case_images: PMC8674942_01
case_images: (A) Abdominal X-ray showing intramural bowel gas also known as pneumatosis intestinalis, (B) culture growth of cream-colored colonies on COS after 24 h of incubation at 37 C in the anaerobic chamber, (C)
  Clostridium neonatale rod-shaped cells using Hitachi SU5000 scanning electron microscope. Scale bar and acquisition settings are shown on the original micrograph, and (D) sporulating C. neonatale cell.
case_images: fped-09-771467-g0001.jpg
case_images: PMC8674942_01_fped-09-771467-g0001.jpg
case_images: F1
case_images: Abdominal X-ray showed pneumatosis intestinalis (Figure 1A). Bruz, France). Colonies were cream-colored and opaque after 24 h of growth (Figure 1B). SU5000 scanning electron microscope (Hitachi High-Tech Corporation, Tokyo, Japan), colonies were bacilli (Figure 1C). Bacterial cells were Gram-positive (not shown). Sporulation was observed after 24 h under aerobic conditions (Figure 1D).
case_images: Whole-genome (nucleotides) based phylogenetic tree highlighting the position of C. neonatale strain Marseille-Q4564 relative to other closely related bacterial taxa.
case_images: fped-09-771467-g0002.jpg
case_images: PMC8674942_01_fped-09-771467-g0002.jpg
case_images: F2
case_images: Taken together, these results confirm the status of this strain belonging to the C. neonatale species (Figure 2).