case_images: PMC11042475_01
case_images: Coronary angiography.
case_images: PGPM-17-163-g0001.jpg
case_images: PMC11042475_01_PGPM-17-163-g0001.jpg
case_images: f0001
case_images: ST elevation in leads II, III, and AVF. Subsequent coronary angiography revealed coronary atherosclerotic heart disease, manifesting as double-vessel disease affecting the anterior descending artery and the right coronary artery (see Figure 1).
case_images: PCR solubility peak curve results.
case_images: PGPM-17-163-g0002.jpg
case_images: PMC11042475_01_PGPM-17-163-g0002.jpg
case_images: f0002
case_images: TL 988 Real-Time PCR System along with Angiotensin Converting Enzyme (ACE) insertion/deletion (I/D) detection kit (Lot.HY221107, Wuxi Ruiqi Gene Biotechnology Co., Ltd.). The detection methodology involved the use of the PCR dissolution curve method, with the positive control DD type exhibiting a melting temperature (Tm) of 51.5 C, and the type II Tm measured at 58.5 C. The proband in this case exhibited ACE D/D genotype, as depicted by the dissolution peak curve illustrated in Figure 2. 
case_images: Sequencing results of the proband. The arrow points to the position of the SNP.
case_images: PGPM-17-163-g0003.jpg
case_images: PMC11042475_01_PGPM-17-163-g0003.jpg
case_images: f0003
case_images: Sanger sequencing revealed a C>A mutation at the 7th base preceding the I/D mutation point (see Figure 3).