*****----------------Description of the open data provided in the frame of manuscript  "Plasma-Activated Polydimethylsiloxane Microstructured Pattern with Collagen for Improved Myoblast Cell Guidance"-----------------------*****
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Authors: 	Nikola Slepičková Kasálková 1, Veronika Juřicová 1, Dominik Fajstavr 1, Bára Frýdlová 1, Silvie Rimpelová 2, Václav Švorčík 1, Petr Slepička 1
Afilitaion: 1 	Department of Solid State Engineering, The University of Chemistry and Technology Prague, 166 28 Prague, The Czech Republic;	
	    2 	Department of Biochemistry and Microbiology, The University of Chemistry and Technology Prague, 166 28 Prague, The Czech Republic; 

Contact informations: Nikola Slepičková Kasálková: nikola.kasalkova@vscht.cz

Data manager or custodian: Petr Slepička (petr.slepicka@vscht.cz)

Data were collected: 02/2023-02/2024

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Relevant abrreviations:

- PDMS = polydimethylsiloxane 
- TCPS = tissue culture polystyrene 
- PBS = phosphate buffer
- DAPI = (4´,6-diamidino-2-phenylindole)
- CC = collagen coating

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File name structure:

The following repository contains .zip folders, which are distinguished according to the chosen type of analysis.

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-   Analysis 1	(SEM)
		- A sub-folder labeled "PDMS_negative replicas_pattern size 30 x30 micrometers" containing scans of surface samples in tiff named as: Scan size 100 x 100 micrometers_coated_lyophilized, Scan size 1000 x 1000, 250 x 250 and 100 x 100 micrometers, Scan size 250 x 250 micrometers.
	        - A sub-folder labeled "PDMS_negative replicas_pattern size 50 x50 micrometers" containing scans of surface samples in tiff named as: Scan size 100 x 100 micrometers_coated_lyophilized, Scan size 500 x 500 micrometers.
	
	 - Data description of: Methods used for acquiring/creation of data: scanning electron microscope (FIB-SEM LYRA3 GMU, TESCAN, the accelerating voltage 10 kV) 
				Summary of variables: WD = Working distance; DE = Detector; HV = High Voltage; MAG = Magnification

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-   Analysis 2	(EDS)
		- Data are in a table in a .docx file named: Chemical.composition.of.PDMS.surfaces
			
        - Data description of:	Methods used for acquiring/creation of data: energy-dispersive X-ray spectroscopy (EDS, analyzer X-ManN, 20 mm2 SDD detector, the accelerating voltage 10 kV)
				
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-   Analysis 3	(XPS)
		- A sub-folder labeled "Deconvoluted C1s XPS spectra_PDMS_ negative replicas" containing data in tiff named as: Pattern size 30 × 30 micrometers_after collagen coating, Pattern size 30 × 30 micrometers_before lyophilization, Pattern size 50 × 50 micrometers_after collagen coating, Pattern size 50 × 50 micrometers_before lyophilization.
		- A sub-folder labeled "XPS spectra_PDMS_ negative replicas" containing data in tiff named as: Pattern size 30 × 30 micrometers_after collagen coating, Pattern size 30 × 30 micrometers_before lyophilization, Pattern size 50 × 50 micrometers_after collagen coating, Pattern size 50 × 50 micrometers_before lyophilization.

	- Data description of: Methods used for acquiring/creation of data: X-ray photoelectron spectroscopy (ESCAProbeP Omicron Nanotechnology spectrometer) 
			       Methods used for data processing: CasaXPS software (version 2.3).
			       Description of units: At% = atomic concentration, CPS = counts per second, Pos. = position, FWHM = full width of a peak measured at a half of its maximum height
				     
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-   Analysis 4	(Cytocompatibility Testing)
		- A sub-folder labeled "Cells number" containing data in .xlsx named as: Cells.number.
		- A sub-folder labeled "PDMS_negative replicas_pattern size 30 x 30 micrometers after CC" containing data in tiff named as: Cells_first day, Cells_sixth day, Cells_third day.
		- A sub-folder labeled "PDMS_negative replicas_pattern size 50 x 50 micrometers after CC" containing data in tiff named as: Cells_first day, Cells_sixth day, Cells_third day, Cytoskeleton_third day, Nuclei_third day.
		- A sub-folder labeled "TCPS" containing data in tiff named as: Cells_first day, Cells_sixth day, Cells_third day.
				
	- Data description of:  Methods used for acquiring/creation of data: Fluorescence microscopy (Olympus IX-81, Olympus, EMCCD camera, Japan; xCellence software), staining solution phalloidin-Atto 488 and DAPI in PBS.
				Methods used for data processing: Randomly chosen fields, Number of cells were determined using ImageJ software - counting from photos, averages values and SD (standard deviation) were determined.
				Description of units: scale for "cytoskeleton" and "nuclei" is 50 microns, scale for "cells" is 100 microns