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Published April 1, 2024 | Version v1
Journal article Open

Differential regulation by CD47 and thrombospondin-1 of extramedullary erythropoiesis in mouse spleen

  • 1. ROR icon National Cancer Institute

Description

Datasets corresponding to eLife publication: Differential regulation by CD47 and thrombospondin-1 of extramedullary erythropoiesis in mouse spleen 

Rajdeep Banerjee1, Thomas J. Meyer2, Margaret C. Cam2, Sukhbir Kaur1 and David D. Roberts1, 

1Laboratory of Pathology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA

2CCR Collaborative Bioinformatics Resource, Office of Science and Technology Resources, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA

biorXiv preprint 2023 Sep 28:2023.09.28.559992 doi: https://doi.org/10.1101/2023.09.28.559992

eLife pre-review https://doi.org/10.7554/eLife.92679.1

Flow cytometry data for Figure 1G-R: Effects of Cd47 or Thbs1 gene disruption on spleen cell numbers and content of cells expressing erythropoietic precursor markers or the proliferation marker Ki67.

Files:

elife92679.1 Fig 1G-R CD47KO sample-1-Aqp1.fcs

elife92679.1 Fig 1G-R CD47KO sample-1- Epor.fcs

elife92679.1 Fig 1G-R CD47KO sample-1-Ermap.fcs

elife92679.1 Fig 1G-R CD47KO sample-1-Gypa.fcs

elife92679.1 Fig 1G-R CD47KO sample-2-Aqp1.fcs

elife92679.1 Fig 1G-R CD47KO sample-2-Epor.fcs

elife92679.1 Fig 1G-R CD47KO sample-2-Ermap.fcs

elife92679.1 Fig 1G-R CD47KO sample-2-Gypa.fcs

elife92679.1 Fig 1G-R CD47KO sample-3-Aqp1 .fcs

elife92679.1 Fig 1G-R CD47KO sample-3- Epor.fcs

elife92679.1 Fig 1G-R CD47KO sample-3-Ermap.fcs

elife92679.1 Fig 1G-R CD47KO sample-3-Gypa.fcs

elife92679.1 Fig 1 G-R TSP1 KO sample-1-Aqp1.fcs

elife92679.1 Fig 1 G-R TSP1 KO sample-1-Epor.fcs

eLife92679.1 Fig 1G-R TSP1KO sample-1-Ermap.fcs

elife92679.1 Fig 1G-R TSP1KO sample-1-Gypa.fcs

elife92679.1 Fig 1 G-R TSP1 KO sample-2-Aqp1.fcs

elife92679.1 Fig 1G-R TSP1 KO sample-2-Epor.fcs

elife92679.1 Fig 1G-R TSP1KO sample-2-Ermap.fcs

elife92679.1 Fig 1G-R TSP1 KO sample-2-Gypa.fcs

elife92679.1 Fig 1G-R TSP1 KO sample-3-Aqp1.fcs

elife92679.1 Fig 1G-R TSP1 KO sample-3-Epor.fcs

elife92679.1 Fig 1G-R TSP1 KO sample-3-Ermap.fcs

elife92679.1 Fig 1G-R TSP1 KO sample-3-Gypa.fcs

elife92679.1 Fig 1G-R WT sample-1-Aqp1.fcs

eufe92679.1 Fig 1G-R WT sample-1-Epor.fcs

elife92679.1 Fig 1 G-R WT sample-1-Ermap.fcs

elife92679.1 Fig 1 G-R WT sample-1-Gypa.fcs

elife92679.1 Fig 1G-R WT sample-2-Aqp1 .fcs

elife92679.1 Fig 1G-R WT sample-2-Epor.fcs

elife92679.1 Fig 1G-R WT sample-2-Ermap.fcs

elife92679.1 Fig 1G-R WT sample-2-Gypa.fcs  

elife92679.1 Fig 1G-R WT sample-3-Aqp1 .fcs

elife92679.1 Fig 1G-R WT sample-3-Epor.fcs

elife92679.1 Fig 1G-R WT sample-3-Ermap.fcs

elife92679.1 Fig 1G-R WT sample-3-Gypa.fcs

elife92679.1 2ndary cont.fcs       secondary antibody control

Figure 1B-F and figure 2: Effects of Cd47 or Thbs1 gene disruption on the percentage of Ter119+ and Ter119spleen cells expressing markers of multipotent and committed erythroid precursors and cell proliferation.

Files:

elife92679.1 Fig 2 CD47KO sample- 1.fcs

elife92679.1 Fig 2 CD47KO sample-2.fcs  

elife92679.1 Fig 2 CD47KO sample-3.fcs  

elife92679.1 Fig 2 CD47KO sample-4.fcs  

elife92679.1 Fig 2 TSP1KO sample-1.fcs  

elife92679.1 Fig 2 TSP1 KO sample-2.fcs

elife92679.1 Fig 2 TSP1 KO sample-3.fcs  

elife92679.1 Fig 2 TSP1 KO sample-4.fcs  

elife92679.1 Fig 2 WT sample-1.fcs

elife92679.1 Fig 2 WT sample-2.fcs

elife92679.1 Fig 2 WT sample-3.fcs  

elife92679.1 Fig 2 WT sample-4.fcs  

elife92679.1 Ter119 cont.fcs   isotype control

elife92679.1 Sca1 cont.fcs      isotype control

elife92679.1 Ki67 cont.fcs       isotype control

elife92679.1 cKit cont.fcs       isotype control

elife92679.1 CD34 cont.fcs     isotype control

Files

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Additional details

Funding

Cellular Interactions with Thrombospondin ZIA SC 009172
National Cancer Institute