case_images: PMC10601788_01
case_images: Interphase fluorescence in situ hybridization (iFISH) shows reciprocal translocation involving the BCR and ABL1 gene regions which results in the Philadelphia (Ph) chromosome.
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case_images: F1
case_images: Chromosomal analysis showed abnormal karyotype; 46,XY,t(9;22)(q34;q11.2)[31]/46,XY[4] (Fig. 1, 2).
case_images: a-c Cytogenetic analysis performed by G-banding revealed the presence of the classical t(9;22)(q34;q11), ABL, and BCR genes, which are the hallmark of the t(9;22) translocation in the diagnosis of CML patients.
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case_images: PMC10601788_01_cro-2023-0016-0001-533660_F02.jpg
case_images: F2
case_images: Chromosomal analysis showed abnormal karyotype; 46,XY,t(9;22)(q34;q11.2)[31]/46,XY[4] (Fig. 1, 2).
case_images: CBC trends before and after diagnosis of CML.
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case_images: F3
case_images: Two weeks after the initial laboratories, follow-up CBC showed WBCs dropped from 26.8 to 18, and 2 weeks after that, it dropped to 5.3 and basophilia has resolved; serial follow-up results are demonstrated in Figure 3.
case_images: BCR::ABL1/ABL ratio trends after diagnosis of CML.
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case_images: F4
case_images: ABL1 percentage ratio from 78% (IS) at diagnosis to 3% and 0.06% at 3- and 5-month follow-up, respectively (Fig. 4). At 6-month follow-up, he tested negative for a BCR::ABL1 gene fusion by RTqPCR at a sensitivity of 0.00001.