LOCUS       attB_HA-V2R_2xBbvCI_cle 4669 bp ds-DNA     circular     25-NOV-2024
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      complement(367..673)
                     /label="F1 ori"
     CDS             complement(692..760)
                     /label="LacZ alpha"
     misc_feature    828..834
                     /label="BbvCI site 2"
     primer_bind     838..855
                     /label="M13-fwd"
     primer_bind     854..881
                     /label="T7"
     misc_feature    980..1018
                     /label="AttB"
     misc_feature    1042..1047
                     /label="kozak"
     CDS             1048..1077
                     /label="Translation 1048-1077"
                     /translation="MYPYDVPDYA"
     misc_feature    1051..1077
                     /label="HA tag"
     misc_feature    1078..2192
                     /label="V2R_codon_opt_for_IDT"
     CDS             1078..2192
                     /label="Translation 1078-2192"
                     /translation="MLMASTTSAVPGHPSLPSLPSNSSQERPLDTRDPLLARAELALLSIVFVAVALSNGLVLAALARRGRRGHWAPIHVFIGHLCLADLAVALFQVLPQLAWKATDRFRGPDALCRAVKYLQMVGMYASSYMILAMTLDRHRAICRPMLAYRHGSGAHWNRPVLVAWAFSLLLSLPQLFIFAQRNVEGGSGVTDCWACFAEPWGRRTYVTWIALMVFVAPTLGIAACQVLIFREIHASLVPGPSERPGGRRRGRRTGSPGEGAHVSAAVAKTVRMTLVIVVVYVLCWAPFFLVQLWAAWDPEAPLEGAPFVLLMLLASLNSCTNPWIYASFSSSVSSELRSLLCCARGRTPPSLGPQDESCTTASSSLAKDTSS"
     misc_feature    1141..1149
                     /label="N glycosylation site (NxS/T)"
     misc_feature    1483..1491
                     /label="DRY/H"
     misc_feature    2038..2052
                     /label="NPxxY"
     CDS             2191..2193
                     /translation="*"
     CDS             2193..2193
                     /label="Translation 2193-2193"
                     /translation=""
     misc_feature    complement(2193..2193)
                     /label="double stop"
     misc_feature    2219..2224
                     /label="EcoRI"
     misc_feature    2231..2462
                     /label="bGH poly(A) signal"
     misc_feature    2487..2493
                     /label="BbvCI site1"
     misc_feature    2583..2704
                     /label="SV40 polyA"
     promoter        complement(2713..2731)
                     /label="T3 promoter"
     primer_bind     complement(2748..2768)
                     /label="M13-rev"
     misc_binding    complement(2774..2796)
                     /label="LacO"
     promoter        complement(2800..2830)
                     /label="lac promoter"
     rep_origin      complement(3136..3818)
                     /label="ColE1 origin"
     CDS             complement(3916..4575)
                     /label="AmpR"
ORIGIN
        1 TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC
       61 GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA
      121 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT
      181 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTAAATTG
      241 TAAGCGTTAA TATTTTGTTA AAATTCGCGT TAAATTTTTG TTAAATCAGC TCATTTTTTA
      301 ACCAATAGGC CGAAATCGGC AAAATCCCTT ATAAATCAAA AGAATAGACC GAGATAGGGT
      361 TGAGTGTTGT TCCAGTTTGG AACAAGAGTC CACTATTAAA GAACGTGGAC TCCAACGTCA
      421 AAGGGCGAAA AACCGTCTAT CAGGGCGATG GCCCACTACG TGAACCATCA CCCTAATCAA
      481 GTTTTTTGGG GTCGAGGTGC CGTAAAGCAC TAAATCGGAA CCCTAAAGGG AGCCCCCGAT
      541 TTAGAGCTTG ACGGGGAAAG CCGGCGAACG TGGCGAGAAA GGAAGGGAAG AAAGCGAAAG
      601 GAGCGGGCGC TAGGGCGCTG GCAAGTGTAG CGGTCACGCT GCGCGTAACC ACCACACCCG
      661 CCGCGCTTAA TGCGCCGCTA CAGGGCGCGT CCCATTCGCC ATTCAGGCTG CGCAACTGTT
      721 GGGAAGGGCG ATCGGTGCGG GCCTCTTCGC TATTACGCCA GCTGGCGAAA GGGGGATGTG
      781 CTGCAAGGCG ATTAAGTTGG GTAACGCCAG GGTTTTCCCA GTCACGACCT CAGCCGTTGT
      841 AAAACGACGG CCAGTGAATT GTAATACGAC TCACTATAGG GCGAATTGGG TACCGGGCCN
      901 NNNNATNNNN NATNNNNNAT NNNNNCCCCC TCGAGGTCGA CGATGTAGGT CACGGCATTC
      961 CGGACTCAGC GCTCGAGCCG GCTTGTCGAC GACGGCGGTC TCCGTCGTCA GGATCATCCG
     1021 CAGATTATAC CGCAACTACA CGCCACCATG tatccgtacg acgtaccaga ctacgcaATG
     1081 CTCATGGCGT CCACCACTTC CGCTGTTCCT GGACATCCAT CATTGCCTTC ATTGCCTTCT
     1141 AATTCTTCTC AGGAGAGGCC ACTGGACACC CGGGACCCGC TGCTAGCCCG GGCGGAGCTG
     1201 GCGCTGCTCT CCATAGTCTT TGTGGCTGTG GCCCTGAGCA ATGGCCTGGT GCTGGCGGCC
     1261 TTGGCTAGAA GAGGAAGAAG AGGACATTGG GCACCTATAC ATGTTTTCAT TGGACACTTG
     1321 TGTTTGGCTG ATTTGGCCGT GGCTCTGTTC CAAGTGCTGC CCCAGCTGGC CTGGAAGGCC
     1381 ACCGACCGCT TTAGAGGACC AGATGCTTTG TGTAGAGCTG TTAAATATTT GCAAATGGTT
     1441 GGCATGTATG CCTCCTCCTA CATGATCCTG GCCATGACGC TGGACCGCCA CCGTGCCATC
     1501 TGCCGTCCCA TGCTGGCGTA CCGCCATGGA AGTGGGGCTC ACTGGAACCG GCCGGTGCTA
     1561 GTGGCTTGGG CCTTCTCGCT CCTTCTCAGC CTGCCCCAGC TCTTCATCTT CGCCCAGCGC
     1621 AACGTGGAAG GTGGCAGCGG GGTCACTGAC TGCTGGGCCT GCTTTGCGGA GCCCTGGGGC
     1681 CGTCGCACCT ATGTCACCTG GATTGCCCTG ATGGTGTTCG TGGCACCTAC CCTGGGTATC
     1741 GCCGCCTGCC AGGTGCTCAT CTTCCGGGAG ATTCATGCTA GTTTGGTTCC AGGACCTTCA
     1801 GAAAGACCTG GAGGAAGAAG GAGAGGAAGA AGAACAGGAT CTCCCGGTGA GGGAGCCCAC
     1861 GTGTCAGCAG CTGTGGCCAA GACTGTGAGG ATGACGCTAG TGATTGTGGT CGTCTATGTG
     1921 CTGTGCTGGG CACCCTTCTT CCTGGTGCAG CTGTGGGCCG CGTGGGACCC GGAGGCACCT
     1981 CTGGAAGGGG CGCCCTTTGT GCTACTCATG TTGCTGGCCA GCCTCAACAG CTGCACCAAC
     2041 CCCTGGATCT ATGCATCTTT CAGCAGCAGC GTGTCCTCAG AGCTGCGAAG CTTGCTCTGC
     2101 TGTGCCCGGG GACGCACCCC ACCCAGCCTG GGTCCCCAAG ATGAGTCCTG CACCACCGCC
     2161 AGCTCCTCCC TGGCCAAGGA CACTTCATCG TGAGAGCTGG CCGAGAAGCT GGCCGGCCGA
     2221 ATTCctcgag CTAGAGCTCG CTGATCAGCC TCGACTGTGC CTTCTAGTTG CCAGCCATCT
     2281 GTTGTTTGCC CCTCCCCCGT GCCTTCCTTG ACCCTGGAAG GTGCCACTCC CACTGTCCTT
     2341 TCCTAATAAA ATGAGGAAAT TGCATCGCAT TGTCTGAGTA GGTGTCATTC TATTCTGGGG
     2401 GGTGGGGTGG GGCAGGACAG CAAGGGGGAG GATTGGGAAG ACAATAGCAG GCATGCTGGG
     2461 GAGGATCCTG ATCATAATCA GCCATACCTC AGCCCACATT TGTAGAGGTT TTACTTGCTT
     2521 TAAAAAACCT CCCACACCTC CCCCTGAACC TGAAACATAA AATGAATGCA ATTGTTGTTG
     2581 TTAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA
     2641 CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT
     2701 CTTAGGAGAT CCCCTTTAGT GAGGGTTAAT TTCGAGCTTG GCGTAATCAT GGTCATAGCT
     2761 GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT
     2821 AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC
     2881 ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG
     2941 CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT
     3001 GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT
     3061 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC
     3121 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA
     3181 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA
     3241 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC
     3301 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG
     3361 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC
     3421 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG
     3481 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT
     3541 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT
     3601 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG
     3661 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC
     3721 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA
     3781 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC
     3841 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC
     3901 TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT
     3961 TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT
     4021 ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT
     4081 ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC
     4141 CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA
     4201 TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG
     4261 TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT
     4321 GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC
     4381 AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT
     4441 AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG
     4501 GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC
     4561 TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC
     4621 GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCT
//