Dataset for the publication:
\nFigure 1. Preparation of the scaffolds for in vitro and in vivo testing. The principle of the drawing method lies in pulling a single fibre from a droplet of polymer solution (A). For in vitro testing, fibers were fixed on inserts fitting in wells of 24-well plate in three directions (B). The scaffold for in vivo testing consisted of a nanofibrous frame on which drawn fibers were fixed in three dimensions (C).
\nFigure 2. PCL fibers drawn in three dimensions visualised by scanning electron microscopy. Magnification 100\u2715, scale bar 500 μm (A), magnification 500\u2715, scale bar 100 μm (B)
\nFigure 3. In vitro test of drawn fibres biocompatibility. Cell metabolic activity of 3T3 fibroblasts cultured on the PCL microfibers increased during the experiment (A) (p<0.05, p<0.001 is indicated by *). 3T3 fibroblasts adhered on fibres were visualised using SEM on day 21 (B; magnification 500\u2715, scale bar 100 μm). The cell distribution was detected using DAPI staining and visualisation using fluorescent microscopy (C, D, E; magnification 10\u2715, scale bar 100 µm). It is visible that cell number increased on day 7 (D) and 21 (E) compared to day 1 (C).
\nFigure 4. In vivo test composite scaffold. The incision in linea alba was sutured (A) and left without any further treatment in the control group. Alternatively, the scaffold was applied on the incision (B) and fixed to the abdominal wall (C). The abdominal wall with implanted scaffold (D) or without (E) was explanted after 6 weeks and examined from the biomechanical and histological point of view.
\nFigure 5. Average values of the elasticity in tension and maximal stress of the non-treated incision and the incision treated with scaffold 6 weeks after surgery. The maximal stress of the tore sample was on the same level but the elasticity in traction of the incision treated with the scaffold reached a significantly higher value. The level of statistical significance for the assays is designated above the mean values (p<0.05).
\nFigure 6. The α-smooth muscle actin immunohistochemical evaluation of the samples and visualisation of the type I collagen stained with the picrosirius red in the polarised light. (A, E) Incision in the tissue samples uncovered with the scaffold. (B, F) The surrounding area uncovered with the scaffold. (C, G) Incision in the tissue samples covered with the scaffold. (D, H) The surrounding area covered with the scaffold. The incision covered with the scaffold (C) contained comparable amount of myofibroblasts as the samples with no covering (A). The surrounding area without scaffold covering contained a very few myofibroblasts (B). Wounds covered with the scaffold showed a more regular distribution of type I collagen (G, H) compared to uncovered defect (E, F). (A, B, C, D) scale bar 100 µm; (E, F, G, H) scale bar 200 µm.
\nFigure 7. Evaluation of microvessels within the samples using CD31 immunohistochemistry and overall trichrome staining of the samples. (A, E) Incision in the tissue samples uncovered with the scaffold. (B, F) The surrounding area uncovered with the scaffold. (C, G) Incision in the tissue samples covered with the scaffold. (D, H) The surrounding area covered with the scaffold. The density of microvessels in the incision was comparable in the samples with (C) and without scaffold (A). The surrounding area covered with scaffold (D) contained more microvessels than the samples without covering (B). The tissue of the incision was less organized and contained adipose tissue (E, F). Wounds supported with scaffolds showed good integration of the scaffold both with granular connective tissue and dense collagenous connective tissue (G, H). Scale bar 100 µm.
\nFigure 8. Paired comparison of the samples covered and uncovered with scaffold. Left column - differences between the healing incision treated with scaffold or without the treatment. The incision treated with scaffold contained greater fraction of type I collagen (A). (A) Fraction of type I collagen in the healing incision. (B) Fraction of actine-positive myofibroblasts (actin) in the healing incision. (C) Density of microvessels in the healing incision. Right column - differences between the healing surrounding areas covered with scaffold or without covering. The tissue of the surrounding area covered with scaffold had a greater fraction of myofibroblasts (B). (A) Fraction of type I collagen in the healing surrounding area. (B) Fraction of myofibroblasts (actin) in the healing surrounding area. (C) Fraction of microvessels in the healing surrounding area.
\nFigure 9. Paired comparison of the healing incision and the surrounding. Left Column - all the samples were covered with the scaffold. The incisions contained greater fraction of type I collagen (A) and higher density of microvessels (C). (A) The quantitative histological evaluation of the content of type I collagen. (B) The quantitative histological evaluation of the content of myofibroblasts (actin). (C) The quantitative histological evaluation of the microvessel density. Right column - all the samples were uncovered with the scaffold. The incisions contained greater fraction of type I collagen (A), greater fraction of myofibroblasts (B) and higher density of microvessels (C). (A) The quantitative histological evaluation of the content of type I collagen. (B) The quantitative histological evaluation of the fraction of myofibroblasts. (C) The quantitative histological evaluation of the microvessel density.
\n", "publication_date": "2025-03-28", "publisher": "Zenodo", "resource_type": { "id": "dataset", "title": { "de": "Datensatz", "en": "Dataset" } }, "rights": [ { "description": { "en": "The Creative Commons Attribution license allows re-distribution and re-use of a licensed work on the condition that the creator is appropriately credited." }, "icon": "cc-by-icon", "id": "cc-by-4.0", "props": { "scheme": "spdx", "url": "https://creativecommons.org/licenses/by/4.0/legalcode" }, "title": { "en": "Creative Commons Attribution 4.0 International" } } ], "title": "S1 The improvement of chirurgical incision healing using scaffold prepared by the fiber drawing method in a rabbit model" }, "parent": { "access": { "owned_by": { "user": "1267636" }, "settings": { "accept_conditions_text": null, "allow_guest_requests": false, "allow_user_requests": false, "secret_link_expiration": 0 } }, "communities": { "default": "6daddedc-bae8-4663-97cf-eb2b729f6b2d", "entries": [ { "access": { "member_policy": "open", "members_visibility": "public", "record_submission_policy": "open", "review_policy": "closed", "visibility": "public" }, "children": { "allow": false }, "created": "2024-01-26T09:53:45.240075+00:00", "custom_fields": { "subjects": [ { "id": "euroscivoc:167" }, { "id": "euroscivoc:153" }, { "id": "euroscivoc:985" }, { "id": "euroscivoc:743" }, { "id": "euroscivoc:157" }, { "id": "euroscivoc:659" }, { "id": "euroscivoc:435" } ] }, "deletion_status": { "is_deleted": false, "status": "P" }, "id": "6daddedc-bae8-4663-97cf-eb2b729f6b2d", "links": {}, "metadata": { "description": "Project aims to create and develop an excellent research Centre, focused on R&D in regenerative medicine therapeutic methods that have the potential to produce applicable results in the long term. Co-financed by the European Union.", "funding": [ { "award": { "number": "CZ.02.01.01/00/22_008/000456", "title": { "en": "Excellent Research in Regenerative Medicine" } }, "funder": { "id": "037n8p820" } } ], "organizations": [ { "id": "03hjekm25" }, { "id": "05xw0ep96" }, { "id": "0157za327" }, { "id": "02acv3g39" }, { "id": "0143w7709" }, { "id": "04wckhb82" }, { "id": "04qxnmv42" }, { "id": "03613d656" }, { "id": "02jtk7k02" }, { "id": "04vjwcp92" }, { "id": "05ggn0a85" } ], "title": "EXREGMED (EXcellence in REGenerative MEDicine)", "type": { "id": "project" } }, "revision_id": 9, "slug": "cz-02-01-01-00-22-008-0004562", "updated": "2026-03-18T10:11:49.096829+00:00" } ], "ids": [ "6daddedc-bae8-4663-97cf-eb2b729f6b2d" ] }, "id": "15101281", "pids": { "doi": { "client": "datacite", "identifier": "10.5281/zenodo.15101281", "provider": "datacite" } } }, "pids": { "doi": { "client": "datacite", "identifier": "10.5281/zenodo.15101282", "provider": "datacite" }, "oai": { "identifier": "oai:zenodo.org:15101282", "provider": "oai" } }, "revision_id": 4, "stats": { "all_versions": { "data_volume": 504473515.0, "downloads": 13, "unique_downloads": 13, "unique_views": 50, "views": 59 }, "this_version": { "data_volume": 504473515.0, "downloads": 13, "unique_downloads": 13, "unique_views": 50, "views": 59 } }, "status": "published", "swh": {}, "updated": "2026-05-15T04:48:34.692291+00:00", "versions": { "index": 1, "is_latest": true } }