#This text file is the configuration file we used for our analyses. To generate valid pairs file, we use the scripts of an existing pipeline "Micro-Capture-C (MCC)" that is available for download at Oxford University Innovation Software Store. Further information of the pipeline is available at http://www.jdavieslab.com/Code.html. The configuration file is uploaded here as MicroC_analysis.txt ########################################################################################## #!/bin/bash # This part of the script allows you to run the pipe by executing the config.txt file perl /user/scripts/MicroC-analysis_pipeline_part1.pl -q -i ${0##*/} exit ########################################################################################## # Specifications ########################################################################################## # Insert path to the scripts /user/scripts master_folder= # Insert path that leads to genome_sizes.txt files bigwig_folder= # Insert the path for bowtie 2 to use for the reference genome bowtie_genome_path= # Insert the build of the genome you are using genome= ########################################################################################## # File specifications ########################################################################################## # NB the files must be named (filename)_R1.fastq (filename)_R2.fastq - specify the (filename) - without the _R1.fastq # Use fastq not fq # Avoid special characters in the filenames such as additional "."s or spaces file= reference= # The oligo file is a list of chrV-X of the yeast genome oligo_file= # Bed file specifying the color of the targets - must be in 8 column bed format with colour in the 8th column (r,g,b format) colour_file= ########################################################################################## # Parts of the first pipeline to run ########################################################################################## trim_galore=Y flash=Y fq2fa=Y blat=Y splitter=Y clean_wig=N gunzip=N gzip=N ########################################################################################## # Parts of the second pipe parameters ########################################################################################## Pipe2=Y bowtie=Y sort=Y MCCanal=Y postgzip=N track_hub=Y samtobam=N macs2=Y