case_images: PMC11165237_01
case_images: Histopathological features of ETV6-NTRK3 rearranged thyroid cancer. Tumors typically exhibited a combination of papillary and follicular growth patterns. (A) Case 1: The tumor displayed noticeable nuclear enlargement, clearing, and irregularities in the cell membrane (H&E, x200). (B) Case 2: The tumor showed follicular epithelium hyperplasia with crowded nuclei (H&E, x200).
case_images: fonc-14-1332522-g001.jpg
case_images: PMC11165237_01_fonc-14-1332522-g001.jpg
case_images: f1
case_images: The tumor is composed of small- to normal-sized follicles and has nuclear features of papillary thyroid carcinoma showing enlarged nuclei, irregular nuclear membranes, and chromatin clearing and glassy nuclei ( Figure 1 ).
case_images: Staining profiles of CD56, CK19, Galectin-3, and HBME-1 in the two ETV6-NTRK3-rearranged FVPTC cases. (A, B) CD56 staining was consistently negative (100x). (C, D) CK19 staining was consistently positive (100x). (E, F) Galectin-3 exhibited strong diffuse staining in the cytoplasm and nucleus (100x). (G, H) HBME-1 staining showed sporadic positivity (100x).
case_images: fonc-14-1332522-g002.jpg
case_images: PMC11165237_01_fonc-14-1332522-g002.jpg
case_images: f2
case_images: Immunohistochemical staining profiles showed CD56-, CK19+, Galectin-3+, HBME1+ ( Figure 2 ).
case_images: (A) Both cases of FVPTC demonstrated ETV6::NTRK3 fusion with identical breakpoints, involving ETV6 exon 4 and NTRK3 exon 14. (B) FISH analysis using a dual-color break-apart NTRK3 probe (green signal for 5'NTRK3 and red signal for 3'NTRK3) confirmed NTRK3 rearrangement. (C) Immunohistochemistry (IHC) using a Pan-TRK antibody revealed weak cytoplasmic and nuclear staining in ETV6-NTRK3 rearranged thyroid cancer.
case_images: fonc-14-1332522-g003.jpg
case_images: PMC11165237_01_fonc-14-1332522-g003.jpg
case_images: f3
case_images: Additionally, Pan-TRK staining demonstrated positive results ( Figure 3 ).