case_images: PMC11268767_01
case_images: Source location of the visceral leishmaniasis case in this report.
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case_images: A 56-year-old male farmer in Jinghe County, Xinjiang Uygur Autonomous Region, northwestern China (Figure 1), presented with a sudden onset of fever and right upper abdominal pain on November 14, 2022.
case_images: The result of the metagenomic next-generation sequencing (mNGS) of the patient's blood sample indicated a coverage of 0.06% of the Leishmania donovani whole genome.
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case_images: PMC11268767_01_IDR-17-3153-g0003.jpg
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case_images: Metagenomic next-generation sequencing (mNGS) of the patient's blood sample indicated a coverage of 0.06% of the L. donovani whole genome of L. donovani (Figure 2).
case_images: Phylogenetic analysis of "Leishmania donovani" based on putative chaperone protein DNAj gene fragment with MEGA7.0. The tree was constructed with the Maximum Likelihood method (ML; bootstrap replicates: 1000). Branch lengths correlate to the number of substitutions inferred according to the scale shown. Sequences of "read 2" obtained in this study are indicated by solid cycle ( ).
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case_images: PMC11268767_01_IDR-17-3153-g0004.jpg
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case_images: Phylogenetic analysis using the putative chaperone protein DNAj gene fragment (corresponding to read 2 in Table S1) revealed that it was clustered with L. donovani within the same clade, with L. infantum, L. chagasi, and L. major as sister clades (Figure 3).