{
  "access": {
    "embargo": {
      "active": false,
      "reason": null
    },
    "files": "public",
    "record": "public",
    "status": "open"
  },
  "created": "2024-12-09T06:19:56.321576+00:00",
  "custom_fields": {},
  "deletion_status": {
    "is_deleted": false,
    "status": "P"
  },
  "files": {
    "count": 1,
    "enabled": true,
    "entries": {
      "fulltext.pdf": {
        "access": {
          "hidden": false
        },
        "checksum": "md5:97e3ed3fba6010a921ead0f7a39cd31e",
        "ext": "pdf",
        "id": "05b779bb-cfcd-46de-8a15-a3a1b4a8c0ba",
        "key": "fulltext.pdf",
        "links": {
          "content": "https://zenodo.org/api/records/14325907/files/fulltext.pdf/content",
          "iiif_api": "https://zenodo.org/api/iiif/record:14325907:fulltext.pdf/full/full/0/default.png",
          "iiif_base": "https://zenodo.org/api/iiif/record:14325907:fulltext.pdf",
          "iiif_canvas": "https://zenodo.org/api/iiif/record:14325907/canvas/fulltext.pdf",
          "iiif_info": "https://zenodo.org/api/iiif/record:14325907:fulltext.pdf/info.json",
          "self": "https://zenodo.org/api/records/14325907/files/fulltext.pdf"
        },
        "metadata": null,
        "mimetype": "application/pdf",
        "size": 258307,
        "storage_class": "L"
      }
    },
    "order": [],
    "total_bytes": 258307
  },
  "id": "14325907",
  "is_draft": false,
  "is_published": true,
  "links": {
    "access": "https://zenodo.org/api/records/14325907/access",
    "access_grants": "https://zenodo.org/api/records/14325907/access/grants",
    "access_links": "https://zenodo.org/api/records/14325907/access/links",
    "access_request": "https://zenodo.org/api/records/14325907/access/request",
    "access_users": "https://zenodo.org/api/records/14325907/access/users",
    "archive": "https://zenodo.org/api/records/14325907/files-archive",
    "archive_media": "https://zenodo.org/api/records/14325907/media-files-archive",
    "communities": "https://zenodo.org/api/records/14325907/communities",
    "communities-suggestions": "https://zenodo.org/api/records/14325907/communities-suggestions",
    "doi": "https://doi.org/10.1182/blood.v98.7.2220",
    "draft": "https://zenodo.org/api/records/14325907/draft",
    "file_modification": "https://zenodo.org/api/records/14325907/file-modification",
    "files": "https://zenodo.org/api/records/14325907/files",
    "latest": "https://zenodo.org/api/records/14325907/versions/latest",
    "latest_html": "https://zenodo.org/records/14325907/latest",
    "media_files": "https://zenodo.org/api/records/14325907/media-files",
    "parent": "https://zenodo.org/api/records/14325906",
    "parent_html": "https://zenodo.org/records/14325906",
    "preview_html": "https://zenodo.org/records/14325907?preview=1",
    "quota_increase": "https://zenodo.org/api/records/14325907/quota-increase",
    "request_deletion": "https://zenodo.org/api/records/14325907/request-deletion",
    "requests": "https://zenodo.org/api/records/14325907/requests",
    "reserve_doi": "https://zenodo.org/api/records/14325907/draft/pids/doi",
    "self": "https://zenodo.org/api/records/14325907",
    "self_doi": "https://doi.org/10.1182/blood.v98.7.2220",
    "self_doi_html": "https://zenodo.org/doi/10.1182/blood.v98.7.2220",
    "self_html": "https://zenodo.org/records/14325907",
    "self_iiif_manifest": "https://zenodo.org/api/iiif/record:14325907/manifest",
    "self_iiif_sequence": "https://zenodo.org/api/iiif/record:14325907/sequence/default",
    "thumbnails": {
      "10": "https://zenodo.org/api/iiif/record:14325907:fulltext.pdf/full/%5E10,/0/default.jpg",
      "100": "https://zenodo.org/api/iiif/record:14325907:fulltext.pdf/full/%5E100,/0/default.jpg",
      "1200": "https://zenodo.org/api/iiif/record:14325907:fulltext.pdf/full/%5E1200,/0/default.jpg",
      "250": "https://zenodo.org/api/iiif/record:14325907:fulltext.pdf/full/%5E250,/0/default.jpg",
      "50": "https://zenodo.org/api/iiif/record:14325907:fulltext.pdf/full/%5E50,/0/default.jpg",
      "750": "https://zenodo.org/api/iiif/record:14325907:fulltext.pdf/full/%5E750,/0/default.jpg"
    },
    "versions": "https://zenodo.org/api/records/14325907/versions"
  },
  "media_files": {
    "count": 1,
    "enabled": true,
    "entries": {
      "fulltext.pdf.ptif": {
        "access": {
          "hidden": true
        },
        "ext": "ptif",
        "id": "a9c5ca14-1018-4928-8f79-535514b093c7",
        "key": "fulltext.pdf.ptif",
        "links": {
          "content": "https://zenodo.org/api/records/14325907/files/fulltext.pdf.ptif/content",
          "self": "https://zenodo.org/api/records/14325907/files/fulltext.pdf.ptif"
        },
        "metadata": null,
        "mimetype": "application/octet-stream",
        "processor": {
          "source_file_id": "05b779bb-cfcd-46de-8a15-a3a1b4a8c0ba",
          "status": "finished",
          "type": "image-tiles"
        },
        "size": 0,
        "storage_class": "L"
      }
    },
    "order": [],
    "total_bytes": 0
  },
  "metadata": {
    "creators": [
      {
        "affiliations": [
          {
            "name": "From the Department of Morphology and Embryology, Human Anatomy Section, University of Ferrara, Ferrara, Italy; Department of Biomorphology, \"G. D'Annunzio\" University of Chieti, Chieti Scalo, Italy; Institute of Histology and General Embryology, University of Bologna, Bologna, Italy; \"L.A. Seragnoli\" Institute of Hematology and Clinical Oncology, University of Bologna, Bologna, Italy; and Department of Human Normal Morphology, University of Trieste, Trieste, Italy."
          }
        ],
        "person_or_org": {
          "family_name": "Secchiero",
          "given_name": "Paola",
          "name": "Secchiero, Paola",
          "type": "personal"
        }
      },
      {
        "affiliations": [
          {
            "name": "From the Department of Morphology and Embryology, Human Anatomy Section, University of Ferrara, Ferrara, Italy; Department of Biomorphology, \"G. D'Annunzio\" University of Chieti, Chieti Scalo, Italy; Institute of Histology and General Embryology, University of Bologna, Bologna, Italy; \"L.A. Seragnoli\" Institute of Hematology and Clinical Oncology, University of Bologna, Bologna, Italy; and Department of Human Normal Morphology, University of Trieste, Trieste, Italy."
          }
        ],
        "person_or_org": {
          "family_name": "Gonelli",
          "given_name": "Arianna",
          "name": "Gonelli, Arianna",
          "type": "personal"
        }
      },
      {
        "affiliations": [
          {
            "name": "From the Department of Morphology and Embryology, Human Anatomy Section, University of Ferrara, Ferrara, Italy; Department of Biomorphology, \"G. D'Annunzio\" University of Chieti, Chieti Scalo, Italy; Institute of Histology and General Embryology, University of Bologna, Bologna, Italy; \"L.A. Seragnoli\" Institute of Hematology and Clinical Oncology, University of Bologna, Bologna, Italy; and Department of Human Normal Morphology, University of Trieste, Trieste, Italy."
          }
        ],
        "person_or_org": {
          "family_name": "Celeghini",
          "given_name": "Claudio",
          "name": "Celeghini, Claudio",
          "type": "personal"
        }
      },
      {
        "affiliations": [
          {
            "name": "From the Department of Morphology and Embryology, Human Anatomy Section, University of Ferrara, Ferrara, Italy; Department of Biomorphology, \"G. D'Annunzio\" University of Chieti, Chieti Scalo, Italy; Institute of Histology and General Embryology, University of Bologna, Bologna, Italy; \"L.A. Seragnoli\" Institute of Hematology and Clinical Oncology, University of Bologna, Bologna, Italy; and Department of Human Normal Morphology, University of Trieste, Trieste, Italy."
          }
        ],
        "person_or_org": {
          "family_name": "Mirandola",
          "given_name": "Prisco",
          "name": "Mirandola, Prisco",
          "type": "personal"
        }
      },
      {
        "affiliations": [
          {
            "name": "From the Department of Morphology and Embryology, Human Anatomy Section, University of Ferrara, Ferrara, Italy; Department of Biomorphology, \"G. D'Annunzio\" University of Chieti, Chieti Scalo, Italy; Institute of Histology and General Embryology, University of Bologna, Bologna, Italy; \"L.A. Seragnoli\" Institute of Hematology and Clinical Oncology, University of Bologna, Bologna, Italy; and Department of Human Normal Morphology, University of Trieste, Trieste, Italy."
          }
        ],
        "person_or_org": {
          "family_name": "Guidotti",
          "given_name": "Lia",
          "name": "Guidotti, Lia",
          "type": "personal"
        }
      },
      {
        "affiliations": [
          {
            "name": "From the Department of Morphology and Embryology, Human Anatomy Section, University of Ferrara, Ferrara, Italy; Department of Biomorphology, \"G. D'Annunzio\" University of Chieti, Chieti Scalo, Italy; Institute of Histology and General Embryology, University of Bologna, Bologna, Italy; \"L.A. Seragnoli\" Institute of Hematology and Clinical Oncology, University of Bologna, Bologna, Italy; and Department of Human Normal Morphology, University of Trieste, Trieste, Italy."
          }
        ],
        "person_or_org": {
          "family_name": "Visani",
          "given_name": "Giuseppe",
          "name": "Visani, Giuseppe",
          "type": "personal"
        }
      },
      {
        "affiliations": [
          {
            "name": "From the Department of Morphology and Embryology, Human Anatomy Section, University of Ferrara, Ferrara, Italy; Department of Biomorphology, \"G. D'Annunzio\" University of Chieti, Chieti Scalo, Italy; Institute of Histology and General Embryology, University of Bologna, Bologna, Italy; \"L.A. Seragnoli\" Institute of Hematology and Clinical Oncology, University of Bologna, Bologna, Italy; and Department of Human Normal Morphology, University of Trieste, Trieste, Italy."
          }
        ],
        "person_or_org": {
          "family_name": "Capitani",
          "given_name": "Silvano",
          "name": "Capitani, Silvano",
          "type": "personal"
        }
      },
      {
        "affiliations": [
          {
            "name": "From the Department of Morphology and Embryology, Human Anatomy Section, University of Ferrara, Ferrara, Italy; Department of Biomorphology, \"G. D'Annunzio\" University of Chieti, Chieti Scalo, Italy; Institute of Histology and General Embryology, University of Bologna, Bologna, Italy; \"L.A. Seragnoli\" Institute of Hematology and Clinical Oncology, University of Bologna, Bologna, Italy; and Department of Human Normal Morphology, University of Trieste, Trieste, Italy."
          }
        ],
        "person_or_org": {
          "family_name": "Zauli",
          "given_name": "Giorgio",
          "name": "Zauli, Giorgio",
          "type": "personal"
        }
      }
    ],
    "description": "Tumor necrosis factor (TNF)\u2013related apoptosis-inducing ligand (TRAIL) induced both cytotoxic (apoptosis) and cytostatic (cell cycle perturbation) effects on the human myeloid K562 cell line. TRAIL stimulated caspase 3 and nitric oxide synthase (NOS) activities, and both pathways cooperate in mediating inhibition of K562 survival/growth. This was demonstrated by the ability of z-VAD-fmk, a broad inhibitor of effector caspases, and N-nitro-l-arginine methyl ester (L-NAME), an NOS pharmacologic inhibitor, to completely (z-VAD-fmk) or partially (L-NAME) suppress the TRAIL-mediated inhibitory activity. Moreover, z-VAD-fmk was able to block TRAIL-mediated apoptosis and cell cycle abnormalities and increase of NOS activity. The addition of the NO donor sodium nitroprusside (SNP) to K562 cells reproduced the cytostatic effect of TRAIL without inducing apoptosis. When TRAIL was associated to SNP, a synergistic increase of apoptosis and inhibition of clonogenic activity was observed in K562 cells as well as in other myeloblastic (HEL, HL-60), lymphoblastic (Jurkat, SupT1), and multiple myeloma (RPMI 8226) cell lines. Although SNP greatly augmented TRAIL-mediated antileukemic activity also on primary leukemic blasts, normal erythroid and granulocytic cells were less sensitive to the cytotoxicity mediated by TRAIL with or without SNP. These data indicate that TRAIL promotes cytotoxicity in leukemic cells by activating effector caspases, which directly lead to apoptosis and stimulate NO production, which mediates cell cycle abnormalities. Both mechanisms seem to be essential for TRAIL-mediated cytotoxicity.",
    "languages": [
      {
        "id": "eng",
        "title": {
          "en": "English"
        }
      }
    ],
    "publication_date": "2002-07-27",
    "publisher": "Zenodo",
    "resource_type": {
      "id": "publication-article",
      "title": {
        "de": "Zeitschriftenartikel",
        "en": "Journal article"
      }
    },
    "rights": [
      {
        "description": {
          "en": ""
        },
        "id": "other-open",
        "title": {
          "en": "Other (Open)"
        }
      }
    ],
    "title": "Activation of the nitric oxide synthase pathway represents a key component of tumor necrosis factor\u2013related apoptosis-inducing ligand\u2013mediated cytotoxicity on hematologic malignancies"
  },
  "parent": {
    "access": {
      "owned_by": {
        "user": "1231459"
      },
      "settings": {
        "accept_conditions_text": null,
        "allow_guest_requests": false,
        "allow_user_requests": false,
        "secret_link_expiration": 0
      }
    },
    "communities": {
      "default": "1cbc1580-a34f-40cd-b001-c0cbc1b1083c",
      "entries": [
        {
          "access": {
            "member_policy": "open",
            "members_visibility": "public",
            "record_submission_policy": "open",
            "review_policy": "members",
            "visibility": "public"
          },
          "children": {
            "allow": false
          },
          "created": "2024-12-07T16:00:25.151710+00:00",
          "custom_fields": {},
          "deletion_status": {
            "is_deleted": false,
            "status": "P"
          },
          "id": "1cbc1580-a34f-40cd-b001-c0cbc1b1083c",
          "links": {},
          "metadata": {
            "description": "Mirror of bronze OA or gold OA papers connected to Italy.",
            "title": "Italy papers mirror",
            "type": {
              "id": "topic"
            }
          },
          "revision_id": 4,
          "slug": "it-mirror",
          "updated": "2024-12-07T16:01:13.249552+00:00"
        }
      ],
      "ids": [
        "1cbc1580-a34f-40cd-b001-c0cbc1b1083c"
      ]
    },
    "id": "14325906",
    "pids": {}
  },
  "pids": {
    "doi": {
      "identifier": "10.1182/blood.v98.7.2220",
      "provider": "external"
    },
    "oai": {
      "identifier": "oai:zenodo.org:14325907",
      "provider": "oai"
    }
  },
  "revision_id": 4,
  "stats": {
    "all_versions": {
      "data_volume": 35904673.0,
      "downloads": 139,
      "unique_downloads": 134,
      "unique_views": 7,
      "views": 7
    },
    "this_version": {
      "data_volume": 35904673.0,
      "downloads": 139,
      "unique_downloads": 134,
      "unique_views": 7,
      "views": 7
    }
  },
  "status": "published",
  "swh": {},
  "updated": "2024-12-09T06:19:56.815827+00:00",
  "versions": {
    "index": 1,
    "is_latest": true
  }
}