case_images: PMC11327068_01
case_images: Photomicrograph of a neutrophil containing morulae observed in an abdominal fluid specimen from a dog with disseminated Cladophialophora bantiana phaeohyphomycosis and ehrlichiosis (1,000x, Wright's Giemsa).
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case_images: fig1
case_images: Morulae (Figure 1).
case_images: Photographs of post-mortem examination of a dog with disseminated Cladophialophora bantiana phaeohyphomycosis. (A) Multiple extrahepatic tortuous vessels are noted along the inferior vena cava within the mesentery, consistent with acquired portosystemic shunting. (B) A 1.5 cm in diameter, focal, slightly raised, soft white nodule with red center is observed within the mid-dorsal right parietal lobe of the cerebrum with a red center that extends deep within the cerebrum (arrow). (C) Gross photograph of the liver and of a cross-section of one of the hepatic nodules (inlet).
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case_images: Autopsy demonstrated a large volume of ascitic fluid with multiple extrahepatic tortuous vessels along the inferior vena cava within the mesentery medial to the kidneys, consistent with acquired portosystemic shunts (Figure 2). The brain had a single similar nodule that was approximately 1.5 cm in diameter, located in the right mid-dorsal parietal lobe and contained central hemorrhage (Figure 2).
case_images: Photomicrographs of aspirate and tissue samples from a dog with disseminated Cladophialophora bantiana phaeohyphomycosis and ehrlichiosis. (A) Note the pyogranulomatous inflammation with fungal hyphae on the cytology of a liver nodule aspirate (500x, Wright's Giemsa). Histopathology confirmed severe, multifocal to coalescing, chronic pyogranulomatous hepatitis (B, 200x). Special histochemical staining of a brain nodule with Periodic Acid-Schiff (C, 100x) and Grocott's Methenamine Silver (D, 100x) is highlighting the hyphae which are approximately 5-9 mum in diameter, septate with rare 45-degree angle and-dichotomous branching (arrow).
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case_images: fig3
case_images: Histopathology confirmed severe, multifocal to coalescing, chronic pyogranulomatous hepatitis, nephritis and adrenalitis with fungal sepsis and necrotizing meningoencephalitis (Figure 3).
case_images: Culture of liver swab (Sabouraud dextrose agar medium) from a dog with disseminated phaeohyphomycosis. Note the downy texture and gray black colonies (A) with elliptical conidia in long, wavy, sparsely branched chains (B, wet mount, 400x). The species was confirmed as Cladophialophora bantiana with PCR and genome sequencing.
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case_images: PMC11327068_01_fvets-11-1451299-g004.jpg
case_images: fig4
case_images: A fungus with dark gray, branched hyphae was recovered in culture (Sabouraud dextrose agar medium under 30 C) (Figure 4).