Published September 2, 2013 | Version 2
Software Open

miRprimer

Creators

  • 1. Roskilde Universitet

Description

miRprimer designs primers for PCR amplification of microRNAs as described (Busk (2014). A tool for design of primers for microRNA-specific quantitative RT-qPCR. BMC Bioinformatics. 15, 29) for use with the method miR-specific RT-qPCR (Cirera, S., and Busk, P.K. (2014). Quantification of miRNAs by a simple and specific qPCR method. Methods in Molecular Biology. 1182, 73-81.).
The program was written in Ruby and is available as source code for developers and as an .exe file for easy use.

Notes

Originally published as https://sourceforge.net/projects/mirprimer/

Files

input_miRs.txt

Files (3.3 MB)

Name Size Download all
md5:de07c56c8b6184a6409cd3e271d456c3
445 Bytes Preview Download
md5:033e2618b9f011563ce1d806d92dbd89
3.3 MB Download
md5:8199bfeacee34b5ce2114979d9df905f
17.8 kB Download
md5:31404fe8029f020bd04e1875cb466f53
4.7 kB Preview Download

Additional details

Identifiers

URL
https://sourceforge.net/projects/mirprimer/

References

  • Busk, PK. (2014). A tool for design of primers for microRNA-specific quantitative RT-qPCR. BMC Bioinformatics 15(1):29
  • Cirera, S., and Busk, P.K. (2014). Quantification of miRNAs by a simple and specific qPCR method. Methods in Molecular Biology. 1182, 73-81.
  • Balcells I. Cirera S. and Busk P.K. (2011) Specific and sensitive quantitative RT-PCR of miRNAs with DNA primers. BMC Biotechnol. 11(1), 70.