case_images: PMC4168750_01
case_images: Primary clear cell carcinoma of the thyroid.. The neoplastic cells have a small, peripheral nucleus, with abundant clear cytoplasm. Nests of carcinomatous elements are separated by a fibrous vascularized stroma. Hematoxylin and eosin staining, 5x.
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case_images: PMC4168750_01_f1000research-3-4503-g0000.jpg
case_images: f1
case_images: On microscopic examination, nests of carcinomatous elements separated by a fibrous vascularized stroma were observed ( Figure 1, hematoxylin and eosin).
case_images: Lung metastases.. Nuclear staining for TTF1 is strongly positive, 20x.
case_images: f1000research-3-4503-g0001.jpg
case_images: PMC4168750_01_f1000research-3-4503-g0001.jpg
case_images: f2
case_images: Thyroid Transcription Factor 1 (TTF-1) ( Figure 2), confirming as a result that the organ primarily affected was indeed the thyroid. There were no metastases in other organs, and the kidneys, as seen previously, were free of tumour.
case_images: FLCN sequences.. The c.1062G>C wild type allele in exon 9 is lost in the tumour, while the patient is heterozygote for the mutation in the germline. Top sequence, control DNA. Middle sequence, germline DNA (normal tissue). Bottom sequence, tumour DNA.
case_images: f1000research-3-4503-g0002.jpg
case_images: PMC4168750_01_f1000research-3-4503-g0002.jpg
case_images: f3
case_images: The c.1062G>C wild type allele was lost in the tumour, strongly suggesting loss of heterozygosity (LOH) ( Figure 3).