File	Item	General_condition	Specific_condition / Notes	Experiment
				
Characterization of substrates and intermediates				
AfG3PS_NMR_1	Purification of 1b	ca. 2 mg compound E-1b, CDCl3, 25 °C		1H NMR
AfG3PS_NMR_2	Synthesis of 1	ca. 4 mg compound E-1, CD3CN, 25 °C		1H NMR
AfG3PS_NMR_3	Synthesis of 1	ca. 4 mg compound E-1, CD3CN, 25 °C		31P NMR
AfG3PS_NMR_4	Synthesis of 1	ca. 4 mg compound E-1, CD3CN, 25 °C		13C NMR
AfG3PS_NMR_5	Synthesis of 1	ca. 4 mg compound E-1, CD3CN, 25 °C		13C-Dept135
AfG3PS_NMR_6	Synthesis of 2	ca. 4 mg compound 15:1 E/Z-2, CD3CN, 25 °C		1H NMR
AfG3PS_NMR_7	Synthesis of 2	ca. 4 mg compound 15:1 E/Z-2, CD3CN, 25 °C		31P NMR
AfG3PS_NMR_8	Synthesis of 2	ca. 4 mg compound 15:1 E/Z-2, CD3CN, 25 °C		13C NMR
AfG3PS_NMR_9	Synthesis of 2	ca. 4 mg compound 15:1 E/Z-2, CD3CN, 25 °C		13C-Dept135
AfG3PS_NMR_10	Synthesis of E-3	ca. 4 mg compound 6.6:1 E/Z-3, CD3CN, 25 °C		1H NMR
AfG3PS_NMR_11	Synthesis of E-3	ca. 4 mg compound 6.6:1 E/Z-3, CD3CN, 25 °C		31P NMR
AfG3PS_NMR_12	Synthesis of E-3	ca. 4 mg compound 6.6:1 E/Z-3, CD3CN, 25 °C		13C NMR
AfG3PS_NMR_13	Synthesis of E-3	ca. 4 mg compound 6.6:1 E/Z-3, CD3CN, 25 °C		13C-Dept135
AfG3PS_NMR_14	Reference of Z-3b	ca. 2 mg compound Z-3b, CDCl3, 0.05% TMS, 25 °C		1H NMR
AfG3PS_NMR_15	Synthesis of Z-3	ca. 4 mg compound 1:4.4 E/Z-3, D2O, 0.05% TSP, 25 °C		1H NMR
AfG3PS_NMR_16	Synthesis of Z-3	ca. 4 mg compound 1:4.4 E/Z-3, D2O, 0.05% TSP, 25 °C		31P NMR
AfG3PS_NMR_17	Synthesis of Z-3	ca. 4 mg compound 1:4.4 E/Z-3, D2O, 0.05% TSP, 25 °C		13C NMR
AfG3PS_NMR_18	Synthesis of Z-3	ca. 4 mg compound 1:4.4 E/Z-3, D2O, 0.05% TSP, 25 °C		13C-Dept135
AfG3PS_NMR_19	Synthesis of 4	ca. 4 mg compound 4, CD3CN, 0.05% TMS, 25 °C		1H NMR
AfG3PS_NMR_20	Synthesis of 4	ca. 4 mg compound 4, CD3CN, 0.05% TMS, 25 °C		31P NMR
AfG3PS_NMR_21	Synthesis of 4	ca. 4 mg compound 4, CD3CN, 0.05% TMS, 25 °C		13C NMR
AfG3PS_NMR_22	Synthesis of 4	ca. 4 mg compound 4, CD3CN, 0.05% TMS, 25 °C		13C-Dept135
AfG3PS_NMR_23	Synthesis of 5a	ca. 2 mg compound 2.3:1 E/Z-5a, CDCl3, 25 °C	contains residual EtOAc as an impurity	1H NMR
AfG3PS_NMR_24	Synthesis of 5	ca. 4 mg compound 2.3:1 E/Z-5, CD3CN, 25 °C	contains residual iPrOH as a minor impurity	1H NMR
AfG3PS_NMR_25	Synthesis of 5	ca. 4 mg compound 2.3:1 E/Z-5, CD3CN, 25 °C	contains residual iPrOH as a minor impurity	31P NMR
AfG3PS_NMR_26	Synthesis of 5	ca. 4 mg compound 2.3:1 E/Z-5, CD3CN, 25 °C	contains residual iPrOH as a minor impurity	13C NMR
AfG3PS_NMR_27	Synthesis of 5	ca. 4 mg compound 2.3:1 E/Z-5, CD3CN, 25 °C	contains residual iPrOH as a minor impurity	13C-Dept135
AfG3PS_NMR_28	Synthesis of 6c	ca. 10 mg compound 6c, CDCl3, 25 °C		1H NMR
AfG3PS_NMR_29	Synthesis of 6c	ca. 10 mg compound 6c, CDCl3, 25 °C		13C NMR
AfG3PS_NMR_30	Synthesis of 6b	ca. 10 mg compound 3.4:1 E/Z-6b, wet DMSO-d6, 25 °C	contains H2O	1H NMR
AfG3PS_NMR_31	Synthesis of 6b	ca. 10 mg compound 3.4:1 E/Z-6b, wet DMSO-d6, 25 °C	contains H2O	13C NMR
AfG3PS_NMR_32	Synthesis of 6	ca. 4 mg compound 2.2:1 E/Z-6, CD3CN, 0.05% TMS, 25 °C	contains the monophosphate as a minor impurity	1H NMR
AfG3PS_NMR_33	Synthesis of 6	ca. 4 mg compound 2.2:1 E/Z-6, CD3CN, 0.05% TMS, 25 °C	contains the monophosphate as a minor impurity	31P NMR
AfG3PS_NMR_34	Synthesis of 6	ca. 4 mg compound 2.2:1 E/Z-6, CD3CN, 0.05% TMS, 25 °C	contains the monophosphate as a minor impurity	13C NMR
AfG3PS_NMR_35	Synthesis of 6	ca. 4 mg compound 2.2:1 E/Z-6, CD3CN, 0.05% TMS, 25 °C	contains the monophosphate as a minor impurity	13C-Dept135
AfG3PS_NMR_36	Synthesis of 7a	ca. 2 mg compound 7a, CDCl3, 25 °C	contains residual cyclohexane as an impurity	1H NMR
AfG3PS_NMR_37	Synthesis of 7	ca. 4 mg compound 7, CD3CN, 25 °C		1H NMR
AfG3PS_NMR_38	Synthesis of 7	ca. 4 mg compound 7, CD3CN, 25 °C		31P NMR
AfG3PS_NMR_39	Synthesis of 7	ca. 4 mg compound 7, CD3CN, 25 °C		13C NMR
AfG3PS_NMR_40	Synthesis of 7	ca. 4 mg compound 7, CD3CN, 25 °C		13C-Dept135
AfG3PS_NMR_41	Synthesis of 8	ca. 4 mg compound 8, CD3CN, 0.05% TMS, 25 °C		1H NMR
AfG3PS_NMR_42	Synthesis of 8	ca. 4 mg compound 8, CD3CN, 0.05% TMS, 25 °C		31P NMR
AfG3PS_NMR_43	Synthesis of 8	ca. 4 mg compound 8, CD3CN, 0.05% TMS, 25 °C		13C NMR
AfG3PS_NMR_44	Synthesis of 8	ca. 4 mg compound 8, CD3CN, 0.05% TMS, 25 °C		13C-Dept135
AfG3PS_NMR_45	Synthesis of 9b	ca. 2 mg compound E-9b, CDCl3, 25 °C	contains H2O as a minor impurity	1H NMR
AfG3PS_NMR_46	Synthesis of 9	ca. 4 mg compound E-9, CD3CN, 0.05% TMS, 25 °C	contains CDCl3, H2O and pyrophosphate as impurities	1H NMR
AfG3PS_NMR_47	Synthesis of 9	ca. 4 mg compound E-9, CD3CN, 0.05% TMS, 25 °C	contains CDCl3, H2O and pyrophosphate as impurities	31P NMR
AfG3PS_NMR_48	Synthesis of 9	ca. 4 mg compound E-9, CD3CN, 0.05% TMS, 25 °C	contains CDCl3, H2O and pyrophosphate as impurities	13C NMR
AfG3PS_NMR_49	Synthesis of 9	ca. 4 mg compound E-9, CD3CN, 0.05% TMS, 25 °C	contains CDCl3, H2O and pyrophosphate as impurities	13C-Dept135
AfG3PS_NMR_50	Synthesis of 10	ca. 4 mg compound E-10, CD3CN, 0.05% TMS, 25 °C		1H NMR
AfG3PS_NMR_51	Synthesis of 10	ca. 4 mg compound E-10, CD3CN, 0.05% TMS, 25 °C		31P NMR
AfG3PS_NMR_52	Synthesis of 10	ca. 4 mg compound E-10, CD3CN, 0.05% TMS, 25 °C		13C NMR
AfG3PS_NMR_53	Synthesis of 11	ca. 4 mg compound 11, CD3CN, 25 °C	contains residual iPrOH as an impurity	1H NMR
AfG3PS_NMR_54	Synthesis of 11	ca. 4 mg compound 11, CD3CN, 25 °C	contains residual iPrOH as an impurity	31P NMR
AfG3PS_NMR_55	Synthesis of 11	ca. 4 mg compound 11, CD3CN, 25 °C	contains residual iPrOH as an impurity	13C NMR
AfG3PS_NMR_56	Synthesis of 11	ca. 4 mg compound 11, CD3CN, 25 °C	contains residual iPrOH as an impurity	13C-Dept135
AfG3PS_NMR_57	Synthesis of 12	ca. 4 mg compound 12, CD3CN, 0.05% TMS, 25 °C		1H NMR
AfG3PS_NMR_58	Synthesis of 12	ca. 4 mg compound 12, CD3CN, 0.05% TMS, 25 °C		31P NMR
AfG3PS_NMR_59	Synthesis of 12	ca. 4 mg compound 12, CD3CN, 0.05% TMS, 25 °C		13C NMR
AfG3PS_NMR_60	Synthesis of 12	ca. 4 mg compound 12, CD3CN, 0.05% TMS, 25 °C		13C-Dept135
AfG3PS_NMR_61	Synthesis of 13	ca. 4 mg compound 13, CD3CN, 25 °C	contains residual iPrOH as an impurity	1H NMR
AfG3PS_NMR_62	Synthesis of 13	ca. 4 mg compound 13, CD3CN, 25 °C	contains residual iPrOH as an impurity	31P NMR
AfG3PS_NMR_63	Synthesis of 13	ca. 4 mg compound 13, CD3CN, 25 °C	contains residual iPrOH as an impurity	13C NMR
AfG3PS_NMR_64	Synthesis of 13	ca. 4 mg compound 13, CD3CN, 25 °C	contains residual iPrOH as an impurity	13C-Dept135
AfG3PS_NMR_65	Synthesis of 14	ca. 8 mg compound 14, CD3CN, 0.05% TMS, 25 °C		1H NMR
AfG3PS_NMR_66	Synthesis of 14	ca. 8 mg compound 14, CD3CN, 0.05% TMS, 25 °C		31P NMR
AfG3PS_NMR_67	Synthesis of 14	ca. 8 mg compound 14, CD3CN, 0.05% TMS, 25 °C		13C NMR
AfG3PS_NMR_68	Synthesis of 14	ca. 8 mg compound 14, CD3CN, 0.05% TMS, 25 °C		13C-Dept135
AfG3PS_NMR_69	Synthesis of 15	ca. 6 mg compound 15, CD3CN, 0.05% TMS, 25 °C		1H NMR
AfG3PS_NMR_70	Synthesis of 15	ca. 6 mg compound 15, CD3CN, 0.05% TMS, 25 °C		31P NMR
AfG3PS_NMR_71	Synthesis of 15	ca. 6 mg compound 15, CD3CN, 0.05% TMS, 25 °C		13C NMR
AfG3PS_NMR_72	Synthesis of 15	ca. 6 mg compound 15, CD3CN, 0.05% TMS, 25 °C		13C-Dept135
AfG3PS_NMR_73	Synthesis of 16	ca. 6 mg compound 16, CD3CN, 0.05% TMS, 25 °C	contains EtOAc as a minor impurity	1H NMR
AfG3PS_NMR_74	Synthesis of 16	ca. 6 mg compound 16, CD3CN, 0.05% TMS, 25 °C	contains EtOAc as a minor impurity	31P NMR
AfG3PS_NMR_75	Synthesis of 16	ca. 6 mg compound 16, CD3CN, 0.05% TMS, 25 °C	contains EtOAc as a minor impurity	13C NMR
AfG3PS_NMR_76	Synthesis of 16	ca. 6 mg compound 16, CD3CN, 0.05% TMS, 25 °C	contains EtOAc as a minor impurity	13C-Dept135
AfG3PS_NMR_77	Synthesis of 17	ca. 6 mg compound 17, CD3CN, 0.05% TMS, 25 °C	contains residual CDCl3 as a minor impurity	1H NMR
AfG3PS_NMR_78	Synthesis of 17	ca. 6 mg compound 17, CD3CN, 0.05% TMS, 25 °C	contains residual CDCl3 as a minor impurity	31P NMR
AfG3PS_NMR_79	Synthesis of 17	ca. 6 mg compound 17, CD3CN, 0.05% TMS, 25 °C	contains residual CDCl3 as a minor impurity	13C NMR
AfG3PS_NMR_80	Synthesis of 17	ca. 6 mg compound 17, CD3CN, 0.05% TMS, 25 °C	contains residual CDCl3 as a minor impurity	13C-Dept135
AfG3PS_NMR_81	Synthesis of 18c	ca. 10 mg compound 18c, CDCl3, 25 °C	contains an unidentified impurity	1H NMR
AfG3PS_NMR_82	Synthesis of 18c	ca. 10 mg compound 18c, CDCl3, 25 °C	contains an unidentified impurity	13C NMR
AfG3PS_NMR_83	Synthesis of 18b	ca. 8 mg compound 18b, CDCl3, 25 °C	contains an unidentified impurity	1H NMR
AfG3PS_NMR_84	Synthesis of 18	ca. 4 mg compound 18, CD3CN, 0.05% TMS, 25 °C		1H NMR
AfG3PS_NMR_85	Synthesis of 18	ca. 4 mg compound 18, CD3CN, 0.05% TMS, 25 °C		31P NMR
AfG3PS_NMR_86	Synthesis of 18	ca. 4 mg compound 18, CD3CN, 0.05% TMS, 25 °C		13C NMR
AfG3PS_NMR_87	Synthesis of 18	ca. 4 mg compound 18, CD3CN, 0.05% TMS, 25 °C		13C-Dept135
AfG3PS_NMR_88	Synthesis of 18	ca. 4 mg compound 18, D2O, 0.05% TSP, 25 °C	contains pyrophosphate as an impurity and minor amounts of degradation products	1H NMR
AfG3PS_NMR_89	Synthesis of 18	ca. 4 mg compound 18, D2O, 0.05% TSP, 25 °C	contains pyrophosphate as an impurity and minor amounts of degradation products	31P NMR
AfG3PS_NMR_90	Synthesis of 19c	ca. 10 mg compound 19c, CDCl3, 25 °C	contains an unidentified impurity	1H NMR
AfG3PS_NMR_91	Synthesis of 19c	ca. 10 mg compound 19c, CDCl3, 25 °C	contains an unidentified impurity	13C NMR
AfG3PS_NMR_92	Synthesis of 19b	ca. 8 mg compound 19b, CDCl3, 25 °C	contains an unidentified impurity	1H NMR
AfG3PS_NMR_93	Synthesis of 19	ca. 4 mg compound 19, CD3CN, 0.05% TMS, 25 °C		1H NMR
AfG3PS_NMR_94	Synthesis of 19	ca. 4 mg compound 19, CD3CN, 0.05% TMS, 25 °C		31P NMR
AfG3PS_NMR_95	Synthesis of 19	ca. 4 mg compound 19, CD3CN, 0.05% TMS, 25 °C		13C NMR
AfG3PS_NMR_96	Synthesis of 19	ca. 4 mg compound 19, CD3CN, 0.05% TMS, 25 °C		13C-Dept135
AfG3PS_NMR_97	Synthesis of 20c	ca. 12 mg compound 20c, CDCl3, 0.05% TMS, 25 °C	contains an unidentified impurity	1H NMR
AfG3PS_NMR_98	Synthesis of 20c	ca. 12 mg compound 20c, CDCl3, 0.05% TMS, 25 °C	contains an unidentified impurity	13C NMR
AfG3PS_NMR_99	Synthesis of 20c	ca. 12 mg compound 20c, CDCl3, 0.05% TMS, 25 °C	contains an unidentified impurity	13C-Dept135
AfG3PS_NMR_100	Synthesis of 20b	ca. 8 mg compound 20b, CDCl3, 0.05% TMS, 25 °C	crude material, contains minor impurities	1H NMR
AfG3PS_NMR_101	Synthesis of 20	ca. 4 mg compound 20, CD3CN, 0.05% TMS, 25 °C		1H NMR
AfG3PS_NMR_102	Synthesis of 20	ca. 4 mg compound 20, CD3CN, 0.05% TMS, 25 °C		31P NMR
AfG3PS_NMR_103	Synthesis of 20	ca. 4 mg compound 20, CD3CN, 0.05% TMS, 25 °C		13C NMR
AfG3PS_NMR_104	Synthesis of 20	ca. 4 mg compound 20, CD3CN, 0.05% TMS, 25 °C		13C-Dept135
AfG3PS_NMR_105	Synthesis of 20	ca. 4 mg compound 20, D2O, 0.05% TSP, 25 °C	contains minor amounts of degradation products	1H NMR
AfG3PS_NMR_106	Synthesis of 20	ca. 4 mg compound 20, D2O, 0.05% TSP, 25 °C	contains minor amounts of degradation products	31P NMR
AfG3PS_NMR_107	Synthesis of 21c	ca. 12 mg compound 21c, CDCl3, 0.05% TMS, 25 °C		1H NMR
AfG3PS_NMR_108	Synthesis of 21c	ca. 12 mg compound 21c, CDCl3, 0.05% TMS, 25 °C		13C NMR
AfG3PS_NMR_109	Synthesis of 21c	ca. 12 mg compound 21c, CDCl3, 0.05% TMS, 25 °C		13C-Dept135
AfG3PS_NMR_110	Synthesis of 21c	ca. 10 mg compound 21b, CDCl3, 0.05% TMS, 25 °C	contains an unidentified impurity	1H NMR
AfG3PS_NMR_111	Synthesis of 21c	ca. 10 mg compound 21b, CDCl3, 0.05% TMS, 25 °C	contains an unidentified impurity	13C NMR
AfG3PS_NMR_112	Synthesis of 21c	ca. 10 mg compound 21b, CDCl3, 0.05% TMS, 25 °C	contains an unidentified impurity	13C-Dept135
AfG3PS_NMR_113	Synthesis of 21	ca. 4 mg compound 21, CD3CN, 0.05% TMS, 25 °C	contains 20 as an impurity	1H NMR
AfG3PS_NMR_114	Synthesis of 21	ca. 4 mg compound 21, CD3CN, 0.05% TMS, 25 °C	contains 20 as an impurity	31P NMR
AfG3PS_NMR_115	Synthesis of 21	ca. 4 mg compound 21, CD3CN, 0.05% TMS, 25 °C	contains 20 as an impurity	13C NMR
AfG3PS_NMR_116	Synthesis of 21	ca. 4 mg compound 21, CD3CN, 0.05% TMS, 25 °C	contains 20 as an impurity	13C-Dept135
AfG3PS_NMR_117	Synthesis of 21	ca. 4 mg compound 21, D2O, 0.05% TSP, 25 °C	contains 20 and minor amounts of degradation products	1H NMR
AfG3PS_NMR_118	Synthesis of 21	ca. 4 mg compound 21, D2O, 0.05% TSP, 25 °C	contains 20 and minor amounts of degradation products	31P NMR
AfG3PS_NMR_119	Synthesis of 2-deoxy-GP	ca. 8 mg compound 2-deoxy-GP, D2O, 0.05% TSP, 25 °C	also contains compound S2	1H NMR
AfG3PS_NMR_120	Synthesis of 2-deoxy-GP	ca. 8 mg compound 2-deoxy-GP, D2O, 0.05% TSP, 25 °C	also contains compound S2	31P NMR
AfG3PS_NMR_121	Synthesis of 2-deoxy-GP	ca. 8 mg compound 2-deoxy-GP, D2O, 0.05% TSP, 25 °C	also contains compound S2	13C NMR
AfG3PS_NMR_122	Synthesis of 2-deoxy-GP	ca. 8 mg compound 2-deoxy-GP, D2O, 0.05% TSP, 25 °C	also contains compound S2	13C-Dept135
				
Characterization of product standards				
AfG3PS_NMR_123	Synthesis of rac-2G	ca. 2 mg compound rac-2G, CDCl3, 0.05% TMS, 25 °C		1H NMR
AfG3PS_NMR_124	Synthesis of rac-2G	ca. 2 mg compound rac-2G, CDCl3, 0.05% TMS, 25 °C		13C NMR
AfG3PS_NMR_125	Synthesis of rac-2G	ca. 2 mg compound rac-2G, CDCl3, 0.05% TMS, 25 °C		13C-Dept135
AfG3PS_NMR_126	Synthesis of R-2G	ca. 2 mg compound R-2G, CDCl3, 0.05% TMS, 25 °C	contains residual EtOAc as a minor impurity	1H NMR
AfG3PS_NMR_127	Synthesis of rac-3G	ca. 2 mg compound rac-3G, CDCl3, 0.05% TMS, 25 °C	contains residual EtOAc as a minor impurity	1H NMR
AfG3PS_NMR_128	Synthesis of rac-3G	ca. 2 mg compound rac-3G, CDCl3, 0.05% TMS, 25 °C	contains residual EtOAc as a minor impurity	13C NMR
AfG3PS_NMR_129	Synthesis of rac-3G	ca. 2 mg compound rac-3G, CDCl3, 0.05% TMS, 25 °C	contains residual EtOAc as a minor impurity	13C-Dept135
AfG3PS_NMR_130	Synthesis of 5G, Figure 2a	ca. 2 mg compound rac-5G, CDCl3, 0.05% TMS, 25 °C	contains minor unidentified impurities	1H NMR
AfG3PS_NMR_131	Synthesis of 5G	ca. 2 mg compound rac-5G, CDCl3, 0.05% TMS, 25 °C	contains minor unidentified impurities	13C NMR
AfG3PS_NMR_132	Synthesis of 5G	ca. 2 mg compound rac-5G, CDCl3, 0.05% TMS, 25 °C	contains minor unidentified impurities	13C-Dept135
				
Characterization of enzymatic products				
AfG3PS_NMR_133	Biocatalytic synthesis of 2G, Figure 2a	enzymatic product from 2, CDCl3, 0.05% TMS, 25 °C	replicate 1, contains minor unidentified impurities	1H NMR
AfG3PS_NMR_134	Biocatalytic synthesis of 2G	enzymatic product from 2, CDCl3, 0.05% TMS, 25 °C	replicate 2, contains minor unidentified impurities	1H NMR
AfG3PS_NMR_135	Biocatalytic synthesis of E-3G	enzymatic product from E-3, CDCl3, 0.05% TMS, 25 °C	contains minor unidentified impurities	1H NMR (400 MHz)
AfG3PS_NMR_136	Biocatalytic synthesis of E-3G	enzymatic product from E-3, CDCl3, 0.05% TMS, 25 °C	contains minor unidentified impurities	1H NMR (700 MHz)
AfG3PS_NMR_137	Biocatalytic synthesis of E-3G	enzymatic product from E-3, CDCl3, 0.05% TMS, 25 °C	contains minor unidentified impurities	1H,1H COSY
AfG3PS_NMR_138	Biocatalytic synthesis of E-3G	enzymatic product from E-3, CDCl3, 0.05% TMS, 25 °C	contains minor unidentified impurities	1H,1H NOESY
AfG3PS_NMR_139	Biocatalytic synthesis of Z-3G, Figure 2a	enzymatic product from Z-3, CDCl3, 0.05% TMS, 25 °C	contains minor unidentified impurities	1H NMR (400 MHz)
AfG3PS_NMR_140	Biocatalytic synthesis of Z-3G	enzymatic product from Z-3, CDCl3, 0.05% TMS, 25 °C	contains minor unidentified impurities	1H NMR (700 MHz)
AfG3PS_NMR_141	Biocatalytic synthesis of Z-3G	enzymatic product from Z-3, CDCl3, 0.05% TMS, 25 °C	contains minor unidentified impurities	1H,1H COSY
AfG3PS_NMR_142	Biocatalytic synthesis of Z-3G	enzymatic product from Z-3, CDCl3, 0.05% TMS, 25 °C	contains minor unidentified impurities	1H,1H NOESY
AfG3PS_NMR_143	Biocatalytic synthesis of 6G, Figure 2a	enzymatic product from 6, CDCl3, 0.05% TMS, 25 °C	contains minor unidentified impurities	1H NMR (400 MHz)
AfG3PS_NMR_144	Biocatalytic synthesis of 6G	enzymatic product from 6, CDCl3, 0.05% TMS, 25 °C		1H NMR (700 MHz)
AfG3PS_NMR_145	Biocatalytic synthesis of 6G	enzymatic product from 6, CDCl3, 0.05% TMS, 25 °C		1H,1H COSY
AfG3PS_NMR_146	Biocatalytic synthesis of 6G	enzymatic product from 6, CDCl3, 0.05% TMS, 25 °C		1H,1H NOESY
AfG3PS_NMR_147	Biocatalytic synthesis of 10G	enzymatic product from 10, CDCl3, 0.05% TMS, 25 °C	contains residual H2O	1H NMR
AfG3PS_NMR_148	Biocatalytic synthesis of 18G	enzymatic product from 18, CDCl3, 0.05% TMS, 25 °C	contains unidentified impurities	1H NMR (400 MHz)
AfG3PS_NMR_149	Biocatalytic synthesis of 18G	enzymatic product from 18, CDCl3, 0.05% TMS, 25 °C	contains unidentified impurities	1H NMR (700 MHz)
AfG3PS_NMR_150	Biocatalytic synthesis of 18G	enzymatic product from 18, CDCl3, 0.05% TMS, 25 °C	contains unidentified impurities	1H,1H COSY
AfG3PS_NMR_151	Biocatalytic synthesis of 18G	enzymatic product from 18, CDCl3, 0.05% TMS, 25 °C	contains unidentified impurities	1H,1H NOESY
AfG3PS_NMR_152	Biocatalytic synthesis of 19G, Figure 2a	enzymatic product from 19, CDCl3, 0.05% TMS, 25 °C	contains minor unidentified impurities	1H NMR (400 MHz)
AfG3PS_NMR_153	Biocatalytic synthesis of 19G	enzymatic product from 19, CDCl3, 0.05% TMS, 25 °C	contains minor unidentified impurities	1H NMR (700 MHz)
AfG3PS_NMR_154	Biocatalytic synthesis of 19G	enzymatic product from 19, CDCl3, 0.05% TMS, 25 °C	contains minor unidentified impurities	1H,1H COSY
AfG3PS_NMR_155	Biocatalytic synthesis of 19G	enzymatic product from 19, CDCl3, 0.05% TMS, 25 °C	contains minor unidentified impurities	1H,1H NOESY
AfG3PS_NMR_156	Biocatalytic synthesis of 20G	enzymatic product from 20, CDCl3, 0.05% TMS, 25 °C	contains minor unidentified impurities	1H NMR (400 MHz)
AfG3PS_NMR_157	Biocatalytic synthesis of 20G	enzymatic product from 20, CDCl3, 0.05% TMS, 25 °C	contains minor unidentified impurities	1H NMR (700 MHz)
AfG3PS_NMR_158	Biocatalytic synthesis of 20G	enzymatic product from 20, CDCl3, 0.05% TMS, 25 °C	contains minor unidentified impurities	1H,1H COSY
AfG3PS_NMR_159	Biocatalytic synthesis of 20G	enzymatic product from 20, CDCl3, 0.05% TMS, 25 °C	contains minor unidentified impurities	1H,1H NOESY
AfG3PS_NMR_160	Biocatalytic synthesis of 21G	enzymatic product from 21, CDCl3, 0.05% TMS, 25 °C	contains 20G and unidentified impurities	1H NMR (400 MHz)
AfG3PS_NMR_161	Biocatalytic synthesis of 21G	enzymatic product from 21, CDCl3, 0.05% TMS, 25 °C	contains 20G and unidentified impurities	1H NMR (700 MHz)
AfG3PS_NMR_162	Biocatalytic synthesis of 21G	enzymatic product from 21, CDCl3, 0.05% TMS, 25 °C	contains 20G and unidentified impurities	1H,1H COSY
AfG3PS_NMR_163	Biocatalytic synthesis of 21G	enzymatic product from 21, CDCl3, 0.05% TMS, 25 °C	contains 20G and unidentified impurities	1H,1H NOESY
AfG3PS_NMR_164	Telescoped synthesis of 3G	ca. 2 mg product crude product, CDCl3, 0.05% TMS, 25 °C	crude product	1H NMR
				
Characterization of derivatized products (boronates)				
AfG3PS_NMR_165	Synthesis of rac-2B, Scheme S2	derivatization product from rac-2G, CDCl3, 0.05% TMS, 25 °C	crude derivatized material	1H NMR
AfG3PS_NMR_166	Synthesis of rac-2B, Scheme S2	derivatization product from rac-2G, CDCl3, 0.05% TMS, 25 °C	crude derivatized material	13C NMR
AfG3PS_NMR_167	Synthesis of rac-2B, Scheme S2	derivatization product from rac-2G, CDCl3, 0.05% TMS, 25 °C	crude derivatized material	13C-Dept135
AfG3PS_NMR_168	Synthesis of rac-2B, Scheme S2	derivatization product from rac-2G, CDCl3, 0.05% TMS, 25 °C	crude derivatized material	1H,1H COSY
AfG3PS_NMR_169	Synthesis of rac-2B, Scheme S2	derivatization product from rac-2G, CDCl3, 0.05% TMS, 25 °C	crude derivatized material	1H,13C HMBC
AfG3PS_NMR_170	Synthesis of R-2B, Scheme S2	derivatization product from R-2G, CDCl3, 0.05% TMS, 25 °C	crude derivatized material	1H NMR
AfG3PS_NMR_171	Synthesis of 5B, Figure 2a	derivatization product from 5G, CDCl3, 0.05% TMS, 25 °C	crude derivatized material	1H NMR
AfG3PS_NMR_172	Figure S32	derivatization product from enzymatic 2G, CDCl3, 0.05% TMS, 25 °C	crude derivatized material	1H NMR
AfG3PS_NMR_173	Figure S32, Figure 2a	derivatization product from enzymatic 2G from 15:85 S/R-GP, CDCl3, 0.05% TMS, 25 °C	crude derivatized material	1H NMR
AfG3PS_NMR_174	Figure S32	derivatization product from enzymatic E-3G, CDCl3, 0.05% TMS, 25 °C	crude derivatized material	1H NMR
AfG3PS_NMR_175	Figure S32, Figure 2a	derivatization product from enzymatic Z-3G, CDCl3, 0.05% TMS, 25 °C	crude derivatized material	1H NMR
AfG3PS_NMR_176	Figure S32, Figure 2a	derivatization product from enzymatic 6G, CDCl3, 0.05% TMS, 25 °C	crude derivatized material	1H NMR
AfG3PS_NMR_177	Figure S32	derivatization product from enzymatic 10G, CDCl3, 0.05% TMS, 25 °C	crude derivatized material	1H NMR
AfG3PS_NMR_178	Figure S32	derivatization product from enzymatic 18G, CDCl3, 0.05% TMS, 25 °C	crude derivatized material	1H NMR
AfG3PS_NMR_179	Figure S32, Figure 2a	derivatization product from enzymatic 19G, CDCl3, 0.05% TMS, 25 °C	crude derivatized material	1H NMR
AfG3PS_NMR_180	Figure S32	derivatization product from enzymatic 20G, CDCl3, 0.05% TMS, 25 °C	crude derivatized material	1H NMR
AfG3PS_NMR_181	Figure S32	derivatization product from enzymatic 21G, CDCl3, 0.05% TMS, 25 °C	crude derivatized material	1H NMR
AfG3PS_NMR_182	Figure S30b	derivatization product from 3G from AfG3PS D15A, CDCl3, 0.05% TMS, 25 °C	crude derivatized material	1H NMR
AfG3PS_NMR_183	Figure S30b	derivatization product from 3G from AfG3PS R16A, CDCl3, 0.05% TMS, 25 °C	crude derivatized material	1H NMR
AfG3PS_NMR_184	Figure S30b	derivatization product from 3G from AfG3PS S37A, CDCl3, 0.05% TMS, 25 °C	crude derivatized material	1H NMR
AfG3PS_NMR_185	Figure S30b	derivatization product from 3G from AfG3PS Y124F, CDCl3, 0.05% TMS, 25 °C	crude derivatized material	1H NMR
AfG3PS_NMR_186	Figure S30b	derivatization product from 3G from AfG3PS S131A, CDCl3, 0.05% TMS, 25 °C	crude derivatized material	1H NMR
AfG3PS_NMR_187	Figure S30b	derivatization product from 3G from AfG3PS E167D, CDCl3, 0.05% TMS, 25 °C	crude derivatized material	1H NMR
AfG3PS_NMR_188	Figure S30b	derivatization product from 3G from AfG3PS S169A, CDCl3, 0.05% TMS, 25 °C	crude derivatized material	1H NMR
AfG3PS_NMR_189	Figure S30b	derivatization product from 3G from AfG3PS E220A, CDCl3, 0.05% TMS, 25 °C	crude derivatized material	1H NMR
				
Titration of AfG3PS with GP				
AfG3PS_NMR_190	Figure S23a	1 mM rac-GP, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	0 µM AfG3PS	1H NMR
AfG3PS_NMR_191	Figure S23a	1 mM rac-GP, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	10 µM AfG3PS	1H NMR
AfG3PS_NMR_192	Figure S23a	1 mM rac-GP, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	20 µM AfG3PS	1H NMR
AfG3PS_NMR_193	Figure S23a	1 mM rac-GP, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	50 µM AfG3PS	1H NMR
AfG3PS_NMR_194	Figure S23a	1 mM rac-GP, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	100 µM AfG3PS	1H NMR
AfG3PS_NMR_195	Figure S23a	1 mM rac-GP, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	200 µM AfG3PS	1H NMR
				
1H NMR kinetics				
AfG3PS_NMR_196	Figure S19	crude product from etherification and dephosphorylation of 20, CDCl3, 0.05% TMS, 25 °C	0 mM MgCl2	1H NMR
AfG3PS_NMR_197	Figure S19 & S20	crude product from etherification and dephosphorylation of 20, CDCl3, 0.05% TMS, 25 °C	0.7 mM MgCl2	1H NMR
AfG3PS_NMR_198	Figure S19 & S20	crude product from etherification and dephosphorylation of 20, CDCl3, 0.05% TMS, 25 °C	2 mM MgCl2	1H NMR
AfG3PS_NMR_199	Figure S19 & S20	crude product from etherification and dephosphorylation of 20, CDCl3, 0.05% TMS, 25 °C	4 mM MgCl2	1H NMR
				
31P NMR kinetics				
varied [AfG3PS]				
AfG3PS_NMR_200	Figure S18c+d	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 0 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	0 h	31P NMR
AfG3PS_NMR_201	Figure S18c+d	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 0 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	1 h	31P NMR
AfG3PS_NMR_202	Figure S18c+d	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 0 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	2 h	31P NMR
AfG3PS_NMR_203	Figure S18c+d	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 0 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	3 h	31P NMR
AfG3PS_NMR_204	Figure S18c+d	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 0 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	4 h	31P NMR
AfG3PS_NMR_205	Figure S18c+d	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 0 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	5 h	31P NMR
AfG3PS_NMR_206	Figure S18c+d	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 0 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	6 h	31P NMR
AfG3PS_NMR_207	Figure S18c+d	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 0 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	7 h	31P NMR
AfG3PS_NMR_208	Figure S18c+d	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 0 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	8 h	31P NMR
AfG3PS_NMR_209	Figure S18c+d	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 0 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	9 h	31P NMR
AfG3PS_NMR_210	Figure S18c+d	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 0 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	10 h	31P NMR
AfG3PS_NMR_211	Figure S18c+d	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 0 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	11 h	31P NMR
AfG3PS_NMR_212	Figure S18c+d	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 0 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	12 h	31P NMR
AfG3PS_NMR_213	Figure S18c+d	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 2 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	0 h	31P NMR
AfG3PS_NMR_214	Figure S18c+d	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 2 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	1 h	31P NMR
AfG3PS_NMR_215	Figure S18c+d	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 2 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	2 h	31P NMR
AfG3PS_NMR_216	Figure S18c+d	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 2 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	3 h	31P NMR
AfG3PS_NMR_217	Figure S18c+d	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 2 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	4 h	31P NMR
AfG3PS_NMR_218	Figure S18c+d	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 2 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	5 h	31P NMR
AfG3PS_NMR_219	Figure S18c+d	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 2 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	6 h	31P NMR
AfG3PS_NMR_220	Figure S18c+d	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 2 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	7 h	31P NMR
AfG3PS_NMR_221	Figure S18c+d	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 2 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	8 h	31P NMR
AfG3PS_NMR_222	Figure S18c+d	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 2 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	9 h	31P NMR
AfG3PS_NMR_223	Figure S18c+d	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 2 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	10 h	31P NMR
AfG3PS_NMR_224	Figure S18c+d	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 2 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	11 h	31P NMR
AfG3PS_NMR_225	Figure S18c+d	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 2 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	12 h	31P NMR
AfG3PS_NMR_226	Figure S18c+d	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 5 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	0 h	31P NMR
AfG3PS_NMR_227	Figure S18c+d	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 5 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	1 h	31P NMR
AfG3PS_NMR_228	Figure S18c+d	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 5 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	2 h	31P NMR
AfG3PS_NMR_229	Figure S18c+d	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 5 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	3 h	31P NMR
AfG3PS_NMR_230	Figure S18c+d	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 5 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	4 h	31P NMR
AfG3PS_NMR_231	Figure S18c+d	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 5 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	5 h	31P NMR
AfG3PS_NMR_232	Figure S18c+d	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 5 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	6 h	31P NMR
AfG3PS_NMR_233	Figure S18c+d	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 5 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	7 h	31P NMR
AfG3PS_NMR_234	Figure S18c+d	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 5 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	8 h	31P NMR
AfG3PS_NMR_235	Figure S18c+d	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 5 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	9 h	31P NMR
AfG3PS_NMR_236	Figure S18c+d	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 5 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	10 h	31P NMR
AfG3PS_NMR_237	Figure S18c+d	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 5 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	11 h	31P NMR
AfG3PS_NMR_238	Figure S18c+d	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 5 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	12 h	31P NMR
AfG3PS_NMR_239	Figure S18c+d	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 10 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	0 h	31P NMR
AfG3PS_NMR_240	Figure S18c+d	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 10 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	1 h	31P NMR
AfG3PS_NMR_241	Figure S18c+d	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 10 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	2 h	31P NMR
AfG3PS_NMR_242	Figure S18c+d	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 10 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	3 h	31P NMR
AfG3PS_NMR_243	Figure S18c+d	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 10 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	4 h	31P NMR
AfG3PS_NMR_244	Figure S18c+d	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 10 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	5 h	31P NMR
AfG3PS_NMR_245	Figure S18c+d	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 10 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	6 h	31P NMR
AfG3PS_NMR_246	Figure S18c+d	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 10 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	7 h	31P NMR
AfG3PS_NMR_247	Figure S18c+d	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 10 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	8 h	31P NMR
AfG3PS_NMR_248	Figure S18c+d	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 10 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	9 h	31P NMR
AfG3PS_NMR_249	Figure S18c+d	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 10 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	10 h	31P NMR
AfG3PS_NMR_250	Figure S18c+d	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 10 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	11 h	31P NMR
AfG3PS_NMR_251	Figure S18c+d	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 10 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	12 h	31P NMR
AfG3PS_NMR_252	Figure S18c+d	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	0 h	31P NMR
AfG3PS_NMR_253	Figure S18c+d	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	1 h	31P NMR
AfG3PS_NMR_254	Figure S18c+d	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	2 h	31P NMR
AfG3PS_NMR_255	Figure S18c+d	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	3 h	31P NMR
AfG3PS_NMR_256	Figure S18c+d	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	4 h	31P NMR
AfG3PS_NMR_257	Figure S18c+d	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	5 h	31P NMR
AfG3PS_NMR_258	Figure S18c+d	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	6 h	31P NMR
AfG3PS_NMR_259	Figure S18c+d	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	7 h	31P NMR
AfG3PS_NMR_260	Figure S18c+d	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	8 h	31P NMR
AfG3PS_NMR_261	Figure S18c+d	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	9 h	31P NMR
AfG3PS_NMR_262	Figure S18c+d	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	10 h	31P NMR
AfG3PS_NMR_263	Figure S18c+d	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	11 h	31P NMR
AfG3PS_NMR_264	Figure S18c+d	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	12 h	31P NMR
AfG3PS_NMR_265	Figure S18c+d	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 50 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	0 h	31P NMR
AfG3PS_NMR_266	Figure S18c+d	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 50 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	1 h	31P NMR
AfG3PS_NMR_267	Figure S18c+d	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 50 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	2 h	31P NMR
AfG3PS_NMR_268	Figure S18c+d	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 50 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	3 h	31P NMR
AfG3PS_NMR_269	Figure S18c+d	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 50 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	4 h	31P NMR
AfG3PS_NMR_270	Figure S18c+d	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 50 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	5 h	31P NMR
AfG3PS_NMR_271	Figure S18c+d	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 50 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	6 h	31P NMR
AfG3PS_NMR_272	Figure S18c+d	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 50 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	7 h	31P NMR
AfG3PS_NMR_273	Figure S18c+d	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 50 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	8 h	31P NMR
AfG3PS_NMR_274	Figure S18c+d	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 50 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	9 h	31P NMR
AfG3PS_NMR_275	Figure S18c+d	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 50 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	10 h	31P NMR
AfG3PS_NMR_276	Figure S18c+d	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 50 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	11 h	31P NMR
AfG3PS_NMR_277	Figure S18c+d	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 50 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	12 h	31P NMR
varied [MgCl2] with 3				
AfG3PS_NMR_278	Figure S18e+f	500 µM compound 3, 2 mM rac-GP, 0.4 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	0 h	31P NMR
AfG3PS_NMR_279	Figure S18e+f	500 µM compound 3, 2 mM rac-GP, 0.4 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	1 h	31P NMR
AfG3PS_NMR_280	Figure S18e+f	500 µM compound 3, 2 mM rac-GP, 0.4 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	2 h	31P NMR
AfG3PS_NMR_281	Figure S18e+f	500 µM compound 3, 2 mM rac-GP, 0.4 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	3 h	31P NMR
AfG3PS_NMR_282	Figure S18e+f	500 µM compound 3, 2 mM rac-GP, 0.4 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	4 h	31P NMR
AfG3PS_NMR_283	Figure S18e+f	500 µM compound 3, 2 mM rac-GP, 0.4 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	5 h	31P NMR
AfG3PS_NMR_284	Figure S18e+f	500 µM compound 3, 2 mM rac-GP, 0.4 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	6 h	31P NMR
AfG3PS_NMR_285	Figure S18e+f	500 µM compound 3, 2 mM rac-GP, 0.4 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	7 h	31P NMR
AfG3PS_NMR_286	Figure S18e+f	500 µM compound 3, 2 mM rac-GP, 0.4 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	8 h	31P NMR
AfG3PS_NMR_287	Figure S18e+f	500 µM compound 3, 2 mM rac-GP, 0.4 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	9 h	31P NMR
AfG3PS_NMR_288	Figure S18e+f	500 µM compound 3, 2 mM rac-GP, 0.4 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	10 h	31P NMR
AfG3PS_NMR_289	Figure S18e+f	500 µM compound 3, 2 mM rac-GP, 0.4 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	11 h	31P NMR
AfG3PS_NMR_290	Figure S18e+f	500 µM compound 3, 2 mM rac-GP, 0.7 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	0 h	31P NMR
AfG3PS_NMR_291	Figure S18e+f	500 µM compound 3, 2 mM rac-GP, 0.7 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	1 h	31P NMR
AfG3PS_NMR_292	Figure S18e+f	500 µM compound 3, 2 mM rac-GP, 0.7 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	2 h	31P NMR
AfG3PS_NMR_293	Figure S18e+f	500 µM compound 3, 2 mM rac-GP, 0.7 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	3 h	31P NMR
AfG3PS_NMR_294	Figure S18e+f	500 µM compound 3, 2 mM rac-GP, 0.7 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	4 h	31P NMR
AfG3PS_NMR_295	Figure S18e+f	500 µM compound 3, 2 mM rac-GP, 0.7 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	5 h	31P NMR
AfG3PS_NMR_296	Figure S18e+f	500 µM compound 3, 2 mM rac-GP, 0.7 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	6 h	31P NMR
AfG3PS_NMR_297	Figure S18e+f	500 µM compound 3, 2 mM rac-GP, 0.7 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	7 h	31P NMR
AfG3PS_NMR_298	Figure S18e+f	500 µM compound 3, 2 mM rac-GP, 0.7 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	8 h	31P NMR
AfG3PS_NMR_299	Figure S18e+f	500 µM compound 3, 2 mM rac-GP, 0.7 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	9 h	31P NMR
AfG3PS_NMR_300	Figure S18e+f	500 µM compound 3, 2 mM rac-GP, 0.7 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	10 h	31P NMR
AfG3PS_NMR_301	Figure S18e+f	500 µM compound 3, 2 mM rac-GP, 0.7 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	11 h	31P NMR
AfG3PS_NMR_302	Figure S18e+f	500 µM compound 3, 2 mM rac-GP, 1.2 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	0 h	31P NMR
AfG3PS_NMR_303	Figure S18e+f	500 µM compound 3, 2 mM rac-GP, 1.2 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	1 h	31P NMR
AfG3PS_NMR_304	Figure S18e+f	500 µM compound 3, 2 mM rac-GP, 1.2 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	2 h	31P NMR
AfG3PS_NMR_305	Figure S18e+f	500 µM compound 3, 2 mM rac-GP, 1.2 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	3 h	31P NMR
AfG3PS_NMR_306	Figure S18e+f	500 µM compound 3, 2 mM rac-GP, 1.2 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	4 h	31P NMR
AfG3PS_NMR_307	Figure S18e+f	500 µM compound 3, 2 mM rac-GP, 1.2 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	5 h	31P NMR
AfG3PS_NMR_308	Figure S18e+f	500 µM compound 3, 2 mM rac-GP, 1.2 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	6 h	31P NMR
AfG3PS_NMR_309	Figure S18e+f	500 µM compound 3, 2 mM rac-GP, 1.2 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	7 h	31P NMR
AfG3PS_NMR_310	Figure S18e+f	500 µM compound 3, 2 mM rac-GP, 1.2 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	8 h	31P NMR
AfG3PS_NMR_311	Figure S18e+f	500 µM compound 3, 2 mM rac-GP, 1.2 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	9 h	31P NMR
AfG3PS_NMR_312	Figure S18e+f	500 µM compound 3, 2 mM rac-GP, 1.2 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	10 h	31P NMR
AfG3PS_NMR_313	Figure S18e+f	500 µM compound 3, 2 mM rac-GP, 1.2 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	11 h	31P NMR
AfG3PS_NMR_314	Figure S18e+f	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	0 h	31P NMR
AfG3PS_NMR_315	Figure S18e+f	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	1 h	31P NMR
AfG3PS_NMR_316	Figure S18e+f	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	2 h	31P NMR
AfG3PS_NMR_317	Figure S18e+f	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	3 h	31P NMR
AfG3PS_NMR_318	Figure S18e+f	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	4 h	31P NMR
AfG3PS_NMR_319	Figure S18e+f	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	5 h	31P NMR
AfG3PS_NMR_320	Figure S18e+f	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	6 h	31P NMR
AfG3PS_NMR_321	Figure S18e+f	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	7 h	31P NMR
AfG3PS_NMR_322	Figure S18e+f	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	8 h	31P NMR
AfG3PS_NMR_323	Figure S18e+f	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	9 h	31P NMR
AfG3PS_NMR_324	Figure S18e+f	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	10 h	31P NMR
AfG3PS_NMR_325	Figure S18e+f	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	11 h	31P NMR
AfG3PS_NMR_326	Figure S18e+f	500 µM compound 3, 2 mM rac-GP, 4 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	0 h	31P NMR
AfG3PS_NMR_327	Figure S18e+f	500 µM compound 3, 2 mM rac-GP, 4 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	1 h	31P NMR
AfG3PS_NMR_328	Figure S18e+f	500 µM compound 3, 2 mM rac-GP, 4 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	2 h	31P NMR
AfG3PS_NMR_329	Figure S18e+f	500 µM compound 3, 2 mM rac-GP, 4 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	3 h	31P NMR
AfG3PS_NMR_330	Figure S18e+f	500 µM compound 3, 2 mM rac-GP, 4 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	4 h	31P NMR
AfG3PS_NMR_331	Figure S18e+f	500 µM compound 3, 2 mM rac-GP, 4 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	5 h	31P NMR
AfG3PS_NMR_332	Figure S18e+f	500 µM compound 3, 2 mM rac-GP, 4 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	6 h	31P NMR
AfG3PS_NMR_333	Figure S18e+f	500 µM compound 3, 2 mM rac-GP, 4 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	7 h	31P NMR
AfG3PS_NMR_334	Figure S18e+f	500 µM compound 3, 2 mM rac-GP, 4 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	8 h	31P NMR
AfG3PS_NMR_335	Figure S18e+f	500 µM compound 3, 2 mM rac-GP, 4 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	9 h	31P NMR
AfG3PS_NMR_336	Figure S18e+f	500 µM compound 3, 2 mM rac-GP, 4 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	10 h	31P NMR
AfG3PS_NMR_337	Figure S18e+f	500 µM compound 3, 2 mM rac-GP, 4 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	11 h	31P NMR
AfG3PS_NMR_338	Figure S18e+f	500 µM compound 3, 2 mM rac-GP, 7 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	0 h	31P NMR
AfG3PS_NMR_339	Figure S18e+f	500 µM compound 3, 2 mM rac-GP, 7 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	1 h	31P NMR
AfG3PS_NMR_340	Figure S18e+f	500 µM compound 3, 2 mM rac-GP, 7 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	2 h	31P NMR
AfG3PS_NMR_341	Figure S18e+f	500 µM compound 3, 2 mM rac-GP, 7 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	3 h	31P NMR
AfG3PS_NMR_342	Figure S18e+f	500 µM compound 3, 2 mM rac-GP, 7 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	4 h	31P NMR
AfG3PS_NMR_343	Figure S18e+f	500 µM compound 3, 2 mM rac-GP, 7 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	5 h	31P NMR
AfG3PS_NMR_344	Figure S18e+f	500 µM compound 3, 2 mM rac-GP, 7 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	6 h	31P NMR
AfG3PS_NMR_345	Figure S18e+f	500 µM compound 3, 2 mM rac-GP, 7 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	7 h	31P NMR
AfG3PS_NMR_346	Figure S18e+f	500 µM compound 3, 2 mM rac-GP, 7 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	8 h	31P NMR
AfG3PS_NMR_347	Figure S18e+f	500 µM compound 3, 2 mM rac-GP, 7 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	9 h	31P NMR
AfG3PS_NMR_348	Figure S18e+f	500 µM compound 3, 2 mM rac-GP, 7 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	10 h	31P NMR
AfG3PS_NMR_349	Figure S18e+f	500 µM compound 3, 2 mM rac-GP, 7 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	11 h	31P NMR
AfG3PS_NMR_350	Figure S18e+f	500 µM compound 3, 10 mM rac-GP, 0.4 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	0 h	31P NMR
AfG3PS_NMR_351	Figure S18e+f	500 µM compound 3, 10 mM rac-GP, 0.4 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	1 h	31P NMR
AfG3PS_NMR_352	Figure S18e+f	500 µM compound 3, 10 mM rac-GP, 0.4 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	2 h	31P NMR
AfG3PS_NMR_353	Figure S18e+f	500 µM compound 3, 10 mM rac-GP, 0.4 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	3 h	31P NMR
AfG3PS_NMR_354	Figure S18e+f	500 µM compound 3, 10 mM rac-GP, 0.4 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	4 h	31P NMR
AfG3PS_NMR_355	Figure S18e+f	500 µM compound 3, 10 mM rac-GP, 0.4 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	5 h	31P NMR
AfG3PS_NMR_356	Figure S18e+f	500 µM compound 3, 10 mM rac-GP, 0.4 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	6 h	31P NMR
AfG3PS_NMR_357	Figure S18e+f	500 µM compound 3, 10 mM rac-GP, 0.4 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	7 h	31P NMR
AfG3PS_NMR_358	Figure S18e+f	500 µM compound 3, 10 mM rac-GP, 0.4 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	8 h	31P NMR
AfG3PS_NMR_359	Figure S18e+f	500 µM compound 3, 10 mM rac-GP, 0.4 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	9 h	31P NMR
AfG3PS_NMR_360	Figure S18e+f	500 µM compound 3, 10 mM rac-GP, 0.4 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	10 h	31P NMR
AfG3PS_NMR_361	Figure S18e+f	500 µM compound 3, 10 mM rac-GP, 0.4 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	11 h	31P NMR
AfG3PS_NMR_362	Figure S18e+f	500 µM compound 3, 10 mM rac-GP, 0.4 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	12 h	31P NMR
AfG3PS_NMR_363	Figure S18e+f	500 µM compound 3, 10 mM rac-GP, 0.7 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	0 h	31P NMR
AfG3PS_NMR_364	Figure S18e+f	500 µM compound 3, 10 mM rac-GP, 0.7 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	1 h	31P NMR
AfG3PS_NMR_365	Figure S18e+f	500 µM compound 3, 10 mM rac-GP, 0.7 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	2 h	31P NMR
AfG3PS_NMR_366	Figure S18e+f	500 µM compound 3, 10 mM rac-GP, 0.7 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	3 h	31P NMR
AfG3PS_NMR_367	Figure S18e+f	500 µM compound 3, 10 mM rac-GP, 0.7 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	4 h	31P NMR
AfG3PS_NMR_368	Figure S18e+f	500 µM compound 3, 10 mM rac-GP, 0.7 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	5 h	31P NMR
AfG3PS_NMR_369	Figure S18e+f	500 µM compound 3, 10 mM rac-GP, 0.7 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	6 h	31P NMR
AfG3PS_NMR_370	Figure S18e+f	500 µM compound 3, 10 mM rac-GP, 0.7 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	7 h	31P NMR
AfG3PS_NMR_371	Figure S18e+f	500 µM compound 3, 10 mM rac-GP, 0.7 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	8 h	31P NMR
AfG3PS_NMR_372	Figure S18e+f	500 µM compound 3, 10 mM rac-GP, 0.7 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	9 h	31P NMR
AfG3PS_NMR_373	Figure S18e+f	500 µM compound 3, 10 mM rac-GP, 0.7 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	10 h	31P NMR
AfG3PS_NMR_374	Figure S18e+f	500 µM compound 3, 10 mM rac-GP, 0.7 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	11 h	31P NMR
AfG3PS_NMR_375	Figure S18e+f	500 µM compound 3, 10 mM rac-GP, 0.7 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	12 h	31P NMR
AfG3PS_NMR_376	Figure S18e+f	500 µM compound 3, 10 mM rac-GP, 1.2 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	0 h	31P NMR
AfG3PS_NMR_377	Figure S18e+f	500 µM compound 3, 10 mM rac-GP, 1.2 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	1 h	31P NMR
AfG3PS_NMR_378	Figure S18e+f	500 µM compound 3, 10 mM rac-GP, 1.2 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	2 h	31P NMR
AfG3PS_NMR_379	Figure S18e+f	500 µM compound 3, 10 mM rac-GP, 1.2 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	3 h	31P NMR
AfG3PS_NMR_380	Figure S18e+f	500 µM compound 3, 10 mM rac-GP, 1.2 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	4 h	31P NMR
AfG3PS_NMR_381	Figure S18e+f	500 µM compound 3, 10 mM rac-GP, 1.2 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	5 h	31P NMR
AfG3PS_NMR_382	Figure S18e+f	500 µM compound 3, 10 mM rac-GP, 1.2 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	6 h	31P NMR
AfG3PS_NMR_383	Figure S18e+f	500 µM compound 3, 10 mM rac-GP, 1.2 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	7 h	31P NMR
AfG3PS_NMR_384	Figure S18e+f	500 µM compound 3, 10 mM rac-GP, 1.2 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	8 h	31P NMR
AfG3PS_NMR_385	Figure S18e+f	500 µM compound 3, 10 mM rac-GP, 1.2 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	9 h	31P NMR
AfG3PS_NMR_386	Figure S18e+f	500 µM compound 3, 10 mM rac-GP, 1.2 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	10 h	31P NMR
AfG3PS_NMR_387	Figure S18e+f	500 µM compound 3, 10 mM rac-GP, 1.2 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	11 h	31P NMR
AfG3PS_NMR_388	Figure S18e+f	500 µM compound 3, 10 mM rac-GP, 1.2 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	12 h	31P NMR
AfG3PS_NMR_389	Figure S18e+f	500 µM compound 3, 10 mM rac-GP, 2 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	0 h	31P NMR
AfG3PS_NMR_390	Figure S18e+f	500 µM compound 3, 10 mM rac-GP, 2 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	1 h	31P NMR
AfG3PS_NMR_391	Figure S18e+f	500 µM compound 3, 10 mM rac-GP, 2 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	2 h	31P NMR
AfG3PS_NMR_392	Figure S18e+f	500 µM compound 3, 10 mM rac-GP, 2 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	3 h	31P NMR
AfG3PS_NMR_393	Figure S18e+f	500 µM compound 3, 10 mM rac-GP, 2 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	4 h	31P NMR
AfG3PS_NMR_394	Figure S18e+f	500 µM compound 3, 10 mM rac-GP, 2 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	5 h	31P NMR
AfG3PS_NMR_395	Figure S18e+f	500 µM compound 3, 10 mM rac-GP, 2 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	6 h	31P NMR
AfG3PS_NMR_396	Figure S18e+f	500 µM compound 3, 10 mM rac-GP, 2 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	7 h	31P NMR
AfG3PS_NMR_397	Figure S18e+f	500 µM compound 3, 10 mM rac-GP, 2 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	8 h	31P NMR
AfG3PS_NMR_398	Figure S18e+f	500 µM compound 3, 10 mM rac-GP, 2 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	9 h	31P NMR
AfG3PS_NMR_399	Figure S18e+f	500 µM compound 3, 10 mM rac-GP, 2 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	10 h	31P NMR
AfG3PS_NMR_400	Figure S18e+f	500 µM compound 3, 10 mM rac-GP, 2 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	11 h	31P NMR
AfG3PS_NMR_401	Figure S18e+f	500 µM compound 3, 10 mM rac-GP, 2 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	12 h	31P NMR
AfG3PS_NMR_402	Figure S18e+f	500 µM compound 3, 10 mM rac-GP, 4 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	0 h	31P NMR
AfG3PS_NMR_403	Figure S18e+f	500 µM compound 3, 10 mM rac-GP, 4 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	1 h	31P NMR
AfG3PS_NMR_404	Figure S18e+f	500 µM compound 3, 10 mM rac-GP, 4 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	2 h	31P NMR
AfG3PS_NMR_405	Figure S18e+f	500 µM compound 3, 10 mM rac-GP, 4 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	3 h	31P NMR
AfG3PS_NMR_406	Figure S18e+f	500 µM compound 3, 10 mM rac-GP, 4 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	4 h	31P NMR
AfG3PS_NMR_407	Figure S18e+f	500 µM compound 3, 10 mM rac-GP, 4 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	5 h	31P NMR
AfG3PS_NMR_408	Figure S18e+f	500 µM compound 3, 10 mM rac-GP, 4 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	6 h	31P NMR
AfG3PS_NMR_409	Figure S18e+f	500 µM compound 3, 10 mM rac-GP, 4 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	7 h	31P NMR
AfG3PS_NMR_410	Figure S18e+f	500 µM compound 3, 10 mM rac-GP, 4 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	8 h	31P NMR
AfG3PS_NMR_411	Figure S18e+f	500 µM compound 3, 10 mM rac-GP, 4 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	9 h	31P NMR
AfG3PS_NMR_412	Figure S18e+f	500 µM compound 3, 10 mM rac-GP, 4 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	10 h	31P NMR
AfG3PS_NMR_413	Figure S18e+f	500 µM compound 3, 10 mM rac-GP, 4 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	11 h	31P NMR
AfG3PS_NMR_414	Figure S18e+f	500 µM compound 3, 10 mM rac-GP, 4 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	12 h	31P NMR
AfG3PS_NMR_415	Figure S18e+f	500 µM compound 3, 10 mM rac-GP, 7 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	0 h	31P NMR
AfG3PS_NMR_416	Figure S18e+f	500 µM compound 3, 10 mM rac-GP, 7 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	1 h	31P NMR
AfG3PS_NMR_417	Figure S18e+f	500 µM compound 3, 10 mM rac-GP, 7 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	2 h	31P NMR
AfG3PS_NMR_418	Figure S18e+f	500 µM compound 3, 10 mM rac-GP, 7 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	3 h	31P NMR
AfG3PS_NMR_419	Figure S18e+f	500 µM compound 3, 10 mM rac-GP, 7 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	4 h	31P NMR
AfG3PS_NMR_420	Figure S18e+f	500 µM compound 3, 10 mM rac-GP, 7 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	5 h	31P NMR
AfG3PS_NMR_421	Figure S18e+f	500 µM compound 3, 10 mM rac-GP, 7 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	6 h	31P NMR
AfG3PS_NMR_422	Figure S18e+f	500 µM compound 3, 10 mM rac-GP, 7 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	7 h	31P NMR
AfG3PS_NMR_423	Figure S18e+f	500 µM compound 3, 10 mM rac-GP, 7 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	8 h	31P NMR
AfG3PS_NMR_424	Figure S18e+f	500 µM compound 3, 10 mM rac-GP, 7 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	9 h	31P NMR
AfG3PS_NMR_425	Figure S18e+f	500 µM compound 3, 10 mM rac-GP, 7 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	10 h	31P NMR
AfG3PS_NMR_426	Figure S18e+f	500 µM compound 3, 10 mM rac-GP, 7 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	11 h	31P NMR
AfG3PS_NMR_427	Figure S18e+f	500 µM compound 3, 10 mM rac-GP, 7 mM MgCl2, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	12 h	31P NMR
varied [MgCl2] with 20				
AfG3PS_NMR_428	Figure S18g+h	500 µM compound 20, 2 mM rac-GP, 0.4 mM MgCl2, 200 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	0 h	31P NMR
AfG3PS_NMR_429	Figure S18g+h	500 µM compound 20, 2 mM rac-GP, 0.4 mM MgCl2, 200 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	1.5 h	31P NMR
AfG3PS_NMR_430	Figure S18g+h	500 µM compound 20, 2 mM rac-GP, 0.4 mM MgCl2, 200 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	3 h	31P NMR
AfG3PS_NMR_431	Figure S18g+h	500 µM compound 20, 2 mM rac-GP, 0.4 mM MgCl2, 200 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	4.5 h	31P NMR
AfG3PS_NMR_432	Figure S18g+h	500 µM compound 20, 2 mM rac-GP, 0.4 mM MgCl2, 200 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	6 h	31P NMR
AfG3PS_NMR_433	Figure S18g+h	500 µM compound 20, 2 mM rac-GP, 0.4 mM MgCl2, 200 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	7.5 h	31P NMR
AfG3PS_NMR_434	Figure S18g+h	500 µM compound 20, 2 mM rac-GP, 0.4 mM MgCl2, 200 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	9 h	31P NMR
AfG3PS_NMR_435	Figure S18g+h	500 µM compound 20, 2 mM rac-GP, 0.4 mM MgCl2, 200 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	10.5 h	31P NMR
AfG3PS_NMR_436	Figure S18g+h	500 µM compound 20, 2 mM rac-GP, 0.4 mM MgCl2, 200 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	12 h	31P NMR
AfG3PS_NMR_437	Figure S18g+h	500 µM compound 20, 2 mM rac-GP, 0.4 mM MgCl2, 200 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	13.5 h	31P NMR
AfG3PS_NMR_438	Figure S18g+h	500 µM compound 20, 2 mM rac-GP, 0.7 mM MgCl2, 200 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	0 h	31P NMR
AfG3PS_NMR_439	Figure S18g+h	500 µM compound 20, 2 mM rac-GP, 0.7 mM MgCl2, 200 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	1.5 h	31P NMR
AfG3PS_NMR_440	Figure S18g+h	500 µM compound 20, 2 mM rac-GP, 0.7 mM MgCl2, 200 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	3 h	31P NMR
AfG3PS_NMR_441	Figure S18g+h	500 µM compound 20, 2 mM rac-GP, 0.7 mM MgCl2, 200 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	4.5 h	31P NMR
AfG3PS_NMR_442	Figure S18g+h	500 µM compound 20, 2 mM rac-GP, 0.7 mM MgCl2, 200 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	6 h	31P NMR
AfG3PS_NMR_443	Figure S18g+h	500 µM compound 20, 2 mM rac-GP, 0.7 mM MgCl2, 200 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	7.5 h	31P NMR
AfG3PS_NMR_444	Figure S18g+h	500 µM compound 20, 2 mM rac-GP, 0.7 mM MgCl2, 200 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	9 h	31P NMR
AfG3PS_NMR_445	Figure S18g+h	500 µM compound 20, 2 mM rac-GP, 0.7 mM MgCl2, 200 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	10.5 h	31P NMR
AfG3PS_NMR_446	Figure S18g+h	500 µM compound 20, 2 mM rac-GP, 0.7 mM MgCl2, 200 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	12 h	31P NMR
AfG3PS_NMR_447	Figure S18g+h	500 µM compound 20, 2 mM rac-GP, 0.7 mM MgCl2, 200 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	13.5 h	31P NMR
AfG3PS_NMR_448	Figure S18g+h	500 µM compound 20, 2 mM rac-GP, 1.2 mM MgCl2, 200 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	0 h	31P NMR
AfG3PS_NMR_449	Figure S18g+h	500 µM compound 20, 2 mM rac-GP, 1.2 mM MgCl2, 200 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	1.5 h	31P NMR
AfG3PS_NMR_450	Figure S18g+h	500 µM compound 20, 2 mM rac-GP, 1.2 mM MgCl2, 200 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	3 h	31P NMR
AfG3PS_NMR_451	Figure S18g+h	500 µM compound 20, 2 mM rac-GP, 1.2 mM MgCl2, 200 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	4.5 h	31P NMR
AfG3PS_NMR_452	Figure S18g+h	500 µM compound 20, 2 mM rac-GP, 1.2 mM MgCl2, 200 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	6 h	31P NMR
AfG3PS_NMR_453	Figure S18g+h	500 µM compound 20, 2 mM rac-GP, 1.2 mM MgCl2, 200 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	7.5 h	31P NMR
AfG3PS_NMR_454	Figure S18g+h	500 µM compound 20, 2 mM rac-GP, 1.2 mM MgCl2, 200 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	9 h	31P NMR
AfG3PS_NMR_455	Figure S18g+h	500 µM compound 20, 2 mM rac-GP, 1.2 mM MgCl2, 200 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	10.5 h	31P NMR
AfG3PS_NMR_456	Figure S18g+h	500 µM compound 20, 2 mM rac-GP, 1.2 mM MgCl2, 200 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	12 h	31P NMR
AfG3PS_NMR_457	Figure S18g+h	500 µM compound 20, 2 mM rac-GP, 1.2 mM MgCl2, 200 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	13.5 h	31P NMR
AfG3PS_NMR_458	Figure S18g+h	500 µM compound 20, 2 mM rac-GP, 2 mM MgCl2, 200 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	0 h	31P NMR
AfG3PS_NMR_459	Figure S18g+h	500 µM compound 20, 2 mM rac-GP, 2 mM MgCl2, 200 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	1.5 h	31P NMR
AfG3PS_NMR_460	Figure S18g+h	500 µM compound 20, 2 mM rac-GP, 2 mM MgCl2, 200 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	3 h	31P NMR
AfG3PS_NMR_461	Figure S18g+h	500 µM compound 20, 2 mM rac-GP, 2 mM MgCl2, 200 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	4.5 h	31P NMR
AfG3PS_NMR_462	Figure S18g+h	500 µM compound 20, 2 mM rac-GP, 2 mM MgCl2, 200 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	6 h	31P NMR
AfG3PS_NMR_463	Figure S18g+h	500 µM compound 20, 2 mM rac-GP, 2 mM MgCl2, 200 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	7.5 h	31P NMR
AfG3PS_NMR_464	Figure S18g+h	500 µM compound 20, 2 mM rac-GP, 2 mM MgCl2, 200 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	9 h	31P NMR
AfG3PS_NMR_465	Figure S18g+h	500 µM compound 20, 2 mM rac-GP, 2 mM MgCl2, 200 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	10.5 h	31P NMR
AfG3PS_NMR_466	Figure S18g+h	500 µM compound 20, 2 mM rac-GP, 2 mM MgCl2, 200 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	12 h	31P NMR
AfG3PS_NMR_467	Figure S18g+h	500 µM compound 20, 2 mM rac-GP, 2 mM MgCl2, 200 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	13.5 h	31P NMR
AfG3PS_NMR_468	Figure S18g+h	500 µM compound 20, 2 mM rac-GP, 4 mM MgCl2, 200 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	0 h	31P NMR
AfG3PS_NMR_469	Figure S18g+h	500 µM compound 20, 2 mM rac-GP, 4 mM MgCl2, 200 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	1.5 h	31P NMR
AfG3PS_NMR_470	Figure S18g+h	500 µM compound 20, 2 mM rac-GP, 4 mM MgCl2, 200 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	3 h	31P NMR
AfG3PS_NMR_471	Figure S18g+h	500 µM compound 20, 2 mM rac-GP, 4 mM MgCl2, 200 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	4.5 h	31P NMR
AfG3PS_NMR_472	Figure S18g+h	500 µM compound 20, 2 mM rac-GP, 4 mM MgCl2, 200 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	6 h	31P NMR
AfG3PS_NMR_473	Figure S18g+h	500 µM compound 20, 2 mM rac-GP, 4 mM MgCl2, 200 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	7.5 h	31P NMR
AfG3PS_NMR_474	Figure S18g+h	500 µM compound 20, 2 mM rac-GP, 4 mM MgCl2, 200 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	9 h	31P NMR
AfG3PS_NMR_475	Figure S18g+h	500 µM compound 20, 2 mM rac-GP, 4 mM MgCl2, 200 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	10.5 h	31P NMR
AfG3PS_NMR_476	Figure S18g+h	500 µM compound 20, 2 mM rac-GP, 4 mM MgCl2, 200 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	12 h	31P NMR
AfG3PS_NMR_477	Figure S18g+h	500 µM compound 20, 2 mM rac-GP, 4 mM MgCl2, 200 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	13.5 h	31P NMR
AfG3PS_NMR_478	Figure S18g+h	500 µM compound 20, 2 mM rac-GP, 7 mM MgCl2, 200 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	0 h	31P NMR
AfG3PS_NMR_479	Figure S18g+h	500 µM compound 20, 2 mM rac-GP, 7 mM MgCl2, 200 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	1.5 h	31P NMR
AfG3PS_NMR_480	Figure S18g+h	500 µM compound 20, 2 mM rac-GP, 7 mM MgCl2, 200 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	3 h	31P NMR
AfG3PS_NMR_481	Figure S18g+h	500 µM compound 20, 2 mM rac-GP, 7 mM MgCl2, 200 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	4.5 h	31P NMR
AfG3PS_NMR_482	Figure S18g+h	500 µM compound 20, 2 mM rac-GP, 7 mM MgCl2, 200 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	6 h	31P NMR
AfG3PS_NMR_483	Figure S18g+h	500 µM compound 20, 2 mM rac-GP, 7 mM MgCl2, 200 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	7.5 h	31P NMR
AfG3PS_NMR_484	Figure S18g+h	500 µM compound 20, 2 mM rac-GP, 7 mM MgCl2, 200 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	9 h	31P NMR
AfG3PS_NMR_485	Figure S18g+h	500 µM compound 20, 2 mM rac-GP, 7 mM MgCl2, 200 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	10.5 h	31P NMR
AfG3PS_NMR_486	Figure S18g+h	500 µM compound 20, 2 mM rac-GP, 7 mM MgCl2, 200 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	12 h	31P NMR
AfG3PS_NMR_487	Figure S18g+h	500 µM compound 20, 2 mM rac-GP, 7 mM MgCl2, 200 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	13.5 h	31P NMR
Product inhibition				
AfG3PS_NMR_488	Figure S22	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	0 h	31P NMR
AfG3PS_NMR_489	Figure S22	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	1 h	31P NMR
AfG3PS_NMR_490	Figure S22	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	2 h	31P NMR
AfG3PS_NMR_491	Figure S22	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	3 h	31P NMR
AfG3PS_NMR_492	Figure S22	500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	4 h	31P NMR
AfG3PS_NMR_493	Figure S22	20 µM then 500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	0 h	31P NMR
AfG3PS_NMR_494	Figure S22	20 µM then 500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	1 h	31P NMR
AfG3PS_NMR_495	Figure S22	20 µM then 500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	2 h	31P NMR
AfG3PS_NMR_496	Figure S22	20 µM then 500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	3 h	31P NMR
AfG3PS_NMR_497	Figure S22	20 µM then 500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	4 h	31P NMR
AfG3PS_NMR_498	Figure S22	50 µM then 500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	0 h	31P NMR
AfG3PS_NMR_499	Figure S22	50 µM then 500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	1 h	31P NMR
AfG3PS_NMR_500	Figure S22	50 µM then 500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	2 h	31P NMR
AfG3PS_NMR_501	Figure S22	50 µM then 500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	3 h	31P NMR
AfG3PS_NMR_502	Figure S22	50 µM then 500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	4 h	31P NMR
AfG3PS_NMR_503	Figure S22	100 µM then 500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	0 h	31P NMR
AfG3PS_NMR_504	Figure S22	100 µM then 500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	1 h	31P NMR
AfG3PS_NMR_505	Figure S22	100 µM then 500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	2 h	31P NMR
AfG3PS_NMR_506	Figure S22	100 µM then 500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	3 h	31P NMR
AfG3PS_NMR_507	Figure S22	100 µM then 500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	4 h	31P NMR
AfG3PS_NMR_508	Figure S22	500 µM then 500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	0 h	31P NMR
AfG3PS_NMR_509	Figure S22	500 µM then 500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	1 h	31P NMR
AfG3PS_NMR_510	Figure S22	500 µM then 500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	2 h	31P NMR
AfG3PS_NMR_511	Figure S22	500 µM then 500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	3 h	31P NMR
AfG3PS_NMR_512	Figure S22	500 µM then 500 µM compound 3, 2 mM rac-GP, 2 mM MgCl2, 1 mM phosphorylethanolamine, 20 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	4 h	31P NMR
E/Z differentiation				
AfG3PS_NMR_513	Figure S25	2 mM compound Z-3, 5 mM rac-GP, 10 mM MgCl2, 100 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	0 h	31P NMR
AfG3PS_NMR_514	Figure S25	2 mM compound Z-3, 5 mM rac-GP, 10 mM MgCl2, 100 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	1 h	31P NMR
AfG3PS_NMR_515	Figure S25	2 mM compound Z-3, 5 mM rac-GP, 10 mM MgCl2, 100 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	2 h	31P NMR
AfG3PS_NMR_516	Figure S25	2 mM compound Z-3, 5 mM rac-GP, 10 mM MgCl2, 100 µg/mL AfG3PS, 50 mM taurine buffer, 10% D2O, 0.005% TSP, 25 °C	3 h	31P NMR