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case_images: (A) The Gram stain of the specimen was a cluster of blue-purple, indicating that the organism was Gram-positive bacilli. (B) Weak acid-fast staining was red, indicating that the bacteria was positive for weak acid-fast staining. (C) Dry, rough, and branched bacterial colonies with a little yellowish pigment appeared on the blood agar plate. (D)Under the microscope, the colony morphology was clear.
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case_images: During the third month of hospitalization, the patient's sputum smear showed a large number of gram-positive bacilli outside the white blood cells on Gram staining (Figure 1A) and weak acid-fast staining was positive (Figure 1B). Medium (Figure 1C and D). MALDI-TOF MS of Merieux VITEK MS identity the colonies as Tsukamurella species (Figure 2A), and the DNA (Ezup column bacterial genomic DNA extraction kit, sangon Biotech, Shanghai) of the bacterial strain was extracted for 16S rRNA sequencing (sangon Biotech, Shanghai), and the base pair matching of the sequencing results showed that the Query Cover 99%, E value 0.0, per.ident 99.80%.
case_images: (A) The MALDI-TOF MS spectra. (B) Genome circle map: The first circle shows the size of the genome. The second circle shows the genes predicted by the Forward strand (+) of the genome and the third circle shows the genes predicted by the Reverse strand (-) of the genome. The fourth circle triangle shows the tRNA (the forward and reverse strands are green and blue, respectively), and square represents rRNA (the forward and reverse strands are red and purple, respectively). The fifth circle represents GC Content, and the sixth circle represents GC skew.
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case_images: Medium (Figure 1C and D). MALDI-TOF MS of Merieux VITEK MS identity the colonies as Tsukamurella species (Figure 2A), and the DNA (Ezup column bacterial genomic DNA extraction kit, sangon Biotech, Shanghai) of the bacterial strain was extracted for 16S rRNA sequencing (sangon Biotech, Shanghai), and the base pair matching of the sequencing results showed that the Query Cover 99%, E value 0.0, per.ident 99.80%. Based on the genomic information, a constructed genome circle map described the relational and multidimensional data (Figure 2B).
case_images: Morphology of Tsukamurella tyrosinosolvens strains under electron microscope: 0.5-1mum in size, nonflagellar, noncapsular irregular bacilli.
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case_images: The pure colonies were subjected to scanning electron microscopy to further explore their morphology characteristics (Figure 3) (Xiangya Medical College of Central South University).