case_images: PMC11263073_01
case_images: Cellular and humoral immune recovery post-HSCT and intrinsic differentiation capacity of sorted naive B cells. (A) Recovery of T and B cells and serum Ig levels until patient-initiated loss-to-follow-up compared to age-matched normal range (grey bars). Star (*) indicates measurement after starting IGRT therapy. (B, C) Sorted naive B cells were cultured for 7 days with MEGACD40L, IL-21, IL-10 and aIgM to initiate differentiation into IgG/IgA-expressing B cells. On day 7, flow cytometry was performed to determine the absolute and relative IgM+, IgG+ and IgA+ B cells within CD19+IgD-CD27+ cells. (D) Supernatant collected on 7 day was used to assess IgG and IgA-levels by ELISA. (E) The expression of AID was assessed by qPCR on collected RNA from unstimulated and stimulated conditions.
case_images: fimmu-15-1397567-g001.jpg
case_images: PMC11263073_01_fimmu-15-1397567-g001.jpg
case_images: f1
case_images: The patient's cellular recovery was normal initially with normal T- and B-cell counts ( Figure 1A ) and without GvHD nor PCR-detectable viral reactivations.