case_images: PMC11095243_01
case_images: Microphotographs of Plasmodium ovale in field-stained thin and thick blood smears. Magnification 1000X showing thin (a-c) and thick (d-f) blood smears obtained from the patient under study: (a) ring; (b,c,d) young trophozoite; (e) late trophozoite; (f) gametocyte.
case_images: 1678-9946-rimtsp-66-S1678-9946202466029-gf01.jpg
case_images: PMC11095243_01_1678-9946-rimtsp-66-S1678-9946202466029-gf01.jpg
case_images: f01
case_images: Species-specific PCR for Plasmodium: (a) PCR strategy; (b-f) agarose gels (2%) stained with ethidium bromide. PCR reaction with primers specific for P. falciparum (b), P. vivax (c), P. malariae (d), P. ovale curtisi (e), and P. ovale wallikeri (f). DNA from P. falciparum (P. f), P. vivax (P. v) and P. malariae (P. m) was used as a control in the PCR assays. Neg = Negative control (reaction without template); Case = DNA from the blood sample of the patient under study; M = Molecular weight marker (GeneRuler 100 bp Plus DNA Ladder. Thermo). The white stars indicate the PCR product obtained from the first PCR test (this 1200 pb amplicon is shared by Plasmodium sp. and was used as a template in the species-specific PCR). The black stars indicate the expected size for species-specific PCR products.
case_images: 1678-9946-rimtsp-66-S1678-9946202466029-gf02.jpg
case_images: PMC11095243_01_1678-9946-rimtsp-66-S1678-9946202466029-gf02.jpg
case_images: f02
case_images: PCR reaction is then performed with primers unique to P. falciparum (rFAL1 and rFAL2), P. vivax (rVIV1 and rVIV2), P. malariae (rMAL1 and rMAL2), or P. ovale (rOVA1 and rOVA2) (Figure 2a). PCR was performed according to the conditions described by Cortes and Guerra in 2020  . Although the patient's sample was positive in the first reaction, indicating the presence of Plasmodium, no amplification for any of the four species was obtained in the second PCR (Figure 2b-2e).