case_images: PMC10858781_01
case_images: (a) Pleural effusion smear showed many atypical epithelioid cells as second foreign population (high magnification of hematoxylin eosin (Hematoxylin and Eosin 200x)). (b) The embedded pleural fluid section showed that the tumor cells were scattered or clustered (medium magnification of Hematoxylin and Eosin 200x). (c) The embedded pleural fluid section showed significant atypia, nuclear disorientation and nucleolus (high magnification of Hematoxylin and Eosin 400x).
case_images: Cytojournal-21-3-g001.jpg
case_images: PMC10858781_01_Cytojournal-21-3-g001.jpg
case_images: F1
case_images: The pleural fluid showed atypical epithelioid cells [Figure 1].
case_images: (a) Tumor cells were seen in the fibrous stroma as epithelioid, with the low magnification of Hematoxylin and Eosin, 40x. (b) The tumor cells were arranged in two ways. The glandular and the solid area are patchy, with a medium magnification of Hematoxylin and Eosin, 100x. (c) A small amount of red blood cells can be seen in the vascular lumen. The cells had significant atypia and high magnification of Hematoxylin and Eosin, 200x. (d) The cells in the solid area were epithelioid or short spindle-shaped, with nuclear deviation, with high magnification of Hematoxylin and Eosin, 400x.
case_images: Cytojournal-21-3-g002.jpg
case_images: PMC10858781_01_Cytojournal-21-3-g002.jpg
case_images: F2
case_images: Hematoxylin-eosin(HE) stained sections are prepared from the tissue cores [Figure 2] and show a solid, cellular neoplasm.
case_images: (a) Immunohistochemical (IHC) CAMTA1 test showed that the tumor nucleus was positive, amplified by the envision method, 100x. (b) IHC CD31 test showed that the tumor nucleus was positive, amplified by the envision method, 100x. (c) IHC ERG test showed that the tumor nucleus was positive, amplified by the envision method, 100x. (d) Fluorescence in situ hybridization (FISH) detection showed that WWTR1-CAMTA1 fusion signal appeared in more than 50% of cells-the FISH method x1000 (400x).
case_images: Cytojournal-21-3-g003.jpg
case_images: PMC10858781_01_Cytojournal-21-3-g003.jpg
case_images: F3
case_images: TTF1, P40, GATA3, CD10, calretinin, smooth muscle actin, or desmin; tumor cells expressed vascular markers such as CAMTA1, CD31, and ERG [Figure 3a-c], and vimentin was positive; the positive index of Ki-67 was approximately 5%. T (1p36) (CAMTA1) gene translocation (+) was detected by fluorescence in situ hybridization (FISH) (Note: red and green separation signals can be seen in > 50% of tumor cells) [Figure 3d].