case_images: PMC11169740_01
case_images: The phylogenetic analysis was inferred using the Neighbor-Joining method. The optimal tree is shown. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (1,000 replicates) is shown next to the branches. The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. The evolutionary distances were computed using the Kimura 2-parameter method. These distances are measured in the number of substitutions for each site. Four clinical isolates from our investigation and 14 sequences of reference strains retrieved from GenBank data formed the 18 nucleotide sequences that were analyzed. There were a total of 719 positions in the final dataset. Evolutionary analyses were conducted in MEGA X.
case_images: fcimb-14-1394663-g001.jpg
case_images: PMC11169740_01_fcimb-14-1394663-g001.jpg
case_images: f1
case_images: Through phylogenetic analysis, four isolates were accurately identified as H. opuntiae ( Figure 1 ).