Published May 27, 2024 | Version v1
Dataset Open

PTGS is dispensable for the initiation of epigenetic silencing of an active transposon in Arabidopsis

  • 1. Gregor Mendel Institute of Molecular Plant Biology GmbH

Description

The dataset contains all the original raw files for RNA blots, qPCR, Sanger sequencing files… and any other type of source data, sorted by figure and figure panel. NGS data has been deposited on NCBI SRA, accession numbers of datasets used in each figure are listed accordingly in this document.

 

The content of each file is:

 

FIGURE 1:

-        1C: qPCR data for the estimation of EVD copy number at generations F2, F4 and F6 bulks in RDR6- and rdr6-EVD backgrounds.

-        1D: qPCR data for expression levels of EVD shGAG  at generations F2, F4 and F6 bulks in RDR6- and rdr6-EVD backgrounds.

-        1E: Northern blots raw TIFF image files for the detection of EVD-LTR and EVD-GAG derived siRNAs in RDR6- and rdr6-EVD backgrounds at F2, F4 and F6 generation bulks.

 

FIGURE 2:

-        2A: Northern blots raw TIFF image files for the detection of EVD-LTR and EVD-GAG derived siRNAs in RDR6- and rdr6-EVD F6 individuals.

-        2B: qPCR data for expression levels of EVD-GAG in in RDR6- and rdr6-EVD F6 individuals.

-        2C: qPCR data for the estimation of EVD copy number in RDR6- and rdr6-EVD F6 individuals.

 

FIGURE 5:

-        5A: qPCR data for the estimation of EVD copy number in bulks of NRPD1 and NRPE1 wild-types and mutants EVD lines at generations F2, F4 and F6.

-        5B: qPCR data for expression levels of EVD shGAG in bulks of NRPD1 and NRPE1 wild-type and mutant with active EVD at generations F2, F4 and F6.

-        5C: Northern blots raw TIFF image files for the detection of EVD-LTR and EVD-GAG derived siRNAs in bulks of 3 distinct lines of NRPD1- and nrpd1-EVD F6 generation.

-        5D: Northern blots raw TIFF image files for the detection of EVD-LTR and EVD-GAG derived siRNAs in bulks of 3 distinct lines of NRPE1- and nrpe1- EVD F6 generation.

-        5E+F: Bisulfite-PCR Sanger sequencing raw data for EVD-LTR DNA methylation analysis in NRPD1 and NRPE1 wild-type and mutant with active EVD at generations F6. (Also used in Supp Figure 5).

 

SUPP FIGURE 1:

-        qPCR data for the estimation of EVD copy number at generations F2, F4 and F6 bulks in RDR6- and rdr6-EVD backgrounds.

 

SUPP FIGURE 6:

-        Bisulfite-PCR Sanger sequencing raw data for EVD-3’GAG DNA methylation analysis in NRPD1 and NRPE1 wild-type and mutant with active EVD at generations F6.

 

NGS DATASETS:

 

All the NGS data generated for this study can be found under the SRA BioProject ID PRJNA1111825, Submission ID SUB14423611. The data was used to generate the following figures:

-        Figures 3, Figure 4 and Supplementary Figures 2, 3, 4 and 5

 

Files

Figure_1.zip

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