Published January 13, 2017 | Version v1
Conference paper Open

CMOS-based Image Cytometry for Detection of Phytoplankton in Ballast Water

  • 1. ICFO – Institut de Ciencies Fotoniques, The Barcelona Institute of Sciences and Technology
  • 2. LABAQUA
  • 3. MemTeq Ventures Ltd
  • 4. Marine Eco Analytics
  • 5. ICREA – Institució Catalana de Recerca

Description

An image cytometer (CYT) for the analysis of phytoplankton in fresh and marine water environments is introduced. A linear quantification of the number of cells over several orders of magnitude of concentrations was observed using cultures of Tetraselmis and Nannochloropsis measured by autofluorescence of the chlorophyll in a laboratory environment. The functionality of the system outside the laboratory was analysed by phytoplankton quantification of samples taken from marine water environment (Dutch Wadden Sea, The Netherlands) and fresh water environment (Lake Ijssel, The Netherlands). The CYT was also employed to study the effects of two ballast water treatment systems (BWTS), based on chlorine electrolysis and UV sterilisation by determining the vitality of the phytoplankton. In order to ensure the detection limit, a large volume (1l) of samples was collected and concentrated to 3 ml using CelltrapTM filters. The results were compared to benchmarked flow cytometer and PAM Fluorometry at Marine Eco-Analytics (MEA-NL). The image cytometer reached a 10 cells/ml limit of detection (LoD) with an accuracy between 0.7 and 0.5 log, and a correlation of 88.29% in quantification and 96.21% in vitality, when compared to benchmarked monitoring techniques.

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References

  • Bax N, Williamson A, Aguero M, Gonzalez E and W Geeves. 2003. Marine invasive alien species: a threat to global biodiversity. Mar. Policy, 27: 313-323. https://doi.org/10.1016/s0308-597x(03)00041-1
  • Brussaard CPD, Peperzak L and JR Meer. 2016. Immediate Ecotoxicological Effects of Short-lived Oil Spills on Marine Biota. Nature Comm. 7. https://doi.org/10.1038/ncomms11206
  • Delacroix S, Vogelsang C, Tobiesen A and H Liltved. 2013. Disinfection by-products and ecotoxicity of ballast water after oxidative treatment – Results and experiences from seven years of full-scale testing of ballast water management systems. Mar. Poll. Bull. 73(1). https://doi.org/10.1016/j.marpolbul.2013.06.014
  • Dunstan PK and NJ Bax. 2008. Management of an invasive marine species: defining and testing the effectiveness of ballast-water management options using management strategy evaluation. ICES J. Mar. Sci. 65: 841-850. https://doi.org/10.1093/icesjms/fsn069
  • Fernandes JA et al. 2015. Costs and benefits to European shipping of ballast-water and hull-fouling treatment: Impacts of native and non-indigenous species. Mar. Pol. 64:148-155. 2015. https://doi.org/10.1016/j.marpol.2015.11.015
  • Golden JP, Hashemi N, Erickson JS and FS Ligler. 2012. A microflow cytometer for optical analysis of phytoplankton. SPIE Proc. 8212: 82120G-82120G-6. https://doi.org/10.1117/12.905679
  • Kannan V. 2015. Globalization and government regulations: Invasive species management in an era of interdependence. J. Crit. Writ. 10, 8-12: 3808a. Liltved H, Tobiessen A, Delacroix A and I Tryland. 2011. Filtration and UV treatment for ships' ballast water management – water quality challenges and UV-dose requirements. IUVA News, 13, 1.
  • Maranda L, Cox AM, Campbell RG and DC Smith. 2013. Chlorine dioxide as a treatment for ballast water to control invasive species: Shipboard testing", Mar. Pol. Bull. 75: 76-89. https://doi.org/10.1016/j.marpolbul.2013.08.002
  • Meneely JP, Campbell K, Greef C, Lochhead MJ and CT Elliott. 2013. Development and validation of an ultrasensitive fluorescence planar waveguide biosensor for the detection of paralytic shellfish toxins in marine algae", Biosens. Bioelectron. 41: 691-697 https://doi.org/10.1016/j.bios.2012.09.043
  • Moreno-Andrés J, Romero-Martínez L, Acevedo-Merino A and E Nebot, 2016. Determining disinfection efficiency on E. faecalis in saltwater by photolysis of H2O2: Implications for ballast water treatment. J. Chem. Eng., 283: 1339-1348. https://doi.org/10.1016/j.cej.2015.08.079
  • O'Connell MA, Belanger BA and PD Haaland. 1993. Calibration and assay development using the four-parameter logistic model. Chemom. Intell. Lab. Syst. 20: 97-114. https://doi.org/10.1016/0169-7439(93)80008-6
  • Pérez JM, Jofre M, Martínez P, Yáñez MA, Catalan V and V Pruneri. 2015. An image cytometer based on angular spatial frequency processing and its validation for rapid detection and quantification of waterborne bacteria. Analyst, vol. 140: 7734-7741. https://doi.org/10.1039/c5an01338k
  • Rivas-Hermann R, Köhler J and AE Scheepens. 2015. Innovation in product and services in the shipping retrofit industry: a case study of ballast water treatment systems. J. Clean Prod. 106: 443-454. 2015. https://doi.org/10.1016/j.jclepro.2014.06.062
  • Sanchez-Ferandin S, Leroy F, Bouget FY and F Joux. 2013. A new, sensitive marine microalgal recombinant biosensor using luminescence monitoring for toxicity testing of antifouling biocides. Appl. Environ. Microbiol. 79: 631-638. https://doi.org/10.1128/aem.02688-12
  • Schreiber U. 1998. Chlorophyll fluorescence: new instruments for special applications. 4253-4258. https://doi.org/10.1007/978-94-011-3953-3_984
  • Seebens H, Gastner MT and B Blasius. 2013. The risk of marine bioinvasion caused by global shipping. Ecol. Lett. 16: 782-900. https://doi.org/10.1111/ele.12111
  • Steuhouwer PP, Buma A and L Peperzak. 2015. A comparison of six different ballast water treatment systems based on UV radiation, electrochlorination and chlorine dioxide. Env. Tech. 36(16):1-30. https://doi.org/10.1080/09593330.2015.1021858
  • Veldhuis MJW and GW Kraay. 2000. Application of flow cytometry in marine phytoplankton research: current applications and future perspectives. Sci. Mar. 64: 121-134. https://doi.org/10.3989/scimar.2000.64n2121
  • Veldhuis MJ, Fuhr F, Boon JP and CC ten Hallers-Tjabbers. 2006. Treatment of Ballast Water; How to Test a System with a Modular Concept? Environ Technol., 27: 909-921. https://doi.org/10.1080/09593332708618701
  • Wollschläger J, Nicolaus A, Wiltshire KH and K Metfies. 2014. Assessment of North Sea phytoplankton via molecular sensing: A method evaluation. J. Plankton Res. 36: 695-708. https://doi.org/10.1093/plankt/fbu003