Differential regulation by CD47 and thrombospondin-1 of extramedullary erythropoiesis in mouse spleen
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1.
National Cancer Institute
Description
Datasets corresponding to eLife publication: Differential regulation by CD47 and thrombospondin-1 of extramedullary erythropoiesis in mouse spleen
Rajdeep Banerjee1, Thomas J. Meyer2, Margaret C. Cam2, Sukhbir Kaur1 and David D. Roberts1,
1Laboratory of Pathology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA
2CCR Collaborative Bioinformatics Resource, Office of Science and Technology Resources, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA
biorXiv preprint 2023 Sep 28:2023.09.28.559992 doi: https://doi.org/10.1101/2023.09.28.559992
eLife pre-review https://doi.org/10.7554/eLife.92679.1
Flow cytometry data for Figure 1G-R: Effects of Cd47 or Thbs1 gene disruption on spleen cell numbers and content of cells expressing erythropoietic precursor markers or the proliferation marker Ki67.
Files:
elife92679.1 Fig 1G-R CD47KO sample-1-Aqp1.fcs
elife92679.1 Fig 1G-R CD47KO sample-1- Epor.fcs
elife92679.1 Fig 1G-R CD47KO sample-1-Ermap.fcs
elife92679.1 Fig 1G-R CD47KO sample-1-Gypa.fcs
elife92679.1 Fig 1G-R CD47KO sample-2-Aqp1.fcs
elife92679.1 Fig 1G-R CD47KO sample-2-Epor.fcs
elife92679.1 Fig 1G-R CD47KO sample-2-Ermap.fcs
elife92679.1 Fig 1G-R CD47KO sample-2-Gypa.fcs
elife92679.1 Fig 1G-R CD47KO sample-3-Aqp1 .fcs
elife92679.1 Fig 1G-R CD47KO sample-3- Epor.fcs
elife92679.1 Fig 1G-R CD47KO sample-3-Ermap.fcs
elife92679.1 Fig 1G-R CD47KO sample-3-Gypa.fcs
elife92679.1 Fig 1 G-R TSP1 KO sample-1-Aqp1.fcs
elife92679.1 Fig 1 G-R TSP1 KO sample-1-Epor.fcs
eLife92679.1 Fig 1G-R TSP1KO sample-1-Ermap.fcs
elife92679.1 Fig 1G-R TSP1KO sample-1-Gypa.fcs
elife92679.1 Fig 1 G-R TSP1 KO sample-2-Aqp1.fcs
elife92679.1 Fig 1G-R TSP1 KO sample-2-Epor.fcs
elife92679.1 Fig 1G-R TSP1KO sample-2-Ermap.fcs
elife92679.1 Fig 1G-R TSP1 KO sample-2-Gypa.fcs
elife92679.1 Fig 1G-R TSP1 KO sample-3-Aqp1.fcs
elife92679.1 Fig 1G-R TSP1 KO sample-3-Epor.fcs
elife92679.1 Fig 1G-R TSP1 KO sample-3-Ermap.fcs
elife92679.1 Fig 1G-R TSP1 KO sample-3-Gypa.fcs
elife92679.1 Fig 1G-R WT sample-1-Aqp1.fcs
eufe92679.1 Fig 1G-R WT sample-1-Epor.fcs
elife92679.1 Fig 1 G-R WT sample-1-Ermap.fcs
elife92679.1 Fig 1 G-R WT sample-1-Gypa.fcs
elife92679.1 Fig 1G-R WT sample-2-Aqp1 .fcs
elife92679.1 Fig 1G-R WT sample-2-Epor.fcs
elife92679.1 Fig 1G-R WT sample-2-Ermap.fcs
elife92679.1 Fig 1G-R WT sample-2-Gypa.fcs
elife92679.1 Fig 1G-R WT sample-3-Aqp1 .fcs
elife92679.1 Fig 1G-R WT sample-3-Epor.fcs
elife92679.1 Fig 1G-R WT sample-3-Ermap.fcs
elife92679.1 Fig 1G-R WT sample-3-Gypa.fcs
elife92679.1 2ndary cont.fcs secondary antibody control
Figure 1B-F and figure 2: Effects of Cd47 or Thbs1 gene disruption on the percentage of Ter119+ and Ter119− spleen cells expressing markers of multipotent and committed erythroid precursors and cell proliferation.
Files:
elife92679.1 Fig 2 CD47KO sample- 1.fcs
elife92679.1 Fig 2 CD47KO sample-2.fcs
elife92679.1 Fig 2 CD47KO sample-3.fcs
elife92679.1 Fig 2 CD47KO sample-4.fcs
elife92679.1 Fig 2 TSP1KO sample-1.fcs
elife92679.1 Fig 2 TSP1 KO sample-2.fcs
elife92679.1 Fig 2 TSP1 KO sample-3.fcs
elife92679.1 Fig 2 TSP1 KO sample-4.fcs
elife92679.1 Fig 2 WT sample-1.fcs
elife92679.1 Fig 2 WT sample-2.fcs
elife92679.1 Fig 2 WT sample-3.fcs
elife92679.1 Fig 2 WT sample-4.fcs
elife92679.1 Ter119 cont.fcs isotype control
elife92679.1 Sca1 cont.fcs isotype control
elife92679.1 Ki67 cont.fcs isotype control
elife92679.1 cKit cont.fcs isotype control
elife92679.1 CD34 cont.fcs isotype control
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Additional details
Funding
- Cellular Interactions with Thrombospondin ZIA SC 009172
- National Cancer Institute