case_images: PMC9300960_01
case_images: Histopathology of male breast adenoid cystic carcinoma (Hematoxylin and eosin [H&E] stain). (A) Microscopic examination of the excised tissue revealed diffuse tumor invasion of the subcutaneous fibers and adipose tissue. (B) Cribriform patterns and basement membrane materials around tumor nest. (C) Cribriform patterns and the cavities contain mucus secretion. (D) Tubular pattern and the cavities contain mucus secretion. (E) Solid patterns. (F) Microcystic patterns. (G) Neural invasion. (H) Adipose invasion. [A: 40x magnification; B-H: 400x magnification].
case_images: fonc-12-905997-g001.jpg
case_images: PMC9300960_01_fonc-12-905997-g001.jpg
case_images: f1
case_images: Microscopic examination of the excised tissue revealed diffuse tumor invasion of the subcutaneous fibers and adipose tissue ( Figure 1A ), with tumor cells arranged in cribriform ( Figures 1B, C ), tubular ( Figure 1D ), solid ( Figure 1E ), and microcystic ( Figure 1F ) patterns. Neural ( Figure 1G ) and adipose tissue ( Figure 1H ) invasion was also observed.
case_images: Immunohistochemical staining and MYB gene detection. (A) Epithelial cells were CD117-positive. (B) Epithelial cells were CK7-positive. (C) Basal cells were CK5/6-positive. (D) P63 highlighted intact myoepithelial cells around the acini. (E) ER was negative. (F) PR was negative. (G) Basement membrane materials were highlighted by collagen type IV staining. (H) Molecular pathology analysis by fluorescence in situ hybridization (FISH) revealed that the tumor was negative for MYB rearrangements. [(A-G) immunohistochemical staining, 400x magnification; (H) FISH, 1000x magnification]. ER, estrogen receptor; PR, progesterone receptor.
case_images: fonc-12-905997-g002.jpg
case_images: PMC9300960_01_fonc-12-905997-g002.jpg
case_images: f2
case_images: The results of immunohistochemical staining ( Figures 2A-G ) were as follows: (i) glandular epithelial cells: E-cadherin(+), P53(+), CK7(+), CD117(+), ER(-), PR(-), HER2(-), Ki67(+, approximately 30%); (ii) myoepithelial/basal cells: P63(+), CK5/6(+), S-100(+), smooth muscle actin-negative [SMA(-)], calponin(-), glial fibrillary acidic protein-negative [GFAP(-)]; (iii) basement membrane materials: collagen type IV(+). Molecular pathology analysis by fluorescence in situ hybridization (FISH) revealed that the tumor was negative for MYB rearrangements ( Figure 2H ).