case_images: PMC5100078_01
case_images: Surgical resection of the tumor. Tumor of 35 x 30 x 17 mm
case_images: 12610_2016_41_Fig1_HTML.jpg
case_images: PMC5100078_01_12610_2016_41_Fig1_HTML.jpg
case_images: Fig1
case_images: Photo of the tumor at low magnification (X50). Nodular and well limited tumor composed of sheets of eosinophilic cells with Hematoxylin Eosin and Saffron (HES) stain
case_images: 12610_2016_41_Fig2_HTML.jpg
case_images: PMC5100078_01_12610_2016_41_Fig2_HTML.jpg
case_images: Fig2
case_images: A (Ventana, prediluted, pretreatment: EDTA pH 7.8 for 60 min) and to SALL4 (Sigma, Dilution: 1/1500, pretreatment: EDTA pH 7.8 for 60 min). All tumor cells were diffusely and strongly stained with antibody to Inhibin A corresponding to Leydig cell tumor and were completely negative to antibodies to SALL4 showing that there was no germ cell contingent (Figs. 2, 3 and 4).   
case_images: Photo of the tumor at high magnification (X200). Tumor cells are large with an abundant eosinophilic cytoplasm and round regular nuclei with small nucleoli, according with Leydig cells. Hematoxylin Eosin and Saffron (HES) stain
case_images: 12610_2016_41_Fig3_HTML.jpg
case_images: PMC5100078_01_12610_2016_41_Fig3_HTML.jpg
case_images: Fig3
case_images: A (Ventana, prediluted, pretreatment: EDTA pH 7.8 for 60 min) and to SALL4 (Sigma, Dilution: 1/1500, pretreatment: EDTA pH 7.8 for 60 min). All tumor cells were diffusely and strongly stained with antibody to Inhibin A corresponding to Leydig cell tumor and were completely negative to antibodies to SALL4 showing that there was no germ cell contingent (Figs. 2, 3 and 4).   
case_images: Photo of the tumor at high magnification (X400). Tumors cells were diffusely stained with antibody to inhibin A(immunoperoxydase). All tumor cells present a diffuse and strong cytoplasmic staining
case_images: 12610_2016_41_Fig4_HTML.jpg
case_images: PMC5100078_01_12610_2016_41_Fig4_HTML.jpg
case_images: Fig4
case_images: A (Ventana, prediluted, pretreatment: EDTA pH 7.8 for 60 min) and to SALL4 (Sigma, Dilution: 1/1500, pretreatment: EDTA pH 7.8 for 60 min). All tumor cells were diffusely and strongly stained with antibody to Inhibin A corresponding to Leydig cell tumor and were completely negative to antibodies to SALL4 showing that there was no germ cell contingent (Figs. 2, 3 and 4).   
case_images: Photo of the testicular biopsy at high magnification (X100). Histopathological micrograph with hematoxylin-eosin-green FCF stain. The testicular biopsy consists of tubules with hypospermatogenesis (white arrows) mixed with aplasia (black arrows)
case_images: 12610_2016_41_Fig5_HTML.jpg
case_images: PMC5100078_01_12610_2016_41_Fig5_HTML.jpg
case_images: Fig5
case_images: Testicular Steroidogenesis
case_images: 12610_2016_41_Fig6_HTML.jpg
case_images: PMC5100078_01_12610_2016_41_Fig6_HTML.jpg
case_images: Fig6