# Examining ozone susceptibility in the genus Musa (bananas)
[https://doi.org/10.5061/dryad.fbg79cp26](https://doi.org/10.5061/dryad.fbg79cp26)
The ozone (O3) susceptibility of cv. Williams was tested in nine independently controlled and monitored open top chambers (OTC) built at the UK University of Exeter’s TropOz Research facility located at James Cook University’s Environmental Research Complex (ERC) on the Nguma-bada campus in far-north Queensland, Australia ([www.tropoz.org](https://tropoz.org/)).
The plants (27 cv. Williams) were grown under O3 fumigation in OTCs for about three months. At the end of the O3 fumigation period, two leaves were collected from every plant, specifically the third most recently expanded and therefore newly mature leaf (new leaf) and the eighth-most recently expanded (old leaf) both new and old leaves having fully developed under O3 fumigation from every plant for the determination of leaf functional traits such as leaf mass per area (LMA), intrinsic water-use-efficiency (iWUE), total antioxidant capacity (TAC), and total phenolic contents (TPC).
LMA was calculated using the leaf dry mass (DM) obtained on freeze-dried samples and the leaf area (LA) determined by image analyser software (Image-J, NIH, [Bethesda, Maryland](https://en.wikipedia.org/wiki/Bethesda,_Maryland), USA). LMA was calculated as LMA= DM/LA in units of g m−2. TAC was determined in the leaf extract by the ferric reducing antioxidant power (FRAP) assay. TAC was expressed as ascorbic acid equivalents (mg AAE g−1 dry weight). Ascorbic acid was used as the standard. TPC was measured in the same leaf extract by the Folin–Ciocalteau method with some modifications. Gallic acid was used as a standard and TPC was expressed as Gallic acid equivalents (mg GAE g–1 dry weight). The carbon stable isotope ratio (δ13C, ‰) was determined using a Costech Elemental Analyser fitted with a zero-blank auto-sampler coupled via a ConFloIV to a ThermoFinnigan DeltaVPLUS using Continuous-Flow Isotope Ratio Mass Spectrometry (EA-IRMS) at James Cook University’s Advanced Analytical Centre. The δ13C values were used to calculate the iWUE.
At the end of the experiment, leaves, midrib, pseudostem, corm and small suckers were harvested separately, and dried in an oven at 70 °C until constant weight for biomass determination.
Values (dataset 1) represent OTC of three plants (n=3).
Environmental variables such as air temperature (T), air relative humidity (RH), shortwave radiation and photosynthetically active radiation (PAR) were monitored using a single meteorological monitoring station (Campbell Scientific, Logan, UT, USA) established in the central OTC.
Hourly values of O3 concentration and meteorological conditions were measured for the DO3SE (Deposition of O3 for Stomatal Exchange) model. The DO3SE model was used to estimate the O3 flux into leaves.
## Description of the data and file structure
Dataset was uploaded in three different excel sheets, Data1, Data2 and Data3 with their metadata (Data1\_Metadata, Data2\_Metadata, and Data3\_Metadata). Metadata sheets represents the parameter names, description, and units. Data1 sheet contains open top chamber averages data. Data2 sheet contains environmental variables during experimental period. Data3 sheet contains hourly values of O3 concentration and meteorological conditions that were measured for the DO3SE (Deposition of O3 for Stomatal Exchange) model.
## Sharing/Access information
Data was produced from our own experimental open top chambers (OTC) built at the UK University of Exeter’s TropOz Research facility located at James Cook University’s Environmental Research Complex (ERC) on the Nguma-bada campus in far-north Queensland, Australia ([www.tropoz.org](https://tropoz.org/)).
## Code/Software