case_images: PMC9719925_01
case_images: PMC9719925_02
case_images: (A) Photos of the ovary during ovarian tissue biopsy; (B) Ovarian tissue was transported to ovarian tissue cryobank for ovarian tissue preparation; (C) An antral follicle can be seen during ovarian tissue preparation; (D) Detection of follicular activity in ovarian cortex.
case_images: fendo-13-959912-g001.jpg
case_images: PMC9719925_02_fendo-13-959912-g001.jpg
case_images: f1
case_images: The whole right ovary was removed at Children's Hospital, Capital Institute of Pediatrics, on April 29th, 2022, and normal ovarian morphology could be observed ( Figure 1A ). The ovarian tissue was quickly placed in a low-temperature transfer solution in vitro and transported to our ovarian tissue cryobank within 1 hour ( Figure 1B ). During the preparation of ovarian tissue, the follicles reported by ultrasound were found ( Figure 1C ). The method of follicle counting was the same as the previously published paper, with an average of 1380 follicles in a diameter of 2 mm of the round cortical slice, and the follicle density was about 440/mm3 ( Figure 1D ).
case_images: (A, C) The typical pictures of HE staining of the ovarian cortex of this TS girl can see the different stages of follicles, most of which are primordial follicles; (B, D) The typical pictures of HE staining of non-TS girl. Bars: A, B = 50 mum, C, D = 20 mum.
case_images: fendo-13-959912-g002.jpg
case_images: PMC9719925_02_fendo-13-959912-g002.jpg
case_images: f2
case_images: TS girl can see the different stages of follicles, most of which are primordial ( Figures 2A, C ). The typical HE staining pictures of non-TS girls are shown in  Figures 2B, D .