case_images: PMC3021853_01
case_images: Case 1: Radiography at the first visit showing radiolucency at the apex area of tooth 46.
case_images: IJD2010-942130.002.jpg
case_images: PMC3021853_01_IJD2010-942130.002.jpg
case_images: fig2
case_images: Deep caries were detected in tooth 25, and radiolucency was detected in the periapical lesion (Figure 2).
case_images: Case 1: Result of a chairside anaerobic culture test at the first visit showing thick bacterial lines.
case_images: IJD2010-942130.003.jpg
case_images: PMC3021853_01_IJD2010-942130.003.jpg
case_images: fig3
case_images: Minimal pus was exuded from the root canal, but the chairside anaerobic culture test revealed large amounts of bacteria in the root canal; solid lines of bacterial colonies were detected from the top to the bottom of the culture plate (Figure 3).
case_images: Case 1: Result of a chairside anaerobic culture test at the second visit showing thin and dotted bacterial lines.
case_images: IJD2010-942130.004.jpg
case_images: PMC3021853_01_IJD2010-942130.004.jpg
case_images: fig4
case_images: The anaerobic culture test showed some solid lines followed by dotted lines, indicating that the bacterial load was decreasing compared to the first visit (Figure 4).
case_images: Case 1: Radiography of the root canal filling showing remaining bone loss at the root apex.
case_images: IJD2010-942130.005.jpg
case_images: PMC3021853_01_IJD2010-942130.005.jpg
case_images: fig5
case_images: At the fourth visit (22nd day), the root canal was filled with gutta-percha points and root canal sealer (Canals, Showa Yakuhin Kako Co., Ltd., Tokyo, Japan) with a lateral condensation method (Figure 5).
case_images: Case 1: Radiography 2 months after the initial visit showing that the radiolucency at the apex had decreased.
case_images: IJD2010-942130.006.jpg
case_images: PMC3021853_01_IJD2010-942130.006.jpg
case_images: fig6
case_images: Two months after the initial visit, the radiolucency was diminishing (Figure 6).
case_images: PMC3021853_02
case_images: Case 2: Radiography at the first visit showing radiolucency at the apex area of tooth 46.
case_images: IJD2010-942130.007.jpg
case_images: PMC3021853_02_IJD2010-942130.007.jpg
case_images: fig7
case_images: Deep caries were present in tooth 46, and a large radiolucency was observed in the periapical lesion (Figure 7).
case_images: Case 2: Result of a chairside anaerobic culture test at the third visit showing several kinds of bacterial colonies including black-pigmented bacteria.
case_images: IJD2010-942130.008.jpg
case_images: PMC3021853_02_IJD2010-942130.008.jpg
case_images: fig8
case_images: At the third visit (12th day), pain had completely disappeared, but many different types of colonies remained on the culture plate (Figure 8), including black-pigmented bacteria (BPB), which indicated a mixed infection of the root canal.
case_images: Case 2: Result of a chairside anaerobic culture test at the fourth visit showing scattered bacterial colonies.
case_images: IJD2010-942130.009.jpg
case_images: PMC3021853_02_IJD2010-942130.009.jpg
case_images: fig9
case_images: Pus discharge was not observed at the fourth visit (19th day), and the quantity of bacteria had decreased at this time (Figure 9).
case_images: Case 2: Radiography at the root canal filling showing remaining bone loss at the root apex.
case_images: IJD2010-942130.010.jpg
case_images: PMC3021853_02_IJD2010-942130.010.jpg
case_images: fig10
case_images: When the absence of endodontic bacteria was confirmed at the fifth visit (26th day), the root canal was filled with gutta-percha points as described above (Figure 10). Radiolucency was still observed at the apex of the distal root (Figure 10).
case_images: Radiography 10 weeks after the initial visit showing that the radiolucency at the apex had decreased.
case_images: IJD2010-942130.011.jpg
case_images: PMC3021853_02_IJD2010-942130.011.jpg
case_images: fig11
case_images: The radiolucency was improving 10 weeks after the initial visit (Figure 11).