Chance, Rachel J.Cameron, Gary A.Fordyce, MarkNoguera, PatriciaWang, TiehuiCollins, CatherineSecombes, Christopher J.Collet, Bertrand2018FIGURE 5 in Effects of repeated anaesthesia on gill and general health of Atlantic salmon, Salmo salar10.5281/zenodo.10928788FIGURE 5 Mean (+SE) relative expression of selected genes in Salmo salar gills. Gene expression level was quantified by real-time PCR, normalized using the housekeeping gene elongation factor α (elfα), and expressed as arbitrary unit where the expression in single exposure is 1. Gills were analysed after a single (n = 10) or repeated exposure (n = 30) to (a) MS-222 (80 mg l−1) () Single, and () Repeat, (b) metomidate (12.5 mg l−1) () Single, and () Repeat and (c) AQUI-S (17 mg l−1) () Single, and () Repeat. Log -transformed data were 10 compared by one-way ANOVA. *P <0.05; **, P <0.01; ***P <0.001***************