Examination target	Examinated target organism	PCR method	Material	PCR-target-Region	Forward-Primer (5' - 3')	Reverse-Primer (5' - 3')	Annealing	Reference
1. Parasites	Apicomplexan parasites	conventional PCR	rRNA	18S rRNA	CCC ATG CAT GTC TAA GTA TAA GC	CAC TGC CAC GGT AGT CCA ATA C	58°C	3
1. Parasites	Apicomplexan parasites	conventional PCR	rRNA	28S rRNA	TAC CCG CTG AAC TTA AGC	CCA CCA AGA TCT GCA CTA G	58°C	3
1. Parasites	Haemosporidian parasites	conventional PCR	mtDNA	mitochondrial Cytochr. B gene	CAT ATA TTA AGA GAA ITA TGG AG	ATA GAA AGA TAA GAA ATA CCA TTC	58°C	4
1. Parasites	Plasmodidae/Haemoproteidae	nested PCR 1	mtDNA	mitochondrial Cytochr. B gene	ATG GTG CTT TCG ATA TAT GCA TG	GCA TTA TCT GGA TGT GAT AAT GGT	58°C	4
1. Parasites	Leucocytozoon	nested PCR 2	mtDNA	mitochondrial Cytochr. B gene	ATG GTG TTT TAG ATA CTT ACA TT	CAT TAT CTG GAT GAG ATA ATG GIG C	58°C	4
2. Viruses	Polyomavirus	consensus nested PCR 1	DNA	Virus protein 1	CCA GAC CCA ACT ARR AAT GAR AA	AAC AAG AGA CAC AAA TNT TTC CNC C	46°C	5
2. Viruses	Polyomavirus	consensus nested PCR 2	DNA	Virus protein 1	ATG AAA ATG GGG TTG GCC CNC TNT GYA ARG	CCC TCA TAA ACC CGA ACY TCY TCH ACY TG	56°C	5
2. Viruses	Circovirus	consensus nested PCR 1	DNA	Re-encoding region	AGA GGT GGG TCT TCA CNH TBA AYA A	AAG GCA GCC ACC CRT ARA ART CRT C	46°C	6
2. Viruses	Circovirus	consensus nested PCR 2	DNA	Re-encoding region	AGC AAG GAA CCC CTC AYY TBC ARG G	ACG ATG ACT TCN GTC TTS MAR TCA CG	56°C	6
2. Viruses	Bornavirus	consensus RT-PCR	RNA	Nucleocapsid gene	CCH CAT GAG GCT ATW GAT TGG ATT AAC G	GCM CGG TAG CCN GCC ATT GTD GG	48.5°C	7
2. Viruses	Bornavirus	consensus RT-PCR	RNA	Matrix gene	GGR CAA GGT AAT YGT YCC TGG ATG GCC	CCA ACA CCA ATG TTC CGA AGM CG	48.5°C	7
2. Viruses	Usutu virus	consensus RT-PCR	RNA	Nonstructural protein 1 gene	CGT TCT CGA CTT TGA CTA	GCT AGT AGT AGT TCT TAT GGA	58°C	8
2. Viruses	Avian leucosis virus	RT-PCR	RNA	polymerase gene	GGA TGA GGT GAC TAA GAA AG	GGG AGG TGG CTG ACT GTG T	48°C	9
3. Mycobacterial species and subspecies	Mycobacterium (M.) genus	conventional PCR	gDNA	16SrRNA gene	GAG AGT TTG ATC CTG GCT CAG	TGC ACA CAG GCC ACA AGG GA	60°C	10
3. Mycobacterial species and subspecies	Mycobacterium genavense	conventional PCR	gDNA	21 kDa protein	TGC ACG GCA ACA TCA AAG AC	CGC CGG AAT CTG CAT CAC	62°C	11
3. Mycobacterial species and subspecies	Mycobacterium avium 	conventional PCR	gDNA	IS1245	GCC GCC GAA ACG ATC TAC	AGG TGG CGT CGA GGA AGA C	66°C	12
3. Mycobacterial species and subspecies	Mycobacterium avium subsp. avium	conventional PCR	gDNA	IS901	GCA ACG GTT GTT GCT TGA AA	TGA TAC GGC CGG AAT CGC GT	60°C	13
3. Mycobacterial species and subspecies	Mycobacterium avium, not subsp. avium	conventional PCR	gDNA	FR300*	CAG CCA GCC GAA TGT CAT CC	CAA CTC GCG ACA CGT TCA CC	65°C	13, 14
4. MIRU-VNTR genotyping of Mycobacterium avium	Mycobacterium avium	conventional PCR	gDNA	Locus 292	CTT GAG CAG CTC GTA AAG CGT	GCT GTA TGA GGA AGT CTA TTC ATG G	58°C	15, 16, 17
4. MIRU-VNTR genotyping of Mycobacterium avium	Mycobacterium avium	conventional PCR	gDNA	Locus X3	AAC GAG AGG AAG AAC TAA GCC G	TTA CGG AGC AGG AAG GCC AGC GGG	62°C	15, 16, 17
4. MIRU-VNTR genotyping of Mycobacterium avium	Mycobacterium avium	conventional PCR	gDNA	Locus 25	GTC AAG GGA TCG GCG AGG	TGG ACT TGA GCA CGG TCA T	55°C	15, 16, 17
4. MIRU-VNTR genotyping of Mycobacterium avium	Mycobacterium avium	conventional PCR	gDNA	Locus 47	CGT TGC GAT TTC TGC GTA GC	GGT GAT GGT CGT GGT CAT CC	64°C	15, 16, 17
4. MIRU-VNTR genotyping of Mycobacterium avium	Mycobacterium avium	conventional PCR	gDNA	Locus 3	CAT ATC TGG CAT GGC TCC AG	ATC GTG TTG ACC CCA AAG AAA T	60°C	15, 16, 17
4. MIRU-VNTR genotyping of Mycobacterium avium	Mycobacterium avium	conventional PCR	gDNA	Locus 7	GAC AAC GAA ACC TAC CTC GTC	GTG AGC TGG CGG CCT AAC	60°C	15, 16, 17
4. MIRU-VNTR genotyping of Mycobacterium avium	Mycobacterium avium	conventional PCR	gDNA	Locus 10	GAC GAG CAG CTG TCC GAG	GAG AGC GTG GCC ATC GAG	54°C	15, 16
4. MIRU-VNTR genotyping of Mycobacterium avium	Mycobacterium avium	conventional PCR	gDNA	Locus 32	CCA CAG GGT TTT TGG TGA AG	GGA AAT CCA ACA GCA AGG AC	57°C	15, 16, 17