case_images: PMC11102793_01
case_images: Macroscopic findings of congenital tuberculosis in an 8-month-old male British Shorthair cat. White round foci (arrows) with diameters ranging from 3.00 to 5.00 mm can be seen in the A) lung and B) spleen
case_images: vrf-15-203-g001.jpg
case_images: PMC11102793_01_vrf-15-203-g001.jpg
case_images: F1
case_images: During systemic necropsy, white round foci with diameters ranging from 3.00 to 5.00 mm were observed in the lung (Fig. 1A) and spleen (Fig. 1B).
case_images: Histopathological and immunohistochemical analyses of lung and spleen in an 8-month-old male British Shorthair cat with congenital tuberculosis. A) Areas of necrosis without fibrous capsule (asterisks) in the lung (H & E staining bar = 100 microm);  B) Karyorrhectic cells (arrows) and lymphohistiocytic cell infiltration in necrosis areas of the lung; (H & E staining, bar = 50.00 microm); C) Area of necrosis without fibrous capsule (asterisk) in the spleen (H & E staining, bar = 100 microm); D) Acid-fast bacteria (arrowheads) in necrosis areas of the lung (Ziehl-Neelsen staining, bar = 20.00 microm); E) Acid-fast bacteria (arrowheads) in necrosis areas of the spleen (Ziehl-Neelsen staining, bar = 20.00 microm); F) Positive immunostaining (arrows) in the lung using Mycobacterium
  spp. antibody (Immunohistochemical staining, bar = 20.00 microm).
case_images: vrf-15-203-g002.jpg
case_images: PMC11102793_01_vrf-15-203-g002.jpg
case_images: F2
case_images: Microscopically, multi-focal nodular areas consisting of necrosis without fibrous capsule and karyorrhectic cells were also identified in the lung (Figs 2A, and 2B) and spleen (Fig. 2C). The ZN staining of lung (Fig. 2D) and spleen (Fig. 2E) sections revealed acid-fast bacteria presence in the areas of exudative type caseous necrosis. Immunohistochemical staining of lung (Fig. 2F) and spleen sections revealed positive immunostaining using Mycobacterium spp. antibody, particularly in areas of necrosis.