library(Signac) library(Seurat) library(GenomicRanges) library(future) library(EnsDb.Mmusculus.v79) library(JASPAR2020) library(TFBSTools) library(BSgenome.Mmusculus.UCSC.mm10) library(patchwork) set.seed(1234)
In [2]:
BiocManager::install("JASPAR2020")

'getOption("repos")' replaces Bioconductor standard repositories, see
'help("repositories", package = "BiocManager")' for details.
Replacement repositories:
    CRAN: https://cran.r-project.org
In [ ]:
plan("multiprocess", workers = 20)
options(future.globals.maxSize = 50000 * 1024^2) # for 50 Gb RAM
In [3]:
# read in peak sets
peaks.mo1 <- read.table(
  file = path+"230117_ATAC/MO1/outs/peaks.bed",
  col.names = c("chr", "start", "end")
)
peaks.mo2 <- read.table(
  file = path+"230117_ATAC/MO2/outs/peaks.bed",
  col.names = c("chr", "start", "end")
)
peaks.my1 <- read.table(
  file = path+"230117_ATAC/MY1/outs/peaks.bed",
  col.names = c("chr", "start", "end")
)
peaks.my2 <- read.table(
  file = path+"230117_ATAC/MY2/outs/peaks.bed",
  col.names = c("chr", "start", "end")
)
In [5]:
# convert to genomic ranges
gr.mo1 <- makeGRangesFromDataFrame(peaks.mo1)
gr.mo2 <- makeGRangesFromDataFrame(peaks.mo2)
gr.my1 <- makeGRangesFromDataFrame(peaks.my1)
gr.my2 <- makeGRangesFromDataFrame(peaks.my2)
In [6]:
# Create a unified set of peaks to quantify in each dataset
combined.peaks <- reduce(x = c(gr.mo1, gr.mo2, gr.my1, gr.my2))

# Filter out bad peaks based on length
peakwidths <- width(combined.peaks)
combined.peaks <- combined.peaks[peakwidths  < 10000 & peakwidths > 20]
combined.peaks

GRanges object with 250407 ranges and 0 metadata columns:
             seqnames          ranges strand
                <Rle>       <IRanges>  <Rle>
       [1]       chr1 3119286-3120251      *
       [2]       chr1 3121083-3121994      *
       [3]       chr1 3268133-3269006      *
       [4]       chr1 3445548-3446463      *
       [5]       chr1 3476940-3477877      *
       ...        ...             ...    ...
  [250403] JH584304.1     72703-73643      *
  [250404] JH584304.1     89972-90911      *
  [250405] JH584304.1     97685-98591      *
  [250406] JH584304.1   103126-104027      *
  [250407] JH584304.1   106364-107253      *
  -------
  seqinfo: 30 sequences from an unspecified genome; no seqlengths
In [7]:
# load metadata
md.mo1 <- read.table(
  file = path+"230117_ATAC/MO1/outs/singlecell.csv",
  stringsAsFactors = FALSE,
  sep = ",",
  header = TRUE,
  row.names = 1
)[-1, ] # remove the first row

md.mo2 <- read.table(
  file = path+"230117_ATAC/MO2/outs/singlecell.csv",
  stringsAsFactors = FALSE,
  sep = ",",
  header = TRUE,
  row.names = 1
)[-1, ]

md.my1 <- read.table(
  file = path+"230117_ATAC/MY1/outs/singlecell.csv",
  stringsAsFactors = FALSE,
  sep = ",",
  header = TRUE,
  row.names = 1
)[-1, ]

md.my2 <- read.table(
  file = path+"230117_ATAC/MY2/outs/singlecell.csv",
  stringsAsFactors = FALSE,
  sep = ",",
  header = TRUE,
  row.names = 1
)[-1, ]
In [8]:
# perform an initial filtering of low count cells
md.mo1 <- md.mo1[md.mo1$passed_filters > 500, ]
md.mo2 <- md.mo2[md.mo2$passed_filters > 500, ]
md.my1 <- md.my1[md.my1$passed_filters > 500, ]
md.my2 <- md.my2[md.my2$passed_filters > 500, ]
In [9]:
# create fragment objects
frags.mo1 <- CreateFragmentObject(
  path = path+"230117_ATAC/MO1/outs/atac_fragments.tsv.gz",
  cells = rownames(md.mo1)
)

frags.mo2 <- CreateFragmentObject(
  path = path+"230117_ATAC/MO2/outs/atac_fragments.tsv.gz",
  cells = rownames(md.mo2)
)
## Computing hash
frags.my1 <- CreateFragmentObject(
  path = path+"230117_ATAC/MY1/outs/atac_fragments.tsv.gz",
  cells = rownames(md.my1)
)
## Computing hash
frags.my2 <- CreateFragmentObject(
  path = path+"230117_ATAC/MY2/outs/atac_fragments.tsv.gz",
  cells = rownames(md.my2)
)
## Computing hash

Computing hash

Computing hash

Computing hash

Computing hash
In [10]:
pbmcmo1.counts <- FeatureMatrix(
  fragments = frags.mo1,
  features = combined.peaks,
  cells = rownames(md.mo1)
)

pbmcmo2.counts <- FeatureMatrix(
  fragments = frags.mo2,
  features = combined.peaks,
  cells = rownames(md.mo2)
)

pbmcmy1.counts <- FeatureMatrix(
  fragments = frags.my1,
  features = combined.peaks,
  cells = rownames(md.my1)
)

pbmcmy2.counts <- FeatureMatrix(
  fragments = frags.my2,
  features = combined.peaks,
  cells = rownames(md.my2)
)

Extracting reads overlapping genomic regions

Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."

R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call

Extracting reads overlapping genomic regions

Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."

R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call

Extracting reads overlapping genomic regions

Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."

R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call

Extracting reads overlapping genomic regions

Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."
Warning message:
"Detected creation of a 'multiprocess' future. Strategy 'multiprocess' is deprecated in future (>= 1.20.0) [2020-10-30]. Instead, explicitly specify either 'multisession' (recommended) or 'multicore'. In the current R session, 'multiprocess' equals 'multicore'."

R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
R_zmq_msg_send errno: 4 strerror: Interrupted system call
In [11]:
pbmcmo1_assay <- CreateChromatinAssay(pbmcmo1.counts, fragments = frags.mo1)
pbmcmo1 <- CreateSeuratObject(pbmcmo1_assay, assay = "ATAC", meta.data=md.mo1)

pbmcmo2_assay <- CreateChromatinAssay(pbmcmo2.counts, fragments = frags.mo2)
pbmcmo2 <- CreateSeuratObject(pbmcmo2_assay, assay = "ATAC", meta.data=md.mo2)

pbmcmy1_assay <- CreateChromatinAssay(pbmcmy1.counts, fragments = frags.my1)
pbmcmy1 <- CreateSeuratObject(pbmcmy1_assay, assay = "ATAC", meta.data=md.my1)

pbmcmy2_assay <- CreateChromatinAssay(pbmcmy2.counts, fragments = frags.my2)
pbmcmy2 <- CreateSeuratObject(pbmcmy2_assay, assay = "ATAC", meta.data=md.my2)

Warning message:
"Keys should be one or more alphanumeric characters followed by an underscore, setting key from atac to atac_"
Warning message:
"Keys should be one or more alphanumeric characters followed by an underscore, setting key from atac to atac_"
Warning message:
"Keys should be one or more alphanumeric characters followed by an underscore, setting key from atac to atac_"
Warning message:
"Keys should be one or more alphanumeric characters followed by an underscore, setting key from atac to atac_"
In [12]:
# add information to identify dataset of origin
pbmcmo1$dataset <- 'mo1'
pbmcmo2$dataset <- 'mo2'
pbmcmy1$dataset <- 'my1'
pbmcmy2$dataset <- 'my2'
In [13]:
pfm <- get(
  x = JASPAR2020,
  opts = list(collection = "CORE", tax_group = 'vertebrates', all_versions = FALSE)
)

Error in getMatrixSet(x = JASPAR2020, opts = list(collection = "CORE", : could not find function "getMatrixSet"
Traceback:
In [37]:
# merge all datasets, adding a cell ID to make sure cell names are unique
combined <- merge(
  x = pbmcmo2, 
  y = list(pbmcmy1, pbmcmy2),
  add.cell.ids = c("mo2", "my1", "my2")
)
combined[["ATAC"]]

ChromatinAssay data with 218763 features for 22377 cells
Variable features: 0 
Genome: 
Annotation present: FALSE 
Motifs present: FALSE 
Fragment files: 3
In [38]:
combined <- RunTFIDF(combined)
combined <- FindTopFeatures(combined, min.cutoff = 20)
combined <- RunSVD(combined)
combined <- RunUMAP(combined, dims = 2:50, reduction = 'lsi')

Performing TF-IDF normalization

Running SVD

Scaling cell embeddings

Warning message:
"The default method for RunUMAP has changed from calling Python UMAP via reticulate to the R-native UWOT using the cosine metric
To use Python UMAP via reticulate, set umap.method to 'umap-learn' and metric to 'correlation'
This message will be shown once per session"
11:25:58 UMAP embedding parameters a = 0.9922 b = 1.112

11:25:58 Read 22377 rows and found 49 numeric columns

11:25:58 Using Annoy for neighbor search, n_neighbors = 30

11:25:58 Building Annoy index with metric = cosine, n_trees = 50

0%   10   20   30   40   50   60   70   80   90   100%

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11:26:01 Writing NN index file to temp file /tmp/Rtmp2X56oP/file1379234d6d9cb

11:26:01 Searching Annoy index using 1 thread, search_k = 3000

11:26:08 Annoy recall = 100%

11:26:09 Commencing smooth kNN distance calibration using 1 thread
 with target n_neighbors = 30

11:26:11 Initializing from normalized Laplacian + noise (using irlba)

11:26:14 Commencing optimization for 200 epochs, with 948986 positive edges

11:26:26 Optimization finished
In [40]:
combined <- FindNeighbors(object = combined, reduction = 'lsi', dims = 2:30)
combined <- FindClusters(object = combined, resolution = 1.0, verbose = FALSE, algorithm = 3)

Computing nearest neighbor graph

Computing SNN
In [ ]:
In [41]:
DimPlot(combined)
In [60]:
# quantify gene activity
gene.activities <- GeneActivity(rds, features = VariableFeatures(rna))

Extracting gene coordinates

Extracting reads overlapping genomic regions

Extracting reads overlapping genomic regions

Extracting reads overlapping genomic regions
In [61]:
rds[["ACTIVITY"]] <- CreateAssayObject(counts = gene.activities)
In [62]:
DefaultAssay(rds) <- "ACTIVITY"
rds <- NormalizeData(rds)
rds <- ScaleData(rds, features = rownames(rds))

Centering and scaling data matrix
In [98]:
transfer.anchors <- FindTransferAnchors(reference = rna, query = rds, features = VariableFeatures(object = rna),
    reference.assay = "RNA", query.assay = "ACTIVITY", reduction = "cca")

Warning message:
"536 features of the features specified were not present in both the reference query assays. 
Continuing with remaining 1464 features."
Running CCA

Merging objects

Finding neighborhoods

Finding anchors

	Found 25562 anchors
In [69]:
unique(rna@meta.data$annotation_publish_1)
  1. T
  2. Cholangiocyte
  3. B
  4. DC
  5. NK
  6. Macrophage
  7. Endothelial
  8. Mesenchymal
  9. Monocyte
  10. Mast
  11. Neutrophil
  12. Hepatocyte
Levels:
  1. 'B'
  2. 'Cholangiocyte'
  3. 'DC'
  4. 'Endothelial'
  5. 'Hepatocyte'
  6. 'Macrophage'
  7. 'Mast'
  8. 'Mesenchymal'
  9. 'Monocyte'
  10. 'NK'
  11. 'Neutrophil'
  12. 'T'
In [99]:
celltype.predictions <- TransferData(anchorset = transfer.anchors, refdata = rna$subannotation_publish,
    weight.reduction = rds[["lsi"]], dims = 2:50)

Finding integration vectors

Finding integration vector weights

Predicting cell labels
In [100]:
rds <- AddMetaData(rds, metadata = celltype.predictions)
In [24]:
DimPlot(rds, group.by = "anno_publish", label = TRUE) + NoLegend()
In [90]:
FeaturePlot(rds,  features = c('Ttr'))
In [24]:
saveRDS(rds, '/home/kytak/kwonyongtak/02_aging/write/230117_ATAC/MO2_Y1_2_merged_annotated.rds')
In [1]:
rds <- readRDS('/home/kytak/kwonyongtak/02_aging/write/230117_ATAC/MO2_Y1_2_merged_annotated.rds')
In [18]:
rds

An object of class Seurat 
251869 features across 22377 samples within 2 assays 
Active assay: ATAC (250407 features, 249035 variable features)
 2 layers present: counts, data
 1 other assay present: ACTIVITY
 2 dimensional reductions calculated: lsi, umap
In [21]:
colnames(rds@meta.data)
  1. 'orig.ident'
  2. 'nCount_ATAC'
  3. 'nFeature_ATAC'
  4. 'total'
  5. 'duplicate'
  6. 'chimeric'
  7. 'unmapped'
  8. 'lowmapq'
  9. 'mitochondrial'
  10. 'nonprimary'
  11. 'passed_filters'
  12. 'is__cell_barcode'
  13. 'excluded_reason'
  14. 'TSS_fragments'
  15. 'DNase_sensitive_region_fragments'
  16. 'enhancer_region_fragments'
  17. 'promoter_region_fragments'
  18. 'on_target_fragments'
  19. 'blacklist_region_fragments'
  20. 'peak_region_fragments'
  21. 'peak_region_cutsites'
  22. 'dataset'
  23. 'nCount_ACTIVITY'
  24. 'nFeature_ACTIVITY'
  25. 'predicted.id'
  26. 'prediction.score.Cd8_Tcf7.GZMK..Pdcd1..Early_activated.'
  27. 'prediction.score.Cholangiocyte'
  28. 'prediction.score.B_Sell.'
  29. 'prediction.score.Cd8_Tex'
  30. 'prediction.score.Cd4_NKT'
  31. 'prediction.score.cDC1'
  32. 'prediction.score.Cd4_Lef1.Sell.Actn1.'
  33. 'prediction.score.NK._Lilr.'
  34. 'prediction.score.Cd8_Tcf7.Ccr7..Tnfrsf9.4..naive.'
  35. 'prediction.score.cDC2'
  36. 'prediction.score.Transitioning'
  37. 'prediction.score.T_Double_Negative'
  38. 'prediction.score.KC'
  39. 'prediction.score.Cd4_Tex'
  40. 'prediction.score.B_Cd80.'
  41. 'prediction.score.Cd8_Tcf7.GZMK..EM.'
  42. 'prediction.score.Cd4_Tox.Slamf6.Cxcr5..FH.'
  43. 'prediction.score.Endothelial_Fibrotic'
  44. 'prediction.score.NK_Klra.'
  45. 'prediction.score.Fibroblast_Pi16.'
  46. 'prediction.score.VSMC_Il.6.high'
  47. 'prediction.score.Endothelial_PV'
  48. 'prediction.score.Endothelial_Midzone'
  49. 'prediction.score.Cd8_Lef1.Sell.Actn1.'
  50. 'prediction.score.Fibroblast_Smoc1.'
  51. 'prediction.score.Endothelial_CV'
  52. 'prediction.score.Fibroblast_Mmp3.'
  53. 'prediction.score.NK._H2'
  54. 'prediction.score.Cd4_Ifnr.Fasl..H1.'
  55. 'prediction.score.Monocyte'
  56. 'prediction.score.B_Plasma'
  57. 'prediction.score.Mast'
  58. 'prediction.score.Patrolling.Monocyte'
  59. 'prediction.score.Endothelial_Lymphatic'
  60. 'prediction.score.Neutrophil'
  61. 'prediction.score.Hepatocyte'
  62. 'prediction.score.Fibroblast_Wif1.'
  63. 'prediction.score.pDC'
  64. 'prediction.score.Treg'
  65. 'prediction.score.mDC'
  66. 'prediction.score.Fibroblast_Srgn.'
  67. 'prediction.score.VSMC_Il.6.low'
  68. 'prediction.score.γδT'
  69. 'prediction.score.Fibroblast_Col8a1.'
  70. 'prediction.score.HSC'
  71. 'prediction.score.Foam.Cells'
  72. 'prediction.score.max'
  73. 'anno_publish'
  74. 'annosimple'
  75. 'subannotation'
  76. 'age_sub'
  77. 'age_publish'
  78. 'annotation'
  79. 'T_subannotation'
  80. 'age_Tsub'
  81. 'age'
  82. 'anno_publish'
  83. 'annosimple'
  84. 'subannotation'
  85. 'age_sub'
  86. 'age_publish'
  87. 'annotation'
  88. 'T_subannotation'
  89. 'age_Tsub'
In [ ]:
keeps <- c('annosimple','age_publish')
DF[keeps]
In [15]:
DimPlot(rds, group.by = "annosimple", label = TRUE) + NoLegend()

peak calling

In [9]:
rds <- readRDS('/home/kytak/kwonyongtak/02_aging/write/230117_ATAC/MO2_Y1_2_merged_finalannotated.rds')
In [10]:
DimPlot(rds, group.by = 'subannotation')
In [9]:
rds@meta.data
A data.frame: 22377 × 94
orig.identnCount_ATACnFeature_ATACtotalduplicatechimericunmappedlowmapqmitochondrialnonprimary⋯endo_subATAC_snn_res.0.8ATAC_snn_res.1ATAC_snn_res.0.5ATAC_snn_res.1.5ATAC_snn_res.2ATAC_snn_res.3ATAC_snn_res.4subannotationannosimple
<fct><dbl><dbl><dbl><dbl><dbl><dbl><dbl><dbl><dbl>⋯<fct><fct><fct><fct><fct><fct><fct><fct><fct><fct>
mo2_AAACGAAAGATCGCCC-1SeuratProject14954110633479115141 03961260 9 4⋯2612131313129 6 T_Cd4 T
mo2_AAACGAAAGCAGTAGC-1SeuratProject22176162325483823520 05122855 71 2⋯0 21231825242323Mesenchymal_1Mesenchymal
mo2_AAACGAAAGTATACGA-1SeuratProject 7390 589318547 9112 0192 904 29 6⋯2612131313129 6 T_Cd4 T
mo2_AAACGAAAGTGATTAG-1SeuratProject 3718 315111031 5864 0131 535 98 0⋯265 7 5 5 3 4 9 T_Cd8 T
mo2_AAACGAACAACTAGAA-1SeuratProject25191158235879626046 26272375 5113⋯265 7 5 5 3 4 9 T_Cd8 T
mo2_AAACGAACAATTGTGC-1SeuratProject 470 420 7710 2353 3 871725 8 7⋯1414159 16164447Hepatocyte Hepatocyte
mo2_AAACGAACAGTTCGGC-1SeuratProject 4516 4105 9261 3182 1 96 427 19 0⋯494 1 4 12118 5 Monocyte Monocyte
mo2_AAACGAACATCCCTCA-1SeuratProject15126107574649925310 65202538 683 2⋯276 106 10132127B_2 B
mo2_AAACGAAGTCAGGCTC-1SeuratProject 6964 567414450 5668 0177 603 66 0⋯301 0 1 0 0 2 2 B_1 B
mo2_AAACGAAGTGTAGGAC-1SeuratProject 9127 787518690 6638 0166 765 36 0⋯464 1 4 9 8 4749MdM MdM
mo2_AAACGAAGTTAGGAGC-1SeuratProject13876 98533379914551 04151647 304 3⋯301 0 1 0 0 2 2 B_1 B
mo2_AAACGAAGTTCTTTGT-1SeuratProject 7523 616514855 4780 1177 774 141 8⋯3619212122303242T_Cd8 T
mo2_AAACGAATCATTCGGA-1SeuratProject16684124874706623842 05372149 55 0⋯0 21231825242323Mesenchymal_1Mesenchymal
mo2_AAACGAATCCATTGTT-1SeuratProject16107113384109818527 86901962 3 5⋯265 7 5 5 3 3721T_Cd8 T
mo2_AAACGAATCCGTCAAA-1SeuratProject12313 97265887940355 85103109 85 7⋯-123242226262725Monocyte Monocyte
mo2_AAACTCGAGAGGTCCA-1SeuratProject 514 505 684 10 0 21 44 13 0⋯276 106 10132159B_2 B
mo2_AAACTCGAGCGTAGCA-1SeuratProject 7323 56062336312719 33041311 151 0⋯301 0 1 7 4 2220B_1 B
mo2_AAACTCGAGGGACGTT-1SeuratProject11015 80543977624210 05761808 17 3⋯265 7 5 5 3 3721T_Cd8 T
mo2_AAACTCGAGGTGATGC-1SeuratProject11849 86952931214128 23921215 1 0⋯301 0 1 0 0 2 2 B_1 B
mo2_AAACTCGAGTCTGCTA-1SeuratProject 9996 75212617813259 2366 997 27 3⋯2612131313129 6 T_Cd4 T
mo2_AAACTCGAGTTGTCCA-1SeuratProject169861150652972271311568334241781 3⋯276 106 10222018B_2 B
mo2_AAACTCGCAAGCAACG-1SeuratProject 1564 1518 2343 228 1 33 118 8 0⋯4416181519214243MdM MdM
mo2_AAACTCGCAATTAGGA-1SeuratProject14549113184681925393 35752157 47 2⋯2220221124292935NK NK
mo2_AAACTCGCAATTGCCA-1SeuratProject31010181838075639032 59963192 190 1⋯2226291132343435NK NK
mo2_AAACTCGCACTCGCAG-1SeuratProject18395131674193817885 35211930 103 3⋯301 0 1 0 0 2 2 B_1 B
mo2_AAACTCGCATGGCCTG-1SeuratProject 1860 1776 2934 528 0 33 117 4 0⋯-11618151921107 MdM MdM
mo2_AAACTCGGTAAGCCGA-1SeuratProject 1057 1037 1669 188 0 26 102 13 0⋯4416181519214243MdM MdM
mo2_AAACTCGGTATCTAGC-1SeuratProject11500 87883213016660 04011513 010⋯3813141414141312T_Cd8 T
mo2_AAACTCGGTATTGTCG-1SeuratProject21196142347771847229 19823458 24 4⋯2618201721201716T_Cd4 T
mo2_AAACTCGGTCCCTTTG-1SeuratProject14928114016772546699 17522574 6212⋯4110118 8 7 7 4 cDC cDC
⋮⋮⋮⋮⋮⋮⋮⋮⋮⋮⋮⋱⋮⋮⋮⋮⋮⋮⋮⋮⋮⋮
my2_TTTGGCCTCTCGTGAA-1SeuratProject1698511038 80151 56379121314 2783106 5⋯7_103 2 3 1 181210Endothelial_1Endothelial
my2_TTTGGCCTCTTAGTGG-1SeuratProject228611686133469719178733426149129345132⋯14 27309 33353536Hepatocyte Hepatocyte
my2_TTTGGTTAGACCCTAT-1SeuratProject2836517852101844 60581 81479 4674 17 5⋯26 18201721201716T_Cd4 T
my2_TTTGGTTAGCGCACAA-1SeuratProject 489 373 5782 3423 1 80 947359 1⋯7_132 6 2 6 6 3 1 Endothelial_2Endothelial
my2_TTTGGTTAGCTGGAGT-1SeuratProject 3840 3004 18968 13386 2 284 880 84 9⋯17 22251928274850Neutrophil Neutrophil
my2_TTTGGTTAGGGTGTTG-1SeuratProject1404210149 69480 49137 01140 2124193 17⋯7_7 3 2 3 1 181210Endothelial_1Endothelial
my2_TTTGGTTAGTAGGTCG-1SeuratProject 1101 1020 3592 2109 0 44 139 0 0⋯30 7 8 2023252424B_1 B
my2_TTTGGTTCACGTTACA-1SeuratProject 193 193 1154 16 0 61 172 1 0⋯14 14159 16162626Hepatocyte Hepatocyte
my2_TTTGGTTCACGTTAGT-1SeuratProject 9510 727428829019455020366046179197136⋯14 27309 33353536Hepatocyte Hepatocyte
my2_TTTGGTTCATTAACGC-1SeuratProject 3349 3071 5491 1244 0 33 231 2 0⋯30 7 8 1 7 4 1817B_1 B
my2_TTTGGTTCATTAAGGA-1SeuratProject12714 9304 47410 29552 2 566 2028 16 1⋯30 1 0 1 0 0 3130B_1 B
my2_TTTGGTTGTAACGGCA-1SeuratProject11422 8233 35185 19985 0 550 1385 17 0⋯26 12131313129 6 T_Cd4 T
my2_TTTGGTTGTATCACAC-1SeuratProject1344510052 42558 24039 0 497 1703 2 2⋯34 9 9 7 15151514KC KC
my2_TTTGGTTTCCAAACCA-1SeuratProject 4556 3146 22303 14175 4 534 1431721 0⋯7_112 6 2 6 425354Endothelial_2Endothelial
my2_TTTGGTTTCCTATCCG-1SeuratProject1551110928 69998 47276 01228 2452194 6⋯7_103 2 3 1 9 1210Endothelial_1Endothelial
my2_TTTGGTTTCGACTCGG-1SeuratProject 6072 4958 12416 4730 1 220 561 47 6⋯17 22251928274346Neutrophil Neutrophil
my2_TTTGGTTTCGATCGCG-1SeuratProject 4705 3838 24356 16756 7 454 1276194 4⋯7_110 3 0 2 2 1 0 Endothelial_2Endothelial
my2_TTTGTGTAGACGCCAA-1SeuratProject 3778 3242 11691 6775 2 215 400 0 0⋯7_120 3 0 2 2 1 0 Endothelial_2Endothelial
my2_TTTGTGTAGGAGTACC-1SeuratProject1980014143 74364 45348 8 829 2214425 8⋯12 17191620234141CholangiocyteCholangiocyte
my2_TTTGTGTAGGTTTCTG-1SeuratProject 541 534 1008 221 0 55 33 0 0⋯8 22251928274346Neutrophil Neutrophil
my2_TTTGTGTCAACTCAAC-1SeuratProject 3873 3324 11976 6425 1 170 531 73 2⋯7_120 3 0 2 2 1 0 Endothelial_2Endothelial
my2_TTTGTGTCAGTTAAAG-1SeuratProject 1616 1341 9242 4949 2 232 927131 1⋯34 9 9 7 29313031KC KC
my2_TTTGTGTCATACCCGG-1SeuratProject 5259 4284 68479 58359 0 907 2782204 0⋯7_110 3 0 2 2 1 0 Endothelial_2Endothelial
my2_TTTGTGTCATCAGAAA-1SeuratProject 9083 7436 51715 30725 5 637 3968 68 19⋯3 29322635373955Mesenchymal_3Mesenchymal
my2_TTTGTGTCATTAGCAC-1SeuratProject 1433 1375 3266 490 1 43 244 9 1⋯3 29322635373955Mesenchymal_3Mesenchymal
my2_TTTGTGTGTACAACGG-1SeuratProject 723 704 1062 91 0 7 55 8 0⋯34 9 9 7 29313031KC KC
my2_TTTGTGTGTAGAAAGG-1SeuratProject 6870 5724 14523 4899 0 176 639121 1⋯34 9 9 7 15151514KC KC
my2_TTTGTGTGTATGTCCA-1SeuratProject1683312060 63064 39343 71018 1921202 2⋯7_103 2 3 1 9 118 Endothelial_1Endothelial
my2_TTTGTGTTCCCAGCAG-1SeuratProject 3227 2933 4658 662 0 53 181 0 1⋯30 7 8 1 7 4 1817B_1 B
my2_TTTGTGTTCCGTGCAG-1SeuratProject2246314098 91573 58925121493 3836 0 5⋯30 1 0 1 0 0 5456B_1 B
In [6]:
rds

An object of class Seurat 
250407 features across 22377 samples within 1 assay 
Active assay: ATAC (250407 features, 0 variable features)
 2 dimensional reductions calculated: pca, umap
In [ ]:
In [2]:
atac = readRDS('/home/kytak/kwonyongtak/02_aging/write/230117_ATAC/MO2_Y1_2_final.rds')
In [3]:
atac

An object of class Seurat 
240571 features across 22377 samples within 2 assays 
Active assay: ATAC (218763 features, 218604 variable features)
 1 other assay present: RNA
 2 dimensional reductions calculated: lsi, umap
In [36]:
genomicanno <- ClosestFeature(
  object = atac,
  regions = granges(atac)
)

Warning message in ClosestFeature(object = atac, regions = granges(atac)):
"The following seqlevels present in query regions are not present
 in the supplied gene annotations and will be removed: GL456210.1, GL456211.1, GL456212.1, GL456216.1, GL456221.1, GL456233.1, JH584292.1, JH584304.1"
In [37]:
write.csv(genomicanno, "/home/kytak/kwonyongtak/02_aging/write/230117_ATAC/genomicanno.csv", row.names=FALSE)
In [ ]:
In [ ]:
In [ ]:
In [ ]:
In [ ]:
In [6]:
distance

Hits object with 218671 hits and 1 metadata column:
           queryHits subjectHits |  distance
           <integer>   <integer> | <integer>
       [1]         1     1532334 |     85649
       [2]         2     1532334 |     83906
       [3]         3     1588724 |         0
       [4]         4     1588725 |         0
       [5]         5     1588725 |         0
       ...       ...         ... .       ...
  [218667]    218667      850191 |         0
  [218668]    218668      850191 |         0
  [218669]    218669      850191 |         0
  [218670]    218670      850191 |         0
  [218671]    218671      854580 |      9420
  -------
  queryLength: 218763 / subjectLength: 1763965
In [ ]:
In [19]:
CoveragePlot(
  object = atac,
  group.by = 'dataset',
  region = "chr14-99700000-99760000"
)

rds@meta.data

Error in FUN(X[[i]], ...): Requested assay is not a ChromatinAssay.
Traceback:

1. CoveragePlot(object = atac, group.by = "dataset", region = "chr14-99700000-99760000")
2. lapply(X = seq_along(region), FUN = function(x) {
 .     SingleCoveragePlot(object = object, region = region[[x]], 
 .         features = features, expression.assay = expression.assay, 
 .         expression.slot = expression.slot, show.bulk = show.bulk, 
 .         annotation = annotation, peaks = peaks, peaks.group.by = peaks.group.by, 
 .         ranges = ranges, ranges.group.by = ranges.group.by, ranges.title = ranges.title, 
 .         region.highlight = region.highlight, assay = assay, split.assays = split.assays, 
 .         assay.scale = assay.scale, links = links, tile = tile, 
 .         tile.size = tile.size, tile.cells = tile.cells, bigwig = bigwig, 
 .         bigwig.type = bigwig.type, bigwig.scale = bigwig.scale, 
 .         group.by = group.by, split.by = split.by, window = window, 
 .         ymax = ymax, scale.factor = scale.factor, extend.upstream = extend.upstream, 
 .         extend.downstream = extend.downstream, cells = cells, 
 .         idents = idents, sep = sep, heights = heights, max.downsample = max.downsample, 
 .         downsample.rate = downsample.rate)
 . })
3. FUN(X[[i]], ...)
4. SingleCoveragePlot(object = object, region = region[[x]], features = features, 
 .     expression.assay = expression.assay, expression.slot = expression.slot, 
 .     show.bulk = show.bulk, annotation = annotation, peaks = peaks, 
 .     peaks.group.by = peaks.group.by, ranges = ranges, ranges.group.by = ranges.group.by, 
 .     ranges.title = ranges.title, region.highlight = region.highlight, 
 .     assay = assay, split.assays = split.assays, assay.scale = assay.scale, 
 .     links = links, tile = tile, tile.size = tile.size, tile.cells = tile.cells, 
 .     bigwig = bigwig, bigwig.type = bigwig.type, bigwig.scale = bigwig.scale, 
 .     group.by = group.by, split.by = split.by, window = window, 
 .     ymax = ymax, scale.factor = scale.factor, extend.upstream = extend.upstream, 
 .     extend.downstream = extend.downstream, cells = cells, idents = idents, 
 .     sep = sep, heights = heights, max.downsample = max.downsample, 
 .     downsample.rate = downsample.rate)
5. lapply(X = assay, FUN = function(x) {
 .     if (!inherits(x = object[[x]], what = "ChromatinAssay")) {
 .         stop("Requested assay is not a ChromatinAssay.")
 .     }
 . })
6. FUN(X[[i]], ...)
7. stop("Requested assay is not a ChromatinAssay.")
In [23]:
DefaultAssay(rds) <- 'ATAC'
In [12]:
Idents(rds) <- 'subannotation'
In [13]:
da_peaks <- FindMarkers(
  object = rds,
  ident.1 = "Mesenchymal_1",
  ident.2 = "Mesenchymal_2",
  test.use = 'LR',
  latent.vars = 'peak_region_fragments'
)

head(da_peaks)

Warning message:
"glm.fit: fitted probabilities numerically 0 or 1 occurred"
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Warning message:
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"glm.fit: fitted probabilities numerically 0 or 1 occurred"
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"glm.fit: fitted probabilities numerically 0 or 1 occurred"
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"glm.fit: fitted probabilities numerically 0 or 1 occurred"
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"glm.fit: fitted probabilities numerically 0 or 1 occurred"
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"glm.fit: fitted probabilities numerically 0 or 1 occurred"
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"glm.fit: fitted probabilities numerically 0 or 1 occurred"
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"glm.fit: fitted probabilities numerically 0 or 1 occurred"
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"glm.fit: fitted probabilities numerically 0 or 1 occurred"
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"glm.fit: fitted probabilities numerically 0 or 1 occurred"
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"glm.fit: fitted probabilities numerically 0 or 1 occurred"
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"glm.fit: fitted probabilities numerically 0 or 1 occurred"
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"glm.fit: fitted probabilities numerically 0 or 1 occurred"
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"glm.fit: fitted probabilities numerically 0 or 1 occurred"
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"glm.fit: fitted probabilities numerically 0 or 1 occurred"
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"glm.fit: fitted probabilities numerically 0 or 1 occurred"
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Warning message:
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"glm.fit: fitted probabilities numerically 0 or 1 occurred"
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"glm.fit: fitted probabilities numerically 0 or 1 occurred"
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"glm.fit: fitted probabilities numerically 0 or 1 occurred"
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"glm.fit: fitted probabilities numerically 0 or 1 occurred"
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"glm.fit: fitted probabilities numerically 0 or 1 occurred"
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"glm.fit: fitted probabilities numerically 0 or 1 occurred"
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"glm.fit: fitted probabilities numerically 0 or 1 occurred"
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"glm.fit: fitted probabilities numerically 0 or 1 occurred"
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"glm.fit: fitted probabilities numerically 0 or 1 occurred"
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"glm.fit: fitted probabilities numerically 0 or 1 occurred"
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"glm.fit: fitted probabilities numerically 0 or 1 occurred"
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"glm.fit: fitted probabilities numerically 0 or 1 occurred"
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"glm.fit: fitted probabilities numerically 0 or 1 occurred"
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"glm.fit: fitted probabilities numerically 0 or 1 occurred"
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"glm.fit: fitted probabilities numerically 0 or 1 occurred"
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"glm.fit: fitted probabilities numerically 0 or 1 occurred"
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A data.frame: 6 × 5
p_valavg_log2FCpct.1pct.2p_val_adj
<dbl><dbl><dbl><dbl><dbl>
JH584304.1-9520-105982.941026e-105 1.30093100.7760.0617.364534e-100
chr4-130036742-130037738 5.845488e-93-1.64486300.0080.536 1.463751e-87
chr7-6727063-6729079 2.235095e-75-1.16987630.0100.464 5.596836e-70
chr18-21299892-21300811 1.334723e-74-0.98168120.0810.576 3.342239e-69
chr1-82290952-82292071 1.166209e-66-1.10511270.0140.406 2.920269e-61
chr5-75734023-75735246 5.130574e-66-1.46339310.0000.371 1.284732e-60
In [19]:
meta <- read.csv('/home/kytak/kwonyongtak/02_aging/230117_ATAC/atacobs.csv', row.names = "X")
In [20]:
meta
A data.frame: 22377 × 104
orig.identnCount_ATACnFeature_ATACtotalduplicatechimericunmappedlowmapqmitochondrialnonprimary⋯ageage_simpleanno_publishage_publishall_accessibilityage_subleiden_3.0leiden_4.0leiden_6.0leiden_5.0
<chr><int><int><int><int><int><int><int><int><int>⋯<chr><chr><chr><chr><dbl><chr><int><int><int><int>
mo2_AAACGAAAGATCGCCC-1SeuratProject14954110633479115141 03961260 9 4⋯OldOld_T T Old_T 0.091208704Old_T_Cd4 2753 54 56
mo2_AAACGAAAGCAGTAGC-1SeuratProject22176162325483823520 05122855 71 2⋯OldOld_MesenchymalFibroblastOld_Fibroblast0.139958397Old_Mesenchymal_12926 13 24
mo2_AAACGAAAGTATACGA-1SeuratProject 7390 589318547 9112 0192 904 29 6⋯OldOld_T T Old_T 0.055226125Old_T_Cd4 2440 11 15
mo2_AAACGAAAGTGATTAG-1SeuratProject 3718 315111031 5864 0131 535 98 0⋯OldOld_T T Old_T 0.035410549Old_T_Cd8 3435 52 13
mo2_AAACGAACAACTAGAA-1SeuratProject25191158235879626046 26272375 5113⋯OldOld_T T Old_T 0.109321475Old_T_Cd8 6678 98 90
mo2_AAACGAACAATTGTGC-1SeuratProject 470 420 7710 2353 3 871725 8 7⋯OldOld_Hepatocyte HepatocyteOld_Hepatocyte0.011335646Old_Hepatocyte 2114 55 7
mo2_AAACGAACAGTTCGGC-1SeuratProject 4516 4105 9261 3182 1 96 427 19 0⋯OldOld_Monocyte Monocyte Old_Monocyte 0.052277688Old_Monocyte 3837 31 36
mo2_AAACGAACATCCCTCA-1SeuratProject15126107574649925310 65202538 683 2⋯OldOld_B B Old_B 0.086367041Old_B_2 4449 56 46
mo2_AAACGAAGTCAGGCTC-1SeuratProject 6964 567414450 5668 0177 603 66 0⋯OldOld_B B Old_B 0.052462954Old_B_1 3 5 0 9
mo2_AAACGAAGTGTAGGAC-1SeuratProject 9127 787518690 6638 0166 765 36 0⋯OldOld_MdM MdM Old_MdM 0.089529805Old_MdM 524 27 27
mo2_AAACGAAGTTAGGAGC-1SeuratProject13876 98533379914551 04151647 304 3⋯OldOld_B B Old_B 0.078043289Old_B_1 112 1 11
mo2_AAACGAAGTTCTTTGT-1SeuratProject 7523 616514855 4780 1177 774 141 8⋯OldOld_T T Old_T 0.058747031Old_T_Cd8 5250 61 48
mo2_AAACGAATCATTCGGA-1SeuratProject16684124874706623842 05372149 55 0⋯OldOld_MesenchymalFibroblastOld_Fibroblast0.110541336Old_Mesenchymal_12926 13 24
mo2_AAACGAATCCATTGTT-1SeuratProject16107113384109818527 86901962 3 5⋯OldOld_T T Old_T 0.093593106Old_T_Cd8 1028 16 23
mo2_AAACGAATCCGTCAAA-1SeuratProject12313 97265887940355 85103109 85 7⋯OldOld_Monocyte Monocyte Old_Monocyte 0.091895327Old_Monocyte 11 4 4 8
mo2_AAACTCGAGAGGTCCA-1SeuratProject 514 505 684 10 0 21 44 13 0⋯OldOld_B B Old_B 0.009228935Old_B_2 17 9 29 5
mo2_AAACTCGAGCGTAGCA-1SeuratProject 7323 56062336312719 33041311 151 0⋯OldOld_B B Old_B 0.050695200Old_B_1 333 21 9
mo2_AAACTCGAGGGACGTT-1SeuratProject11015 80543977624210 05761808 17 3⋯OldOld_T T Old_T 0.068317831Old_T_Cd8 1028 16 23
mo2_AAACTCGAGGTGATGC-1SeuratProject11849 86952931214128 23921215 1 0⋯OldOld_B B Old_B 0.071373120Old_B_1 3 5 0 1
mo2_AAACTCGAGTCTGCTA-1SeuratProject 9996 75212617813259 2366 997 27 3⋯OldOld_T T Old_T 0.064289965Old_T_Cd4 1810 3 4
mo2_AAACTCGAGTTGTCCA-1SeuratProject169861150652972271311568334241781 3⋯OldOld_B B Old_B 0.087912619Old_B_2 1911 39 43
mo2_AAACTCGCAAGCAACG-1SeuratProject 1564 1518 2343 228 1 33 118 8 0⋯OldOld_MdM MdM Old_MdM 0.023035066Old_MdM 4766 66 66
mo2_AAACTCGCAATTAGGA-1SeuratProject14549113184681925393 35752157 47 2⋯OldOld_NK NK Old_NK 0.108747698Old_NK 3942 71 64
mo2_AAACTCGCAATTGCCA-1SeuratProject31010181838075639032 59963192 190 1⋯OldOld_NK NK Old_NK 0.122690171Old_NK 3942 71 64
mo2_AAACTCGCACTCGCAG-1SeuratProject18395131674193817885 35211930 103 3⋯OldOld_B B Old_B 0.101974390Old_B_1 6284110100
mo2_AAACTCGCATGGCCTG-1SeuratProject 1860 1776 2934 528 0 33 117 4 0⋯OldOld_MdM MdM Old_MdM 0.025249513Old_MdM 3132 49 50
mo2_AAACTCGGTAAGCCGA-1SeuratProject 1057 1037 1669 188 0 26 102 13 0⋯OldOld_MdM MdM Old_MdM 0.017574364Old_MdM 4766 66 66
mo2_AAACTCGGTATCTAGC-1SeuratProject11500 87883213016660 04011513 010⋯OldOld_T T Old_T 0.079767182Old_T_Cd8 916 12 42
mo2_AAACTCGGTATTGTCG-1SeuratProject21196142347771847229 19823458 24 4⋯OldOld_T T Old_T 0.113045238Old_T_Cd4 1810 3 4
mo2_AAACTCGGTCCCTTTG-1SeuratProject14928114016772546699 17522574 6212⋯OldOld_cDC cDC Old_cDC 0.104435839Old_cDC 2 1 18 2
⋮⋮⋮⋮⋮⋮⋮⋮⋮⋮⋮⋱⋮⋮⋮⋮⋮⋮⋮⋮⋮⋮
my2_TTTGGCCTCTCGTGAA-1SeuratProject1698511038 80151 56379121314 2783106 5⋯YoungYoung_Endothelial Endothelial Young_Endothelial 0.087016061Young_Endothelial_12836 921
my2_TTTGGCCTCTTAGTGG-1SeuratProject228611686133469719178733426149129345132⋯YoungYoung_Hepatocyte Hepatocyte Young_Hepatocyte 0.183642060Young_Hepatocyte 3534 5158
my2_TTTGGTTAGACCCTAT-1SeuratProject2836517852101844 60581 81479 4674 17 5⋯YoungYoung_T T Young_T 0.125900835Young_T_Cd4 678010397
my2_TTTGGTTAGCGCACAA-1SeuratProject 489 373 5782 3423 1 80 947359 1⋯YoungYoung_Endothelial Endothelial Young_Endothelial 0.007560416Young_Endothelial_2302710091
my2_TTTGGTTAGCTGGAGT-1SeuratProject 3840 3004 18968 13386 2 284 880 84 9⋯YoungYoung_Neutrophil Neutrophil Young_Neutrophil 0.032489270Young_Neutrophil 4851 9554
my2_TTTGGTTAGGGTGTTG-1SeuratProject1404210149 69480 49137 01140 2124193 17⋯YoungYoung_Endothelial Endothelial Young_Endothelial 0.088335715Young_Endothelial_12836 921
my2_TTTGGTTAGTAGGTCG-1SeuratProject 1101 1020 3592 2109 0 44 139 0 0⋯YoungYoung_B B Young_B 0.015398810Young_B_1 17 9 74 5
my2_TTTGGTTCACGTTACA-1SeuratProject 193 193 1154 16 0 61 172 1 0⋯YoungYoung_Hepatocyte Hepatocyte Young_Hepatocyte 0.005538412Young_Hepatocyte 2114 23 7
my2_TTTGGTTCACGTTAGT-1SeuratProject 9510 727428829019455020366046179197136⋯YoungYoung_Hepatocyte Hepatocyte Young_Hepatocyte 0.107285887Young_Hepatocyte 3534 5158
my2_TTTGGTTCATTAACGC-1SeuratProject 3349 3071 5491 1244 0 33 231 2 0⋯YoungYoung_B B Young_B 0.036090944Young_B_1 1320 512
my2_TTTGGTTCATTAAGGA-1SeuratProject12714 9304 47410 29552 2 566 2028 16 1⋯YoungYoung_B B Young_B 0.078006469Young_B_1 112 1 1
my2_TTTGGTTGTAACGGCA-1SeuratProject11422 8233 35185 19985 0 550 1385 17 0⋯YoungYoung_T T Young_T 0.065258808Young_T_Cd4 2440 1115
my2_TTTGGTTGTATCACAC-1SeuratProject1344510052 42558 24039 0 497 1703 2 2⋯YoungYoung_KC KC Young_KC 0.086990997Young_KC 1519 3033
my2_TTTGGTTTCCAAACCA-1SeuratProject 4556 3146 22303 14175 4 534 1431721 0⋯YoungYoung_Endothelial Endothelial Young_Endothelial 0.031794410Young_Endothelial_22015 3352
my2_TTTGGTTTCCTATCCG-1SeuratProject1551110928 69998 47276 01228 2452194 6⋯YoungYoung_Endothelial Endothelial Young_Endothelial 0.090401851Young_Endothelial_1 425 2421
my2_TTTGGTTTCGACTCGG-1SeuratProject 6072 4958 12416 4730 1 220 561 47 6⋯YoungYoung_Neutrophil Neutrophil Young_Neutrophil 0.047947764Young_Neutrophil 2617 210
my2_TTTGGTTTCGATCGCG-1SeuratProject 4705 3838 24356 16756 7 454 1276194 4⋯YoungYoung_Endothelial Endothelial Young_Endothelial 0.039642129Young_Endothelial_216 6 1022
my2_TTTGTGTAGACGCCAA-1SeuratProject 3778 3242 11691 6775 2 215 400 0 0⋯YoungYoung_Endothelial Endothelial Young_Endothelial 0.033910543Young_Endothelial_216 6 7022
my2_TTTGTGTAGGAGTACC-1SeuratProject1980014143 74364 45348 8 829 2214425 8⋯YoungYoung_CholangiocyteCholangiocyteYoung_Cholangiocyte0.136712506Young_Cholangiocyte4148 6461
my2_TTTGTGTAGGTTTCTG-1SeuratProject 541 534 1008 221 0 55 33 0 0⋯YoungYoung_Neutrophil Fibroblast Young_Neutrophil 0.009983188Young_Neutrophil 5872 9677
my2_TTTGTGTCAACTCAAC-1SeuratProject 3873 3324 11976 6425 1 170 531 73 2⋯YoungYoung_Endothelial Endothelial Young_Endothelial 0.034657195Young_Endothelial_216 6 7022
my2_TTTGTGTCAGTTAAAG-1SeuratProject 1616 1341 9242 4949 2 232 927131 1⋯YoungYoung_KC KC Young_KC 0.021022085Young_KC 2343 4647
my2_TTTGTGTCATACCCGG-1SeuratProject 5259 4284 68479 58359 0 907 2782204 0⋯YoungYoung_Endothelial Endothelial Young_Endothelial 0.044129591Young_Endothelial_216 6 1022
my2_TTTGTGTCATCAGAAA-1SeuratProject 9083 7436 51715 30725 5 637 3968 68 19⋯YoungYoung_Mesenchymal HSC Young_HSC 0.092090599Young_Mesenchymal_3546110292
my2_TTTGTGTCATTAGCAC-1SeuratProject 1433 1375 3266 490 1 43 244 9 1⋯YoungYoung_Mesenchymal HSC Young_HSC 0.024997247Young_Mesenchymal_35461 8884
my2_TTTGTGTGTACAACGG-1SeuratProject 723 704 1062 91 0 7 55 8 0⋯YoungYoung_KC KC Young_KC 0.012359071Young_KC 2346 4647
my2_TTTGTGTGTAGAAAGG-1SeuratProject 6870 5724 14523 4899 0 176 639121 1⋯YoungYoung_KC KC Young_KC 0.059717931Young_KC 1543 4545
my2_TTTGTGTGTATGTCCA-1SeuratProject1683312060 63064 39343 71018 1921202 2⋯YoungYoung_Endothelial Endothelial Young_Endothelial 0.101158679Young_Endothelial_1 425 2429
my2_TTTGTGTTCCCAGCAG-1SeuratProject 3227 2933 4658 662 0 53 181 0 1⋯YoungYoung_B B Young_B 0.033843491Young_B_1 1320 512
my2_TTTGTGTTCCGTGCAG-1SeuratProject2246314098 91573 58925121493 3836 0 5⋯YoungYoung_B B Young_B 0.101484522Young_B_1 121 619
In [21]:
rds@meta.data <- meta
In [22]:
DimPlot(rds, group.by = 'annotation')
In [14]:
DimPlot(rds, group.by = 'subannotation')