case_images: PMC3916506_01
case_images: Preoperative view of discoloured maxillary lateral incisor.
case_images: rde-39-51-g001.jpg
case_images: PMC3916506_01_rde-39-51-g001.jpg
case_images: F1
case_images: A 35 year old male patient reported to the Department of Periodontics with a complaint of pain in the upper left front tooth region associated with pus discharge for 2 months (Figure 1).
case_images: Preoperative radiograph showing radiolucency along distal aspect of maxillary lateral incisor to the apex.
case_images: rde-39-51-g002.jpg
case_images: PMC3916506_01_rde-39-51-g002.jpg
case_images: F2
case_images: Intraoral periapical X-ray (IOPA) was taken which revealed radiolucency along the distal side of the entire root surface to the apex (Figure 2).
case_images: Separation of platelet rich plasma from the collected autologous blood.
case_images: rde-39-51-g003.jpg
case_images: PMC3916506_01_rde-39-51-g003.jpg
case_images: F3
case_images: Whole blood was initially centrifuged (3,000 rpm for 10 minutes, Figure 3).The plasma was then aspirated with a micropipette and subjected to a second centrifugation at 2,000 rpm for 10 minutes, which allowed the precipitation of the platelets (0.8 to 1.2 mL) to fall onto the bottom.
case_images: Platelet rich fibrin after activation with calcium chloride.
case_images: rde-39-51-g004.jpg
case_images: PMC3916506_01_rde-39-51-g004.jpg
case_images: F4
case_images: Coagulated preparation of 0.3 mL of PRF was obtained by its combination with 0.1 g of calcium chloride (Figure 4).
case_images: Curettage of the defect after reflection of soft tissue flap using intracrevicular incision.
case_images: rde-39-51-g005.jpg
case_images: PMC3916506_01_rde-39-51-g005.jpg
case_images: F5
case_images: A full thickness flap was raised at the labial aspect using intracrevicular incision (Figure 5).
case_images: Placement of graft into defect.
case_images: rde-39-51-g006.jpg
case_images: PMC3916506_01_rde-39-51-g006.jpg
case_images: F6
case_images: Thorough curettage of the defect area was done and a combination of PRF and bioactive glass was placed in the defect (Figures 6 and 7).
case_images: Placement of PRF.
case_images: rde-39-51-g007.jpg
case_images: PMC3916506_01_rde-39-51-g007.jpg
case_images: F7
case_images: Thorough curettage of the defect area was done and a combination of PRF and bioactive glass was placed in the defect (Figures 6 and 7).
case_images: Follow-up radiographs (a) at 6 months, postoperative view showing bone fill; (b) at 12 months.
case_images: rde-39-51-g008.jpg
case_images: PMC3916506_01_rde-39-51-g008.jpg
case_images: F8
case_images: PRF and bone graft resulted in substantial amount of bone fill (Figures 8a and 8b), and probing depth was reduced to 2 mm.