| Locus | Definition | Primer | Annealing (PCR) | References |
|---|---|---|---|---|
| a Initial denaturation of 2 min at 94 °C, followed by 35 cycles of denaturation at 95 °C for 30 sec, annealing temperature and time for different genes are mentioned in Table 1. b 90 sec of extension at 72 °C and a final extension of 10 minutes at 72 °C. | ||||
| ITS4 | ||||
| ITS | Internal transcribed spacer | a55 °C, 50 sb | White et al. (1990) | |
| ITS5 | ||||
| LR0R | ||||
| LSU | Large subunit 28S | a55 °C, 50 sb | Vilgalys & Hester (1990) | |
| LR5 | ||||
| 5F | ||||
| rpb2 | DNA-dependent RNA polymerase II largest subunit | a57 °C, 55 sb | Liu et al. (1999) | |
| 7cR | ||||
| 728F | ||||
| tef1-α | Translation elongation factor 1 alpha | a56 °C, 50 sb | Zhang et al. (2021) | |
| 986R | ||||
| Bt2a | ||||
| Bt2b | O’Donnell & Cigelnik | |||
| tub2 | Beta-tubulin 2 | a52 °C, 50 sb | (1997), | |
| T1 | Nosratabadi et al. (2018) | |||
| T22 | ||||