For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).
For single cell transcriptome analysis, the protocol for single-cell RNA-Seq has been published previously (Tang et al., 2010; Tang et al., 2009). Briefly, after a MACS or FACS procedure, a single PGC or somatic cell was randomly picked by using a mouth pipette and transferred into lysate buffer. And for DNA methylome analysis, the KIT-positive PGCs that were obtained by FACS were further washed with DPBS, and then DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (Qiagen).