case_images: PMC10867175_01
case_images: Photos from the dorsal (A) and lateral (B) views of the 3-day-old female Holstein calf with the protruding mass in its back, when taken after clipping on day 14. Cr: cranial; Cd: caudal. Lateral radiograph (C) showing the mass located on the level between the first and fourth lumbar vertebras (L1 and L4). The deformed spinous process is evident in the second and third lumbar vertebras (L2 and L3), compared with the radiopaque shapes of the spinous process in the thirteen thoracic vertebrae (T13), and the first, fourth, fifth and sixth lumbar vertebrae (L1, L4, L5, and L6). Cr: cranial; Cd: caudal.
case_images: fvets-10-1257329-g001.jpg
case_images: PMC10867175_01_fvets-10-1257329-g001.jpg
case_images: fig1
case_images: At that time, owner noticed formation of a large-sized swelling in its back locating at the level between thirteen thoracic and fourth lumbar vertebras (Figures 1A,B). Lateral radiography identified a large-sized, smooth mass located close and dorsally to the second and third lumbar vertebras (Figure 1C).
case_images: Doppler ultrasonograms (A-D) of the mass, when scanning by the cranial-caudal moving of a 10.0 MHz linear transducer. Anechoic bronchi-like structures (Br) and the large vessels with rich blood flow (V) run within the homogenous echogenic mass's parenchymas (M), separated by the hyperechoic septal structures. Ultrasonogram (E) and Doppler ultrasonogram (F) showing the bifurcation part of the large vessel (V) in the ventral margin of the protruding mass (M) at the levels of the lumbar vertebral body (VB), when scanning the right-sided base of the mass using a 3.5 MHz microconvex transducer. Bar: 1 cm.
case_images: fvets-10-1257329-g002.jpg
case_images: PMC10867175_01_fvets-10-1257329-g002.jpg
case_images: fig2
case_images: This revealed a larger 8-10-mm diameter vessel running within the homogenous echogenic parenchyma at approximately 3 cm under the skin's surface (Figures 2A-D). The large vessel could be visualized as running toward the body, when scanning the right-sided base of the mass using a 3.5 MHz microconvex transducer (Figures 2E,F).
case_images: Photos of the surgical procedure for resection of the back mass. (A) A dark-red mass (M) is exposed when the incised skin (S) is dissected bluntly. (B) The large, tortuous vessels (arrowhead) are evident on the surface of the mass (M). (C) Two braided silk sutures (SS) are set around the part of the vessel's entry (arrow) to the mass (M). (D) The part of the vessel's entry (arrow) to the mass (M) is sutured. (E) Resection of the mass is carried out, while the stalk-like structure of the mass's base is sutured with braided silk suture material (SS). Arrow shows the resected part. (F) The macroscopic views after skin suturing. Cr: Cranial; Cd: Caudal.
case_images: fvets-10-1257329-g003.jpg
case_images: PMC10867175_01_fvets-10-1257329-g003.jpg
case_images: fig3
case_images: Blunt dissection of the subcutaneous tissues soon allowed exposure of a dark-red surface of the mass (Figure 3A). On the surface of the mass, several 2-5-mm diameter tortuous vessels were macroscopically detected (Figure 3B). Ltd., Osaka, Japan) at positions proximal and distal to the planned incision part, as close to it as possible (Figures 3C,D). Three-time suturing-incision procedures could allow the mass resection at the location of 1-2 cm under the skin's surface (Figure 3E). Tokyo, Japan), followed by skin suturing (Figure 3F). The animal was treated with a five-day course of intramuscular penicillin-streptomycin mixed solution (Mycillin Sol, Tamura Pharmaceutical Co.
case_images: The cut surface of the resected mass (A) showing the bronchi-like structures (Br) and the large vessel (V) in the center of the parenchyma, including mixtures of hard and spongiform structures. (B) Histopathologically, the mass comprised of lung tissues, including bronchioles (Br) and collagenous tissues. Cartilage tissues (asterisks) locate around the bronchioles. HE. Bar, 500 mum. (C) Alveolus-like structures similar to pulmonary alveoli in a magnified view of the mass. Fibrous tissue similar to pleura is on the left upper of the photo. HE. (D) The compressed area is similar to atelectatic lung tissue. Arrows indicate hemosiderin-laden macrophages within the alveoli and bronchiole. HE. Bars are 50 mum in C and D.
case_images: fvets-10-1257329-g004.jpg
case_images: PMC10867175_01_fvets-10-1257329-g004.jpg
case_images: fig4
case_images: Macroscopically, the mass had a dark-red-colored, irregular outer surface, covering the internal structures entirely, when removed surgically on day 14 (Figure 4A). Histopathology of the resected mass contained the branching bronchi-like structures with a single layer of columnar ciliated epithelial cells (Figure 4B). Deposition of collagenous fibers, middle- to large-sized arteries, cartilage tissues and pulmonary alveoli-like structures were also found within the mass (Figure 4C). Congestion, hemorrhage, hemosiderin-laden macrophages, edema and fibrin deposition were also noted in these areas (Figure 4D).