case_images: PMC6233691_01
case_images: An ulcerated wound on the sole of right foot, measuring 5x4 cm.
case_images: imcrj-11-319Fig1.jpg
case_images: PMC6233691_01_imcrj-11-319Fig1.jpg
case_images: f1-imcrj-11-319
case_images: He complained of recurrent wound infection on his right sole (Figure 1).
case_images: Morphology of B. pseudomallei colonies on ash down agar after 48 hours incubation at 37 C.. Notes: The wrinkled surface of the colonies was appearing. The colony size ranges from 4 to 10 mm.
case_images: imcrj-11-319Fig2.jpg
case_images: PMC6233691_01_imcrj-11-319Fig2.jpg
case_images: f2-imcrj-11-319
case_images: Ashdown agar, these colonies appeared pale pink, and after prolonged incubation became dark purple, dry, and wrinkled in the center (Figure 2). A very strong sweetish-putrid odor was noted, resembling stagnant water.
case_images: Result of PCR using a 16S rRNA primer.. Notes:
  Escherichia coli ATCC 35218 was used as negative control for this amplification assay. A product of 397 bp amplicon was present, which confirmed B. pseudomallei identification.
case_images: imcrj-11-319Fig3.jpg
case_images: PMC6233691_01_imcrj-11-319Fig3.jpg
case_images: f3-imcrj-11-319
case_images: Visualization of a 397 bp-sized DNA bands in electrophoresis with 1% agarose gel confirmed the presence of 16S rRNA gene of B. pseudomallei (Figure 3).