.OUTPUT FROM AP MD DOCUMENT: APMD101
[Enter the name of the document file produced by the 'AP MD'
command. This document file should contain 3 columns:
1.Number of the most 2.Distance. 3. psi angle similar reference Can be used as In-plane rotation projection. When a similarity measure. angle determined. this number is larger Output from than number of AP MD document. reference projections the most similar is a mirrored projection (see appended batch program).
.DOCUMENT: ANGLES101
[Enter the name for the angular document file that will
be produced. This document file will contain three
columns with the three Eulerian angles psi, theta, and phi.
It can be subsequently used in the 'BP RP' operation to
calculate a refined 3D reconstruction.]
NOTES
The command 'VO MD' is equivalent to the following SPIDER batch program:; Prepare ang doc file after the alignment of projections ; ; ANGLES101 - document file produced by AP MD command ; ang288 - output ang document file to be used for 3D reconstruction ; x77 - number of 2D reference projections whose angles are stored ; in the ang document file 'angsss' in the order (psi, theta, phi) x77=5266 do lb1 i=1,129 ud ic,x0,x51,x52,x41 ANGLES101 x61=x51 if(x51.le.x77) goto lb3 x61=x51-x77 lb3 ud ic,x61,x22,x42,x43 angsss if(x51.le.x77) goto lb4 x41=x41+180.0 x42=180.0-x42 x43=x43+180.0 lb4 sd x0,x41,x42,x43 ang288 lb1 en