case_images: PMC10634489_01
case_images: Phenotypic detection of the inducible chromosomal ampC gene via a disc approximation test.
case_images: acmi-5-571.v4-g001.jpg
case_images: PMC10634489_01_acmi-5-571.v4-g001.jpg
case_images: F1
case_images: PCR for the genes CMY/MOX, CIT, DHA, ACC, ACT/MIR and FOX, as described by Perez-Perez FJ and Hanson 2002, to determine carriage of chromosomally mediated ampC genes, was also negative. Phenotypic detection of a chromosomally encoded ampC gene in the presence of a potent inducer of ampC (cefoxtin) revealed a positive result as indicated by a flattening of the zone of inhibition around cefotaxime 30 microg when placed close to the inducer 30 microg cefoxitin (disc approximation test) (Fig. 1).