This dataset contains the raw blot, PCR and analyisis presented in Figure4.
A zip file was created to mantain the subfolders inside the main fiolder

Timecourse folder
In this folder there are the raw files for the timecourse experiment (figure 4A, B, C)
	WB: Western blot files
		PNG files:
		Blot1: red channel from licor blot showing Western blot for PPM1H WT MEFs for the three experimental replicates. tot Ub, tot LRRK2, tot Rab10 are in this channel
		Blot1_1: green channel from licor blot showing Western blot for PPM1H WT MEFs for the three experimental replicates. pSer65 Ub, pSer935 LRRK2, OPA1, PPM1H and pRab10  are in this channel.
		Blot2: Vinculin (green) and tubulin (red) Western blot for PPM1H WT MEFs for the three experimental replicates.
		TPS_bistris_trisgly: total protein stain (ponceau) for the three experimental replicates.

		Exp1_2_3_timecourse.zip: Zip file containing the ImageStudio file for the western blot.
		
		Quantification: excel file with the quantification from the WB.
	
	PCR: RT-PCR file
		EXP1_quantification: Raw data for the PCR for exp1, analysed using the DeltaDelta Ct method. Geometric mean of 4 housekeeping gene was used (GAPDH, ACTINB, RPL13A2, TBP).
		EXP2_quantification: Raw data for the PCR for exp2, analysed using the DeltaDelta Ct method. Geometric mean of 4 housekeeping gene was used (GAPDH, ACTINB, RPL13A2, TBP).
		EXP3_quantification: Raw data for the PCR for exp3, analysed using the DeltaDelta Ct method. Geometric mean of 4 housekeeping gene was used (GAPDH, ACTINB, RPL13A2, TBP).
		Analysis:  File combaining the 3 experiment to caluclate the 2^-ddCt for all the target genes.
	
	PPM1H_timecourse:  Prism file containing the statistical analysis and graphs for both WB and PCR quantification. Ordinary one-way ANOVA with Dunnett’s comparison test

DRB_CHX folder
In this folder there are the raw files for the DRB and CHX experiment (figure 4D, E, F). PPM1H WT and KO MEFs were treated with or withou O/A in combination with DMSO, DRB or CHX
	WB: Western blot files
		PNG files:
		Blot1: red channel from licor blot showing Western blot for PPM1H WT and KO MEFs. tot Ub, OPA1, and tot Rab10 are in this channel
		Blot1_1: green channel from licor blot showing Western blot for PPM1H WT and KO MEFs. pSer65 Ub, totRab8a, pSer2 RNA pol, LAMP1, PPM1H and pRab10  are in this channel.
		Blot2: Gapdh (red) Western blot for PPM1H WT and KO MEFs.
		DRB_CHX_exp1_2_3 : panel showing the three different experimental replicates

		Exp1_2_3_DRB_CHX: Zip file containing the ImageStudio file for the western blot.
		
		Quantification_WB: excel file with the quantification from the WB.
	
	PCR: RT-PCR file
		EXP1_quantification: Raw data for the PCR for exp1, analysed using the DeltaDelta Ct method. Geometric mean of 4 housekeeping gene was used (GAPDH, ACTINB, RPL13A2, TBP).
		EXP2_quantification: Raw data for the PCR for exp2, analysed using the DeltaDelta Ct method. Geometric mean of 4 housekeeping gene was used (GAPDH, ACTINB, RPL13A2, TBP).
		EXP3_quantification: Raw data for the PCR for exp3, analysed using the DeltaDelta Ct method. Geometric mean of 4 housekeeping gene was used (GAPDH, ACTINB, RPL13A2, TBP).
		Analysis:  File combaining the 3 experiment to caluclate the 2^-ddCt for all the target genes.
	
	DRB_experiments:  Prism file containing the statistical analysis and graphs for both WB and PCR quantification. Ordinary two-way ANOVA with Tukey’s multiple comparison test.

Mitochondria folder
	Mitochondrial fraction
	In this folder there are the blots for mitochondrial fraction from PPM1H WT and PPM1H KO MEFs.
		PNG files:
		Blot1_red: red channel from licor blot showing Western blot for PPM1H WT and PPM1H KO MEFs. Tot Ub, tot LRRk2, OPA1, tot Rab10 are in this channel.
		Blot1_green: green channel from licor blot showing Western blot for PPM1H WT and PPM1H KO MEFs. pUb, pLRRk2, Lamp1, pRab10, and TOM20 are in this channel.
	
		Blot2_red: red channel from licor blot showing Western blot for PPM1H WT and PPM1H KO MEFs. Tubulin, gapdh and tot Rab12 are in this channel.
		Blot2_green: green channel from licor blot showing Western blot for PPM1H WT and PPM1H KO MEFs. Vinculin, HSP60, LC3 and pRab12 are in this channel

		Blot3_red: red channel from licor blot showing Western blot for PPM1H WT and PPM1H KO MEFs. Tom40 is in this channel.
		Blot2_green: green channel from licor blot showing Western blot for PPM1H WT and PPM1H KO MEFs. Calnexin, CS and Cisd1  are in this channel

		Mitofraction_PPm1H_wt_KO: Zip file containing the Imagestudio raw file for the Western blots.

	IF images
	Images from MEF with PPM1H-mAPPLE and mito-GFP treated with or withou oligomycin/antimycin and in normal or hypotonic medium.