case_images: PMC4438147_01
case_images: CT scan showing mediastinal enlargement and bilateral infiltrates.
case_images: CRII2015-137368.001.jpg
case_images: PMC4438147_01_CRII2015-137368.001.jpg
case_images: fig1
case_images: Chest X-ray showed mediastinal enlargement and bilateral infiltrates (Figure 1).
case_images: Radiosensitivity assay. Clonogenic survival assays with primary skin were performed as described in Noordzij et al., Blood 2003. In short, primary skin fibroblasts in exponential growth were trypsinized, and 1000-2,000 cells (10,000-20,000 cells for the highest doses) were seeded into 10 cm plastic dishes (2 dishes per dose) and irradiated at room temperature with 0, 1,2,4 or 6 Gy. After 12-14 days, the cells were rinsed with 0.9% NaCl and stained with 0.25% methylene blue for survival assessment. Two independent survival experiments were performed.
case_images: CRII2015-137368.002.jpg
case_images: PMC4438147_01_CRII2015-137368.002.jpg
case_images: fig2
case_images: Radiosensitivity was mildly increased (Figure 2).