case_images: PMC5846225_01
case_images: Lane 1 - DNA ladder, lane 2 - lytA (319 bp) and lane 7 - ply gene (348 bp) for optochin-resistant bile soluble blood isolate
case_images: IJABMR-8-61-g001.jpg
case_images: PMC5846225_01_IJABMR-8-61-g001.jpg
case_images: F1
case_images: Identification by both automated systems VITEK II and MALDI-TOF was S. pneumoniae, and detection of lytA gene and ply gene, encoding for virulence factors autolysin and pneumolysin, respectively, were found to be positive [Figure 1].
case_images: Gene sequencing for lyt A (forward primer)
case_images: IJABMR-8-61-g002.jpg
case_images: PMC5846225_01_IJABMR-8-61-g002.jpg
case_images: F2
case_images: Further Gene sequencing was done, and the isolate was found to have the synonymous single nucleotide polymorphism type of functional mutation in lytA gene [Figures 2 and 3].
case_images: Gene sequencing for lyt A (reverse primer)
case_images: IJABMR-8-61-g003.jpg
case_images: PMC5846225_01_IJABMR-8-61-g003.jpg
case_images: F3
case_images: Further Gene sequencing was done, and the isolate was found to have the synonymous single nucleotide polymorphism type of functional mutation in lytA gene [Figures 2 and 3].