case_images: PMC11769397_01
case_images: PMC11769397_02
case_images: Gross lesions of a respiratory disease killed the foal: (A) A respiratory disease in foal donkey; (B) Gross change in lungs; (C) Hyperemia and hemorrhage in lung; (D) Severe interstitial pneumonia.
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case_images: vetsci-12-00056-f001
case_images: The clinical signs, including anorexia, high fever, nasal discharge, dyspnea, and depression, were also observed in infected foals (Figure 1A). Necropsy of one deceased foal revealed a blue/purple discoloration of the lung tissue, with no significant gross abnormalities noted (Figure 1B). Histopathological examination of the lung showed hyperemia and hemorrhage of the alveolar septa (Figure 1C), as well as thickening of the alveolar septa and a notable increase in inflammatory cell infiltration (Figure 1D).
case_images: Screening of virus pathogens. Viral DNA/RNA was extracted from different samples: (A) EAV; (B) H3N8 M; (C) H3N8 HA; (D) EHV-1; (E) EHV-4; (F) EHV-8. They were detected by RT/PCR and PCR. Lane M represents a 5000 bp DNA molecular weight ladder. Moreover, 1 represents negative control, 2 represents nose swabs, and 3 represents lung of donkey foal.
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case_images: The immunohistochemistry (IHC) detection for EHV-1 in the lung of the donkey. The IHC was performed to detect the EHV-1 antigen in the lungs. The experimental group was treated with mouse anti-EHV-1 positive serum on the lung (A). The normal mouse serum-treated group served as a negative control on lung (B). Scale bars, 50 mum.
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case_images: Positive staining for EHV-1 antigen was observed in the lung tissue, with brown-colored signals indicating the presence of the virus, as detected using mouse anti-EHV-1 serum (Figure 3A). No such positive signals were observed in the negative control section (Figure 3B).