case_images: PMC11322051_01
case_images: (A) Histology (H&E) of the tonsil at disease recurrence. (B) RT-qPCR amplification plot of marker TCR Dd2.Dd3 (Marker B) at first diagnosis by backtracking analysis in BM (green curves) and at disease recurrence in the tonsil (yellow curves). Quantitative range 1 x 10-3 (red curves). (C) Timeline of the disease monitoring by RT-qPCR with marker IgH.DH3.JH6 (Marker A), by RT-PCR of p190 BCR::ABL1 transcript (P190), and by RT-qPCR with marker TCR Dd2.Dd3 (Marker B). Abbreviations: Dx, diagnosis; D, day; EOI, end-of-induction; EOC, end-of-consolidation; Pos, positive; Neg, negative; T-LBL, T-cell lymphoblastic lymphoma; BCP-ALL, B-cell precursor acute lymphoblastic leukemia.
case_images: fonc-14-1457832-g001.jpg
case_images: PMC11322051_01_fonc-14-1457832-g001.jpg
case_images: f1
case_images: Adeno-tonsillectomy was thus performed, and histological analysis of the tonsils revealed a diffuse lymphoid infiltrate with small- to medium-size blast cells with mitotically active features, finely dispersed chromatin, and scant cytoplasm ( Figure 1A ). TCR Dd2.Dd3), which was identified also in 2.5% of cells in the contemporary BM. This latter marker was not identified at the initial diagnosis by marker screening (reads cutoff >5%). To verify if a blast population positive for this marker was already present at initial diagnosis, backtracking RT-qPCR analysis was performed and showed a low-level positivity (not quantifiable, QR 10-3) ( Figures 1B, C ).