Figure_1
Figure_2
Figure_3
Figure_4
Figure_5
Figure_S1
Figure_S2
Figure_S3
Figure_S4
Figure_S5
Figure_S6
file_list.txt
file_list_1.txt
README.md
Figure_1\Figure_1A
Figure_1\Figure_1B_SDS-PAGE_Coomassie
Figure_1\Figure_1C_SEC_cGAS
Figure_1\Figure_1D_Mass_photometry_cGAS
Figure_1\Figure_1E_nanoDSF_cGAS
Figure_1\Figure_1F_TLC
Figure_1\Figure_1G_TLC_titration
Figure_1\Fig_1_preview.png
Figure_1\readme_Fig_1.md
Figure_1\Figure_1A\Figure_1A_cGAS_17bp.pdb
Figure_1\Figure_1A\readme_Fig_1A.md
Figure_1\Figure_1B_SDS-PAGE_Coomassie\Figure_1B_preview_annotated.png
Figure_1\Figure_1B_SDS-PAGE_Coomassie\Figure_1B_SDS-PAGE_Coomassie.tif
Figure_1\Figure_1B_SDS-PAGE_Coomassie\readme_Fig_1B.md
Figure_1\Figure_1C_SEC_cGAS\Figure_1C_SEC_cGAS.csv
Figure_1\Figure_1C_SEC_cGAS\readme_Fig_1C.md
Figure_1\Figure_1D_Mass_photometry_cGAS\Figure_1D_mass_photometry_0_raw_data.csv
Figure_1\Figure_1D_Mass_photometry_cGAS\Figure_1D_mass_photometry_1_histogram_data.csv
Figure_1\Figure_1D_Mass_photometry_cGAS\Figure_1D_mass_photometry_2_fitted_gaussian_data.csv
Figure_1\Figure_1D_Mass_photometry_cGAS\readme_Fig_1D.md
Figure_1\Figure_1E_nanoDSF_cGAS\Figure_1E_nanoDSF_cGAS_Ratio.csv
Figure_1\Figure_1E_nanoDSF_cGAS\Figure_1E_nanoDSF_cGAS_Ratio_derivative.csv
Figure_1\Figure_1E_nanoDSF_cGAS\readme_Fig_1E.md
Figure_1\Figure_1F_TLC\Figure_1F_TLC_plate.tif
Figure_1\Figure_1F_TLC\Figure_1F_TLC_plate_line_profile.csv
Figure_1\Figure_1F_TLC\readme_Fig_1F.md
Figure_1\Figure_1G_TLC_titration\Figure_1G_1_TLC_plate1_raw.tif
Figure_1\Figure_1G_TLC_titration\Figure_1G_1_TLC_plate2_raw.tif
Figure_1\Figure_1G_TLC_titration\Figure_1G_2_TLC_plate1_bgSubstracted.tif
Figure_1\Figure_1G_TLC_titration\Figure_1G_2_TLC_plate2_bgSubstracted.tif
Figure_1\Figure_1G_TLC_titration\Figure_1G_3_TLC_densitometry_plotted.csv
Figure_1\Figure_1G_TLC_titration\readme_Fig_1G.md
Figure_2\Figure_2A_MST_traces.csv
Figure_2\Figure_2B_dose-response_data.csv
Figure_2\Figure_2B_dose-response_data_fitted_parameters.csv
Figure_2\Figure_2B_dose-response_data_fit_values.csv
Figure_2\Fig_2_preview.png
Figure_2\readme_Fig_2.md
Figure_3\Figure_3A_microscopy
Figure_3\Figure_3B_microscopy
Figure_3\Figure_3C_TLC
Figure_3\Figure_3D_FRAP_pN
Figure_3\Figure_3E_FRAP_N
Figure_3\Fig_3_preview.png
Figure_3\readme_Fig_3.md
Figure_3\Figure_3A_microscopy\N_10uM.tif
Figure_3\Figure_3A_microscopy\N_10uM_cGAS_1uM.tif
Figure_3\Figure_3A_microscopy\pN_10uM.tif
Figure_3\Figure_3A_microscopy\pN_10uM_cGAS_1uM.tif
Figure_3\Figure_3A_microscopy\readme_Fig_3A.md
Figure_3\Figure_3B_microscopy\Figure_3B_Line_Profiles.csv
Figure_3\Figure_3B_microscopy\N_10uM_cGAS_1uM_dsDNA_05uM.tif
Figure_3\Figure_3B_microscopy\N_10uM_dsDNA_05uM.tif
Figure_3\Figure_3B_microscopy\pN_10uM_cGAS_1uM_dsDNA_05uM.tif
Figure_3\Figure_3B_microscopy\pN_10uM_dsDNA_05uM.tif
Figure_3\Figure_3B_microscopy\readme_Fig_3B.md
Figure_3\Figure_3C_TLC\Figure_3C_TLC_densitometry.csv
Figure_3\Figure_3C_TLC\Figure_3C_TLC_plate.tif
Figure_3\Figure_3C_TLC\readme_Fig_3C.md
Figure_3\Figure_3D_FRAP_pN\Figure_3D_FRAP_analysis_Intensities_normalized.csv
Figure_3\Figure_3D_FRAP_pN\Figure_3D_FRAP_analysis_Intensities_normalized_fitted.csv
Figure_3\Figure_3D_FRAP_pN\Figure_3D_FRAP_analysis_parameters_fitted.csv
Figure_3\Figure_3D_FRAP_pN\pN_drop_1_prebleach_frame3.tif
Figure_3\Figure_3D_FRAP_pN\pN_drop_2_bleach_frame4.tif
Figure_3\Figure_3D_FRAP_pN\pN_drop_3_recovered_frame143.tif
Figure_3\Figure_3D_FRAP_pN\pN_drop_FRAP_time_series.tif
Figure_3\Figure_3D_FRAP_pN\Raw_data
Figure_3\Figure_3D_FRAP_pN\readme_Fig_3D.md
Figure_3\Figure_3D_FRAP_pN\Raw_data\Figure_3D_FRAP_time_series_images1.tif
Figure_3\Figure_3D_FRAP_pN\Raw_data\Figure_3D_FRAP_time_series_images2.tif
Figure_3\Figure_3D_FRAP_pN\Raw_data\Figure_3D_FRAP_time_series_images3.tif
Figure_3\Figure_3E_FRAP_N\Figure_3E_FRAP_analysis_Intensities_normalized.csv
Figure_3\Figure_3E_FRAP_N\Figure_3E_FRAP_analysis_Intensities_normalized_fitted.csv
Figure_3\Figure_3E_FRAP_N\Figure_3E_FRAP_analysis_parameters_fitted.csv
Figure_3\Figure_3E_FRAP_N\N_drop_1_prebleach_frame3.tif
Figure_3\Figure_3E_FRAP_N\N_drop_2_bleach_frame4.tif
Figure_3\Figure_3E_FRAP_N\N_drop_3_recovered_frame143.tif
Figure_3\Figure_3E_FRAP_N\N_drop_FRAP_time_series.tif
Figure_3\Figure_3E_FRAP_N\Raw_data
Figure_3\Figure_3E_FRAP_N\readme_Fig_3E.md
Figure_3\Figure_3E_FRAP_N\Raw_data\Figure_3E_FRAP_time_series_images1.tif
Figure_3\Figure_3E_FRAP_N\Raw_data\Figure_3E_FRAP_time_series_images2.tif
Figure_4\Figure_4B_step1_association
Figure_4\Figure_4B_step2_dissociation
Figure_4\Fig_4_preview.png
Figure_4\readme_Fig_4.md
Figure_4\Figure_4B_step1_association\Figure_4B_step1_association_cGAS-H647_10nM_0_scan.tif
Figure_4\Figure_4B_step1_association\Figure_4B_step1_association_cGAS-H647_10nM_1_kymograph.tif
Figure_4\Figure_4B_step1_association\Figure_4B_step1_association_cGAS-H647_10nM_3_Time_IntensityMean.csv
Figure_4\Figure_4B_step2_dissociation\Figure_4B_step2_dissociation_cGAS-H647_10nM_0_scan.tif
Figure_4\Figure_4B_step2_dissociation\Figure_4B_step2_dissociation_cGAS-H647_10nM_1_kymograph.tif
Figure_4\Figure_4B_step2_dissociation\Figure_4B_step2_dissociation_cGAS-H647_10nM_3_Pixel_IntensityMean.csv
Figure_4\Figure_4B_step2_dissociation\Figure_4B_step2_dissociation_cGAS-H647_10nM_3_Time_IntensityMean.csv
Figure_5\Figure_5C_scans_kymographs_pN
Figure_5\Figure_5D_scans_kymographs_N
Figure_5\Fig_5_preview.png
Figure_5\readme_Fig_5.md
Figure_5\Figure_5C_scans_kymographs_pN\Figure_5C_Intensities_pN.csv
Figure_5\Figure_5C_scans_kymographs_pN\kymograph_step1_pN-GFP_100nM_cGAS-H646_10nM.tif
Figure_5\Figure_5C_scans_kymographs_pN\kymograph_step2_pN-GFP_100nM_cGAS-H646_10nM.tif
Figure_5\Figure_5C_scans_kymographs_pN\kymograph_step3_pN-GFP_100nM_cGAS-H646_10nM.tif
Figure_5\Figure_5C_scans_kymographs_pN\kymograph_step4_pN-GFP_100nM_cGAS-H646_10nM.tif
Figure_5\Figure_5C_scans_kymographs_pN\Raw_data_confocal_scans
Figure_5\Figure_5C_scans_kymographs_pN\Raw_data_confocal_scans\step1_pN-GFP_100nM_cGAS-H646_10nM.tif
Figure_5\Figure_5C_scans_kymographs_pN\Raw_data_confocal_scans\step2_pN-GFP_100nM_cGAS-H646_10nM.tif
Figure_5\Figure_5C_scans_kymographs_pN\Raw_data_confocal_scans\step3_pN-GFP_100nM_cGAS-H646_10nM.tif
Figure_5\Figure_5C_scans_kymographs_pN\Raw_data_confocal_scans\step4_pN-GFP_100nM_cGAS-H646_10nM.tif
Figure_5\Figure_5D_scans_kymographs_N\Figure_5D_Intensities_N.csv
Figure_5\Figure_5D_scans_kymographs_N\kymograph_step1_N-GFP_100nM_cGAS-H646_10nM.tif
Figure_5\Figure_5D_scans_kymographs_N\kymograph_step2_N-GFP_100nM_cGAS-H646_10nM.tif
Figure_5\Figure_5D_scans_kymographs_N\kymograph_step3_N-GFP_100nM_cGAS-H646_10nM.tif
Figure_5\Figure_5D_scans_kymographs_N\kymograph_step4_N-GFP_100nM_cGAS-H646_10nM.tif
Figure_5\Figure_5D_scans_kymographs_N\Raw_data_confocal_scans
Figure_5\Figure_5D_scans_kymographs_N\Raw_data_confocal_scans\step1_N-GFP_100nM_cGAS-H646_10nM.tif
Figure_5\Figure_5D_scans_kymographs_N\Raw_data_confocal_scans\step2_N-GFP_100nM_cGAS-H646_10nM.tif
Figure_5\Figure_5D_scans_kymographs_N\Raw_data_confocal_scans\step3_N-GFP_100nM_cGAS-H646_10nM.tif
Figure_5\Figure_5D_scans_kymographs_N\Raw_data_confocal_scans\step4_N-GFP_100nM_cGAS-H646_10nM.tif
Figure_S1\Figure_S1A
Figure_S1\Figure_S1B
Figure_S1\Figure_S1C
Figure_S1\Figure_S1D
Figure_S1\Figure_S1E
Figure_S1\Figure_S1F
Figure_S1\Figure_S1G
Figure_S1\Fig_S1_preview.png
Figure_S1\readme_Fig_S1.md
Figure_S1\Figure_S1A\Figure_S1A_SDS-PAGE.tif
Figure_S1\Figure_S1A\readme_Fig_S1A.md
Figure_S1\Figure_S1B\Figure_S1B_1_Heparin_chromatography.csv
Figure_S1\Figure_S1B\Figure_S1B_2_SDS-PAGE.tif
Figure_S1\Figure_S1B\readme_Fig_S1B.md
Figure_S1\Figure_S1C\Figure_S1C_1_size-exclusion-chromatography.csv
Figure_S1\Figure_S1C\Figure_S1C_2_SDS-PAGE.tif
Figure_S1\Figure_S1C\readme_Fig_S1C.md
Figure_S1\Figure_S1D\Figure_S1D_SEC-SLS.csv
Figure_S1\Figure_S1D\readme_Fig_S1D.md
Figure_S1\Figure_S1E\Figure_S1E_UV-vis.csv
Figure_S1\Figure_S1E\readme_Fig_S1E.md
Figure_S1\Figure_S1F\Figure_S1F_TLC_time_course_0_raw.Tif
Figure_S1\Figure_S1F\Figure_S1F_TLC_time_course_1_background-corrected.tif
Figure_S1\Figure_S1F\readme_Fig_S1F.md
Figure_S1\Figure_S1G\Figure_S1G_1_SDS-PAGE_cGAS.tif
Figure_S1\Figure_S1G\Figure_S1G_2_SDS-PAGE_mGFP-cGAS.tif
Figure_S1\Figure_S1G\Figure_S1G_3_SDS-PAGE_cGAS-HALO646.tif
Figure_S1\Figure_S1G\Figure_S1G_4_SDS-PAGE_biotin-Avi-cGAS-CD.tif
Figure_S1\Figure_S1G\readme_Fig_S1G.md
Figure_S2\Figure_S2A_IMAC_Heparin
Figure_S2\Figure_S2B_SEC
Figure_S2\Figure_S2C_SDS-PAGE
Figure_S2\Figure_S2D_PhosTag-PAGE
Figure_S2\Figure_S2E_SEC-SLS
Figure_S2\Figure_S2F_nanoDSF
Figure_S2\Figure_S2G_SDS-PAGE
Figure_S2\Figure_S2H_condensation
Figure_S2\Figure_S2I_MS
Figure_S2\Figure_SJ_TLC
Figure_S2\Fig_S2_preview.png
Figure_S2\readme_Fig_S2.md
Figure_S2\Figure_S2A_IMAC_Heparin\Figure_S2A_0_IMAC.csv
Figure_S2\Figure_S2A_IMAC_Heparin\Figure_S2A_1_Heparin.csv
Figure_S2\Figure_S2A_IMAC_Heparin\readme_Fig_S2A.md
Figure_S2\Figure_S2B_SEC\Figure_S2B_SEC_1_pN.csv
Figure_S2\Figure_S2B_SEC\Figure_S2B_SEC_2_N.csv
Figure_S2\Figure_S2B_SEC\readme_Fig_S2B.md
Figure_S2\Figure_S2C_SDS-PAGE\Figure_S2C_SDS-PAGE_N_pN.tif
Figure_S2\Figure_S2C_SDS-PAGE\readme_Fig_S2C.md
Figure_S2\Figure_S2D_PhosTag-PAGE\Figure_S2D_PhosTag_N_pN.tif
Figure_S2\Figure_S2D_PhosTag-PAGE\readme_Fig_S2D.md
Figure_S2\Figure_S2E_SEC-SLS\Figure_S2E_SEC_SLS_N_pN.csv
Figure_S2\Figure_S2E_SEC-SLS\readme_Fig_S2E.md
Figure_S2\Figure_S2F_nanoDSF\Figure_S2F_nanoDSF.csv
Figure_S2\Figure_S2F_nanoDSF\readme_Fig_S2F.md
Figure_S2\Figure_S2G_SDS-PAGE\Figure_S2G_SDS-PAGE_pN_N_variants.tif
Figure_S2\Figure_S2G_SDS-PAGE\readme_Fig_S2G.md
Figure_S2\Figure_S2H_condensation\confocal_microscopy_images
Figure_S2\Figure_S2H_condensation\Figure_S2H_condensation.csv
Figure_S2\Figure_S2H_condensation\Figure_S2H_fitted_parameters.txt
Figure_S2\Figure_S2H_condensation\readme_Fig_S2H.md
Figure_S2\Figure_S2H_condensation\confocal_microscopy_images\MAX_N_10uM_100mM_NaCl_MgCl2_5mM_1h_fr4.tif
Figure_S2\Figure_S2H_condensation\confocal_microscopy_images\MAX_N_10uM_150mM_NaCl_MgCl2_5mM_1h_fr23.tif
Figure_S2\Figure_S2H_condensation\confocal_microscopy_images\MAX_N_10uM_300mM_NaCl_MgCl2_5mM_1h_fr5.tif
Figure_S2\Figure_S2H_condensation\confocal_microscopy_images\MAX_N_10uM_50mM_NaCl_MgCl2_5mM_1h_ fr4.tif
Figure_S2\Figure_S2H_condensation\confocal_microscopy_images\MAX_N_10uM_75mM_NaCl_MgCl2_5mM_1h_fr14.tif
Figure_S2\Figure_S2H_condensation\confocal_microscopy_images\MAX_pN_10uM_100mM_NaCl_MgCl2_5mM_1h_fr7.tif
Figure_S2\Figure_S2H_condensation\confocal_microscopy_images\MAX_pN_10uM_150mM_NaCl_MgCl2_5mM_1h_fr9.tif
Figure_S2\Figure_S2H_condensation\confocal_microscopy_images\MAX_pN_10uM_300mM_NaCl_MgCl2_5mM_1h_fr4.tif
Figure_S2\Figure_S2H_condensation\confocal_microscopy_images\MAX_pN_10uM_50mM_NaCl_MgCl2_5mM_1h_ fr5.tif
Figure_S2\Figure_S2H_condensation\confocal_microscopy_images\MAX_pN_10uM_75mM_NaCl_MgCl2_5mM_1h_fr18.tif
Figure_S2\Figure_S2I_MS\Figure_S2I_intact_mass_N_denatured.csv
Figure_S2\Figure_S2I_MS\Figure_S2I_intact_mass_pN_native.csv
Figure_S2\Figure_S2I_MS\Figure_S2I_Report_intact_mass_analysis_Spectroswiss.pdf
Figure_S2\Figure_S2I_MS\readme_Fig_S2I.md
Figure_S2\Figure_SJ_TLC\Figure_S2J_0_raw.Tif
Figure_S2\Figure_SJ_TLC\Figure_S2J_1_bg_50_corrected.tif
Figure_S2\Figure_SJ_TLC\readme_Fig_S2J.md
Figure_S3\Figure_S3A
Figure_S3\Figure_S3B
Figure_S3\Fig_S3_preview.png
Figure_S3\readme_Fig_S3.md
Figure_S3\Figure_S3A\Figure_S3A_N_titration.csv
Figure_S3\Figure_S3A\Figure_S3A_N_titration_TLC.tif
Figure_S3\Figure_S3B\Figure_S3B_DNA_titration.csv
Figure_S3\Figure_S3B\Figure_S3B_DNA_titration_TLC.tif
Figure_S4\Figure_S4A_BLI_20-bp-DNA.csv
Figure_S4\Figure_S4B_BLI_45-bp-DNA.csv
Figure_S4\Fig_S4_preview.png
Figure_S4\readme_Fig_S4.md
Figure_S5\Figure_S5A
Figure_S5\Figure_S5B
Figure_S5\Figure_S5C
Figure_S5\Fig_S5_preview.png
Figure_S5\readme_Fig_S5.md
Figure_S5\Figure_S5A\01uM_cGAS_N_10uM.tif
Figure_S5\Figure_S5A\01uM_cGAS_pN_10uM.tif
Figure_S5\Figure_S5A\05uM_cGAS_N_10uM.tif
Figure_S5\Figure_S5A\05uM_cGAS_pN_10uM.tif
Figure_S5\Figure_S5A\09uM_cGAS_N_10uM.tif
Figure_S5\Figure_S5A\09uM_cGAS_pN_10uM.tif
Figure_S5\Figure_S5A\0uM_cGAS_N_10uM.tif
Figure_S5\Figure_S5A\0uM_cGAS_pN_10uM.tif
Figure_S5\Figure_S5A\readme_Fig_S5A.md
Figure_S5\Figure_S5B\Figure_S5B.csv
Figure_S5\Figure_S5B\raw_confocal_microscopy_images
Figure_S5\Figure_S5B\readme_Fig_S5B.md
Figure_S5\Figure_S5B\raw_confocal_microscopy_images\01uM_cGAS_N_10uM_multigrid.tif
Figure_S5\Figure_S5B\raw_confocal_microscopy_images\01uM_cGAS_pN_10uM_multigrid.tif
Figure_S5\Figure_S5B\raw_confocal_microscopy_images\05uM_cGAS_N_10uM_multigrid.tif
Figure_S5\Figure_S5B\raw_confocal_microscopy_images\05uM_cGAS_pN_10uM_multigrid.tif
Figure_S5\Figure_S5B\raw_confocal_microscopy_images\09uM_cGAS_N_10uM_multigrid.tif
Figure_S5\Figure_S5B\raw_confocal_microscopy_images\09uM_cGAS_pN_10uM_multigrid.tif
Figure_S5\Figure_S5B\raw_confocal_microscopy_images\0uM_cGAS_N_10uM_multigrid.tif
Figure_S5\Figure_S5B\raw_confocal_microscopy_images\0uM_cGAS_pN_10uM_multigrid.tif
Figure_S5\Figure_S5C\cGAS_1uM_dsDNA_0nM.tif
Figure_S5\Figure_S5C\cGAS_1uM_dsDNA_1000nM.tif
Figure_S5\Figure_S5C\cGAS_1uM_dsDNA_100nM.tif
Figure_S5\Figure_S5C\cGAS_1uM_dsDNA_2500nM.tif
Figure_S5\Figure_S5C\cGAS_1uM_dsDNA_250nM.tif
Figure_S5\Figure_S5C\cGAS_1uM_dsDNA_500nM.tif
Figure_S5\Figure_S5C\N_10uM_dsDNA_0nM_cGAS_1uM.tif
Figure_S5\Figure_S5C\N_10uM_dsDNA_1000nM_cGAS_1uM.tif
Figure_S5\Figure_S5C\N_10uM_dsDNA_100nM_cGAS_1uM.tif
Figure_S5\Figure_S5C\N_10uM_dsDNA_2500nM_cGAS_1uM.tif
Figure_S5\Figure_S5C\N_10uM_dsDNA_250nM_cGAS_1uM.tif
Figure_S5\Figure_S5C\N_10uM_dsDNA_500nM_cGAS_1uM.tif
Figure_S5\Figure_S5C\pN_10uM_dsDNA_0nM_cGAS_1uM.tif
Figure_S5\Figure_S5C\pN_10uM_dsDNA_1000nM_cGAS_1uM.tif
Figure_S5\Figure_S5C\pN_10uM_dsDNA_100nM_cGAS_1uM.tif
Figure_S5\Figure_S5C\pN_10uM_dsDNA_2500nM_cGAS_1uM.tif
Figure_S5\Figure_S5C\pN_10uM_dsDNA_250nM_cGAS_1uM.tif
Figure_S5\Figure_S5C\pN_10uM_dsDNA_500nM_cGAS_1uM.tif
Figure_S5\Figure_S5C\readme_Fig_S5C.md
Figure_S6\Figure_S6A
Figure_S6\Figure_S6B
Figure_S6\Figure_S6C
Figure_S6\Fig_S6_preview.png
Figure_S6\readme_Fig_S6.md
Figure_S6\Figure_S6A\1_N_250V_100um-[Cy5].tif
Figure_S6\Figure_S6A\2_pN_250V_100um-[Cy5].tif
Figure_S6\Figure_S6A\3_cGAS_250V_100um-[Cy5].tif
Figure_S6\Figure_S6A\4_DNA_250V_100um-[Cy5].tif
Figure_S6\Figure_S6B\1_N_8bit_inverted.tif
Figure_S6\Figure_S6B\2_pN_8bit_inverted.tif
Figure_S6\Figure_S6B\3_cGAS_8bit_inverted.tif
Figure_S6\Figure_S6B\4_DNA_8bit_inverted.tif
Figure_S6\Figure_S6B\Fig_S6B_quantification_free_DNA.csv
Figure_S6\Figure_S6C\Fig_S6C_fraction_free_DNA.csv