Published January 30, 2025 | Version v1
Lesson Open

Image handling using Fiji - training materials

Creators

  • 1. ROR icon Åbo Akademi University

Description

Description:
This training package provides a guide to image processing and analysis using ImageJ/Fiji, an open-source software widely used in biological and medical imaging. The manual includes step-by-step exercises demonstrating practical workflows for measuring size distribution and signal intensity using both conventional thresholding and advanced tools like StarDist. This resource is ideal for researchers, students, and professionals looking to enhance their image analysis skills using Fiji.

Key topics include:

  • Image calibration and intensity adjustments
  • Channel splitting and merging
  • Projection techniques and scale bar addition
  • Segmentation and thresholding methods
  • Quantitative analysis of nuclei and fluorescence signal intensity

Publication Date: January 2025

Keywords: Fiji, ImageJ, Image Analysis, Microscopy, Segmentation, Particle Analysis, 3D Visualization, StarDist

License: MIT

Files

20x nucleus_high contrast.tif

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