TABLE 1. The amplified loci, primers, and PCR thermal cycle protocols were used in this study.
Genes/loci PCR primers (forward/reverse) PCR conditions Reference
LSU LR0R/LR5 95 °C; 5 min (94 °C; 30 s, 55 °C; 50 s, 72 °C; 90 s) × 35 thermal cycles, 72 °C; 10 min Vilgalys & Hester (1990)
ITS SSU ITS1 / ITS4 NS1 / NS4 94 °C; 2 min (95 °C; 30 s, 55 °C; 50 s, 72 °C; 90 s) × 35 thermal cycles, 72 °C; 10 min White et al. (1990)
tef 1-α EF1-983F / EF1-2218R 95 °C; 5 min (95 °C; 30 s, 58 °C; 50 s, 72 °C; 1 min) Rehner & Buckley (2005) × 35 thermal cycles, 72 °C; 10 min