case_images: PMC4323767_01
case_images: Taxonomical classification of 16S rDNA found in reported samples.. A and B: Tree view of 16S rDNA sequences from culture-positive urine (alpha-hemolytic Streptococcus >105 CFU/ml), assigned at different taxonomy levels (family, genus and species) as computed by MEGAN 3.4 for V1V2 (A) and V6 (B) amplicons. Each circle represents a taxon and is labeled by its name and the number of sequenced reads assigned. Taxonomical assignment was done by comparing the sequences to a curated version of the SSUrdp database, then assigning the sequences to the taxon of the best-matched reference sequence (lowest common ancestor). The size of the circles is scaled logarithmically to the number of reads assigned to the taxon.. C and D: Comparison of bacterial communities from culture-positive urine positive for alpha-hemolytic green Streptococcus (red bars) and culture-negative urine (blue bars) from a case subject diagnosed with overactive bladder syndrome. Sequence analysis is based on V1V2 (C) and V6 (D) amplicons. Only major genera with a sequence abundance >=10 % for either V1V2 or V6 analysis are shown. The calculation of sequence abundance for each genus was relative to the total number of sequence assigned to Bacteria in each dataset.
case_images: TOMICROJ-8-148_F1.jpg
case_images: PMC4323767_01_TOMICROJ-8-148_F1.jpg
case_images: F1
case_images: In contrast to the clinical reports, the molecular analysis revealed a complex bacterial composition of the urine sample taken (Fig. 1A, 1B). However, the previously dominant genus Atopobium was completely absent this time (Fig. 1C, 1D).