case_images: PMC8762299_01
case_images: Preoperative images of the renal artery. (A): CT renal angiography; (B): CT 3D reconstruction of renal vessels.
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case_images: PMC8762299_01_fbioe-09-738434-g001.jpg
case_images: F1
case_images: Renal artery CTA showed 80% stenosis in the proximal segment of the left renal artery and 90% stenosis in the initial segment of the right renal artery ( Figures 1A,B ).
case_images: 3D printing cold jacket of the kidney.
case_images: fbioe-09-738434-g002.jpg
case_images: PMC8762299_01_fbioe-09-738434-g002.jpg
case_images: F2
case_images: The printing time is 8 hours; after the printing, the cold jacket is rinsed in 70% ethanol overnight and sterilized for 90 min using the low-temperature plasma hydrogen peroxide sterilizer (Shandong Xinhua Medical Instrument Co., LTD., SQ-D, China) (Figure 2).
case_images: (A) Intracorporeal cold perfusion of the kidney using a cannula and tubing passed through the assistant port to allow renal artery perfusion until clear effluent was seen from the renal vein and the color of the right kidney was pale; (B) 3D-printed cold jacket was completely covered on the surface of the free kidney.
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case_images: PMC8762299_01_fbioe-09-738434-g003.jpg
case_images: F3
case_images: Immediately after dividing the vessels, the perfusion cannula was inserted in the transected artery lumen, which was continuously flushed with ice-cold perfusion liquid (hypertonic purine citrate fluid) solution under gravity until the clear effluent was seen from the renal vein and the color of the right kidney was pale (Figure 3A). The kidney was carefully placed in the pelvis, and the opening of the renal artery and vein were placed close to the external iliac vessels (Figure 3B; Figure 4).
case_images: 3D-printed model of the cold jacket covered on the surface of free kidney.
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case_images: PMC8762299_01_fbioe-09-738434-g004.jpg
case_images: F4
case_images: The kidney was carefully placed in the pelvis, and the opening of the renal artery and vein were placed close to the external iliac vessels (Figure 3B; Figure 4).