Analysis of transcript expression and core promoter DNA sequences of high lipid content tissues in human and fruit fly

Gene expression plays a fundamental role in defining the characteristics of living organisms. To
deepen our understanding of tissue-specific gene expression, we analyzed transcript variant
enrichment across different tissues in human and Drosophila. Our study focused on three structurally
and functionally distinct, lipid-rich organs: the brain, adipose tissue, and testis. We characterized the
transcript profiles of these tissues and examined evolutionarily conserved pathways between
humans and Drosophila. Additionally, we analyzed the flanking sequences of transcriptional start
sites of tissue-enriched transcripts. Our findings suggest that Drosophila tissues exhibit more distinct
regulation of gene expression, whereas human gene expression is more generalized, likely relying
more heavily on distal regulatory elements for tissue-specific expression. Through network analysis,
we identified shared central pathways among these tissues and highlighted the evolutionary
potential of highly enriched testis-specific transcripts. This work provides valuable insights into the
mechanisms underlying tissue-specific gene expression and evolutionary conservation.