case_images: PMC5626218_01
case_images: - A) Desquamation and crusts in the trunk; B) 1- purulent bulla, 2,3- not
  umbilicated clear blisters.
case_images: 1678-9946-rimtsp-S1678-9946201759041-gf01.jpg
case_images: PMC5626218_01_1678-9946-rimtsp-S1678-9946201759041-gf01.jpg
case_images: f01
case_images: Since 2013 he has been submitted to oral prednisone (1 mg/kg/day) (Figure 1). In 2016, examination of a Gram-stained smear of aspirated material from the purulent bulla (Figure 1, B1) yielded a positive culture for Staphylococcus aureus; two swabs for PCR were taken from non purulent bullous lesions in the trunk (Figure 1, B2, B3).
case_images: - Ethidium bromide stained agarose gel showing the 244 bp HSV amplification
  product and the 220 bp BAC amplification product (segment of the human
  beta-Actin gene used as PCR positive control) and the size marker 100 bp
  (Invitrogen).
case_images: 1678-9946-rimtsp-S1678-9946201759041-gf02.jpg
case_images: PMC5626218_01_1678-9946-rimtsp-S1678-9946201759041-gf02.jpg
case_images: f02
case_images: In 2016, examination of a Gram-stained smear of aspirated material from the purulent bulla (Figure 1, B1) yielded a positive culture for Staphylococcus aureus; two swabs for PCR were taken from non purulent bullous lesions in the trunk (Figure 1, B2, B3). Human Herpesvirus (HHV), in both PCR assays (Figure 2), but we could not distinguish between HSV1 or 2 (herpes simplex virus 1 or herpes simplex virus 2). HSV PCR products were then purified by GE Healthcare commercial kit and the fragments (sense and anti-sense) were sequenced using the Big DyeTM terminator sequencing protocol (Applied Biosystems Inc., Foster City, CA).