune response. In the context of bacterial infection, acts as a signaling receptor on epithelial cells for CD160 from intraepithelial lymphocytes, triggering the production of antimicrobial proteins and pro-inflammatory cytokines. Upon binding to CD160 on activated CD4+ T cells, down-regulates CD28 costimulatory signaling, restricting memory and alloantigen-specific immune response (By similarity). May interact in cis (on the same cell) or in trans (on other cells) with BTLA. In cis interactions, appears to play an immune regulatory role inhibiting in trans interactions in naive T cells to maintain a resting state. In trans interactions, can predominate during adaptive immune response to provide survival signals to effector T cells. KEYWORDS: 3D-structure;Adaptive immunity;Cell membrane;Disulfide bond;Glycoprotein;Host cell receptor for virus entry;Host-virus interaction;Immunity;Innate immunity;Membrane;Phosphoprotein;Receptor;Reference proteome;Repeat;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein " Q80WV3,"PROTEIN NAMES: Carbohydrate sulfotransferase 2 (Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 2) (GST-2) (N-acetylglucosamine 6-O-sulfotransferase 1) (GlcNAc6ST-1) (Gn6st-1) PROTEIN FAMILY: Sulfotransferase 1 family, Gal/GlcNAc/GalNAc subfamily ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the sulfotransferase 1 family. Gal/GlcNAc/GalNAc subfamily. FUNCTION: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues within keratan-like structures on N-linked glycans and within mucin-associated glycans that can ultimately serve as SELL ligands. SELL ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation. Participates in biosynthesis of the SELL ligand sialyl 6-sulfo Lewis X and in lymphocyte homing to Peyer patches. Has no activity toward O-linked sugars. Its substrate specificity may be influenced by its subcellular location. Sulfates GlcNAc residues at terminal, non-reducing ends of oligosaccharide chains. PATHWAY: Protein modification; carbohydrate sulfation. KEYWORDS: Carbohydrate metabolism;Disulfide bond;Glycoprotein;Golgi apparatus;Inflammatory response;Membrane;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus, trans-Golgi network membrane ; Single-pass type II membrane protein " Q80X41,"PROTEIN NAMES: Serine/threonine-protein kinase VRK1 (Serine/threonine-protein kinase 51PK) (Vaccinia-related kinase 1) PROTEIN FAMILY: Protein kinase superfamily, CK1 Ser/Thr protein kinase family, VRK subfamily ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. VRK subfamily. FUNCTION: Serine/threonine kinase involved in the regulation of key cellular processes including the cell cycle, nuclear condensation, transcription regulation, and DNA damage response. Controls chromatin organization and remodeling by mediating phosphorylation of histone H3 on 'Thr-4' and histone H2AX (H2aXT4ph) (By similarity). It also phosphorylates KAT5 in response to DNA damage, promoting KAT5 association with chromatin and histone acetyltransferase activity. Is involved in the regulation of cell cycle progression of neural progenitors, and is required for proper cortical neuronal migration. Is involved in neurite elongation and branching in motor neurons, and has an essential role in Cajal bodies assembly, acting through COIL phosphorylation and the control of coilin degradation (By similarity). Involved in Golgi disassembly during the cell cycle: following phosphorylation by PLK3 during mitosis, required to induce Golgi fragmentation (By similarity). Phosphorylates BANF1: disrupts its ability to bind DNA, reduces its binding to LEM domain-containing proteins and causes its relocalization from the nucleus to the cytoplasm (By similarity). Phosphorylates TP53BP1 and p53/TP53 on 'Thr-18', preventing the interaction between p53/TP53 and MDM2 (By similarity). Phosphorylates ATF2 which activates its transcriptional activity (By similarity). Phosphorylates JUN (By similarity). KEYWORDS: Alternative splicing;ATP-binding;Cell cycle;Cell division;Cytoplasm;Direct protein sequencing;Isopeptide bond;Kinase;Mitosis;Nucleotide-binding;Nucleus;Phosphoprotein;Reference proteome;Serine/threonine-protein kinase;Transferase;Ubl conjugation SUBCELLULAR LOCATION: Nucleus Cytoplasm Nucleus, Cajal body Note=Enriched on chromatin during mitotic chromatin condensation. " Q80X44,PROTEIN NAMES: Zinc finger and BTB domain-containing protein 24 (Bone morphogenetic protein-induced factor 1) (Brain-specific protein 1) (Zinc finger protein 450) PROTEIN FAMILY: Krueppel C2H2-type zinc-finger protein family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein family. FUNCTION: May be involved in BMP2-induced transcription. KEYWORDS: 3D-structure;Alternative splicing;Direct protein sequencing;DNA-binding;Metal-binding;Nucleus;Reference proteome;Repeat;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Q80XF7,"PROTEIN NAMES: Gap junction gamma-2 protein (Connexin-47) (Cx47) (Gap junction alpha-12 protein) PROTEIN FAMILY: Connexin family, Gamma-type subfamily ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the connexin family. Gamma-type subfamily. FUNCTION: One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a role in myelination in central and peripheral nervous systems (By similarity). KEYWORDS: Cell junction;Cell membrane;Gap junction;Membrane;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Cell junction, gap junction. " Q80YW5,PROTEIN NAMES: B box and SPRY domain-containing protein ORGANISM: Mus musculus (Mouse) FUNCTION: May regulate epithelial calcium transport by inhibiting TRPV5 activity. KEYWORDS: Alternative splicing;Calcium;Calcium transport;Cytoplasm;Ion transport;Membrane;Metal-binding;Reference proteome;Transport;Vitamin D;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cytoplasm Membrane ; Peripheral membrane protein Note=Apical domain of kidney distal tubular cells. Q80ZC8,"PROTEIN NAMES: Killer cell lectin-like receptor subfamily E member 1 ORGANISM: Rattus norvegicus (Rat) FUNCTION: Lectin-like receptor for natural killer (NK) cells. Can either inhibit or activate NK cell cytotoxic activity, depending on its binding partner. Heterodimer formation with KLRI1 mediates NK cell inhibition whereas heterodimer formation with KLRI2 mediates NK cell activation. Plays a role in allogeneic recognition by the immune system (By similarity). KEYWORDS: Alternative splicing;Cell membrane;Disulfide bond;Glycoprotein;Lectin;Membrane;Receptor;Reference proteome;Signal-anchor;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type II membrane protein " Q80ZD7,"PROTEIN NAMES: Amphoterin-induced protein 1 (AMIGO-1) (Alivin-2) PROTEIN FAMILY: Immunoglobulin superfamily, AMIGO family ORGANISM: Rattus norvegicus (Rat) SIMILARITY: Belongs to the immunoglobulin superfamily. AMIGO family. FUNCTION: Promotes growth and fasciculation of neurites from cultured hippocampal neurons. May be involved in fasciculation as well as myelination of developing neural axons. May have a role in regeneration as well as neural plasticity in the adult nervous system. May mediate homophilic as well as heterophilic cell-cell interaction and contribute to signal transduction through its intracellular domain. Assembled with KCNB1 modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 (By similarity). KEYWORDS: Cell adhesion;Cell membrane;Cell projection;Developmental protein;Differentiation;Disulfide bond;Glycoprotein;Immunoglobulin domain;Leucine-rich repeat;Membrane;Neurogenesis;Phosphoprotein;Reference proteome;Repeat;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein Perikaryon Cell projection, dendrite Cell projection, axon Note=Associated with axons of neuronal cells. Colocalizes with KCNB1 at high-density somatodendritic clusters on the surface of hippocampal and cortical neurons (By similarity). " Q810B8,PROTEIN NAMES: SLIT and NTRK-like protein 4 PROTEIN FAMILY: SLITRK family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the SLITRK family. FUNCTION: It is involved in synaptogenesis and promotes synapse differentiation (By similarity). Suppresses neurite outgrowth. KEYWORDS: Cell membrane;Glycoprotein;Leucine-rich repeat;Membrane;Reference proteome;Repeat;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Single-pass type I membrane protein Cell membrane Q810L3,"PROTEIN NAMES: E3 ubiquitin-protein ligase CHFR (Checkpoint with forkhead and RING finger domains protein) (RING-type E3 ubiquitin transferase CHFR) PROTEIN FAMILY: CHFR family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the CHFR family. FUNCTION: E3 ubiquitin-protein ligase that functions in the antephase checkpoint by actively delaying passage into mitosis in response to microtubule poisons. Acts in early prophase before chromosome condensation, when the centrosome move apart from each other along the periphery of the nucleus. Probably involved in signaling the presence of mitotic stress caused by microtubule poisons by mediating the 'Lys-48'-linked ubiquitination of target proteins, leading to their degradation by the proteasome. Promotes the ubiquitination and subsequent degradation of AURKA and PLK1. Probably acts as a tumor suppressor, possibly by mediating the polyubiquitination of HDAC1, leading to its degradation. May also promote the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2 (UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation, but are rather involved in signaling cellular stress (By similarity). PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: ADP-ribosylation;Alternative splicing;Cell cycle;Cell division;Metal-binding;Mitosis;Nucleus;Phosphoprotein;Reference proteome;Transferase;Ubl conjugation;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus, PML body " Q810S1,"PROTEIN NAMES: Calcium uniporter regulatory subunit MCUb, mitochondrial (MCUb) (Coiled-coil domain-containing protein 109B) PROTEIN FAMILY: MCU family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the MCU (TC 1.A.77) family. FUNCTION: Negatively regulates the activity of MCU, the mitochondrial inner membrane calcium uniporter, and thereby modulates calcium uptake into the mitochondrion. Does not form functional calcium channels by itself. Mitochondrial calcium homeostasis plays key roles in cellular physiology and regulates cell bioenergetics, cytoplasmic calcium signals and activation of cell death pathways. KEYWORDS: Calcium;Calcium transport;Coiled coil;Ion transport;Membrane;Mitochondrion;Mitochondrion inner membrane;Reference proteome;Transit peptide;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Mitochondrion inner membrane ; Multi-pass membrane protein " Q812F8,"PROTEIN NAMES: Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase B (N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase IVb) (GlcNAc-T IVb) (GnT-IVb) (N-acetylglucosaminyltransferase IVb) (UDP-N-acetylglucosamine: alpha-1,3-D-mannoside beta-1,4-N-acetylglucosaminyltransferase IVb) PROTEIN FAMILY: Glycosyltransferase 54 family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the glycosyltransferase 54 family. FUNCTION: Glycosyltransferase that catalyzes the transfer of GlcNAc from UDP-GlcNAc to the GlcNAcbeta1-2Manalpha1-3 arm of the core structure of N-linked glycans through a beta1-4 linkage and participates in the production of tri- and tetra-antennary N-linked sugar chains. Prefers complex-type N-glycans over hybrid-types. Has lower affinities for donors or acceptors than MGAT4A, suggesting that, under physiological conditions, it is not the main contributor in N-glycan biosynthesis. PATHWAY: Protein modification; protein glycosylation. KEYWORDS: Coiled coil;Glycoprotein;Glycosyltransferase;Golgi apparatus;Membrane;Metal-binding;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus membrane ; Single-pass type II membrane protein Note=A processed soluble form also exists. " Q81RQ4,"PROTEIN NAMES: 3-dehydroshikimate dehydratase (3-DHS dehydratase) (DHSase) (Petrobactin biosynthesis protein AsbF) ORGANISM: Bacillus anthracis FUNCTION: Involved in the biosynthesis of petrobactin, a catecholate siderophore that functions in both iron acquisition and virulence. Catalyzes the conversion of 3-dehydroshikimate to 3,4-dihydroxybenzoate (3,4-DHBA). PATHWAY: Aromatic compound metabolism; 3,4-dihydroxybenzoate biosynthesis; 3,4-dihydroxybenzoate from 3-dehydroquinate: step 2/2.; PATHWAY: Siderophore biosynthesis; petrobactin biosynthesis. KEYWORDS: 3D-structure;Lyase;Manganese;Metal-binding;Reference proteome " Q81RQ8,"PROTEIN NAMES: Citryl-spermidine/3,4-dihydroxybenzoyl-citryl-spermidine:spermidine ligase (Petrobactin biosynthesis protein AsbB) PROTEIN FAMILY: IucA/IucC family ORGANISM: Bacillus anthracis SIMILARITY: Belongs to the IucA/IucC family. FUNCTION: Involved in the biosynthesis of petrobactin, a catecholate siderophore that functions in both iron acquisition and virulence. Catalyzes the ATP-dependent condensation of spermidine with N(8)-citryl-spermidine or N(1)-(3,4-dihydroxbenzoyl)-N(8)-citryl-spermidine, two intermediates in petrobactin biosynthesis pathway. PATHWAY: Siderophore biosynthesis; petrobactin biosynthesis. KEYWORDS: 3D-structure;ATP-binding;Ligase;Nucleotide-binding;Reference proteome " Q82IZ1,PROTEIN NAMES: 1-deoxypentalenic acid 11-beta-hydroxylase (Neopentalenolactone biosynthesis protein H) PROTEIN FAMILY: PhyH family ORGANISM: Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL 8165 / MA-4680) SIMILARITY: Belongs to the PhyH family. FUNCTION: Catalyzes the conversion of 1-deoxypentalenic acid to 11-beta-hydroxy-1-deoxypentalenic acid in the biosynthesis of neopentalenolactone antibiotic. PATHWAY: Antibiotic biosynthesis; neopentalenolactone biosynthesis. KEYWORDS: 3D-structure;Antibiotic biosynthesis;Dioxygenase;Iron;Metal-binding;Oxidoreductase;Reference proteome;Vitamin C MISCELLANEOUS: S.avermitilis does not produce pentalenolactone itself in vivo but instead a group of new metabolites that are neopentalenolactone derivatives. Q84HF5,"PROTEIN NAMES: Kynurenine 3-monooxygenase (PfKMO) (Kynurenine 3-hydroxylase) PROTEIN FAMILY: Aromatic-ring hydroxylase family, KMO subfamily ORGANISM: Pseudomonas fluorescens SIMILARITY: Belongs to the aromatic-ring hydroxylase family. KMO subfamily. FUNCTION: Catalyzes the hydroxylation of L-kynurenine (L-Kyn) to form 3-hydroxy-L-kynurenine (L-3OHKyn). Probably required for the synthesis of quinolinic acid and the siderophore quinolobactin. PATHWAY: Cofactor biosynthesis; NAD(+) biosynthesis; quinolinate from L-kynurenine: step 1/3.; PATHWAY: Siderophore biosynthesis; quinolobactin biosynthesis. KEYWORDS: 3D-structure;FAD;Flavoprotein;Monooxygenase;NADP;Oxidoreductase;Pyridine nucleotide biosynthesis " Q84JE8,"PROTEIN NAMES: Polynucleotide 3'-phosphatase ZDP (DNA nick sensor protein) PROTEIN FAMILY: DNA 3' phosphatase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: In the C-terminal section; belongs to the DNA 3' phosphatase family. FUNCTION: Nick-sensing 3'-phosphoesterase involved in a base excision repair pathway required for active DNA demethylation. The N-terminal DNA-binding domain binds specifically to gap sites and sharply bends the target DNA. Lacks 5'-kinase activity but is capable of 3'-phosphoglycolate end processing. Inactive on 3'-alpha,beta-unsaturated aldehyde (3'-dRP). Protects partially genes from transcriptional silencing by preventing promoter DNA hypermethylation. KEYWORDS: Alternative splicing;DNA damage;DNA repair;DNA-binding;Hydrolase;Metal-binding;Nucleus;Reference proteome;Repeat;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus, nucleoplasm Note=Not found in the nucleolus. Co-localizes in nucleoplasmic foci with APE1L and ROS1, two components of the DNA demethylase machinery. " Q84JG2,"PROTEIN NAMES: SWI/SNF complex subunit SWI3B (AtSWI3B) (Transcription regulatory protein SWI3B) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Component of a multiprotein complex equivalent of the SWI/SNF complex, an ATP-dependent chromatin-remodeling complex, which is required for the positive and negative regulation of gene expression of a large number of genes. It changes chromatin structure by altering DNA-histone contacts within a nucleosome, leading eventually to a change in nucleosome position, thus facilitating or repressing binding of gene-specific transcription factors. May play an essential role in the transition from the vegetative to the reproductive phase of development. May be a positive regulator of ABA signaling. KEYWORDS: Activator;Chromatin regulator;Coiled coil;Developmental protein;DNA-binding;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus " Q84JS6,"PROTEIN NAMES: Homeobox protein knotted-1-like 6 (Protein KNAT6) PROTEIN FAMILY: TALE/KNOX homeobox family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the TALE/KNOX homeobox family. FUNCTION: Plays a role in meristem function. Contributes to the shoot apical meristem (SAM) maintenance and organ separation by controlling boundary establishment in embryo in a CUC1, CUC2 and STM-dependent manner. Involved in maintaining cells in an undifferentiated, meristematic state. Probably binds to the DNA sequence 5'-TGAC-3'. KEYWORDS: Alternative splicing;DNA-binding;Homeobox;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus " Q84JZ6,"PROTEIN NAMES: Multiple organellar RNA editing factor 3, mitochondrial (RNA editing-interacting protein 3) PROTEIN FAMILY: MORF family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the MORF family. FUNCTION: Involved in organellar RNA editing. Required for the processing of RNA editing sites in mitochondria. KEYWORDS: Alternative splicing;Mitochondrion;mRNA processing;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Mitochondrion " Q84K00,"PROTEIN NAMES: NAC domain-containing protein 78 (ANAC078) (Protein NTM1-like 11) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Transcriptional activator activated by proteolytic cleavage through regulated intramembrane proteolysis (RIP) (By similarity). Transcripition activator associated with the induction of genes related to flavonoid biosynthesis and required for the accumulation of anthocyanins in response to high light stress. Plays a role in the regulation of 20S and 26S proteasomes in response to high light stress. KEYWORDS: Activator;DNA-binding;Membrane;Nucleus;Reference proteome;Stress response;Transcription;Transcription regulation;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Single-pass membrane protein Nucleus Note=Localized primarily in plasma membrane or endoplasmic reticulum membrane as dormant form and, upon specific stress or signal, is processed into a transcriptionally active and nuclear form after a proteolytic cleavage through regulated intramembrane proteolysis (RIP). " Q84MA2,"PROTEIN NAMES: Type I inositol polyphosphate 5-phosphatase 1 (At5PTase1) PROTEIN FAMILY: Inositol polyphosphate 5-phosphatase family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the inositol polyphosphate 5-phosphatase family. FUNCTION: Has phosphatase activity toward Ins(1,4,5)P3 and Ins(1,3,4,5)P4, but not toward Ins(1,4)P2, Ins(1)P. Seems to be involved in the abscisic acid (ABA) signaling pathway. Could also be able to hydrolyze PtdIns(4,5)P2 and PtdIns(3,4,5)P3. KEYWORDS: Abscisic acid signaling pathway;Alternative splicing;Hydrolase;Phosphoprotein;Reference proteome MISCELLANEOUS: [Isoform 2]: May be due to a donor acceptor splice site." Q84MB5,"PROTEIN NAMES: Rhomboid-like protein 11, chloroplastic (AtRBL11) PROTEIN FAMILY: Peptidase S54 family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the peptidase S54 family. FUNCTION: Rhomboid-type serine protease that catalyzes intramembrane proteolysis. May be involved in TIC22 processing during its import. KEYWORDS: Chloroplast;Hydrolase;Membrane;Plastid;Plastid inner membrane;Protease;Reference proteome;Transit peptide;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Plastid, chloroplast inner membrane ; Multi-pass membrane protein " Q84ME1,"PROTEIN NAMES: Probable E3 ubiquitin-protein ligase LUL3 (Probable RING-type E3 ubiquitin transferase LUL3) (Protein LOG2-LIKE UBIQUITIN LIGASE 3) (RING finger protein 398) PROTEIN FAMILY: RING-type zinc finger family, LOG2 subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the RING-type zinc finger family. LOG2 subfamily. FUNCTION: Acts as an E3 ubiquitin-protein ligase, or as part of E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes and then transfers it to substrates (in vitro). PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Lipoprotein;Metal-binding;Myristate;Reference proteome;Transferase;Ubl conjugation pathway;Zinc;Zinc-finger " Q84N64,"PROTEIN NAMES: Dynamin-like protein ARC5 (Dynamin-related protein 5B) (Protein ACCUMULATION AND REPLICATION OF CHLOROPLASTS 5) (AtARC5) PROTEIN FAMILY: TRAFAC class dynamin-like GTPase superfamily, Dynamin/Fzo/YdjA family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family. FUNCTION: Mechanochemical GTPase component of both plastid and peroxisome division machinery. Required for the last steps of plastid division specifically in mesophyll-cell, when the narrow isthmus breaks, facilitating the separation of the daughter plastids. Necessary for peroxisome activities. Seems to influence stromule (stroma-filled tubular extensions of the plastid envelope membrane) length and frequency. KEYWORDS: Alternative splicing;Chloroplast;Coiled coil;Cytoplasm;GTP-binding;Hydrolase;Membrane;Motor protein;Nucleotide-binding;Peroxisome;Peroxisome biogenesis;Plastid;Plastid outer membrane;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Plastid, chloroplast outer membrane ; Peripheral membrane protein Peroxisome Cytoplasm, cytosol Note=Plastid equatorial positioning (in a discontinuous ring shape) mediated by PDV2 in complex with ARC6 and dissociation from the outer envelope membranes (OEMs) is triggered by PDV1; this localization with OEMs is repressed by phosphatidylinositol 4-phosphate (PI4P). Dynamic subunit exchange within chloroplastic DRP5B rings with a cytosolic pool during chloroplast membrane constriction in a GTPase activity-dependent manner. " Q84TH5,"PROTEIN NAMES: ABC transporter G family member 25 (ABC transporter ABCG.25) (AtABCG25) (White-brown complex homolog protein 26) (AtWBC26) PROTEIN FAMILY: ABC transporter superfamily, ABCG family, Eye pigment precursor importer subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily. FUNCTION: High affinity abscisic acid (ABA) transporter that mediates the export of ABA, with a preference for (+)-ABA, through the plasma membrane, especially in vascular tissues (e.g. phloem companion cells), and is involved in the intercellular ABA signaling pathway. Together with ABCG31, export ABA from the endosperm to deliver it to the embryo via ABCG30 and ABCG40-mediated import to suppress radicle extension and subsequent embryonic growth. KEYWORDS: 3D-structure;Abscisic acid signaling pathway;Alternative splicing;ATP-binding;Cell membrane;Glycoprotein;Membrane;Nucleotide-binding;Reference proteome;Stress response;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein " Q84TH9,PROTEIN NAMES: Protein NLP7 (AtNLP7) (NIN-like protein 7) (Nodule inception protein-like protein 7) ORGANISM: Arabidopsis thaliana (Mouse-ear cress) FUNCTION: Transcription factor involved in regulation of nitrate assimilation and in transduction of the nitrate signal. KEYWORDS: Coiled coil;DNA-binding;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus Q84V22,"PROTEIN NAMES: Phosphatidylserine decarboxylase proenzyme 1, mitochondrial [Cleaved into: Phosphatidylserine decarboxylase 1 beta chain; Phosphatidylserine decarboxylase 1 alpha chain] PROTEIN FAMILY: Phosphatidylserine decarboxylase family, PSD-B subfamily, Eukaryotic type I sub-subfamily ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the phosphatidylserine decarboxylase family. PSD-B subfamily. Eukaryotic type I sub-subfamily. FUNCTION: Catalyzes the formation of phosphatidylethanolamine (PtdEtn) from phosphatidylserine (PtdSer). Plays a central role in phospholipid metabolism and in the interorganelle trafficking of phosphatidylserine. Contributes only to a minor proportion of PtdEtn production. PATHWAY: Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from CDP-diacylglycerol: step 2/2. KEYWORDS: Decarboxylase;Lipid biosynthesis;Lipid metabolism;Lyase;Membrane;Mitochondrion;Mitochondrion inner membrane;Phospholipid biosynthesis;Phospholipid metabolism;Pyruvate;Reference proteome;Transit peptide;Transmembrane;Transmembrane helix;Zymogen SUBCELLULAR LOCATION: [Phosphatidylserine decarboxylase 1 beta chain]: Mitochondrion Mitochondrion inner membrane ; Single-pass membrane protein ; Intermembrane side.; SUBCELLULAR LOCATION: [Phosphatidylserine decarboxylase 1 alpha chain]: Mitochondrion Mitochondrion inner membrane ; Peripheral membrane protein ; Intermembrane side Note=Anchored to the mitochondrial inner membrane through its interaction with the integral membrane beta chain. " Q84W66,PROTEIN NAMES: Nuclear transcription factor Y subunit B-6 (AtNF-YB-6) (AtNF-YB6) (Protein LEAFY COTYLEDON 1-LIKE) PROTEIN FAMILY: NFYB/HAP3 subunit family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the NFYB/HAP3 subunit family. FUNCTION: Component of the NF-Y/HAP transcription factor complex. The NF-Y complex stimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters. Plays a role in the regulation of the embryogenesis. Involved in the abscisic acid (ABA) signaling pathway. KEYWORDS: 3D-structure;Abscisic acid signaling pathway;Activator;Alternative splicing;DNA-binding;Nucleus;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus Q84WV6,"PROTEIN NAMES: Protein MICRORCHIDIA 1 (AtMORC1) (Protein COMPROMISED RECOGNITION OF TCV 1) PROTEIN FAMILY: MORC ATPase protein family ORGANISM: Arabidopsis thaliana (Mouse-ear cress) SIMILARITY: Belongs to the MORC ATPase protein family. FUNCTION: Mediator of defense signaling triggered by distinct classes of R proteins. Required during hypersensitive response (HR) that confers disease resistance to turnip crinkle virus (TCV). Exhibits ATPase activity. Contributes to resistance against Pseudomonas syringae and Hyaloperonospora arabidopsidis, at early stages prior to cytosolic calcium ions Ca(2+) accumulation. Required for pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI), basal resistance, non-host resistance and systemic acquired resistance (SAR). Binds DNA/RNA in a non-specific manner and exhibits endonuclease activity. Probably involved in DNA repair. Required for both RPP8- and SSI4-mediated resistance responses, thus being involved in both TIR- and CC-NB-LRR pathways. Involved in RNA-directed DNA methylation (RdDM) as a component of the RdDM machinery and required for gene silencing. May also be involved in the regulation of chromatin architecture to maintain gene silencing. KEYWORDS: ATP-binding;Chromatin regulator;Coiled coil;DNA damage;DNA repair;DNA-binding;Endonuclease;Endosome;Hydrolase;Hypersensitive response;Nuclease;Nucleotide-binding;Nucleus;Plant defense;Reference proteome;RNA-binding;RNA-mediated gene silencing SUBCELLULAR LOCATION: Nucleus Endosome Note=Present in nuclear bodies near chromocenters. Localized in endosome-like vesicles displaying rapid cytosolic streaming. Accumulates in the nucleus following pathogen-associated molecular pattern (PAMP) treatment or infection with an avirulent pathogen. " Q852L0,"PROTEIN NAMES: Casein kinase 1-like protein HD16 (Os03g0793500 protein) (Protein EARLY FLOWERING 1) (Protein HEADING DATE 16) PROTEIN FAMILY: Protein kinase superfamily, CK1 Ser/Thr protein kinase family, Casein kinase I subfamily ORGANISM: Oryza sativa subsp. japonica (Rice) SIMILARITY: Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily. FUNCTION: Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins (Probable). Can phosphorylate casein on threonine residues in vitro. Involved in the regulation of flowering time through gibberellin (GA) signaling, and independently of photoperiod. Phosphorylates the DELLA protein SLR1, stabilizing SLR1 protein and sustaining SLR1 activity as repressor of GA signaling. Required for normal development of male floral organs and grains, through modulation of GA signaling. Targeted and repressed by the homeobox protein HAZ1 during GA signaling. Can phosphorylate phosvitin and SLR1 in vitro. Is not required for clock function in either the presence or the absence of light signals. Involved in a genetic control pathway for photoperiodic flowering under long day (LD) conditions that includes HD1, GHD7, HD5 and HD2. Phosphorylates and activates GHD7, a major floral repressor under LD conditions. Phosphorylation of GHD7 enhances its function in the repression of EHD1, HD3A and HD3B/RFT1, and obviously delaying flowering. KEYWORDS: ATP-binding;Cytoplasm;Flowering;Gibberellin signaling pathway;Growth regulation;Kinase;Nucleotide-binding;Nucleus;Reference proteome;Serine/threonine-protein kinase;Transferase SUBCELLULAR LOCATION: Cytoplasm Nucleus " Q868G6,"PROTEIN NAMES: Tachykinins (AmTRP) (Tachykinin-related peptide) [Cleaved into: APTGHQEMQ-amide (AmTRP1) (Tachykinin-related peptide 1) (TRP1); ALMGFQGVR-amide (AmTRP2) (Tachykinin-related peptide 2) (TRP2); Brain peptide NSIINDVKNELFPEDIN; APMGFQGMR-amide 1 (AmTRP3) (Tachykinin-related peptide 3-1) (TRP3-1); Brain peptide ASFDDEYY; APMGFQGMR-amide 2 (AmTRP3) (Tachykinin-related peptide 3-2) (TRP3-2); Brain peptide SLEEILDEIK; Brain peptide SLEEILDEI; Brain peptide EILDEI; TTRFQDSRSKDVYLIDYPEDY-amide (AmTAP1) (Tachykinin-associated peptide 1); Brain peptide VLSMDGYQNILDKKDELLGEWE; Brain peptide APMGFYGT; APMGFYGTR-amide (AmTRP4) (Tachykinin-related peptide 4) (TRP4); Brain peptide IILDALEELD; Brain peptide ILDALEELD; Brain peptide GVMDFQIGLQ; ARMGFHGMR-amide (AmTRP5) (Tachykinin-related peptide 5) (TRP5); SPFRYLGAR-amide (AmTRP6) (Tachykinin-related peptide 6) (TRP6); NPRWEFRGKFVGVR-amide (AmTRP7) (Tachykinin-related peptide 7) (TRP7)] PROTEIN FAMILY: Tachykinin family ORGANISM: Apis mellifera (Honeybee) SIMILARITY: Belongs to the tachykinin family. FUNCTION: Tachykinins are active peptides which excite neurons, evoke behavioral responses, are potent vasodilators and secretagogues, and contract (directly or indirectly) many smooth muscles. Associated with sex-specific or age/division of labor-selective behavior and/or physiology of honeybees. KEYWORDS: Amidation;Behavior;Cleavage on pair of basic residues;Direct protein sequencing;Neuropeptide;Neurotransmitter;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted " Q86B61,"PROTEIN NAMES: Tyramine beta-hydroxylase (Tyramine beta-monooxygenase) (TbetaM) PROTEIN FAMILY: Copper type II ascorbate-dependent monooxygenase family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the copper type II ascorbate-dependent monooxygenase family. FUNCTION: Catalyzes the hydroxylation of tyramine into octopamine, a neurotransmitter involved in ovulation and locomotion. Functions in an amine-mediated Bacc-dependent signaling pathway that negatively regulates acute ethanol sensitivity. Involved in facilitation of nociceptive escape behavior in response to potentially damaging stimuli, such as high temperatures. KEYWORDS: Copper;Disulfide bond;Glycoprotein;Membrane;Metal-binding;Monooxygenase;Neurotransmitter biosynthesis;Oxidoreductase;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Single-pass membrane protein " Q86B79,PROTEIN NAMES: RING finger protein unkempt PROTEIN FAMILY: Unkempt family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the unkempt family. FUNCTION: Essential for late larval/early pupal development. KEYWORDS: Cytoplasm;Developmental protein;DNA-binding;Metal-binding;Phosphoprotein;Reference proteome;Repeat;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cytoplasm Q86C56,PROTEIN NAMES: Soluble guanylate cyclase gcy-31 PROTEIN FAMILY: Adenylyl cyclase class-4/guanylyl cyclase family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the adenylyl cyclase class-4/guanylyl cyclase family. FUNCTION: Synthesizes cyclic GMP (cGMP) from GTP. May play a role in embryogenesis. KEYWORDS: Alternative splicing;cGMP biosynthesis;Coiled coil;Cytoplasm;GTP-binding;Heme;Iron;Lyase;Magnesium;Metal-binding;Nucleotide-binding;Reference proteome SUBCELLULAR LOCATION: Cytoplasm MISCELLANEOUS: There are two types of guanylate cyclases: soluble forms and membrane-associated receptor forms. Q86KR9,PROTEIN NAMES: Prolyl 4-hydroxylase subunit alpha (Prolyl 4-hydrolase) PROTEIN FAMILY: P4HA family ORGANISM: Dictyostelium discoideum (Social amoeba) SIMILARITY: Belongs to the P4HA family. FUNCTION: Catalyzes the post-translational formation of 4-hydroxyproline. Probably hydroxylates skp1 on Pro-143. KEYWORDS: 3D-structure;Cytoplasm;Dioxygenase;Iron;Metal-binding;Oxidoreductase;Reference proteome;Vitamin C SUBCELLULAR LOCATION: Cytoplasm Q86L04,"PROTEIN NAMES: TNF receptor-associated protein 1 homolog, mitochondrial (TNFR-associated protein 1 homolog) (Trap1 homolog) PROTEIN FAMILY: Heat shock protein 90 family ORGANISM: Dictyostelium discoideum (Social amoeba) SIMILARITY: Belongs to the heat shock protein 90 family. FUNCTION: Chaperone that expresses an ATPase activity. KEYWORDS: ATP-binding;Chaperone;Cytoplasm;Cytoskeleton;Mitochondrion;Nucleotide-binding;Nucleus;Reference proteome;Stress response;Transit peptide SUBCELLULAR LOCATION: Cytoplasm, cell cortex. Cytoplasm, cytoskeleton. Mitochondrion. Spore, perispore. Nucleus, nucleolus. Note=Localizes to the cortical actin cytoskeleton. Translocates to the mitochondrion during the prestarvation response and in response to differentiation. In prespore cells, colocalizes with grp94 in the prespore-specific vacuole. Found in the outermost layer of spore cell wall. " Q86MA7,PROTEIN NAMES: Protein PRQFV-amide [Cleaved into: AREFV-amide; PRQFV-amide (PRQFVa); VRDFV-amide; VREFV-amide; IREFV-amide] ORGANISM: Aplysia californica (California sea hare) FUNCTION: PRQFV-amide may act as a modulator within the feeding system as well as in other systems of Aplysia. KEYWORDS: Amidation;Neuropeptide;Secreted;Signal SUBCELLULAR LOCATION: Secreted. Q86MI0,"PROTEIN NAMES: Homeobox protein dve-1 (Defective proventriculus homolog protein) ORGANISM: Caenorhabditis elegans FUNCTION: Transcription factor. Acts during mitochondrial stress by activating the mitochondrial unfolded protein response (mtUPR). Required during mitochondrial stress for the activation of genes involved in the mtUPR, in concert with histone deacetylase hda-1. In response to the citrate-induced mtUPR, positively regulates transcription of transcription factor nhr-80, thereby leading to modulation of lipid metabolism. May play a role in modulating the decline in protein homeostasis associated with normal aging. Required for embryonic development and maintenance of mitochondrial morphology. KEYWORDS: Alternative splicing;Cytoplasm;DNA-binding;Homeobox;Nucleus;Reference proteome;Repeat;Ubl conjugation SUBCELLULAR LOCATION: Nucleus Cytoplasm, cytosol Note=Upon mitochondrial stress translocates from cytosol into the nucleus. " Q86MP3,"PROTEIN NAMES: Ectopic P granules protein 6 PROTEIN FAMILY: WD repeat PROPPIN family ORGANISM: Caenorhabditis elegans SIMILARITY: Belongs to the WD repeat PROPPIN family. FUNCTION: Component of the epg-6/atg-2 complex, which is involved in the generation of autophagosomes from omegasomes and in the distribution of atg-9 and atg-13 during the autophagy-mediated degradation of protein aggregates. Binds to phosphatidylinositols on preautophagosomes, which are early autophagic structures, to promote autophagosome formation. In particular, binds with high affinity to phosphatidylinositols including phosphatidylinositol 3-phosphate (PtdIns(3)P) and phosphatidylinositol 5-phosphate (PtdIns(5)P), but more weakly to phosphatidylinositol 4-phosphate (PtdIns(4)P) and phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Involved in autophagy-mediated degradation of ribosomal RNA and ribosomal proteins in lysosomes, which is essential for maintaining nucleotide homeostasis. KEYWORDS: Alternative splicing;Autophagy;Cytoplasm;Lipid-binding;Membrane;Reference proteome;Repeat;WD repeat SUBCELLULAR LOCATION: Cytoplasm Preautophagosomal structure membrane ; Peripheral membrane protein Note=Localizes to the cytoplasm of all cells during embryogenesis. " Q86MV5,"PROTEIN NAMES: Terminal uridylyltransferase 2 (TUTase 2) (Mitochondrial protein 57) (TbMP57) (RNA editing 3' terminal uridylyltransferase 2) (RET2) (RNA editing TUTase 2) (RNA editing complex protein MP57) PROTEIN FAMILY: DNA polymerase type-B-like family ORGANISM: Trypanosoma brucei brucei SIMILARITY: Belongs to the DNA polymerase type-B-like family. FUNCTION: Terminal uridylyltransferase which, as part of the mitochondrial RNA editing core complex (RECC), is involved in the post-transcriptional editing of mitochondrial RNA, a process involving the addition and deletion of uridine (U) nucleotides in the pre-mRNA. Specifically, catalyzes the addition of one U to single-stranded RNA with a preference for a 3'-terminal A or G and adds the number of Us specified by a guide RNA (gRNA) to precleaved double-stranded RNA editing substrates. Essential for the survival of the bloodstream form. KEYWORDS: 3D-structure;Magnesium;Manganese;Metal-binding;Mitochondrion;mRNA processing;Nucleotide-binding;Nucleotidyltransferase;RNA-binding;Transferase;Transit peptide SUBCELLULAR LOCATION: Mitochondrion " Q86PC9,"PROTEIN NAMES: Protein king tubby PROTEIN FAMILY: TUB family ORGANISM: Drosophila melanogaster (Fruit fly) SIMILARITY: Belongs to the TUB family. FUNCTION: Functions in regulating protein trafficking, retinal maintenance and lipid storage. Protects photoreceptor cells R1 to R6 against light-induced retinal degeneration by stimulating norpA-mediated endocytosis of the rhodopsin ninaE (Rh1). In the auditory receptor neurons, functions as a cilia trafficking regulator of various transient receptor potential (TRP) channel components including iav and nompC. Likely to deliver pre-ciliary vesicles containing membrane proteins such as iav and nompC to the intraflagellar transport complex (IFT) at the cilia base. Plays a role in the inhibition of fat storage. KEYWORDS: Alternative splicing;Cell membrane;Cell projection;Cytoplasm;Lipid-binding;Membrane;Nucleus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Nucleus Cell projection, cilium membrane ; Peripheral membrane protein Cell projection, rhabdomere Note=Detected in the cytoplasm and nucleus of the chordotonal neurons. PtdIns 4,5-P2 binds to and regulates its localization to the ciliary base. In the dark, localizes mainly to the rhabdomere domain of photoreceptor cells R1 to R6. When dark-reared flies are moved into the light, the protein translocates to the cytoplasm. Under normal conditions (12 hr light/12 hr dark), localized mainly in the nucleus of photoreceptor cells with minor staining in the cytoplasm. " Q86T13,PROTEIN NAMES: C-type lectin domain family 14 member A (Epidermal growth factor receptor 5) (EGFR-5) ORGANISM: Homo sapiens (Human) KEYWORDS: Direct protein sequencing;Disulfide bond;EGF-like domain;Glycoprotein;Lectin;Membrane;Reference proteome;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane ; Single-pass type I membrane protein Q86TL2,"PROTEIN NAMES: Store-operated calcium entry regulator STIMATE (STIM-activating enhancer encoded by TMEM110) (Transmembrane protein 110) PROTEIN FAMILY: STIMATE family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the STIMATE family. FUNCTION: Acts as a regulator of store-operated Ca(2+) entry (SOCE) at junctional sites that connect the endoplasmic reticulum (ER) and plasma membrane (PM), called ER-plasma membrane (ER-PM) junction or cortical ER. SOCE is a Ca(2+) influx following depletion of intracellular Ca(2+) stores. Acts by interacting with STIM1, promoting STIM1 conformational switch. Involved in STIM1 relocalization to ER-PM junctions. Contributes to the maintenance and reorganization of store-dependent ER-PM junctions. KEYWORDS: Endoplasmic reticulum;Membrane;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein Note=Colocalizes with STIM1 at ER-plasma membrane (ER-PM) junctions, also called cortical endoplasmic reticulum (ER), in store-depleted calcium cells. May translocate to ER-PM junctions in a STIM1-dependent manner in store-depleted cells (Probable). " Q86UD4,PROTEIN NAMES: Zinc finger protein 329 PROTEIN FAMILY: Krueppel C2H2-type zinc-finger protein family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein family. FUNCTION: May be involved in transcriptional regulation. KEYWORDS: DNA-binding;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Repeat;Transcription;Transcription regulation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Q86UP0,PROTEIN NAMES: Cadherin-24 ORGANISM: Homo sapiens (Human) FUNCTION: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Cadherin-24 mediate strong cell-cell adhesion. KEYWORDS: Alternative splicing;Calcium;Cell adhesion;Cell membrane;Cleavage on pair of basic residues;Glycoprotein;Membrane;Metal-binding;Reference proteome;Repeat;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein Q86VI4,"PROTEIN NAMES: Lysosomal-associated transmembrane protein 4B (Lysosome-associated transmembrane protein 4-beta) PROTEIN FAMILY: LAPTM4/LAPTM5 transporter family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the LAPTM4/LAPTM5 transporter family. FUNCTION: Required for optimal lysosomal function. Blocks EGF-stimulated EGFR intraluminal sorting and degradation. Conversely by binding with the phosphatidylinositol 4,5-bisphosphate, regulates its PIP5K1C interaction, inhibits HGS ubiquitination and relieves LAPTM4B inhibition of EGFR degradation. Recruits SLC3A2 and SLC7A5 (the Leu transporter) to the lysosome, promoting entry of leucine and other essential amino acid (EAA) into the lysosome, stimulating activation of proton-transporting vacuolar (V)-ATPase protein pump (V-ATPase) and hence mTORC1 activation. Plays a role as negative regulator of TGFB1 production in regulatory T cells. Binds ceramide and facilitates its exit from late endosome in order to control cell death pathways. KEYWORDS: Alternative splicing;Cell membrane;Cell projection;Endosome;Lysosome;Membrane;Reference proteome;Transmembrane;Transmembrane helix;Transport;Ubl conjugation SUBCELLULAR LOCATION: Endomembrane system ; Multi-pass membrane protein Late endosome membrane Cell membrane Cell projection Lysosome membrane Endosome membrane. Endosome, multivesicular body membrane Endosome, multivesicular body lumen " Q86VK4,"PROTEIN NAMES: Zinc finger protein 410 (Another partner for ARF 1) ORGANISM: Homo sapiens (Human) FUNCTION: Transcription factor that binds to the sequence motif 5'-CATCCCATAATA-3', and is specifically required to silence expression of fetal hemoglobin in adult erythroid cells. Prevents expression of fetal hemoglobin genes HBG1 and HBG2 through CHD4: acts as a direct transcriptional activator of CHD4, a central component of the NuRD complex that represses transcription of fetal hemoglobin genes HBG1 and HBG2 in erythroid cells. May also activate transcription of matrix-remodeling genes such as MMP1 during fibroblast senescence. May activate transcription of the gap junction gene GJC1, perhaps in response to increasing glucose. However, recent studies suggest that ZNF410 is dedicated to regulate expression of a single gene: CHD4. KEYWORDS: 3D-structure;Activator;Alternative splicing;Chromosome;DNA-binding;Glycoprotein;Metal-binding;Nucleus;Reference proteome;Repeat;Transcription;Transcription regulation;Ubl conjugation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Chromosome Note=Directly binds to the sequence motif 5'-CATCCCATAATA-3'. MISCELLANEOUS: [Isoform 2]: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay." Q86VQ3,"PROTEIN NAMES: Thioredoxin domain-containing protein 2 (Spermatid-specific thioredoxin-1) (Sptrx-1) ORGANISM: Homo sapiens (Human) FUNCTION: Probably plays a regulatory role in sperm development. May participate in regulation of fibrous sheath (FS) assembly by supporting the formation of disulfide bonds during sperm tail morphogenesis. May also be required to rectify incorrect disulfide pairing and generate suitable pairs between the FS constituents. Can reduce disulfide bonds in vitro in the presence of NADP and thioredoxin reductase. KEYWORDS: Alternative splicing;Cytoplasm;Developmental protein;Differentiation;Disulfide bond;Phosphoprotein;Redox-active center;Reference proteome;Repeat;Spermatogenesis SUBCELLULAR LOCATION: Cytoplasm Note=In ejaculated spermatozoa, it localizes in the caudal region of the head to the end of the principal piece. " Q86VX9,"PROTEIN NAMES: Vacuolar fusion protein MON1 homolog A PROTEIN FAMILY: MON1/SAND family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the MON1/SAND family. FUNCTION: Plays an important role in membrane trafficking through the secretory apparatus. Not involved in endocytic trafficking to lysosomes (By similarity). Acts in concert with CCZ1, as a guanine exchange factor (GEF) for RAB7, promotes the exchange of GDP to GTP, converting it from an inactive GDP-bound form into an active GTP-bound form. KEYWORDS: Alternative splicing;Guanine-nucleotide releasing factor;Phosphoprotein;Reference proteome " Q86VY4,PROTEIN NAMES: Testis-specific Y-encoded-like protein 5 (TSPY-like protein 5) PROTEIN FAMILY: Nucleosome assembly protein (NAP) family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the nucleosome assembly protein (NAP) family. FUNCTION: Involved in modulation of cell growth and cellular response to gamma radiation probably via regulation of the Akt signaling pathway. Involved in regulation of p53/TP53. Suppresses p53/TP53 protein levels and promotes its ubiquitination; the function is dependent on USP7 and independent on MDM2. Proposed to displace p53/TP53 from interaction with USP7. KEYWORDS: Growth regulation;Reference proteome Q86W47,"PROTEIN NAMES: Calcium-activated potassium channel subunit beta-4 (BK channel subunit beta-4) (BKbeta4) (Hbeta4) (Calcium-activated potassium channel, subfamily M subunit beta-4) (Charybdotoxin receptor subunit beta-4) (K(VCA)beta-4) (Maxi K channel subunit beta-4) (Slo-beta-4) PROTEIN FAMILY: KCNMB family, KCNMB4 subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the KCNMB (TC 8.A.14.1) family. KCNMB4 subfamily. FUNCTION: Regulatory subunit of the calcium activated potassium KCNMA1 (maxiK) channel. Modulates the calcium sensitivity and gating kinetics of KCNMA1, thereby contributing to KCNMA1 channel diversity. Decreases the gating kinetics and calcium sensitivity of the KCNMA1 channel, but with fast deactivation kinetics. May decrease KCNMA1 channel openings at low calcium concentrations but increases channel openings at high calcium concentrations. Makes KCNMA1 channel resistant to 100 nM charybdotoxin (CTX) toxin concentrations. KEYWORDS: 3D-structure;Glycoprotein;Ion channel;Ion transport;Membrane;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. MISCELLANEOUS: Treatment with okadaic acid reduces its effect on KCNMA1." Q86WA6,"PROTEIN NAMES: Valacyclovir hydrolase (VACVase) (Valacyclovirase) (Biphenyl hydrolase-like protein) (Biphenyl hydrolase-related protein) (Bph-rp) (Breast epithelial mucin-associated antigen) (MCNAA) PROTEIN FAMILY: AB hydrolase superfamily, Lipase family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the AB hydrolase superfamily. Lipase family. FUNCTION: Serine hydrolase that catalyzes the hydrolytic activation of amino acid ester prodrugs of nucleoside analogs such as valacyclovir and valganciclovir. Activates valacyclovir to acyclovir. May play a role in detoxification processes. It is a specific alpha-amino acid ester hydrolase that prefers small, hydrophobic, and aromatic side chains and does not have a stringent requirement for the leaving group other than preferring a primary alcohol. KEYWORDS: 3D-structure;Acetylation;Alternative splicing;Direct protein sequencing;Hydrolase;Reference proteome;Signal " Q86WS5,"PROTEIN NAMES: Transmembrane protease serine 12 PROTEIN FAMILY: Peptidase S1 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the peptidase S1 family. FUNCTION: Required for male fertility (By similarity). Plays a critical role in sperm capacitation and acrosome reactions during fertilization, and also plays a role in the regulation of proteins involved in spermatogenesis (By similarity). Regulates protein pathways that promote chromosomal synapsis formation, double-strand break repair, formation of the inner mitochondrial membrane cristae and apoptosis in developing sperm (By similarity). Required for normal sperm motility and binding to the zona pellucida, potentially via a role in ADAM3 protein maturation (By similarity). KEYWORDS: Cell membrane;Cytoplasmic vesicle;Differentiation;Disulfide bond;Fertilization;Glycoprotein;Hydrolase;Membrane;Protease;Reference proteome;Serine protease;Signal;Spermatogenesis;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass membrane protein Cytoplasmic vesicle, secretory vesicle, acrosome Note=Expression in the acrosome decreases after acrosome reaction. MISCELLANEOUS: Expressed in colorectal cancer (at protein level)." Q86WU2,"PROTEIN NAMES: Probable D-lactate dehydrogenase, mitochondrial (DLD) (Lactate dehydrogenase D) PROTEIN FAMILY: FAD-binding oxidoreductase/transferase type 4 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the FAD-binding oxidoreductase/transferase type 4 family. FUNCTION: Involved in D-lactate, but not L-lactate catabolic process. KEYWORDS: Acetylation;Alternative splicing;Disease variant;FAD;Flavoprotein;Mitochondrion;Oxidoreductase;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Mitochondrion " Q86X29,"PROTEIN NAMES: Lipolysis-stimulated lipoprotein receptor (Angulin-1) PROTEIN FAMILY: Immunoglobulin superfamily, LISCH7 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the immunoglobulin superfamily. LISCH7 family. FUNCTION: Probable role in the clearance of triglyceride-rich lipoprotein from blood. Binds chylomicrons, LDL and VLDL in presence of free fatty acids and allows their subsequent uptake in the cells (By similarity). Maintains epithelial barrier function by recruiting MARVELD2/tricellulin to tricellular tight junctions (By similarity). KEYWORDS: Alternative splicing;Cell junction;Cell membrane;Chylomicron;Disulfide bond;Immunoglobulin domain;LDL;Membrane;Phosphoprotein;Receptor;Reference proteome;Tight junction;Transmembrane;Transmembrane helix;VLDL SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein Cell junction, tight junction Note=Located at tricellular contacts. " Q86XJ1,"PROTEIN NAMES: GAS2-like protein 3 (Growth arrest-specific protein 2-like 3) PROTEIN FAMILY: GAS2 family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the GAS2 family. FUNCTION: Cytoskeletal linker protein. May promote and stabilize the formation of the actin and microtubule network. KEYWORDS: Actin-binding;Cytoplasm;Cytoskeleton;Microtubule;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Cytoplasm, cytoskeleton Note=Localizes to microtubule and actin cytoskeletons. " Q86XN8,"PROTEIN NAMES: RNA-binding protein MEX3D (RING finger and KH domain-containing protein 1) (RING finger protein 193) (TINO) ORGANISM: Homo sapiens (Human) FUNCTION: RNA binding protein, may be involved in post-transcriptional regulatory mechanisms. KEYWORDS: 3D-structure;Alternative splicing;Cytoplasm;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Repeat;RNA-binding;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cytoplasm Nucleus Note=Predominantly expressed in the cytoplasm and shuttles between the cytoplasm and the nucleus through the CRM1 export pathway. " Q86XR7,"PROTEIN NAMES: TIR domain-containing adapter molecule 2 (TICAM-2) (Putative NF-kappa-B-activating protein 502) (TRIF-related adapter molecule) (Toll-like receptor adaptor protein 3) (Toll/interleukin-1 receptor domain-containing protein) (MyD88-4) ORGANISM: Homo sapiens (Human) FUNCTION: Functions as a sorting adapter in different signaling pathways to facilitate downstream signaling leading to type I interferon induction. In TLR4 signaling, physically bridges TLR4 and TICAM1 and functionally transmits signal to TICAM1 in early endosomes after endocytosis of TLR4. In TLR2 signaling, physically bridges TLR2 and MYD88 and is required for the TLR2-dependent movement of MYD88 to endosomes following ligand engagement. Involved in IL-18 signaling and is proposed to function as a sorting adapter for MYD88 in IL-18 signaling during adaptive immune response. Forms a complex with RAB11FIP2 that is recruited to the phagosomes to promote the activation of the actin-regulatory GTPases RAC1 and CDC42 and subsequent phagocytosis of Gram-negative bacteria.; FUNCTION: [Isoform 2]: Proposed to inhibit LPS-TLR4 signaling at the late endosome by interaction with isoform 1 thereby disrupting the association of isoform 1 with TICAM1. May be involved in TLR4 degradation in late endosomes. KEYWORDS: 3D-structure;Alternative splicing;Cell membrane;Cell projection;Cytoplasm;Endoplasmic reticulum;Endosome;Golgi apparatus;Immunity;Inflammatory response;Innate immunity;Lipoprotein;Membrane;Myristate;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: [Isoform 1]: Cytoplasm Golgi apparatus. Cell membrane Endoplasmic reticulum. Early endosome membrane. Late endosome membrane. Cell projection, phagocytic cup Note=Localized to the plasma membrane as a result of myristoylation. Phosphorylation on Ser-16 leads to its depletion from the membrane. Upon LPS stimulation colcoalizes with isoform 2 in late endosomes.; SUBCELLULAR LOCATION: [Isoform 2]: Endoplasmic reticulum. Early endosome membrane. Late endosome membrane. Note=Translocates to late endosomes upon LPS stimulation where it colcoalizes with isoform 1. " Q86XR8,"PROTEIN NAMES: Centrosomal protein of 57 kDa (Cep57) (FGF2-interacting protein) (Testis-specific protein 57) (Translokin) PROTEIN FAMILY: Translokin family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the translokin family. FUNCTION: Centrosomal protein which may be required for microtubule attachment to centrosomes. May act by forming ring-like structures around microtubules. Mediates nuclear translocation and mitogenic activity of the internalized growth factor FGF2, but that of FGF1. KEYWORDS: 3D-structure;Alternative splicing;Coiled coil;Cytoplasm;Cytoskeleton;Microtubule;Nucleus;Phosphoprotein;Reference proteome SUBCELLULAR LOCATION: Nucleus Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome " Q86XT4,"PROTEIN NAMES: E3 ubiquitin-protein ligase TRIM50 (RING-type E3 ubiquitin transferase TRIM50) (Tripartite motif-containing protein 50) PROTEIN FAMILY: TRIM/RBCC family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the TRIM/RBCC family. FUNCTION: E3 ubiquitin-protein ligase that ubiquitinates Beclin-1/BECN1 in a 'Lys-63'-dependent manner enhancing its binding to ULK1. In turn, promotes starvation-induced autophagy activation. Interacts also with p62/SQSTM1 protein and thereby induces the formation and the autophagy clearance of aggresome-associated polyubiquitinated proteins through HDAC6 interaction. Promotes also NLRP3 inflammasome activation by directly inducing NLRP3 oligomerization independent of its E3 ligase function (By similarity). KEYWORDS: Acetylation;Alternative splicing;Coiled coil;Cytoplasm;Metal-binding;Reference proteome;Transferase;Ubl conjugation;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cytoplasm Note=Localizes mainly into discrete cytoplasmic punctuate structures heterogeneous in size and shape containing polyubiquitinated proteins. " Q86Y33,PROTEIN NAMES: Cell division cycle protein 20 homolog B PROTEIN FAMILY: WD repeat CDC20/Fizzy family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the WD repeat CDC20/Fizzy family. FUNCTION: Protein regulator of centriole-deuterosome disengagement and subsequently participates in the ciliogenesis in multiciliated cells (MCCs). KEYWORDS: Alternative splicing;Cytoplasm;Reference proteome;Repeat;WD repeat SUBCELLULAR LOCATION: Cytoplasm. Note=Tightly associated to mature deuterosomes. Q86Y34,"PROTEIN NAMES: Adhesion G protein-coupled receptor G3 (G-protein coupled receptor 97) (G-protein coupled receptor PGR26) PROTEIN FAMILY: G-protein coupled receptor 2 family, Adhesion G-protein coupled receptor (ADGR) subfamily ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily. FUNCTION: Orphan receptor that regulates migration of lymphatic endothelial cells in vitro via the small GTPases RhoA and CDC42. Regulates B-cell development (By similarity). Seems to signal through G-alpha(q)-proteins. KEYWORDS: 3D-structure;Cell membrane;Direct protein sequencing;Disulfide bond;G-protein coupled receptor;Glycoprotein;Membrane;Receptor;Reference proteome;Signal;Transducer;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein " Q86YF9,"PROTEIN NAMES: Cilium assembly protein DZIP1 (DAZ-interacting protein 1/2) (DAZ-interacting zinc finger protein 1) PROTEIN FAMILY: DZIP C2H2-type zinc-finger protein family ORGANISM: Homo sapiens (Human) SIMILARITY: Belongs to the DZIP C2H2-type zinc-finger protein family. FUNCTION: Molecular adapter that recruits protein complexes required for cilium assembly and function to the cilium basal body. At the exit of mitosis, localizes to the basal body and ciliary base of the forming primary cilium where it recruits and activates RAB8A to direct vesicle-mediated transport of proteins to the cilium (By similarity). Also recruits the BBSome, a complex involved in cilium biogenesis, by bridging it to PCM1 at the centriolar satellites of the cilium. It is also required for the recruitment to the cilium basal body of the intraflagellar transport (IFT) machinery as well as the ciliary appendage proteins CEP164 and NINEIN (By similarity). Functions as a regulator of Hedgehog signaling both through its role in cilium assembly but also probably through its ability to retain GLI3 within the cytoplasm (By similarity). It is involved in spermatogenesis through its role in organization of the basal body and assembly of the sperm flagellum. Also indirectly involved in heart development through its function in ciliogenesis. KEYWORDS: Alternative splicing;Cell projection;Cilium;Cilium biogenesis/degradation;Coiled coil;Cytoplasm;Cytoskeleton;Developmental protein;Differentiation;Disease variant;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Spermatogenesis;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, cilium basal body Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriolar satellite Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole Nucleus Nucleus speckle Cytoplasm Note=Localizes to the centriole in cells lacking cilia and to the cilium basal body in ciliated cells. At the exit of mitosis, when the primary cilium is reassembled in daughter cells, localizes at the mother centriole that acts as the basal body of the assembling primary cilium and also accumulates at the ciliary base that constitutes a diffusion barrier for ciliary proteins (By similarity). " Q86YI8,"PROTEIN NAMES: PHD finger protein 13 (Survival time-associated PHD finger protein in ovarian cancer 1) (SPOC1) ORGANISM: Homo sapiens (Human) FUNCTION: Modulates chromatin structure and DNA damage response by regulating key determinants of chromatin compaction and DNA damage response. Binds H3K4me3-containing chromatin and promotes DNA condensation by recruiting corepressors such as TRIM28 and H3K9 methyltransferase SETDB1. Required for normal chromosome condensation during the early stages of mitosis. Required for normal chromosome separation during mitosis. Increases both chromatin-associated levels and activity of H3K9 methyltransferases, such as SETDB1, thus enhancing H3K9 trimethylation. Essential for testicular stem-cell differentiation and sustained spermatogenesis (By similarity). KEYWORDS: 3D-structure;Cell cycle;Cell division;Chromatin regulator;DNA condensation;Metal-binding;Mitosis;Nucleus;Reference proteome;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus Nucleus, nucleoplasm Note=Predominantly bound to chromatin, but a minor proportion is also detected in the nucleoplasm. " Q86YS3,"PROTEIN NAMES: Rab11 family-interacting protein 4 (FIP4-Rab11) (Rab11-FIP4) (Arfophilin-2) ORGANISM: Homo sapiens (Human) FUNCTION: Acts as a regulator of endocytic traffic by participating in membrane delivery. Required for the abcission step in cytokinesis, possibly by acting as an 'address tag' delivering recycling endosome membranes to the cleavage furrow during late cytokinesis. In case of infection by HCMV (human cytomegalovirus), may participate in egress of the virus out of nucleus; this function is independent of ARF6. KEYWORDS: Alternative splicing;Calcium;Coiled coil;Cytoplasm;Cytoplasmic vesicle;Cytoskeleton;Endosome;Host-virus interaction;Membrane;Metal-binding;Reference proteome;Transport SUBCELLULAR LOCATION: Endosome Cytoplasm, cytoskeleton, spindle Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Recycling endosome membrane ; Peripheral membrane protein. Cleavage furrow Midbody Cytoplasmic vesicle Note=Recruited to the cleavage furrow and the midbody during cytokinesis. " Q88JU3,"PROTEIN NAMES: 3-dehydroshikimate dehydratase (DSD) PROTEIN FAMILY: Bacterial two-domain DSD family ORGANISM: Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) SIMILARITY: Belongs to the bacterial two-domain DSD family. FUNCTION: Catalyzes the conversion of 3-dehydroshikimate to protocatechuate (3,4-dihydroxybenzoate), a common intermediate of quinate and shikimate degradation pathways. PATHWAY: Aromatic compound metabolism; 3,4-dihydroxybenzoate biosynthesis. KEYWORDS: 3D-structure;Cobalt;Lyase;Magnesium;Manganese;Metal-binding;Nickel;Reference proteome " Q89ZI2,PROTEIN NAMES: O-GlcNAcase BT_4395 (Beta-N-acetylglucosaminidase) (Beta-N-acetylhexosaminidase) (Beta-hexosaminidase) (Hexosaminidase B) (N-acetyl-beta-glucosaminidase) PROTEIN FAMILY: Glycosyl hydrolase 84 family ORGANISM: Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50) SIMILARITY: Belongs to the glycosyl hydrolase 84 family. FUNCTION: Can hydrolyze the glycosidic link of O-GlcNAcylated proteins. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates (in vitro). KEYWORDS: 3D-structure;Glycosidase;Hydrolase;Reference proteome;Signal MISCELLANEOUS: Metal-binding observed in X-ray crystal structures is artifactual. Q89ZI8,"PROTEIN NAMES: Purine nucleoside phosphorylase BT_4389 (Adenosine deaminase BT_4389) (S-methyl-5'-thioadenosine phosphorylase BT_4389) PROTEIN FAMILY: Purine nucleoside phosphorylase YfiH/LACC1 family ORGANISM: Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50) SIMILARITY: Belongs to the purine nucleoside phosphorylase YfiH/LACC1 family. FUNCTION: Purine nucleoside enzyme that catalyzes the phosphorolysis of adenosine and inosine nucleosides, yielding D-ribose 1-phosphate and the respective free bases, adenine and hypoxanthine. Also catalyzes the phosphorolysis of S-methyl-5'-thioadenosine into adenine and S-methyl-5-thio-alpha-D-ribose 1-phosphate. Also has adenosine deaminase activity. KEYWORDS: Copper;Hydrolase;Metal-binding;Oxidoreductase;Reference proteome;Transferase;Zinc " Q8AWC3,"PROTEIN NAMES: Activity-regulated cytoskeleton-associated protein (Activity-regulated gene 3.1 protein homolog) (ARC/ARG3.1) (Arg3.1) PROTEIN FAMILY: ARC/ARG3.1 family ORGANISM: Gallus gallus (Chicken) SIMILARITY: Belongs to the ARC/ARG3.1 family. FUNCTION: Master regulator of synaptic plasticity that self-assembles into virion-like capsids that encapsulate RNAs and mediate intercellular RNA transfer in the nervous system. ARC protein is released from neurons in extracellular vesicles that mediate the transfer of ARC mRNA into new target cells, where ARC mRNA can undergo activity-dependent translation. ARC capsids are endocytosed and are able to transfer ARC mRNA into the cytoplasm of neurons. Acts as a key regulator of synaptic plasticity: required for protein synthesis-dependent forms of long-term potentiation (LTP) and depression (LTD) and for the formation of long-term memory. Regulates synaptic plasticity by promoting endocytosis of AMPA receptors (AMPARs) in response to synaptic activity: this endocytic pathway maintains levels of surface AMPARs in response to chronic changes in neuronal activity through synaptic scaling, thereby contributing to neuronal homeostasis. Acts as a postsynaptic mediator of activity-dependent synapse elimination in the developing cerebellum by mediating elimination of surplus climbing fiber synapses. Accumulates at weaker synapses, probably to prevent their undesired enhancement. This suggests that ARC-containing virion-like capsids may be required to eliminate synaptic material. KEYWORDS: Cell membrane;Cell projection;Coiled coil;Cytoplasm;Cytoskeleton;Developmental protein;Endocytosis;Endosome;Lipoprotein;Membrane;Palmitate;Postsynaptic cell membrane;Reference proteome;RNA-binding;Synapse;Transport SUBCELLULAR LOCATION: Extracellular vesicle membrane ; Lipid-anchor Postsynaptic cell membrane ; Lipid-anchor Synapse Postsynaptic density Early endosome membrane Cell projection, dendrite Cytoplasm, cytoskeleton Cytoplasm, cell cortex Cell projection, dendritic spine Note=Forms virion-like extracellular vesicles that are released from neurons. Enriched in postsynaptic density of dendritic spines. Accumulates at weaker synapses may be required to prevent their undesired enhancement. Associated with the cell cortex of neuronal soma and dendrites. " Q8AXC6,"PROTEIN NAMES: Mast/stem cell growth factor receptor Kit (SCFR) (Tyrosine-protein kinase kit) PROTEIN FAMILY: Protein kinase superfamily, Tyr protein kinase family, CSF-1/PDGF receptor subfamily ORGANISM: Takifugu rubripes (Japanese pufferfish) (Fugu rubripes) SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily. FUNCTION: Tyrosine-protein kinase that acts as a cell-surface receptor for the cytokine kitlg/scf and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KEYWORDS: ATP-binding;Cell membrane;Disulfide bond;Glycoprotein;Immunoglobulin domain;Kinase;Magnesium;Membrane;Metal-binding;Nucleotide-binding;Phosphoprotein;Receptor;Reference proteome;Repeat;Signal;Transferase;Transmembrane;Transmembrane helix;Tyrosine-protein kinase;Ubl conjugation SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein " Q8AXF4,PROTEIN NAMES: F-box only protein 43 (Early mitotic inhibitor 2) (Emi1-related protein 1) (F-box protein 26) (Xerp1) ORGANISM: Xenopus laevis (African clawed frog) FUNCTION: Required to prevent anaphase onset in cytostatic factor-arrested oocytes. Inhibits the anaphase-promoting complex/cyclosome (APC/C) ubiquitin ligase and prevents cyclin degradation. Probably recognizes and binds to some phosphorylated proteins and promotes their ubiquitination and degradation. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Meiosis;Metal-binding;Phosphoprotein;Reference proteome;Ubl conjugation;Ubl conjugation pathway;Zinc;Zinc-finger Q8BFU8,"PROTEIN NAMES: Vesicular glutamate transporter 3 (VGluT3) (Solute carrier family 17 member 8) PROTEIN FAMILY: Major facilitator superfamily, Sodium/anion cotransporter family, VGLUT subfamily ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the major facilitator superfamily. Sodium/anion cotransporter family. VGLUT subfamily. FUNCTION: Multifunctional transporter that transports L-glutamate as well as multiple ions such as chloride, sodium and phosphate. At the synaptic vesicle membrane, mainly functions as an uniporter that mediates the uptake of L-glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. The L-glutamate uniporter activity is electrogenic and is driven by the proton electrochemical gradient, mainly by the electrical gradient established by the vacuolar H(+)-ATPase across the synaptic vesicle membrane. In addition, functions as a chloride channel that allows a chloride permeation through the synaptic vesicle membrane that affects the proton electrochemical gradient and promotes synaptic vesicles acidification (By similarity). At the plasma membrane, following exocytosis, functions as a symporter of Na(+) and phosphate from the extracellular space to the cytoplasm allowing synaptic phosphate homeostasis regulation (By similarity). The symporter activity is electrogenic (By similarity). Moreover, operates synergistically with SLC18A3/VACHT under a constant H(+) gradient, thereby allowing striatal vesicular acetylcholine uptake. KEYWORDS: Cell membrane;Chloride;Chloride channel;Cytoplasmic vesicle;Deafness;Glycoprotein;Hearing;Ion channel;Ion transport;Membrane;Neurotransmitter transport;Phosphate transport;Reference proteome;Sodium;Sodium transport;Symport;Synapse;Synaptosome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane Cell membrane ; Multi-pass membrane protein Synapse, synaptosome MISCELLANEOUS: Mice defective in Slc17a8 are profoundly deaf owing to the absence of glutamate release from hair cells at the first synapse in the auditory pathway. They lack auditory-nerve responses to acoustic stimuli, although auditory brainstem responses could be elicited by electrical stimuli." Q8BFW4,"PROTEIN NAMES: E3 ubiquitin-protein ligase TRIM65 (Tripartite motif-containing protein 65) PROTEIN FAMILY: TRIM/RBCC family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the TRIM/RBCC family. FUNCTION: E3 ubiquitin ligase that plays a role in several processes including innate immnity, autophagy or inflammation. Negatively regulates miRNAs by modulating the ubiquitination and stability of TNRC6A, a protein involved in RNA-mediated gene silencing by both micro-RNAs (miRNAs) and short interfering RNAs. This ubiquitination results in the suppressed expression of miR-138-5p leading to increased autophagy (By similarity). Upon enteroviral infection, promotes 'Lys-63'-mediated ubiquitination activation of IFIH1/MDA5 leading to innate signaling cascade. Mechanistically, selectively recognizes MDA5 filaments that occur on dsRNAs (By similarity). Plays also a role in limitation of inflammation through different mechanisms. First, promotes 'Lys-48'-mediated ubiquitination of VCAM1 leading to its degradation and limitation of LPS-induced lung inflammation. In addition, negatively regulates inflammasome activation by promoting 'lys48'-linked ubiquitination of NLRP3 which is critical for the inhibition of NLRP3 inflammasome activation in resting macrophages. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Acetylation;Alternative splicing;Coiled coil;Cytoplasm;Immunity;Innate immunity;Metal-binding;Phosphoprotein;Reference proteome;Transferase;Zinc;Zinc-finger SUBCELLULAR LOCATION: Cytoplasm " Q8BG36,"PROTEIN NAMES: Doublesex- and mab-3-related transcription factor 2 (Doublesex-like 2 protein) (Terra) PROTEIN FAMILY: DMRT family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the DMRT family. FUNCTION: Transcriptional activator that directly regulates early activation of the myogenic determination gene MYF5 by binding in a sequence-specific manner to the early epaxial enhancer element of it. Involved in somitogenesis during embryogenesis and somite development and differentiation into sclerotome and dermomyotome. Required for the initiation and/or maintenance of proper organization of the sclerotome, dermomyotome and myotome. Is not required for sex determination and/or differentiation in embryonic development. Also not involved in symmetric somite formation and hence does not regulate the laterality pathway that controls left-right asymmetric organ positioning. KEYWORDS: DNA-binding;Metal-binding;Nucleus;Reference proteome;Transcription;Transcription regulation;Zinc SUBCELLULAR LOCATION: Nucleus " Q8BG60,"PROTEIN NAMES: Thioredoxin-interacting protein (Vitamin D3 up-regulated protein 1) PROTEIN FAMILY: Arrestin family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the arrestin family. FUNCTION: May act as an oxidative stress mediator by inhibiting thioredoxin activity or by limiting its bioavailability. Interacts with COPS5 and restores COPS5-induced suppression of CDKN1B stability, blocking the COPS5-mediated translocation of CDKN1B from the nucleus to the cytoplasm. Inhibits the proteasomal degradation of DDIT4, and thereby contributes to the inhibition of the mammalian target of rapamycin complex 1 (mTORC1) (By similarity). Functions as a transcriptional repressor, possibly by acting as a bridge molecule between transcription factors and corepressor complexes, and over-expression will induce G0/G1 cell cycle arrest. Required for the maturation of natural killer cells. Acts as a suppressor of tumor cell growth. KEYWORDS: Alternative splicing;Cell cycle;Cytoplasm;Disulfide bond;Isopeptide bond;Phosphoprotein;Reference proteome;Transcription;Transcription regulation;Tumor suppressor;Ubl conjugation SUBCELLULAR LOCATION: Cytoplasm " Q8BG93,"PROTEIN NAMES: Nucleotide triphosphate diphosphatase NUDT15 (MutT homolog 2) (mMTH2) (Nucleoside diphosphate-linked moiety X motif 15) (Nudix motif 15) (Nucleoside diphosphate-linked to another moiety X hydrolase 15) (Nudix hydrolase 15) PROTEIN FAMILY: Nudix hydrolase family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the Nudix hydrolase family. FUNCTION: May catalyze the hydrolysis of nucleoside triphosphates including dGTP, dTTP, dCTP, their oxidized forms like 8-oxo-dGTP and the prodrug thiopurine derivatives 6-thio-dGTP and 6-thio-GTP. Could also catalyze the hydrolysis of some nucleoside diphosphate derivatives (By similarity). Hydrolyzes oxidized nucleosides triphosphates like 8-oxo-dGTP in vitro, but the specificity and efficiency towards these substrates are low. Therefore, the potential in vivo sanitizing role of this enzyme, that would consist in removing oxidatively damaged forms of nucleosides to prevent their incorporation into DNA, is unclear. Through the hydrolysis of thioguanosine triphosphates may participate in the catabolism of thiopurine drugs (By similarity). May also have a role in DNA synthesis and cell cycle progression by stabilizing PCNA (By similarity). Exhibits decapping activity towards dpCoA-capped RNAs in vitro. KEYWORDS: Hydrolase;Magnesium;Manganese;Metal-binding;Reference proteome MISCELLANEOUS: Has the ability to complement a mutation of mutT in E.coli and thereby completely suppress the increased frequency of spontaneous mutations." Q8BGF8,"PROTEIN NAMES: Solute carrier family 35 member D3 (Fringe connection-like protein 1) PROTEIN FAMILY: TPT transporter family, SLC35D subfamily ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the TPT transporter family. SLC35D subfamily. FUNCTION: Probable UDP-glucose transmembrane transporter involved in UDP-glucose transport from the cytosol to the lumen of synaptic vesicles. It is involved in platelet dense granules maturation.; FUNCTION: Alternatively, could function as a molecular adapter enhancing the formation of the PI3KC3-C1/AIC/autophagy initiation complex to promote autophagy in dopaminergic neurons. Could also regulate the plasma membrane localization of the D(1A) dopamine receptor/DRD1 and dopamine signaling. KEYWORDS: Cytoplasmic vesicle;Endoplasmic reticulum;Endosome;Membrane;Reference proteome;Sugar transport;Synapse;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane ; Multi-pass membrane protein Early endosome membrane ; Multi-pass membrane protein Endoplasmic reticulum membrane ; Multi-pass membrane protein Note=Active at early endosome membrane in the biosynthesis of mature platelet-dense granules. " Q8BGT0,PROTEIN NAMES: Osteopetrosis-associated transmembrane protein 1 (Chloride channel 7 beta subunit) (Grey-lethal protein) PROTEIN FAMILY: OSTM1 family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the OSTM1 family. FUNCTION: Required for osteoclast and melanocyte maturation and function. KEYWORDS: Glycoprotein;Lysosome;Membrane;Phosphoprotein;Reference proteome;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Lysosome membrane ; Single-pass type I membrane protein Note=Requires CLCN7 to travel to lysosomes. Q8BGT6,"PROTEIN NAMES: MICAL-like protein 1 (Molecule interacting with Rab13) (MIRab13) ORGANISM: Mus musculus (Mouse) FUNCTION: Probable lipid-binding protein with higher affinity for phosphatidic acid, a lipid enriched in recycling endosome membranes. On endosome membranes, may act as a downstream effector of Rab proteins recruiting cytosolic proteins to regulate membrane tubulation. May be involved in a late step of receptor-mediated endocytosis regulating for instance endocytosed-EGF receptor trafficking. Alternatively, may regulate slow endocytic recycling of endocytosed proteins back to the plasma membrane. May indirectly play a role in neurite outgrowth. KEYWORDS: Alternative splicing;Coiled coil;Endocytosis;Endosome;LIM domain;Membrane;Metal-binding;Phosphoprotein;Protein transport;Reference proteome;Transport;Zinc SUBCELLULAR LOCATION: Recycling endosome membrane ; Peripheral membrane protein Late endosome membrane Note=Localization to late endosomes is actin-dependent. Association to tubular recycling endosomes is regulated by RAB35 and ARF6 (By similarity). " Q8BH31,"PROTEIN NAMES: Major facilitator superfamily domain-containing protein 8 PROTEIN FAMILY: Major facilitator superfamily ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the major facilitator superfamily. FUNCTION: Outward-rectifying chloride channel involved in endolysosomal chloride homeostasis, membrane fusion and function. Conducts chloride currents up to hundreds of picoamperes. Regulates lysosomal calcium content by reducing the lysosomal membrane potential, thereby activating TRPML1 channel and further release of lysosomal calcium ions. Regulates the pH in endolysosomal compartments and may contribute to progressive acidification from endosome to lysosome. Permeable to other halides such as iodide and fluoride ions. KEYWORDS: Chloride;Chloride channel;Endosome;Glycoprotein;Ion channel;Ion transport;Lysosome;Membrane;Reference proteome;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Endosome membrane ; Multi-pass membrane protein Lysosome membrane ; Multi-pass membrane protein Note=Sorting to lysosomes involves dileucine-based motif. " Q8BH53,"PROTEIN NAMES: Cilia- and flagella-associated protein 69 ORGANISM: Mus musculus (Mouse) FUNCTION: Cilium- and flagellum-associated protein. In the olfactory epithelium, regulates the speed of activation and termination of the odor response and thus contributes to the robustness of olfactory transduction pathways. Required for sperm flagellum assembly and stability. KEYWORDS: Alternative splicing;Cell projection;Cilium;Differentiation;Flagellum;Olfaction;Reference proteome;Sensory transduction;Spermatogenesis SUBCELLULAR LOCATION: Cell projection, cilium Cell projection, cilium, flagellum Note=Localizes to the midpiece of the sperm flagellum. " Q8BHK6,"PROTEIN NAMES: SLAM family member 7 (Leukocyte cell-surface antigen) (Novel Ly9) (CD antigen CD319) ORGANISM: Mus musculus (Mouse) FUNCTION: Self-ligand receptor of the signaling lymphocytic activation molecule (SLAM) family. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. Activities are controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2. Mediates natural killer (NK) cell activation through a SH2D1A-independent extracellular signal-regulated ERK-mediated pathway (By similarity). Positively regulates NK cell functions by a mechanism dependent on the adapter SH2D1B. In addition to heterotypic NK cells-target cells interactions also homotypic interactions between NK cells may contribute to activation. However, in the absence of SH2D1B, inhibits NK cell function. Acts also inhibitory in T-cells. May play a role in lymphocyte adhesion (By similarity). In LPS-activated monocytes negatively regulates production of pro-inflammatory cytokines (By similarity). KEYWORDS: Adaptive immunity;Alternative splicing;Disulfide bond;Glycoprotein;Immunity;Immunoglobulin domain;Innate immunity;Membrane;Phosphoprotein;Receptor;Reference proteome;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. MISCELLANEOUS: CRACC:CRACC interaction between NK cells and resident Kupffer cells contribute to Poly I:C/D-GalN-induced fulminant hepatitis." Q8BI36,PROTEIN NAMES: JNK1/MAPK8-associated membrane protein (JKAMP) (JNK1-associated membrane protein) (JAMP) (Medulloblastoma antigen MU-MB-50.4 homolog) ORGANISM: Mus musculus (Mouse) FUNCTION: Regulates the duration of MAPK8 activity in response to various stress stimuli. Facilitates degradation of misfolded endoplasmic reticulum (ER) proteins through the recruitment of components of the proteasome and endoplasmic reticulum-associated degradation (ERAD) system. KEYWORDS: Alternative splicing;Endoplasmic reticulum;Glycoprotein;Membrane;Reference proteome;Transmembrane;Transmembrane helix;Ubl conjugation;Unfolded protein response SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Multi-pass membrane protein Q8BI41,"PROTEIN NAMES: Syncytin-B [Cleaved into: Surface protein (SU); Transmembrane protein (TM)] PROTEIN FAMILY: Gamma type-C retroviral envelope protein family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the gamma type-C retroviral envelope protein family. FUNCTION: This endogenous retroviral envelope protein has retained its original fusogenic properties. Together with Syna, participates in trophoblast fusion and the formation of a syncytium during placenta morphogenesis. Synb is specifically involved in formation of syncytiotrophoblast layer II (SynT-II). Promotes myoblast fusion, and may play a role in regeneration of damaged muscle tissue in males. May have immunosuppressive activity. KEYWORDS: Cell membrane;Developmental protein;Disulfide bond;ERV;Glycoprotein;Membrane;Reference proteome;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: [Surface protein]: Cell membrane ; Peripheral membrane protein Note=The surface protein is not anchored to the membrane, but localizes to the extracellular surface through its binding to TM.; SUBCELLULAR LOCATION: [Transmembrane protein]: Cell membrane ; Single-pass membrane protein MISCELLANEOUS: The mouse genome contains a high percentage of proviral-like elements, also called endogenous retroviruses (ERVs) that are the genomic traces of ancient infections of the germline by exogenous retroviruses. Although most of these elements are defective, some have conserved a functional envelope (env) gene, most probably diverted by the host for its benefit." Q8BIA3,PROTEIN NAMES: Homeobox protein Mohawk PROTEIN FAMILY: TALE/IRO homeobox family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the TALE/IRO homeobox family. FUNCTION: May act as a morphogenetic regulator of cell adhesion. Participates in the early events that lead to differentiation. KEYWORDS: Developmental protein;DNA-binding;Homeobox;Nucleus;Reference proteome SUBCELLULAR LOCATION: Nucleus Q8BIJ7,"PROTEIN NAMES: RUN and FYVE domain-containing protein 1 (Rab4-interacting protein) ORGANISM: Mus musculus (Mouse) FUNCTION: Activating adapter involved in cargo sorting from early/recycling endosomes. Regulates retrieval of proteins from endosomes to the trans-Golgi network through interaction with the dynein-dynactin complex. Dual effector of RAB4B and RAB14, mediates a cooperative interaction allowing endosomal tethering and fusion (By similarity). Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in early endosomal trafficking. In oocytes, self-assembles to form a protein matrix which hold together endolysosomes, autophagosomes and proteasomes and generate non-membrane-bound compartments called endo-lysosomal vesicular assemblies (ELVAs). In immature oocytes, ELVAs sequester ubiquitinated protein aggregates and degrade them upon oocyte maturation. KEYWORDS: Coiled coil;Endocytosis;Endosome;Lipid-binding;Membrane;Metal-binding;Phosphoprotein;Protein transport;Reference proteome;Transport;Zinc;Zinc-finger SUBCELLULAR LOCATION: Early endosome membrane ; Peripheral membrane protein Note=In oocytes, localizes in non-membrane-bound compartments called endo-lysosomal vesicular assemblies (ELVAs). " Q8BJ66,"PROTEIN NAMES: Kazal-type serine protease inhibitor domain-containing protein 1 (Bone and odontoblast-expressed protein 1) (Insulin-like growth factor-binding-related protein 10) (IGFBP-rP10) (IGFBP-related protein 10) (Insulin-like growth factor-binding-related protein 4) ORGANISM: Mus musculus (Mouse) FUNCTION: Involved in the proliferation of osteoblasts during bone formation and bone regeneration. Promotes matrix assembly. KEYWORDS: Developmental protein;Differentiation;Disulfide bond;Extracellular matrix;Glycoprotein;Immunoglobulin domain;Osteogenesis;Reference proteome;Secreted;Signal SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix " Q8BJS4,"PROTEIN NAMES: SUN domain-containing protein 2 (Protein unc-84 homolog B) (Sad1/unc-84 protein-like 2) ORGANISM: Mus musculus (Mouse) FUNCTION: As a component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex, involved in the connection between the nuclear lamina and the cytoskeleton. The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning. Specifically, SYNE2 and SUN2 assemble in arrays of transmembrane actin-associated nuclear (TAN) lines which are bound to F-actin cables and couple the nucleus to retrograde actin flow during actin-dependent nuclear movement. Required for interkinetic nuclear migration (INM) and essential for nucleokinesis and centrosome-nucleus coupling during radial neuronal migration in the cerebral cortex and during glial migration. Required for nuclear migration in retinal photoreceptor progenitors implicating association with cytoplasmic dynein-dynactin and kinesin motor complexes, and probably B-type lamins; SUN1 and SUN2 seem to act redundantly. The SUN1/2:KASH5 LINC complex couples telomeres to microtubules during meiosis; SUN1 and SUN2 seem to act at least partial redundantly. Anchors chromosome movement in the prophase of meiosis and is involved in selective gene expression of coding and non-coding RNAs needed for gametogenesis. Required for telomere attachment to nuclear envelope and gametogenesis. May also function on endocytic vesicles as a receptor for Rab5-GDP and participate in the activation of Rab5. KEYWORDS: 3D-structure;Alternative splicing;Coiled coil;Disulfide bond;Endosome;Glycoprotein;Meiosis;Membrane;Nucleus;Phosphoprotein;Reference proteome;Signal-anchor;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Nucleus inner membrane ; Single-pass type II membrane protein Nucleus envelope Endosome membrane ; Single-pass type II membrane protein Note=Colocalizes with KASH5 at sites of telomere attachment in meiocytes. " Q8BLR9,"PROTEIN NAMES: Hypoxia-inducible factor 1-alpha inhibitor (Hypoxia-inducible factor asparagine hydroxylase) ORGANISM: Mus musculus (Mouse) FUNCTION: Hydroxylates HIF-1 alpha at 'Asn-799' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases. Hydroxylates specific Asn residues within ankyrin repeat domains (ARD) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A and several other ARD-containing proteins. Also hydroxylates Asp and His residues within ARDs of ANK1 and TNKS2, respectively. Negatively regulates NOTCH1 activity, accelerating myogenic differentiation (By similarity). Positively regulates ASB4 activity, promoting vascular differentiation. KEYWORDS: Acetylation;Alternative splicing;Cytoplasm;Dioxygenase;Iron;Metal-binding;Nucleus;Oxidoreductase;Reference proteome;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus Cytoplasm Cytoplasm, perinuclear region " Q8BLY1,"PROTEIN NAMES: SPARC-related modular calcium-binding protein 1 (SPARC-related gene protein) (Secreted modular calcium-binding protein 1) (SMOC-1) ORGANISM: Mus musculus (Mouse) FUNCTION: Probable regulator of osteoblast differentiation. Plays essential roles in both eye and limb development. KEYWORDS: Alternative splicing;Basement membrane;Calcium;Developmental protein;Differentiation;Disulfide bond;Extracellular matrix;Glycoprotein;Metal-binding;Reference proteome;Repeat;Secreted;Signal SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix, basement membrane Note=In or around the basement membrane. " Q8BN82,"PROTEIN NAMES: Sialin (H(+)/nitrate cotransporter) (H(+)/sialic acid cotransporter) (AST) (Solute carrier family 17 member 5) (Vesicular excitatory amino acid transporter) (VEAT) PROTEIN FAMILY: Major facilitator superfamily, Sodium/anion cotransporter family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the major facilitator superfamily. Sodium/anion cotransporter family. FUNCTION: Multifunctional anion transporter that operates via two distinct transport mechanisms, namely proton-coupled anion cotransport and membrane potential-dependent anion transport (By similarity). Electroneutral proton-coupled acidic monosaccharide symporter, with a sugar to proton stoichiometry of 1:1. Exports glucuronic acid and free sialic acid derived from sialoglycoconjugate degradation out of lysosomes, driven by outwardly directed lysosomal pH gradient. May regulate lysosome function and metabolism of sialylated conjugates that impact oligodendrocyte lineage differentiation and myelinogenesis in the central nervous system (By similarity). Electrogenic proton-coupled nitrate symporter that transports nitrate ions across the basolateral membrane of salivary gland acinar cells, with nitrate to proton stoichiometry of 2:1. May contribute to nitrate clearance from serum by salivary glands, where it is further concentrated and secreted in the saliva (By similarity). Uses membrane potential to drive the uptake of acidic amino acids and peptides into synaptic vesicles. Responsible for synaptic vesicular storage of L-aspartate and L-glutamate in pinealocytes as well as vesicular uptake of N-acetyl-L-aspartyl-L-glutamate neuropeptide, relevant to aspartegic-associated glutamatergic neurotransmission and activation of metabotropic receptors that inhibit subsequent transmitter release.; FUNCTION: Receptor for CM101, a polysaccharide produced by group B Streptococcus with antipathoangiogenic properties. KEYWORDS: Alternative splicing;Amino-acid transport;Cell membrane;Cytoplasmic vesicle;Glycoprotein;Lysosome;Membrane;Reference proteome;Symport;Synapse;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Basolateral cell membrane ; Multi-pass membrane protein Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane ; Multi-pass membrane protein Lysosome membrane ; Multi-pass membrane protein " Q8BNX1,"PROTEIN NAMES: C-type lectin domain family 4 member G ORGANISM: Mus musculus (Mouse) FUNCTION: Binds mannose, N-acetylglucosamine (GlcNAc) and fucose, but not galactose, in a Ca(2+)-dependent manner. KEYWORDS: Cell membrane;Coiled coil;Disulfide bond;Glycoprotein;Lectin;Membrane;Reference proteome;Signal-anchor;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type II membrane protein " Q8BR37,"PROTEIN NAMES: E3 ubiquitin-protein ligase NHLRC1 (Malin) (NHL repeat-containing protein 1) (RING-type E3 ubiquitin transferase NHLRC1) ORGANISM: Mus musculus (Mouse) FUNCTION: E3 ubiquitin-protein ligase. Together with the phosphatase EPM2A/laforin, appears to be involved in the clearance of toxic polyglucosan and protein aggregates via multiple pathways. In complex with EPM2A/laforin and HSP70, suppresses the cellular toxicity of misfolded proteins by promoting their degradation through the ubiquitin-proteasome system (UPS). Ubiquitinates the glycogen-targeting protein phosphatase subunits PPP1R3C/PTG and PPP1R3D in a laforin-dependent manner and targets them for proteasome-dependent degradation, thus decreasing glycogen accumulation. Polyubiquitinates EPM2A/laforin and ubiquitinates AGL and targets them for proteasome-dependent degradation. Also promotes proteasome-independent protein degradation through the macroautophagy pathway. PATHWAY: Protein modification; protein ubiquitination. KEYWORDS: Autophagy;Endoplasmic reticulum;Metal-binding;Nucleus;Reference proteome;Repeat;Transferase;Ubl conjugation pathway;Zinc;Zinc-finger SUBCELLULAR LOCATION: Endoplasmic reticulum Nucleus Note=Localizes at the endoplasmic reticulum and, to a lesser extent, in the nucleus. " Q8BU27,PROTEIN NAMES: Protein phosphatase 1M (Protein phosphatase 2C isoform eta) (PP2C-eta) (PP2CE) PROTEIN FAMILY: PP2C family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the PP2C family. KEYWORDS: Alternative splicing;Hydrolase;Magnesium;Manganese;Metal-binding;Nucleus;Protein phosphatase;Reference proteome SUBCELLULAR LOCATION: Nucleus Q8BUI3,"PROTEIN NAMES: Leucine-rich repeat and WD repeat-containing protein 1 (ORC-associated protein) (ORCA) (Origin recognition complex-associated protein) PROTEIN FAMILY: LRWD1 family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the LRWD1 family. FUNCTION: Required for G1/S transition. Recruits and stabilizes the origin recognition complex (ORC) onto chromatin during G1 to establish pre-replication complex (preRC) and to heterochromatic sites in post-replicated cells. Binds a combination of DNA and histone methylation repressive marks on heterochromatin. Binds histone H3 and H4 trimethylation marks H3K9me3, H3K27me3 and H4K20me3 in a cooperative manner with DNA methylation (By similarity). Required for silencing of major satellite repeats. May be important ORC2, ORC3 and ORC4 stability. KEYWORDS: Centromere;Chromatin regulator;Chromosome;Cytoplasm;Cytoskeleton;DNA replication;Kinetochore;Leucine-rich repeat;Nucleus;Phosphoprotein;Reference proteome;Repeat;Telomere;Ubl conjugation;WD repeat SUBCELLULAR LOCATION: Nucleus Chromosome, centromere Chromosome, telomere Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Chromosome, centromere, kinetochore Note=Localizes to heterochromatin during G1 phase. Restricted to centromeres or telomeres as cells progress though S phase. When cells enter mitosis, relocalizes to centromeres. Recruitment to pericentric heterochromatin largely depends on the presence of H3K9me3. " Q8BUR3,PROTEIN NAMES: Forkhead box protein J3 ORGANISM: Mus musculus (Mouse) FUNCTION: Transcriptional activator of MEF2C involved in the regulation of adult muscle fiber type identity and skeletal muscle regeneration. Plays an important role in spermatogenesis. Required for the survival of spermatogonia and participates in spermatocyte meiosis. KEYWORDS: Alternative splicing;Differentiation;DNA-binding;Meiosis;Nucleus;Phosphoprotein;Reference proteome;Spermatogenesis;Transcription;Transcription regulation SUBCELLULAR LOCATION: Nucleus Q8BUV3,"PROTEIN NAMES: Gephyrin [Includes: Molybdopterin adenylyltransferase (MPT adenylyltransferase) (Domain G); Molybdopterin molybdenumtransferase (MPT Mo-transferase) (Domain E)] PROTEIN FAMILY: MoaB/Mog family; MoeA family ORGANISM: Mus musculus (Mouse) SIMILARITY: In the N-terminal section; belongs to the MoaB/Mog family.; SIMILARITY: In the C-terminal section; belongs to the MoeA family. FUNCTION: Microtubule-associated protein involved in membrane protein-cytoskeleton interactions. It is thought to anchor the inhibitory glycine receptor (GLYR) to subsynaptic microtubules (By similarity). Acts as a major instructive molecule at inhibitory synapses, where it also clusters GABA type A receptors.; FUNCTION: Has also a catalytic activity and catalyzes two steps in the biosynthesis of the molybdenum cofactor. In the first step, molybdopterin is adenylated. Subsequently, molybdate is inserted into adenylated molybdopterin and AMP is released. PATHWAY: Cofactor biosynthesis; molybdopterin biosynthesis. KEYWORDS: ATP-binding;Cell membrane;Cell projection;Cytoplasm;Cytoskeleton;Lipoprotein;Magnesium;Membrane;Metal-binding;Molybdenum;Molybdenum cofactor biosynthesis;Multifunctional enzyme;Nucleotide-binding;Palmitate;Phosphoprotein;Postsynaptic cell membrane;Reference proteome;Synapse;Transferase SUBCELLULAR LOCATION: Postsynaptic cell membrane ; Lipid-anchor ; Cytoplasmic side Cell membrane ; Lipid-anchor ; Cytoplasmic side Cytoplasm, cytosol Cytoplasm, cytoskeleton Cell projection, dendrite Postsynaptic density Note=Cytoplasmic face of glycinergic postsynaptic membranes (By similarity). Forms clusters at synapses. " Q8BW41,"PROTEIN NAMES: Protein O-linked-mannose beta-1,4-N-acetylglucosaminyltransferase 2 (POMGnT2) (Extracellular O-linked N-acetylglucosamine transferase-like) (Glycosyltransferase-like domain-containing protein 2) PROTEIN FAMILY: Glycosyltransferase 61 family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the glycosyltransferase 61 family. FUNCTION: O-linked mannose beta-1,4-N-acetylglucosaminyltransferase that transfers UDP-N-acetyl-D-glucosamine to the 4-position of the mannose to generate N-acetyl-D-glucosamine-beta-1,4-O-D-mannosylprotein (By similarity). Involved in the biosynthesis of the phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-3-N-acetylglucosamine-beta-4-(phosphate-6-)mannose), a carbohydrate structure present in alpha-dystroglycan (DAG1), which is required for binding laminin G-like domain-containing extracellular proteins with high affinity. PATHWAY: Protein modification; protein glycosylation. KEYWORDS: Endoplasmic reticulum;Glycoprotein;Glycosyltransferase;Membrane;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Endoplasmic reticulum membrane ; Single-pass type II membrane protein " Q8BWM0,"PROTEIN NAMES: Prostaglandin E synthase 2 (GATE-binding factor 1) (GBF-1) (Microsomal prostaglandin E synthase 2) (mPGES-2) [Cleaved into: Prostaglandin E synthase 2 truncated form] PROTEIN FAMILY: GST superfamily ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the GST superfamily. FUNCTION: Isomerase that catalyzes the conversion of PGH2 into the more stable prostaglandin E2 (PGE2) (in vitro). The biological function and the GSH-dependent property of PTGES2 is still under debate (By similarity). In vivo, PTGES2 could form a complex with GSH and heme and would not participate in PGE2 synthesis but would catalyze the degradation of prostaglandin E2 H2 (PGH2) to 12(S)-hydroxy-5(Z),8(E),10(E)-heptadecatrienoic acid (HHT) and malondialdehyde (MDA) (By similarity). May also have transactivation activity toward IFN-gamma (IFNG), possibly via an interaction with CEBPB; however, the relevance of transcription activation activity remains unclear. PATHWAY: Lipid metabolism; prostaglandin biosynthesis. KEYWORDS: Cytoplasm;Fatty acid biosynthesis;Fatty acid metabolism;Golgi apparatus;Isomerase;Lipid biosynthesis;Lipid metabolism;Membrane;Nucleus;Prostaglandin biosynthesis;Prostaglandin metabolism;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus membrane ; Single-pass membrane protein Nucleus Note=According to previous research, some fraction may be nuclear.; SUBCELLULAR LOCATION: [Prostaglandin E synthase 2 truncated form]: Cytoplasm Note=Synthesized as a Golgi membrane-bound protein, which is further cleaved into the predominant soluble truncated form. " Q8BX43,"PROTEIN NAMES: Tumor necrosis factor receptor superfamily member 19L PROTEIN FAMILY: RELT family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the RELT family. FUNCTION: May play a role in apoptosis. Induces activation of MAPK14/p38 and MAPK8/JNK MAPK cascades, when overexpressed. Involved in dental enamel formation. KEYWORDS: Apoptosis;Cell membrane;Cytoplasm;Disulfide bond;Glycoprotein;Membrane;Phosphoprotein;Receptor;Reference proteome;Signal;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Single-pass type I membrane protein Cytoplasm Cytoplasm, perinuclear region " Q8BX57,"PROTEIN NAMES: PX domain-containing protein kinase-like protein (Modulator of Na,K-ATPase) (MONaKA) PROTEIN FAMILY: Protein kinase superfamily ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the protein kinase superfamily. FUNCTION: Binds to and modulates brain Na,K-ATPase subunits ATP1B1 and ATP1B3 and may thereby participate in the regulation of electrical excitability and synaptic transmission. May not display kinase activity. KEYWORDS: Actin-binding;Alternative splicing;Cell membrane;Cytoplasm;Membrane;Reference proteome SUBCELLULAR LOCATION: Cytoplasm Cell membrane ; Peripheral membrane protein Note=Also associates with the plasma membrane. " Q8BXR9,"PROTEIN NAMES: Oxysterol-binding protein-related protein 6 (ORP-6) (OSBP-related protein 6) PROTEIN FAMILY: OSBP family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the OSBP family. FUNCTION: Regulates cellular transport and efflux of cholesterol (By similarity). Plays a role in phosphatidylinositol-4-phophate (PI4P) turnover at the neuronal membrane. Binds via its PH domain PI4P, phosphatidylinositol-4,5-diphosphate, phosphatidylinositol-3,4,5-triphosphate, and phosphatidic acid. Weakly binds 25-hydroxycholesterol (By similarity). KEYWORDS: Acetylation;Alternative splicing;Cell membrane;Cytoplasm;Endoplasmic reticulum;Endosome;Lipid transport;Lipid-binding;Membrane;Nucleus;Phosphoprotein;Reference proteome;Transport SUBCELLULAR LOCATION: Nucleus envelope Cytoplasm, cytosol Endoplasmic reticulum membrane ; Peripheral membrane protein Cell membrane ; Peripheral membrane protein Endosome membrane ; Peripheral membrane protein Note=Co-localizes with OSBPL3 at contact sites between the plasma membrane and the endoplasmic reticulum. " Q8BYZ1,"PROTEIN NAMES: ABI gene family member 3 (New molecule including SH3) (Nesh) PROTEIN FAMILY: ABI family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the ABI family. FUNCTION: Inhibits ectopic tumor cell metastasis of SRD cells. In vitro, reduces cell motility. KEYWORDS: Alternative splicing;Coiled coil;Cytoplasm;Phosphoprotein;Reference proteome;SH3 domain SUBCELLULAR LOCATION: Cytoplasm " Q8C015,"PROTEIN NAMES: Serine/threonine-protein kinase PAK 5 (p21-activated kinase 5) (PAK-5) (p21-activated kinase 7) (PAK-7) PROTEIN FAMILY: Protein kinase superfamily, STE Ser/Thr protein kinase family, STE20 subfamily ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. FUNCTION: Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, proliferation or cell survival. Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates the proto-oncogene RAF1 and stimulates its kinase activity. Promotes cell survival by phosphorylating the BCL2 antagonist of cell death BAD. Phosphorylates CTNND1, probably to regulate cytoskeletal organization and cell morphology. Keeps microtubules stable through MARK2 inhibition and destabilizes the F-actin network leading to the disappearance of stress fibers and focal adhesions (By similarity). KEYWORDS: Apoptosis;ATP-binding;Cytoplasm;Kinase;Mitochondrion;Nucleotide-binding;Nucleus;Phosphoprotein;Reference proteome;Serine/threonine-protein kinase;Transferase SUBCELLULAR LOCATION: Mitochondrion Cytoplasm Nucleus Note=Shuttles between the nucleus and the mitochondria, and mitochondrial localization is essential for the role in cell survival. " Q8C0T0,"PROTEIN NAMES: XK-related protein 8 (mXkr8) [Cleaved into: XK-related protein 8, processed form] PROTEIN FAMILY: XK family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the XK family. FUNCTION: [XK-related protein 8, processed form]: Phospholipid scramblase that promotes phosphatidylserine exposure on apoptotic cell surface. Phosphatidylserine is a specific marker only present at the surface of apoptotic cells and acts as a specific signal for engulfment. Required for the clearance of apoptotic cells, such as engulfment of apoptotic germ cells by Sertoli cells, clearance of senescent neutrophils or regulation of bipolar cell numbers in the retina. Has no effect on calcium-induced exposure of phosphatidylserine (By similarity). Promotes myoblast differentiation and survival. KEYWORDS: Apoptosis;Cell membrane;Cytoplasm;Membrane;Phosphoprotein;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane ; Multi-pass membrane protein Cytoplasm, perinuclear region " Q8C0X2,"PROTEIN NAMES: Sodium/hydrogen exchanger 9B1 (Na(+)/H(+) exchanger-like domain-containing protein 1) (NHE domain-containing protein 1) (Sodium/hydrogen exchanger-like domain-containing protein 1) (Solute carrier family 9 subfamily B member 1) (Testis specific sodium-hydrogen exchanger) (MtsNHE) PROTEIN FAMILY: Monovalent cation:proton antiporter 1 (CPA1) transporter family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family. FUNCTION: Sperm-specific Na(+)/H(+) exchanger involved in intracellular pH regulation of spermatozoa. Involved in sperm motility and fertility. KEYWORDS: Alternative splicing;Antiport;Cell membrane;Cell projection;Cilium;Fertilization;Flagellum;Ion transport;Membrane;Reference proteome;Sodium;Sodium transport;Transmembrane;Transmembrane helix;Transport SUBCELLULAR LOCATION: Cell projection, cilium, flagellum membrane ; Multi-pass membrane protein " Q8C180,"PROTEIN NAMES: Fibroblast growth factor receptor substrate 2 (FGFR substrate 2) (FGFR-signaling adaptor SNT) (FRS2-alpha) (Suc1-associated neurotrophic factor target 1) (SNT-1) ORGANISM: Mus musculus (Mouse) FUNCTION: Adapter protein that links activated FGR and NGF receptors to downstream signaling pathways. Plays an important role in the activation of MAP kinases and in the phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, in response to ligand-mediated activation of FGFR1. Modulates signaling via SHC1 by competing for a common binding site on NTRK1. KEYWORDS: Direct protein sequencing;Lipoprotein;Membrane;Myristate;Phosphoprotein;Reference proteome;Ubl conjugation SUBCELLULAR LOCATION: Membrane ; Lipid-anchor " Q8C1F4,"PROTEIN NAMES: Chondroitin sulfate N-acetylgalactosaminyltransferase 2 (Chondroitin beta-1,4-N-acetylgalactosaminyltransferase 2) (Beta4GalNAcT-2) (GalNAcT-2) PROTEIN FAMILY: Chondroitin N-acetylgalactosaminyltransferase family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the chondroitin N-acetylgalactosaminyltransferase family. FUNCTION: Transfers 1,4-N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the non-reducing end of glucuronic acid (GlcUA). Required for addition of the first GalNAc to the core tetrasaccharide linker and for elongation of chondroitin chains (By similarity). KEYWORDS: Coiled coil;Glycoprotein;Golgi apparatus;Membrane;Metal-binding;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Golgi apparatus, Golgi stack membrane ; Single-pass type II membrane protein " Q8C208,PROTEIN NAMES: Zinc finger protein Eos (Ikaros family zinc finger protein 4) PROTEIN FAMILY: Ikaros C2H2-type zinc-finger protein family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the Ikaros C2H2-type zinc-finger protein family. FUNCTION: DNA-binding protein that binds to the 5'GGGAATRCC-3' Ikaros-binding sequence. Interacts with SPI1 and MITF to repress transcription of the CTSK and ACP5 promoters via recruitment of corepressors SIN3A and CTBP2. May be involved in the development of central and peripheral nervous systems. Essential for the inhibitory function of regulatory T-cells (Treg). Mediates FOXP3-mediated gene silencing in regulatory T-cells (Treg) via recruitment of corepressor CTBP1. KEYWORDS: Acetylation;Alternative splicing;DNA-binding;Isopeptide bond;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Repeat;Repressor;Transcription;Transcription regulation;Ubl conjugation;Zinc;Zinc-finger SUBCELLULAR LOCATION: Nucleus. MISCELLANEOUS: Eos is a Greek word for rising sun. Q8C5L6,"PROTEIN NAMES: Inositol polyphosphate 5-phosphatase K (Phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase) (Phosphatidylinositol-4,5-bisphosphate 5-phosphatase) (Skeletal muscle and kidney-enriched inositol phosphatase) PROTEIN FAMILY: Inositol 1,4,5-trisphosphate 5-phosphatase type II family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the inositol 1,4,5-trisphosphate 5-phosphatase type II family. FUNCTION: Inositol 5-phosphatase which acts on inositol 1,4,5-trisphosphate, inositol 1,3,4,5-tetrakisphosphate, phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol 3,4,5-trisphosphate. Has 6-fold higher affinity for phosphatidylinositol 4,5-bisphosphate than for inositol 1,4,5-trisphosphate (By similarity). Negatively regulates assembly of the actin cytoskeleton. Controls insulin-dependent glucose uptake among inositol 3,4,5-trisphosphate phosphatases; therefore, is the specific regulator for insulin signaling in skeletal muscle. KEYWORDS: Cytoplasm;Endoplasmic reticulum;Hydrolase;Lipid metabolism;Reference proteome SUBCELLULAR LOCATION: Endoplasmic reticulum Cytoplasm Note=Following stimulation with EGF, translocates to membrane ruffles. " Q8C8H8,"PROTEIN NAMES: Kyphoscoliosis peptidase PROTEIN FAMILY: Transglutaminase-like superfamily ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the transglutaminase-like superfamily. FUNCTION: Probable cytoskeleton-associated protease required for normal muscle growth. Involved in function, maturation and stabilization of the neuromuscular junction. May act by cleaving muscle-specific proteins such as FLNC. KEYWORDS: Alternative splicing;Cytoplasm;Cytoskeleton;Hydrolase;Protease;Reference proteome SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton. Cytoplasm, myofibril, sarcomere, Z line. MISCELLANEOUS: Used as a marker for 'occult' heart disease, as its absence does not cause heart pathology in heart." Q8C9A2,"PROTEIN NAMES: Endonuclease V PROTEIN FAMILY: Endonuclease V family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the endonuclease V family. FUNCTION: Endoribonuclease that specifically cleaves inosine-containing RNAs: cleaves RNA at the second phosphodiester bond 3' to inosine. Active against both single-stranded and double-stranded RNAs. Has strong preference for single-stranded RNAs (ssRNAs) toward double-stranded RNAs (dsRNAs). Cleaves mRNAs and tRNAs containing inosine. Also able to cleave structure-specific dsRNA substrates containing the specific sites 5'-IIUI-3' and 5'-UIUU-3'. Inosine is present in a number of RNAs following editing; the function of inosine-specific endoribonuclease is still unclear: it could either play a regulatory role in edited RNAs, or be involved in antiviral response by removing the hyperedited long viral dsRNA genome that has undergone A-to-I editing. Binds branched DNA structures. KEYWORDS: 3D-structure;Alternative splicing;Cytoplasm;DNA-binding;Endonuclease;Hydrolase;Magnesium;Metal-binding;Nuclease;Nucleus;Reference proteome;RNA-binding SUBCELLULAR LOCATION: Cytoplasm Nucleus, nucleolus Cytoplasm, Stress granule Note=Relocalizes to cytoplasmic stress granules upon cellular stress where it colocalizes with PABPC1. MISCELLANEOUS: Was initially characterized as an endodeoxyribonuclease involved in DNA repair. While it shows some weak endodeoxyribonuclease activity in vitro, such activity probably does not exist in vivo." Q8CAS9,"PROTEIN NAMES: Protein mono-ADP-ribosyltransferase PARP9 (ADP-ribosyltransferase diphtheria toxin-like 9) (ARTD9) (B aggressive lymphoma protein homolog) (Poly [ADP-ribose] polymerase 9) (PARP-9) PROTEIN FAMILY: ARTD/PARP family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the ARTD/PARP family. FUNCTION: ADP-ribosyltransferase which, in association with E3 ligase DTX3L, plays a role in DNA damage repair and in immune responses including interferon-mediated antiviral defenses. Within the complex, enhances DTX3L E3 ligase activity which is further enhanced by PARP9 binding to poly(ADP-ribose) (By similarity). In addition, positively regulates DTXL3 protein levels (By similarity). In association with DTX3L and in presence of E1 and E2 enzymes, mediates NAD(+)-dependent mono-ADP-ribosylation of ubiquitin which prevents ubiquitin conjugation to substrates such as histones (By similarity). During DNA repair, PARP1 recruits PARP9/BAL1-DTX3L complex to DNA damage sites via PARP9 binding to ribosylated PARP1 (By similarity). Subsequent PARP1-dependent PARP9/BAL1-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites (By similarity). In response to DNA damage, PARP9-DTX3L complex is required for efficient non-homologous end joining (NHEJ) but the complex function is restrained by PARP9 activity (By similarity). Dispensable for B-cell receptor (BCR) assembly through V(D)J recombination and class switch recombination (CSR). In macrophages, positively regulates pro-inflammatory cytokines production in response to IFNG stimulation by suppressing PARP14-mediated STAT1 ADP-ribosylation and thus promoting STAT1 phosphorylation. Also suppresses PARP14-mediated STAT6 ADP-ribosylation (By similarity). KEYWORDS: ADP-ribosylation;Alternative splicing;Antiviral defense;Cytoplasm;DNA damage;DNA repair;Glycosyltransferase;Immunity;Innate immunity;NAD;Nucleotidyltransferase;Nucleus;Phosphoprotein;Reference proteome;Repeat;Transferase SUBCELLULAR LOCATION: Cytoplasm, cytosol Nucleus Note=Shuttles between the nucleus and the cytosol. Translocates to the nucleus in response to IFNG or IFNB1 stimulation. Export to the cytosol depends on the interaction with DTX3L. Localizes at sites of DNA damage in a PARP1-dependent manner. " Q8CDF7,"PROTEIN NAMES: piRNA biogenesis protein EXD1 (Exonuclease 3'-5' domain-containing protein 1) (Exonuclease 3'-5' domain-like-containing protein 1) (Inactive exonuclease EXD1) (mExd1) PROTEIN FAMILY: EXD1 family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the EXD1 family. FUNCTION: RNA-binding component of the PET complex, a multiprotein complex required for the processing of piRNAs during spermatogenesis. The piRNA metabolic process mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposable elements, preventing their mobilization, which is essential for the germline integrity. The PET complex is required during the secondary piRNAs metabolic process for the PIWIL2 slicing-triggered loading of PIWIL4 piRNAs. In the PET complex, EXD1 probably acts as an RNA adapter. EXD1 is an inactive exonuclease (By similarity). KEYWORDS: Cytoplasm;Meiosis;Reference proteome;RNA-binding;RNA-mediated gene silencing SUBCELLULAR LOCATION: Cytoplasm Note=Component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon activity during meiosis. " Q8CGF6,"PROTEIN NAMES: WD repeat-containing protein 47 (Neuronal enriched MAP interacting protein) (Nemitin) ORGANISM: Mus musculus (Mouse) KEYWORDS: 3D-structure;Cytoplasm;Cytoskeleton;Developmental protein;Microtubule;Phosphoprotein;Reference proteome;Repeat;WD repeat SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton Note=Localization along microtubules is mediated by MAP1S. " Q8CGK5,"PROTEIN NAMES: Interferon lambda receptor 1 (IFN-lambda R1) (Cytokine receptor class-II member 12) (Cytokine receptor family 2 member 12) (CRF2-12) (Interleukin-28 receptor subunit alpha) (IL-28 receptor subunit alpha) (IL-28R-alpha) (IL-28RA) PROTEIN FAMILY: Type II cytokine receptor family ORGANISM: Mus musculus (Mouse) SIMILARITY: Belongs to the type II cytokine receptor family. FUNCTION: The IFNLR1/IL10RB dimer is a receptor for the cytokine ligands IFNL2 and IFNL3 and mediates their antiviral activity. The ligand/receptor complex stimulate the activation of the JAK/STAT signaling pathway leading to the expression of IFN-stimulated genes (ISG), which contribute to the antiviral state. Determines the cell type specificity of the lambda interferon action. Shows a more restricted pattern of expression in the epithelial tissues thereby limiting responses to lambda interferons primarily to epithelial cells of the respiratory, gastrointestinal, and reproductive tracts. Seems not to be essential for early virus-activated host defense in vaginal infection, but plays an important role in Toll-like receptor (TLR)-induced antiviral defense. Plays a significant role in the antiviral immune defense in the intestinal epithelium. KEYWORDS: 3D-structure;Antiviral defense;Disulfide bond;Glycoprotein;Membrane;Receptor;Reference proteome;Signal;Transmembrane;Transmembrane helix;Ubl conjugation SUBCELLULAR LOCATION: Membrane ; Single-pass type I membrane protein " Q8CHT1,"PROTEIN NAMES: Ephexin-1 (Eph-interacting exchange protein) (Neuronal guanine nucleotide exchange factor) ORGANISM: Mus musculus (Mouse) FUNCTION: Acts as a guanine nucleotide exchange factor (GEF) which differentially activates the GTPases RHOA, RAC1 and CDC42. Plays a role in axon guidance regulating ephrin-induced growth cone collapse and dendritic spine morphogenesis. Upon activation by ephrin through EPHA4, the GEF activity switches toward RHOA resulting in its activation. Activated RHOA promotes cone retraction at the expense of RAC1- and CDC42-stimulated growth cone extension. KEYWORDS: Alternative splicing;Cell projection;Cytoplasm;Developmental protein;Differentiation;Guanine-nucleotide releasing factor;Membrane;Neurogenesis;Phosphoprotein;Reference proteome;SH3 domain SUBCELLULAR LOCATION: Cytoplasm Membrane Cell projection, growth cone Note=Associated with membranes. Localizes to axonal growth cones (By similarity). " Q8CIW5,"PROTEIN NAMES: Twinkle mtDNA helicase (Progressive external ophthalmoplegia 1 protein homolog) (T7 gp4-like protein with intramitochondrial nucleoid localization) (T7-like mitochondrial DNA helicase) (Twinkle protein, mitochondrial) ORGANISM: Mus musculus (Mouse) FUNCTION: Mitochondrial helicase involved in mtDNA replication and repair (By similarity). Might have a role in mtDNA repair (By similarity). Has DNA strand separation activity needed to form a processive replication fork for leading strand synthesis which is catalyzed by the formation of a replisome complex with POLG and mtSDB (By similarity). Preferentially unwinds DNA substrates with pre-existing 5'-and 3'- single-stranded tails but is also active on a 5'- flap substrate (By similarity). Can dissociate the invading strand of immobile or mobile D-loop DNA structures irrespective of the single strand polarity of the third strand (By similarity). In addition to its DNA strand separation activity, also has DNA strand annealing, DNA strand-exchange and DNA branch migration activities (By similarity). KEYWORDS: Alternative splicing;ATP-binding;Coiled coil;DNA replication;Helicase;Hydrolase;Isomerase;Lipid-binding;Membrane;Mitochondrion;Mitochondrion inner membrane;Mitochondrion nucleoid;Nucleotide-binding;Reference proteome;Transit peptide SUBCELLULAR LOCATION: Mitochondrion matrix, mitochondrion nucleoid Mitochondrion inner membrane ; Peripheral membrane protein Note=Colocalizes with mtDNA in mitochondrial nucleoids, a nucleoproteins complex consisting of a number of copies of proteins associated with mtDNA, probably involved in mtDNA maintenance and expression (By similarity). Associates with phospholipid membranes via electrostatic binding (By similarity). Preferentially associates with membranes enriched with cardiolipin, a lipid abundant in the mitochondrial inner membrane (By similarity). ATPase and helicase activity is enhanced by binding to lipid membranes (By similarity). " Q8CJ26,"PROTEIN NAMES: Death domain-containing membrane protein NRADD (Neurotrophin receptor homolog-2) (NRH2) (Neurotrophin receptor-alike death domain protein) ORGANISM: Mus musculus (Mouse) FUNCTION: Modulates NTRK1 signaling. Can activate several intracellular signaling pathways, leading to activation of JUN. Promotes apoptosis. Promotes translocation of SORT1 to the cell membrane, and thereby hinders lysosomal degradation of SOTR1 and promotes its interaction with NGFR. KEYWORDS: 3D-structure;Apoptosis;Cell membrane;Glycoprotein;Membrane;Nucleus;Reference proteome;Transmembrane;Transmembrane helix SUBCELLULAR LOCATION: Cell membrane; Single-pass type III membrane protein. Nucleus. Note=Proteolytic processing gives rise to an intracellular domain that translocates to the nucleus. " Q8DP63,"PROTEIN NAMES: Peptidoglycan-N-acetylglucosamine deacetylase (Peptidoglycan GlcNAc deacetylase) (Peptidoglycan N-deacetylase) (PG N-deacetylase) ORGANISM: Streptococcus pneumoniae (strain ATCC BAA-255 / R6) FUNCTION: Catalyzes the deacetylation of N-acetylglucosamine (GlcNAc) residues in peptidoglycan, a modification that confers host lysozyme resistance and contributes to pneumococcal virulence. KEYWORDS: 3D-structure;Carbohydrate metabolism;Cell membrane;Cobalt;Hydrolase;Membrane;Metal-binding;Reference proteome;Transmembrane;Transmembrane helix;Virulence;Zinc SUBCELLULAR LOCATION: Cell membrane ; Single-pass membrane protein " Q8F3Q1,"PROTEIN NAMES: (R)-citramalate synthase CimA (LiCMS) PROTEIN FAMILY: Alpha-IPM synthase/homocitrate synthase family ORGANISM: Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601) SIMILARITY: Belongs to the alpha-IPM synthase/homocitrate synthase family. FUNCTION: Catalyzes the condensation of pyruvate and acetyl-coenzyme A to form (R)-citramalate. Shows strict substrate specificity for pyruvate. Cannot use alpha-ketoisovalerate, alpha-ketobutyrate, alpha-ketoisocaproate, alpha-ketoglutarate or glyoxylate. PATHWAY: Amino-acid biosynthesis; L-isoleucine biosynthesis; 2-oxobutanoate from pyruvate: step 1/3. KEYWORDS: 3D-structure;Amino-acid biosynthesis;Branched-chain amino acid biosynthesis;Isoleucine biosynthesis;Lyase;Manganese;Metal-binding;Pyruvate;Reference proteome;Transferase "