case_images: PMC10953639_01
case_images: Left upper limb of a five-year-old patient with Down syndrome and disseminated cutaneous leishmaniasis (A) Before treatment with liposomal amphotericin B (B) After receiving 11 doses of liposomal amphotericin B (3 mg/kg/dose).
case_images: rpmesp-40-01-11481-g001.jpg
case_images: PMC10953639_01_rpmesp-40-01-11481-g001.jpg
case_images: f1
case_images: On January 2019, the patient was admitted again to the Regional Hospital of Cusco and then referred to the National Institute of Child Health in Lima because of the increasing size of the lesions (Figures 1A and 2A). Patients' condition improved after the 11th dose (Figures 1B and 2B), up until the 15th dose (Figure 2C).
case_images: Lower limbs and gluteal area of a five-year-old patient with Down's syndrome and disseminated cutaneous leishmaniasis (A) Before treatment with liposomal amphotericin B (B) After receiving 11 doses of liposomal amphotericin B (3 mg/kg/dose) (C) After receiving 15 doses of liposomal amphotericin B (3 mg/kg/dose).
case_images: rpmesp-40-01-11481-g002.jpg
case_images: PMC10953639_01_rpmesp-40-01-11481-g002.jpg
case_images: f2
case_images: On January 2019, the patient was admitted again to the Regional Hospital of Cusco and then referred to the National Institute of Child Health in Lima because of the increasing size of the lesions (Figures 1A and 2A). Patients' condition improved after the 11th dose (Figures 1B and 2B), up until the 15th dose (Figure 2C).
case_images: PCR-RFLP electrophoresis profile of the Hsp70 using HaeIII enzyme. MM: Molecular marker. Reference strains: Lb: Leishmania (V.) braziliensis, Lp: Leishmania (V.) peruviana, Lg: Leishmania (V.) guyanensis, La: Leishmania (L.) amazonensis, Li: Leishmania (L.) infantum, Ll: Leishmania (V.) lainsoni. M1 and M2: DNA samples from slides with tissue smears. M3 and M4: DNA samples from lancet.
case_images: rpmesp-40-01-11481-g003.jpg
case_images: PMC10953639_01_rpmesp-40-01-11481-g003.jpg
case_images: f3
case_images: Using a polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLP) technique, the region of the gene encoding the heat shock protein (Hsp70) was amplified and then subjected to an enzymatic digestion process with HaeIII and RsaI, according to the methodology described by Montalvo et al. in 2012 (Figures 3 and 4).
case_images: PCR-RFLP electrophoresis profile of the Hsp70 using RsaI enzyme. MM: Molecular marker. Reference strains: Lb: Leishmania (V.) braziliensis, Lp: Leishmania (V.) peruviana. M1 and M2: DNA samples from slides. M3 and M4: DNA samples from lancet.
case_images: rpmesp-40-01-11481-g004.jpg
case_images: PMC10953639_01_rpmesp-40-01-11481-g004.jpg
case_images: f4
case_images: Using a polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLP) technique, the region of the gene encoding the heat shock protein (Hsp70) was amplified and then subjected to an enzymatic digestion process with HaeIII and RsaI, according to the methodology described by Montalvo et al. in 2012 (Figures 3 and 4).