case_images: PMC7128069_01
case_images: Light microscopic appearance. Periodic acid-Schiff (PAS) staining showed crescents formation (a) (x200). Periodic acid methenamine silver (PAM) staining showed mesangium expansion (b) (x200) and segmental duplication of glomerular basement membrane (c) (x200 arrow). Positive immunofluorescent staining of IgG (d), C3 (e), and C1q (f). IgM and IgA were negative.
case_images: CRIN2020-6343521.001.jpg
case_images: PMC7128069_01_CRIN2020-6343521.001.jpg
case_images: fig1
case_images: Immunofluorescence staining showed positivity for immunoglobulin G (IgG), C3, and C1q (Figure 1).
case_images: Electron micrographs of a glomerular tuft. (a) Electron microscopic showed the presence of electron-dense deposits in the glomerular mesangium and capillary walls. (b) Higher magnification reveled deposits as random arrangement of nonbranching fibrils (x7,000 and x20,000).
case_images: CRIN2020-6343521.002.jpg
case_images: PMC7128069_01_CRIN2020-6343521.002.jpg
case_images: fig2
case_images: The deposition of fibrils was noted in the glomerulus on electron microscopy (Figure 2).
case_images: Immunohistochemistry of DNAJB9. (a) Glomeruli are strongly stained (x40). (b) In contrast to the glomerulus, crescent (arrow) is not stained (x400).
case_images: CRIN2020-6343521.003.jpg
case_images: PMC7128069_01_CRIN2020-6343521.003.jpg
case_images: fig3
case_images: In addition, immunohistochemical staining for DNA-J heat shock protein family member B9 (DNAJB9, Anti-DNAJB9 primary antibody was purchased from Proteintech (rabbit polyclonal antibody; catalog no. 13157-1-AP)) showed strong positivity in the glomerulus (Figure 3).