case_images: PMC9341348_01
case_images: Gross view of the ulcer on the posterolateral border of the tongue.
case_images: autopsy-12-e2021388-g01.jpg
case_images: PMC9341348_01_autopsy-12-e2021388-g01.jpg
case_images: gf01
case_images: On inspection of the lesion, a single crater-like ulcer was present on the left posterolateral border of the tongue, measuring approximately 1.5 cm x 2.0 cm in size, oval in shape, with a velvety red base (Figure 1).
case_images: Photomicrographs of the biopsy. A - dysplastic surface epithelial cells with large, pleomorphic, and hyperchromatic nuclei infiltrating the connective tissue stroma (H&E,4X); B - connective tissue stroma infiltrated with tumor cells (Arrow) (H&E, 10X); C - connective tissue stroma containing dysplastic clear cells along with epithelial cells (20X); D - clear cells with cellular and nuclear pleomorphism (40X).
case_images: autopsy-12-e2021388-g02.jpg
case_images: PMC9341348_01_autopsy-12-e2021388-g02.jpg
case_images: gf02
case_images: Islands of round to polygonal-shaped cells with clear cytoplasm occupied almost 50% of the stromal tissue (Figure 2).
case_images: Photomicrograph of the biopsy. Positive PAS diastase staining in clear cells (arrow) (40X).
case_images: autopsy-12-e2021388-g03.jpg
case_images: PMC9341348_01_autopsy-12-e2021388-g03.jpg
case_images: gf03
case_images: The tumor cells rich in glycogen showed strong positivity for PAS diastase (Figure 3).
case_images: Photomicrograph of the biopsy -A and B - Immunohistochemical staining for cytokeratin (AE1/AE3) showing strong immunoreactivity in the epithelium and epithelial cells present in the connective tissue; A - (4X) and B - (40X).
case_images: autopsy-12-e2021388-g04.jpg
case_images: PMC9341348_01_autopsy-12-e2021388-g04.jpg
case_images: gf04
case_images: AE1/AE3 was strongly positive in the epithelium and the dysplastic epithelial island, keratin pearls, and clear cells in connective tissue stroma suggesting malignancy of epithelial origin (Figure 4).