.SELECTION DOC FILE: IMAGES127
[Enter the name of the document file containing projection
file numbers in the first column.]
.RADIUS OF RESTORED OBJECT: 23
[Enter radius of reconstructed object (smaller than the
volume size). The reconstruction is calculated inside the
sphere only.]
.ANGLES DOC FILE: ANGLES27
[Enter the name of the document file containing Eulerian
angles for the projections used (psi, theta, phi).]
.DOES YOUR VOLUME HAVE SYMMETRIES? (Y/N): Y
[Answer Yes if you want the symmetries to be internally
enforced on your volume. The reconstruction in this case is
calculated as though the projections were multiplied
as many times as there are symmetries.]
.RECONSTRUCTED 3-D FILE: VOLUME1001
[Name of the output file.]
.LAMBDA, CORRECTION LIMIT: 1.0e-4,0.0
[Lambda determines the weight of corrections. Too small a
value results in long time of calculations; too large
value will terminate the iterations too soon.
Correction limit is used to terminate the iterations.
When the squared correction of the structure becomes
smaller than the preset value, the iterations are
terminated. When correction limit is set to zero,
this criterion is not used.]
.ITERATION LIMIT, MODE: 25,8
[Program will terminate after number of iterations given.
Mode determines the constraints used:
1 - frequency limit (smoothing),
2 - min constraint,
5 - max constraint.
Any combination of constraints can be used. The mode answered
should be the sum of any single modes, for example mode=7
activates both min and max constraints, mode=8 all three of
them.]
.MINIMUM, MAXIMUM: 1.77,1.90
[Values of min and max constraints. They relate to the values
in 2D projection data.]
.SMOOTHING CONST (0-0.999):0.9994
[Smoothing constant determines relative weight of the low-pass
filtration. See note #3.]
NOTES
lambda smoothing const res'n limit (pixels) .5E-3 0.990 3 .5E-3 0.997 5 .1E-3 0.9994 10