case_images: PMC8301612_01
case_images: PMC8301612_02
case_images: Minimum spanning tree based on the cgMLST typing of the Norwegian VanD VREfm.. Regardless of the different STs, A1-3 strains clustered together while the VREfm strain B1 showed 354 allelic differences with the A2 strain.
case_images: pone.0255187.g001.jpg
case_images: PMC8301612_02_pone.0255187.g001.jpg
case_images: pone.0255187.g001
case_images: This was further shown by cgMLST analysis which revealed that A1-3 strains belonged to the same novel cluster type (CT) 3198 (Fig 1).
case_images: Phylogenetic SNP tree of the vanD gene clusters of the Norwegian and vanD1:vanD5 subtype reference clusters retrieved from NCBI.. Flags represent the countries that vanD-types were discovered in first. Case A strains clustered with vanD5 reference N03-0072 while case B strains clustered separately.
case_images: pone.0255187.g002.jpg
case_images: PMC8301612_02_pone.0255187.g002.jpg
case_images: pone.0255187.g002
case_images: A clustered with the vanD5 reference sequence (E. faecium strain N03-0072) (Fig 2). ANIs between the vanD5 reference sequence and A1-3 strains were >99.9%.