case_images: PMC7494188_01
case_images: Case timeline. UTI, urinary tract infection; MR, magnetic resonance; TUMT, transurethral microwave therapy, CT, computed tomography; PET/CT, positron emission tomography with CT.
case_images: acmi-2-108-g001.jpg
case_images: PMC7494188_01_acmi-2-108-g001.jpg
case_images: F1
case_images: He had a medical history of prostatic hypertrophy complicated with recurrent urinary tract infections (Fig. 1).
case_images: PMC7494188_02
case_images: F. sanguinis
   on Brucella blood agar.
case_images: acmi-2-108-g002.jpg
case_images: PMC7494188_02_acmi-2-108-g002.jpg
case_images: F2
case_images: However, a clear double zone was observed and the colonies close to the metronidazole disk were slightly smaller, a bit greyer and flatter than the colonies in the periphery (Fig. 2).
case_images: (a) Alignment of the partial 16S rRNA gene sequence from the case isolate of 
  F. sanguinis
   with 
  F. sanguinis
   strain CCUG 47711 chromosome, complete genome (sequence ID: CP027226). There are two mismatches at position 202 (G to R=A or G) and at position 439 (A to R) resulting in a match of 99.5 %. (b) Alignment of the sequence obtained from the mixed sequence from the case isolate using the Pathogenomix Ripseq software and database, of 
  M. indoligenes
   with 
  M. indoligenes
   strain MDA2477 (sequence ID: AF527773). There are several mismatches and gaps, resulting in a match of 95.4 %.
case_images: acmi-2-108-g003.jpg
case_images: PMC7494188_02_acmi-2-108-g003.jpg
case_images: F3
case_images: F. sanguinis  with a score of 99.5 was suggested (442 bp sequence, sequence ID: CP027226, coverage of 100%) using the Basic Local Alignment Search Tool (blast) (Fig. 3). For the colonies in the periphery, a mixed chromatogram was found, suggesting DNA from more than one species. The DNA sequence of the  Lachnospiraceae  species (sequence ID: NBBL01000005) was further investigated using blast.  M. indoligenes  was identified with a score of 95.4 (sequence ID: AF527773) (Fig. 3), which is a very high score given that the data originated from a mixed sequence.
case_images: PMC7494188_03