Published January 5, 2025 | Version v1
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Detección, cuantificación y caracterización rápida de microplásticos: Comparación de dos métodos

Creators

  • 1. Instituto Politécnico Nacional, Reynosa

Description

La contaminación por microplásticos es un problema que afecta la salud humana y los ecosistemas. Estos diminutos fragmentos plásticos se acumulan en los tejidos de los organismos y pueden ingresar a la cadena alimentaria, llegando hasta el ser humano. Este estudio se centró en desarrollar, optimizar y comparar 2 métodos para detectar y cuantificar micropartículas de plástico en tejidos sintéticos y de algodón. Se emplearon tratamientos químicos y filtración para aislar las partículas, y se utilizó una cámara de Neubauer y la microscopia de fluorescencia para su visualización y conteo. Los resultados mostraron que la combinación de agua oxigenada y calor fue la más efectiva para purificar y visualizar las partículas, mientras que el sulfato ferroso dificultó su detección debido a la formación de flóculos. Estos hallazgos son relevantes para futuras investigaciones y para el desarrollo de estrategias de monitoreo y mitigación de la contaminación por microplásticos.

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Dates

Accepted
2025-01-04

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https://produccioncientificaluz.org/index.php/rluz/article/view/43190
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References

  • Al-Azzawi, M.S.M.; Kefer, S.; Weißer, J.; Reichel, J.; Schwaller, C.; Glas, K.; Knoop, O.; Drewes, J.E. (2020). Validation of Sample Preparation Methods for Microplastic Analysis in Wastewater Matrices—Reproducibility and Standardization. Water, 12, 2445, pp. 1-27. https://doi.org/10.3390/w12092445
  • Andrady, A. L. (2010, November). Measurement and Occurrence of Microplastics in the Environment. In Presentation at the 2nd research workshop on microplastic debris. Tacoma, WA.
  • Aytan, U., Esensoy, F. B., & Senturk, Y. (2022). Microplastic ingestion and egestion by copepods in the Black Sea. The Science of the total environment, 806(Pt 4), 150921, pp. 1-10. https://doi.org/10.1016/j.scitotenv.2021.150921
  • Bajt, O. (2021). From plastics to microplastics and organisms. In FEBS Open Bio (Vol. 11, Issue 4, pp. 954–966. John Wiley and Sons Inc. https://doi.org/10.1002/2211-5463.13120
  • Blackburn, K., & Green, D. (2022). The potential effects of microplastics on human health: What is known and what is unknown. In Ambio (Vol. 51, Issue 3, pp. 518–530). Springer Science and Business Media B.V. https://doi.org/10.1007/s13280-021-01589-9
  • Bollaín Pastor, Clara, & Vicente Agulló, David. (2019). Presencia de microplásticos en aguas y su potencial impacto en la salud pública. Revista Española de Salud Pública, 93, e201908064, pp. 1-10. Epub 07 de septiembre de 2020. Recuperado en 04 de octubre de 2024, de https://www.scielosp.org/article/resp/2019.v93/e201908064/es/#
  • Campanale, C., Massarelli, C., Savino, I., Locaputo, V., & Uricchio, V. F. (2020). A detailed review study on potential effects of microplastics and additives of concern on human health. In International Journal of Environmental Research and Public Health (Vol. 17, Issue 4) pp. 1-26. MDPI AG. https://doi.org/10.3390/ijerph17041212
  • Capparelli, M. V., Molinero, J., Moulatlet, G. M., Barrado, M., Prado-Alcívar, S., Cabrera, M., Gimiliani, G., Ñacato, C., Pinos-Velez, V., & Cipriani-Avila, I. (2021). Microplastics in rivers and coastal waters of the province of Esmeraldas, Ecuador. Marine Pollution Bulletin, 173, pp. 1-6. https://doi.org/10.1016/j.marpolbul.2021.113067
  • Courtene-Jones, W., Quinn, B., Gary, S. F., Mogg, A. O. M., & Narayanaswamy, B. E. (2017). Microplastic pollution identified in deep-sea water and ingested by benthic invertebrates in the Rockall Trough, North Atlantic Ocean. Environmental Pollution, 231, 271–280. https://doi.org/10.1016/J.ENVPOL.2017.08.026
  • De Falco, F., Di Pace, E., Cocca, M., & Avella, M. (2019). The contribution of washing processes of synthetic clothes to microplastic pollution. Scientific Reports, 9(1), pp. 1-11. https://doi.org/10.1038/s41598-019-43023-x
  • Dehaut, A., Cassone, A. L., Frère, L., Hermabessiere, L., Himber, C., Rinnert, E., Rivière, G., Lambert, C., Soudant, P., Huvet, A., Duflos, G., & Paul-Pont, I. (2016). Microplastics in seafood: Benchmark protocol for their extraction and characterization. Environmental Pollution, 215, 223–233. https://doi.org/10.1016/J.ENVPOL.2016.05.018
  • Egessa, R., Nankabirwa, A., Ocaya, H., & Pabire, W. G. (2020). Microplastic pollution in surface water of Lake Victoria. Science of the Total Environment, 741, pp. 1-9. https://doi.org/10.1016/j.scitotenv.2020.140201
  • Fowler, S. D., & Greenspan, P. (1985). Application of Nile red, a fluorescent hydrophobic probe, for the detection of neutral lipid deposits in tissue sections: comparison with oil red O. The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 33(8), 833–836. https://doi.org/10.1177/33.8.4020099
  • Geyer, R., Jambeck, J. R., & Law, K. L. (2017). Production, use, and fate of all plastics ever made. Science advances, 3(7), e1700782, pp.1-5. https://doi.org/10.1126/sciadv.1700782
  • Greenspan, P., Mayer, E. P., & Fowler, S. D. (1985). Nile red: a selective fluorescent stain for intracellular lipid droplets. The Journal of cell biology, 100(3), 965–973. https://doi.org/10.1083/jcb.100.3.965
  • González, Y. (2019). Mejor sin plástico: guía para llevar una vida sostenible. Plataforma Editorial 4.ª ed. ISBN: 978-84-17622-16-9
  • Hirt, N., & Body-Malapel, M. (2020). Immunotoxicity and intestinal effects of nano- and microplastics: a review of the literature. In Particle and Fibre Toxicology (Vol. 17, Issue 1), BioMed Central Ltd, pp. 1-27.. https://doi.org/10.1186/s12989-020-00387-7
  • Issac, M. N., & Kandasubramanian, B. (2021). Effect of microplastics in water and aquatic systems. Environmental science and pollution research international, 28(16), 19544–19562. https://doi.org/10.1007/s11356-021-13184-2
  • Kannan, K., & Vimalkumar, K. (2021). A Review of Human Exposure to Microplastics and Insights Into Microplastics as Obesogens. Frontiers in endocrinology, 12, 724989, pp. 1-19. https://doi.org/10.3389/fendo.2021.724989
  • Koelmans, A. A., Mohamed Nor, N. H., Hermsen, E., Kooi, M., Mintenig, S. M., & De France, J. (2019). Microplastics in freshwaters and drinking water: Critical review and assessment of data quality. In Water Research (Vol. 155, pp. 410–422). Elsevier Ltd. https://doi.org/10.1016/j.watres.2019.02.054
  • Lenaker, P. L., Baldwin, A. K., Corsi, S. R., Mason, S. A., Reneau, P. C., & Scott, J. W. (2019). Vertical Distribution of Microplastics in the Water Column and Surficial Sediment from the Milwaukee River Basin to Lake Michigan. Environmental Science and Technology, 53(21), 12227–12237. https://doi.org/10.1021/acs.est.9b03850
  • Lv, L., Qu, J., Yu, Z., Chen, D., Zhou, C., Hong, P., Sun, S., & Li, C. (2019). A simple method for detecting and quantifying microplastics utilizing fluorescent dyes - Safranine T, fluorescein isophosphate, Nile red based on thermal expansion and contraction property. Environmental Pollution (1987), 255(Pt 2), 113283–113283. https://doi.org/10.1016/j.envpol.2019.113283
  • Maes, T., Jessop, R., Wellner, N., Haupt, K., & Mayes, A.G. (2017). A rapid-screening approach to detect and quantify microplastics based on fluorescent tagging with Nile Red. Scientific Reports, 7, pp. 1-10. DOI: https://doi.org/10.1038/srep44501
  • Mai, L., Bao, L. J., Shi, L., Wong, C. S., & Zeng, E. Y. (2018). A review of methods for measuring microplastics in aquatic environments. In Environmental Science and Pollution Research (Vol. 25, Issue 12, pp. 11319–11332). Springer Verlag. https://doi.org/10.1007/s11356-018-1692-0
  • Martinez, V., & Henary, M. (2016). Nile Red and Nile Blue: Applications and Syntheses of Structural Analogues. Chemistry (Weinheim an der Bergstrasse, Germany), 22(39), 13764–13782. https://doi.org/10.1002/chem.201601570
  • Munno, K., Helm, P. A., Jackson, D. A., Rochman, C., & Sims, A. (2018). Impacts of temperature and selected chemical digestion methods on microplastic particles. Environmental Toxicology and Chemistry, 37(1), 91–98. https://doi.org/10.1002/etc.3935
  • Prata, J. C., Reis, V., Matos, J. T. V., da Costa, J. P., Duarte, A. C., & Rocha-Santos, T. (2019). A new approach for routine quantification of microplastics using Nile Red and automated software (MP-VAT). The Science of the total environment, 690, 1277–1283. https://doi.org/10.1016/j.scitotenv.2019.07.060
  • Prata, J. C., da Costa, J. P., Duarte, A. C., & Rocha-Santos, T. (2019). Methods for sampling and detection of microplastics in water and sediment: A critical review. In TrAC - Trends in Analytical Chemistry (Vol. 110, pp. 150–159). Elsevier B.V. https://doi.org/10.1016/j.trac.2018.10.029
  • Prata, J. C., da Costa, J. P., Lopes, I., Duarte, A. C., & Rocha-Santos, T. (2020). Environmental exposure to microplastics: An overview on possible human health effects. In Science of the Total Environment (Vol. 702). Elsevier B.V., pp. 1-9. https://doi.org/10.1016/j.scitotenv.2019.134455
  • Qiu, Q., Peng, J., Yu, X., Chen, F., Wang, J., & Dong, F. (2015). Occurrence of microplastics in the coastal marine environment: First observation on sediment of China. Marine Pollution Bulletin, 98(1–2), 274–280. https://doi.org/10.1016/J.MARPOLBUL.2015.07.028
  • Rhodes, C. J. (2018). Plastic pollution and potential solutions. Science Progress, 101(3), 207–260. https://doi.org/10.3184/003685018X15294876706211
  • Šaravanja, A., Pušić, T., & Dekanić, T. (2022). Microplastics in Wastewater by Washing Polyester Fabrics. In Materials (Vol. 15, Issue 7). MDPI., pp. 1-15. https://doi.org/10.3390/ma15072683
  • Schrank, I., Möller, J. N., Imhof, H. K., Hauenstein, O., Zielke, F., Agarwal, S., Löder, M. G. J., Greiner, A., & Laforsch, C. (2022). Microplastic sample purification methods - Assessing detrimental effects of purification procedures on specific plastic types. The Science of the total environment, 833, 154824, pp. 1-13. https://doi.org/10.1016/j.scitotenv.2022.154824
  • Shoopman, C. H., & Pan, X. (2021). Microplastics: A Review of Methodology for Sampling and Characterizing Environmental and Biological Samples. In Methods in Molecular Biology (Vol. 2326, pp. 339–359). Humana Press Inc. https://doi.org/10.1007/978-1-0716-1514-0_25
  • Shruti, V. C., Pérez-Guevara, F., Roy, P. D., & Kutralam-Muniasamy, G. (2022). Analyzing microplastics with Nile Red: Emerging trends, challenges, and prospects. Journal of hazardous materials, 423(Pt B), 127171, pp. 1-19. https://doi.org/10.1016/j.jhazmat.2021.127171
  • Singh, R. P., Mishra, S., & Das, A. P. (2020). Synthetic microfibers: Pollution toxicity and remediation. In Chemosphere (Vol. 257). Elsevier Ltd., pp. 1-17. https://doi.org/10.1016/j.chemosphere.2020.127199
  • Talbot, R., & Chang, H. (2022). Microplastics in freshwater: A global review of factors affecting spatial and temporal variations. In Environmental Pollution (Vol. 292). Elsevier Ltd., pp. 1-11. https://doi.org/10.1016/j.envpol.2021.118393
  • Tirkey, A., & Upadhyay, L. S. B. (2021). Microplastics: An overview on separation, identification and characterization of microplastics. Marine Pollution Bulletin, 170, 112604, pp. 1-12. https://doi.org/10.1016/J.MARPOLBUL.2021.112604
  • Wang, Y., Zhou, B., Chen, H., Yuan, R., & Wang, F. (2022). Distribution, biological effects and biofilms of microplastics in freshwater systems - A review. In Chemosphere (Vol. 299). Elsevier Ltd., pp. 1-14. https://doi.org/10.1016/j.chemosphere.2022.134370
  • Wu, P., Huang, J., Zheng, Y., Yang, Y., Zhang, Y., He, F., Chen, H., Quan, G., Yan, J., Li, T., & Gao, B. (2019). Environmental occurrences, fate, and impacts of microplastics. In Ecotoxicology and Environmental Safety (Vol. 184). Academic Press., pp. 1-16. https://doi.org/10.1016/j.ecoenv.2019.109612
  • Xu, S., Ma, J., Ji, R., Pan, K., & Miao, A. J. (2020). Microplastics in aquatic environments: Occurrence, accumulation, and biological effects. In Science of the Total Environment (Vol. 703). Elsevier B.V., pp. 1-14. https://doi.org/10.1016/j.scitotenv.2019.134699
  • Yao, L., Hui, L., Yang, Z., Chen, X., & Xiao, A. (2020). Freshwater microplastics pollution: Detecting and visualizing emerging trends based on Citespace II. In Chemosphere (Vol. 245). Elsevier Ltd., pp. 1-8. https://doi.org/10.1016/j.chemosphere.2019.125627
  • Yu, X., Zhang, Y., Tan, L., Han, C., Li, H., Zhai, L., Ma, W., Li, C., & Lu, X. (2022). Microplastisphere may induce the enrichment of antibiotic resistance genes on microplastics in aquatic environments: A review. In Environmental Pollution (Vol. 310). Elsevier Ltd., pp. 1-8. https://doi.org/10.1016/j.envpol.2022.119891
  • Zambrano, M. C., Pawlak, J. J., Daystar, J., Ankeny, M., Cheng, J. J., & Venditti, R. A. (2019). Microfibers generated from the laundering of cotton, rayon and polyester based fabrics and their aquatic biodegradation. Marine Pollution Bulletin, 142, 394–407. https://doi.org/10.1016/J.MARPOLBUL.2019.02.062
  • Zhang, Q., Liu, T., Liu, L., Fan, Y., Rao, W., Zheng, J., & Qian, X. (2021). Distribution and sedimentation of microplastics in Taihu Lake. Science of the Total Environment, 795, pp. 1-11. https://doi.org/10.1016/j.scitotenv.2021.148745