Climate and nutrition drive gut microbiome variation in a fruit-specialist primate.

Nina Beeby (ninabeeby@gmail.com) and Timothy H Webster (timothy.h.webster@utah.edu), corresponding authors

"Vvariegata_nutrition_16s_reads.tar.gz" is a tarball of fastqs containing all of the raw sequencing reads used in this study.
The prefix of each file name corresponds with the SampleID column in "Beeby_etal_Nutritioni_GM_Dataset.csv"

"Beeby_etal_Nutrition_GM_Dataset.csv" is the summary data file for analyses, containing the following columns:
Date: dd/mm/yyyy format
Year: numerical year
monthnum: numerical months
month name: full month names
individualID: focal animals included in study
age: age-class of focal animals
sex: sex of focal individuals 
SampleID: fecal sample identifier
barcode: sample identifier
batch: Illumina sequencing batch number
temp_30_day: rolling 30 day mean of average temperature
rainfall_30_day: rolling 30 day mean of total rainfall
intake_ndf_30_day: rolling 30 day mean of neutral detergent fiber intake for focal individual 
intake_tnc_30_day: rolling 30 day mean of total non-structural carbohydrate intake for focal individual
intake_ap_30_day: rolling 30 day mean of available protein intake for focal individual
g_total_food_30_day: rolling 30 day mean of total grams of food eaten for focal individual
prop_fruit_30_day: rolling 30 day mean of proportion of fruit in the diet for focal individual
diet_div_30_day: rolling 30 day mean of Shannon'92s dietary diversity for focal individual
ASV_ID: identifier for individual ASVs
count: number of reads for ASV
Confidence: confidence level for taxonomy assignment for ASV
Taxon: full taxonomic classification for ASV
Domain: domain for ASV
Phylum: phylum for ASV
Class: class for ASV
Order: order for ASV
Family: family for ASV
Genus: genus for ASV
Species: species for ASV
Count_ASV: total count for ASV of interest
BactRank: 99% confidence 
rain_discrete: rainfall variable in discretised form (high vs low)
ndf_discrete: neutral detergent fiber variable in discretised form (high vs low)
tnc_discrete: total non-structural carbohydrate variable in discretised form (high vs low)
fat_discrete: fat variable in discretised form (high vs low)
fruit_discrete: proportion of frugivory variable in discretised form (high vs low)
dietdiv_discrete: dietary diversity variable in discretised form (high vs low)