FIGURE 2. Phylogenetic tree of Maximum Likelihood analyses of Colletotrichum gloeosporioides species complex and its closely related species (including sequences of our strain and a selection of related Colletotrichum spp.,), restricted according to the phylogenetic study of Supplementary Material (Figure S1). Colletotrichum hippeastri (CBS 125376) and C. boninense CBS 123755 were used as outgroup taxa. The tree was built using concatenated sequences of ITS, ACT, CHS-1, GADPH, and TUB2 datasets and the tree topology of the RAxML and Bayesian analyses are similar. The combined gene analysis included 86 strains with 2152 characters after aligned, including gaps (ITS: 593bp, ACT: 282bp, CHS-1: 299bp, GADPH: 277bp, and TUB2: 701bp). The RAxML analysis of the combined dataset yielded the best scoring tree with a final ML optimization likelihood value of -15857.131621. The matrix had 1198 distinct alignment patterns, with 23.12% undetermined characters or gaps. Estimated base frequencies were as follows: A = 0.229650, C = 0.299082, G = 0.241413, T = 0.229855; substitution rates AC = 1.009331, AG = 2.892663, AT = 1.227390, CG = 0.965860, CT = 4.527049, GT = 1.000000; gamma distribution shape parameter α = 0.449663. Bootstrap support values for RAxML equal to or greater than 70% and Bayesian posterior probabilities equal to or greater than 0.90 are given at each node. The new isolate is shown in red and bold.