READ ME Breakdown of the uploads: MSSettings refer to the settings and instruments used for proteomic analysis KeyResourcesTable has all the information about materials used for the study Tagless Lyso IP Video Demo is the video method for Tagless LysoIP Metabolomic_Fig4 has the raw data for Fig4 Lysosomalproteins_DatasetS1 has the list of lysosomally annotated proteins used for lysosomal enrichment analysis Mitochondrialproteins_DatasetS2 has the list of mitochondrial annotated proteins used for mitochondrial enrichment analysis FLowcytometry_SFig3CD has the raw data for SFig3DC in .fcs format Immunofluorescence_SFig3_HA647_T192.569_LAMP1.4.88, Immunofluoresence_SFig3_HA.647_T192.594_ATPB.488.lsm are immunofluorescence data for SFig3A. HA antibody used at 647nm, TMEM192 at 594nm and either LAMP1 or ATPB at 488nm wavelengt Flowcytometry_SFig3CD_rawdata and Flowcytometry_Fig3BC_rawdata.zip are raw data for SFig3CD and Fig3BC in .fcs format Immunoblot_SFig1C_WCL_panel.tif, Immunoblot_SFig1B.tif, Immunoblot_SFig1C_IP_panel.tif are raw data for SFig1 GCaseactivity_Fig3E.xlsx has raw data for Fig3D and 3E Immunoblot_SFig6_800nm_firstread.tif is first scan of SFig6 immunoblot using primary antibodies described and wavelenght 800nm Immunoblot_SFig6_680nm_secondread.tif is second scan of SFig6 immunoblot using primary antibodies described and wavelenght 680nm Immunoblot_SFIg6_800nm_thirdread.tif is third scan of SFig6 immunoblot using primary antibodies described and wavelenght 800nm Quantifications is quantifications for Fig 2 and 4, SFig 2, 3, 4, 5, 6 iPSC Characterization is the QC table for used iPSCs.