case_images: PMC9380824_01
case_images: Head and lung CT scans of the patient after admission in our hospital. (A). Head CT showing a small amount of blood, effusion, and swelling of the surrounding soft tissue in the basal ganglia after intracerebral hemorrhage surgery. (B). Lung CT showing no obvious signs of infection.
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case_images: PMC9380824_01_IDR-15-4479-g0001.jpg
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case_images: Head CT after admission showed a small amount of blood and fluid accumulation in the operative area after intracerebral hemorrhage (Figure 1A), while lung CT showed no obvious signs of infection (Figure 1B).
case_images: mNGS analysis reveals the mapping and distribution of M. hominis reads. (A) Mapping of M. hominis reads on the genome with a coverage of 3.7%. (B) (left) Total read distribution in the CSF sample, and (right) read distribution without the human host.
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case_images: PMC9380824_01_IDR-15-4479-g0002.jpg
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case_images: A total of 1424 reads were identified to correspond to the M. hominis genome, with a coverage of 3.7% (Figure 2A and B).
case_images: A 399-bp target fragment mapping to M. hominis, identified by sequence-specific PCR. Lane M: DNA ladder; Lane 1: negative control; Lane 2: annealing temperature of 58  C; Lanes 3 and 4: annealing temperature of 60  C.
case_images: IDR-15-4479-g0003.jpg
case_images: PMC9380824_01_IDR-15-4479-g0003.jpg
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case_images: The results suggested a 399-bp target fragment aligned with the reference sequences of M. hominis (GenBank: CP055144.1) with 99.73% identity, thereby indicating M. hominis infection in this patient (Figure 3).