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case_images: Minimal residual disease of AML1/ETO expression by quantitative RT-PCR assays. The expression level of fusion gene decreased from 407.55% at diagnosis to 0.00% (MRD negative) 19 months after allo-HSCT (cured).
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case_images: Subsequently, consolidation therapy was administered with HAD and intermediate-dose cytarabine, however, the patient did not reach genetic minimal residual disease (MRD) negativity throughout the treatment course (Figure 1).
case_images: The condition of fever and agranulocytosis during stem cell engraftment. The patient experienced persistent fever (A) during the whole stage of agranulocytic, and the infection got gradually controlled since day +10 when neutrophil engrafted (B).
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case_images: The patient's experienced persistent agranulocytosis and the condition deteriorated with persistent fever ranging from 39-40 C, despite aforementioned antibiotic treatment (Figure 2A and B).
case_images: Changes of the infected perianal skin. (A) showed perianal purulent spot formed on day +6. (B) showed perianal abscess with local rupture on day +8. (C) showed giant perianal ulcer after infection control on day +21. (D), (E) and (F) showed the recovery of perianal ulcer.
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case_images: Moreover, the perianal skin started rupturing and showed signs of suppuration (pus formation) (Figure 3).
case_images: The evidence of pathogenic bacterium and whole-genome sequencing (WGS) analysis. (A) showed the antimicrobial disk combined susceptibility tests. (B) showed Plasmid ring map of pE45, and (C) presented chromosome and plasmid straight lines.
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case_images: Antimicrobial disk combined susceptibility tests (Figure 4A) indicated that when combined with polymyxin B, there was a slight zone of inhibition observed, suggesting some degree of effectiveness against the strain. Results showed that the particular strain belonged to ST463 P. aeruginosa, and carried both the blaAFM-1 and blaKPC-2 genes (Figure 4B and C).