Figure 4 in Integrative microscopy to explore physical and nanomechanical properties of eggshells of diapausing embryos in Rotifera: a proof-of-concept study

Figure 4. Characterisation of the overwintering eggs of Aedes aegypti. a. Scanning Electron Microscopy of an egg. The eggshell is damaged and much of the ornamentation has been artificially removed to expose the shell surface. b. Close-up of the ornamentation of an eggshell. c. Close-up of the surface of the eggshell region without ornamentation. d. Topographic image of the external surface of an eggshell generated with Atomic Force Microscopy. e. Force–distance (FD) curve generated from a single location on an eggshell. Parameters for the position of the location on the eggshell are indicated by the cursor positions (screen shot). Maximum force (MF, green circle) and 20% maximum force (20% MF, orange circle) are indicated on the FD curve. Scale bars: A = 100 µm; B = 53 µm; C = 15 µm.