case_images: PMC7699305_01
case_images: Standard PA chest x-ray demonstrating widened superior mediastinum with homogenous opacity seen in the right upper lung zone.
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case_images: Around mid-2016, he was referred to the cardiothoracic unit of our institution as his chest x-ray (CXR) showed a widened mediastinum (Figure 1). Pseudomonas phage B3 NC_006548 (19.4 kb region length) was also observed in PA179 with 22 CDS. Prophage visualization and details can be seen in Supplementary Figures 1-4. These CRISPR-Cas systems confer flexible, sequence-specific immunity against bacteriophages and mobile genetic elements. (Supplementary Table S4, Supplementary Figures 1-4).
case_images: Microbiological summary timeline.
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case_images: The timeline of infections encountered in the patient is illustrated in Figure 2. Pseudomonas phage B3 NC_006548 (19.4 kb region length) was also observed in PA179 with 22 CDS. Prophage visualization and details can be seen in Supplementary Figures 1-4. These CRISPR-Cas systems confer flexible, sequence-specific immunity against bacteriophages and mobile genetic elements. (Supplementary Table S4, Supplementary Figures 1-4).
case_images: Circular Genome Representation of P. aeruginosa strain PA179. The DNAPlotter software from the Artemis Package was used standalone. Custom colors were assigned for the Visualization of tRNA and rRNA as well as for CDS, in the forward and reverse strands. The CDS in the forward strand is colored in Violet, CDS in Reverse is colored in blue, tRNA, and rRNA were colored in pink and orange small vertical lines, and the GC content and GC skew are colored in brown and violet, respectively.
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case_images: Table S1). Thereafter, it was rearranged to form the complete genome using Ragout (Reference-Assisted Genome Ordering Utility) tool, which resulted in a single contig of size 6580692 bp (Figure 3). Pseudomonas phage B3 NC_006548 (19.4 kb region length) was also observed in PA179 with 22 CDS. Prophage visualization and details can be seen in Supplementary Figures 1-4. These CRISPR-Cas systems confer flexible, sequence-specific immunity against bacteriophages and mobile genetic elements. (Supplementary Table S4, Supplementary Figures 1-4).
case_images: Genomic Islands in P. aeruginosa PA179.
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case_images: Pseudomonas phage B3 NC_006548 (19.4 kb region length) was also observed in PA179 with 22 CDS. Prophage visualization and details can be seen in Supplementary Figures 1-4. These CRISPR-Cas systems confer flexible, sequence-specific immunity against bacteriophages and mobile genetic elements. (Supplementary Table S4, Supplementary Figures 1-4). The islands were visualized using Island Viewer software (Figure 4 and Supplementary data Table S5).