Title of dataset: 
Dataset for the research paper "Local and express probing of enzymatic activity using functional plasmon active optical fiber"
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DOI of the research paper:

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Names/institutions/contact information:
Elena Miliutina 1,2, Elena Stepanova 1, Alina Gorbunova 1, Yuliia Viktosenko 1, Denis Vasilev 1, Anastasiia Skvortsova 1, Vasilii Burtsev 1, Zdenka Kolska 1, Pavel Postnikov,1 Oleksiy Lyutakov 1

1 Department of Solid State Engineering, University of Chemistry and Technology, Technická 5, Prague 16628, Czech Republic
2 Faculty of Science, J. E. Purkyně University, Pasteurova 3544/1, Ústí nad Labem 400 96, Czech Republic

Correspondence – Oleksiy Lyutakov (lyutakoo@vscht.cz)
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Data manager or custodian: Filip Prusa (prusaf@vscht.cz)

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Data collection:
02/2023-04/2025
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Attributes:

GlyIS         – glycoside iodonium salt
UV-Vis 	      – Ultraviolet-Visible Spectroscopy
SEM-EDX       – Scanning Electron Microscopy with Energy-Dispersive X-ray Spectroscopy
AFM	      – atomic force microscopy
RT	      - room temperature
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File formats:
Databases   .csv
Images      .tiff
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Structure of the dataset:

The structure of this dataset is represented by the following description.
Fig. 1 (tiff) Schema of the preparation of optical fiber based sensor for in-situ estimation of enzyme activity and its operation: 
		A, B - plasmon-induced grafting of glycoside chemical moieties (A, B) and their subsequent enzyme-induced cleavage 
		C, D - both leading to the shift of the wavelength position of plasmon absorption band, which can be red in the light-reflection mode. 

Fig. 2 (tiff) SEM and EDX characterization of Au surface morphology and composition before and after the grafting of Gly chemical moieties

Fig. 3 (tiff) AFM measured surface morphology (from left to right: Au surface, Au-Gly surface, Au-Gly surface after interaction with enzyme)

Fig. 4 (csv) UV-Vis optical spectra and gradual shift of plasmon absorption band measured after the probe immersion in the enzyme solution (RT, pH=5.0; enzyme concentration 0.002)

Fig. 5 (csv) Position of plasmon absorption band as a function of optical probe interaction time with enzyme solution (RT, pH=5.0)


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Licenses or restrictions applicable to the data:
Creative Commons Attribution 4.0 International