case_images: PMC3535203_01
case_images: Detection of the minor BCR-ABL fusion transcript at the lymphoblastic crisis phase by real-time PCR. The amplification curves for the minor BCR-ABL fusion transcript (A) and ABL1 gene (B) are shown. The ratio of the minor BCR-ABL fusion transcript was calculated as 1.17 normalized copy number (NCN).
case_images: alm-33-80-g001.jpg
case_images: PMC3535203_01_alm-33-80-g001.jpg
case_images: F1
case_images: Ratios of 166.42 (converted to international scale-normalized copy number, IS-NCN) and 1.17 (NCN) were obtained for the major and minor transcripts, respectively (Fig. 1).
case_images: Analysis of RNA samples collected from the chronic and lymphoblastic crisis phase of CML using the HemaVision screening and split-out kits (Bio-Rad Laboratories). The screening kit produced a single band in the M6 lane (A) whereas the spilt-out kit produced a single 397-base pair band in the M6B lane (B), indicating the presence of the major BCR/ABL fusion transcript (b2a2 type) during the chronic phase. However, during the lymphoblastic crisis phase, the screening kit produced 2 bands in lanes M6 and M8 (C) and the spilt-out kit produced a 397-base pair band in the M6B lane (D) and a 320-base pair band in the M8F lane (E). This indicates the simultaneous presence of both the major (M6B, b2a2 type) and minor (M8F, e1a2 type) BCR/ABL fusion transcripts in this patient.
case_images: alm-33-80-g002.jpg
case_images: PMC3535203_01_alm-33-80-g002.jpg
case_images: F2
case_images: Only the major BCR/ABL fusion transcript was detected in the chronic phase sample (Fig. 2A and B). Notably however, both the major and minor BCR/ABL fusion transcripts were detected in the LBC phase sample (Fig. 2C-E).