case_images: PMC6613454_01
case_images: CT scan aspects of the pulmonary tissue: at D51, before starting the caspofungin treatment (A) and at D78, after the appropriate treatment was done (B).
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case_images: PMC6613454_01_IDR-12-1929-g0001.jpg
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case_images: Another CT scan performed on D51 revealed multiple condensation areas throughout both lungs (Figure 1A). The last CT scan performed on D78 showed a marked reduction of condensation areas in both lungs (Figure 1B).
case_images: Morphological aspects of the T. crustaceus isolate in scanning electron microscopy (ESEM Quanta 200-FEI, Hillsboro - USA): chains of conidia; (A) subspherical to hemispherical, non-ostiolate ascoma; (B) 8-spored asci; (C) thick-walled, finely echinulate ascospores (D).
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case_images: PMC6613454_01_IDR-12-1929-g0002.jpg
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case_images: The main morphological aspects are shown in the Figure 2A-D. Conidia varied from ellipsoidal to cylindrical, rounded at both ends, smooth-walled, hyaline to pale brown, 7-10x3-6 mum (Figure 2A). Superficial ascomata were appearing in crusts, subspherical to hemispherical, non-ostiolate, yellowish-orange to reddish-orange, mostly 600-800 mum diameter, surrounded by a loose cover of aerial hyphae (Figure 2B). Agar, 8-spored, subspherical, irregularly arranged in the centrum (Figure 2C). Ascospores were pale yellow to pale brown, ovoidal to ellipsoidal, 7-8x4.5-6 mum, thick-walled, finely echinulate (Figure 2D).
case_images: Macroscopic aspects of the T. crustaceus isolate after 5 days incubation at different temperatures on Potatoes Dextrose Agar: 30 C (A); 37 C (B).
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case_images: PMC6613454_01_IDR-12-1929-g0003.jpg
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case_images: There was a clear difference between the colonies developed at 30 C and 37 C respectively, with the first exhibiting numerous ascomata on their surface (Figure 3A and B).
case_images: Phylogenetic tree of Thermoascus crustaceus based on confidently aligned internal transcribed spacer (ITS) sequences available in GenBank (constructed with BLASTN 2.9.0+).
case_images: IDR-12-1929-g0004.jpg
case_images: PMC6613454_01_IDR-12-1929-g0004.jpg
case_images: F0004
case_images: High percentages of similarity with other T. crustaceus strains were achieved for our isolate (Figure 4): 98.55% to T. crustaceus NRRL 1563 (GenBank accession No.