case_images: PMC5477902_01
case_images: Admixtures of the affected patient serum with other bicarbonate sources yield predicted bicarbonate levels. Aliquots of affected serum (7.7 mEq/L) were mixed with either a bicarbonate solution (31.9 mEq/L) or a normal serum (25.6 mEq/L) at the indicated percentages. Measured bicarbonate levels in the admixtures were plotted together with the predicted bicarbonate values in the admixtures calculated from the sum of the individual component contributions.
case_images: sfm032fig1.jpg
case_images: PMC5477902_01_sfm032fig1.jpg
case_images: F1
case_images: Recovery of bicarbonate was at least 85-90% at all dilutions (Figure 1). As shown in Figure 2, a positive bias of 1.7 mEq/L was observed.
case_images: Bland-Altman plot of the difference between calculated bicarbonate and enzymatic measurement of serum bicarbonate. Data represent a series of 88 paired patient data sets of calculated bicarbonate, derived from the Henderson-Hasselbalch equation applied to arterial blood gas parameters and a matched serum bicarbonate measurement. Units are mEq/L. Solid line represents the mean difference or bias of 1.7 mEq/L. Several data points are superimposed.
case_images: sfm032fig2.jpg
case_images: PMC5477902_01_sfm032fig2.jpg
case_images: F2
case_images: As shown in Figure 2, a positive bias of 1.7 mEq/L was observed.