case_images: PMC11169830_01
case_images: Ultrasonography images showing a hyperechoic lesion at adjacent to the liver and umbilical vein in the 10-day-old female Hanwoo calf.
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case_images: fig1
case_images: The calf was also mildly anemic, as indicated by a hematocrit of 23.1% (Supplementary Figure S1). Ultrasonography revealed hyperechoic pus in the right 9th and 10th intercostals, adjacent to the liver and umbilical vein (Figure 1), as well as bladder distention.
case_images: Gross lesions of the umbilical cord and bladder ligaments vein in the 10-day-old female Hanwoo calf presenting (A) umbilical enlargement, (B) umbilical vein, and (C) umbilical artery with purulent discharge.
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case_images: fig2
case_images: At necropsy, the umbilical cord and bladder ligament, where purulent substances were observed, were collected and transported to the laboratory (Figure 2). BioEdit software and then compared with reference sequences from the National Center for Biotechnology Information database1 to determine its similarity using Geneious Prime 2022.2 software. 2 Phylogenetic analysis of T. pyogenes was performed using the maximum-likelihood method implemented in MEGA11 using the best substitution model. Bootstrap values were calculated by analyzing 1,000 replicates to evaluate the reliability of clusters, and the sequence obtained in this calf was grouped with the reference strains of T. pyogenes (Supplementary Figure S2).
case_images: Microscopic image of Trueperella pyogenes after Gram-staining. Gram-positive coccobacilli were observed from the single colony cultured in greenish-yellow purulent discharge from the umbilical cord at magnification 1,000x.
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case_images: fig3
case_images: Morphologically, distinct colonies were selected, and Gram-staining was performed, which confirmed the presence of Gram-positive bacteria (Figure 3).
case_images: PCR analysis for the detection of Trueperella pyogenes using the plo gene (773 bp) (A). Lane 1: ladder (1 kb), lane 2: umbilical cord, lane 3: lung, lane 4: mesenteric lymph nodes, lane 5: bladder, lane 6: negative control, lane 7: positive control. PCR analysis of virulent genes (B). Lane 1: ladder (1 kb), lane 2: collagen-binding protein (cbpA, 124 bp), lane 3: type A fimbriae (fimA, 605 bp), lane 4: type C fimbriae (fimC, 843 bp), lane 5: type G fimbriae (fimG, 929 bp), lane 6: neuraminidase H (nanH, 781 bp), lane 7: neuraminidase P (nanP, 150 bp).
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case_images: fig4
case_images: T. pyogenes was detected in all the samples examined (Figure 4A).