case_images: PMC10834738_01
case_images: Schematic representation of the seminested PCR technique used for the in vivo diagnosis of G. paralysans- and Aelurostrongylus abstrusus infections.
case_images: fvets-11-1322819-g001.jpg
case_images: PMC10834738_01_fvets-11-1322819-g001.jpg
case_images: fig1
case_images: DNA (U2 universal oligonucleotide and G. paralysans-specific oligonucleotide E1: Gp28Sa3) and Aelurostrongylus abstrusus (U1 universal oligonucleotide and A. abstrusus-specific E1: Aa28Ss2) based on previously reported studies (Figure 1). DNA extraction from the serum sample was performed according to the manufacturer instructions, using an E.Z.N.A. 