case_images: PMC11381230_01
case_images: Non-keratinized type SCC (A, B) and Immunohistochemistry (C-E) of Case 1. (A) Showed typical features of non-keratinized squamous cell carcinoma; (B) Basal cell carcinoma-like with central necrosis; (C) P16 showed diffuse strong positive, a little faded; (D) P53, wild-type expression pattern; (E) PR, negative,<1%.
case_images: fonc-14-1415816-g001.jpg
case_images: PMC11381230_01_fonc-14-1415816-g001.jpg
case_images: f1
case_images: Microscopically, the endometrial mass showed typical non-keratinizing squamous cell carcinoma with tumor cells distributed in nests resembling high-grade intraepithelial lesions involving glands ( Figure 1A ) and some areas appeared basal cell carcinoma-like with central necrosis ( Figure 1B ). Immunohistochemically, the tumor cells were diffusely positive for P63/P40/CK5/6 and P16 ( Figure 1C ), wild-type P53 ( Figure 1D ); negative for ER/PR/Pax8 ( Figure 1E ); and Ki67 index of about 90%.
case_images: PMC11381230_02
case_images: Keratinized type SCC (A, B) and Immunohistochemistry (C-E) of Case 2. (A) A prominent mass in the uterine body; (B) Pure keratinized squamous cell carcinoma with large amounts of neutrophils aggregated. (C) P16, negative; (D) P53, Aberrant/mutation-type expression pattern; (E) ER, focally positive, between 1% -10%.
case_images: fonc-14-1415816-g002.jpg
case_images: PMC11381230_02_fonc-14-1415816-g002.jpg
case_images: f2
case_images: Macroscopically, a bulging mass measuring 40x35x25 mm involved primarily the endometrium( Figure 2A ), with tumor invasion into the deeper half of the myometrium and lymphovascular space. Microscopically, the tumor cells showed typical morphological features of keratinized squamous cell carcinoma with abundant eosinophilic cytoplasm and keratin pearls ( Figure 2B ), and the cervix was free of disease. Immunohistochemical analysis demonstrated strong positive staining for P63/P40/CK5/6, negative staining for P16/PR, focal positive staining for ER, nearly 100% strong positive staining for P53, and Ki-67 index of about 50% ( Figures 2C-E ).