RNAscope_macro

Relevant to Extended Data Figure 2

This ImageJ macro identifies RGCs with the RBPMS channel, then counts the FSTL4 and BNC2 puncta in each RGC.
The automated RGC ROI identification will require manual adjustment by the user.
Open the raw image file, then run the macro.

Ouput:

• The macro will output a list of mean fluorescence and number of puncta for each cell. These should be copied/saved.