case_images: PMC11090189_01
case_images: Flow chart of the development process and outcome of the case.
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case_images: A 71-year-old male patient was admitted on February 6, 2023, due to swelling of both lower limbs and abdominal distension for more than 20 days (Figure 1).
case_images: Microscopic morphological characteristics of smear staining (magnification: 1000x): Gram staining (A), Wright-Giemsa staining (B), Ziehl-Neelsen staining (C), modified Ziehl-Neelsen staining (D).
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case_images: PMC11090189_01_IDR-17-1803-g0002.jpg
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case_images: MacConkey agar plate (Yancheng, China), and placed the plates into a 35 C, 5% CO2 incubator. Meanwhile, smear staining and microscopic examination were conducted, and the results showed gram-positive coccobacillus (Figure 2A), negative for acid fast staining (Figure 2C), and partially positive for modified Ziehl-Neelsen staining (Figure 2D). Wright-Giemsa staining was conducted for a better observation (Figure 2B).
case_images: Different incubation time of the colony growth characteristics of G. terrae subculture on Columbia blood agar:24h (A), 48h (B), 72h (C), 120h (D).
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case_images: PMC11090189_01_IDR-17-1803-g0003.jpg
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case_images: Scattered pinpoint-shaped colonies emerged on the blood agar plate after 24 hours of cultivation (Figure 3A), and small white colonies appeared after 48 hours (Figure 3B). After 72 hours, dry, wrinkled, and protruding colonies were formed, which were light brown with irregular edges (Figure 3C). After 120 hours, the colonies gradually turned dark brown (Figure 3D).