case_images: PMC7523628_01
case_images: (a) Skin lesions present at the perineum and tail of a dog (arrows). (b) Gross diagnosis is severe extensive ulcerative haemorrhagic dermatitis (left recumbency, posterior view of lesions).
case_images: acmi-2-109-g001.jpg
case_images: PMC7523628_01_acmi-2-109-g001.jpg
case_images: F1
case_images: The gross skin lesions showed severe extensive ulcerative haemorrhagic dermatitis (Fig. 1a-b).
case_images: Cytologic finding of the lesion shows contorted branching septate fungal hypha. (Giemsa stain).
case_images: acmi-2-109-g002.jpg
case_images: PMC7523628_01_acmi-2-109-g002.jpg
case_images: F2
case_images: There were eosinophilic contorted septate hyphae, measuring 3-5 mum in diameter (Fig. 2).
case_images: Zoospores of P. insidiosum and zoospore germination shows short hypha (arrow) (bar=20 microm).
case_images: acmi-2-109-g003.jpg
case_images: PMC7523628_01_acmi-2-109-g003.jpg
case_images: F3
case_images: In this step, zoospore production was induced and two-flagellate zoospores were demonstrated (Fig. 3).
case_images: The phylogenetic tree was generated using neighbour-joining (NJ) analysis. Based on 10 000 replicates of bootstrap. (a) COX2 and (b) ITS region (*).
case_images: acmi-2-109-g004.jpg
case_images: PMC7523628_01_acmi-2-109-g004.jpg
case_images: F4
case_images: Each amplicon was analysed and used to construct the phylogenetic tree (Fig. 4a, b).