case_images: PMC6005278_01
case_images: Nodules (one ulcerated) on the distal extremity and digits.
case_images: CRIVEM2016-2878751.001.jpg
case_images: PMC6005278_01_CRIVEM2016-2878751.001.jpg
case_images: fig1
case_images: On dermatological examination, 9 ulcerated and nonulcerated skin nodules were observed, ranged from 1 to 7 cm in diameter, and distributed mainly over bony prominences of distal extremities and digits of the front legs (Figure 1), left elbow, and right hock.
case_images: Hyperkeratosis, depigmentation, and erosions of footpads.
case_images: CRIVEM2016-2878751.002.jpg
case_images: PMC6005278_01_CRIVEM2016-2878751.002.jpg
case_images: fig2
case_images: Other skin lesions included footpad hyperkeratosis, crusting, depigmentation, and erosions (Figure 2).
case_images: Light microscopy of lactophenol cotton blue slide preparations made of culture colonies revealed round sporangia containing spherical sporangiospores similar to those of P. wickerhamii.
case_images: CRIVEM2016-2878751.003.jpg
case_images: PMC6005278_01_CRIVEM2016-2878751.003.jpg
case_images: fig3
case_images: P. wickerhamii (Figure 3). P. zopfii cells are oval or cylindrical in shape, producing sporangia of larger diameter (15-25 mum) containing up to 20 sporangiospores.
case_images: Nodular-to-diffuse, pyogranulomatous panniculitis with numerous Prototheca-like elements stained vividly purple with PAS stain.
case_images: CRIVEM2016-2878751.004.jpg
case_images: PMC6005278_01_CRIVEM2016-2878751.004.jpg
case_images: fig4
case_images: Alignment of the sequence obtained in this case to the corresponding fragment of the GenBank number AB183198 sequence. Highlighted letters indicate the differences. The image was edited with Krita 2.8.5 (https://krita.org).
case_images: CRIVEM2016-2878751.005.jpg
case_images: PMC6005278_01_CRIVEM2016-2878751.005.jpg
case_images: fig5
case_images: Similar sequences were searched in the GenBank with the aid of the Web interface of Blast software which returned 9 of these sequences that belonged to Prototheca wickerhamii strains; the higher similarity applied to GenBank number AB183198 sequence (Figure 5).