Funding provided by: Holsworth Wildlife Research Endowment - Equity Trustees Charitable Foundation & the Ecological Society of Australia*
Crossref Funder Registry ID:
Award Number:
Funding provided by: Wettenhall Environment Trust
Crossref Funder Registry ID: https://ror.org/04tvfnj07
Award Number:
Funding provided by: University of Western Australia
Crossref Funder Registry ID: https://ror.org/047272k79
Award Number:
Data was collected via environmental DNA sampling.
", "type": { "id": "methods", "title": { "de": "Methoden", "en": "Methods" } } } ], "creators": [ { "affiliations": [ { "name": "University of Western Australia" } ], "person_or_org": { "family_name": "Nordstrom", "given_name": "Bethany", "identifiers": [ { "identifier": "0000-0003-0978-9194", "scheme": "orcid" } ], "name": "Nordstrom, Bethany", "type": "personal" } }, { "affiliations": [ { "name": "Commonwealth Scientific and Industrial Research Organisation" } ], "person_or_org": { "family_name": "Budd", "given_name": "Alyssa", "name": "Budd, Alyssa", "type": "personal" } }, { "affiliations": [ { "name": "Department of Parks and Wildlife" } ], "person_or_org": { "family_name": "Byrne", "given_name": "Margaret", "name": "Byrne, Margaret", "type": "personal" } }, { "affiliations": [ { "name": "University of Western Australia" } ], "person_or_org": { "family_name": "Cornish", "given_name": "Caitlin", "name": "Cornish, Caitlin", "type": "personal" } }, { "affiliations": [ { "name": "Department of Parks and Wildlife" } ], "person_or_org": { "family_name": "Kuchling", "given_name": "Gerald", "name": "Kuchling, Gerald", "type": "personal" } }, { "affiliations": [ { "name": "University of Western Australia" } ], "person_or_org": { "family_name": "Mitchell", "given_name": "Nicola", "name": "Mitchell, Nicola", "type": "personal" } }, { "affiliations": [ { "name": "Curtin University" } ], "person_or_org": { "family_name": "Jarman", "given_name": "Simon", "name": "Jarman, Simon", "type": "personal" } } ], "description": "Conservation translocations require robust post-release monitoring to evaluate their\u00a0success, which can be challenging to implement and maintain. Monitoring techniques that can account for the dispersal and cryptic nature of translocated animals are necessary to provide critical information on persistence and distribution. In this study, we developed a highly sensitive environmental DNA (eDNA) assay specific to the Critically Endangered western swamp turtle (Pseudemydura umbrina), a species currently undergoing trials of assisted colonisation. Actively filtering sufficient volumes of water in lentic systems is difficult due to high concentrations of clogging particulates, therefore we assessed the viability of passive sampling in a controlled environment by submerging filter membranes and directly extracting DNA. Active sampling detected P. umbrina with a 97.6% detection rate, whereas passive sampling resulted in an 8.3% detection rate. We then used a fine-scale eDNA sampling design and radio tracked translocated P. umbrina at the assisted colonisation wetland to investigate eDNA dispersal and spatial monitoring resolution. We detected P. umbrina at 42% (7 / 17) of eDNA sample sites, and the probability of a positive eDNA detection was negatively associated with the distance of P. umbrina from the sampling site, indicating limited eDNA dispersal from the source. Systems with low natural mixing and limited eDNA dispersal provide an opportunity for high resolution spatial and temporal monitoring via targeted eDNA approaches. This is beneficial for monitoring rare species in these systems, as such high-resolution results can provide insights on species presence, distribution, and microhabitat use.
", "publication_date": "2024-01-08", "publisher": "Zenodo", "resource_type": { "id": "dataset", "title": { "de": "Datensatz", "en": "Dataset" } }, "rights": [ { "description": { "en": "CC0 waives copyright interest in a work you've created and dedicates it to the world-wide public domain. Use CC0 to opt out of copyright entirely and ensure your work has the widest reach." }, "icon": "cc-cc0-icon", "id": "cc0-1.0", "props": { "scheme": "spdx", "url": "https://creativecommons.org/publicdomain/zero/1.0/legalcode" }, "title": { "en": "Creative Commons Zero v1.0 Universal" } } ], "subjects": [ { "subject": "freshwater turtle" }, { "subject": "eDNA" }, { "subject": "biomonitoring" }, { "subject": "Conservation translocation" }, { "subject": "passive eDNA" }, { "subject": "eDNA dispersal" } ], "title": "Environmental DNA reflects spatial distribution of a rare turtle in a lentic wetland assisted colonisation site" }, "parent": { "access": { "owned_by": { "user": "90070" }, "settings": { "accept_conditions_text": null, "allow_guest_requests": false, "allow_user_requests": false, "secret_link_expiration": 0 } }, "communities": { "default": "734b6452-1f40-4db0-b218-08ab1d568b7f", "entries": [ { "access": { "member_policy": "open", "members_visibility": "public", "record_submission_policy": "open", "review_policy": "open", "visibility": "public" }, "children": { "allow": false }, "created": "2020-02-04T14:03:17.992421+00:00", "custom_fields": {}, "deletion_status": { "is_deleted": false, "status": "P" }, "id": "734b6452-1f40-4db0-b218-08ab1d568b7f", "links": {}, "metadata": { "curation_policy": "The Dryad collection in Zenodo contains datasets mirrored from the Dryad Digital Repository. If you want your dataset to appear here, submit it through https://datadryad.org. No other datasets will be accepted.
", "description": "Dryad is an international open-access repository of research data, especially data underlying scientific and medical publications. Dryad is a curated general-purpose repository that makes data discoverable, freely reusable, and citable.", "organizations": [ { "id": "00x6h5n95" } ], "page": "Dryad is a community-governed open data publishing and curation repository. Through Dryad and Zenodo's partnership, Zenodo hosts a preservation copy of Dryad datasets, available through this Zenodo Community. For more information or to access the original published datasets, visit https://datadryad.org.
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