case_images: PMC11420620_01
case_images: Cytologic features from a fine needle aspirate of thickened perianal tissue in a boxer mix canine with basidiobolomycosis. (A) Several deeply basophilic hyphal bodies or chlamydospores. Wright-Giemsa, 100x. (B) Basophilic conidia with lateral papillae. Wright-Giemsa, 100x.
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case_images: fig1
case_images: Similar structures, consistent with conidia, occasionally contained lateral papillae (Figure 1).
case_images: Gross and microscopic features of colorectal basidiobolomycosis in a boxer mix canine. (A) Rectum and colon on cut section. The rectum and most aborad colon are circumferentially thickened. (B) Photomicrograph of rectum demonstrates areas of thickening are the result of pyogranulomatous inflammation centered on numerous fungal organisms that often form 25-45 mum in diameter zygospores with a thick eosinophilic wall (arrows) H & E. Scale bar = 75 mum. (C) Fungal organisms are strongly Periodic Acid Schiff (PAS) positive (arrows). Scale bar = 75 mum.
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case_images: fig2
case_images: On postmortem examination, perianal soft tissue and rectum were markedly thickened up to 5.0 cm circumferentially by multifocal to coalescing, tan-white, firm nodules (Figure 2A). On histopathology from the distal colon and rectum, there was severe, chronic-active, regionally extensive pyogranulomatous colitis and proctitis with intralesional Gomori Methenamine Silver (GMS) negative (Figure 2B), Periodic acid-Schiff (PAS) positive (Figure 2C) fungal hyphae and hyphal bodies or chlamydospores.
case_images: Basidiobolus arizonensis.
  (A) White to cream, rugose colony on Sabouraud dextrose agar, 7d, 25 C; microscopic images from a 6d slide culture at 25 C on PFA: (B) distal portion of elongating hypha at margin of growing mycelia. (C,D) Encounter of opposite sex hyphae before the formation of lateral beaks (white arrows). (E,F) After the exchange of genetic material, zygospores develop with their characteristic beak. (G,H) Globose conidia (black arrow) bearing a uninucleated adhesive conidium (white arrow). (I) Detached adhesive conidium. (J) Adhesive conidium converted to sporangium releasing globose sporangiospores. (K) Germinating zygospores.
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case_images: fig3
case_images: The wet mount and slide culture preparations showed morphological characteristics consistent with Basidiobolus by the production of zygospores with paired protuberances or lateral beaks leading to a structure known as a "beaked zygospore" (Figure 3). Sanders sp. nov. (Figure 3). Description: on SDA, 7d at 25 C, colonies grew 24-28 mm x 21 mm-27 mm in diameter, white to cream, glabrous, rugose and radially folded towards the rim (Figure 3A). Hyphal elements hyaline, coenocytic, occasionally septate (Figure 3E). Globose conidium, 18.6 mm - 18.78 mm diameter (black arrow) bearing adhesive conidium (white arrow) (Figures 3G,H). Encounter of opposite sex hyphae before the formation of lateral beaks (Figures 3C,D, white arrows). Mature zygospores, 21.4 mm - 27.88 mm in diameter with paired protuberances ready to germinate (Figures 3E,F,K). Detached adhesive conidium 14.94 mm-34.33 mm (L) x 12.27 mm-22.31 mm (W) (Figure 3I). Adhesive conidium converted to sporangium releasing globose sporangiospores, 7.2 mm-10.39 mm diameter (Figure 3J). Germinating zygospores (Figure 3K).
case_images: Maximum likelihood phylogram generated from combined ITS and partial LSU sequences of UTHSCSA DI22-113 and representative Basidiobolus spp. Numbers on the nodes are (SH-aLRT) >= 80%; (aBayes) >= 0.95; (UFBoot) >= 75%. The new species proposed is indicated in bold blue. T = ex-types of strains. The tree is rooted to Mucor janssenii CBS 205.68T.
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case_images: fig4
case_images: BLASTn results and phylogenetic analyses of individual (not shown) and combined ITS and LSU sequences using the backbone tree of Al-Hatmi et al., showed that UTHSCSA DI22-113 is closely related to but phylogenetically distant from the recognized species B. meristosporus and B. omanensis (Figure 4).