case_images: PMC3898211_01
case_images: Capillary zone electrophoresis of the patient's hemoglobin. CE shows the hemoglobin variant eluting with normal hemoglobins. Note peaks corresponding to Hb Shepherds Bush (6.5% of total, zone 10), HbA (89.6%, zone 9), HbF (0.3%, zone 7), HbA2 (3.2%, zone 3) and hemoglobin breakdown products (0.4%, zone 2). This chromatogram was obtained after a transfusion following a parvovirus infection described later accounting for the small amount of her native hemoglobin compared to HbA.
case_images: 1472-6890-14-4-1.jpg
case_images: PMC3898211_01_1472-6890-14-4-1.jpg
case_images: F1
case_images: A CE chromatogram performed at a later date after transfusion (Figure 1) is included to show the migration pattern of our patient's hemoglobin variant, which eluted slightly earlier than HbA.
case_images: High performance liquid chromatography analysis of the patient's hemoglobin. HPLC analysis shows a peak of unknown variety at 2.13 min that eluted earlier than HbA at 2.44 min. The chromatogram also contains peaks corresponding to HbF and HbA2.
case_images: 1472-6890-14-4-2.jpg
case_images: PMC3898211_01_1472-6890-14-4-2.jpg
case_images: F2
case_images: Using high performance liquid chromatography (HPLC) for hemoglobin (Figure 2), several hemoglobin types were demonstrated.
case_images: Alkaline hemoglobin electrophoresis of the patient's hemoglobin. Alkaline hemoglobin electrophoresis on cellulose acetate with densitometer tracing superimposed below electrophoresis bands shows a dense band where HbA is expected to appear and a second band that has migrated more anodally indicating an unknown hemoglobin variant.
case_images: 1472-6890-14-4-3.jpg
case_images: PMC3898211_01_1472-6890-14-4-3.jpg
case_images: F3
case_images: Alkaline gel electrophoresis was also utilized to demonstrate the migration of the unknown hemoglobin variant, which migrated slightly more anodally than HbA (Figure 3).