case_images: PMC10656200_01
case_images: On the 4th day of admission, the patient presented with chills, fever, and yellow pus visible as shown on the gauze and the outside of the catheter.
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case_images: fig1
case_images: However, on August 10, 2021, the patient experienced chills and a fever, and yellow pus was visible on the outside of the catheter (Figure 1).
case_images: (a) The patient's blood culture was reported as positive for both aerobic and anaerobic vials. After 24 hours of incubation, the blood plate showed round, moist colonies of beige color. (b) The smear was stained for Gram-positive cocci.
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case_images: fig2
case_images: The blood plate showed off-white, spherical, moist colonies after 24 hours of incubation (Figure 2(a)). The Gram stain of the smear revealed cocci (Figure 2(b)).
case_images: The matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (bioMerieux, France) identified the pathogen as E. gallinarum in the mass spectrometry peak map with 99% identification confidence.
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case_images: fig3
case_images: Figures 3 and 4). For further verification, 16S ribosomal DNA gene sequence analysis was performed on the pure colonies.
case_images: bioMerieux VITEK 2 biochemical ID results.
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case_images: fig4
case_images: Figures 3 and 4). For further verification, 16S ribosomal DNA gene sequence analysis was performed on the pure colonies.
case_images: The 16S ribosomal DNA was amplified by using primers 27F and 1492R. The length of the amplification product was 1500 bp, and the Sanger method was used for sequencing. The gel sequence of the 16S ribosomal DNA gene sequence 100% matched E. gallinarum.
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case_images: fig5
case_images: Amplicons were purified (Figure 5) and sequenced.
case_images: bioMerieux fully automatic microbial identification and drug susceptibility analyzer VITEK 2.
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case_images: fig6
case_images: The results of the drug sensitivity test showed that the bacteria were sensitive to ampicillin, penicillin, linezolid, tigecycline, levofloxacin, teicoplanin, moxifloxacin, and erythromycin and resistant to vancomycin and clindamycin (Figure 6).