case_images: PMC3964430_01
case_images: Part of CSGE Gel Showing the Migration Pattern of Exon 14F Amplified Fragments. Fragments With Abnormal Migration Patterns are Marked by Arrow. N: Normal Control, L: Ladder.
case_images: ircmj-16-6727-g001.jpg
case_images: PMC3964430_01_ircmj-16-6727-g001.jpg
case_images: fig8433
case_images: Among all amplified fragments, only PCR product of exon 14F with 686bp long, displayed unmatched shift in CSGE (Figure 1).
case_images: DNA Sequencing Profile of the FVIII Gene (Exon 14F) from the Patient and Normal Controls. This Figure Indicates Transition Point Mutation (GAA GGA) in the Patient DNA Sequence that Replaces a Glutamic acid With a Glycine Residue. Underlining Indicates the Position of Point Mutation.
case_images: ircmj-16-6727-i001.jpg
case_images: PMC3964430_01_ircmj-16-6727-i001.jpg
case_images: fig8434
case_images: PCR product (Figure 2). PCR reaction mixture for exon 14 F containing 1 x PCR buffer, 1.5 mM MgCl2, 0.2 mM dNTP, 10 pmoles of each primer (Forward: 5'-TCCCTACGGAAACTAGCAATG-3' and Reverse: 5'-TCACAAGAGCAGAGCAAAGG-3'), 0.5U of