case_images: PMC11300269_01
case_images: Examinations of the patient. (A) Mild inflammation (arrow) due to FCGS was observed within the lesion; x400 magnification; scale bar, 50 mum. (B) Poorly differentiated feline oral squamous cell carcinoma cells (FOSCC) (*); x400 magnification; scale bar, 50 mum. (C) Intraoral photograph of patient with extensive oral ulceration and severe inflammation lesions in the tongue, and oral mucosa on both sides due to FCGS (arrows).
case_images: fvets-11-1443110-g001.jpg
case_images: PMC11300269_01_fvets-11-1443110-g001.jpg
case_images: fig1
case_images: A 5-year-old neutered female Korean domestic shorthair cat weighing 3 kg was presented to our animal hospital after being diagnosed with FOSCC based on a histological examination at another animal hospital (Figures 1A,B). Upon visual inspection, extensive oral ulceration and inflammation lesions were observed in the tongue, and oral mucosa on both sides (right and left mandible) (Figure 1C).
case_images: Facial inflammation before and after MSC transplantation, showing a decrease in facial inflammation (FeDESI scores). (A) Before the injection, the facial inflammation was severe, and bleeding and crust exfoliation occurred several times a day (arrow) (FeDESI score 30). (B) At 12 h after the injection, inflammation and bleeding of the face drastically decreased, and crust exfoliation did not occur (arrow) (FeDESI score 14). (C) At 1 day after the injection, the improved state was maintained (arrow) (FeDESI score 14). (D) At 6 days after the injection, purulent discharge of the face increased again (arrow) (FeDESI score 16). (E) At 10 days after the injection, the erythema and excoriation of the face worsened (arrow) (FeDESI score 20). (F) At 28 days after the injection, severe inflammation of the face (arrow) (FeDESI score 30).
case_images: fvets-11-1443110-g002.jpg
case_images: PMC11300269_01_fvets-11-1443110-g002.jpg
case_images: fig2
case_images: Although antibiotic (cefazolin, 20 mg/kg, PO, q12h), anti-inflammatory drugs [prednisolone (0.5 mg/kg, SC, q12h), dexamethasone (0.1 mg/kg, SC, q12h)], NSAID (meloxicam, 0.04 mg/kg, SC, q24h), pain management (gabapentin, 10 mg/kg, PO, q12h), and fluid therapy were administered for 7 days, the patient showed no improvement, and inflammation at the SCC site worsened (Figure 2A). Twelve hours after the injection, bleeding and facial purulent discharge decreased, and crust exfoliation did not occur (Figure 2B). On Day 1 after the injection, the slight improvement in activity and reduction in purulent discharge were maintained (Figure 2C). On Day 6, purulent discharge at the facial SCC site began to increase (Figure 2D). On Day 10, the erythema and excoriation of the face worsened (Figure 2E). However, the facial purulent discharge gradually worsened, and the patient experienced anorexia, severe pain, and rapid weight loss to 1.68 kg over 28 days (Figure 2F).
case_images: Feline umbilical cord tissue and characterization of fUC-MSCs. (A) Feline umbilical cord tissue. (B) x 50 magnification; (C) x 100 magnification; Passage 3 cells show a spindle-shaped morphology. (B,C) Scale bars, 100 mum. (D) Immunophenotype of fUC-MSCs determined by flow cytometry. (E) Osteogenic differentiation, evaluated using Alizarin Red S staining. (F) Adipogenic differentiation, evaluated using Oil Red O staining. (G) Chondrogenic differentiation, evaluated using Alcian Blue staining. (E-G) Scale bars, 100 mum.
case_images: fvets-11-1443110-g003.jpg
case_images: PMC11300269_01_fvets-11-1443110-g003.jpg
case_images: fig3
case_images: Feline umbilical cords (n = 5) were collected during a cesarean section from a healthy female domestic shorthair cat aged 1 year (Figure 3A). The cells generally exhibited a spindle-shaped morphology under a microscope (Figures 3B,C). The results were evaluated using the FlowJo software (Tree Star, USA). The cells were positive for the MSC markers CD9 and CD44 and negative for CD14 and CD45, which are hematopoietic cell markers (Figure 3D). When cells were cultured in specific differentiation media, MSCs exhibited the ability to differentiate into adipocytes, osteoblasts, and chondroblasts (Figures 3E-G).