case_images: PMC5253504_01
case_images: Molecular modeling of the L354P mutation reveals alteration of local structure leading to changes in flexibility. (a) The TGFBR2 kinase domain structure is shown in a blue cartoon representation with amino acid position 354 marked by a red sphere and an adenosine placed in the ATP binding pocket shown as ball-and-stick. Representatives from the beginning of our MD simulation are shown as white backbone trace. (b) Root Mean Squared Fluctuations (RMSF) capture the flexibility of the protein and are plotted for the mutant and wt simulations. Regions with largest difference are highlighted (colored similarly in (a)) and position 354 is marked with an asterisk. (c) During our simulation, the local helical structure around L354P is destabilized and unwinds, pushing the rest of the helix into an altered conformation.
case_images: CRIG2017-7263780.001.jpg
case_images: PMC5253504_01_CRIG2017-7263780.001.jpg
case_images: fig1
case_images: Receptor IIA (see Figure 1) using available structural information for other members of this family of serine/threonine kinases. Ramachandran plots identified 96.5% of the residues in the allowable regions, reflecting the high quality of our model. Schematic representation of the protein molecular model is shown in Figure 1.