Data File  : C:\VA\2024-04-30-KANG-VA7-069-5 2024-04-30 16-24-55\VA7-069-5-3'-deoxy-ATP.D
Acq. Method: StandardGradientPeptideCC-AERISC18-60dC.M


The Acq. Method's Instrument Parameters for the Run were : 


=====================================================================
                                 MWD
=====================================================================

MWD (G1365D)
============

Peakwidth:            > 0.1 min (2 s response time) (2.5 Hz)
Slit:                                                   4 nm
UV Lamp Required:                                        Yes
Vis Lamp Required:                                        No

Analog Output 1               
Analog 1 Zero Offset:                                    5 %
Analog 1 Attenuation:                               1000 mAU

Analog Output 2               
Analog 2 Zero Offset:                                    5 %
Analog 2 Attenuation:                               1000 mAU

Signals                       
Signal table
   Acquire   Signal   Wavelength   Bandwidth   Use Ref.
                              nm          nm           
-------------------------------------------------------
       Yes Signal A          214           4         No
        No Signal B                                    
        No Signal C                                    
       Yes Signal D          280           4         No
        No Signal E                                    
        No Signal F                                    
        No Signal G                                    
        No Signal H                                    




Prepare Mode                  
Margin for negative Absorbance:                      100 mAU

Autobalance                   
Autobalance Prerun:                                      Yes
Autobalance Postrun:                                      No

Stoptime                      
Stoptime Mode:                              As pump/injector

Posttime                      
Posttime Mode:                                           Off

Timetable                     




Instrument Curves
Store Board Temperature:                                   No
Store Optical Unit Temperature:                            No
Store UV Lamp Anode Voltage:                               No






=====================================================================
                             Column Comp.
=====================================================================

Column Comp. (G1316A)
=====================

Valve Position:                     Position 2 (Port 1 -> 6)

Left Temperature Control      
Temperature Control Mode:                    Temperature Set
Temperature:                                         60.0 °C

Enable Analysis Left Temperature
Enable Analysis Left Temperature On:                     Yes
Enable Analysis Left Temperature Value:              10.0 °C

Right Temperature Control     
Right temperature Control Mode:              Temperature Set
Right temperature:                                   60.0 °C

Enable Analysis Right Temperature
Enable Analysis Right Temperature On:                    Yes
Enable Analysis Right Temperature Value:             10.0 °C

Stop Time                     
Stoptime Mode:                              As pump/injector

Post Time                     
Posttime Mode:                                           Off

Timetable                     




Instrument Curves
Store Left Temperature:                                    No
Store Right Temperature:                                   No






=====================================================================
                               Sampler
=====================================================================

Sampler (G7129A)
================


Stop Time                     
Stoptime Mode:                              As pump/No limit

Post Time                     
Posttime Mode:                                           Off

Auxiliary                     
Draw Speed:                                       200 µL/min
Eject Speed:                                      400 µL/min
Needle Height Offset:                                 0.0 mm
Wait Time After Draw:                                  1.2 s

High throughput               
Sample Flush-Out Factor:                                 5.0
Injection Valve to Bypass for Delay Volume Reduction:     No

Overlapped Injection          
Overlapped Injection Mode:                               Off

Injection                     
Injection Mode:                   Injection with needle wash
Injection Volume:                                    5.00 µL

Needle Wash                   
Needle Wash Location:                             Flush Port
Wash Time:                                               3 s

Timetable                     








=====================================================================
                              Quat. Pump
=====================================================================

Quat. Pump (G7111B)
===================

Flow:                                           0.000 mL/min
Low Pressure Limit:                                10.00 bar
High Pressure Limit:                              600.00 bar
Maximum Flow Gradient:                       100.000 mL/min²
Primary Channel:                                   Automatic

Stroke                        
Automatic Stroke Calculation:                            Yes

Compress                      
Compressibility Mode:              Compressibility Value Set
Compressibility:                               100 10e-6/bar

Stop Time                     
Stoptime Mode:                                      Time set
Stoptime:                                          16.00 min

Post Time                     
Posttime Mode:                                           Off
Solvent Composition
   Channel           Name 1   Used   Percent
                                           %
--------------------------------------------
         A   H2O + 0.1 % FA    Yes      97.0
         B CH3CN + 0.1 % FA    Yes       3.0
         C                      No          
         D                      No          



Timetable
   Time    A    B   C   D   Flow   Pressure
    min    %    %   %   % mL/min        bar
-------------------------------------------
   0.01 97.0  3.0 0.0 0.0  0.500        ---
   0.60 97.0  3.0 0.0 0.0    ---        ---
  11.40 50.0 50.0 0.0 0.0  0.500        ---
  11.90 10.0 90.0 0.0 0.0  0.800        ---
  13.15 10.0 90.0 0.0 0.0  0.800        ---
  13.45 97.0  3.0 0.0 0.0  0.500        ---
  15.99 97.0  3.0 0.0 0.0  0.500        ---
  16.00 97.0  3.0 0.0 0.0  0.000        ---






Instrument Curves
Store Pressure:                                           Yes
Store Flow:                                                No
Store Solvent Ratio A:                                     No
Store Solvent Ratio B:                                    Yes
Store Solvent Ratio C:                                     No
Store Solvent Ratio D:                                     No
Store Direction of Piston A:                               No





=====================================================================
                       Mass Spectrometer Detector
=====================================================================


General Information
------- -----------

Use MSD                  : Enabled
Tune File                : atunes.tun
StopTime                 : asPump
Time Filter              : Enabled
Data Storage             : Full
Peakwidth                : 0.04 min
Fast Scan                : Disabled
Fast Scan Data Reconstruction: Disabled
Polarity Switch Delay    : 300 ms
Ionization Switch Delay  : 50 ms


Signals
-------

[Signal 1]

Ionization Mode          : API-ES
Polarity                 : Positive
Fragmentor Ramp          : Disabled
Percent Cycle Time       : 100.00 %

Scan Parameters

   Time |      Mass Range    |Frag- | Gain|Thres-| Step-
  (min) |    Low   |  High   |mentor| EMV | hold | size
 -------|----------|---------|------|-----|------|-------
    0.00           Not Active
    2.70    150.00   2000.00   150    1.0       0  0.20
   14.00           Not Active


[Signal 2]

Not Active


[Signal 3]

Not Active


[Signal 4]

Not Active



Spray Chamber
----- -------

[MSZones]

Gas Temp                 : 315 C                  maximum 350 C
DryingGas                : 12.0 l/min             maximum 13.0 l/min
Neb Pres                 : 60 psig                maximum 60 psig
Quad Temp                : 0 C                    maximum 0 C

VCap (Positive)          : 4000 V
VCap (Negative)          : 3000 V

[Time Table]

Time Table is empty.



                      END OF MS ACQUISITION PARAMETERS

=====================================================================
                              FIA Series
=====================================================================

FIA Series in this Method   :      Disabled

Time Setting
    Time between Injections   :     0.19 min
    Injection Loop Flush Time :     0.17 min




