case_images: PMC3483708_01
case_images: Preoperative radiograph of 21 and 22 showing a large periapical radiolucency.
case_images: CRIM.DENTISTRY2012-902858.001.jpg
case_images: PMC3483708_01_CRIM.DENTISTRY2012-902858.001.jpg
case_images: fig1
case_images: Periapical radiograph revealed a large diffused periapical radiolucency in relation to 21 and 22 measuring 1.4 cm in diameter (Figure 1).
case_images: Periapical defect after flap reflection.
case_images: CRIM.DENTISTRY2012-902858.002.jpg
case_images: PMC3483708_01_CRIM.DENTISTRY2012-902858.002.jpg
case_images: fig2
case_images: Following administration of local anaesthesia, submarginal incision was given 3 mm apical the marginal gingiva and mucoperiosteal flap was reflected (Figure 2).
case_images: Blood collection from anticubital vein.
case_images: CRIM.DENTISTRY2012-902858.003.jpg
case_images: PMC3483708_01_CRIM.DENTISTRY2012-902858.003.jpg
case_images: fig3
case_images: Intravenous blood (by venipuncturing of the antecubital vein) was collected (Figure 3) in a 10 mL sterile tube without anticoagulant and immediately centrifuged at 3,000 rpm for 10 minutes.
case_images: PRF clot obtained after centrifugation.
case_images: CRIM.DENTISTRY2012-902858.004.jpg
case_images: PMC3483708_01_CRIM.DENTISTRY2012-902858.004.jpg
case_images: fig4
case_images: PRF was easily separated from red corpuscles base (preserving a small RBC layer) using sterile tweezers (Figure 4) just after removal of PPP (platelet-poor plasma) and then transferred into a sterile dappen dish.
case_images: PRF mixed with beta-tricalcium phosphate placed into the defect.
case_images: CRIM.DENTISTRY2012-902858.005.jpg
case_images: PMC3483708_01_CRIM.DENTISTRY2012-902858.005.jpg
case_images: fig5
case_images: PRF was mixed with beta-tricalcium phosphate and augmented into the intrabony defect upto the surrounding bone level (Figure 5).