Disrupting abnormal neuronal oscillations with adaptive delayed feedback control

This dataset contains the spike data of the experiments performed for the paper:

Domingos Leite de Castro, Miguel Aroso, A. Pedro Aguiar, David B. Grayden, Paulo Aguiar, "Disrupting abnormal neuronal oscillations with adaptive delayed feedback control", currently under review

You can access the pre-print at: https://doi.org/10.1101/2022.07.05.498735

The data was recorded with the 2100MEA-System from Multichannel Systems using their software Multichannel Experimenter.  Each h5 file contains the spike detection results for all electrodes, the electrode labels, and stimulation times. The experiments were performed with microelectrode arrays that contained multiple independent culture wells. The h5 files contain data recorded from all the wells, even though the experiments are only performed in one well at a time. To access the data of a given experiment, choose the electrodes associated with the well identified in the name of the folder, or consult the table in DataTable.xlsx.

The experiments followed both the European legislation regarding the use of animals for scientific purposes and the protocols approved by the ethical committee of i3S. The Animal Facility of i3S follows the FELASA guidelines and recommendations concerning laboratory animal welfare, complies with the European Guidelines (Directive 2010/63/EU) transposed to Portuguese legislation by Decreto-Lei no 113/2013 and is licensed by the Portuguese official veterinary department (DGAV, Ref 004461). Embryonic (E18) rat hippocampal neurons were cultured on 6-well or 9-well chamber MEA (256-6well MEA200/30iR-ITO-rcr and 256-9wellMEA300/30iR-ITO, respectively) (Multichannel System MCS, Germany) with a density of 5 × 104 cells/well and 3 x 104 cells/well, respectively. Each well of the 6-well MEA has 42 TiN electrodes (array of 7 × 6), and the 9-well contain arrays of 26 TiN electrodes (6 x 5 without corners). The experiments were performed between 13 and 55 days in vitro.