case_images: PMC10521202_01
case_images: Numerous budding yeasts in mucoid & necrotic background [Haematoxylin & Eosin x 100].
case_images: TurkPatolojiDerg-39-12421-g001.jpg
case_images: PMC10521202_01_TurkPatolojiDerg-39-12421-g001.jpg
case_images: F44777101
case_images: Formalin fixed paraffin embedded hematoxylin & eosin-stained sections showed necrotic and mucoid areas with mixed inflammatory cell infiltration including neutrophils, lymphocytes, and histiocytes (Figure 1). They showed 'single' narrow based budding (Figure 1, Figure 2) as well as 'multiple' narrow based budding including 'sequential budding' resulting in "chains of yeasts" (Figure 3 and Figure 4) and multiple 'non sequential budding (Figure 3 and Figure 4).
case_images: Numerous budding yeasts in mucoid & necrotic background [Gomori's methenamine silver nitrate stain x 100].
case_images: TurkPatolojiDerg-39-12421-g002.jpg
case_images: PMC10521202_01_TurkPatolojiDerg-39-12421-g002.jpg
case_images: F89739281
case_images: They showed 'single' narrow based budding (Figure 1, Figure 2) as well as 'multiple' narrow based budding including 'sequential budding' resulting in "chains of yeasts" (Figure 3 and Figure 4) and multiple 'non sequential budding (Figure 3 and Figure 4). The organisms were GMS (Gomori's methenamine silver nitrate stain) positive (Figure 2, Figure 4).
case_images: H & E. [Fig A, x 40; Fig B, C, D, x 100; Fig E, F, G x 200]. Numerous thick walled 'narrow based' budding yeasts in a mucoid to necrotic background, many of which show 'tube like isthmus' connecting parent cell with daughter cells [3E, short black arrow head]. Many of these yeasts show 'retained tube' after the detachment from parent cell [3B&C, short black arrow head]. They also showed characteristic multiple 'sequential' budding resulting in 'chain of yeasts' [3A, C, D &E, long black arrow heads] and multiple irregular 'non sequential' budding [3G, short black arrow head].
case_images: TurkPatolojiDerg-39-12421-g003.jpg
case_images: PMC10521202_01_TurkPatolojiDerg-39-12421-g003.jpg
case_images: F45863721
case_images: They showed 'single' narrow based budding (Figure 1, Figure 2) as well as 'multiple' narrow based budding including 'sequential budding' resulting in "chains of yeasts" (Figure 3 and Figure 4) and multiple 'non sequential budding (Figure 3 and Figure 4). Characteristically, the parent cell and daughter cells were connected by 'narrow stalk' or "tube like isthmus" (also called 'connecting tubes') (Figure 3 and Figure 4). Also, detached cells showed 'retained tube like structures' on one cell (Figure 3 and Figure 4) and 'bud scars' on the other (Figure 4). 'Tube like structures' were also observed in yeasts with multiple 'non sequential budding' (Figure 4).
case_images: Gomori's methenamine silver nitrate stain. [Fig A, B, C, E, F, G, I, J, L x 100; Fig D, H, K, M x 200]. Yeasts showed characteristic multiple 'sequential' budding resulting in 'chain of yeasts' [4A, B, C, D, F & G, long black arrow heads] and multiple irregular 'non sequential' budding [4E, I & L, short black arrow head]. Many of which show 'tube-like isthmus' connecting parent cell with daughter cells [4E, short black arrow head] and 'retained tube' after the detachment from parent cell [4J&M, short black arrow head]. They also showed characteristic 'bud scars' [4K, long black arrow heads
case_images: TurkPatolojiDerg-39-12421-g004.jpg
case_images: PMC10521202_01_TurkPatolojiDerg-39-12421-g004.jpg
case_images: F41781051
case_images: They showed 'single' narrow based budding (Figure 1, Figure 2) as well as 'multiple' narrow based budding including 'sequential budding' resulting in "chains of yeasts" (Figure 3 and Figure 4) and multiple 'non sequential budding (Figure 3 and Figure 4). Characteristically, the parent cell and daughter cells were connected by 'narrow stalk' or "tube like isthmus" (also called 'connecting tubes') (Figure 3 and Figure 4). Also, detached cells showed 'retained tube like structures' on one cell (Figure 3 and Figure 4) and 'bud scars' on the other (Figure 4). 'Tube like structures' were also observed in yeasts with multiple 'non sequential budding' (Figure 4). Occasionally, budding yeasts showed "hour glass appearance" due to elongation of connecting tubes (Figure 4). The organisms were GMS (Gomori's methenamine silver nitrate stain) positive (Figure 2, Figure 4).