Funding provided by: Deutsche Forschungsgemeinschaft
ROR ID: https://ror.org/018mejw64
Award Number:
scRNA-seq of blood samples from Buteo buteo nestlings infected with Leucocytozoon toddi
", "type": { "id": "methods", "title": { "de": "Methoden", "en": "Methods" } } } ], "creators": [ { "affiliations": [ { "name": "Bielefeld University" } ], "person_or_org": { "family_name": "Rapp", "given_name": "Tim Maximilian", "identifiers": [ { "identifier": "0009-0001-5623-4389", "scheme": "orcid" } ], "name": "Rapp, Tim Maximilian", "type": "personal" } }, { "affiliations": [ { "name": "Heinrich Heine University D\u00fcsseldorf" } ], "person_or_org": { "family_name": "Lautwein", "given_name": "Tobias", "name": "Lautwein, Tobias", "type": "personal" } }, { "affiliations": [ { "name": "Bielefeld University" } ], "person_or_org": { "family_name": "Chakarov", "given_name": "Nayden", "name": "Chakarov, Nayden", "type": "personal" } } ], "description": "Parasites and hosts perform fine-tuned cellular and molecular interactions, known from few well-explored model systems. Recent methodological advances allow such deep insights into non-model and phylogenetically remote organisms and their intense reciprocal manipulations within symbioses. We applied single cell RNA-sequencing to blood samples of long-lived wild avian hosts, infected at high and low intensity with malaria-related parasites. We identify diverse parasitic tools for immune evasion and potential manipulation of the avian host immune response. The transcriptomic response of immune cells was decoupled from the infection load, consistent with evidently low pathogenicity. Changes of immune cell populations showed low activation of adaptive immunity, such as clonal expansion of lymphoid cells. This absence of antagonism indicates immunosuppression by the parasite, enabling an adaptive match of life-histories and their long-term coevolution. The identified cell-type specific markers provide an important reference and help to understand the evolutionary forces that shape host immunity in birds. Our study illuminates in rare molecular detail a benign host-parasite interaction and annotates a large coevolutionary gap along the gradient from parasitism to commensalism and mutualism.
", "publication_date": "2026-06-05", "publisher": "Zenodo", "related_identifiers": [ { "identifier": "10.5061/dryad.sbcc2frf2", "relation_type": { "id": "issourceof", "title": { "de": "Ist Quelle von", "en": "Is source of" } }, "scheme": "doi" } ], "resource_type": { "id": "software", "title": { "de": "Software", "en": "Software" } }, "rights": [ { "description": { "en": "A short and simple permissive license with conditions only requiring preservation of copyright and license notices. Licensed works, modifications, and larger works may be distributed under different terms and without source code." }, "id": "mit", "props": { "scheme": "spdx", "url": "https://opensource.org/licenses/MIT" }, "title": { "en": "MIT License" } } ], "subjects": [ { "subject": "Host-parasite interaction" }, { "subject": "Avian malaria" }, { "subject": "scRNA-seq" }, { "subject": "transcriptomics" }, { "subject": "immunosuppression" }, { "subject": "Host response" }, { "subject": "Leucocytozoon" }, { "subject": "Plasmodium" } ], "title": "scRNA-seq of Buteo buteo and Leucocytozoon toddi: Analysis with Seurat (R)" }, "parent": { "access": { "owned_by": { "user": "90070" }, "settings": { "accept_conditions_text": null, "allow_guest_requests": false, "allow_user_requests": false, "secret_link_expiration": 0 } }, "communities": { "default": "734b6452-1f40-4db0-b218-08ab1d568b7f", "entries": [ { "access": { "member_policy": "open", "members_visibility": "public", "record_submission_policy": "open", "review_policy": "open", "visibility": "public" }, "children": { "allow": false }, "created": "2020-02-04T14:03:17.992421+00:00", "custom_fields": {}, "deletion_status": { "is_deleted": false, "status": "P" }, "id": "734b6452-1f40-4db0-b218-08ab1d568b7f", "links": {}, "metadata": { "curation_policy": "The Dryad collection in Zenodo contains datasets mirrored from the Dryad Digital Repository. If you want your dataset to appear here, submit it through https://datadryad.org. No other datasets will be accepted.
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