case_images: PMC11046207_01
case_images: M. capitatus on Sabouraud CAF GEN agar plate.
case_images: gr1.jpg
case_images: PMC11046207_01_gr1.jpg
case_images: fig0005
case_images: Infusion (BHI) broth, then incubated at 37  C overnight. On the first day, Escherichia coli (E. coli) colonies grew up, while M. capitatus appeared on the third day in Sabouraud Dextrose Agar supplemented with chloramphenicol and gentamicin (Sabouraud CAF GEN) (Fig. 1). In detail, three different sets, each consisting of two flasks collected in three different days, were performed; all were positive for E. coli, and two out of the three were positive for M. capitatus.
case_images: (A) Gram-stained smears from positive blood cultures with typical arthroconidial forms of M. capitatus. Magnification, x 100. (B) LCB staining of M. capitatus.
case_images: gr2.jpg
case_images: PMC11046207_01_gr2.jpg
case_images: fig0010
case_images: In addition, Gram staining from positive drainage cultures showed typical arthroconidial forms with purple color (Fig. 2 A). Then, a Lactophenol cotton blue (LCB) staining was performed identifying the presence of fungi with characteristic filamentous form (Fig. 2B).
case_images: Antimicogram of M. capitatus. Anidulafungin: from 0.015 to 8 mug/mL; amphotericin B: from 0.12 to 8 mug/mL; micafungin: from 0.008 to 8 mug/mL; caspofungin: from 0.008 to 8 mug/mL; 5-flucytosine: from 0.06 to 64 mug/mL; posaconazole: from 0.008 to 8 mug/mL; voriconazole: from 0.008 to 8 mug/mL; itraconazole: from 0.015 to 16 mug/mL; fluconazole: from 0.12 to 256 mug/mL. Candida parapsilosis ATCC 22019 and Candida krusei ATCC 6258 were used as quality control strains.
case_images: gr3.jpg
case_images: PMC11046207_01_gr3.jpg
case_images: fig0015
case_images: As reported in Fig. 3, in vitro antifungal susceptibility of M. capitatus was tested by Sensititre microdiluition (Thermo Fisher Scientific) and in Table 1 the relative minimal inhibitory concentration (MIC) values were indicated.