case_images: PMC10934222_01
case_images: (A) (1) R2 showed the T-lymphoblastic leukemia (T-ALL) cells and R7 showed the normal T cells of Patient 1, both of them expressed CD7 prior to donor CD7 CAR-T. Pictures 1-3 showed the bone marrow aspirate evaluated by flow cytometry while pictures 4-5 showed the punctured biopsy pathology of the inguinal lymph node before CAR-T and they demonstrated that the T-ALL cells of Patient 1 expressed CD7. Pictures 6-8 showed CD7+ cells from Patient 1 were undetectable after donor CD7 CAR-T. (B) (1) R2 showed the T-ALL cells of Patient 2 prior to donor CD7 CAR-T. Pictures 1-4 showed bone marrow aspirate evaluated by flow cytometry and demonstrated that the T-ALL of Patient 2 expressed CD7. Pictures 5-7 showed CD7+ cells from Patient 2 were undetectable after donor CD7 CAR-T.
case_images: fimmu-15-1333037-g001.jpg
case_images: PMC10934222_02_fimmu-15-1333037-g001.jpg
case_images: f1
case_images: CD7 expression ( Figure 1B ). Chromosome testing revealed a normal karyotype, with no fusion or mutated genes.
case_images: The trends of donor CD7 CAR-T cells and blood cells counts in peripheral blood of Patient 1 and Patient 2. CAR-T, chimeric antigen receptor T; HSCT, hematopoietic stem cell transplantation; PTLD, post-transplant lymphoproliferative disease; CMV, cytomegalovirus.
case_images: fimmu-15-1333037-g002.jpg
case_images: PMC10934222_01_fimmu-15-1333037-g002.jpg
case_images: f2
case_images: The patient's blood cell count did not recover after CRS; the WBCs increased transiently but decreased to severe granulocytopenia again in less than 10 days ( Figure 2 ).
case_images: Timelines of the two patients' disease course. T-ALL, acute T lymphoblastic leukemia; CR, complete remission; CAR-T, chimeric antigen receptor T; allo-, allogeneic; HSCT, hematopoietic stem cell transplantation; PBSCs, peripheral blood stem cells; EBV, Epstein-Barr virus; PTLD, post-transplant lymphoproliferative disease; BOOP, bronchiolitis obliterans with organizing pneumonia; LFS, leukemia-free survival.
case_images: fimmu-15-1333037-g003.jpg
case_images: PMC10934222_02_fimmu-15-1333037-g003.jpg
case_images: f3
case_images: In addition, CD7 CAR-T cells were detected in the peripheral blood, but CD7+ T lymphocytes were undetectable ( Supplementary Figure 3 ). The CD7+, CD4+ and CD8+ cells recovery was shown in  Supplementary Figure 3 .
case_images: PMC10934222_02
case_images: (A) (1) R2 showed the T-lymphoblastic leukemia (T-ALL) cells and R7 showed the normal T cells of Patient 1, both of them expressed CD7 prior to donor CD7 CAR-T. Pictures 1-3 showed the bone marrow aspirate evaluated by flow cytometry while pictures 4-5 showed the punctured biopsy pathology of the inguinal lymph node before CAR-T and they demonstrated that the T-ALL cells of Patient 1 expressed CD7. Pictures 6-8 showed CD7+ cells from Patient 1 were undetectable after donor CD7 CAR-T. (B) (1) R2 showed the T-ALL cells of Patient 2 prior to donor CD7 CAR-T. Pictures 1-4 showed bone marrow aspirate evaluated by flow cytometry and demonstrated that the T-ALL of Patient 2 expressed CD7. Pictures 5-7 showed CD7+ cells from Patient 2 were undetectable after donor CD7 CAR-T.
case_images: fimmu-15-1333037-g001.jpg
case_images: PMC10934222_02_fimmu-15-1333037-g001.jpg
case_images: f1
case_images: CD7 expression ( Figure 1B ). Chromosome testing revealed a normal karyotype, with no fusion or mutated genes.
case_images: Timelines of the two patients' disease course. T-ALL, acute T lymphoblastic leukemia; CR, complete remission; CAR-T, chimeric antigen receptor T; allo-, allogeneic; HSCT, hematopoietic stem cell transplantation; PBSCs, peripheral blood stem cells; EBV, Epstein-Barr virus; PTLD, post-transplant lymphoproliferative disease; BOOP, bronchiolitis obliterans with organizing pneumonia; LFS, leukemia-free survival.
case_images: fimmu-15-1333037-g003.jpg
case_images: PMC10934222_02_fimmu-15-1333037-g003.jpg
case_images: f3
case_images: In addition, CD7 CAR-T cells were detected in the peripheral blood, but CD7+ T lymphocytes were undetectable ( Supplementary Figure 3 ). The CD7+, CD4+ and CD8+ cells recovery was shown in  Supplementary Figure 3 .