CRC AI oligo list column annotations

"Numeric_ID" gRNA identifier
"Num_hits_MM" total number of bowtie hits
"offtarget.zhangscore" off-target score calculated using the formula in Sanjana et al. Nat. Methods, 2014 
"Num_hits_MM0" number of bowtie hits with 0 mismatches
"Num_hits_MM1" number of bowtie hits with 1 mismatch
"Num_hits_MM2" number of bowtie hits with 2 mismatches         
"Num_hits_MM3" number of bowtie hits with 3 mismatches 
"Sample_ID" gRNA target annotation
"sgRNA_seq" gRNA sequence
"Oligo_sequence" guide oligo sequence
"Num_off_targets" number of potential off-targets calculated based on the exact matches of 15 bp next to PAM
"Activity_score" on-target activity score calculated using a model in Xu et al. Genome Res., 2015        
"Chromosome" chromosome of the guide target, GRCh37/hg19 coordinates
"Start" start position of the guide target, GRCh37/hg19 coordinates
"End" end position of the guide target, GRCh37/hg19 coordinates
"Strand" strand of teh guide target, GRCh37/hg19 coordinates
"Target_name" name of the target gene
"Target_id" Ensembl version 75 identifier of the target gene            
"Target_type" the type of the gene according to Ensembl
"HasUMI" gRNA has a random sequence label (RSL)
"All_target_annotations" all targeted genome features overlapped by the gRNA target sequence 
"is.tss.guide" guide targets promoter instead of exon
"guide.num.GC" number of C and G bases in the guide sequence