case_images: PMC10972885_01
case_images: A slightly stronger echo (red arrow) of 1.2 cmx0.9 cmx1.3 cm in size was found in the lower part of the uterine cavity, with a clear border and point-like blood flow signals peripheral and inward from the echo.
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case_images: Furthermore, ultrasound showed a mass in the lower segment of the uterine body and the junction of the cervix ( Figure 1 ).
case_images: Microscopic features. (A) The surface and glandular epithelium was benign, but the stroma was hyperplastic (HEx40). (B) Focally, hypercellular stromal cuffs were observed around glands (HEx100). (C, D) There were numerous bizarre stromal cells that were pleomorphism, multinucleate and hyperchromatic (HEx400).
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case_images: PMC10972885_01_fonc-14-1351646-g002.jpg
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case_images: Extraordinarily, there were numerous bizarre stromal cells that were pleomorphism, multinucleate and hyperchromatic ( Figure 2 ).
case_images: Immunohistochemistry. The stromal cells showed positive staining for CD10 (A), ER (B), cyclin-D1 (C) and P16 (D) but lacked staining for caldesmon (E). The Ki-67 index (F) was approximately 10%-20%.
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case_images: The Ki-67 index was approximately 10%-20% ( Figure 3 ).
case_images: Whole-exome sequencing (WES) detected 7 mutations in the tumor, including amplification of MDM2, CDK6, CDK4, KMT2D, HGF and GRM3 and rearrangement of KMT2D. The most significant mutation was MDM2 amplification.
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case_images: Figure 4 ). FISH (fluorescence in situ hybridization) was performed to verify the prominent amplification of MDM2 ( Figure 5 ).
case_images: FISH. Amplification of MDM2 showed clustered signals.
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case_images: PMC10972885_01_fonc-14-1351646-g005.jpg
case_images: f5
case_images: Figure 4 ). FISH (fluorescence in situ hybridization) was performed to verify the prominent amplification of MDM2 ( Figure 5 ).