case_images: PMC5424191_01
case_images: (a) Peripheral blood promyelocytes; (b) peripheral blood pseudo-Pelger-Huet neutrophils; (c) bone marrow effacement by promyelocytes at diagnosis.
case_images: CRIHEM2017-7657393.001.jpg
case_images: PMC5424191_01_CRIHEM2017-7657393.001.jpg
case_images: fig1
case_images: Peripheral blood promyelocytes, which accounted for 40% of nucleated cells, had regular nuclei, were hypergranular, and lacked Auer rods (Figure 1(a)) with increased numbers of pseudo-Pelger-Huet neutrophils noted (Figure 1(b)). The bone marrow was effaced by promyelocytes (Figure 1(c)) which stained strongly positive for Sudan Black.
case_images: Sanger sequencing demonstrating fusion of ZBTB16 exon 3 to RARA exon 2.
case_images: CRIHEM2017-7657393.002.jpg
case_images: PMC5424191_01_CRIHEM2017-7657393.002.jpg
case_images: fig2
case_images: A standardised reverse transcription-PCR approach did not detect PML-RARA transcripts, but, using primers previously described, a ZBTB16-RARA fusion transcript, consistent with fusion of ZBTB16 exon 3 fused to RARA exon 2, was detected and confirmed by Sanger sequencing (Figure 2).