case_images: PMC10722158_01
case_images: (A) Bone marrow smear of primitive granulocytes under a microscope. (B) Original granulocyte bone marrow smear after peroxidase staining. (C) FISH results of BM cells.
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case_images: PMC10722158_01_fonc-13-1322403-g001.jpg
case_images: f1
case_images: Wright Giemsa staining showed that erythrocyte and lymphocytic proliferation were inhibited ( Figure 1A ). We further performed peroxidase staining test and found that the results of peroxidase staining of the bone marrow cell smear were positive ( Figure 1B ). FISH showed no evidence of PML::RARA fusion invloving t (15, 17)(q24; q21) but showed a higher fluorescence signal (normally 2 red and 2 green in single cell, now 4 red and 4 green), indicating gene amplification or +15,+15,+17,+17, which may originate from aberrant cells ( Figure 1C ).
case_images: (A) Schematic diagram of the formation mechanism of LYN::LINC01900 transcript. (B) Circos plot indicating novel fusions between LYN and LINC01900. (C) Electrophoresis of RT-PCR products from patient showing the LYN::LINC01900 fusion transcript. (D) Partial nucleotide sequences surrounding the junctions of the LYN::LINC01900 fusion transcript.
case_images: fonc-13-1322403-g002.jpg
case_images: PMC10722158_01_fonc-13-1322403-g002.jpg
case_images: f2
case_images: The LYN::LINC01900 transcript, which is a fusion of LYN (NM_001111097.3) exon 8 with LINC01900 (NC_000018.10) exon 2, was detected at a relative transcript expression level of 15.92% normalized to the reference gene ABL1 ( Figures 2A, B ). A band with a length of 230 bp was detected (100 bp of marker), while the reciprocal fusion LINC01900::LYN was not found, consistent with the RNA-targeted sequencing results ( Figure 2C ). Sanger sequencing of the targeted fusion product confirmed the presence of the LINC01900::LYN fusion transcript ( Figure 2D ).
case_images: Timeline of the treatment.
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case_images: PMC10722158_01_fonc-13-1322403-g003.jpg
case_images: f3
case_images: Unfortunately, no further clinical outcomes were monitored because the patient died of COVID-19 infection on December 2022 ( Figure 3 ).