Published August 12, 2024
| Version v2
Dataset
Open
Primary MS data and secondary data for: "Post-proline cleaving enzymes also show specificity to reduced cysteine"
Creators
- 1. Department of Biochemistry, Faculty of Science, Charles University
- 2. AffiPro s.r.o.
- 3. Institute of Microbiology of the Czech Academy of Sciences, BioCeV
-
4.
Czech Academy of Sciences, Institute of Microbiology
Description
raw LC-MS/MS data (Bruker Daltonics - timsTOF Pro and tims TOF SCP) and ESI-MS data (Bruker Daltonics - 15T ESI/MALDI FT-ICR MS) and related MASCOT generic files (.mgf) and search results (.csv) plus corresponding custom databases (*.fasta). Protein identifications were performed using MASCOT or PEAKS programs.
Data were used to:
- extract cleavage preferences of Clarity Ferm AnPEP, ProAlanase or Neprosin. Target samples were: protein mixture (BSA, cytC, bCA2, myoglobin, 14-3-3) or human serum from a healthy donor or HEK cell lysate or human insulin or oxidized bovine insulin beta chain
- profile protein content of Clarity Ferm AnPEP and quantify the proteins
Additional data are under Zenodo entry 10.5281/zenodo.13985598
Linked to the publication:
Postproline Cleaving Enzymes also Show Specificity to Reduced Cysteine.
Kalaninová Z, Portašiková JM, Jirečková B, Polák M, Nováková J, Kavan D, Novák P, Man P.
Anal. Chem. 2024, 96, 48, 19084–19092
doi: 10.1021/acs.analchem.4c04277.
Files
AnPEP-csv.zip
Files
(46.7 GB)
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Additional details
Related works
- Is supplemented by
- Dataset: 10.5281/zenodo.13985598 (DOI)