case_images: PMC10733445_01
case_images: Isolation and identification of Comamonas kerstersii strain BC020423. (A) Bacterial colonies on Columbia blood agar after being cultured at 37 C in the presence of 5% CO2 for 24 h. (B) The gram-staining reveal that strain BC020423 is gram negative rods. (C) The spectrogram of the strain acquired by MALDI-TOF MS.
case_images: fmed-10-1283769-g001.jpg
case_images: PMC10733445_01_fmed-10-1283769-g001.jpg
case_images: fig1
case_images: After 24 h of incubation, some round, moist, and white colonies grew on the Columbia blood agar and the single strain was named BC020423 (Figure 1A). Subsequently, the strain BC020423 was subjected to Gram staining and Gram-negative bacillus was observed under microscopy (Figure 1B). MALDI-TOF MS (Bruker Daltonik GmbH, Germany) platform after a series of pretreatment according to the manufacturer's instructions (Figure 1C). MALDI-TOF MS identified strain BC020423 as C. kerstersii with a high confidence score of 2.052.
case_images: Phylogenetic tree based on the 16S rRNA gene sequences showing the relationship of isolated strain BC020423 (black arrow) and members within genus Comamonas. The tree was reconstructed by the neighbor-joining method, and Delftia acidovorans IAM 12409T (AB021417) was used as an outgroup. Bootstrap values (>50%) based on 1,000 replicates are shown at branch nodes. T, type strain.
case_images: fmed-10-1283769-g002.jpg
case_images: PMC10733445_01_fmed-10-1283769-g002.jpg
case_images: fig2
case_images: According to the phylogenetic tree, strain BC020423 was clustered with the type strain C. kerstersii LMG 3475T with a bootstrap value of 100% (Figure 2).