.Selection doc FILE: : SELECTREF
[Enter selection document file name containing the numbers of
the input images in the first column.]
.Expected size of the object: 25
[The search for translation parameters will be restricted to
+/- (NSAM-object_size)/2. For expected object size => actual
object size there will be no search for translation parameters.]
.FIRST AND LAST RING: 5,15
[Only rings with radii in the range 5-15 will be analyzed.]
.ENTER TEMPLATE FOR 2-D EXPERIMENTAL PROJECTIONS NAME: DAT***
[Give the template name of the existing file series of 2D data
projections.]
.Selection doc FILE: : SELECT
[Enter selection document file name containing the numbers of
the input images in the first column.]
.DOCUMENT FILE NAME: ANGLES101
[This is the only output produced by this program.
It contains 6 columns:
1. Number of the most similar reference projection.
When this number is larger than the number of
reference projections, the most similar is the
mirrored projection (see note 1).
2. Not-normalized correlation coefficient.
Can be used as a similarity measure.
3. Psi angle.
In-plane rotation angle determined.
4. X-shift.
X translation determined.
5. Y-shift.
Y translation determined.
6. Experimental projection number.]
NOTES
Example of the Spider batch program implementing 3D alignment of projections procedure: ;b11.alq ;rotate and shift images according to the parameters from AP MQ alignment md vb off md set mp 2 ; Number of reference image is 87 x87=87 do lb1 i=1,900 x55=x0 ud ic,x55,x21,x22,x23,x24,x25,x26 docapmq if(x21.gt.x87) goto lb2 rt sq win/ser00x26 ali/sar00x26 x23 x24,x25 goto lb1 lb2 rt sq win/ser00x26 _1 x23 x24,x25 mr _1 ali/sar00x26 Y lb1 en