case_images: PMC10612393_01
case_images: A) Ocular melanoma before surgical resection associated with electrochemotherapy; B) Application of electrochemotherapy in the surgical site; C) Immediate post-surgical appearance
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case_images: PMC10612393_01_vrf-14-575-g001.jpg
case_images: F1
case_images: However, the left eye had mucoid secretion, conjunctival hyperemia, and a pedunculated irregular nodular neoformation with a blackish appearance in the superficial limbal-conjunctival region with corneal involvement measuring approximately 8.00 x 7.00 x 7.00 mm (Fig. 1A), without presenting intra-ocular alterations assessed by pupillary dilation with 10.00 mg mL-1 tropicamide (Alcon, Geneva, Switzerland), gonioscopy (Volk, Mentor, USA) and ocular ultrasound (Siemens, Munich, Germany).
case_images: Photomicrograph of a canine ocular melanoma indicating the presence of fusiform neoplastic cells with oval nuclei and moderate, eosinophilic, and distinct cytoplasm, being variably filled with coarse and black granulation (circle). Nuclei show moderate atypia (arrowhead; elongated nuclei) with chromatin being coarsely aggregated and condensed on the periphery of the nuclear membrane, having evident and central nucleoli (arrow). Binucleation figures are also observed. The presence of intense pigmentation makes it difficult to count mitoses, (Hematoxylin and Eosin staining, 20x)
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case_images: F2
case_images: Later, the histopathological examination using light microscopy confirmed that the lesion was melanoma (Fig. 2).
case_images: A) Ten days after surgical treatment with adjuvant electrochemotherapy of the canine ocular melanoma. No signs of recurrence and presence of cicatricial granuloma are found; B) Presence of conjunctivalization 25 days after treatment. C) 45 days after the initial treatment of the canine ocular melanoma, there was a > 95.00% reduction in conjunctivalization and no signs of tumor recurrence were seen; D) 105 days after the initial treatment of the canine ocular melanoma, conjunctivalization was completely healed and there were no signs of inflammation or tumor recurrence. A slight peripheral corneal opacification was also observed. E) 215 days after the initial treatment of the canine ocular melanoma, no signs of inflammation or tumor recurrence were detected. The total resolution of the corneal opacification was also observed
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case_images: PMC10612393_01_vrf-14-575-g003.jpg
case_images: F3
case_images: The absence of tumor recurrence signs was observed (Fig. 3A). Twenty-five days after the initial treatment, the patient presented granuloma formation and inflammatory epithelial hyperplasia at the surgical site (Fig. 3B), absence of corneal lesions on examination with fluorescein and intra-ocular pressure of 19.00 mmHg. After 105 days (Fig. 3D), the conjunctivalization proved to be completely healed, and there were no signs of inflammation or tumor recurrence. A complete resolution of corneal opacification was also observed (Fig. 3E).